blm cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following blm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the blm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa53160 XM_012958409.3
Latest version!
Xenopus tropicalis Bloom syndrome RecQ like helicase (blm), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa00753 NM_001256908.1
Latest version!
Xenopus tropicalis Bloom syndrome RecQ like helicase (blm), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30300 XM_012958409.2
Latest version!
Xenopus tropicalis Bloom syndrome, RecQ helicase-like (blm), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa53160
    Clone ID Related Accession (Same CDS sequence) XM_012958409.3
    Accession Version XM_012958409.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Bloom syndrome protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958409.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTGCTC TTCCACAAAA TAATCTTCAG AAGCAGCTAG AACTTTTCTC TGCAAAGGGA 
    ACTGCCAACA AGCTTTCTCT TCAGAAACCA AAATCCAGTG TTTTTACCTT TAAAAAGAAA
    TGTTCTCCAA ATGGTTCATT GTCAACTGGC TTCGTTGAAG TTAAGCAAAA TGTTGCTTTA
    AAGGACAAAA ATGTGAATGT GAAACAAAAT GATGTCCAAA CAAACCTCAC AGTTTTGCCA
    AAATCTGCAG AACGCAATAA AATAAATGGA TTTTTCACCC CGGTCTACAC AAAGTCCACA
    CAGCTGCCAC AGACAGTGGC AGTGAAGGAC CACGTCCATA TCAATGACTC TGCAAACAAG
    CACCCAAGAG TGGAGGATTC TGCATCCAAG AAAACAGGGA TTAATACCTC CTTTGGTTCT
    GTTACCAGTT TGGAGGAATG GGATGATCTC GATGATTTTG ACACATCTGT GTCTCCCCCC
    AAGAGTCAGG CTGGCAAAAT AAAAATAACT CCTCAAAATT GCAAGAACAC ATCTCCTGTT
    TCTTCTAAAA TTCAGTGTCT GAGCCCTAAA GGGCCAACCA TTGAAAAGCA GATGTGTGCC
    AAGAAACCAT ATGAGAATAA TGAGATCGCT TCTGAGCCAC AAAAACTTGT GCATGCTCAA
    ACAGCAGAAA TGCCAGACAA ATCATTAGTT TGTTTGGGGT CTGTGGAACA TACAAATTTA
    GGCAGTGCAG AGAAAGACAC ATGTAGGAAC ATTGATTATT CAGGAACTGA TGATATAGAA
    CATGATCAAG AGACGCTTTC ACAGGCACTT ATTGAAAATG AAGATGATTG TGAACCTGAT
    TTTATTCCCC CATCCCCATC AGATGAAAGT CTGTCCTCTC CTCCGGTTTT AAGAGTAATT
    GGTGCACAAA GGAAACACAA AGTCTCTTCT GTAACAGATG TAAATGACTG TGAAAATACA
    GCTGATCACC TGCAGAGCCA ATGTGTCTCT TCTTCACTTG ATTCCAAAGT GCCTTCTCAT
    CTTTTGAGTG TGATGCTTGA AATCTGTGAC TTGGTTGACA AGATTCCAAT ATCAGAGCTA
    CACGTGCTGT CTTGTGGGAT GGACCTCAAG AAAAAAAGGG ACATGCGGAA GCGCCTCTTG
    TTAAATAACT CTGTGTTAAG ATCATCTCCT GCTGACAGCT CAACAGTATC TTTGACTTCC
    TGTCCATCCC AAAATATGGA ATTTGATGTT AGTGCTCCCA AGGATGCAGA AAGTTTCTCT
    GGATCTTCTG TGAACAAAAT CTTTAAATTC AATAAATTGG TTGCAGACGA GTTTGGCACA
    AAGGGAAATG ATAGGGCTTC TGATTTTGCT CCCAATTTCA GGGAGAAAAT GGAGAATAAA
    ACCTCCTTCT CTCTCCAGGC TGGAGGAGAT TCAGTGATGG AAGACTCCTT CAGTTTTCAG
    AGCAATGTTC TTAGCACCAG CCAGTTTAAT ACCCCACAAA ATGAGAAGCC AGTCTCATCT
    AGCACCTGCG CCAGACCCAT CAGTCAGCCT ATAGATGACA TGGATAACCC TGATCTTGAC
    TTTGATATTG ATAACTTTGA TATTGAGGAC TTTGATGATC AGCTCATGGA CAGCCCTGCA
    GCTCCTCCTG TTTCCAGCAA AAATGCTCCA CAGTACCCAA CAATATGTGA GACACAGTTT
    GATTCTGGAA GTAAAGAGAA AGTTACACGT CATAATGTCG GTGACAAAAC TAACACAGAG
    TTATCAGACT CCCTACTAAA GCCACAGATA GTGAACCCAG CCCTTGAACG ATTCAAAAGC
    TTTAACTTTC CTCACTCCAA GGAAATGATG AAGATTTTTC ACAAGAAATT TGGTTTGCAT
    CGGTTCCGGA CGAACCAGCT AGAAGCAATT AATGCATGCT TGCTTGGGGA AGACTGCTTC
    ATTCTTATGC CAACAGGTGG TGGGAAAAGT CTATGCTACC AACTGCCAGG CTGTGTATCC
    CCAGGAGTCA CAATTGTCAT CTCTCCCCTC CGGTCCCTGA TTGTAGATCA GGTTCAAAAG
    CTCACCTCTT TGGATATTCC AGCTACATAT CTCACTGGAG ATAAGACAGA TGCTGAAGCT
    GCTAGTATTT ATTTGCAACT CTCTAAGAAA GACCCTATCA TTAAACTTCT CTATGTCACT
    CCAGAAAAGG TCTGTGCAAG TACCAGGCTA ATATCAACCA TGGAAAATCT ATATGAGCGT
    CAGTTGCTGG CACGATTTGT CATCGATGAG GCTCACTGTG TCAGTCAGTG GGGCCATGAT
    TTCCGGCCAG ACTACAAGCG TCTGAACATG CTACGCCAGA AGTTTCAGTC TGTACCAATG
    ATGGCTCTCA CAGCAACTGC AAACCCCCGT GTACAGAAGG ATATTCTTAA TCAACTAAAG
    ATGACCAAAC CTCAAATATT TACAATGAGC TTTAACCGAG ACAACCTAAA ATATGACGTA
    CTTCCAAAAA AACCCAAGAG GGTGGCATTG GACTGTGTGG AGTGGATCAA GAAATATCAC
    CCAAATGATT CCGGAATCAT TTATTGTCTG TCACGACATG AGTGTGATAC AATGGCAGAT
    ACCCTGCAGA AGGGGGGCCT TGCTGCACTT GCATATCATG CAGGACTAGC TGACTCTAAC
    AGAGATTATG TACAGCATAA GTGGATAAAC CAAGATGATT GCCAGGTAAT CTGTGCTACC
    ATTGCCTTTG GAATGGGCAT TGATAAGCCA GATGTGAGGT ATGTCATTCA TGCTTCTCTA
    CCAAAGTCTG TGGAGGGATA TTACCAGGAG TCAGGGAGAG CTGGCCGAGA CGGGGAAATA
    TCCCACTGCC TCCTGTTCTA CAGCTACAGT GATGTAACAA GAATTCGTAG GCTAATACAA
    ATGGAGAAAG ATGGAAACAG CCATACAAAA CAGACACATT TTAACAACCT ATACAGTATG
    GTCCACTACT GTGAAAATGT AGTGGAGTGT CGCAGGATGC AACTCCTGGC TTATTTTGGT
    GAAAATAATT TTAATCCAAA CTTCTGCAAA GAGAACACAC GAGTCAGTTG TGATAACTGT
    CTTGGGAGGA AGGAGTTCAA ATCCAGAAAT GTGACCGATG ATGTGAGAAA CATTGTGAGA
    TTTGTGCAAG AAAATTGCTC TTCAGCACGT GGCAAGGGTA GAAGCAACAG CAGTCGACTG
    ACCCTTAACA TGATGGTAGA TATATTCCTA GGTTCTAAGT CTGCAAAAAT CCAAACTGGG
    CTATTTGGGA AAGGAGCTGC TTATTCACGG CACAATGCAG AACGTCTCTT CCGGAAGTTA
    GTCCTGGACA GGATATTAGA TGAAGAGCTT TACATTACTG CCAATGATCA GGCTGTGGCC
    TATGTAAAAA CAGGAGAGCG GGCGCAGGCT GTGCTAAATG GATTCTTGAC GGTTGAGTTC
    CAAGACACCG AAAATGCCTC CAGCCTCAGA AAGCAAAAGG CATCAGTTGT CGCTAATACG
    TCCCAAAGGG AAGAAATGGT CAAGAAGTGC CAGGCAGAGC TCACAGAACT CTGTAAACGC
    CTGGGCAAAA TATTTGGAGT TCATTACTTC AACATCTTCA ACACGGCCAC AATACGGAGA
    ATTGCAGAAT CACTATCTCC TGAGCCTGAT GTGTTACTTC AGATTGATGG TGTTACAGAG
    GATAAGTTGG AGAAATATGG TGCAGAGCTA ATTGATGTTT TACAGAAATA TTCTGAGTGG
    ACACTTCCAG TGGAAGATAT TCACCAAAAA TCTAGTGGGC CTGCCAATTT CTCTGCAAGG
    AGATATGATA GTGATGATGA TGATGATGAT GATGGTGAGA GAGGTGAAAA GTCGTCTTAC
    TTCAGCACCA AGAGTAATAA AGGTACAAAG CGGAAGAATG CCCCCACCTT TAGGAAGTCC
    AAAAAACGAA AGACTGGAGG AGATGGTCAG CAGTCCCATC CCAAGAATGC GACAGGTTCA
    TTTTCTAGGA ATAATTCTAC TGCCAAAACA TCTAGTAGCT ACTACAGTGG TTCAAAGACA
    GGAGCTGATA AAAGACCTGG ATTTATGGCA CCACCATTGC CTCAGCCCAA CAGACGCTTC
    CTTAAACCAT CCTACACTCT GTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813863.2
    CDS438..4544
    Translation

    Target ORF information:

    RefSeq Version XM_012958409.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Bloom syndrome RecQ like helicase (blm), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958409.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTGCTC TTCCACAAAA TAATCTTCAG AAGCAGCTAG AACTTTTCTC TGCAAAGGGA 
    ACTGCCAACA AGCTTTCTCT TCAGAAACCA AAATCCAGTG TTTTTACCTT TAAAAAGAAA
    TGTTCTCCAA ATGGTTCATT GTCAACTGGC TTCGTTGAAG TTAAGCAAAA TGTTGCTTTA
    AAGGACAAAA ATGTGAATGT GAAACAAAAT GATGTCCAAA CAAACCTCAC AGTTTTGCCA
    AAATCTGCAG AACGCAATAA AATAAATGGA TTTTTCACCC CGGTCTACAC AAAGTCCACA
    CAGCTGCCAC AGACAGTGGC AGTGAAGGAC CACGTCCATA TCAATGACTC TGCAAACAAG
    CACCCAAGAG TGGAGGATTC TGCATCCAAG AAAACAGGGA TTAATACCTC CTTTGGTTCT
    GTTACCAGTT TGGAGGAATG GGATGATCTC GATGATTTTG ACACATCTGT GTCTCCCCCC
    AAGAGTCAGG CTGGCAAAAT AAAAATAACT CCTCAAAATT GCAAGAACAC ATCTCCTGTT
    TCTTCTAAAA TTCAGTGTCT GAGCCCTAAA GGGCCAACCA TTGAAAAGCA GATGTGTGCC
    AAGAAACCAT ATGAGAATAA TGAGATCGCT TCTGAGCCAC AAAAACTTGT GCATGCTCAA
    ACAGCAGAAA TGCCAGACAA ATCATTAGTT TGTTTGGGGT CTGTGGAACA TACAAATTTA
    GGCAGTGCAG AGAAAGACAC ATGTAGGAAC ATTGATTATT CAGGAACTGA TGATATAGAA
    CATGATCAAG AGACGCTTTC ACAGGCACTT ATTGAAAATG AAGATGATTG TGAACCTGAT
    TTTATTCCCC CATCCCCATC AGATGAAAGT CTGTCCTCTC CTCCGGTTTT AAGAGTAATT
    GGTGCACAAA GGAAACACAA AGTCTCTTCT GTAACAGATG TAAATGACTG TGAAAATACA
    GCTGATCACC TGCAGAGCCA ATGTGTCTCT TCTTCACTTG ATTCCAAAGT GCCTTCTCAT
    CTTTTGAGTG TGATGCTTGA AATCTGTGAC TTGGTTGACA AGATTCCAAT ATCAGAGCTA
    CACGTGCTGT CTTGTGGGAT GGACCTCAAG AAAAAAAGGG ACATGCGGAA GCGCCTCTTG
    TTAAATAACT CTGTGTTAAG ATCATCTCCT GCTGACAGCT CAACAGTATC TTTGACTTCC
    TGTCCATCCC AAAATATGGA ATTTGATGTT AGTGCTCCCA AGGATGCAGA AAGTTTCTCT
    GGATCTTCTG TGAACAAAAT CTTTAAATTC AATAAATTGG TTGCAGACGA GTTTGGCACA
    AAGGGAAATG ATAGGGCTTC TGATTTTGCT CCCAATTTCA GGGAGAAAAT GGAGAATAAA
    ACCTCCTTCT CTCTCCAGGC TGGAGGAGAT TCAGTGATGG AAGACTCCTT CAGTTTTCAG
    AGCAATGTTC TTAGCACCAG CCAGTTTAAT ACCCCACAAA ATGAGAAGCC AGTCTCATCT
    AGCACCTGCG CCAGACCCAT CAGTCAGCCT ATAGATGACA TGGATAACCC TGATCTTGAC
    TTTGATATTG ATAACTTTGA TATTGAGGAC TTTGATGATC AGCTCATGGA CAGCCCTGCA
    GCTCCTCCTG TTTCCAGCAA AAATGCTCCA CAGTACCCAA CAATATGTGA GACACAGTTT
    GATTCTGGAA GTAAAGAGAA AGTTACACGT CATAATGTCG GTGACAAAAC TAACACAGAG
    TTATCAGACT CCCTACTAAA GCCACAGATA GTGAACCCAG CCCTTGAACG ATTCAAAAGC
    TTTAACTTTC CTCACTCCAA GGAAATGATG AAGATTTTTC ACAAGAAATT TGGTTTGCAT
    CGGTTCCGGA CGAACCAGCT AGAAGCAATT AATGCATGCT TGCTTGGGGA AGACTGCTTC
    ATTCTTATGC CAACAGGTGG TGGGAAAAGT CTATGCTACC AACTGCCAGG CTGTGTATCC
    CCAGGAGTCA CAATTGTCAT CTCTCCCCTC CGGTCCCTGA TTGTAGATCA GGTTCAAAAG
    CTCACCTCTT TGGATATTCC AGCTACATAT CTCACTGGAG ATAAGACAGA TGCTGAAGCT
    GCTAGTATTT ATTTGCAACT CTCTAAGAAA GACCCTATCA TTAAACTTCT CTATGTCACT
    CCAGAAAAGG TCTGTGCAAG TACCAGGCTA ATATCAACCA TGGAAAATCT ATATGAGCGT
    CAGTTGCTGG CACGATTTGT CATCGATGAG GCTCACTGTG TCAGTCAGTG GGGCCATGAT
    TTCCGGCCAG ACTACAAGCG TCTGAACATG CTACGCCAGA AGTTTCAGTC TGTACCAATG
    ATGGCTCTCA CAGCAACTGC AAACCCCCGT GTACAGAAGG ATATTCTTAA TCAACTAAAG
    ATGACCAAAC CTCAAATATT TACAATGAGC TTTAACCGAG ACAACCTAAA ATATGACGTA
    CTTCCAAAAA AACCCAAGAG GGTGGCATTG GACTGTGTGG AGTGGATCAA GAAATATCAC
    CCAAATGATT CCGGAATCAT TTATTGTCTG TCACGACATG AGTGTGATAC AATGGCAGAT
    ACCCTGCAGA AGGGGGGCCT TGCTGCACTT GCATATCATG CAGGACTAGC TGACTCTAAC
    AGAGATTATG TACAGCATAA GTGGATAAAC CAAGATGATT GCCAGGTAAT CTGTGCTACC
    ATTGCCTTTG GAATGGGCAT TGATAAGCCA GATGTGAGGT ATGTCATTCA TGCTTCTCTA
    CCAAAGTCTG TGGAGGGATA TTACCAGGAG TCAGGGAGAG CTGGCCGAGA CGGGGAAATA
    TCCCACTGCC TCCTGTTCTA CAGCTACAGT GATGTAACAA GAATTCGTAG GCTAATACAA
    ATGGAGAAAG ATGGAAACAG CCATACAAAA CAGACACATT TTAACAACCT ATACAGTATG
    GTCCACTACT GTGAAAATGT AGTGGAGTGT CGCAGGATGC AACTCCTGGC TTATTTTGGT
    GAAAATAATT TTAATCCAAA CTTCTGCAAA GAGAACACAC GAGTCAGTTG TGATAACTGT
    CTTGGGAGGA AGGAGTTCAA ATCCAGAAAT GTGACCGATG ATGTGAGAAA CATTGTGAGA
    TTTGTGCAAG AAAATTGCTC TTCAGCACGT GGCAAGGGTA GAAGCAACAG CAGTCGACTG
    ACCCTTAACA TGATGGTAGA TATATTCCTA GGTTCTAAGT CTGCAAAAAT CCAAACTGGG
    CTATTTGGGA AAGGAGCTGC TTATTCACGG CACAATGCAG AACGTCTCTT CCGGAAGTTA
    GTCCTGGACA GGATATTAGA TGAAGAGCTT TACATTACTG CCAATGATCA GGCTGTGGCC
    TATGTAAAAA CAGGAGAGCG GGCGCAGGCT GTGCTAAATG GATTCTTGAC GGTTGAGTTC
    CAAGACACCG AAAATGCCTC CAGCCTCAGA AAGCAAAAGG CATCAGTTGT CGCTAATACG
    TCCCAAAGGG AAGAAATGGT CAAGAAGTGC CAGGCAGAGC TCACAGAACT CTGTAAACGC
    CTGGGCAAAA TATTTGGAGT TCATTACTTC AACATCTTCA ACACGGCCAC AATACGGAGA
    ATTGCAGAAT CACTATCTCC TGAGCCTGAT GTGTTACTTC AGATTGATGG TGTTACAGAG
    GATAAGTTGG AGAAATATGG TGCAGAGCTA ATTGATGTTT TACAGAAATA TTCTGAGTGG
    ACACTTCCAG TGGAAGATAT TCACCAAAAA TCTAGTGGGC CTGCCAATTT CTCTGCAAGG
    AGATATGATA GTGATGATGA TGATGATGAT GATGGTGAGA GAGGTGAAAA GTCGTCTTAC
    TTCAGCACCA AGAGTAATAA AGGTACAAAG CGGAAGAATG CCCCCACCTT TAGGAAGTCC
    AAAAAACGAA AGACTGGAGG AGATGGTCAG CAGTCCCATC CCAAGAATGC GACAGGTTCA
    TTTTCTAGGA ATAATTCTAC TGCCAAAACA TCTAGTAGCT ACTACAGTGG TTCAAAGACA
    GGAGCTGATA AAAGACCTGG ATTTATGGCA CCACCATTGC CTCAGCCCAA CAGACGCTTC
    CTTAAACCAT CCTACACTCT GTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa00753
    Clone ID Related Accession (Same CDS sequence) NM_001256908.1
    Accession Version NM_001256908.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Bloom syndrome protein
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX887827.1, DT415557.1, CU024956.1, AAMC03012790.1, CX923823.1, CT025279.2, DR849961.1 and CX907370.1. On Mar 8, 2012 this sequence version replaced XM_002932953.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN04027316, SAMN04027317 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCTGCTC TTCCACAAAA TAATCTTCAG AAGCAGCTAG AACTTTTCTC TGCAAAGGGA 
    ACTGCCAACA AGCTTTCTCT TCAGAAACCA AAATCCAGTG TTTTTACCTT TAAAAAGAAA
    TGTTCTCCAA ATGGTTCATT GTCAACTGGC TTCGTTGAAG TTAAGCAAAA TGTTGCTTTA
    AAGGACAAAA ATGTGAATGT GAAACAAAAT GATGTCCAAA CAAACCTCAC AGTTTTGCCA
    AAATCTGCAG AACGCAATAA AATAAATGGA TTTTTCACCC CGGTCTACAC AAAGTCCACA
    CAGCTGCCAC AGACAGTGGC AGTGAAGGAC CACGTCCATA TCAATGACTC TGCAAACAAG
    CACCCAAGAG TGGAGGATTC TGCATCCAAG AAAACAGGGA TTAATACCTC CTTTGGTTCT
    GTTACCAGTT TGGAGGAATG GGATGATCTT GATGATTTTG ACACATCTGT GTCTCCCCCC
    AAGAGTCAGG CTGGCAAAAT AAAAATTACT CCTCAAAATT GCAAGAACAC ATCTCCTGTT
    TCTTCTAAAA TTCAGTGTCT GAGCCCTAAA GGGCCAACCA TTGAAAAGCA GATGTGTGCC
    AAGAAACCAT ATGAGAATAA TGAGATCGCT TCTGAGCCAC AAAAACTTGT GCATGCTCAA
    ACAGCAGAAA TGCCAGACAA ATCATTAGTT TGTTTGGGGT CTGTGGAACA TACAAATTTA
    GGCAGTGCAG AGAAAGACAC ATGTAGGAAC ATTGATTATT CAGGAACTGA TGATATAGAA
    CATGATCAAG AGACGCTTTC ACAGGCACTT ATTGAAAATG AAGATGATTG TGAACCTGAT
    TTTATTCCCC CATCCCCATC AGATGAAAGT CTGTCCTCTC CTCCGGTTTT AAGAGTAATT
    GGTGCACAAA GGAAACACAA AGTCTCTTCT GTAACAGATG TAAATGACTG TGAAAATACA
    GCTGATCACC TGCAGAGCCA ATGTGTCTCT TCTTCACTTG ATTCCAAAGT GCCTTCTCAT
    CTTTTGAGTG TGATGCTTGA AATCTGTGAC TTGGTTGACA AGATTCCAAT ATCAGAGCTA
    CACGTGCTGT CTTGTGGGAT GGACCTCAAG AAAAAAAGGG ACATGCGGAA GCGCCTCTTG
    TTAAATAACT CTGTGTTAAG ATCATCTCCT GCTGACAGCT CAACAGTATC TTTGACTTCC
    TGTCCATCCC AAAATATGGA ATTTGATGTT AGTGCTCCCA AGGATTCAGA AAATTTCTCT
    GGATCTTCTG TGAACAAAAT CTTTAAATTC AATAAATTGG TTGCAGACGA GTTTGGCACA
    AAGGGAAATG ATAGGGCTTC TGATTTTGCT CCCAATTTCA GGGAGAAAAT GGAGAATAAA
    ACCTCTTTCT CTCTCCAGGC TGGAGGAGAT TCAGTGATGG AAGACTCCTT CAGTTTTCAG
    AGCAATGTTC TTAGCACCAG CCAGTTTAAT ACCCCACAAA ATGAGAAGCC AGTCTCATCT
    AGCACCTGCG CCAGACCCAT CAGTCAGCCT ATAGATGACA TGGATAACCC TGATCTTGAC
    TTTGATATTG ATAACTTTGA TATTGAGGAC TTTGATGATC AGCTCATGGA CAGCCCTGCA
    GCTCCTCCTG TTTCCAGCAA AAATGCTCCA CAGTACCCAA CAATATGTGA GACACAGTTT
    GATTCTGGAA GTAAAGAGAA AGTTACACGT CATAATGTCG GTGACAAAAC TAACACAGAG
    TTATCAGACT CTCTACTAAA GCCACAGATA GTGAACCCAG CCCTTGAACG ATTCAAAAGC
    TTTAACTTTC CTCACTCCAA GGAAATGATG AAGATTTTTC ACAAGAAATT TGGTTTGCAT
    CGGTTCCGGA CGAACCAGCT AGAAGCAATT AATGCATGCT TGCTTGGGGA AGACTGCTTC
    ATTCTTATGC CAACAGGTGG TGGGAAAAGT CTATGCTACC AACTGCCAGG CTGTGTATCC
    CCAGGAGTCA CAATTGTCAT CTCTCCCCTC CGGTCCCTGA TTGTAGATCA GGTTCAAAAG
    CTCACCTCTT TGGATGTCTG TGCAAGTACC AGGCTAATAT CAACCATGGA AAATCTATAT
    GAGCGTCAGT TGCTGGCACG ATTTGTCATC GATGAGGCTC ACTGTGTCAG TCAGTGGGGC
    CATGATTTCC GGCCAGACTA CAAGCGTCTG AACATGCTAC GCCAGAAGTT TCAGTCTGTA
    CCAATGATGG CTCTCACAGC AACTGCAAAC CCCCGTGTAC AGAAGGATAT TCTTAATCAA
    CTAAAGATGA CCAAACCTCA AATATTTACA ATGAGCTTTA ACCGAGACAA CCTAAAATAT
    GACGTACTTC CAAAAAAACC CAAGAGGGTG GCATTGGACT GTGTGGAGTG GATCAAGAAA
    TATCACCCAA ATGATTCCGG AATCATTTAT TGTCTGTCAC GACATGAGTG TGATACAATG
    GCAGATACCC TGCAGAAGGG GGGCCTTGCT GCACTTGCAT ATCATGCAGG ACTAGCTGAC
    TCTAACAGAG ATTATGTACA GCATAAGTGG ATAAACCAAG ATGATTGCCA GGTAATCTGT
    GCTACCATTG CCTTTGGAAT GGGCATTGAT AAGCCAGATG TGAGGTATGT CATTCATGCT
    TCTCTACCAA AGTCTGTGGA GGGATATTAC CAGGAGTCAG GGAGAGCTGG CCGAGACGGG
    GAAATATCCC ACTGCCTCCT GTTCTACAGC TACAGTGATG TAACAAGAAT TCGTAGGCTA
    ATACAAATGG AGAAAGATGG AAACAGCCAT ACAAAACAGA CACATTTTAA CAACCTATAC
    AGTATGGTCC ACTACTGTGA AAATGTAGTG GAGTGTCGCA GGATGCAACT CCTGGCTTAT
    TTTGGTGAAA ATAATTTTAA TCCAAACTTC TGCAAAGAGA ACACACGAGT CAGTTGTGAT
    AACTGTCTTG GGAGGAAGGA GTTCAAATCC AGAAATGTGA CCGATGATGT GAGAAACATT
    GTGAGATTTG TGCAAGAAAA TTGCTCTTCA GCACGTGGCA AGGGTAGAAG CAACAGCAGT
    CGACTGACCC TTAACATGAT GGTAGATATA TTCCTAGGTT CTAAGTCTGC AAAAATCCAA
    ACTGGGCTAT TTGGGAAAGG AGCTGCTTAT TCACGGCACA ATGCAGAACG TCTCTTCCGG
    AAGTTAGTCC TGGACAGGAT ATTAGATGAA GAGCTTTACA TTACTGCCAA TGATCAGGCT
    GTGGCCTATG TAAAAACAGG AGAGCGGGCG CAGGCTGTGC TAAATGGATT CTTGACGGTT
    GAGTTCCAAG ACACCGAAAA TGCCTCCAGC CTCAGAAAGC AAAAGGCATC AGTTGTCGCT
    AATACGTCCC AAAGGGAAGA AATGGTCAAG AAGTGCCAGG CAGAGCTCAC AGAACTCTGT
    AAACGCCTGG GCAAAATATT TGGAGTTCAT TACTTCAACA TCTTCAACAC GGCCACAATA
    CGGAGAATTG CAGAATCACT ATCTCCTGAG CCTGATGTGT TACTTCAGAT TGATGGTGTT
    ACAGAGGATA AGTTGGAGAA ATATGGTGCA GAGCTAATTG ATGTTTTACA GAAATATTCT
    GAGTGGACAC TTCCAGTGGA AGATATTCAC CAAAAATCTA GTGGGCCTGC CAATTTCTCT
    GCAAGGAGAT ATGATAGTGA TGATGATGAT GGTGAGAGAG GTGAAAAGTC GTCTTACTTC
    AGCACCAAGA GTAATAAAGG TACAAAGCGG AAGAATGCCC CCACCTTTAG GAAGTCCAAA
    AAACGAAAGA CTGGAGGAGA TGGTCAGCAG TCCCATCCCA AGAATGCGAC AGGTTCATTT
    TCTAGGAATA ATTCTACTGC CAAAACATCT AGTAGCTACT ACAGTGGTTC AAAGACAGGA
    GCTGATAAAA GACCTGGATT TATGGCACCA CCATTGCCTC AGCCCAACAG ACGCTTCCTT
    AAACCATCCT ACACTCTGTT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243837.1
    CDS443..4426
    Translation

    Target ORF information:

    RefSeq Version NM_001256908.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Bloom syndrome RecQ like helicase (blm), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256908.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCTGCTC TTCCACAAAA TAATCTTCAG AAGCAGCTAG AACTTTTCTC TGCAAAGGGA 
    ACTGCCAACA AGCTTTCTCT TCAGAAACCA AAATCCAGTG TTTTTACCTT TAAAAAGAAA
    TGTTCTCCAA ATGGTTCATT GTCAACTGGC TTCGTTGAAG TTAAGCAAAA TGTTGCTTTA
    AAGGACAAAA ATGTGAATGT GAAACAAAAT GATGTCCAAA CAAACCTCAC AGTTTTGCCA
    AAATCTGCAG AACGCAATAA AATAAATGGA TTTTTCACCC CGGTCTACAC AAAGTCCACA
    CAGCTGCCAC AGACAGTGGC AGTGAAGGAC CACGTCCATA TCAATGACTC TGCAAACAAG
    CACCCAAGAG TGGAGGATTC TGCATCCAAG AAAACAGGGA TTAATACCTC CTTTGGTTCT
    GTTACCAGTT TGGAGGAATG GGATGATCTT GATGATTTTG ACACATCTGT GTCTCCCCCC
    AAGAGTCAGG CTGGCAAAAT AAAAATTACT CCTCAAAATT GCAAGAACAC ATCTCCTGTT
    TCTTCTAAAA TTCAGTGTCT GAGCCCTAAA GGGCCAACCA TTGAAAAGCA GATGTGTGCC
    AAGAAACCAT ATGAGAATAA TGAGATCGCT TCTGAGCCAC AAAAACTTGT GCATGCTCAA
    ACAGCAGAAA TGCCAGACAA ATCATTAGTT TGTTTGGGGT CTGTGGAACA TACAAATTTA
    GGCAGTGCAG AGAAAGACAC ATGTAGGAAC ATTGATTATT CAGGAACTGA TGATATAGAA
    CATGATCAAG AGACGCTTTC ACAGGCACTT ATTGAAAATG AAGATGATTG TGAACCTGAT
    TTTATTCCCC CATCCCCATC AGATGAAAGT CTGTCCTCTC CTCCGGTTTT AAGAGTAATT
    GGTGCACAAA GGAAACACAA AGTCTCTTCT GTAACAGATG TAAATGACTG TGAAAATACA
    GCTGATCACC TGCAGAGCCA ATGTGTCTCT TCTTCACTTG ATTCCAAAGT GCCTTCTCAT
    CTTTTGAGTG TGATGCTTGA AATCTGTGAC TTGGTTGACA AGATTCCAAT ATCAGAGCTA
    CACGTGCTGT CTTGTGGGAT GGACCTCAAG AAAAAAAGGG ACATGCGGAA GCGCCTCTTG
    TTAAATAACT CTGTGTTAAG ATCATCTCCT GCTGACAGCT CAACAGTATC TTTGACTTCC
    TGTCCATCCC AAAATATGGA ATTTGATGTT AGTGCTCCCA AGGATTCAGA AAATTTCTCT
    GGATCTTCTG TGAACAAAAT CTTTAAATTC AATAAATTGG TTGCAGACGA GTTTGGCACA
    AAGGGAAATG ATAGGGCTTC TGATTTTGCT CCCAATTTCA GGGAGAAAAT GGAGAATAAA
    ACCTCTTTCT CTCTCCAGGC TGGAGGAGAT TCAGTGATGG AAGACTCCTT CAGTTTTCAG
    AGCAATGTTC TTAGCACCAG CCAGTTTAAT ACCCCACAAA ATGAGAAGCC AGTCTCATCT
    AGCACCTGCG CCAGACCCAT CAGTCAGCCT ATAGATGACA TGGATAACCC TGATCTTGAC
    TTTGATATTG ATAACTTTGA TATTGAGGAC TTTGATGATC AGCTCATGGA CAGCCCTGCA
    GCTCCTCCTG TTTCCAGCAA AAATGCTCCA CAGTACCCAA CAATATGTGA GACACAGTTT
    GATTCTGGAA GTAAAGAGAA AGTTACACGT CATAATGTCG GTGACAAAAC TAACACAGAG
    TTATCAGACT CTCTACTAAA GCCACAGATA GTGAACCCAG CCCTTGAACG ATTCAAAAGC
    TTTAACTTTC CTCACTCCAA GGAAATGATG AAGATTTTTC ACAAGAAATT TGGTTTGCAT
    CGGTTCCGGA CGAACCAGCT AGAAGCAATT AATGCATGCT TGCTTGGGGA AGACTGCTTC
    ATTCTTATGC CAACAGGTGG TGGGAAAAGT CTATGCTACC AACTGCCAGG CTGTGTATCC
    CCAGGAGTCA CAATTGTCAT CTCTCCCCTC CGGTCCCTGA TTGTAGATCA GGTTCAAAAG
    CTCACCTCTT TGGATGTCTG TGCAAGTACC AGGCTAATAT CAACCATGGA AAATCTATAT
    GAGCGTCAGT TGCTGGCACG ATTTGTCATC GATGAGGCTC ACTGTGTCAG TCAGTGGGGC
    CATGATTTCC GGCCAGACTA CAAGCGTCTG AACATGCTAC GCCAGAAGTT TCAGTCTGTA
    CCAATGATGG CTCTCACAGC AACTGCAAAC CCCCGTGTAC AGAAGGATAT TCTTAATCAA
    CTAAAGATGA CCAAACCTCA AATATTTACA ATGAGCTTTA ACCGAGACAA CCTAAAATAT
    GACGTACTTC CAAAAAAACC CAAGAGGGTG GCATTGGACT GTGTGGAGTG GATCAAGAAA
    TATCACCCAA ATGATTCCGG AATCATTTAT TGTCTGTCAC GACATGAGTG TGATACAATG
    GCAGATACCC TGCAGAAGGG GGGCCTTGCT GCACTTGCAT ATCATGCAGG ACTAGCTGAC
    TCTAACAGAG ATTATGTACA GCATAAGTGG ATAAACCAAG ATGATTGCCA GGTAATCTGT
    GCTACCATTG CCTTTGGAAT GGGCATTGAT AAGCCAGATG TGAGGTATGT CATTCATGCT
    TCTCTACCAA AGTCTGTGGA GGGATATTAC CAGGAGTCAG GGAGAGCTGG CCGAGACGGG
    GAAATATCCC ACTGCCTCCT GTTCTACAGC TACAGTGATG TAACAAGAAT TCGTAGGCTA
    ATACAAATGG AGAAAGATGG AAACAGCCAT ACAAAACAGA CACATTTTAA CAACCTATAC
    AGTATGGTCC ACTACTGTGA AAATGTAGTG GAGTGTCGCA GGATGCAACT CCTGGCTTAT
    TTTGGTGAAA ATAATTTTAA TCCAAACTTC TGCAAAGAGA ACACACGAGT CAGTTGTGAT
    AACTGTCTTG GGAGGAAGGA GTTCAAATCC AGAAATGTGA CCGATGATGT GAGAAACATT
    GTGAGATTTG TGCAAGAAAA TTGCTCTTCA GCACGTGGCA AGGGTAGAAG CAACAGCAGT
    CGACTGACCC TTAACATGAT GGTAGATATA TTCCTAGGTT CTAAGTCTGC AAAAATCCAA
    ACTGGGCTAT TTGGGAAAGG AGCTGCTTAT TCACGGCACA ATGCAGAACG TCTCTTCCGG
    AAGTTAGTCC TGGACAGGAT ATTAGATGAA GAGCTTTACA TTACTGCCAA TGATCAGGCT
    GTGGCCTATG TAAAAACAGG AGAGCGGGCG CAGGCTGTGC TAAATGGATT CTTGACGGTT
    GAGTTCCAAG ACACCGAAAA TGCCTCCAGC CTCAGAAAGC AAAAGGCATC AGTTGTCGCT
    AATACGTCCC AAAGGGAAGA AATGGTCAAG AAGTGCCAGG CAGAGCTCAC AGAACTCTGT
    AAACGCCTGG GCAAAATATT TGGAGTTCAT TACTTCAACA TCTTCAACAC GGCCACAATA
    CGGAGAATTG CAGAATCACT ATCTCCTGAG CCTGATGTGT TACTTCAGAT TGATGGTGTT
    ACAGAGGATA AGTTGGAGAA ATATGGTGCA GAGCTAATTG ATGTTTTACA GAAATATTCT
    GAGTGGACAC TTCCAGTGGA AGATATTCAC CAAAAATCTA GTGGGCCTGC CAATTTCTCT
    GCAAGGAGAT ATGATAGTGA TGATGATGAT GGTGAGAGAG GTGAAAAGTC GTCTTACTTC
    AGCACCAAGA GTAATAAAGG TACAAAGCGG AAGAATGCCC CCACCTTTAG GAAGTCCAAA
    AAACGAAAGA CTGGAGGAGA TGGTCAGCAG TCCCATCCCA AGAATGCGAC AGGTTCATTT
    TCTAGGAATA ATTCTACTGC CAAAACATCT AGTAGCTACT ACAGTGGTTC AAAGACAGGA
    GCTGATAAAA GACCTGGATT TATGGCACCA CCATTGCCTC AGCCCAACAG ACGCTTCCTT
    AAACCATCCT ACACTCTGTT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30300
    Clone ID Related Accession (Same CDS sequence) XM_012958409.2
    Accession Version XM_012958409.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Bloom syndrome protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTGCTC TTCCACAAAA TAATCTTCAG AAGCAGCTAG AACTTTTCTC TGCAAAGGGA 
    ACTGCCAACA AGCTTTCTCT TCAGAAACCA AAATCCAGTG TTTTTACCTT TAAAAAGAAA
    TGTTCTCCAA ATGGTTCATT GTCAACTGGC TTCGTTGAAG TTAAGCAAAA TGTTGCTTTA
    AAGGACAAAA ATGTGAATGT GAAACAAAAT GATGTCCAAA CAAACCTCAC AGTTTTGCCA
    AAATCTGCAG AACGCAATAA AATAAATGGA TTTTTCACCC CGGTCTACAC AAAGTCCACA
    CAGCTGCCAC AGACAGTGGC AGTGAAGGAC CACGTCCATA TCAATGACTC TGCAAACAAG
    CACCCAAGAG TGGAGGATTC TGCATCCAAG AAAACAGGGA TTAATACCTC CTTTGGTTCT
    GTTACCAGTT TGGAGGAATG GGATGATCTT GATGATTTTG ACACATCTGT GTCTCCCCCC
    AAGAGTCAGG CTGGCAAAAT AAAAATTACT CCTCAAAATT GCAAGAACAC ATCTCCTGTT
    TCTTCTAAAA TTCAGTGTCT GAGCCCTAAA GGGCCAACCA TTGAAAAGCA GATGTGTGCC
    AAGAAACCAT ATGAGAATAA TGAGATCGCT TCTGAGCCAC AAAAACTTGT GCATGCTCAA
    ACAGCAGAAA TGCCAGACAA ATCATTAGTT TGTTTGGGGT CTGTGGAACA TACAAATTTA
    GGCAGTGCAG AGAAAGACAC ATGTAGGAAC ATTGATTATT CAGGAACTGA TGATATAGAA
    CATGATCAAG AGACGCTTTC ACAGGCACTT ATTGAAAATG AAGATGATTG TGAACCTGAT
    TTTATTCCCC CATCCCCATC AGATGAAAGT CTGTCCTCTC CTCCGGTTTT AAGAGTAATT
    GGTGCACAAA GGAAACACAA AGTCTCTTCT GTAACAGATG TAAATGACTG TGAAAATACA
    GCTGATCACC TGCAGAGCCA ATGTGTCTCT TCTTCACTTG ATTCCAAAGT GCCTTCTCAT
    CTTTTGAGTG TGATGCTTGA AATCTGTGAC TTGGTTGACA AGATTCCAAT ATCAGAGCTA
    CACGTGCTGT CTTGTGGGAT GGACCTCAAG AAAAAAAGGG ACATGCGGAA GCGCCTCTTG
    TTAAATAACT CTGTGTTAAG ATCATCTCCT GCTGACAGCT CAACAGTATC TTTGACTTCC
    TGTCCATCCC AAAATATGGA ATTTGATGTT AGTGCTCCCA AGGATTCAGA AAATTTCTCT
    GGATCTTCTG TGAACAAAAT CTTTAAATTC AATAAATTGG TTGCAGACGA GTTTGGCACA
    AAGGGAAATG ATAGGGCTTC TGATTTTGCT CCCAATTTCA GGGAGAAAAT GGAGAATAAA
    ACCTCTTTCT CTCTCCAGGC TGGAGGAGAT TCAGTGATGG AAGACTCCTT CAGTTTTCAG
    AGCAATGTTC TTAGCACCAG CCAGTTTAAT ACCCCACAAA ATGAGAAGCC AGTCTCATCT
    AGCACCTGCG CCAGACCCAT CAGTCAGCCT ATAGATGACA TGGATAACCC TGATCTTGAC
    TTTGATATTG ATAACTTTGA TATTGAGGAC TTTGATGATC AGCTCATGGA CAGCCCTGCA
    GCTCCTCCTG TTTCCAGCAA AAATGCTCCA CAGTACCCAA CAATATGTGA GACACAGTTT
    GATTCTGGAA GTAAAGAGAA AGTTACACGT CATAATGTCG GTGACAAAAC TAACACAGAG
    TTATCAGACT CTCTACTAAA GCCACAGATA GTGAACCCAG CCCTTGAACG ATTCAAAAGC
    TTTAACTTTC CTCACTCCAA GGAAATGATG AAGATTTTTC ACAAGAAATT TGGTTTGCAT
    CGGTTCCGGA CGAACCAGCT AGAAGCAATT AATGCATGCT TGCTTGGGGA AGACTGCTTC
    ATTCTTATGC CAACAGGTGG TGGGAAAAGT CTATGCTACC AACTGCCAGG CTGTGTATCC
    CCAGGAGTCA CAATTGTCAT CTCTCCCCTC CGGTCCCTGA TTGTAGATCA GGTTCAAAAG
    CTCACCTCTT TGGATATTCC AGCTACATAT CTCACTGGAG ATAAGACAGA TGCTGAAGCT
    GCTAGTATTT ATTTGCAACT CTCTAAGAAA GACCCTATCA TTAAACTTCT CTATGTCACT
    CCAGAAAAGG TCTGTGCAAG TACCAGGCTA ATATCAACCA TGGAAAATCT ATATGAGCGT
    CAGTTGCTGG CACGATTTGT CATCGATGAG GCTCACTGTG TCAGTCAGTG GGGCCATGAT
    TTCCGGCCAG ACTACAAGCG TCTGAACATG CTACGCCAGA AGTTTCAGTC TGTACCAATG
    ATGGCTCTCA CAGCAACTGC AAACCCCCGT GTACAGAAGG ATATTCTTAA TCAACTAAAG
    ATGACCAAAC CTCAAATATT TACAATGAGC TTTAACCGAG ACAACCTAAA ATATGACGTA
    CTTCCAAAAA AACCCAAGAG GGTGGCATTG GACTGTGTGG AGTGGATCAA GAAATATCAC
    CCAAATGATT CCGGAATCAT TTATTGTCTG TCACGACATG AGTGTGATAC AATGGCAGAT
    ACCCTGCAGA AGGGGGGCCT TGCTGCACTT GCATATCATG CAGGACTAGC TGACTCTAAC
    AGAGATTATG TACAGCATAA GTGGATAAAC CAAGATGATT GCCAGGTAAT CTGTGCTACC
    ATTGCCTTTG GAATGGGCAT TGATAAGCCA GATGTGAGGT ATGTCATTCA TGCTTCTCTA
    CCAAAGTCTG TGGAGGGATA TTACCAGGAG TCAGGGAGAG CTGGCCGAGA CGGGGAAATA
    TCCCACTGCC TCCTGTTCTA CAGCTACAGT GATGTAACAA GAATTCGTAG GCTAATACAA
    ATGGAGAAAG ATGGAAACAG CCATACAAAA CAGACACATT TTAACAACCT ATACAGTATG
    GTCCACTACT GTGAAAATGT AGTGGAGTGT CGCAGGATGC AACTCCTGGC TTATTTTGGT
    GAAAATAATT TTAATCCAAA CTTCTGCAAA GAGAACACAC GAGTCAGTTG TGATAACTGT
    CTTGGGAGGA AGGAGTTCAA ATCCAGAAAT GTGACCGATG ATGTGAGAAA CATTGTGAGA
    TTTGTGCAAG AAAATTGCTC TTCAGCACGT GGCAAGGGTA GAAGCAACAG CAGTCGACTG
    ACCCTTAACA TGATGGTAGA TATATTCCTA GGTTCTAAGT CTGCAAAAAT CCAAACTGGG
    CTATTTGGGA AAGGAGCTGC TTATTCACGG CACAATGCAG AACGTCTCTT CCGGAAGTTA
    GTCCTGGACA GGATATTAGA TGAAGAGCTT TACATTACTG CCAATGATCA GGCTGTGGCC
    TATGTAAAAA CAGGAGAGCG GGCGCAGGCT GTGCTAAATG GATTCTTGAC GGTTGAGTTC
    CAAGACACCG AAAATGCCTC CAGCCTCAGA AAGCAAAAGG CATCAGTTGT CGCTAATACG
    TCCCAAAGGG AAGAAATGGT CAAGAAGTGC CAGGCAGAGC TCACAGAACT CTGTAAACGC
    CTGGGCAAAA TATTTGGAGT TCATTACTTC AACATCTTCA ACACGGCCAC AATACGGAGA
    ATTGCAGAAT CACTATCTCC TGAGCCTGAT GTGTTACTTC AGATTGATGG TGTTACAGAG
    GATAAGTTGG AGAAATATGG TGCAGAGCTA ATTGATGTTT TACAGAAATA TTCTGAGTGG
    ACACTTCCAG TGGAAGATAT TCACCAAAAA TCTAGTGGGC CTGCCAATTT CTCTGCAAGG
    AGATATGATA GTGATGATGA TGATGGTGAG AGAGGTGAAA AGTCGTCTTA CTTCAGCACC
    AAGAGTAATA AAGGTACAAA GCGGAAGAAT GCCCCCACCT TTAGGAAGTC CAAAAAACGA
    AAGACTGGAG GAGATGGTCA GCAGTCCCAT CCCAAGAATG CGACAGGTTC ATTTTCTAGG
    AATAATTCTA CTGCCAAAAC ATCTAGTAGC TACTACAGTG GTTCAAAGAC AGGAGCTGAT
    AAAAGACCTG GATTTATGGC ACCACCATTG CCTCAGCCCA ACAGACGCTT CCTTAAACCA
    TCCTACACTC TGTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813863.1
    CDS438..4535
    Translation

    Target ORF information:

    RefSeq Version XM_012958409.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Bloom syndrome, RecQ helicase-like (blm), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958409.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTGCTC TTCCACAAAA TAATCTTCAG AAGCAGCTAG AACTTTTCTC TGCAAAGGGA 
    ACTGCCAACA AGCTTTCTCT TCAGAAACCA AAATCCAGTG TTTTTACCTT TAAAAAGAAA
    TGTTCTCCAA ATGGTTCATT GTCAACTGGC TTCGTTGAAG TTAAGCAAAA TGTTGCTTTA
    AAGGACAAAA ATGTGAATGT GAAACAAAAT GATGTCCAAA CAAACCTCAC AGTTTTGCCA
    AAATCTGCAG AACGCAATAA AATAAATGGA TTTTTCACCC CGGTCTACAC AAAGTCCACA
    CAGCTGCCAC AGACAGTGGC AGTGAAGGAC CACGTCCATA TCAATGACTC TGCAAACAAG
    CACCCAAGAG TGGAGGATTC TGCATCCAAG AAAACAGGGA TTAATACCTC CTTTGGTTCT
    GTTACCAGTT TGGAGGAATG GGATGATCTT GATGATTTTG ACACATCTGT GTCTCCCCCC
    AAGAGTCAGG CTGGCAAAAT AAAAATTACT CCTCAAAATT GCAAGAACAC ATCTCCTGTT
    TCTTCTAAAA TTCAGTGTCT GAGCCCTAAA GGGCCAACCA TTGAAAAGCA GATGTGTGCC
    AAGAAACCAT ATGAGAATAA TGAGATCGCT TCTGAGCCAC AAAAACTTGT GCATGCTCAA
    ACAGCAGAAA TGCCAGACAA ATCATTAGTT TGTTTGGGGT CTGTGGAACA TACAAATTTA
    GGCAGTGCAG AGAAAGACAC ATGTAGGAAC ATTGATTATT CAGGAACTGA TGATATAGAA
    CATGATCAAG AGACGCTTTC ACAGGCACTT ATTGAAAATG AAGATGATTG TGAACCTGAT
    TTTATTCCCC CATCCCCATC AGATGAAAGT CTGTCCTCTC CTCCGGTTTT AAGAGTAATT
    GGTGCACAAA GGAAACACAA AGTCTCTTCT GTAACAGATG TAAATGACTG TGAAAATACA
    GCTGATCACC TGCAGAGCCA ATGTGTCTCT TCTTCACTTG ATTCCAAAGT GCCTTCTCAT
    CTTTTGAGTG TGATGCTTGA AATCTGTGAC TTGGTTGACA AGATTCCAAT ATCAGAGCTA
    CACGTGCTGT CTTGTGGGAT GGACCTCAAG AAAAAAAGGG ACATGCGGAA GCGCCTCTTG
    TTAAATAACT CTGTGTTAAG ATCATCTCCT GCTGACAGCT CAACAGTATC TTTGACTTCC
    TGTCCATCCC AAAATATGGA ATTTGATGTT AGTGCTCCCA AGGATTCAGA AAATTTCTCT
    GGATCTTCTG TGAACAAAAT CTTTAAATTC AATAAATTGG TTGCAGACGA GTTTGGCACA
    AAGGGAAATG ATAGGGCTTC TGATTTTGCT CCCAATTTCA GGGAGAAAAT GGAGAATAAA
    ACCTCTTTCT CTCTCCAGGC TGGAGGAGAT TCAGTGATGG AAGACTCCTT CAGTTTTCAG
    AGCAATGTTC TTAGCACCAG CCAGTTTAAT ACCCCACAAA ATGAGAAGCC AGTCTCATCT
    AGCACCTGCG CCAGACCCAT CAGTCAGCCT ATAGATGACA TGGATAACCC TGATCTTGAC
    TTTGATATTG ATAACTTTGA TATTGAGGAC TTTGATGATC AGCTCATGGA CAGCCCTGCA
    GCTCCTCCTG TTTCCAGCAA AAATGCTCCA CAGTACCCAA CAATATGTGA GACACAGTTT
    GATTCTGGAA GTAAAGAGAA AGTTACACGT CATAATGTCG GTGACAAAAC TAACACAGAG
    TTATCAGACT CTCTACTAAA GCCACAGATA GTGAACCCAG CCCTTGAACG ATTCAAAAGC
    TTTAACTTTC CTCACTCCAA GGAAATGATG AAGATTTTTC ACAAGAAATT TGGTTTGCAT
    CGGTTCCGGA CGAACCAGCT AGAAGCAATT AATGCATGCT TGCTTGGGGA AGACTGCTTC
    ATTCTTATGC CAACAGGTGG TGGGAAAAGT CTATGCTACC AACTGCCAGG CTGTGTATCC
    CCAGGAGTCA CAATTGTCAT CTCTCCCCTC CGGTCCCTGA TTGTAGATCA GGTTCAAAAG
    CTCACCTCTT TGGATATTCC AGCTACATAT CTCACTGGAG ATAAGACAGA TGCTGAAGCT
    GCTAGTATTT ATTTGCAACT CTCTAAGAAA GACCCTATCA TTAAACTTCT CTATGTCACT
    CCAGAAAAGG TCTGTGCAAG TACCAGGCTA ATATCAACCA TGGAAAATCT ATATGAGCGT
    CAGTTGCTGG CACGATTTGT CATCGATGAG GCTCACTGTG TCAGTCAGTG GGGCCATGAT
    TTCCGGCCAG ACTACAAGCG TCTGAACATG CTACGCCAGA AGTTTCAGTC TGTACCAATG
    ATGGCTCTCA CAGCAACTGC AAACCCCCGT GTACAGAAGG ATATTCTTAA TCAACTAAAG
    ATGACCAAAC CTCAAATATT TACAATGAGC TTTAACCGAG ACAACCTAAA ATATGACGTA
    CTTCCAAAAA AACCCAAGAG GGTGGCATTG GACTGTGTGG AGTGGATCAA GAAATATCAC
    CCAAATGATT CCGGAATCAT TTATTGTCTG TCACGACATG AGTGTGATAC AATGGCAGAT
    ACCCTGCAGA AGGGGGGCCT TGCTGCACTT GCATATCATG CAGGACTAGC TGACTCTAAC
    AGAGATTATG TACAGCATAA GTGGATAAAC CAAGATGATT GCCAGGTAAT CTGTGCTACC
    ATTGCCTTTG GAATGGGCAT TGATAAGCCA GATGTGAGGT ATGTCATTCA TGCTTCTCTA
    CCAAAGTCTG TGGAGGGATA TTACCAGGAG TCAGGGAGAG CTGGCCGAGA CGGGGAAATA
    TCCCACTGCC TCCTGTTCTA CAGCTACAGT GATGTAACAA GAATTCGTAG GCTAATACAA
    ATGGAGAAAG ATGGAAACAG CCATACAAAA CAGACACATT TTAACAACCT ATACAGTATG
    GTCCACTACT GTGAAAATGT AGTGGAGTGT CGCAGGATGC AACTCCTGGC TTATTTTGGT
    GAAAATAATT TTAATCCAAA CTTCTGCAAA GAGAACACAC GAGTCAGTTG TGATAACTGT
    CTTGGGAGGA AGGAGTTCAA ATCCAGAAAT GTGACCGATG ATGTGAGAAA CATTGTGAGA
    TTTGTGCAAG AAAATTGCTC TTCAGCACGT GGCAAGGGTA GAAGCAACAG CAGTCGACTG
    ACCCTTAACA TGATGGTAGA TATATTCCTA GGTTCTAAGT CTGCAAAAAT CCAAACTGGG
    CTATTTGGGA AAGGAGCTGC TTATTCACGG CACAATGCAG AACGTCTCTT CCGGAAGTTA
    GTCCTGGACA GGATATTAGA TGAAGAGCTT TACATTACTG CCAATGATCA GGCTGTGGCC
    TATGTAAAAA CAGGAGAGCG GGCGCAGGCT GTGCTAAATG GATTCTTGAC GGTTGAGTTC
    CAAGACACCG AAAATGCCTC CAGCCTCAGA AAGCAAAAGG CATCAGTTGT CGCTAATACG
    TCCCAAAGGG AAGAAATGGT CAAGAAGTGC CAGGCAGAGC TCACAGAACT CTGTAAACGC
    CTGGGCAAAA TATTTGGAGT TCATTACTTC AACATCTTCA ACACGGCCAC AATACGGAGA
    ATTGCAGAAT CACTATCTCC TGAGCCTGAT GTGTTACTTC AGATTGATGG TGTTACAGAG
    GATAAGTTGG AGAAATATGG TGCAGAGCTA ATTGATGTTT TACAGAAATA TTCTGAGTGG
    ACACTTCCAG TGGAAGATAT TCACCAAAAA TCTAGTGGGC CTGCCAATTT CTCTGCAAGG
    AGATATGATA GTGATGATGA TGATGGTGAG AGAGGTGAAA AGTCGTCTTA CTTCAGCACC
    AAGAGTAATA AAGGTACAAA GCGGAAGAAT GCCCCCACCT TTAGGAAGTC CAAAAAACGA
    AAGACTGGAG GAGATGGTCA GCAGTCCCAT CCCAAGAATG CGACAGGTTC ATTTTCTAGG
    AATAATTCTA CTGCCAAAAC ATCTAGTAGC TACTACAGTG GTTCAAAGAC AGGAGCTGAT
    AAAAGACCTG GATTTATGGC ACCACCATTG CCTCAGCCCA ACAGACGCTT CCTTAAACCA
    TCCTACACTC TGTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.