BLM cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	BLM
Entrez Gene ID	572540
Full Name	Bloom syndrome, RecQ helicase-like
General protein information	Preferred Names Bloom syndrome, RecQ helicase-like Names Bloom syndrome protein
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_696265.6	XP_701357.5	Bloom syndrome protein isoform X3
XM_021467812.1	XP_021323487.1	Bloom syndrome protein isoform X2
XM_696265.7	XP_701357.5	Bloom syndrome protein isoform X3
XM_017352050.1	XP_017207539.1	Bloom syndrome protein isoform X2
XM_017352049.2	XP_017207538.1	Bloom syndrome protein isoform X1
XM_017352050.2	XP_017207539.1	Bloom syndrome protein isoform X2
XM_017352049.1	XP_017207538.1	Bloom syndrome protein isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BLM	XP_510594.2
Canis lupus familiaris (dog)	BLM	XP_003434427.1
Mus musculus (house mouse)	Blm	NP_031576.4
	BLM	XP_001097543.1
Gallus gallus (chicken)	BLM	NP_001007088.2
Xenopus tropicalis (tropical clawed frog)	blm	NP_001243837.1
Homo sapiens (human)	BLM	NP_000048.1
Bos taurus (cattle)	BLM	XP_002696629.1
Rattus norvegicus (Norway rat)	Blm	XP_006229520.1
Danio rerio (zebrafish)	blm	XP_701357.4

Gene Ontology

The following BLM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BLM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa55902	XM_017352049.2 Latest version!	Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa55903	XM_017352050.2 Latest version!	Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa55903	XM_021467812.1 Latest version!	Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa55904	XM_696265.7 Latest version!	Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
View Old Accession Versions >>
ODa55904	XM_696265.6	Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa55903	XM_017352050.1	Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa55902	XM_017352049.1	Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BLM ( XM_017352049.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207538.1 Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa55902
Clone ID Related Accession (Same CDS sequence)	XM_017352049.2 , XM_017352049.1
Accession Version	XM_017352049.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Bloom syndrome protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAATG AGAGAGCGAG TTGCAAATAC GCGATGTCGA GTCTTCCTCA AAACAACCTG 
AAGGAGCATC TGGAGCGACA CAACAATGCT GCCCAGAACA AGCTGTCACT GCTCAAACCA 
AAGCCTGGGG GGTTTTGCTT CAAAAAGAAG TCTTCGTCAG GCATCTCCAA AGTGGAAGTT 
CCTCAAAAGG TAACTGGTTC AAGTGTCTTA GCAAACAGGA GTGTCAATAT TCCCAGCAAC 
TTTGAAGTAA CCAAAAATCC TGTGACGTTT TCGCAAAAGC CAGAGAGAGT GGCACCGAAA 
GTCAACTTCT TCACTGCGCC CAGCAAACCG AAGACAAATA CAGTTAATCC ACTAGCAAAC 
CCATTCGCTT TGAACAAAAC CCCTCTTGTC CAATCTACCA TTAAGGCTTT GCCCAAATCT 
GCAGCCTTGC CTAAGGACTC AACTAATGAT CAAACTGTGG AGCTTAAGCA TGACATTTCT 
CAACTCTCAT TCAATGAATG GGATGACTTG GATGATTTTG AAACTCCAGT CAAGAGCAGA 
GTAGCATCCC CAGTTGCAGG GACCTCTACG AAAAAGCCAA GTGTATCAGA CCAGAACACA 
TCCTCAAGCT GCTCTTCTAA GTGTGAGGAG ACTAAAGTAA ATGAGGAGTC ACAAGCTACA 
GAAACCATAA CTGCCCCGAA GGACGTCCTG TCAGCTGCTA ATGGAGTCAG CACAGAGACT 
GCAGAGAGAG AGCCAGAGGA CTCACCCATT AAAAAGTCCA AAAGACCTAA GAAATCAGTC 
CAGCATACAG CATTACTTAG TGACACGGAA GATGAGGAAA TAATCCACTG TGTTTCACCG 
GATACAAACC AGAAGAATTT CAAAAGTGTG GCAGTTGCTG AAAAAGATAA ATGGGGTGAG 
CCAAATGTCA TAGACAGTGA TGACTGTGAG GAGAACAACC ATTATGAAGG TTTTGAGGAT 
TTCATCCCCC CGTCCCCTAT TCCAGAAGAG ATCAGTCTCT CTGTCTCAGA TAAGGAGAAA 
AGTTCATCTG AGCCTGTTAC TCCTGCTAAC AAAAAAGAAT CTTCTCGATC TGCCTCAGGC 
CTGCCAGCAC CCTTGGATCA ATCTGCCAAA GGATTAAAAG GAGCTGATGA CGCTCTCTTT 
AGTGTTATGG AATCTATCTG TTGTCTAGTG GACACTATTC CTGAGCATGA GCTCATAGCG 
CTGACCTGTG GGACTGAGCT TTTACTGCAG AGAGCACACA GGAAAAGGAT TCTTGCTAAA 
GGTGGGTCAT CTAGAACATC TCATTCTGAC TCAGTATCAA CTCCTGGTTT CCTAAACAGA 
CCTACGTTTG GAGTTACACC CTCCAATCTG ACATCCCTGA CCCCGGTAAC TTCAGGAAAA 
AGAGAAGGTG TCAAAACTGG CTTTTCTTTT CGCAAATCCA TTGCCTCTGT CATGTCTGTG 
GGTGATGAGA GTGTTTTTGA GGATTCTGAC TGCATCATAA ATGGAGTCGA AACACCTGGT 
GGTACCTGGA ATCCTAACAG CTCCACTAAA ATATCAGCTG GTAGGGATAC CTTTAACGGG 
TCAATCCAAA CTTTAAGCAA ACCTGAGTCA AAGACAGATA AATGCTACTC AAGGTTGTCC 
TTCAATGAGT CAAGCAATCA GACTGACCTG TTTTATTCCC CCAAGAGAGT GGATTCAGGC 
AGCAGAAATG CTGACAGCAG TGTTGAGATC AACATTGCAG GATCCAGCTC TTTACGAACA 
GGAGCAGAGC CAGTTGATGA TTTTTTACTC GATGACTTCG ATATTGATGA TTTTGATGAG 
AATGATATTC CAGATTATTA TGAAGAACCT CCAAGTGTCT TGGAATCAAG AAATAACTCT 
GGTGTAAAAA CACCATCAGT ACAGGAGGGA GGATCATCAA AATCTTTTGA GAGGAAGACA 
TTCACACCAC CAGCACCAAA GTCTATTAAA ACTCCCAACC CAGAACCTTT GTACAGGAAC 
CCAGCTCATG ACCGCTTCAG AGGCTTTAAC TTTCCTCATA GCCCAGAGAT GATGAAGATC 
TTCCATAAGA AGTTTGGACT TCATCAGTTC CGCTTTAACC AGCTGGAAGC TATTAATGCC 
TCGCTGTTAG GAGAAGACAC ATTTGTATTG ATGCCTACAG GTGGTGGTAA AAGTCTCTGT 
TATCAGCTTC CTGCTTGTGT CTCTGCTGGG GTGACTGTAG TCATTTCTCC TCTACGCTCT 
TTAATAGTTG ACCAAGTACA AAAACTCACC ACGTTAGATA TCTGTGCAAC TAGTTTGTCC 
GGGGACAAAA AAGATAGTGA GGCTGCCCGA ATCTACATGC AGCTGTCCAG GAAAGATCCT 
GCCATCAAAC TGCTTTATGC TACTCCTGAG AAGGTTTGTG CAAGTGGGCG GATGATCAGT 
GCACTTCAGA ATCTGTATGA AAGAGGTCTG CTGGCTCGCT TGGTCATTGA TGAGGCCCAT 
TGCGTCAGTC AGTGGGGTCA TGATTTCAGA CCAGACTACA AGCGCTTGCA TGAGCTCAGG 
CGGATGTTTC CCAATGTTCC TATAATGGCA CTGACGGCCA CAGCCACTCC TAGAGTTCAG 
AAAGACATTC TTAACCAATT GGCAATGACA CGTCCTCAGG TATTTACTAT GAGCTTTAAC 
AGGAACAATC TGAAATACTC AGTTTTGCCT AAGAAGCCCA AGAAGGTTGA TGAGGAATGC 
ATTCAGTGGA TTAAGAAATA TTACCCACGT GACTCTGGGA TTGTGTACTG CTTGTCTCGT 
AATGACTGCG ACACTCTGGC TGACAGTCTT CAGAGGGCAG GGATCGCAGC GCTGGCGTAT 
CATGCTGGCC TAAGTGACAG CGACAGGGAA TATGTCCAGA ACAAGTGGAT CAACCAGGAC 
GGCTGTCAGG TTATGTGCGC AACCATAGCA TTTGGCATGG GAATCGATAA GCCTGATGTC 
CGTTATGTGA TCCATGCGAG TCTCCCCAAA TCAGTGGAGG GTTACTACCA GGAGTCAGGC 
AGAGCAGGGC GAGATGGAGA GATTTCCCAT TGTGTCCTCT TCTATTCCTA CAGTGACGTT 
ATCCGCATCA AGAGACTCAT TGCTATGGAT AAAGATGGCA ACCAGCAATC CAAAGCCACC 
CACATCAATA ACCTGCACAG CATGGTGCAC TTCTGTGAGA ATGTGGCTGA ATGCAGAAGG 
ATCCAGCTGC TGGCTTACTT TGGGGAGCAC ACGTTTAACA CGAGCTTCTG TAAAGAGCAT 
CCTGAGGTCA TCTGTGACAA CTGTGCCAGA CCAAACAAAT ATAAATCCAG AAATGTGACC 
GATGACGTTA AGAAGATTGC GAGGTTTGTG CAGGACAACT GTGAGAAGGT TGGAAACAGA 
TACGGCAAAT CCGCCCAGCA GAACAGGCTC ACTCTCAACA TGCTGGTTGA AATATTTCTG 
GGCTCCAAAA GCGCTCGCAT CCAGACTGGG ATGTTCGGAG TTGGAGCAGC TTATTCCAAA 
CACAACGCTG AGAGGCTCTT TAAGAAGCTG GTGCTGGATA ATGTTCTGAT GGAGGATCTG 
TACATCACTA ACAGTGGCCA GGCTGTGGCC TATATCTCTG CAGGCCCTAA AGCTATGAGT 
GTACTGAATG GTTGCATGCA GGTTGAATTT CATGAGACCG AGAGCGCATC CAGCATCAGA 
AAGCACAGAG CGTCTGTGTG TGAGAAGGTG TCTAAGAGAG AGGAGATGGT GAAAAAGTGT 
CTGGGTGAAC TTAATGATCT ATGCAAGAAG TTGGGCAAAG TCTTCGGCAT TCATTACTAC 
AATATCTTCT CCACCGCTAC ACTCAAAAAG ATTGCTGAGA CTCTCTCGGC TGATCCGGAG 
GTTCTGCTGC AGATTGATGG TGTAACTGAA GACAAACTGG AGAAATATGG AGCTGAGTTT 
ATTGAACTGC TACAGAAATA CTCTGAGTGG CAGCTACCTG CTGAAGCACA GGCTGAGAGT 
TCAGGCTGGA TCGACACGAC CAGAGGCCAT CAAAATGATG AAGACGACAA CGATGATGAT 
GATGAAGGAG GTGGGGATGT CACATCGACA TATTTCAGAA GCAACTCTGG ACGAGGAGCC 
AAGAGAAAGC AAGGCTCTTA CTCCAGAAAA CCCAAACGAA GAAAAGGCTC CAGTGGCCAG 
AACTCCTCTG CAAAAGGTGG ATATAGCAGT AACTGGTCTT CATCACGAGG AGGAGGAAGA 
GGAGGTGGAT ACAGGGGTGG CAGTCGAGGA GCAGGCCGAG GCTCCAGATC TGCTCCTTCA 
GGCTCAGCAG CAAAAAGGCC GGGCTTCATG TCGTTACCAA CACCGCAGGC TGCTGCTCGT 
CCATTCCTCA AACCGTCCTT CTCACACCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207538.1
CDS	332..4684
Translation	MTNERASCKYAMSSLPQNNLKEHLERHNNAAQNKLSLLKPKPGGFCFKKKSSSGISKVEVPQKVTGSSVLANRSVNIPSNFEVTKNPVTFSQKPERVAPKVNFFTAPSKPKTNTVNPLANPFALNKTPLVQSTIKALPKSAALPKDSTNDQTVELKHDISQLSFNEWDDLDDFETPVKSRVASPVAGTSTKKPSVSDQNTSSSCSSKCEETKVNEESQATETITAPKDVLSAANGVSTETAEREPEDSPIKKSKRPKKSVQHTALLSDTEDEEIIHCVSPDTNQKNFKSVAVAEKDKWGEPNVIDSDDCEENNHYEGFEDFIPPSPIPEEISLSVSDKEKSSSEPVTPANKKESSRSASGLPAPLDQSAKGLKGADDALFSVMESICCLVDTIPEHELIALTCGTELLLQRAHRKRILAKGGSSRTSHSDSVSTPGFLNRPTFGVTPSNLTSLTPVTSGKREGVKTGFSFRKSIASVMSVGDESVFEDSDCIINGVETPGGTWNPNSSTKISAGRDTFNGSIQTLSKPESKTDKCYSRLSFNESSNQTDLFYSPKRVDSGSRNADSSVEINIAGSSSLRTGAEPVDDFLLDDFDIDDFDENDIPDYYEEPPSVLESRNNSGVKTPSVQEGGSSKSFERKTFTPPAPKSIKTPNPEPLYRNPAHDRFRGFNFPHSPEMMKIFHKKFGLHQFRFNQLEAINASLLGEDTFVLMPTGGGKSLCYQLPACVSAGVTVVISPLRSLIVDQVQKLTTLDICATSLSGDKKDSEAARIYMQLSRKDPAIKLLYATPEKVCASGRMISALQNLYERGLLARLVIDEAHCVSQWGHDFRPDYKRLHELRRMFPNVPIMALTATATPRVQKDILNQLAMTRPQVFTMSFNRNNLKYSVLPKKPKKVDEECIQWIKKYYPRDSGIVYCLSRNDCDTLADSLQRAGIAALAYHAGLSDSDREYVQNKWINQDGCQVMCATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCVLFYSYSDVIRIKRLIAMDKDGNQQSKATHINNLHSMVHFCENVAECRRIQLLAYFGEHTFNTSFCKEHPEVICDNCARPNKYKSRNVTDDVKKIARFVQDNCEKVGNRYGKSAQQNRLTLNMLVEIFLGSKSARIQTGMFGVGAAYSKHNAERLFKKLVLDNVLMEDLYITNSGQAVAYISAGPKAMSVLNGCMQVEFHETESASSIRKHRASVCEKVSKREEMVKKCLGELNDLCKKLGKVFGIHYYNIFSTATLKKIAETLSADPEVLLQIDGVTEDKLEKYGAEFIELLQKYSEWQLPAEAQAESSGWIDTTRGHQNDEDDNDDDDEGGGDVTSTYFRSNSGRGAKRKQGSYSRKPKRRKGSSGQNSSAKGGYSSNWSSSRGGGRGGGYRGGSRGAGRGSRSAPSGSAAKRPGFMSLPTPQAAARPFLKPSFSHL

Target ORF information:

RefSeq Version	XM_017352049.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352049.2

ORF Insert Sequence:

ATGACCAATG AGAGAGCGAG TTGCAAATAC GCGATGTCGA GTCTTCCTCA AAACAACCTG 
AAGGAGCATC TGGAGCGACA CAACAATGCT GCCCAGAACA AGCTGTCACT GCTCAAACCA 
AAGCCTGGGG GGTTTTGCTT CAAAAAGAAG TCTTCGTCAG GCATCTCCAA AGTGGAAGTT 
CCTCAAAAGG TAACTGGTTC AAGTGTCTTA GCAAACAGGA GTGTCAATAT TCCCAGCAAC 
TTTGAAGTAA CCAAAAATCC TGTGACGTTT TCGCAAAAGC CAGAGAGAGT GGCACCGAAA 
GTCAACTTCT TCACTGCGCC CAGCAAACCG AAGACAAATA CAGTTAATCC ACTAGCAAAC 
CCATTCGCTT TGAACAAAAC CCCTCTTGTC CAATCTACCA TTAAGGCTTT GCCCAAATCT 
GCAGCCTTGC CTAAGGACTC AACTAATGAT CAAACTGTGG AGCTTAAGCA TGACATTTCT 
CAACTCTCAT TCAATGAATG GGATGACTTG GATGATTTTG AAACTCCAGT CAAGAGCAGA 
GTAGCATCCC CAGTTGCAGG GACCTCTACG AAAAAGCCAA GTGTATCAGA CCAGAACACA 
TCCTCAAGCT GCTCTTCTAA GTGTGAGGAG ACTAAAGTAA ATGAGGAGTC ACAAGCTACA 
GAAACCATAA CTGCCCCGAA GGACGTCCTG TCAGCTGCTA ATGGAGTCAG CACAGAGACT 
GCAGAGAGAG AGCCAGAGGA CTCACCCATT AAAAAGTCCA AAAGACCTAA GAAATCAGTC 
CAGCATACAG CATTACTTAG TGACACGGAA GATGAGGAAA TAATCCACTG TGTTTCACCG 
GATACAAACC AGAAGAATTT CAAAAGTGTG GCAGTTGCTG AAAAAGATAA ATGGGGTGAG 
CCAAATGTCA TAGACAGTGA TGACTGTGAG GAGAACAACC ATTATGAAGG TTTTGAGGAT 
TTCATCCCCC CGTCCCCTAT TCCAGAAGAG ATCAGTCTCT CTGTCTCAGA TAAGGAGAAA 
AGTTCATCTG AGCCTGTTAC TCCTGCTAAC AAAAAAGAAT CTTCTCGATC TGCCTCAGGC 
CTGCCAGCAC CCTTGGATCA ATCTGCCAAA GGATTAAAAG GAGCTGATGA CGCTCTCTTT 
AGTGTTATGG AATCTATCTG TTGTCTAGTG GACACTATTC CTGAGCATGA GCTCATAGCG 
CTGACCTGTG GGACTGAGCT TTTACTGCAG AGAGCACACA GGAAAAGGAT TCTTGCTAAA 
GGTGGGTCAT CTAGAACATC TCATTCTGAC TCAGTATCAA CTCCTGGTTT CCTAAACAGA 
CCTACGTTTG GAGTTACACC CTCCAATCTG ACATCCCTGA CCCCGGTAAC TTCAGGAAAA 
AGAGAAGGTG TCAAAACTGG CTTTTCTTTT CGCAAATCCA TTGCCTCTGT CATGTCTGTG 
GGTGATGAGA GTGTTTTTGA GGATTCTGAC TGCATCATAA ATGGAGTCGA AACACCTGGT 
GGTACCTGGA ATCCTAACAG CTCCACTAAA ATATCAGCTG GTAGGGATAC CTTTAACGGG 
TCAATCCAAA CTTTAAGCAA ACCTGAGTCA AAGACAGATA AATGCTACTC AAGGTTGTCC 
TTCAATGAGT CAAGCAATCA GACTGACCTG TTTTATTCCC CCAAGAGAGT GGATTCAGGC 
AGCAGAAATG CTGACAGCAG TGTTGAGATC AACATTGCAG GATCCAGCTC TTTACGAACA 
GGAGCAGAGC CAGTTGATGA TTTTTTACTC GATGACTTCG ATATTGATGA TTTTGATGAG 
AATGATATTC CAGATTATTA TGAAGAACCT CCAAGTGTCT TGGAATCAAG AAATAACTCT 
GGTGTAAAAA CACCATCAGT ACAGGAGGGA GGATCATCAA AATCTTTTGA GAGGAAGACA 
TTCACACCAC CAGCACCAAA GTCTATTAAA ACTCCCAACC CAGAACCTTT GTACAGGAAC 
CCAGCTCATG ACCGCTTCAG AGGCTTTAAC TTTCCTCATA GCCCAGAGAT GATGAAGATC 
TTCCATAAGA AGTTTGGACT TCATCAGTTC CGCTTTAACC AGCTGGAAGC TATTAATGCC 
TCGCTGTTAG GAGAAGACAC ATTTGTATTG ATGCCTACAG GTGGTGGTAA AAGTCTCTGT 
TATCAGCTTC CTGCTTGTGT CTCTGCTGGG GTGACTGTAG TCATTTCTCC TCTACGCTCT 
TTAATAGTTG ACCAAGTACA AAAACTCACC ACGTTAGATA TCTGTGCAAC TAGTTTGTCC 
GGGGACAAAA AAGATAGTGA GGCTGCCCGA ATCTACATGC AGCTGTCCAG GAAAGATCCT 
GCCATCAAAC TGCTTTATGC TACTCCTGAG AAGGTTTGTG CAAGTGGGCG GATGATCAGT 
GCACTTCAGA ATCTGTATGA AAGAGGTCTG CTGGCTCGCT TGGTCATTGA TGAGGCCCAT 
TGCGTCAGTC AGTGGGGTCA TGATTTCAGA CCAGACTACA AGCGCTTGCA TGAGCTCAGG 
CGGATGTTTC CCAATGTTCC TATAATGGCA CTGACGGCCA CAGCCACTCC TAGAGTTCAG 
AAAGACATTC TTAACCAATT GGCAATGACA CGTCCTCAGG TATTTACTAT GAGCTTTAAC 
AGGAACAATC TGAAATACTC AGTTTTGCCT AAGAAGCCCA AGAAGGTTGA TGAGGAATGC 
ATTCAGTGGA TTAAGAAATA TTACCCACGT GACTCTGGGA TTGTGTACTG CTTGTCTCGT 
AATGACTGCG ACACTCTGGC TGACAGTCTT CAGAGGGCAG GGATCGCAGC GCTGGCGTAT 
CATGCTGGCC TAAGTGACAG CGACAGGGAA TATGTCCAGA ACAAGTGGAT CAACCAGGAC 
GGCTGTCAGG TTATGTGCGC AACCATAGCA TTTGGCATGG GAATCGATAA GCCTGATGTC 
CGTTATGTGA TCCATGCGAG TCTCCCCAAA TCAGTGGAGG GTTACTACCA GGAGTCAGGC 
AGAGCAGGGC GAGATGGAGA GATTTCCCAT TGTGTCCTCT TCTATTCCTA CAGTGACGTT 
ATCCGCATCA AGAGACTCAT TGCTATGGAT AAAGATGGCA ACCAGCAATC CAAAGCCACC 
CACATCAATA ACCTGCACAG CATGGTGCAC TTCTGTGAGA ATGTGGCTGA ATGCAGAAGG 
ATCCAGCTGC TGGCTTACTT TGGGGAGCAC ACGTTTAACA CGAGCTTCTG TAAAGAGCAT 
CCTGAGGTCA TCTGTGACAA CTGTGCCAGA CCAAACAAAT ATAAATCCAG AAATGTGACC 
GATGACGTTA AGAAGATTGC GAGGTTTGTG CAGGACAACT GTGAGAAGGT TGGAAACAGA 
TACGGCAAAT CCGCCCAGCA GAACAGGCTC ACTCTCAACA TGCTGGTTGA AATATTTCTG 
GGCTCCAAAA GCGCTCGCAT CCAGACTGGG ATGTTCGGAG TTGGAGCAGC TTATTCCAAA 
CACAACGCTG AGAGGCTCTT TAAGAAGCTG GTGCTGGATA ATGTTCTGAT GGAGGATCTG 
TACATCACTA ACAGTGGCCA GGCTGTGGCC TATATCTCTG CAGGCCCTAA AGCTATGAGT 
GTACTGAATG GTTGCATGCA GGTTGAATTT CATGAGACCG AGAGCGCATC CAGCATCAGA 
AAGCACAGAG CGTCTGTGTG TGAGAAGGTG TCTAAGAGAG AGGAGATGGT GAAAAAGTGT 
CTGGGTGAAC TTAATGATCT ATGCAAGAAG TTGGGCAAAG TCTTCGGCAT TCATTACTAC 
AATATCTTCT CCACCGCTAC ACTCAAAAAG ATTGCTGAGA CTCTCTCGGC TGATCCGGAG 
GTTCTGCTGC AGATTGATGG TGTAACTGAA GACAAACTGG AGAAATATGG AGCTGAGTTT 
ATTGAACTGC TACAGAAATA CTCTGAGTGG CAGCTACCTG CTGAAGCACA GGCTGAGAGT 
TCAGGCTGGA TCGACACGAC CAGAGGCCAT CAAAATGATG AAGACGACAA CGATGATGAT 
GATGAAGGAG GTGGGGATGT CACATCGACA TATTTCAGAA GCAACTCTGG ACGAGGAGCC 
AAGAGAAAGC AAGGCTCTTA CTCCAGAAAA CCCAAACGAA GAAAAGGCTC CAGTGGCCAG 
AACTCCTCTG CAAAAGGTGG ATATAGCAGT AACTGGTCTT CATCACGAGG AGGAGGAAGA 
GGAGGTGGAT ACAGGGGTGG CAGTCGAGGA GCAGGCCGAG GCTCCAGATC TGCTCCTTCA 
GGCTCAGCAG CAAAAAGGCC GGGCTTCATG TCGTTACCAA CACCGCAGGC TGCTGCTCGT 
CCATTCCTCA AACCGTCCTT CTCACACCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( XM_017352049.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207538.1 Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa55902
Clone ID Related Accession (Same CDS sequence)	XM_017352049.2 , XM_017352049.1
Accession Version	XM_017352049.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Bloom syndrome protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAATG AGAGAGCGAG TTGCAAATAC GCGATGTCGA GTCTTCCTCA AAACAACCTG 
AAGGAGCATC TGGAGCGACA CAACAATGCT GCCCAGAACA AGCTGTCACT GCTCAAACCA 
AAGCCTGGGG GGTTTTGCTT CAAAAAGAAG TCTTCGTCAG GCATCTCCAA AGTGGAAGTT 
CCTCAAAAGG TAACTGGTTC AAGTGTCTTA GCAAACAGGA GTGTCAATAT TCCCAGCAAC 
TTTGAAGTAA CCAAAAATCC TGTGACGTTT TCGCAAAAGC CAGAGAGAGT GGCACCGAAA 
GTCAACTTCT TCACTGCGCC CAGCAAACCG AAGACAAATA CAGTTAATCC ACTAGCAAAC 
CCATTCGCTT TGAACAAAAC CCCTCTTGTC CAATCTACCA TTAAGGCTTT GCCCAAATCT 
GCAGCCTTGC CTAAGGACTC AACTAATGAT CAAACTGTGG AGCTTAAGCA TGACATTTCT 
CAACTCTCAT TCAATGAATG GGATGACTTG GATGATTTTG AAACTCCAGT CAAGAGCAGA 
GTAGCATCCC CAGTTGCAGG GACCTCTACG AAAAAGCCAA GTGTATCAGA CCAGAACACA 
TCCTCAAGCT GCTCTTCTAA GTGTGAGGAG ACTAAAGTAA ATGAGGAGTC ACAAGCTACA 
GAAACCATAA CTGCCCCGAA GGACGTCCTG TCAGCTGCTA ATGGAGTCAG CACAGAGACT 
GCAGAGAGAG AGCCAGAGGA CTCACCCATT AAAAAGTCCA AAAGACCTAA GAAATCAGTC 
CAGCATACAG CATTACTTAG TGACACGGAA GATGAGGAAA TAATCCACTG TGTTTCACCG 
GATACAAACC AGAAGAATTT CAAAAGTGTG GCAGTTGCTG AAAAAGATAA ATGGGGTGAG 
CCAAATGTCA TAGACAGTGA TGACTGTGAG GAGAACAACC ATTATGAAGG TTTTGAGGAT 
TTCATCCCCC CGTCCCCTAT TCCAGAAGAG ATCAGTCTCT CTGTCTCAGA TAAGGAGAAA 
AGTTCATCTG AGCCTGTTAC TCCTGCTAAC AAAAAAGAAT CTTCTCGATC TGCCTCAGGC 
CTGCCAGCAC CCTTGGATCA ATCTGCCAAA GGATTAAAAG GAGCTGATGA CGCTCTCTTT 
AGTGTTATGG AATCTATCTG TTGTCTAGTG GACACTATTC CTGAGCATGA GCTCATAGCG 
CTGACCTGTG GGACTGAGCT TTTACTGCAG AGAGCACACA GGAAAAGGAT TCTTGCTAAA 
GGTGGGTCAT CTAGAACATC TCATTCTGAC TCAGTATCAA CTCCTGGTTT CCTAAACAGA 
CCTACGTTTG GAGTTACACC CTCCAATCTG ACATCCCTGA CCCCGGTAAC TTCAGGAAAA 
AGAGAAGGTG TCAAAACTGG CTTTTCTTTT CGCAAATCCA TTGCCTCTGT CATGTCTGTG 
GGTGATGAGA GTGTTTTTGA GGATTCTGAC TGCATCATAA ATGGAGTCGA AACACCTGGT 
GGTACCTGGA ATCCTAACAG CTCCACTAAA ATATCAGCTG GTAGGGATAC CTTTAACGGG 
TCAATCCAAA CTTTAAGCAA ACCTGAGTCA AAGACAGATA AATGCTACTC AAGGTTGTCC 
TTCAATGAGT CAAGCAATCA GACTGACCTG TTTTATTCCC CCAAGAGAGT GGATTCAGGC 
AGCAGAAATG CTGACAGCAG TGTTGAGATC AACATTGCAG GATCCAGCTC TTTACGAACA 
GGAGCAGAGC CAGTTGATGA TTTTTTACTC GATGACTTCG ATATTGATGA TTTTGATGAG 
AATGATATTC CAGATTATTA TGAAGAACCT CCAAGTGTCT TGGAATCAAG AAATAACTCT 
GGTGTAAAAA CACCATCAGT ACAGGAGGGA GGATCATCAA AATCTTTTGA GAGGAAGACA 
TTCACACCAC CAGCACCAAA GTCTATTAAA ACTCCCAACC CAGAACCTTT GTACAGGAAC 
CCAGCTCATG ACCGCTTCAG AGGCTTTAAC TTTCCTCATA GCCCAGAGAT GATGAAGATC 
TTCCATAAGA AGTTTGGACT TCATCAGTTC CGCTTTAACC AGCTGGAAGC TATTAATGCC 
TCGCTGTTAG GAGAAGACAC ATTTGTATTG ATGCCTACAG GTGGTGGTAA AAGTCTCTGT 
TATCAGCTTC CTGCTTGTGT CTCTGCTGGG GTGACTGTAG TCATTTCTCC TCTACGCTCT 
TTAATAGTTG ACCAAGTACA AAAACTCACC ACGTTAGATA TCTGTGCAAC TAGTTTGTCC 
GGGGACAAAA AAGATAGTGA GGCTGCCCGA ATCTACATGC AGCTGTCCAG GAAAGATCCT 
GCCATCAAAC TGCTTTATGC TACTCCTGAG AAGGTTTGTG CAAGTGGGCG GATGATCAGT 
GCACTTCAGA ATCTGTATGA AAGAGGTCTG CTGGCTCGCT TGGTCATTGA TGAGGCCCAT 
TGCGTCAGTC AGTGGGGTCA TGATTTCAGA CCAGACTACA AGCGCTTGCA TGAGCTCAGG 
CGGATGTTTC CCAATGTTCC TATAATGGCA CTGACGGCCA CAGCCACTCC TAGAGTTCAG 
AAAGACATTC TTAACCAATT GGCAATGACA CGTCCTCAGG TATTTACTAT GAGCTTTAAC 
AGGAACAATC TGAAATACTC AGTTTTGCCT AAGAAGCCCA AGAAGGTTGA TGAGGAATGC 
ATTCAGTGGA TTAAGAAATA TTACCCACGT GACTCTGGGA TTGTGTACTG CTTGTCTCGT 
AATGACTGCG ACACTCTGGC TGACAGTCTT CAGAGGGCAG GGATCGCAGC GCTGGCGTAT 
CATGCTGGCC TAAGTGACAG CGACAGGGAA TATGTCCAGA ACAAGTGGAT CAACCAGGAC 
GGCTGTCAGG TTATGTGCGC AACCATAGCA TTTGGCATGG GAATCGATAA GCCTGATGTC 
CGTTATGTGA TCCATGCGAG TCTCCCCAAA TCAGTGGAGG GTTACTACCA GGAGTCAGGC 
AGAGCAGGGC GAGATGGAGA GATTTCCCAT TGTGTCCTCT TCTATTCCTA CAGTGACGTT 
ATCCGCATCA AGAGACTCAT TGCTATGGAT AAAGATGGCA ACCAGCAATC CAAAGCCACC 
CACATCAATA ACCTGCACAG CATGGTGCAC TTCTGTGAGA ATGTGGCTGA ATGCAGAAGG 
ATCCAGCTGC TGGCTTACTT TGGGGAGCAC ACGTTTAACA CGAGCTTCTG TAAAGAGCAT 
CCTGAGGTCA TCTGTGACAA CTGTGCCAGA CCAAACAAAT ATAAATCCAG AAATGTGACC 
GATGACGTTA AGAAGATTGC GAGGTTTGTG CAGGACAACT GTGAGAAGGT TGGAAACAGA 
TACGGCAAAT CCGCCCAGCA GAACAGGCTC ACTCTCAACA TGCTGGTTGA AATATTTCTG 
GGCTCCAAAA GCGCTCGCAT CCAGACTGGG ATGTTCGGAG TTGGAGCAGC TTATTCCAAA 
CACAACGCTG AGAGGCTCTT TAAGAAGCTG GTGCTGGATA ATGTTCTGAT GGAGGATCTG 
TACATCACTA ACAGTGGCCA GGCTGTGGCC TATATCTCTG CAGGCCCTAA AGCTATGAGT 
GTACTGAATG GTTGCATGCA GGTTGAATTT CATGAGACCG AGAGCGCATC CAGCATCAGA 
AAGCACAGAG CGTCTGTGTG TGAGAAGGTG TCTAAGAGAG AGGAGATGGT GAAAAAGTGT 
CTGGGTGAAC TTAATGATCT ATGCAAGAAG TTGGGCAAAG TCTTCGGCAT TCATTACTAC 
AATATCTTCT CCACCGCTAC ACTCAAAAAG ATTGCTGAGA CTCTCTCGGC TGATCCGGAG 
GTTCTGCTGC AGATTGATGG TGTAACTGAA GACAAACTGG AGAAATATGG AGCTGAGTTT 
ATTGAACTGC TACAGAAATA CTCTGAGTGG CAGCTACCTG CTGAAGCACA GGCTGAGAGT 
TCAGGCTGGA TCGACACGAC CAGAGGCCAT CAAAATGATG AAGACGACAA CGATGATGAT 
GATGAAGGAG GTGGGGATGT CACATCGACA TATTTCAGAA GCAACTCTGG ACGAGGAGCC 
AAGAGAAAGC AAGGCTCTTA CTCCAGAAAA CCCAAACGAA GAAAAGGCTC CAGTGGCCAG 
AACTCCTCTG CAAAAGGTGG ATATAGCAGT AACTGGTCTT CATCACGAGG AGGAGGAAGA 
GGAGGTGGAT ACAGGGGTGG CAGTCGAGGA GCAGGCCGAG GCTCCAGATC TGCTCCTTCA 
GGCTCAGCAG CAAAAAGGCC GGGCTTCATG TCGTTACCAA CACCGCAGGC TGCTGCTCGT 
CCATTCCTCA AACCGTCCTT CTCACACCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207538.1
CDS	477..4829
Translation	MTNERASCKYAMSSLPQNNLKEHLERHNNAAQNKLSLLKPKPGGFCFKKKSSSGISKVEVPQKVTGSSVLANRSVNIPSNFEVTKNPVTFSQKPERVAPKVNFFTAPSKPKTNTVNPLANPFALNKTPLVQSTIKALPKSAALPKDSTNDQTVELKHDISQLSFNEWDDLDDFETPVKSRVASPVAGTSTKKPSVSDQNTSSSCSSKCEETKVNEESQATETITAPKDVLSAANGVSTETAEREPEDSPIKKSKRPKKSVQHTALLSDTEDEEIIHCVSPDTNQKNFKSVAVAEKDKWGEPNVIDSDDCEENNHYEGFEDFIPPSPIPEEISLSVSDKEKSSSEPVTPANKKESSRSASGLPAPLDQSAKGLKGADDALFSVMESICCLVDTIPEHELIALTCGTELLLQRAHRKRILAKGGSSRTSHSDSVSTPGFLNRPTFGVTPSNLTSLTPVTSGKREGVKTGFSFRKSIASVMSVGDESVFEDSDCIINGVETPGGTWNPNSSTKISAGRDTFNGSIQTLSKPESKTDKCYSRLSFNESSNQTDLFYSPKRVDSGSRNADSSVEINIAGSSSLRTGAEPVDDFLLDDFDIDDFDENDIPDYYEEPPSVLESRNNSGVKTPSVQEGGSSKSFERKTFTPPAPKSIKTPNPEPLYRNPAHDRFRGFNFPHSPEMMKIFHKKFGLHQFRFNQLEAINASLLGEDTFVLMPTGGGKSLCYQLPACVSAGVTVVISPLRSLIVDQVQKLTTLDICATSLSGDKKDSEAARIYMQLSRKDPAIKLLYATPEKVCASGRMISALQNLYERGLLARLVIDEAHCVSQWGHDFRPDYKRLHELRRMFPNVPIMALTATATPRVQKDILNQLAMTRPQVFTMSFNRNNLKYSVLPKKPKKVDEECIQWIKKYYPRDSGIVYCLSRNDCDTLADSLQRAGIAALAYHAGLSDSDREYVQNKWINQDGCQVMCATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCVLFYSYSDVIRIKRLIAMDKDGNQQSKATHINNLHSMVHFCENVAECRRIQLLAYFGEHTFNTSFCKEHPEVICDNCARPNKYKSRNVTDDVKKIARFVQDNCEKVGNRYGKSAQQNRLTLNMLVEIFLGSKSARIQTGMFGVGAAYSKHNAERLFKKLVLDNVLMEDLYITNSGQAVAYISAGPKAMSVLNGCMQVEFHETESASSIRKHRASVCEKVSKREEMVKKCLGELNDLCKKLGKVFGIHYYNIFSTATLKKIAETLSADPEVLLQIDGVTEDKLEKYGAEFIELLQKYSEWQLPAEAQAESSGWIDTTRGHQNDEDDNDDDDEGGGDVTSTYFRSNSGRGAKRKQGSYSRKPKRRKGSSGQNSSAKGGYSSNWSSSRGGGRGGGYRGGSRGAGRGSRSAPSGSAAKRPGFMSLPTPQAAARPFLKPSFSHL

Target ORF information:

RefSeq Version	XM_017352049.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352049.1

ORF Insert Sequence:

ATGACCAATG AGAGAGCGAG TTGCAAATAC GCGATGTCGA GTCTTCCTCA AAACAACCTG 
AAGGAGCATC TGGAGCGACA CAACAATGCT GCCCAGAACA AGCTGTCACT GCTCAAACCA 
AAGCCTGGGG GGTTTTGCTT CAAAAAGAAG TCTTCGTCAG GCATCTCCAA AGTGGAAGTT 
CCTCAAAAGG TAACTGGTTC AAGTGTCTTA GCAAACAGGA GTGTCAATAT TCCCAGCAAC 
TTTGAAGTAA CCAAAAATCC TGTGACGTTT TCGCAAAAGC CAGAGAGAGT GGCACCGAAA 
GTCAACTTCT TCACTGCGCC CAGCAAACCG AAGACAAATA CAGTTAATCC ACTAGCAAAC 
CCATTCGCTT TGAACAAAAC CCCTCTTGTC CAATCTACCA TTAAGGCTTT GCCCAAATCT 
GCAGCCTTGC CTAAGGACTC AACTAATGAT CAAACTGTGG AGCTTAAGCA TGACATTTCT 
CAACTCTCAT TCAATGAATG GGATGACTTG GATGATTTTG AAACTCCAGT CAAGAGCAGA 
GTAGCATCCC CAGTTGCAGG GACCTCTACG AAAAAGCCAA GTGTATCAGA CCAGAACACA 
TCCTCAAGCT GCTCTTCTAA GTGTGAGGAG ACTAAAGTAA ATGAGGAGTC ACAAGCTACA 
GAAACCATAA CTGCCCCGAA GGACGTCCTG TCAGCTGCTA ATGGAGTCAG CACAGAGACT 
GCAGAGAGAG AGCCAGAGGA CTCACCCATT AAAAAGTCCA AAAGACCTAA GAAATCAGTC 
CAGCATACAG CATTACTTAG TGACACGGAA GATGAGGAAA TAATCCACTG TGTTTCACCG 
GATACAAACC AGAAGAATTT CAAAAGTGTG GCAGTTGCTG AAAAAGATAA ATGGGGTGAG 
CCAAATGTCA TAGACAGTGA TGACTGTGAG GAGAACAACC ATTATGAAGG TTTTGAGGAT 
TTCATCCCCC CGTCCCCTAT TCCAGAAGAG ATCAGTCTCT CTGTCTCAGA TAAGGAGAAA 
AGTTCATCTG AGCCTGTTAC TCCTGCTAAC AAAAAAGAAT CTTCTCGATC TGCCTCAGGC 
CTGCCAGCAC CCTTGGATCA ATCTGCCAAA GGATTAAAAG GAGCTGATGA CGCTCTCTTT 
AGTGTTATGG AATCTATCTG TTGTCTAGTG GACACTATTC CTGAGCATGA GCTCATAGCG 
CTGACCTGTG GGACTGAGCT TTTACTGCAG AGAGCACACA GGAAAAGGAT TCTTGCTAAA 
GGTGGGTCAT CTAGAACATC TCATTCTGAC TCAGTATCAA CTCCTGGTTT CCTAAACAGA 
CCTACGTTTG GAGTTACACC CTCCAATCTG ACATCCCTGA CCCCGGTAAC TTCAGGAAAA 
AGAGAAGGTG TCAAAACTGG CTTTTCTTTT CGCAAATCCA TTGCCTCTGT CATGTCTGTG 
GGTGATGAGA GTGTTTTTGA GGATTCTGAC TGCATCATAA ATGGAGTCGA AACACCTGGT 
GGTACCTGGA ATCCTAACAG CTCCACTAAA ATATCAGCTG GTAGGGATAC CTTTAACGGG 
TCAATCCAAA CTTTAAGCAA ACCTGAGTCA AAGACAGATA AATGCTACTC AAGGTTGTCC 
TTCAATGAGT CAAGCAATCA GACTGACCTG TTTTATTCCC CCAAGAGAGT GGATTCAGGC 
AGCAGAAATG CTGACAGCAG TGTTGAGATC AACATTGCAG GATCCAGCTC TTTACGAACA 
GGAGCAGAGC CAGTTGATGA TTTTTTACTC GATGACTTCG ATATTGATGA TTTTGATGAG 
AATGATATTC CAGATTATTA TGAAGAACCT CCAAGTGTCT TGGAATCAAG AAATAACTCT 
GGTGTAAAAA CACCATCAGT ACAGGAGGGA GGATCATCAA AATCTTTTGA GAGGAAGACA 
TTCACACCAC CAGCACCAAA GTCTATTAAA ACTCCCAACC CAGAACCTTT GTACAGGAAC 
CCAGCTCATG ACCGCTTCAG AGGCTTTAAC TTTCCTCATA GCCCAGAGAT GATGAAGATC 
TTCCATAAGA AGTTTGGACT TCATCAGTTC CGCTTTAACC AGCTGGAAGC TATTAATGCC 
TCGCTGTTAG GAGAAGACAC ATTTGTATTG ATGCCTACAG GTGGTGGTAA AAGTCTCTGT 
TATCAGCTTC CTGCTTGTGT CTCTGCTGGG GTGACTGTAG TCATTTCTCC TCTACGCTCT 
TTAATAGTTG ACCAAGTACA AAAACTCACC ACGTTAGATA TCTGTGCAAC TAGTTTGTCC 
GGGGACAAAA AAGATAGTGA GGCTGCCCGA ATCTACATGC AGCTGTCCAG GAAAGATCCT 
GCCATCAAAC TGCTTTATGC TACTCCTGAG AAGGTTTGTG CAAGTGGGCG GATGATCAGT 
GCACTTCAGA ATCTGTATGA AAGAGGTCTG CTGGCTCGCT TGGTCATTGA TGAGGCCCAT 
TGCGTCAGTC AGTGGGGTCA TGATTTCAGA CCAGACTACA AGCGCTTGCA TGAGCTCAGG 
CGGATGTTTC CCAATGTTCC TATAATGGCA CTGACGGCCA CAGCCACTCC TAGAGTTCAG 
AAAGACATTC TTAACCAATT GGCAATGACA CGTCCTCAGG TATTTACTAT GAGCTTTAAC 
AGGAACAATC TGAAATACTC AGTTTTGCCT AAGAAGCCCA AGAAGGTTGA TGAGGAATGC 
ATTCAGTGGA TTAAGAAATA TTACCCACGT GACTCTGGGA TTGTGTACTG CTTGTCTCGT 
AATGACTGCG ACACTCTGGC TGACAGTCTT CAGAGGGCAG GGATCGCAGC GCTGGCGTAT 
CATGCTGGCC TAAGTGACAG CGACAGGGAA TATGTCCAGA ACAAGTGGAT CAACCAGGAC 
GGCTGTCAGG TTATGTGCGC AACCATAGCA TTTGGCATGG GAATCGATAA GCCTGATGTC 
CGTTATGTGA TCCATGCGAG TCTCCCCAAA TCAGTGGAGG GTTACTACCA GGAGTCAGGC 
AGAGCAGGGC GAGATGGAGA GATTTCCCAT TGTGTCCTCT TCTATTCCTA CAGTGACGTT 
ATCCGCATCA AGAGACTCAT TGCTATGGAT AAAGATGGCA ACCAGCAATC CAAAGCCACC 
CACATCAATA ACCTGCACAG CATGGTGCAC TTCTGTGAGA ATGTGGCTGA ATGCAGAAGG 
ATCCAGCTGC TGGCTTACTT TGGGGAGCAC ACGTTTAACA CGAGCTTCTG TAAAGAGCAT 
CCTGAGGTCA TCTGTGACAA CTGTGCCAGA CCAAACAAAT ATAAATCCAG AAATGTGACC 
GATGACGTTA AGAAGATTGC GAGGTTTGTG CAGGACAACT GTGAGAAGGT TGGAAACAGA 
TACGGCAAAT CCGCCCAGCA GAACAGGCTC ACTCTCAACA TGCTGGTTGA AATATTTCTG 
GGCTCCAAAA GCGCTCGCAT CCAGACTGGG ATGTTCGGAG TTGGAGCAGC TTATTCCAAA 
CACAACGCTG AGAGGCTCTT TAAGAAGCTG GTGCTGGATA ATGTTCTGAT GGAGGATCTG 
TACATCACTA ACAGTGGCCA GGCTGTGGCC TATATCTCTG CAGGCCCTAA AGCTATGAGT 
GTACTGAATG GTTGCATGCA GGTTGAATTT CATGAGACCG AGAGCGCATC CAGCATCAGA 
AAGCACAGAG CGTCTGTGTG TGAGAAGGTG TCTAAGAGAG AGGAGATGGT GAAAAAGTGT 
CTGGGTGAAC TTAATGATCT ATGCAAGAAG TTGGGCAAAG TCTTCGGCAT TCATTACTAC 
AATATCTTCT CCACCGCTAC ACTCAAAAAG ATTGCTGAGA CTCTCTCGGC TGATCCGGAG 
GTTCTGCTGC AGATTGATGG TGTAACTGAA GACAAACTGG AGAAATATGG AGCTGAGTTT 
ATTGAACTGC TACAGAAATA CTCTGAGTGG CAGCTACCTG CTGAAGCACA GGCTGAGAGT 
TCAGGCTGGA TCGACACGAC CAGAGGCCAT CAAAATGATG AAGACGACAA CGATGATGAT 
GATGAAGGAG GTGGGGATGT CACATCGACA TATTTCAGAA GCAACTCTGG ACGAGGAGCC 
AAGAGAAAGC AAGGCTCTTA CTCCAGAAAA CCCAAACGAA GAAAAGGCTC CAGTGGCCAG 
AACTCCTCTG CAAAAGGTGG ATATAGCAGT AACTGGTCTT CATCACGAGG AGGAGGAAGA 
GGAGGTGGAT ACAGGGGTGG CAGTCGAGGA GCAGGCCGAG GCTCCAGATC TGCTCCTTCA 
GGCTCAGCAG CAAAAAGGCC GGGCTTCATG TCGTTACCAA CACCGCAGGC TGCTGCTCGT 
CCATTCCTCA AACCGTCCTT CTCACACCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( XM_021467812.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021323487.1 Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X2, mRNA.

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CloneID	ODa55903
Clone ID Related Accession (Same CDS sequence)	XM_021467812.1 , XM_017352050.1 , XM_017352050.2
Accession Version	XM_021467812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Bloom syndrome protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGTC TTCCTCAAAA CAACCTGAAG GAGCATCTGG AGCGACACAA CAATGCTGCC 
CAGAACAAGC TGTCACTGCT CAAACCAAAG CCTGGGGGGT TTTGCTTCAA AAAGAAGTCT 
TCGTCAGGCA TCTCCAAAGT GGAAGTTCCT CAAAAGGTAA CTGGTTCAAG TGTCTTAGCA 
AACAGGAGTG TCAATATTCC CAGCAACTTT GAAGTAACCA AAAATCCTGT GACGTTTTCG 
CAAAAGCCAG AGAGAGTGGC ACCGAAAGTC AACTTCTTCA CTGCGCCCAG CAAACCGAAG 
ACAAATACAG TTAATCCACT AGCAAACCCA TTCGCTTTGA ACAAAACCCC TCTTGTCCAA 
TCTACCATTA AGGCTTTGCC CAAATCTGCA GCCTTGCCTA AGGACTCAAC TAATGATCAA 
ACTGTGGAGC TTAAGCATGA CATTTCTCAA CTCTCATTCA ATGAATGGGA TGACTTGGAT 
GATTTTGAAA CTCCAGTCAA GAGCAGAGTA GCATCCCCAG TTGCAGGGAC CTCTACGAAA 
AAGCCAAGTG TATCAGACCA GAACACATCC TCAAGCTGCT CTTCTAAGTG TGAGGAGACT 
AAAGTAAATG AGGAGTCACA AGCTACAGAA ACCATAACTG CCCCGAAGGA CGTCCTGTCA 
GCTGCTAATG GAGTCAGCAC AGAGACTGCA GAGAGAGAGC CAGAGGACTC ACCCATTAAA 
AAGTCCAAAA GACCTAAGAA ATCAGTCCAG CATACAGCAT TACTTAGTGA CACGGAAGAT 
GAGGAAATAA TCCACTGTGT TTCACCGGAT ACAAACCAGA AGAATTTCAA AAGTGTGGCA 
GTTGCTGAAA AAGATAAATG GGGTGAGCCA AATGTCATAG ACAGTGATGA CTGTGAGGAG 
AACAACCATT ATGAAGGTTT TGAGGATTTC ATCCCCCCGT CCCCTATTCC AGAAGAGATC 
AGTCTCTCTG TCTCAGATAA GGAGAAAAGT TCATCTGAGC CTGTTACTCC TGCTAACAAA 
AAAGAATCTT CTCGATCTGC CTCAGGCCTG CCAGCACCCT TGGATCAATC TGCCAAAGGA 
TTAAAAGGAG CTGATGACGC TCTCTTTAGT GTTATGGAAT CTATCTGTTG TCTAGTGGAC 
ACTATTCCTG AGCATGAGCT CATAGCGCTG ACCTGTGGGA CTGAGCTTTT ACTGCAGAGA 
GCACACAGGA AAAGGATTCT TGCTAAAGGT GGGTCATCTA GAACATCTCA TTCTGACTCA 
GTATCAACTC CTGGTTTCCT AAACAGACCT ACGTTTGGAG TTACACCCTC CAATCTGACA 
TCCCTGACCC CGGTAACTTC AGGAAAAAGA GAAGGTGTCA AAACTGGCTT TTCTTTTCGC 
AAATCCATTG CCTCTGTCAT GTCTGTGGGT GATGAGAGTG TTTTTGAGGA TTCTGACTGC 
ATCATAAATG GAGTCGAAAC ACCTGGTGGT ACCTGGAATC CTAACAGCTC CACTAAAATA 
TCAGCTGGTA GGGATACCTT TAACGGGTCA ATCCAAACTT TAAGCAAACC TGAGTCAAAG 
ACAGATAAAT GCTACTCAAG GTTGTCCTTC AATGAGTCAA GCAATCAGAC TGACCTGTTT 
TATTCCCCCA AGAGAGTGGA TTCAGGCAGC AGAAATGCTG ACAGCAGTGT TGAGATCAAC 
ATTGCAGGAT CCAGCTCTTT ACGAACAGGA GCAGAGCCAG TTGATGATTT TTTACTCGAT 
GACTTCGATA TTGATGATTT TGATGAGAAT GATATTCCAG ATTATTATGA AGAACCTCCA 
AGTGTCTTGG AATCAAGAAA TAACTCTGGT GTAAAAACAC CATCAGTACA GGAGGGAGGA 
TCATCAAAAT CTTTTGAGAG GAAGACATTC ACACCACCAG CACCAAAGTC TATTAAAACT 
CCCAACCCAG AACCTTTGTA CAGGAACCCA GCTCATGACC GCTTCAGAGG CTTTAACTTT 
CCTCATAGCC CAGAGATGAT GAAGATCTTC CATAAGAAGT TTGGACTTCA TCAGTTCCGC 
TTTAACCAGC TGGAAGCTAT TAATGCCTCG CTGTTAGGAG AAGACACATT TGTATTGATG 
CCTACAGGTG GTGGTAAAAG TCTCTGTTAT CAGCTTCCTG CTTGTGTCTC TGCTGGGGTG 
ACTGTAGTCA TTTCTCCTCT ACGCTCTTTA ATAGTTGACC AAGTACAAAA ACTCACCACG 
TTAGATATCT GTGCAACTAG TTTGTCCGGG GACAAAAAAG ATAGTGAGGC TGCCCGAATC 
TACATGCAGC TGTCCAGGAA AGATCCTGCC ATCAAACTGC TTTATGCTAC TCCTGAGAAG 
GTTTGTGCAA GTGGGCGGAT GATCAGTGCA CTTCAGAATC TGTATGAAAG AGGTCTGCTG 
GCTCGCTTGG TCATTGATGA GGCCCATTGC GTCAGTCAGT GGGGTCATGA TTTCAGACCA 
GACTACAAGC GCTTGCATGA GCTCAGGCGG ATGTTTCCCA ATGTTCCTAT AATGGCACTG 
ACGGCCACAG CCACTCCTAG AGTTCAGAAA GACATTCTTA ACCAATTGGC AATGACACGT 
CCTCAGGTAT TTACTATGAG CTTTAACAGG AACAATCTGA AATACTCAGT TTTGCCTAAG 
AAGCCCAAGA AGGTTGATGA GGAATGCATT CAGTGGATTA AGAAATATTA CCCACGTGAC 
TCTGGGATTG TGTACTGCTT GTCTCGTAAT GACTGCGACA CTCTGGCTGA CAGTCTTCAG 
AGGGCAGGGA TCGCAGCGCT GGCGTATCAT GCTGGCCTAA GTGACAGCGA CAGGGAATAT 
GTCCAGAACA AGTGGATCAA CCAGGACGGC TGTCAGGTTA TGTGCGCAAC CATAGCATTT 
GGCATGGGAA TCGATAAGCC TGATGTCCGT TATGTGATCC ATGCGAGTCT CCCCAAATCA 
GTGGAGGGTT ACTACCAGGA GTCAGGCAGA GCAGGGCGAG ATGGAGAGAT TTCCCATTGT 
GTCCTCTTCT ATTCCTACAG TGACGTTATC CGCATCAAGA GACTCATTGC TATGGATAAA 
GATGGCAACC AGCAATCCAA AGCCACCCAC ATCAATAACC TGCACAGCAT GGTGCACTTC 
TGTGAGAATG TGGCTGAATG CAGAAGGATC CAGCTGCTGG CTTACTTTGG GGAGCACACG 
TTTAACACGA GCTTCTGTAA AGAGCATCCT GAGGTCATCT GTGACAACTG TGCCAGACCA 
AACAAATATA AATCCAGAAA TGTGACCGAT GACGTTAAGA AGATTGCGAG GTTTGTGCAG 
GACAACTGTG AGAAGGTTGG AAACAGATAC GGCAAATCCG CCCAGCAGAA CAGGCTCACT 
CTCAACATGC TGGTTGAAAT ATTTCTGGGC TCCAAAAGCG CTCGCATCCA GACTGGGATG 
TTCGGAGTTG GAGCAGCTTA TTCCAAACAC AACGCTGAGA GGCTCTTTAA GAAGCTGGTG 
CTGGATAATG TTCTGATGGA GGATCTGTAC ATCACTAACA GTGGCCAGGC TGTGGCCTAT 
ATCTCTGCAG GCCCTAAAGC TATGAGTGTA CTGAATGGTT GCATGCAGGT TGAATTTCAT 
GAGACCGAGA GCGCATCCAG CATCAGAAAG CACAGAGCGT CTGTGTGTGA GAAGGTGTCT 
AAGAGAGAGG AGATGGTGAA AAAGTGTCTG GGTGAACTTA ATGATCTATG CAAGAAGTTG 
GGCAAAGTCT TCGGCATTCA TTACTACAAT ATCTTCTCCA CCGCTACACT CAAAAAGATT 
GCTGAGACTC TCTCGGCTGA TCCGGAGGTT CTGCTGCAGA TTGATGGTGT AACTGAAGAC 
AAACTGGAGA AATATGGAGC TGAGTTTATT GAACTGCTAC AGAAATACTC TGAGTGGCAG 
CTACCTGCTG AAGCACAGGC TGAGAGTTCA GGCTGGATCG ACACGACCAG AGGCCATCAA 
AATGATGAAG ACGACAACGA TGATGATGAT GAAGGAGGTG GGGATGTCAC ATCGACATAT 
TTCAGAAGCA ACTCTGGACG AGGAGCCAAG AGAAAGCAAG GCTCTTACTC CAGAAAACCC 
AAACGAAGAA AAGGCTCCAG TGGCCAGAAC TCCTCTGCAA AAGGTGGATA TAGCAGTAAC 
TGGTCTTCAT CACGAGGAGG AGGAAGAGGA GGTGGATACA GGGGTGGCAG TCGAGGAGCA 
GGCCGAGGCT CCAGATCTGC TCCTTCAGGC TCAGCAGCAA AAAGGCCGGG CTTCATGTCG 
TTACCAACAC CGCAGGCTGC TGCTCGTCCA TTCCTCAAAC CGTCCTTCTC ACACCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323487.1
CDS	268..4587
Translation	MSSLPQNNLKEHLERHNNAAQNKLSLLKPKPGGFCFKKKSSSGISKVEVPQKVTGSSVLANRSVNIPSNFEVTKNPVTFSQKPERVAPKVNFFTAPSKPKTNTVNPLANPFALNKTPLVQSTIKALPKSAALPKDSTNDQTVELKHDISQLSFNEWDDLDDFETPVKSRVASPVAGTSTKKPSVSDQNTSSSCSSKCEETKVNEESQATETITAPKDVLSAANGVSTETAEREPEDSPIKKSKRPKKSVQHTALLSDTEDEEIIHCVSPDTNQKNFKSVAVAEKDKWGEPNVIDSDDCEENNHYEGFEDFIPPSPIPEEISLSVSDKEKSSSEPVTPANKKESSRSASGLPAPLDQSAKGLKGADDALFSVMESICCLVDTIPEHELIALTCGTELLLQRAHRKRILAKGGSSRTSHSDSVSTPGFLNRPTFGVTPSNLTSLTPVTSGKREGVKTGFSFRKSIASVMSVGDESVFEDSDCIINGVETPGGTWNPNSSTKISAGRDTFNGSIQTLSKPESKTDKCYSRLSFNESSNQTDLFYSPKRVDSGSRNADSSVEINIAGSSSLRTGAEPVDDFLLDDFDIDDFDENDIPDYYEEPPSVLESRNNSGVKTPSVQEGGSSKSFERKTFTPPAPKSIKTPNPEPLYRNPAHDRFRGFNFPHSPEMMKIFHKKFGLHQFRFNQLEAINASLLGEDTFVLMPTGGGKSLCYQLPACVSAGVTVVISPLRSLIVDQVQKLTTLDICATSLSGDKKDSEAARIYMQLSRKDPAIKLLYATPEKVCASGRMISALQNLYERGLLARLVIDEAHCVSQWGHDFRPDYKRLHELRRMFPNVPIMALTATATPRVQKDILNQLAMTRPQVFTMSFNRNNLKYSVLPKKPKKVDEECIQWIKKYYPRDSGIVYCLSRNDCDTLADSLQRAGIAALAYHAGLSDSDREYVQNKWINQDGCQVMCATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCVLFYSYSDVIRIKRLIAMDKDGNQQSKATHINNLHSMVHFCENVAECRRIQLLAYFGEHTFNTSFCKEHPEVICDNCARPNKYKSRNVTDDVKKIARFVQDNCEKVGNRYGKSAQQNRLTLNMLVEIFLGSKSARIQTGMFGVGAAYSKHNAERLFKKLVLDNVLMEDLYITNSGQAVAYISAGPKAMSVLNGCMQVEFHETESASSIRKHRASVCEKVSKREEMVKKCLGELNDLCKKLGKVFGIHYYNIFSTATLKKIAETLSADPEVLLQIDGVTEDKLEKYGAEFIELLQKYSEWQLPAEAQAESSGWIDTTRGHQNDEDDNDDDDEGGGDVTSTYFRSNSGRGAKRKQGSYSRKPKRRKGSSGQNSSAKGGYSSNWSSSRGGGRGGGYRGGSRGAGRGSRSAPSGSAAKRPGFMSLPTPQAAARPFLKPSFSHL

Target ORF information:

RefSeq Version	XM_021467812.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467812.1

ORF Insert Sequence:

ATGTCGAGTC TTCCTCAAAA CAACCTGAAG GAGCATCTGG AGCGACACAA CAATGCTGCC 
CAGAACAAGC TGTCACTGCT CAAACCAAAG CCTGGGGGGT TTTGCTTCAA AAAGAAGTCT 
TCGTCAGGCA TCTCCAAAGT GGAAGTTCCT CAAAAGGTAA CTGGTTCAAG TGTCTTAGCA 
AACAGGAGTG TCAATATTCC CAGCAACTTT GAAGTAACCA AAAATCCTGT GACGTTTTCG 
CAAAAGCCAG AGAGAGTGGC ACCGAAAGTC AACTTCTTCA CTGCGCCCAG CAAACCGAAG 
ACAAATACAG TTAATCCACT AGCAAACCCA TTCGCTTTGA ACAAAACCCC TCTTGTCCAA 
TCTACCATTA AGGCTTTGCC CAAATCTGCA GCCTTGCCTA AGGACTCAAC TAATGATCAA 
ACTGTGGAGC TTAAGCATGA CATTTCTCAA CTCTCATTCA ATGAATGGGA TGACTTGGAT 
GATTTTGAAA CTCCAGTCAA GAGCAGAGTA GCATCCCCAG TTGCAGGGAC CTCTACGAAA 
AAGCCAAGTG TATCAGACCA GAACACATCC TCAAGCTGCT CTTCTAAGTG TGAGGAGACT 
AAAGTAAATG AGGAGTCACA AGCTACAGAA ACCATAACTG CCCCGAAGGA CGTCCTGTCA 
GCTGCTAATG GAGTCAGCAC AGAGACTGCA GAGAGAGAGC CAGAGGACTC ACCCATTAAA 
AAGTCCAAAA GACCTAAGAA ATCAGTCCAG CATACAGCAT TACTTAGTGA CACGGAAGAT 
GAGGAAATAA TCCACTGTGT TTCACCGGAT ACAAACCAGA AGAATTTCAA AAGTGTGGCA 
GTTGCTGAAA AAGATAAATG GGGTGAGCCA AATGTCATAG ACAGTGATGA CTGTGAGGAG 
AACAACCATT ATGAAGGTTT TGAGGATTTC ATCCCCCCGT CCCCTATTCC AGAAGAGATC 
AGTCTCTCTG TCTCAGATAA GGAGAAAAGT TCATCTGAGC CTGTTACTCC TGCTAACAAA 
AAAGAATCTT CTCGATCTGC CTCAGGCCTG CCAGCACCCT TGGATCAATC TGCCAAAGGA 
TTAAAAGGAG CTGATGACGC TCTCTTTAGT GTTATGGAAT CTATCTGTTG TCTAGTGGAC 
ACTATTCCTG AGCATGAGCT CATAGCGCTG ACCTGTGGGA CTGAGCTTTT ACTGCAGAGA 
GCACACAGGA AAAGGATTCT TGCTAAAGGT GGGTCATCTA GAACATCTCA TTCTGACTCA 
GTATCAACTC CTGGTTTCCT AAACAGACCT ACGTTTGGAG TTACACCCTC CAATCTGACA 
TCCCTGACCC CGGTAACTTC AGGAAAAAGA GAAGGTGTCA AAACTGGCTT TTCTTTTCGC 
AAATCCATTG CCTCTGTCAT GTCTGTGGGT GATGAGAGTG TTTTTGAGGA TTCTGACTGC 
ATCATAAATG GAGTCGAAAC ACCTGGTGGT ACCTGGAATC CTAACAGCTC CACTAAAATA 
TCAGCTGGTA GGGATACCTT TAACGGGTCA ATCCAAACTT TAAGCAAACC TGAGTCAAAG 
ACAGATAAAT GCTACTCAAG GTTGTCCTTC AATGAGTCAA GCAATCAGAC TGACCTGTTT 
TATTCCCCCA AGAGAGTGGA TTCAGGCAGC AGAAATGCTG ACAGCAGTGT TGAGATCAAC 
ATTGCAGGAT CCAGCTCTTT ACGAACAGGA GCAGAGCCAG TTGATGATTT TTTACTCGAT 
GACTTCGATA TTGATGATTT TGATGAGAAT GATATTCCAG ATTATTATGA AGAACCTCCA 
AGTGTCTTGG AATCAAGAAA TAACTCTGGT GTAAAAACAC CATCAGTACA GGAGGGAGGA 
TCATCAAAAT CTTTTGAGAG GAAGACATTC ACACCACCAG CACCAAAGTC TATTAAAACT 
CCCAACCCAG AACCTTTGTA CAGGAACCCA GCTCATGACC GCTTCAGAGG CTTTAACTTT 
CCTCATAGCC CAGAGATGAT GAAGATCTTC CATAAGAAGT TTGGACTTCA TCAGTTCCGC 
TTTAACCAGC TGGAAGCTAT TAATGCCTCG CTGTTAGGAG AAGACACATT TGTATTGATG 
CCTACAGGTG GTGGTAAAAG TCTCTGTTAT CAGCTTCCTG CTTGTGTCTC TGCTGGGGTG 
ACTGTAGTCA TTTCTCCTCT ACGCTCTTTA ATAGTTGACC AAGTACAAAA ACTCACCACG 
TTAGATATCT GTGCAACTAG TTTGTCCGGG GACAAAAAAG ATAGTGAGGC TGCCCGAATC 
TACATGCAGC TGTCCAGGAA AGATCCTGCC ATCAAACTGC TTTATGCTAC TCCTGAGAAG 
GTTTGTGCAA GTGGGCGGAT GATCAGTGCA CTTCAGAATC TGTATGAAAG AGGTCTGCTG 
GCTCGCTTGG TCATTGATGA GGCCCATTGC GTCAGTCAGT GGGGTCATGA TTTCAGACCA 
GACTACAAGC GCTTGCATGA GCTCAGGCGG ATGTTTCCCA ATGTTCCTAT AATGGCACTG 
ACGGCCACAG CCACTCCTAG AGTTCAGAAA GACATTCTTA ACCAATTGGC AATGACACGT 
CCTCAGGTAT TTACTATGAG CTTTAACAGG AACAATCTGA AATACTCAGT TTTGCCTAAG 
AAGCCCAAGA AGGTTGATGA GGAATGCATT CAGTGGATTA AGAAATATTA CCCACGTGAC 
TCTGGGATTG TGTACTGCTT GTCTCGTAAT GACTGCGACA CTCTGGCTGA CAGTCTTCAG 
AGGGCAGGGA TCGCAGCGCT GGCGTATCAT GCTGGCCTAA GTGACAGCGA CAGGGAATAT 
GTCCAGAACA AGTGGATCAA CCAGGACGGC TGTCAGGTTA TGTGCGCAAC CATAGCATTT 
GGCATGGGAA TCGATAAGCC TGATGTCCGT TATGTGATCC ATGCGAGTCT CCCCAAATCA 
GTGGAGGGTT ACTACCAGGA GTCAGGCAGA GCAGGGCGAG ATGGAGAGAT TTCCCATTGT 
GTCCTCTTCT ATTCCTACAG TGACGTTATC CGCATCAAGA GACTCATTGC TATGGATAAA 
GATGGCAACC AGCAATCCAA AGCCACCCAC ATCAATAACC TGCACAGCAT GGTGCACTTC 
TGTGAGAATG TGGCTGAATG CAGAAGGATC CAGCTGCTGG CTTACTTTGG GGAGCACACG 
TTTAACACGA GCTTCTGTAA AGAGCATCCT GAGGTCATCT GTGACAACTG TGCCAGACCA 
AACAAATATA AATCCAGAAA TGTGACCGAT GACGTTAAGA AGATTGCGAG GTTTGTGCAG 
GACAACTGTG AGAAGGTTGG AAACAGATAC GGCAAATCCG CCCAGCAGAA CAGGCTCACT 
CTCAACATGC TGGTTGAAAT ATTTCTGGGC TCCAAAAGCG CTCGCATCCA GACTGGGATG 
TTCGGAGTTG GAGCAGCTTA TTCCAAACAC AACGCTGAGA GGCTCTTTAA GAAGCTGGTG 
CTGGATAATG TTCTGATGGA GGATCTGTAC ATCACTAACA GTGGCCAGGC TGTGGCCTAT 
ATCTCTGCAG GCCCTAAAGC TATGAGTGTA CTGAATGGTT GCATGCAGGT TGAATTTCAT 
GAGACCGAGA GCGCATCCAG CATCAGAAAG CACAGAGCGT CTGTGTGTGA GAAGGTGTCT 
AAGAGAGAGG AGATGGTGAA AAAGTGTCTG GGTGAACTTA ATGATCTATG CAAGAAGTTG 
GGCAAAGTCT TCGGCATTCA TTACTACAAT ATCTTCTCCA CCGCTACACT CAAAAAGATT 
GCTGAGACTC TCTCGGCTGA TCCGGAGGTT CTGCTGCAGA TTGATGGTGT AACTGAAGAC 
AAACTGGAGA AATATGGAGC TGAGTTTATT GAACTGCTAC AGAAATACTC TGAGTGGCAG 
CTACCTGCTG AAGCACAGGC TGAGAGTTCA GGCTGGATCG ACACGACCAG AGGCCATCAA 
AATGATGAAG ACGACAACGA TGATGATGAT GAAGGAGGTG GGGATGTCAC ATCGACATAT 
TTCAGAAGCA ACTCTGGACG AGGAGCCAAG AGAAAGCAAG GCTCTTACTC CAGAAAACCC 
AAACGAAGAA AAGGCTCCAG TGGCCAGAAC TCCTCTGCAA AAGGTGGATA TAGCAGTAAC 
TGGTCTTCAT CACGAGGAGG AGGAAGAGGA GGTGGATACA GGGGTGGCAG TCGAGGAGCA 
GGCCGAGGCT CCAGATCTGC TCCTTCAGGC TCAGCAGCAA AAAGGCCGGG CTTCATGTCG 
TTACCAACAC CGCAGGCTGC TGCTCGTCCA TTCCTCAAAC CGTCCTTCTC ACACCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( XM_017352050.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207539.1 Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X2, mRNA.

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CloneID	ODa55903
Clone ID Related Accession (Same CDS sequence)	XM_021467812.1 , XM_017352050.1 , XM_017352050.2
Accession Version	XM_017352050.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Bloom syndrome protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGTC TTCCTCAAAA CAACCTGAAG GAGCATCTGG AGCGACACAA CAATGCTGCC 
CAGAACAAGC TGTCACTGCT CAAACCAAAG CCTGGGGGGT TTTGCTTCAA AAAGAAGTCT 
TCGTCAGGCA TCTCCAAAGT GGAAGTTCCT CAAAAGGTAA CTGGTTCAAG TGTCTTAGCA 
AACAGGAGTG TCAATATTCC CAGCAACTTT GAAGTAACCA AAAATCCTGT GACGTTTTCG 
CAAAAGCCAG AGAGAGTGGC ACCGAAAGTC AACTTCTTCA CTGCGCCCAG CAAACCGAAG 
ACAAATACAG TTAATCCACT AGCAAACCCA TTCGCTTTGA ACAAAACCCC TCTTGTCCAA 
TCTACCATTA AGGCTTTGCC CAAATCTGCA GCCTTGCCTA AGGACTCAAC TAATGATCAA 
ACTGTGGAGC TTAAGCATGA CATTTCTCAA CTCTCATTCA ATGAATGGGA TGACTTGGAT 
GATTTTGAAA CTCCAGTCAA GAGCAGAGTA GCATCCCCAG TTGCAGGGAC CTCTACGAAA 
AAGCCAAGTG TATCAGACCA GAACACATCC TCAAGCTGCT CTTCTAAGTG TGAGGAGACT 
AAAGTAAATG AGGAGTCACA AGCTACAGAA ACCATAACTG CCCCGAAGGA CGTCCTGTCA 
GCTGCTAATG GAGTCAGCAC AGAGACTGCA GAGAGAGAGC CAGAGGACTC ACCCATTAAA 
AAGTCCAAAA GACCTAAGAA ATCAGTCCAG CATACAGCAT TACTTAGTGA CACGGAAGAT 
GAGGAAATAA TCCACTGTGT TTCACCGGAT ACAAACCAGA AGAATTTCAA AAGTGTGGCA 
GTTGCTGAAA AAGATAAATG GGGTGAGCCA AATGTCATAG ACAGTGATGA CTGTGAGGAG 
AACAACCATT ATGAAGGTTT TGAGGATTTC ATCCCCCCGT CCCCTATTCC AGAAGAGATC 
AGTCTCTCTG TCTCAGATAA GGAGAAAAGT TCATCTGAGC CTGTTACTCC TGCTAACAAA 
AAAGAATCTT CTCGATCTGC CTCAGGCCTG CCAGCACCCT TGGATCAATC TGCCAAAGGA 
TTAAAAGGAG CTGATGACGC TCTCTTTAGT GTTATGGAAT CTATCTGTTG TCTAGTGGAC 
ACTATTCCTG AGCATGAGCT CATAGCGCTG ACCTGTGGGA CTGAGCTTTT ACTGCAGAGA 
GCACACAGGA AAAGGATTCT TGCTAAAGGT GGGTCATCTA GAACATCTCA TTCTGACTCA 
GTATCAACTC CTGGTTTCCT AAACAGACCT ACGTTTGGAG TTACACCCTC CAATCTGACA 
TCCCTGACCC CGGTAACTTC AGGAAAAAGA GAAGGTGTCA AAACTGGCTT TTCTTTTCGC 
AAATCCATTG CCTCTGTCAT GTCTGTGGGT GATGAGAGTG TTTTTGAGGA TTCTGACTGC 
ATCATAAATG GAGTCGAAAC ACCTGGTGGT ACCTGGAATC CTAACAGCTC CACTAAAATA 
TCAGCTGGTA GGGATACCTT TAACGGGTCA ATCCAAACTT TAAGCAAACC TGAGTCAAAG 
ACAGATAAAT GCTACTCAAG GTTGTCCTTC AATGAGTCAA GCAATCAGAC TGACCTGTTT 
TATTCCCCCA AGAGAGTGGA TTCAGGCAGC AGAAATGCTG ACAGCAGTGT TGAGATCAAC 
ATTGCAGGAT CCAGCTCTTT ACGAACAGGA GCAGAGCCAG TTGATGATTT TTTACTCGAT 
GACTTCGATA TTGATGATTT TGATGAGAAT GATATTCCAG ATTATTATGA AGAACCTCCA 
AGTGTCTTGG AATCAAGAAA TAACTCTGGT GTAAAAACAC CATCAGTACA GGAGGGAGGA 
TCATCAAAAT CTTTTGAGAG GAAGACATTC ACACCACCAG CACCAAAGTC TATTAAAACT 
CCCAACCCAG AACCTTTGTA CAGGAACCCA GCTCATGACC GCTTCAGAGG CTTTAACTTT 
CCTCATAGCC CAGAGATGAT GAAGATCTTC CATAAGAAGT TTGGACTTCA TCAGTTCCGC 
TTTAACCAGC TGGAAGCTAT TAATGCCTCG CTGTTAGGAG AAGACACATT TGTATTGATG 
CCTACAGGTG GTGGTAAAAG TCTCTGTTAT CAGCTTCCTG CTTGTGTCTC TGCTGGGGTG 
ACTGTAGTCA TTTCTCCTCT ACGCTCTTTA ATAGTTGACC AAGTACAAAA ACTCACCACG 
TTAGATATCT GTGCAACTAG TTTGTCCGGG GACAAAAAAG ATAGTGAGGC TGCCCGAATC 
TACATGCAGC TGTCCAGGAA AGATCCTGCC ATCAAACTGC TTTATGCTAC TCCTGAGAAG 
GTTTGTGCAA GTGGGCGGAT GATCAGTGCA CTTCAGAATC TGTATGAAAG AGGTCTGCTG 
GCTCGCTTGG TCATTGATGA GGCCCATTGC GTCAGTCAGT GGGGTCATGA TTTCAGACCA 
GACTACAAGC GCTTGCATGA GCTCAGGCGG ATGTTTCCCA ATGTTCCTAT AATGGCACTG 
ACGGCCACAG CCACTCCTAG AGTTCAGAAA GACATTCTTA ACCAATTGGC AATGACACGT 
CCTCAGGTAT TTACTATGAG CTTTAACAGG AACAATCTGA AATACTCAGT TTTGCCTAAG 
AAGCCCAAGA AGGTTGATGA GGAATGCATT CAGTGGATTA AGAAATATTA CCCACGTGAC 
TCTGGGATTG TGTACTGCTT GTCTCGTAAT GACTGCGACA CTCTGGCTGA CAGTCTTCAG 
AGGGCAGGGA TCGCAGCGCT GGCGTATCAT GCTGGCCTAA GTGACAGCGA CAGGGAATAT 
GTCCAGAACA AGTGGATCAA CCAGGACGGC TGTCAGGTTA TGTGCGCAAC CATAGCATTT 
GGCATGGGAA TCGATAAGCC TGATGTCCGT TATGTGATCC ATGCGAGTCT CCCCAAATCA 
GTGGAGGGTT ACTACCAGGA GTCAGGCAGA GCAGGGCGAG ATGGAGAGAT TTCCCATTGT 
GTCCTCTTCT ATTCCTACAG TGACGTTATC CGCATCAAGA GACTCATTGC TATGGATAAA 
GATGGCAACC AGCAATCCAA AGCCACCCAC ATCAATAACC TGCACAGCAT GGTGCACTTC 
TGTGAGAATG TGGCTGAATG CAGAAGGATC CAGCTGCTGG CTTACTTTGG GGAGCACACG 
TTTAACACGA GCTTCTGTAA AGAGCATCCT GAGGTCATCT GTGACAACTG TGCCAGACCA 
AACAAATATA AATCCAGAAA TGTGACCGAT GACGTTAAGA AGATTGCGAG GTTTGTGCAG 
GACAACTGTG AGAAGGTTGG AAACAGATAC GGCAAATCCG CCCAGCAGAA CAGGCTCACT 
CTCAACATGC TGGTTGAAAT ATTTCTGGGC TCCAAAAGCG CTCGCATCCA GACTGGGATG 
TTCGGAGTTG GAGCAGCTTA TTCCAAACAC AACGCTGAGA GGCTCTTTAA GAAGCTGGTG 
CTGGATAATG TTCTGATGGA GGATCTGTAC ATCACTAACA GTGGCCAGGC TGTGGCCTAT 
ATCTCTGCAG GCCCTAAAGC TATGAGTGTA CTGAATGGTT GCATGCAGGT TGAATTTCAT 
GAGACCGAGA GCGCATCCAG CATCAGAAAG CACAGAGCGT CTGTGTGTGA GAAGGTGTCT 
AAGAGAGAGG AGATGGTGAA AAAGTGTCTG GGTGAACTTA ATGATCTATG CAAGAAGTTG 
GGCAAAGTCT TCGGCATTCA TTACTACAAT ATCTTCTCCA CCGCTACACT CAAAAAGATT 
GCTGAGACTC TCTCGGCTGA TCCGGAGGTT CTGCTGCAGA TTGATGGTGT AACTGAAGAC 
AAACTGGAGA AATATGGAGC TGAGTTTATT GAACTGCTAC AGAAATACTC TGAGTGGCAG 
CTACCTGCTG AAGCACAGGC TGAGAGTTCA GGCTGGATCG ACACGACCAG AGGCCATCAA 
AATGATGAAG ACGACAACGA TGATGATGAT GAAGGAGGTG GGGATGTCAC ATCGACATAT 
TTCAGAAGCA ACTCTGGACG AGGAGCCAAG AGAAAGCAAG GCTCTTACTC CAGAAAACCC 
AAACGAAGAA AAGGCTCCAG TGGCCAGAAC TCCTCTGCAA AAGGTGGATA TAGCAGTAAC 
TGGTCTTCAT CACGAGGAGG AGGAAGAGGA GGTGGATACA GGGGTGGCAG TCGAGGAGCA 
GGCCGAGGCT CCAGATCTGC TCCTTCAGGC TCAGCAGCAA AAAGGCCGGG CTTCATGTCG 
TTACCAACAC CGCAGGCTGC TGCTCGTCCA TTCCTCAAAC CGTCCTTCTC ACACCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207539.1
CDS	539..4858
Translation	MSSLPQNNLKEHLERHNNAAQNKLSLLKPKPGGFCFKKKSSSGISKVEVPQKVTGSSVLANRSVNIPSNFEVTKNPVTFSQKPERVAPKVNFFTAPSKPKTNTVNPLANPFALNKTPLVQSTIKALPKSAALPKDSTNDQTVELKHDISQLSFNEWDDLDDFETPVKSRVASPVAGTSTKKPSVSDQNTSSSCSSKCEETKVNEESQATETITAPKDVLSAANGVSTETAEREPEDSPIKKSKRPKKSVQHTALLSDTEDEEIIHCVSPDTNQKNFKSVAVAEKDKWGEPNVIDSDDCEENNHYEGFEDFIPPSPIPEEISLSVSDKEKSSSEPVTPANKKESSRSASGLPAPLDQSAKGLKGADDALFSVMESICCLVDTIPEHELIALTCGTELLLQRAHRKRILAKGGSSRTSHSDSVSTPGFLNRPTFGVTPSNLTSLTPVTSGKREGVKTGFSFRKSIASVMSVGDESVFEDSDCIINGVETPGGTWNPNSSTKISAGRDTFNGSIQTLSKPESKTDKCYSRLSFNESSNQTDLFYSPKRVDSGSRNADSSVEINIAGSSSLRTGAEPVDDFLLDDFDIDDFDENDIPDYYEEPPSVLESRNNSGVKTPSVQEGGSSKSFERKTFTPPAPKSIKTPNPEPLYRNPAHDRFRGFNFPHSPEMMKIFHKKFGLHQFRFNQLEAINASLLGEDTFVLMPTGGGKSLCYQLPACVSAGVTVVISPLRSLIVDQVQKLTTLDICATSLSGDKKDSEAARIYMQLSRKDPAIKLLYATPEKVCASGRMISALQNLYERGLLARLVIDEAHCVSQWGHDFRPDYKRLHELRRMFPNVPIMALTATATPRVQKDILNQLAMTRPQVFTMSFNRNNLKYSVLPKKPKKVDEECIQWIKKYYPRDSGIVYCLSRNDCDTLADSLQRAGIAALAYHAGLSDSDREYVQNKWINQDGCQVMCATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCVLFYSYSDVIRIKRLIAMDKDGNQQSKATHINNLHSMVHFCENVAECRRIQLLAYFGEHTFNTSFCKEHPEVICDNCARPNKYKSRNVTDDVKKIARFVQDNCEKVGNRYGKSAQQNRLTLNMLVEIFLGSKSARIQTGMFGVGAAYSKHNAERLFKKLVLDNVLMEDLYITNSGQAVAYISAGPKAMSVLNGCMQVEFHETESASSIRKHRASVCEKVSKREEMVKKCLGELNDLCKKLGKVFGIHYYNIFSTATLKKIAETLSADPEVLLQIDGVTEDKLEKYGAEFIELLQKYSEWQLPAEAQAESSGWIDTTRGHQNDEDDNDDDDEGGGDVTSTYFRSNSGRGAKRKQGSYSRKPKRRKGSSGQNSSAKGGYSSNWSSSRGGGRGGGYRGGSRGAGRGSRSAPSGSAAKRPGFMSLPTPQAAARPFLKPSFSHL

Target ORF information:

RefSeq Version	XM_017352050.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352050.1

ORF Insert Sequence:

ATGTCGAGTC TTCCTCAAAA CAACCTGAAG GAGCATCTGG AGCGACACAA CAATGCTGCC 
CAGAACAAGC TGTCACTGCT CAAACCAAAG CCTGGGGGGT TTTGCTTCAA AAAGAAGTCT 
TCGTCAGGCA TCTCCAAAGT GGAAGTTCCT CAAAAGGTAA CTGGTTCAAG TGTCTTAGCA 
AACAGGAGTG TCAATATTCC CAGCAACTTT GAAGTAACCA AAAATCCTGT GACGTTTTCG 
CAAAAGCCAG AGAGAGTGGC ACCGAAAGTC AACTTCTTCA CTGCGCCCAG CAAACCGAAG 
ACAAATACAG TTAATCCACT AGCAAACCCA TTCGCTTTGA ACAAAACCCC TCTTGTCCAA 
TCTACCATTA AGGCTTTGCC CAAATCTGCA GCCTTGCCTA AGGACTCAAC TAATGATCAA 
ACTGTGGAGC TTAAGCATGA CATTTCTCAA CTCTCATTCA ATGAATGGGA TGACTTGGAT 
GATTTTGAAA CTCCAGTCAA GAGCAGAGTA GCATCCCCAG TTGCAGGGAC CTCTACGAAA 
AAGCCAAGTG TATCAGACCA GAACACATCC TCAAGCTGCT CTTCTAAGTG TGAGGAGACT 
AAAGTAAATG AGGAGTCACA AGCTACAGAA ACCATAACTG CCCCGAAGGA CGTCCTGTCA 
GCTGCTAATG GAGTCAGCAC AGAGACTGCA GAGAGAGAGC CAGAGGACTC ACCCATTAAA 
AAGTCCAAAA GACCTAAGAA ATCAGTCCAG CATACAGCAT TACTTAGTGA CACGGAAGAT 
GAGGAAATAA TCCACTGTGT TTCACCGGAT ACAAACCAGA AGAATTTCAA AAGTGTGGCA 
GTTGCTGAAA AAGATAAATG GGGTGAGCCA AATGTCATAG ACAGTGATGA CTGTGAGGAG 
AACAACCATT ATGAAGGTTT TGAGGATTTC ATCCCCCCGT CCCCTATTCC AGAAGAGATC 
AGTCTCTCTG TCTCAGATAA GGAGAAAAGT TCATCTGAGC CTGTTACTCC TGCTAACAAA 
AAAGAATCTT CTCGATCTGC CTCAGGCCTG CCAGCACCCT TGGATCAATC TGCCAAAGGA 
TTAAAAGGAG CTGATGACGC TCTCTTTAGT GTTATGGAAT CTATCTGTTG TCTAGTGGAC 
ACTATTCCTG AGCATGAGCT CATAGCGCTG ACCTGTGGGA CTGAGCTTTT ACTGCAGAGA 
GCACACAGGA AAAGGATTCT TGCTAAAGGT GGGTCATCTA GAACATCTCA TTCTGACTCA 
GTATCAACTC CTGGTTTCCT AAACAGACCT ACGTTTGGAG TTACACCCTC CAATCTGACA 
TCCCTGACCC CGGTAACTTC AGGAAAAAGA GAAGGTGTCA AAACTGGCTT TTCTTTTCGC 
AAATCCATTG CCTCTGTCAT GTCTGTGGGT GATGAGAGTG TTTTTGAGGA TTCTGACTGC 
ATCATAAATG GAGTCGAAAC ACCTGGTGGT ACCTGGAATC CTAACAGCTC CACTAAAATA 
TCAGCTGGTA GGGATACCTT TAACGGGTCA ATCCAAACTT TAAGCAAACC TGAGTCAAAG 
ACAGATAAAT GCTACTCAAG GTTGTCCTTC AATGAGTCAA GCAATCAGAC TGACCTGTTT 
TATTCCCCCA AGAGAGTGGA TTCAGGCAGC AGAAATGCTG ACAGCAGTGT TGAGATCAAC 
ATTGCAGGAT CCAGCTCTTT ACGAACAGGA GCAGAGCCAG TTGATGATTT TTTACTCGAT 
GACTTCGATA TTGATGATTT TGATGAGAAT GATATTCCAG ATTATTATGA AGAACCTCCA 
AGTGTCTTGG AATCAAGAAA TAACTCTGGT GTAAAAACAC CATCAGTACA GGAGGGAGGA 
TCATCAAAAT CTTTTGAGAG GAAGACATTC ACACCACCAG CACCAAAGTC TATTAAAACT 
CCCAACCCAG AACCTTTGTA CAGGAACCCA GCTCATGACC GCTTCAGAGG CTTTAACTTT 
CCTCATAGCC CAGAGATGAT GAAGATCTTC CATAAGAAGT TTGGACTTCA TCAGTTCCGC 
TTTAACCAGC TGGAAGCTAT TAATGCCTCG CTGTTAGGAG AAGACACATT TGTATTGATG 
CCTACAGGTG GTGGTAAAAG TCTCTGTTAT CAGCTTCCTG CTTGTGTCTC TGCTGGGGTG 
ACTGTAGTCA TTTCTCCTCT ACGCTCTTTA ATAGTTGACC AAGTACAAAA ACTCACCACG 
TTAGATATCT GTGCAACTAG TTTGTCCGGG GACAAAAAAG ATAGTGAGGC TGCCCGAATC 
TACATGCAGC TGTCCAGGAA AGATCCTGCC ATCAAACTGC TTTATGCTAC TCCTGAGAAG 
GTTTGTGCAA GTGGGCGGAT GATCAGTGCA CTTCAGAATC TGTATGAAAG AGGTCTGCTG 
GCTCGCTTGG TCATTGATGA GGCCCATTGC GTCAGTCAGT GGGGTCATGA TTTCAGACCA 
GACTACAAGC GCTTGCATGA GCTCAGGCGG ATGTTTCCCA ATGTTCCTAT AATGGCACTG 
ACGGCCACAG CCACTCCTAG AGTTCAGAAA GACATTCTTA ACCAATTGGC AATGACACGT 
CCTCAGGTAT TTACTATGAG CTTTAACAGG AACAATCTGA AATACTCAGT TTTGCCTAAG 
AAGCCCAAGA AGGTTGATGA GGAATGCATT CAGTGGATTA AGAAATATTA CCCACGTGAC 
TCTGGGATTG TGTACTGCTT GTCTCGTAAT GACTGCGACA CTCTGGCTGA CAGTCTTCAG 
AGGGCAGGGA TCGCAGCGCT GGCGTATCAT GCTGGCCTAA GTGACAGCGA CAGGGAATAT 
GTCCAGAACA AGTGGATCAA CCAGGACGGC TGTCAGGTTA TGTGCGCAAC CATAGCATTT 
GGCATGGGAA TCGATAAGCC TGATGTCCGT TATGTGATCC ATGCGAGTCT CCCCAAATCA 
GTGGAGGGTT ACTACCAGGA GTCAGGCAGA GCAGGGCGAG ATGGAGAGAT TTCCCATTGT 
GTCCTCTTCT ATTCCTACAG TGACGTTATC CGCATCAAGA GACTCATTGC TATGGATAAA 
GATGGCAACC AGCAATCCAA AGCCACCCAC ATCAATAACC TGCACAGCAT GGTGCACTTC 
TGTGAGAATG TGGCTGAATG CAGAAGGATC CAGCTGCTGG CTTACTTTGG GGAGCACACG 
TTTAACACGA GCTTCTGTAA AGAGCATCCT GAGGTCATCT GTGACAACTG TGCCAGACCA 
AACAAATATA AATCCAGAAA TGTGACCGAT GACGTTAAGA AGATTGCGAG GTTTGTGCAG 
GACAACTGTG AGAAGGTTGG AAACAGATAC GGCAAATCCG CCCAGCAGAA CAGGCTCACT 
CTCAACATGC TGGTTGAAAT ATTTCTGGGC TCCAAAAGCG CTCGCATCCA GACTGGGATG 
TTCGGAGTTG GAGCAGCTTA TTCCAAACAC AACGCTGAGA GGCTCTTTAA GAAGCTGGTG 
CTGGATAATG TTCTGATGGA GGATCTGTAC ATCACTAACA GTGGCCAGGC TGTGGCCTAT 
ATCTCTGCAG GCCCTAAAGC TATGAGTGTA CTGAATGGTT GCATGCAGGT TGAATTTCAT 
GAGACCGAGA GCGCATCCAG CATCAGAAAG CACAGAGCGT CTGTGTGTGA GAAGGTGTCT 
AAGAGAGAGG AGATGGTGAA AAAGTGTCTG GGTGAACTTA ATGATCTATG CAAGAAGTTG 
GGCAAAGTCT TCGGCATTCA TTACTACAAT ATCTTCTCCA CCGCTACACT CAAAAAGATT 
GCTGAGACTC TCTCGGCTGA TCCGGAGGTT CTGCTGCAGA TTGATGGTGT AACTGAAGAC 
AAACTGGAGA AATATGGAGC TGAGTTTATT GAACTGCTAC AGAAATACTC TGAGTGGCAG 
CTACCTGCTG AAGCACAGGC TGAGAGTTCA GGCTGGATCG ACACGACCAG AGGCCATCAA 
AATGATGAAG ACGACAACGA TGATGATGAT GAAGGAGGTG GGGATGTCAC ATCGACATAT 
TTCAGAAGCA ACTCTGGACG AGGAGCCAAG AGAAAGCAAG GCTCTTACTC CAGAAAACCC 
AAACGAAGAA AAGGCTCCAG TGGCCAGAAC TCCTCTGCAA AAGGTGGATA TAGCAGTAAC 
TGGTCTTCAT CACGAGGAGG AGGAAGAGGA GGTGGATACA GGGGTGGCAG TCGAGGAGCA 
GGCCGAGGCT CCAGATCTGC TCCTTCAGGC TCAGCAGCAA AAAGGCCGGG CTTCATGTCG 
TTACCAACAC CGCAGGCTGC TGCTCGTCCA TTCCTCAAAC CGTCCTTCTC ACACCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( XM_017352050.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207539.1 Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X3, mRNA.

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CloneID	ODa55903
Clone ID Related Accession (Same CDS sequence)	XM_021467812.1 , XM_017352050.1 , XM_017352050.2
Accession Version	XM_017352050.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Bloom syndrome protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGTC TTCCTCAAAA CAACCTGAAG GAGCATCTGG AGCGACACAA CAATGCTGCC 
CAGAACAAGC TGTCACTGCT CAAACCAAAG CCTGGGGGGT TTTGCTTCAA AAAGAAGTCT 
TCGTCAGGCA TCTCCAAAGT GGAAGTTCCT CAAAAGGTAA CTGGTTCAAG TGTCTTAGCA 
AACAGGAGTG TCAATATTCC CAGCAACTTT GAAGTAACCA AAAATCCTGT GACGTTTTCG 
CAAAAGCCAG AGAGAGTGGC ACCGAAAGTC AACTTCTTCA CTGCGCCCAG CAAACCGAAG 
ACAAATACAG TTAATCCACT AGCAAACCCA TTCGCTTTGA ACAAAACCCC TCTTGTCCAA 
TCTACCATTA AGGCTTTGCC CAAATCTGCA GCCTTGCCTA AGGACTCAAC TAATGATCAA 
ACTGTGGAGC TTAAGCATGA CATTTCTCAA CTCTCATTCA ATGAATGGGA TGACTTGGAT 
GATTTTGAAA CTCCAGTCAA GAGCAGAGTA GCATCCCCAG TTGCAGGGAC CTCTACGAAA 
AAGCCAAGTG TATCAGACCA GAACACATCC TCAAGCTGCT CTTCTAAGTG TGAGGAGACT 
AAAGTAAATG AGGAGTCACA AGCTACAGAA ACCATAACTG CCCCGAAGGA CGTCCTGTCA 
GCTGCTAATG GAGTCAGCAC AGAGACTGCA GAGAGAGAGC CAGAGGACTC ACCCATTAAA 
AAGTCCAAAA GACCTAAGAA ATCAGTCCAG CATACAGCAT TACTTAGTGA CACGGAAGAT 
GAGGAAATAA TCCACTGTGT TTCACCGGAT ACAAACCAGA AGAATTTCAA AAGTGTGGCA 
GTTGCTGAAA AAGATAAATG GGGTGAGCCA AATGTCATAG ACAGTGATGA CTGTGAGGAG 
AACAACCATT ATGAAGGTTT TGAGGATTTC ATCCCCCCGT CCCCTATTCC AGAAGAGATC 
AGTCTCTCTG TCTCAGATAA GGAGAAAAGT TCATCTGAGC CTGTTACTCC TGCTAACAAA 
AAAGAATCTT CTCGATCTGC CTCAGGCCTG CCAGCACCCT TGGATCAATC TGCCAAAGGA 
TTAAAAGGAG CTGATGACGC TCTCTTTAGT GTTATGGAAT CTATCTGTTG TCTAGTGGAC 
ACTATTCCTG AGCATGAGCT CATAGCGCTG ACCTGTGGGA CTGAGCTTTT ACTGCAGAGA 
GCACACAGGA AAAGGATTCT TGCTAAAGGT GGGTCATCTA GAACATCTCA TTCTGACTCA 
GTATCAACTC CTGGTTTCCT AAACAGACCT ACGTTTGGAG TTACACCCTC CAATCTGACA 
TCCCTGACCC CGGTAACTTC AGGAAAAAGA GAAGGTGTCA AAACTGGCTT TTCTTTTCGC 
AAATCCATTG CCTCTGTCAT GTCTGTGGGT GATGAGAGTG TTTTTGAGGA TTCTGACTGC 
ATCATAAATG GAGTCGAAAC ACCTGGTGGT ACCTGGAATC CTAACAGCTC CACTAAAATA 
TCAGCTGGTA GGGATACCTT TAACGGGTCA ATCCAAACTT TAAGCAAACC TGAGTCAAAG 
ACAGATAAAT GCTACTCAAG GTTGTCCTTC AATGAGTCAA GCAATCAGAC TGACCTGTTT 
TATTCCCCCA AGAGAGTGGA TTCAGGCAGC AGAAATGCTG ACAGCAGTGT TGAGATCAAC 
ATTGCAGGAT CCAGCTCTTT ACGAACAGGA GCAGAGCCAG TTGATGATTT TTTACTCGAT 
GACTTCGATA TTGATGATTT TGATGAGAAT GATATTCCAG ATTATTATGA AGAACCTCCA 
AGTGTCTTGG AATCAAGAAA TAACTCTGGT GTAAAAACAC CATCAGTACA GGAGGGAGGA 
TCATCAAAAT CTTTTGAGAG GAAGACATTC ACACCACCAG CACCAAAGTC TATTAAAACT 
CCCAACCCAG AACCTTTGTA CAGGAACCCA GCTCATGACC GCTTCAGAGG CTTTAACTTT 
CCTCATAGCC CAGAGATGAT GAAGATCTTC CATAAGAAGT TTGGACTTCA TCAGTTCCGC 
TTTAACCAGC TGGAAGCTAT TAATGCCTCG CTGTTAGGAG AAGACACATT TGTATTGATG 
CCTACAGGTG GTGGTAAAAG TCTCTGTTAT CAGCTTCCTG CTTGTGTCTC TGCTGGGGTG 
ACTGTAGTCA TTTCTCCTCT ACGCTCTTTA ATAGTTGACC AAGTACAAAA ACTCACCACG 
TTAGATATCT GTGCAACTAG TTTGTCCGGG GACAAAAAAG ATAGTGAGGC TGCCCGAATC 
TACATGCAGC TGTCCAGGAA AGATCCTGCC ATCAAACTGC TTTATGCTAC TCCTGAGAAG 
GTTTGTGCAA GTGGGCGGAT GATCAGTGCA CTTCAGAATC TGTATGAAAG AGGTCTGCTG 
GCTCGCTTGG TCATTGATGA GGCCCATTGC GTCAGTCAGT GGGGTCATGA TTTCAGACCA 
GACTACAAGC GCTTGCATGA GCTCAGGCGG ATGTTTCCCA ATGTTCCTAT AATGGCACTG 
ACGGCCACAG CCACTCCTAG AGTTCAGAAA GACATTCTTA ACCAATTGGC AATGACACGT 
CCTCAGGTAT TTACTATGAG CTTTAACAGG AACAATCTGA AATACTCAGT TTTGCCTAAG 
AAGCCCAAGA AGGTTGATGA GGAATGCATT CAGTGGATTA AGAAATATTA CCCACGTGAC 
TCTGGGATTG TGTACTGCTT GTCTCGTAAT GACTGCGACA CTCTGGCTGA CAGTCTTCAG 
AGGGCAGGGA TCGCAGCGCT GGCGTATCAT GCTGGCCTAA GTGACAGCGA CAGGGAATAT 
GTCCAGAACA AGTGGATCAA CCAGGACGGC TGTCAGGTTA TGTGCGCAAC CATAGCATTT 
GGCATGGGAA TCGATAAGCC TGATGTCCGT TATGTGATCC ATGCGAGTCT CCCCAAATCA 
GTGGAGGGTT ACTACCAGGA GTCAGGCAGA GCAGGGCGAG ATGGAGAGAT TTCCCATTGT 
GTCCTCTTCT ATTCCTACAG TGACGTTATC CGCATCAAGA GACTCATTGC TATGGATAAA 
GATGGCAACC AGCAATCCAA AGCCACCCAC ATCAATAACC TGCACAGCAT GGTGCACTTC 
TGTGAGAATG TGGCTGAATG CAGAAGGATC CAGCTGCTGG CTTACTTTGG GGAGCACACG 
TTTAACACGA GCTTCTGTAA AGAGCATCCT GAGGTCATCT GTGACAACTG TGCCAGACCA 
AACAAATATA AATCCAGAAA TGTGACCGAT GACGTTAAGA AGATTGCGAG GTTTGTGCAG 
GACAACTGTG AGAAGGTTGG AAACAGATAC GGCAAATCCG CCCAGCAGAA CAGGCTCACT 
CTCAACATGC TGGTTGAAAT ATTTCTGGGC TCCAAAAGCG CTCGCATCCA GACTGGGATG 
TTCGGAGTTG GAGCAGCTTA TTCCAAACAC AACGCTGAGA GGCTCTTTAA GAAGCTGGTG 
CTGGATAATG TTCTGATGGA GGATCTGTAC ATCACTAACA GTGGCCAGGC TGTGGCCTAT 
ATCTCTGCAG GCCCTAAAGC TATGAGTGTA CTGAATGGTT GCATGCAGGT TGAATTTCAT 
GAGACCGAGA GCGCATCCAG CATCAGAAAG CACAGAGCGT CTGTGTGTGA GAAGGTGTCT 
AAGAGAGAGG AGATGGTGAA AAAGTGTCTG GGTGAACTTA ATGATCTATG CAAGAAGTTG 
GGCAAAGTCT TCGGCATTCA TTACTACAAT ATCTTCTCCA CCGCTACACT CAAAAAGATT 
GCTGAGACTC TCTCGGCTGA TCCGGAGGTT CTGCTGCAGA TTGATGGTGT AACTGAAGAC 
AAACTGGAGA AATATGGAGC TGAGTTTATT GAACTGCTAC AGAAATACTC TGAGTGGCAG 
CTACCTGCTG AAGCACAGGC TGAGAGTTCA GGCTGGATCG ACACGACCAG AGGCCATCAA 
AATGATGAAG ACGACAACGA TGATGATGAT GAAGGAGGTG GGGATGTCAC ATCGACATAT 
TTCAGAAGCA ACTCTGGACG AGGAGCCAAG AGAAAGCAAG GCTCTTACTC CAGAAAACCC 
AAACGAAGAA AAGGCTCCAG TGGCCAGAAC TCCTCTGCAA AAGGTGGATA TAGCAGTAAC 
TGGTCTTCAT CACGAGGAGG AGGAAGAGGA GGTGGATACA GGGGTGGCAG TCGAGGAGCA 
GGCCGAGGCT CCAGATCTGC TCCTTCAGGC TCAGCAGCAA AAAGGCCGGG CTTCATGTCG 
TTACCAACAC CGCAGGCTGC TGCTCGTCCA TTCCTCAAAC CGTCCTTCTC ACACCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207539.1
CDS	394..4713
Translation	MSSLPQNNLKEHLERHNNAAQNKLSLLKPKPGGFCFKKKSSSGISKVEVPQKVTGSSVLANRSVNIPSNFEVTKNPVTFSQKPERVAPKVNFFTAPSKPKTNTVNPLANPFALNKTPLVQSTIKALPKSAALPKDSTNDQTVELKHDISQLSFNEWDDLDDFETPVKSRVASPVAGTSTKKPSVSDQNTSSSCSSKCEETKVNEESQATETITAPKDVLSAANGVSTETAEREPEDSPIKKSKRPKKSVQHTALLSDTEDEEIIHCVSPDTNQKNFKSVAVAEKDKWGEPNVIDSDDCEENNHYEGFEDFIPPSPIPEEISLSVSDKEKSSSEPVTPANKKESSRSASGLPAPLDQSAKGLKGADDALFSVMESICCLVDTIPEHELIALTCGTELLLQRAHRKRILAKGGSSRTSHSDSVSTPGFLNRPTFGVTPSNLTSLTPVTSGKREGVKTGFSFRKSIASVMSVGDESVFEDSDCIINGVETPGGTWNPNSSTKISAGRDTFNGSIQTLSKPESKTDKCYSRLSFNESSNQTDLFYSPKRVDSGSRNADSSVEINIAGSSSLRTGAEPVDDFLLDDFDIDDFDENDIPDYYEEPPSVLESRNNSGVKTPSVQEGGSSKSFERKTFTPPAPKSIKTPNPEPLYRNPAHDRFRGFNFPHSPEMMKIFHKKFGLHQFRFNQLEAINASLLGEDTFVLMPTGGGKSLCYQLPACVSAGVTVVISPLRSLIVDQVQKLTTLDICATSLSGDKKDSEAARIYMQLSRKDPAIKLLYATPEKVCASGRMISALQNLYERGLLARLVIDEAHCVSQWGHDFRPDYKRLHELRRMFPNVPIMALTATATPRVQKDILNQLAMTRPQVFTMSFNRNNLKYSVLPKKPKKVDEECIQWIKKYYPRDSGIVYCLSRNDCDTLADSLQRAGIAALAYHAGLSDSDREYVQNKWINQDGCQVMCATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCVLFYSYSDVIRIKRLIAMDKDGNQQSKATHINNLHSMVHFCENVAECRRIQLLAYFGEHTFNTSFCKEHPEVICDNCARPNKYKSRNVTDDVKKIARFVQDNCEKVGNRYGKSAQQNRLTLNMLVEIFLGSKSARIQTGMFGVGAAYSKHNAERLFKKLVLDNVLMEDLYITNSGQAVAYISAGPKAMSVLNGCMQVEFHETESASSIRKHRASVCEKVSKREEMVKKCLGELNDLCKKLGKVFGIHYYNIFSTATLKKIAETLSADPEVLLQIDGVTEDKLEKYGAEFIELLQKYSEWQLPAEAQAESSGWIDTTRGHQNDEDDNDDDDEGGGDVTSTYFRSNSGRGAKRKQGSYSRKPKRRKGSSGQNSSAKGGYSSNWSSSRGGGRGGGYRGGSRGAGRGSRSAPSGSAAKRPGFMSLPTPQAAARPFLKPSFSHL

Target ORF information:

RefSeq Version	XM_017352050.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352050.2

ORF Insert Sequence:

ATGTCGAGTC TTCCTCAAAA CAACCTGAAG GAGCATCTGG AGCGACACAA CAATGCTGCC 
CAGAACAAGC TGTCACTGCT CAAACCAAAG CCTGGGGGGT TTTGCTTCAA AAAGAAGTCT 
TCGTCAGGCA TCTCCAAAGT GGAAGTTCCT CAAAAGGTAA CTGGTTCAAG TGTCTTAGCA 
AACAGGAGTG TCAATATTCC CAGCAACTTT GAAGTAACCA AAAATCCTGT GACGTTTTCG 
CAAAAGCCAG AGAGAGTGGC ACCGAAAGTC AACTTCTTCA CTGCGCCCAG CAAACCGAAG 
ACAAATACAG TTAATCCACT AGCAAACCCA TTCGCTTTGA ACAAAACCCC TCTTGTCCAA 
TCTACCATTA AGGCTTTGCC CAAATCTGCA GCCTTGCCTA AGGACTCAAC TAATGATCAA 
ACTGTGGAGC TTAAGCATGA CATTTCTCAA CTCTCATTCA ATGAATGGGA TGACTTGGAT 
GATTTTGAAA CTCCAGTCAA GAGCAGAGTA GCATCCCCAG TTGCAGGGAC CTCTACGAAA 
AAGCCAAGTG TATCAGACCA GAACACATCC TCAAGCTGCT CTTCTAAGTG TGAGGAGACT 
AAAGTAAATG AGGAGTCACA AGCTACAGAA ACCATAACTG CCCCGAAGGA CGTCCTGTCA 
GCTGCTAATG GAGTCAGCAC AGAGACTGCA GAGAGAGAGC CAGAGGACTC ACCCATTAAA 
AAGTCCAAAA GACCTAAGAA ATCAGTCCAG CATACAGCAT TACTTAGTGA CACGGAAGAT 
GAGGAAATAA TCCACTGTGT TTCACCGGAT ACAAACCAGA AGAATTTCAA AAGTGTGGCA 
GTTGCTGAAA AAGATAAATG GGGTGAGCCA AATGTCATAG ACAGTGATGA CTGTGAGGAG 
AACAACCATT ATGAAGGTTT TGAGGATTTC ATCCCCCCGT CCCCTATTCC AGAAGAGATC 
AGTCTCTCTG TCTCAGATAA GGAGAAAAGT TCATCTGAGC CTGTTACTCC TGCTAACAAA 
AAAGAATCTT CTCGATCTGC CTCAGGCCTG CCAGCACCCT TGGATCAATC TGCCAAAGGA 
TTAAAAGGAG CTGATGACGC TCTCTTTAGT GTTATGGAAT CTATCTGTTG TCTAGTGGAC 
ACTATTCCTG AGCATGAGCT CATAGCGCTG ACCTGTGGGA CTGAGCTTTT ACTGCAGAGA 
GCACACAGGA AAAGGATTCT TGCTAAAGGT GGGTCATCTA GAACATCTCA TTCTGACTCA 
GTATCAACTC CTGGTTTCCT AAACAGACCT ACGTTTGGAG TTACACCCTC CAATCTGACA 
TCCCTGACCC CGGTAACTTC AGGAAAAAGA GAAGGTGTCA AAACTGGCTT TTCTTTTCGC 
AAATCCATTG CCTCTGTCAT GTCTGTGGGT GATGAGAGTG TTTTTGAGGA TTCTGACTGC 
ATCATAAATG GAGTCGAAAC ACCTGGTGGT ACCTGGAATC CTAACAGCTC CACTAAAATA 
TCAGCTGGTA GGGATACCTT TAACGGGTCA ATCCAAACTT TAAGCAAACC TGAGTCAAAG 
ACAGATAAAT GCTACTCAAG GTTGTCCTTC AATGAGTCAA GCAATCAGAC TGACCTGTTT 
TATTCCCCCA AGAGAGTGGA TTCAGGCAGC AGAAATGCTG ACAGCAGTGT TGAGATCAAC 
ATTGCAGGAT CCAGCTCTTT ACGAACAGGA GCAGAGCCAG TTGATGATTT TTTACTCGAT 
GACTTCGATA TTGATGATTT TGATGAGAAT GATATTCCAG ATTATTATGA AGAACCTCCA 
AGTGTCTTGG AATCAAGAAA TAACTCTGGT GTAAAAACAC CATCAGTACA GGAGGGAGGA 
TCATCAAAAT CTTTTGAGAG GAAGACATTC ACACCACCAG CACCAAAGTC TATTAAAACT 
CCCAACCCAG AACCTTTGTA CAGGAACCCA GCTCATGACC GCTTCAGAGG CTTTAACTTT 
CCTCATAGCC CAGAGATGAT GAAGATCTTC CATAAGAAGT TTGGACTTCA TCAGTTCCGC 
TTTAACCAGC TGGAAGCTAT TAATGCCTCG CTGTTAGGAG AAGACACATT TGTATTGATG 
CCTACAGGTG GTGGTAAAAG TCTCTGTTAT CAGCTTCCTG CTTGTGTCTC TGCTGGGGTG 
ACTGTAGTCA TTTCTCCTCT ACGCTCTTTA ATAGTTGACC AAGTACAAAA ACTCACCACG 
TTAGATATCT GTGCAACTAG TTTGTCCGGG GACAAAAAAG ATAGTGAGGC TGCCCGAATC 
TACATGCAGC TGTCCAGGAA AGATCCTGCC ATCAAACTGC TTTATGCTAC TCCTGAGAAG 
GTTTGTGCAA GTGGGCGGAT GATCAGTGCA CTTCAGAATC TGTATGAAAG AGGTCTGCTG 
GCTCGCTTGG TCATTGATGA GGCCCATTGC GTCAGTCAGT GGGGTCATGA TTTCAGACCA 
GACTACAAGC GCTTGCATGA GCTCAGGCGG ATGTTTCCCA ATGTTCCTAT AATGGCACTG 
ACGGCCACAG CCACTCCTAG AGTTCAGAAA GACATTCTTA ACCAATTGGC AATGACACGT 
CCTCAGGTAT TTACTATGAG CTTTAACAGG AACAATCTGA AATACTCAGT TTTGCCTAAG 
AAGCCCAAGA AGGTTGATGA GGAATGCATT CAGTGGATTA AGAAATATTA CCCACGTGAC 
TCTGGGATTG TGTACTGCTT GTCTCGTAAT GACTGCGACA CTCTGGCTGA CAGTCTTCAG 
AGGGCAGGGA TCGCAGCGCT GGCGTATCAT GCTGGCCTAA GTGACAGCGA CAGGGAATAT 
GTCCAGAACA AGTGGATCAA CCAGGACGGC TGTCAGGTTA TGTGCGCAAC CATAGCATTT 
GGCATGGGAA TCGATAAGCC TGATGTCCGT TATGTGATCC ATGCGAGTCT CCCCAAATCA 
GTGGAGGGTT ACTACCAGGA GTCAGGCAGA GCAGGGCGAG ATGGAGAGAT TTCCCATTGT 
GTCCTCTTCT ATTCCTACAG TGACGTTATC CGCATCAAGA GACTCATTGC TATGGATAAA 
GATGGCAACC AGCAATCCAA AGCCACCCAC ATCAATAACC TGCACAGCAT GGTGCACTTC 
TGTGAGAATG TGGCTGAATG CAGAAGGATC CAGCTGCTGG CTTACTTTGG GGAGCACACG 
TTTAACACGA GCTTCTGTAA AGAGCATCCT GAGGTCATCT GTGACAACTG TGCCAGACCA 
AACAAATATA AATCCAGAAA TGTGACCGAT GACGTTAAGA AGATTGCGAG GTTTGTGCAG 
GACAACTGTG AGAAGGTTGG AAACAGATAC GGCAAATCCG CCCAGCAGAA CAGGCTCACT 
CTCAACATGC TGGTTGAAAT ATTTCTGGGC TCCAAAAGCG CTCGCATCCA GACTGGGATG 
TTCGGAGTTG GAGCAGCTTA TTCCAAACAC AACGCTGAGA GGCTCTTTAA GAAGCTGGTG 
CTGGATAATG TTCTGATGGA GGATCTGTAC ATCACTAACA GTGGCCAGGC TGTGGCCTAT 
ATCTCTGCAG GCCCTAAAGC TATGAGTGTA CTGAATGGTT GCATGCAGGT TGAATTTCAT 
GAGACCGAGA GCGCATCCAG CATCAGAAAG CACAGAGCGT CTGTGTGTGA GAAGGTGTCT 
AAGAGAGAGG AGATGGTGAA AAAGTGTCTG GGTGAACTTA ATGATCTATG CAAGAAGTTG 
GGCAAAGTCT TCGGCATTCA TTACTACAAT ATCTTCTCCA CCGCTACACT CAAAAAGATT 
GCTGAGACTC TCTCGGCTGA TCCGGAGGTT CTGCTGCAGA TTGATGGTGT AACTGAAGAC 
AAACTGGAGA AATATGGAGC TGAGTTTATT GAACTGCTAC AGAAATACTC TGAGTGGCAG 
CTACCTGCTG AAGCACAGGC TGAGAGTTCA GGCTGGATCG ACACGACCAG AGGCCATCAA 
AATGATGAAG ACGACAACGA TGATGATGAT GAAGGAGGTG GGGATGTCAC ATCGACATAT 
TTCAGAAGCA ACTCTGGACG AGGAGCCAAG AGAAAGCAAG GCTCTTACTC CAGAAAACCC 
AAACGAAGAA AAGGCTCCAG TGGCCAGAAC TCCTCTGCAA AAGGTGGATA TAGCAGTAAC 
TGGTCTTCAT CACGAGGAGG AGGAAGAGGA GGTGGATACA GGGGTGGCAG TCGAGGAGCA 
GGCCGAGGCT CCAGATCTGC TCCTTCAGGC TCAGCAGCAA AAAGGCCGGG CTTCATGTCG 
TTACCAACAC CGCAGGCTGC TGCTCGTCCA TTCCTCAAAC CGTCCTTCTC ACACCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( XM_696265.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_701357.5 Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa55904
Clone ID Related Accession (Same CDS sequence)	XM_696265.6 , XM_696265.7
Accession Version	XM_696265.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Bloom syndrome protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_696265.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAATG AGAGAGCGAG TTGCAAATAC GCGATGTCGA GTCTTCCTCA AAACAACCTG 
AAGGAGCATC TGGAGCGACA CAACAATGCT GCCCAGAACA AGCTGTCACT GCTCAAACCA 
AAGCCTGGGG GGTTTTGCTT CAAAAAGAAG TCTTCGTCAG GCATCTCCAA AGTGGAAGTT 
CCTCAAAAGC CAGAGAGAGT GGCACCGAAA GTCAACTTCT TCACTGCGCC CAGCAAACCG 
AAGACAAATA CAGTTAATCC ACTAGCAAAC CCATTCGCTT TGAACAAAAC CCCTCTTGTC 
CAATCTACCA TTAAGGCTTT GCCCAAATCT GCAGCCTTGC CTAAGGACTC AACTAATGAT 
CAAACTGTGG AGCTTAAGCA TGACATTTCT CAACTCTCAT TCAATGAATG GGATGACTTG 
GATGATTTTG AAACTCCAGT CAAGAGCAGA GTAGCATCCC CAGTTGCAGG GACCTCTACG 
AAAAAGCCAA GTGTATCAGA CCAGAACACA TCCTCAAGCT GCTCTTCTAA GTGTGAGGAG 
ACTAAAGTAA ATGAGGAGTC ACAAGCTACA GAAACCATAA CTGCCCCGAA GGACGTCCTG 
TCAGCTGCTA ATGGAGTCAG CACAGAGACT GCAGAGAGAG AGCCAGAGGA CTCACCCATT 
AAAAAGTCCA AAAGACCTAA GAAATCAGTC CAGCATACAG CATTACTTAG TGACACGGAA 
GATGAGGAAA TAATCCACTG TGTTTCACCG GATACAAACC AGAAGAATTT CAAAAGTGTG 
GCAGTTGCTG AAAAAGATAA ATGGGGTGAG CCAAATGTCA TAGACAGTGA TGACTGTGAG 
GAGAACAACC ATTATGAAGG TTTTGAGGAT TTCATCCCCC CGTCCCCTAT TCCAGAAGAG 
ATCAGTCTCT CTGTCTCAGA TAAGGAGAAA AGTTCATCTG AGCCTGTTAC TCCTGCTAAC 
AAAAAAGAAT CTTCTCGATC TGCCTCAGGC CTGCCAGCAC CCTTGGATCA ATCTGCCAAA 
GGATTAAAAG GAGCTGATGA CGCTCTCTTT AGTGTTATGG AATCTATCTG TTGTCTAGTG 
GACACTATTC CTGAGCATGA GCTCATAGCG CTGACCTGTG GGACTGAGCT TTTACTGCAG 
AGAGCACACA GGAAAAGGAT TCTTGCTAAA GGTGGGTCAT CTAGAACATC TCATTCTGAC 
TCAGTATCAA CTCCTGGTTT CCTAAACAGA CCTACGTTTG GAGTTACACC CTCCAATCTG 
ACATCCCTGA CCCCGGTAAC TTCAGGAAAA AGAGAAGGTG TCAAAACTGG CTTTTCTTTT 
CGCAAATCCA TTGCCTCTGT CATGTCTGTG GGTGATGAGA GTGTTTTTGA GGATTCTGAC 
TGCATCATAA ATGGAGTCGA AACACCTGGT GGTACCTGGA ATCCTAACAG CTCCACTAAA 
ATATCAGCTG GTAGGGATAC CTTTAACGGG TCAATCCAAA CTTTAAGCAA ACCTGAGTCA 
AAGACAGATA AATGCTACTC AAGGTTGTCC TTCAATGAGT CAAGCAATCA GACTGACCTG 
TTTTATTCCC CCAAGAGAGT GGATTCAGGC AGCAGAAATG CTGACAGCAG TGTTGAGATC 
AACATTGCAG GATCCAGCTC TTTACGAACA GGAGCAGAGC CAGTTGATGA TTTTTTACTC 
GATGACTTCG ATATTGATGA TTTTGATGAG AATGATATTC CAGATTATTA TGAAGAACCT 
CCAAGTGTCT TGGAATCAAG AAATAACTCT GGTGTAAAAA CACCATCAGT ACAGGAGGGA 
GGATCATCAA AATCTTTTGA GAGGAAGACA TTCACACCAC CAGCACCAAA GTCTATTAAA 
ACTCCCAACC CAGAACCTTT GTACAGGAAC CCAGCTCATG ACCGCTTCAG AGGCTTTAAC 
TTTCCTCATA GCCCAGAGAT GATGAAGATC TTCCATAAGA AGTTTGGACT TCATCAGTTC 
CGCTTTAACC AGCTGGAAGC TATTAATGCC TCGCTGTTAG GAGAAGACAC ATTTGTATTG 
ATGCCTACAG GTGGTGGTAA AAGTCTCTGT TATCAGCTTC CTGCTTGTGT CTCTGCTGGG 
GTGACTGTAG TCATTTCTCC TCTACGCTCT TTAATAGTTG ACCAAGTACA AAAACTCACC 
ACGTTAGATA TCTGTGCAAC TAGTTTGTCC GGGGACAAAA AAGATAGTGA GGCTGCCCGA 
ATCTACATGC AGCTGTCCAG GAAAGATCCT GCCATCAAAC TGCTTTATGC TACTCCTGAG 
AAGGTTTGTG CAAGTGGGCG GATGATCAGT GCACTTCAGA ATCTGTATGA AAGAGGTCTG 
CTGGCTCGCT TGGTCATTGA TGAGGCCCAT TGCGTCAGTC AGTGGGGTCA TGATTTCAGA 
CCAGACTACA AGCGCTTGCA TGAGCTCAGG CGGATGTTTC CCAATGTTCC TATAATGGCA 
CTGACGGCCA CAGCCACTCC TAGAGTTCAG AAAGACATTC TTAACCAATT GGCAATGACA 
CGTCCTCAGG TATTTACTAT GAGCTTTAAC AGGAACAATC TGAAATACTC AGTTTTGCCT 
AAGAAGCCCA AGAAGGTTGA TGAGGAATGC ATTCAGTGGA TTAAGAAATA TTACCCACGT 
GACTCTGGGA TTGTGTACTG CTTGTCTCGT AATGACTGCG ACACTCTGGC TGACAGTCTT 
CAGAGGGCAG GGATCGCAGC GCTGGCGTAT CATGCTGGCC TAAGTGACAG CGACAGGGAA 
TATGTCCAGA ACAAGTGGAT CAACCAGGAC GGCTGTCAGG TTATGTGCGC AACCATAGCA 
TTTGGCATGG GAATCGATAA GCCTGATGTC CGTTATGTGA TCCATGCGAG TCTCCCCAAA 
TCAGTGGAGG GTTACTACCA GGAGTCAGGC AGAGCAGGGC GAGATGGAGA GATTTCCCAT 
TGTGTCCTCT TCTATTCCTA CAGTGACGTT ATCCGCATCA AGAGACTCAT TGCTATGGAT 
AAAGATGGCA ACCAGCAATC CAAAGCCACC CACATCAATA ACCTGCACAG CATGGTGCAC 
TTCTGTGAGA ATGTGGCTGA ATGCAGAAGG ATCCAGCTGC TGGCTTACTT TGGGGAGCAC 
ACGTTTAACA CGAGCTTCTG TAAAGAGCAT CCTGAGGTCA TCTGTGACAA CTGTGCCAGA 
CCAAACAAAT ATAAATCCAG AAATGTGACC GATGACGTTA AGAAGATTGC GAGGTTTGTG 
CAGGACAACT GTGAGAAGGT TGGAAACAGA TACGGCAAAT CCGCCCAGCA GAACAGGCTC 
ACTCTCAACA TGCTGGTTGA AATATTTCTG GGCTCCAAAA GCGCTCGCAT CCAGACTGGG 
ATGTTCGGAG TTGGAGCAGC TTATTCCAAA CACAACGCTG AGAGGCTCTT TAAGAAGCTG 
GTGCTGGATA ATGTTCTGAT GGAGGATCTG TACATCACTA ACAGTGGCCA GGCTGTGGCC 
TATATCTCTG CAGGCCCTAA AGCTATGAGT GTACTGAATG GTTGCATGCA GGTTGAATTT 
CATGAGACCG AGAGCGCATC CAGCATCAGA AAGCACAGAG CGTCTGTGTG TGAGAAGGTG 
TCTAAGAGAG AGGAGATGGT GAAAAAGTGT CTGGGTGAAC TTAATGATCT ATGCAAGAAG 
TTGGGCAAAG TCTTCGGCAT TCATTACTAC AATATCTTCT CCACCGCTAC ACTCAAAAAG 
ATTGCTGAGA CTCTCTCGGC TGATCCGGAG GTTCTGCTGC AGATTGATGG TGTAACTGAA 
GACAAACTGG AGAAATATGG AGCTGAGTTT ATTGAACTGC TACAGAAATA CTCTGAGTGG 
CAGCTACCTG CTGAAGCACA GGCTGAGAGT TCAGGCTGGA TCGACACGAC CAGAGGCCAT 
CAAAATGATG AAGACGACAA CGATGATGAT GATGAAGGAG GTGGGGATGT CACATCGACA 
TATTTCAGAA GCAACTCTGG ACGAGGAGCC AAGAGAAAGC AAGGCTCTTA CTCCAGAAAA 
CCCAAACGAA GAAAAGGCTC CAGTGGCCAG AACTCCTCTG CAAAAGGTGG ATATAGCAGT 
AACTGGTCTT CATCACGAGG AGGAGGAAGA GGAGGTGGAT ACAGGGGTGG CAGTCGAGGA 
GCAGGCCGAG GCTCCAGATC TGCTCCTTCA GGCTCAGCAG CAAAAAGGCC GGGCTTCATG 
TCGTTACCAA CACCGCAGGC TGCTGCTCGT CCATTCCTCA AACCGTCCTT CTCACACCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_701357.5
CDS	476..4738
Translation	MTNERASCKYAMSSLPQNNLKEHLERHNNAAQNKLSLLKPKPGGFCFKKKSSSGISKVEVPQKPERVAPKVNFFTAPSKPKTNTVNPLANPFALNKTPLVQSTIKALPKSAALPKDSTNDQTVELKHDISQLSFNEWDDLDDFETPVKSRVASPVAGTSTKKPSVSDQNTSSSCSSKCEETKVNEESQATETITAPKDVLSAANGVSTETAEREPEDSPIKKSKRPKKSVQHTALLSDTEDEEIIHCVSPDTNQKNFKSVAVAEKDKWGEPNVIDSDDCEENNHYEGFEDFIPPSPIPEEISLSVSDKEKSSSEPVTPANKKESSRSASGLPAPLDQSAKGLKGADDALFSVMESICCLVDTIPEHELIALTCGTELLLQRAHRKRILAKGGSSRTSHSDSVSTPGFLNRPTFGVTPSNLTSLTPVTSGKREGVKTGFSFRKSIASVMSVGDESVFEDSDCIINGVETPGGTWNPNSSTKISAGRDTFNGSIQTLSKPESKTDKCYSRLSFNESSNQTDLFYSPKRVDSGSRNADSSVEINIAGSSSLRTGAEPVDDFLLDDFDIDDFDENDIPDYYEEPPSVLESRNNSGVKTPSVQEGGSSKSFERKTFTPPAPKSIKTPNPEPLYRNPAHDRFRGFNFPHSPEMMKIFHKKFGLHQFRFNQLEAINASLLGEDTFVLMPTGGGKSLCYQLPACVSAGVTVVISPLRSLIVDQVQKLTTLDICATSLSGDKKDSEAARIYMQLSRKDPAIKLLYATPEKVCASGRMISALQNLYERGLLARLVIDEAHCVSQWGHDFRPDYKRLHELRRMFPNVPIMALTATATPRVQKDILNQLAMTRPQVFTMSFNRNNLKYSVLPKKPKKVDEECIQWIKKYYPRDSGIVYCLSRNDCDTLADSLQRAGIAALAYHAGLSDSDREYVQNKWINQDGCQVMCATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCVLFYSYSDVIRIKRLIAMDKDGNQQSKATHINNLHSMVHFCENVAECRRIQLLAYFGEHTFNTSFCKEHPEVICDNCARPNKYKSRNVTDDVKKIARFVQDNCEKVGNRYGKSAQQNRLTLNMLVEIFLGSKSARIQTGMFGVGAAYSKHNAERLFKKLVLDNVLMEDLYITNSGQAVAYISAGPKAMSVLNGCMQVEFHETESASSIRKHRASVCEKVSKREEMVKKCLGELNDLCKKLGKVFGIHYYNIFSTATLKKIAETLSADPEVLLQIDGVTEDKLEKYGAEFIELLQKYSEWQLPAEAQAESSGWIDTTRGHQNDEDDNDDDDEGGGDVTSTYFRSNSGRGAKRKQGSYSRKPKRRKGSSGQNSSAKGGYSSNWSSSRGGGRGGGYRGGSRGAGRGSRSAPSGSAAKRPGFMSLPTPQAAARPFLKPSFSHL

Target ORF information:

RefSeq Version	XM_696265.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_696265.6

ORF Insert Sequence:

ATGACCAATG AGAGAGCGAG TTGCAAATAC GCGATGTCGA GTCTTCCTCA AAACAACCTG 
AAGGAGCATC TGGAGCGACA CAACAATGCT GCCCAGAACA AGCTGTCACT GCTCAAACCA 
AAGCCTGGGG GGTTTTGCTT CAAAAAGAAG TCTTCGTCAG GCATCTCCAA AGTGGAAGTT 
CCTCAAAAGC CAGAGAGAGT GGCACCGAAA GTCAACTTCT TCACTGCGCC CAGCAAACCG 
AAGACAAATA CAGTTAATCC ACTAGCAAAC CCATTCGCTT TGAACAAAAC CCCTCTTGTC 
CAATCTACCA TTAAGGCTTT GCCCAAATCT GCAGCCTTGC CTAAGGACTC AACTAATGAT 
CAAACTGTGG AGCTTAAGCA TGACATTTCT CAACTCTCAT TCAATGAATG GGATGACTTG 
GATGATTTTG AAACTCCAGT CAAGAGCAGA GTAGCATCCC CAGTTGCAGG GACCTCTACG 
AAAAAGCCAA GTGTATCAGA CCAGAACACA TCCTCAAGCT GCTCTTCTAA GTGTGAGGAG 
ACTAAAGTAA ATGAGGAGTC ACAAGCTACA GAAACCATAA CTGCCCCGAA GGACGTCCTG 
TCAGCTGCTA ATGGAGTCAG CACAGAGACT GCAGAGAGAG AGCCAGAGGA CTCACCCATT 
AAAAAGTCCA AAAGACCTAA GAAATCAGTC CAGCATACAG CATTACTTAG TGACACGGAA 
GATGAGGAAA TAATCCACTG TGTTTCACCG GATACAAACC AGAAGAATTT CAAAAGTGTG 
GCAGTTGCTG AAAAAGATAA ATGGGGTGAG CCAAATGTCA TAGACAGTGA TGACTGTGAG 
GAGAACAACC ATTATGAAGG TTTTGAGGAT TTCATCCCCC CGTCCCCTAT TCCAGAAGAG 
ATCAGTCTCT CTGTCTCAGA TAAGGAGAAA AGTTCATCTG AGCCTGTTAC TCCTGCTAAC 
AAAAAAGAAT CTTCTCGATC TGCCTCAGGC CTGCCAGCAC CCTTGGATCA ATCTGCCAAA 
GGATTAAAAG GAGCTGATGA CGCTCTCTTT AGTGTTATGG AATCTATCTG TTGTCTAGTG 
GACACTATTC CTGAGCATGA GCTCATAGCG CTGACCTGTG GGACTGAGCT TTTACTGCAG 
AGAGCACACA GGAAAAGGAT TCTTGCTAAA GGTGGGTCAT CTAGAACATC TCATTCTGAC 
TCAGTATCAA CTCCTGGTTT CCTAAACAGA CCTACGTTTG GAGTTACACC CTCCAATCTG 
ACATCCCTGA CCCCGGTAAC TTCAGGAAAA AGAGAAGGTG TCAAAACTGG CTTTTCTTTT 
CGCAAATCCA TTGCCTCTGT CATGTCTGTG GGTGATGAGA GTGTTTTTGA GGATTCTGAC 
TGCATCATAA ATGGAGTCGA AACACCTGGT GGTACCTGGA ATCCTAACAG CTCCACTAAA 
ATATCAGCTG GTAGGGATAC CTTTAACGGG TCAATCCAAA CTTTAAGCAA ACCTGAGTCA 
AAGACAGATA AATGCTACTC AAGGTTGTCC TTCAATGAGT CAAGCAATCA GACTGACCTG 
TTTTATTCCC CCAAGAGAGT GGATTCAGGC AGCAGAAATG CTGACAGCAG TGTTGAGATC 
AACATTGCAG GATCCAGCTC TTTACGAACA GGAGCAGAGC CAGTTGATGA TTTTTTACTC 
GATGACTTCG ATATTGATGA TTTTGATGAG AATGATATTC CAGATTATTA TGAAGAACCT 
CCAAGTGTCT TGGAATCAAG AAATAACTCT GGTGTAAAAA CACCATCAGT ACAGGAGGGA 
GGATCATCAA AATCTTTTGA GAGGAAGACA TTCACACCAC CAGCACCAAA GTCTATTAAA 
ACTCCCAACC CAGAACCTTT GTACAGGAAC CCAGCTCATG ACCGCTTCAG AGGCTTTAAC 
TTTCCTCATA GCCCAGAGAT GATGAAGATC TTCCATAAGA AGTTTGGACT TCATCAGTTC 
CGCTTTAACC AGCTGGAAGC TATTAATGCC TCGCTGTTAG GAGAAGACAC ATTTGTATTG 
ATGCCTACAG GTGGTGGTAA AAGTCTCTGT TATCAGCTTC CTGCTTGTGT CTCTGCTGGG 
GTGACTGTAG TCATTTCTCC TCTACGCTCT TTAATAGTTG ACCAAGTACA AAAACTCACC 
ACGTTAGATA TCTGTGCAAC TAGTTTGTCC GGGGACAAAA AAGATAGTGA GGCTGCCCGA 
ATCTACATGC AGCTGTCCAG GAAAGATCCT GCCATCAAAC TGCTTTATGC TACTCCTGAG 
AAGGTTTGTG CAAGTGGGCG GATGATCAGT GCACTTCAGA ATCTGTATGA AAGAGGTCTG 
CTGGCTCGCT TGGTCATTGA TGAGGCCCAT TGCGTCAGTC AGTGGGGTCA TGATTTCAGA 
CCAGACTACA AGCGCTTGCA TGAGCTCAGG CGGATGTTTC CCAATGTTCC TATAATGGCA 
CTGACGGCCA CAGCCACTCC TAGAGTTCAG AAAGACATTC TTAACCAATT GGCAATGACA 
CGTCCTCAGG TATTTACTAT GAGCTTTAAC AGGAACAATC TGAAATACTC AGTTTTGCCT 
AAGAAGCCCA AGAAGGTTGA TGAGGAATGC ATTCAGTGGA TTAAGAAATA TTACCCACGT 
GACTCTGGGA TTGTGTACTG CTTGTCTCGT AATGACTGCG ACACTCTGGC TGACAGTCTT 
CAGAGGGCAG GGATCGCAGC GCTGGCGTAT CATGCTGGCC TAAGTGACAG CGACAGGGAA 
TATGTCCAGA ACAAGTGGAT CAACCAGGAC GGCTGTCAGG TTATGTGCGC AACCATAGCA 
TTTGGCATGG GAATCGATAA GCCTGATGTC CGTTATGTGA TCCATGCGAG TCTCCCCAAA 
TCAGTGGAGG GTTACTACCA GGAGTCAGGC AGAGCAGGGC GAGATGGAGA GATTTCCCAT 
TGTGTCCTCT TCTATTCCTA CAGTGACGTT ATCCGCATCA AGAGACTCAT TGCTATGGAT 
AAAGATGGCA ACCAGCAATC CAAAGCCACC CACATCAATA ACCTGCACAG CATGGTGCAC 
TTCTGTGAGA ATGTGGCTGA ATGCAGAAGG ATCCAGCTGC TGGCTTACTT TGGGGAGCAC 
ACGTTTAACA CGAGCTTCTG TAAAGAGCAT CCTGAGGTCA TCTGTGACAA CTGTGCCAGA 
CCAAACAAAT ATAAATCCAG AAATGTGACC GATGACGTTA AGAAGATTGC GAGGTTTGTG 
CAGGACAACT GTGAGAAGGT TGGAAACAGA TACGGCAAAT CCGCCCAGCA GAACAGGCTC 
ACTCTCAACA TGCTGGTTGA AATATTTCTG GGCTCCAAAA GCGCTCGCAT CCAGACTGGG 
ATGTTCGGAG TTGGAGCAGC TTATTCCAAA CACAACGCTG AGAGGCTCTT TAAGAAGCTG 
GTGCTGGATA ATGTTCTGAT GGAGGATCTG TACATCACTA ACAGTGGCCA GGCTGTGGCC 
TATATCTCTG CAGGCCCTAA AGCTATGAGT GTACTGAATG GTTGCATGCA GGTTGAATTT 
CATGAGACCG AGAGCGCATC CAGCATCAGA AAGCACAGAG CGTCTGTGTG TGAGAAGGTG 
TCTAAGAGAG AGGAGATGGT GAAAAAGTGT CTGGGTGAAC TTAATGATCT ATGCAAGAAG 
TTGGGCAAAG TCTTCGGCAT TCATTACTAC AATATCTTCT CCACCGCTAC ACTCAAAAAG 
ATTGCTGAGA CTCTCTCGGC TGATCCGGAG GTTCTGCTGC AGATTGATGG TGTAACTGAA 
GACAAACTGG AGAAATATGG AGCTGAGTTT ATTGAACTGC TACAGAAATA CTCTGAGTGG 
CAGCTACCTG CTGAAGCACA GGCTGAGAGT TCAGGCTGGA TCGACACGAC CAGAGGCCAT 
CAAAATGATG AAGACGACAA CGATGATGAT GATGAAGGAG GTGGGGATGT CACATCGACA 
TATTTCAGAA GCAACTCTGG ACGAGGAGCC AAGAGAAAGC AAGGCTCTTA CTCCAGAAAA 
CCCAAACGAA GAAAAGGCTC CAGTGGCCAG AACTCCTCTG CAAAAGGTGG ATATAGCAGT 
AACTGGTCTT CATCACGAGG AGGAGGAAGA GGAGGTGGAT ACAGGGGTGG CAGTCGAGGA 
GCAGGCCGAG GCTCCAGATC TGCTCCTTCA GGCTCAGCAG CAAAAAGGCC GGGCTTCATG 
TCGTTACCAA CACCGCAGGC TGCTGCTCGT CCATTCCTCA AACCGTCCTT CTCACACCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( XM_696265.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_701357.5 Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X4, mRNA.

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CloneID	ODa55904
Clone ID Related Accession (Same CDS sequence)	XM_696265.6 , XM_696265.7
Accession Version	XM_696265.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Bloom syndrome protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_696265.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAATG AGAGAGCGAG TTGCAAATAC GCGATGTCGA GTCTTCCTCA AAACAACCTG 
AAGGAGCATC TGGAGCGACA CAACAATGCT GCCCAGAACA AGCTGTCACT GCTCAAACCA 
AAGCCTGGGG GGTTTTGCTT CAAAAAGAAG TCTTCGTCAG GCATCTCCAA AGTGGAAGTT 
CCTCAAAAGC CAGAGAGAGT GGCACCGAAA GTCAACTTCT TCACTGCGCC CAGCAAACCG 
AAGACAAATA CAGTTAATCC ACTAGCAAAC CCATTCGCTT TGAACAAAAC CCCTCTTGTC 
CAATCTACCA TTAAGGCTTT GCCCAAATCT GCAGCCTTGC CTAAGGACTC AACTAATGAT 
CAAACTGTGG AGCTTAAGCA TGACATTTCT CAACTCTCAT TCAATGAATG GGATGACTTG 
GATGATTTTG AAACTCCAGT CAAGAGCAGA GTAGCATCCC CAGTTGCAGG GACCTCTACG 
AAAAAGCCAA GTGTATCAGA CCAGAACACA TCCTCAAGCT GCTCTTCTAA GTGTGAGGAG 
ACTAAAGTAA ATGAGGAGTC ACAAGCTACA GAAACCATAA CTGCCCCGAA GGACGTCCTG 
TCAGCTGCTA ATGGAGTCAG CACAGAGACT GCAGAGAGAG AGCCAGAGGA CTCACCCATT 
AAAAAGTCCA AAAGACCTAA GAAATCAGTC CAGCATACAG CATTACTTAG TGACACGGAA 
GATGAGGAAA TAATCCACTG TGTTTCACCG GATACAAACC AGAAGAATTT CAAAAGTGTG 
GCAGTTGCTG AAAAAGATAA ATGGGGTGAG CCAAATGTCA TAGACAGTGA TGACTGTGAG 
GAGAACAACC ATTATGAAGG TTTTGAGGAT TTCATCCCCC CGTCCCCTAT TCCAGAAGAG 
ATCAGTCTCT CTGTCTCAGA TAAGGAGAAA AGTTCATCTG AGCCTGTTAC TCCTGCTAAC 
AAAAAAGAAT CTTCTCGATC TGCCTCAGGC CTGCCAGCAC CCTTGGATCA ATCTGCCAAA 
GGATTAAAAG GAGCTGATGA CGCTCTCTTT AGTGTTATGG AATCTATCTG TTGTCTAGTG 
GACACTATTC CTGAGCATGA GCTCATAGCG CTGACCTGTG GGACTGAGCT TTTACTGCAG 
AGAGCACACA GGAAAAGGAT TCTTGCTAAA GGTGGGTCAT CTAGAACATC TCATTCTGAC 
TCAGTATCAA CTCCTGGTTT CCTAAACAGA CCTACGTTTG GAGTTACACC CTCCAATCTG 
ACATCCCTGA CCCCGGTAAC TTCAGGAAAA AGAGAAGGTG TCAAAACTGG CTTTTCTTTT 
CGCAAATCCA TTGCCTCTGT CATGTCTGTG GGTGATGAGA GTGTTTTTGA GGATTCTGAC 
TGCATCATAA ATGGAGTCGA AACACCTGGT GGTACCTGGA ATCCTAACAG CTCCACTAAA 
ATATCAGCTG GTAGGGATAC CTTTAACGGG TCAATCCAAA CTTTAAGCAA ACCTGAGTCA 
AAGACAGATA AATGCTACTC AAGGTTGTCC TTCAATGAGT CAAGCAATCA GACTGACCTG 
TTTTATTCCC CCAAGAGAGT GGATTCAGGC AGCAGAAATG CTGACAGCAG TGTTGAGATC 
AACATTGCAG GATCCAGCTC TTTACGAACA GGAGCAGAGC CAGTTGATGA TTTTTTACTC 
GATGACTTCG ATATTGATGA TTTTGATGAG AATGATATTC CAGATTATTA TGAAGAACCT 
CCAAGTGTCT TGGAATCAAG AAATAACTCT GGTGTAAAAA CACCATCAGT ACAGGAGGGA 
GGATCATCAA AATCTTTTGA GAGGAAGACA TTCACACCAC CAGCACCAAA GTCTATTAAA 
ACTCCCAACC CAGAACCTTT GTACAGGAAC CCAGCTCATG ACCGCTTCAG AGGCTTTAAC 
TTTCCTCATA GCCCAGAGAT GATGAAGATC TTCCATAAGA AGTTTGGACT TCATCAGTTC 
CGCTTTAACC AGCTGGAAGC TATTAATGCC TCGCTGTTAG GAGAAGACAC ATTTGTATTG 
ATGCCTACAG GTGGTGGTAA AAGTCTCTGT TATCAGCTTC CTGCTTGTGT CTCTGCTGGG 
GTGACTGTAG TCATTTCTCC TCTACGCTCT TTAATAGTTG ACCAAGTACA AAAACTCACC 
ACGTTAGATA TCTGTGCAAC TAGTTTGTCC GGGGACAAAA AAGATAGTGA GGCTGCCCGA 
ATCTACATGC AGCTGTCCAG GAAAGATCCT GCCATCAAAC TGCTTTATGC TACTCCTGAG 
AAGGTTTGTG CAAGTGGGCG GATGATCAGT GCACTTCAGA ATCTGTATGA AAGAGGTCTG 
CTGGCTCGCT TGGTCATTGA TGAGGCCCAT TGCGTCAGTC AGTGGGGTCA TGATTTCAGA 
CCAGACTACA AGCGCTTGCA TGAGCTCAGG CGGATGTTTC CCAATGTTCC TATAATGGCA 
CTGACGGCCA CAGCCACTCC TAGAGTTCAG AAAGACATTC TTAACCAATT GGCAATGACA 
CGTCCTCAGG TATTTACTAT GAGCTTTAAC AGGAACAATC TGAAATACTC AGTTTTGCCT 
AAGAAGCCCA AGAAGGTTGA TGAGGAATGC ATTCAGTGGA TTAAGAAATA TTACCCACGT 
GACTCTGGGA TTGTGTACTG CTTGTCTCGT AATGACTGCG ACACTCTGGC TGACAGTCTT 
CAGAGGGCAG GGATCGCAGC GCTGGCGTAT CATGCTGGCC TAAGTGACAG CGACAGGGAA 
TATGTCCAGA ACAAGTGGAT CAACCAGGAC GGCTGTCAGG TTATGTGCGC AACCATAGCA 
TTTGGCATGG GAATCGATAA GCCTGATGTC CGTTATGTGA TCCATGCGAG TCTCCCCAAA 
TCAGTGGAGG GTTACTACCA GGAGTCAGGC AGAGCAGGGC GAGATGGAGA GATTTCCCAT 
TGTGTCCTCT TCTATTCCTA CAGTGACGTT ATCCGCATCA AGAGACTCAT TGCTATGGAT 
AAAGATGGCA ACCAGCAATC CAAAGCCACC CACATCAATA ACCTGCACAG CATGGTGCAC 
TTCTGTGAGA ATGTGGCTGA ATGCAGAAGG ATCCAGCTGC TGGCTTACTT TGGGGAGCAC 
ACGTTTAACA CGAGCTTCTG TAAAGAGCAT CCTGAGGTCA TCTGTGACAA CTGTGCCAGA 
CCAAACAAAT ATAAATCCAG AAATGTGACC GATGACGTTA AGAAGATTGC GAGGTTTGTG 
CAGGACAACT GTGAGAAGGT TGGAAACAGA TACGGCAAAT CCGCCCAGCA GAACAGGCTC 
ACTCTCAACA TGCTGGTTGA AATATTTCTG GGCTCCAAAA GCGCTCGCAT CCAGACTGGG 
ATGTTCGGAG TTGGAGCAGC TTATTCCAAA CACAACGCTG AGAGGCTCTT TAAGAAGCTG 
GTGCTGGATA ATGTTCTGAT GGAGGATCTG TACATCACTA ACAGTGGCCA GGCTGTGGCC 
TATATCTCTG CAGGCCCTAA AGCTATGAGT GTACTGAATG GTTGCATGCA GGTTGAATTT 
CATGAGACCG AGAGCGCATC CAGCATCAGA AAGCACAGAG CGTCTGTGTG TGAGAAGGTG 
TCTAAGAGAG AGGAGATGGT GAAAAAGTGT CTGGGTGAAC TTAATGATCT ATGCAAGAAG 
TTGGGCAAAG TCTTCGGCAT TCATTACTAC AATATCTTCT CCACCGCTAC ACTCAAAAAG 
ATTGCTGAGA CTCTCTCGGC TGATCCGGAG GTTCTGCTGC AGATTGATGG TGTAACTGAA 
GACAAACTGG AGAAATATGG AGCTGAGTTT ATTGAACTGC TACAGAAATA CTCTGAGTGG 
CAGCTACCTG CTGAAGCACA GGCTGAGAGT TCAGGCTGGA TCGACACGAC CAGAGGCCAT 
CAAAATGATG AAGACGACAA CGATGATGAT GATGAAGGAG GTGGGGATGT CACATCGACA 
TATTTCAGAA GCAACTCTGG ACGAGGAGCC AAGAGAAAGC AAGGCTCTTA CTCCAGAAAA 
CCCAAACGAA GAAAAGGCTC CAGTGGCCAG AACTCCTCTG CAAAAGGTGG ATATAGCAGT 
AACTGGTCTT CATCACGAGG AGGAGGAAGA GGAGGTGGAT ACAGGGGTGG CAGTCGAGGA 
GCAGGCCGAG GCTCCAGATC TGCTCCTTCA GGCTCAGCAG CAAAAAGGCC GGGCTTCATG 
TCGTTACCAA CACCGCAGGC TGCTGCTCGT CCATTCCTCA AACCGTCCTT CTCACACCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_701357.5
CDS	332..4594
Translation	MTNERASCKYAMSSLPQNNLKEHLERHNNAAQNKLSLLKPKPGGFCFKKKSSSGISKVEVPQKPERVAPKVNFFTAPSKPKTNTVNPLANPFALNKTPLVQSTIKALPKSAALPKDSTNDQTVELKHDISQLSFNEWDDLDDFETPVKSRVASPVAGTSTKKPSVSDQNTSSSCSSKCEETKVNEESQATETITAPKDVLSAANGVSTETAEREPEDSPIKKSKRPKKSVQHTALLSDTEDEEIIHCVSPDTNQKNFKSVAVAEKDKWGEPNVIDSDDCEENNHYEGFEDFIPPSPIPEEISLSVSDKEKSSSEPVTPANKKESSRSASGLPAPLDQSAKGLKGADDALFSVMESICCLVDTIPEHELIALTCGTELLLQRAHRKRILAKGGSSRTSHSDSVSTPGFLNRPTFGVTPSNLTSLTPVTSGKREGVKTGFSFRKSIASVMSVGDESVFEDSDCIINGVETPGGTWNPNSSTKISAGRDTFNGSIQTLSKPESKTDKCYSRLSFNESSNQTDLFYSPKRVDSGSRNADSSVEINIAGSSSLRTGAEPVDDFLLDDFDIDDFDENDIPDYYEEPPSVLESRNNSGVKTPSVQEGGSSKSFERKTFTPPAPKSIKTPNPEPLYRNPAHDRFRGFNFPHSPEMMKIFHKKFGLHQFRFNQLEAINASLLGEDTFVLMPTGGGKSLCYQLPACVSAGVTVVISPLRSLIVDQVQKLTTLDICATSLSGDKKDSEAARIYMQLSRKDPAIKLLYATPEKVCASGRMISALQNLYERGLLARLVIDEAHCVSQWGHDFRPDYKRLHELRRMFPNVPIMALTATATPRVQKDILNQLAMTRPQVFTMSFNRNNLKYSVLPKKPKKVDEECIQWIKKYYPRDSGIVYCLSRNDCDTLADSLQRAGIAALAYHAGLSDSDREYVQNKWINQDGCQVMCATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCVLFYSYSDVIRIKRLIAMDKDGNQQSKATHINNLHSMVHFCENVAECRRIQLLAYFGEHTFNTSFCKEHPEVICDNCARPNKYKSRNVTDDVKKIARFVQDNCEKVGNRYGKSAQQNRLTLNMLVEIFLGSKSARIQTGMFGVGAAYSKHNAERLFKKLVLDNVLMEDLYITNSGQAVAYISAGPKAMSVLNGCMQVEFHETESASSIRKHRASVCEKVSKREEMVKKCLGELNDLCKKLGKVFGIHYYNIFSTATLKKIAETLSADPEVLLQIDGVTEDKLEKYGAEFIELLQKYSEWQLPAEAQAESSGWIDTTRGHQNDEDDNDDDDEGGGDVTSTYFRSNSGRGAKRKQGSYSRKPKRRKGSSGQNSSAKGGYSSNWSSSRGGGRGGGYRGGSRGAGRGSRSAPSGSAAKRPGFMSLPTPQAAARPFLKPSFSHL

Target ORF information:

RefSeq Version	XM_696265.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Bloom syndrome, RecQ helicase-like (blm), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_696265.7

ORF Insert Sequence:

ATGACCAATG AGAGAGCGAG TTGCAAATAC GCGATGTCGA GTCTTCCTCA AAACAACCTG 
AAGGAGCATC TGGAGCGACA CAACAATGCT GCCCAGAACA AGCTGTCACT GCTCAAACCA 
AAGCCTGGGG GGTTTTGCTT CAAAAAGAAG TCTTCGTCAG GCATCTCCAA AGTGGAAGTT 
CCTCAAAAGC CAGAGAGAGT GGCACCGAAA GTCAACTTCT TCACTGCGCC CAGCAAACCG 
AAGACAAATA CAGTTAATCC ACTAGCAAAC CCATTCGCTT TGAACAAAAC CCCTCTTGTC 
CAATCTACCA TTAAGGCTTT GCCCAAATCT GCAGCCTTGC CTAAGGACTC AACTAATGAT 
CAAACTGTGG AGCTTAAGCA TGACATTTCT CAACTCTCAT TCAATGAATG GGATGACTTG 
GATGATTTTG AAACTCCAGT CAAGAGCAGA GTAGCATCCC CAGTTGCAGG GACCTCTACG 
AAAAAGCCAA GTGTATCAGA CCAGAACACA TCCTCAAGCT GCTCTTCTAA GTGTGAGGAG 
ACTAAAGTAA ATGAGGAGTC ACAAGCTACA GAAACCATAA CTGCCCCGAA GGACGTCCTG 
TCAGCTGCTA ATGGAGTCAG CACAGAGACT GCAGAGAGAG AGCCAGAGGA CTCACCCATT 
AAAAAGTCCA AAAGACCTAA GAAATCAGTC CAGCATACAG CATTACTTAG TGACACGGAA 
GATGAGGAAA TAATCCACTG TGTTTCACCG GATACAAACC AGAAGAATTT CAAAAGTGTG 
GCAGTTGCTG AAAAAGATAA ATGGGGTGAG CCAAATGTCA TAGACAGTGA TGACTGTGAG 
GAGAACAACC ATTATGAAGG TTTTGAGGAT TTCATCCCCC CGTCCCCTAT TCCAGAAGAG 
ATCAGTCTCT CTGTCTCAGA TAAGGAGAAA AGTTCATCTG AGCCTGTTAC TCCTGCTAAC 
AAAAAAGAAT CTTCTCGATC TGCCTCAGGC CTGCCAGCAC CCTTGGATCA ATCTGCCAAA 
GGATTAAAAG GAGCTGATGA CGCTCTCTTT AGTGTTATGG AATCTATCTG TTGTCTAGTG 
GACACTATTC CTGAGCATGA GCTCATAGCG CTGACCTGTG GGACTGAGCT TTTACTGCAG 
AGAGCACACA GGAAAAGGAT TCTTGCTAAA GGTGGGTCAT CTAGAACATC TCATTCTGAC 
TCAGTATCAA CTCCTGGTTT CCTAAACAGA CCTACGTTTG GAGTTACACC CTCCAATCTG 
ACATCCCTGA CCCCGGTAAC TTCAGGAAAA AGAGAAGGTG TCAAAACTGG CTTTTCTTTT 
CGCAAATCCA TTGCCTCTGT CATGTCTGTG GGTGATGAGA GTGTTTTTGA GGATTCTGAC 
TGCATCATAA ATGGAGTCGA AACACCTGGT GGTACCTGGA ATCCTAACAG CTCCACTAAA 
ATATCAGCTG GTAGGGATAC CTTTAACGGG TCAATCCAAA CTTTAAGCAA ACCTGAGTCA 
AAGACAGATA AATGCTACTC AAGGTTGTCC TTCAATGAGT CAAGCAATCA GACTGACCTG 
TTTTATTCCC CCAAGAGAGT GGATTCAGGC AGCAGAAATG CTGACAGCAG TGTTGAGATC 
AACATTGCAG GATCCAGCTC TTTACGAACA GGAGCAGAGC CAGTTGATGA TTTTTTACTC 
GATGACTTCG ATATTGATGA TTTTGATGAG AATGATATTC CAGATTATTA TGAAGAACCT 
CCAAGTGTCT TGGAATCAAG AAATAACTCT GGTGTAAAAA CACCATCAGT ACAGGAGGGA 
GGATCATCAA AATCTTTTGA GAGGAAGACA TTCACACCAC CAGCACCAAA GTCTATTAAA 
ACTCCCAACC CAGAACCTTT GTACAGGAAC CCAGCTCATG ACCGCTTCAG AGGCTTTAAC 
TTTCCTCATA GCCCAGAGAT GATGAAGATC TTCCATAAGA AGTTTGGACT TCATCAGTTC 
CGCTTTAACC AGCTGGAAGC TATTAATGCC TCGCTGTTAG GAGAAGACAC ATTTGTATTG 
ATGCCTACAG GTGGTGGTAA AAGTCTCTGT TATCAGCTTC CTGCTTGTGT CTCTGCTGGG 
GTGACTGTAG TCATTTCTCC TCTACGCTCT TTAATAGTTG ACCAAGTACA AAAACTCACC 
ACGTTAGATA TCTGTGCAAC TAGTTTGTCC GGGGACAAAA AAGATAGTGA GGCTGCCCGA 
ATCTACATGC AGCTGTCCAG GAAAGATCCT GCCATCAAAC TGCTTTATGC TACTCCTGAG 
AAGGTTTGTG CAAGTGGGCG GATGATCAGT GCACTTCAGA ATCTGTATGA AAGAGGTCTG 
CTGGCTCGCT TGGTCATTGA TGAGGCCCAT TGCGTCAGTC AGTGGGGTCA TGATTTCAGA 
CCAGACTACA AGCGCTTGCA TGAGCTCAGG CGGATGTTTC CCAATGTTCC TATAATGGCA 
CTGACGGCCA CAGCCACTCC TAGAGTTCAG AAAGACATTC TTAACCAATT GGCAATGACA 
CGTCCTCAGG TATTTACTAT GAGCTTTAAC AGGAACAATC TGAAATACTC AGTTTTGCCT 
AAGAAGCCCA AGAAGGTTGA TGAGGAATGC ATTCAGTGGA TTAAGAAATA TTACCCACGT 
GACTCTGGGA TTGTGTACTG CTTGTCTCGT AATGACTGCG ACACTCTGGC TGACAGTCTT 
CAGAGGGCAG GGATCGCAGC GCTGGCGTAT CATGCTGGCC TAAGTGACAG CGACAGGGAA 
TATGTCCAGA ACAAGTGGAT CAACCAGGAC GGCTGTCAGG TTATGTGCGC AACCATAGCA 
TTTGGCATGG GAATCGATAA GCCTGATGTC CGTTATGTGA TCCATGCGAG TCTCCCCAAA 
TCAGTGGAGG GTTACTACCA GGAGTCAGGC AGAGCAGGGC GAGATGGAGA GATTTCCCAT 
TGTGTCCTCT TCTATTCCTA CAGTGACGTT ATCCGCATCA AGAGACTCAT TGCTATGGAT 
AAAGATGGCA ACCAGCAATC CAAAGCCACC CACATCAATA ACCTGCACAG CATGGTGCAC 
TTCTGTGAGA ATGTGGCTGA ATGCAGAAGG ATCCAGCTGC TGGCTTACTT TGGGGAGCAC 
ACGTTTAACA CGAGCTTCTG TAAAGAGCAT CCTGAGGTCA TCTGTGACAA CTGTGCCAGA 
CCAAACAAAT ATAAATCCAG AAATGTGACC GATGACGTTA AGAAGATTGC GAGGTTTGTG 
CAGGACAACT GTGAGAAGGT TGGAAACAGA TACGGCAAAT CCGCCCAGCA GAACAGGCTC 
ACTCTCAACA TGCTGGTTGA AATATTTCTG GGCTCCAAAA GCGCTCGCAT CCAGACTGGG 
ATGTTCGGAG TTGGAGCAGC TTATTCCAAA CACAACGCTG AGAGGCTCTT TAAGAAGCTG 
GTGCTGGATA ATGTTCTGAT GGAGGATCTG TACATCACTA ACAGTGGCCA GGCTGTGGCC 
TATATCTCTG CAGGCCCTAA AGCTATGAGT GTACTGAATG GTTGCATGCA GGTTGAATTT 
CATGAGACCG AGAGCGCATC CAGCATCAGA AAGCACAGAG CGTCTGTGTG TGAGAAGGTG 
TCTAAGAGAG AGGAGATGGT GAAAAAGTGT CTGGGTGAAC TTAATGATCT ATGCAAGAAG 
TTGGGCAAAG TCTTCGGCAT TCATTACTAC AATATCTTCT CCACCGCTAC ACTCAAAAAG 
ATTGCTGAGA CTCTCTCGGC TGATCCGGAG GTTCTGCTGC AGATTGATGG TGTAACTGAA 
GACAAACTGG AGAAATATGG AGCTGAGTTT ATTGAACTGC TACAGAAATA CTCTGAGTGG 
CAGCTACCTG CTGAAGCACA GGCTGAGAGT TCAGGCTGGA TCGACACGAC CAGAGGCCAT 
CAAAATGATG AAGACGACAA CGATGATGAT GATGAAGGAG GTGGGGATGT CACATCGACA 
TATTTCAGAA GCAACTCTGG ACGAGGAGCC AAGAGAAAGC AAGGCTCTTA CTCCAGAAAA 
CCCAAACGAA GAAAAGGCTC CAGTGGCCAG AACTCCTCTG CAAAAGGTGG ATATAGCAGT 
AACTGGTCTT CATCACGAGG AGGAGGAAGA GGAGGTGGAT ACAGGGGTGG CAGTCGAGGA 
GCAGGCCGAG GCTCCAGATC TGCTCCTTCA GGCTCAGCAG CAAAAAGGCC GGGCTTCATG 
TCGTTACCAA CACCGCAGGC TGCTGCTCGT CCATTCCTCA AACCGTCCTT CTCACACCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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BLM cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

サポート

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