Blm cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Blm
Entrez Gene ID	308755
Full Name	Bloom syndrome RecQ like helicase
General protein information	Preferred Names Bloom syndrome RecQ like helicase Names Bloom syndrome RecQ like helicase Bloom syndrome protein Bloom syndrome homolog Bloom syndrome, RecQ helicase-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q31

mRNA and Protein(s)

mRNA	Protein	Name
XM_003748888.4	XP_003748936.2	Bloom syndrome protein isoform X2
XM_017590166.1	XP_017445655.1	Bloom syndrome protein isoform X4
XM_003753301.4	XP_003753349.2	Bloom syndrome protein isoform X2
XM_006229458.3	XP_006229520.1	Bloom syndrome protein isoform X1
XM_017590165.1	XP_017445654.1	Bloom syndrome protein isoform X3
XM_006223359.3	XP_006223421.1	Bloom syndrome protein isoform X1
XM_017589083.1	XP_017444572.1	Bloom syndrome protein isoform X3
XM_017589087.1	XP_017444576.1	Bloom syndrome protein isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BLM	XP_510594.2
Canis lupus familiaris (dog)	BLM	XP_003434427.1
Mus musculus (house mouse)	Blm	NP_031576.4
	BLM	XP_001097543.1
Gallus gallus (chicken)	BLM	NP_001007088.2
Xenopus tropicalis (tropical clawed frog)	blm	NP_001243837.1
Homo sapiens (human)	BLM	NP_000048.1
Bos taurus (cattle)	BLM	XP_002696629.1
Rattus norvegicus (Norway rat)	Blm	XP_006229520.1
Danio rerio (zebrafish)	blm	XP_701357.4

Gene Ontology

The following Blm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Blm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa53704	XM_017589083.1 Latest version!	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa53705	XM_017589087.1 Latest version!	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa56975	XM_017590165.1 Latest version!	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa56976	XM_017590166.1 Latest version!	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa14498	XM_006223359.3 Latest version!	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa14499	XM_003753301.4 Latest version!	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa22955	XM_006229458.3 Latest version!	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa22956	XM_003748888.4 Latest version!	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Blm ( XM_017589083.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444572.1 Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa53704
Clone ID Related Accession (Same CDS sequence)	XM_017589083.1
Accession Version	XM_017589083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Bloom syndrome protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG TTCCTCTGAA CAATCTACAA GAACAACTAC AGCGTCACTC GGCCAGAAAA 
CTTAAAAATC AGTTCAGCGT TTCAAAACCA AAACCTTCAG GTTTTACTTT TAAAAAGAAA 
ACACCAGAGG GTGATGTGTC TGTGGTAAGA ACACCTGTGT TAAGCGATAA AGATGTGAAT 
ATGTCTGAGG CCTTGTCATA TACTGAGCCT CCACTCCACA AACCAAAGCA GCAGTCAAAC 
ATCGATGGCT TCTTTAAAAA TTCCCCTGGA GGGCAGCAAA GCAAGGAGAC CTGCTCCGAA 
CTGTCCCTGC CGGAGGTGGT ACAGATTCCT CAGGACGCTT CTTGTACTAC CCCAAAAACC 
CCAGCTGTGA AGAAACCCCA CGTGGCCATT TTCAAAAAAC TGGAATTTAG TTCTTCGGCA 
GACTCTCTCA GTGATTGGGC TGATATAGAT GACTTTGATG TGCCAGCATC AGACGCATTT 
GTTTCCCTGA CCAGAAACCC TACTACAAGA GTGAGCACTG CTAAGAAACT GAGTAGCAGT 
AAGAGAAACT TCTTTAAACC ACCACCTAGA GCAAGTGCAG TAAAGGCCGA CTTGACTCCT 
TCCTCCCCCG AATGCCTGCA AGTGGATTTA ACGAAGGAAC AGGAGGAAGA GGCGGACTGC 
CTGAGCAGGG ATGTGATCTG CATTGACACT GACCCTGCTT CTGAGGAGCT CTTGGAGAAG 
GATGTGCAGG AAGGCCTGTC ATTGGACGCT CGCTTGGGGA CTGAAAGAGG TATTGACAGT 
GCAAAGAAGA GCCATGAAGA CGAGGCTGCA TTCCAGTCAC CTCAGAACGC TCTGTACTTT 
GAACACAATG ACAATGATTA TGATATAGAT TTTGTTCCAC CTTCTCCAGA AGAAATCATT 
TCCACTGCTT CTTCCTCGTT GAAATGTTCC AGTATGCTAA AGGATCTCGA TGACTCTGAC 
AAAGAGAAGG ACATTCTGAG TACCTCAAAA GATCTGTCAA AACCAGAGGA AATGACCACA 
CCGAAGTCTG ATCTGGGAAC CAATGAAGAC TGTGATGCCC AGCAGACTAG CTTGCAGCAG 
CAGCTTATCC GTGTGATGGA GCACATCTGT AAATTAGTTG ATACTGTTCC TATTGATGAA 
CTGAAAGCTC TGACTTGTGG GGACAAACTG CTTCAGCAGC GAGACATAAG AAGGAAGCTC 
CTTGCTGAAG CAGGTTTTAA TAGAAACGAT GTCAATCTTC TGGGTTCTTT GTGGAGGCAC 
AGGCCAGATT CGCTTGATAA CACTGTGCAG TGTGACTCCT GTCCTGTGGG GCATCCTGGT 
AAAGAGTTAA ACTCTCCATA CCTTCCCTCA CACTCCCTCT CCACTGAGGA ATGCTTACCC 
ACCACTGCTT CAGGAAAGAC AGGATTCTCA GCCACCCCGA AGAGTCTCTC TGAAAGGCCA 
CCATTGAATT CCCACTTACA GAAGTCCTTT GTAAGTAGCA ACTGGGCTGA AACACCAAGG 
ACAGAAAGCA GGACGGAAAG CACTTACTTC CCAGGGAGTG TTCTCACAAG CACCACTGTG 
AAGGATGAGA GTAAACATGT TGCTTCAGGA CGTGACATAG AGAGAGAAGT CCAGGCTTCC 
TACGATATTG ATAATTTTAA CATTGATGAC TTTGATGATG ATGATGATGA TGACTGGGAA 
AACATAATGC ACAATTTTTC AGTCAGCAAA TCTTCCACAG CTGCCTACCC ACCTATCAAG 
GAAGGTGGCC CAGTTAAATC TCTATCAGAA AGGATTTCTT CAGCCAAGGC AAAGTTTCTT 
CCAGTGGCAT CAACCGCTCA AAATAAAAAC CTCTCAGAGT CAATTCAGAA TTGCTCTGAT 
AAGTTTGCAC AAAATCTATC ATCAAAAAAT CCAAAACATG AACATTTTCA AAGTCTTAAT 
TTTCCTCATA CAAAAGAAAT GATGAAGATT TTCCGTAAGA AATTTGGCTT GCATAATTTT 
AGAACTAATC AGCTAGAGGC GATCAATGCT GCGCTGCTTG GTGAAGACTG CTTTATCCTG 
ATGCCCACTG GAGGTGGTAA AAGTTTGTGC TACCAGCTCC CTGCCTGTGT TTCTCCTGGA 
GTCACTATTG TCATTTCTCC CTTGAGATCA CTAATAGTGG ACCAAGTCCA AAAGCTGACT 
TCCTTTGATA TTCCAGCTAC GTACCTGACA GGGGATAAGA CTGACTCCGA AGCTGCAAAC 
ATTTACCTCC AGTTGTCAAA GAAAGACCCG ATCATCAAGC TTCTGTATGT CACCCCAGAG 
AAGGTCTGTG CAAGTAACAG GCTGATTTCC ACTCTGGAGA ATCTGTATGA GCGGAAGCTC 
TTGGCACGAT TTGTCATTGA TGAAGCACAC TGTGTGAGTC AGTGGGGCCA TGATTTCCGT 
CAAGATTACA AAAGGATGAA TATGCTTCGC CAGAGGTTCC CTTCTGTCCC AGTGATGGCT 
CTCACGGCCA CAGCGAACCC CAGGGTACAG AAGGACATCC TCACGCAGCT GAAGATTCTC 
AGGCCTCAGG TGTTTAGCAT GAGCTTTAAC AGACACAATC TGAAATACTA TGTATTACCA 
AAGAAGCCCA AGAAAGTGGC ACTTGATTGC CTAGAGTGGA TCAGAAAGCA TCACCCTTAT 
GACTCGGGGA TAATTTATTG CCTCTCCAGG AAGGAATGTG ACACAATGGC CGACACCTTA 
CAGAGAGAAG GTCTTGCTGC TCTGGCTTAC CATGCAGGTC TTAGTGATTC TGCCAGAGAT 
GAGGTGCAGC ACAAGTGGAT CAACCAGGAC AACTGCCAGG TTATCTGTGC AACCATTGCA 
TTTGGAATGG GAATCGACAA ACCTGACGTG CGATTTGTGG TTCATGCATC TCTTCCTAAA 
TCTGTGGAGG GTTATTACCA AGAATCCGGC CGAGCTGGGA GAGACGGGGA AATATCTCAC 
TGCGTGCTTT TCTATACCTA TCATGACGTG ACCAGACTGA AGAGACTTAT AATGATGGAA 
AAAGATGGAA ACTATCATAC AAGGGAAACT CACGTCAATA ATTTATATAG CATGGTACAT 
TACTGCGAAA ACATAACAGA ATGCAGAAGA ATACAGCTTT TAGCTTACTT TGGTGAAAAA 
GGATTTAACC CTGATTTTTG TAAGAAACAT CCAGATGTTT CTTGTGACAA TTGCTGTAAA 
ACAAAGGATT ATAAAACAAA AGATGTGACT GATGATGTGA AAAATATTGT AAGATTTGTT 
CAAGAACACA GTTCATCACC AGGAACAAGA AATATAGGAC CTGCTGGAAG ATTTACTCTG 
AACATGCTAG TCGACATTTT CTTGGGAAGT AAGAGTGCAA AAGTTCAGTC TGGAATATTT 
GGAAAGGGGT CTGCATATTC ACGCCACAAT GCTGAAAGAC TTTTTAAAAA GCTGATTCTA 
GACAAAATCT TGGATGAAGA CTTATATATC AATGCCAATG ACCAACCAAT TGCCTATGTG 
ATGCTCGGAA CAAAAGCCCA CAGTGTGCTG AGCGGCCACT TGAAGGTGGA CTTCATGGAA 
ACGGAAAATT CGAGCAGTGT TAAAAAACAA AAAGCCGCAG TGGCCCACGT GTCCCAGAGA 
GACGAGGCAG TTATGAAGTG TCTGGGAGAA CTTGCAGACG TCTGCAAATT GCTGGGCAAA 
GTCTTTGGTG TCCATTACTT CAATATTTTC AATACAGCCA CCCTCAAAAA GCTTGCAGAA 
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAGATTGATG GTGTTACTGA AGACAAGCTG 
GAGAAATATG GTGCAGAAAT AATTCCAGTA TTACAGAAGT ACGCAGAGTG GACATTGCCA 
GCTGAAGGTG GGTCCCCAGG CAGCAGAGGC TCTAGGAGGA GCACCCCAGA GGAGACGGAG 
GAGGAGGAAG CTGTATCTTC TCACTACTTT GCAAATCAAA CTAGAAATGA AAGAAAGAGG 
AAGAAAGTGT CAGCCTCCCA AAAGCCCAAG AGAAGAAGAA CTAGTTACGG CGGCGGCTTC 
AGAGCAAAGG GGGGCTCTAC TACATGCAGA AAACCATCTT CTAAAAGTAA ATTCTCTGGT 
GTAACTGGAT CCCGCTCAGC CTCGTGTGCT TCTCAGGCAA CATCAGCAGC CAGTAGAAAA 
CTGGGGATTA TGGCTCCTCC AAAGCCTGTA AATAGACCGT TCCTCAGGCC TGCGTATGCC 
TTCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444572.1
CDS	123..4331
Translation	MAAVPLNNLQEQLQRHSARKLKNQFSVSKPKPSGFTFKKKTPEGDVSVVRTPVLSDKDVNMSEALSYTEPPLHKPKQQSNIDGFFKNSPGGQQSKETCSELSLPEVVQIPQDASCTTPKTPAVKKPHVAIFKKLEFSSSADSLSDWADIDDFDVPASDAFVSLTRNPTTRVSTAKKLSSSKRNFFKPPPRASAVKADLTPSSPECLQVDLTKEQEEEADCLSRDVICIDTDPASEELLEKDVQEGLSLDARLGTERGIDSAKKSHEDEAAFQSPQNALYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKDILSTSKDLSKPEEMTTPKSDLGTNEDCDAQQTSLQQQLIRVMEHICKLVDTVPIDELKALTCGDKLLQQRDIRRKLLAEAGFNRNDVNLLGSLWRHRPDSLDNTVQCDSCPVGHPGKELNSPYLPSHSLSTEECLPTTASGKTGFSATPKSLSERPPLNSHLQKSFVSSNWAETPRTESRTESTYFPGSVLTSTTVKDESKHVASGRDIEREVQASYDIDNFNIDDFDDDDDDDWENIMHNFSVSKSSTAAYPPIKEGGPVKSLSERISSAKAKFLPVASTAQNKNLSESIQNCSDKFAQNLSSKNPKHEHFQSLNFPHTKEMMKIFRKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQRFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVALDCLEWIRKHHPYDSGIIYCLSRKECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVVHASLPKSVEGYYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTRETHVNNLYSMVHYCENITECRRIQLLAYFGEKGFNPDFCKKHPDVSCDNCCKTKDYKTKDVTDDVKNIVRFVQEHSSSPGTRNIGPAGRFTLNMLVDIFLGSKSAKVQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHLKVDFMETENSSSVKKQKAAVAHVSQRDEAVMKCLGELADVCKLLGKVFGVHYFNIFNTATLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEIIPVLQKYAEWTLPAEGGSPGSRGSRRSTPEETEEEEAVSSHYFANQTRNERKRKKVSASQKPKRRRTSYGGGFRAKGGSTTCRKPSSKSKFSGVTGSRSASCASQATSAASRKLGIMAPPKPVNRPFLRPAYAFS

Target ORF information:

RefSeq Version	XM_017589083.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589083.1

ORF Insert Sequence:

ATGGCTGCTG TTCCTCTGAA CAATCTACAA GAACAACTAC AGCGTCACTC GGCCAGAAAA 
CTTAAAAATC AGTTCAGCGT TTCAAAACCA AAACCTTCAG GTTTTACTTT TAAAAAGAAA 
ACACCAGAGG GTGATGTGTC TGTGGTAAGA ACACCTGTGT TAAGCGATAA AGATGTGAAT 
ATGTCTGAGG CCTTGTCATA TACTGAGCCT CCACTCCACA AACCAAAGCA GCAGTCAAAC 
ATCGATGGCT TCTTTAAAAA TTCCCCTGGA GGGCAGCAAA GCAAGGAGAC CTGCTCCGAA 
CTGTCCCTGC CGGAGGTGGT ACAGATTCCT CAGGACGCTT CTTGTACTAC CCCAAAAACC 
CCAGCTGTGA AGAAACCCCA CGTGGCCATT TTCAAAAAAC TGGAATTTAG TTCTTCGGCA 
GACTCTCTCA GTGATTGGGC TGATATAGAT GACTTTGATG TGCCAGCATC AGACGCATTT 
GTTTCCCTGA CCAGAAACCC TACTACAAGA GTGAGCACTG CTAAGAAACT GAGTAGCAGT 
AAGAGAAACT TCTTTAAACC ACCACCTAGA GCAAGTGCAG TAAAGGCCGA CTTGACTCCT 
TCCTCCCCCG AATGCCTGCA AGTGGATTTA ACGAAGGAAC AGGAGGAAGA GGCGGACTGC 
CTGAGCAGGG ATGTGATCTG CATTGACACT GACCCTGCTT CTGAGGAGCT CTTGGAGAAG 
GATGTGCAGG AAGGCCTGTC ATTGGACGCT CGCTTGGGGA CTGAAAGAGG TATTGACAGT 
GCAAAGAAGA GCCATGAAGA CGAGGCTGCA TTCCAGTCAC CTCAGAACGC TCTGTACTTT 
GAACACAATG ACAATGATTA TGATATAGAT TTTGTTCCAC CTTCTCCAGA AGAAATCATT 
TCCACTGCTT CTTCCTCGTT GAAATGTTCC AGTATGCTAA AGGATCTCGA TGACTCTGAC 
AAAGAGAAGG ACATTCTGAG TACCTCAAAA GATCTGTCAA AACCAGAGGA AATGACCACA 
CCGAAGTCTG ATCTGGGAAC CAATGAAGAC TGTGATGCCC AGCAGACTAG CTTGCAGCAG 
CAGCTTATCC GTGTGATGGA GCACATCTGT AAATTAGTTG ATACTGTTCC TATTGATGAA 
CTGAAAGCTC TGACTTGTGG GGACAAACTG CTTCAGCAGC GAGACATAAG AAGGAAGCTC 
CTTGCTGAAG CAGGTTTTAA TAGAAACGAT GTCAATCTTC TGGGTTCTTT GTGGAGGCAC 
AGGCCAGATT CGCTTGATAA CACTGTGCAG TGTGACTCCT GTCCTGTGGG GCATCCTGGT 
AAAGAGTTAA ACTCTCCATA CCTTCCCTCA CACTCCCTCT CCACTGAGGA ATGCTTACCC 
ACCACTGCTT CAGGAAAGAC AGGATTCTCA GCCACCCCGA AGAGTCTCTC TGAAAGGCCA 
CCATTGAATT CCCACTTACA GAAGTCCTTT GTAAGTAGCA ACTGGGCTGA AACACCAAGG 
ACAGAAAGCA GGACGGAAAG CACTTACTTC CCAGGGAGTG TTCTCACAAG CACCACTGTG 
AAGGATGAGA GTAAACATGT TGCTTCAGGA CGTGACATAG AGAGAGAAGT CCAGGCTTCC 
TACGATATTG ATAATTTTAA CATTGATGAC TTTGATGATG ATGATGATGA TGACTGGGAA 
AACATAATGC ACAATTTTTC AGTCAGCAAA TCTTCCACAG CTGCCTACCC ACCTATCAAG 
GAAGGTGGCC CAGTTAAATC TCTATCAGAA AGGATTTCTT CAGCCAAGGC AAAGTTTCTT 
CCAGTGGCAT CAACCGCTCA AAATAAAAAC CTCTCAGAGT CAATTCAGAA TTGCTCTGAT 
AAGTTTGCAC AAAATCTATC ATCAAAAAAT CCAAAACATG AACATTTTCA AAGTCTTAAT 
TTTCCTCATA CAAAAGAAAT GATGAAGATT TTCCGTAAGA AATTTGGCTT GCATAATTTT 
AGAACTAATC AGCTAGAGGC GATCAATGCT GCGCTGCTTG GTGAAGACTG CTTTATCCTG 
ATGCCCACTG GAGGTGGTAA AAGTTTGTGC TACCAGCTCC CTGCCTGTGT TTCTCCTGGA 
GTCACTATTG TCATTTCTCC CTTGAGATCA CTAATAGTGG ACCAAGTCCA AAAGCTGACT 
TCCTTTGATA TTCCAGCTAC GTACCTGACA GGGGATAAGA CTGACTCCGA AGCTGCAAAC 
ATTTACCTCC AGTTGTCAAA GAAAGACCCG ATCATCAAGC TTCTGTATGT CACCCCAGAG 
AAGGTCTGTG CAAGTAACAG GCTGATTTCC ACTCTGGAGA ATCTGTATGA GCGGAAGCTC 
TTGGCACGAT TTGTCATTGA TGAAGCACAC TGTGTGAGTC AGTGGGGCCA TGATTTCCGT 
CAAGATTACA AAAGGATGAA TATGCTTCGC CAGAGGTTCC CTTCTGTCCC AGTGATGGCT 
CTCACGGCCA CAGCGAACCC CAGGGTACAG AAGGACATCC TCACGCAGCT GAAGATTCTC 
AGGCCTCAGG TGTTTAGCAT GAGCTTTAAC AGACACAATC TGAAATACTA TGTATTACCA 
AAGAAGCCCA AGAAAGTGGC ACTTGATTGC CTAGAGTGGA TCAGAAAGCA TCACCCTTAT 
GACTCGGGGA TAATTTATTG CCTCTCCAGG AAGGAATGTG ACACAATGGC CGACACCTTA 
CAGAGAGAAG GTCTTGCTGC TCTGGCTTAC CATGCAGGTC TTAGTGATTC TGCCAGAGAT 
GAGGTGCAGC ACAAGTGGAT CAACCAGGAC AACTGCCAGG TTATCTGTGC AACCATTGCA 
TTTGGAATGG GAATCGACAA ACCTGACGTG CGATTTGTGG TTCATGCATC TCTTCCTAAA 
TCTGTGGAGG GTTATTACCA AGAATCCGGC CGAGCTGGGA GAGACGGGGA AATATCTCAC 
TGCGTGCTTT TCTATACCTA TCATGACGTG ACCAGACTGA AGAGACTTAT AATGATGGAA 
AAAGATGGAA ACTATCATAC AAGGGAAACT CACGTCAATA ATTTATATAG CATGGTACAT 
TACTGCGAAA ACATAACAGA ATGCAGAAGA ATACAGCTTT TAGCTTACTT TGGTGAAAAA 
GGATTTAACC CTGATTTTTG TAAGAAACAT CCAGATGTTT CTTGTGACAA TTGCTGTAAA 
ACAAAGGATT ATAAAACAAA AGATGTGACT GATGATGTGA AAAATATTGT AAGATTTGTT 
CAAGAACACA GTTCATCACC AGGAACAAGA AATATAGGAC CTGCTGGAAG ATTTACTCTG 
AACATGCTAG TCGACATTTT CTTGGGAAGT AAGAGTGCAA AAGTTCAGTC TGGAATATTT 
GGAAAGGGGT CTGCATATTC ACGCCACAAT GCTGAAAGAC TTTTTAAAAA GCTGATTCTA 
GACAAAATCT TGGATGAAGA CTTATATATC AATGCCAATG ACCAACCAAT TGCCTATGTG 
ATGCTCGGAA CAAAAGCCCA CAGTGTGCTG AGCGGCCACT TGAAGGTGGA CTTCATGGAA 
ACGGAAAATT CGAGCAGTGT TAAAAAACAA AAAGCCGCAG TGGCCCACGT GTCCCAGAGA 
GACGAGGCAG TTATGAAGTG TCTGGGAGAA CTTGCAGACG TCTGCAAATT GCTGGGCAAA 
GTCTTTGGTG TCCATTACTT CAATATTTTC AATACAGCCA CCCTCAAAAA GCTTGCAGAA 
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAGATTGATG GTGTTACTGA AGACAAGCTG 
GAGAAATATG GTGCAGAAAT AATTCCAGTA TTACAGAAGT ACGCAGAGTG GACATTGCCA 
GCTGAAGGTG GGTCCCCAGG CAGCAGAGGC TCTAGGAGGA GCACCCCAGA GGAGACGGAG 
GAGGAGGAAG CTGTATCTTC TCACTACTTT GCAAATCAAA CTAGAAATGA AAGAAAGAGG 
AAGAAAGTGT CAGCCTCCCA AAAGCCCAAG AGAAGAAGAA CTAGTTACGG CGGCGGCTTC 
AGAGCAAAGG GGGGCTCTAC TACATGCAGA AAACCATCTT CTAAAAGTAA ATTCTCTGGT 
GTAACTGGAT CCCGCTCAGC CTCGTGTGCT TCTCAGGCAA CATCAGCAGC CAGTAGAAAA 
CTGGGGATTA TGGCTCCTCC AAAGCCTGTA AATAGACCGT TCCTCAGGCC TGCGTATGCC 
TTCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blm ( XM_017589087.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444576.1 Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa53705
Clone ID Related Accession (Same CDS sequence)	XM_017589087.1
Accession Version	XM_017589087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Bloom syndrome protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGAAG AAGTTGACAG TGCAAAGAAG AGCCATGAAG ACGAGGCTGC ATTCCAGTCA 
CCTCAGAACG CTCTGTACTT TGAACACAAT GACAATGATT ATGATATAGA TTTTGTTCCA 
CCTTCTCCAG AAGAAATCAT TTCCACTGCT TCTTCCTCGT TGAAATGTTC CAGTATGCTA 
AAGGATCTCG ATGACTCTGA CAAAGAGAAG GACATTCTGA GTACCTCAAA AGATCTGTCA 
AAACCAGAGG AAATGACCAC ACCGAAGTCT GATCTGGGAA CCAATGAAGA CTGTGATGCC 
CAGCAGACTA GCTTGCAGCA GCAGCTTATC CGTGTGATGG AGCACATCTG TAAATTAGTT 
GATACTGTTC CTATTGATGA ACTGAAAGCT CTGACTTGTG GGGACAAACT GCTTCAGCAG 
CGAGACATAA GAAGGAAGCT CCTTGCTGAA GCAGGTTTTA ATAGAAACGA TGTCAATCTT 
CTGGGTTCTT TGTGGAGGCA CAGGCCAGAT TCGCTTGATA ACACTGTGCA GTGTGACTCC 
TGTCCTGTGG GGCATCCTGG TAAAGAGTTA AACTCTCCAT ACCTTCCCTC ACACTCCCTC 
TCCACTGAGG AATGCTTACC CACCACTGCT TCAGGAAAGA CAGGATTCTC AGCCACCCCG 
AAGAGTCTCT CTGAAAGGCC ACCATTGAAT TCCCACTTAC AGAAGTCCTT TGTAAGTAGC 
AACTGGGCTG AAACACCAAG GACAGAAAGC AGGACGGAAA GCACTTACTT CCCAGGGAGT 
GTTCTCACAA GCACCACTGT GAAGGATGAG AGTAAACATG TTGCTTCAGG ACGTGACATA 
GAGAGAGAAG TCCAGGCTTC CTACGATATT GATAATTTTA ACATTGATGA CTTTGATGAT 
GATGATGATG ATGACTGGGA AAACATAATG CACAATTTTT CAGTCAGCAA ATCTTCCACA 
GCTGCCTACC CACCTATCAA GGAAGGTGGC CCAGTTAAAT CTCTATCAGA AAGGATTTCT 
TCAGCCAAGG CAAAGTTTCT TCCAGTGGCA TCAACCGCTC AAAATAAAAA CCTCTCAGAG 
TCAATTCAGA ATTGCTCTGA TAAGTTTGCA CAAAATCTAT CATCAAAAAA TCCAAAACAT 
GAACATTTTC AAAGTCTTAA TTTTCCTCAT ACAAAAGAAA TGATGAAGAT TTTCCGTAAG 
AAATTTGGCT TGCATAATTT TAGAACTAAT CAGCTAGAGG CGATCAATGC TGCGCTGCTT 
GGTGAAGACT GCTTTATCCT GATGCCCACT GGAGGTGGTA AAAGTTTGTG CTACCAGCTC 
CCTGCCTGTG TTTCTCCTGG AGTCACTATT GTCATTTCTC CCTTGAGATC ACTAATAGTG 
GACCAAGTCC AAAAGCTGAC TTCCTTTGAT ATTCCAGCTA CGTACCTGAC AGGGGATAAG 
ACTGACTCCG AAGCTGCAAA CATTTACCTC CAGTTGTCAA AGAAAGACCC GATCATCAAG 
CTTCTGTATG TCACCCCAGA GAAGGTCTGT GCAAGTAACA GGCTGATTTC CACTCTGGAG 
AATCTGTATG AGCGGAAGCT CTTGGCACGA TTTGTCATTG ATGAAGCACA CTGTGTGAGT 
CAGTGGGGCC ATGATTTCCG TCAAGATTAC AAAAGGATGA ATATGCTTCG CCAGAGGTTC 
CCTTCTGTCC CAGTGATGGC TCTCACGGCC ACAGCGAACC CCAGGGTACA GAAGGACATC 
CTCACGCAGC TGAAGATTCT CAGGCCTCAG GTGTTTAGCA TGAGCTTTAA CAGACACAAT 
CTGAAATACT ATGTATTACC AAAGAAGCCC AAGAAAGTGG CACTTGATTG CCTAGAGTGG 
ATCAGAAAGC ATCACCCTTA TGACTCGGGG ATAATTTATT GCCTCTCCAG GAAGGAATGT 
GACACAATGG CCGACACCTT ACAGAGAGAA GGTCTTGCTG CTCTGGCTTA CCATGCAGGT 
CTTAGTGATT CTGCCAGAGA TGAGGTGCAG CACAAGTGGA TCAACCAGGA CAACTGCCAG 
GTTATCTGTG CAACCATTGC ATTTGGAATG GGAATCGACA AACCTGACGT GCGATTTGTG 
GTTCATGCAT CTCTTCCTAA ATCTGTGGAG GGTTATTACC AAGAATCCGG CCGAGCTGGG 
AGAGACGGGG AAATATCTCA CTGCGTGCTT TTCTATACCT ATCATGACGT GACCAGACTG 
AAGAGACTTA TAATGATGGA AAAAGATGGA AACTATCATA CAAGGGAAAC TCACGTCAAT 
AATTTATATA GCATGGTACA TTACTGCGAA AACATAACAG AATGCAGAAG AATACAGCTT 
TTAGCTTACT TTGGTGAAAA AGGATTTAAC CCTGATTTTT GTAAGAAACA TCCAGATGTT 
TCTTGTGACA ATTGCTGTAA AACAAAGGAT TATAAAACAA AAGATGTGAC TGATGATGTG 
AAAAATATTG TAAGATTTGT TCAAGAACAC AGTTCATCAC CAGGAACAAG AAATATAGGA 
CCTGCTGGAA GATTTACTCT GAACATGCTA GTCGACATTT TCTTGGGAAG TAAGAGTGCA 
AAAGTTCAGT CTGGAATATT TGGAAAGGGG TCTGCATATT CACGCCACAA TGCTGAAAGA 
CTTTTTAAAA AGCTGATTCT AGACAAAATC TTGGATGAAG ACTTATATAT CAATGCCAAT 
GACCAACCAA TTGCCTATGT GATGCTCGGA ACAAAAGCCC ACAGTGTGCT GAGCGGCCAC 
TTGAAGGTGG ACTTCATGGA AACGGAAAAT TCGAGCAGTG TTAAAAAACA AAAAGCCGCA 
GTGGCCCACG TGTCCCAGAG AGACGAGGCA GTTATGAAGT GTCTGGGAGA ACTTGCAGAC 
GTCTGCAAAT TGCTGGGCAA AGTCTTTGGT GTCCATTACT TCAATATTTT CAATACAGCC 
ACCCTCAAAA AGCTTGCAGA ATCTTTATCT TCTGATCCTG AGGTTTTGCT TCAGATTGAT 
GGTGTTACTG AAGACAAGCT GGAGAAATAT GGTGCAGAAA TAATTCCAGT ATTACAGAAG 
TACGCAGAGT GGACATTGCC AGCTGAAGGT GGGTCCCCAG GCAGCAGAGG CTCTAGGAGG 
AGCACCCCAG AGGAGACGGA GGAGGAGGAA GCTGTATCTT CTCACTACTT TGCAAATCAA 
ACTAGAAATG AAAGAAAGAG GAAGAAAGTG TCAGCCTCCC AAAAGCCCAA GAGAAGAAGA 
ACTAGTTACG GCGGCGGCTT CAGAGCAAAG GGGGGCTCTA CTACATGCAG AAAACCATCT 
TCTAAAAGTA AATTCTCTGG TGTAACTGGA TCCCGCTCAG CCTCGTGTGC TTCTCAGGCA 
ACATCAGCAG CCAGTAGAAA ACTGGGGATT ATGGCTCCTC CAAAGCCTGT AAATAGACCG 
TTCCTCAGGC CTGCGTATGC CTTCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444576.1
CDS	124..3573
Translation	MHEEVDSAKKSHEDEAAFQSPQNALYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKDILSTSKDLSKPEEMTTPKSDLGTNEDCDAQQTSLQQQLIRVMEHICKLVDTVPIDELKALTCGDKLLQQRDIRRKLLAEAGFNRNDVNLLGSLWRHRPDSLDNTVQCDSCPVGHPGKELNSPYLPSHSLSTEECLPTTASGKTGFSATPKSLSERPPLNSHLQKSFVSSNWAETPRTESRTESTYFPGSVLTSTTVKDESKHVASGRDIEREVQASYDIDNFNIDDFDDDDDDDWENIMHNFSVSKSSTAAYPPIKEGGPVKSLSERISSAKAKFLPVASTAQNKNLSESIQNCSDKFAQNLSSKNPKHEHFQSLNFPHTKEMMKIFRKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQRFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVALDCLEWIRKHHPYDSGIIYCLSRKECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVVHASLPKSVEGYYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTRETHVNNLYSMVHYCENITECRRIQLLAYFGEKGFNPDFCKKHPDVSCDNCCKTKDYKTKDVTDDVKNIVRFVQEHSSSPGTRNIGPAGRFTLNMLVDIFLGSKSAKVQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHLKVDFMETENSSSVKKQKAAVAHVSQRDEAVMKCLGELADVCKLLGKVFGVHYFNIFNTATLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEIIPVLQKYAEWTLPAEGGSPGSRGSRRSTPEETEEEEAVSSHYFANQTRNERKRKKVSASQKPKRRRTSYGGGFRAKGGSTTCRKPSSKSKFSGVTGSRSASCASQATSAASRKLGIMAPPKPVNRPFLRPAYAFS

Target ORF information:

RefSeq Version	XM_017589087.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589087.1

ORF Insert Sequence:

ATGCATGAAG AAGTTGACAG TGCAAAGAAG AGCCATGAAG ACGAGGCTGC ATTCCAGTCA 
CCTCAGAACG CTCTGTACTT TGAACACAAT GACAATGATT ATGATATAGA TTTTGTTCCA 
CCTTCTCCAG AAGAAATCAT TTCCACTGCT TCTTCCTCGT TGAAATGTTC CAGTATGCTA 
AAGGATCTCG ATGACTCTGA CAAAGAGAAG GACATTCTGA GTACCTCAAA AGATCTGTCA 
AAACCAGAGG AAATGACCAC ACCGAAGTCT GATCTGGGAA CCAATGAAGA CTGTGATGCC 
CAGCAGACTA GCTTGCAGCA GCAGCTTATC CGTGTGATGG AGCACATCTG TAAATTAGTT 
GATACTGTTC CTATTGATGA ACTGAAAGCT CTGACTTGTG GGGACAAACT GCTTCAGCAG 
CGAGACATAA GAAGGAAGCT CCTTGCTGAA GCAGGTTTTA ATAGAAACGA TGTCAATCTT 
CTGGGTTCTT TGTGGAGGCA CAGGCCAGAT TCGCTTGATA ACACTGTGCA GTGTGACTCC 
TGTCCTGTGG GGCATCCTGG TAAAGAGTTA AACTCTCCAT ACCTTCCCTC ACACTCCCTC 
TCCACTGAGG AATGCTTACC CACCACTGCT TCAGGAAAGA CAGGATTCTC AGCCACCCCG 
AAGAGTCTCT CTGAAAGGCC ACCATTGAAT TCCCACTTAC AGAAGTCCTT TGTAAGTAGC 
AACTGGGCTG AAACACCAAG GACAGAAAGC AGGACGGAAA GCACTTACTT CCCAGGGAGT 
GTTCTCACAA GCACCACTGT GAAGGATGAG AGTAAACATG TTGCTTCAGG ACGTGACATA 
GAGAGAGAAG TCCAGGCTTC CTACGATATT GATAATTTTA ACATTGATGA CTTTGATGAT 
GATGATGATG ATGACTGGGA AAACATAATG CACAATTTTT CAGTCAGCAA ATCTTCCACA 
GCTGCCTACC CACCTATCAA GGAAGGTGGC CCAGTTAAAT CTCTATCAGA AAGGATTTCT 
TCAGCCAAGG CAAAGTTTCT TCCAGTGGCA TCAACCGCTC AAAATAAAAA CCTCTCAGAG 
TCAATTCAGA ATTGCTCTGA TAAGTTTGCA CAAAATCTAT CATCAAAAAA TCCAAAACAT 
GAACATTTTC AAAGTCTTAA TTTTCCTCAT ACAAAAGAAA TGATGAAGAT TTTCCGTAAG 
AAATTTGGCT TGCATAATTT TAGAACTAAT CAGCTAGAGG CGATCAATGC TGCGCTGCTT 
GGTGAAGACT GCTTTATCCT GATGCCCACT GGAGGTGGTA AAAGTTTGTG CTACCAGCTC 
CCTGCCTGTG TTTCTCCTGG AGTCACTATT GTCATTTCTC CCTTGAGATC ACTAATAGTG 
GACCAAGTCC AAAAGCTGAC TTCCTTTGAT ATTCCAGCTA CGTACCTGAC AGGGGATAAG 
ACTGACTCCG AAGCTGCAAA CATTTACCTC CAGTTGTCAA AGAAAGACCC GATCATCAAG 
CTTCTGTATG TCACCCCAGA GAAGGTCTGT GCAAGTAACA GGCTGATTTC CACTCTGGAG 
AATCTGTATG AGCGGAAGCT CTTGGCACGA TTTGTCATTG ATGAAGCACA CTGTGTGAGT 
CAGTGGGGCC ATGATTTCCG TCAAGATTAC AAAAGGATGA ATATGCTTCG CCAGAGGTTC 
CCTTCTGTCC CAGTGATGGC TCTCACGGCC ACAGCGAACC CCAGGGTACA GAAGGACATC 
CTCACGCAGC TGAAGATTCT CAGGCCTCAG GTGTTTAGCA TGAGCTTTAA CAGACACAAT 
CTGAAATACT ATGTATTACC AAAGAAGCCC AAGAAAGTGG CACTTGATTG CCTAGAGTGG 
ATCAGAAAGC ATCACCCTTA TGACTCGGGG ATAATTTATT GCCTCTCCAG GAAGGAATGT 
GACACAATGG CCGACACCTT ACAGAGAGAA GGTCTTGCTG CTCTGGCTTA CCATGCAGGT 
CTTAGTGATT CTGCCAGAGA TGAGGTGCAG CACAAGTGGA TCAACCAGGA CAACTGCCAG 
GTTATCTGTG CAACCATTGC ATTTGGAATG GGAATCGACA AACCTGACGT GCGATTTGTG 
GTTCATGCAT CTCTTCCTAA ATCTGTGGAG GGTTATTACC AAGAATCCGG CCGAGCTGGG 
AGAGACGGGG AAATATCTCA CTGCGTGCTT TTCTATACCT ATCATGACGT GACCAGACTG 
AAGAGACTTA TAATGATGGA AAAAGATGGA AACTATCATA CAAGGGAAAC TCACGTCAAT 
AATTTATATA GCATGGTACA TTACTGCGAA AACATAACAG AATGCAGAAG AATACAGCTT 
TTAGCTTACT TTGGTGAAAA AGGATTTAAC CCTGATTTTT GTAAGAAACA TCCAGATGTT 
TCTTGTGACA ATTGCTGTAA AACAAAGGAT TATAAAACAA AAGATGTGAC TGATGATGTG 
AAAAATATTG TAAGATTTGT TCAAGAACAC AGTTCATCAC CAGGAACAAG AAATATAGGA 
CCTGCTGGAA GATTTACTCT GAACATGCTA GTCGACATTT TCTTGGGAAG TAAGAGTGCA 
AAAGTTCAGT CTGGAATATT TGGAAAGGGG TCTGCATATT CACGCCACAA TGCTGAAAGA 
CTTTTTAAAA AGCTGATTCT AGACAAAATC TTGGATGAAG ACTTATATAT CAATGCCAAT 
GACCAACCAA TTGCCTATGT GATGCTCGGA ACAAAAGCCC ACAGTGTGCT GAGCGGCCAC 
TTGAAGGTGG ACTTCATGGA AACGGAAAAT TCGAGCAGTG TTAAAAAACA AAAAGCCGCA 
GTGGCCCACG TGTCCCAGAG AGACGAGGCA GTTATGAAGT GTCTGGGAGA ACTTGCAGAC 
GTCTGCAAAT TGCTGGGCAA AGTCTTTGGT GTCCATTACT TCAATATTTT CAATACAGCC 
ACCCTCAAAA AGCTTGCAGA ATCTTTATCT TCTGATCCTG AGGTTTTGCT TCAGATTGAT 
GGTGTTACTG AAGACAAGCT GGAGAAATAT GGTGCAGAAA TAATTCCAGT ATTACAGAAG 
TACGCAGAGT GGACATTGCC AGCTGAAGGT GGGTCCCCAG GCAGCAGAGG CTCTAGGAGG 
AGCACCCCAG AGGAGACGGA GGAGGAGGAA GCTGTATCTT CTCACTACTT TGCAAATCAA 
ACTAGAAATG AAAGAAAGAG GAAGAAAGTG TCAGCCTCCC AAAAGCCCAA GAGAAGAAGA 
ACTAGTTACG GCGGCGGCTT CAGAGCAAAG GGGGGCTCTA CTACATGCAG AAAACCATCT 
TCTAAAAGTA AATTCTCTGG TGTAACTGGA TCCCGCTCAG CCTCGTGTGC TTCTCAGGCA 
ACATCAGCAG CCAGTAGAAA ACTGGGGATT ATGGCTCCTC CAAAGCCTGT AAATAGACCG 
TTCCTCAGGC CTGCGTATGC CTTCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blm ( XM_017590165.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445654.1 Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X3, mRNA.

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CloneID	ORa56975
Clone ID Related Accession (Same CDS sequence)	XM_017590165.1
Accession Version	XM_017590165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Bloom syndrome protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG TTCCTCTGAA CAATCTACAA GAACAACTAC AGCGTCACTC GGCCAGAAAA 
CTTAAAAATC AGTTCAGCAT TTCCAAACCA AAACCTTCAG GTTTTACTTT TAAAAAGAAA 
ACACCAGAGG GTGATGTGTC TGTGGTAAGA ACACCTGTGT TAAGCGATAA AGATGTGAAT 
ATGTCTGAGG CCTTGTCATA TACTGAGCCT CCACTCCACA AACCAAAGCA GCAGTCAAAC 
ATCGATGGCT TCTTTAAAAA TTCCCCTGGA GGGCAGCAAA GCAAGGAGAC CTGCTCCGAA 
CTGTCCCTGC CGGAGGTGGT ACAGATTCCT CAGGACGCTT CTTGTACTAC CCCAAAAACC 
CCAGCTGTGA AGAAACCCCA CGTGGCCATT TTCAAAAAAC TGGAATTTAG TTCTTCGGCA 
GACTCTCTCA GTGATTGGGC TGATATAGAT GACTTTGATG TGCCAGCATC AGACGCATTT 
GTTTCCCTGA CCAGAAACCC TACTACAAGA GTGAGCACTG CTAAGAAACT GAGTAGCAGT 
AAGAGAAACT TCTTTAAACC ACCACCTAGA GCAAGTGCAG TAAAGGCCGA CTTGACTCCT 
TCCTCCCCCG AATGCCTGCA AGTGGATTTA ACGAAGGAAC AGGAGGAAGA GGCGGACTGC 
CTGAGCAGGG ATGTGATCTG CATTGACACT GACCCTGCTT CTGAGGAGCT CTTGGAGAAG 
GATGTGCAGG AAGGCCTGTC ATTGGACGCT CGCTTGGGGA CTGAAAGAGG TATTGACAGT 
GCAAAGAAGA GCCATGAAGA CAAGGCTGCA TTCCAGTCAC CTCAGAACGC TCTGTACTTT 
GAACACAATG ACAATGATTA TGATATAGAT TTTGTTCCAC CTTCTCCAGA AGAAATCATT 
TCCACTGCTT CTTCCTCGTT GAAATGTTCC AGTATGCTAA AGGATCTCGA TGACTCTGAC 
AAAGAGAAGG ACATTCTGAG TACCTCAAAA GATCTGTCAA AACCAGAGGA AATGACCACA 
CCGAAGTCTG ATCTGGGAAC CAATGAAGAC TGTGATGCCC AGCAGACTAG CTTGCAGCAG 
CAGCTTATCC GTGTGATGGA GCACATCTGT AAATTAGTTG ATACTGTTCC TATTGATGAA 
CTGAAAGCTC TGACTTGTGG GGACAAACTG CTTCAGCAGC GAGACATAAG AAGGAAGCTC 
CTTGCTGAAG CAGGTTTTAA TAGAAACGAT GTCAATCTTC TGGGTTCTTT GTGGAGGCAC 
AGGCCAGATT CGCTTGATAA CACTGTGCAG TGTGACTCCT GTCCTGTGGG GCATCCTGGT 
AAAGAGTTAA ACTCTCCATA CCTTCCCTCA CACTCCCTCT CCACTGAGGA ATGCTTACCC 
ACCACTGCTT CAGGAAAGAC AGGATTCTCA GCCACCCCGA AGAGTCTCTC TGAAAGGCCA 
CCATTGAATT CCCACTTACA GAAGTCCTTT GTAAGTAGCA ACTGGGCTGA AACACCAAGG 
ACAGAAAGCA GGACGGAAAG CACTTACTTC CCAGGGAGTG TTCTCACAAG CACCACTGTG 
AAGGATGAGA GTAAACATGT TGCTTCAGGA CGTGACATAG AGAGAGAAGT CCAGGCTTCC 
TACGATATTG ATAATTTTAA CATTGATGAC TTTGATGATG ATGATGATGA TGACTGGGAA 
AACATAATGC ACAATTTTTC AGTCAGCAAA TCTTCCACAG CTGCCTACCC ACCTATCAAG 
GAAGGTGGCC CAGTTAAATC TCTATCAGAA AGGATTTCTT CAGCCAAGGC AAAGTTTCTT 
CCAGTGGCAT CAACCGCTCA AAATAAAAAC CTCTCAGAGT CAATTCAGAA TTGCTCTGAT 
AAGTTTGCAC AAAATCTATC ATCAAAAAAT CCAAAACATG AACATTTTCA AAGTCTTAAT 
TTTCCTCATA CAAAAGAAAT GATGAAGATT TTCCGTAAGA AATTTGGCTT GCATAATTTT 
AGAACTAATC AGCTAGAGGC GATCAATGCT GCGCTGCTTG GTGAAGACTG CTTTATCCTG 
ATGCCCACTG GAGGTGGTAA AAGTTTGTGC TACCAGCTCC CTGCCTGTGT TTCTCCTGGA 
GTCACTATTG TCATTTCTCC CTTGAGATCA CTAATAGTGG ACCAAGTCCA AAAGCTGACT 
TCCTTTGATA TTCCAGCTAC GTACCTGACA GGGGATAAGA CTGACTCCGA AGCTGCAAAC 
ATTTACCTCC AGTTGTCAAA GAAAGACCCG ATCATCAAGC TTCTGTATGT CACCCCAGAG 
AAGGTCTGTG CAAGTAACAG GCTGATTTCC ACTCTGGAGA ATCTGTATGA GCGAAAGCTC 
TTGGCACGAT TTGTCATTGA TGAAGCACAC TGTGTGAGTC AGTGGGGCCA TGATTTCCGT 
CAAGATTACA AAAGGATGAA TATGCTTCGC CAGAGGTTCC CTTCTGTCCC AGTGATGGCT 
CTCACGGCCA CAGCGAACCC CAGGGTACAG AAGGACATCC TCACGCAGCT GAAGATTCTC 
AGGCCTCAGG TGTTTAGCAT GAGCTTTAAC AGACACAATC TGAAATACTA TGTATTACCA 
AAGAAGCCCA AGAAAGTGGC ACTTGATTGC CTAGAGTGGA TCAGAAAGCA TCACCCTTAT 
GACTCGGGGA TAATTTATTG CCTCTCCAGG AAGGAATGTG ACACAATGGC CGACACCTTA 
CAGAGAGAAG GTCTTGCTGC TCTGGCTTAC CATGCAGGTC TTAGTGATTC TGCCAGAGAT 
GAGGTGCAGC ACAAGTGGAT CAACCAGGAC AACTGCCAGG TTATCTGTGC AACCATTGCA 
TTTGGAATGG GAATCGACAA ACCTGACGTG CGATTTGTGG TTCATGCATC TCTTCCTAAA 
TCTGTGGAGG GTTATTACCA AGAATCCGGC CGAGCTGGGA GAGACGGGGA AATATCTCAC 
TGCGTGCTTT TCTATACCTA TCATGACGTG ACCAGACTGA AGAGACTTAT AATGATGGAA 
AAAGATGGAA ACTATCATAC AAGGGAAACT CACGTCAATA ATTTATATAG CATGGTACAT 
TACTGCGAAA ACATAACAGA ATGCAGAAGA ATACAGCTTT TAGCTTACTT TGGTGAAAAA 
GGATTTAACC CTGATTTTTG TAAGAAACAT CCAGATGTTT CTTGTGACAA TTGCTGTAAA 
ACAAAGGATT ATAAAACAAA AGATGTGACT GATGATGTGA AAAATATTGT AAGATTTGTT 
CAAGAACACA GTTCATCACC AGGAACAAGA AATATAGGAC CTGCTGGAAG ATTTACTCTG 
AACATGCTAG TCGACATTTT CTTGGGAAGT AAGAGTGCAA AAGTTCAGTC TGGAATATTT 
GGAAAGGGGT CTGCATATTC ACGCCACAAT GCTGAAAGAC TTTTTAAAAA GCTGATTCTA 
GACAAAATCT TGGATGAAGA CTTATATATC AATGCCAATG ACCAACCAAT TGCCTATGTG 
ATGCTCGGAA CAAAAGCCCA CAGTGTGCTG AGCGGCCACT TGAAGGTGGA CTTCATGGAA 
ACGGAAAATT CGAGCAGTGT TAAAAAACAA AAAGCCGCAG TGGCCCACGT GTCCCAGAGA 
GACGAGGCAG TTATGAAGTG TCTGGGAGAA CTTGCAGACG TCTGCAAATT GCTGGGCAAA 
GTCTTTGGTG TCCATTACTT CAATATTTTC AATACAGCCA CCCTCAAAAA GCTTGCAGAA 
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAGATTGATG GTGTTACTGA AGACAAGCTG 
GAGAAATATG GTGCAGAAAT AATTCCAGTA TTACAGAAGT ACGCAGAGTG GACATTGCCA 
GCTGAAGGTG GGTCCCCAGG CAGCAGAGGC TCTAGGAGGA GCACCCCAGA GGAGACGGAG 
GAGGAGGAAG CTGTATCTTC TCACTACTTT GCAAATCAAA CTAGAAATGA AAGAAAGAGG 
AAGAAAGTGT CAGCCTCCCA AAAGCCCAAG AGAAGAAGAA CTAGTTACGG CGGCGGCTTC 
AGAGCAAAGG GGGGCTCTAC TACATGCAGA AAACCATCTT CTAAAAGTAA ATTCTCTGGT 
GTAACTGGAT CCCGCTCAGC CTCGTGTGCT TCTCAGGCAA CATCAGCAGC CAGTAGAAAA 
CTGGGGATTA TGGCTCCTCC AAAGCCTGTA AATAGACCGT TCCTCAGGCC TGCGTATGCC 
TTCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445654.1
CDS	123..4331
Translation	MAAVPLNNLQEQLQRHSARKLKNQFSISKPKPSGFTFKKKTPEGDVSVVRTPVLSDKDVNMSEALSYTEPPLHKPKQQSNIDGFFKNSPGGQQSKETCSELSLPEVVQIPQDASCTTPKTPAVKKPHVAIFKKLEFSSSADSLSDWADIDDFDVPASDAFVSLTRNPTTRVSTAKKLSSSKRNFFKPPPRASAVKADLTPSSPECLQVDLTKEQEEEADCLSRDVICIDTDPASEELLEKDVQEGLSLDARLGTERGIDSAKKSHEDKAAFQSPQNALYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKDILSTSKDLSKPEEMTTPKSDLGTNEDCDAQQTSLQQQLIRVMEHICKLVDTVPIDELKALTCGDKLLQQRDIRRKLLAEAGFNRNDVNLLGSLWRHRPDSLDNTVQCDSCPVGHPGKELNSPYLPSHSLSTEECLPTTASGKTGFSATPKSLSERPPLNSHLQKSFVSSNWAETPRTESRTESTYFPGSVLTSTTVKDESKHVASGRDIEREVQASYDIDNFNIDDFDDDDDDDWENIMHNFSVSKSSTAAYPPIKEGGPVKSLSERISSAKAKFLPVASTAQNKNLSESIQNCSDKFAQNLSSKNPKHEHFQSLNFPHTKEMMKIFRKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQRFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVALDCLEWIRKHHPYDSGIIYCLSRKECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVVHASLPKSVEGYYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTRETHVNNLYSMVHYCENITECRRIQLLAYFGEKGFNPDFCKKHPDVSCDNCCKTKDYKTKDVTDDVKNIVRFVQEHSSSPGTRNIGPAGRFTLNMLVDIFLGSKSAKVQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHLKVDFMETENSSSVKKQKAAVAHVSQRDEAVMKCLGELADVCKLLGKVFGVHYFNIFNTATLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEIIPVLQKYAEWTLPAEGGSPGSRGSRRSTPEETEEEEAVSSHYFANQTRNERKRKKVSASQKPKRRRTSYGGGFRAKGGSTTCRKPSSKSKFSGVTGSRSASCASQATSAASRKLGIMAPPKPVNRPFLRPAYAFS

Target ORF information:

RefSeq Version	XM_017590165.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590165.1

ORF Insert Sequence:

ATGGCTGCTG TTCCTCTGAA CAATCTACAA GAACAACTAC AGCGTCACTC GGCCAGAAAA 
CTTAAAAATC AGTTCAGCAT TTCCAAACCA AAACCTTCAG GTTTTACTTT TAAAAAGAAA 
ACACCAGAGG GTGATGTGTC TGTGGTAAGA ACACCTGTGT TAAGCGATAA AGATGTGAAT 
ATGTCTGAGG CCTTGTCATA TACTGAGCCT CCACTCCACA AACCAAAGCA GCAGTCAAAC 
ATCGATGGCT TCTTTAAAAA TTCCCCTGGA GGGCAGCAAA GCAAGGAGAC CTGCTCCGAA 
CTGTCCCTGC CGGAGGTGGT ACAGATTCCT CAGGACGCTT CTTGTACTAC CCCAAAAACC 
CCAGCTGTGA AGAAACCCCA CGTGGCCATT TTCAAAAAAC TGGAATTTAG TTCTTCGGCA 
GACTCTCTCA GTGATTGGGC TGATATAGAT GACTTTGATG TGCCAGCATC AGACGCATTT 
GTTTCCCTGA CCAGAAACCC TACTACAAGA GTGAGCACTG CTAAGAAACT GAGTAGCAGT 
AAGAGAAACT TCTTTAAACC ACCACCTAGA GCAAGTGCAG TAAAGGCCGA CTTGACTCCT 
TCCTCCCCCG AATGCCTGCA AGTGGATTTA ACGAAGGAAC AGGAGGAAGA GGCGGACTGC 
CTGAGCAGGG ATGTGATCTG CATTGACACT GACCCTGCTT CTGAGGAGCT CTTGGAGAAG 
GATGTGCAGG AAGGCCTGTC ATTGGACGCT CGCTTGGGGA CTGAAAGAGG TATTGACAGT 
GCAAAGAAGA GCCATGAAGA CAAGGCTGCA TTCCAGTCAC CTCAGAACGC TCTGTACTTT 
GAACACAATG ACAATGATTA TGATATAGAT TTTGTTCCAC CTTCTCCAGA AGAAATCATT 
TCCACTGCTT CTTCCTCGTT GAAATGTTCC AGTATGCTAA AGGATCTCGA TGACTCTGAC 
AAAGAGAAGG ACATTCTGAG TACCTCAAAA GATCTGTCAA AACCAGAGGA AATGACCACA 
CCGAAGTCTG ATCTGGGAAC CAATGAAGAC TGTGATGCCC AGCAGACTAG CTTGCAGCAG 
CAGCTTATCC GTGTGATGGA GCACATCTGT AAATTAGTTG ATACTGTTCC TATTGATGAA 
CTGAAAGCTC TGACTTGTGG GGACAAACTG CTTCAGCAGC GAGACATAAG AAGGAAGCTC 
CTTGCTGAAG CAGGTTTTAA TAGAAACGAT GTCAATCTTC TGGGTTCTTT GTGGAGGCAC 
AGGCCAGATT CGCTTGATAA CACTGTGCAG TGTGACTCCT GTCCTGTGGG GCATCCTGGT 
AAAGAGTTAA ACTCTCCATA CCTTCCCTCA CACTCCCTCT CCACTGAGGA ATGCTTACCC 
ACCACTGCTT CAGGAAAGAC AGGATTCTCA GCCACCCCGA AGAGTCTCTC TGAAAGGCCA 
CCATTGAATT CCCACTTACA GAAGTCCTTT GTAAGTAGCA ACTGGGCTGA AACACCAAGG 
ACAGAAAGCA GGACGGAAAG CACTTACTTC CCAGGGAGTG TTCTCACAAG CACCACTGTG 
AAGGATGAGA GTAAACATGT TGCTTCAGGA CGTGACATAG AGAGAGAAGT CCAGGCTTCC 
TACGATATTG ATAATTTTAA CATTGATGAC TTTGATGATG ATGATGATGA TGACTGGGAA 
AACATAATGC ACAATTTTTC AGTCAGCAAA TCTTCCACAG CTGCCTACCC ACCTATCAAG 
GAAGGTGGCC CAGTTAAATC TCTATCAGAA AGGATTTCTT CAGCCAAGGC AAAGTTTCTT 
CCAGTGGCAT CAACCGCTCA AAATAAAAAC CTCTCAGAGT CAATTCAGAA TTGCTCTGAT 
AAGTTTGCAC AAAATCTATC ATCAAAAAAT CCAAAACATG AACATTTTCA AAGTCTTAAT 
TTTCCTCATA CAAAAGAAAT GATGAAGATT TTCCGTAAGA AATTTGGCTT GCATAATTTT 
AGAACTAATC AGCTAGAGGC GATCAATGCT GCGCTGCTTG GTGAAGACTG CTTTATCCTG 
ATGCCCACTG GAGGTGGTAA AAGTTTGTGC TACCAGCTCC CTGCCTGTGT TTCTCCTGGA 
GTCACTATTG TCATTTCTCC CTTGAGATCA CTAATAGTGG ACCAAGTCCA AAAGCTGACT 
TCCTTTGATA TTCCAGCTAC GTACCTGACA GGGGATAAGA CTGACTCCGA AGCTGCAAAC 
ATTTACCTCC AGTTGTCAAA GAAAGACCCG ATCATCAAGC TTCTGTATGT CACCCCAGAG 
AAGGTCTGTG CAAGTAACAG GCTGATTTCC ACTCTGGAGA ATCTGTATGA GCGAAAGCTC 
TTGGCACGAT TTGTCATTGA TGAAGCACAC TGTGTGAGTC AGTGGGGCCA TGATTTCCGT 
CAAGATTACA AAAGGATGAA TATGCTTCGC CAGAGGTTCC CTTCTGTCCC AGTGATGGCT 
CTCACGGCCA CAGCGAACCC CAGGGTACAG AAGGACATCC TCACGCAGCT GAAGATTCTC 
AGGCCTCAGG TGTTTAGCAT GAGCTTTAAC AGACACAATC TGAAATACTA TGTATTACCA 
AAGAAGCCCA AGAAAGTGGC ACTTGATTGC CTAGAGTGGA TCAGAAAGCA TCACCCTTAT 
GACTCGGGGA TAATTTATTG CCTCTCCAGG AAGGAATGTG ACACAATGGC CGACACCTTA 
CAGAGAGAAG GTCTTGCTGC TCTGGCTTAC CATGCAGGTC TTAGTGATTC TGCCAGAGAT 
GAGGTGCAGC ACAAGTGGAT CAACCAGGAC AACTGCCAGG TTATCTGTGC AACCATTGCA 
TTTGGAATGG GAATCGACAA ACCTGACGTG CGATTTGTGG TTCATGCATC TCTTCCTAAA 
TCTGTGGAGG GTTATTACCA AGAATCCGGC CGAGCTGGGA GAGACGGGGA AATATCTCAC 
TGCGTGCTTT TCTATACCTA TCATGACGTG ACCAGACTGA AGAGACTTAT AATGATGGAA 
AAAGATGGAA ACTATCATAC AAGGGAAACT CACGTCAATA ATTTATATAG CATGGTACAT 
TACTGCGAAA ACATAACAGA ATGCAGAAGA ATACAGCTTT TAGCTTACTT TGGTGAAAAA 
GGATTTAACC CTGATTTTTG TAAGAAACAT CCAGATGTTT CTTGTGACAA TTGCTGTAAA 
ACAAAGGATT ATAAAACAAA AGATGTGACT GATGATGTGA AAAATATTGT AAGATTTGTT 
CAAGAACACA GTTCATCACC AGGAACAAGA AATATAGGAC CTGCTGGAAG ATTTACTCTG 
AACATGCTAG TCGACATTTT CTTGGGAAGT AAGAGTGCAA AAGTTCAGTC TGGAATATTT 
GGAAAGGGGT CTGCATATTC ACGCCACAAT GCTGAAAGAC TTTTTAAAAA GCTGATTCTA 
GACAAAATCT TGGATGAAGA CTTATATATC AATGCCAATG ACCAACCAAT TGCCTATGTG 
ATGCTCGGAA CAAAAGCCCA CAGTGTGCTG AGCGGCCACT TGAAGGTGGA CTTCATGGAA 
ACGGAAAATT CGAGCAGTGT TAAAAAACAA AAAGCCGCAG TGGCCCACGT GTCCCAGAGA 
GACGAGGCAG TTATGAAGTG TCTGGGAGAA CTTGCAGACG TCTGCAAATT GCTGGGCAAA 
GTCTTTGGTG TCCATTACTT CAATATTTTC AATACAGCCA CCCTCAAAAA GCTTGCAGAA 
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAGATTGATG GTGTTACTGA AGACAAGCTG 
GAGAAATATG GTGCAGAAAT AATTCCAGTA TTACAGAAGT ACGCAGAGTG GACATTGCCA 
GCTGAAGGTG GGTCCCCAGG CAGCAGAGGC TCTAGGAGGA GCACCCCAGA GGAGACGGAG 
GAGGAGGAAG CTGTATCTTC TCACTACTTT GCAAATCAAA CTAGAAATGA AAGAAAGAGG 
AAGAAAGTGT CAGCCTCCCA AAAGCCCAAG AGAAGAAGAA CTAGTTACGG CGGCGGCTTC 
AGAGCAAAGG GGGGCTCTAC TACATGCAGA AAACCATCTT CTAAAAGTAA ATTCTCTGGT 
GTAACTGGAT CCCGCTCAGC CTCGTGTGCT TCTCAGGCAA CATCAGCAGC CAGTAGAAAA 
CTGGGGATTA TGGCTCCTCC AAAGCCTGTA AATAGACCGT TCCTCAGGCC TGCGTATGCC 
TTCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blm ( XM_017590166.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445655.1 Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X4, mRNA.

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CloneID	ORa56976
Clone ID Related Accession (Same CDS sequence)	XM_017590166.1
Accession Version	XM_017590166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Bloom syndrome protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGAAG AAGTTGACAG TGCAAAGAAG AGCCATGAAG ACAAGGCTGC ATTCCAGTCA 
CCTCAGAACG CTCTGTACTT TGAACACAAT GACAATGATT ATGATATAGA TTTTGTTCCA 
CCTTCTCCAG AAGAAATCAT TTCCACTGCT TCTTCCTCGT TGAAATGTTC CAGTATGCTA 
AAGGATCTCG ATGACTCTGA CAAAGAGAAG GACATTCTGA GTACCTCAAA AGATCTGTCA 
AAACCAGAGG AAATGACCAC ACCGAAGTCT GATCTGGGAA CCAATGAAGA CTGTGATGCC 
CAGCAGACTA GCTTGCAGCA GCAGCTTATC CGTGTGATGG AGCACATCTG TAAATTAGTT 
GATACTGTTC CTATTGATGA ACTGAAAGCT CTGACTTGTG GGGACAAACT GCTTCAGCAG 
CGAGACATAA GAAGGAAGCT CCTTGCTGAA GCAGGTTTTA ATAGAAACGA TGTCAATCTT 
CTGGGTTCTT TGTGGAGGCA CAGGCCAGAT TCGCTTGATA ACACTGTGCA GTGTGACTCC 
TGTCCTGTGG GGCATCCTGG TAAAGAGTTA AACTCTCCAT ACCTTCCCTC ACACTCCCTC 
TCCACTGAGG AATGCTTACC CACCACTGCT TCAGGAAAGA CAGGATTCTC AGCCACCCCG 
AAGAGTCTCT CTGAAAGGCC ACCATTGAAT TCCCACTTAC AGAAGTCCTT TGTAAGTAGC 
AACTGGGCTG AAACACCAAG GACAGAAAGC AGGACGGAAA GCACTTACTT CCCAGGGAGT 
GTTCTCACAA GCACCACTGT GAAGGATGAG AGTAAACATG TTGCTTCAGG ACGTGACATA 
GAGAGAGAAG TCCAGGCTTC CTACGATATT GATAATTTTA ACATTGATGA CTTTGATGAT 
GATGATGATG ATGACTGGGA AAACATAATG CACAATTTTT CAGTCAGCAA ATCTTCCACA 
GCTGCCTACC CACCTATCAA GGAAGGTGGC CCAGTTAAAT CTCTATCAGA AAGGATTTCT 
TCAGCCAAGG CAAAGTTTCT TCCAGTGGCA TCAACCGCTC AAAATAAAAA CCTCTCAGAG 
TCAATTCAGA ATTGCTCTGA TAAGTTTGCA CAAAATCTAT CATCAAAAAA TCCAAAACAT 
GAACATTTTC AAAGTCTTAA TTTTCCTCAT ACAAAAGAAA TGATGAAGAT TTTCCGTAAG 
AAATTTGGCT TGCATAATTT TAGAACTAAT CAGCTAGAGG CGATCAATGC TGCGCTGCTT 
GGTGAAGACT GCTTTATCCT GATGCCCACT GGAGGTGGTA AAAGTTTGTG CTACCAGCTC 
CCTGCCTGTG TTTCTCCTGG AGTCACTATT GTCATTTCTC CCTTGAGATC ACTAATAGTG 
GACCAAGTCC AAAAGCTGAC TTCCTTTGAT ATTCCAGCTA CGTACCTGAC AGGGGATAAG 
ACTGACTCCG AAGCTGCAAA CATTTACCTC CAGTTGTCAA AGAAAGACCC GATCATCAAG 
CTTCTGTATG TCACCCCAGA GAAGGTCTGT GCAAGTAACA GGCTGATTTC CACTCTGGAG 
AATCTGTATG AGCGAAAGCT CTTGGCACGA TTTGTCATTG ATGAAGCACA CTGTGTGAGT 
CAGTGGGGCC ATGATTTCCG TCAAGATTAC AAAAGGATGA ATATGCTTCG CCAGAGGTTC 
CCTTCTGTCC CAGTGATGGC TCTCACGGCC ACAGCGAACC CCAGGGTACA GAAGGACATC 
CTCACGCAGC TGAAGATTCT CAGGCCTCAG GTGTTTAGCA TGAGCTTTAA CAGACACAAT 
CTGAAATACT ATGTATTACC AAAGAAGCCC AAGAAAGTGG CACTTGATTG CCTAGAGTGG 
ATCAGAAAGC ATCACCCTTA TGACTCGGGG ATAATTTATT GCCTCTCCAG GAAGGAATGT 
GACACAATGG CCGACACCTT ACAGAGAGAA GGTCTTGCTG CTCTGGCTTA CCATGCAGGT 
CTTAGTGATT CTGCCAGAGA TGAGGTGCAG CACAAGTGGA TCAACCAGGA CAACTGCCAG 
GTTATCTGTG CAACCATTGC ATTTGGAATG GGAATCGACA AACCTGACGT GCGATTTGTG 
GTTCATGCAT CTCTTCCTAA ATCTGTGGAG GGTTATTACC AAGAATCCGG CCGAGCTGGG 
AGAGACGGGG AAATATCTCA CTGCGTGCTT TTCTATACCT ATCATGACGT GACCAGACTG 
AAGAGACTTA TAATGATGGA AAAAGATGGA AACTATCATA CAAGGGAAAC TCACGTCAAT 
AATTTATATA GCATGGTACA TTACTGCGAA AACATAACAG AATGCAGAAG AATACAGCTT 
TTAGCTTACT TTGGTGAAAA AGGATTTAAC CCTGATTTTT GTAAGAAACA TCCAGATGTT 
TCTTGTGACA ATTGCTGTAA AACAAAGGAT TATAAAACAA AAGATGTGAC TGATGATGTG 
AAAAATATTG TAAGATTTGT TCAAGAACAC AGTTCATCAC CAGGAACAAG AAATATAGGA 
CCTGCTGGAA GATTTACTCT GAACATGCTA GTCGACATTT TCTTGGGAAG TAAGAGTGCA 
AAAGTTCAGT CTGGAATATT TGGAAAGGGG TCTGCATATT CACGCCACAA TGCTGAAAGA 
CTTTTTAAAA AGCTGATTCT AGACAAAATC TTGGATGAAG ACTTATATAT CAATGCCAAT 
GACCAACCAA TTGCCTATGT GATGCTCGGA ACAAAAGCCC ACAGTGTGCT GAGCGGCCAC 
TTGAAGGTGG ACTTCATGGA AACGGAAAAT TCGAGCAGTG TTAAAAAACA AAAAGCCGCA 
GTGGCCCACG TGTCCCAGAG AGACGAGGCA GTTATGAAGT GTCTGGGAGA ACTTGCAGAC 
GTCTGCAAAT TGCTGGGCAA AGTCTTTGGT GTCCATTACT TCAATATTTT CAATACAGCC 
ACCCTCAAAA AGCTTGCAGA ATCTTTATCT TCTGATCCTG AGGTTTTGCT TCAGATTGAT 
GGTGTTACTG AAGACAAGCT GGAGAAATAT GGTGCAGAAA TAATTCCAGT ATTACAGAAG 
TACGCAGAGT GGACATTGCC AGCTGAAGGT GGGTCCCCAG GCAGCAGAGG CTCTAGGAGG 
AGCACCCCAG AGGAGACGGA GGAGGAGGAA GCTGTATCTT CTCACTACTT TGCAAATCAA 
ACTAGAAATG AAAGAAAGAG GAAGAAAGTG TCAGCCTCCC AAAAGCCCAA GAGAAGAAGA 
ACTAGTTACG GCGGCGGCTT CAGAGCAAAG GGGGGCTCTA CTACATGCAG AAAACCATCT 
TCTAAAAGTA AATTCTCTGG TGTAACTGGA TCCCGCTCAG CCTCGTGTGC TTCTCAGGCA 
ACATCAGCAG CCAGTAGAAA ACTGGGGATT ATGGCTCCTC CAAAGCCTGT AAATAGACCG 
TTCCTCAGGC CTGCGTATGC CTTCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445655.1
CDS	124..3573
Translation	MHEEVDSAKKSHEDKAAFQSPQNALYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKDILSTSKDLSKPEEMTTPKSDLGTNEDCDAQQTSLQQQLIRVMEHICKLVDTVPIDELKALTCGDKLLQQRDIRRKLLAEAGFNRNDVNLLGSLWRHRPDSLDNTVQCDSCPVGHPGKELNSPYLPSHSLSTEECLPTTASGKTGFSATPKSLSERPPLNSHLQKSFVSSNWAETPRTESRTESTYFPGSVLTSTTVKDESKHVASGRDIEREVQASYDIDNFNIDDFDDDDDDDWENIMHNFSVSKSSTAAYPPIKEGGPVKSLSERISSAKAKFLPVASTAQNKNLSESIQNCSDKFAQNLSSKNPKHEHFQSLNFPHTKEMMKIFRKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQRFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVALDCLEWIRKHHPYDSGIIYCLSRKECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVVHASLPKSVEGYYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTRETHVNNLYSMVHYCENITECRRIQLLAYFGEKGFNPDFCKKHPDVSCDNCCKTKDYKTKDVTDDVKNIVRFVQEHSSSPGTRNIGPAGRFTLNMLVDIFLGSKSAKVQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHLKVDFMETENSSSVKKQKAAVAHVSQRDEAVMKCLGELADVCKLLGKVFGVHYFNIFNTATLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEIIPVLQKYAEWTLPAEGGSPGSRGSRRSTPEETEEEEAVSSHYFANQTRNERKRKKVSASQKPKRRRTSYGGGFRAKGGSTTCRKPSSKSKFSGVTGSRSASCASQATSAASRKLGIMAPPKPVNRPFLRPAYAFS

Target ORF information:

RefSeq Version	XM_017590166.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590166.1

ORF Insert Sequence:

ATGCATGAAG AAGTTGACAG TGCAAAGAAG AGCCATGAAG ACAAGGCTGC ATTCCAGTCA 
CCTCAGAACG CTCTGTACTT TGAACACAAT GACAATGATT ATGATATAGA TTTTGTTCCA 
CCTTCTCCAG AAGAAATCAT TTCCACTGCT TCTTCCTCGT TGAAATGTTC CAGTATGCTA 
AAGGATCTCG ATGACTCTGA CAAAGAGAAG GACATTCTGA GTACCTCAAA AGATCTGTCA 
AAACCAGAGG AAATGACCAC ACCGAAGTCT GATCTGGGAA CCAATGAAGA CTGTGATGCC 
CAGCAGACTA GCTTGCAGCA GCAGCTTATC CGTGTGATGG AGCACATCTG TAAATTAGTT 
GATACTGTTC CTATTGATGA ACTGAAAGCT CTGACTTGTG GGGACAAACT GCTTCAGCAG 
CGAGACATAA GAAGGAAGCT CCTTGCTGAA GCAGGTTTTA ATAGAAACGA TGTCAATCTT 
CTGGGTTCTT TGTGGAGGCA CAGGCCAGAT TCGCTTGATA ACACTGTGCA GTGTGACTCC 
TGTCCTGTGG GGCATCCTGG TAAAGAGTTA AACTCTCCAT ACCTTCCCTC ACACTCCCTC 
TCCACTGAGG AATGCTTACC CACCACTGCT TCAGGAAAGA CAGGATTCTC AGCCACCCCG 
AAGAGTCTCT CTGAAAGGCC ACCATTGAAT TCCCACTTAC AGAAGTCCTT TGTAAGTAGC 
AACTGGGCTG AAACACCAAG GACAGAAAGC AGGACGGAAA GCACTTACTT CCCAGGGAGT 
GTTCTCACAA GCACCACTGT GAAGGATGAG AGTAAACATG TTGCTTCAGG ACGTGACATA 
GAGAGAGAAG TCCAGGCTTC CTACGATATT GATAATTTTA ACATTGATGA CTTTGATGAT 
GATGATGATG ATGACTGGGA AAACATAATG CACAATTTTT CAGTCAGCAA ATCTTCCACA 
GCTGCCTACC CACCTATCAA GGAAGGTGGC CCAGTTAAAT CTCTATCAGA AAGGATTTCT 
TCAGCCAAGG CAAAGTTTCT TCCAGTGGCA TCAACCGCTC AAAATAAAAA CCTCTCAGAG 
TCAATTCAGA ATTGCTCTGA TAAGTTTGCA CAAAATCTAT CATCAAAAAA TCCAAAACAT 
GAACATTTTC AAAGTCTTAA TTTTCCTCAT ACAAAAGAAA TGATGAAGAT TTTCCGTAAG 
AAATTTGGCT TGCATAATTT TAGAACTAAT CAGCTAGAGG CGATCAATGC TGCGCTGCTT 
GGTGAAGACT GCTTTATCCT GATGCCCACT GGAGGTGGTA AAAGTTTGTG CTACCAGCTC 
CCTGCCTGTG TTTCTCCTGG AGTCACTATT GTCATTTCTC CCTTGAGATC ACTAATAGTG 
GACCAAGTCC AAAAGCTGAC TTCCTTTGAT ATTCCAGCTA CGTACCTGAC AGGGGATAAG 
ACTGACTCCG AAGCTGCAAA CATTTACCTC CAGTTGTCAA AGAAAGACCC GATCATCAAG 
CTTCTGTATG TCACCCCAGA GAAGGTCTGT GCAAGTAACA GGCTGATTTC CACTCTGGAG 
AATCTGTATG AGCGAAAGCT CTTGGCACGA TTTGTCATTG ATGAAGCACA CTGTGTGAGT 
CAGTGGGGCC ATGATTTCCG TCAAGATTAC AAAAGGATGA ATATGCTTCG CCAGAGGTTC 
CCTTCTGTCC CAGTGATGGC TCTCACGGCC ACAGCGAACC CCAGGGTACA GAAGGACATC 
CTCACGCAGC TGAAGATTCT CAGGCCTCAG GTGTTTAGCA TGAGCTTTAA CAGACACAAT 
CTGAAATACT ATGTATTACC AAAGAAGCCC AAGAAAGTGG CACTTGATTG CCTAGAGTGG 
ATCAGAAAGC ATCACCCTTA TGACTCGGGG ATAATTTATT GCCTCTCCAG GAAGGAATGT 
GACACAATGG CCGACACCTT ACAGAGAGAA GGTCTTGCTG CTCTGGCTTA CCATGCAGGT 
CTTAGTGATT CTGCCAGAGA TGAGGTGCAG CACAAGTGGA TCAACCAGGA CAACTGCCAG 
GTTATCTGTG CAACCATTGC ATTTGGAATG GGAATCGACA AACCTGACGT GCGATTTGTG 
GTTCATGCAT CTCTTCCTAA ATCTGTGGAG GGTTATTACC AAGAATCCGG CCGAGCTGGG 
AGAGACGGGG AAATATCTCA CTGCGTGCTT TTCTATACCT ATCATGACGT GACCAGACTG 
AAGAGACTTA TAATGATGGA AAAAGATGGA AACTATCATA CAAGGGAAAC TCACGTCAAT 
AATTTATATA GCATGGTACA TTACTGCGAA AACATAACAG AATGCAGAAG AATACAGCTT 
TTAGCTTACT TTGGTGAAAA AGGATTTAAC CCTGATTTTT GTAAGAAACA TCCAGATGTT 
TCTTGTGACA ATTGCTGTAA AACAAAGGAT TATAAAACAA AAGATGTGAC TGATGATGTG 
AAAAATATTG TAAGATTTGT TCAAGAACAC AGTTCATCAC CAGGAACAAG AAATATAGGA 
CCTGCTGGAA GATTTACTCT GAACATGCTA GTCGACATTT TCTTGGGAAG TAAGAGTGCA 
AAAGTTCAGT CTGGAATATT TGGAAAGGGG TCTGCATATT CACGCCACAA TGCTGAAAGA 
CTTTTTAAAA AGCTGATTCT AGACAAAATC TTGGATGAAG ACTTATATAT CAATGCCAAT 
GACCAACCAA TTGCCTATGT GATGCTCGGA ACAAAAGCCC ACAGTGTGCT GAGCGGCCAC 
TTGAAGGTGG ACTTCATGGA AACGGAAAAT TCGAGCAGTG TTAAAAAACA AAAAGCCGCA 
GTGGCCCACG TGTCCCAGAG AGACGAGGCA GTTATGAAGT GTCTGGGAGA ACTTGCAGAC 
GTCTGCAAAT TGCTGGGCAA AGTCTTTGGT GTCCATTACT TCAATATTTT CAATACAGCC 
ACCCTCAAAA AGCTTGCAGA ATCTTTATCT TCTGATCCTG AGGTTTTGCT TCAGATTGAT 
GGTGTTACTG AAGACAAGCT GGAGAAATAT GGTGCAGAAA TAATTCCAGT ATTACAGAAG 
TACGCAGAGT GGACATTGCC AGCTGAAGGT GGGTCCCCAG GCAGCAGAGG CTCTAGGAGG 
AGCACCCCAG AGGAGACGGA GGAGGAGGAA GCTGTATCTT CTCACTACTT TGCAAATCAA 
ACTAGAAATG AAAGAAAGAG GAAGAAAGTG TCAGCCTCCC AAAAGCCCAA GAGAAGAAGA 
ACTAGTTACG GCGGCGGCTT CAGAGCAAAG GGGGGCTCTA CTACATGCAG AAAACCATCT 
TCTAAAAGTA AATTCTCTGG TGTAACTGGA TCCCGCTCAG CCTCGTGTGC TTCTCAGGCA 
ACATCAGCAG CCAGTAGAAA ACTGGGGATT ATGGCTCCTC CAAAGCCTGT AAATAGACCG 
TTCCTCAGGC CTGCGTATGC CTTCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blm ( XM_006223359.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006223421.1 Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa14498
Clone ID Related Accession (Same CDS sequence)	XM_006223359.3
Accession Version	XM_006223359.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Bloom syndrome protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006223359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAT CTCCCGTTGG TTGGATCATG GCTGCTGTTC CTCTGAACAA TCTACAAGAA 
CAACTACAGC GTCACTCGGC CAGAAAACTT AAAAATCAGT TCAGCGTTTC AAAACCAAAA 
CCTTCAGGTT TTACTTTTAA AAAGAAAACA CCAGAGGGTG ATGTGTCTGT GGTAAGAACA 
CCTGTGTTAA GCGATAAAGA TGTGAATATG TCTGAGGCCT TGTCATATAC TGAGCCTCCA 
CTCCACAAAC CAAAGCAGCA GTCAAACATC GATGGCTTCT TTAAAAATTC CCCTGGAGGG 
CAGCAAAGCA AGGAGACCTG CTCCGAACTG TCCCTGCCGG AGGTGGTACA GATTCCTCAG 
GACGCTTCTT GTACTACCCC AAAAACCCCA GCTGTGAAGA AACCCCACGT GGCCATTTTC 
AAAAAACTGG AATTTAGTTC TTCGGCAGAC TCTCTCAGTG ATTGGGCTGA TATAGATGAC 
TTTGATGTGC CAGCATCAGA CGCATTTGTT TCCCTGACCA GAAACCCTAC TACAAGAGTG 
AGCACTGCTA AGAAACTGAG TAGCAGTAAG AGAAACTTCT TTAAACCACC ACCTAGAGCA 
AGTGCAGTAA AGGCCGACTT GACTCCTTCC TCCCCCGAAT GCCTGCAAGT GGATTTAACG 
AAGGAACAGG AGGAAGAGGC GGACTGCCTG AGCAGGGATG TGATCTGCAT TGACACTGAC 
CCTGCTTCTG AGGAGCTCTT GGAGAAGGAT GTGCAGGAAG GCCTGTCATT GGACGCTCGC 
TTGGGGACTG AAAGAGGTAT TGACAGTGCA AAGAAGAGCC ATGAAGACGA GGCTGCATTC 
CAGTCACCTC AGAACGCTCT GTACTTTGAA CACAATGACA ATGATTATGA TATAGATTTT 
GTTCCACCTT CTCCAGAAGA AATCATTTCC ACTGCTTCTT CCTCGTTGAA ATGTTCCAGT 
ATGCTAAAGG ATCTCGATGA CTCTGACAAA GAGAAGGACA TTCTGAGTAC CTCAAAAGAT 
CTGTCAAAAC CAGAGGAAAT GACCACACCG AAGTCTGATC TGGGAACCAA TGAAGACTGT 
GATGCCCAGC AGACTAGCTT GCAGCAGCAG CTTATCCGTG TGATGGAGCA CATCTGTAAA 
TTAGTTGATA CTGTTCCTAT TGATGAACTG AAAGCTCTGA CTTGTGGGGA CAAACTGCTT 
CAGCAGCGAG ACATAAGAAG GAAGCTCCTT GCTGAAGCAG GTTTTAATAG AAACGATGTC 
AATCTTCTGG GTTCTTTGTG GAGGCACAGG CCAGATTCGC TTGATAACAC TGTGCAGTGT 
GACTCCTGTC CTGTGGGGCA TCCTGGTAAA GAGTTAAACT CTCCATACCT TCCCTCACAC 
TCCCTCTCCA CTGAGGAATG CTTACCCACC ACTGCTTCAG GAAAGACAGG ATTCTCAGCC 
ACCCCGAAGA GTCTCTCTGA AAGGCCACCA TTGAATTCCC ACTTACAGAA GTCCTTTGTA 
AGTAGCAACT GGGCTGAAAC ACCAAGGACA GAAAGCAGGA CGGAAAGCAC TTACTTCCCA 
GGGAGTGTTC TCACAAGCAC CACTGTGAAG GATGAGAGTA AACATGTTGC TTCAGGACGT 
GACATAGAGA GAGAAGTCCA GGCTTCCTAC GATATTGATA ATTTTAACAT TGATGACTTT 
GATGATGATG ATGATGATGA CTGGGAAAAC ATAATGCACA ATTTTTCAGT CAGCAAATCT 
TCCACAGCTG CCTACCCACC TATCAAGGAA GGTGGCCCAG TTAAATCTCT ATCAGAAAGG 
ATTTCTTCAG CCAAGGCAAA GTTTCTTCCA GTGGCATCAA CCGCTCAAAA TAAAAACCTC 
TCAGAGTCAA TTCAGAATTG CTCTGATAAG TTTGCACAAA ATCTATCATC AAAAAATCCA 
AAACATGAAC ATTTTCAAAG TCTTAATTTT CCTCATACAA AAGAAATGAT GAAGATTTTC 
CGTAAGAAAT TTGGCTTGCA TAATTTTAGA ACTAATCAGC TAGAGGCGAT CAATGCTGCG 
CTGCTTGGTG AAGACTGCTT TATCCTGATG CCCACTGGAG GTGGTAAAAG TTTGTGCTAC 
CAGCTCCCTG CCTGTGTTTC TCCTGGAGTC ACTATTGTCA TTTCTCCCTT GAGATCACTA 
ATAGTGGACC AAGTCCAAAA GCTGACTTCC TTTGATATTC CAGCTACGTA CCTGACAGGG 
GATAAGACTG ACTCCGAAGC TGCAAACATT TACCTCCAGT TGTCAAAGAA AGACCCGATC 
ATCAAGCTTC TGTATGTCAC CCCAGAGAAG GTCTGTGCAA GTAACAGGCT GATTTCCACT 
CTGGAGAATC TGTATGAGCG GAAGCTCTTG GCACGATTTG TCATTGATGA AGCACACTGT 
GTGAGTCAGT GGGGCCATGA TTTCCGTCAA GATTACAAAA GGATGAATAT GCTTCGCCAG 
AGGTTCCCTT CTGTCCCAGT GATGGCTCTC ACGGCCACAG CGAACCCCAG GGTACAGAAG 
GACATCCTCA CGCAGCTGAA GATTCTCAGG CCTCAGGTGT TTAGCATGAG CTTTAACAGA 
CACAATCTGA AATACTATGT ATTACCAAAG AAGCCCAAGA AAGTGGCACT TGATTGCCTA 
GAGTGGATCA GAAAGCATCA CCCTTATGAC TCGGGGATAA TTTATTGCCT CTCCAGGAAG 
GAATGTGACA CAATGGCCGA CACCTTACAG AGAGAAGGTC TTGCTGCTCT GGCTTACCAT 
GCAGGTCTTA GTGATTCTGC CAGAGATGAG GTGCAGCACA AGTGGATCAA CCAGGACAAC 
TGCCAGGTTA TCTGTGCAAC CATTGCATTT GGAATGGGAA TCGACAAACC TGACGTGCGA 
TTTGTGGTTC ATGCATCTCT TCCTAAATCT GTGGAGGGTT ATTACCAAGA ATCCGGCCGA 
GCTGGGAGAG ACGGGGAAAT ATCTCACTGC GTGCTTTTCT ATACCTATCA TGACGTGACC 
AGACTGAAGA GACTTATAAT GATGGAAAAA GATGGAAACT ATCATACAAG GGAAACTCAC 
GTCAATAATT TATATAGCAT GGTACATTAC TGCGAAAACA TAACAGAATG CAGAAGAATA 
CAGCTTTTAG CTTACTTTGG TGAAAAAGGA TTTAACCCTG ATTTTTGTAA GAAACATCCA 
GATGTTTCTT GTGACAATTG CTGTAAAACA AAGGATTATA AAACAAAAGA TGTGACTGAT 
GATGTGAAAA ATATTGTAAG ATTTGTTCAA GAACACAGTT CATCACCAGG AACAAGAAAT 
ATAGGACCTG CTGGAAGATT TACTCTGAAC ATGCTAGTCG ACATTTTCTT GGGAAGTAAG 
AGTGCAAAAG TTCAGTCTGG AATATTTGGA AAGGGGTCTG CATATTCACG CCACAATGCT 
GAAAGACTTT TTAAAAAGCT GATTCTAGAC AAAATCTTGG ATGAAGACTT ATATATCAAT 
GCCAATGACC AACCAATTGC CTATGTGATG CTCGGAACAA AAGCCCACAG TGTGCTGAGC 
GGCCACTTGA AGGTGGACTT CATGGAAACG GAAAATTCGA GCAGTGTTAA AAAACAAAAA 
GCCGCAGTGG CCCACGTGTC CCAGAGAGAC GAGGCAGTTA TGAAGTGTCT GGGAGAACTT 
GCAGACGTCT GCAAATTGCT GGGCAAAGTC TTTGGTGTCC ATTACTTCAA TATTTTCAAT 
ACAGCCACCC TCAAAAAGCT TGCAGAATCT TTATCTTCTG ATCCTGAGGT TTTGCTTCAG 
ATTGATGGTG TTACTGAAGA CAAGCTGGAG AAATATGGTG CAGAAATAAT TCCAGTATTA 
CAGAAGTACG CAGAGTGGAC ATTGCCAGCT GAAGGTGGGT CCCCAGGCAG CAGAGGCTCT 
AGGAGGAGCA CCCCAGAGGA GACGGAGGAG GAGGAAGCTG TATCTTCTCA CTACTTTGCA 
AATCAAACTA GAAATGAAAG AAAGAGGAAG AAAGTGTCAG CCTCCCAAAA GCCCAAGAGA 
AGAAGAACTA GTTACGGCGG CGGCTTCAGA GCAAAGGGGG GCTCTACTAC ATGCAGAAAA 
CCATCTTCTA AAAGTAAATT CTCTGGTGTA ACTGGATCCC GCTCAGCCTC GTGTGCTTCT 
CAGGCAACAT CAGCAGCCAG TAGAAAACTG GGGATTATGG CTCCTCCAAA GCCTGTAAAT 
AGACCGTTCC TCAGGCCTGC GTATGCCTTC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006223421.1
CDS	354..4589
Translation	MFGSPVGWIMAAVPLNNLQEQLQRHSARKLKNQFSVSKPKPSGFTFKKKTPEGDVSVVRTPVLSDKDVNMSEALSYTEPPLHKPKQQSNIDGFFKNSPGGQQSKETCSELSLPEVVQIPQDASCTTPKTPAVKKPHVAIFKKLEFSSSADSLSDWADIDDFDVPASDAFVSLTRNPTTRVSTAKKLSSSKRNFFKPPPRASAVKADLTPSSPECLQVDLTKEQEEEADCLSRDVICIDTDPASEELLEKDVQEGLSLDARLGTERGIDSAKKSHEDEAAFQSPQNALYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKDILSTSKDLSKPEEMTTPKSDLGTNEDCDAQQTSLQQQLIRVMEHICKLVDTVPIDELKALTCGDKLLQQRDIRRKLLAEAGFNRNDVNLLGSLWRHRPDSLDNTVQCDSCPVGHPGKELNSPYLPSHSLSTEECLPTTASGKTGFSATPKSLSERPPLNSHLQKSFVSSNWAETPRTESRTESTYFPGSVLTSTTVKDESKHVASGRDIEREVQASYDIDNFNIDDFDDDDDDDWENIMHNFSVSKSSTAAYPPIKEGGPVKSLSERISSAKAKFLPVASTAQNKNLSESIQNCSDKFAQNLSSKNPKHEHFQSLNFPHTKEMMKIFRKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQRFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVALDCLEWIRKHHPYDSGIIYCLSRKECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVVHASLPKSVEGYYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTRETHVNNLYSMVHYCENITECRRIQLLAYFGEKGFNPDFCKKHPDVSCDNCCKTKDYKTKDVTDDVKNIVRFVQEHSSSPGTRNIGPAGRFTLNMLVDIFLGSKSAKVQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHLKVDFMETENSSSVKKQKAAVAHVSQRDEAVMKCLGELADVCKLLGKVFGVHYFNIFNTATLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEIIPVLQKYAEWTLPAEGGSPGSRGSRRSTPEETEEEEAVSSHYFANQTRNERKRKKVSASQKPKRRRTSYGGGFRAKGGSTTCRKPSSKSKFSGVTGSRSASCASQATSAASRKLGIMAPPKPVNRPFLRPAYAFS

Target ORF information:

RefSeq Version	XM_006223359.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006223359.3

ORF Insert Sequence:

ATGTTTGGAT CTCCCGTTGG TTGGATCATG GCTGCTGTTC CTCTGAACAA TCTACAAGAA 
CAACTACAGC GTCACTCGGC CAGAAAACTT AAAAATCAGT TCAGCGTTTC AAAACCAAAA 
CCTTCAGGTT TTACTTTTAA AAAGAAAACA CCAGAGGGTG ATGTGTCTGT GGTAAGAACA 
CCTGTGTTAA GCGATAAAGA TGTGAATATG TCTGAGGCCT TGTCATATAC TGAGCCTCCA 
CTCCACAAAC CAAAGCAGCA GTCAAACATC GATGGCTTCT TTAAAAATTC CCCTGGAGGG 
CAGCAAAGCA AGGAGACCTG CTCCGAACTG TCCCTGCCGG AGGTGGTACA GATTCCTCAG 
GACGCTTCTT GTACTACCCC AAAAACCCCA GCTGTGAAGA AACCCCACGT GGCCATTTTC 
AAAAAACTGG AATTTAGTTC TTCGGCAGAC TCTCTCAGTG ATTGGGCTGA TATAGATGAC 
TTTGATGTGC CAGCATCAGA CGCATTTGTT TCCCTGACCA GAAACCCTAC TACAAGAGTG 
AGCACTGCTA AGAAACTGAG TAGCAGTAAG AGAAACTTCT TTAAACCACC ACCTAGAGCA 
AGTGCAGTAA AGGCCGACTT GACTCCTTCC TCCCCCGAAT GCCTGCAAGT GGATTTAACG 
AAGGAACAGG AGGAAGAGGC GGACTGCCTG AGCAGGGATG TGATCTGCAT TGACACTGAC 
CCTGCTTCTG AGGAGCTCTT GGAGAAGGAT GTGCAGGAAG GCCTGTCATT GGACGCTCGC 
TTGGGGACTG AAAGAGGTAT TGACAGTGCA AAGAAGAGCC ATGAAGACGA GGCTGCATTC 
CAGTCACCTC AGAACGCTCT GTACTTTGAA CACAATGACA ATGATTATGA TATAGATTTT 
GTTCCACCTT CTCCAGAAGA AATCATTTCC ACTGCTTCTT CCTCGTTGAA ATGTTCCAGT 
ATGCTAAAGG ATCTCGATGA CTCTGACAAA GAGAAGGACA TTCTGAGTAC CTCAAAAGAT 
CTGTCAAAAC CAGAGGAAAT GACCACACCG AAGTCTGATC TGGGAACCAA TGAAGACTGT 
GATGCCCAGC AGACTAGCTT GCAGCAGCAG CTTATCCGTG TGATGGAGCA CATCTGTAAA 
TTAGTTGATA CTGTTCCTAT TGATGAACTG AAAGCTCTGA CTTGTGGGGA CAAACTGCTT 
CAGCAGCGAG ACATAAGAAG GAAGCTCCTT GCTGAAGCAG GTTTTAATAG AAACGATGTC 
AATCTTCTGG GTTCTTTGTG GAGGCACAGG CCAGATTCGC TTGATAACAC TGTGCAGTGT 
GACTCCTGTC CTGTGGGGCA TCCTGGTAAA GAGTTAAACT CTCCATACCT TCCCTCACAC 
TCCCTCTCCA CTGAGGAATG CTTACCCACC ACTGCTTCAG GAAAGACAGG ATTCTCAGCC 
ACCCCGAAGA GTCTCTCTGA AAGGCCACCA TTGAATTCCC ACTTACAGAA GTCCTTTGTA 
AGTAGCAACT GGGCTGAAAC ACCAAGGACA GAAAGCAGGA CGGAAAGCAC TTACTTCCCA 
GGGAGTGTTC TCACAAGCAC CACTGTGAAG GATGAGAGTA AACATGTTGC TTCAGGACGT 
GACATAGAGA GAGAAGTCCA GGCTTCCTAC GATATTGATA ATTTTAACAT TGATGACTTT 
GATGATGATG ATGATGATGA CTGGGAAAAC ATAATGCACA ATTTTTCAGT CAGCAAATCT 
TCCACAGCTG CCTACCCACC TATCAAGGAA GGTGGCCCAG TTAAATCTCT ATCAGAAAGG 
ATTTCTTCAG CCAAGGCAAA GTTTCTTCCA GTGGCATCAA CCGCTCAAAA TAAAAACCTC 
TCAGAGTCAA TTCAGAATTG CTCTGATAAG TTTGCACAAA ATCTATCATC AAAAAATCCA 
AAACATGAAC ATTTTCAAAG TCTTAATTTT CCTCATACAA AAGAAATGAT GAAGATTTTC 
CGTAAGAAAT TTGGCTTGCA TAATTTTAGA ACTAATCAGC TAGAGGCGAT CAATGCTGCG 
CTGCTTGGTG AAGACTGCTT TATCCTGATG CCCACTGGAG GTGGTAAAAG TTTGTGCTAC 
CAGCTCCCTG CCTGTGTTTC TCCTGGAGTC ACTATTGTCA TTTCTCCCTT GAGATCACTA 
ATAGTGGACC AAGTCCAAAA GCTGACTTCC TTTGATATTC CAGCTACGTA CCTGACAGGG 
GATAAGACTG ACTCCGAAGC TGCAAACATT TACCTCCAGT TGTCAAAGAA AGACCCGATC 
ATCAAGCTTC TGTATGTCAC CCCAGAGAAG GTCTGTGCAA GTAACAGGCT GATTTCCACT 
CTGGAGAATC TGTATGAGCG GAAGCTCTTG GCACGATTTG TCATTGATGA AGCACACTGT 
GTGAGTCAGT GGGGCCATGA TTTCCGTCAA GATTACAAAA GGATGAATAT GCTTCGCCAG 
AGGTTCCCTT CTGTCCCAGT GATGGCTCTC ACGGCCACAG CGAACCCCAG GGTACAGAAG 
GACATCCTCA CGCAGCTGAA GATTCTCAGG CCTCAGGTGT TTAGCATGAG CTTTAACAGA 
CACAATCTGA AATACTATGT ATTACCAAAG AAGCCCAAGA AAGTGGCACT TGATTGCCTA 
GAGTGGATCA GAAAGCATCA CCCTTATGAC TCGGGGATAA TTTATTGCCT CTCCAGGAAG 
GAATGTGACA CAATGGCCGA CACCTTACAG AGAGAAGGTC TTGCTGCTCT GGCTTACCAT 
GCAGGTCTTA GTGATTCTGC CAGAGATGAG GTGCAGCACA AGTGGATCAA CCAGGACAAC 
TGCCAGGTTA TCTGTGCAAC CATTGCATTT GGAATGGGAA TCGACAAACC TGACGTGCGA 
TTTGTGGTTC ATGCATCTCT TCCTAAATCT GTGGAGGGTT ATTACCAAGA ATCCGGCCGA 
GCTGGGAGAG ACGGGGAAAT ATCTCACTGC GTGCTTTTCT ATACCTATCA TGACGTGACC 
AGACTGAAGA GACTTATAAT GATGGAAAAA GATGGAAACT ATCATACAAG GGAAACTCAC 
GTCAATAATT TATATAGCAT GGTACATTAC TGCGAAAACA TAACAGAATG CAGAAGAATA 
CAGCTTTTAG CTTACTTTGG TGAAAAAGGA TTTAACCCTG ATTTTTGTAA GAAACATCCA 
GATGTTTCTT GTGACAATTG CTGTAAAACA AAGGATTATA AAACAAAAGA TGTGACTGAT 
GATGTGAAAA ATATTGTAAG ATTTGTTCAA GAACACAGTT CATCACCAGG AACAAGAAAT 
ATAGGACCTG CTGGAAGATT TACTCTGAAC ATGCTAGTCG ACATTTTCTT GGGAAGTAAG 
AGTGCAAAAG TTCAGTCTGG AATATTTGGA AAGGGGTCTG CATATTCACG CCACAATGCT 
GAAAGACTTT TTAAAAAGCT GATTCTAGAC AAAATCTTGG ATGAAGACTT ATATATCAAT 
GCCAATGACC AACCAATTGC CTATGTGATG CTCGGAACAA AAGCCCACAG TGTGCTGAGC 
GGCCACTTGA AGGTGGACTT CATGGAAACG GAAAATTCGA GCAGTGTTAA AAAACAAAAA 
GCCGCAGTGG CCCACGTGTC CCAGAGAGAC GAGGCAGTTA TGAAGTGTCT GGGAGAACTT 
GCAGACGTCT GCAAATTGCT GGGCAAAGTC TTTGGTGTCC ATTACTTCAA TATTTTCAAT 
ACAGCCACCC TCAAAAAGCT TGCAGAATCT TTATCTTCTG ATCCTGAGGT TTTGCTTCAG 
ATTGATGGTG TTACTGAAGA CAAGCTGGAG AAATATGGTG CAGAAATAAT TCCAGTATTA 
CAGAAGTACG CAGAGTGGAC ATTGCCAGCT GAAGGTGGGT CCCCAGGCAG CAGAGGCTCT 
AGGAGGAGCA CCCCAGAGGA GACGGAGGAG GAGGAAGCTG TATCTTCTCA CTACTTTGCA 
AATCAAACTA GAAATGAAAG AAAGAGGAAG AAAGTGTCAG CCTCCCAAAA GCCCAAGAGA 
AGAAGAACTA GTTACGGCGG CGGCTTCAGA GCAAAGGGGG GCTCTACTAC ATGCAGAAAA 
CCATCTTCTA AAAGTAAATT CTCTGGTGTA ACTGGATCCC GCTCAGCCTC GTGTGCTTCT 
CAGGCAACAT CAGCAGCCAG TAGAAAACTG GGGATTATGG CTCCTCCAAA GCCTGTAAAT 
AGACCGTTCC TCAGGCCTGC GTATGCCTTC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blm ( XM_003753301.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_003753349.2 Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X2, mRNA.

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CloneID	ORa14499
Clone ID Related Accession (Same CDS sequence)	XM_003753301.4
Accession Version	XM_003753301.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Bloom syndrome protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_003753301.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAT CTCCCGTTGG TTGGATCATG GCTGCTGTTC CTCTGAACAA TCTACAAGAA 
CAACTACAGC GTCACTCGGC CAGAAAACTT AAAAATCAGT TCAGCGTTTC AAAACCAAAA 
CCTTCAGGTT TTACTTTTAA AAAGAAAACA CCAGAGGGTG ATGTGTCTGT GGTAAGAACA 
CCTGTGTTAA GCGATAAAGA TGTGAATATG TCTGAGGCCT TGTCATATAC TGAGCCTCCA 
CTCCACAAAC CAAAGCAGCA GTCAAACATC GATGGCTTCT TTAAAAATTC CCCTGGAGGG 
CAGCAAAGCA AGGAGACCTG CTCCGAACTG TCCCTGCCGG AGGTGGTACA GATTCCTCAG 
GACGCTTCTT GTACTACCCC AAAAACCCCA GCTGTGAAGA AACCCCACGT GGCCATTTTC 
AAAAAACTGG AATTTAGTTC TTCGGCAGAC TCTCTCAGTG ATTGGGCTGA TATAGATGAC 
TTTGATGTGC CAGCATCAGA CGCATTTGTT TCCCTGACCA GAAACCCTAC TACAAGAGTG 
AGCACTGCTA AGAAACTGAG TAGCAGTAAG AGAAACTTCT TTAAACCACC ACCTAGAGCA 
AGTGCAGTAA AGGCCGACTT GACTCCTTCC TCCCCCGAAT GCCTGCAAGT GGATTTAACG 
AAGGAACAGG AGGAAGAGGC GGACTGCCTG AGCAGGGATG TGATCTGCAT TGACACTGAC 
CCTGCTTCTG AGGAGCTCTT GGAGAAGGAT GTGCAGGAAG GCCTGTCATT GGACGCTCGC 
TTGGGGACTG AAAGAGTTGA CAGTGCAAAG AAGAGCCATG AAGACGAGGC TGCATTCCAG 
TCACCTCAGA ACGCTCTGTA CTTTGAACAC AATGACAATG ATTATGATAT AGATTTTGTT 
CCACCTTCTC CAGAAGAAAT CATTTCCACT GCTTCTTCCT CGTTGAAATG TTCCAGTATG 
CTAAAGGATC TCGATGACTC TGACAAAGAG AAGGACATTC TGAGTACCTC AAAAGATCTG 
TCAAAACCAG AGGAAATGAC CACACCGAAG TCTGATCTGG GAACCAATGA AGACTGTGAT 
GCCCAGCAGA CTAGCTTGCA GCAGCAGCTT ATCCGTGTGA TGGAGCACAT CTGTAAATTA 
GTTGATACTG TTCCTATTGA TGAACTGAAA GCTCTGACTT GTGGGGACAA ACTGCTTCAG 
CAGCGAGACA TAAGAAGGAA GCTCCTTGCT GAAGCAGGTT TTAATAGAAA CGATGTCAAT 
CTTCTGGGTT CTTTGTGGAG GCACAGGCCA GATTCGCTTG ATAACACTGT GCAGTGTGAC 
TCCTGTCCTG TGGGGCATCC TGGTAAAGAG TTAAACTCTC CATACCTTCC CTCACACTCC 
CTCTCCACTG AGGAATGCTT ACCCACCACT GCTTCAGGAA AGACAGGATT CTCAGCCACC 
CCGAAGAGTC TCTCTGAAAG GCCACCATTG AATTCCCACT TACAGAAGTC CTTTGTAAGT 
AGCAACTGGG CTGAAACACC AAGGACAGAA AGCAGGACGG AAAGCACTTA CTTCCCAGGG 
AGTGTTCTCA CAAGCACCAC TGTGAAGGAT GAGAGTAAAC ATGTTGCTTC AGGACGTGAC 
ATAGAGAGAG AAGTCCAGGC TTCCTACGAT ATTGATAATT TTAACATTGA TGACTTTGAT 
GATGATGATG ATGATGACTG GGAAAACATA ATGCACAATT TTTCAGTCAG CAAATCTTCC 
ACAGCTGCCT ACCCACCTAT CAAGGAAGGT GGCCCAGTTA AATCTCTATC AGAAAGGATT 
TCTTCAGCCA AGGCAAAGTT TCTTCCAGTG GCATCAACCG CTCAAAATAA AAACCTCTCA 
GAGTCAATTC AGAATTGCTC TGATAAGTTT GCACAAAATC TATCATCAAA AAATCCAAAA 
CATGAACATT TTCAAAGTCT TAATTTTCCT CATACAAAAG AAATGATGAA GATTTTCCGT 
AAGAAATTTG GCTTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCGCTG 
CTTGGTGAAG ACTGCTTTAT CCTGATGCCC ACTGGAGGTG GTAAAAGTTT GTGCTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGAGTCACT ATTGTCATTT CTCCCTTGAG ATCACTAATA 
GTGGACCAAG TCCAAAAGCT GACTTCCTTT GATATTCCAG CTACGTACCT GACAGGGGAT 
AAGACTGACT CCGAAGCTGC AAACATTTAC CTCCAGTTGT CAAAGAAAGA CCCGATCATC 
AAGCTTCTGT ATGTCACCCC AGAGAAGGTC TGTGCAAGTA ACAGGCTGAT TTCCACTCTG 
GAGAATCTGT ATGAGCGGAA GCTCTTGGCA CGATTTGTCA TTGATGAAGC ACACTGTGTG 
AGTCAGTGGG GCCATGATTT CCGTCAAGAT TACAAAAGGA TGAATATGCT TCGCCAGAGG 
TTCCCTTCTG TCCCAGTGAT GGCTCTCACG GCCACAGCGA ACCCCAGGGT ACAGAAGGAC 
ATCCTCACGC AGCTGAAGAT TCTCAGGCCT CAGGTGTTTA GCATGAGCTT TAACAGACAC 
AATCTGAAAT ACTATGTATT ACCAAAGAAG CCCAAGAAAG TGGCACTTGA TTGCCTAGAG 
TGGATCAGAA AGCATCACCC TTATGACTCG GGGATAATTT ATTGCCTCTC CAGGAAGGAA 
TGTGACACAA TGGCCGACAC CTTACAGAGA GAAGGTCTTG CTGCTCTGGC TTACCATGCA 
GGTCTTAGTG ATTCTGCCAG AGATGAGGTG CAGCACAAGT GGATCAACCA GGACAACTGC 
CAGGTTATCT GTGCAACCAT TGCATTTGGA ATGGGAATCG ACAAACCTGA CGTGCGATTT 
GTGGTTCATG CATCTCTTCC TAAATCTGTG GAGGGTTATT ACCAAGAATC CGGCCGAGCT 
GGGAGAGACG GGGAAATATC TCACTGCGTG CTTTTCTATA CCTATCATGA CGTGACCAGA 
CTGAAGAGAC TTATAATGAT GGAAAAAGAT GGAAACTATC ATACAAGGGA AACTCACGTC 
AATAATTTAT ATAGCATGGT ACATTACTGC GAAAACATAA CAGAATGCAG AAGAATACAG 
CTTTTAGCTT ACTTTGGTGA AAAAGGATTT AACCCTGATT TTTGTAAGAA ACATCCAGAT 
GTTTCTTGTG ACAATTGCTG TAAAACAAAG GATTATAAAA CAAAAGATGT GACTGATGAT 
GTGAAAAATA TTGTAAGATT TGTTCAAGAA CACAGTTCAT CACCAGGAAC AAGAAATATA 
GGACCTGCTG GAAGATTTAC TCTGAACATG CTAGTCGACA TTTTCTTGGG AAGTAAGAGT 
GCAAAAGTTC AGTCTGGAAT ATTTGGAAAG GGGTCTGCAT ATTCACGCCA CAATGCTGAA 
AGACTTTTTA AAAAGCTGAT TCTAGACAAA ATCTTGGATG AAGACTTATA TATCAATGCC 
AATGACCAAC CAATTGCCTA TGTGATGCTC GGAACAAAAG CCCACAGTGT GCTGAGCGGC 
CACTTGAAGG TGGACTTCAT GGAAACGGAA AATTCGAGCA GTGTTAAAAA ACAAAAAGCC 
GCAGTGGCCC ACGTGTCCCA GAGAGACGAG GCAGTTATGA AGTGTCTGGG AGAACTTGCA 
GACGTCTGCA AATTGCTGGG CAAAGTCTTT GGTGTCCATT ACTTCAATAT TTTCAATACA 
GCCACCCTCA AAAAGCTTGC AGAATCTTTA TCTTCTGATC CTGAGGTTTT GCTTCAGATT 
GATGGTGTTA CTGAAGACAA GCTGGAGAAA TATGGTGCAG AAATAATTCC AGTATTACAG 
AAGTACGCAG AGTGGACATT GCCAGCTGAA GGTGGGTCCC CAGGCAGCAG AGGCTCTAGG 
AGGAGCACCC CAGAGGAGAC GGAGGAGGAG GAAGCTGTAT CTTCTCACTA CTTTGCAAAT 
CAAACTAGAA ATGAAAGAAA GAGGAAGAAA GTGTCAGCCT CCCAAAAGCC CAAGAGAAGA 
AGAACTAGTT ACGGCGGCGG CTTCAGAGCA AAGGGGGGCT CTACTACATG CAGAAAACCA 
TCTTCTAAAA GTAAATTCTC TGGTGTAACT GGATCCCGCT CAGCCTCGTG TGCTTCTCAG 
GCAACATCAG CAGCCAGTAG AAAACTGGGG ATTATGGCTC CTCCAAAGCC TGTAAATAGA 
CCGTTCCTCA GGCCTGCGTA TGCCTTCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003753349.2
CDS	354..4586
Translation	MFGSPVGWIMAAVPLNNLQEQLQRHSARKLKNQFSVSKPKPSGFTFKKKTPEGDVSVVRTPVLSDKDVNMSEALSYTEPPLHKPKQQSNIDGFFKNSPGGQQSKETCSELSLPEVVQIPQDASCTTPKTPAVKKPHVAIFKKLEFSSSADSLSDWADIDDFDVPASDAFVSLTRNPTTRVSTAKKLSSSKRNFFKPPPRASAVKADLTPSSPECLQVDLTKEQEEEADCLSRDVICIDTDPASEELLEKDVQEGLSLDARLGTERVDSAKKSHEDEAAFQSPQNALYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKDILSTSKDLSKPEEMTTPKSDLGTNEDCDAQQTSLQQQLIRVMEHICKLVDTVPIDELKALTCGDKLLQQRDIRRKLLAEAGFNRNDVNLLGSLWRHRPDSLDNTVQCDSCPVGHPGKELNSPYLPSHSLSTEECLPTTASGKTGFSATPKSLSERPPLNSHLQKSFVSSNWAETPRTESRTESTYFPGSVLTSTTVKDESKHVASGRDIEREVQASYDIDNFNIDDFDDDDDDDWENIMHNFSVSKSSTAAYPPIKEGGPVKSLSERISSAKAKFLPVASTAQNKNLSESIQNCSDKFAQNLSSKNPKHEHFQSLNFPHTKEMMKIFRKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQRFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVALDCLEWIRKHHPYDSGIIYCLSRKECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVVHASLPKSVEGYYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTRETHVNNLYSMVHYCENITECRRIQLLAYFGEKGFNPDFCKKHPDVSCDNCCKTKDYKTKDVTDDVKNIVRFVQEHSSSPGTRNIGPAGRFTLNMLVDIFLGSKSAKVQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHLKVDFMETENSSSVKKQKAAVAHVSQRDEAVMKCLGELADVCKLLGKVFGVHYFNIFNTATLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEIIPVLQKYAEWTLPAEGGSPGSRGSRRSTPEETEEEEAVSSHYFANQTRNERKRKKVSASQKPKRRRTSYGGGFRAKGGSTTCRKPSSKSKFSGVTGSRSASCASQATSAASRKLGIMAPPKPVNRPFLRPAYAFS

Target ORF information:

RefSeq Version	XM_003753301.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003753301.4

ORF Insert Sequence:

ATGTTTGGAT CTCCCGTTGG TTGGATCATG GCTGCTGTTC CTCTGAACAA TCTACAAGAA 
CAACTACAGC GTCACTCGGC CAGAAAACTT AAAAATCAGT TCAGCGTTTC AAAACCAAAA 
CCTTCAGGTT TTACTTTTAA AAAGAAAACA CCAGAGGGTG ATGTGTCTGT GGTAAGAACA 
CCTGTGTTAA GCGATAAAGA TGTGAATATG TCTGAGGCCT TGTCATATAC TGAGCCTCCA 
CTCCACAAAC CAAAGCAGCA GTCAAACATC GATGGCTTCT TTAAAAATTC CCCTGGAGGG 
CAGCAAAGCA AGGAGACCTG CTCCGAACTG TCCCTGCCGG AGGTGGTACA GATTCCTCAG 
GACGCTTCTT GTACTACCCC AAAAACCCCA GCTGTGAAGA AACCCCACGT GGCCATTTTC 
AAAAAACTGG AATTTAGTTC TTCGGCAGAC TCTCTCAGTG ATTGGGCTGA TATAGATGAC 
TTTGATGTGC CAGCATCAGA CGCATTTGTT TCCCTGACCA GAAACCCTAC TACAAGAGTG 
AGCACTGCTA AGAAACTGAG TAGCAGTAAG AGAAACTTCT TTAAACCACC ACCTAGAGCA 
AGTGCAGTAA AGGCCGACTT GACTCCTTCC TCCCCCGAAT GCCTGCAAGT GGATTTAACG 
AAGGAACAGG AGGAAGAGGC GGACTGCCTG AGCAGGGATG TGATCTGCAT TGACACTGAC 
CCTGCTTCTG AGGAGCTCTT GGAGAAGGAT GTGCAGGAAG GCCTGTCATT GGACGCTCGC 
TTGGGGACTG AAAGAGTTGA CAGTGCAAAG AAGAGCCATG AAGACGAGGC TGCATTCCAG 
TCACCTCAGA ACGCTCTGTA CTTTGAACAC AATGACAATG ATTATGATAT AGATTTTGTT 
CCACCTTCTC CAGAAGAAAT CATTTCCACT GCTTCTTCCT CGTTGAAATG TTCCAGTATG 
CTAAAGGATC TCGATGACTC TGACAAAGAG AAGGACATTC TGAGTACCTC AAAAGATCTG 
TCAAAACCAG AGGAAATGAC CACACCGAAG TCTGATCTGG GAACCAATGA AGACTGTGAT 
GCCCAGCAGA CTAGCTTGCA GCAGCAGCTT ATCCGTGTGA TGGAGCACAT CTGTAAATTA 
GTTGATACTG TTCCTATTGA TGAACTGAAA GCTCTGACTT GTGGGGACAA ACTGCTTCAG 
CAGCGAGACA TAAGAAGGAA GCTCCTTGCT GAAGCAGGTT TTAATAGAAA CGATGTCAAT 
CTTCTGGGTT CTTTGTGGAG GCACAGGCCA GATTCGCTTG ATAACACTGT GCAGTGTGAC 
TCCTGTCCTG TGGGGCATCC TGGTAAAGAG TTAAACTCTC CATACCTTCC CTCACACTCC 
CTCTCCACTG AGGAATGCTT ACCCACCACT GCTTCAGGAA AGACAGGATT CTCAGCCACC 
CCGAAGAGTC TCTCTGAAAG GCCACCATTG AATTCCCACT TACAGAAGTC CTTTGTAAGT 
AGCAACTGGG CTGAAACACC AAGGACAGAA AGCAGGACGG AAAGCACTTA CTTCCCAGGG 
AGTGTTCTCA CAAGCACCAC TGTGAAGGAT GAGAGTAAAC ATGTTGCTTC AGGACGTGAC 
ATAGAGAGAG AAGTCCAGGC TTCCTACGAT ATTGATAATT TTAACATTGA TGACTTTGAT 
GATGATGATG ATGATGACTG GGAAAACATA ATGCACAATT TTTCAGTCAG CAAATCTTCC 
ACAGCTGCCT ACCCACCTAT CAAGGAAGGT GGCCCAGTTA AATCTCTATC AGAAAGGATT 
TCTTCAGCCA AGGCAAAGTT TCTTCCAGTG GCATCAACCG CTCAAAATAA AAACCTCTCA 
GAGTCAATTC AGAATTGCTC TGATAAGTTT GCACAAAATC TATCATCAAA AAATCCAAAA 
CATGAACATT TTCAAAGTCT TAATTTTCCT CATACAAAAG AAATGATGAA GATTTTCCGT 
AAGAAATTTG GCTTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCGCTG 
CTTGGTGAAG ACTGCTTTAT CCTGATGCCC ACTGGAGGTG GTAAAAGTTT GTGCTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGAGTCACT ATTGTCATTT CTCCCTTGAG ATCACTAATA 
GTGGACCAAG TCCAAAAGCT GACTTCCTTT GATATTCCAG CTACGTACCT GACAGGGGAT 
AAGACTGACT CCGAAGCTGC AAACATTTAC CTCCAGTTGT CAAAGAAAGA CCCGATCATC 
AAGCTTCTGT ATGTCACCCC AGAGAAGGTC TGTGCAAGTA ACAGGCTGAT TTCCACTCTG 
GAGAATCTGT ATGAGCGGAA GCTCTTGGCA CGATTTGTCA TTGATGAAGC ACACTGTGTG 
AGTCAGTGGG GCCATGATTT CCGTCAAGAT TACAAAAGGA TGAATATGCT TCGCCAGAGG 
TTCCCTTCTG TCCCAGTGAT GGCTCTCACG GCCACAGCGA ACCCCAGGGT ACAGAAGGAC 
ATCCTCACGC AGCTGAAGAT TCTCAGGCCT CAGGTGTTTA GCATGAGCTT TAACAGACAC 
AATCTGAAAT ACTATGTATT ACCAAAGAAG CCCAAGAAAG TGGCACTTGA TTGCCTAGAG 
TGGATCAGAA AGCATCACCC TTATGACTCG GGGATAATTT ATTGCCTCTC CAGGAAGGAA 
TGTGACACAA TGGCCGACAC CTTACAGAGA GAAGGTCTTG CTGCTCTGGC TTACCATGCA 
GGTCTTAGTG ATTCTGCCAG AGATGAGGTG CAGCACAAGT GGATCAACCA GGACAACTGC 
CAGGTTATCT GTGCAACCAT TGCATTTGGA ATGGGAATCG ACAAACCTGA CGTGCGATTT 
GTGGTTCATG CATCTCTTCC TAAATCTGTG GAGGGTTATT ACCAAGAATC CGGCCGAGCT 
GGGAGAGACG GGGAAATATC TCACTGCGTG CTTTTCTATA CCTATCATGA CGTGACCAGA 
CTGAAGAGAC TTATAATGAT GGAAAAAGAT GGAAACTATC ATACAAGGGA AACTCACGTC 
AATAATTTAT ATAGCATGGT ACATTACTGC GAAAACATAA CAGAATGCAG AAGAATACAG 
CTTTTAGCTT ACTTTGGTGA AAAAGGATTT AACCCTGATT TTTGTAAGAA ACATCCAGAT 
GTTTCTTGTG ACAATTGCTG TAAAACAAAG GATTATAAAA CAAAAGATGT GACTGATGAT 
GTGAAAAATA TTGTAAGATT TGTTCAAGAA CACAGTTCAT CACCAGGAAC AAGAAATATA 
GGACCTGCTG GAAGATTTAC TCTGAACATG CTAGTCGACA TTTTCTTGGG AAGTAAGAGT 
GCAAAAGTTC AGTCTGGAAT ATTTGGAAAG GGGTCTGCAT ATTCACGCCA CAATGCTGAA 
AGACTTTTTA AAAAGCTGAT TCTAGACAAA ATCTTGGATG AAGACTTATA TATCAATGCC 
AATGACCAAC CAATTGCCTA TGTGATGCTC GGAACAAAAG CCCACAGTGT GCTGAGCGGC 
CACTTGAAGG TGGACTTCAT GGAAACGGAA AATTCGAGCA GTGTTAAAAA ACAAAAAGCC 
GCAGTGGCCC ACGTGTCCCA GAGAGACGAG GCAGTTATGA AGTGTCTGGG AGAACTTGCA 
GACGTCTGCA AATTGCTGGG CAAAGTCTTT GGTGTCCATT ACTTCAATAT TTTCAATACA 
GCCACCCTCA AAAAGCTTGC AGAATCTTTA TCTTCTGATC CTGAGGTTTT GCTTCAGATT 
GATGGTGTTA CTGAAGACAA GCTGGAGAAA TATGGTGCAG AAATAATTCC AGTATTACAG 
AAGTACGCAG AGTGGACATT GCCAGCTGAA GGTGGGTCCC CAGGCAGCAG AGGCTCTAGG 
AGGAGCACCC CAGAGGAGAC GGAGGAGGAG GAAGCTGTAT CTTCTCACTA CTTTGCAAAT 
CAAACTAGAA ATGAAAGAAA GAGGAAGAAA GTGTCAGCCT CCCAAAAGCC CAAGAGAAGA 
AGAACTAGTT ACGGCGGCGG CTTCAGAGCA AAGGGGGGCT CTACTACATG CAGAAAACCA 
TCTTCTAAAA GTAAATTCTC TGGTGTAACT GGATCCCGCT CAGCCTCGTG TGCTTCTCAG 
GCAACATCAG CAGCCAGTAG AAAACTGGGG ATTATGGCTC CTCCAAAGCC TGTAAATAGA 
CCGTTCCTCA GGCCTGCGTA TGCCTTCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blm ( XM_006229458.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229520.1 Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X1, mRNA.

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CloneID	ORa22955
Clone ID Related Accession (Same CDS sequence)	XM_006229458.3
Accession Version	XM_006229458.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Bloom syndrome protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229458.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAT CTCCCGTTGG TTGGATCATG GCTGCTGTTC CTCTGAACAA TCTACAAGAA 
CAACTACAGC GTCACTCGGC CAGAAAACTT AAAAATCAGT TCAGCATTTC CAAACCAAAA 
CCTTCAGGTT TTACTTTTAA AAAGAAAACA CCAGAGGGTG ATGTGTCTGT GGTAAGAACA 
CCTGTGTTAA GCGATAAAGA TGTGAATATG TCTGAGGCCT TGTCATATAC TGAGCCTCCA 
CTCCACAAAC CAAAGCAGCA GTCAAACATC GATGGCTTCT TTAAAAATTC CCCTGGAGGG 
CAGCAAAGCA AGGAGACCTG CTCCGAACTG TCCCTGCCGG AGGTGGTACA GATTCCTCAG 
GACGCTTCTT GTACTACCCC AAAAACCCCA GCTGTGAAGA AACCCCACGT GGCCATTTTC 
AAAAAACTGG AATTTAGTTC TTCGGCAGAC TCTCTCAGTG ATTGGGCTGA TATAGATGAC 
TTTGATGTGC CAGCATCAGA CGCATTTGTT TCCCTGACCA GAAACCCTAC TACAAGAGTG 
AGCACTGCTA AGAAACTGAG TAGCAGTAAG AGAAACTTCT TTAAACCACC ACCTAGAGCA 
AGTGCAGTAA AGGCCGACTT GACTCCTTCC TCCCCCGAAT GCCTGCAAGT GGATTTAACG 
AAGGAACAGG AGGAAGAGGC GGACTGCCTG AGCAGGGATG TGATCTGCAT TGACACTGAC 
CCTGCTTCTG AGGAGCTCTT GGAGAAGGAT GTGCAGGAAG GCCTGTCATT GGACGCTCGC 
TTGGGGACTG AAAGAGGTAT TGACAGTGCA AAGAAGAGCC ATGAAGACAA GGCTGCATTC 
CAGTCACCTC AGAACGCTCT GTACTTTGAA CACAATGACA ATGATTATGA TATAGATTTT 
GTTCCACCTT CTCCAGAAGA AATCATTTCC ACTGCTTCTT CCTCGTTGAA ATGTTCCAGT 
ATGCTAAAGG ATCTCGATGA CTCTGACAAA GAGAAGGACA TTCTGAGTAC CTCAAAAGAT 
CTGTCAAAAC CAGAGGAAAT GACCACACCG AAGTCTGATC TGGGAACCAA TGAAGACTGT 
GATGCCCAGC AGACTAGCTT GCAGCAGCAG CTTATCCGTG TGATGGAGCA CATCTGTAAA 
TTAGTTGATA CTGTTCCTAT TGATGAACTG AAAGCTCTGA CTTGTGGGGA CAAACTGCTT 
CAGCAGCGAG ACATAAGAAG GAAGCTCCTT GCTGAAGCAG GTTTTAATAG AAACGATGTC 
AATCTTCTGG GTTCTTTGTG GAGGCACAGG CCAGATTCGC TTGATAACAC TGTGCAGTGT 
GACTCCTGTC CTGTGGGGCA TCCTGGTAAA GAGTTAAACT CTCCATACCT TCCCTCACAC 
TCCCTCTCCA CTGAGGAATG CTTACCCACC ACTGCTTCAG GAAAGACAGG ATTCTCAGCC 
ACCCCGAAGA GTCTCTCTGA AAGGCCACCA TTGAATTCCC ACTTACAGAA GTCCTTTGTA 
AGTAGCAACT GGGCTGAAAC ACCAAGGACA GAAAGCAGGA CGGAAAGCAC TTACTTCCCA 
GGGAGTGTTC TCACAAGCAC CACTGTGAAG GATGAGAGTA AACATGTTGC TTCAGGACGT 
GACATAGAGA GAGAAGTCCA GGCTTCCTAC GATATTGATA ATTTTAACAT TGATGACTTT 
GATGATGATG ATGATGATGA CTGGGAAAAC ATAATGCACA ATTTTTCAGT CAGCAAATCT 
TCCACAGCTG CCTACCCACC TATCAAGGAA GGTGGCCCAG TTAAATCTCT ATCAGAAAGG 
ATTTCTTCAG CCAAGGCAAA GTTTCTTCCA GTGGCATCAA CCGCTCAAAA TAAAAACCTC 
TCAGAGTCAA TTCAGAATTG CTCTGATAAG TTTGCACAAA ATCTATCATC AAAAAATCCA 
AAACATGAAC ATTTTCAAAG TCTTAATTTT CCTCATACAA AAGAAATGAT GAAGATTTTC 
CGTAAGAAAT TTGGCTTGCA TAATTTTAGA ACTAATCAGC TAGAGGCGAT CAATGCTGCG 
CTGCTTGGTG AAGACTGCTT TATCCTGATG CCCACTGGAG GTGGTAAAAG TTTGTGCTAC 
CAGCTCCCTG CCTGTGTTTC TCCTGGAGTC ACTATTGTCA TTTCTCCCTT GAGATCACTA 
ATAGTGGACC AAGTCCAAAA GCTGACTTCC TTTGATATTC CAGCTACGTA CCTGACAGGG 
GATAAGACTG ACTCCGAAGC TGCAAACATT TACCTCCAGT TGTCAAAGAA AGACCCGATC 
ATCAAGCTTC TGTATGTCAC CCCAGAGAAG GTCTGTGCAA GTAACAGGCT GATTTCCACT 
CTGGAGAATC TGTATGAGCG AAAGCTCTTG GCACGATTTG TCATTGATGA AGCACACTGT 
GTGAGTCAGT GGGGCCATGA TTTCCGTCAA GATTACAAAA GGATGAATAT GCTTCGCCAG 
AGGTTCCCTT CTGTCCCAGT GATGGCTCTC ACGGCCACAG CGAACCCCAG GGTACAGAAG 
GACATCCTCA CGCAGCTGAA GATTCTCAGG CCTCAGGTGT TTAGCATGAG CTTTAACAGA 
CACAATCTGA AATACTATGT ATTACCAAAG AAGCCCAAGA AAGTGGCACT TGATTGCCTA 
GAGTGGATCA GAAAGCATCA CCCTTATGAC TCGGGGATAA TTTATTGCCT CTCCAGGAAG 
GAATGTGACA CAATGGCCGA CACCTTACAG AGAGAAGGTC TTGCTGCTCT GGCTTACCAT 
GCAGGTCTTA GTGATTCTGC CAGAGATGAG GTGCAGCACA AGTGGATCAA CCAGGACAAC 
TGCCAGGTTA TCTGTGCAAC CATTGCATTT GGAATGGGAA TCGACAAACC TGACGTGCGA 
TTTGTGGTTC ATGCATCTCT TCCTAAATCT GTGGAGGGTT ATTACCAAGA ATCCGGCCGA 
GCTGGGAGAG ACGGGGAAAT ATCTCACTGC GTGCTTTTCT ATACCTATCA TGACGTGACC 
AGACTGAAGA GACTTATAAT GATGGAAAAA GATGGAAACT ATCATACAAG GGAAACTCAC 
GTCAATAATT TATATAGCAT GGTACATTAC TGCGAAAACA TAACAGAATG CAGAAGAATA 
CAGCTTTTAG CTTACTTTGG TGAAAAAGGA TTTAACCCTG ATTTTTGTAA GAAACATCCA 
GATGTTTCTT GTGACAATTG CTGTAAAACA AAGGATTATA AAACAAAAGA TGTGACTGAT 
GATGTGAAAA ATATTGTAAG ATTTGTTCAA GAACACAGTT CATCACCAGG AACAAGAAAT 
ATAGGACCTG CTGGAAGATT TACTCTGAAC ATGCTAGTCG ACATTTTCTT GGGAAGTAAG 
AGTGCAAAAG TTCAGTCTGG AATATTTGGA AAGGGGTCTG CATATTCACG CCACAATGCT 
GAAAGACTTT TTAAAAAGCT GATTCTAGAC AAAATCTTGG ATGAAGACTT ATATATCAAT 
GCCAATGACC AACCAATTGC CTATGTGATG CTCGGAACAA AAGCCCACAG TGTGCTGAGC 
GGCCACTTGA AGGTGGACTT CATGGAAACG GAAAATTCGA GCAGTGTTAA AAAACAAAAA 
GCCGCAGTGG CCCACGTGTC CCAGAGAGAC GAGGCAGTTA TGAAGTGTCT GGGAGAACTT 
GCAGACGTCT GCAAATTGCT GGGCAAAGTC TTTGGTGTCC ATTACTTCAA TATTTTCAAT 
ACAGCCACCC TCAAAAAGCT TGCAGAATCT TTATCTTCTG ATCCTGAGGT TTTGCTTCAG 
ATTGATGGTG TTACTGAAGA CAAGCTGGAG AAATATGGTG CAGAAATAAT TCCAGTATTA 
CAGAAGTACG CAGAGTGGAC ATTGCCAGCT GAAGGTGGGT CCCCAGGCAG CAGAGGCTCT 
AGGAGGAGCA CCCCAGAGGA GACGGAGGAG GAGGAAGCTG TATCTTCTCA CTACTTTGCA 
AATCAAACTA GAAATGAAAG AAAGAGGAAG AAAGTGTCAG CCTCCCAAAA GCCCAAGAGA 
AGAAGAACTA GTTACGGCGG CGGCTTCAGA GCAAAGGGGG GCTCTACTAC ATGCAGAAAA 
CCATCTTCTA AAAGTAAATT CTCTGGTGTA ACTGGATCCC GCTCAGCCTC GTGTGCTTCT 
CAGGCAACAT CAGCAGCCAG TAGAAAACTG GGGATTATGG CTCCTCCAAA GCCTGTAAAT 
AGACCGTTCC TCAGGCCTGC GTATGCCTTC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229520.1
CDS	354..4589
Translation	MFGSPVGWIMAAVPLNNLQEQLQRHSARKLKNQFSISKPKPSGFTFKKKTPEGDVSVVRTPVLSDKDVNMSEALSYTEPPLHKPKQQSNIDGFFKNSPGGQQSKETCSELSLPEVVQIPQDASCTTPKTPAVKKPHVAIFKKLEFSSSADSLSDWADIDDFDVPASDAFVSLTRNPTTRVSTAKKLSSSKRNFFKPPPRASAVKADLTPSSPECLQVDLTKEQEEEADCLSRDVICIDTDPASEELLEKDVQEGLSLDARLGTERGIDSAKKSHEDKAAFQSPQNALYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKDILSTSKDLSKPEEMTTPKSDLGTNEDCDAQQTSLQQQLIRVMEHICKLVDTVPIDELKALTCGDKLLQQRDIRRKLLAEAGFNRNDVNLLGSLWRHRPDSLDNTVQCDSCPVGHPGKELNSPYLPSHSLSTEECLPTTASGKTGFSATPKSLSERPPLNSHLQKSFVSSNWAETPRTESRTESTYFPGSVLTSTTVKDESKHVASGRDIEREVQASYDIDNFNIDDFDDDDDDDWENIMHNFSVSKSSTAAYPPIKEGGPVKSLSERISSAKAKFLPVASTAQNKNLSESIQNCSDKFAQNLSSKNPKHEHFQSLNFPHTKEMMKIFRKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQRFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVALDCLEWIRKHHPYDSGIIYCLSRKECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVVHASLPKSVEGYYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTRETHVNNLYSMVHYCENITECRRIQLLAYFGEKGFNPDFCKKHPDVSCDNCCKTKDYKTKDVTDDVKNIVRFVQEHSSSPGTRNIGPAGRFTLNMLVDIFLGSKSAKVQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHLKVDFMETENSSSVKKQKAAVAHVSQRDEAVMKCLGELADVCKLLGKVFGVHYFNIFNTATLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEIIPVLQKYAEWTLPAEGGSPGSRGSRRSTPEETEEEEAVSSHYFANQTRNERKRKKVSASQKPKRRRTSYGGGFRAKGGSTTCRKPSSKSKFSGVTGSRSASCASQATSAASRKLGIMAPPKPVNRPFLRPAYAFS

Target ORF information:

RefSeq Version	XM_006229458.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229458.3

ORF Insert Sequence:

ATGTTTGGAT CTCCCGTTGG TTGGATCATG GCTGCTGTTC CTCTGAACAA TCTACAAGAA 
CAACTACAGC GTCACTCGGC CAGAAAACTT AAAAATCAGT TCAGCATTTC CAAACCAAAA 
CCTTCAGGTT TTACTTTTAA AAAGAAAACA CCAGAGGGTG ATGTGTCTGT GGTAAGAACA 
CCTGTGTTAA GCGATAAAGA TGTGAATATG TCTGAGGCCT TGTCATATAC TGAGCCTCCA 
CTCCACAAAC CAAAGCAGCA GTCAAACATC GATGGCTTCT TTAAAAATTC CCCTGGAGGG 
CAGCAAAGCA AGGAGACCTG CTCCGAACTG TCCCTGCCGG AGGTGGTACA GATTCCTCAG 
GACGCTTCTT GTACTACCCC AAAAACCCCA GCTGTGAAGA AACCCCACGT GGCCATTTTC 
AAAAAACTGG AATTTAGTTC TTCGGCAGAC TCTCTCAGTG ATTGGGCTGA TATAGATGAC 
TTTGATGTGC CAGCATCAGA CGCATTTGTT TCCCTGACCA GAAACCCTAC TACAAGAGTG 
AGCACTGCTA AGAAACTGAG TAGCAGTAAG AGAAACTTCT TTAAACCACC ACCTAGAGCA 
AGTGCAGTAA AGGCCGACTT GACTCCTTCC TCCCCCGAAT GCCTGCAAGT GGATTTAACG 
AAGGAACAGG AGGAAGAGGC GGACTGCCTG AGCAGGGATG TGATCTGCAT TGACACTGAC 
CCTGCTTCTG AGGAGCTCTT GGAGAAGGAT GTGCAGGAAG GCCTGTCATT GGACGCTCGC 
TTGGGGACTG AAAGAGGTAT TGACAGTGCA AAGAAGAGCC ATGAAGACAA GGCTGCATTC 
CAGTCACCTC AGAACGCTCT GTACTTTGAA CACAATGACA ATGATTATGA TATAGATTTT 
GTTCCACCTT CTCCAGAAGA AATCATTTCC ACTGCTTCTT CCTCGTTGAA ATGTTCCAGT 
ATGCTAAAGG ATCTCGATGA CTCTGACAAA GAGAAGGACA TTCTGAGTAC CTCAAAAGAT 
CTGTCAAAAC CAGAGGAAAT GACCACACCG AAGTCTGATC TGGGAACCAA TGAAGACTGT 
GATGCCCAGC AGACTAGCTT GCAGCAGCAG CTTATCCGTG TGATGGAGCA CATCTGTAAA 
TTAGTTGATA CTGTTCCTAT TGATGAACTG AAAGCTCTGA CTTGTGGGGA CAAACTGCTT 
CAGCAGCGAG ACATAAGAAG GAAGCTCCTT GCTGAAGCAG GTTTTAATAG AAACGATGTC 
AATCTTCTGG GTTCTTTGTG GAGGCACAGG CCAGATTCGC TTGATAACAC TGTGCAGTGT 
GACTCCTGTC CTGTGGGGCA TCCTGGTAAA GAGTTAAACT CTCCATACCT TCCCTCACAC 
TCCCTCTCCA CTGAGGAATG CTTACCCACC ACTGCTTCAG GAAAGACAGG ATTCTCAGCC 
ACCCCGAAGA GTCTCTCTGA AAGGCCACCA TTGAATTCCC ACTTACAGAA GTCCTTTGTA 
AGTAGCAACT GGGCTGAAAC ACCAAGGACA GAAAGCAGGA CGGAAAGCAC TTACTTCCCA 
GGGAGTGTTC TCACAAGCAC CACTGTGAAG GATGAGAGTA AACATGTTGC TTCAGGACGT 
GACATAGAGA GAGAAGTCCA GGCTTCCTAC GATATTGATA ATTTTAACAT TGATGACTTT 
GATGATGATG ATGATGATGA CTGGGAAAAC ATAATGCACA ATTTTTCAGT CAGCAAATCT 
TCCACAGCTG CCTACCCACC TATCAAGGAA GGTGGCCCAG TTAAATCTCT ATCAGAAAGG 
ATTTCTTCAG CCAAGGCAAA GTTTCTTCCA GTGGCATCAA CCGCTCAAAA TAAAAACCTC 
TCAGAGTCAA TTCAGAATTG CTCTGATAAG TTTGCACAAA ATCTATCATC AAAAAATCCA 
AAACATGAAC ATTTTCAAAG TCTTAATTTT CCTCATACAA AAGAAATGAT GAAGATTTTC 
CGTAAGAAAT TTGGCTTGCA TAATTTTAGA ACTAATCAGC TAGAGGCGAT CAATGCTGCG 
CTGCTTGGTG AAGACTGCTT TATCCTGATG CCCACTGGAG GTGGTAAAAG TTTGTGCTAC 
CAGCTCCCTG CCTGTGTTTC TCCTGGAGTC ACTATTGTCA TTTCTCCCTT GAGATCACTA 
ATAGTGGACC AAGTCCAAAA GCTGACTTCC TTTGATATTC CAGCTACGTA CCTGACAGGG 
GATAAGACTG ACTCCGAAGC TGCAAACATT TACCTCCAGT TGTCAAAGAA AGACCCGATC 
ATCAAGCTTC TGTATGTCAC CCCAGAGAAG GTCTGTGCAA GTAACAGGCT GATTTCCACT 
CTGGAGAATC TGTATGAGCG AAAGCTCTTG GCACGATTTG TCATTGATGA AGCACACTGT 
GTGAGTCAGT GGGGCCATGA TTTCCGTCAA GATTACAAAA GGATGAATAT GCTTCGCCAG 
AGGTTCCCTT CTGTCCCAGT GATGGCTCTC ACGGCCACAG CGAACCCCAG GGTACAGAAG 
GACATCCTCA CGCAGCTGAA GATTCTCAGG CCTCAGGTGT TTAGCATGAG CTTTAACAGA 
CACAATCTGA AATACTATGT ATTACCAAAG AAGCCCAAGA AAGTGGCACT TGATTGCCTA 
GAGTGGATCA GAAAGCATCA CCCTTATGAC TCGGGGATAA TTTATTGCCT CTCCAGGAAG 
GAATGTGACA CAATGGCCGA CACCTTACAG AGAGAAGGTC TTGCTGCTCT GGCTTACCAT 
GCAGGTCTTA GTGATTCTGC CAGAGATGAG GTGCAGCACA AGTGGATCAA CCAGGACAAC 
TGCCAGGTTA TCTGTGCAAC CATTGCATTT GGAATGGGAA TCGACAAACC TGACGTGCGA 
TTTGTGGTTC ATGCATCTCT TCCTAAATCT GTGGAGGGTT ATTACCAAGA ATCCGGCCGA 
GCTGGGAGAG ACGGGGAAAT ATCTCACTGC GTGCTTTTCT ATACCTATCA TGACGTGACC 
AGACTGAAGA GACTTATAAT GATGGAAAAA GATGGAAACT ATCATACAAG GGAAACTCAC 
GTCAATAATT TATATAGCAT GGTACATTAC TGCGAAAACA TAACAGAATG CAGAAGAATA 
CAGCTTTTAG CTTACTTTGG TGAAAAAGGA TTTAACCCTG ATTTTTGTAA GAAACATCCA 
GATGTTTCTT GTGACAATTG CTGTAAAACA AAGGATTATA AAACAAAAGA TGTGACTGAT 
GATGTGAAAA ATATTGTAAG ATTTGTTCAA GAACACAGTT CATCACCAGG AACAAGAAAT 
ATAGGACCTG CTGGAAGATT TACTCTGAAC ATGCTAGTCG ACATTTTCTT GGGAAGTAAG 
AGTGCAAAAG TTCAGTCTGG AATATTTGGA AAGGGGTCTG CATATTCACG CCACAATGCT 
GAAAGACTTT TTAAAAAGCT GATTCTAGAC AAAATCTTGG ATGAAGACTT ATATATCAAT 
GCCAATGACC AACCAATTGC CTATGTGATG CTCGGAACAA AAGCCCACAG TGTGCTGAGC 
GGCCACTTGA AGGTGGACTT CATGGAAACG GAAAATTCGA GCAGTGTTAA AAAACAAAAA 
GCCGCAGTGG CCCACGTGTC CCAGAGAGAC GAGGCAGTTA TGAAGTGTCT GGGAGAACTT 
GCAGACGTCT GCAAATTGCT GGGCAAAGTC TTTGGTGTCC ATTACTTCAA TATTTTCAAT 
ACAGCCACCC TCAAAAAGCT TGCAGAATCT TTATCTTCTG ATCCTGAGGT TTTGCTTCAG 
ATTGATGGTG TTACTGAAGA CAAGCTGGAG AAATATGGTG CAGAAATAAT TCCAGTATTA 
CAGAAGTACG CAGAGTGGAC ATTGCCAGCT GAAGGTGGGT CCCCAGGCAG CAGAGGCTCT 
AGGAGGAGCA CCCCAGAGGA GACGGAGGAG GAGGAAGCTG TATCTTCTCA CTACTTTGCA 
AATCAAACTA GAAATGAAAG AAAGAGGAAG AAAGTGTCAG CCTCCCAAAA GCCCAAGAGA 
AGAAGAACTA GTTACGGCGG CGGCTTCAGA GCAAAGGGGG GCTCTACTAC ATGCAGAAAA 
CCATCTTCTA AAAGTAAATT CTCTGGTGTA ACTGGATCCC GCTCAGCCTC GTGTGCTTCT 
CAGGCAACAT CAGCAGCCAG TAGAAAACTG GGGATTATGG CTCCTCCAAA GCCTGTAAAT 
AGACCGTTCC TCAGGCCTGC GTATGCCTTC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blm ( XM_003748888.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_003748936.2 Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa22956
Clone ID Related Accession (Same CDS sequence)	XM_003748888.4
Accession Version	XM_003748888.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Bloom syndrome protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_003748888.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAT CTCCCGTTGG TTGGATCATG GCTGCTGTTC CTCTGAACAA TCTACAAGAA 
CAACTACAGC GTCACTCGGC CAGAAAACTT AAAAATCAGT TCAGCATTTC CAAACCAAAA 
CCTTCAGGTT TTACTTTTAA AAAGAAAACA CCAGAGGGTG ATGTGTCTGT GGTAAGAACA 
CCTGTGTTAA GCGATAAAGA TGTGAATATG TCTGAGGCCT TGTCATATAC TGAGCCTCCA 
CTCCACAAAC CAAAGCAGCA GTCAAACATC GATGGCTTCT TTAAAAATTC CCCTGGAGGG 
CAGCAAAGCA AGGAGACCTG CTCCGAACTG TCCCTGCCGG AGGTGGTACA GATTCCTCAG 
GACGCTTCTT GTACTACCCC AAAAACCCCA GCTGTGAAGA AACCCCACGT GGCCATTTTC 
AAAAAACTGG AATTTAGTTC TTCGGCAGAC TCTCTCAGTG ATTGGGCTGA TATAGATGAC 
TTTGATGTGC CAGCATCAGA CGCATTTGTT TCCCTGACCA GAAACCCTAC TACAAGAGTG 
AGCACTGCTA AGAAACTGAG TAGCAGTAAG AGAAACTTCT TTAAACCACC ACCTAGAGCA 
AGTGCAGTAA AGGCCGACTT GACTCCTTCC TCCCCCGAAT GCCTGCAAGT GGATTTAACG 
AAGGAACAGG AGGAAGAGGC GGACTGCCTG AGCAGGGATG TGATCTGCAT TGACACTGAC 
CCTGCTTCTG AGGAGCTCTT GGAGAAGGAT GTGCAGGAAG GCCTGTCATT GGACGCTCGC 
TTGGGGACTG AAAGAGTTGA CAGTGCAAAG AAGAGCCATG AAGACAAGGC TGCATTCCAG 
TCACCTCAGA ACGCTCTGTA CTTTGAACAC AATGACAATG ATTATGATAT AGATTTTGTT 
CCACCTTCTC CAGAAGAAAT CATTTCCACT GCTTCTTCCT CGTTGAAATG TTCCAGTATG 
CTAAAGGATC TCGATGACTC TGACAAAGAG AAGGACATTC TGAGTACCTC AAAAGATCTG 
TCAAAACCAG AGGAAATGAC CACACCGAAG TCTGATCTGG GAACCAATGA AGACTGTGAT 
GCCCAGCAGA CTAGCTTGCA GCAGCAGCTT ATCCGTGTGA TGGAGCACAT CTGTAAATTA 
GTTGATACTG TTCCTATTGA TGAACTGAAA GCTCTGACTT GTGGGGACAA ACTGCTTCAG 
CAGCGAGACA TAAGAAGGAA GCTCCTTGCT GAAGCAGGTT TTAATAGAAA CGATGTCAAT 
CTTCTGGGTT CTTTGTGGAG GCACAGGCCA GATTCGCTTG ATAACACTGT GCAGTGTGAC 
TCCTGTCCTG TGGGGCATCC TGGTAAAGAG TTAAACTCTC CATACCTTCC CTCACACTCC 
CTCTCCACTG AGGAATGCTT ACCCACCACT GCTTCAGGAA AGACAGGATT CTCAGCCACC 
CCGAAGAGTC TCTCTGAAAG GCCACCATTG AATTCCCACT TACAGAAGTC CTTTGTAAGT 
AGCAACTGGG CTGAAACACC AAGGACAGAA AGCAGGACGG AAAGCACTTA CTTCCCAGGG 
AGTGTTCTCA CAAGCACCAC TGTGAAGGAT GAGAGTAAAC ATGTTGCTTC AGGACGTGAC 
ATAGAGAGAG AAGTCCAGGC TTCCTACGAT ATTGATAATT TTAACATTGA TGACTTTGAT 
GATGATGATG ATGATGACTG GGAAAACATA ATGCACAATT TTTCAGTCAG CAAATCTTCC 
ACAGCTGCCT ACCCACCTAT CAAGGAAGGT GGCCCAGTTA AATCTCTATC AGAAAGGATT 
TCTTCAGCCA AGGCAAAGTT TCTTCCAGTG GCATCAACCG CTCAAAATAA AAACCTCTCA 
GAGTCAATTC AGAATTGCTC TGATAAGTTT GCACAAAATC TATCATCAAA AAATCCAAAA 
CATGAACATT TTCAAAGTCT TAATTTTCCT CATACAAAAG AAATGATGAA GATTTTCCGT 
AAGAAATTTG GCTTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCGCTG 
CTTGGTGAAG ACTGCTTTAT CCTGATGCCC ACTGGAGGTG GTAAAAGTTT GTGCTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGAGTCACT ATTGTCATTT CTCCCTTGAG ATCACTAATA 
GTGGACCAAG TCCAAAAGCT GACTTCCTTT GATATTCCAG CTACGTACCT GACAGGGGAT 
AAGACTGACT CCGAAGCTGC AAACATTTAC CTCCAGTTGT CAAAGAAAGA CCCGATCATC 
AAGCTTCTGT ATGTCACCCC AGAGAAGGTC TGTGCAAGTA ACAGGCTGAT TTCCACTCTG 
GAGAATCTGT ATGAGCGAAA GCTCTTGGCA CGATTTGTCA TTGATGAAGC ACACTGTGTG 
AGTCAGTGGG GCCATGATTT CCGTCAAGAT TACAAAAGGA TGAATATGCT TCGCCAGAGG 
TTCCCTTCTG TCCCAGTGAT GGCTCTCACG GCCACAGCGA ACCCCAGGGT ACAGAAGGAC 
ATCCTCACGC AGCTGAAGAT TCTCAGGCCT CAGGTGTTTA GCATGAGCTT TAACAGACAC 
AATCTGAAAT ACTATGTATT ACCAAAGAAG CCCAAGAAAG TGGCACTTGA TTGCCTAGAG 
TGGATCAGAA AGCATCACCC TTATGACTCG GGGATAATTT ATTGCCTCTC CAGGAAGGAA 
TGTGACACAA TGGCCGACAC CTTACAGAGA GAAGGTCTTG CTGCTCTGGC TTACCATGCA 
GGTCTTAGTG ATTCTGCCAG AGATGAGGTG CAGCACAAGT GGATCAACCA GGACAACTGC 
CAGGTTATCT GTGCAACCAT TGCATTTGGA ATGGGAATCG ACAAACCTGA CGTGCGATTT 
GTGGTTCATG CATCTCTTCC TAAATCTGTG GAGGGTTATT ACCAAGAATC CGGCCGAGCT 
GGGAGAGACG GGGAAATATC TCACTGCGTG CTTTTCTATA CCTATCATGA CGTGACCAGA 
CTGAAGAGAC TTATAATGAT GGAAAAAGAT GGAAACTATC ATACAAGGGA AACTCACGTC 
AATAATTTAT ATAGCATGGT ACATTACTGC GAAAACATAA CAGAATGCAG AAGAATACAG 
CTTTTAGCTT ACTTTGGTGA AAAAGGATTT AACCCTGATT TTTGTAAGAA ACATCCAGAT 
GTTTCTTGTG ACAATTGCTG TAAAACAAAG GATTATAAAA CAAAAGATGT GACTGATGAT 
GTGAAAAATA TTGTAAGATT TGTTCAAGAA CACAGTTCAT CACCAGGAAC AAGAAATATA 
GGACCTGCTG GAAGATTTAC TCTGAACATG CTAGTCGACA TTTTCTTGGG AAGTAAGAGT 
GCAAAAGTTC AGTCTGGAAT ATTTGGAAAG GGGTCTGCAT ATTCACGCCA CAATGCTGAA 
AGACTTTTTA AAAAGCTGAT TCTAGACAAA ATCTTGGATG AAGACTTATA TATCAATGCC 
AATGACCAAC CAATTGCCTA TGTGATGCTC GGAACAAAAG CCCACAGTGT GCTGAGCGGC 
CACTTGAAGG TGGACTTCAT GGAAACGGAA AATTCGAGCA GTGTTAAAAA ACAAAAAGCC 
GCAGTGGCCC ACGTGTCCCA GAGAGACGAG GCAGTTATGA AGTGTCTGGG AGAACTTGCA 
GACGTCTGCA AATTGCTGGG CAAAGTCTTT GGTGTCCATT ACTTCAATAT TTTCAATACA 
GCCACCCTCA AAAAGCTTGC AGAATCTTTA TCTTCTGATC CTGAGGTTTT GCTTCAGATT 
GATGGTGTTA CTGAAGACAA GCTGGAGAAA TATGGTGCAG AAATAATTCC AGTATTACAG 
AAGTACGCAG AGTGGACATT GCCAGCTGAA GGTGGGTCCC CAGGCAGCAG AGGCTCTAGG 
AGGAGCACCC CAGAGGAGAC GGAGGAGGAG GAAGCTGTAT CTTCTCACTA CTTTGCAAAT 
CAAACTAGAA ATGAAAGAAA GAGGAAGAAA GTGTCAGCCT CCCAAAAGCC CAAGAGAAGA 
AGAACTAGTT ACGGCGGCGG CTTCAGAGCA AAGGGGGGCT CTACTACATG CAGAAAACCA 
TCTTCTAAAA GTAAATTCTC TGGTGTAACT GGATCCCGCT CAGCCTCGTG TGCTTCTCAG 
GCAACATCAG CAGCCAGTAG AAAACTGGGG ATTATGGCTC CTCCAAAGCC TGTAAATAGA 
CCGTTCCTCA GGCCTGCGTA TGCCTTCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003748936.2
CDS	354..4586
Translation	MFGSPVGWIMAAVPLNNLQEQLQRHSARKLKNQFSISKPKPSGFTFKKKTPEGDVSVVRTPVLSDKDVNMSEALSYTEPPLHKPKQQSNIDGFFKNSPGGQQSKETCSELSLPEVVQIPQDASCTTPKTPAVKKPHVAIFKKLEFSSSADSLSDWADIDDFDVPASDAFVSLTRNPTTRVSTAKKLSSSKRNFFKPPPRASAVKADLTPSSPECLQVDLTKEQEEEADCLSRDVICIDTDPASEELLEKDVQEGLSLDARLGTERVDSAKKSHEDKAAFQSPQNALYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKDILSTSKDLSKPEEMTTPKSDLGTNEDCDAQQTSLQQQLIRVMEHICKLVDTVPIDELKALTCGDKLLQQRDIRRKLLAEAGFNRNDVNLLGSLWRHRPDSLDNTVQCDSCPVGHPGKELNSPYLPSHSLSTEECLPTTASGKTGFSATPKSLSERPPLNSHLQKSFVSSNWAETPRTESRTESTYFPGSVLTSTTVKDESKHVASGRDIEREVQASYDIDNFNIDDFDDDDDDDWENIMHNFSVSKSSTAAYPPIKEGGPVKSLSERISSAKAKFLPVASTAQNKNLSESIQNCSDKFAQNLSSKNPKHEHFQSLNFPHTKEMMKIFRKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQRFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVALDCLEWIRKHHPYDSGIIYCLSRKECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVVHASLPKSVEGYYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTRETHVNNLYSMVHYCENITECRRIQLLAYFGEKGFNPDFCKKHPDVSCDNCCKTKDYKTKDVTDDVKNIVRFVQEHSSSPGTRNIGPAGRFTLNMLVDIFLGSKSAKVQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHLKVDFMETENSSSVKKQKAAVAHVSQRDEAVMKCLGELADVCKLLGKVFGVHYFNIFNTATLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEIIPVLQKYAEWTLPAEGGSPGSRGSRRSTPEETEEEEAVSSHYFANQTRNERKRKKVSASQKPKRRRTSYGGGFRAKGGSTTCRKPSSKSKFSGVTGSRSASCASQATSAASRKLGIMAPPKPVNRPFLRPAYAFS

Target ORF information:

RefSeq Version	XM_003748888.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Bloom syndrome RecQ like helicase (Blm), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003748888.4

ORF Insert Sequence:

ATGTTTGGAT CTCCCGTTGG TTGGATCATG GCTGCTGTTC CTCTGAACAA TCTACAAGAA 
CAACTACAGC GTCACTCGGC CAGAAAACTT AAAAATCAGT TCAGCATTTC CAAACCAAAA 
CCTTCAGGTT TTACTTTTAA AAAGAAAACA CCAGAGGGTG ATGTGTCTGT GGTAAGAACA 
CCTGTGTTAA GCGATAAAGA TGTGAATATG TCTGAGGCCT TGTCATATAC TGAGCCTCCA 
CTCCACAAAC CAAAGCAGCA GTCAAACATC GATGGCTTCT TTAAAAATTC CCCTGGAGGG 
CAGCAAAGCA AGGAGACCTG CTCCGAACTG TCCCTGCCGG AGGTGGTACA GATTCCTCAG 
GACGCTTCTT GTACTACCCC AAAAACCCCA GCTGTGAAGA AACCCCACGT GGCCATTTTC 
AAAAAACTGG AATTTAGTTC TTCGGCAGAC TCTCTCAGTG ATTGGGCTGA TATAGATGAC 
TTTGATGTGC CAGCATCAGA CGCATTTGTT TCCCTGACCA GAAACCCTAC TACAAGAGTG 
AGCACTGCTA AGAAACTGAG TAGCAGTAAG AGAAACTTCT TTAAACCACC ACCTAGAGCA 
AGTGCAGTAA AGGCCGACTT GACTCCTTCC TCCCCCGAAT GCCTGCAAGT GGATTTAACG 
AAGGAACAGG AGGAAGAGGC GGACTGCCTG AGCAGGGATG TGATCTGCAT TGACACTGAC 
CCTGCTTCTG AGGAGCTCTT GGAGAAGGAT GTGCAGGAAG GCCTGTCATT GGACGCTCGC 
TTGGGGACTG AAAGAGTTGA CAGTGCAAAG AAGAGCCATG AAGACAAGGC TGCATTCCAG 
TCACCTCAGA ACGCTCTGTA CTTTGAACAC AATGACAATG ATTATGATAT AGATTTTGTT 
CCACCTTCTC CAGAAGAAAT CATTTCCACT GCTTCTTCCT CGTTGAAATG TTCCAGTATG 
CTAAAGGATC TCGATGACTC TGACAAAGAG AAGGACATTC TGAGTACCTC AAAAGATCTG 
TCAAAACCAG AGGAAATGAC CACACCGAAG TCTGATCTGG GAACCAATGA AGACTGTGAT 
GCCCAGCAGA CTAGCTTGCA GCAGCAGCTT ATCCGTGTGA TGGAGCACAT CTGTAAATTA 
GTTGATACTG TTCCTATTGA TGAACTGAAA GCTCTGACTT GTGGGGACAA ACTGCTTCAG 
CAGCGAGACA TAAGAAGGAA GCTCCTTGCT GAAGCAGGTT TTAATAGAAA CGATGTCAAT 
CTTCTGGGTT CTTTGTGGAG GCACAGGCCA GATTCGCTTG ATAACACTGT GCAGTGTGAC 
TCCTGTCCTG TGGGGCATCC TGGTAAAGAG TTAAACTCTC CATACCTTCC CTCACACTCC 
CTCTCCACTG AGGAATGCTT ACCCACCACT GCTTCAGGAA AGACAGGATT CTCAGCCACC 
CCGAAGAGTC TCTCTGAAAG GCCACCATTG AATTCCCACT TACAGAAGTC CTTTGTAAGT 
AGCAACTGGG CTGAAACACC AAGGACAGAA AGCAGGACGG AAAGCACTTA CTTCCCAGGG 
AGTGTTCTCA CAAGCACCAC TGTGAAGGAT GAGAGTAAAC ATGTTGCTTC AGGACGTGAC 
ATAGAGAGAG AAGTCCAGGC TTCCTACGAT ATTGATAATT TTAACATTGA TGACTTTGAT 
GATGATGATG ATGATGACTG GGAAAACATA ATGCACAATT TTTCAGTCAG CAAATCTTCC 
ACAGCTGCCT ACCCACCTAT CAAGGAAGGT GGCCCAGTTA AATCTCTATC AGAAAGGATT 
TCTTCAGCCA AGGCAAAGTT TCTTCCAGTG GCATCAACCG CTCAAAATAA AAACCTCTCA 
GAGTCAATTC AGAATTGCTC TGATAAGTTT GCACAAAATC TATCATCAAA AAATCCAAAA 
CATGAACATT TTCAAAGTCT TAATTTTCCT CATACAAAAG AAATGATGAA GATTTTCCGT 
AAGAAATTTG GCTTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCGCTG 
CTTGGTGAAG ACTGCTTTAT CCTGATGCCC ACTGGAGGTG GTAAAAGTTT GTGCTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGAGTCACT ATTGTCATTT CTCCCTTGAG ATCACTAATA 
GTGGACCAAG TCCAAAAGCT GACTTCCTTT GATATTCCAG CTACGTACCT GACAGGGGAT 
AAGACTGACT CCGAAGCTGC AAACATTTAC CTCCAGTTGT CAAAGAAAGA CCCGATCATC 
AAGCTTCTGT ATGTCACCCC AGAGAAGGTC TGTGCAAGTA ACAGGCTGAT TTCCACTCTG 
GAGAATCTGT ATGAGCGAAA GCTCTTGGCA CGATTTGTCA TTGATGAAGC ACACTGTGTG 
AGTCAGTGGG GCCATGATTT CCGTCAAGAT TACAAAAGGA TGAATATGCT TCGCCAGAGG 
TTCCCTTCTG TCCCAGTGAT GGCTCTCACG GCCACAGCGA ACCCCAGGGT ACAGAAGGAC 
ATCCTCACGC AGCTGAAGAT TCTCAGGCCT CAGGTGTTTA GCATGAGCTT TAACAGACAC 
AATCTGAAAT ACTATGTATT ACCAAAGAAG CCCAAGAAAG TGGCACTTGA TTGCCTAGAG 
TGGATCAGAA AGCATCACCC TTATGACTCG GGGATAATTT ATTGCCTCTC CAGGAAGGAA 
TGTGACACAA TGGCCGACAC CTTACAGAGA GAAGGTCTTG CTGCTCTGGC TTACCATGCA 
GGTCTTAGTG ATTCTGCCAG AGATGAGGTG CAGCACAAGT GGATCAACCA GGACAACTGC 
CAGGTTATCT GTGCAACCAT TGCATTTGGA ATGGGAATCG ACAAACCTGA CGTGCGATTT 
GTGGTTCATG CATCTCTTCC TAAATCTGTG GAGGGTTATT ACCAAGAATC CGGCCGAGCT 
GGGAGAGACG GGGAAATATC TCACTGCGTG CTTTTCTATA CCTATCATGA CGTGACCAGA 
CTGAAGAGAC TTATAATGAT GGAAAAAGAT GGAAACTATC ATACAAGGGA AACTCACGTC 
AATAATTTAT ATAGCATGGT ACATTACTGC GAAAACATAA CAGAATGCAG AAGAATACAG 
CTTTTAGCTT ACTTTGGTGA AAAAGGATTT AACCCTGATT TTTGTAAGAA ACATCCAGAT 
GTTTCTTGTG ACAATTGCTG TAAAACAAAG GATTATAAAA CAAAAGATGT GACTGATGAT 
GTGAAAAATA TTGTAAGATT TGTTCAAGAA CACAGTTCAT CACCAGGAAC AAGAAATATA 
GGACCTGCTG GAAGATTTAC TCTGAACATG CTAGTCGACA TTTTCTTGGG AAGTAAGAGT 
GCAAAAGTTC AGTCTGGAAT ATTTGGAAAG GGGTCTGCAT ATTCACGCCA CAATGCTGAA 
AGACTTTTTA AAAAGCTGAT TCTAGACAAA ATCTTGGATG AAGACTTATA TATCAATGCC 
AATGACCAAC CAATTGCCTA TGTGATGCTC GGAACAAAAG CCCACAGTGT GCTGAGCGGC 
CACTTGAAGG TGGACTTCAT GGAAACGGAA AATTCGAGCA GTGTTAAAAA ACAAAAAGCC 
GCAGTGGCCC ACGTGTCCCA GAGAGACGAG GCAGTTATGA AGTGTCTGGG AGAACTTGCA 
GACGTCTGCA AATTGCTGGG CAAAGTCTTT GGTGTCCATT ACTTCAATAT TTTCAATACA 
GCCACCCTCA AAAAGCTTGC AGAATCTTTA TCTTCTGATC CTGAGGTTTT GCTTCAGATT 
GATGGTGTTA CTGAAGACAA GCTGGAGAAA TATGGTGCAG AAATAATTCC AGTATTACAG 
AAGTACGCAG AGTGGACATT GCCAGCTGAA GGTGGGTCCC CAGGCAGCAG AGGCTCTAGG 
AGGAGCACCC CAGAGGAGAC GGAGGAGGAG GAAGCTGTAT CTTCTCACTA CTTTGCAAAT 
CAAACTAGAA ATGAAAGAAA GAGGAAGAAA GTGTCAGCCT CCCAAAAGCC CAAGAGAAGA 
AGAACTAGTT ACGGCGGCGG CTTCAGAGCA AAGGGGGGCT CTACTACATG CAGAAAACCA 
TCTTCTAAAA GTAAATTCTC TGGTGTAACT GGATCCCGCT CAGCCTCGTG TGCTTCTCAG 
GCAACATCAG CAGCCAGTAG AAAACTGGGG ATTATGGCTC CTCCAAAGCC TGTAAATAGA 
CCGTTCCTCA GGCCTGCGTA TGCCTTCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Blm cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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