BLM cDNA ORF clone, Bos taurus(cattle)

The following BLM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BLM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa228682 XM_002696583.6
Latest version!
Bos taurus Bloom syndrome RecQ like helicase (BLM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa228683 XM_010816994.3
Latest version!
Bos taurus Bloom syndrome RecQ like helicase (BLM), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa52650 XM_010816994.2
Latest version!
Bos taurus Bloom syndrome, RecQ helicase-like (BLM), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa52650 XM_010824828.2
Latest version!
Bos taurus Bloom syndrome, RecQ helicase-like (BLM), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa30869 XM_002696583.5
Latest version!
Bos taurus Bloom syndrome, RecQ helicase-like (BLM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa30869 XM_613809.7
Latest version!
Bos taurus Bloom syndrome, RecQ helicase-like (BLM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa228682
    Clone ID Related Accession (Same CDS sequence) XM_002696583.6
    Accession Version XM_002696583.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4293bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product Bloom syndrome protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002696583.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAACAACTGG AACGTCATTC GGCAAGAAAG 
    CTTAACAATA AATTGAGTCT TTCAAAACCG AAATCTTCAG GTTTCACTTT TAAAAAGAAA
    GTGCCTTCAG CCAACGATGT CTCTGTAACT AGTGTGTCAG TGGCAAAAAC ACCTGTGTTG
    AGTGATAAAG ATGTTAATAT CACTGAGGCC TTTTCCTTCA GTGAACCTTT ACCCCACACC
    ACCAATCAGC AGACGGGGAT CAATGACTTT AAAAGTGCTC CATCCGGACC GCAAACTAAG
    AGAGTTGGTT CAAAACCATT ATTGCCAAGT TTGTCGCAGG TTCCCCAGGA AGTTTCATGT
    ACGGCCCAGA ACACACCGGT TATAAAGCAA TCCCTCAATG CTACTTTCAA GAAATTAGAA
    TTTAGTTCCT CATCAGATTC TCTTATTGCC TTCAATGATT GGGATGATAT GGATGACTTC
    GATACTTCTG GAAATTCTAA AGCATTTGTG ACACCATGTA AAAACCACTT CGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGTCTAAG AGGAACTTAG TAAAAGCGCA GCTTAATAAA
    ACACATACAG GGGAGACTGA TTTGGCTCCC TTCTCCTCTG AGAGCAAGCA GGAACATTTG
    ACTGAGAAAC AGAAGGATGA CTCAGAATGG TTAAGTAGTG ATGTGATTTG CATTGATGAT
    GACCCCGATC CCGAAGAGCT GATAAATGAA GATACTCAGG AAAATCACTC GTTAAAAACT
    CATTTGGGAG CGGAAAGAGG TAATTGTGAA AAGAAGAAAA ACTTGGAAGA AACAGAATTA
    CGTTCAGTGG AGAAGTCTTC ATGTATTGAG CTTGACGAAG ATGATTATGA CATAGATTTT
    GTCCCACCTT CTCCAGAAGA AGGAATGACT TCTACCTCCT CATCCTCTTT AAAATGCTCT
    AGTATGTTGA AGGATCTTGA CACCTCTGAC AAAAAGCATA GTCTTAGCAC ATCAGAAGAC
    TTGTCAAAAC TCAAGGAAAT GGCCACACAA CAGCCTAATC AAGAAACCAG CACAAGCTGT
    GAAGCTAGAC AGACAAGTTT ACAGCAGCAG CTTATCCGTG TGATGGACCG AATCTGTAAA
    TTAGTTGATG CTGTTCCTGA TGATGAGCTG AAAGCTTTGG ACTGTGGGAA CGAGCTGCTC
    CAGCAGCGGG ACATAAGAAG GAAACTCCTA GCTGAAGCGG ATTTTGATCC CAGTGATGCC
    AGTGTTCTTA GCGCCATGTG GGGGTGTAGG CCTGATCCCC TGGAGAGCCC TGTGAAGGAC
    TTGGCCTCTG TTTCCTTACC TTACGCGTTT CGGAAGGGTG ACTCCTGTCT TGCAGGGAAT
    GCTGTGAAGG AGTTTACTCT TCCACACCTC CCCTCAAATT CCATGTCCAC CCGGGAACGT
    TTACTGACTC CTCCCCTAGG AAAGACAGGA GTCTTAGCCA CCACAAAGAG TCCCTTTGAA
    AGACCGCTAT TCAGTTCCCA TTTACAGAAG TCCTTTGTAA GTAGCAACTG GGCTGAAACA
    CCGAGGGCAG AGAAAAGAAA TGAAAGCCCT GATTTCCCAG GAAATGTTCT CACAAGCACG
    GCTGTGAAAG ATCAGAATAA ATATACCACC TCCATAAATG ACTTGGAAAG AGAGCTCCAG
    GCTGCTTGCG AGATTGACAA TTTTGACATA GATGACTTTG ACGATGATGA TGATGACTGG
    GAAAATCTGA TGCAAAATTT AGCAGCCAGC AAATCTTCCA CAGCTGCCTA CCCACCCATT
    AAGGAAGGTC GACCAGTTAA ATCTGTGTCA GAAAGACCCT CTTCCGCCAA GACAGACTGC
    CTTCCAGGGG CATCTACCAC TCAAAATAAA AACTTCTCAG AGTCAATTCC GAATTACCCT
    GACAAGATGG AGCAGAATTT AGCATCCAGA AATCCGAAAC ATGAGCGTTT CCAGAGTCTT
    AATTTTCCTC ATACAAAAGA AATGATGAAG ATTTTTCATA AGAAATTTGG CCTGCATAAT
    TTTAGAACTA ATCAGCTAGA GGCGATCAAT GCTGCACTGC TTGGTGAAGA CTGTTTCATT
    TTAATGCCCA CTGGAGGTGG TAAAAGCCTG TGTTACCAGC TTCCTGCCTG TGTTTCTCCT
    GGGGTCACTA TTGTCATTTC CCCTTTGAGA TCACTTATAG TAGATCAAGT CCAAAAGCTG
    ACTTCCTTGG ATATCCCAGC TACATATCTG ACAGGTGATA AGACGGACTC AGAAGCTACA
    AGTATTTACC TCCAGTTATC AAAAAAAGAC CCAATTATAA AACTTCTGTA TGTTACGCCA
    GAGAAGGTCT GTGCGAGTAA CAGGCTGATC TCTACTCTGG AGAATCTGTA TGAGAGGAAG
    CTCTTGGCAC GTTTTGTCAT TGATGAAGCG CACTGTGTGA GTCAGTGGGG ACATGATTTT
    CGTCCAGATT ACAAAAGAAT GAATATTCTT CGCCAGAAGT TCCCTTCTGT TCCAGTGATG
    GCTCTCACTG CCACGGCCAA CCCCCGGGTA CAGAAGGACA TACTCACTCA GCTGAAGATT
    CTCAGGCCTC AGGTGTTTAG CATGAGCTTC AACAGACACA ATCTAAAATA CTACGTGTTA
    CCCAAAAAAC CTAAAAAGGT GGCATTTGAT TGCTTAGAAT GGATCAGAAA GCATCACCCA
    CATGACTCAG GGATAATTTA CTGCCTCTCC AGGCGAGAAT GTGACACAAT GGCTGAAACG
    TTACAGAACG ATGGCCTTGC TGCCCTGGCT TATCACGCCG GCCTCAGTGA CTCCGCCAGA
    GACGAAGTGC AGCACAAGTG GATTAATCAG GATGGCTGCC AGGTCATCTG CGCGACAATC
    GCCTTTGGAA TGGGCATCGA CAAACCGGAC GTGCGCTTTG TGATCCACGC CTCCCTCCCG
    AAGTCCGTGG AGGGTTACTA CCAGGAGTCT GGCCGAGCCG GAAGGGACGG GGAGATCTCT
    CACTGCATAC TCTTCTACAC CTACCACGAC GTGACCCGGC TGAAGAGGCT TATCCTGATG
    GAAAAAGATG GAAACCGTCA TACACGAGAG ACCCACTTCA ATAATTTGTA TAGCATGGTA
    CATTACTGTG AAAATATAAC AGAATGTAGG AGAATACAGC TTTTGGCTTA CTTTGGCGAA
    AATGAATTTA ATCCTCATTT TTGTAAGAAA TACCCAGATG TTTCATGTGA TAACTGCTAT
    AAAACAAAGG ATTTTAAGAC AAAAGATGTG ACTGAAGATG TGAAAAATAT TGTAAGATTT
    GTTCAAGAAC ACAGTTCATC TCAGGGAACA AGAAATATAA AGCATATAGG TCCTTCTGGG
    AGGTTCACTA TGAACATGCT GGTTGACATT TTCTTGGGGA GTAAGAGTGC AAAAATTCAG
    TCAGGAATAT TTGGAAAAGG ATCTACTTAT TCAAGACACA ACGCCGAAAG ACTTTTTAAA
    AAACTGATAC TTGACAGGAT TTTGGATGAA GACTTATATA TCAATGCCAA TGATCAAGCA
    ATTGCCTATG TGATGCTCGG AAGTAAAGCA CAAACCGTAC TAAATGGGCA TTTAAAGGTA
    GACTTTATGG AAACGGAAAA TTCCAGCAGT GTGAAAAAAC AAAAAGCGTT AGTGGCAAAG
    GTGTCTCAGA GGGAAGAGGT GGTGAAGAAA TGTCTTGGAG AACTTACAGA AGTCTGCAAA
    TCTCTGGGGA AAGTTTTTGG CGTCCACTAC TTTAATATTT TTAATACCGT CACTCTCAAG
    AAGCTCGCAG AATCTTTATC TTCTGATCCT GAGGTTTTGC TTCAAATTGA TGGTGTTACC
    GAAGACAAAC TGGAAAAATA TGGTGCTGAA GTGATTCCAA TATTACAGAA ATACTCTGAG
    TGGACATTGC CAGCTGAGGA CACCTCCCCA CGGAGAAGCC CCTCCAGCAG CAGAGGCACC
    AGAAAAAACA CTCCTGAGGA GTCGGATGAG GGAGCACCCA TATCTTCTCA CTACTTTGCA
    AATAAAACCA GAAACGAAAG AAAGAGGAAA AGGATGCCAG CCTCCCAAAA AGCCAAGAGG
    AGAAGAACTG GTTTCAGTGG TTCCAGGACG AAAGGGGGGT CCAACACATG CCGAAGGATA
    CCTTCTAAAA CTAAACCCTC CAACATAATC GGACCCAGTT CCACTTCTCA TAGTTCTCAA
    GCAGCATCAG GAGTCAGTAG GAAACTGGGG ATTATGGCTC CACCAAAGCC TATAAATAGA
    CCATTTCTGA AGCCTTCATA TGCATTTTCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002696629.2
    CDS828..5120
    Translation

    Target ORF information:

    RefSeq Version XM_002696583.6
    Organism Bos taurus(cattle)
    Definition Bos taurus Bloom syndrome RecQ like helicase (BLM), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002696583.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAACAACTGG AACGTCATTC GGCAAGAAAG 
    CTTAACAATA AATTGAGTCT TTCAAAACCG AAATCTTCAG GTTTCACTTT TAAAAAGAAA
    GTGCCTTCAG CCAACGATGT CTCTGTAACT AGTGTGTCAG TGGCAAAAAC ACCTGTGTTG
    AGTGATAAAG ATGTTAATAT CACTGAGGCC TTTTCCTTCA GTGAACCTTT ACCCCACACC
    ACCAATCAGC AGACGGGGAT CAATGACTTT AAAAGTGCTC CATCCGGACC GCAAACTAAG
    AGAGTTGGTT CAAAACCATT ATTGCCAAGT TTGTCGCAGG TTCCCCAGGA AGTTTCATGT
    ACGGCCCAGA ACACACCGGT TATAAAGCAA TCCCTCAATG CTACTTTCAA GAAATTAGAA
    TTTAGTTCCT CATCAGATTC TCTTATTGCC TTCAATGATT GGGATGATAT GGATGACTTC
    GATACTTCTG GAAATTCTAA AGCATTTGTG ACACCATGTA AAAACCACTT CGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGTCTAAG AGGAACTTAG TAAAAGCGCA GCTTAATAAA
    ACACATACAG GGGAGACTGA TTTGGCTCCC TTCTCCTCTG AGAGCAAGCA GGAACATTTG
    ACTGAGAAAC AGAAGGATGA CTCAGAATGG TTAAGTAGTG ATGTGATTTG CATTGATGAT
    GACCCCGATC CCGAAGAGCT GATAAATGAA GATACTCAGG AAAATCACTC GTTAAAAACT
    CATTTGGGAG CGGAAAGAGG TAATTGTGAA AAGAAGAAAA ACTTGGAAGA AACAGAATTA
    CGTTCAGTGG AGAAGTCTTC ATGTATTGAG CTTGACGAAG ATGATTATGA CATAGATTTT
    GTCCCACCTT CTCCAGAAGA AGGAATGACT TCTACCTCCT CATCCTCTTT AAAATGCTCT
    AGTATGTTGA AGGATCTTGA CACCTCTGAC AAAAAGCATA GTCTTAGCAC ATCAGAAGAC
    TTGTCAAAAC TCAAGGAAAT GGCCACACAA CAGCCTAATC AAGAAACCAG CACAAGCTGT
    GAAGCTAGAC AGACAAGTTT ACAGCAGCAG CTTATCCGTG TGATGGACCG AATCTGTAAA
    TTAGTTGATG CTGTTCCTGA TGATGAGCTG AAAGCTTTGG ACTGTGGGAA CGAGCTGCTC
    CAGCAGCGGG ACATAAGAAG GAAACTCCTA GCTGAAGCGG ATTTTGATCC CAGTGATGCC
    AGTGTTCTTA GCGCCATGTG GGGGTGTAGG CCTGATCCCC TGGAGAGCCC TGTGAAGGAC
    TTGGCCTCTG TTTCCTTACC TTACGCGTTT CGGAAGGGTG ACTCCTGTCT TGCAGGGAAT
    GCTGTGAAGG AGTTTACTCT TCCACACCTC CCCTCAAATT CCATGTCCAC CCGGGAACGT
    TTACTGACTC CTCCCCTAGG AAAGACAGGA GTCTTAGCCA CCACAAAGAG TCCCTTTGAA
    AGACCGCTAT TCAGTTCCCA TTTACAGAAG TCCTTTGTAA GTAGCAACTG GGCTGAAACA
    CCGAGGGCAG AGAAAAGAAA TGAAAGCCCT GATTTCCCAG GAAATGTTCT CACAAGCACG
    GCTGTGAAAG ATCAGAATAA ATATACCACC TCCATAAATG ACTTGGAAAG AGAGCTCCAG
    GCTGCTTGCG AGATTGACAA TTTTGACATA GATGACTTTG ACGATGATGA TGATGACTGG
    GAAAATCTGA TGCAAAATTT AGCAGCCAGC AAATCTTCCA CAGCTGCCTA CCCACCCATT
    AAGGAAGGTC GACCAGTTAA ATCTGTGTCA GAAAGACCCT CTTCCGCCAA GACAGACTGC
    CTTCCAGGGG CATCTACCAC TCAAAATAAA AACTTCTCAG AGTCAATTCC GAATTACCCT
    GACAAGATGG AGCAGAATTT AGCATCCAGA AATCCGAAAC ATGAGCGTTT CCAGAGTCTT
    AATTTTCCTC ATACAAAAGA AATGATGAAG ATTTTTCATA AGAAATTTGG CCTGCATAAT
    TTTAGAACTA ATCAGCTAGA GGCGATCAAT GCTGCACTGC TTGGTGAAGA CTGTTTCATT
    TTAATGCCCA CTGGAGGTGG TAAAAGCCTG TGTTACCAGC TTCCTGCCTG TGTTTCTCCT
    GGGGTCACTA TTGTCATTTC CCCTTTGAGA TCACTTATAG TAGATCAAGT CCAAAAGCTG
    ACTTCCTTGG ATATCCCAGC TACATATCTG ACAGGTGATA AGACGGACTC AGAAGCTACA
    AGTATTTACC TCCAGTTATC AAAAAAAGAC CCAATTATAA AACTTCTGTA TGTTACGCCA
    GAGAAGGTCT GTGCGAGTAA CAGGCTGATC TCTACTCTGG AGAATCTGTA TGAGAGGAAG
    CTCTTGGCAC GTTTTGTCAT TGATGAAGCG CACTGTGTGA GTCAGTGGGG ACATGATTTT
    CGTCCAGATT ACAAAAGAAT GAATATTCTT CGCCAGAAGT TCCCTTCTGT TCCAGTGATG
    GCTCTCACTG CCACGGCCAA CCCCCGGGTA CAGAAGGACA TACTCACTCA GCTGAAGATT
    CTCAGGCCTC AGGTGTTTAG CATGAGCTTC AACAGACACA ATCTAAAATA CTACGTGTTA
    CCCAAAAAAC CTAAAAAGGT GGCATTTGAT TGCTTAGAAT GGATCAGAAA GCATCACCCA
    CATGACTCAG GGATAATTTA CTGCCTCTCC AGGCGAGAAT GTGACACAAT GGCTGAAACG
    TTACAGAACG ATGGCCTTGC TGCCCTGGCT TATCACGCCG GCCTCAGTGA CTCCGCCAGA
    GACGAAGTGC AGCACAAGTG GATTAATCAG GATGGCTGCC AGGTCATCTG CGCGACAATC
    GCCTTTGGAA TGGGCATCGA CAAACCGGAC GTGCGCTTTG TGATCCACGC CTCCCTCCCG
    AAGTCCGTGG AGGGTTACTA CCAGGAGTCT GGCCGAGCCG GAAGGGACGG GGAGATCTCT
    CACTGCATAC TCTTCTACAC CTACCACGAC GTGACCCGGC TGAAGAGGCT TATCCTGATG
    GAAAAAGATG GAAACCGTCA TACACGAGAG ACCCACTTCA ATAATTTGTA TAGCATGGTA
    CATTACTGTG AAAATATAAC AGAATGTAGG AGAATACAGC TTTTGGCTTA CTTTGGCGAA
    AATGAATTTA ATCCTCATTT TTGTAAGAAA TACCCAGATG TTTCATGTGA TAACTGCTAT
    AAAACAAAGG ATTTTAAGAC AAAAGATGTG ACTGAAGATG TGAAAAATAT TGTAAGATTT
    GTTCAAGAAC ACAGTTCATC TCAGGGAACA AGAAATATAA AGCATATAGG TCCTTCTGGG
    AGGTTCACTA TGAACATGCT GGTTGACATT TTCTTGGGGA GTAAGAGTGC AAAAATTCAG
    TCAGGAATAT TTGGAAAAGG ATCTACTTAT TCAAGACACA ACGCCGAAAG ACTTTTTAAA
    AAACTGATAC TTGACAGGAT TTTGGATGAA GACTTATATA TCAATGCCAA TGATCAAGCA
    ATTGCCTATG TGATGCTCGG AAGTAAAGCA CAAACCGTAC TAAATGGGCA TTTAAAGGTA
    GACTTTATGG AAACGGAAAA TTCCAGCAGT GTGAAAAAAC AAAAAGCGTT AGTGGCAAAG
    GTGTCTCAGA GGGAAGAGGT GGTGAAGAAA TGTCTTGGAG AACTTACAGA AGTCTGCAAA
    TCTCTGGGGA AAGTTTTTGG CGTCCACTAC TTTAATATTT TTAATACCGT CACTCTCAAG
    AAGCTCGCAG AATCTTTATC TTCTGATCCT GAGGTTTTGC TTCAAATTGA TGGTGTTACC
    GAAGACAAAC TGGAAAAATA TGGTGCTGAA GTGATTCCAA TATTACAGAA ATACTCTGAG
    TGGACATTGC CAGCTGAGGA CACCTCCCCA CGGAGAAGCC CCTCCAGCAG CAGAGGCACC
    AGAAAAAACA CTCCTGAGGA GTCGGATGAG GGAGCACCCA TATCTTCTCA CTACTTTGCA
    AATAAAACCA GAAACGAAAG AAAGAGGAAA AGGATGCCAG CCTCCCAAAA AGCCAAGAGG
    AGAAGAACTG GTTTCAGTGG TTCCAGGACG AAAGGGGGGT CCAACACATG CCGAAGGATA
    CCTTCTAAAA CTAAACCCTC CAACATAATC GGACCCAGTT CCACTTCTCA TAGTTCTCAA
    GCAGCATCAG GAGTCAGTAG GAAACTGGGG ATTATGGCTC CACCAAAGCC TATAAATAGA
    CCATTTCTGA AGCCTTCATA TGCATTTTCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228683
    Clone ID Related Accession (Same CDS sequence) XM_010816994.3
    Accession Version XM_010816994.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product Bloom syndrome protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816994.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAACAACTGG AACGTCATTC GGCAAGAAAG 
    CTTAACAATA AATTGAGTCT TTCAAAACCG AAATCTTCAG GTTTCACTTT TAAAAAGAAA
    GTGCCTTCAG CCAACGATGT CTCTGTAACT AGTGTGTCAG TGGCAAAAAC ACCTGTGTTG
    AGTGATAAAG ATGTTAATAT CACTGAGGCC TTTTCCTTCA GTGAACCTTT ACCCCACACC
    ACCAATCAGC AGACGGGGAT CAATGACTTT AAAAGTGCTC CATCCGGACC GCAAACTAAG
    AGAGTTGGTT CAAAACCATT ATTGCCAAGT TTGTCGCAGG TTCCCCAGGA AGTTTCATGT
    ACGGCCCAGA ACACACCGGT TATAAAGCAA TCCCTCAATG CTACTTTCAA GAAATTAGAA
    TTTAGTTCCT CATCAGATTC TCTTATTGCC TTCAATGATT GGGATGATAT GGATGACTTC
    GATACTTCTG GAAATTCTAA AGCATTTGTG ACACCATGTA AAAACCACTT CGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGTCTAAG AGGAACTTAG TAAAAGCGCA GCTTAATAAA
    ACACATACAG GGGAGACTGA TTTGGCTCCC TTCTCCTCTG AGAGCAAGCA GGAACATTTG
    ACTGAGAAAC AGAAGGATGA CTCAGAATGG TTAAGTAGTG ATGTGATTTG CATTGATGAT
    GACCCCGATC CCGAAGAGCT GATAAATGAA GATACTCAGG AAAATCACTC GTTAAAAACT
    CATTTGGGAG CGGAAAGAGG TAATTGTGAA AAGAAGAAAA ACTTGGAAGA AACAGAATTA
    CGTTCAGTGG AGAAGTCTTC ATGTATTGAG CTTGACGAAG ATGATTATGA CATAGATTTT
    GTCCCACCTT CTCCAGAAGA AGGAATGACT TCTACCTCCT CATCCTCTTT AAAATGCTCT
    AGTATGTTGA AGGATCTTGA CACCTCTGAC AAAAAGCATA GTCTTAGCAC ATCAGAAGAC
    TTGTCAAAAC TCAAGGAAAT GGCCACACAA CAGCCTAATC AAGAAACCAG CACAAGCTGT
    GAAGCTAGAC AGACAAGTTT ACAGCAGCAG CTTATCCGTG TGATGGACCG AATCTGTAAA
    TTAGTTGATG CTGTTCCTGA TGATGAGCTG AAAGCTTTGG ACTGTGGGAA CGAGCTGCTC
    CAGCAGCGGG ACATAAGAAG GAAACTCCTA GCTGAAGCGG ATTTTGATCC CAGTGATGCC
    AGTGTTCTTA GCGCCATGTG GGGGTGTAGG CCTGATCCCC TGGAGAGCCC TGTGAAGGAC
    TTGGCCTCTG TTTCCTTACC TTACGCGTTT CGGAAGGGTG ACTCCTGTCT TGCAGGGAAT
    GCTGTGAAGG AGTTTACTCT TCCACACCTC CCCTCAAATT CCATGTCCAC CCGGGAACGT
    TTACTGACTC CTCCCCTAGG AAAGACAGGA GTCTTAGCCA CCACAAAGAG TCCCTTTGAA
    AGACCGCTAT TCAGTTCCCA TTTACAGAAG TCCTTTGTAA GTAGCAACTG GGCTGAAACA
    CCGAGGGCAG AGAAAAGAAA TGAAAGCCCT GATTTCCCAG GAAATGTTCT CACAAGCACG
    GCTGTGAAAG ATCAGAATAA ATATACCACC TCCATAAATG ACTTGGAAAG AGAGCTCCAG
    GCTGCTTGCG AGATTGACAA TTTTGACATA GATGACTTTG ACGATGATGA TGATGACTGG
    GAAAATCTGA TGCAAAATTT AGCAGCCAGC AAATCTTCCA CAGCTGCCTA CCCACCCATT
    AAGGAAGGTC GACCAGTTAA ATCTGTGTCA GAAAGACCCT CTTCCGCCAA GACAGACTGC
    CTTCCAGGGG CATCTACCAC TCAAAATAAA AACTTCTCAG AGTCAATTCC GAATTACCCT
    GACAAGATGG AGCAGAATTT AGCATCCAGA AATCCGAAAC ATGAGCGTTT CCAGAGTCTT
    AATTTTCCTC ATACAAAAGA AATGATGAAG ATTTTTCATA AGAAATTTGG CCTGCATAAT
    TTTAGAACTA ATCAGCTAGA GGCGATCAAT GCTGCACTGC TTGGTGAAGA CTGTTTCATT
    TTAATGCCCA CTGGAGGTGG TAAAAGCCTG TGTTACCAGC TTCCTGCCTG TGTTTCTCCT
    GGGGTCACTA TTGTCATTTC CCCTTTGAGA TCACTTATAG TAGATCAAGT CCAAAAGCTG
    ACTTCCTTGG ATATCCCAGC TACATATCTG ACAGGTGATA AGACGGACTC AGAAGCTACA
    AGTATTTACC TCCAGTTATC AAAAAAAGAC CCAATTATAA AACTTCTGTA TGTTACGCCA
    GAGAAGGTCT GTGCGAGTAA CAGGCTGATC TCTACTCTGG AGAATCTGTA TGAGAGGAAG
    CTCTTGGCAC GTTTTGTCAT TGATGAAGCG CACTGTGTGA GTCAGTGGGG ACATGATTTT
    CGTCCAGATT ACAAAAGAAT GAATATTCTT CGCCAGAAGT TCCCTTCTGT TCCAGTGATG
    GCTCTCACTG CCACGGCCAA CCCCCGGGTA CAGAAGGACA TACTCACTCA GCTGAAGATT
    CTCAGGCCTC AGGTGTTTAG CATGAGCTTC AACAGACACA ATCTAAAATA CTACGTGTTA
    CCCAAAAAAC CTAAAAAGGT GGCATTTGAT TGCTTAGAAT GGATCAGAAA GCATCACCCA
    CATGACTCAG GGATAATTTA CTGCCTCTCC AGGCGAGAAT GTGACACAAT GGCTGAAACG
    TTACAGAACG ATGGCCTTGC TGCCCTGGCT TATCACGCCG GCCTCAGTGA CTCCGCCAGA
    GACGAAGTGC AGCACAAGTG GATTAATCAG GATGGCTGCC AGGTCATCTG CGCGACAATC
    GCCTTTGGAA TGGGCATCGA CAAACCGGAC GTGCGCTTTG TGATCCACGC CTCCCTCCCG
    AAGTCCGTGG AGGGTTACTA CCAGGAGTCT GGCCGAGCCG GAAGGGACGG GGAGATCTCT
    CACTGCATAC TCTTCTACAC CTACCACGAC GTGACCCGGC TGAAGAGGCT TATCCTGAGG
    AGTAAGAGTG CAAAAATTCA GTCAGGAATA TTTGGAAAAG GATCTACTTA TTCAAGACAC
    AACGCCGAAA GACTTTTTAA AAAACTGATA CTTGACAGGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGATCAAGC AATTGCCTAT GTGATGCTCG GAAGTAAAGC ACAAACCGTA
    CTAAATGGGC ATTTAAAGGT AGACTTTATG GAAACGGAAA ATTCCAGCAG TGTGAAAAAA
    CAAAAAGCGT TAGTGGCAAA GGTGTCTCAG AGGGAAGAGG TGGTGAAGAA ATGTCTTGGA
    GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GCGTCCACTA CTTTAATATT
    TTTAATACCG TCACTCTCAA GAAGCTCGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
    CTTCAAATTG ATGGTGTTAC CGAAGACAAA CTGGAAAAAT ATGGTGCTGA AGTGATTCCA
    ATATTACAGA AATACTCTGA GTGGACATTG CCAGCTGAGG ACACCTCCCC ACGGAGAAGC
    CCCTCCAGCA GCAGAGGCAC CAGAAAAAAC ACTCCTGAGG AGTCGGATGA GGGAGCACCC
    ATATCTTCTC ACTACTTTGC AAATAAAACC AGAAACGAAA GAAAGAGGAA AAGGATGCCA
    GCCTCCCAAA AAGCCAAGAG GAGAAGAACT GGTTTCAGTG GTTCCAGGAC GAAAGGGGGG
    TCCAACACAT GCCGAAGGAT ACCTTCTAAA ACTAAACCCT CCAACATAAT CGGACCCAGT
    TCCACTTCTC ATAGTTCTCA AGCAGCATCA GGAGTCAGTA GGAAACTGGG GATTATGGCT
    CCACCAAAGC CTATAAATAG ACCATTTCTG AAGCCTTCAT ATGCATTTTC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815296.2
    CDS832..4785
    Translation

    Target ORF information:

    RefSeq Version XM_010816994.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Bloom syndrome RecQ like helicase (BLM), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816994.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAACAACTGG AACGTCATTC GGCAAGAAAG 
    CTTAACAATA AATTGAGTCT TTCAAAACCG AAATCTTCAG GTTTCACTTT TAAAAAGAAA
    GTGCCTTCAG CCAACGATGT CTCTGTAACT AGTGTGTCAG TGGCAAAAAC ACCTGTGTTG
    AGTGATAAAG ATGTTAATAT CACTGAGGCC TTTTCCTTCA GTGAACCTTT ACCCCACACC
    ACCAATCAGC AGACGGGGAT CAATGACTTT AAAAGTGCTC CATCCGGACC GCAAACTAAG
    AGAGTTGGTT CAAAACCATT ATTGCCAAGT TTGTCGCAGG TTCCCCAGGA AGTTTCATGT
    ACGGCCCAGA ACACACCGGT TATAAAGCAA TCCCTCAATG CTACTTTCAA GAAATTAGAA
    TTTAGTTCCT CATCAGATTC TCTTATTGCC TTCAATGATT GGGATGATAT GGATGACTTC
    GATACTTCTG GAAATTCTAA AGCATTTGTG ACACCATGTA AAAACCACTT CGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGTCTAAG AGGAACTTAG TAAAAGCGCA GCTTAATAAA
    ACACATACAG GGGAGACTGA TTTGGCTCCC TTCTCCTCTG AGAGCAAGCA GGAACATTTG
    ACTGAGAAAC AGAAGGATGA CTCAGAATGG TTAAGTAGTG ATGTGATTTG CATTGATGAT
    GACCCCGATC CCGAAGAGCT GATAAATGAA GATACTCAGG AAAATCACTC GTTAAAAACT
    CATTTGGGAG CGGAAAGAGG TAATTGTGAA AAGAAGAAAA ACTTGGAAGA AACAGAATTA
    CGTTCAGTGG AGAAGTCTTC ATGTATTGAG CTTGACGAAG ATGATTATGA CATAGATTTT
    GTCCCACCTT CTCCAGAAGA AGGAATGACT TCTACCTCCT CATCCTCTTT AAAATGCTCT
    AGTATGTTGA AGGATCTTGA CACCTCTGAC AAAAAGCATA GTCTTAGCAC ATCAGAAGAC
    TTGTCAAAAC TCAAGGAAAT GGCCACACAA CAGCCTAATC AAGAAACCAG CACAAGCTGT
    GAAGCTAGAC AGACAAGTTT ACAGCAGCAG CTTATCCGTG TGATGGACCG AATCTGTAAA
    TTAGTTGATG CTGTTCCTGA TGATGAGCTG AAAGCTTTGG ACTGTGGGAA CGAGCTGCTC
    CAGCAGCGGG ACATAAGAAG GAAACTCCTA GCTGAAGCGG ATTTTGATCC CAGTGATGCC
    AGTGTTCTTA GCGCCATGTG GGGGTGTAGG CCTGATCCCC TGGAGAGCCC TGTGAAGGAC
    TTGGCCTCTG TTTCCTTACC TTACGCGTTT CGGAAGGGTG ACTCCTGTCT TGCAGGGAAT
    GCTGTGAAGG AGTTTACTCT TCCACACCTC CCCTCAAATT CCATGTCCAC CCGGGAACGT
    TTACTGACTC CTCCCCTAGG AAAGACAGGA GTCTTAGCCA CCACAAAGAG TCCCTTTGAA
    AGACCGCTAT TCAGTTCCCA TTTACAGAAG TCCTTTGTAA GTAGCAACTG GGCTGAAACA
    CCGAGGGCAG AGAAAAGAAA TGAAAGCCCT GATTTCCCAG GAAATGTTCT CACAAGCACG
    GCTGTGAAAG ATCAGAATAA ATATACCACC TCCATAAATG ACTTGGAAAG AGAGCTCCAG
    GCTGCTTGCG AGATTGACAA TTTTGACATA GATGACTTTG ACGATGATGA TGATGACTGG
    GAAAATCTGA TGCAAAATTT AGCAGCCAGC AAATCTTCCA CAGCTGCCTA CCCACCCATT
    AAGGAAGGTC GACCAGTTAA ATCTGTGTCA GAAAGACCCT CTTCCGCCAA GACAGACTGC
    CTTCCAGGGG CATCTACCAC TCAAAATAAA AACTTCTCAG AGTCAATTCC GAATTACCCT
    GACAAGATGG AGCAGAATTT AGCATCCAGA AATCCGAAAC ATGAGCGTTT CCAGAGTCTT
    AATTTTCCTC ATACAAAAGA AATGATGAAG ATTTTTCATA AGAAATTTGG CCTGCATAAT
    TTTAGAACTA ATCAGCTAGA GGCGATCAAT GCTGCACTGC TTGGTGAAGA CTGTTTCATT
    TTAATGCCCA CTGGAGGTGG TAAAAGCCTG TGTTACCAGC TTCCTGCCTG TGTTTCTCCT
    GGGGTCACTA TTGTCATTTC CCCTTTGAGA TCACTTATAG TAGATCAAGT CCAAAAGCTG
    ACTTCCTTGG ATATCCCAGC TACATATCTG ACAGGTGATA AGACGGACTC AGAAGCTACA
    AGTATTTACC TCCAGTTATC AAAAAAAGAC CCAATTATAA AACTTCTGTA TGTTACGCCA
    GAGAAGGTCT GTGCGAGTAA CAGGCTGATC TCTACTCTGG AGAATCTGTA TGAGAGGAAG
    CTCTTGGCAC GTTTTGTCAT TGATGAAGCG CACTGTGTGA GTCAGTGGGG ACATGATTTT
    CGTCCAGATT ACAAAAGAAT GAATATTCTT CGCCAGAAGT TCCCTTCTGT TCCAGTGATG
    GCTCTCACTG CCACGGCCAA CCCCCGGGTA CAGAAGGACA TACTCACTCA GCTGAAGATT
    CTCAGGCCTC AGGTGTTTAG CATGAGCTTC AACAGACACA ATCTAAAATA CTACGTGTTA
    CCCAAAAAAC CTAAAAAGGT GGCATTTGAT TGCTTAGAAT GGATCAGAAA GCATCACCCA
    CATGACTCAG GGATAATTTA CTGCCTCTCC AGGCGAGAAT GTGACACAAT GGCTGAAACG
    TTACAGAACG ATGGCCTTGC TGCCCTGGCT TATCACGCCG GCCTCAGTGA CTCCGCCAGA
    GACGAAGTGC AGCACAAGTG GATTAATCAG GATGGCTGCC AGGTCATCTG CGCGACAATC
    GCCTTTGGAA TGGGCATCGA CAAACCGGAC GTGCGCTTTG TGATCCACGC CTCCCTCCCG
    AAGTCCGTGG AGGGTTACTA CCAGGAGTCT GGCCGAGCCG GAAGGGACGG GGAGATCTCT
    CACTGCATAC TCTTCTACAC CTACCACGAC GTGACCCGGC TGAAGAGGCT TATCCTGAGG
    AGTAAGAGTG CAAAAATTCA GTCAGGAATA TTTGGAAAAG GATCTACTTA TTCAAGACAC
    AACGCCGAAA GACTTTTTAA AAAACTGATA CTTGACAGGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGATCAAGC AATTGCCTAT GTGATGCTCG GAAGTAAAGC ACAAACCGTA
    CTAAATGGGC ATTTAAAGGT AGACTTTATG GAAACGGAAA ATTCCAGCAG TGTGAAAAAA
    CAAAAAGCGT TAGTGGCAAA GGTGTCTCAG AGGGAAGAGG TGGTGAAGAA ATGTCTTGGA
    GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GCGTCCACTA CTTTAATATT
    TTTAATACCG TCACTCTCAA GAAGCTCGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
    CTTCAAATTG ATGGTGTTAC CGAAGACAAA CTGGAAAAAT ATGGTGCTGA AGTGATTCCA
    ATATTACAGA AATACTCTGA GTGGACATTG CCAGCTGAGG ACACCTCCCC ACGGAGAAGC
    CCCTCCAGCA GCAGAGGCAC CAGAAAAAAC ACTCCTGAGG AGTCGGATGA GGGAGCACCC
    ATATCTTCTC ACTACTTTGC AAATAAAACC AGAAACGAAA GAAAGAGGAA AAGGATGCCA
    GCCTCCCAAA AAGCCAAGAG GAGAAGAACT GGTTTCAGTG GTTCCAGGAC GAAAGGGGGG
    TCCAACACAT GCCGAAGGAT ACCTTCTAAA ACTAAACCCT CCAACATAAT CGGACCCAGT
    TCCACTTCTC ATAGTTCTCA AGCAGCATCA GGAGTCAGTA GGAAACTGGG GATTATGGCT
    CCACCAAAGC CTATAAATAG ACCATTTCTG AAGCCTTCAT ATGCATTTTC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52650
    Clone ID Related Accession (Same CDS sequence) XM_010816994.2 , XM_010824828.2
    Accession Version XM_010816994.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product Bloom syndrome protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAACAACTGG AACGTCATTC GGCAAGAAAG 
    CTTAACAATA AATTGGGTCT TTCAAAACCG AAATCTTCAG GTTTCACTTT TAAAAAGAAA
    GTGCCTTCAG CCAACAATGT CTCTGTAACT AGTGTGTCAG TGGCAAAAAC ACCTGTGTTG
    AGTGATAAAG ATGTTAATAT CACTGAGGCC TTTTCCTTCA GTGAACCTTT ACCCCACACC
    ACCAATCAGC AGACGGGGAT CAATGACTTT AAAAGTGCTC CATCCGGACC GCAAACTAAG
    AGAGTTGGTT CAAAACCATT ATTGCCAAGT TTGTCGCAGG TTCCCCAGGA AGTTTCATGT
    ACGGCCCAGA ACACACCGGT TATAAAGCAA TCCCTCAATG CTACTTTCAA GAAATTAGAA
    TTTAGTTCCT CATCAGATTC TCTTATTGCC TTCAATGATT GGGATGATAT GGATGACTTC
    GATACTTCTG GAAATTCTAA AGCATTTGTG ACACCATGTA AAAACCACTT CGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGTCTAAG AGGAACTTAG TAAAAGCGCA GCTTAATAAA
    ACACATACAG GGGAGACTGA TTTGGCTCCC TCCTCCTCTG AGAGCAAGCA GGAACATTTG
    ACTGAGAAAC AGAAGGATGA CTCAGAATGG TTAAGTAGTG ATGTGATTTG CATTGATGAT
    GACCCCGATC CCGAAGAGCT GATAAATGAA GATACTCAGG AAAATCACTC GTTAAAAACT
    CATTTGGGAG CGGAAAGAGG TAATTGTGAA AAGAAGAAAA ACTTGGAAGA AACAGAATTA
    CGTTCAGTGG AGAAGTCTTC ATGTATTGAG CTTGACGAAG ATGATTATGA CATAGATTTT
    GTCCCACCTT CTCCAGAAGA AGGAATGACT TCTACCTCCT CATCCTCTTT AAAATGCTCT
    AGTATGTTGA AGGATCTTGA CACCTCTGAC AAAAAGCATA GTCTTAGCAC ATCAGAAGAC
    TTGTCAAAAC TCAAGGAAAT GGCCACACAA CAGCCTAATC AAGAAACCAG CACAAGCTGT
    GAAGCTAGAC AGACAAGTTT ACAGCAGCAG CTTATCCGTG TGATGGACCG AATCTGTAAA
    TTAGTTGATG CTGTTCCTGA TGATGAGCTG AAAGCTTTGG ACTGTGGGAA CGAGCTGCTC
    CAGCAGCGGG ACATAAGAAG GAAACTCCTA GCTGAAGCGG ATTTTGATCC CAGTGATGCC
    AGTGTTCTTA GCGCCATGTG GGGGTGTAGG CCTGATCCCC TGGAGAGCCC TGTGAAGGAC
    TTGGCCTCTG TTTCCTTACC TTACGCGTTT CGGAAGGGTG ACTCCTGTCT TGCAGGGAAT
    GCTGTGAAGG AGTTTACTCT TCCACACCTC CCCTCAAATT CCATGTCCAC CCGGGAACGT
    TTACTGACTC CTCCCCTAGG AAAGACAGGA GTCTTAGCCA CCACAAAGAG TCCCTTTGAA
    AGACCGCTAT TCAGTTCCCA TTTACAGAAG TCCTTTGTAA GTAGCAACTG GGCTGAAACA
    CCGAGGGCAG AGAAAAGAAA TGAAAGCCCT GATTTCCCAG GAAATGTTCT CACAAGCACG
    GCTGTGAAAG ATCAGAATAA ATATACCACC TCCATAAATG ACTTGGAAAG AGAGCTCCAG
    GCTGCTTGCG AGATTGACAA TTTTGACATA GATGACTTTG ACGATGATGA TGATGACTGG
    GAAAATCTGA TGCAAAATTT AGCAGCCAGC AAATCTTCCA CAGCTGCCTA CCCACCCATT
    AAGGAAGGTC GACCAGTTAA ATCTGTGTCA GAAAGACCCT CTTCCGCCAA GACAGACTGC
    CTTCCAGGGG CATCTACCAC TCAAAATAAA AACTTCTCAG AGTCAATTCC GAATTACCCT
    GACAAGATGG AGCAGAATTT AGCATCCAGA AATCCGAAAC ATGAGCGTTT CCAGAGTCTT
    AATTTTCCTC ATACAAAAGA AATGATGAAG ATTTTTCATA AGAAATTTGG CCTGCATAAT
    TTTAGAACTA ATCAGCTAGA GGCGATCAAT GCTGCACTGC TTGGTGAAGA CTGTTTCATT
    TTAATGCCCA CTGGAGGTGG TAAAAGCCTG TGTTACCAGC TTCCTGCCTG TGTTTCTCCT
    GGGGTCACTA TTGTCATTTC CCCTTTGAGA TCACTTATAG TAGATCAAGT CCAAAAGCTG
    ACTTCCTTGG ATATCCCAGC TACATATCTG ACAGGTGATA AGACGGACTC AGAAGCTACA
    AGTATTTACC TCCAGTTATC AAAAAAAGAC CCAATTATAA AACTTCTGTA TGTTACGCCA
    GAGAAGGTCT GTGCGAGTAA CAGGCTGATC TCTACTCTGG AGAATCTGTA TGAGAGGAAG
    CTCTTGGCAC GTTTTGTCAT TGATGAAGCG CACTGTGTGA GTCAGTGGGG ACATGATTTT
    CGTCCAGATT ACAAAAGAAT GAATATTCTT CGCCAGAAGT TCCCTTCTGT TCCAGTGATG
    GCTCTCACTG CCACGGCCAA CCCCCGGGTA CAGAAGGACA TACTCACTCA GCTGAAGATT
    CTCAGGCCTC AGGTGTTTAG CATGAGCTTC AACAGACACA ATCTAAAATA CTACGTGTTA
    CCCAAAAAAC CTAAAAAGGT GGCATTTGAT TGCTTAGAAT GGATCAGAAA GCATCACCCA
    CATGACTCAG GGATAATTTA CTGCCTCTCC AGGCGAGAAT GTGACACAAT GGCTGAAACG
    TTACAGAACG ATGGCCTTGC TGCCCTGGCT TATCACGCCG GCCTCAGTGA CTCCGCCAGA
    GACGAAGTGC AGCACAAGTG GATTAATCAG GATGGCTGCC AGGTCATCTG CGCGACAATC
    GCCTTTGGAA TGGGCATCGA CAAACCGGAC GTGCGCTTTG TGATCCACGC CTCCCTCCCG
    AAGTCCGTGG AGGGTTACTA CCAGGAGTCT GGCCGAGCCG GAAGGGACGG GGAGATCTCT
    CACTGCATAC TCTTCTACAC CTACCACGAC GTGACCCGGC TGAAGAGGCT TATCCTGAGG
    AGTAAGAGTG CAAAAATTCA GTCAGGAATA TTTGGAAAAG GATCTACTTA TTCAAGACAC
    AACGCCGAAA GACTTTTTAA AAAACTGATA CTTGACAGGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGATCAAGC AATTGCCTAT GTGATGCTCG GAAGTAAAGC ACAAACCGTA
    CTAAATGGGC ATTTAAAGGT AGACTTTATG GAAACGGAAA ATTCCAGCAG TGTGAAAAAA
    CAAAAAGCGT TAGTGGCAAA GGTGTCTCAG AGGGAAGAGG TGGTGAAGAA ATGTCTTGGA
    GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GCGTCCACTA CTTTAATATT
    TTTAATACCG TCACTCTCAA GAAGCTCGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
    CTTCAAATTG ATGGTGTTAC CGAAGACAAA CTGGAAAAAT ATGGTGCTGA AGTGATTCCA
    ATATTACAGA AATACTCTGA GTGGACATTG CCAGCTGAGG ACACCTCCCC ACGGAGAAGC
    CCCTCCAGCA GCAGAGGCAC CAGAAAAAAC ACTCCTGAGG AGTCGGATGA GGGAGCACCC
    ATATCTTCTC ACTACTTTGC AAATAAAACC AGAAACGAAA GAAAGAGGAA AAGGATGCCA
    GCCTCCCAAA AAGCCAAGAG GAGAAGAACT GGTTTCAGTG GTTCCAGGAC GAAAGGGGGG
    TCCAACACAT GCCGAAGGAT ACCTTCTAAA ACTAAACCCT CCAACATAAT CGGACCCAGT
    TCCACTTCTC ATAGTTCTCA AGCAGCATCA GGAGTCAGTA GGAAACTGGG GATTATGGCT
    CCACCAAAGC CTATAAATAG ACCATTTCTG AAGCCTTCAT ATGCATTTTC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815296.1
    CDS698..4651
    Translation

    Target ORF information:

    RefSeq Version XM_010816994.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Bloom syndrome, RecQ helicase-like (BLM), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816994.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAACAACTGG AACGTCATTC GGCAAGAAAG 
    CTTAACAATA AATTGGGTCT TTCAAAACCG AAATCTTCAG GTTTCACTTT TAAAAAGAAA
    GTGCCTTCAG CCAACAATGT CTCTGTAACT AGTGTGTCAG TGGCAAAAAC ACCTGTGTTG
    AGTGATAAAG ATGTTAATAT CACTGAGGCC TTTTCCTTCA GTGAACCTTT ACCCCACACC
    ACCAATCAGC AGACGGGGAT CAATGACTTT AAAAGTGCTC CATCCGGACC GCAAACTAAG
    AGAGTTGGTT CAAAACCATT ATTGCCAAGT TTGTCGCAGG TTCCCCAGGA AGTTTCATGT
    ACGGCCCAGA ACACACCGGT TATAAAGCAA TCCCTCAATG CTACTTTCAA GAAATTAGAA
    TTTAGTTCCT CATCAGATTC TCTTATTGCC TTCAATGATT GGGATGATAT GGATGACTTC
    GATACTTCTG GAAATTCTAA AGCATTTGTG ACACCATGTA AAAACCACTT CGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGTCTAAG AGGAACTTAG TAAAAGCGCA GCTTAATAAA
    ACACATACAG GGGAGACTGA TTTGGCTCCC TCCTCCTCTG AGAGCAAGCA GGAACATTTG
    ACTGAGAAAC AGAAGGATGA CTCAGAATGG TTAAGTAGTG ATGTGATTTG CATTGATGAT
    GACCCCGATC CCGAAGAGCT GATAAATGAA GATACTCAGG AAAATCACTC GTTAAAAACT
    CATTTGGGAG CGGAAAGAGG TAATTGTGAA AAGAAGAAAA ACTTGGAAGA AACAGAATTA
    CGTTCAGTGG AGAAGTCTTC ATGTATTGAG CTTGACGAAG ATGATTATGA CATAGATTTT
    GTCCCACCTT CTCCAGAAGA AGGAATGACT TCTACCTCCT CATCCTCTTT AAAATGCTCT
    AGTATGTTGA AGGATCTTGA CACCTCTGAC AAAAAGCATA GTCTTAGCAC ATCAGAAGAC
    TTGTCAAAAC TCAAGGAAAT GGCCACACAA CAGCCTAATC AAGAAACCAG CACAAGCTGT
    GAAGCTAGAC AGACAAGTTT ACAGCAGCAG CTTATCCGTG TGATGGACCG AATCTGTAAA
    TTAGTTGATG CTGTTCCTGA TGATGAGCTG AAAGCTTTGG ACTGTGGGAA CGAGCTGCTC
    CAGCAGCGGG ACATAAGAAG GAAACTCCTA GCTGAAGCGG ATTTTGATCC CAGTGATGCC
    AGTGTTCTTA GCGCCATGTG GGGGTGTAGG CCTGATCCCC TGGAGAGCCC TGTGAAGGAC
    TTGGCCTCTG TTTCCTTACC TTACGCGTTT CGGAAGGGTG ACTCCTGTCT TGCAGGGAAT
    GCTGTGAAGG AGTTTACTCT TCCACACCTC CCCTCAAATT CCATGTCCAC CCGGGAACGT
    TTACTGACTC CTCCCCTAGG AAAGACAGGA GTCTTAGCCA CCACAAAGAG TCCCTTTGAA
    AGACCGCTAT TCAGTTCCCA TTTACAGAAG TCCTTTGTAA GTAGCAACTG GGCTGAAACA
    CCGAGGGCAG AGAAAAGAAA TGAAAGCCCT GATTTCCCAG GAAATGTTCT CACAAGCACG
    GCTGTGAAAG ATCAGAATAA ATATACCACC TCCATAAATG ACTTGGAAAG AGAGCTCCAG
    GCTGCTTGCG AGATTGACAA TTTTGACATA GATGACTTTG ACGATGATGA TGATGACTGG
    GAAAATCTGA TGCAAAATTT AGCAGCCAGC AAATCTTCCA CAGCTGCCTA CCCACCCATT
    AAGGAAGGTC GACCAGTTAA ATCTGTGTCA GAAAGACCCT CTTCCGCCAA GACAGACTGC
    CTTCCAGGGG CATCTACCAC TCAAAATAAA AACTTCTCAG AGTCAATTCC GAATTACCCT
    GACAAGATGG AGCAGAATTT AGCATCCAGA AATCCGAAAC ATGAGCGTTT CCAGAGTCTT
    AATTTTCCTC ATACAAAAGA AATGATGAAG ATTTTTCATA AGAAATTTGG CCTGCATAAT
    TTTAGAACTA ATCAGCTAGA GGCGATCAAT GCTGCACTGC TTGGTGAAGA CTGTTTCATT
    TTAATGCCCA CTGGAGGTGG TAAAAGCCTG TGTTACCAGC TTCCTGCCTG TGTTTCTCCT
    GGGGTCACTA TTGTCATTTC CCCTTTGAGA TCACTTATAG TAGATCAAGT CCAAAAGCTG
    ACTTCCTTGG ATATCCCAGC TACATATCTG ACAGGTGATA AGACGGACTC AGAAGCTACA
    AGTATTTACC TCCAGTTATC AAAAAAAGAC CCAATTATAA AACTTCTGTA TGTTACGCCA
    GAGAAGGTCT GTGCGAGTAA CAGGCTGATC TCTACTCTGG AGAATCTGTA TGAGAGGAAG
    CTCTTGGCAC GTTTTGTCAT TGATGAAGCG CACTGTGTGA GTCAGTGGGG ACATGATTTT
    CGTCCAGATT ACAAAAGAAT GAATATTCTT CGCCAGAAGT TCCCTTCTGT TCCAGTGATG
    GCTCTCACTG CCACGGCCAA CCCCCGGGTA CAGAAGGACA TACTCACTCA GCTGAAGATT
    CTCAGGCCTC AGGTGTTTAG CATGAGCTTC AACAGACACA ATCTAAAATA CTACGTGTTA
    CCCAAAAAAC CTAAAAAGGT GGCATTTGAT TGCTTAGAAT GGATCAGAAA GCATCACCCA
    CATGACTCAG GGATAATTTA CTGCCTCTCC AGGCGAGAAT GTGACACAAT GGCTGAAACG
    TTACAGAACG ATGGCCTTGC TGCCCTGGCT TATCACGCCG GCCTCAGTGA CTCCGCCAGA
    GACGAAGTGC AGCACAAGTG GATTAATCAG GATGGCTGCC AGGTCATCTG CGCGACAATC
    GCCTTTGGAA TGGGCATCGA CAAACCGGAC GTGCGCTTTG TGATCCACGC CTCCCTCCCG
    AAGTCCGTGG AGGGTTACTA CCAGGAGTCT GGCCGAGCCG GAAGGGACGG GGAGATCTCT
    CACTGCATAC TCTTCTACAC CTACCACGAC GTGACCCGGC TGAAGAGGCT TATCCTGAGG
    AGTAAGAGTG CAAAAATTCA GTCAGGAATA TTTGGAAAAG GATCTACTTA TTCAAGACAC
    AACGCCGAAA GACTTTTTAA AAAACTGATA CTTGACAGGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGATCAAGC AATTGCCTAT GTGATGCTCG GAAGTAAAGC ACAAACCGTA
    CTAAATGGGC ATTTAAAGGT AGACTTTATG GAAACGGAAA ATTCCAGCAG TGTGAAAAAA
    CAAAAAGCGT TAGTGGCAAA GGTGTCTCAG AGGGAAGAGG TGGTGAAGAA ATGTCTTGGA
    GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GCGTCCACTA CTTTAATATT
    TTTAATACCG TCACTCTCAA GAAGCTCGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
    CTTCAAATTG ATGGTGTTAC CGAAGACAAA CTGGAAAAAT ATGGTGCTGA AGTGATTCCA
    ATATTACAGA AATACTCTGA GTGGACATTG CCAGCTGAGG ACACCTCCCC ACGGAGAAGC
    CCCTCCAGCA GCAGAGGCAC CAGAAAAAAC ACTCCTGAGG AGTCGGATGA GGGAGCACCC
    ATATCTTCTC ACTACTTTGC AAATAAAACC AGAAACGAAA GAAAGAGGAA AAGGATGCCA
    GCCTCCCAAA AAGCCAAGAG GAGAAGAACT GGTTTCAGTG GTTCCAGGAC GAAAGGGGGG
    TCCAACACAT GCCGAAGGAT ACCTTCTAAA ACTAAACCCT CCAACATAAT CGGACCCAGT
    TCCACTTCTC ATAGTTCTCA AGCAGCATCA GGAGTCAGTA GGAAACTGGG GATTATGGCT
    CCACCAAAGC CTATAAATAG ACCATTTCTG AAGCCTTCAT ATGCATTTTC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52650
    Clone ID Related Accession (Same CDS sequence) XM_010816994.2 , XM_010824828.2
    Accession Version XM_010824828.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product Bloom syndrome protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646530.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824828.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAACAACTGG AACGTCATTC GGCAAGAAAG 
    CTTAACAATA AATTGGGTCT TTCAAAACCG AAATCTTCAG GTTTCACTTT TAAAAAGAAA
    GTGCCTTCAG CCAACAATGT CTCTGTAACT AGTGTGTCAG TGGCAAAAAC ACCTGTGTTG
    AGTGATAAAG ATGTTAATAT CACTGAGGCC TTTTCCTTCA GTGAACCTTT ACCCCACACC
    ACCAATCAGC AGACGGGGAT CAATGACTTT AAAAGTGCTC CATCCGGACC GCAAACTAAG
    AGAGTTGGTT CAAAACCATT ATTGCCAAGT TTGTCGCAGG TTCCCCAGGA AGTTTCATGT
    ACGGCCCAGA ACACACCGGT TATAAAGCAA TCCCTCAATG CTACTTTCAA GAAATTAGAA
    TTTAGTTCCT CATCAGATTC TCTTATTGCC TTCAATGATT GGGATGATAT GGATGACTTC
    GATACTTCTG GAAATTCTAA AGCATTTGTG ACACCATGTA AAAACCACTT CGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGTCTAAG AGGAACTTAG TAAAAGCGCA GCTTAATAAA
    ACACATACAG GGGAGACTGA TTTGGCTCCC TCCTCCTCTG AGAGCAAGCA GGAACATTTG
    ACTGAGAAAC AGAAGGATGA CTCAGAATGG TTAAGTAGTG ATGTGATTTG CATTGATGAT
    GACCCCGATC CCGAAGAGCT GATAAATGAA GATACTCAGG AAAATCACTC GTTAAAAACT
    CATTTGGGAG CGGAAAGAGG TAATTGTGAA AAGAAGAAAA ACTTGGAAGA AACAGAATTA
    CGTTCAGTGG AGAAGTCTTC ATGTATTGAG CTTGACGAAG ATGATTATGA CATAGATTTT
    GTCCCACCTT CTCCAGAAGA AGGAATGACT TCTACCTCCT CATCCTCTTT AAAATGCTCT
    AGTATGTTGA AGGATCTTGA CACCTCTGAC AAAAAGCATA GTCTTAGCAC ATCAGAAGAC
    TTGTCAAAAC TCAAGGAAAT GGCCACACAA CAGCCTAATC AAGAAACCAG CACAAGCTGT
    GAAGCTAGAC AGACAAGTTT ACAGCAGCAG CTTATCCGTG TGATGGACCG AATCTGTAAA
    TTAGTTGATG CTGTTCCTGA TGATGAGCTG AAAGCTTTGG ACTGTGGGAA CGAGCTGCTC
    CAGCAGCGGG ACATAAGAAG GAAACTCCTA GCTGAAGCGG ATTTTGATCC CAGTGATGCC
    AGTGTTCTTA GCGCCATGTG GGGGTGTAGG CCTGATCCCC TGGAGAGCCC TGTGAAGGAC
    TTGGCCTCTG TTTCCTTACC TTACGCGTTT CGGAAGGGTG ACTCCTGTCT TGCAGGGAAT
    GCTGTGAAGG AGTTTACTCT TCCACACCTC CCCTCAAATT CCATGTCCAC CCGGGAACGT
    TTACTGACTC CTCCCCTAGG AAAGACAGGA GTCTTAGCCA CCACAAAGAG TCCCTTTGAA
    AGACCGCTAT TCAGTTCCCA TTTACAGAAG TCCTTTGTAA GTAGCAACTG GGCTGAAACA
    CCGAGGGCAG AGAAAAGAAA TGAAAGCCCT GATTTCCCAG GAAATGTTCT CACAAGCACG
    GCTGTGAAAG ATCAGAATAA ATATACCACC TCCATAAATG ACTTGGAAAG AGAGCTCCAG
    GCTGCTTGCG AGATTGACAA TTTTGACATA GATGACTTTG ACGATGATGA TGATGACTGG
    GAAAATCTGA TGCAAAATTT AGCAGCCAGC AAATCTTCCA CAGCTGCCTA CCCACCCATT
    AAGGAAGGTC GACCAGTTAA ATCTGTGTCA GAAAGACCCT CTTCCGCCAA GACAGACTGC
    CTTCCAGGGG CATCTACCAC TCAAAATAAA AACTTCTCAG AGTCAATTCC GAATTACCCT
    GACAAGATGG AGCAGAATTT AGCATCCAGA AATCCGAAAC ATGAGCGTTT CCAGAGTCTT
    AATTTTCCTC ATACAAAAGA AATGATGAAG ATTTTTCATA AGAAATTTGG CCTGCATAAT
    TTTAGAACTA ATCAGCTAGA GGCGATCAAT GCTGCACTGC TTGGTGAAGA CTGTTTCATT
    TTAATGCCCA CTGGAGGTGG TAAAAGCCTG TGTTACCAGC TTCCTGCCTG TGTTTCTCCT
    GGGGTCACTA TTGTCATTTC CCCTTTGAGA TCACTTATAG TAGATCAAGT CCAAAAGCTG
    ACTTCCTTGG ATATCCCAGC TACATATCTG ACAGGTGATA AGACGGACTC AGAAGCTACA
    AGTATTTACC TCCAGTTATC AAAAAAAGAC CCAATTATAA AACTTCTGTA TGTTACGCCA
    GAGAAGGTCT GTGCGAGTAA CAGGCTGATC TCTACTCTGG AGAATCTGTA TGAGAGGAAG
    CTCTTGGCAC GTTTTGTCAT TGATGAAGCG CACTGTGTGA GTCAGTGGGG ACATGATTTT
    CGTCCAGATT ACAAAAGAAT GAATATTCTT CGCCAGAAGT TCCCTTCTGT TCCAGTGATG
    GCTCTCACTG CCACGGCCAA CCCCCGGGTA CAGAAGGACA TACTCACTCA GCTGAAGATT
    CTCAGGCCTC AGGTGTTTAG CATGAGCTTC AACAGACACA ATCTAAAATA CTACGTGTTA
    CCCAAAAAAC CTAAAAAGGT GGCATTTGAT TGCTTAGAAT GGATCAGAAA GCATCACCCA
    CATGACTCAG GGATAATTTA CTGCCTCTCC AGGCGAGAAT GTGACACAAT GGCTGAAACG
    TTACAGAACG ATGGCCTTGC TGCCCTGGCT TATCACGCCG GCCTCAGTGA CTCCGCCAGA
    GACGAAGTGC AGCACAAGTG GATTAATCAG GATGGCTGCC AGGTCATCTG CGCGACAATC
    GCCTTTGGAA TGGGCATCGA CAAACCGGAC GTGCGCTTTG TGATCCACGC CTCCCTCCCG
    AAGTCCGTGG AGGGTTACTA CCAGGAGTCT GGCCGAGCCG GAAGGGACGG GGAGATCTCT
    CACTGCATAC TCTTCTACAC CTACCACGAC GTGACCCGGC TGAAGAGGCT TATCCTGAGG
    AGTAAGAGTG CAAAAATTCA GTCAGGAATA TTTGGAAAAG GATCTACTTA TTCAAGACAC
    AACGCCGAAA GACTTTTTAA AAAACTGATA CTTGACAGGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGATCAAGC AATTGCCTAT GTGATGCTCG GAAGTAAAGC ACAAACCGTA
    CTAAATGGGC ATTTAAAGGT AGACTTTATG GAAACGGAAA ATTCCAGCAG TGTGAAAAAA
    CAAAAAGCGT TAGTGGCAAA GGTGTCTCAG AGGGAAGAGG TGGTGAAGAA ATGTCTTGGA
    GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GCGTCCACTA CTTTAATATT
    TTTAATACCG TCACTCTCAA GAAGCTCGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
    CTTCAAATTG ATGGTGTTAC CGAAGACAAA CTGGAAAAAT ATGGTGCTGA AGTGATTCCA
    ATATTACAGA AATACTCTGA GTGGACATTG CCAGCTGAGG ACACCTCCCC ACGGAGAAGC
    CCCTCCAGCA GCAGAGGCAC CAGAAAAAAC ACTCCTGAGG AGTCGGATGA GGGAGCACCC
    ATATCTTCTC ACTACTTTGC AAATAAAACC AGAAACGAAA GAAAGAGGAA AAGGATGCCA
    GCCTCCCAAA AAGCCAAGAG GAGAAGAACT GGTTTCAGTG GTTCCAGGAC GAAAGGGGGG
    TCCAACACAT GCCGAAGGAT ACCTTCTAAA ACTAAACCCT CCAACATAAT CGGACCCAGT
    TCCACTTCTC ATAGTTCTCA AGCAGCATCA GGAGTCAGTA GGAAACTGGG GATTATGGCT
    CCACCAAAGC CTATAAATAG ACCATTTCTG AAGCCTTCAT ATGCATTTTC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010823130.2
    CDS698..4651
    Translation

    Target ORF information:

    RefSeq Version XM_010824828.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Bloom syndrome, RecQ helicase-like (BLM), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824828.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAACAACTGG AACGTCATTC GGCAAGAAAG 
    CTTAACAATA AATTGGGTCT TTCAAAACCG AAATCTTCAG GTTTCACTTT TAAAAAGAAA
    GTGCCTTCAG CCAACAATGT CTCTGTAACT AGTGTGTCAG TGGCAAAAAC ACCTGTGTTG
    AGTGATAAAG ATGTTAATAT CACTGAGGCC TTTTCCTTCA GTGAACCTTT ACCCCACACC
    ACCAATCAGC AGACGGGGAT CAATGACTTT AAAAGTGCTC CATCCGGACC GCAAACTAAG
    AGAGTTGGTT CAAAACCATT ATTGCCAAGT TTGTCGCAGG TTCCCCAGGA AGTTTCATGT
    ACGGCCCAGA ACACACCGGT TATAAAGCAA TCCCTCAATG CTACTTTCAA GAAATTAGAA
    TTTAGTTCCT CATCAGATTC TCTTATTGCC TTCAATGATT GGGATGATAT GGATGACTTC
    GATACTTCTG GAAATTCTAA AGCATTTGTG ACACCATGTA AAAACCACTT CGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGTCTAAG AGGAACTTAG TAAAAGCGCA GCTTAATAAA
    ACACATACAG GGGAGACTGA TTTGGCTCCC TCCTCCTCTG AGAGCAAGCA GGAACATTTG
    ACTGAGAAAC AGAAGGATGA CTCAGAATGG TTAAGTAGTG ATGTGATTTG CATTGATGAT
    GACCCCGATC CCGAAGAGCT GATAAATGAA GATACTCAGG AAAATCACTC GTTAAAAACT
    CATTTGGGAG CGGAAAGAGG TAATTGTGAA AAGAAGAAAA ACTTGGAAGA AACAGAATTA
    CGTTCAGTGG AGAAGTCTTC ATGTATTGAG CTTGACGAAG ATGATTATGA CATAGATTTT
    GTCCCACCTT CTCCAGAAGA AGGAATGACT TCTACCTCCT CATCCTCTTT AAAATGCTCT
    AGTATGTTGA AGGATCTTGA CACCTCTGAC AAAAAGCATA GTCTTAGCAC ATCAGAAGAC
    TTGTCAAAAC TCAAGGAAAT GGCCACACAA CAGCCTAATC AAGAAACCAG CACAAGCTGT
    GAAGCTAGAC AGACAAGTTT ACAGCAGCAG CTTATCCGTG TGATGGACCG AATCTGTAAA
    TTAGTTGATG CTGTTCCTGA TGATGAGCTG AAAGCTTTGG ACTGTGGGAA CGAGCTGCTC
    CAGCAGCGGG ACATAAGAAG GAAACTCCTA GCTGAAGCGG ATTTTGATCC CAGTGATGCC
    AGTGTTCTTA GCGCCATGTG GGGGTGTAGG CCTGATCCCC TGGAGAGCCC TGTGAAGGAC
    TTGGCCTCTG TTTCCTTACC TTACGCGTTT CGGAAGGGTG ACTCCTGTCT TGCAGGGAAT
    GCTGTGAAGG AGTTTACTCT TCCACACCTC CCCTCAAATT CCATGTCCAC CCGGGAACGT
    TTACTGACTC CTCCCCTAGG AAAGACAGGA GTCTTAGCCA CCACAAAGAG TCCCTTTGAA
    AGACCGCTAT TCAGTTCCCA TTTACAGAAG TCCTTTGTAA GTAGCAACTG GGCTGAAACA
    CCGAGGGCAG AGAAAAGAAA TGAAAGCCCT GATTTCCCAG GAAATGTTCT CACAAGCACG
    GCTGTGAAAG ATCAGAATAA ATATACCACC TCCATAAATG ACTTGGAAAG AGAGCTCCAG
    GCTGCTTGCG AGATTGACAA TTTTGACATA GATGACTTTG ACGATGATGA TGATGACTGG
    GAAAATCTGA TGCAAAATTT AGCAGCCAGC AAATCTTCCA CAGCTGCCTA CCCACCCATT
    AAGGAAGGTC GACCAGTTAA ATCTGTGTCA GAAAGACCCT CTTCCGCCAA GACAGACTGC
    CTTCCAGGGG CATCTACCAC TCAAAATAAA AACTTCTCAG AGTCAATTCC GAATTACCCT
    GACAAGATGG AGCAGAATTT AGCATCCAGA AATCCGAAAC ATGAGCGTTT CCAGAGTCTT
    AATTTTCCTC ATACAAAAGA AATGATGAAG ATTTTTCATA AGAAATTTGG CCTGCATAAT
    TTTAGAACTA ATCAGCTAGA GGCGATCAAT GCTGCACTGC TTGGTGAAGA CTGTTTCATT
    TTAATGCCCA CTGGAGGTGG TAAAAGCCTG TGTTACCAGC TTCCTGCCTG TGTTTCTCCT
    GGGGTCACTA TTGTCATTTC CCCTTTGAGA TCACTTATAG TAGATCAAGT CCAAAAGCTG
    ACTTCCTTGG ATATCCCAGC TACATATCTG ACAGGTGATA AGACGGACTC AGAAGCTACA
    AGTATTTACC TCCAGTTATC AAAAAAAGAC CCAATTATAA AACTTCTGTA TGTTACGCCA
    GAGAAGGTCT GTGCGAGTAA CAGGCTGATC TCTACTCTGG AGAATCTGTA TGAGAGGAAG
    CTCTTGGCAC GTTTTGTCAT TGATGAAGCG CACTGTGTGA GTCAGTGGGG ACATGATTTT
    CGTCCAGATT ACAAAAGAAT GAATATTCTT CGCCAGAAGT TCCCTTCTGT TCCAGTGATG
    GCTCTCACTG CCACGGCCAA CCCCCGGGTA CAGAAGGACA TACTCACTCA GCTGAAGATT
    CTCAGGCCTC AGGTGTTTAG CATGAGCTTC AACAGACACA ATCTAAAATA CTACGTGTTA
    CCCAAAAAAC CTAAAAAGGT GGCATTTGAT TGCTTAGAAT GGATCAGAAA GCATCACCCA
    CATGACTCAG GGATAATTTA CTGCCTCTCC AGGCGAGAAT GTGACACAAT GGCTGAAACG
    TTACAGAACG ATGGCCTTGC TGCCCTGGCT TATCACGCCG GCCTCAGTGA CTCCGCCAGA
    GACGAAGTGC AGCACAAGTG GATTAATCAG GATGGCTGCC AGGTCATCTG CGCGACAATC
    GCCTTTGGAA TGGGCATCGA CAAACCGGAC GTGCGCTTTG TGATCCACGC CTCCCTCCCG
    AAGTCCGTGG AGGGTTACTA CCAGGAGTCT GGCCGAGCCG GAAGGGACGG GGAGATCTCT
    CACTGCATAC TCTTCTACAC CTACCACGAC GTGACCCGGC TGAAGAGGCT TATCCTGAGG
    AGTAAGAGTG CAAAAATTCA GTCAGGAATA TTTGGAAAAG GATCTACTTA TTCAAGACAC
    AACGCCGAAA GACTTTTTAA AAAACTGATA CTTGACAGGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGATCAAGC AATTGCCTAT GTGATGCTCG GAAGTAAAGC ACAAACCGTA
    CTAAATGGGC ATTTAAAGGT AGACTTTATG GAAACGGAAA ATTCCAGCAG TGTGAAAAAA
    CAAAAAGCGT TAGTGGCAAA GGTGTCTCAG AGGGAAGAGG TGGTGAAGAA ATGTCTTGGA
    GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GCGTCCACTA CTTTAATATT
    TTTAATACCG TCACTCTCAA GAAGCTCGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
    CTTCAAATTG ATGGTGTTAC CGAAGACAAA CTGGAAAAAT ATGGTGCTGA AGTGATTCCA
    ATATTACAGA AATACTCTGA GTGGACATTG CCAGCTGAGG ACACCTCCCC ACGGAGAAGC
    CCCTCCAGCA GCAGAGGCAC CAGAAAAAAC ACTCCTGAGG AGTCGGATGA GGGAGCACCC
    ATATCTTCTC ACTACTTTGC AAATAAAACC AGAAACGAAA GAAAGAGGAA AAGGATGCCA
    GCCTCCCAAA AAGCCAAGAG GAGAAGAACT GGTTTCAGTG GTTCCAGGAC GAAAGGGGGG
    TCCAACACAT GCCGAAGGAT ACCTTCTAAA ACTAAACCCT CCAACATAAT CGGACCCAGT
    TCCACTTCTC ATAGTTCTCA AGCAGCATCA GGAGTCAGTA GGAAACTGGG GATTATGGCT
    CCACCAAAGC CTATAAATAG ACCATTTCTG AAGCCTTCAT ATGCATTTTC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30869
    Clone ID Related Accession (Same CDS sequence) XM_002696583.5 , XM_613809.7
    Accession Version XM_002696583.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4293bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product Bloom syndrome protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002696583.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAACAACTGG AACGTCATTC GGCAAGAAAG 
    CTTAACAATA AATTGGGTCT TTCAAAACCG AAATCTTCAG GTTTCACTTT TAAAAAGAAA
    GTGCCTTCAG CCAACAATGT CTCTGTAACT AGTGTGTCAG TGGCAAAAAC ACCTGTGTTG
    AGTGATAAAG ATGTTAATAT CACTGAGGCC TTTTCCTTCA GTGAACCTTT ACCCCACACC
    ACCAATCAGC AGACGGGGAT CAATGACTTT AAAAGTGCTC CATCCGGACC GCAAACTAAG
    AGAGTTGGTT CAAAACCATT ATTGCCAAGT TTGTCGCAGG TTCCCCAGGA AGTTTCATGT
    ACGGCCCAGA ACACACCGGT TATAAAGCAA TCCCTCAATG CTACTTTCAA GAAATTAGAA
    TTTAGTTCCT CATCAGATTC TCTTATTGCC TTCAATGATT GGGATGATAT GGATGACTTC
    GATACTTCTG GAAATTCTAA AGCATTTGTG ACACCATGTA AAAACCACTT CGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGTCTAAG AGGAACTTAG TAAAAGCGCA GCTTAATAAA
    ACACATACAG GGGAGACTGA TTTGGCTCCC TCCTCCTCTG AGAGCAAGCA GGAACATTTG
    ACTGAGAAAC AGAAGGATGA CTCAGAATGG TTAAGTAGTG ATGTGATTTG CATTGATGAT
    GACCCCGATC CCGAAGAGCT GATAAATGAA GATACTCAGG AAAATCACTC GTTAAAAACT
    CATTTGGGAG CGGAAAGAGG TAATTGTGAA AAGAAGAAAA ACTTGGAAGA AACAGAATTA
    CGTTCAGTGG AGAAGTCTTC ATGTATTGAG CTTGACGAAG ATGATTATGA CATAGATTTT
    GTCCCACCTT CTCCAGAAGA AGGAATGACT TCTACCTCCT CATCCTCTTT AAAATGCTCT
    AGTATGTTGA AGGATCTTGA CACCTCTGAC AAAAAGCATA GTCTTAGCAC ATCAGAAGAC
    TTGTCAAAAC TCAAGGAAAT GGCCACACAA CAGCCTAATC AAGAAACCAG CACAAGCTGT
    GAAGCTAGAC AGACAAGTTT ACAGCAGCAG CTTATCCGTG TGATGGACCG AATCTGTAAA
    TTAGTTGATG CTGTTCCTGA TGATGAGCTG AAAGCTTTGG ACTGTGGGAA CGAGCTGCTC
    CAGCAGCGGG ACATAAGAAG GAAACTCCTA GCTGAAGCGG ATTTTGATCC CAGTGATGCC
    AGTGTTCTTA GCGCCATGTG GGGGTGTAGG CCTGATCCCC TGGAGAGCCC TGTGAAGGAC
    TTGGCCTCTG TTTCCTTACC TTACGCGTTT CGGAAGGGTG ACTCCTGTCT TGCAGGGAAT
    GCTGTGAAGG AGTTTACTCT TCCACACCTC CCCTCAAATT CCATGTCCAC CCGGGAACGT
    TTACTGACTC CTCCCCTAGG AAAGACAGGA GTCTTAGCCA CCACAAAGAG TCCCTTTGAA
    AGACCGCTAT TCAGTTCCCA TTTACAGAAG TCCTTTGTAA GTAGCAACTG GGCTGAAACA
    CCGAGGGCAG AGAAAAGAAA TGAAAGCCCT GATTTCCCAG GAAATGTTCT CACAAGCACG
    GCTGTGAAAG ATCAGAATAA ATATACCACC TCCATAAATG ACTTGGAAAG AGAGCTCCAG
    GCTGCTTGCG AGATTGACAA TTTTGACATA GATGACTTTG ACGATGATGA TGATGACTGG
    GAAAATCTGA TGCAAAATTT AGCAGCCAGC AAATCTTCCA CAGCTGCCTA CCCACCCATT
    AAGGAAGGTC GACCAGTTAA ATCTGTGTCA GAAAGACCCT CTTCCGCCAA GACAGACTGC
    CTTCCAGGGG CATCTACCAC TCAAAATAAA AACTTCTCAG AGTCAATTCC GAATTACCCT
    GACAAGATGG AGCAGAATTT AGCATCCAGA AATCCGAAAC ATGAGCGTTT CCAGAGTCTT
    AATTTTCCTC ATACAAAAGA AATGATGAAG ATTTTTCATA AGAAATTTGG CCTGCATAAT
    TTTAGAACTA ATCAGCTAGA GGCGATCAAT GCTGCACTGC TTGGTGAAGA CTGTTTCATT
    TTAATGCCCA CTGGAGGTGG TAAAAGCCTG TGTTACCAGC TTCCTGCCTG TGTTTCTCCT
    GGGGTCACTA TTGTCATTTC CCCTTTGAGA TCACTTATAG TAGATCAAGT CCAAAAGCTG
    ACTTCCTTGG ATATCCCAGC TACATATCTG ACAGGTGATA AGACGGACTC AGAAGCTACA
    AGTATTTACC TCCAGTTATC AAAAAAAGAC CCAATTATAA AACTTCTGTA TGTTACGCCA
    GAGAAGGTCT GTGCGAGTAA CAGGCTGATC TCTACTCTGG AGAATCTGTA TGAGAGGAAG
    CTCTTGGCAC GTTTTGTCAT TGATGAAGCG CACTGTGTGA GTCAGTGGGG ACATGATTTT
    CGTCCAGATT ACAAAAGAAT GAATATTCTT CGCCAGAAGT TCCCTTCTGT TCCAGTGATG
    GCTCTCACTG CCACGGCCAA CCCCCGGGTA CAGAAGGACA TACTCACTCA GCTGAAGATT
    CTCAGGCCTC AGGTGTTTAG CATGAGCTTC AACAGACACA ATCTAAAATA CTACGTGTTA
    CCCAAAAAAC CTAAAAAGGT GGCATTTGAT TGCTTAGAAT GGATCAGAAA GCATCACCCA
    CATGACTCAG GGATAATTTA CTGCCTCTCC AGGCGAGAAT GTGACACAAT GGCTGAAACG
    TTACAGAACG ATGGCCTTGC TGCCCTGGCT TATCACGCCG GCCTCAGTGA CTCCGCCAGA
    GACGAAGTGC AGCACAAGTG GATTAATCAG GATGGCTGCC AGGTCATCTG CGCGACAATC
    GCCTTTGGAA TGGGCATCGA CAAACCGGAC GTGCGCTTTG TGATCCACGC CTCCCTCCCG
    AAGTCCGTGG AGGGTTACTA CCAGGAGTCT GGCCGAGCCG GAAGGGACGG GGAGATCTCT
    CACTGCATAC TCTTCTACAC CTACCACGAC GTGACCCGGC TGAAGAGGCT TATCCTGATG
    GAAAAAGATG GAAACCGTCA TACACGAGAG ACCCACTTCA ATAATTTGTA TAGCATGGTA
    CATTACTGTG AAAATATAAC AGAATGTAGG AGAATACAGC TTTTGGCTTA CTTTGGCGAA
    AATGAATTTA ATCCTCATTT TTGTAAGAAA TACCCAGATG TTTCATGTGA TAACTGCTAT
    AAAACAAAGG ATTTTAAGAC AAAAGATGTG ACTGAAGATG TGAAAAATAT TGTAAGATTT
    GTTCAAGAAC ACAGTTCATC TCAGGGAACA AGAAATATAA AGCATATAGG TCCTTCTGGG
    AGGTTCACTA TGAACATGCT GGTTGACATT TTCTTGGGGA GTAAGAGTGC AAAAATTCAG
    TCAGGAATAT TTGGAAAAGG ATCTACTTAT TCAAGACACA ACGCCGAAAG ACTTTTTAAA
    AAACTGATAC TTGACAGGAT TTTGGATGAA GACTTATATA TCAATGCCAA TGATCAAGCA
    ATTGCCTATG TGATGCTCGG AAGTAAAGCA CAAACCGTAC TAAATGGGCA TTTAAAGGTA
    GACTTTATGG AAACGGAAAA TTCCAGCAGT GTGAAAAAAC AAAAAGCGTT AGTGGCAAAG
    GTGTCTCAGA GGGAAGAGGT GGTGAAGAAA TGTCTTGGAG AACTTACAGA AGTCTGCAAA
    TCTCTGGGGA AAGTTTTTGG CGTCCACTAC TTTAATATTT TTAATACCGT CACTCTCAAG
    AAGCTCGCAG AATCTTTATC TTCTGATCCT GAGGTTTTGC TTCAAATTGA TGGTGTTACC
    GAAGACAAAC TGGAAAAATA TGGTGCTGAA GTGATTCCAA TATTACAGAA ATACTCTGAG
    TGGACATTGC CAGCTGAGGA CACCTCCCCA CGGAGAAGCC CCTCCAGCAG CAGAGGCACC
    AGAAAAAACA CTCCTGAGGA GTCGGATGAG GGAGCACCCA TATCTTCTCA CTACTTTGCA
    AATAAAACCA GAAACGAAAG AAAGAGGAAA AGGATGCCAG CCTCCCAAAA AGCCAAGAGG
    AGAAGAACTG GTTTCAGTGG TTCCAGGACG AAAGGGGGGT CCAACACATG CCGAAGGATA
    CCTTCTAAAA CTAAACCCTC CAACATAATC GGACCCAGTT CCACTTCTCA TAGTTCTCAA
    GCAGCATCAG GAGTCAGTAG GAAACTGGGG ATTATGGCTC CACCAAAGCC TATAAATAGA
    CCATTTCTGA AGCCTTCATA TGCATTTTCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002696629.1
    CDS698..4990
    Translation

    Target ORF information:

    RefSeq Version XM_002696583.5
    Organism Bos taurus(cattle)
    Definition Bos taurus Bloom syndrome, RecQ helicase-like (BLM), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002696583.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAACAACTGG AACGTCATTC GGCAAGAAAG 
    CTTAACAATA AATTGGGTCT TTCAAAACCG AAATCTTCAG GTTTCACTTT TAAAAAGAAA
    GTGCCTTCAG CCAACAATGT CTCTGTAACT AGTGTGTCAG TGGCAAAAAC ACCTGTGTTG
    AGTGATAAAG ATGTTAATAT CACTGAGGCC TTTTCCTTCA GTGAACCTTT ACCCCACACC
    ACCAATCAGC AGACGGGGAT CAATGACTTT AAAAGTGCTC CATCCGGACC GCAAACTAAG
    AGAGTTGGTT CAAAACCATT ATTGCCAAGT TTGTCGCAGG TTCCCCAGGA AGTTTCATGT
    ACGGCCCAGA ACACACCGGT TATAAAGCAA TCCCTCAATG CTACTTTCAA GAAATTAGAA
    TTTAGTTCCT CATCAGATTC TCTTATTGCC TTCAATGATT GGGATGATAT GGATGACTTC
    GATACTTCTG GAAATTCTAA AGCATTTGTG ACACCATGTA AAAACCACTT CGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGTCTAAG AGGAACTTAG TAAAAGCGCA GCTTAATAAA
    ACACATACAG GGGAGACTGA TTTGGCTCCC TCCTCCTCTG AGAGCAAGCA GGAACATTTG
    ACTGAGAAAC AGAAGGATGA CTCAGAATGG TTAAGTAGTG ATGTGATTTG CATTGATGAT
    GACCCCGATC CCGAAGAGCT GATAAATGAA GATACTCAGG AAAATCACTC GTTAAAAACT
    CATTTGGGAG CGGAAAGAGG TAATTGTGAA AAGAAGAAAA ACTTGGAAGA AACAGAATTA
    CGTTCAGTGG AGAAGTCTTC ATGTATTGAG CTTGACGAAG ATGATTATGA CATAGATTTT
    GTCCCACCTT CTCCAGAAGA AGGAATGACT TCTACCTCCT CATCCTCTTT AAAATGCTCT
    AGTATGTTGA AGGATCTTGA CACCTCTGAC AAAAAGCATA GTCTTAGCAC ATCAGAAGAC
    TTGTCAAAAC TCAAGGAAAT GGCCACACAA CAGCCTAATC AAGAAACCAG CACAAGCTGT
    GAAGCTAGAC AGACAAGTTT ACAGCAGCAG CTTATCCGTG TGATGGACCG AATCTGTAAA
    TTAGTTGATG CTGTTCCTGA TGATGAGCTG AAAGCTTTGG ACTGTGGGAA CGAGCTGCTC
    CAGCAGCGGG ACATAAGAAG GAAACTCCTA GCTGAAGCGG ATTTTGATCC CAGTGATGCC
    AGTGTTCTTA GCGCCATGTG GGGGTGTAGG CCTGATCCCC TGGAGAGCCC TGTGAAGGAC
    TTGGCCTCTG TTTCCTTACC TTACGCGTTT CGGAAGGGTG ACTCCTGTCT TGCAGGGAAT
    GCTGTGAAGG AGTTTACTCT TCCACACCTC CCCTCAAATT CCATGTCCAC CCGGGAACGT
    TTACTGACTC CTCCCCTAGG AAAGACAGGA GTCTTAGCCA CCACAAAGAG TCCCTTTGAA
    AGACCGCTAT TCAGTTCCCA TTTACAGAAG TCCTTTGTAA GTAGCAACTG GGCTGAAACA
    CCGAGGGCAG AGAAAAGAAA TGAAAGCCCT GATTTCCCAG GAAATGTTCT CACAAGCACG
    GCTGTGAAAG ATCAGAATAA ATATACCACC TCCATAAATG ACTTGGAAAG AGAGCTCCAG
    GCTGCTTGCG AGATTGACAA TTTTGACATA GATGACTTTG ACGATGATGA TGATGACTGG
    GAAAATCTGA TGCAAAATTT AGCAGCCAGC AAATCTTCCA CAGCTGCCTA CCCACCCATT
    AAGGAAGGTC GACCAGTTAA ATCTGTGTCA GAAAGACCCT CTTCCGCCAA GACAGACTGC
    CTTCCAGGGG CATCTACCAC TCAAAATAAA AACTTCTCAG AGTCAATTCC GAATTACCCT
    GACAAGATGG AGCAGAATTT AGCATCCAGA AATCCGAAAC ATGAGCGTTT CCAGAGTCTT
    AATTTTCCTC ATACAAAAGA AATGATGAAG ATTTTTCATA AGAAATTTGG CCTGCATAAT
    TTTAGAACTA ATCAGCTAGA GGCGATCAAT GCTGCACTGC TTGGTGAAGA CTGTTTCATT
    TTAATGCCCA CTGGAGGTGG TAAAAGCCTG TGTTACCAGC TTCCTGCCTG TGTTTCTCCT
    GGGGTCACTA TTGTCATTTC CCCTTTGAGA TCACTTATAG TAGATCAAGT CCAAAAGCTG
    ACTTCCTTGG ATATCCCAGC TACATATCTG ACAGGTGATA AGACGGACTC AGAAGCTACA
    AGTATTTACC TCCAGTTATC AAAAAAAGAC CCAATTATAA AACTTCTGTA TGTTACGCCA
    GAGAAGGTCT GTGCGAGTAA CAGGCTGATC TCTACTCTGG AGAATCTGTA TGAGAGGAAG
    CTCTTGGCAC GTTTTGTCAT TGATGAAGCG CACTGTGTGA GTCAGTGGGG ACATGATTTT
    CGTCCAGATT ACAAAAGAAT GAATATTCTT CGCCAGAAGT TCCCTTCTGT TCCAGTGATG
    GCTCTCACTG CCACGGCCAA CCCCCGGGTA CAGAAGGACA TACTCACTCA GCTGAAGATT
    CTCAGGCCTC AGGTGTTTAG CATGAGCTTC AACAGACACA ATCTAAAATA CTACGTGTTA
    CCCAAAAAAC CTAAAAAGGT GGCATTTGAT TGCTTAGAAT GGATCAGAAA GCATCACCCA
    CATGACTCAG GGATAATTTA CTGCCTCTCC AGGCGAGAAT GTGACACAAT GGCTGAAACG
    TTACAGAACG ATGGCCTTGC TGCCCTGGCT TATCACGCCG GCCTCAGTGA CTCCGCCAGA
    GACGAAGTGC AGCACAAGTG GATTAATCAG GATGGCTGCC AGGTCATCTG CGCGACAATC
    GCCTTTGGAA TGGGCATCGA CAAACCGGAC GTGCGCTTTG TGATCCACGC CTCCCTCCCG
    AAGTCCGTGG AGGGTTACTA CCAGGAGTCT GGCCGAGCCG GAAGGGACGG GGAGATCTCT
    CACTGCATAC TCTTCTACAC CTACCACGAC GTGACCCGGC TGAAGAGGCT TATCCTGATG
    GAAAAAGATG GAAACCGTCA TACACGAGAG ACCCACTTCA ATAATTTGTA TAGCATGGTA
    CATTACTGTG AAAATATAAC AGAATGTAGG AGAATACAGC TTTTGGCTTA CTTTGGCGAA
    AATGAATTTA ATCCTCATTT TTGTAAGAAA TACCCAGATG TTTCATGTGA TAACTGCTAT
    AAAACAAAGG ATTTTAAGAC AAAAGATGTG ACTGAAGATG TGAAAAATAT TGTAAGATTT
    GTTCAAGAAC ACAGTTCATC TCAGGGAACA AGAAATATAA AGCATATAGG TCCTTCTGGG
    AGGTTCACTA TGAACATGCT GGTTGACATT TTCTTGGGGA GTAAGAGTGC AAAAATTCAG
    TCAGGAATAT TTGGAAAAGG ATCTACTTAT TCAAGACACA ACGCCGAAAG ACTTTTTAAA
    AAACTGATAC TTGACAGGAT TTTGGATGAA GACTTATATA TCAATGCCAA TGATCAAGCA
    ATTGCCTATG TGATGCTCGG AAGTAAAGCA CAAACCGTAC TAAATGGGCA TTTAAAGGTA
    GACTTTATGG AAACGGAAAA TTCCAGCAGT GTGAAAAAAC AAAAAGCGTT AGTGGCAAAG
    GTGTCTCAGA GGGAAGAGGT GGTGAAGAAA TGTCTTGGAG AACTTACAGA AGTCTGCAAA
    TCTCTGGGGA AAGTTTTTGG CGTCCACTAC TTTAATATTT TTAATACCGT CACTCTCAAG
    AAGCTCGCAG AATCTTTATC TTCTGATCCT GAGGTTTTGC TTCAAATTGA TGGTGTTACC
    GAAGACAAAC TGGAAAAATA TGGTGCTGAA GTGATTCCAA TATTACAGAA ATACTCTGAG
    TGGACATTGC CAGCTGAGGA CACCTCCCCA CGGAGAAGCC CCTCCAGCAG CAGAGGCACC
    AGAAAAAACA CTCCTGAGGA GTCGGATGAG GGAGCACCCA TATCTTCTCA CTACTTTGCA
    AATAAAACCA GAAACGAAAG AAAGAGGAAA AGGATGCCAG CCTCCCAAAA AGCCAAGAGG
    AGAAGAACTG GTTTCAGTGG TTCCAGGACG AAAGGGGGGT CCAACACATG CCGAAGGATA
    CCTTCTAAAA CTAAACCCTC CAACATAATC GGACCCAGTT CCACTTCTCA TAGTTCTCAA
    GCAGCATCAG GAGTCAGTAG GAAACTGGGG ATTATGGCTC CACCAAAGCC TATAAATAGA
    CCATTTCTGA AGCCTTCATA TGCATTTTCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30869
    Clone ID Related Accession (Same CDS sequence) XM_002696583.5 , XM_613809.7
    Accession Version XM_613809.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4293bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product Bloom syndrome protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646530.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_613809.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAACAACTGG AACGTCATTC GGCAAGAAAG 
    CTTAACAATA AATTGGGTCT TTCAAAACCG AAATCTTCAG GTTTCACTTT TAAAAAGAAA
    GTGCCTTCAG CCAACAATGT CTCTGTAACT AGTGTGTCAG TGGCAAAAAC ACCTGTGTTG
    AGTGATAAAG ATGTTAATAT CACTGAGGCC TTTTCCTTCA GTGAACCTTT ACCCCACACC
    ACCAATCAGC AGACGGGGAT CAATGACTTT AAAAGTGCTC CATCCGGACC GCAAACTAAG
    AGAGTTGGTT CAAAACCATT ATTGCCAAGT TTGTCGCAGG TTCCCCAGGA AGTTTCATGT
    ACGGCCCAGA ACACACCGGT TATAAAGCAA TCCCTCAATG CTACTTTCAA GAAATTAGAA
    TTTAGTTCCT CATCAGATTC TCTTATTGCC TTCAATGATT GGGATGATAT GGATGACTTC
    GATACTTCTG GAAATTCTAA AGCATTTGTG ACACCATGTA AAAACCACTT CGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGTCTAAG AGGAACTTAG TAAAAGCGCA GCTTAATAAA
    ACACATACAG GGGAGACTGA TTTGGCTCCC TCCTCCTCTG AGAGCAAGCA GGAACATTTG
    ACTGAGAAAC AGAAGGATGA CTCAGAATGG TTAAGTAGTG ATGTGATTTG CATTGATGAT
    GACCCCGATC CCGAAGAGCT GATAAATGAA GATACTCAGG AAAATCACTC GTTAAAAACT
    CATTTGGGAG CGGAAAGAGG TAATTGTGAA AAGAAGAAAA ACTTGGAAGA AACAGAATTA
    CGTTCAGTGG AGAAGTCTTC ATGTATTGAG CTTGACGAAG ATGATTATGA CATAGATTTT
    GTCCCACCTT CTCCAGAAGA AGGAATGACT TCTACCTCCT CATCCTCTTT AAAATGCTCT
    AGTATGTTGA AGGATCTTGA CACCTCTGAC AAAAAGCATA GTCTTAGCAC ATCAGAAGAC
    TTGTCAAAAC TCAAGGAAAT GGCCACACAA CAGCCTAATC AAGAAACCAG CACAAGCTGT
    GAAGCTAGAC AGACAAGTTT ACAGCAGCAG CTTATCCGTG TGATGGACCG AATCTGTAAA
    TTAGTTGATG CTGTTCCTGA TGATGAGCTG AAAGCTTTGG ACTGTGGGAA CGAGCTGCTC
    CAGCAGCGGG ACATAAGAAG GAAACTCCTA GCTGAAGCGG ATTTTGATCC CAGTGATGCC
    AGTGTTCTTA GCGCCATGTG GGGGTGTAGG CCTGATCCCC TGGAGAGCCC TGTGAAGGAC
    TTGGCCTCTG TTTCCTTACC TTACGCGTTT CGGAAGGGTG ACTCCTGTCT TGCAGGGAAT
    GCTGTGAAGG AGTTTACTCT TCCACACCTC CCCTCAAATT CCATGTCCAC CCGGGAACGT
    TTACTGACTC CTCCCCTAGG AAAGACAGGA GTCTTAGCCA CCACAAAGAG TCCCTTTGAA
    AGACCGCTAT TCAGTTCCCA TTTACAGAAG TCCTTTGTAA GTAGCAACTG GGCTGAAACA
    CCGAGGGCAG AGAAAAGAAA TGAAAGCCCT GATTTCCCAG GAAATGTTCT CACAAGCACG
    GCTGTGAAAG ATCAGAATAA ATATACCACC TCCATAAATG ACTTGGAAAG AGAGCTCCAG
    GCTGCTTGCG AGATTGACAA TTTTGACATA GATGACTTTG ACGATGATGA TGATGACTGG
    GAAAATCTGA TGCAAAATTT AGCAGCCAGC AAATCTTCCA CAGCTGCCTA CCCACCCATT
    AAGGAAGGTC GACCAGTTAA ATCTGTGTCA GAAAGACCCT CTTCCGCCAA GACAGACTGC
    CTTCCAGGGG CATCTACCAC TCAAAATAAA AACTTCTCAG AGTCAATTCC GAATTACCCT
    GACAAGATGG AGCAGAATTT AGCATCCAGA AATCCGAAAC ATGAGCGTTT CCAGAGTCTT
    AATTTTCCTC ATACAAAAGA AATGATGAAG ATTTTTCATA AGAAATTTGG CCTGCATAAT
    TTTAGAACTA ATCAGCTAGA GGCGATCAAT GCTGCACTGC TTGGTGAAGA CTGTTTCATT
    TTAATGCCCA CTGGAGGTGG TAAAAGCCTG TGTTACCAGC TTCCTGCCTG TGTTTCTCCT
    GGGGTCACTA TTGTCATTTC CCCTTTGAGA TCACTTATAG TAGATCAAGT CCAAAAGCTG
    ACTTCCTTGG ATATCCCAGC TACATATCTG ACAGGTGATA AGACGGACTC AGAAGCTACA
    AGTATTTACC TCCAGTTATC AAAAAAAGAC CCAATTATAA AACTTCTGTA TGTTACGCCA
    GAGAAGGTCT GTGCGAGTAA CAGGCTGATC TCTACTCTGG AGAATCTGTA TGAGAGGAAG
    CTCTTGGCAC GTTTTGTCAT TGATGAAGCG CACTGTGTGA GTCAGTGGGG ACATGATTTT
    CGTCCAGATT ACAAAAGAAT GAATATTCTT CGCCAGAAGT TCCCTTCTGT TCCAGTGATG
    GCTCTCACTG CCACGGCCAA CCCCCGGGTA CAGAAGGACA TACTCACTCA GCTGAAGATT
    CTCAGGCCTC AGGTGTTTAG CATGAGCTTC AACAGACACA ATCTAAAATA CTACGTGTTA
    CCCAAAAAAC CTAAAAAGGT GGCATTTGAT TGCTTAGAAT GGATCAGAAA GCATCACCCA
    CATGACTCAG GGATAATTTA CTGCCTCTCC AGGCGAGAAT GTGACACAAT GGCTGAAACG
    TTACAGAACG ATGGCCTTGC TGCCCTGGCT TATCACGCCG GCCTCAGTGA CTCCGCCAGA
    GACGAAGTGC AGCACAAGTG GATTAATCAG GATGGCTGCC AGGTCATCTG CGCGACAATC
    GCCTTTGGAA TGGGCATCGA CAAACCGGAC GTGCGCTTTG TGATCCACGC CTCCCTCCCG
    AAGTCCGTGG AGGGTTACTA CCAGGAGTCT GGCCGAGCCG GAAGGGACGG GGAGATCTCT
    CACTGCATAC TCTTCTACAC CTACCACGAC GTGACCCGGC TGAAGAGGCT TATCCTGATG
    GAAAAAGATG GAAACCGTCA TACACGAGAG ACCCACTTCA ATAATTTGTA TAGCATGGTA
    CATTACTGTG AAAATATAAC AGAATGTAGG AGAATACAGC TTTTGGCTTA CTTTGGCGAA
    AATGAATTTA ATCCTCATTT TTGTAAGAAA TACCCAGATG TTTCATGTGA TAACTGCTAT
    AAAACAAAGG ATTTTAAGAC AAAAGATGTG ACTGAAGATG TGAAAAATAT TGTAAGATTT
    GTTCAAGAAC ACAGTTCATC TCAGGGAACA AGAAATATAA AGCATATAGG TCCTTCTGGG
    AGGTTCACTA TGAACATGCT GGTTGACATT TTCTTGGGGA GTAAGAGTGC AAAAATTCAG
    TCAGGAATAT TTGGAAAAGG ATCTACTTAT TCAAGACACA ACGCCGAAAG ACTTTTTAAA
    AAACTGATAC TTGACAGGAT TTTGGATGAA GACTTATATA TCAATGCCAA TGATCAAGCA
    ATTGCCTATG TGATGCTCGG AAGTAAAGCA CAAACCGTAC TAAATGGGCA TTTAAAGGTA
    GACTTTATGG AAACGGAAAA TTCCAGCAGT GTGAAAAAAC AAAAAGCGTT AGTGGCAAAG
    GTGTCTCAGA GGGAAGAGGT GGTGAAGAAA TGTCTTGGAG AACTTACAGA AGTCTGCAAA
    TCTCTGGGGA AAGTTTTTGG CGTCCACTAC TTTAATATTT TTAATACCGT CACTCTCAAG
    AAGCTCGCAG AATCTTTATC TTCTGATCCT GAGGTTTTGC TTCAAATTGA TGGTGTTACC
    GAAGACAAAC TGGAAAAATA TGGTGCTGAA GTGATTCCAA TATTACAGAA ATACTCTGAG
    TGGACATTGC CAGCTGAGGA CACCTCCCCA CGGAGAAGCC CCTCCAGCAG CAGAGGCACC
    AGAAAAAACA CTCCTGAGGA GTCGGATGAG GGAGCACCCA TATCTTCTCA CTACTTTGCA
    AATAAAACCA GAAACGAAAG AAAGAGGAAA AGGATGCCAG CCTCCCAAAA AGCCAAGAGG
    AGAAGAACTG GTTTCAGTGG TTCCAGGACG AAAGGGGGGT CCAACACATG CCGAAGGATA
    CCTTCTAAAA CTAAACCCTC CAACATAATC GGACCCAGTT CCACTTCTCA TAGTTCTCAA
    GCAGCATCAG GAGTCAGTAG GAAACTGGGG ATTATGGCTC CACCAAAGCC TATAAATAGA
    CCATTTCTGA AGCCTTCATA TGCATTTTCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_613809.4
    CDS698..4990
    Translation

    Target ORF information:

    RefSeq Version XM_613809.7
    Organism Bos taurus(cattle)
    Definition Bos taurus Bloom syndrome, RecQ helicase-like (BLM), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_613809.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAACAACTGG AACGTCATTC GGCAAGAAAG 
    CTTAACAATA AATTGGGTCT TTCAAAACCG AAATCTTCAG GTTTCACTTT TAAAAAGAAA
    GTGCCTTCAG CCAACAATGT CTCTGTAACT AGTGTGTCAG TGGCAAAAAC ACCTGTGTTG
    AGTGATAAAG ATGTTAATAT CACTGAGGCC TTTTCCTTCA GTGAACCTTT ACCCCACACC
    ACCAATCAGC AGACGGGGAT CAATGACTTT AAAAGTGCTC CATCCGGACC GCAAACTAAG
    AGAGTTGGTT CAAAACCATT ATTGCCAAGT TTGTCGCAGG TTCCCCAGGA AGTTTCATGT
    ACGGCCCAGA ACACACCGGT TATAAAGCAA TCCCTCAATG CTACTTTCAA GAAATTAGAA
    TTTAGTTCCT CATCAGATTC TCTTATTGCC TTCAATGATT GGGATGATAT GGATGACTTC
    GATACTTCTG GAAATTCTAA AGCATTTGTG ACACCATGTA AAAACCACTT CGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGTCTAAG AGGAACTTAG TAAAAGCGCA GCTTAATAAA
    ACACATACAG GGGAGACTGA TTTGGCTCCC TCCTCCTCTG AGAGCAAGCA GGAACATTTG
    ACTGAGAAAC AGAAGGATGA CTCAGAATGG TTAAGTAGTG ATGTGATTTG CATTGATGAT
    GACCCCGATC CCGAAGAGCT GATAAATGAA GATACTCAGG AAAATCACTC GTTAAAAACT
    CATTTGGGAG CGGAAAGAGG TAATTGTGAA AAGAAGAAAA ACTTGGAAGA AACAGAATTA
    CGTTCAGTGG AGAAGTCTTC ATGTATTGAG CTTGACGAAG ATGATTATGA CATAGATTTT
    GTCCCACCTT CTCCAGAAGA AGGAATGACT TCTACCTCCT CATCCTCTTT AAAATGCTCT
    AGTATGTTGA AGGATCTTGA CACCTCTGAC AAAAAGCATA GTCTTAGCAC ATCAGAAGAC
    TTGTCAAAAC TCAAGGAAAT GGCCACACAA CAGCCTAATC AAGAAACCAG CACAAGCTGT
    GAAGCTAGAC AGACAAGTTT ACAGCAGCAG CTTATCCGTG TGATGGACCG AATCTGTAAA
    TTAGTTGATG CTGTTCCTGA TGATGAGCTG AAAGCTTTGG ACTGTGGGAA CGAGCTGCTC
    CAGCAGCGGG ACATAAGAAG GAAACTCCTA GCTGAAGCGG ATTTTGATCC CAGTGATGCC
    AGTGTTCTTA GCGCCATGTG GGGGTGTAGG CCTGATCCCC TGGAGAGCCC TGTGAAGGAC
    TTGGCCTCTG TTTCCTTACC TTACGCGTTT CGGAAGGGTG ACTCCTGTCT TGCAGGGAAT
    GCTGTGAAGG AGTTTACTCT TCCACACCTC CCCTCAAATT CCATGTCCAC CCGGGAACGT
    TTACTGACTC CTCCCCTAGG AAAGACAGGA GTCTTAGCCA CCACAAAGAG TCCCTTTGAA
    AGACCGCTAT TCAGTTCCCA TTTACAGAAG TCCTTTGTAA GTAGCAACTG GGCTGAAACA
    CCGAGGGCAG AGAAAAGAAA TGAAAGCCCT GATTTCCCAG GAAATGTTCT CACAAGCACG
    GCTGTGAAAG ATCAGAATAA ATATACCACC TCCATAAATG ACTTGGAAAG AGAGCTCCAG
    GCTGCTTGCG AGATTGACAA TTTTGACATA GATGACTTTG ACGATGATGA TGATGACTGG
    GAAAATCTGA TGCAAAATTT AGCAGCCAGC AAATCTTCCA CAGCTGCCTA CCCACCCATT
    AAGGAAGGTC GACCAGTTAA ATCTGTGTCA GAAAGACCCT CTTCCGCCAA GACAGACTGC
    CTTCCAGGGG CATCTACCAC TCAAAATAAA AACTTCTCAG AGTCAATTCC GAATTACCCT
    GACAAGATGG AGCAGAATTT AGCATCCAGA AATCCGAAAC ATGAGCGTTT CCAGAGTCTT
    AATTTTCCTC ATACAAAAGA AATGATGAAG ATTTTTCATA AGAAATTTGG CCTGCATAAT
    TTTAGAACTA ATCAGCTAGA GGCGATCAAT GCTGCACTGC TTGGTGAAGA CTGTTTCATT
    TTAATGCCCA CTGGAGGTGG TAAAAGCCTG TGTTACCAGC TTCCTGCCTG TGTTTCTCCT
    GGGGTCACTA TTGTCATTTC CCCTTTGAGA TCACTTATAG TAGATCAAGT CCAAAAGCTG
    ACTTCCTTGG ATATCCCAGC TACATATCTG ACAGGTGATA AGACGGACTC AGAAGCTACA
    AGTATTTACC TCCAGTTATC AAAAAAAGAC CCAATTATAA AACTTCTGTA TGTTACGCCA
    GAGAAGGTCT GTGCGAGTAA CAGGCTGATC TCTACTCTGG AGAATCTGTA TGAGAGGAAG
    CTCTTGGCAC GTTTTGTCAT TGATGAAGCG CACTGTGTGA GTCAGTGGGG ACATGATTTT
    CGTCCAGATT ACAAAAGAAT GAATATTCTT CGCCAGAAGT TCCCTTCTGT TCCAGTGATG
    GCTCTCACTG CCACGGCCAA CCCCCGGGTA CAGAAGGACA TACTCACTCA GCTGAAGATT
    CTCAGGCCTC AGGTGTTTAG CATGAGCTTC AACAGACACA ATCTAAAATA CTACGTGTTA
    CCCAAAAAAC CTAAAAAGGT GGCATTTGAT TGCTTAGAAT GGATCAGAAA GCATCACCCA
    CATGACTCAG GGATAATTTA CTGCCTCTCC AGGCGAGAAT GTGACACAAT GGCTGAAACG
    TTACAGAACG ATGGCCTTGC TGCCCTGGCT TATCACGCCG GCCTCAGTGA CTCCGCCAGA
    GACGAAGTGC AGCACAAGTG GATTAATCAG GATGGCTGCC AGGTCATCTG CGCGACAATC
    GCCTTTGGAA TGGGCATCGA CAAACCGGAC GTGCGCTTTG TGATCCACGC CTCCCTCCCG
    AAGTCCGTGG AGGGTTACTA CCAGGAGTCT GGCCGAGCCG GAAGGGACGG GGAGATCTCT
    CACTGCATAC TCTTCTACAC CTACCACGAC GTGACCCGGC TGAAGAGGCT TATCCTGATG
    GAAAAAGATG GAAACCGTCA TACACGAGAG ACCCACTTCA ATAATTTGTA TAGCATGGTA
    CATTACTGTG AAAATATAAC AGAATGTAGG AGAATACAGC TTTTGGCTTA CTTTGGCGAA
    AATGAATTTA ATCCTCATTT TTGTAAGAAA TACCCAGATG TTTCATGTGA TAACTGCTAT
    AAAACAAAGG ATTTTAAGAC AAAAGATGTG ACTGAAGATG TGAAAAATAT TGTAAGATTT
    GTTCAAGAAC ACAGTTCATC TCAGGGAACA AGAAATATAA AGCATATAGG TCCTTCTGGG
    AGGTTCACTA TGAACATGCT GGTTGACATT TTCTTGGGGA GTAAGAGTGC AAAAATTCAG
    TCAGGAATAT TTGGAAAAGG ATCTACTTAT TCAAGACACA ACGCCGAAAG ACTTTTTAAA
    AAACTGATAC TTGACAGGAT TTTGGATGAA GACTTATATA TCAATGCCAA TGATCAAGCA
    ATTGCCTATG TGATGCTCGG AAGTAAAGCA CAAACCGTAC TAAATGGGCA TTTAAAGGTA
    GACTTTATGG AAACGGAAAA TTCCAGCAGT GTGAAAAAAC AAAAAGCGTT AGTGGCAAAG
    GTGTCTCAGA GGGAAGAGGT GGTGAAGAAA TGTCTTGGAG AACTTACAGA AGTCTGCAAA
    TCTCTGGGGA AAGTTTTTGG CGTCCACTAC TTTAATATTT TTAATACCGT CACTCTCAAG
    AAGCTCGCAG AATCTTTATC TTCTGATCCT GAGGTTTTGC TTCAAATTGA TGGTGTTACC
    GAAGACAAAC TGGAAAAATA TGGTGCTGAA GTGATTCCAA TATTACAGAA ATACTCTGAG
    TGGACATTGC CAGCTGAGGA CACCTCCCCA CGGAGAAGCC CCTCCAGCAG CAGAGGCACC
    AGAAAAAACA CTCCTGAGGA GTCGGATGAG GGAGCACCCA TATCTTCTCA CTACTTTGCA
    AATAAAACCA GAAACGAAAG AAAGAGGAAA AGGATGCCAG CCTCCCAAAA AGCCAAGAGG
    AGAAGAACTG GTTTCAGTGG TTCCAGGACG AAAGGGGGGT CCAACACATG CCGAAGGATA
    CCTTCTAAAA CTAAACCCTC CAACATAATC GGACCCAGTT CCACTTCTCA TAGTTCTCAA
    GCAGCATCAG GAGTCAGTAG GAAACTGGGG ATTATGGCTC CACCAAAGCC TATAAATAGA
    CCATTTCTGA AGCCTTCATA TGCATTTTCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.