BLM cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	BLM
Entrez Gene ID	415577
Full Name	Bloom syndrome RecQ like helicase
Synonyms	ORF1
General protein information	Preferred Names Bloom syndrome RecQ like helicase Names Bloom syndrome protein homolog BLM helicase Bloom syndrome, RecQ helicase-like Gd BLM recQ helicase homolog
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007087.1	NP_001007088.2	Bloom syndrome protein homolog

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BLM	XP_510594.2
Canis lupus familiaris (dog)	BLM	XP_003434427.1
Mus musculus (house mouse)	Blm	NP_031576.4
	BLM	XP_001097543.1
Gallus gallus (chicken)	BLM	NP_001007088.2
Xenopus tropicalis (tropical clawed frog)	blm	NP_001243837.1
Homo sapiens (human)	BLM	NP_000048.1
Bos taurus (cattle)	BLM	XP_002696629.1
Rattus norvegicus (Norway rat)	Blm	XP_006229520.1
Danio rerio (zebrafish)	blm	XP_701357.4

Gene Ontology
Bibliography

Related articles in PubMed

Purification and enzymatic characterization of Gallus gallus BLM helicase.
Shi J, Liu NN, Yang YT, Xi XG
Journal of biochemistry162(3)183-191(2017 Sep)

A helical bundle in the N-terminal domain of the BLM helicase mediates dimer and potentially hexamer formation.
Shi J, Chen WF, Zhang B, Fan SH, Ai X, Liu NN, Rety S, Xi XG
The Journal of biological chemistry292(14)5909-5920(2017 04)

Bloom DNA helicase facilitates homologous recombination between diverged homologous sequences.
Kikuchi K, Abdel-Aziz HI, Taniguchi Y, Yamazoe M, Takeda S, Hirota K
The Journal of biological chemistry284(39)26360-7(2009 Sep)

Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIalpha.
Otsuki M, Seki M, Inoue E, Abe T, Narita Y, Yoshimura A, Tada S, Ishii Y, Enomoto T
Biochimica et biophysica acta1782(2)75-81(2008 Feb)

Cooperative roles of vertebrate Fbh1 and Blm DNA helicases in avoidance of crossovers during recombination initiated by replication fork collapse.
Kohzaki M, Hatanaka A, Sonoda E, Yamazoe M, Kikuchi K, Vu Trung N, Sz?ts D, Sale JE, Shinagawa H, Watanabe M, Takeda S
Molecular and cellular biology27(8)2812-20(2007 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

first enzymatic characterization of chicken BLM and its N-terminal domain
Title: Purification and enzymatic characterization of Gallus gallus BLM helicase.

BLM is assembled from dimer into hexamer, and also, the stable DHBN dimer can be dissociated upon ATP hydrolysis. Thus, the structural and biochemical characterizations of N-terminal domains will provide new insights into how the N-terminal domain affects the structural and functional organization of the full BLM molecule.
Title: A helical bundle in the N-terminal domain of the BLM helicase mediates dimer and potentially hexamer formation.

Results show that Blm promotes HR between diverged sequences through a novel ATPase-independent mechanism.
Title: Bloom DNA helicase facilitates homologous recombination between diverged homologous sequences.

Recql5/blm double mutant cells showed higher frequency of SCE than blm cells.
Title: Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIalpha.

These data suggest that Fbh1 acts in parallel with Bloom helicase to control recombination-mediated double-strand-break repair at replication blocks and to reduce the frequency of crossover.
Title: Cooperative roles of vertebrate Fbh1 and Blm DNA helicases in avoidance of crossovers during recombination initiated by replication fork collapse.

The following BLM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BLM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa28183	NM_001007087.1 Latest version!	Gallus gallus Bloom syndrome RecQ like helicase (BLM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BLM ( NM_001007087.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001007088.2 Gallus gallus Bloom syndrome RecQ like helicase (BLM), mRNA.

Price & Availability↑

CloneID	OGa28183
Clone ID Related Accession (Same CDS sequence)	NM_001007087.1
Accession Version	NM_001007087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-10
Organism	Gallus gallus(chicken)
Product	Bloom syndrome protein homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ720413.1. On Jan 29, 2005 this sequence version replaced XM_413945.1. ##Evidence-Data-START## Transcript exon combination :: AJ720413.1, AB032092.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG TACCGCAGAA CAACCTCCGG GAGCAGCTGC GGCTGCATTC GGCCCGCGGC 
GCCCTCAGCA AAGTGCTGCC TGCGCGCAGC CGGCCTGCGG CCTTCACCTT CCGCAGGACG 
CACAGCCCGG CCGCCGGGCC CGCGGCGCTG CGCGATAAGG ACGTGAACAC TTCGCTGGGC 
TCGGGAGGCG TCGCCAGGCC CAAGGATCTT CGTGTGGGGC CGCCCGGGGC CCGTCCGGCG 
CTGCCCGGGC CCGCGGGGAG CTCTGGGCCC ACCGCCGGGG CACCGCCCGG CTCAGTGTCC 
GTGTCCTTGC CCTCGTCGGG CCCCGCTGCC TACACGGGCG ATGAGTGGGA CGACATGGAT 
GACTTCGACC TGTCGGGCTT CGAGAAGAAG TTCTCCAGGC CGGCCGTGCT GTCCCCGAAG 
GGCCCGAGGA CTCCTCAGGG GCGCGGTCTG CGTGTCAGTA AGGCTCGGGC TGATGTGGGG 
CCCCAGGAGC ACGGGTCCGA GGTGAGCTGC TCGCAGGGCT CCTCACAGGG CTCCCTCATC 
TGTCTGGGCG ATGCTGGAGC CCCCGCTGCT GCTGTGCTTT CAGACAATGG GATGGAAGAG 
GCTCGCGCAG CTACGAACGG TGGAAGTGGT GAAAGCCAGA AGCTGAGTAA TGGTGAGAAA 
AGCAGCCAGC TGGAACCGGG TGATGTTGGC AATGAGCTGC TGGCAGACAT TGAGCTCGAG 
GAGGATGATT ACCTGGATGT AGTTCCACCC TCTCCAGAGG AAGAGCTGCC TTCCTTCTCG 
CCGTCTGTAA GAAATGTTAG TAATATTTTC AAAGAATCTC CTACTGATGG AAGATCAGCA 
GTTCATGGCA CGGAATCTGA GCCTGAGCTG ATGGCACCAA AGCAGCCTGC TGCTGAGCAG 
GACTCGAGTG CAGAGCATGC AGATAAAGGC TTGCATTTGG AGCAGCAACT CTACAGTGTC 
ATGGAAGATA TTTGTAAGCT GGTGGATGCC ATTCCACTTC ATGAGCTCAC GTCTATCTCA 
TGTGCAAAAG AGCTACTTCA ACAGAGAGAG CTCCGGAGAA AATTGTTAGC TGATTCTGGT 
GCTTTGAACA CAAATAGTGT GAATGGTCCT AGAAATTGGA AGGCATGTGT GCAGCAAGAC 
CCTTCCTCAC GTCCTGGCAC ACCCCTGTGT TCCGGCCCTG GTCGGGGTGT CAGTTCTGTT 
GGCAGCACGC CAAAATCCAC AAACCTTCCA TCTGTGCTTT CCAGGACTGT TAATTCAAGC 
AGTTTTTCTA CTATAAGAAA TCAAACGCTT GATAAGTTGG ACACTTCTTA TTCTTCGAAG 
GAAACTGACC AGGAGGTCAT CTGCCTAGAA CCTGCAGCAC TTCCTTCTCC CAAGGTGAAT 
GGCAAAGGCA GCACCTCTCT CAGCCGTCCC TCGGAGGCAT CATTCAATGG CAGCTGGTGT 
GAAAAACCCA CGGGGAGAGA CAGTGGGAAC TGGCGTGTGC CCGAGAGGCC CACTGCAAGC 
ACTGCTCTGA AAGCCCAGCA CACAGCCCCT GCTGGCAATC CTGCATCGGG CTGCTGGGAT 
GTGAACGATA CAGACTTCGA TCTGGACCAC TTTGATATTG ATGATTTTGA TGAGGGCTGG 
GAGGAGGCTG TGGCACCTGA AGCAGCACCT GAAGCACCAC CTGCGCCCCA GTGGCAGCCG 
CTGAGGGAGG GCTCTGCCTC TCTGCGCTGC AGGCTGCTCG CTGCGGCCGC TGGCTCTGCT 
CCGGGCCCAC ATCCCACTGC TCCAAAGTCA GGCTGTGGGA TCTCAGCGAA GAGCAGCTCA 
GAGCCACTGG TCCATAACCC TGCACACGAA CGTTTCAGGG GGATGAAATT TTCCCATTCT 
GAAGAAATGT TGAAAATATT TCACAGGAAG TTTGGTTTGC ACTCTTTCCG GACAAATCAG 
CTGGAGGCCA TCAATGCTGC TCTTCTGGGA GAAGACTGTT TCATCCTAAT GCCCACTGGT 
GGTGGTAAAA GTCTGTGCTA CCAGTTACCA GCGTGTGTCT CTGCTGGGGT CACTGTTGTC 
ATTTCTCCCC TGAGGTCACT GATAATAGAC CAAGTTCAGA AGCTGAAAAC TTTAGACATT 
GCTTCAACGT ATCTGACAGG TGATATAACA GATGCTGATG CTTCAAAAAC ATATATGCAG 
CTGTCAAAAA AAGATCCTAT AATAAAGCTT CTGTATGTTA CCCCTGAGAA GGTTTGTGCA 
AGCAACCGAC TGCTGTCAGC CCTGGAAAAT CTCTACAACC GGAAACTGCT CGCGCGTTTT 
GTCATTGACG AGGCCCACTG TGTTAGCCAG TGGGGCCATG ACTTCCGAAA AGACTACAAA 
CGGCTGAATA TGCTCCGCAA GAAGTTCCAC TCTGTTCCTA TGATGGCTCT CACTGCCACT 
GCTAACCCCA GAGTGCAGAA GGATATTCAG AATCAGCTGG AGATGCTAAA ACCACAAGTG 
TTTACAATGA GCTTCAACAG GCATAACTTG AAATATGATG TACTGCCCAA GAAGCCAAAG 
AAGGTGGCAA TGGATTGCTT GGAGTGGATT AAAAAATACC ATCCACATGA CTCTGGGATA 
ATTTATTGCC TTTCCCGTCA TGAATGTGAC ACGACAGCAG CCATTCTGCA GAAGGAAGGC 
CTTGCTGCAC TTGCCTATCA TGCTGGCCTC ACTGACTCCA ACAGAGATCT TGTACAGAAG 
AAGTGGGTTA ATCAAGAAGG GTGCCAGGTT ATATGTGCAA CGATTGCCTT TGGAATGGGA 
ATAGATAAAC CTGACGTACG CTATGTTATC CATGCTTCCC TTCCAAAATC TATAGAAGGC 
TACTATCAGG AGTCGGGCAG GGCTGGGCGG GATGGTGAAA TGTCTCACTG CCTGCTCTTC 
TACAGCTACA GCGACGTCAC CAGGCTCAGG AGGCTCATAC TGATGGAGAA GGATGGAAAC 
AGTCACACGA GACAGACCCA CTTCAACAAC CTGTACAGCA TGGTGCATTA CTGCGAGAAC 
GTCGTTGACT GCCGGAGAAT TCAGCTTCTG GCCTACTTTG GGGAGACTGA TTTTAATCCT 
AACTTTTGTA AAGATCACCC AGAAGTTATT TGTGATAACT GCAGTAGAAA GAAGGATTAC 
AAATCAAGAA ATGTAACAGA TGAAGTGAAA AGCATCATAA GATTTGTACA GCAACACTGT 
GGGCAAGTGG GAGGAATAAA TGGAAATAGA AACACAGGCT CTGGAAGATA CACACTGAAT 
ATGATGGTAG ACATTTTCTT GGGAGCAAAG AGCGCGAAGA TTCAGTCCGG AATATTTGGG 
AAGGGCGCTG CCTACTCACG GCACAACGTT GAAAGGCTGT TCAGAAAGCT GGTCTTGGAC 
AAGATCCTAG ATGAAGACCT GTACATCACA GCTAATGACC AAGCTGTGGC CTACGTAGTT 
CTAGGAGAAA AAGCGCAGGC GGTGCTAAAT GGGTTGCTGC AGGTGGAATT TCATGAAACT 
GAAAATGCCA GTGCTATTAG AAAGCAAAGG GCTTCTGTGA CAAAAATGTC CCAGCGGGAA 
GAGATGGTCA AAAAGTGCCT TGGGGAACTT ACAGACACCT GCAAAACTCT CGGGAAAATC 
TTTGATGTCC ATTACTTCAA TATTTTCAGT ACTTCAACTC TAAAGAAAAT AGCAGAAACC 
TTGTCTTCTG ATGCGGAGGT TTTGCTGCAG ATTGATGGTG TCACAGAAGA TAAACTGGAG 
AAATATGGTG CAGAAATCAT TAAAGTGATG GATAAGTACT CTGAATGGAC AACACCAGAA 
GATGCTGCCT GCCAGAGTGT GGACACGGCC CCGGGGAGCG CTGGCACGCC TGGGAGTGAG 
GAGGAAGCAG CAGACGACGT TGTGACCTCG AGCTATTTTG GTGGCAATGC AAACCAAAGA 
AGGAAGAGGA AAAGGCTGCC AAACTCCGGA GAATCCAAGA GGAAAAAGAC AAGCAGTGGT 
GGCAGCCAGC AGTTTTATTC CAAAGGGGCC AGGTACCGGC GGGCACGAAG GGCGCCCGGC 
TCCAGGGCTG CAGCTCCTGC ACAAAGCTCG GCACTGCGTG GCGCCGGGGC ACGGCTGGGC 
ATCATGGCAC CACCCAAACC CAGCAGCAGG CACTTCCTGC AGCCGTCCTA CGCCGTGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007088.2
CDS	115..4257
Translation	MAAVPQNNLREQLRLHSARGALSKVLPARSRPAAFTFRRTHSPAAGPAALRDKDVNTSLGSGGVARPKDLRVGPPGARPALPGPAGSSGPTAGAPPGSVSVSLPSSGPAAYTGDEWDDMDDFDLSGFEKKFSRPAVLSPKGPRTPQGRGLRVSKARADVGPQEHGSEVSCSQGSSQGSLICLGDAGAPAAAVLSDNGMEEARAATNGGSGESQKLSNGEKSSQLEPGDVGNELLADIELEEDDYLDVVPPSPEEELPSFSPSVRNVSNIFKESPTDGRSAVHGTESEPELMAPKQPAAEQDSSAEHADKGLHLEQQLYSVMEDICKLVDAIPLHELTSISCAKELLQQRELRRKLLADSGALNTNSVNGPRNWKACVQQDPSSRPGTPLCSGPGRGVSSVGSTPKSTNLPSVLSRTVNSSSFSTIRNQTLDKLDTSYSSKETDQEVICLEPAALPSPKVNGKGSTSLSRPSEASFNGSWCEKPTGRDSGNWRVPERPTASTALKAQHTAPAGNPASGCWDVNDTDFDLDHFDIDDFDEGWEEAVAPEAAPEAPPAPQWQPLREGSASLRCRLLAAAAGSAPGPHPTAPKSGCGISAKSSSEPLVHNPAHERFRGMKFSHSEEMLKIFHRKFGLHSFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSAGVTVVISPLRSLIIDQVQKLKTLDIASTYLTGDITDADASKTYMQLSKKDPIIKLLYVTPEKVCASNRLLSALENLYNRKLLARFVIDEAHCVSQWGHDFRKDYKRLNMLRKKFHSVPMMALTATANPRVQKDIQNQLEMLKPQVFTMSFNRHNLKYDVLPKKPKKVAMDCLEWIKKYHPHDSGIIYCLSRHECDTTAAILQKEGLAALAYHAGLTDSNRDLVQKKWVNQEGCQVICATIAFGMGIDKPDVRYVIHASLPKSIEGYYQESGRAGRDGEMSHCLLFYSYSDVTRLRRLILMEKDGNSHTRQTHFNNLYSMVHYCENVVDCRRIQLLAYFGETDFNPNFCKDHPEVICDNCSRKKDYKSRNVTDEVKSIIRFVQQHCGQVGGINGNRNTGSGRYTLNMMVDIFLGAKSAKIQSGIFGKGAAYSRHNVERLFRKLVLDKILDEDLYITANDQAVAYVVLGEKAQAVLNGLLQVEFHETENASAIRKQRASVTKMSQREEMVKKCLGELTDTCKTLGKIFDVHYFNIFSTSTLKKIAETLSSDAEVLLQIDGVTEDKLEKYGAEIIKVMDKYSEWTTPEDAACQSVDTAPGSAGTPGSEEEAADDVVTSSYFGGNANQRRKRKRLPNSGESKRKKTSSGGSQQFYSKGARYRRARRAPGSRAAAPAQSSALRGAGARLGIMAPPKPSSRHFLQPSYAVL

Target ORF information:

RefSeq Version	NM_001007087.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Bloom syndrome RecQ like helicase (BLM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007087.1

ORF Insert Sequence:

ATGGCGGCGG TACCGCAGAA CAACCTCCGG GAGCAGCTGC GGCTGCATTC GGCCCGCGGC 
GCCCTCAGCA AAGTGCTGCC TGCGCGCAGC CGGCCTGCGG CCTTCACCTT CCGCAGGACG 
CACAGCCCGG CCGCCGGGCC CGCGGCGCTG CGCGATAAGG ACGTGAACAC TTCGCTGGGC 
TCGGGAGGCG TCGCCAGGCC CAAGGATCTT CGTGTGGGGC CGCCCGGGGC CCGTCCGGCG 
CTGCCCGGGC CCGCGGGGAG CTCTGGGCCC ACCGCCGGGG CACCGCCCGG CTCAGTGTCC 
GTGTCCTTGC CCTCGTCGGG CCCCGCTGCC TACACGGGCG ATGAGTGGGA CGACATGGAT 
GACTTCGACC TGTCGGGCTT CGAGAAGAAG TTCTCCAGGC CGGCCGTGCT GTCCCCGAAG 
GGCCCGAGGA CTCCTCAGGG GCGCGGTCTG CGTGTCAGTA AGGCTCGGGC TGATGTGGGG 
CCCCAGGAGC ACGGGTCCGA GGTGAGCTGC TCGCAGGGCT CCTCACAGGG CTCCCTCATC 
TGTCTGGGCG ATGCTGGAGC CCCCGCTGCT GCTGTGCTTT CAGACAATGG GATGGAAGAG 
GCTCGCGCAG CTACGAACGG TGGAAGTGGT GAAAGCCAGA AGCTGAGTAA TGGTGAGAAA 
AGCAGCCAGC TGGAACCGGG TGATGTTGGC AATGAGCTGC TGGCAGACAT TGAGCTCGAG 
GAGGATGATT ACCTGGATGT AGTTCCACCC TCTCCAGAGG AAGAGCTGCC TTCCTTCTCG 
CCGTCTGTAA GAAATGTTAG TAATATTTTC AAAGAATCTC CTACTGATGG AAGATCAGCA 
GTTCATGGCA CGGAATCTGA GCCTGAGCTG ATGGCACCAA AGCAGCCTGC TGCTGAGCAG 
GACTCGAGTG CAGAGCATGC AGATAAAGGC TTGCATTTGG AGCAGCAACT CTACAGTGTC 
ATGGAAGATA TTTGTAAGCT GGTGGATGCC ATTCCACTTC ATGAGCTCAC GTCTATCTCA 
TGTGCAAAAG AGCTACTTCA ACAGAGAGAG CTCCGGAGAA AATTGTTAGC TGATTCTGGT 
GCTTTGAACA CAAATAGTGT GAATGGTCCT AGAAATTGGA AGGCATGTGT GCAGCAAGAC 
CCTTCCTCAC GTCCTGGCAC ACCCCTGTGT TCCGGCCCTG GTCGGGGTGT CAGTTCTGTT 
GGCAGCACGC CAAAATCCAC AAACCTTCCA TCTGTGCTTT CCAGGACTGT TAATTCAAGC 
AGTTTTTCTA CTATAAGAAA TCAAACGCTT GATAAGTTGG ACACTTCTTA TTCTTCGAAG 
GAAACTGACC AGGAGGTCAT CTGCCTAGAA CCTGCAGCAC TTCCTTCTCC CAAGGTGAAT 
GGCAAAGGCA GCACCTCTCT CAGCCGTCCC TCGGAGGCAT CATTCAATGG CAGCTGGTGT 
GAAAAACCCA CGGGGAGAGA CAGTGGGAAC TGGCGTGTGC CCGAGAGGCC CACTGCAAGC 
ACTGCTCTGA AAGCCCAGCA CACAGCCCCT GCTGGCAATC CTGCATCGGG CTGCTGGGAT 
GTGAACGATA CAGACTTCGA TCTGGACCAC TTTGATATTG ATGATTTTGA TGAGGGCTGG 
GAGGAGGCTG TGGCACCTGA AGCAGCACCT GAAGCACCAC CTGCGCCCCA GTGGCAGCCG 
CTGAGGGAGG GCTCTGCCTC TCTGCGCTGC AGGCTGCTCG CTGCGGCCGC TGGCTCTGCT 
CCGGGCCCAC ATCCCACTGC TCCAAAGTCA GGCTGTGGGA TCTCAGCGAA GAGCAGCTCA 
GAGCCACTGG TCCATAACCC TGCACACGAA CGTTTCAGGG GGATGAAATT TTCCCATTCT 
GAAGAAATGT TGAAAATATT TCACAGGAAG TTTGGTTTGC ACTCTTTCCG GACAAATCAG 
CTGGAGGCCA TCAATGCTGC TCTTCTGGGA GAAGACTGTT TCATCCTAAT GCCCACTGGT 
GGTGGTAAAA GTCTGTGCTA CCAGTTACCA GCGTGTGTCT CTGCTGGGGT CACTGTTGTC 
ATTTCTCCCC TGAGGTCACT GATAATAGAC CAAGTTCAGA AGCTGAAAAC TTTAGACATT 
GCTTCAACGT ATCTGACAGG TGATATAACA GATGCTGATG CTTCAAAAAC ATATATGCAG 
CTGTCAAAAA AAGATCCTAT AATAAAGCTT CTGTATGTTA CCCCTGAGAA GGTTTGTGCA 
AGCAACCGAC TGCTGTCAGC CCTGGAAAAT CTCTACAACC GGAAACTGCT CGCGCGTTTT 
GTCATTGACG AGGCCCACTG TGTTAGCCAG TGGGGCCATG ACTTCCGAAA AGACTACAAA 
CGGCTGAATA TGCTCCGCAA GAAGTTCCAC TCTGTTCCTA TGATGGCTCT CACTGCCACT 
GCTAACCCCA GAGTGCAGAA GGATATTCAG AATCAGCTGG AGATGCTAAA ACCACAAGTG 
TTTACAATGA GCTTCAACAG GCATAACTTG AAATATGATG TACTGCCCAA GAAGCCAAAG 
AAGGTGGCAA TGGATTGCTT GGAGTGGATT AAAAAATACC ATCCACATGA CTCTGGGATA 
ATTTATTGCC TTTCCCGTCA TGAATGTGAC ACGACAGCAG CCATTCTGCA GAAGGAAGGC 
CTTGCTGCAC TTGCCTATCA TGCTGGCCTC ACTGACTCCA ACAGAGATCT TGTACAGAAG 
AAGTGGGTTA ATCAAGAAGG GTGCCAGGTT ATATGTGCAA CGATTGCCTT TGGAATGGGA 
ATAGATAAAC CTGACGTACG CTATGTTATC CATGCTTCCC TTCCAAAATC TATAGAAGGC 
TACTATCAGG AGTCGGGCAG GGCTGGGCGG GATGGTGAAA TGTCTCACTG CCTGCTCTTC 
TACAGCTACA GCGACGTCAC CAGGCTCAGG AGGCTCATAC TGATGGAGAA GGATGGAAAC 
AGTCACACGA GACAGACCCA CTTCAACAAC CTGTACAGCA TGGTGCATTA CTGCGAGAAC 
GTCGTTGACT GCCGGAGAAT TCAGCTTCTG GCCTACTTTG GGGAGACTGA TTTTAATCCT 
AACTTTTGTA AAGATCACCC AGAAGTTATT TGTGATAACT GCAGTAGAAA GAAGGATTAC 
AAATCAAGAA ATGTAACAGA TGAAGTGAAA AGCATCATAA GATTTGTACA GCAACACTGT 
GGGCAAGTGG GAGGAATAAA TGGAAATAGA AACACAGGCT CTGGAAGATA CACACTGAAT 
ATGATGGTAG ACATTTTCTT GGGAGCAAAG AGCGCGAAGA TTCAGTCCGG AATATTTGGG 
AAGGGCGCTG CCTACTCACG GCACAACGTT GAAAGGCTGT TCAGAAAGCT GGTCTTGGAC 
AAGATCCTAG ATGAAGACCT GTACATCACA GCTAATGACC AAGCTGTGGC CTACGTAGTT 
CTAGGAGAAA AAGCGCAGGC GGTGCTAAAT GGGTTGCTGC AGGTGGAATT TCATGAAACT 
GAAAATGCCA GTGCTATTAG AAAGCAAAGG GCTTCTGTGA CAAAAATGTC CCAGCGGGAA 
GAGATGGTCA AAAAGTGCCT TGGGGAACTT ACAGACACCT GCAAAACTCT CGGGAAAATC 
TTTGATGTCC ATTACTTCAA TATTTTCAGT ACTTCAACTC TAAAGAAAAT AGCAGAAACC 
TTGTCTTCTG ATGCGGAGGT TTTGCTGCAG ATTGATGGTG TCACAGAAGA TAAACTGGAG 
AAATATGGTG CAGAAATCAT TAAAGTGATG GATAAGTACT CTGAATGGAC AACACCAGAA 
GATGCTGCCT GCCAGAGTGT GGACACGGCC CCGGGGAGCG CTGGCACGCC TGGGAGTGAG 
GAGGAAGCAG CAGACGACGT TGTGACCTCG AGCTATTTTG GTGGCAATGC AAACCAAAGA 
AGGAAGAGGA AAAGGCTGCC AAACTCCGGA GAATCCAAGA GGAAAAAGAC AAGCAGTGGT 
GGCAGCCAGC AGTTTTATTC CAAAGGGGCC AGGTACCGGC GGGCACGAAG GGCGCCCGGC 
TCCAGGGCTG CAGCTCCTGC ACAAAGCTCG GCACTGCGTG GCGCCGGGGC ACGGCTGGGC 
ATCATGGCAC CACCCAAACC CAGCAGCAGG CACTTCCTGC AGCCGTCCTA CGCCGTGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Purification and enzymatic characterization of Gallus gallus BLM helicase.
Journal of biochemistry162(3)183-191(2017 Sep)
Shi J,Liu NN,Yang YT,Xi XG

A helical bundle in the N-terminal domain of the BLM helicase mediates dimer and potentially hexamer formation.
The Journal of biological chemistry292(14)5909-5920(2017 04)
Shi J,Chen WF,Zhang B,Fan SH,Ai X,Liu NN,Rety S,Xi XG

Bloom DNA helicase facilitates homologous recombination between diverged homologous sequences.
The Journal of biological chemistry284(39)26360-7(2009 Sep)
Kikuchi K,Abdel-Aziz HI,Taniguchi Y,Yamazoe M,Takeda S,Hirota K

Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIalpha.
Biochimica et biophysica acta1782(2)75-81(2008 Feb)
Otsuki M,Seki M,Inoue E,Abe T,Narita Y,Yoshimura A,Tada S,Ishii Y,Enomoto T

Cooperative roles of vertebrate Fbh1 and Blm DNA helicases in avoidance of crossovers during recombination initiated by replication fork collapse.
Molecular and cellular biology27(8)2812-20(2007 Apr)
Kohzaki M,Hatanaka A,Sonoda E,Yamazoe M,Kikuchi K,Vu Trung N,Sz?ts D,Sale JE,Shinagawa H,Watanabe M,Takeda S

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BLM cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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