CACNA1B cDNA ORF clone, Bos taurus(cattle)

The following CACNA1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa220147 XM_024998344.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa220138 XM_024998335.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220139 XM_024998336.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220140 XM_024998337.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220141 XM_024998338.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220142 XM_024998339.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220143 XM_024998340.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220144 XM_024998341.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa220145 XM_024998342.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa220146 XM_024998343.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa220148 XM_024998345.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa36127 NM_174632.2
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa220147
    Clone ID Related Accession (Same CDS sequence) XM_024998344.1
    Accession Version XM_024998344.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGCAGTGTT GAAGAGAGCA
    GCCACCAAGA AGAGCCGAAA CGACCTGATC CACGCGGAGG AGGGAGAGGA CCGCTTTGCA
    GACCTCTGTG CTGTGGGGTC CCCCTTCGCC CGAGCCAGCC TCAAGAGTGG GAAGACGGAG
    AGCTCGTCAT ACTTCCGCAG GAAGGAGAAG ATGTTCCGGT TCTTCATCCG GCGCCTGGTG
    AAGGCGCAGA GCTTCTACTG GGTGGTGCTG TGTGTGGTGG CCCTGAACAC GCTGTGCGTG
    GCCATGGTGC ACTACCAGCA GCCCCAGCGG CTCACCACGG CGCTGTACTT TGCAGAGTTT
    GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGG
    AGCTACTTCC GGTCCTCCTT CAACTGCTTC GATTTCGGGG TCATTGTGGG GAGCATTTTC
    GAAGTGGTCT GGGCGGCCAT CAAGCCAGGA ACCTCCTTCG GCATCAGTGT GCTGCGGGCG
    CTGCGGCTGC TGAGAATCTT CAAAGTCACC AAGTACTGGA GCTCCCTGCG GAACCTGGTG
    GTGTCCCTGC TCAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCCTGCT CTTCCTCTTC
    ATCGTGGTCT TCGCTCTGCT GGGCATGCAG CTCTTCGGGG GACAGTTCAA CTTCCAGGAT
    GAGACTCCGA CAACCAACTT TGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC
    CTGACCGGGG AGGACTGGAA TGCGGTGATG TACCATGGGA TCGAATCACA AGGTGGAGTC
    AGCAAGGGCA TGTTCTCGTC TGTGTACTTC ATTGTCCTGA CGCTGTTTGG GAACTACACC
    CTGCTGAACG TCTTCCTGGC CATCGCTGTG GACAACCTCG CCAATGCTCA GGAGCTGACC
    AAGGACGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA
    GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATTGCTGC CAGGCAGCAG
    AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTGCG GCTGCAGAAC
    CTGCGGGCCA GCTGTGAAGC GCTGTATAGC GAGATGGACC CCGAGGAGCG CCTGCGCTTC
    GCCACTTCCC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTTGTGGTG
    GAGCCAGGCC GGGATGGCGC GCGGGGGCCT GCGGGGGGCA AGGCCCGGCC CGAGGGCGGG
    GAGGCCGGCG AGGGTGCCGA CCCGCCGCGC AGGCACCACC GGCACCGCGA CAGGGACAGG
    GACAAGGCCG CAGCCCCGGC GGGGGAGCCA GACAGGGCGG ACGCCCCGAA GGCGGAGGGC
    GGGGAGCCGG GGGCCCGGGA GGAGCGGGCG CGACCGCGCC GCAGCCGCAG CAAGGAGGCC
    GCAGGCCCCC GCGAGGCCAG GAGCGAGCGA GGCCGCGGGC TGGGCCCGGA CGGCGGCCGG
    CGCCACCACC GGCGCGGCTC CCCGGAGGAG GCCGCGGAGC GCGAGCCCCG GCGCCACCGC
    GCGCACCGGC ACGCGCCGGA GCCGAGCAGG GAGGGCGCGC CCGGGAGCAA GGGCGAGCGG
    CGAGCGCGGC ACCGCGGCGG CTCCCGGGCC GGGCCCCGGG AGGCCGAGAG CGGCGAGGAG
    CCCTCCCGGC GGCACCGAGC GCGACACAAG GCGCCGCCCG CACACGAGGA GGCGGAGAAG
    GAGGCCGAGG CGGAGGACAG GGACAAGGAG CCCCGAAACC ACCAGCCCAG GGAGTCGCAC
    TGTGACCTGG AGGCCAGTGT GGTCATTGGT GTGGGCCCTG TGCATGCCCT GCCCAGCACT
    TGTCTAGAGA AGGTGGAAGA GCAGCCAGAG GATGCAGACA ATCAGCGGAA TGTGACTCGC
    ATGGGCAGTC AGCCCTCAGA TCTGAGCACC GCTGTGCGTA TCCCAGTGAC GCTGACCGGG
    CCTCCCGGGG AGACCACGGT TGTTCCCAGT GGTAACGTGG ACCTGGAAAG CCAAGCAGAA
    GGAAAGAAGG AGGTGGAAGC TGATGACGTG ATGAGGAGTG GCCCCCGACC CATTGTCCCG
    TACAGCTCCA TGTTCTGCTT GAGCCCCACC AACCTGCTCC GCCGCTTCTG CCACTACATT
    GTGACCATGC GGTACTTCGA GATGGTCATT CTCGTGGTCA TTGCCCTGAG TAGCATTGCC
    CTGGCTGCGG AGGATCCTGT GCAGACAGAC TCGCCCAGGA ACAACGTGCT GAAGTACATG
    GACTACATCT TCACAGGTGT CTTCACCTTT GAGATGGTGA TAAAGATGAT CGATTTGGGA
    CTGCTGCTGC ACCCCGGTGC CTACTTCCGG GATCTGTGGA ACATTCTGGA CTTCATTGTG
    GTCAGTGGGG CCCTGGTGGC GTTTGCCTTC TCGAGCTTCA TGGGAGGCTC CAAAGGGAAA
    GACATCAGCA CCATCAAGTC CCTGAGGGTC CTGCGTGTGC TGAGGCCACT CAAGACCATC
    AAGCGGCTGC CCAAGCTCAA AGCTGTGTTT GACTGTGTGG TGAACTCCCT GAAGAACGTC
    CTCAACATCC TGATCGTCTA CATGCTCTTC ATGTTCATAT TTGCTGTCAT CGCGGTACAG
    CTCTTCAAGG GGAAGTTTTT CTACTGCACC GACGAGTCCA AGGAGCTGGA GCGGGACTGC
    AGGGGCCAGT ATTTGGACTA CGAGAAGGAG GAGGTGGAAG CTCAGCCACG GCAGTGGAAG
    AAATACGACT TCCACTATGA CAACGTGCTG TGGGCCCTGC TAACGCTGTT CACTGTGTCC
    ACGGGCGAAG GGTGGCCCAT GGTGCTGAAA CACTCGGTGG ATGCCACCTA CGAGGAGCAG
    GGCCCCAGCC CCGGGTTCCG CATGGAGCTG TCCATCTTCT ATGTGGTCTA CTTCGTGGTC
    TTCCCCTTCT TCTTTGTGAA CATCTTTGTG GCCTTGATCA TCATCACCTT CCAGGAGCAG
    GGGGACAAAG TCATGTCTGA GTGCAGCTTG GAGAAGAACG AGAGGGCTTG TATCGACTTC
    GCCATTAGCG CCAAACCCCT GACGCGGTAC ATGCCCCAGA ACAAGCAGTC GTTCCAGTAC
    AAGACGTGGA CGTTCGTGGT CTCACCACCC TTTGAGTATT TCATCATGGC CATGATAGCC
    CTCAACACAG TGGTGCTGAT GATGAAGTTC TACGATGCGC CCTACGAGTA CGAACTGATG
    TTGAAGTGTC TGAACATCGT GTTCACGTCC ATGTTCTCCA TGGAGTGTGT GCTCAAGGTC
    ATCGCCTTTG GAGTGCTGAA CTATTTCAGA GACGCCTGGA ATGTCTTTGA CTTTGTCACT
    GTGTTGGGAA GTATTACTGA TATTTTAGTA ACAGAGATTG CGGAAACGAA CAACTTCATC
    AACCTCAGCT TCCTCCGCCT CTTCCGTGCT GCCCGACTCA TCAAGCTGCT CCGCCAAGGC
    TACACCATCC GCATTCTGCT CTGGACCTTC GTCCAGTCCT TCAAGGCCCT GCCCTATGTG
    TGCCTGCTCA TCGCCATGCT GTTCTTCATC TACGCCATCA TCGGCATGCA GGTGTTTGGA
    AACATTGCTC TGGATGACGA CACCAGCATC AACCGTCACA ATAACTTTCG GACATTTCTT
    CAGGCCTTGA TGCTGCTCTT CAGGAGTGCC ACCGGGGAGG CCTGGCATGA GATCATGCTG
    TCCTGCCTCA GCAGTCGGGC CTGTGATGAG CTCGCCAATG CCACGGAGTG TGGGAGTGAC
    TTCGCCTACT TCTACTTTGT CTCCTTCATC TTCCTTTGCT CCTTTCTGAT GTTGAACCTC
    TTTGTGGCTG TGATCATGGA CAATTTTGAG TACCTTACGC GGGACTCTTC CATCCTAGGG
    CCTCACCACC TGGACGAATT CATCCGGGTC TGGGCCGAGT ACGACCCGGC TGCGTGCTGG
    CACGAAGCAG CATCAGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854112.1
    CDS146..5386
    Translation

    Target ORF information:

    RefSeq Version XM_024998344.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998344.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGCAGTGTT GAAGAGAGCA
    GCCACCAAGA AGAGCCGAAA CGACCTGATC CACGCGGAGG AGGGAGAGGA CCGCTTTGCA
    GACCTCTGTG CTGTGGGGTC CCCCTTCGCC CGAGCCAGCC TCAAGAGTGG GAAGACGGAG
    AGCTCGTCAT ACTTCCGCAG GAAGGAGAAG ATGTTCCGGT TCTTCATCCG GCGCCTGGTG
    AAGGCGCAGA GCTTCTACTG GGTGGTGCTG TGTGTGGTGG CCCTGAACAC GCTGTGCGTG
    GCCATGGTGC ACTACCAGCA GCCCCAGCGG CTCACCACGG CGCTGTACTT TGCAGAGTTT
    GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGG
    AGCTACTTCC GGTCCTCCTT CAACTGCTTC GATTTCGGGG TCATTGTGGG GAGCATTTTC
    GAAGTGGTCT GGGCGGCCAT CAAGCCAGGA ACCTCCTTCG GCATCAGTGT GCTGCGGGCG
    CTGCGGCTGC TGAGAATCTT CAAAGTCACC AAGTACTGGA GCTCCCTGCG GAACCTGGTG
    GTGTCCCTGC TCAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCCTGCT CTTCCTCTTC
    ATCGTGGTCT TCGCTCTGCT GGGCATGCAG CTCTTCGGGG GACAGTTCAA CTTCCAGGAT
    GAGACTCCGA CAACCAACTT TGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC
    CTGACCGGGG AGGACTGGAA TGCGGTGATG TACCATGGGA TCGAATCACA AGGTGGAGTC
    AGCAAGGGCA TGTTCTCGTC TGTGTACTTC ATTGTCCTGA CGCTGTTTGG GAACTACACC
    CTGCTGAACG TCTTCCTGGC CATCGCTGTG GACAACCTCG CCAATGCTCA GGAGCTGACC
    AAGGACGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA
    GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATTGCTGC CAGGCAGCAG
    AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTGCG GCTGCAGAAC
    CTGCGGGCCA GCTGTGAAGC GCTGTATAGC GAGATGGACC CCGAGGAGCG CCTGCGCTTC
    GCCACTTCCC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTTGTGGTG
    GAGCCAGGCC GGGATGGCGC GCGGGGGCCT GCGGGGGGCA AGGCCCGGCC CGAGGGCGGG
    GAGGCCGGCG AGGGTGCCGA CCCGCCGCGC AGGCACCACC GGCACCGCGA CAGGGACAGG
    GACAAGGCCG CAGCCCCGGC GGGGGAGCCA GACAGGGCGG ACGCCCCGAA GGCGGAGGGC
    GGGGAGCCGG GGGCCCGGGA GGAGCGGGCG CGACCGCGCC GCAGCCGCAG CAAGGAGGCC
    GCAGGCCCCC GCGAGGCCAG GAGCGAGCGA GGCCGCGGGC TGGGCCCGGA CGGCGGCCGG
    CGCCACCACC GGCGCGGCTC CCCGGAGGAG GCCGCGGAGC GCGAGCCCCG GCGCCACCGC
    GCGCACCGGC ACGCGCCGGA GCCGAGCAGG GAGGGCGCGC CCGGGAGCAA GGGCGAGCGG
    CGAGCGCGGC ACCGCGGCGG CTCCCGGGCC GGGCCCCGGG AGGCCGAGAG CGGCGAGGAG
    CCCTCCCGGC GGCACCGAGC GCGACACAAG GCGCCGCCCG CACACGAGGA GGCGGAGAAG
    GAGGCCGAGG CGGAGGACAG GGACAAGGAG CCCCGAAACC ACCAGCCCAG GGAGTCGCAC
    TGTGACCTGG AGGCCAGTGT GGTCATTGGT GTGGGCCCTG TGCATGCCCT GCCCAGCACT
    TGTCTAGAGA AGGTGGAAGA GCAGCCAGAG GATGCAGACA ATCAGCGGAA TGTGACTCGC
    ATGGGCAGTC AGCCCTCAGA TCTGAGCACC GCTGTGCGTA TCCCAGTGAC GCTGACCGGG
    CCTCCCGGGG AGACCACGGT TGTTCCCAGT GGTAACGTGG ACCTGGAAAG CCAAGCAGAA
    GGAAAGAAGG AGGTGGAAGC TGATGACGTG ATGAGGAGTG GCCCCCGACC CATTGTCCCG
    TACAGCTCCA TGTTCTGCTT GAGCCCCACC AACCTGCTCC GCCGCTTCTG CCACTACATT
    GTGACCATGC GGTACTTCGA GATGGTCATT CTCGTGGTCA TTGCCCTGAG TAGCATTGCC
    CTGGCTGCGG AGGATCCTGT GCAGACAGAC TCGCCCAGGA ACAACGTGCT GAAGTACATG
    GACTACATCT TCACAGGTGT CTTCACCTTT GAGATGGTGA TAAAGATGAT CGATTTGGGA
    CTGCTGCTGC ACCCCGGTGC CTACTTCCGG GATCTGTGGA ACATTCTGGA CTTCATTGTG
    GTCAGTGGGG CCCTGGTGGC GTTTGCCTTC TCGAGCTTCA TGGGAGGCTC CAAAGGGAAA
    GACATCAGCA CCATCAAGTC CCTGAGGGTC CTGCGTGTGC TGAGGCCACT CAAGACCATC
    AAGCGGCTGC CCAAGCTCAA AGCTGTGTTT GACTGTGTGG TGAACTCCCT GAAGAACGTC
    CTCAACATCC TGATCGTCTA CATGCTCTTC ATGTTCATAT TTGCTGTCAT CGCGGTACAG
    CTCTTCAAGG GGAAGTTTTT CTACTGCACC GACGAGTCCA AGGAGCTGGA GCGGGACTGC
    AGGGGCCAGT ATTTGGACTA CGAGAAGGAG GAGGTGGAAG CTCAGCCACG GCAGTGGAAG
    AAATACGACT TCCACTATGA CAACGTGCTG TGGGCCCTGC TAACGCTGTT CACTGTGTCC
    ACGGGCGAAG GGTGGCCCAT GGTGCTGAAA CACTCGGTGG ATGCCACCTA CGAGGAGCAG
    GGCCCCAGCC CCGGGTTCCG CATGGAGCTG TCCATCTTCT ATGTGGTCTA CTTCGTGGTC
    TTCCCCTTCT TCTTTGTGAA CATCTTTGTG GCCTTGATCA TCATCACCTT CCAGGAGCAG
    GGGGACAAAG TCATGTCTGA GTGCAGCTTG GAGAAGAACG AGAGGGCTTG TATCGACTTC
    GCCATTAGCG CCAAACCCCT GACGCGGTAC ATGCCCCAGA ACAAGCAGTC GTTCCAGTAC
    AAGACGTGGA CGTTCGTGGT CTCACCACCC TTTGAGTATT TCATCATGGC CATGATAGCC
    CTCAACACAG TGGTGCTGAT GATGAAGTTC TACGATGCGC CCTACGAGTA CGAACTGATG
    TTGAAGTGTC TGAACATCGT GTTCACGTCC ATGTTCTCCA TGGAGTGTGT GCTCAAGGTC
    ATCGCCTTTG GAGTGCTGAA CTATTTCAGA GACGCCTGGA ATGTCTTTGA CTTTGTCACT
    GTGTTGGGAA GTATTACTGA TATTTTAGTA ACAGAGATTG CGGAAACGAA CAACTTCATC
    AACCTCAGCT TCCTCCGCCT CTTCCGTGCT GCCCGACTCA TCAAGCTGCT CCGCCAAGGC
    TACACCATCC GCATTCTGCT CTGGACCTTC GTCCAGTCCT TCAAGGCCCT GCCCTATGTG
    TGCCTGCTCA TCGCCATGCT GTTCTTCATC TACGCCATCA TCGGCATGCA GGTGTTTGGA
    AACATTGCTC TGGATGACGA CACCAGCATC AACCGTCACA ATAACTTTCG GACATTTCTT
    CAGGCCTTGA TGCTGCTCTT CAGGAGTGCC ACCGGGGAGG CCTGGCATGA GATCATGCTG
    TCCTGCCTCA GCAGTCGGGC CTGTGATGAG CTCGCCAATG CCACGGAGTG TGGGAGTGAC
    TTCGCCTACT TCTACTTTGT CTCCTTCATC TTCCTTTGCT CCTTTCTGAT GTTGAACCTC
    TTTGTGGCTG TGATCATGGA CAATTTTGAG TACCTTACGC GGGACTCTTC CATCCTAGGG
    CCTCACCACC TGGACGAATT CATCCGGGTC TGGGCCGAGT ACGACCCGGC TGCGTGCTGG
    CACGAAGCAG CATCAGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220138
    Clone ID Related Accession (Same CDS sequence) XM_024998335.1
    Accession Version XM_024998335.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGCAGTGTT GAAGAGAGCA
    GCCACCAAGA AGAGCCGAAA CGACCTGATC CACGCGGAGG AGGGAGAGGA CCGCTTTGCA
    GACCTCTGTG CTGTGGGGTC CCCCTTCGCC CGAGCCAGCC TCAAGAGTGG GAAGACGGAG
    AGCTCGTCAT ACTTCCGCAG GAAGGAGAAG ATGTTCCGGT TCTTCATCCG GCGCCTGGTG
    AAGGCGCAGA GCTTCTACTG GGTGGTGCTG TGTGTGGTGG CCCTGAACAC GCTGTGCGTG
    GCCATGGTGC ACTACCAGCA GCCCCAGCGG CTCACCACGG CGCTGTACTT TGCAGAGTTT
    GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGG
    AGCTACTTCC GGTCCTCCTT CAACTGCTTC GATTTCGGGG TCATTGTGGG GAGCATTTTC
    GAAGTGGTCT GGGCGGCCAT CAAGCCAGGA ACCTCCTTCG GCATCAGTGT GCTGCGGGCG
    CTGCGGCTGC TGAGAATCTT CAAAGTCACC AAGTACTGGA GCTCCCTGCG GAACCTGGTG
    GTGTCCCTGC TCAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCCTGCT CTTCCTCTTC
    ATCGTGGTCT TCGCTCTGCT GGGCATGCAG CTCTTCGGGG GACAGTTCAA CTTCCAGGAT
    GAGACTCCGA CAACCAACTT TGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC
    CTGACCGGGG AGGACTGGAA TGCGGTGATG TACCATGGGA TCGAATCACA AGGTGGAGTC
    AGCAAGGGCA TGTTCTCGTC TGTGTACTTC ATTGTCCTGA CGCTGTTTGG GAACTACACC
    CTGCTGAACG TCTTCCTGGC CATCGCTGTG GACAACCTCG CCAATGCTCA GGAGCTGACC
    AAGGACGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA
    GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATTGCTGC CAGGCAGCAG
    AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTGCG GCTGCAGAAC
    CTGCGGGCCA GCTGTGAAGC GCTGTATAGC GAGATGGACC CCGAGGAGCG CCTGCGCTTC
    GCCACTTCCC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTTGTGGTG
    GAGCCAGGCC GGGATGGCGC GCGGGGGCCT GCGGGGGGCA AGGCCCGGCC CGAGGGCGGG
    GAGGCCGGCG AGGGTGCCGA CCCGCCGCGC AGGCACCACC GGCACCGCGA CAGGGACAGG
    GACAAGGCCG CAGCCCCGGC GGGGGAGCCA GACAGGGCGG ACGCCCCGAA GGCGGAGGGC
    GGGGAGCCGG GGGCCCGGGA GGAGCGGGCG CGACCGCGCC GCAGCCGCAG CAAGGAGGCC
    GCAGGCCCCC GCGAGGCCAG GAGCGAGCGA GGCCGCGGGC TGGGCCCGGA CGGCGGCCGG
    CGCCACCACC GGCGCGGCTC CCCGGAGGAG GCCGCGGAGC GCGAGCCCCG GCGCCACCGC
    GCGCACCGGC ACGCGCCGGA GCCGAGCAGG GAGGGCGCGC CCGGGAGCAA GGGCGAGCGG
    CGAGCGCGGC ACCGCGGCGG CTCCCGGGCC GGGCCCCGGG AGGCCGAGAG CGGCGAGGAG
    CCCTCCCGGC GGCACCGAGC GCGACACAAG GCGCCGCCCG CACACGAGGA GGCGGAGAAG
    GAGGCCGAGG CGGAGGACAG GGACAAGGAG CCCCGAAACC ACCAGCCCAG GGAGTCGCAC
    TGTGACCTGG AGGCCAGTGT GGTCATTGGT GTGGGCCCTG TGCATGCCCT GCCCAGCACT
    TGTCTAGAGA AGGTGGAAGA GCAGCCAGAG GATGCAGACA ATCAGCGGAA TGTGACTCGC
    ATGGGCAGTC AGCCCTCAGA TCTGAGCACC GCTGTGCGTA TCCCAGTGAC GCTGACCGGG
    CCTCCCGGGG AGACCACGGT TGTTCCCAGT GGTAACGTGG ACCTGGAAAG CCAAGCAGAA
    GGAAAGAAGG AGGTGGAAGC TGATGACGTG ATGAGGAGTG GCCCCCGACC CATTGTCCCG
    TACAGCTCCA TGTTCTGCTT GAGCCCCACC AACCTGCTCC GCCGCTTCTG CCACTACATT
    GTGACCATGC GGTACTTCGA GATGGTCATT CTCGTGGTCA TTGCCCTGAG TAGCATTGCC
    CTGGCTGCGG AGGATCCTGT GCAGACAGAC TCGCCCAGGA ACAACGTGCT GAAGTACATG
    GACTACATCT TCACAGGTGT CTTCACCTTT GAGATGGTGA TAAAGATGAT CGATTTGGGA
    CTGCTGCTGC ACCCCGGTGC CTACTTCCGG GATCTGTGGA ACATTCTGGA CTTCATTGTG
    GTCAGTGGGG CCCTGGTGGC GTTTGCCTTC TCGAGCTTCA TGGGAGGCTC CAAAGGGAAA
    GACATCAGCA CCATCAAGTC CCTGAGGGTC CTGCGTGTGC TGAGGCCACT CAAGACCATC
    AAGCGGCTGC CCAAGCTCAA AGCTGTGTTT GACTGTGTGG TGAACTCCCT GAAGAACGTC
    CTCAACATCC TGATCGTCTA CATGCTCTTC ATGTTCATAT TTGCTGTCAT CGCGGTACAG
    CTCTTCAAGG GGAAGTTTTT CTACTGCACC GACGAGTCCA AGGAGCTGGA GCGGGACTGC
    AGGGGCCAGT ATTTGGACTA CGAGAAGGAG GAGGTGGAAG CTCAGCCACG GCAGTGGAAG
    AAATACGACT TCCACTATGA CAACGTGCTG TGGGCCCTGC TAACGCTGTT CACTGTGTCC
    ACGGGCGAAG GGTGGCCCAT GGTGCTGAAA CACTCGGTGG ATGCCACCTA CGAGGAGCAG
    GGCCCCAGCC CCGGGTTCCG CATGGAGCTG TCCATCTTCT ATGTGGTCTA CTTCGTGGTC
    TTCCCCTTCT TCTTTGTGAA CATCTTTGTG GCCTTGATCA TCATCACCTT CCAGGAGCAG
    GGGGACAAAG TCATGTCTGA GTGCAGCTTG GAGAAGAACG AGAGGGCTTG TATCGACTTC
    GCCATTAGCG CCAAACCCCT GACGCGGTAC ATGCCCCAGA ACAAGCAGTC GTTCCAGTAC
    AAGACGTGGA CGTTCGTGGT CTCACCACCC TTTGAGTATT TCATCATGGC CATGATAGCC
    CTCAACACAG TGGTGCTGAT GATGAAGTTC TACGATGCGC CCTACGAGTA CGAACTGATG
    TTGAAGTGTC TGAACATCGT GTTCACGTCC ATGTTCTCCA TGGAGTGTGT GCTCAAGGTC
    ATCGCCTTTG GAGTGCTGAA CTATTTCAGA GACGCCTGGA ATGTCTTTGA CTTTGTCACT
    GTGTTGGGAA GTATTACTGA TATTTTAGTA ACAGAGATTG CGGAAACGAA CAACTTCATC
    AACCTCAGCT TCCTCCGCCT CTTCCGTGCT GCCCGACTCA TCAAGCTGCT CCGCCAAGGC
    TACACCATCC GCATTCTGCT CTGGACCTTC GTCCAGTCCT TCAAGGCCCT GCCCTATGTG
    TGCCTGCTCA TCGCCATGCT GTTCTTCATC TACGCCATCA TCGGCATGCA GGTGTTTGGA
    AACATTGCTC TGGATGACGA CACCAGCATC AACCGTCACA ATAACTTTCG GACATTTCTT
    CAGGCCTTGA TGCTGCTCTT CAGGAGTGCC ACCGGGGAGG CCTGGCATGA GATCATGCTG
    TCCTGCCTCA GCAGTCGGGC CTGTGATGAG CTCGCCAATG CCACGGAGTG TGGGAGTGAC
    TTCGCCTACT TCTACTTTGT CTCCTTCATC TTCCTTTGCT CCTTTCTGAT GTTGAACCTC
    TTTGTGGCTG TGATCATGGA CAATTTTGAG TACCTTACGC GGGACTCTTC CATCCTAGGG
    CCTCACCACC TGGACGAATT CATCCGGGTC TGGGCCGAGT ACGACCCGGC TGCGTGTGGG
    CGCATCAGTT ACAATGACAT GTTTGAGATG CTGAAACACA TGTCCCCGCC CCTGGGGCTG
    GGGAAGAAAT GCCCTGCTCG TGTTGCGTAC AAGCGCCTGG TTCGTATGAA CATGCCCATC
    TCCAATGACG ACATGACCGT CCACTTCACA TCCACGCTGA TGGCCCTCAT CCGGACTGCA
    TTGGAGATCA AGCTGGCTCC AGCTGGCACG AAGCAGCATC AGTGTGACGC AGAGCTGAGG
    AAGGAGATCT CCTCCGTGTG GGCCAATCTG CCCCAGAAGA CCCTGGACTT ACTGGTGCCA
    CCCCATAAAC CCGACGAGAT GACGGTGGGG AAGGTGTACG CAGCTCTGAT GATATTCGAC
    TTCTACAAAC AGAACAAAAC CACCAGAGAC CAGATTCACC AAGCTCCTGG AGGCCTGGCC
    CAGATGGGCC CCGTGTCCCT GTTCCACCCC CTGAAGGCCA CGCTGGAGCA GACTCAGCCG
    GCTGTGCTCC GAGGAGCCCG CGTTTTCCTT CGGCAGAAGA GCTCGGCCTC CCTCAGCAAC
    GGTGGGGCAG TACAAACCCA AGAGGGCGGC ATCAAGGAGT CTGTCTCCTG GGGCACTCAG
    AGGACCCAGG AGGTCCCCTG CGAGGTGAGG ACACCCCTGG AGCGTGGCCA CTCCACAGAG
    ATCCCGGTGC AGACAGGAAA ACCGGCTGTG GACGTCCAGA TGCAGAGCAT GGTCCTGAGA
    GGCCCTGACG GGGAGCCCCA ACCTGGGCTG GAGAGCCAGG GCCGAGCAGC TTCCATGCCC
    CGCCTGGCAG CAGAGACTCA GCCACCCCCA GATGCCAGCC CCATGAAGCG CTCCATCTCC
    ACACTGGCAC CCCAGAGGCC CCATGTGGCT CATCTGTGCA CCGCGGCCCT GGACCGCGCT
    CCAGCCAGCC AGGCCGCCCC CCCCCACCAC CACCGCTGCC ATCGCCGCAG GGACCGGAAG
    CAGAAGTCCC TGGAGAAGGG GCCCGGCCTG TCTGCAGACA CAGATGGCGC GCCCTACAGC
    ACCGCGGGGC CGGGCCCCCC GCCGCCCCCA GGGGATGGGG CAGCAGGCTG CCGGCGGGAG
    CGCAGGCAGG AGCGGGGCCG GTCCCAGGAG CGGAGGCAGC CCTCCTCCTC TTCCTCTGAG
    AAGCAGCGCT TCTACTCCTG TGACCGCTTT GGGGGCCGTG AGCCCCCGCA GCCCAAGCCC
    TCTCTCAGCA GCCATCCCAC GTCGCCCACG GCCGGGCAGG AGCCAGGACC CCCGAGACAG
    GGTAGCGGCT CCGTGAACGG GAGCCCCCTG CTGTCGACAT CTGGTGCTAG CACCCCTGGC
    CGCGGCGGGC GGAGGCAGCT CCCCCAGACC CCGCTGACCC CCCGTCCCAG CATCACCTAT
    AAGACGGCCA ACTCCTCACC TGTCCACTTT GCCGGGGCTC AGACCAGCCT CCCTGCCTTT
    TCCCCCGGCC GGCTTAGCCG TGGCCTTTCG GAACACAACG CCCTGCTCCA GAGAGACCCC
    CTCAGCCAGC CGCTGGCCCC CAGCTCTCGG ATTGGCTCCG ACCCTTACCT GGGGCAGCGT
    CTGGACAGTG AGGCTGCGGC CCGCACCCAG CTTGAGGACA CGCTCACCTT CGAGGAGGCC
    GTGGCCACCA ACTCGGGCCG CTCCTCCCGG ACTTCCTACG TGTCCTCCCT GACCTCGCAG
    TCCCACCCCC TCCGCCGGGT GCCCAACGGC TACCACTGCA CTCTGGGGCT CAGCTCGGGC
    GGGGGCGGCC GGGGGCGGCA CAGCTACCAC CACCCTGACC AAGACCACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854103.1
    CDS140..7156
    Translation

    Target ORF information:

    RefSeq Version XM_024998335.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998335.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGCAGTGTT GAAGAGAGCA
    GCCACCAAGA AGAGCCGAAA CGACCTGATC CACGCGGAGG AGGGAGAGGA CCGCTTTGCA
    GACCTCTGTG CTGTGGGGTC CCCCTTCGCC CGAGCCAGCC TCAAGAGTGG GAAGACGGAG
    AGCTCGTCAT ACTTCCGCAG GAAGGAGAAG ATGTTCCGGT TCTTCATCCG GCGCCTGGTG
    AAGGCGCAGA GCTTCTACTG GGTGGTGCTG TGTGTGGTGG CCCTGAACAC GCTGTGCGTG
    GCCATGGTGC ACTACCAGCA GCCCCAGCGG CTCACCACGG CGCTGTACTT TGCAGAGTTT
    GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGG
    AGCTACTTCC GGTCCTCCTT CAACTGCTTC GATTTCGGGG TCATTGTGGG GAGCATTTTC
    GAAGTGGTCT GGGCGGCCAT CAAGCCAGGA ACCTCCTTCG GCATCAGTGT GCTGCGGGCG
    CTGCGGCTGC TGAGAATCTT CAAAGTCACC AAGTACTGGA GCTCCCTGCG GAACCTGGTG
    GTGTCCCTGC TCAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCCTGCT CTTCCTCTTC
    ATCGTGGTCT TCGCTCTGCT GGGCATGCAG CTCTTCGGGG GACAGTTCAA CTTCCAGGAT
    GAGACTCCGA CAACCAACTT TGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC
    CTGACCGGGG AGGACTGGAA TGCGGTGATG TACCATGGGA TCGAATCACA AGGTGGAGTC
    AGCAAGGGCA TGTTCTCGTC TGTGTACTTC ATTGTCCTGA CGCTGTTTGG GAACTACACC
    CTGCTGAACG TCTTCCTGGC CATCGCTGTG GACAACCTCG CCAATGCTCA GGAGCTGACC
    AAGGACGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA
    GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATTGCTGC CAGGCAGCAG
    AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTGCG GCTGCAGAAC
    CTGCGGGCCA GCTGTGAAGC GCTGTATAGC GAGATGGACC CCGAGGAGCG CCTGCGCTTC
    GCCACTTCCC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTTGTGGTG
    GAGCCAGGCC GGGATGGCGC GCGGGGGCCT GCGGGGGGCA AGGCCCGGCC CGAGGGCGGG
    GAGGCCGGCG AGGGTGCCGA CCCGCCGCGC AGGCACCACC GGCACCGCGA CAGGGACAGG
    GACAAGGCCG CAGCCCCGGC GGGGGAGCCA GACAGGGCGG ACGCCCCGAA GGCGGAGGGC
    GGGGAGCCGG GGGCCCGGGA GGAGCGGGCG CGACCGCGCC GCAGCCGCAG CAAGGAGGCC
    GCAGGCCCCC GCGAGGCCAG GAGCGAGCGA GGCCGCGGGC TGGGCCCGGA CGGCGGCCGG
    CGCCACCACC GGCGCGGCTC CCCGGAGGAG GCCGCGGAGC GCGAGCCCCG GCGCCACCGC
    GCGCACCGGC ACGCGCCGGA GCCGAGCAGG GAGGGCGCGC CCGGGAGCAA GGGCGAGCGG
    CGAGCGCGGC ACCGCGGCGG CTCCCGGGCC GGGCCCCGGG AGGCCGAGAG CGGCGAGGAG
    CCCTCCCGGC GGCACCGAGC GCGACACAAG GCGCCGCCCG CACACGAGGA GGCGGAGAAG
    GAGGCCGAGG CGGAGGACAG GGACAAGGAG CCCCGAAACC ACCAGCCCAG GGAGTCGCAC
    TGTGACCTGG AGGCCAGTGT GGTCATTGGT GTGGGCCCTG TGCATGCCCT GCCCAGCACT
    TGTCTAGAGA AGGTGGAAGA GCAGCCAGAG GATGCAGACA ATCAGCGGAA TGTGACTCGC
    ATGGGCAGTC AGCCCTCAGA TCTGAGCACC GCTGTGCGTA TCCCAGTGAC GCTGACCGGG
    CCTCCCGGGG AGACCACGGT TGTTCCCAGT GGTAACGTGG ACCTGGAAAG CCAAGCAGAA
    GGAAAGAAGG AGGTGGAAGC TGATGACGTG ATGAGGAGTG GCCCCCGACC CATTGTCCCG
    TACAGCTCCA TGTTCTGCTT GAGCCCCACC AACCTGCTCC GCCGCTTCTG CCACTACATT
    GTGACCATGC GGTACTTCGA GATGGTCATT CTCGTGGTCA TTGCCCTGAG TAGCATTGCC
    CTGGCTGCGG AGGATCCTGT GCAGACAGAC TCGCCCAGGA ACAACGTGCT GAAGTACATG
    GACTACATCT TCACAGGTGT CTTCACCTTT GAGATGGTGA TAAAGATGAT CGATTTGGGA
    CTGCTGCTGC ACCCCGGTGC CTACTTCCGG GATCTGTGGA ACATTCTGGA CTTCATTGTG
    GTCAGTGGGG CCCTGGTGGC GTTTGCCTTC TCGAGCTTCA TGGGAGGCTC CAAAGGGAAA
    GACATCAGCA CCATCAAGTC CCTGAGGGTC CTGCGTGTGC TGAGGCCACT CAAGACCATC
    AAGCGGCTGC CCAAGCTCAA AGCTGTGTTT GACTGTGTGG TGAACTCCCT GAAGAACGTC
    CTCAACATCC TGATCGTCTA CATGCTCTTC ATGTTCATAT TTGCTGTCAT CGCGGTACAG
    CTCTTCAAGG GGAAGTTTTT CTACTGCACC GACGAGTCCA AGGAGCTGGA GCGGGACTGC
    AGGGGCCAGT ATTTGGACTA CGAGAAGGAG GAGGTGGAAG CTCAGCCACG GCAGTGGAAG
    AAATACGACT TCCACTATGA CAACGTGCTG TGGGCCCTGC TAACGCTGTT CACTGTGTCC
    ACGGGCGAAG GGTGGCCCAT GGTGCTGAAA CACTCGGTGG ATGCCACCTA CGAGGAGCAG
    GGCCCCAGCC CCGGGTTCCG CATGGAGCTG TCCATCTTCT ATGTGGTCTA CTTCGTGGTC
    TTCCCCTTCT TCTTTGTGAA CATCTTTGTG GCCTTGATCA TCATCACCTT CCAGGAGCAG
    GGGGACAAAG TCATGTCTGA GTGCAGCTTG GAGAAGAACG AGAGGGCTTG TATCGACTTC
    GCCATTAGCG CCAAACCCCT GACGCGGTAC ATGCCCCAGA ACAAGCAGTC GTTCCAGTAC
    AAGACGTGGA CGTTCGTGGT CTCACCACCC TTTGAGTATT TCATCATGGC CATGATAGCC
    CTCAACACAG TGGTGCTGAT GATGAAGTTC TACGATGCGC CCTACGAGTA CGAACTGATG
    TTGAAGTGTC TGAACATCGT GTTCACGTCC ATGTTCTCCA TGGAGTGTGT GCTCAAGGTC
    ATCGCCTTTG GAGTGCTGAA CTATTTCAGA GACGCCTGGA ATGTCTTTGA CTTTGTCACT
    GTGTTGGGAA GTATTACTGA TATTTTAGTA ACAGAGATTG CGGAAACGAA CAACTTCATC
    AACCTCAGCT TCCTCCGCCT CTTCCGTGCT GCCCGACTCA TCAAGCTGCT CCGCCAAGGC
    TACACCATCC GCATTCTGCT CTGGACCTTC GTCCAGTCCT TCAAGGCCCT GCCCTATGTG
    TGCCTGCTCA TCGCCATGCT GTTCTTCATC TACGCCATCA TCGGCATGCA GGTGTTTGGA
    AACATTGCTC TGGATGACGA CACCAGCATC AACCGTCACA ATAACTTTCG GACATTTCTT
    CAGGCCTTGA TGCTGCTCTT CAGGAGTGCC ACCGGGGAGG CCTGGCATGA GATCATGCTG
    TCCTGCCTCA GCAGTCGGGC CTGTGATGAG CTCGCCAATG CCACGGAGTG TGGGAGTGAC
    TTCGCCTACT TCTACTTTGT CTCCTTCATC TTCCTTTGCT CCTTTCTGAT GTTGAACCTC
    TTTGTGGCTG TGATCATGGA CAATTTTGAG TACCTTACGC GGGACTCTTC CATCCTAGGG
    CCTCACCACC TGGACGAATT CATCCGGGTC TGGGCCGAGT ACGACCCGGC TGCGTGTGGG
    CGCATCAGTT ACAATGACAT GTTTGAGATG CTGAAACACA TGTCCCCGCC CCTGGGGCTG
    GGGAAGAAAT GCCCTGCTCG TGTTGCGTAC AAGCGCCTGG TTCGTATGAA CATGCCCATC
    TCCAATGACG ACATGACCGT CCACTTCACA TCCACGCTGA TGGCCCTCAT CCGGACTGCA
    TTGGAGATCA AGCTGGCTCC AGCTGGCACG AAGCAGCATC AGTGTGACGC AGAGCTGAGG
    AAGGAGATCT CCTCCGTGTG GGCCAATCTG CCCCAGAAGA CCCTGGACTT ACTGGTGCCA
    CCCCATAAAC CCGACGAGAT GACGGTGGGG AAGGTGTACG CAGCTCTGAT GATATTCGAC
    TTCTACAAAC AGAACAAAAC CACCAGAGAC CAGATTCACC AAGCTCCTGG AGGCCTGGCC
    CAGATGGGCC CCGTGTCCCT GTTCCACCCC CTGAAGGCCA CGCTGGAGCA GACTCAGCCG
    GCTGTGCTCC GAGGAGCCCG CGTTTTCCTT CGGCAGAAGA GCTCGGCCTC CCTCAGCAAC
    GGTGGGGCAG TACAAACCCA AGAGGGCGGC ATCAAGGAGT CTGTCTCCTG GGGCACTCAG
    AGGACCCAGG AGGTCCCCTG CGAGGTGAGG ACACCCCTGG AGCGTGGCCA CTCCACAGAG
    ATCCCGGTGC AGACAGGAAA ACCGGCTGTG GACGTCCAGA TGCAGAGCAT GGTCCTGAGA
    GGCCCTGACG GGGAGCCCCA ACCTGGGCTG GAGAGCCAGG GCCGAGCAGC TTCCATGCCC
    CGCCTGGCAG CAGAGACTCA GCCACCCCCA GATGCCAGCC CCATGAAGCG CTCCATCTCC
    ACACTGGCAC CCCAGAGGCC CCATGTGGCT CATCTGTGCA CCGCGGCCCT GGACCGCGCT
    CCAGCCAGCC AGGCCGCCCC CCCCCACCAC CACCGCTGCC ATCGCCGCAG GGACCGGAAG
    CAGAAGTCCC TGGAGAAGGG GCCCGGCCTG TCTGCAGACA CAGATGGCGC GCCCTACAGC
    ACCGCGGGGC CGGGCCCCCC GCCGCCCCCA GGGGATGGGG CAGCAGGCTG CCGGCGGGAG
    CGCAGGCAGG AGCGGGGCCG GTCCCAGGAG CGGAGGCAGC CCTCCTCCTC TTCCTCTGAG
    AAGCAGCGCT TCTACTCCTG TGACCGCTTT GGGGGCCGTG AGCCCCCGCA GCCCAAGCCC
    TCTCTCAGCA GCCATCCCAC GTCGCCCACG GCCGGGCAGG AGCCAGGACC CCCGAGACAG
    GGTAGCGGCT CCGTGAACGG GAGCCCCCTG CTGTCGACAT CTGGTGCTAG CACCCCTGGC
    CGCGGCGGGC GGAGGCAGCT CCCCCAGACC CCGCTGACCC CCCGTCCCAG CATCACCTAT
    AAGACGGCCA ACTCCTCACC TGTCCACTTT GCCGGGGCTC AGACCAGCCT CCCTGCCTTT
    TCCCCCGGCC GGCTTAGCCG TGGCCTTTCG GAACACAACG CCCTGCTCCA GAGAGACCCC
    CTCAGCCAGC CGCTGGCCCC CAGCTCTCGG ATTGGCTCCG ACCCTTACCT GGGGCAGCGT
    CTGGACAGTG AGGCTGCGGC CCGCACCCAG CTTGAGGACA CGCTCACCTT CGAGGAGGCC
    GTGGCCACCA ACTCGGGCCG CTCCTCCCGG ACTTCCTACG TGTCCTCCCT GACCTCGCAG
    TCCCACCCCC TCCGCCGGGT GCCCAACGGC TACCACTGCA CTCTGGGGCT CAGCTCGGGC
    GGGGGCGGCC GGGGGCGGCA CAGCTACCAC CACCCTGACC AAGACCACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220139
    Clone ID Related Accession (Same CDS sequence) XM_024998336.1
    Accession Version XM_024998336.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC
    ACCAAGAAGA GCCGAAACGA CCTGATCCAC GCGGAGGAGG GAGAGGACCG CTTTGCAGAC
    CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGCCTCA AGAGTGGGAA GACGGAGAGC
    TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG
    GCGCAGAGCT TCTACTGGGT GGTGCTGTGT GTGGTGGCCC TGAACACGCT GTGCGTGGCC
    ATGGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCGC TGTACTTTGC AGAGTTTGTT
    TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGGAGC
    TACTTCCGGT CCTCCTTCAA CTGCTTCGAT TTCGGGGTCA TTGTGGGGAG CATTTTCGAA
    GTGGTCTGGG CGGCCATCAA GCCAGGAACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG
    CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG
    TCCCTGCTCA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC
    GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT CCAGGATGAG
    ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG
    ACCGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AATCACAAGG TGGAGTCAGC
    AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG
    CTGAACGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG
    GACGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA
    GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCTGCCAG GCAGCAGAAC
    TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG
    CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGCCT GCGCTTCGCC
    ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG
    CCAGGCCGGG ATGGCGCGCG GGGGCCTGCG GGGGGCAAGG CCCGGCCCGA GGGCGGGGAG
    GCCGGCGAGG GTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACAGGGAC
    AAGGCCGCAG CCCCGGCGGG GGAGCCAGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG
    GAGCCGGGGG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA
    GGCCCCCGCG AGGCCAGGAG CGAGCGAGGC CGCGGGCTGG GCCCGGACGG CGGCCGGCGC
    CACCACCGGC GCGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG
    CACCGGCACG CGCCGGAGCC GAGCAGGGAG GGCGCGCCCG GGAGCAAGGG CGAGCGGCGA
    GCGCGGCACC GCGGCGGCTC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCC
    TCCCGGCGGC ACCGAGCGCG ACACAAGGCG CCGCCCGCAC ACGAGGAGGC GGAGAAGGAG
    GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGAAACCACC AGCCCAGGGA GTCGCACTGT
    GACCTGGAGG CCAGTGTGGT CATTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACTTGT
    CTAGAGAAGG TGGAAGAGCA GCCAGAGGAT GCAGACAATC AGCGGAATGT GACTCGCATG
    GGCAGTCAGC CCTCAGATCT GAGCACCGCT GTGCGTATCC CAGTGACGCT GACCGGGCCT
    CCCGGGGAGA CCACGGTTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA
    AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGTGGCC CCCGACCCAT TGTCCCGTAC
    AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTCCGCC GCTTCTGCCA CTACATTGTG
    ACCATGCGGT ACTTCGAGAT GGTCATTCTC GTGGTCATTG CCCTGAGTAG CATTGCCCTG
    GCTGCGGAGG ATCCTGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC
    TACATCTTCA CAGGTGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA TTTGGGACTG
    CTGCTGCACC CCGGTGCCTA CTTCCGGGAT CTGTGGAACA TTCTGGACTT CATTGTGGTC
    AGTGGGGCCC TGGTGGCGTT TGCCTTCTCG AGCTTCATGG GAGGCTCCAA AGGGAAAGAC
    ATCAGCACCA TCAAGTCCCT GAGGGTCCTG CGTGTGCTGA GGCCACTCAA GACCATCAAG
    CGGCTGCCCA AGCTCAAAGC TGTGTTTGAC TGTGTGGTGA ACTCCCTGAA GAACGTCCTC
    AACATCCTGA TCGTCTACAT GCTCTTCATG TTCATATTTG CTGTCATCGC GGTACAGCTC
    TTCAAGGGGA AGTTTTTCTA CTGCACCGAC GAGTCCAAGG AGCTGGAGCG GGACTGCAGG
    GGCCAGTATT TGGACTACGA GAAGGAGGAG GTGGAAGCTC AGCCACGGCA GTGGAAGAAA
    TACGACTTCC ACTATGACAA CGTGCTGTGG GCCCTGCTAA CGCTGTTCAC TGTGTCCACG
    GGCGAAGGGT GGCCCATGGT GCTGAAACAC TCGGTGGATG CCACCTACGA GGAGCAGGGC
    CCCAGCCCCG GGTTCCGCAT GGAGCTGTCC ATCTTCTATG TGGTCTACTT CGTGGTCTTC
    CCCTTCTTCT TTGTGAACAT CTTTGTGGCC TTGATCATCA TCACCTTCCA GGAGCAGGGG
    GACAAAGTCA TGTCTGAGTG CAGCTTGGAG AAGAACGAGA GGGCTTGTAT CGACTTCGCC
    ATTAGCGCCA AACCCCTGAC GCGGTACATG CCCCAGAACA AGCAGTCGTT CCAGTACAAG
    ACGTGGACGT TCGTGGTCTC ACCACCCTTT GAGTATTTCA TCATGGCCAT GATAGCCCTC
    AACACAGTGG TGCTGATGAT GAAGTTCTAC GATGCGCCCT ACGAGTACGA ACTGATGTTG
    AAGTGTCTGA ACATCGTGTT CACGTCCATG TTCTCCATGG AGTGTGTGCT CAAGGTCATC
    GCCTTTGGAG TGCTGAACTA TTTCAGAGAC GCCTGGAATG TCTTTGACTT TGTCACTGTG
    TTGGGAAGTA TTACTGATAT TTTAGTAACA GAGATTGCGG AAACGAACAA CTTCATCAAC
    CTCAGCTTCC TCCGCCTCTT CCGTGCTGCC CGACTCATCA AGCTGCTCCG CCAAGGCTAC
    ACCATCCGCA TTCTGCTCTG GACCTTCGTC CAGTCCTTCA AGGCCCTGCC CTATGTGTGC
    CTGCTCATCG CCATGCTGTT CTTCATCTAC GCCATCATCG GCATGCAGGT GTTTGGAAAC
    ATTGCTCTGG ATGACGACAC CAGCATCAAC CGTCACAATA ACTTTCGGAC ATTTCTTCAG
    GCCTTGATGC TGCTCTTCAG GAGTGCCACC GGGGAGGCCT GGCATGAGAT CATGCTGTCC
    TGCCTCAGCA GTCGGGCCTG TGATGAGCTC GCCAATGCCA CGGAGTGTGG GAGTGACTTC
    GCCTACTTCT ACTTTGTCTC CTTCATCTTC CTTTGCTCCT TTCTGATGTT GAACCTCTTT
    GTGGCTGTGA TCATGGACAA TTTTGAGTAC CTTACGCGGG ACTCTTCCAT CCTAGGGCCT
    CACCACCTGG ACGAATTCAT CCGGGTCTGG GCCGAGTACG ACCCGGCTGC GTGTGGGCGC
    ATCAGTTACA ATGACATGTT TGAGATGCTG AAACACATGT CCCCGCCCCT GGGGCTGGGG
    AAGAAATGCC CTGCTCGTGT TGCGTACAAG CGCCTGGTTC GTATGAACAT GCCCATCTCC
    AATGACGACA TGACCGTCCA CTTCACATCC ACGCTGATGG CCCTCATCCG GACTGCATTG
    GAGATCAAGC TGGCTCCAGC TGGCACGAAG CAGCATCAGT GTGACGCAGA GCTGAGGAAG
    GAGATCTCCT CCGTGTGGGC CAATCTGCCC CAGAAGACCC TGGACTTACT GGTGCCACCC
    CATAAACCCG ACGAGATGAC GGTGGGGAAG GTGTACGCAG CTCTGATGAT ATTCGACTTC
    TACAAACAGA ACAAAACCAC CAGAGACCAG ATTCACCAAG CTCCTGGAGG CCTGGCCCAG
    ATGGGCCCCG TGTCCCTGTT CCACCCCCTG AAGGCCACGC TGGAGCAGAC TCAGCCGGCT
    GTGCTCCGAG GAGCCCGCGT TTTCCTTCGG CAGAAGAGCT CGGCCTCCCT CAGCAACGGT
    GGGGCAGTAC AAACCCAAGA GGGCGGCATC AAGGAGTCTG TCTCCTGGGG CACTCAGAGG
    ACCCAGGAGG TCCCCTGCGA GGTGAGGACA CCCCTGGAGC GTGGCCACTC CACAGAGATC
    CCGGTGCAGA CAGGAAAACC GGCTGTGGAC GTCCAGATGC AGAGCATGGT CCTGAGAGGC
    CCTGACGGGG AGCCCCAACC TGGGCTGGAG AGCCAGGGCC GAGCAGCTTC CATGCCCCGC
    CTGGCAGCAG AGACTCAGCC ACCCCCAGAT GCCAGCCCCA TGAAGCGCTC CATCTCCACA
    CTGGCACCCC AGAGGCCCCA TGTGGCTCAT CTGTGCACCG CGGCCCTGGA CCGCGCTCCA
    GCCAGCCAGG CCGCCCCCCC CCACCACCAC CGCTGCCATC GCCGCAGGGA CCGGAAGCAG
    AAGTCCCTGG AGAAGGGGCC CGGCCTGTCT GCAGACACAG ATGGCGCGCC CTACAGCACC
    GCGGGGCCGG GCCCCCCGCC GCCCCCAGGG GATGGGGCAG CAGGCTGCCG GCGGGAGCGC
    AGGCAGGAGC GGGGCCGGTC CCAGGAGCGG AGGCAGCCCT CCTCCTCTTC CTCTGAGAAG
    CAGCGCTTCT ACTCCTGTGA CCGCTTTGGG GGCCGTGAGC CCCCGCAGCC CAAGCCCTCT
    CTCAGCAGCC ATCCCACGTC GCCCACGGCC GGGCAGGAGC CAGGACCCCC GAGACAGGGT
    AGCGGCTCCG TGAACGGGAG CCCCCTGCTG TCGACATCTG GTGCTAGCAC CCCTGGCCGC
    GGCGGGCGGA GGCAGCTCCC CCAGACCCCG CTGACCCCCC GTCCCAGCAT CACCTATAAG
    ACGGCCAACT CCTCACCTGT CCACTTTGCC GGGGCTCAGA CCAGCCTCCC TGCCTTTTCC
    CCCGGCCGGC TTAGCCGTGG CCTTTCGGAA CACAACGCCC TGCTCCAGAG AGACCCCCTC
    AGCCAGCCGC TGGCCCCCAG CTCTCGGATT GGCTCCGACC CTTACCTGGG GCAGCGTCTG
    GACAGTGAGG CTGCGGCCCG CACCCAGCTT GAGGACACGC TCACCTTCGA GGAGGCCGTG
    GCCACCAACT CGGGCCGCTC CTCCCGGACT TCCTACGTGT CCTCCCTGAC CTCGCAGTCC
    CACCCCCTCC GCCGGGTGCC CAACGGCTAC CACTGCACTC TGGGGCTCAG CTCGGGCGGG
    GGCGGCCGGG GGCGGCACAG CTACCACCAC CCTGACCAAG ACCACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854104.1
    CDS140..7153
    Translation

    Target ORF information:

    RefSeq Version XM_024998336.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998336.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC
    ACCAAGAAGA GCCGAAACGA CCTGATCCAC GCGGAGGAGG GAGAGGACCG CTTTGCAGAC
    CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGCCTCA AGAGTGGGAA GACGGAGAGC
    TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG
    GCGCAGAGCT TCTACTGGGT GGTGCTGTGT GTGGTGGCCC TGAACACGCT GTGCGTGGCC
    ATGGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCGC TGTACTTTGC AGAGTTTGTT
    TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGGAGC
    TACTTCCGGT CCTCCTTCAA CTGCTTCGAT TTCGGGGTCA TTGTGGGGAG CATTTTCGAA
    GTGGTCTGGG CGGCCATCAA GCCAGGAACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG
    CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG
    TCCCTGCTCA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC
    GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT CCAGGATGAG
    ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG
    ACCGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AATCACAAGG TGGAGTCAGC
    AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG
    CTGAACGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG
    GACGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA
    GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCTGCCAG GCAGCAGAAC
    TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG
    CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGCCT GCGCTTCGCC
    ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG
    CCAGGCCGGG ATGGCGCGCG GGGGCCTGCG GGGGGCAAGG CCCGGCCCGA GGGCGGGGAG
    GCCGGCGAGG GTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACAGGGAC
    AAGGCCGCAG CCCCGGCGGG GGAGCCAGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG
    GAGCCGGGGG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA
    GGCCCCCGCG AGGCCAGGAG CGAGCGAGGC CGCGGGCTGG GCCCGGACGG CGGCCGGCGC
    CACCACCGGC GCGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG
    CACCGGCACG CGCCGGAGCC GAGCAGGGAG GGCGCGCCCG GGAGCAAGGG CGAGCGGCGA
    GCGCGGCACC GCGGCGGCTC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCC
    TCCCGGCGGC ACCGAGCGCG ACACAAGGCG CCGCCCGCAC ACGAGGAGGC GGAGAAGGAG
    GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGAAACCACC AGCCCAGGGA GTCGCACTGT
    GACCTGGAGG CCAGTGTGGT CATTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACTTGT
    CTAGAGAAGG TGGAAGAGCA GCCAGAGGAT GCAGACAATC AGCGGAATGT GACTCGCATG
    GGCAGTCAGC CCTCAGATCT GAGCACCGCT GTGCGTATCC CAGTGACGCT GACCGGGCCT
    CCCGGGGAGA CCACGGTTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA
    AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGTGGCC CCCGACCCAT TGTCCCGTAC
    AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTCCGCC GCTTCTGCCA CTACATTGTG
    ACCATGCGGT ACTTCGAGAT GGTCATTCTC GTGGTCATTG CCCTGAGTAG CATTGCCCTG
    GCTGCGGAGG ATCCTGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC
    TACATCTTCA CAGGTGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA TTTGGGACTG
    CTGCTGCACC CCGGTGCCTA CTTCCGGGAT CTGTGGAACA TTCTGGACTT CATTGTGGTC
    AGTGGGGCCC TGGTGGCGTT TGCCTTCTCG AGCTTCATGG GAGGCTCCAA AGGGAAAGAC
    ATCAGCACCA TCAAGTCCCT GAGGGTCCTG CGTGTGCTGA GGCCACTCAA GACCATCAAG
    CGGCTGCCCA AGCTCAAAGC TGTGTTTGAC TGTGTGGTGA ACTCCCTGAA GAACGTCCTC
    AACATCCTGA TCGTCTACAT GCTCTTCATG TTCATATTTG CTGTCATCGC GGTACAGCTC
    TTCAAGGGGA AGTTTTTCTA CTGCACCGAC GAGTCCAAGG AGCTGGAGCG GGACTGCAGG
    GGCCAGTATT TGGACTACGA GAAGGAGGAG GTGGAAGCTC AGCCACGGCA GTGGAAGAAA
    TACGACTTCC ACTATGACAA CGTGCTGTGG GCCCTGCTAA CGCTGTTCAC TGTGTCCACG
    GGCGAAGGGT GGCCCATGGT GCTGAAACAC TCGGTGGATG CCACCTACGA GGAGCAGGGC
    CCCAGCCCCG GGTTCCGCAT GGAGCTGTCC ATCTTCTATG TGGTCTACTT CGTGGTCTTC
    CCCTTCTTCT TTGTGAACAT CTTTGTGGCC TTGATCATCA TCACCTTCCA GGAGCAGGGG
    GACAAAGTCA TGTCTGAGTG CAGCTTGGAG AAGAACGAGA GGGCTTGTAT CGACTTCGCC
    ATTAGCGCCA AACCCCTGAC GCGGTACATG CCCCAGAACA AGCAGTCGTT CCAGTACAAG
    ACGTGGACGT TCGTGGTCTC ACCACCCTTT GAGTATTTCA TCATGGCCAT GATAGCCCTC
    AACACAGTGG TGCTGATGAT GAAGTTCTAC GATGCGCCCT ACGAGTACGA ACTGATGTTG
    AAGTGTCTGA ACATCGTGTT CACGTCCATG TTCTCCATGG AGTGTGTGCT CAAGGTCATC
    GCCTTTGGAG TGCTGAACTA TTTCAGAGAC GCCTGGAATG TCTTTGACTT TGTCACTGTG
    TTGGGAAGTA TTACTGATAT TTTAGTAACA GAGATTGCGG AAACGAACAA CTTCATCAAC
    CTCAGCTTCC TCCGCCTCTT CCGTGCTGCC CGACTCATCA AGCTGCTCCG CCAAGGCTAC
    ACCATCCGCA TTCTGCTCTG GACCTTCGTC CAGTCCTTCA AGGCCCTGCC CTATGTGTGC
    CTGCTCATCG CCATGCTGTT CTTCATCTAC GCCATCATCG GCATGCAGGT GTTTGGAAAC
    ATTGCTCTGG ATGACGACAC CAGCATCAAC CGTCACAATA ACTTTCGGAC ATTTCTTCAG
    GCCTTGATGC TGCTCTTCAG GAGTGCCACC GGGGAGGCCT GGCATGAGAT CATGCTGTCC
    TGCCTCAGCA GTCGGGCCTG TGATGAGCTC GCCAATGCCA CGGAGTGTGG GAGTGACTTC
    GCCTACTTCT ACTTTGTCTC CTTCATCTTC CTTTGCTCCT TTCTGATGTT GAACCTCTTT
    GTGGCTGTGA TCATGGACAA TTTTGAGTAC CTTACGCGGG ACTCTTCCAT CCTAGGGCCT
    CACCACCTGG ACGAATTCAT CCGGGTCTGG GCCGAGTACG ACCCGGCTGC GTGTGGGCGC
    ATCAGTTACA ATGACATGTT TGAGATGCTG AAACACATGT CCCCGCCCCT GGGGCTGGGG
    AAGAAATGCC CTGCTCGTGT TGCGTACAAG CGCCTGGTTC GTATGAACAT GCCCATCTCC
    AATGACGACA TGACCGTCCA CTTCACATCC ACGCTGATGG CCCTCATCCG GACTGCATTG
    GAGATCAAGC TGGCTCCAGC TGGCACGAAG CAGCATCAGT GTGACGCAGA GCTGAGGAAG
    GAGATCTCCT CCGTGTGGGC CAATCTGCCC CAGAAGACCC TGGACTTACT GGTGCCACCC
    CATAAACCCG ACGAGATGAC GGTGGGGAAG GTGTACGCAG CTCTGATGAT ATTCGACTTC
    TACAAACAGA ACAAAACCAC CAGAGACCAG ATTCACCAAG CTCCTGGAGG CCTGGCCCAG
    ATGGGCCCCG TGTCCCTGTT CCACCCCCTG AAGGCCACGC TGGAGCAGAC TCAGCCGGCT
    GTGCTCCGAG GAGCCCGCGT TTTCCTTCGG CAGAAGAGCT CGGCCTCCCT CAGCAACGGT
    GGGGCAGTAC AAACCCAAGA GGGCGGCATC AAGGAGTCTG TCTCCTGGGG CACTCAGAGG
    ACCCAGGAGG TCCCCTGCGA GGTGAGGACA CCCCTGGAGC GTGGCCACTC CACAGAGATC
    CCGGTGCAGA CAGGAAAACC GGCTGTGGAC GTCCAGATGC AGAGCATGGT CCTGAGAGGC
    CCTGACGGGG AGCCCCAACC TGGGCTGGAG AGCCAGGGCC GAGCAGCTTC CATGCCCCGC
    CTGGCAGCAG AGACTCAGCC ACCCCCAGAT GCCAGCCCCA TGAAGCGCTC CATCTCCACA
    CTGGCACCCC AGAGGCCCCA TGTGGCTCAT CTGTGCACCG CGGCCCTGGA CCGCGCTCCA
    GCCAGCCAGG CCGCCCCCCC CCACCACCAC CGCTGCCATC GCCGCAGGGA CCGGAAGCAG
    AAGTCCCTGG AGAAGGGGCC CGGCCTGTCT GCAGACACAG ATGGCGCGCC CTACAGCACC
    GCGGGGCCGG GCCCCCCGCC GCCCCCAGGG GATGGGGCAG CAGGCTGCCG GCGGGAGCGC
    AGGCAGGAGC GGGGCCGGTC CCAGGAGCGG AGGCAGCCCT CCTCCTCTTC CTCTGAGAAG
    CAGCGCTTCT ACTCCTGTGA CCGCTTTGGG GGCCGTGAGC CCCCGCAGCC CAAGCCCTCT
    CTCAGCAGCC ATCCCACGTC GCCCACGGCC GGGCAGGAGC CAGGACCCCC GAGACAGGGT
    AGCGGCTCCG TGAACGGGAG CCCCCTGCTG TCGACATCTG GTGCTAGCAC CCCTGGCCGC
    GGCGGGCGGA GGCAGCTCCC CCAGACCCCG CTGACCCCCC GTCCCAGCAT CACCTATAAG
    ACGGCCAACT CCTCACCTGT CCACTTTGCC GGGGCTCAGA CCAGCCTCCC TGCCTTTTCC
    CCCGGCCGGC TTAGCCGTGG CCTTTCGGAA CACAACGCCC TGCTCCAGAG AGACCCCCTC
    AGCCAGCCGC TGGCCCCCAG CTCTCGGATT GGCTCCGACC CTTACCTGGG GCAGCGTCTG
    GACAGTGAGG CTGCGGCCCG CACCCAGCTT GAGGACACGC TCACCTTCGA GGAGGCCGTG
    GCCACCAACT CGGGCCGCTC CTCCCGGACT TCCTACGTGT CCTCCCTGAC CTCGCAGTCC
    CACCCCCTCC GCCGGGTGCC CAACGGCTAC CACTGCACTC TGGGGCTCAG CTCGGGCGGG
    GGCGGCCGGG GGCGGCACAG CTACCACCAC CCTGACCAAG ACCACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220140
    Clone ID Related Accession (Same CDS sequence) XM_024998337.1
    Accession Version XM_024998337.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGCAGTGTT GAAGAGAGCA
    GCCACCAAGA AGAGCCGAAA CGACCTGATC CACGCGGAGG AGGGAGAGGA CCGCTTTGCA
    GACCTCTGTG CTGTGGGGTC CCCCTTCGCC CGAGCCAGCC TCAAGAGTGG GAAGACGGAG
    AGCTCGTCAT ACTTCCGCAG GAAGGAGAAG ATGTTCCGGT TCTTCATCCG GCGCCTGGTG
    AAGGCGCAGA GCTTCTACTG GGTGGTGCTG TGTGTGGTGG CCCTGAACAC GCTGTGCGTG
    GCCATGGTGC ACTACCAGCA GCCCCAGCGG CTCACCACGG CGCTGTACTT TGCAGAGTTT
    GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGG
    AGCTACTTCC GGTCCTCCTT CAACTGCTTC GATTTCGGGG TCATTGTGGG GAGCATTTTC
    GAAGTGGTCT GGGCGGCCAT CAAGCCAGGA ACCTCCTTCG GCATCAGTGT GCTGCGGGCG
    CTGCGGCTGC TGAGAATCTT CAAAGTCACC AAGTACTGGA GCTCCCTGCG GAACCTGGTG
    GTGTCCCTGC TCAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCCTGCT CTTCCTCTTC
    ATCGTGGTCT TCGCTCTGCT GGGCATGCAG CTCTTCGGGG GACAGTTCAA CTTCCAGGAT
    GAGACTCCGA CAACCAACTT TGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC
    CTGACCGGGG AGGACTGGAA TGCGGTGATG TACCATGGGA TCGAATCACA AGGTGGAGTC
    AGCAAGGGCA TGTTCTCGTC TGTGTACTTC ATTGTCCTGA CGCTGTTTGG GAACTACACC
    CTGCTGAACG TCTTCCTGGC CATCGCTGTG GACAACCTCG CCAATGCTCA GGAGCTGACC
    AAGGACGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA
    GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATTGCTGC CAGGCAGCAG
    AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTGCG GCTGCAGAAC
    CTGCGGGCCA GCTGTGAAGC GCTGTATAGC GAGATGGACC CCGAGGAGCG CCTGCGCTTC
    GCCACTTCCC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTTGTGGTG
    GAGCCAGGCC GGGATGGCGC GCGGGGGCCT GCGGGGGGCA AGGCCCGGCC CGAGGGCGGG
    GAGGCCGGCG AGGGTGCCGA CCCGCCGCGC AGGCACCACC GGCACCGCGA CAGGGACAGG
    GACAAGGCCG CAGCCCCGGC GGGGGAGCCA GACAGGGCGG ACGCCCCGAA GGCGGAGGGC
    GGGGAGCCGG GGGCCCGGGA GGAGCGGGCG CGACCGCGCC GCAGCCGCAG CAAGGAGGCC
    GCAGGCCCCC GCGAGGCCAG GAGCGAGCGA GGCCGCGGGC TGGGCCCGGA CGGCGGCCGG
    CGCCACCACC GGCGCGGCTC CCCGGAGGAG GCCGCGGAGC GCGAGCCCCG GCGCCACCGC
    GCGCACCGGC ACGCGCCGGA GCCGAGCAGG GAGGGCGCGC CCGGGAGCAA GGGCGAGCGG
    CGAGCGCGGC ACCGCGGCGG CTCCCGGGCC GGGCCCCGGG AGGCCGAGAG CGGCGAGGAG
    CCCTCCCGGC GGCACCGAGC GCGACACAAG GCGCCGCCCG CACACGAGGA GGCGGAGAAG
    GAGGCCGAGG CGGAGGACAG GGACAAGGAG CCCCGAAACC ACCAGCCCAG GGAGTCGCAC
    TGTGACCTGG AGGCCAGTGT GGTCATTGGT GTGGGCCCTG TGCATGCCCT GCCCAGCACT
    TGTCTAGAGA AGGTGGAAGA GCAGCCAGAG GATGCAGACA ATCAGCGGAA TGTGACTCGC
    ATGGGCAGTC AGCCCTCAGA TCTGAGCACC GCTGTGCGTA TCCCAGTGAC GCTGACCGGG
    CCTCCCGGGG AGACCACGGT TGTTCCCAGT GGTAACGTGG ACCTGGAAAG CCAAGCAGAA
    GGAAAGAAGG AGGTGGAAGC TGATGACGTG ATGAGGAGTG GCCCCCGACC CATTGTCCCG
    TACAGCTCCA TGTTCTGCTT GAGCCCCACC AACCTGCTCC GCCGCTTCTG CCACTACATT
    GTGACCATGC GGTACTTCGA GATGGTCATT CTCGTGGTCA TTGCCCTGAG TAGCATTGCC
    CTGGCTGCGG AGGATCCTGT GCAGACAGAC TCGCCCAGGA ACAACGTGCT GAAGTACATG
    GACTACATCT TCACAGGTGT CTTCACCTTT GAGATGGTGA TAAAGATGAT CGATTTGGGA
    CTGCTGCTGC ACCCCGGTGC CTACTTCCGG GATCTGTGGA ACATTCTGGA CTTCATTGTG
    GTCAGTGGGG CCCTGGTGGC GTTTGCCTTC TCGAGCTTCA TGGGAGGCTC CAAAGGGAAA
    GACATCAGCA CCATCAAGTC CCTGAGGGTC CTGCGTGTGC TGAGGCCACT CAAGACCATC
    AAGCGGCTGC CCAAGCTCAA AGCTGTGTTT GACTGTGTGG TGAACTCCCT GAAGAACGTC
    CTCAACATCC TGATCGTCTA CATGCTCTTC ATGTTCATAT TTGCTGTCAT CGCGGTACAG
    CTCTTCAAGG GGAAGTTTTT CTACTGCACC GACGAGTCCA AGGAGCTGGA GCGGGACTGC
    AGGGGCCAGT ATTTGGACTA CGAGAAGGAG GAGGTGGAAG CTCAGCCACG GCAGTGGAAG
    AAATACGACT TCCACTATGA CAACGTGCTG TGGGCCCTGC TAACGCTGTT CACTGTGTCC
    ACGGGCGAAG GGTGGCCCAT GGTGCTGAAA CACTCGGTGG ATGCCACCTA CGAGGAGCAG
    GGCCCCAGCC CCGGGTTCCG CATGGAGCTG TCCATCTTCT ATGTGGTCTA CTTCGTGGTC
    TTCCCCTTCT TCTTTGTGAA CATCTTTGTG GCCTTGATCA TCATCACCTT CCAGGAGCAG
    GGGGACAAAG TCATGTCTGA GTGCAGCTTG GAGAAGAACG AGAGGGCTTG TATCGACTTC
    GCCATTAGCG CCAAACCCCT GACGCGGTAC ATGCCCCAGA ACAAGCAGTC GTTCCAGTAC
    AAGACGTGGA CGTTCGTGGT CTCACCACCC TTTGAGTATT TCATCATGGC CATGATAGCC
    CTCAACACAG TGGTGCTGAT GATGAAGTTC TACGATGCGC CCTACGAGTA CGAACTGATG
    TTGAAGTGTC TGAACATCGT GTTCACGTCC ATGTTCTCCA TGGAGTGTGT GCTCAAGGTC
    ATCGCCTTTG GAGTGCTGAA CTATTTCAGA GACGCCTGGA ATGTCTTTGA CTTTGTCACT
    GTGTTGGGAA GTATTACTGA TATTTTAGTA ACAGAGATTG CGAACAACTT CATCAACCTC
    AGCTTCCTCC GCCTCTTCCG TGCTGCCCGA CTCATCAAGC TGCTCCGCCA AGGCTACACC
    ATCCGCATTC TGCTCTGGAC CTTCGTCCAG TCCTTCAAGG CCCTGCCCTA TGTGTGCCTG
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATCGGCA TGCAGGTGTT TGGAAACATT
    GCTCTGGATG ACGACACCAG CATCAACCGT CACAATAACT TTCGGACATT TCTTCAGGCC
    TTGATGCTGC TCTTCAGGAG TGCCACCGGG GAGGCCTGGC ATGAGATCAT GCTGTCCTGC
    CTCAGCAGTC GGGCCTGTGA TGAGCTCGCC AATGCCACGG AGTGTGGGAG TGACTTCGCC
    TACTTCTACT TTGTCTCCTT CATCTTCCTT TGCTCCTTTC TGATGTTGAA CCTCTTTGTG
    GCTGTGATCA TGGACAATTT TGAGTACCTT ACGCGGGACT CTTCCATCCT AGGGCCTCAC
    CACCTGGACG AATTCATCCG GGTCTGGGCC GAGTACGACC CGGCTGCGTG TGGGCGCATC
    AGTTACAATG ACATGTTTGA GATGCTGAAA CACATGTCCC CGCCCCTGGG GCTGGGGAAG
    AAATGCCCTG CTCGTGTTGC GTACAAGCGC CTGGTTCGTA TGAACATGCC CATCTCCAAT
    GACGACATGA CCGTCCACTT CACATCCACG CTGATGGCCC TCATCCGGAC TGCATTGGAG
    ATCAAGCTGG CTCCAGCTGG CACGAAGCAG CATCAGTGTG ACGCAGAGCT GAGGAAGGAG
    ATCTCCTCCG TGTGGGCCAA TCTGCCCCAG AAGACCCTGG ACTTACTGGT GCCACCCCAT
    AAACCCGACG AGATGACGGT GGGGAAGGTG TACGCAGCTC TGATGATATT CGACTTCTAC
    AAACAGAACA AAACCACCAG AGACCAGATT CACCAAGCTC CTGGAGGCCT GGCCCAGATG
    GGCCCCGTGT CCCTGTTCCA CCCCCTGAAG GCCACGCTGG AGCAGACTCA GCCGGCTGTG
    CTCCGAGGAG CCCGCGTTTT CCTTCGGCAG AAGAGCTCGG CCTCCCTCAG CAACGGTGGG
    GCAGTACAAA CCCAAGAGGG CGGCATCAAG GAGTCTGTCT CCTGGGGCAC TCAGAGGACC
    CAGGAGGTCC CCTGCGAGGT GAGGACACCC CTGGAGCGTG GCCACTCCAC AGAGATCCCG
    GTGCAGACAG GAAAACCGGC TGTGGACGTC CAGATGCAGA GCATGGTCCT GAGAGGCCCT
    GACGGGGAGC CCCAACCTGG GCTGGAGAGC CAGGGCCGAG CAGCTTCCAT GCCCCGCCTG
    GCAGCAGAGA CTCAGCCACC CCCAGATGCC AGCCCCATGA AGCGCTCCAT CTCCACACTG
    GCACCCCAGA GGCCCCATGT GGCTCATCTG TGCACCGCGG CCCTGGACCG CGCTCCAGCC
    AGCCAGGCCG CCCCCCCCCA CCACCACCGC TGCCATCGCC GCAGGGACCG GAAGCAGAAG
    TCCCTGGAGA AGGGGCCCGG CCTGTCTGCA GACACAGATG GCGCGCCCTA CAGCACCGCG
    GGGCCGGGCC CCCCGCCGCC CCCAGGGGAT GGGGCAGCAG GCTGCCGGCG GGAGCGCAGG
    CAGGAGCGGG GCCGGTCCCA GGAGCGGAGG CAGCCCTCCT CCTCTTCCTC TGAGAAGCAG
    CGCTTCTACT CCTGTGACCG CTTTGGGGGC CGTGAGCCCC CGCAGCCCAA GCCCTCTCTC
    AGCAGCCATC CCACGTCGCC CACGGCCGGG CAGGAGCCAG GACCCCCGAG ACAGGGTAGC
    GGCTCCGTGA ACGGGAGCCC CCTGCTGTCG ACATCTGGTG CTAGCACCCC TGGCCGCGGC
    GGGCGGAGGC AGCTCCCCCA GACCCCGCTG ACCCCCCGTC CCAGCATCAC CTATAAGACG
    GCCAACTCCT CACCTGTCCA CTTTGCCGGG GCTCAGACCA GCCTCCCTGC CTTTTCCCCC
    GGCCGGCTTA GCCGTGGCCT TTCGGAACAC AACGCCCTGC TCCAGAGAGA CCCCCTCAGC
    CAGCCGCTGG CCCCCAGCTC TCGGATTGGC TCCGACCCTT ACCTGGGGCA GCGTCTGGAC
    AGTGAGGCTG CGGCCCGCAC CCAGCTTGAG GACACGCTCA CCTTCGAGGA GGCCGTGGCC
    ACCAACTCGG GCCGCTCCTC CCGGACTTCC TACGTGTCCT CCCTGACCTC GCAGTCCCAC
    CCCCTCCGCC GGGTGCCCAA CGGCTACCAC TGCACTCTGG GGCTCAGCTC GGGCGGGGGC
    GGCCGGGGGC GGCACAGCTA CCACCACCCT GACCAAGACC ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854105.1
    CDS1..7011
    Translation

    Target ORF information:

    RefSeq Version XM_024998337.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998337.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGCAGTGTT GAAGAGAGCA
    GCCACCAAGA AGAGCCGAAA CGACCTGATC CACGCGGAGG AGGGAGAGGA CCGCTTTGCA
    GACCTCTGTG CTGTGGGGTC CCCCTTCGCC CGAGCCAGCC TCAAGAGTGG GAAGACGGAG
    AGCTCGTCAT ACTTCCGCAG GAAGGAGAAG ATGTTCCGGT TCTTCATCCG GCGCCTGGTG
    AAGGCGCAGA GCTTCTACTG GGTGGTGCTG TGTGTGGTGG CCCTGAACAC GCTGTGCGTG
    GCCATGGTGC ACTACCAGCA GCCCCAGCGG CTCACCACGG CGCTGTACTT TGCAGAGTTT
    GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGG
    AGCTACTTCC GGTCCTCCTT CAACTGCTTC GATTTCGGGG TCATTGTGGG GAGCATTTTC
    GAAGTGGTCT GGGCGGCCAT CAAGCCAGGA ACCTCCTTCG GCATCAGTGT GCTGCGGGCG
    CTGCGGCTGC TGAGAATCTT CAAAGTCACC AAGTACTGGA GCTCCCTGCG GAACCTGGTG
    GTGTCCCTGC TCAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCCTGCT CTTCCTCTTC
    ATCGTGGTCT TCGCTCTGCT GGGCATGCAG CTCTTCGGGG GACAGTTCAA CTTCCAGGAT
    GAGACTCCGA CAACCAACTT TGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC
    CTGACCGGGG AGGACTGGAA TGCGGTGATG TACCATGGGA TCGAATCACA AGGTGGAGTC
    AGCAAGGGCA TGTTCTCGTC TGTGTACTTC ATTGTCCTGA CGCTGTTTGG GAACTACACC
    CTGCTGAACG TCTTCCTGGC CATCGCTGTG GACAACCTCG CCAATGCTCA GGAGCTGACC
    AAGGACGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA
    GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATTGCTGC CAGGCAGCAG
    AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTGCG GCTGCAGAAC
    CTGCGGGCCA GCTGTGAAGC GCTGTATAGC GAGATGGACC CCGAGGAGCG CCTGCGCTTC
    GCCACTTCCC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTTGTGGTG
    GAGCCAGGCC GGGATGGCGC GCGGGGGCCT GCGGGGGGCA AGGCCCGGCC CGAGGGCGGG
    GAGGCCGGCG AGGGTGCCGA CCCGCCGCGC AGGCACCACC GGCACCGCGA CAGGGACAGG
    GACAAGGCCG CAGCCCCGGC GGGGGAGCCA GACAGGGCGG ACGCCCCGAA GGCGGAGGGC
    GGGGAGCCGG GGGCCCGGGA GGAGCGGGCG CGACCGCGCC GCAGCCGCAG CAAGGAGGCC
    GCAGGCCCCC GCGAGGCCAG GAGCGAGCGA GGCCGCGGGC TGGGCCCGGA CGGCGGCCGG
    CGCCACCACC GGCGCGGCTC CCCGGAGGAG GCCGCGGAGC GCGAGCCCCG GCGCCACCGC
    GCGCACCGGC ACGCGCCGGA GCCGAGCAGG GAGGGCGCGC CCGGGAGCAA GGGCGAGCGG
    CGAGCGCGGC ACCGCGGCGG CTCCCGGGCC GGGCCCCGGG AGGCCGAGAG CGGCGAGGAG
    CCCTCCCGGC GGCACCGAGC GCGACACAAG GCGCCGCCCG CACACGAGGA GGCGGAGAAG
    GAGGCCGAGG CGGAGGACAG GGACAAGGAG CCCCGAAACC ACCAGCCCAG GGAGTCGCAC
    TGTGACCTGG AGGCCAGTGT GGTCATTGGT GTGGGCCCTG TGCATGCCCT GCCCAGCACT
    TGTCTAGAGA AGGTGGAAGA GCAGCCAGAG GATGCAGACA ATCAGCGGAA TGTGACTCGC
    ATGGGCAGTC AGCCCTCAGA TCTGAGCACC GCTGTGCGTA TCCCAGTGAC GCTGACCGGG
    CCTCCCGGGG AGACCACGGT TGTTCCCAGT GGTAACGTGG ACCTGGAAAG CCAAGCAGAA
    GGAAAGAAGG AGGTGGAAGC TGATGACGTG ATGAGGAGTG GCCCCCGACC CATTGTCCCG
    TACAGCTCCA TGTTCTGCTT GAGCCCCACC AACCTGCTCC GCCGCTTCTG CCACTACATT
    GTGACCATGC GGTACTTCGA GATGGTCATT CTCGTGGTCA TTGCCCTGAG TAGCATTGCC
    CTGGCTGCGG AGGATCCTGT GCAGACAGAC TCGCCCAGGA ACAACGTGCT GAAGTACATG
    GACTACATCT TCACAGGTGT CTTCACCTTT GAGATGGTGA TAAAGATGAT CGATTTGGGA
    CTGCTGCTGC ACCCCGGTGC CTACTTCCGG GATCTGTGGA ACATTCTGGA CTTCATTGTG
    GTCAGTGGGG CCCTGGTGGC GTTTGCCTTC TCGAGCTTCA TGGGAGGCTC CAAAGGGAAA
    GACATCAGCA CCATCAAGTC CCTGAGGGTC CTGCGTGTGC TGAGGCCACT CAAGACCATC
    AAGCGGCTGC CCAAGCTCAA AGCTGTGTTT GACTGTGTGG TGAACTCCCT GAAGAACGTC
    CTCAACATCC TGATCGTCTA CATGCTCTTC ATGTTCATAT TTGCTGTCAT CGCGGTACAG
    CTCTTCAAGG GGAAGTTTTT CTACTGCACC GACGAGTCCA AGGAGCTGGA GCGGGACTGC
    AGGGGCCAGT ATTTGGACTA CGAGAAGGAG GAGGTGGAAG CTCAGCCACG GCAGTGGAAG
    AAATACGACT TCCACTATGA CAACGTGCTG TGGGCCCTGC TAACGCTGTT CACTGTGTCC
    ACGGGCGAAG GGTGGCCCAT GGTGCTGAAA CACTCGGTGG ATGCCACCTA CGAGGAGCAG
    GGCCCCAGCC CCGGGTTCCG CATGGAGCTG TCCATCTTCT ATGTGGTCTA CTTCGTGGTC
    TTCCCCTTCT TCTTTGTGAA CATCTTTGTG GCCTTGATCA TCATCACCTT CCAGGAGCAG
    GGGGACAAAG TCATGTCTGA GTGCAGCTTG GAGAAGAACG AGAGGGCTTG TATCGACTTC
    GCCATTAGCG CCAAACCCCT GACGCGGTAC ATGCCCCAGA ACAAGCAGTC GTTCCAGTAC
    AAGACGTGGA CGTTCGTGGT CTCACCACCC TTTGAGTATT TCATCATGGC CATGATAGCC
    CTCAACACAG TGGTGCTGAT GATGAAGTTC TACGATGCGC CCTACGAGTA CGAACTGATG
    TTGAAGTGTC TGAACATCGT GTTCACGTCC ATGTTCTCCA TGGAGTGTGT GCTCAAGGTC
    ATCGCCTTTG GAGTGCTGAA CTATTTCAGA GACGCCTGGA ATGTCTTTGA CTTTGTCACT
    GTGTTGGGAA GTATTACTGA TATTTTAGTA ACAGAGATTG CGAACAACTT CATCAACCTC
    AGCTTCCTCC GCCTCTTCCG TGCTGCCCGA CTCATCAAGC TGCTCCGCCA AGGCTACACC
    ATCCGCATTC TGCTCTGGAC CTTCGTCCAG TCCTTCAAGG CCCTGCCCTA TGTGTGCCTG
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATCGGCA TGCAGGTGTT TGGAAACATT
    GCTCTGGATG ACGACACCAG CATCAACCGT CACAATAACT TTCGGACATT TCTTCAGGCC
    TTGATGCTGC TCTTCAGGAG TGCCACCGGG GAGGCCTGGC ATGAGATCAT GCTGTCCTGC
    CTCAGCAGTC GGGCCTGTGA TGAGCTCGCC AATGCCACGG AGTGTGGGAG TGACTTCGCC
    TACTTCTACT TTGTCTCCTT CATCTTCCTT TGCTCCTTTC TGATGTTGAA CCTCTTTGTG
    GCTGTGATCA TGGACAATTT TGAGTACCTT ACGCGGGACT CTTCCATCCT AGGGCCTCAC
    CACCTGGACG AATTCATCCG GGTCTGGGCC GAGTACGACC CGGCTGCGTG TGGGCGCATC
    AGTTACAATG ACATGTTTGA GATGCTGAAA CACATGTCCC CGCCCCTGGG GCTGGGGAAG
    AAATGCCCTG CTCGTGTTGC GTACAAGCGC CTGGTTCGTA TGAACATGCC CATCTCCAAT
    GACGACATGA CCGTCCACTT CACATCCACG CTGATGGCCC TCATCCGGAC TGCATTGGAG
    ATCAAGCTGG CTCCAGCTGG CACGAAGCAG CATCAGTGTG ACGCAGAGCT GAGGAAGGAG
    ATCTCCTCCG TGTGGGCCAA TCTGCCCCAG AAGACCCTGG ACTTACTGGT GCCACCCCAT
    AAACCCGACG AGATGACGGT GGGGAAGGTG TACGCAGCTC TGATGATATT CGACTTCTAC
    AAACAGAACA AAACCACCAG AGACCAGATT CACCAAGCTC CTGGAGGCCT GGCCCAGATG
    GGCCCCGTGT CCCTGTTCCA CCCCCTGAAG GCCACGCTGG AGCAGACTCA GCCGGCTGTG
    CTCCGAGGAG CCCGCGTTTT CCTTCGGCAG AAGAGCTCGG CCTCCCTCAG CAACGGTGGG
    GCAGTACAAA CCCAAGAGGG CGGCATCAAG GAGTCTGTCT CCTGGGGCAC TCAGAGGACC
    CAGGAGGTCC CCTGCGAGGT GAGGACACCC CTGGAGCGTG GCCACTCCAC AGAGATCCCG
    GTGCAGACAG GAAAACCGGC TGTGGACGTC CAGATGCAGA GCATGGTCCT GAGAGGCCCT
    GACGGGGAGC CCCAACCTGG GCTGGAGAGC CAGGGCCGAG CAGCTTCCAT GCCCCGCCTG
    GCAGCAGAGA CTCAGCCACC CCCAGATGCC AGCCCCATGA AGCGCTCCAT CTCCACACTG
    GCACCCCAGA GGCCCCATGT GGCTCATCTG TGCACCGCGG CCCTGGACCG CGCTCCAGCC
    AGCCAGGCCG CCCCCCCCCA CCACCACCGC TGCCATCGCC GCAGGGACCG GAAGCAGAAG
    TCCCTGGAGA AGGGGCCCGG CCTGTCTGCA GACACAGATG GCGCGCCCTA CAGCACCGCG
    GGGCCGGGCC CCCCGCCGCC CCCAGGGGAT GGGGCAGCAG GCTGCCGGCG GGAGCGCAGG
    CAGGAGCGGG GCCGGTCCCA GGAGCGGAGG CAGCCCTCCT CCTCTTCCTC TGAGAAGCAG
    CGCTTCTACT CCTGTGACCG CTTTGGGGGC CGTGAGCCCC CGCAGCCCAA GCCCTCTCTC
    AGCAGCCATC CCACGTCGCC CACGGCCGGG CAGGAGCCAG GACCCCCGAG ACAGGGTAGC
    GGCTCCGTGA ACGGGAGCCC CCTGCTGTCG ACATCTGGTG CTAGCACCCC TGGCCGCGGC
    GGGCGGAGGC AGCTCCCCCA GACCCCGCTG ACCCCCCGTC CCAGCATCAC CTATAAGACG
    GCCAACTCCT CACCTGTCCA CTTTGCCGGG GCTCAGACCA GCCTCCCTGC CTTTTCCCCC
    GGCCGGCTTA GCCGTGGCCT TTCGGAACAC AACGCCCTGC TCCAGAGAGA CCCCCTCAGC
    CAGCCGCTGG CCCCCAGCTC TCGGATTGGC TCCGACCCTT ACCTGGGGCA GCGTCTGGAC
    AGTGAGGCTG CGGCCCGCAC CCAGCTTGAG GACACGCTCA CCTTCGAGGA GGCCGTGGCC
    ACCAACTCGG GCCGCTCCTC CCGGACTTCC TACGTGTCCT CCCTGACCTC GCAGTCCCAC
    CCCCTCCGCC GGGTGCCCAA CGGCTACCAC TGCACTCTGG GGCTCAGCTC GGGCGGGGGC
    GGCCGGGGGC GGCACAGCTA CCACCACCCT GACCAAGACC ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220141
    Clone ID Related Accession (Same CDS sequence) XM_024998338.1
    Accession Version XM_024998338.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC
    ACCAAGAAGA GCCGAAACGA CCTGATCCAC GCGGAGGAGG GAGAGGACCG CTTTGCAGAC
    CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGCCTCA AGAGTGGGAA GACGGAGAGC
    TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG
    GCGCAGAGCT TCTACTGGGT GGTGCTGTGT GTGGTGGCCC TGAACACGCT GTGCGTGGCC
    ATGGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCGC TGTACTTTGC AGAGTTTGTT
    TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGGAGC
    TACTTCCGGT CCTCCTTCAA CTGCTTCGAT TTCGGGGTCA TTGTGGGGAG CATTTTCGAA
    GTGGTCTGGG CGGCCATCAA GCCAGGAACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG
    CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG
    TCCCTGCTCA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC
    GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT CCAGGATGAG
    ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG
    ACCGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AATCACAAGG TGGAGTCAGC
    AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG
    CTGAACGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG
    GACGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA
    GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCTGCCAG GCAGCAGAAC
    TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG
    CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGCCT GCGCTTCGCC
    ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG
    CCAGGCCGGG ATGGCGCGCG GGGGCCTGCG GGGGGCAAGG CCCGGCCCGA GGGCGGGGAG
    GCCGGCGAGG GTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACAGGGAC
    AAGGCCGCAG CCCCGGCGGG GGAGCCAGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG
    GAGCCGGGGG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA
    GGCCCCCGCG AGGCCAGGAG CGAGCGAGGC CGCGGGCTGG GCCCGGACGG CGGCCGGCGC
    CACCACCGGC GCGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG
    CACCGGCACG CGCCGGAGCC GAGCAGGGAG GGCGCGCCCG GGAGCAAGGG CGAGCGGCGA
    GCGCGGCACC GCGGCGGCTC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCC
    TCCCGGCGGC ACCGAGCGCG ACACAAGGCG CCGCCCGCAC ACGAGGAGGC GGAGAAGGAG
    GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGAAACCACC AGCCCAGGGA GTCGCACTGT
    GACCTGGAGG CCAGTGTGGT CATTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACTTGT
    CTAGAGAAGG TGGAAGAGCA GCCAGAGGAT GCAGACAATC AGCGGAATGT GACTCGCATG
    GGCAGTCAGC CCTCAGATCT GAGCACCGCT GTGCGTATCC CAGTGACGCT GACCGGGCCT
    CCCGGGGAGA CCACGGTTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA
    AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGTGGCC CCCGACCCAT TGTCCCGTAC
    AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTCCGCC GCTTCTGCCA CTACATTGTG
    ACCATGCGGT ACTTCGAGAT GGTCATTCTC GTGGTCATTG CCCTGAGTAG CATTGCCCTG
    GCTGCGGAGG ATCCTGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC
    TACATCTTCA CAGGTGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA TTTGGGACTG
    CTGCTGCACC CCGGTGCCTA CTTCCGGGAT CTGTGGAACA TTCTGGACTT CATTGTGGTC
    AGTGGGGCCC TGGTGGCGTT TGCCTTCTCG AGCTTCATGG GAGGCTCCAA AGGGAAAGAC
    ATCAGCACCA TCAAGTCCCT GAGGGTCCTG CGTGTGCTGA GGCCACTCAA GACCATCAAG
    CGGCTGCCCA AGCTCAAAGC TGTGTTTGAC TGTGTGGTGA ACTCCCTGAA GAACGTCCTC
    AACATCCTGA TCGTCTACAT GCTCTTCATG TTCATATTTG CTGTCATCGC GGTACAGCTC
    TTCAAGGGGA AGTTTTTCTA CTGCACCGAC GAGTCCAAGG AGCTGGAGCG GGACTGCAGG
    GGCCAGTATT TGGACTACGA GAAGGAGGAG GTGGAAGCTC AGCCACGGCA GTGGAAGAAA
    TACGACTTCC ACTATGACAA CGTGCTGTGG GCCCTGCTAA CGCTGTTCAC TGTGTCCACG
    GGCGAAGGGT GGCCCATGGT GCTGAAACAC TCGGTGGATG CCACCTACGA GGAGCAGGGC
    CCCAGCCCCG GGTTCCGCAT GGAGCTGTCC ATCTTCTATG TGGTCTACTT CGTGGTCTTC
    CCCTTCTTCT TTGTGAACAT CTTTGTGGCC TTGATCATCA TCACCTTCCA GGAGCAGGGG
    GACAAAGTCA TGTCTGAGTG CAGCTTGGAG AAGAACGAGA GGGCTTGTAT CGACTTCGCC
    ATTAGCGCCA AACCCCTGAC GCGGTACATG CCCCAGAACA AGCAGTCGTT CCAGTACAAG
    ACGTGGACGT TCGTGGTCTC ACCACCCTTT GAGTATTTCA TCATGGCCAT GATAGCCCTC
    AACACAGTGG TGCTGATGAT GAAGTTCTAC GATGCGCCCT ACGAGTACGA ACTGATGTTG
    AAGTGTCTGA ACATCGTGTT CACGTCCATG TTCTCCATGG AGTGTGTGCT CAAGGTCATC
    GCCTTTGGAG TGCTGAACTA TTTCAGAGAC GCCTGGAATG TCTTTGACTT TGTCACTGTG
    TTGGGAAGTA TTACTGATAT TTTAGTAACA GAGATTGCGA ACAACTTCAT CAACCTCAGC
    TTCCTCCGCC TCTTCCGTGC TGCCCGACTC ATCAAGCTGC TCCGCCAAGG CTACACCATC
    CGCATTCTGC TCTGGACCTT CGTCCAGTCC TTCAAGGCCC TGCCCTATGT GTGCCTGCTC
    ATCGCCATGC TGTTCTTCAT CTACGCCATC ATCGGCATGC AGGTGTTTGG AAACATTGCT
    CTGGATGACG ACACCAGCAT CAACCGTCAC AATAACTTTC GGACATTTCT TCAGGCCTTG
    ATGCTGCTCT TCAGGAGTGC CACCGGGGAG GCCTGGCATG AGATCATGCT GTCCTGCCTC
    AGCAGTCGGG CCTGTGATGA GCTCGCCAAT GCCACGGAGT GTGGGAGTGA CTTCGCCTAC
    TTCTACTTTG TCTCCTTCAT CTTCCTTTGC TCCTTTCTGA TGTTGAACCT CTTTGTGGCT
    GTGATCATGG ACAATTTTGA GTACCTTACG CGGGACTCTT CCATCCTAGG GCCTCACCAC
    CTGGACGAAT TCATCCGGGT CTGGGCCGAG TACGACCCGG CTGCGTGTGG GCGCATCAGT
    TACAATGACA TGTTTGAGAT GCTGAAACAC ATGTCCCCGC CCCTGGGGCT GGGGAAGAAA
    TGCCCTGCTC GTGTTGCGTA CAAGCGCCTG GTTCGTATGA ACATGCCCAT CTCCAATGAC
    GACATGACCG TCCACTTCAC ATCCACGCTG ATGGCCCTCA TCCGGACTGC ATTGGAGATC
    AAGCTGGCTC CAGCTGGCAC GAAGCAGCAT CAGTGTGACG CAGAGCTGAG GAAGGAGATC
    TCCTCCGTGT GGGCCAATCT GCCCCAGAAG ACCCTGGACT TACTGGTGCC ACCCCATAAA
    CCCGACGAGA TGACGGTGGG GAAGGTGTAC GCAGCTCTGA TGATATTCGA CTTCTACAAA
    CAGAACAAAA CCACCAGAGA CCAGATTCAC CAAGCTCCTG GAGGCCTGGC CCAGATGGGC
    CCCGTGTCCC TGTTCCACCC CCTGAAGGCC ACGCTGGAGC AGACTCAGCC GGCTGTGCTC
    CGAGGAGCCC GCGTTTTCCT TCGGCAGAAG AGCTCGGCCT CCCTCAGCAA CGGTGGGGCA
    GTACAAACCC AAGAGGGCGG CATCAAGGAG TCTGTCTCCT GGGGCACTCA GAGGACCCAG
    GAGGTCCCCT GCGAGGTGAG GACACCCCTG GAGCGTGGCC ACTCCACAGA GATCCCGGTG
    CAGACAGGAA AACCGGCTGT GGACGTCCAG ATGCAGAGCA TGGTCCTGAG AGGCCCTGAC
    GGGGAGCCCC AACCTGGGCT GGAGAGCCAG GGCCGAGCAG CTTCCATGCC CCGCCTGGCA
    GCAGAGACTC AGCCACCCCC AGATGCCAGC CCCATGAAGC GCTCCATCTC CACACTGGCA
    CCCCAGAGGC CCCATGTGGC TCATCTGTGC ACCGCGGCCC TGGACCGCGC TCCAGCCAGC
    CAGGCCGCCC CCCCCCACCA CCACCGCTGC CATCGCCGCA GGGACCGGAA GCAGAAGTCC
    CTGGAGAAGG GGCCCGGCCT GTCTGCAGAC ACAGATGGCG CGCCCTACAG CACCGCGGGG
    CCGGGCCCCC CGCCGCCCCC AGGGGATGGG GCAGCAGGCT GCCGGCGGGA GCGCAGGCAG
    GAGCGGGGCC GGTCCCAGGA GCGGAGGCAG CCCTCCTCCT CTTCCTCTGA GAAGCAGCGC
    TTCTACTCCT GTGACCGCTT TGGGGGCCGT GAGCCCCCGC AGCCCAAGCC CTCTCTCAGC
    AGCCATCCCA CGTCGCCCAC GGCCGGGCAG GAGCCAGGAC CCCCGAGACA GGGTAGCGGC
    TCCGTGAACG GGAGCCCCCT GCTGTCGACA TCTGGTGCTA GCACCCCTGG CCGCGGCGGG
    CGGAGGCAGC TCCCCCAGAC CCCGCTGACC CCCCGTCCCA GCATCACCTA TAAGACGGCC
    AACTCCTCAC CTGTCCACTT TGCCGGGGCT CAGACCAGCC TCCCTGCCTT TTCCCCCGGC
    CGGCTTAGCC GTGGCCTTTC GGAACACAAC GCCCTGCTCC AGAGAGACCC CCTCAGCCAG
    CCGCTGGCCC CCAGCTCTCG GATTGGCTCC GACCCTTACC TGGGGCAGCG TCTGGACAGT
    GAGGCTGCGG CCCGCACCCA GCTTGAGGAC ACGCTCACCT TCGAGGAGGC CGTGGCCACC
    AACTCGGGCC GCTCCTCCCG GACTTCCTAC GTGTCCTCCC TGACCTCGCA GTCCCACCCC
    CTCCGCCGGG TGCCCAACGG CTACCACTGC ACTCTGGGGC TCAGCTCGGG CGGGGGCGGC
    CGGGGGCGGC ACAGCTACCA CCACCCTGAC CAAGACCACT GGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854106.1
    CDS1..7008
    Translation

    Target ORF information:

    RefSeq Version XM_024998338.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998338.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC
    ACCAAGAAGA GCCGAAACGA CCTGATCCAC GCGGAGGAGG GAGAGGACCG CTTTGCAGAC
    CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGCCTCA AGAGTGGGAA GACGGAGAGC
    TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG
    GCGCAGAGCT TCTACTGGGT GGTGCTGTGT GTGGTGGCCC TGAACACGCT GTGCGTGGCC
    ATGGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCGC TGTACTTTGC AGAGTTTGTT
    TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGGAGC
    TACTTCCGGT CCTCCTTCAA CTGCTTCGAT TTCGGGGTCA TTGTGGGGAG CATTTTCGAA
    GTGGTCTGGG CGGCCATCAA GCCAGGAACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG
    CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG
    TCCCTGCTCA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC
    GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT CCAGGATGAG
    ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG
    ACCGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AATCACAAGG TGGAGTCAGC
    AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG
    CTGAACGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG
    GACGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA
    GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCTGCCAG GCAGCAGAAC
    TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG
    CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGCCT GCGCTTCGCC
    ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG
    CCAGGCCGGG ATGGCGCGCG GGGGCCTGCG GGGGGCAAGG CCCGGCCCGA GGGCGGGGAG
    GCCGGCGAGG GTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACAGGGAC
    AAGGCCGCAG CCCCGGCGGG GGAGCCAGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG
    GAGCCGGGGG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA
    GGCCCCCGCG AGGCCAGGAG CGAGCGAGGC CGCGGGCTGG GCCCGGACGG CGGCCGGCGC
    CACCACCGGC GCGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG
    CACCGGCACG CGCCGGAGCC GAGCAGGGAG GGCGCGCCCG GGAGCAAGGG CGAGCGGCGA
    GCGCGGCACC GCGGCGGCTC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCC
    TCCCGGCGGC ACCGAGCGCG ACACAAGGCG CCGCCCGCAC ACGAGGAGGC GGAGAAGGAG
    GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGAAACCACC AGCCCAGGGA GTCGCACTGT
    GACCTGGAGG CCAGTGTGGT CATTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACTTGT
    CTAGAGAAGG TGGAAGAGCA GCCAGAGGAT GCAGACAATC AGCGGAATGT GACTCGCATG
    GGCAGTCAGC CCTCAGATCT GAGCACCGCT GTGCGTATCC CAGTGACGCT GACCGGGCCT
    CCCGGGGAGA CCACGGTTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA
    AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGTGGCC CCCGACCCAT TGTCCCGTAC
    AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTCCGCC GCTTCTGCCA CTACATTGTG
    ACCATGCGGT ACTTCGAGAT GGTCATTCTC GTGGTCATTG CCCTGAGTAG CATTGCCCTG
    GCTGCGGAGG ATCCTGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC
    TACATCTTCA CAGGTGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA TTTGGGACTG
    CTGCTGCACC CCGGTGCCTA CTTCCGGGAT CTGTGGAACA TTCTGGACTT CATTGTGGTC
    AGTGGGGCCC TGGTGGCGTT TGCCTTCTCG AGCTTCATGG GAGGCTCCAA AGGGAAAGAC
    ATCAGCACCA TCAAGTCCCT GAGGGTCCTG CGTGTGCTGA GGCCACTCAA GACCATCAAG
    CGGCTGCCCA AGCTCAAAGC TGTGTTTGAC TGTGTGGTGA ACTCCCTGAA GAACGTCCTC
    AACATCCTGA TCGTCTACAT GCTCTTCATG TTCATATTTG CTGTCATCGC GGTACAGCTC
    TTCAAGGGGA AGTTTTTCTA CTGCACCGAC GAGTCCAAGG AGCTGGAGCG GGACTGCAGG
    GGCCAGTATT TGGACTACGA GAAGGAGGAG GTGGAAGCTC AGCCACGGCA GTGGAAGAAA
    TACGACTTCC ACTATGACAA CGTGCTGTGG GCCCTGCTAA CGCTGTTCAC TGTGTCCACG
    GGCGAAGGGT GGCCCATGGT GCTGAAACAC TCGGTGGATG CCACCTACGA GGAGCAGGGC
    CCCAGCCCCG GGTTCCGCAT GGAGCTGTCC ATCTTCTATG TGGTCTACTT CGTGGTCTTC
    CCCTTCTTCT TTGTGAACAT CTTTGTGGCC TTGATCATCA TCACCTTCCA GGAGCAGGGG
    GACAAAGTCA TGTCTGAGTG CAGCTTGGAG AAGAACGAGA GGGCTTGTAT CGACTTCGCC
    ATTAGCGCCA AACCCCTGAC GCGGTACATG CCCCAGAACA AGCAGTCGTT CCAGTACAAG
    ACGTGGACGT TCGTGGTCTC ACCACCCTTT GAGTATTTCA TCATGGCCAT GATAGCCCTC
    AACACAGTGG TGCTGATGAT GAAGTTCTAC GATGCGCCCT ACGAGTACGA ACTGATGTTG
    AAGTGTCTGA ACATCGTGTT CACGTCCATG TTCTCCATGG AGTGTGTGCT CAAGGTCATC
    GCCTTTGGAG TGCTGAACTA TTTCAGAGAC GCCTGGAATG TCTTTGACTT TGTCACTGTG
    TTGGGAAGTA TTACTGATAT TTTAGTAACA GAGATTGCGA ACAACTTCAT CAACCTCAGC
    TTCCTCCGCC TCTTCCGTGC TGCCCGACTC ATCAAGCTGC TCCGCCAAGG CTACACCATC
    CGCATTCTGC TCTGGACCTT CGTCCAGTCC TTCAAGGCCC TGCCCTATGT GTGCCTGCTC
    ATCGCCATGC TGTTCTTCAT CTACGCCATC ATCGGCATGC AGGTGTTTGG AAACATTGCT
    CTGGATGACG ACACCAGCAT CAACCGTCAC AATAACTTTC GGACATTTCT TCAGGCCTTG
    ATGCTGCTCT TCAGGAGTGC CACCGGGGAG GCCTGGCATG AGATCATGCT GTCCTGCCTC
    AGCAGTCGGG CCTGTGATGA GCTCGCCAAT GCCACGGAGT GTGGGAGTGA CTTCGCCTAC
    TTCTACTTTG TCTCCTTCAT CTTCCTTTGC TCCTTTCTGA TGTTGAACCT CTTTGTGGCT
    GTGATCATGG ACAATTTTGA GTACCTTACG CGGGACTCTT CCATCCTAGG GCCTCACCAC
    CTGGACGAAT TCATCCGGGT CTGGGCCGAG TACGACCCGG CTGCGTGTGG GCGCATCAGT
    TACAATGACA TGTTTGAGAT GCTGAAACAC ATGTCCCCGC CCCTGGGGCT GGGGAAGAAA
    TGCCCTGCTC GTGTTGCGTA CAAGCGCCTG GTTCGTATGA ACATGCCCAT CTCCAATGAC
    GACATGACCG TCCACTTCAC ATCCACGCTG ATGGCCCTCA TCCGGACTGC ATTGGAGATC
    AAGCTGGCTC CAGCTGGCAC GAAGCAGCAT CAGTGTGACG CAGAGCTGAG GAAGGAGATC
    TCCTCCGTGT GGGCCAATCT GCCCCAGAAG ACCCTGGACT TACTGGTGCC ACCCCATAAA
    CCCGACGAGA TGACGGTGGG GAAGGTGTAC GCAGCTCTGA TGATATTCGA CTTCTACAAA
    CAGAACAAAA CCACCAGAGA CCAGATTCAC CAAGCTCCTG GAGGCCTGGC CCAGATGGGC
    CCCGTGTCCC TGTTCCACCC CCTGAAGGCC ACGCTGGAGC AGACTCAGCC GGCTGTGCTC
    CGAGGAGCCC GCGTTTTCCT TCGGCAGAAG AGCTCGGCCT CCCTCAGCAA CGGTGGGGCA
    GTACAAACCC AAGAGGGCGG CATCAAGGAG TCTGTCTCCT GGGGCACTCA GAGGACCCAG
    GAGGTCCCCT GCGAGGTGAG GACACCCCTG GAGCGTGGCC ACTCCACAGA GATCCCGGTG
    CAGACAGGAA AACCGGCTGT GGACGTCCAG ATGCAGAGCA TGGTCCTGAG AGGCCCTGAC
    GGGGAGCCCC AACCTGGGCT GGAGAGCCAG GGCCGAGCAG CTTCCATGCC CCGCCTGGCA
    GCAGAGACTC AGCCACCCCC AGATGCCAGC CCCATGAAGC GCTCCATCTC CACACTGGCA
    CCCCAGAGGC CCCATGTGGC TCATCTGTGC ACCGCGGCCC TGGACCGCGC TCCAGCCAGC
    CAGGCCGCCC CCCCCCACCA CCACCGCTGC CATCGCCGCA GGGACCGGAA GCAGAAGTCC
    CTGGAGAAGG GGCCCGGCCT GTCTGCAGAC ACAGATGGCG CGCCCTACAG CACCGCGGGG
    CCGGGCCCCC CGCCGCCCCC AGGGGATGGG GCAGCAGGCT GCCGGCGGGA GCGCAGGCAG
    GAGCGGGGCC GGTCCCAGGA GCGGAGGCAG CCCTCCTCCT CTTCCTCTGA GAAGCAGCGC
    TTCTACTCCT GTGACCGCTT TGGGGGCCGT GAGCCCCCGC AGCCCAAGCC CTCTCTCAGC
    AGCCATCCCA CGTCGCCCAC GGCCGGGCAG GAGCCAGGAC CCCCGAGACA GGGTAGCGGC
    TCCGTGAACG GGAGCCCCCT GCTGTCGACA TCTGGTGCTA GCACCCCTGG CCGCGGCGGG
    CGGAGGCAGC TCCCCCAGAC CCCGCTGACC CCCCGTCCCA GCATCACCTA TAAGACGGCC
    AACTCCTCAC CTGTCCACTT TGCCGGGGCT CAGACCAGCC TCCCTGCCTT TTCCCCCGGC
    CGGCTTAGCC GTGGCCTTTC GGAACACAAC GCCCTGCTCC AGAGAGACCC CCTCAGCCAG
    CCGCTGGCCC CCAGCTCTCG GATTGGCTCC GACCCTTACC TGGGGCAGCG TCTGGACAGT
    GAGGCTGCGG CCCGCACCCA GCTTGAGGAC ACGCTCACCT TCGAGGAGGC CGTGGCCACC
    AACTCGGGCC GCTCCTCCCG GACTTCCTAC GTGTCCTCCC TGACCTCGCA GTCCCACCCC
    CTCCGCCGGG TGCCCAACGG CTACCACTGC ACTCTGGGGC TCAGCTCGGG CGGGGGCGGC
    CGGGGGCGGC ACAGCTACCA CCACCCTGAC CAAGACCACT GGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220142
    Clone ID Related Accession (Same CDS sequence) XM_024998339.1
    Accession Version XM_024998339.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGCAGTGTT GAAGAGAGCA
    GCCACCAAGA AGAGCCGAAA CGACCTGATC CACGCGGAGG AGGGAGAGGA CCGCTTTGCA
    GACCTCTGTG CTGTGGGGTC CCCCTTCGCC CGAGCCAGCC TCAAGAGTGG GAAGACGGAG
    AGCTCGTCAT ACTTCCGCAG GAAGGAGAAG ATGTTCCGGT TCTTCATCCG GCGCCTGGTG
    AAGGCGCAGA GCTTCTACTG GGTGGTGCTG TGTGTGGTGG CCCTGAACAC GCTGTGCGTG
    GCCATGGTGC ACTACCAGCA GCCCCAGCGG CTCACCACGG CGCTGTACTT TGCAGAGTTT
    GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGG
    AGCTACTTCC GGTCCTCCTT CAACTGCTTC GATTTCGGGG TCATTGTGGG GAGCATTTTC
    GAAGTGGTCT GGGCGGCCAT CAAGCCAGGA ACCTCCTTCG GCATCAGTGT GCTGCGGGCG
    CTGCGGCTGC TGAGAATCTT CAAAGTCACC AAGTACTGGA GCTCCCTGCG GAACCTGGTG
    GTGTCCCTGC TCAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCCTGCT CTTCCTCTTC
    ATCGTGGTCT TCGCTCTGCT GGGCATGCAG CTCTTCGGGG GACAGTTCAA CTTCCAGGAT
    GAGACTCCGA CAACCAACTT TGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC
    CTGACCGGGG AGGACTGGAA TGCGGTGATG TACCATGGGA TCGAATCACA AGGTGGAGTC
    AGCAAGGGCA TGTTCTCGTC TGTGTACTTC ATTGTCCTGA CGCTGTTTGG GAACTACACC
    CTGCTGAACG TCTTCCTGGC CATCGCTGTG GACAACCTCG CCAATGCTCA GGAGCTGACC
    AAGGACGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA
    GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATTGCTGC CAGGCAGCAG
    AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTGCG GCTGCAGAAC
    CTGCGGGCCA GCTGTGAAGC GCTGTATAGC GAGATGGACC CCGAGGAGCG CCTGCGCTTC
    GCCACTTCCC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTTGTGGTG
    GAGCCAGGCC GGGATGGCGC GCGGGGGCCT GCGGGGGGCA AGGCCCGGCC CGAGGGCGGG
    GAGGCCGGCG AGGGTGCCGA CCCGCCGCGC AGGCACCACC GGCACCGCGA CAGGGACAGG
    GACAAGGCCG CAGCCCCGGC GGGGGAGCCA GACAGGGCGG ACGCCCCGAA GGCGGAGGGC
    GGGGAGCCGG GGGCCCGGGA GGAGCGGGCG CGACCGCGCC GCAGCCGCAG CAAGGAGGCC
    GCAGGCCCCC GCGAGGCCAG GAGCGAGCGA GGCCGCGGGC TGGGCCCGGA CGGCGGCCGG
    CGCCACCACC GGCGCGGCTC CCCGGAGGAG GCCGCGGAGC GCGAGCCCCG GCGCCACCGC
    GCGCACCGGC ACGCGCCGGA GCCGAGCAGG GAGGGCGCGC CCGGGAGCAA GGGCGAGCGG
    CGAGCGCGGC ACCGCGGCGG CTCCCGGGCC GGGCCCCGGG AGGCCGAGAG CGGCGAGGAG
    CCCTCCCGGC GGCACCGAGC GCGACACAAG GCGCCGCCCG CACACGAGGA GGCGGAGAAG
    GAGGCCGAGG CGGAGGACAG GGACAAGGAG CCCCGAAACC ACCAGCCCAG GGAGTCGCAC
    TGTGACCTGG AGGCCAGTGT GGTCATTGGT GTGGGCCCTG TGCATGCCCT GCCCAGCACT
    TGTCTAGAGA AGGTGGAAGA GCAGCCAGAG GATGCAGACA ATCAGCGGAA TGTGACTCGC
    ATGGGCAGTC AGCCCTCAGA TCTGAGCACC GCTGTGCGTA TCCCAGTGAC GCTGACCGGG
    CCTCCCGGGG AGACCACGGT TGTTCCCAGT GGTAACGTGG ACCTGGAAAG CCAAGCAGAA
    GGAAAGAAGG AGGTGGAAGC TGATGACGTG ATGAGGAGTG GCCCCCGACC CATTGTCCCG
    TACAGCTCCA TGTTCTGCTT GAGCCCCACC AACCTGCTCC GCCGCTTCTG CCACTACATT
    GTGACCATGC GGTACTTCGA GATGGTCATT CTCGTGGTCA TTGCCCTGAG TAGCATTGCC
    CTGGCTGCGG AGGATCCTGT GCAGACAGAC TCGCCCAGGA ACAACGTGCT GAAGTACATG
    GACTACATCT TCACAGGTGT CTTCACCTTT GAGATGGTGA TAAAGATGAT CGATTTGGGA
    CTGCTGCTGC ACCCCGGTGC CTACTTCCGG GATCTGTGGA ACATTCTGGA CTTCATTGTG
    GTCAGTGGGG CCCTGGTGGC GTTTGCCTTC TCAGGCTCCA AAGGGAAAGA CATCAGCACC
    ATCAAGTCCC TGAGGGTCCT GCGTGTGCTG AGGCCACTCA AGACCATCAA GCGGCTGCCC
    AAGCTCAAAG CTGTGTTTGA CTGTGTGGTG AACTCCCTGA AGAACGTCCT CAACATCCTG
    ATCGTCTACA TGCTCTTCAT GTTCATATTT GCTGTCATCG CGGTACAGCT CTTCAAGGGG
    AAGTTTTTCT ACTGCACCGA CGAGTCCAAG GAGCTGGAGC GGGACTGCAG GGGCCAGTAT
    TTGGACTACG AGAAGGAGGA GGTGGAAGCT CAGCCACGGC AGTGGAAGAA ATACGACTTC
    CACTATGACA ACGTGCTGTG GGCCCTGCTA ACGCTGTTCA CTGTGTCCAC GGGCGAAGGG
    TGGCCCATGG TGCTGAAACA CTCGGTGGAT GCCACCTACG AGGAGCAGGG CCCCAGCCCC
    GGGTTCCGCA TGGAGCTGTC CATCTTCTAT GTGGTCTACT TCGTGGTCTT CCCCTTCTTC
    TTTGTGAACA TCTTTGTGGC CTTGATCATC ATCACCTTCC AGGAGCAGGG GGACAAAGTC
    ATGTCTGAGT GCAGCTTGGA GAAGAACGAG AGGGCTTGTA TCGACTTCGC CATTAGCGCC
    AAACCCCTGA CGCGGTACAT GCCCCAGAAC AAGCAGTCGT TCCAGTACAA GACGTGGACG
    TTCGTGGTCT CACCACCCTT TGAGTATTTC ATCATGGCCA TGATAGCCCT CAACACAGTG
    GTGCTGATGA TGAAGTTCTA CGATGCGCCC TACGAGTACG AACTGATGTT GAAGTGTCTG
    AACATCGTGT TCACGTCCAT GTTCTCCATG GAGTGTGTGC TCAAGGTCAT CGCCTTTGGA
    GTGCTGAACT ATTTCAGAGA CGCCTGGAAT GTCTTTGACT TTGTCACTGT GTTGGGAAGT
    ATTACTGATA TTTTAGTAAC AGAGATTGCG GAAACGAACA ACTTCATCAA CCTCAGCTTC
    CTCCGCCTCT TCCGTGCTGC CCGACTCATC AAGCTGCTCC GCCAAGGCTA CACCATCCGC
    ATTCTGCTCT GGACCTTCGT CCAGTCCTTC AAGGCCCTGC CCTATGTGTG CCTGCTCATC
    GCCATGCTGT TCTTCATCTA CGCCATCATC GGCATGCAGG TGTTTGGAAA CATTGCTCTG
    GATGACGACA CCAGCATCAA CCGTCACAAT AACTTTCGGA CATTTCTTCA GGCCTTGATG
    CTGCTCTTCA GGAGTGCCAC CGGGGAGGCC TGGCATGAGA TCATGCTGTC CTGCCTCAGC
    AGTCGGGCCT GTGATGAGCT CGCCAATGCC ACGGAGTGTG GGAGTGACTT CGCCTACTTC
    TACTTTGTCT CCTTCATCTT CCTTTGCTCC TTTCTGATGT TGAACCTCTT TGTGGCTGTG
    ATCATGGACA ATTTTGAGTA CCTTACGCGG GACTCTTCCA TCCTAGGGCC TCACCACCTG
    GACGAATTCA TCCGGGTCTG GGCCGAGTAC GACCCGGCTG CGTGTGGGCG CATCAGTTAC
    AATGACATGT TTGAGATGCT GAAACACATG TCCCCGCCCC TGGGGCTGGG GAAGAAATGC
    CCTGCTCGTG TTGCGTACAA GCGCCTGGTT CGTATGAACA TGCCCATCTC CAATGACGAC
    ATGACCGTCC ACTTCACATC CACGCTGATG GCCCTCATCC GGACTGCATT GGAGATCAAG
    CTGGCTCCAG CTGGCACGAA GCAGCATCAG TGTGACGCAG AGCTGAGGAA GGAGATCTCC
    TCCGTGTGGG CCAATCTGCC CCAGAAGACC CTGGACTTAC TGGTGCCACC CCATAAACCC
    GACGAGATGA CGGTGGGGAA GGTGTACGCA GCTCTGATGA TATTCGACTT CTACAAACAG
    AACAAAACCA CCAGAGACCA GATTCACCAA GCTCCTGGAG GCCTGGCCCA GATGGGCCCC
    GTGTCCCTGT TCCACCCCCT GAAGGCCACG CTGGAGCAGA CTCAGCCGGC TGTGCTCCGA
    GGAGCCCGCG TTTTCCTTCG GCAGAAGAGC TCGGCCTCCC TCAGCAACGG TGGGGCAGTA
    CAAACCCAAG AGGGCGGCAT CAAGGAGTCT GTCTCCTGGG GCACTCAGAG GACCCAGGAG
    GTCCCCTGCG AGGTGAGGAC ACCCCTGGAG CGTGGCCACT CCACAGAGAT CCCGGTGCAG
    ACAGGAAAAC CGGCTGTGGA CGTCCAGATG CAGAGCATGG TCCTGAGAGG CCCTGACGGG
    GAGCCCCAAC CTGGGCTGGA GAGCCAGGGC CGAGCAGCTT CCATGCCCCG CCTGGCAGCA
    GAGACTCAGC CACCCCCAGA TGCCAGCCCC ATGAAGCGCT CCATCTCCAC ACTGGCACCC
    CAGAGGCCCC ATGTGGCTCA TCTGTGCACC GCGGCCCTGG ACCGCGCTCC AGCCAGCCAG
    GCCGCCCCCC CCCACCACCA CCGCTGCCAT CGCCGCAGGG ACCGGAAGCA GAAGTCCCTG
    GAGAAGGGGC CCGGCCTGTC TGCAGACACA GATGGCGCGC CCTACAGCAC CGCGGGGCCG
    GGCCCCCCGC CGCCCCCAGG GGATGGGGCA GCAGGCTGCC GGCGGGAGCG CAGGCAGGAG
    CGGGGCCGGT CCCAGGAGCG GAGGCAGCCC TCCTCCTCTT CCTCTGAGAA GCAGCGCTTC
    TACTCCTGTG ACCGCTTTGG GGGCCGTGAG CCCCCGCAGC CCAAGCCCTC TCTCAGCAGC
    CATCCCACGT CGCCCACGGC CGGGCAGGAG CCAGGACCCC CGAGACAGGG TAGCGGCTCC
    GTGAACGGGA GCCCCCTGCT GTCGACATCT GGTGCTAGCA CCCCTGGCCG CGGCGGGCGG
    AGGCAGCTCC CCCAGACCCC GCTGACCCCC CGTCCCAGCA TCACCTATAA GACGGCCAAC
    TCCTCACCTG TCCACTTTGC CGGGGCTCAG ACCAGCCTCC CTGCCTTTTC CCCCGGCCGG
    CTTAGCCGTG GCCTTTCGGA ACACAACGCC CTGCTCCAGA GAGACCCCCT CAGCCAGCCG
    CTGGCCCCCA GCTCTCGGAT TGGCTCCGAC CCTTACCTGG GGCAGCGTCT GGACAGTGAG
    GCTGCGGCCC GCACCCAGCT TGAGGACACG CTCACCTTCG AGGAGGCCGT GGCCACCAAC
    TCGGGCCGCT CCTCCCGGAC TTCCTACGTG TCCTCCCTGA CCTCGCAGTC CCACCCCCTC
    CGCCGGGTGC CCAACGGCTA CCACTGCACT CTGGGGCTCA GCTCGGGCGG GGGCGGCCGG
    GGGCGGCACA GCTACCACCA CCCTGACCAA GACCACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854107.1
    CDS140..7144
    Translation

    Target ORF information:

    RefSeq Version XM_024998339.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998339.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGCAGTGTT GAAGAGAGCA
    GCCACCAAGA AGAGCCGAAA CGACCTGATC CACGCGGAGG AGGGAGAGGA CCGCTTTGCA
    GACCTCTGTG CTGTGGGGTC CCCCTTCGCC CGAGCCAGCC TCAAGAGTGG GAAGACGGAG
    AGCTCGTCAT ACTTCCGCAG GAAGGAGAAG ATGTTCCGGT TCTTCATCCG GCGCCTGGTG
    AAGGCGCAGA GCTTCTACTG GGTGGTGCTG TGTGTGGTGG CCCTGAACAC GCTGTGCGTG
    GCCATGGTGC ACTACCAGCA GCCCCAGCGG CTCACCACGG CGCTGTACTT TGCAGAGTTT
    GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGG
    AGCTACTTCC GGTCCTCCTT CAACTGCTTC GATTTCGGGG TCATTGTGGG GAGCATTTTC
    GAAGTGGTCT GGGCGGCCAT CAAGCCAGGA ACCTCCTTCG GCATCAGTGT GCTGCGGGCG
    CTGCGGCTGC TGAGAATCTT CAAAGTCACC AAGTACTGGA GCTCCCTGCG GAACCTGGTG
    GTGTCCCTGC TCAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCCTGCT CTTCCTCTTC
    ATCGTGGTCT TCGCTCTGCT GGGCATGCAG CTCTTCGGGG GACAGTTCAA CTTCCAGGAT
    GAGACTCCGA CAACCAACTT TGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC
    CTGACCGGGG AGGACTGGAA TGCGGTGATG TACCATGGGA TCGAATCACA AGGTGGAGTC
    AGCAAGGGCA TGTTCTCGTC TGTGTACTTC ATTGTCCTGA CGCTGTTTGG GAACTACACC
    CTGCTGAACG TCTTCCTGGC CATCGCTGTG GACAACCTCG CCAATGCTCA GGAGCTGACC
    AAGGACGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA
    GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATTGCTGC CAGGCAGCAG
    AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTGCG GCTGCAGAAC
    CTGCGGGCCA GCTGTGAAGC GCTGTATAGC GAGATGGACC CCGAGGAGCG CCTGCGCTTC
    GCCACTTCCC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTTGTGGTG
    GAGCCAGGCC GGGATGGCGC GCGGGGGCCT GCGGGGGGCA AGGCCCGGCC CGAGGGCGGG
    GAGGCCGGCG AGGGTGCCGA CCCGCCGCGC AGGCACCACC GGCACCGCGA CAGGGACAGG
    GACAAGGCCG CAGCCCCGGC GGGGGAGCCA GACAGGGCGG ACGCCCCGAA GGCGGAGGGC
    GGGGAGCCGG GGGCCCGGGA GGAGCGGGCG CGACCGCGCC GCAGCCGCAG CAAGGAGGCC
    GCAGGCCCCC GCGAGGCCAG GAGCGAGCGA GGCCGCGGGC TGGGCCCGGA CGGCGGCCGG
    CGCCACCACC GGCGCGGCTC CCCGGAGGAG GCCGCGGAGC GCGAGCCCCG GCGCCACCGC
    GCGCACCGGC ACGCGCCGGA GCCGAGCAGG GAGGGCGCGC CCGGGAGCAA GGGCGAGCGG
    CGAGCGCGGC ACCGCGGCGG CTCCCGGGCC GGGCCCCGGG AGGCCGAGAG CGGCGAGGAG
    CCCTCCCGGC GGCACCGAGC GCGACACAAG GCGCCGCCCG CACACGAGGA GGCGGAGAAG
    GAGGCCGAGG CGGAGGACAG GGACAAGGAG CCCCGAAACC ACCAGCCCAG GGAGTCGCAC
    TGTGACCTGG AGGCCAGTGT GGTCATTGGT GTGGGCCCTG TGCATGCCCT GCCCAGCACT
    TGTCTAGAGA AGGTGGAAGA GCAGCCAGAG GATGCAGACA ATCAGCGGAA TGTGACTCGC
    ATGGGCAGTC AGCCCTCAGA TCTGAGCACC GCTGTGCGTA TCCCAGTGAC GCTGACCGGG
    CCTCCCGGGG AGACCACGGT TGTTCCCAGT GGTAACGTGG ACCTGGAAAG CCAAGCAGAA
    GGAAAGAAGG AGGTGGAAGC TGATGACGTG ATGAGGAGTG GCCCCCGACC CATTGTCCCG
    TACAGCTCCA TGTTCTGCTT GAGCCCCACC AACCTGCTCC GCCGCTTCTG CCACTACATT
    GTGACCATGC GGTACTTCGA GATGGTCATT CTCGTGGTCA TTGCCCTGAG TAGCATTGCC
    CTGGCTGCGG AGGATCCTGT GCAGACAGAC TCGCCCAGGA ACAACGTGCT GAAGTACATG
    GACTACATCT TCACAGGTGT CTTCACCTTT GAGATGGTGA TAAAGATGAT CGATTTGGGA
    CTGCTGCTGC ACCCCGGTGC CTACTTCCGG GATCTGTGGA ACATTCTGGA CTTCATTGTG
    GTCAGTGGGG CCCTGGTGGC GTTTGCCTTC TCAGGCTCCA AAGGGAAAGA CATCAGCACC
    ATCAAGTCCC TGAGGGTCCT GCGTGTGCTG AGGCCACTCA AGACCATCAA GCGGCTGCCC
    AAGCTCAAAG CTGTGTTTGA CTGTGTGGTG AACTCCCTGA AGAACGTCCT CAACATCCTG
    ATCGTCTACA TGCTCTTCAT GTTCATATTT GCTGTCATCG CGGTACAGCT CTTCAAGGGG
    AAGTTTTTCT ACTGCACCGA CGAGTCCAAG GAGCTGGAGC GGGACTGCAG GGGCCAGTAT
    TTGGACTACG AGAAGGAGGA GGTGGAAGCT CAGCCACGGC AGTGGAAGAA ATACGACTTC
    CACTATGACA ACGTGCTGTG GGCCCTGCTA ACGCTGTTCA CTGTGTCCAC GGGCGAAGGG
    TGGCCCATGG TGCTGAAACA CTCGGTGGAT GCCACCTACG AGGAGCAGGG CCCCAGCCCC
    GGGTTCCGCA TGGAGCTGTC CATCTTCTAT GTGGTCTACT TCGTGGTCTT CCCCTTCTTC
    TTTGTGAACA TCTTTGTGGC CTTGATCATC ATCACCTTCC AGGAGCAGGG GGACAAAGTC
    ATGTCTGAGT GCAGCTTGGA GAAGAACGAG AGGGCTTGTA TCGACTTCGC CATTAGCGCC
    AAACCCCTGA CGCGGTACAT GCCCCAGAAC AAGCAGTCGT TCCAGTACAA GACGTGGACG
    TTCGTGGTCT CACCACCCTT TGAGTATTTC ATCATGGCCA TGATAGCCCT CAACACAGTG
    GTGCTGATGA TGAAGTTCTA CGATGCGCCC TACGAGTACG AACTGATGTT GAAGTGTCTG
    AACATCGTGT TCACGTCCAT GTTCTCCATG GAGTGTGTGC TCAAGGTCAT CGCCTTTGGA
    GTGCTGAACT ATTTCAGAGA CGCCTGGAAT GTCTTTGACT TTGTCACTGT GTTGGGAAGT
    ATTACTGATA TTTTAGTAAC AGAGATTGCG GAAACGAACA ACTTCATCAA CCTCAGCTTC
    CTCCGCCTCT TCCGTGCTGC CCGACTCATC AAGCTGCTCC GCCAAGGCTA CACCATCCGC
    ATTCTGCTCT GGACCTTCGT CCAGTCCTTC AAGGCCCTGC CCTATGTGTG CCTGCTCATC
    GCCATGCTGT TCTTCATCTA CGCCATCATC GGCATGCAGG TGTTTGGAAA CATTGCTCTG
    GATGACGACA CCAGCATCAA CCGTCACAAT AACTTTCGGA CATTTCTTCA GGCCTTGATG
    CTGCTCTTCA GGAGTGCCAC CGGGGAGGCC TGGCATGAGA TCATGCTGTC CTGCCTCAGC
    AGTCGGGCCT GTGATGAGCT CGCCAATGCC ACGGAGTGTG GGAGTGACTT CGCCTACTTC
    TACTTTGTCT CCTTCATCTT CCTTTGCTCC TTTCTGATGT TGAACCTCTT TGTGGCTGTG
    ATCATGGACA ATTTTGAGTA CCTTACGCGG GACTCTTCCA TCCTAGGGCC TCACCACCTG
    GACGAATTCA TCCGGGTCTG GGCCGAGTAC GACCCGGCTG CGTGTGGGCG CATCAGTTAC
    AATGACATGT TTGAGATGCT GAAACACATG TCCCCGCCCC TGGGGCTGGG GAAGAAATGC
    CCTGCTCGTG TTGCGTACAA GCGCCTGGTT CGTATGAACA TGCCCATCTC CAATGACGAC
    ATGACCGTCC ACTTCACATC CACGCTGATG GCCCTCATCC GGACTGCATT GGAGATCAAG
    CTGGCTCCAG CTGGCACGAA GCAGCATCAG TGTGACGCAG AGCTGAGGAA GGAGATCTCC
    TCCGTGTGGG CCAATCTGCC CCAGAAGACC CTGGACTTAC TGGTGCCACC CCATAAACCC
    GACGAGATGA CGGTGGGGAA GGTGTACGCA GCTCTGATGA TATTCGACTT CTACAAACAG
    AACAAAACCA CCAGAGACCA GATTCACCAA GCTCCTGGAG GCCTGGCCCA GATGGGCCCC
    GTGTCCCTGT TCCACCCCCT GAAGGCCACG CTGGAGCAGA CTCAGCCGGC TGTGCTCCGA
    GGAGCCCGCG TTTTCCTTCG GCAGAAGAGC TCGGCCTCCC TCAGCAACGG TGGGGCAGTA
    CAAACCCAAG AGGGCGGCAT CAAGGAGTCT GTCTCCTGGG GCACTCAGAG GACCCAGGAG
    GTCCCCTGCG AGGTGAGGAC ACCCCTGGAG CGTGGCCACT CCACAGAGAT CCCGGTGCAG
    ACAGGAAAAC CGGCTGTGGA CGTCCAGATG CAGAGCATGG TCCTGAGAGG CCCTGACGGG
    GAGCCCCAAC CTGGGCTGGA GAGCCAGGGC CGAGCAGCTT CCATGCCCCG CCTGGCAGCA
    GAGACTCAGC CACCCCCAGA TGCCAGCCCC ATGAAGCGCT CCATCTCCAC ACTGGCACCC
    CAGAGGCCCC ATGTGGCTCA TCTGTGCACC GCGGCCCTGG ACCGCGCTCC AGCCAGCCAG
    GCCGCCCCCC CCCACCACCA CCGCTGCCAT CGCCGCAGGG ACCGGAAGCA GAAGTCCCTG
    GAGAAGGGGC CCGGCCTGTC TGCAGACACA GATGGCGCGC CCTACAGCAC CGCGGGGCCG
    GGCCCCCCGC CGCCCCCAGG GGATGGGGCA GCAGGCTGCC GGCGGGAGCG CAGGCAGGAG
    CGGGGCCGGT CCCAGGAGCG GAGGCAGCCC TCCTCCTCTT CCTCTGAGAA GCAGCGCTTC
    TACTCCTGTG ACCGCTTTGG GGGCCGTGAG CCCCCGCAGC CCAAGCCCTC TCTCAGCAGC
    CATCCCACGT CGCCCACGGC CGGGCAGGAG CCAGGACCCC CGAGACAGGG TAGCGGCTCC
    GTGAACGGGA GCCCCCTGCT GTCGACATCT GGTGCTAGCA CCCCTGGCCG CGGCGGGCGG
    AGGCAGCTCC CCCAGACCCC GCTGACCCCC CGTCCCAGCA TCACCTATAA GACGGCCAAC
    TCCTCACCTG TCCACTTTGC CGGGGCTCAG ACCAGCCTCC CTGCCTTTTC CCCCGGCCGG
    CTTAGCCGTG GCCTTTCGGA ACACAACGCC CTGCTCCAGA GAGACCCCCT CAGCCAGCCG
    CTGGCCCCCA GCTCTCGGAT TGGCTCCGAC CCTTACCTGG GGCAGCGTCT GGACAGTGAG
    GCTGCGGCCC GCACCCAGCT TGAGGACACG CTCACCTTCG AGGAGGCCGT GGCCACCAAC
    TCGGGCCGCT CCTCCCGGAC TTCCTACGTG TCCTCCCTGA CCTCGCAGTC CCACCCCCTC
    CGCCGGGTGC CCAACGGCTA CCACTGCACT CTGGGGCTCA GCTCGGGCGG GGGCGGCCGG
    GGGCGGCACA GCTACCACCA CCCTGACCAA GACCACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220143
    Clone ID Related Accession (Same CDS sequence) XM_024998340.1
    Accession Version XM_024998340.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC
    ACCAAGAAGA GCCGAAACGA CCTGATCCAC GCGGAGGAGG GAGAGGACCG CTTTGCAGAC
    CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGCCTCA AGAGTGGGAA GACGGAGAGC
    TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG
    GCGCAGAGCT TCTACTGGGT GGTGCTGTGT GTGGTGGCCC TGAACACGCT GTGCGTGGCC
    ATGGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCGC TGTACTTTGC AGAGTTTGTT
    TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGGAGC
    TACTTCCGGT CCTCCTTCAA CTGCTTCGAT TTCGGGGTCA TTGTGGGGAG CATTTTCGAA
    GTGGTCTGGG CGGCCATCAA GCCAGGAACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG
    CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG
    TCCCTGCTCA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC
    GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT CCAGGATGAG
    ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG
    ACCGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AATCACAAGG TGGAGTCAGC
    AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG
    CTGAACGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG
    GACGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA
    GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCTGCCAG GCAGCAGAAC
    TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG
    CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGCCT GCGCTTCGCC
    ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG
    CCAGGCCGGG ATGGCGCGCG GGGGCCTGCG GGGGGCAAGG CCCGGCCCGA GGGCGGGGAG
    GCCGGCGAGG GTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACAGGGAC
    AAGGCCGCAG CCCCGGCGGG GGAGCCAGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG
    GAGCCGGGGG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA
    GGCCCCCGCG AGGCCAGGAG CGAGCGAGGC CGCGGGCTGG GCCCGGACGG CGGCCGGCGC
    CACCACCGGC GCGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG
    CACCGGCACG CGCCGGAGCC GAGCAGGGAG GGCGCGCCCG GGAGCAAGGG CGAGCGGCGA
    GCGCGGCACC GCGGCGGCTC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCC
    TCCCGGCGGC ACCGAGCGCG ACACAAGGCG CCGCCCGCAC ACGAGGAGGC GGAGAAGGAG
    GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGAAACCACC AGCCCAGGGA GTCGCACTGT
    GACCTGGAGG CCAGTGTGGT CATTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACTTGT
    CTAGAGAAGG TGGAAGAGCA GCCAGAGGAT GCAGACAATC AGCGGAATGT GACTCGCATG
    GGCAGTCAGC CCTCAGATCT GAGCACCGCT GTGCGTATCC CAGTGACGCT GACCGGGCCT
    CCCGGGGAGA CCACGGTTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA
    AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGTGGCC CCCGACCCAT TGTCCCGTAC
    AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTCCGCC GCTTCTGCCA CTACATTGTG
    ACCATGCGGT ACTTCGAGAT GGTCATTCTC GTGGTCATTG CCCTGAGTAG CATTGCCCTG
    GCTGCGGAGG ATCCTGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC
    TACATCTTCA CAGGTGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA TTTGGGACTG
    CTGCTGCACC CCGGTGCCTA CTTCCGGGAT CTGTGGAACA TTCTGGACTT CATTGTGGTC
    AGTGGGGCCC TGGTGGCGTT TGCCTTCTCA GGCTCCAAAG GGAAAGACAT CAGCACCATC
    AAGTCCCTGA GGGTCCTGCG TGTGCTGAGG CCACTCAAGA CCATCAAGCG GCTGCCCAAG
    CTCAAAGCTG TGTTTGACTG TGTGGTGAAC TCCCTGAAGA ACGTCCTCAA CATCCTGATC
    GTCTACATGC TCTTCATGTT CATATTTGCT GTCATCGCGG TACAGCTCTT CAAGGGGAAG
    TTTTTCTACT GCACCGACGA GTCCAAGGAG CTGGAGCGGG ACTGCAGGGG CCAGTATTTG
    GACTACGAGA AGGAGGAGGT GGAAGCTCAG CCACGGCAGT GGAAGAAATA CGACTTCCAC
    TATGACAACG TGCTGTGGGC CCTGCTAACG CTGTTCACTG TGTCCACGGG CGAAGGGTGG
    CCCATGGTGC TGAAACACTC GGTGGATGCC ACCTACGAGG AGCAGGGCCC CAGCCCCGGG
    TTCCGCATGG AGCTGTCCAT CTTCTATGTG GTCTACTTCG TGGTCTTCCC CTTCTTCTTT
    GTGAACATCT TTGTGGCCTT GATCATCATC ACCTTCCAGG AGCAGGGGGA CAAAGTCATG
    TCTGAGTGCA GCTTGGAGAA GAACGAGAGG GCTTGTATCG ACTTCGCCAT TAGCGCCAAA
    CCCCTGACGC GGTACATGCC CCAGAACAAG CAGTCGTTCC AGTACAAGAC GTGGACGTTC
    GTGGTCTCAC CACCCTTTGA GTATTTCATC ATGGCCATGA TAGCCCTCAA CACAGTGGTG
    CTGATGATGA AGTTCTACGA TGCGCCCTAC GAGTACGAAC TGATGTTGAA GTGTCTGAAC
    ATCGTGTTCA CGTCCATGTT CTCCATGGAG TGTGTGCTCA AGGTCATCGC CTTTGGAGTG
    CTGAACTATT TCAGAGACGC CTGGAATGTC TTTGACTTTG TCACTGTGTT GGGAAGTATT
    ACTGATATTT TAGTAACAGA GATTGCGGAA ACGAACAACT TCATCAACCT CAGCTTCCTC
    CGCCTCTTCC GTGCTGCCCG ACTCATCAAG CTGCTCCGCC AAGGCTACAC CATCCGCATT
    CTGCTCTGGA CCTTCGTCCA GTCCTTCAAG GCCCTGCCCT ATGTGTGCCT GCTCATCGCC
    ATGCTGTTCT TCATCTACGC CATCATCGGC ATGCAGGTGT TTGGAAACAT TGCTCTGGAT
    GACGACACCA GCATCAACCG TCACAATAAC TTTCGGACAT TTCTTCAGGC CTTGATGCTG
    CTCTTCAGGA GTGCCACCGG GGAGGCCTGG CATGAGATCA TGCTGTCCTG CCTCAGCAGT
    CGGGCCTGTG ATGAGCTCGC CAATGCCACG GAGTGTGGGA GTGACTTCGC CTACTTCTAC
    TTTGTCTCCT TCATCTTCCT TTGCTCCTTT CTGATGTTGA ACCTCTTTGT GGCTGTGATC
    ATGGACAATT TTGAGTACCT TACGCGGGAC TCTTCCATCC TAGGGCCTCA CCACCTGGAC
    GAATTCATCC GGGTCTGGGC CGAGTACGAC CCGGCTGCGT GTGGGCGCAT CAGTTACAAT
    GACATGTTTG AGATGCTGAA ACACATGTCC CCGCCCCTGG GGCTGGGGAA GAAATGCCCT
    GCTCGTGTTG CGTACAAGCG CCTGGTTCGT ATGAACATGC CCATCTCCAA TGACGACATG
    ACCGTCCACT TCACATCCAC GCTGATGGCC CTCATCCGGA CTGCATTGGA GATCAAGCTG
    GCTCCAGCTG GCACGAAGCA GCATCAGTGT GACGCAGAGC TGAGGAAGGA GATCTCCTCC
    GTGTGGGCCA ATCTGCCCCA GAAGACCCTG GACTTACTGG TGCCACCCCA TAAACCCGAC
    GAGATGACGG TGGGGAAGGT GTACGCAGCT CTGATGATAT TCGACTTCTA CAAACAGAAC
    AAAACCACCA GAGACCAGAT TCACCAAGCT CCTGGAGGCC TGGCCCAGAT GGGCCCCGTG
    TCCCTGTTCC ACCCCCTGAA GGCCACGCTG GAGCAGACTC AGCCGGCTGT GCTCCGAGGA
    GCCCGCGTTT TCCTTCGGCA GAAGAGCTCG GCCTCCCTCA GCAACGGTGG GGCAGTACAA
    ACCCAAGAGG GCGGCATCAA GGAGTCTGTC TCCTGGGGCA CTCAGAGGAC CCAGGAGGTC
    CCCTGCGAGG TGAGGACACC CCTGGAGCGT GGCCACTCCA CAGAGATCCC GGTGCAGACA
    GGAAAACCGG CTGTGGACGT CCAGATGCAG AGCATGGTCC TGAGAGGCCC TGACGGGGAG
    CCCCAACCTG GGCTGGAGAG CCAGGGCCGA GCAGCTTCCA TGCCCCGCCT GGCAGCAGAG
    ACTCAGCCAC CCCCAGATGC CAGCCCCATG AAGCGCTCCA TCTCCACACT GGCACCCCAG
    AGGCCCCATG TGGCTCATCT GTGCACCGCG GCCCTGGACC GCGCTCCAGC CAGCCAGGCC
    GCCCCCCCCC ACCACCACCG CTGCCATCGC CGCAGGGACC GGAAGCAGAA GTCCCTGGAG
    AAGGGGCCCG GCCTGTCTGC AGACACAGAT GGCGCGCCCT ACAGCACCGC GGGGCCGGGC
    CCCCCGCCGC CCCCAGGGGA TGGGGCAGCA GGCTGCCGGC GGGAGCGCAG GCAGGAGCGG
    GGCCGGTCCC AGGAGCGGAG GCAGCCCTCC TCCTCTTCCT CTGAGAAGCA GCGCTTCTAC
    TCCTGTGACC GCTTTGGGGG CCGTGAGCCC CCGCAGCCCA AGCCCTCTCT CAGCAGCCAT
    CCCACGTCGC CCACGGCCGG GCAGGAGCCA GGACCCCCGA GACAGGGTAG CGGCTCCGTG
    AACGGGAGCC CCCTGCTGTC GACATCTGGT GCTAGCACCC CTGGCCGCGG CGGGCGGAGG
    CAGCTCCCCC AGACCCCGCT GACCCCCCGT CCCAGCATCA CCTATAAGAC GGCCAACTCC
    TCACCTGTCC ACTTTGCCGG GGCTCAGACC AGCCTCCCTG CCTTTTCCCC CGGCCGGCTT
    AGCCGTGGCC TTTCGGAACA CAACGCCCTG CTCCAGAGAG ACCCCCTCAG CCAGCCGCTG
    GCCCCCAGCT CTCGGATTGG CTCCGACCCT TACCTGGGGC AGCGTCTGGA CAGTGAGGCT
    GCGGCCCGCA CCCAGCTTGA GGACACGCTC ACCTTCGAGG AGGCCGTGGC CACCAACTCG
    GGCCGCTCCT CCCGGACTTC CTACGTGTCC TCCCTGACCT CGCAGTCCCA CCCCCTCCGC
    CGGGTGCCCA ACGGCTACCA CTGCACTCTG GGGCTCAGCT CGGGCGGGGG CGGCCGGGGG
    CGGCACAGCT ACCACCACCC TGACCAAGAC CACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854108.1
    CDS1..7002
    Translation

    Target ORF information:

    RefSeq Version XM_024998340.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998340.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC
    ACCAAGAAGA GCCGAAACGA CCTGATCCAC GCGGAGGAGG GAGAGGACCG CTTTGCAGAC
    CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGCCTCA AGAGTGGGAA GACGGAGAGC
    TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG
    GCGCAGAGCT TCTACTGGGT GGTGCTGTGT GTGGTGGCCC TGAACACGCT GTGCGTGGCC
    ATGGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCGC TGTACTTTGC AGAGTTTGTT
    TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGGAGC
    TACTTCCGGT CCTCCTTCAA CTGCTTCGAT TTCGGGGTCA TTGTGGGGAG CATTTTCGAA
    GTGGTCTGGG CGGCCATCAA GCCAGGAACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG
    CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG
    TCCCTGCTCA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC
    GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT CCAGGATGAG
    ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG
    ACCGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AATCACAAGG TGGAGTCAGC
    AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG
    CTGAACGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG
    GACGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA
    GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCTGCCAG GCAGCAGAAC
    TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG
    CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGCCT GCGCTTCGCC
    ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG
    CCAGGCCGGG ATGGCGCGCG GGGGCCTGCG GGGGGCAAGG CCCGGCCCGA GGGCGGGGAG
    GCCGGCGAGG GTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACAGGGAC
    AAGGCCGCAG CCCCGGCGGG GGAGCCAGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG
    GAGCCGGGGG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA
    GGCCCCCGCG AGGCCAGGAG CGAGCGAGGC CGCGGGCTGG GCCCGGACGG CGGCCGGCGC
    CACCACCGGC GCGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG
    CACCGGCACG CGCCGGAGCC GAGCAGGGAG GGCGCGCCCG GGAGCAAGGG CGAGCGGCGA
    GCGCGGCACC GCGGCGGCTC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCC
    TCCCGGCGGC ACCGAGCGCG ACACAAGGCG CCGCCCGCAC ACGAGGAGGC GGAGAAGGAG
    GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGAAACCACC AGCCCAGGGA GTCGCACTGT
    GACCTGGAGG CCAGTGTGGT CATTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACTTGT
    CTAGAGAAGG TGGAAGAGCA GCCAGAGGAT GCAGACAATC AGCGGAATGT GACTCGCATG
    GGCAGTCAGC CCTCAGATCT GAGCACCGCT GTGCGTATCC CAGTGACGCT GACCGGGCCT
    CCCGGGGAGA CCACGGTTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA
    AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGTGGCC CCCGACCCAT TGTCCCGTAC
    AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTCCGCC GCTTCTGCCA CTACATTGTG
    ACCATGCGGT ACTTCGAGAT GGTCATTCTC GTGGTCATTG CCCTGAGTAG CATTGCCCTG
    GCTGCGGAGG ATCCTGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC
    TACATCTTCA CAGGTGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA TTTGGGACTG
    CTGCTGCACC CCGGTGCCTA CTTCCGGGAT CTGTGGAACA TTCTGGACTT CATTGTGGTC
    AGTGGGGCCC TGGTGGCGTT TGCCTTCTCA GGCTCCAAAG GGAAAGACAT CAGCACCATC
    AAGTCCCTGA GGGTCCTGCG TGTGCTGAGG CCACTCAAGA CCATCAAGCG GCTGCCCAAG
    CTCAAAGCTG TGTTTGACTG TGTGGTGAAC TCCCTGAAGA ACGTCCTCAA CATCCTGATC
    GTCTACATGC TCTTCATGTT CATATTTGCT GTCATCGCGG TACAGCTCTT CAAGGGGAAG
    TTTTTCTACT GCACCGACGA GTCCAAGGAG CTGGAGCGGG ACTGCAGGGG CCAGTATTTG
    GACTACGAGA AGGAGGAGGT GGAAGCTCAG CCACGGCAGT GGAAGAAATA CGACTTCCAC
    TATGACAACG TGCTGTGGGC CCTGCTAACG CTGTTCACTG TGTCCACGGG CGAAGGGTGG
    CCCATGGTGC TGAAACACTC GGTGGATGCC ACCTACGAGG AGCAGGGCCC CAGCCCCGGG
    TTCCGCATGG AGCTGTCCAT CTTCTATGTG GTCTACTTCG TGGTCTTCCC CTTCTTCTTT
    GTGAACATCT TTGTGGCCTT GATCATCATC ACCTTCCAGG AGCAGGGGGA CAAAGTCATG
    TCTGAGTGCA GCTTGGAGAA GAACGAGAGG GCTTGTATCG ACTTCGCCAT TAGCGCCAAA
    CCCCTGACGC GGTACATGCC CCAGAACAAG CAGTCGTTCC AGTACAAGAC GTGGACGTTC
    GTGGTCTCAC CACCCTTTGA GTATTTCATC ATGGCCATGA TAGCCCTCAA CACAGTGGTG
    CTGATGATGA AGTTCTACGA TGCGCCCTAC GAGTACGAAC TGATGTTGAA GTGTCTGAAC
    ATCGTGTTCA CGTCCATGTT CTCCATGGAG TGTGTGCTCA AGGTCATCGC CTTTGGAGTG
    CTGAACTATT TCAGAGACGC CTGGAATGTC TTTGACTTTG TCACTGTGTT GGGAAGTATT
    ACTGATATTT TAGTAACAGA GATTGCGGAA ACGAACAACT TCATCAACCT CAGCTTCCTC
    CGCCTCTTCC GTGCTGCCCG ACTCATCAAG CTGCTCCGCC AAGGCTACAC CATCCGCATT
    CTGCTCTGGA CCTTCGTCCA GTCCTTCAAG GCCCTGCCCT ATGTGTGCCT GCTCATCGCC
    ATGCTGTTCT TCATCTACGC CATCATCGGC ATGCAGGTGT TTGGAAACAT TGCTCTGGAT
    GACGACACCA GCATCAACCG TCACAATAAC TTTCGGACAT TTCTTCAGGC CTTGATGCTG
    CTCTTCAGGA GTGCCACCGG GGAGGCCTGG CATGAGATCA TGCTGTCCTG CCTCAGCAGT
    CGGGCCTGTG ATGAGCTCGC CAATGCCACG GAGTGTGGGA GTGACTTCGC CTACTTCTAC
    TTTGTCTCCT TCATCTTCCT TTGCTCCTTT CTGATGTTGA ACCTCTTTGT GGCTGTGATC
    ATGGACAATT TTGAGTACCT TACGCGGGAC TCTTCCATCC TAGGGCCTCA CCACCTGGAC
    GAATTCATCC GGGTCTGGGC CGAGTACGAC CCGGCTGCGT GTGGGCGCAT CAGTTACAAT
    GACATGTTTG AGATGCTGAA ACACATGTCC CCGCCCCTGG GGCTGGGGAA GAAATGCCCT
    GCTCGTGTTG CGTACAAGCG CCTGGTTCGT ATGAACATGC CCATCTCCAA TGACGACATG
    ACCGTCCACT TCACATCCAC GCTGATGGCC CTCATCCGGA CTGCATTGGA GATCAAGCTG
    GCTCCAGCTG GCACGAAGCA GCATCAGTGT GACGCAGAGC TGAGGAAGGA GATCTCCTCC
    GTGTGGGCCA ATCTGCCCCA GAAGACCCTG GACTTACTGG TGCCACCCCA TAAACCCGAC
    GAGATGACGG TGGGGAAGGT GTACGCAGCT CTGATGATAT TCGACTTCTA CAAACAGAAC
    AAAACCACCA GAGACCAGAT TCACCAAGCT CCTGGAGGCC TGGCCCAGAT GGGCCCCGTG
    TCCCTGTTCC ACCCCCTGAA GGCCACGCTG GAGCAGACTC AGCCGGCTGT GCTCCGAGGA
    GCCCGCGTTT TCCTTCGGCA GAAGAGCTCG GCCTCCCTCA GCAACGGTGG GGCAGTACAA
    ACCCAAGAGG GCGGCATCAA GGAGTCTGTC TCCTGGGGCA CTCAGAGGAC CCAGGAGGTC
    CCCTGCGAGG TGAGGACACC CCTGGAGCGT GGCCACTCCA CAGAGATCCC GGTGCAGACA
    GGAAAACCGG CTGTGGACGT CCAGATGCAG AGCATGGTCC TGAGAGGCCC TGACGGGGAG
    CCCCAACCTG GGCTGGAGAG CCAGGGCCGA GCAGCTTCCA TGCCCCGCCT GGCAGCAGAG
    ACTCAGCCAC CCCCAGATGC CAGCCCCATG AAGCGCTCCA TCTCCACACT GGCACCCCAG
    AGGCCCCATG TGGCTCATCT GTGCACCGCG GCCCTGGACC GCGCTCCAGC CAGCCAGGCC
    GCCCCCCCCC ACCACCACCG CTGCCATCGC CGCAGGGACC GGAAGCAGAA GTCCCTGGAG
    AAGGGGCCCG GCCTGTCTGC AGACACAGAT GGCGCGCCCT ACAGCACCGC GGGGCCGGGC
    CCCCCGCCGC CCCCAGGGGA TGGGGCAGCA GGCTGCCGGC GGGAGCGCAG GCAGGAGCGG
    GGCCGGTCCC AGGAGCGGAG GCAGCCCTCC TCCTCTTCCT CTGAGAAGCA GCGCTTCTAC
    TCCTGTGACC GCTTTGGGGG CCGTGAGCCC CCGCAGCCCA AGCCCTCTCT CAGCAGCCAT
    CCCACGTCGC CCACGGCCGG GCAGGAGCCA GGACCCCCGA GACAGGGTAG CGGCTCCGTG
    AACGGGAGCC CCCTGCTGTC GACATCTGGT GCTAGCACCC CTGGCCGCGG CGGGCGGAGG
    CAGCTCCCCC AGACCCCGCT GACCCCCCGT CCCAGCATCA CCTATAAGAC GGCCAACTCC
    TCACCTGTCC ACTTTGCCGG GGCTCAGACC AGCCTCCCTG CCTTTTCCCC CGGCCGGCTT
    AGCCGTGGCC TTTCGGAACA CAACGCCCTG CTCCAGAGAG ACCCCCTCAG CCAGCCGCTG
    GCCCCCAGCT CTCGGATTGG CTCCGACCCT TACCTGGGGC AGCGTCTGGA CAGTGAGGCT
    GCGGCCCGCA CCCAGCTTGA GGACACGCTC ACCTTCGAGG AGGCCGTGGC CACCAACTCG
    GGCCGCTCCT CCCGGACTTC CTACGTGTCC TCCCTGACCT CGCAGTCCCA CCCCCTCCGC
    CGGGTGCCCA ACGGCTACCA CTGCACTCTG GGGCTCAGCT CGGGCGGGGG CGGCCGGGGG
    CGGCACAGCT ACCACCACCC TGACCAAGAC CACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220144
    Clone ID Related Accession (Same CDS sequence) XM_024998341.1
    Accession Version XM_024998341.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGCAGTGTT GAAGAGAGCA
    GCCACCAAGA AGAGCCGAAA CGACCTGATC CACGCGGAGG AGGGAGAGGA CCGCTTTGCA
    GACCTCTGTG CTGTGGGGTC CCCCTTCGCC CGAGCCAGCC TCAAGAGTGG GAAGACGGAG
    AGCTCGTCAT ACTTCCGCAG GAAGGAGAAG ATGTTCCGGT TCTTCATCCG GCGCCTGGTG
    AAGGCGCAGA GCTTCTACTG GGTGGTGCTG TGTGTGGTGG CCCTGAACAC GCTGTGCGTG
    GCCATGGTGC ACTACCAGCA GCCCCAGCGG CTCACCACGG CGCTGTACTT TGCAGAGTTT
    GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGG
    AGCTACTTCC GGTCCTCCTT CAACTGCTTC GATTTCGGGG TCATTGTGGG GAGCATTTTC
    GAAGTGGTCT GGGCGGCCAT CAAGCCAGGA ACCTCCTTCG GCATCAGTGT GCTGCGGGCG
    CTGCGGCTGC TGAGAATCTT CAAAGTCACC AAGTACTGGA GCTCCCTGCG GAACCTGGTG
    GTGTCCCTGC TCAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCCTGCT CTTCCTCTTC
    ATCGTGGTCT TCGCTCTGCT GGGCATGCAG CTCTTCGGGG GACAGTTCAA CTTCCAGGAT
    GAGACTCCGA CAACCAACTT TGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC
    CTGACCGGGG AGGACTGGAA TGCGGTGATG TACCATGGGA TCGAATCACA AGGTGGAGTC
    AGCAAGGGCA TGTTCTCGTC TGTGTACTTC ATTGTCCTGA CGCTGTTTGG GAACTACACC
    CTGCTGAACG TCTTCCTGGC CATCGCTGTG GACAACCTCG CCAATGCTCA GGAGCTGACC
    AAGGACGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA
    GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATTGCTGC CAGGCAGCAG
    AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTGCG GCTGCAGAAC
    CTGCGGGCCA GCTGTGAAGC GCTGTATAGC GAGATGGACC CCGAGGAGCG CCTGCGCTTC
    GCCACTTCCC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTTGTGGTG
    GAGCCAGGCC GGGATGGCGC GCGGGGGCCT GCGGGGGGCA AGGCCCGGCC CGAGGGCGGG
    GAGGCCGGCG AGGGTGCCGA CCCGCCGCGC AGGCACCACC GGCACCGCGA CAGGGACAGG
    GACAAGGCCG CAGCCCCGGC GGGGGAGCCA GACAGGGCGG ACGCCCCGAA GGCGGAGGGC
    GGGGAGCCGG GGGCCCGGGA GGAGCGGGCG CGACCGCGCC GCAGCCGCAG CAAGGAGGCC
    GCAGGCCCCC GCGAGGCCAG GAGCGAGCGA GGCCGCGGGC TGGGCCCGGA CGGCGGCCGG
    CGCCACCACC GGCGCGGCTC CCCGGAGGAG GCCGCGGAGC GCGAGCCCCG GCGCCACCGC
    GCGCACCGGC ACGCGCCGGA GCCGAGCAGG GAGGGCGCGC CCGGGAGCAA GGGCGAGCGG
    CGAGCGCGGC ACCGCGGCGG CTCCCGGGCC GGGCCCCGGG AGGCCGAGAG CGGCGAGGAG
    CCCTCCCGGC GGCACCGAGC GCGACACAAG GCGCCGCCCG CACACGAGGA GGCGGAGAAG
    GAGGCCGAGG CGGAGGACAG GGACAAGGAG CCCCGAAACC ACCAGCCCAG GGAGTCGCAC
    TGTGACCTGG AGGCCAGTGT GGTCATTGGT GTGGGCCCTG TGCATGCCCT GCCCAGCACT
    TGTCTAGAGA AGGTGGAAGA GCAGCCAGAG GATGCAGACA ATCAGCGGAA TGTGACTCGC
    ATGGGCAGTC AGCCCTCAGA TCTGAGCACC GCTGTGCGTA TCCCAGTGAC GCTGACCGGG
    CCTCCCGGGG AGACCACGGT TGTTCCCAGT GGTAACGTGG ACCTGGAAAG CCAAGCAGAA
    GGAAAGAAGG AGGTGGAAGC TGATGACGTG ATGAGGAGTG GCCCCCGACC CATTGTCCCG
    TACAGCTCCA TGTTCTGCTT GAGCCCCACC AACCTGCTCC GCCGCTTCTG CCACTACATT
    GTGACCATGC GGTACTTCGA GATGGTCATT CTCGTGGTCA TTGCCCTGAG TAGCATTGCC
    CTGGCTGCGG AGGATCCTGT GCAGACAGAC TCGCCCAGGA ACAACGTGCT GAAGTACATG
    GACTACATCT TCACAGGTGT CTTCACCTTT GAGATGGTGA TAAAGATGAT CGATTTGGGA
    CTGCTGCTGC ACCCCGGTGC CTACTTCCGG GATCTGTGGA ACATTCTGGA CTTCATTGTG
    GTCAGTGGGG CCCTGGTGGC GTTTGCCTTC TCAGGCTCCA AAGGGAAAGA CATCAGCACC
    ATCAAGTCCC TGAGGGTCCT GCGTGTGCTG AGGCCACTCA AGACCATCAA GCGGCTGCCC
    AAGCTCAAAG CTGTGTTTGA CTGTGTGGTG AACTCCCTGA AGAACGTCCT CAACATCCTG
    ATCGTCTACA TGCTCTTCAT GTTCATATTT GCTGTCATCG CGGTACAGCT CTTCAAGGGG
    AAGTTTTTCT ACTGCACCGA CGAGTCCAAG GAGCTGGAGC GGGACTGCAG GGGCCAGTAT
    TTGGACTACG AGAAGGAGGA GGTGGAAGCT CAGCCACGGC AGTGGAAGAA ATACGACTTC
    CACTATGACA ACGTGCTGTG GGCCCTGCTA ACGCTGTTCA CTGTGTCCAC GGGCGAAGGG
    TGGCCCATGG TGCTGAAACA CTCGGTGGAT GCCACCTACG AGGAGCAGGG CCCCAGCCCC
    GGGTTCCGCA TGGAGCTGTC CATCTTCTAT GTGGTCTACT TCGTGGTCTT CCCCTTCTTC
    TTTGTGAACA TCTTTGTGGC CTTGATCATC ATCACCTTCC AGGAGCAGGG GGACAAAGTC
    ATGTCTGAGT GCAGCTTGGA GAAGAACGAG AGGGCTTGTA TCGACTTCGC CATTAGCGCC
    AAACCCCTGA CGCGGTACAT GCCCCAGAAC AAGCAGTCGT TCCAGTACAA GACGTGGACG
    TTCGTGGTCT CACCACCCTT TGAGTATTTC ATCATGGCCA TGATAGCCCT CAACACAGTG
    GTGCTGATGA TGAAGTTCTA CGATGCGCCC TACGAGTACG AACTGATGTT GAAGTGTCTG
    AACATCGTGT TCACGTCCAT GTTCTCCATG GAGTGTGTGC TCAAGGTCAT CGCCTTTGGA
    GTGCTGAACT ATTTCAGAGA CGCCTGGAAT GTCTTTGACT TTGTCACTGT GTTGGGAAGT
    ATTACTGATA TTTTAGTAAC AGAGATTGCG AACAACTTCA TCAACCTCAG CTTCCTCCGC
    CTCTTCCGTG CTGCCCGACT CATCAAGCTG CTCCGCCAAG GCTACACCAT CCGCATTCTG
    CTCTGGACCT TCGTCCAGTC CTTCAAGGCC CTGCCCTATG TGTGCCTGCT CATCGCCATG
    CTGTTCTTCA TCTACGCCAT CATCGGCATG CAGGTGTTTG GAAACATTGC TCTGGATGAC
    GACACCAGCA TCAACCGTCA CAATAACTTT CGGACATTTC TTCAGGCCTT GATGCTGCTC
    TTCAGGAGTG CCACCGGGGA GGCCTGGCAT GAGATCATGC TGTCCTGCCT CAGCAGTCGG
    GCCTGTGATG AGCTCGCCAA TGCCACGGAG TGTGGGAGTG ACTTCGCCTA CTTCTACTTT
    GTCTCCTTCA TCTTCCTTTG CTCCTTTCTG ATGTTGAACC TCTTTGTGGC TGTGATCATG
    GACAATTTTG AGTACCTTAC GCGGGACTCT TCCATCCTAG GGCCTCACCA CCTGGACGAA
    TTCATCCGGG TCTGGGCCGA GTACGACCCG GCTGCGTGTG GGCGCATCAG TTACAATGAC
    ATGTTTGAGA TGCTGAAACA CATGTCCCCG CCCCTGGGGC TGGGGAAGAA ATGCCCTGCT
    CGTGTTGCGT ACAAGCGCCT GGTTCGTATG AACATGCCCA TCTCCAATGA CGACATGACC
    GTCCACTTCA CATCCACGCT GATGGCCCTC ATCCGGACTG CATTGGAGAT CAAGCTGGCT
    CCAGCTGGCA CGAAGCAGCA TCAGTGTGAC GCAGAGCTGA GGAAGGAGAT CTCCTCCGTG
    TGGGCCAATC TGCCCCAGAA GACCCTGGAC TTACTGGTGC CACCCCATAA ACCCGACGAG
    ATGACGGTGG GGAAGGTGTA CGCAGCTCTG ATGATATTCG ACTTCTACAA ACAGAACAAA
    ACCACCAGAG ACCAGATTCA CCAAGCTCCT GGAGGCCTGG CCCAGATGGG CCCCGTGTCC
    CTGTTCCACC CCCTGAAGGC CACGCTGGAG CAGACTCAGC CGGCTGTGCT CCGAGGAGCC
    CGCGTTTTCC TTCGGCAGAA GAGCTCGGCC TCCCTCAGCA ACGGTGGGGC AGTACAAACC
    CAAGAGGGCG GCATCAAGGA GTCTGTCTCC TGGGGCACTC AGAGGACCCA GGAGGTCCCC
    TGCGAGGTGA GGACACCCCT GGAGCGTGGC CACTCCACAG AGATCCCGGT GCAGACAGGA
    AAACCGGCTG TGGACGTCCA GATGCAGAGC ATGGTCCTGA GAGGCCCTGA CGGGGAGCCC
    CAACCTGGGC TGGAGAGCCA GGGCCGAGCA GCTTCCATGC CCCGCCTGGC AGCAGAGACT
    CAGCCACCCC CAGATGCCAG CCCCATGAAG CGCTCCATCT CCACACTGGC ACCCCAGAGG
    CCCCATGTGG CTCATCTGTG CACCGCGGCC CTGGACCGCG CTCCAGCCAG CCAGGCCGCC
    CCCCCCCACC ACCACCGCTG CCATCGCCGC AGGGACCGGA AGCAGAAGTC CCTGGAGAAG
    GGGCCCGGCC TGTCTGCAGA CACAGATGGC GCGCCCTACA GCACCGCGGG GCCGGGCCCC
    CCGCCGCCCC CAGGGGATGG GGCAGCAGGC TGCCGGCGGG AGCGCAGGCA GGAGCGGGGC
    CGGTCCCAGG AGCGGAGGCA GCCCTCCTCC TCTTCCTCTG AGAAGCAGCG CTTCTACTCC
    TGTGACCGCT TTGGGGGCCG TGAGCCCCCG CAGCCCAAGC CCTCTCTCAG CAGCCATCCC
    ACGTCGCCCA CGGCCGGGCA GGAGCCAGGA CCCCCGAGAC AGGGTAGCGG CTCCGTGAAC
    GGGAGCCCCC TGCTGTCGAC ATCTGGTGCT AGCACCCCTG GCCGCGGCGG GCGGAGGCAG
    CTCCCCCAGA CCCCGCTGAC CCCCCGTCCC AGCATCACCT ATAAGACGGC CAACTCCTCA
    CCTGTCCACT TTGCCGGGGC TCAGACCAGC CTCCCTGCCT TTTCCCCCGG CCGGCTTAGC
    CGTGGCCTTT CGGAACACAA CGCCCTGCTC CAGAGAGACC CCCTCAGCCA GCCGCTGGCC
    CCCAGCTCTC GGATTGGCTC CGACCCTTAC CTGGGGCAGC GTCTGGACAG TGAGGCTGCG
    GCCCGCACCC AGCTTGAGGA CACGCTCACC TTCGAGGAGG CCGTGGCCAC CAACTCGGGC
    CGCTCCTCCC GGACTTCCTA CGTGTCCTCC CTGACCTCGC AGTCCCACCC CCTCCGCCGG
    GTGCCCAACG GCTACCACTG CACTCTGGGG CTCAGCTCGG GCGGGGGCGG CCGGGGGCGG
    CACAGCTACC ACCACCCTGA CCAAGACCAC TGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854109.1
    CDS1..6999
    Translation

    Target ORF information:

    RefSeq Version XM_024998341.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998341.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGCAGTGTT GAAGAGAGCA
    GCCACCAAGA AGAGCCGAAA CGACCTGATC CACGCGGAGG AGGGAGAGGA CCGCTTTGCA
    GACCTCTGTG CTGTGGGGTC CCCCTTCGCC CGAGCCAGCC TCAAGAGTGG GAAGACGGAG
    AGCTCGTCAT ACTTCCGCAG GAAGGAGAAG ATGTTCCGGT TCTTCATCCG GCGCCTGGTG
    AAGGCGCAGA GCTTCTACTG GGTGGTGCTG TGTGTGGTGG CCCTGAACAC GCTGTGCGTG
    GCCATGGTGC ACTACCAGCA GCCCCAGCGG CTCACCACGG CGCTGTACTT TGCAGAGTTT
    GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGG
    AGCTACTTCC GGTCCTCCTT CAACTGCTTC GATTTCGGGG TCATTGTGGG GAGCATTTTC
    GAAGTGGTCT GGGCGGCCAT CAAGCCAGGA ACCTCCTTCG GCATCAGTGT GCTGCGGGCG
    CTGCGGCTGC TGAGAATCTT CAAAGTCACC AAGTACTGGA GCTCCCTGCG GAACCTGGTG
    GTGTCCCTGC TCAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCCTGCT CTTCCTCTTC
    ATCGTGGTCT TCGCTCTGCT GGGCATGCAG CTCTTCGGGG GACAGTTCAA CTTCCAGGAT
    GAGACTCCGA CAACCAACTT TGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC
    CTGACCGGGG AGGACTGGAA TGCGGTGATG TACCATGGGA TCGAATCACA AGGTGGAGTC
    AGCAAGGGCA TGTTCTCGTC TGTGTACTTC ATTGTCCTGA CGCTGTTTGG GAACTACACC
    CTGCTGAACG TCTTCCTGGC CATCGCTGTG GACAACCTCG CCAATGCTCA GGAGCTGACC
    AAGGACGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA
    GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATTGCTGC CAGGCAGCAG
    AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTGCG GCTGCAGAAC
    CTGCGGGCCA GCTGTGAAGC GCTGTATAGC GAGATGGACC CCGAGGAGCG CCTGCGCTTC
    GCCACTTCCC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTTGTGGTG
    GAGCCAGGCC GGGATGGCGC GCGGGGGCCT GCGGGGGGCA AGGCCCGGCC CGAGGGCGGG
    GAGGCCGGCG AGGGTGCCGA CCCGCCGCGC AGGCACCACC GGCACCGCGA CAGGGACAGG
    GACAAGGCCG CAGCCCCGGC GGGGGAGCCA GACAGGGCGG ACGCCCCGAA GGCGGAGGGC
    GGGGAGCCGG GGGCCCGGGA GGAGCGGGCG CGACCGCGCC GCAGCCGCAG CAAGGAGGCC
    GCAGGCCCCC GCGAGGCCAG GAGCGAGCGA GGCCGCGGGC TGGGCCCGGA CGGCGGCCGG
    CGCCACCACC GGCGCGGCTC CCCGGAGGAG GCCGCGGAGC GCGAGCCCCG GCGCCACCGC
    GCGCACCGGC ACGCGCCGGA GCCGAGCAGG GAGGGCGCGC CCGGGAGCAA GGGCGAGCGG
    CGAGCGCGGC ACCGCGGCGG CTCCCGGGCC GGGCCCCGGG AGGCCGAGAG CGGCGAGGAG
    CCCTCCCGGC GGCACCGAGC GCGACACAAG GCGCCGCCCG CACACGAGGA GGCGGAGAAG
    GAGGCCGAGG CGGAGGACAG GGACAAGGAG CCCCGAAACC ACCAGCCCAG GGAGTCGCAC
    TGTGACCTGG AGGCCAGTGT GGTCATTGGT GTGGGCCCTG TGCATGCCCT GCCCAGCACT
    TGTCTAGAGA AGGTGGAAGA GCAGCCAGAG GATGCAGACA ATCAGCGGAA TGTGACTCGC
    ATGGGCAGTC AGCCCTCAGA TCTGAGCACC GCTGTGCGTA TCCCAGTGAC GCTGACCGGG
    CCTCCCGGGG AGACCACGGT TGTTCCCAGT GGTAACGTGG ACCTGGAAAG CCAAGCAGAA
    GGAAAGAAGG AGGTGGAAGC TGATGACGTG ATGAGGAGTG GCCCCCGACC CATTGTCCCG
    TACAGCTCCA TGTTCTGCTT GAGCCCCACC AACCTGCTCC GCCGCTTCTG CCACTACATT
    GTGACCATGC GGTACTTCGA GATGGTCATT CTCGTGGTCA TTGCCCTGAG TAGCATTGCC
    CTGGCTGCGG AGGATCCTGT GCAGACAGAC TCGCCCAGGA ACAACGTGCT GAAGTACATG
    GACTACATCT TCACAGGTGT CTTCACCTTT GAGATGGTGA TAAAGATGAT CGATTTGGGA
    CTGCTGCTGC ACCCCGGTGC CTACTTCCGG GATCTGTGGA ACATTCTGGA CTTCATTGTG
    GTCAGTGGGG CCCTGGTGGC GTTTGCCTTC TCAGGCTCCA AAGGGAAAGA CATCAGCACC
    ATCAAGTCCC TGAGGGTCCT GCGTGTGCTG AGGCCACTCA AGACCATCAA GCGGCTGCCC
    AAGCTCAAAG CTGTGTTTGA CTGTGTGGTG AACTCCCTGA AGAACGTCCT CAACATCCTG
    ATCGTCTACA TGCTCTTCAT GTTCATATTT GCTGTCATCG CGGTACAGCT CTTCAAGGGG
    AAGTTTTTCT ACTGCACCGA CGAGTCCAAG GAGCTGGAGC GGGACTGCAG GGGCCAGTAT
    TTGGACTACG AGAAGGAGGA GGTGGAAGCT CAGCCACGGC AGTGGAAGAA ATACGACTTC
    CACTATGACA ACGTGCTGTG GGCCCTGCTA ACGCTGTTCA CTGTGTCCAC GGGCGAAGGG
    TGGCCCATGG TGCTGAAACA CTCGGTGGAT GCCACCTACG AGGAGCAGGG CCCCAGCCCC
    GGGTTCCGCA TGGAGCTGTC CATCTTCTAT GTGGTCTACT TCGTGGTCTT CCCCTTCTTC
    TTTGTGAACA TCTTTGTGGC CTTGATCATC ATCACCTTCC AGGAGCAGGG GGACAAAGTC
    ATGTCTGAGT GCAGCTTGGA GAAGAACGAG AGGGCTTGTA TCGACTTCGC CATTAGCGCC
    AAACCCCTGA CGCGGTACAT GCCCCAGAAC AAGCAGTCGT TCCAGTACAA GACGTGGACG
    TTCGTGGTCT CACCACCCTT TGAGTATTTC ATCATGGCCA TGATAGCCCT CAACACAGTG
    GTGCTGATGA TGAAGTTCTA CGATGCGCCC TACGAGTACG AACTGATGTT GAAGTGTCTG
    AACATCGTGT TCACGTCCAT GTTCTCCATG GAGTGTGTGC TCAAGGTCAT CGCCTTTGGA
    GTGCTGAACT ATTTCAGAGA CGCCTGGAAT GTCTTTGACT TTGTCACTGT GTTGGGAAGT
    ATTACTGATA TTTTAGTAAC AGAGATTGCG AACAACTTCA TCAACCTCAG CTTCCTCCGC
    CTCTTCCGTG CTGCCCGACT CATCAAGCTG CTCCGCCAAG GCTACACCAT CCGCATTCTG
    CTCTGGACCT TCGTCCAGTC CTTCAAGGCC CTGCCCTATG TGTGCCTGCT CATCGCCATG
    CTGTTCTTCA TCTACGCCAT CATCGGCATG CAGGTGTTTG GAAACATTGC TCTGGATGAC
    GACACCAGCA TCAACCGTCA CAATAACTTT CGGACATTTC TTCAGGCCTT GATGCTGCTC
    TTCAGGAGTG CCACCGGGGA GGCCTGGCAT GAGATCATGC TGTCCTGCCT CAGCAGTCGG
    GCCTGTGATG AGCTCGCCAA TGCCACGGAG TGTGGGAGTG ACTTCGCCTA CTTCTACTTT
    GTCTCCTTCA TCTTCCTTTG CTCCTTTCTG ATGTTGAACC TCTTTGTGGC TGTGATCATG
    GACAATTTTG AGTACCTTAC GCGGGACTCT TCCATCCTAG GGCCTCACCA CCTGGACGAA
    TTCATCCGGG TCTGGGCCGA GTACGACCCG GCTGCGTGTG GGCGCATCAG TTACAATGAC
    ATGTTTGAGA TGCTGAAACA CATGTCCCCG CCCCTGGGGC TGGGGAAGAA ATGCCCTGCT
    CGTGTTGCGT ACAAGCGCCT GGTTCGTATG AACATGCCCA TCTCCAATGA CGACATGACC
    GTCCACTTCA CATCCACGCT GATGGCCCTC ATCCGGACTG CATTGGAGAT CAAGCTGGCT
    CCAGCTGGCA CGAAGCAGCA TCAGTGTGAC GCAGAGCTGA GGAAGGAGAT CTCCTCCGTG
    TGGGCCAATC TGCCCCAGAA GACCCTGGAC TTACTGGTGC CACCCCATAA ACCCGACGAG
    ATGACGGTGG GGAAGGTGTA CGCAGCTCTG ATGATATTCG ACTTCTACAA ACAGAACAAA
    ACCACCAGAG ACCAGATTCA CCAAGCTCCT GGAGGCCTGG CCCAGATGGG CCCCGTGTCC
    CTGTTCCACC CCCTGAAGGC CACGCTGGAG CAGACTCAGC CGGCTGTGCT CCGAGGAGCC
    CGCGTTTTCC TTCGGCAGAA GAGCTCGGCC TCCCTCAGCA ACGGTGGGGC AGTACAAACC
    CAAGAGGGCG GCATCAAGGA GTCTGTCTCC TGGGGCACTC AGAGGACCCA GGAGGTCCCC
    TGCGAGGTGA GGACACCCCT GGAGCGTGGC CACTCCACAG AGATCCCGGT GCAGACAGGA
    AAACCGGCTG TGGACGTCCA GATGCAGAGC ATGGTCCTGA GAGGCCCTGA CGGGGAGCCC
    CAACCTGGGC TGGAGAGCCA GGGCCGAGCA GCTTCCATGC CCCGCCTGGC AGCAGAGACT
    CAGCCACCCC CAGATGCCAG CCCCATGAAG CGCTCCATCT CCACACTGGC ACCCCAGAGG
    CCCCATGTGG CTCATCTGTG CACCGCGGCC CTGGACCGCG CTCCAGCCAG CCAGGCCGCC
    CCCCCCCACC ACCACCGCTG CCATCGCCGC AGGGACCGGA AGCAGAAGTC CCTGGAGAAG
    GGGCCCGGCC TGTCTGCAGA CACAGATGGC GCGCCCTACA GCACCGCGGG GCCGGGCCCC
    CCGCCGCCCC CAGGGGATGG GGCAGCAGGC TGCCGGCGGG AGCGCAGGCA GGAGCGGGGC
    CGGTCCCAGG AGCGGAGGCA GCCCTCCTCC TCTTCCTCTG AGAAGCAGCG CTTCTACTCC
    TGTGACCGCT TTGGGGGCCG TGAGCCCCCG CAGCCCAAGC CCTCTCTCAG CAGCCATCCC
    ACGTCGCCCA CGGCCGGGCA GGAGCCAGGA CCCCCGAGAC AGGGTAGCGG CTCCGTGAAC
    GGGAGCCCCC TGCTGTCGAC ATCTGGTGCT AGCACCCCTG GCCGCGGCGG GCGGAGGCAG
    CTCCCCCAGA CCCCGCTGAC CCCCCGTCCC AGCATCACCT ATAAGACGGC CAACTCCTCA
    CCTGTCCACT TTGCCGGGGC TCAGACCAGC CTCCCTGCCT TTTCCCCCGG CCGGCTTAGC
    CGTGGCCTTT CGGAACACAA CGCCCTGCTC CAGAGAGACC CCCTCAGCCA GCCGCTGGCC
    CCCAGCTCTC GGATTGGCTC CGACCCTTAC CTGGGGCAGC GTCTGGACAG TGAGGCTGCG
    GCCCGCACCC AGCTTGAGGA CACGCTCACC TTCGAGGAGG CCGTGGCCAC CAACTCGGGC
    CGCTCCTCCC GGACTTCCTA CGTGTCCTCC CTGACCTCGC AGTCCCACCC CCTCCGCCGG
    GTGCCCAACG GCTACCACTG CACTCTGGGG CTCAGCTCGG GCGGGGGCGG CCGGGGGCGG
    CACAGCTACC ACCACCCTGA CCAAGACCAC TGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220145
    Clone ID Related Accession (Same CDS sequence) XM_024998342.1
    Accession Version XM_024998342.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGCAGTGTT GAAGAGAGCA
    GCCACCAAGA AGAGCCGAAA CGACCTGATC CACGCGGAGG AGGGAGAGGA CCGCTTTGCA
    GACCTCTGTG CTGTGGGGTC CCCCTTCGCC CGAGCCAGCC TCAAGAGTGG GAAGACGGAG
    AGCTCGTCAT ACTTCCGCAG GAAGGAGAAG ATGTTCCGGT TCTTCATCCG GCGCCTGGTG
    AAGGCGCAGA GCTTCTACTG GGTGGTGCTG TGTGTGGTGG CCCTGAACAC GCTGTGCGTG
    GCCATGGTGC ACTACCAGCA GCCCCAGCGG CTCACCACGG CGCTGTACTT TGCAGAGTTT
    GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGG
    AGCTACTTCC GGTCCTCCTT CAACTGCTTC GATTTCGGGG TCATTGTGGG GAGCATTTTC
    GAAGTGGTCT GGGCGGCCAT CAAGCCAGGA ACCTCCTTCG GCATCAGTGT GCTGCGGGCG
    CTGCGGCTGC TGAGAATCTT CAAAGTCACC AAGTACTGGA GCTCCCTGCG GAACCTGGTG
    GTGTCCCTGC TCAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCCTGCT CTTCCTCTTC
    ATCGTGGTCT TCGCTCTGCT GGGCATGCAG CTCTTCGGGG GACAGTTCAA CTTCCAGGAT
    GAGACTCCGA CAACCAACTT TGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC
    CTGACCGGGG AGGACTGGAA TGCGGTGATG TACCATGGGA TCGAATCACA AGGTGGAGTC
    AGCAAGGGCA TGTTCTCGTC TGTGTACTTC ATTGTCCTGA CGCTGTTTGG GAACTACACC
    CTGCTGAACG TCTTCCTGGC CATCGCTGTG GACAACCTCG CCAATGCTCA GGAGCTGACC
    AAGGACGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA
    GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATTGCTGC CAGGCAGCAG
    AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTGCG GCTGCAGAAC
    CTGCGGGCCA GCTGTGAAGC GCTGTATAGC GAGATGGACC CCGAGGAGCG CCTGCGCTTC
    GCCACTTCCC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTTGTGGTG
    GAGCCAGGCC GGGATGGCGC GCGGGGGCCT GCGGGGGGCA AGGCCCGGCC CGAGGGCGGG
    GAGGCCGGCG AGGGTGCCGA CCCGCCGCGC AGGCACCACC GGCACCGCGA CAGGGACAGG
    GACAAGGCCG CAGCCCCGGC GGGGGAGCCA GACAGGGCGG ACGCCCCGAA GGCGGAGGGC
    GGGGAGCCGG GGGCCCGGGA GGAGCGGGCG CGACCGCGCC GCAGCCGCAG CAAGGAGGCC
    GCAGGCCCCC GCGAGGCCAG GAGCGAGCGA GGCCGCGGGC TGGGCCCGGA CGGCGGCCGG
    CGCCACCACC GGCGCGGCTC CCCGGAGGAG GCCGCGGAGC GCGAGCCCCG GCGCCACCGC
    GCGCACCGGC ACGCGCCGGA GCCGAGCAGG GAGGGCGCGC CCGGGAGCAA GGGCGAGCGG
    CGAGCGCGGC ACCGCGGCGG CTCCCGGGCC GGGCCCCGGG AGGCCGAGAG CGGCGAGGAG
    CCCTCCCGGC GGCACCGAGC GCGACACAAG GCGCCGCCCG CACACGAGGA GGCGGAGAAG
    GAGGCCGAGG CGGAGGACAG GGACAAGGAG CCCCGAAACC ACCAGCCCAG GGAGTCGCAC
    TGTGACCTGG AGGCCAGTGT GGTCATTGGT GTGGGCCCTG TGCATGCCCT GCCCAGCACT
    TGTCTAGAGA AGGTGGAAGA GCAGCCAGAG GATGCAGACA ATCAGCGGAA TGTGACTCGC
    ATGGGCAGTC AGCCCTCAGA TCTGAGCACC GCTGTGCGTA TCCCAGTGAC GCTGACCGGG
    CCTCCCGGGG AGACCACGGT TGTTCCCAGT GGTAACGTGG ACCTGGAAAG CCAAGCAGAA
    GGAAAGAAGG AGGTGGAAGC TGATGACGTG ATGAGGAGTG GCCCCCGACC CATTGTCCCG
    TACAGCTCCA TGTTCTGCTT GAGCCCCACC AACCTGCTCC GCCGCTTCTG CCACTACATT
    GTGACCATGC GGTACTTCGA GATGGTCATT CTCGTGGTCA TTGCCCTGAG TAGCATTGCC
    CTGGCTGCGG AGGATCCTGT GCAGACAGAC TCGCCCAGGA ACAACGTGCT GAAGTACATG
    GACTACATCT TCACAGGTGT CTTCACCTTT GAGATGGTGA TAAAGATGAT CGATTTGGGA
    CTGCTGCTGC ACCCCGGTGC CTACTTCCGG GATCTGTGGA ACATTCTGGA CTTCATTGTG
    GTCAGTGGGG CCCTGGTGGC GTTTGCCTTC TCGAGCTTCA TGGGAGGCTC CAAAGGGAAA
    GACATCAGCA CCATCAAGTC CCTGAGGGTC CTGCGTGTGC TGAGGCCACT CAAGACCATC
    AAGCGGCTGC CCAAGCTCAA ACTCTTCAAG GGGAAGTTTT TCTACTGCAC CGACGAGTCC
    AAGGAGCTGG AGCGGGACTG CAGGGGCCAG TATTTGGACT ACGAGAAGGA GGAGGTGGAA
    GCTCAGCCAC GGCAGTGGAA GAAATACGAC TTCCACTATG ACAACGTGCT GTGGGCCCTG
    CTAACGCTGT TCACTGTGTC CACGGGCGAA GGGTGGCCCA TGGTGCTGAA ACACTCGGTG
    GATGCCACCT ACGAGGAGCA GGGCCCCAGC CCCGGGTTCC GCATGGAGCT GTCCATCTTC
    TATGTGGTCT ACTTCGTGGT CTTCCCCTTC TTCTTTGTGA ACATCTTTGT GGCCTTGATC
    ATCATCACCT TCCAGGAGCA GGGGGACAAA GTCATGTCTG AGTGCAGCTT GGAGAAGAAC
    GAGAGGGCTT GTATCGACTT CGCCATTAGC GCCAAACCCC TGACGCGGTA CATGCCCCAG
    AACAAGCAGT CGTTCCAGTA CAAGACGTGG ACGTTCGTGG TCTCACCACC CTTTGAGTAT
    TTCATCATGG CCATGATAGC CCTCAACACA GTGGTGCTGA TGATGAAGTT CTACGATGCG
    CCCTACGAGT ACGAACTGAT GTTGAAGTGT CTGAACATCG TGTTCACGTC CATGTTCTCC
    ATGGAGTGTG TGCTCAAGGT CATCGCCTTT GGAGTGCTGA ACTATTTCAG AGACGCCTGG
    AATGTCTTTG ACTTTGTCAC TGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT
    GCGGAAACGA ACAACTTCAT CAACCTCAGC TTCCTCCGCC TCTTCCGTGC TGCCCGACTC
    ATCAAGCTGC TCCGCCAAGG CTACACCATC CGCATTCTGC TCTGGACCTT CGTCCAGTCC
    TTCAAGGCCC TGCCCTATGT GTGCCTGCTC ATCGCCATGC TGTTCTTCAT CTACGCCATC
    ATCGGCATGC AGGTGTTTGG AAACATTGCT CTGGATGACG ACACCAGCAT CAACCGTCAC
    AATAACTTTC GGACATTTCT TCAGGCCTTG ATGCTGCTCT TCAGGAGTGC CACCGGGGAG
    GCCTGGCATG AGATCATGCT GTCCTGCCTC AGCAGTCGGG CCTGTGATGA GCTCGCCAAT
    GCCACGGAGT GTGGGAGTGA CTTCGCCTAC TTCTACTTTG TCTCCTTCAT CTTCCTTTGC
    TCCTTTCTGA TGTTGAACCT CTTTGTGGCT GTGATCATGG ACAATTTTGA GTACCTTACG
    CGGGACTCTT CCATCCTAGG GCCTCACCAC CTGGACGAAT TCATCCGGGT CTGGGCCGAG
    TACGACCCGG CTGCGTGTGG GCGCATCAGT TACAATGACA TGTTTGAGAT GCTGAAACAC
    ATGTCCCCGC CCCTGGGGCT GGGGAAGAAA TGCCCTGCTC GTGTTGCGTA CAAGCGCCTG
    GTTCGTATGA ACATGCCCAT CTCCAATGAC GACATGACCG TCCACTTCAC ATCCACGCTG
    ATGGCCCTCA TCCGGACTGC ATTGGAGATC AAGCTGGCTC CAGCTGGCAC GAAGCAGCAT
    CAGTGTGACG CAGAGCTGAG GAAGGAGATC TCCTCCGTGT GGGCCAATCT GCCCCAGAAG
    ACCCTGGACT TACTGGTGCC ACCCCATAAA CCCGACGAGA TGACGGTGGG GAAGGTGTAC
    GCAGCTCTGA TGATATTCGA CTTCTACAAA CAGAACAAAA CCACCAGAGA CCAGATTCAC
    CAAGCTCCTG GAGGCCTGGC CCAGATGGGC CCCGTGTCCC TGTTCCACCC CCTGAAGGCC
    ACGCTGGAGC AGACTCAGCC GGCTGTGCTC CGAGGAGCCC GCGTTTTCCT TCGGCAGAAG
    AGCTCGGCCT CCCTCAGCAA CGGTGGGGCA GTACAAACCC AAGAGGGCGG CATCAAGGAG
    TCTGTCTCCT GGGGCACTCA GAGGACCCAG GAGGTCCCCT GCGAGGTGAG GACACCCCTG
    GAGCGTGGCC ACTCCACAGA GATCCCGGTG CAGACAGGAA AACCGGCTGT GGACGTCCAG
    ATGCAGAGCA TGGTCCTGAG AGGCCCTGAC GGGGAGCCCC AACCTGGGCT GGAGAGCCAG
    GGCCGAGCAG CTTCCATGCC CCGCCTGGCA GCAGAGACTC AGCCACCCCC AGATGCCAGC
    CCCATGAAGC GCTCCATCTC CACACTGGCA CCCCAGAGGC CCCATGTGGC TCATCTGTGC
    ACCGCGGCCC TGGACCGCGC TCCAGCCAGC CAGGCCGCCC CCCCCCACCA CCACCGCTGC
    CATCGCCGCA GGGACCGGAA GCAGAAGTCC CTGGAGAAGG GGCCCGGCCT GTCTGCAGAC
    ACAGATGGCG CGCCCTACAG CACCGCGGGG CCGGGCCCCC CGCCGCCCCC AGGGGATGGG
    GCAGCAGGCT GCCGGCGGGA GCGCAGGCAG GAGCGGGGCC GGTCCCAGGA GCGGAGGCAG
    CCCTCCTCCT CTTCCTCTGA GAAGCAGCGC TTCTACTCCT GTGACCGCTT TGGGGGCCGT
    GAGCCCCCGC AGCCCAAGCC CTCTCTCAGC AGCCATCCCA CGTCGCCCAC GGCCGGGCAG
    GAGCCAGGAC CCCCGAGACA GGGTAGCGGC TCCGTGAACG GGAGCCCCCT GCTGTCGACA
    TCTGGTGCTA GCACCCCTGG CCGCGGCGGG CGGAGGCAGC TCCCCCAGAC CCCGCTGACC
    CCCCGTCCCA GCATCACCTA TAAGACGGCC AACTCCTCAC CTGTCCACTT TGCCGGGGCT
    CAGACCAGCC TCCCTGCCTT TTCCCCCGGC CGGCTTAGCC GTGGCCTTTC GGAACACAAC
    GCCCTGCTCC AGAGAGACCC CCTCAGCCAG CCGCTGGCCC CCAGCTCTCG GATTGGCTCC
    GACCCTTACC TGGGGCAGCG TCTGGACAGT GAGGCTGCGG CCCGCACCCA GCTTGAGGAC
    ACGCTCACCT TCGAGGAGGC CGTGGCCACC AACTCGGGCC GCTCCTCCCG GACTTCCTAC
    GTGTCCTCCC TGACCTCGCA GTCCCACCCC CTCCGCCGGG TGCCCAACGG CTACCACTGC
    ACTCTGGGGC TCAGCTCGGG CGGGGGCGGC CGGGGGCGGC ACAGCTACCA CCACCCTGAC
    CAAGACCACT GGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854110.1
    CDS140..7057
    Translation

    Target ORF information:

    RefSeq Version XM_024998342.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998342.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGCAGTGTT GAAGAGAGCA
    GCCACCAAGA AGAGCCGAAA CGACCTGATC CACGCGGAGG AGGGAGAGGA CCGCTTTGCA
    GACCTCTGTG CTGTGGGGTC CCCCTTCGCC CGAGCCAGCC TCAAGAGTGG GAAGACGGAG
    AGCTCGTCAT ACTTCCGCAG GAAGGAGAAG ATGTTCCGGT TCTTCATCCG GCGCCTGGTG
    AAGGCGCAGA GCTTCTACTG GGTGGTGCTG TGTGTGGTGG CCCTGAACAC GCTGTGCGTG
    GCCATGGTGC ACTACCAGCA GCCCCAGCGG CTCACCACGG CGCTGTACTT TGCAGAGTTT
    GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGG
    AGCTACTTCC GGTCCTCCTT CAACTGCTTC GATTTCGGGG TCATTGTGGG GAGCATTTTC
    GAAGTGGTCT GGGCGGCCAT CAAGCCAGGA ACCTCCTTCG GCATCAGTGT GCTGCGGGCG
    CTGCGGCTGC TGAGAATCTT CAAAGTCACC AAGTACTGGA GCTCCCTGCG GAACCTGGTG
    GTGTCCCTGC TCAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCCTGCT CTTCCTCTTC
    ATCGTGGTCT TCGCTCTGCT GGGCATGCAG CTCTTCGGGG GACAGTTCAA CTTCCAGGAT
    GAGACTCCGA CAACCAACTT TGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC
    CTGACCGGGG AGGACTGGAA TGCGGTGATG TACCATGGGA TCGAATCACA AGGTGGAGTC
    AGCAAGGGCA TGTTCTCGTC TGTGTACTTC ATTGTCCTGA CGCTGTTTGG GAACTACACC
    CTGCTGAACG TCTTCCTGGC CATCGCTGTG GACAACCTCG CCAATGCTCA GGAGCTGACC
    AAGGACGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA
    GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATTGCTGC CAGGCAGCAG
    AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTGCG GCTGCAGAAC
    CTGCGGGCCA GCTGTGAAGC GCTGTATAGC GAGATGGACC CCGAGGAGCG CCTGCGCTTC
    GCCACTTCCC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTTGTGGTG
    GAGCCAGGCC GGGATGGCGC GCGGGGGCCT GCGGGGGGCA AGGCCCGGCC CGAGGGCGGG
    GAGGCCGGCG AGGGTGCCGA CCCGCCGCGC AGGCACCACC GGCACCGCGA CAGGGACAGG
    GACAAGGCCG CAGCCCCGGC GGGGGAGCCA GACAGGGCGG ACGCCCCGAA GGCGGAGGGC
    GGGGAGCCGG GGGCCCGGGA GGAGCGGGCG CGACCGCGCC GCAGCCGCAG CAAGGAGGCC
    GCAGGCCCCC GCGAGGCCAG GAGCGAGCGA GGCCGCGGGC TGGGCCCGGA CGGCGGCCGG
    CGCCACCACC GGCGCGGCTC CCCGGAGGAG GCCGCGGAGC GCGAGCCCCG GCGCCACCGC
    GCGCACCGGC ACGCGCCGGA GCCGAGCAGG GAGGGCGCGC CCGGGAGCAA GGGCGAGCGG
    CGAGCGCGGC ACCGCGGCGG CTCCCGGGCC GGGCCCCGGG AGGCCGAGAG CGGCGAGGAG
    CCCTCCCGGC GGCACCGAGC GCGACACAAG GCGCCGCCCG CACACGAGGA GGCGGAGAAG
    GAGGCCGAGG CGGAGGACAG GGACAAGGAG CCCCGAAACC ACCAGCCCAG GGAGTCGCAC
    TGTGACCTGG AGGCCAGTGT GGTCATTGGT GTGGGCCCTG TGCATGCCCT GCCCAGCACT
    TGTCTAGAGA AGGTGGAAGA GCAGCCAGAG GATGCAGACA ATCAGCGGAA TGTGACTCGC
    ATGGGCAGTC AGCCCTCAGA TCTGAGCACC GCTGTGCGTA TCCCAGTGAC GCTGACCGGG
    CCTCCCGGGG AGACCACGGT TGTTCCCAGT GGTAACGTGG ACCTGGAAAG CCAAGCAGAA
    GGAAAGAAGG AGGTGGAAGC TGATGACGTG ATGAGGAGTG GCCCCCGACC CATTGTCCCG
    TACAGCTCCA TGTTCTGCTT GAGCCCCACC AACCTGCTCC GCCGCTTCTG CCACTACATT
    GTGACCATGC GGTACTTCGA GATGGTCATT CTCGTGGTCA TTGCCCTGAG TAGCATTGCC
    CTGGCTGCGG AGGATCCTGT GCAGACAGAC TCGCCCAGGA ACAACGTGCT GAAGTACATG
    GACTACATCT TCACAGGTGT CTTCACCTTT GAGATGGTGA TAAAGATGAT CGATTTGGGA
    CTGCTGCTGC ACCCCGGTGC CTACTTCCGG GATCTGTGGA ACATTCTGGA CTTCATTGTG
    GTCAGTGGGG CCCTGGTGGC GTTTGCCTTC TCGAGCTTCA TGGGAGGCTC CAAAGGGAAA
    GACATCAGCA CCATCAAGTC CCTGAGGGTC CTGCGTGTGC TGAGGCCACT CAAGACCATC
    AAGCGGCTGC CCAAGCTCAA ACTCTTCAAG GGGAAGTTTT TCTACTGCAC CGACGAGTCC
    AAGGAGCTGG AGCGGGACTG CAGGGGCCAG TATTTGGACT ACGAGAAGGA GGAGGTGGAA
    GCTCAGCCAC GGCAGTGGAA GAAATACGAC TTCCACTATG ACAACGTGCT GTGGGCCCTG
    CTAACGCTGT TCACTGTGTC CACGGGCGAA GGGTGGCCCA TGGTGCTGAA ACACTCGGTG
    GATGCCACCT ACGAGGAGCA GGGCCCCAGC CCCGGGTTCC GCATGGAGCT GTCCATCTTC
    TATGTGGTCT ACTTCGTGGT CTTCCCCTTC TTCTTTGTGA ACATCTTTGT GGCCTTGATC
    ATCATCACCT TCCAGGAGCA GGGGGACAAA GTCATGTCTG AGTGCAGCTT GGAGAAGAAC
    GAGAGGGCTT GTATCGACTT CGCCATTAGC GCCAAACCCC TGACGCGGTA CATGCCCCAG
    AACAAGCAGT CGTTCCAGTA CAAGACGTGG ACGTTCGTGG TCTCACCACC CTTTGAGTAT
    TTCATCATGG CCATGATAGC CCTCAACACA GTGGTGCTGA TGATGAAGTT CTACGATGCG
    CCCTACGAGT ACGAACTGAT GTTGAAGTGT CTGAACATCG TGTTCACGTC CATGTTCTCC
    ATGGAGTGTG TGCTCAAGGT CATCGCCTTT GGAGTGCTGA ACTATTTCAG AGACGCCTGG
    AATGTCTTTG ACTTTGTCAC TGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT
    GCGGAAACGA ACAACTTCAT CAACCTCAGC TTCCTCCGCC TCTTCCGTGC TGCCCGACTC
    ATCAAGCTGC TCCGCCAAGG CTACACCATC CGCATTCTGC TCTGGACCTT CGTCCAGTCC
    TTCAAGGCCC TGCCCTATGT GTGCCTGCTC ATCGCCATGC TGTTCTTCAT CTACGCCATC
    ATCGGCATGC AGGTGTTTGG AAACATTGCT CTGGATGACG ACACCAGCAT CAACCGTCAC
    AATAACTTTC GGACATTTCT TCAGGCCTTG ATGCTGCTCT TCAGGAGTGC CACCGGGGAG
    GCCTGGCATG AGATCATGCT GTCCTGCCTC AGCAGTCGGG CCTGTGATGA GCTCGCCAAT
    GCCACGGAGT GTGGGAGTGA CTTCGCCTAC TTCTACTTTG TCTCCTTCAT CTTCCTTTGC
    TCCTTTCTGA TGTTGAACCT CTTTGTGGCT GTGATCATGG ACAATTTTGA GTACCTTACG
    CGGGACTCTT CCATCCTAGG GCCTCACCAC CTGGACGAAT TCATCCGGGT CTGGGCCGAG
    TACGACCCGG CTGCGTGTGG GCGCATCAGT TACAATGACA TGTTTGAGAT GCTGAAACAC
    ATGTCCCCGC CCCTGGGGCT GGGGAAGAAA TGCCCTGCTC GTGTTGCGTA CAAGCGCCTG
    GTTCGTATGA ACATGCCCAT CTCCAATGAC GACATGACCG TCCACTTCAC ATCCACGCTG
    ATGGCCCTCA TCCGGACTGC ATTGGAGATC AAGCTGGCTC CAGCTGGCAC GAAGCAGCAT
    CAGTGTGACG CAGAGCTGAG GAAGGAGATC TCCTCCGTGT GGGCCAATCT GCCCCAGAAG
    ACCCTGGACT TACTGGTGCC ACCCCATAAA CCCGACGAGA TGACGGTGGG GAAGGTGTAC
    GCAGCTCTGA TGATATTCGA CTTCTACAAA CAGAACAAAA CCACCAGAGA CCAGATTCAC
    CAAGCTCCTG GAGGCCTGGC CCAGATGGGC CCCGTGTCCC TGTTCCACCC CCTGAAGGCC
    ACGCTGGAGC AGACTCAGCC GGCTGTGCTC CGAGGAGCCC GCGTTTTCCT TCGGCAGAAG
    AGCTCGGCCT CCCTCAGCAA CGGTGGGGCA GTACAAACCC AAGAGGGCGG CATCAAGGAG
    TCTGTCTCCT GGGGCACTCA GAGGACCCAG GAGGTCCCCT GCGAGGTGAG GACACCCCTG
    GAGCGTGGCC ACTCCACAGA GATCCCGGTG CAGACAGGAA AACCGGCTGT GGACGTCCAG
    ATGCAGAGCA TGGTCCTGAG AGGCCCTGAC GGGGAGCCCC AACCTGGGCT GGAGAGCCAG
    GGCCGAGCAG CTTCCATGCC CCGCCTGGCA GCAGAGACTC AGCCACCCCC AGATGCCAGC
    CCCATGAAGC GCTCCATCTC CACACTGGCA CCCCAGAGGC CCCATGTGGC TCATCTGTGC
    ACCGCGGCCC TGGACCGCGC TCCAGCCAGC CAGGCCGCCC CCCCCCACCA CCACCGCTGC
    CATCGCCGCA GGGACCGGAA GCAGAAGTCC CTGGAGAAGG GGCCCGGCCT GTCTGCAGAC
    ACAGATGGCG CGCCCTACAG CACCGCGGGG CCGGGCCCCC CGCCGCCCCC AGGGGATGGG
    GCAGCAGGCT GCCGGCGGGA GCGCAGGCAG GAGCGGGGCC GGTCCCAGGA GCGGAGGCAG
    CCCTCCTCCT CTTCCTCTGA GAAGCAGCGC TTCTACTCCT GTGACCGCTT TGGGGGCCGT
    GAGCCCCCGC AGCCCAAGCC CTCTCTCAGC AGCCATCCCA CGTCGCCCAC GGCCGGGCAG
    GAGCCAGGAC CCCCGAGACA GGGTAGCGGC TCCGTGAACG GGAGCCCCCT GCTGTCGACA
    TCTGGTGCTA GCACCCCTGG CCGCGGCGGG CGGAGGCAGC TCCCCCAGAC CCCGCTGACC
    CCCCGTCCCA GCATCACCTA TAAGACGGCC AACTCCTCAC CTGTCCACTT TGCCGGGGCT
    CAGACCAGCC TCCCTGCCTT TTCCCCCGGC CGGCTTAGCC GTGGCCTTTC GGAACACAAC
    GCCCTGCTCC AGAGAGACCC CCTCAGCCAG CCGCTGGCCC CCAGCTCTCG GATTGGCTCC
    GACCCTTACC TGGGGCAGCG TCTGGACAGT GAGGCTGCGG CCCGCACCCA GCTTGAGGAC
    ACGCTCACCT TCGAGGAGGC CGTGGCCACC AACTCGGGCC GCTCCTCCCG GACTTCCTAC
    GTGTCCTCCC TGACCTCGCA GTCCCACCCC CTCCGCCGGG TGCCCAACGG CTACCACTGC
    ACTCTGGGGC TCAGCTCGGG CGGGGGCGGC CGGGGGCGGC ACAGCTACCA CCACCCTGAC
    CAAGACCACT GGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220146
    Clone ID Related Accession (Same CDS sequence) XM_024998343.1
    Accession Version XM_024998343.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGCAGTGTT GAAGAGAGCA
    GCCACCAAGA AGAGCCGAAA CGACCTGATC CACGCGGAGG AGGGAGAGGA CCGCTTTGCA
    GACCTCTGTG CTGTGGGGTC CCCCTTCGCC CGAGCCAGCC TCAAGAGTGG GAAGACGGAG
    AGCTCGTCAT ACTTCCGCAG GAAGGAGAAG ATGTTCCGGT TCTTCATCCG GCGCCTGGTG
    AAGGCGCAGA GCTTCTACTG GGTGGTGCTG TGTGTGGTGG CCCTGAACAC GCTGTGCGTG
    GCCATGGTGC ACTACCAGCA GCCCCAGCGG CTCACCACGG CGCTGTACTT TGCAGAGTTT
    GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGG
    AGCTACTTCC GGTCCTCCTT CAACTGCTTC GATTTCGGGG TCATTGTGGG GAGCATTTTC
    GAAGTGGTCT GGGCGGCCAT CAAGCCAGGA ACCTCCTTCG GCATCAGTGT GCTGCGGGCG
    CTGCGGCTGC TGAGAATCTT CAAAGTCACC AAGTACTGGA GCTCCCTGCG GAACCTGGTG
    GTGTCCCTGC TCAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCCTGCT CTTCCTCTTC
    ATCGTGGTCT TCGCTCTGCT GGGCATGCAG CTCTTCGGGG GACAGTTCAA CTTCCAGGAT
    GAGACTCCGA CAACCAACTT TGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC
    CTGACCGGGG AGGACTGGAA TGCGGTGATG TACCATGGGA TCGAATCACA AGGTGGAGTC
    AGCAAGGGCA TGTTCTCGTC TGTGTACTTC ATTGTCCTGA CGCTGTTTGG GAACTACACC
    CTGCTGAACG TCTTCCTGGC CATCGCTGTG GACAACCTCG CCAATGCTCA GGAGCTGACC
    AAGGACGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA
    GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATTGCTGC CAGGCAGCAG
    AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTGCG GCTGCAGAAC
    CTGCGGGCCA GCTGTGAAGC GCTGTATAGC GAGATGGACC CCGAGGAGCG CCTGCGCTTC
    GCCACTTCCC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTTGTGGTG
    GAGCCAGGCC GGGATGGCGC GCGGGGGCCT GCGGGGGGCA AGGCCCGGCC CGAGGGCGGG
    GAGGCCGGCG AGGGTGCCGA CCCGCCGCGC AGGCACCACC GGCACCGCGA CAGGGACAGG
    GACAAGGCCG CAGCCCCGGC GGGGGAGCCA GACAGGGCGG ACGCCCCGAA GGCGGAGGGC
    GGGGAGCCGG GGGCCCGGGA GGAGCGGGCG CGACCGCGCC GCAGCCGCAG CAAGGAGGCC
    GCAGGCCCCC GCGAGGCCAG GAGCGAGCGA GGCCGCGGGC TGGGCCCGGA CGGCGGCCGG
    CGCCACCACC GGCGCGGCTC CCCGGAGGAG GCCGCGGAGC GCGAGCCCCG GCGCCACCGC
    GCGCACCGGC ACGCGCCGGA GCCGAGCAGG GAGGGCGCGC CCGGGAGCAA GGGCGAGCGG
    CGAGCGCGGC ACCGCGGCGG CTCCCGGGCC GGGCCCCGGG AGGCCGAGAG CGGCGAGGAG
    CCCTCCCGGC GGCACCGAGC GCGACACAAG GCGCCGCCCG CACACGAGGA GGCGGAGAAG
    GAGGCCGAGG CGGAGGACAG GGACAAGGAG CCCCGAAACC ACCAGCCCAG GGAGTCGCAC
    TGTGACCTGG AGGCCAGTGT GGTCATTGGT GTGGGCCCTG TGCATGCCCT GCCCAGCACT
    TGTCTAGAGA AGGTGGAAGA GCAGCCAGAG GATGCAGACA ATCAGCGGAA TGTGACTCGC
    ATGGGCAGTC AGCCCTCAGA TCTGAGCACC GCTGTGCGTA TCCCAGTGAC GCTGACCGGG
    CCTCCCGGGG AGACCACGGT TGTTCCCAGT GGTAACGTGG ACCTGGAAAG CCAAGCAGAA
    GGAAAGAAGG AGGTGGAAGC TGATGACGTG ATGAGGAGTG GCCCCCGACC CATTGTCCCG
    TACAGCTCCA TGTTCTGCTT GAGCCCCACC AACCTGCTCC GCCGCTTCTG CCACTACATT
    GTGACCATGC GGTACTTCGA GATGGTCATT CTCGTGGTCA TTGCCCTGAG TAGCATTGCC
    CTGGCTGCGG AGGATCCTGT GCAGACAGAC TCGCCCAGGA ACAACGTGCT GAAGTACATG
    GACTACATCT TCACAGGTGT CTTCACCTTT GAGATGGTGA TAAAGATGAT CGATTTGGGA
    CTGCTGCTGC ACCCCGGTGC CTACTTCCGG GATCTGTGGA ACATTCTGGA CTTCATTGTG
    GTCAGTGGGG CCCTGGTGGC GTTTGCCTTC TCAGGCTCCA AAGGGAAAGA CATCAGCACC
    ATCAAGTCCC TGAGGGTCCT GCGTGTGCTG AGGCCACTCA AGACCATCAA GCGGCTGCCC
    AAGCTCAAAC TCTTCAAGGG GAAGTTTTTC TACTGCACCG ACGAGTCCAA GGAGCTGGAG
    CGGGACTGCA GGGGCCAGTA TTTGGACTAC GAGAAGGAGG AGGTGGAAGC TCAGCCACGG
    CAGTGGAAGA AATACGACTT CCACTATGAC AACGTGCTGT GGGCCCTGCT AACGCTGTTC
    ACTGTGTCCA CGGGCGAAGG GTGGCCCATG GTGCTGAAAC ACTCGGTGGA TGCCACCTAC
    GAGGAGCAGG GCCCCAGCCC CGGGTTCCGC ATGGAGCTGT CCATCTTCTA TGTGGTCTAC
    TTCGTGGTCT TCCCCTTCTT CTTTGTGAAC ATCTTTGTGG CCTTGATCAT CATCACCTTC
    CAGGAGCAGG GGGACAAAGT CATGTCTGAG TGCAGCTTGG AGAAGAACGA GAGGGCTTGT
    ATCGACTTCG CCATTAGCGC CAAACCCCTG ACGCGGTACA TGCCCCAGAA CAAGCAGTCG
    TTCCAGTACA AGACGTGGAC GTTCGTGGTC TCACCACCCT TTGAGTATTT CATCATGGCC
    ATGATAGCCC TCAACACAGT GGTGCTGATG ATGAAGTTCT ACGATGCGCC CTACGAGTAC
    GAACTGATGT TGAAGTGTCT GAACATCGTG TTCACGTCCA TGTTCTCCAT GGAGTGTGTG
    CTCAAGGTCA TCGCCTTTGG AGTGCTGAAC TATTTCAGAG ACGCCTGGAA TGTCTTTGAC
    TTTGTCACTG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGAAACGAAC
    AACTTCATCA ACCTCAGCTT CCTCCGCCTC TTCCGTGCTG CCCGACTCAT CAAGCTGCTC
    CGCCAAGGCT ACACCATCCG CATTCTGCTC TGGACCTTCG TCCAGTCCTT CAAGGCCCTG
    CCCTATGTGT GCCTGCTCAT CGCCATGCTG TTCTTCATCT ACGCCATCAT CGGCATGCAG
    GTGTTTGGAA ACATTGCTCT GGATGACGAC ACCAGCATCA ACCGTCACAA TAACTTTCGG
    ACATTTCTTC AGGCCTTGAT GCTGCTCTTC AGGAGTGCCA CCGGGGAGGC CTGGCATGAG
    ATCATGCTGT CCTGCCTCAG CAGTCGGGCC TGTGATGAGC TCGCCAATGC CACGGAGTGT
    GGGAGTGACT TCGCCTACTT CTACTTTGTC TCCTTCATCT TCCTTTGCTC CTTTCTGATG
    TTGAACCTCT TTGTGGCTGT GATCATGGAC AATTTTGAGT ACCTTACGCG GGACTCTTCC
    ATCCTAGGGC CTCACCACCT GGACGAATTC ATCCGGGTCT GGGCCGAGTA CGACCCGGCT
    GCGTGTGGGC GCATCAGTTA CAATGACATG TTTGAGATGC TGAAACACAT GTCCCCGCCC
    CTGGGGCTGG GGAAGAAATG CCCTGCTCGT GTTGCGTACA AGCGCCTGGT TCGTATGAAC
    ATGCCCATCT CCAATGACGA CATGACCGTC CACTTCACAT CCACGCTGAT GGCCCTCATC
    CGGACTGCAT TGGAGATCAA GCTGGCTCCA GCTGGCACGA AGCAGCATCA GTGTGACGCA
    GAGCTGAGGA AGGAGATCTC CTCCGTGTGG GCCAATCTGC CCCAGAAGAC CCTGGACTTA
    CTGGTGCCAC CCCATAAACC CGACGAGATG ACGGTGGGGA AGGTGTACGC AGCTCTGATG
    ATATTCGACT TCTACAAACA GAACAAAACC ACCAGAGACC AGATTCACCA AGCTCCTGGA
    GGCCTGGCCC AGATGGGCCC CGTGTCCCTG TTCCACCCCC TGAAGGCCAC GCTGGAGCAG
    ACTCAGCCGG CTGTGCTCCG AGGAGCCCGC GTTTTCCTTC GGCAGAAGAG CTCGGCCTCC
    CTCAGCAACG GTGGGGCAGT ACAAACCCAA GAGGGCGGCA TCAAGGAGTC TGTCTCCTGG
    GGCACTCAGA GGACCCAGGA GGTCCCCTGC GAGGTGAGGA CACCCCTGGA GCGTGGCCAC
    TCCACAGAGA TCCCGGTGCA GACAGGAAAA CCGGCTGTGG ACGTCCAGAT GCAGAGCATG
    GTCCTGAGAG GCCCTGACGG GGAGCCCCAA CCTGGGCTGG AGAGCCAGGG CCGAGCAGCT
    TCCATGCCCC GCCTGGCAGC AGAGACTCAG CCACCCCCAG ATGCCAGCCC CATGAAGCGC
    TCCATCTCCA CACTGGCACC CCAGAGGCCC CATGTGGCTC ATCTGTGCAC CGCGGCCCTG
    GACCGCGCTC CAGCCAGCCA GGCCGCCCCC CCCCACCACC ACCGCTGCCA TCGCCGCAGG
    GACCGGAAGC AGAAGTCCCT GGAGAAGGGG CCCGGCCTGT CTGCAGACAC AGATGGCGCG
    CCCTACAGCA CCGCGGGGCC GGGCCCCCCG CCGCCCCCAG GGGATGGGGC AGCAGGCTGC
    CGGCGGGAGC GCAGGCAGGA GCGGGGCCGG TCCCAGGAGC GGAGGCAGCC CTCCTCCTCT
    TCCTCTGAGA AGCAGCGCTT CTACTCCTGT GACCGCTTTG GGGGCCGTGA GCCCCCGCAG
    CCCAAGCCCT CTCTCAGCAG CCATCCCACG TCGCCCACGG CCGGGCAGGA GCCAGGACCC
    CCGAGACAGG GTAGCGGCTC CGTGAACGGG AGCCCCCTGC TGTCGACATC TGGTGCTAGC
    ACCCCTGGCC GCGGCGGGCG GAGGCAGCTC CCCCAGACCC CGCTGACCCC CCGTCCCAGC
    ATCACCTATA AGACGGCCAA CTCCTCACCT GTCCACTTTG CCGGGGCTCA GACCAGCCTC
    CCTGCCTTTT CCCCCGGCCG GCTTAGCCGT GGCCTTTCGG AACACAACGC CCTGCTCCAG
    AGAGACCCCC TCAGCCAGCC GCTGGCCCCC AGCTCTCGGA TTGGCTCCGA CCCTTACCTG
    GGGCAGCGTC TGGACAGTGA GGCTGCGGCC CGCACCCAGC TTGAGGACAC GCTCACCTTC
    GAGGAGGCCG TGGCCACCAA CTCGGGCCGC TCCTCCCGGA CTTCCTACGT GTCCTCCCTG
    ACCTCGCAGT CCCACCCCCT CCGCCGGGTG CCCAACGGCT ACCACTGCAC TCTGGGGCTC
    AGCTCGGGCG GGGGCGGCCG GGGGCGGCAC AGCTACCACC ACCCTGACCA AGACCACTGG
    TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854111.1
    CDS140..7045
    Translation

    Target ORF information:

    RefSeq Version XM_024998343.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998343.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGCAGTGTT GAAGAGAGCA
    GCCACCAAGA AGAGCCGAAA CGACCTGATC CACGCGGAGG AGGGAGAGGA CCGCTTTGCA
    GACCTCTGTG CTGTGGGGTC CCCCTTCGCC CGAGCCAGCC TCAAGAGTGG GAAGACGGAG
    AGCTCGTCAT ACTTCCGCAG GAAGGAGAAG ATGTTCCGGT TCTTCATCCG GCGCCTGGTG
    AAGGCGCAGA GCTTCTACTG GGTGGTGCTG TGTGTGGTGG CCCTGAACAC GCTGTGCGTG
    GCCATGGTGC ACTACCAGCA GCCCCAGCGG CTCACCACGG CGCTGTACTT TGCAGAGTTT
    GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGG
    AGCTACTTCC GGTCCTCCTT CAACTGCTTC GATTTCGGGG TCATTGTGGG GAGCATTTTC
    GAAGTGGTCT GGGCGGCCAT CAAGCCAGGA ACCTCCTTCG GCATCAGTGT GCTGCGGGCG
    CTGCGGCTGC TGAGAATCTT CAAAGTCACC AAGTACTGGA GCTCCCTGCG GAACCTGGTG
    GTGTCCCTGC TCAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCCTGCT CTTCCTCTTC
    ATCGTGGTCT TCGCTCTGCT GGGCATGCAG CTCTTCGGGG GACAGTTCAA CTTCCAGGAT
    GAGACTCCGA CAACCAACTT TGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC
    CTGACCGGGG AGGACTGGAA TGCGGTGATG TACCATGGGA TCGAATCACA AGGTGGAGTC
    AGCAAGGGCA TGTTCTCGTC TGTGTACTTC ATTGTCCTGA CGCTGTTTGG GAACTACACC
    CTGCTGAACG TCTTCCTGGC CATCGCTGTG GACAACCTCG CCAATGCTCA GGAGCTGACC
    AAGGACGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA
    GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATTGCTGC CAGGCAGCAG
    AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTGCG GCTGCAGAAC
    CTGCGGGCCA GCTGTGAAGC GCTGTATAGC GAGATGGACC CCGAGGAGCG CCTGCGCTTC
    GCCACTTCCC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTTGTGGTG
    GAGCCAGGCC GGGATGGCGC GCGGGGGCCT GCGGGGGGCA AGGCCCGGCC CGAGGGCGGG
    GAGGCCGGCG AGGGTGCCGA CCCGCCGCGC AGGCACCACC GGCACCGCGA CAGGGACAGG
    GACAAGGCCG CAGCCCCGGC GGGGGAGCCA GACAGGGCGG ACGCCCCGAA GGCGGAGGGC
    GGGGAGCCGG GGGCCCGGGA GGAGCGGGCG CGACCGCGCC GCAGCCGCAG CAAGGAGGCC
    GCAGGCCCCC GCGAGGCCAG GAGCGAGCGA GGCCGCGGGC TGGGCCCGGA CGGCGGCCGG
    CGCCACCACC GGCGCGGCTC CCCGGAGGAG GCCGCGGAGC GCGAGCCCCG GCGCCACCGC
    GCGCACCGGC ACGCGCCGGA GCCGAGCAGG GAGGGCGCGC CCGGGAGCAA GGGCGAGCGG
    CGAGCGCGGC ACCGCGGCGG CTCCCGGGCC GGGCCCCGGG AGGCCGAGAG CGGCGAGGAG
    CCCTCCCGGC GGCACCGAGC GCGACACAAG GCGCCGCCCG CACACGAGGA GGCGGAGAAG
    GAGGCCGAGG CGGAGGACAG GGACAAGGAG CCCCGAAACC ACCAGCCCAG GGAGTCGCAC
    TGTGACCTGG AGGCCAGTGT GGTCATTGGT GTGGGCCCTG TGCATGCCCT GCCCAGCACT
    TGTCTAGAGA AGGTGGAAGA GCAGCCAGAG GATGCAGACA ATCAGCGGAA TGTGACTCGC
    ATGGGCAGTC AGCCCTCAGA TCTGAGCACC GCTGTGCGTA TCCCAGTGAC GCTGACCGGG
    CCTCCCGGGG AGACCACGGT TGTTCCCAGT GGTAACGTGG ACCTGGAAAG CCAAGCAGAA
    GGAAAGAAGG AGGTGGAAGC TGATGACGTG ATGAGGAGTG GCCCCCGACC CATTGTCCCG
    TACAGCTCCA TGTTCTGCTT GAGCCCCACC AACCTGCTCC GCCGCTTCTG CCACTACATT
    GTGACCATGC GGTACTTCGA GATGGTCATT CTCGTGGTCA TTGCCCTGAG TAGCATTGCC
    CTGGCTGCGG AGGATCCTGT GCAGACAGAC TCGCCCAGGA ACAACGTGCT GAAGTACATG
    GACTACATCT TCACAGGTGT CTTCACCTTT GAGATGGTGA TAAAGATGAT CGATTTGGGA
    CTGCTGCTGC ACCCCGGTGC CTACTTCCGG GATCTGTGGA ACATTCTGGA CTTCATTGTG
    GTCAGTGGGG CCCTGGTGGC GTTTGCCTTC TCAGGCTCCA AAGGGAAAGA CATCAGCACC
    ATCAAGTCCC TGAGGGTCCT GCGTGTGCTG AGGCCACTCA AGACCATCAA GCGGCTGCCC
    AAGCTCAAAC TCTTCAAGGG GAAGTTTTTC TACTGCACCG ACGAGTCCAA GGAGCTGGAG
    CGGGACTGCA GGGGCCAGTA TTTGGACTAC GAGAAGGAGG AGGTGGAAGC TCAGCCACGG
    CAGTGGAAGA AATACGACTT CCACTATGAC AACGTGCTGT GGGCCCTGCT AACGCTGTTC
    ACTGTGTCCA CGGGCGAAGG GTGGCCCATG GTGCTGAAAC ACTCGGTGGA TGCCACCTAC
    GAGGAGCAGG GCCCCAGCCC CGGGTTCCGC ATGGAGCTGT CCATCTTCTA TGTGGTCTAC
    TTCGTGGTCT TCCCCTTCTT CTTTGTGAAC ATCTTTGTGG CCTTGATCAT CATCACCTTC
    CAGGAGCAGG GGGACAAAGT CATGTCTGAG TGCAGCTTGG AGAAGAACGA GAGGGCTTGT
    ATCGACTTCG CCATTAGCGC CAAACCCCTG ACGCGGTACA TGCCCCAGAA CAAGCAGTCG
    TTCCAGTACA AGACGTGGAC GTTCGTGGTC TCACCACCCT TTGAGTATTT CATCATGGCC
    ATGATAGCCC TCAACACAGT GGTGCTGATG ATGAAGTTCT ACGATGCGCC CTACGAGTAC
    GAACTGATGT TGAAGTGTCT GAACATCGTG TTCACGTCCA TGTTCTCCAT GGAGTGTGTG
    CTCAAGGTCA TCGCCTTTGG AGTGCTGAAC TATTTCAGAG ACGCCTGGAA TGTCTTTGAC
    TTTGTCACTG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGAAACGAAC
    AACTTCATCA ACCTCAGCTT CCTCCGCCTC TTCCGTGCTG CCCGACTCAT CAAGCTGCTC
    CGCCAAGGCT ACACCATCCG CATTCTGCTC TGGACCTTCG TCCAGTCCTT CAAGGCCCTG
    CCCTATGTGT GCCTGCTCAT CGCCATGCTG TTCTTCATCT ACGCCATCAT CGGCATGCAG
    GTGTTTGGAA ACATTGCTCT GGATGACGAC ACCAGCATCA ACCGTCACAA TAACTTTCGG
    ACATTTCTTC AGGCCTTGAT GCTGCTCTTC AGGAGTGCCA CCGGGGAGGC CTGGCATGAG
    ATCATGCTGT CCTGCCTCAG CAGTCGGGCC TGTGATGAGC TCGCCAATGC CACGGAGTGT
    GGGAGTGACT TCGCCTACTT CTACTTTGTC TCCTTCATCT TCCTTTGCTC CTTTCTGATG
    TTGAACCTCT TTGTGGCTGT GATCATGGAC AATTTTGAGT ACCTTACGCG GGACTCTTCC
    ATCCTAGGGC CTCACCACCT GGACGAATTC ATCCGGGTCT GGGCCGAGTA CGACCCGGCT
    GCGTGTGGGC GCATCAGTTA CAATGACATG TTTGAGATGC TGAAACACAT GTCCCCGCCC
    CTGGGGCTGG GGAAGAAATG CCCTGCTCGT GTTGCGTACA AGCGCCTGGT TCGTATGAAC
    ATGCCCATCT CCAATGACGA CATGACCGTC CACTTCACAT CCACGCTGAT GGCCCTCATC
    CGGACTGCAT TGGAGATCAA GCTGGCTCCA GCTGGCACGA AGCAGCATCA GTGTGACGCA
    GAGCTGAGGA AGGAGATCTC CTCCGTGTGG GCCAATCTGC CCCAGAAGAC CCTGGACTTA
    CTGGTGCCAC CCCATAAACC CGACGAGATG ACGGTGGGGA AGGTGTACGC AGCTCTGATG
    ATATTCGACT TCTACAAACA GAACAAAACC ACCAGAGACC AGATTCACCA AGCTCCTGGA
    GGCCTGGCCC AGATGGGCCC CGTGTCCCTG TTCCACCCCC TGAAGGCCAC GCTGGAGCAG
    ACTCAGCCGG CTGTGCTCCG AGGAGCCCGC GTTTTCCTTC GGCAGAAGAG CTCGGCCTCC
    CTCAGCAACG GTGGGGCAGT ACAAACCCAA GAGGGCGGCA TCAAGGAGTC TGTCTCCTGG
    GGCACTCAGA GGACCCAGGA GGTCCCCTGC GAGGTGAGGA CACCCCTGGA GCGTGGCCAC
    TCCACAGAGA TCCCGGTGCA GACAGGAAAA CCGGCTGTGG ACGTCCAGAT GCAGAGCATG
    GTCCTGAGAG GCCCTGACGG GGAGCCCCAA CCTGGGCTGG AGAGCCAGGG CCGAGCAGCT
    TCCATGCCCC GCCTGGCAGC AGAGACTCAG CCACCCCCAG ATGCCAGCCC CATGAAGCGC
    TCCATCTCCA CACTGGCACC CCAGAGGCCC CATGTGGCTC ATCTGTGCAC CGCGGCCCTG
    GACCGCGCTC CAGCCAGCCA GGCCGCCCCC CCCCACCACC ACCGCTGCCA TCGCCGCAGG
    GACCGGAAGC AGAAGTCCCT GGAGAAGGGG CCCGGCCTGT CTGCAGACAC AGATGGCGCG
    CCCTACAGCA CCGCGGGGCC GGGCCCCCCG CCGCCCCCAG GGGATGGGGC AGCAGGCTGC
    CGGCGGGAGC GCAGGCAGGA GCGGGGCCGG TCCCAGGAGC GGAGGCAGCC CTCCTCCTCT
    TCCTCTGAGA AGCAGCGCTT CTACTCCTGT GACCGCTTTG GGGGCCGTGA GCCCCCGCAG
    CCCAAGCCCT CTCTCAGCAG CCATCCCACG TCGCCCACGG CCGGGCAGGA GCCAGGACCC
    CCGAGACAGG GTAGCGGCTC CGTGAACGGG AGCCCCCTGC TGTCGACATC TGGTGCTAGC
    ACCCCTGGCC GCGGCGGGCG GAGGCAGCTC CCCCAGACCC CGCTGACCCC CCGTCCCAGC
    ATCACCTATA AGACGGCCAA CTCCTCACCT GTCCACTTTG CCGGGGCTCA GACCAGCCTC
    CCTGCCTTTT CCCCCGGCCG GCTTAGCCGT GGCCTTTCGG AACACAACGC CCTGCTCCAG
    AGAGACCCCC TCAGCCAGCC GCTGGCCCCC AGCTCTCGGA TTGGCTCCGA CCCTTACCTG
    GGGCAGCGTC TGGACAGTGA GGCTGCGGCC CGCACCCAGC TTGAGGACAC GCTCACCTTC
    GAGGAGGCCG TGGCCACCAA CTCGGGCCGC TCCTCCCGGA CTTCCTACGT GTCCTCCCTG
    ACCTCGCAGT CCCACCCCCT CCGCCGGGTG CCCAACGGCT ACCACTGCAC TCTGGGGCTC
    AGCTCGGGCG GGGGCGGCCG GGGGCGGCAC AGCTACCACC ACCCTGACCA AGACCACTGG
    TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220148
    Clone ID Related Accession (Same CDS sequence) XM_024998345.1
    Accession Version XM_024998345.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC
    ACCAAGAAGA GCCGAAACGA CCTGATCCAC GCGGAGGAGG GAGAGGACCG CTTTGCAGAC
    CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGCCTCA AGAGTGGGAA GACGGAGAGC
    TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG
    GCGCAGAGCT TCTACTGGGT GGTGCTGTGT GTGGTGGCCC TGAACACGCT GTGCGTGGCC
    ATGGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCGC TGTACTTTGC AGAGTTTGTT
    TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGGAGC
    TACTTCCGGT CCTCCTTCAA CTGCTTCGAT TTCGGGGTCA TTGTGGGGAG CATTTTCGAA
    GTGGTCTGGG CGGCCATCAA GCCAGGAACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG
    CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG
    TCCCTGCTCA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC
    GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT CCAGGATGAG
    ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG
    ACCGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AATCACAAGG TGGAGTCAGC
    AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG
    CTGAACGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG
    GACGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA
    GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCTGCCAG GCAGCAGAAC
    TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG
    CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGCCT GCGCTTCGCC
    ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG
    CCAGGCCGGG ATGGCGCGCG GGGGCCTGCG GGGGGCAAGG CCCGGCCCGA GGGCGGGGAG
    GCCGGCGAGG GTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACAGGGAC
    AAGGCCGCAG CCCCGGCGGG GGAGCCAGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG
    GAGCCGGGGG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA
    GGCCCCCGCG AGGCCAGGAG CGAGCGAGGC CGCGGGCTGG GCCCGGACGG CGGCCGGCGC
    CACCACCGGC GCGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG
    CACCGGCACG CGCCGGAGCC GAGCAGGGAG GGCGCGCCCG GGAGCAAGGG CGAGCGGCGA
    GCGCGGCACC GCGGCGGCTC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCC
    TCCCGGCGGC ACCGAGCGCG ACACAAGGCG CCGCCCGCAC ACGAGGAGGC GGAGAAGGAG
    GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGAAACCACC AGCCCAGGGA GTCGCACTGT
    GACCTGGAGG CCAGTGTGGT CATTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACTTGT
    CTAGAGAAGG TGGAAGAGCA GCCAGAGGAT GCAGACAATC AGCGGAATGT GACTCGCATG
    GGCAGTCAGC CCTCAGATCT GAGCACCGCT GTGCGTATCC CAGTGACGCT GACCGGGCCT
    CCCGGGGAGA CCACGGTTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA
    AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGTGGCC CCCGACCCAT TGTCCCGTAC
    AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTCCGCC GCTTCTGCCA CTACATTGTG
    ACCATGCGGT ACTTCGAGAT GGTCATTCTC GTGGTCATTG CCCTGAGTAG CATTGCCCTG
    GCTGCGGAGG ATCCTGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC
    TACATCTTCA CAGGTGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA TTTGGGACTG
    CTGCTGCACC CCGGTGCCTA CTTCCGGGAT CTGTGGAACA TTCTGGACTT CATTGTGGTC
    AGTGGGGCCC TGGTGGCGTT TGCCTTCTCA GGCTCCAAAG GGAAAGACAT CAGCACCATC
    AAGTCCCTGA GGGTCCTGCG TGTGCTGAGG CCACTCAAGA CCATCAAGCG GCTGCCCAAG
    CTCAAAGCTG TGTTTGACTG TGTGGTGAAC TCCCTGAAGA ACGTCCTCAA CATCCTGATC
    GTCTACATGC TCTTCATGTT CATATTTGCT GTCATCGCGG TACAGCTCTT CAAGGGGAAG
    TTTTTCTACT GCACCGACGA GTCCAAGGAG CTGGAGCGGG ACTGCAGGGG CCAGTATTTG
    GACTACGAGA AGGAGGAGGT GGAAGCTCAG CCACGGCAGT GGAAGAAATA CGACTTCCAC
    TATGACAACG TGCTGTGGGC CCTGCTAACG CTGTTCACTG TGTCCACGGG CGAAGGGTGG
    CCCATGGTGC TGAAACACTC GGTGGATGCC ACCTACGAGG AGCAGGGCCC CAGCCCCGGG
    TTCCGCATGG AGCTGTCCAT CTTCTATGTG GTCTACTTCG TGGTCTTCCC CTTCTTCTTT
    GTGAACATCT TTGTGGCCTT GATCATCATC ACCTTCCAGG AGCAGGGGGA CAAAGTCATG
    TCTGAGTGCA GCTTGGAGAA GAACGAGAGG GCTTGTATCG ACTTCGCCAT TAGCGCCAAA
    CCCCTGACGC GGTACATGCC CCAGAACAAG CAGTCGTTCC AGTACAAGAC GTGGACGTTC
    GTGGTCTCAC CACCCTTTGA GTATTTCATC ATGGCCATGA TAGCCCTCAA CACAGTGGTG
    CTGATGATGA AGTTCTACGA TGCGCCCTAC GAGTACGAAC TGATGTTGAA GTGTCTGAAC
    ATCGTGTTCA CGTCCATGTT CTCCATGGAG TGTGTGCTCA AGGTCATCGC CTTTGGAGTG
    CTGAACTATT TCAGAGACGC CTGGAATGTC TTTGACTTTG TCACTGTGTT GGGAAGTATT
    ACTGATATTT TAGTAACAGA GATTGCGGAA ACGAACAACT TCATCAACCT CAGCTTCCTC
    CGCCTCTTCC GTGCTGCCCG ACTCATCAAG CTGCTCCGCC AAGGCTACAC CATCCGCATT
    CTGCTCTGGA CCTTCGTCCA GTCCTTCAAG GCCCTGCCCT ATGTGTGCCT GCTCATCGCC
    ATGCTGTTCT TCATCTACGC CATCATCGGC ATGCAGGTGT TTGGAAACAT TGCTCTGGAT
    GACGACACCA GCATCAACCG TCACAATAAC TTTCGGACAT TTCTTCAGGC CTTGATGCTG
    CTCTTCAGGA GTGCCACCGG GGAGGCCTGG CATGAGATCA TGCTGTCCTG CCTCAGCAGT
    CGGGCCTGTG ATGAGCTCGC CAATGCCACG GAGTGTGGGA GTGACTTCGC CTACTTCTAC
    TTTGTCTCCT TCATCTTCCT TTGCTCCTTT CTGATGTTGA ACCTCTTTGT GGCTGTGATC
    ATGGACAATT TTGAGTACCT TACGCGGGAC TCTTCCATCC TAGGGCCTCA CCACCTGGAC
    GAATTCATCC GGGTCTGGGC CGAGTACGAC CCGGCTGCGT GTGGGCGCAT CAGTTACAAT
    GACATGTTTG AGATGCTGAA ACACATGTCC CCGCCCCTGG GGCTGGGGAA GAAATGCCCT
    GCTCGTGTTG CGTACAAGCG CCTGGTTCGT ATGAACATGC CCATCTCCAA TGACGACATG
    ACCGTCCACT TCACATCCAC GCTGATGGCC CTCATCCGGA CTGCATTGGA GATCAAGCTG
    GCTCCAGCTG GCACGAAGCA GCATCAGTGT GACGCAGAGC TGAGGAAGGA GATCTCCTCC
    GTGTGGGCCA ATCTGCCCCA GAAGACCCTG GACTTACTGG TGCCACCCCA TAAACCCGAC
    GAGATGACGG TGGGGAAGGT GTACGCAGCT CTGATGATAT TCGACTTCTA CAAACAGAAC
    AAAACCACCA GAGACCAGAT TCACCAAGCT CCTGGAGGCC TGGCCCAGAT GGGCCCCGTG
    TCCCTGTTCC ACCCCCTGAA GGCCACGCTG GAGCAGACTC AGCCGGCTGT GCTCCGAGGA
    GCCCGCGTTT TCCTTCGGCA GAAGAGCTCG GCCTCCCTCA GCAACGGTGG GGCAGTACAA
    ACCCAAGAGG GCGGCATCAA GGAGTCTGTC TCCTGGGGCA CTCAGAGGAC CCAGGAGGTC
    CCCTGCGAGG TGAGGACACC CCTGGAGCGT GGCCACTCCA CAGAGATCCC GGTGCAGACA
    GGAAAACCGG CTGTGGACGT CCAGATGCAG AGCATGGTCC TGAGAGGCCC TGACGGGGAG
    CCCCAACCTG GGCTGGAGAG CCAGGGCCGA GCAGCTTCCA TGCCCCGCCT GGCAGCAGAG
    ACTCAGCCAC CCCCAGATGC CAGCCCCATG AAGCGCTCCA TCTCCACACT GGCACCCCAG
    AGGCCCCATG TGGCTCATCT GTGCACCGCG GCCCTGGACC GCGCTCCAGC CAGCCAGGCC
    GCCCCCCCCC ACCACCACCG CTGCCATCGC CGCAGGGACC GGAAGCAGAA GTCCCTGGAG
    AAGGGGCCCG GCCTGTCTGC AGACACAGAT GGCGCGCCCT ACAGCACCGC GGGGCCGGGC
    CCCCCGCCGC CCCCAGGGGA TGGGGCAGCA GGCTGCCGGC GGGAGCGCAG GCAGGAGCGG
    GGCCGGTCCC AGGAGCGGAG GCAGCCCTCC TCCTCTTCCT CTGAGAAGCA GCGCTTCTAC
    TCCTGTGACC GCTTTGGGGG CCGTGAGCCC CCGCAGCCCA AGCCCTCTCT CAGCAGCCAT
    CCCACGTCGC CCACGGCCGG GCAGGAGCCA GGGCCTTTTC CCCCGGCCGG CTTAGCCGTG
    GCCTTTCGGA ACACAACGCC CTGCTCCAGA GAGACCCCCT CAGCCAGCCG CTGGCCCCCA
    GCTCTCGGAT TGGCTCCGAC CCTTACCTGG GGCAGCGTCT GGACAGTGAG GCTGCGGCCC
    GCACCCAGCT TGAGGACACG CTCACCTTCG AGGAGGCCGT GGCCACCAAC TCGGGCCGCT
    CCTCCCGGAC TTCCTACGTG TCCTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854113.1
    CDS141..6830
    Translation

    Target ORF information:

    RefSeq Version XM_024998345.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998345.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC
    ACCAAGAAGA GCCGAAACGA CCTGATCCAC GCGGAGGAGG GAGAGGACCG CTTTGCAGAC
    CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGCCTCA AGAGTGGGAA GACGGAGAGC
    TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG
    GCGCAGAGCT TCTACTGGGT GGTGCTGTGT GTGGTGGCCC TGAACACGCT GTGCGTGGCC
    ATGGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCGC TGTACTTTGC AGAGTTTGTT
    TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGGAGC
    TACTTCCGGT CCTCCTTCAA CTGCTTCGAT TTCGGGGTCA TTGTGGGGAG CATTTTCGAA
    GTGGTCTGGG CGGCCATCAA GCCAGGAACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG
    CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG
    TCCCTGCTCA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC
    GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT CCAGGATGAG
    ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG
    ACCGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AATCACAAGG TGGAGTCAGC
    AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG
    CTGAACGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG
    GACGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA
    GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCTGCCAG GCAGCAGAAC
    TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG
    CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGCCT GCGCTTCGCC
    ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG
    CCAGGCCGGG ATGGCGCGCG GGGGCCTGCG GGGGGCAAGG CCCGGCCCGA GGGCGGGGAG
    GCCGGCGAGG GTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACAGGGAC
    AAGGCCGCAG CCCCGGCGGG GGAGCCAGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG
    GAGCCGGGGG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA
    GGCCCCCGCG AGGCCAGGAG CGAGCGAGGC CGCGGGCTGG GCCCGGACGG CGGCCGGCGC
    CACCACCGGC GCGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG
    CACCGGCACG CGCCGGAGCC GAGCAGGGAG GGCGCGCCCG GGAGCAAGGG CGAGCGGCGA
    GCGCGGCACC GCGGCGGCTC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCC
    TCCCGGCGGC ACCGAGCGCG ACACAAGGCG CCGCCCGCAC ACGAGGAGGC GGAGAAGGAG
    GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGAAACCACC AGCCCAGGGA GTCGCACTGT
    GACCTGGAGG CCAGTGTGGT CATTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACTTGT
    CTAGAGAAGG TGGAAGAGCA GCCAGAGGAT GCAGACAATC AGCGGAATGT GACTCGCATG
    GGCAGTCAGC CCTCAGATCT GAGCACCGCT GTGCGTATCC CAGTGACGCT GACCGGGCCT
    CCCGGGGAGA CCACGGTTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA
    AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGTGGCC CCCGACCCAT TGTCCCGTAC
    AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTCCGCC GCTTCTGCCA CTACATTGTG
    ACCATGCGGT ACTTCGAGAT GGTCATTCTC GTGGTCATTG CCCTGAGTAG CATTGCCCTG
    GCTGCGGAGG ATCCTGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC
    TACATCTTCA CAGGTGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA TTTGGGACTG
    CTGCTGCACC CCGGTGCCTA CTTCCGGGAT CTGTGGAACA TTCTGGACTT CATTGTGGTC
    AGTGGGGCCC TGGTGGCGTT TGCCTTCTCA GGCTCCAAAG GGAAAGACAT CAGCACCATC
    AAGTCCCTGA GGGTCCTGCG TGTGCTGAGG CCACTCAAGA CCATCAAGCG GCTGCCCAAG
    CTCAAAGCTG TGTTTGACTG TGTGGTGAAC TCCCTGAAGA ACGTCCTCAA CATCCTGATC
    GTCTACATGC TCTTCATGTT CATATTTGCT GTCATCGCGG TACAGCTCTT CAAGGGGAAG
    TTTTTCTACT GCACCGACGA GTCCAAGGAG CTGGAGCGGG ACTGCAGGGG CCAGTATTTG
    GACTACGAGA AGGAGGAGGT GGAAGCTCAG CCACGGCAGT GGAAGAAATA CGACTTCCAC
    TATGACAACG TGCTGTGGGC CCTGCTAACG CTGTTCACTG TGTCCACGGG CGAAGGGTGG
    CCCATGGTGC TGAAACACTC GGTGGATGCC ACCTACGAGG AGCAGGGCCC CAGCCCCGGG
    TTCCGCATGG AGCTGTCCAT CTTCTATGTG GTCTACTTCG TGGTCTTCCC CTTCTTCTTT
    GTGAACATCT TTGTGGCCTT GATCATCATC ACCTTCCAGG AGCAGGGGGA CAAAGTCATG
    TCTGAGTGCA GCTTGGAGAA GAACGAGAGG GCTTGTATCG ACTTCGCCAT TAGCGCCAAA
    CCCCTGACGC GGTACATGCC CCAGAACAAG CAGTCGTTCC AGTACAAGAC GTGGACGTTC
    GTGGTCTCAC CACCCTTTGA GTATTTCATC ATGGCCATGA TAGCCCTCAA CACAGTGGTG
    CTGATGATGA AGTTCTACGA TGCGCCCTAC GAGTACGAAC TGATGTTGAA GTGTCTGAAC
    ATCGTGTTCA CGTCCATGTT CTCCATGGAG TGTGTGCTCA AGGTCATCGC CTTTGGAGTG
    CTGAACTATT TCAGAGACGC CTGGAATGTC TTTGACTTTG TCACTGTGTT GGGAAGTATT
    ACTGATATTT TAGTAACAGA GATTGCGGAA ACGAACAACT TCATCAACCT CAGCTTCCTC
    CGCCTCTTCC GTGCTGCCCG ACTCATCAAG CTGCTCCGCC AAGGCTACAC CATCCGCATT
    CTGCTCTGGA CCTTCGTCCA GTCCTTCAAG GCCCTGCCCT ATGTGTGCCT GCTCATCGCC
    ATGCTGTTCT TCATCTACGC CATCATCGGC ATGCAGGTGT TTGGAAACAT TGCTCTGGAT
    GACGACACCA GCATCAACCG TCACAATAAC TTTCGGACAT TTCTTCAGGC CTTGATGCTG
    CTCTTCAGGA GTGCCACCGG GGAGGCCTGG CATGAGATCA TGCTGTCCTG CCTCAGCAGT
    CGGGCCTGTG ATGAGCTCGC CAATGCCACG GAGTGTGGGA GTGACTTCGC CTACTTCTAC
    TTTGTCTCCT TCATCTTCCT TTGCTCCTTT CTGATGTTGA ACCTCTTTGT GGCTGTGATC
    ATGGACAATT TTGAGTACCT TACGCGGGAC TCTTCCATCC TAGGGCCTCA CCACCTGGAC
    GAATTCATCC GGGTCTGGGC CGAGTACGAC CCGGCTGCGT GTGGGCGCAT CAGTTACAAT
    GACATGTTTG AGATGCTGAA ACACATGTCC CCGCCCCTGG GGCTGGGGAA GAAATGCCCT
    GCTCGTGTTG CGTACAAGCG CCTGGTTCGT ATGAACATGC CCATCTCCAA TGACGACATG
    ACCGTCCACT TCACATCCAC GCTGATGGCC CTCATCCGGA CTGCATTGGA GATCAAGCTG
    GCTCCAGCTG GCACGAAGCA GCATCAGTGT GACGCAGAGC TGAGGAAGGA GATCTCCTCC
    GTGTGGGCCA ATCTGCCCCA GAAGACCCTG GACTTACTGG TGCCACCCCA TAAACCCGAC
    GAGATGACGG TGGGGAAGGT GTACGCAGCT CTGATGATAT TCGACTTCTA CAAACAGAAC
    AAAACCACCA GAGACCAGAT TCACCAAGCT CCTGGAGGCC TGGCCCAGAT GGGCCCCGTG
    TCCCTGTTCC ACCCCCTGAA GGCCACGCTG GAGCAGACTC AGCCGGCTGT GCTCCGAGGA
    GCCCGCGTTT TCCTTCGGCA GAAGAGCTCG GCCTCCCTCA GCAACGGTGG GGCAGTACAA
    ACCCAAGAGG GCGGCATCAA GGAGTCTGTC TCCTGGGGCA CTCAGAGGAC CCAGGAGGTC
    CCCTGCGAGG TGAGGACACC CCTGGAGCGT GGCCACTCCA CAGAGATCCC GGTGCAGACA
    GGAAAACCGG CTGTGGACGT CCAGATGCAG AGCATGGTCC TGAGAGGCCC TGACGGGGAG
    CCCCAACCTG GGCTGGAGAG CCAGGGCCGA GCAGCTTCCA TGCCCCGCCT GGCAGCAGAG
    ACTCAGCCAC CCCCAGATGC CAGCCCCATG AAGCGCTCCA TCTCCACACT GGCACCCCAG
    AGGCCCCATG TGGCTCATCT GTGCACCGCG GCCCTGGACC GCGCTCCAGC CAGCCAGGCC
    GCCCCCCCCC ACCACCACCG CTGCCATCGC CGCAGGGACC GGAAGCAGAA GTCCCTGGAG
    AAGGGGCCCG GCCTGTCTGC AGACACAGAT GGCGCGCCCT ACAGCACCGC GGGGCCGGGC
    CCCCCGCCGC CCCCAGGGGA TGGGGCAGCA GGCTGCCGGC GGGAGCGCAG GCAGGAGCGG
    GGCCGGTCCC AGGAGCGGAG GCAGCCCTCC TCCTCTTCCT CTGAGAAGCA GCGCTTCTAC
    TCCTGTGACC GCTTTGGGGG CCGTGAGCCC CCGCAGCCCA AGCCCTCTCT CAGCAGCCAT
    CCCACGTCGC CCACGGCCGG GCAGGAGCCA GGGCCTTTTC CCCCGGCCGG CTTAGCCGTG
    GCCTTTCGGA ACACAACGCC CTGCTCCAGA GAGACCCCCT CAGCCAGCCG CTGGCCCCCA
    GCTCTCGGAT TGGCTCCGAC CCTTACCTGG GGCAGCGTCT GGACAGTGAG GCTGCGGCCC
    GCACCCAGCT TGAGGACACG CTCACCTTCG AGGAGGCCGT GGCCACCAAC TCGGGCCGCT
    CCTCCCGGAC TTCCTACGTG TCCTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36127
    Clone ID Related Accession (Same CDS sequence) NM_174632.2
    Accession Version NM_174632.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product voltage-dependent N-type calcium channel subunit alpha-1B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF173882.1. On or before Mar 29, 2005 this sequence version replaced XM_598694.1, NM_174632.1. ##Evidence-Data-START## Transcript exon combination :: AF173882.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145437, SAMN03145443 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC
    ACCAAGAAGA GCCGAAACGA CCTGATCCAC GCGGAGGAGG GAGAGGACCG CTTTGCAGAC
    CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGCCTCA AGAGTGGGAA GACGGAGAGC
    TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG
    GCGCAGAGCT TCTACTGGGT GGTGCTGTGT GTGGTGGCCC TGAACACGCT GTGCGTGGCC
    ATGGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCGC TGTACTTTGC AGAGTTTGTT
    TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGGAGC
    TACTTCCGGT CCTCCTTCAA CTGCTTCGAT TTCGGGGTCA TTGTGGGGAG CATTTTCGAA
    GTGGTCTGGG CGGCCATCAA GCCAGGAACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG
    CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG
    TCCCTGCTCA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC
    GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT CCAGGATGAG
    ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG
    ACCGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AATCACAAGG TGGAGTCAGC
    AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG
    CTGAACGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG
    GACGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA
    GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCTGCCAG GCAGCAGAAC
    TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG
    CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGCCT GCGCTTCGCC
    ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG
    CCAGGCCGGG ATGGCGCGCG GGGGCCTGCG GGGGGCAAGG CCCGGCCCGA GGGCGGAGAG
    GCCGGCGAGG GTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACAGGGAC
    AAGGCCGCAG CCCCGGCGGG GGAGCCAGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG
    GAGCCGGGGG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA
    GGCCCCCGCG AGGCCAGGAG CGAGCGAGGC CGCGGGCTGG GCCCGGACGG CGGCCGGCGC
    CACCACCGGC GCGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG
    CACCGGCACG CGCCGGAGCC GAGCAGGGAG GGCGCGCCCG GGAGCAAGGG CGAGCGGCGA
    GCGCGGCACC GCGGCGGCTC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCC
    TCCCGGCGGC ACCGAGCGCG ACACAAGGCG CCGCCCGCAC ACGAGGAGGC GGAGAAGGAG
    GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGAAACCACC AGCCCAGGGA GTCGCACTGT
    GACCTGGAGG CCAGTGTGGT CATTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACTTGT
    CTAGAGAAGG TGGAAGAGCA GCCAGAGGAT GCAGACAATC AGCGGAATGT GACTCGCATG
    GGCAGTCAGC CCTCAGATCT GAGCACCGCT GTGCGTATCC CAGTGACGCT GACCGGGCCT
    CCCGGGGAGA CCACGGTTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA
    AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGTGGCC CCCGACCCAT TGTCCCGTAC
    AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTCCGCC GCTTCTGCCA CTACATTGTG
    ACCATGCAGT ACTTCGAGAT GGTCATTCTC GTGGTCATTG CCCTGAGTAG CATTGCCCTG
    GCTGCGGAGG ATCCTGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC
    TACATCTTCA CAGGTGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA TTTGGGACTG
    CTGCTGCACC CCGGTGCCTA CTTCCGGGAT CTGTGGAACA TTCTGGACTT CATTGTGGTC
    AGTGGGGCCC TGGTGGCGTT TGCCTTCTCA GGCTCCAAAG GGAAAGACAT CAGCACCATC
    AAGTCCCTGA GGGTCCTGCG TGTGCTGAGG CCACTCAAGA CCATCAAGCG GCTGCCCAAG
    CTCAAAGCTG TGTTTGACTG TGTGGTGAAC TCCCTGAAGA ACGTCCTCAA CATCCTGATC
    GTCTACATGC TCTTCATGTT CATATTTGCT GTCATCGCGG TACAGCTCTT CAAGGGGAAG
    TTTTTCTACT GCACCGACGA GTCCAAGGAG CTGGAGCGGG ACTGCAGGGG CCAGTATTTG
    GACTACGAGA AGGAGGAGGT GGAAGCTCAG CCGCGGCAGT GGAAGAAATA CGACTTCCAC
    TATGACAACG TGCTGTGGGC CCTGCTAACG CTGTTCACTG TGTCCACGGG CGAAGGGTGG
    CCCATGGTGC TGAAACACTC GGTGGATGCC ACCTACGAGG AGCAGGGCCC CAGCCCCGGG
    TTCCGCATGG AGCTGTCCAT CTTCTATGTG GTCTACTTCG TGGTCTTCCC CTTCTTCTTT
    GTGAACATCT TTGTGGCCTT GATCATCATC ACCTTCCAGG AGCAGGGGGA CAAAGTCATG
    TCTGAGTGCA GCTTGGAGAA GAACGAGAGG GCTTGTATCG ACTTCGCCAT TAGCGCCAAA
    CCCCTGACGC GGTACATGCC CCAGAACAAG CAGTCGTTCC AGTACAAGAC GTGGACGTTC
    GTGGTCTCAC CACCCTTTGA GTATTTCATC ATGGCCATGA TAGCCCTCAA CACAGTGGTG
    CTGATGATGA AGTTCTACGA TGCGCCCTAC GAGTACGAAC TGATGTTGAA GTGTCTGAAC
    ATCGTGTTCA CGTCCATGTT CTCCATGGAG TGTGTGCTCA AGGTCATCGC CTTTGGAGTG
    CTGAACTATT TCAGAGACGC CTGGAATGTC TTTGACTTTG TCACTGTGTT GGGAAGTATT
    ACTGATATTT TAGTAACAGA GATTGCGAAC AACTTCATCA ACCTCAGCTT CCTCCGCCTC
    TTCCGTGCTG CCCGACTCAT CAAGCTGCTC CGCCAAGGCT ACACCATCCG CATTCTGCTC
    TGGACCTTCG TCCAGTCCTT CAAGGCCCTG CCCTATGTGT GCCTGCTCAT CGCCATGCTG
    TTCTTCATCT ACGCCATCAT CGGCATGCAG GTGTTTGGAA ACATTGCTCT GGATGACGAC
    ACCAGCATCA ACCGTCACAA TAACTTTCGG ACATTTCTTC AGGCCTTGAT GCTGCTCTTC
    AGGAGTGCCA CCGGGGAGGC CTGGCATGAG ATCATGCTGT CCTGCCTCAG CAGTCGGGCC
    TGTGATGAGC TCGCCAATGC CACGGAGTGT GGGAGTGACT TCGCCTACTT CTACTTTGTC
    TCCTTCATCT TCCTTTGCTC CTTTCTGATG TTGAACCTCT TTGTGGCTGT GATCATGGAC
    AATTTTGAGT ACCTTACGCG GGACTCTTCC ATCCTAGGGC CTCACCACCT GGACGAATTC
    ATCCGGGTCT GGGCCGAGTA CGACCCGGCT GCGTGTGGGC GCATCAGTTA CAATGACATG
    TTTGAGATGC TGAAACACAT GTCCCCGCCC CTGGGGCTGG GGAAGAAATG CCCTGCTCGT
    GTTGCGTACA AGCGCCTGGT TCGTATGAAC ATGCCCATCT CCAATGACGA CATGACCGTC
    CACTTCACAT CCACGCTGAT GGCCCTCATC CGGACTGCAT TGGAGATCAA GCTGGCTCCA
    GCTGGCACGA AGCAGCATCA GTGTGACGCA GAGCTGAGGA AGGAGATCTC CTCCGTGTGG
    GCCAATCTGC CCCAGAAGAC CCTGGACTTA CTGGTGCCAC CCCATAAACC CGACGAGATG
    ACGGTGGGGA AGGTGTACGC AGCTCTGATG ATATTCGACT TCTACAAACA GAACAAAACC
    ACCAGAGACC AGATTCACCA AGCTCCTGGA GGCCTGGCCC AGATGGGCCC CGTGTCCCTG
    TTCCACCCCC TGAAGGCCAC GCTGGAGCAG ACTCAGCCGG CTGTGCTCCG AGGAGCCCGC
    GTTTTCCTTC GGCAGAAGAG CTCGGCCTCC CTCAGCAACG GTGGGGCAGT ACAAACCCAA
    GAGGGCGGCA TCAAGGAGTC TGTCTCCTGG GGCACTCAGA GGACCCAGGA GGTCCCCTGC
    GAGGTGAGGA CACCCCTGGA GCGTGGCCAC TCCACAGAGA TCCCGGTGCA GACAGGAAAA
    CCGGCTGTGG ACGTCCAGAT GCAGAGCATG GTCCTGAGAG GCCCTGACGG GGAGCCCCAA
    CCTGGGCTGG AGAGCCAGGG CCGAGCAGCT TCCATGCCCC GCCTGGCAGC AGAGACTCAG
    CCACCCCCAG ATGCCAGCCC CATGAAGCGC TCCATCTCCA CACTGGCACC CCAGAGGCCC
    CACGTGGCTC ATCTGTGCAC CGCGGCCCTG GACCGCGCTC CAGCCAGCCA GGCCGCCCCC
    CCCCACCACC ACCGCTGCCA TCGCCGCAGG GACCGGAAGC AGAAGTCCCT GGAGAAGGGG
    CCCGGCCTGT CTGCAGACAC AGATGGCGCG CCCTACAGCA CCGCGGGGCC GGGCCCCCCG
    CCGCCCCCAG GGGATGGGGC AGCAGGCTGC CGGCGGGAGC GCAGGCAGGA GCGGGGCCGG
    TCCCAGGAGC GGAGGCAGCC CTCCTCCTCT TCCTCTGAGA AGCAGCGCTT CTACTCCTGT
    GACCGCTTTG GGGGCCGTGA GCCCCCGCAG CCCAAGCCCT CTCTCAGCAG CCATCCCACG
    TCGCCCACGG CCGGGCAGGA GCCAGGACCC CCGAGACAGG GTAGCGGCTC CGTGAACGGG
    AGCCCCCTGC TGTCGACATC TGGTGCTAGC ACCCCTGGCC GCGGCGGGCG GAGGCAGCTC
    CCCCAGACCC CGCTGACCCC CCGTCCCAGC ATCACCTATA AGACGGCCAA CTCCTCACCT
    GTCCACTTTG CCGGGGCTCA GACCAGCCTC CCTGCCTTTT CCCCCGGCCG GCTTAGCCGT
    GGCCTTTCGG AACACAACGC CCTGCTCCAG AGAGACCCCC TCAGCCAGCC GCTGGCCCCC
    AGCTCTCGGA TTGGCTCCGA CCCTTACCTG GGGCAGCGTC TGGACAGTGA GGCTGCGGCC
    CGCACCCAGC TTGAGGACAC GCTCACCTTC GAGGAGGCCG TGGCCACCAA CTCGGGCCGC
    TCCTCCCGGA CTTCCTACGT GTCCTCCCTG ACCTCGCAGT CCCACCCCCT CCGCCGGGTG
    CCCAACGGCT ACCACTGCAC TCTGGGGCTC AGCTCGGGCG GGGGCGGCCG GGGGCGGCAC
    AGCTACCACC ACCCTGACCA AGACCACTGG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_777057.1
    CDS126..7121
    Translation

    Target ORF information:

    RefSeq Version NM_174632.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 B (CACNA1B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_174632.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG TGGGGAGCGG 
    GCGCGGGGCG GCGGGGCCGG CGTTGCGGGC GGCCCGGGCC CCGGGGGGCT GCAGCCGGGC
    CAGCGGGTCC TCTACAAGCA GTCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC
    CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG
    GACAACGTCG TCCGCAAATA CGCCAAGCGC ATCACCGAGT GGCCTCCGTT TGAGTACATG
    ATTCTGGCCA CCATCATCGC CAACTGTATC GTGCTGGCTC TGGAGCAGCA CCTCCCAGAC
    GGAGACAAGA CGCCCATGTC TGAGCGGCTG GACGACACGG AGCCCTACTT CATCGGCATC
    TTCTGCTTCG AGGCTGGGAT CAAGATCATC GCGCTGGGCT TTGTCTTGCA CAAGGGCTCC
    TACCTGCGGA ATGGCTGGAA CGTCATGGAC TTCGTGGTGG TCCTCACGGG GATCCTCGCC
    ACAGCTGGCA CTGACTTTGA TCTGCGAACA CTGCGGGCTG TGCGTGTACT GAGGCCCCTG
    AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG
    GTCCCGCTCC TGCAGATTGG GCTGCTTCTC TTTTTCGCCA TCCTCATGTT CGCCATCATT
    GGCCTCGAGT TCTACATGGG CAAATTCCAC AAGGCTTGTT TCCCCAACAG CACAGACGCA
    GATCCGGTGG GTGACTTCCC GTGCGGCAGG GAGGCCCCGG CGCGGCTGTG CGAGGGCGAC
    ACTGAGTGCC GGGAGTACTG GGCGGGCCCC AACTTCGGCA TCACCAACTT CGACAACATC
    CTGTTCGCCA TCCTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACATCCTC
    TACAACACCA ACGACGCGGC TGGCAACACC TGGAACTGGC TCTACTTCCT GCCCCTGATT
    ATCATCGGCT CGTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCAGG AGAGTTTGCC
    AAGGAGCGTG AGCGGGTGGA GAACCGCCGG GCCTTTCTGA AGCTCCGCCG GCAGCAGCAG
    ATCGAGCGGG AGCTCAATGG GTACCTGGAG TGGATCTTCA AGGCCGAGGA GGTCATGCTG
    GCCGAGGAGG ACAAGAACGC AGAGGAGAAG TCCCCTCTGG ATGTGTTGAA GAGAGCAGCC
    ACCAAGAAGA GCCGAAACGA CCTGATCCAC GCGGAGGAGG GAGAGGACCG CTTTGCAGAC
    CTCTGTGCTG TGGGGTCCCC CTTCGCCCGA GCCAGCCTCA AGAGTGGGAA GACGGAGAGC
    TCGTCATACT TCCGCAGGAA GGAGAAGATG TTCCGGTTCT TCATCCGGCG CCTGGTGAAG
    GCGCAGAGCT TCTACTGGGT GGTGCTGTGT GTGGTGGCCC TGAACACGCT GTGCGTGGCC
    ATGGTGCACT ACCAGCAGCC CCAGCGGCTC ACCACGGCGC TGTACTTTGC AGAGTTTGTT
    TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGGAGC
    TACTTCCGGT CCTCCTTCAA CTGCTTCGAT TTCGGGGTCA TTGTGGGGAG CATTTTCGAA
    GTGGTCTGGG CGGCCATCAA GCCAGGAACC TCCTTCGGCA TCAGTGTGCT GCGGGCGCTG
    CGGCTGCTGA GAATCTTCAA AGTCACCAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG
    TCCCTGCTCA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCCTGCTCTT CCTCTTCATC
    GTGGTCTTCG CTCTGCTGGG CATGCAGCTC TTCGGGGGAC AGTTCAACTT CCAGGATGAG
    ACTCCGACAA CCAACTTTGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG
    ACCGGGGAGG ACTGGAATGC GGTGATGTAC CATGGGATCG AATCACAAGG TGGAGTCAGC
    AAGGGCATGT TCTCGTCTGT GTACTTCATT GTCCTGACGC TGTTTGGGAA CTACACCCTG
    CTGAACGTCT TCCTGGCCAT CGCTGTGGAC AACCTCGCCA ATGCTCAGGA GCTGACCAAG
    GACGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA
    GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TTGCTGCCAG GCAGCAGAAC
    TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTGCGGCT GCAGAACCTG
    CGGGCCAGCT GTGAAGCGCT GTATAGCGAG ATGGACCCCG AGGAGCGCCT GCGCTTCGCC
    ACTTCCCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT TGTGGTGGAG
    CCAGGCCGGG ATGGCGCGCG GGGGCCTGCG GGGGGCAAGG CCCGGCCCGA GGGCGGAGAG
    GCCGGCGAGG GTGCCGACCC GCCGCGCAGG CACCACCGGC ACCGCGACAG GGACAGGGAC
    AAGGCCGCAG CCCCGGCGGG GGAGCCAGAC AGGGCGGACG CCCCGAAGGC GGAGGGCGGG
    GAGCCGGGGG CCCGGGAGGA GCGGGCGCGA CCGCGCCGCA GCCGCAGCAA GGAGGCCGCA
    GGCCCCCGCG AGGCCAGGAG CGAGCGAGGC CGCGGGCTGG GCCCGGACGG CGGCCGGCGC
    CACCACCGGC GCGGCTCCCC GGAGGAGGCC GCGGAGCGCG AGCCCCGGCG CCACCGCGCG
    CACCGGCACG CGCCGGAGCC GAGCAGGGAG GGCGCGCCCG GGAGCAAGGG CGAGCGGCGA
    GCGCGGCACC GCGGCGGCTC CCGGGCCGGG CCCCGGGAGG CCGAGAGCGG CGAGGAGCCC
    TCCCGGCGGC ACCGAGCGCG ACACAAGGCG CCGCCCGCAC ACGAGGAGGC GGAGAAGGAG
    GCCGAGGCGG AGGACAGGGA CAAGGAGCCC CGAAACCACC AGCCCAGGGA GTCGCACTGT
    GACCTGGAGG CCAGTGTGGT CATTGGTGTG GGCCCTGTGC ATGCCCTGCC CAGCACTTGT
    CTAGAGAAGG TGGAAGAGCA GCCAGAGGAT GCAGACAATC AGCGGAATGT GACTCGCATG
    GGCAGTCAGC CCTCAGATCT GAGCACCGCT GTGCGTATCC CAGTGACGCT GACCGGGCCT
    CCCGGGGAGA CCACGGTTGT TCCCAGTGGT AACGTGGACC TGGAAAGCCA AGCAGAAGGA
    AAGAAGGAGG TGGAAGCTGA TGACGTGATG AGGAGTGGCC CCCGACCCAT TGTCCCGTAC
    AGCTCCATGT TCTGCTTGAG CCCCACCAAC CTGCTCCGCC GCTTCTGCCA CTACATTGTG
    ACCATGCAGT ACTTCGAGAT GGTCATTCTC GTGGTCATTG CCCTGAGTAG CATTGCCCTG
    GCTGCGGAGG ATCCTGTGCA GACAGACTCG CCCAGGAACA ACGTGCTGAA GTACATGGAC
    TACATCTTCA CAGGTGTCTT CACCTTTGAG ATGGTGATAA AGATGATCGA TTTGGGACTG
    CTGCTGCACC CCGGTGCCTA CTTCCGGGAT CTGTGGAACA TTCTGGACTT CATTGTGGTC
    AGTGGGGCCC TGGTGGCGTT TGCCTTCTCA GGCTCCAAAG GGAAAGACAT CAGCACCATC
    AAGTCCCTGA GGGTCCTGCG TGTGCTGAGG CCACTCAAGA CCATCAAGCG GCTGCCCAAG
    CTCAAAGCTG TGTTTGACTG TGTGGTGAAC TCCCTGAAGA ACGTCCTCAA CATCCTGATC
    GTCTACATGC TCTTCATGTT CATATTTGCT GTCATCGCGG TACAGCTCTT CAAGGGGAAG
    TTTTTCTACT GCACCGACGA GTCCAAGGAG CTGGAGCGGG ACTGCAGGGG CCAGTATTTG
    GACTACGAGA AGGAGGAGGT GGAAGCTCAG CCGCGGCAGT GGAAGAAATA CGACTTCCAC
    TATGACAACG TGCTGTGGGC CCTGCTAACG CTGTTCACTG TGTCCACGGG CGAAGGGTGG
    CCCATGGTGC TGAAACACTC GGTGGATGCC ACCTACGAGG AGCAGGGCCC CAGCCCCGGG
    TTCCGCATGG AGCTGTCCAT CTTCTATGTG GTCTACTTCG TGGTCTTCCC CTTCTTCTTT
    GTGAACATCT TTGTGGCCTT GATCATCATC ACCTTCCAGG AGCAGGGGGA CAAAGTCATG
    TCTGAGTGCA GCTTGGAGAA GAACGAGAGG GCTTGTATCG ACTTCGCCAT TAGCGCCAAA
    CCCCTGACGC GGTACATGCC CCAGAACAAG CAGTCGTTCC AGTACAAGAC GTGGACGTTC
    GTGGTCTCAC CACCCTTTGA GTATTTCATC ATGGCCATGA TAGCCCTCAA CACAGTGGTG
    CTGATGATGA AGTTCTACGA TGCGCCCTAC GAGTACGAAC TGATGTTGAA GTGTCTGAAC
    ATCGTGTTCA CGTCCATGTT CTCCATGGAG TGTGTGCTCA AGGTCATCGC CTTTGGAGTG
    CTGAACTATT TCAGAGACGC CTGGAATGTC TTTGACTTTG TCACTGTGTT GGGAAGTATT
    ACTGATATTT TAGTAACAGA GATTGCGAAC AACTTCATCA ACCTCAGCTT CCTCCGCCTC
    TTCCGTGCTG CCCGACTCAT CAAGCTGCTC CGCCAAGGCT ACACCATCCG CATTCTGCTC
    TGGACCTTCG TCCAGTCCTT CAAGGCCCTG CCCTATGTGT GCCTGCTCAT CGCCATGCTG
    TTCTTCATCT ACGCCATCAT CGGCATGCAG GTGTTTGGAA ACATTGCTCT GGATGACGAC
    ACCAGCATCA ACCGTCACAA TAACTTTCGG ACATTTCTTC AGGCCTTGAT GCTGCTCTTC
    AGGAGTGCCA CCGGGGAGGC CTGGCATGAG ATCATGCTGT CCTGCCTCAG CAGTCGGGCC
    TGTGATGAGC TCGCCAATGC CACGGAGTGT GGGAGTGACT TCGCCTACTT CTACTTTGTC
    TCCTTCATCT TCCTTTGCTC CTTTCTGATG TTGAACCTCT TTGTGGCTGT GATCATGGAC
    AATTTTGAGT ACCTTACGCG GGACTCTTCC ATCCTAGGGC CTCACCACCT GGACGAATTC
    ATCCGGGTCT GGGCCGAGTA CGACCCGGCT GCGTGTGGGC GCATCAGTTA CAATGACATG
    TTTGAGATGC TGAAACACAT GTCCCCGCCC CTGGGGCTGG GGAAGAAATG CCCTGCTCGT
    GTTGCGTACA AGCGCCTGGT TCGTATGAAC ATGCCCATCT CCAATGACGA CATGACCGTC
    CACTTCACAT CCACGCTGAT GGCCCTCATC CGGACTGCAT TGGAGATCAA GCTGGCTCCA
    GCTGGCACGA AGCAGCATCA GTGTGACGCA GAGCTGAGGA AGGAGATCTC CTCCGTGTGG
    GCCAATCTGC CCCAGAAGAC CCTGGACTTA CTGGTGCCAC CCCATAAACC CGACGAGATG
    ACGGTGGGGA AGGTGTACGC AGCTCTGATG ATATTCGACT TCTACAAACA GAACAAAACC
    ACCAGAGACC AGATTCACCA AGCTCCTGGA GGCCTGGCCC AGATGGGCCC CGTGTCCCTG
    TTCCACCCCC TGAAGGCCAC GCTGGAGCAG ACTCAGCCGG CTGTGCTCCG AGGAGCCCGC
    GTTTTCCTTC GGCAGAAGAG CTCGGCCTCC CTCAGCAACG GTGGGGCAGT ACAAACCCAA
    GAGGGCGGCA TCAAGGAGTC TGTCTCCTGG GGCACTCAGA GGACCCAGGA GGTCCCCTGC
    GAGGTGAGGA CACCCCTGGA GCGTGGCCAC TCCACAGAGA TCCCGGTGCA GACAGGAAAA
    CCGGCTGTGG ACGTCCAGAT GCAGAGCATG GTCCTGAGAG GCCCTGACGG GGAGCCCCAA
    CCTGGGCTGG AGAGCCAGGG CCGAGCAGCT TCCATGCCCC GCCTGGCAGC AGAGACTCAG
    CCACCCCCAG ATGCCAGCCC CATGAAGCGC TCCATCTCCA CACTGGCACC CCAGAGGCCC
    CACGTGGCTC ATCTGTGCAC CGCGGCCCTG GACCGCGCTC CAGCCAGCCA GGCCGCCCCC
    CCCCACCACC ACCGCTGCCA TCGCCGCAGG GACCGGAAGC AGAAGTCCCT GGAGAAGGGG
    CCCGGCCTGT CTGCAGACAC AGATGGCGCG CCCTACAGCA CCGCGGGGCC GGGCCCCCCG
    CCGCCCCCAG GGGATGGGGC AGCAGGCTGC CGGCGGGAGC GCAGGCAGGA GCGGGGCCGG
    TCCCAGGAGC GGAGGCAGCC CTCCTCCTCT TCCTCTGAGA AGCAGCGCTT CTACTCCTGT
    GACCGCTTTG GGGGCCGTGA GCCCCCGCAG CCCAAGCCCT CTCTCAGCAG CCATCCCACG
    TCGCCCACGG CCGGGCAGGA GCCAGGACCC CCGAGACAGG GTAGCGGCTC CGTGAACGGG
    AGCCCCCTGC TGTCGACATC TGGTGCTAGC ACCCCTGGCC GCGGCGGGCG GAGGCAGCTC
    CCCCAGACCC CGCTGACCCC CCGTCCCAGC ATCACCTATA AGACGGCCAA CTCCTCACCT
    GTCCACTTTG CCGGGGCTCA GACCAGCCTC CCTGCCTTTT CCCCCGGCCG GCTTAGCCGT
    GGCCTTTCGG AACACAACGC CCTGCTCCAG AGAGACCCCC TCAGCCAGCC GCTGGCCCCC
    AGCTCTCGGA TTGGCTCCGA CCCTTACCTG GGGCAGCGTC TGGACAGTGA GGCTGCGGCC
    CGCACCCAGC TTGAGGACAC GCTCACCTTC GAGGAGGCCG TGGCCACCAA CTCGGGCCGC
    TCCTCCCGGA CTTCCTACGT GTCCTCCCTG ACCTCGCAGT CCCACCCCCT CCGCCGGGTG
    CCCAACGGCT ACCACTGCAC TCTGGGGCTC AGCTCGGGCG GGGGCGGCCG GGGGCGGCAC
    AGCTACCACC ACCCTGACCA AGACCACTGG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.