Blm cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Blm
Entrez Gene ID	12144
Full Name	Bloom syndrome, RecQ like helicase
General protein information	Preferred Names Bloom syndrome, RecQ like helicase Names Bloom syndrome protein homolog Bloom syndrome homolog Bloom syndrome, RecQ helicase-like mBLM recQ helicase homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 D2\|7 45.65 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017321956.1	XP_017177445.1	Bloom syndrome protein homolog isoform X1
XM_017321957.1	XP_017177446.1	Bloom syndrome protein homolog isoform X2
XM_017321957.2	XP_017177446.1	Bloom syndrome protein homolog isoform X2
NM_007550.4	NP_031576.4	Bloom syndrome protein homolog isoform 1
XM_017321956.2	XP_017177445.1	Bloom syndrome protein homolog isoform X1
NM_001042527.2	NP_001035992.1	Bloom syndrome protein homolog isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BLM	XP_510594.2
Canis lupus familiaris (dog)	BLM	XP_003434427.1
Mus musculus (house mouse)	Blm	NP_031576.4
	BLM	XP_001097543.1
Gallus gallus (chicken)	BLM	NP_001007088.2
Xenopus tropicalis (tropical clawed frog)	blm	NP_001243837.1
Homo sapiens (human)	BLM	NP_000048.1
Bos taurus (cattle)	BLM	XP_002696629.1
Rattus norvegicus (Norway rat)	Blm	XP_006229520.1
Danio rerio (zebrafish)	blm	XP_701357.4

Gene Ontology
Bibliography

Related articles in PubMed

BLM helicase suppresses recombination at G-quadruplex motifs in transcribed genes.
van Wietmarschen N, Merzouk S, Halsema N, Spierings DCJ, Guryev V, Lansdorp PM
Nature communications9(1)271(2018 Jan)

BLM helicase regulates DNA repair by counteracting RAD51 loading at DNA double-strand break sites.
Patel DS, Misenko SM, Her J, Bunting SF
The Journal of cell biology216(11)3521-3534(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The anti-recombinase activity of BLM is of general importance for normal retention of RAD51 at DNA double strand break sites and regulation of homologous recombination.
Title: BLM helicase regulates DNA repair by counteracting RAD51 loading at DNA double-strand break sites.

BLM helicase facilites telomere replication by resolving G4 structures formed during copying of the G-rich strand by leading strand synthesis.
Title: BLM helicase facilitates telomere replication during leading strand synthesis of telomeres.

Data suggest that BLM suppresses homologous recombination, while full-length BRCA1 promotes this process.
Title: A conditional mouse model for measuring the frequency of homologous recombination events in vivo in the absence of essential genes.

Although interhomolog recombination is slightly decreased in the absence of BLM, loss of heterozygosity is increased by fivefold or more, implying significantly increased interhomolog crossing over.
Title: Interhomolog recombination and loss of heterozygosity in wild-type and Bloom syndrome helicase (BLM)-deficient mammalian cells.

Blm has roles both upstream and downstream of the Rad54 protein, a core HR factor. Disruption of Rad54 in the Blm-mutant background reduced the elevated level of gene targeting and of sister chromatid exchanges.
Title: BLM has early and late functions in homologous recombination repair in mouse embryonic stem cells.

The following Blm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Blm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu81912	XM_017321956.2 Latest version!	Mus musculus Bloom syndrome, RecQ like helicase (Blm), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu81913	XM_017321957.2 Latest version!	Mus musculus Bloom syndrome, RecQ like helicase (Blm), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu15901	NM_001042527.2 Latest version!	Mus musculus Bloom syndrome, RecQ like helicase (Blm), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu16148	NM_007550.4 Latest version!	Mus musculus Bloom syndrome, RecQ like helicase (Blm), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$832.30 $1189.00
View Old Accession Versions >>
OMu81913	XM_017321957.1	Mus musculus Bloom syndrome, RecQ helicase-like (Blm), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu81912	XM_017321956.1	Mus musculus Bloom syndrome, RecQ helicase-like (Blm), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Blm ( XM_017321956.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177445.1 Mus musculus Bloom syndrome, RecQ helicase-like (Blm), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu81912
Clone ID Related Accession (Same CDS sequence)	XM_017321956.1 , XM_017321956.2
Accession Version	XM_017321956.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	Bloom syndrome protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG TTCCTCTGAA CAATCTACAA GAACAACTAC AGCGACACTC AGCCAGAAAA 
CTTAATAATC AACCCAGCCT TTCAAAACCA AAATCTTTAG GTTTTACTTT TAAAAAGAAA 
ACATCAGAGG GTGATGTGTC TGTCACTAGT GTGTCCGTAG TAAAAACACC TGCGTTAAGT 
GATAAAGATG TGAACGTGTC TGAGGCCTTT TCATTCACTG AGTCTCCACT CCACAAACCA 
AAGCAGCAGG CAAAGATTGA AGGCTTCTTT AAACATTTCC CTGGAAGGCA GCAAAGCAAG 
GGGACCTGCT CTGAGCCGTC ACTGCCGGCC ACGGTACAGA CTGCTCAGGA CACTTTGTGC 
ACTACCCCCA AAACCCCCAC TGCGAAGAAA CTGCCCGTGG CTGTTTTCAA GAAATTAGAA 
TTTAGTTCTT CTGCAGACTC CCTCAGTGAC TGGGCTGATA TGGATGACTT TGATATGTCA 
GCATCAGATG CGTTTGCTTC ACTGGCTAAA AATCCTGCCA CAAGAGTAAG CACCGCTCAG 
AAAATGAAAA AGACTAAGAG AAACTTCTTT AAACCACCAC CTCGTAAAGC CAATGCAGTA 
AAGACTGACT TGACTCCTCC CTCCCCCGAA TGCCTGCAAG TGGATTTAAC GAAGGAATCG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGCG GAGGGGGCGG ACTGCCTGAG CAGGGATGTG 
ATCTGCATTG ACAATGATTC TGCTTCTGAA GAGCTCACGG AGAAAGACAC GCAGGAAAGC 
CAGTCTTTGA AAGCTCACTT GGGAGCTGAA AGAGGTGACA GTGAAAAGAA GAGCCATGAA 
GACGAAGCTG TGTTCCATTC AGTTCAGAAC ACTGAATACT TTGAACACAA TGACAATGAT 
TATGATATAG ATTTTGTTCC ACCTTCTCCA GAAGAAATCA TCTCCACTGC TTCTTCCTCG 
TTGAAATGTT CCAGTATGTT AAAGGATCTT GATGACTCTG ACAAAGAAAA GGGCATTCTT 
AGCACCTCAG AAGAGCTTCT GTCAAAACCA GAGGAAATGA CCACACACAA GTCTGATGCA 
GGAACCAGTA AAGACTGTGA TGCCCAGCAG ATACGCATAC AGCAGCAGCT TATTCATGTG 
ATGGAGCACA TCTGTAAGTT AGTTGATACT GTTCCTACTG ATGAACTGGA AGCTTTGAAT 
TGTGGGACCG AATTGCTTCA ACAACGAAAC ATAAGGAGGA AGCTCCTAGC TGAAGCAGGT 
TTTAATGGAA ATGACGTCAG ACTTCTGGGT TCTCTGTGGA GGCACAGGCC TGATTCACTT 
GATAACACAG TGCAGGGCGA CTCCTGCCCT GTGGGGCATC CTAATAAAGA GTTAAATTCT 
CCATACCTTC TCTCACATTC CCCTTCCACT GAGGAATGTT TACCCACCAC CACTCCAGGA 
AAGACAGGAT TCTCAGCCAC CCCGAAGAAT CTCTTTGAAA GGCCGTTATT GAATTCCCAT 
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGGATGGA AAACAGGAAC 
GAAAGCACTG ACTTCCCAGG GAGTGTTCTC ACCAGCACCA CTGTGAAAGC TCAGAGTAAA 
CAAGCTGCTT CAGGATGGAA CGTAGAGAGA CACGGCCAGG CTTCCTATGA TATCGATAAC 
TTTAATATTG ATGACTTTGA TGATGATGAT GATGATGATG ACTGGGAAAA CATAATGCAC 
AATTTTCCAG CCAGCAAATC TTCCACAGCC ACCTACCCAC CCATCAAGGA AGGTGGGCCA 
GTTAAATCTC TCTCAGAAAG GATTTCTTCA GCCAAGGCAA AGTTTCTTCC AGTGGTATCA 
ACCGCTCAAA ATACAAACCT CTCAGAGTCA ATTCAGAATT GCTCTGATAA GCTGGCCCAA 
AATTTATCAT CAAAAAATCC AAAACATGAA CATTTTCAAA GTCTTAATTT TCCTCATACA 
AAAGAAATGA TGAAGATTTT CCATAAGAAA TTTGGCTTGC ATAATTTTAG AACTAATCAG 
CTAGAGGCGA TCAATGCTGC GCTGCTTGGT GAAGACTGCT TTATCCTAAT GCCCACTGGA 
GGAGGTAAAA GTTTGTGCTA CCAGCTCCCT GCCTGTGTTT CTCCTGGGGT CACAATTGTC 
ATTTCTCCCT TGAGATCACT AATAGTAGAT CAAGTCCAAA AGCTGACTTC CTTTGATATT 
CCAGCTACAT ATCTGACAGG GGATAAGACT GACTCAGAAG CTGCAAATAT TTACCTCCAA 
TTATCCAAAA AAGACCCAAT CATCAAGCTT TTGTATGTTA CTCCAGAGAA GGTCTGTGCA 
AGTAACAGGC TGATTTCTAC TCTGGAGAAT CTGTATGAGC GGAAGCTCTT GGCACGTTTT 
GTCATTGATG AAGCGCATTG TGTGAGTCAG TGGGGTCATG ATTTTCGTCA AGATTACAAA 
AGGATGAATA TGCTTCGCCA GAAGTTTCCT TCTGTTCCAG TGATGGCCCT CACGGCCACA 
GCGAACCCCA GGGTCCAGAA GGACATCCTC ACTCAGCTGA AGATCCTCAG ACCTCAGGTG 
TTTAGCATGA GCTTTAACAG ACACAATCTG AAGTACTATG TATTACCCAA GAAGCCCAAA 
AAAGTAGCAT TTGATTGCCT AGAGTGGATC AGAAAGCATC ACCCTTATGA CTCGGGGATA 
ATTTACTGCC TCTCCAGGAG GGAATGTGAC ACAATGGCTG ACACTTTACA GAGAGAAGGC 
CTGGCTGCCC TGGCTTACCA TGCGGGCCTC AGTGACTCTG CCAGAGATGA GGTGCAGCAC 
AAGTGGATCA ACCAGGACAA CTGCCAGTGG AAAAAGATGG AAACTATCAT ACGAAGGAAA 
CTCACGTCAA TAATCTATAT AGCATGGTAC ATTACTGTGA AAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177445.1
CDS	110..3037
Translation	MAAVPLNNLQEQLQRHSARKLNNQPSLSKPKSLGFTFKKKTSEGDVSVTSVSVVKTPALSDKDVNVSEAFSFTESPLHKPKQQAKIEGFFKHFPGRQQSKGTCSEPSLPATVQTAQDTLCTTPKTPTAKKLPVAVFKKLEFSSSADSLSDWADMDDFDMSASDAFASLAKNPATRVSTAQKMKKTKRNFFKPPPRKANAVKTDLTPPSPECLQVDLTKESEEEEEEEEEAEGADCLSRDVICIDNDSASEELTEKDTQESQSLKAHLGAERGDSEKKSHEDEAVFHSVQNTEYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKGILSTSEELLSKPEEMTTHKSDAGTSKDCDAQQIRIQQQLIHVMEHICKLVDTVPTDELEALNCGTELLQQRNIRRKLLAEAGFNGNDVRLLGSLWRHRPDSLDNTVQGDSCPVGHPNKELNSPYLLSHSPSTEECLPTTTPGKTGFSATPKNLFERPLLNSHLQKSFVSSNWAETPRMENRNESTDFPGSVLTSTTVKAQSKQAASGWNVERHGQASYDIDNFNIDDFDDDDDDDDWENIMHNFPASKSSTATYPPIKEGGPVKSLSERISSAKAKFLPVVSTAQNTNLSESIQNCSDKLAQNLSSKNPKHEHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQWKKMETIIRRKLTSIIYIAWYITVKT

Target ORF information:

RefSeq Version	XM_017321956.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bloom syndrome, RecQ helicase-like (Blm), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321956.1

ORF Insert Sequence:

ATGGCTGCTG TTCCTCTGAA CAATCTACAA GAACAACTAC AGCGACACTC AGCCAGAAAA 
CTTAATAATC AACCCAGCCT TTCAAAACCA AAATCTTTAG GTTTTACTTT TAAAAAGAAA 
ACATCAGAGG GTGATGTGTC TGTCACTAGT GTGTCCGTAG TAAAAACACC TGCGTTAAGT 
GATAAAGATG TGAACGTGTC TGAGGCCTTT TCATTCACTG AGTCTCCACT CCACAAACCA 
AAGCAGCAGG CAAAGATTGA AGGCTTCTTT AAACATTTCC CTGGAAGGCA GCAAAGCAAG 
GGGACCTGCT CTGAGCCGTC ACTGCCGGCC ACGGTACAGA CTGCTCAGGA CACTTTGTGC 
ACTACCCCCA AAACCCCCAC TGCGAAGAAA CTGCCCGTGG CTGTTTTCAA GAAATTAGAA 
TTTAGTTCTT CTGCAGACTC CCTCAGTGAC TGGGCTGATA TGGATGACTT TGATATGTCA 
GCATCAGATG CGTTTGCTTC ACTGGCTAAA AATCCTGCCA CAAGAGTAAG CACCGCTCAG 
AAAATGAAAA AGACTAAGAG AAACTTCTTT AAACCACCAC CTCGTAAAGC CAATGCAGTA 
AAGACTGACT TGACTCCTCC CTCCCCCGAA TGCCTGCAAG TGGATTTAAC GAAGGAATCG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGCG GAGGGGGCGG ACTGCCTGAG CAGGGATGTG 
ATCTGCATTG ACAATGATTC TGCTTCTGAA GAGCTCACGG AGAAAGACAC GCAGGAAAGC 
CAGTCTTTGA AAGCTCACTT GGGAGCTGAA AGAGGTGACA GTGAAAAGAA GAGCCATGAA 
GACGAAGCTG TGTTCCATTC AGTTCAGAAC ACTGAATACT TTGAACACAA TGACAATGAT 
TATGATATAG ATTTTGTTCC ACCTTCTCCA GAAGAAATCA TCTCCACTGC TTCTTCCTCG 
TTGAAATGTT CCAGTATGTT AAAGGATCTT GATGACTCTG ACAAAGAAAA GGGCATTCTT 
AGCACCTCAG AAGAGCTTCT GTCAAAACCA GAGGAAATGA CCACACACAA GTCTGATGCA 
GGAACCAGTA AAGACTGTGA TGCCCAGCAG ATACGCATAC AGCAGCAGCT TATTCATGTG 
ATGGAGCACA TCTGTAAGTT AGTTGATACT GTTCCTACTG ATGAACTGGA AGCTTTGAAT 
TGTGGGACCG AATTGCTTCA ACAACGAAAC ATAAGGAGGA AGCTCCTAGC TGAAGCAGGT 
TTTAATGGAA ATGACGTCAG ACTTCTGGGT TCTCTGTGGA GGCACAGGCC TGATTCACTT 
GATAACACAG TGCAGGGCGA CTCCTGCCCT GTGGGGCATC CTAATAAAGA GTTAAATTCT 
CCATACCTTC TCTCACATTC CCCTTCCACT GAGGAATGTT TACCCACCAC CACTCCAGGA 
AAGACAGGAT TCTCAGCCAC CCCGAAGAAT CTCTTTGAAA GGCCGTTATT GAATTCCCAT 
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGGATGGA AAACAGGAAC 
GAAAGCACTG ACTTCCCAGG GAGTGTTCTC ACCAGCACCA CTGTGAAAGC TCAGAGTAAA 
CAAGCTGCTT CAGGATGGAA CGTAGAGAGA CACGGCCAGG CTTCCTATGA TATCGATAAC 
TTTAATATTG ATGACTTTGA TGATGATGAT GATGATGATG ACTGGGAAAA CATAATGCAC 
AATTTTCCAG CCAGCAAATC TTCCACAGCC ACCTACCCAC CCATCAAGGA AGGTGGGCCA 
GTTAAATCTC TCTCAGAAAG GATTTCTTCA GCCAAGGCAA AGTTTCTTCC AGTGGTATCA 
ACCGCTCAAA ATACAAACCT CTCAGAGTCA ATTCAGAATT GCTCTGATAA GCTGGCCCAA 
AATTTATCAT CAAAAAATCC AAAACATGAA CATTTTCAAA GTCTTAATTT TCCTCATACA 
AAAGAAATGA TGAAGATTTT CCATAAGAAA TTTGGCTTGC ATAATTTTAG AACTAATCAG 
CTAGAGGCGA TCAATGCTGC GCTGCTTGGT GAAGACTGCT TTATCCTAAT GCCCACTGGA 
GGAGGTAAAA GTTTGTGCTA CCAGCTCCCT GCCTGTGTTT CTCCTGGGGT CACAATTGTC 
ATTTCTCCCT TGAGATCACT AATAGTAGAT CAAGTCCAAA AGCTGACTTC CTTTGATATT 
CCAGCTACAT ATCTGACAGG GGATAAGACT GACTCAGAAG CTGCAAATAT TTACCTCCAA 
TTATCCAAAA AAGACCCAAT CATCAAGCTT TTGTATGTTA CTCCAGAGAA GGTCTGTGCA 
AGTAACAGGC TGATTTCTAC TCTGGAGAAT CTGTATGAGC GGAAGCTCTT GGCACGTTTT 
GTCATTGATG AAGCGCATTG TGTGAGTCAG TGGGGTCATG ATTTTCGTCA AGATTACAAA 
AGGATGAATA TGCTTCGCCA GAAGTTTCCT TCTGTTCCAG TGATGGCCCT CACGGCCACA 
GCGAACCCCA GGGTCCAGAA GGACATCCTC ACTCAGCTGA AGATCCTCAG ACCTCAGGTG 
TTTAGCATGA GCTTTAACAG ACACAATCTG AAGTACTATG TATTACCCAA GAAGCCCAAA 
AAAGTAGCAT TTGATTGCCT AGAGTGGATC AGAAAGCATC ACCCTTATGA CTCGGGGATA 
ATTTACTGCC TCTCCAGGAG GGAATGTGAC ACAATGGCTG ACACTTTACA GAGAGAAGGC 
CTGGCTGCCC TGGCTTACCA TGCGGGCCTC AGTGACTCTG CCAGAGATGA GGTGCAGCAC 
AAGTGGATCA ACCAGGACAA CTGCCAGTGG AAAAAGATGG AAACTATCAT ACGAAGGAAA 
CTCACGTCAA TAATCTATAT AGCATGGTAC ATTACTGTGA AAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blm ( XM_017321956.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177445.1 Mus musculus Bloom syndrome, RecQ like helicase (Blm), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu81912
Clone ID Related Accession (Same CDS sequence)	XM_017321956.1 , XM_017321956.2
Accession Version	XM_017321956.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	Bloom syndrome protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG TTCCTCTGAA CAATCTACAA GAACAACTAC AGCGACACTC AGCCAGAAAA 
CTTAATAATC AACCCAGCCT TTCAAAACCA AAATCTTTAG GTTTTACTTT TAAAAAGAAA 
ACATCAGAGG GTGATGTGTC TGTCACTAGT GTGTCCGTAG TAAAAACACC TGCGTTAAGT 
GATAAAGATG TGAACGTGTC TGAGGCCTTT TCATTCACTG AGTCTCCACT CCACAAACCA 
AAGCAGCAGG CAAAGATTGA AGGCTTCTTT AAACATTTCC CTGGAAGGCA GCAAAGCAAG 
GGGACCTGCT CTGAGCCGTC ACTGCCGGCC ACGGTACAGA CTGCTCAGGA CACTTTGTGC 
ACTACCCCCA AAACCCCCAC TGCGAAGAAA CTGCCCGTGG CTGTTTTCAA GAAATTAGAA 
TTTAGTTCTT CTGCAGACTC CCTCAGTGAC TGGGCTGATA TGGATGACTT TGATATGTCA 
GCATCAGATG CGTTTGCTTC ACTGGCTAAA AATCCTGCCA CAAGAGTAAG CACCGCTCAG 
AAAATGAAAA AGACTAAGAG AAACTTCTTT AAACCACCAC CTCGTAAAGC CAATGCAGTA 
AAGACTGACT TGACTCCTCC CTCCCCCGAA TGCCTGCAAG TGGATTTAAC GAAGGAATCG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGCG GAGGGGGCGG ACTGCCTGAG CAGGGATGTG 
ATCTGCATTG ACAATGATTC TGCTTCTGAA GAGCTCACGG AGAAAGACAC GCAGGAAAGC 
CAGTCTTTGA AAGCTCACTT GGGAGCTGAA AGAGGTGACA GTGAAAAGAA GAGCCATGAA 
GACGAAGCTG TGTTCCATTC AGTTCAGAAC ACTGAATACT TTGAACACAA TGACAATGAT 
TATGATATAG ATTTTGTTCC ACCTTCTCCA GAAGAAATCA TCTCCACTGC TTCTTCCTCG 
TTGAAATGTT CCAGTATGTT AAAGGATCTT GATGACTCTG ACAAAGAAAA GGGCATTCTT 
AGCACCTCAG AAGAGCTTCT GTCAAAACCA GAGGAAATGA CCACACACAA GTCTGATGCA 
GGAACCAGTA AAGACTGTGA TGCCCAGCAG ATACGCATAC AGCAGCAGCT TATTCATGTG 
ATGGAGCACA TCTGTAAGTT AGTTGATACT GTTCCTACTG ATGAACTGGA AGCTTTGAAT 
TGTGGGACCG AATTGCTTCA ACAACGAAAC ATAAGGAGGA AGCTCCTAGC TGAAGCAGGT 
TTTAATGGAA ATGACGTCAG ACTTCTGGGT TCTCTGTGGA GGCACAGGCC TGATTCACTT 
GATAACACAG TGCAGGGCGA CTCCTGCCCT GTGGGGCATC CTAATAAAGA GTTAAATTCT 
CCATACCTTC TCTCACATTC CCCTTCCACT GAGGAATGTT TACCCACCAC CACTCCAGGA 
AAGACAGGAT TCTCAGCCAC CCCGAAGAAT CTCTTTGAAA GGCCGTTATT GAATTCCCAT 
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGGATGGA AAACAGGAAC 
GAAAGCACTG ACTTCCCAGG GAGTGTTCTC ACCAGCACCA CTGTGAAAGC TCAGAGTAAA 
CAAGCTGCTT CAGGATGGAA CGTAGAGAGA CACGGCCAGG CTTCCTATGA TATCGATAAC 
TTTAATATTG ATGACTTTGA TGATGATGAT GATGATGATG ACTGGGAAAA CATAATGCAC 
AATTTTCCAG CCAGCAAATC TTCCACAGCC ACCTACCCAC CCATCAAGGA AGGTGGGCCA 
GTTAAATCTC TCTCAGAAAG GATTTCTTCA GCCAAGGCAA AGTTTCTTCC AGTGGTATCA 
ACCGCTCAAA ATACAAACCT CTCAGAGTCA ATTCAGAATT GCTCTGATAA GCTGGCCCAA 
AATTTATCAT CAAAAAATCC AAAACATGAA CATTTTCAAA GTCTTAATTT TCCTCATACA 
AAAGAAATGA TGAAGATTTT CCATAAGAAA TTTGGCTTGC ATAATTTTAG AACTAATCAG 
CTAGAGGCGA TCAATGCTGC GCTGCTTGGT GAAGACTGCT TTATCCTAAT GCCCACTGGA 
GGAGGTAAAA GTTTGTGCTA CCAGCTCCCT GCCTGTGTTT CTCCTGGGGT CACAATTGTC 
ATTTCTCCCT TGAGATCACT AATAGTAGAT CAAGTCCAAA AGCTGACTTC CTTTGATATT 
CCAGCTACAT ATCTGACAGG GGATAAGACT GACTCAGAAG CTGCAAATAT TTACCTCCAA 
TTATCCAAAA AAGACCCAAT CATCAAGCTT TTGTATGTTA CTCCAGAGAA GGTCTGTGCA 
AGTAACAGGC TGATTTCTAC TCTGGAGAAT CTGTATGAGC GGAAGCTCTT GGCACGTTTT 
GTCATTGATG AAGCGCATTG TGTGAGTCAG TGGGGTCATG ATTTTCGTCA AGATTACAAA 
AGGATGAATA TGCTTCGCCA GAAGTTTCCT TCTGTTCCAG TGATGGCCCT CACGGCCACA 
GCGAACCCCA GGGTCCAGAA GGACATCCTC ACTCAGCTGA AGATCCTCAG ACCTCAGGTG 
TTTAGCATGA GCTTTAACAG ACACAATCTG AAGTACTATG TATTACCCAA GAAGCCCAAA 
AAAGTAGCAT TTGATTGCCT AGAGTGGATC AGAAAGCATC ACCCTTATGA CTCGGGGATA 
ATTTACTGCC TCTCCAGGAG GGAATGTGAC ACAATGGCTG ACACTTTACA GAGAGAAGGC 
CTGGCTGCCC TGGCTTACCA TGCGGGCCTC AGTGACTCTG CCAGAGATGA GGTGCAGCAC 
AAGTGGATCA ACCAGGACAA CTGCCAGTGG AAAAAGATGG AAACTATCAT ACGAAGGAAA 
CTCACGTCAA TAATCTATAT AGCATGGTAC ATTACTGTGA AAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177445.1
CDS	111..3038
Translation	MAAVPLNNLQEQLQRHSARKLNNQPSLSKPKSLGFTFKKKTSEGDVSVTSVSVVKTPALSDKDVNVSEAFSFTESPLHKPKQQAKIEGFFKHFPGRQQSKGTCSEPSLPATVQTAQDTLCTTPKTPTAKKLPVAVFKKLEFSSSADSLSDWADMDDFDMSASDAFASLAKNPATRVSTAQKMKKTKRNFFKPPPRKANAVKTDLTPPSPECLQVDLTKESEEEEEEEEEAEGADCLSRDVICIDNDSASEELTEKDTQESQSLKAHLGAERGDSEKKSHEDEAVFHSVQNTEYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKGILSTSEELLSKPEEMTTHKSDAGTSKDCDAQQIRIQQQLIHVMEHICKLVDTVPTDELEALNCGTELLQQRNIRRKLLAEAGFNGNDVRLLGSLWRHRPDSLDNTVQGDSCPVGHPNKELNSPYLLSHSPSTEECLPTTTPGKTGFSATPKNLFERPLLNSHLQKSFVSSNWAETPRMENRNESTDFPGSVLTSTTVKAQSKQAASGWNVERHGQASYDIDNFNIDDFDDDDDDDDWENIMHNFPASKSSTATYPPIKEGGPVKSLSERISSAKAKFLPVVSTAQNTNLSESIQNCSDKLAQNLSSKNPKHEHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQWKKMETIIRRKLTSIIYIAWYITVKT

Target ORF information:

RefSeq Version	XM_017321956.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bloom syndrome, RecQ like helicase (Blm), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321956.2

ORF Insert Sequence:

ATGGCTGCTG TTCCTCTGAA CAATCTACAA GAACAACTAC AGCGACACTC AGCCAGAAAA 
CTTAATAATC AACCCAGCCT TTCAAAACCA AAATCTTTAG GTTTTACTTT TAAAAAGAAA 
ACATCAGAGG GTGATGTGTC TGTCACTAGT GTGTCCGTAG TAAAAACACC TGCGTTAAGT 
GATAAAGATG TGAACGTGTC TGAGGCCTTT TCATTCACTG AGTCTCCACT CCACAAACCA 
AAGCAGCAGG CAAAGATTGA AGGCTTCTTT AAACATTTCC CTGGAAGGCA GCAAAGCAAG 
GGGACCTGCT CTGAGCCGTC ACTGCCGGCC ACGGTACAGA CTGCTCAGGA CACTTTGTGC 
ACTACCCCCA AAACCCCCAC TGCGAAGAAA CTGCCCGTGG CTGTTTTCAA GAAATTAGAA 
TTTAGTTCTT CTGCAGACTC CCTCAGTGAC TGGGCTGATA TGGATGACTT TGATATGTCA 
GCATCAGATG CGTTTGCTTC ACTGGCTAAA AATCCTGCCA CAAGAGTAAG CACCGCTCAG 
AAAATGAAAA AGACTAAGAG AAACTTCTTT AAACCACCAC CTCGTAAAGC CAATGCAGTA 
AAGACTGACT TGACTCCTCC CTCCCCCGAA TGCCTGCAAG TGGATTTAAC GAAGGAATCG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGCG GAGGGGGCGG ACTGCCTGAG CAGGGATGTG 
ATCTGCATTG ACAATGATTC TGCTTCTGAA GAGCTCACGG AGAAAGACAC GCAGGAAAGC 
CAGTCTTTGA AAGCTCACTT GGGAGCTGAA AGAGGTGACA GTGAAAAGAA GAGCCATGAA 
GACGAAGCTG TGTTCCATTC AGTTCAGAAC ACTGAATACT TTGAACACAA TGACAATGAT 
TATGATATAG ATTTTGTTCC ACCTTCTCCA GAAGAAATCA TCTCCACTGC TTCTTCCTCG 
TTGAAATGTT CCAGTATGTT AAAGGATCTT GATGACTCTG ACAAAGAAAA GGGCATTCTT 
AGCACCTCAG AAGAGCTTCT GTCAAAACCA GAGGAAATGA CCACACACAA GTCTGATGCA 
GGAACCAGTA AAGACTGTGA TGCCCAGCAG ATACGCATAC AGCAGCAGCT TATTCATGTG 
ATGGAGCACA TCTGTAAGTT AGTTGATACT GTTCCTACTG ATGAACTGGA AGCTTTGAAT 
TGTGGGACCG AATTGCTTCA ACAACGAAAC ATAAGGAGGA AGCTCCTAGC TGAAGCAGGT 
TTTAATGGAA ATGACGTCAG ACTTCTGGGT TCTCTGTGGA GGCACAGGCC TGATTCACTT 
GATAACACAG TGCAGGGCGA CTCCTGCCCT GTGGGGCATC CTAATAAAGA GTTAAATTCT 
CCATACCTTC TCTCACATTC CCCTTCCACT GAGGAATGTT TACCCACCAC CACTCCAGGA 
AAGACAGGAT TCTCAGCCAC CCCGAAGAAT CTCTTTGAAA GGCCGTTATT GAATTCCCAT 
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGGATGGA AAACAGGAAC 
GAAAGCACTG ACTTCCCAGG GAGTGTTCTC ACCAGCACCA CTGTGAAAGC TCAGAGTAAA 
CAAGCTGCTT CAGGATGGAA CGTAGAGAGA CACGGCCAGG CTTCCTATGA TATCGATAAC 
TTTAATATTG ATGACTTTGA TGATGATGAT GATGATGATG ACTGGGAAAA CATAATGCAC 
AATTTTCCAG CCAGCAAATC TTCCACAGCC ACCTACCCAC CCATCAAGGA AGGTGGGCCA 
GTTAAATCTC TCTCAGAAAG GATTTCTTCA GCCAAGGCAA AGTTTCTTCC AGTGGTATCA 
ACCGCTCAAA ATACAAACCT CTCAGAGTCA ATTCAGAATT GCTCTGATAA GCTGGCCCAA 
AATTTATCAT CAAAAAATCC AAAACATGAA CATTTTCAAA GTCTTAATTT TCCTCATACA 
AAAGAAATGA TGAAGATTTT CCATAAGAAA TTTGGCTTGC ATAATTTTAG AACTAATCAG 
CTAGAGGCGA TCAATGCTGC GCTGCTTGGT GAAGACTGCT TTATCCTAAT GCCCACTGGA 
GGAGGTAAAA GTTTGTGCTA CCAGCTCCCT GCCTGTGTTT CTCCTGGGGT CACAATTGTC 
ATTTCTCCCT TGAGATCACT AATAGTAGAT CAAGTCCAAA AGCTGACTTC CTTTGATATT 
CCAGCTACAT ATCTGACAGG GGATAAGACT GACTCAGAAG CTGCAAATAT TTACCTCCAA 
TTATCCAAAA AAGACCCAAT CATCAAGCTT TTGTATGTTA CTCCAGAGAA GGTCTGTGCA 
AGTAACAGGC TGATTTCTAC TCTGGAGAAT CTGTATGAGC GGAAGCTCTT GGCACGTTTT 
GTCATTGATG AAGCGCATTG TGTGAGTCAG TGGGGTCATG ATTTTCGTCA AGATTACAAA 
AGGATGAATA TGCTTCGCCA GAAGTTTCCT TCTGTTCCAG TGATGGCCCT CACGGCCACA 
GCGAACCCCA GGGTCCAGAA GGACATCCTC ACTCAGCTGA AGATCCTCAG ACCTCAGGTG 
TTTAGCATGA GCTTTAACAG ACACAATCTG AAGTACTATG TATTACCCAA GAAGCCCAAA 
AAAGTAGCAT TTGATTGCCT AGAGTGGATC AGAAAGCATC ACCCTTATGA CTCGGGGATA 
ATTTACTGCC TCTCCAGGAG GGAATGTGAC ACAATGGCTG ACACTTTACA GAGAGAAGGC 
CTGGCTGCCC TGGCTTACCA TGCGGGCCTC AGTGACTCTG CCAGAGATGA GGTGCAGCAC 
AAGTGGATCA ACCAGGACAA CTGCCAGTGG AAAAAGATGG AAACTATCAT ACGAAGGAAA 
CTCACGTCAA TAATCTATAT AGCATGGTAC ATTACTGTGA AAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blm ( XM_017321957.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177446.1 Mus musculus Bloom syndrome, RecQ helicase-like (Blm), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu81913
Clone ID Related Accession (Same CDS sequence)	XM_017321957.1 , XM_017321957.2
Accession Version	XM_017321957.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	Bloom syndrome protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG TTCCTCTGAA CAATCTACAA GAACAACTAC AGCGACACTC AGCCAGAAAA 
CTTAATAATC AACCCAGCCT TTCAAAACCA AAATCTTTAG GTTTTACTTT TAAAAAGAAA 
ACATCAGAGG GTGATGTGTC TGTCACTAGT GTGTCCGTAG TAAAAACACC TGCGTTAAGT 
GATAAAGATG TGAACGTGTC TGAGGCCTTT TCATTCACTG AGTCTCCACT CCACAAACCA 
AAGCAGCAGG CAAAGATTGA AGGCTTCTTT AAACATTTCC CTGGAAGGCA GCAAAGCAAG 
GGGACCTGCT CTGAGCCGTC ACTGCCGGCC ACGGTACAGA CTGCTCAGGA CACTTTGTGC 
ACTACCCCCA AAACCCCCAC TGCGAAGAAA CTGCCCGTGG CTGTTTTCAA GAAATTAGAA 
TTTAGTTCTT CTGCAGACTC CCTCAGTGAC TGGGCTGATA TGGATGACTT TGATATGTCA 
GCATCAGATG CGTTTGCTTC ACTGGCTAAA AATCCTGCCA CAAGAGTAAG CACCGCTCAG 
AAAATGAAAA AGACTAAGAG AAACTTCTTT AAACCACCAC CTCGTAAAGC CAATGCAGTA 
AAGACTGACT TGACTCCTCC CTCCCCCGAA TGCCTGCAAG TGGATTTAAC GAAGGAATCG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGCG GAGGGGGCGG ACTGCCTGAG CAGGGATGTG 
ATCTGCATTG ACAATGATTC TGCTTCTGAA GAGCTCACGG AGAAAGACAC GCAGGAAAGC 
CAGTCTTTGA AAGCTCACTT GGGAGCTGAA AGAGGTGACA GTGAAAAGAA GAGCCATGAA 
GACGAAGCTG TGTTCCATTC AGTTCAGAAC ACTGAATACT TTGAACACAA TGACAATGAT 
TATGATATAG ATTTTGTTCC ACCTTCTCCA GAAGAAATCA TCTCCACTGC TTCTTCCTCG 
TTGAAATGTT CCAGTATGTT AAAGGATCTT GATGACTCTG ACAAAGAAAA GGGCATTCTT 
AGCACCTCAG AAGAGCTTCT GTCAAAACCA GAGGAAATGA CCACACACAA GTCTGATGCA 
GGAACCAGTA AAGACTGTGA TGCCCAGCAG ATACGCATAC AGCAGCAGCT TATTCATGTG 
ATGGAGCACA TCTGTAAGTT AGTTGATACT GTTCCTACTG ATGAACTGGA AGCTTTGAAT 
TGTGGGACCG AATTGCTTCA ACAACGAAAC ATAAGGAGGA AGCTCCTAGC TGAAGCAGGT 
TTTAATGGAA ATGACGTCAG ACTTCTGGGT TCTCTGTGGA GGCACAGGCC TGATTCACTT 
GATAACACAG TGCAGGGCGA CTCCTGCCCT GTGGGGCATC CTAATAAAGA GTTAAATTCT 
CCATACCTTC TCTCACATTC CCCTTCCACT GAGGAATGTT TACCCACCAC CACTCCAGGA 
AAGACAGGAT TCTCAGCCAC CCCGAAGAAT CTCTTTGAAA GGCCGTTATT GAATTCCCAT 
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGGATGGA AAACAGGAAC 
GAAAGCACTG ACTTCCCAGG GAGTGTTCTC ACCAGCACCA CTGTGAAAGC TCAGAGTAAA 
CAAGCTGCTT CAGGATGGAA CGTAGAGAGA CACGGCCAGG CTTCCTATGA TATCGATAAC 
TTTAATATTG ATGACTTTGA TGATGATGAT GATGATGATG ACTGGGAAAA CATAATGCAC 
AATTTTCCAG CCAGCAAATC TTCCACAGCC ACCTACCCAC CCATCAAGGA AGGTGGGCCA 
GTTAAATCTC TCTCAGAAAG GATTTCTTCA GCCAAGGCAA AGTTTCTTCC AGTGGTATCA 
ACCGCTCAAA ATACAAACCT CTCAGAGTCA ATTCAGAATT GCTCTGATAA GCTGGCCCAA 
AATTTATCAT CAAAAAATCC AAAACATGAA CATTTTCAAA GTCTTAATTT TCCTCATACA 
AAAGAAATGA TGAAGATTTT CCATAAGAAA TTTGGCTTGC ATAATTTTAG AACTAATCAG 
CTAGAGGCGA TCAATGCTGC GCTGCTTGGT GAAGACTGCT TTATCCTAAT GCCCACTGGA 
GGAGGTAAAA GTTTGTGCTA CCAGCTCCCT GCCTGTGTTT CTCCTGGGGT CACAATTGTC 
ATTTCTCCCT TGAGATCACT AATAGTAGAT CAAGTCCAAA AGCTGACTTC CTTTGATATT 
CCAGCTACAT ATCTGACAGG GGATAAGACT GACTCAGAAG CTGCAAATAT TTACCTCCAA 
TTATCCAAAA AAGACCCAAT CATCAAGCTT TTGTATGTTA CTCCAGAGAA GGTCTGTGCA 
AGTAACAGGC TGATTTCTAC TCTGGAGAAT CTGTATGAGC GGAAGCTCTT GGCACGTTTT 
GTCATTGATG AAGCGCATTG TGTGAGTCAG TGGGGTCATG ATTTTCGTCA AGATTACAAA 
AGGATGAATA TGCTTCGCCA GAAGTTTCCT TCTGTTCCAG TGATGGCCCT CACGGCCACA 
GCGAACCCCA GGGTCCAGAA GGACATCCTC ACTCAGCTGA AGATCCTCAG ACCTCAGGTA 
TTACATTTTG TGTATCTTAC TTTGGCTTCC ACACTCCACG TGCCTTCATG GAATGAGCAG 
GTAACCCTGC TTTCTCATCT TAAAGTCTCT AGTCCATGCA CGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177446.1
CDS	110..2797
Translation	MAAVPLNNLQEQLQRHSARKLNNQPSLSKPKSLGFTFKKKTSEGDVSVTSVSVVKTPALSDKDVNVSEAFSFTESPLHKPKQQAKIEGFFKHFPGRQQSKGTCSEPSLPATVQTAQDTLCTTPKTPTAKKLPVAVFKKLEFSSSADSLSDWADMDDFDMSASDAFASLAKNPATRVSTAQKMKKTKRNFFKPPPRKANAVKTDLTPPSPECLQVDLTKESEEEEEEEEEAEGADCLSRDVICIDNDSASEELTEKDTQESQSLKAHLGAERGDSEKKSHEDEAVFHSVQNTEYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKGILSTSEELLSKPEEMTTHKSDAGTSKDCDAQQIRIQQQLIHVMEHICKLVDTVPTDELEALNCGTELLQQRNIRRKLLAEAGFNGNDVRLLGSLWRHRPDSLDNTVQGDSCPVGHPNKELNSPYLLSHSPSTEECLPTTTPGKTGFSATPKNLFERPLLNSHLQKSFVSSNWAETPRMENRNESTDFPGSVLTSTTVKAQSKQAASGWNVERHGQASYDIDNFNIDDFDDDDDDDDWENIMHNFPASKSSTATYPPIKEGGPVKSLSERISSAKAKFLPVVSTAQNTNLSESIQNCSDKLAQNLSSKNPKHEHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVLHFVYLTLASTLHVPSWNEQVTLLSHLKVSSPCTI

Target ORF information:

RefSeq Version	XM_017321957.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bloom syndrome, RecQ helicase-like (Blm), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321957.1

ORF Insert Sequence:

ATGGCTGCTG TTCCTCTGAA CAATCTACAA GAACAACTAC AGCGACACTC AGCCAGAAAA 
CTTAATAATC AACCCAGCCT TTCAAAACCA AAATCTTTAG GTTTTACTTT TAAAAAGAAA 
ACATCAGAGG GTGATGTGTC TGTCACTAGT GTGTCCGTAG TAAAAACACC TGCGTTAAGT 
GATAAAGATG TGAACGTGTC TGAGGCCTTT TCATTCACTG AGTCTCCACT CCACAAACCA 
AAGCAGCAGG CAAAGATTGA AGGCTTCTTT AAACATTTCC CTGGAAGGCA GCAAAGCAAG 
GGGACCTGCT CTGAGCCGTC ACTGCCGGCC ACGGTACAGA CTGCTCAGGA CACTTTGTGC 
ACTACCCCCA AAACCCCCAC TGCGAAGAAA CTGCCCGTGG CTGTTTTCAA GAAATTAGAA 
TTTAGTTCTT CTGCAGACTC CCTCAGTGAC TGGGCTGATA TGGATGACTT TGATATGTCA 
GCATCAGATG CGTTTGCTTC ACTGGCTAAA AATCCTGCCA CAAGAGTAAG CACCGCTCAG 
AAAATGAAAA AGACTAAGAG AAACTTCTTT AAACCACCAC CTCGTAAAGC CAATGCAGTA 
AAGACTGACT TGACTCCTCC CTCCCCCGAA TGCCTGCAAG TGGATTTAAC GAAGGAATCG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGCG GAGGGGGCGG ACTGCCTGAG CAGGGATGTG 
ATCTGCATTG ACAATGATTC TGCTTCTGAA GAGCTCACGG AGAAAGACAC GCAGGAAAGC 
CAGTCTTTGA AAGCTCACTT GGGAGCTGAA AGAGGTGACA GTGAAAAGAA GAGCCATGAA 
GACGAAGCTG TGTTCCATTC AGTTCAGAAC ACTGAATACT TTGAACACAA TGACAATGAT 
TATGATATAG ATTTTGTTCC ACCTTCTCCA GAAGAAATCA TCTCCACTGC TTCTTCCTCG 
TTGAAATGTT CCAGTATGTT AAAGGATCTT GATGACTCTG ACAAAGAAAA GGGCATTCTT 
AGCACCTCAG AAGAGCTTCT GTCAAAACCA GAGGAAATGA CCACACACAA GTCTGATGCA 
GGAACCAGTA AAGACTGTGA TGCCCAGCAG ATACGCATAC AGCAGCAGCT TATTCATGTG 
ATGGAGCACA TCTGTAAGTT AGTTGATACT GTTCCTACTG ATGAACTGGA AGCTTTGAAT 
TGTGGGACCG AATTGCTTCA ACAACGAAAC ATAAGGAGGA AGCTCCTAGC TGAAGCAGGT 
TTTAATGGAA ATGACGTCAG ACTTCTGGGT TCTCTGTGGA GGCACAGGCC TGATTCACTT 
GATAACACAG TGCAGGGCGA CTCCTGCCCT GTGGGGCATC CTAATAAAGA GTTAAATTCT 
CCATACCTTC TCTCACATTC CCCTTCCACT GAGGAATGTT TACCCACCAC CACTCCAGGA 
AAGACAGGAT TCTCAGCCAC CCCGAAGAAT CTCTTTGAAA GGCCGTTATT GAATTCCCAT 
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGGATGGA AAACAGGAAC 
GAAAGCACTG ACTTCCCAGG GAGTGTTCTC ACCAGCACCA CTGTGAAAGC TCAGAGTAAA 
CAAGCTGCTT CAGGATGGAA CGTAGAGAGA CACGGCCAGG CTTCCTATGA TATCGATAAC 
TTTAATATTG ATGACTTTGA TGATGATGAT GATGATGATG ACTGGGAAAA CATAATGCAC 
AATTTTCCAG CCAGCAAATC TTCCACAGCC ACCTACCCAC CCATCAAGGA AGGTGGGCCA 
GTTAAATCTC TCTCAGAAAG GATTTCTTCA GCCAAGGCAA AGTTTCTTCC AGTGGTATCA 
ACCGCTCAAA ATACAAACCT CTCAGAGTCA ATTCAGAATT GCTCTGATAA GCTGGCCCAA 
AATTTATCAT CAAAAAATCC AAAACATGAA CATTTTCAAA GTCTTAATTT TCCTCATACA 
AAAGAAATGA TGAAGATTTT CCATAAGAAA TTTGGCTTGC ATAATTTTAG AACTAATCAG 
CTAGAGGCGA TCAATGCTGC GCTGCTTGGT GAAGACTGCT TTATCCTAAT GCCCACTGGA 
GGAGGTAAAA GTTTGTGCTA CCAGCTCCCT GCCTGTGTTT CTCCTGGGGT CACAATTGTC 
ATTTCTCCCT TGAGATCACT AATAGTAGAT CAAGTCCAAA AGCTGACTTC CTTTGATATT 
CCAGCTACAT ATCTGACAGG GGATAAGACT GACTCAGAAG CTGCAAATAT TTACCTCCAA 
TTATCCAAAA AAGACCCAAT CATCAAGCTT TTGTATGTTA CTCCAGAGAA GGTCTGTGCA 
AGTAACAGGC TGATTTCTAC TCTGGAGAAT CTGTATGAGC GGAAGCTCTT GGCACGTTTT 
GTCATTGATG AAGCGCATTG TGTGAGTCAG TGGGGTCATG ATTTTCGTCA AGATTACAAA 
AGGATGAATA TGCTTCGCCA GAAGTTTCCT TCTGTTCCAG TGATGGCCCT CACGGCCACA 
GCGAACCCCA GGGTCCAGAA GGACATCCTC ACTCAGCTGA AGATCCTCAG ACCTCAGGTA 
TTACATTTTG TGTATCTTAC TTTGGCTTCC ACACTCCACG TGCCTTCATG GAATGAGCAG 
GTAACCCTGC TTTCTCATCT TAAAGTCTCT AGTCCATGCA CGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blm ( XM_017321957.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177446.1 Mus musculus Bloom syndrome, RecQ like helicase (Blm), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu81913
Clone ID Related Accession (Same CDS sequence)	XM_017321957.1 , XM_017321957.2
Accession Version	XM_017321957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	Bloom syndrome protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG TTCCTCTGAA CAATCTACAA GAACAACTAC AGCGACACTC AGCCAGAAAA 
CTTAATAATC AACCCAGCCT TTCAAAACCA AAATCTTTAG GTTTTACTTT TAAAAAGAAA 
ACATCAGAGG GTGATGTGTC TGTCACTAGT GTGTCCGTAG TAAAAACACC TGCGTTAAGT 
GATAAAGATG TGAACGTGTC TGAGGCCTTT TCATTCACTG AGTCTCCACT CCACAAACCA 
AAGCAGCAGG CAAAGATTGA AGGCTTCTTT AAACATTTCC CTGGAAGGCA GCAAAGCAAG 
GGGACCTGCT CTGAGCCGTC ACTGCCGGCC ACGGTACAGA CTGCTCAGGA CACTTTGTGC 
ACTACCCCCA AAACCCCCAC TGCGAAGAAA CTGCCCGTGG CTGTTTTCAA GAAATTAGAA 
TTTAGTTCTT CTGCAGACTC CCTCAGTGAC TGGGCTGATA TGGATGACTT TGATATGTCA 
GCATCAGATG CGTTTGCTTC ACTGGCTAAA AATCCTGCCA CAAGAGTAAG CACCGCTCAG 
AAAATGAAAA AGACTAAGAG AAACTTCTTT AAACCACCAC CTCGTAAAGC CAATGCAGTA 
AAGACTGACT TGACTCCTCC CTCCCCCGAA TGCCTGCAAG TGGATTTAAC GAAGGAATCG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGCG GAGGGGGCGG ACTGCCTGAG CAGGGATGTG 
ATCTGCATTG ACAATGATTC TGCTTCTGAA GAGCTCACGG AGAAAGACAC GCAGGAAAGC 
CAGTCTTTGA AAGCTCACTT GGGAGCTGAA AGAGGTGACA GTGAAAAGAA GAGCCATGAA 
GACGAAGCTG TGTTCCATTC AGTTCAGAAC ACTGAATACT TTGAACACAA TGACAATGAT 
TATGATATAG ATTTTGTTCC ACCTTCTCCA GAAGAAATCA TCTCCACTGC TTCTTCCTCG 
TTGAAATGTT CCAGTATGTT AAAGGATCTT GATGACTCTG ACAAAGAAAA GGGCATTCTT 
AGCACCTCAG AAGAGCTTCT GTCAAAACCA GAGGAAATGA CCACACACAA GTCTGATGCA 
GGAACCAGTA AAGACTGTGA TGCCCAGCAG ATACGCATAC AGCAGCAGCT TATTCATGTG 
ATGGAGCACA TCTGTAAGTT AGTTGATACT GTTCCTACTG ATGAACTGGA AGCTTTGAAT 
TGTGGGACCG AATTGCTTCA ACAACGAAAC ATAAGGAGGA AGCTCCTAGC TGAAGCAGGT 
TTTAATGGAA ATGACGTCAG ACTTCTGGGT TCTCTGTGGA GGCACAGGCC TGATTCACTT 
GATAACACAG TGCAGGGCGA CTCCTGCCCT GTGGGGCATC CTAATAAAGA GTTAAATTCT 
CCATACCTTC TCTCACATTC CCCTTCCACT GAGGAATGTT TACCCACCAC CACTCCAGGA 
AAGACAGGAT TCTCAGCCAC CCCGAAGAAT CTCTTTGAAA GGCCGTTATT GAATTCCCAT 
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGGATGGA AAACAGGAAC 
GAAAGCACTG ACTTCCCAGG GAGTGTTCTC ACCAGCACCA CTGTGAAAGC TCAGAGTAAA 
CAAGCTGCTT CAGGATGGAA CGTAGAGAGA CACGGCCAGG CTTCCTATGA TATCGATAAC 
TTTAATATTG ATGACTTTGA TGATGATGAT GATGATGATG ACTGGGAAAA CATAATGCAC 
AATTTTCCAG CCAGCAAATC TTCCACAGCC ACCTACCCAC CCATCAAGGA AGGTGGGCCA 
GTTAAATCTC TCTCAGAAAG GATTTCTTCA GCCAAGGCAA AGTTTCTTCC AGTGGTATCA 
ACCGCTCAAA ATACAAACCT CTCAGAGTCA ATTCAGAATT GCTCTGATAA GCTGGCCCAA 
AATTTATCAT CAAAAAATCC AAAACATGAA CATTTTCAAA GTCTTAATTT TCCTCATACA 
AAAGAAATGA TGAAGATTTT CCATAAGAAA TTTGGCTTGC ATAATTTTAG AACTAATCAG 
CTAGAGGCGA TCAATGCTGC GCTGCTTGGT GAAGACTGCT TTATCCTAAT GCCCACTGGA 
GGAGGTAAAA GTTTGTGCTA CCAGCTCCCT GCCTGTGTTT CTCCTGGGGT CACAATTGTC 
ATTTCTCCCT TGAGATCACT AATAGTAGAT CAAGTCCAAA AGCTGACTTC CTTTGATATT 
CCAGCTACAT ATCTGACAGG GGATAAGACT GACTCAGAAG CTGCAAATAT TTACCTCCAA 
TTATCCAAAA AAGACCCAAT CATCAAGCTT TTGTATGTTA CTCCAGAGAA GGTCTGTGCA 
AGTAACAGGC TGATTTCTAC TCTGGAGAAT CTGTATGAGC GGAAGCTCTT GGCACGTTTT 
GTCATTGATG AAGCGCATTG TGTGAGTCAG TGGGGTCATG ATTTTCGTCA AGATTACAAA 
AGGATGAATA TGCTTCGCCA GAAGTTTCCT TCTGTTCCAG TGATGGCCCT CACGGCCACA 
GCGAACCCCA GGGTCCAGAA GGACATCCTC ACTCAGCTGA AGATCCTCAG ACCTCAGGTA 
TTACATTTTG TGTATCTTAC TTTGGCTTCC ACACTCCACG TGCCTTCATG GAATGAGCAG 
GTAACCCTGC TTTCTCATCT TAAAGTCTCT AGTCCATGCA CGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177446.1
CDS	111..2798
Translation	MAAVPLNNLQEQLQRHSARKLNNQPSLSKPKSLGFTFKKKTSEGDVSVTSVSVVKTPALSDKDVNVSEAFSFTESPLHKPKQQAKIEGFFKHFPGRQQSKGTCSEPSLPATVQTAQDTLCTTPKTPTAKKLPVAVFKKLEFSSSADSLSDWADMDDFDMSASDAFASLAKNPATRVSTAQKMKKTKRNFFKPPPRKANAVKTDLTPPSPECLQVDLTKESEEEEEEEEEAEGADCLSRDVICIDNDSASEELTEKDTQESQSLKAHLGAERGDSEKKSHEDEAVFHSVQNTEYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKGILSTSEELLSKPEEMTTHKSDAGTSKDCDAQQIRIQQQLIHVMEHICKLVDTVPTDELEALNCGTELLQQRNIRRKLLAEAGFNGNDVRLLGSLWRHRPDSLDNTVQGDSCPVGHPNKELNSPYLLSHSPSTEECLPTTTPGKTGFSATPKNLFERPLLNSHLQKSFVSSNWAETPRMENRNESTDFPGSVLTSTTVKAQSKQAASGWNVERHGQASYDIDNFNIDDFDDDDDDDDWENIMHNFPASKSSTATYPPIKEGGPVKSLSERISSAKAKFLPVVSTAQNTNLSESIQNCSDKLAQNLSSKNPKHEHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVLHFVYLTLASTLHVPSWNEQVTLLSHLKVSSPCTI

Target ORF information:

RefSeq Version	XM_017321957.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bloom syndrome, RecQ like helicase (Blm), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321957.2

ORF Insert Sequence:

ATGGCTGCTG TTCCTCTGAA CAATCTACAA GAACAACTAC AGCGACACTC AGCCAGAAAA 
CTTAATAATC AACCCAGCCT TTCAAAACCA AAATCTTTAG GTTTTACTTT TAAAAAGAAA 
ACATCAGAGG GTGATGTGTC TGTCACTAGT GTGTCCGTAG TAAAAACACC TGCGTTAAGT 
GATAAAGATG TGAACGTGTC TGAGGCCTTT TCATTCACTG AGTCTCCACT CCACAAACCA 
AAGCAGCAGG CAAAGATTGA AGGCTTCTTT AAACATTTCC CTGGAAGGCA GCAAAGCAAG 
GGGACCTGCT CTGAGCCGTC ACTGCCGGCC ACGGTACAGA CTGCTCAGGA CACTTTGTGC 
ACTACCCCCA AAACCCCCAC TGCGAAGAAA CTGCCCGTGG CTGTTTTCAA GAAATTAGAA 
TTTAGTTCTT CTGCAGACTC CCTCAGTGAC TGGGCTGATA TGGATGACTT TGATATGTCA 
GCATCAGATG CGTTTGCTTC ACTGGCTAAA AATCCTGCCA CAAGAGTAAG CACCGCTCAG 
AAAATGAAAA AGACTAAGAG AAACTTCTTT AAACCACCAC CTCGTAAAGC CAATGCAGTA 
AAGACTGACT TGACTCCTCC CTCCCCCGAA TGCCTGCAAG TGGATTTAAC GAAGGAATCG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGCG GAGGGGGCGG ACTGCCTGAG CAGGGATGTG 
ATCTGCATTG ACAATGATTC TGCTTCTGAA GAGCTCACGG AGAAAGACAC GCAGGAAAGC 
CAGTCTTTGA AAGCTCACTT GGGAGCTGAA AGAGGTGACA GTGAAAAGAA GAGCCATGAA 
GACGAAGCTG TGTTCCATTC AGTTCAGAAC ACTGAATACT TTGAACACAA TGACAATGAT 
TATGATATAG ATTTTGTTCC ACCTTCTCCA GAAGAAATCA TCTCCACTGC TTCTTCCTCG 
TTGAAATGTT CCAGTATGTT AAAGGATCTT GATGACTCTG ACAAAGAAAA GGGCATTCTT 
AGCACCTCAG AAGAGCTTCT GTCAAAACCA GAGGAAATGA CCACACACAA GTCTGATGCA 
GGAACCAGTA AAGACTGTGA TGCCCAGCAG ATACGCATAC AGCAGCAGCT TATTCATGTG 
ATGGAGCACA TCTGTAAGTT AGTTGATACT GTTCCTACTG ATGAACTGGA AGCTTTGAAT 
TGTGGGACCG AATTGCTTCA ACAACGAAAC ATAAGGAGGA AGCTCCTAGC TGAAGCAGGT 
TTTAATGGAA ATGACGTCAG ACTTCTGGGT TCTCTGTGGA GGCACAGGCC TGATTCACTT 
GATAACACAG TGCAGGGCGA CTCCTGCCCT GTGGGGCATC CTAATAAAGA GTTAAATTCT 
CCATACCTTC TCTCACATTC CCCTTCCACT GAGGAATGTT TACCCACCAC CACTCCAGGA 
AAGACAGGAT TCTCAGCCAC CCCGAAGAAT CTCTTTGAAA GGCCGTTATT GAATTCCCAT 
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGGATGGA AAACAGGAAC 
GAAAGCACTG ACTTCCCAGG GAGTGTTCTC ACCAGCACCA CTGTGAAAGC TCAGAGTAAA 
CAAGCTGCTT CAGGATGGAA CGTAGAGAGA CACGGCCAGG CTTCCTATGA TATCGATAAC 
TTTAATATTG ATGACTTTGA TGATGATGAT GATGATGATG ACTGGGAAAA CATAATGCAC 
AATTTTCCAG CCAGCAAATC TTCCACAGCC ACCTACCCAC CCATCAAGGA AGGTGGGCCA 
GTTAAATCTC TCTCAGAAAG GATTTCTTCA GCCAAGGCAA AGTTTCTTCC AGTGGTATCA 
ACCGCTCAAA ATACAAACCT CTCAGAGTCA ATTCAGAATT GCTCTGATAA GCTGGCCCAA 
AATTTATCAT CAAAAAATCC AAAACATGAA CATTTTCAAA GTCTTAATTT TCCTCATACA 
AAAGAAATGA TGAAGATTTT CCATAAGAAA TTTGGCTTGC ATAATTTTAG AACTAATCAG 
CTAGAGGCGA TCAATGCTGC GCTGCTTGGT GAAGACTGCT TTATCCTAAT GCCCACTGGA 
GGAGGTAAAA GTTTGTGCTA CCAGCTCCCT GCCTGTGTTT CTCCTGGGGT CACAATTGTC 
ATTTCTCCCT TGAGATCACT AATAGTAGAT CAAGTCCAAA AGCTGACTTC CTTTGATATT 
CCAGCTACAT ATCTGACAGG GGATAAGACT GACTCAGAAG CTGCAAATAT TTACCTCCAA 
TTATCCAAAA AAGACCCAAT CATCAAGCTT TTGTATGTTA CTCCAGAGAA GGTCTGTGCA 
AGTAACAGGC TGATTTCTAC TCTGGAGAAT CTGTATGAGC GGAAGCTCTT GGCACGTTTT 
GTCATTGATG AAGCGCATTG TGTGAGTCAG TGGGGTCATG ATTTTCGTCA AGATTACAAA 
AGGATGAATA TGCTTCGCCA GAAGTTTCCT TCTGTTCCAG TGATGGCCCT CACGGCCACA 
GCGAACCCCA GGGTCCAGAA GGACATCCTC ACTCAGCTGA AGATCCTCAG ACCTCAGGTA 
TTACATTTTG TGTATCTTAC TTTGGCTTCC ACACTCCACG TGCCTTCATG GAATGAGCAG 
GTAACCCTGC TTTCTCATCT TAAAGTCTCT AGTCCATGCA CGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blm ( NM_001042527.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001035992.1 Mus musculus Bloom syndrome, RecQ like helicase (Blm), transcript variant 2, mRNA.

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CloneID	OMu15901
Clone ID Related Accession (Same CDS sequence)	NM_001042527.2
Accession Version	NM_001042527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	Bloom syndrome protein homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK157898.1, Z98263.1 and AC110907.7. On Apr 15, 2009 this sequence version replaced NM_001042527.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream translational start codon, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z98263.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCTG TTCCTCTGAA CAATCTACAA GAACAACTAC AGCGACACTC AGCCAGAAAA 
CTTAATAATC AACCCAGCCT TTCAAAACCA AAATCTTTAG GTTTTACTTT TAAAAAGAAA 
ACATCAGAGG GTGATGTGTC TGTCACTAGT GTGTCCGTAG TAAAAACACC TGCGTTAAGT 
GATAAAGATG TGAACGTGTC TGAGGCCTTT TCATTCACTG AGTCTCCACT CCACAAACCA 
AAGCAGCAGG CAAAGATTGA AGGCTTCTTT AAACATTTCC CTGGAAGGCA GCAAAGCAAG 
GGGACCTGCT CTGAGCCGTC ACTGCCGGCC ACGGTACAGA CTGCTCAGGA CACTTTGTGC 
ACTACCCCCA AAACCCCCAC TGCGAAGAAA CTGCCCGTGG CTGTTTTCAA GAAATTAGAA 
TTTAGTTCTT CTGCAGACTC CCTCAGTGAC TGGGCTGATA TGGATGACTT TGATATGTCA 
GCATCAGATG CGTTTGCTTC ACTGGCTAAA AATCCTGCCA CAAGAGTAAG CACCGCTCAG 
AAAATGAAAA AGACTAAGAG AAACTTCTTT AAACCACCAC CTCGTAAAGC CAATGCAGTA 
AAGACTGACT TGACTCCTCC CTCCCCCGAA TGCCTGCAAG TGGATTTAAC GAAGGAATCG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGCG GAGGGGGCGG ACTGCCTGAG CAGGGATGTG 
ATCTGCATTG ACAATGATTC TGCTTCTGAA GAGCTCACGG AGAAAGACAC GCAGGAAAGC 
CAGTCTTTGA AAGCTCACTT GGGAGCTGAA AGAGGTGACA GTGAAAAGAA GAGCCATGAA 
GACGAAGCTG TGTTCCATTC AGTTCAGAAC ACTGAATACT TTGAACACAA TGACAATGAT 
TATGATATAG ATTTTGTTCC ACCTTCTCCA GAAGAAATCA TCTCCACTGC TTCTTCCTCG 
TTGAAATGTT CCAGTATGTT AAAGGATCTT GATGACTCTG ACAAAGAAAA GGGCATTCTT 
AGCACCTCAG AAGAGCTTCT GTCAAAACCA GAGGAAATGA CCACACACAA GTCTGATGCA 
GGAACCAGTA AAGACTGTGA TGCCCAGCAG ATACGCATAC AGCAGCAGCT TATTCATGTG 
ATGGAGCACA TCTGTAAGTT AGTTGATACT GTTCCTACTG ATGAACTGGA AGCTTTGAAT 
TGTGGGACCG AATTGCTTCA ACAACGAAAC ATAAGGAGGA AGCTCCTAGC TGAAGCAGGT 
TTTAATGGAA ATGACGTCAG ACTTCTGGGT TCTCTGTGGA GGCACAGGCC TGATTCACTT 
GATAACACAG TGCAGGGCGA CTCCTGCCCT GTGGGGCATC CTAATAAAGA GTTAAATTCT 
CCATACCTTC TCTCACATTC CCCTTCCACT GAGGAATGTT TACCCACCAC CACTCCAGGA 
AAGACAGGAT TCTCAGCCAC CCCGAAGAAT CTCTTTGAAA GGCCGTTATT GAATTCCCAT 
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGGATGGA AAACAGGAAC 
GAAAGCACTG ACTTCCCAGG GAGTGTTCTC ACCAGCACCA CTGTGAAAGC TCAGAGTAAA 
CAAGCTGCTT CAGGATGGAA CGTAGAGAGA CACGGCCAGG CTTCCTATGA TATCGATAAC 
TTTAATATTG ATGACTTTGA TGATGATGAT GATGATGATG ACTGGGAAAA CATAATGCAC 
AATTTTCCAG CCAGCAAATC TTCCACAGCC ACCTACCCAC CCATCAAGGA AGGTGGGCCA 
GTTAAATCTC TCTCAGAAAG GATTTCTTCA GCCAAGGCAA AGTTTCTTCC AGTGGTATCA 
ACCGCTCAAA ATACAAACCT CTCAGAGTCA ATTCAGAATT GCTCTGATAA GCTGGCCCAA 
AATTTATCAT CAAAAAATCC AAAACATGAA CATTTTCAAA GTCTTAATTT TCCTCATACA 
AAAGAAATGA TGAAGATTTT CCATAAGAAA TTTGGCTTGC ATAATTTTAG AACTAATCAG 
CTAGAGGCGA TCAATGCTGC GCTGCTTGGT GAAGACTGCT TTATCCTAAT GCCCACTGGA 
GGAGGTAAAA GTTTGTGCTA CCAGCTCCCT GCCTGTGTTT CTCCTGGGGT CACAATTGTC 
ATTTCTCCCT TGAGATCACT AATAGTAGAT CAAGTCCAAA AGCTGACTTC CTTTGATATT 
CCAGCTACAT ATCTGACAGG GGATAAGACT GACTCAGAAG CTGCAAATAT TTACCTCCAA 
TTATCCAAAA AAGACCCAAT CATCAAGCTT TTGTATGTTA CTCCAGAGAA GGTCTGTGCA 
AGTAACAGGC TGATTTCTAC TCTGGAGAAT CTGTATGAGC GGAAGCTCTT GGCACGTTTT 
GTCATTGATG AAGCGCATTG TGTGAGTCAG TGGGGTCATG ATTTTCGTCA AGATTACAAA 
AGGATGAATA TGCTTCGCCA GAAGTTTCCT TCTGTTCCAG TGATGGCCCT CACGGCCACA 
GCGAACCCCA GGGTCCAGAA GGACATCCTC ACTCAGCTGA AGATCCTCAG ACCTCAGGTG 
TTTAGCATGA GCTTTAACAG ACACAATCTG AAGTACTATG TATTACCCAA GAAGCCCAAA 
AAAGTAGCAT TTGATTGCCT AGAGTGGATC AGAAAGCATC ACCCTTATGA CTCGGGGATA 
ATTTACTGCC TCTCCAGGAG GGAATGTGAC ACAATGGCTG ACACTTTACA GAGAGAAGGC 
CTGGCTGCCC TGGCTTACCA TGCGGGCCTC AGTGACTCTG CCAGAGATGA GGTGCAGCAC 
AAGTGGATCA ACCAGGACAA CTGCCAGGTT ATCTGTGCGA CAATTGCGTT TGGAATGGGA 
ATTGACAAAC CTGACGTGCG ATTTGTGATT CATGCATCTC TTCCTAAATC TATGGAGGGT 
TATTATCAAG AATCCGGCCG AGCTGGAAGA GATGGGGAAA TATCTCACTG CGTGCTTTTC 
TATACATATC ATGATGTGAC CAGACTGAAG AGACTTATAA TGATGGAAAA AGATGGAAAC 
TATCATACGA AGGAAACTCA CGTCAATAAT CTATATAGCA TGGTACATTA CTGTGAAAAC 
ATAACGGAAT GCAGAAGAAT ACAGCTTTTA GCTTACTTTG GTGAAAAAGG ATTCAACCCT 
GATTTTTGTA AGAAATACCC AGATGTTTCT TGTGACAATT GCTGTAAAAC AAAGGATTAT 
AAAACAAAAG ATGTGACTGA TGACGTGAAA AATATTATAA GATTTGTTCA AGAACACAGT 
TCATCACCAG GAACAAGAAA TATAGGACCT GCTGGAAGAT TTACTCTGAA CATGCTGGTC 
GACATTTTCT TGGGGAGCAA GAGTGCAAAA GTTAAGTCTG GAATATTTGG AAAGGGGACT 
ACATATTCAC GACATAATGC CGAAAGACTT TTTAAAAAGC TGATTCTAGA CAAAATCCTG 
GATGAAGACT TATATATCAA TGCCAATGAC CAACCAATTG CCTATGTGAT GCTAGGAACA 
AAAGCCCACA GTGTGCTGAG TGGCCACTTG AAGGTGGACT TCATGGAAAC GGAAAATTCC 
AGCAGTATTA AAAAACAAAA AGCTTTAGTG GCCAAAGTAT CCCAGAGAGA AGAGGTAGTT 
AAGAAATGTC TTGGAGAACT TACAGAGGTC TGCAAATTGC TGGGGAAAGT CTTTGGTGTC 
CATTACTTCA ATATTTTTAA TACAGCCACA CTCAAAAAGC TTGCAGAATC TTTATCTTCT 
GATCCTGAGG TTTTGCTTCA GATTGATGGT GTTACCGAAG ACAAGCTGGA AAAATATGGT 
GCAGAAGTGA TTCCAGTATT ACAGAAGTAC TCAGAATGGA CAGTGCCAGC TGAGGATGGT 
TCCCCAGGCG CCAGAGGCGC CCCAGAGGAC ACTGAGGAGG AGGAGGAGGA AGCGCCTGTA 
TCTTCTCACT ACTTTGCAAA TCAAACTAGA AATGAAAGAA AGAGAAAGAA AATGTCAGCC 
ACCCATAAGC CCAAGAGGAG AAGAACTAGT TACGGTGGCT TCAGAGCAAA GGGGGGCTCT 
ACTACATGCA GAAAAACGAC TTCTAAAAGT AAATTCTATG GTGTAACTGG ATCCCGCTCG 
GCCTCATGTG CTTCTCAGGC AACATCATCA GCCAGTAGAA AACTGGGGAT TATGGCTCCT 
CCAAAGCCTG TAAATAGAAC GTTCCTCAGG CCTTCATATG CCTTCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035992.1
CDS	82..4332
Translation	MAAVPLNNLQEQLQRHSARKLNNQPSLSKPKSLGFTFKKKTSEGDVSVTSVSVVKTPALSDKDVNVSEAFSFTESPLHKPKQQAKIEGFFKHFPGRQQSKGTCSEPSLPATVQTAQDTLCTTPKTPTAKKLPVAVFKKLEFSSSADSLSDWADMDDFDMSASDAFASLAKNPATRVSTAQKMKKTKRNFFKPPPRKANAVKTDLTPPSPECLQVDLTKESEEEEEEEEEAEGADCLSRDVICIDNDSASEELTEKDTQESQSLKAHLGAERGDSEKKSHEDEAVFHSVQNTEYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKGILSTSEELLSKPEEMTTHKSDAGTSKDCDAQQIRIQQQLIHVMEHICKLVDTVPTDELEALNCGTELLQQRNIRRKLLAEAGFNGNDVRLLGSLWRHRPDSLDNTVQGDSCPVGHPNKELNSPYLLSHSPSTEECLPTTTPGKTGFSATPKNLFERPLLNSHLQKSFVSSNWAETPRMENRNESTDFPGSVLTSTTVKAQSKQAASGWNVERHGQASYDIDNFNIDDFDDDDDDDDWENIMHNFPASKSSTATYPPIKEGGPVKSLSERISSAKAKFLPVVSTAQNTNLSESIQNCSDKLAQNLSSKNPKHEHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVIHASLPKSMEGYYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTKETHVNNLYSMVHYCENITECRRIQLLAYFGEKGFNPDFCKKYPDVSCDNCCKTKDYKTKDVTDDVKNIIRFVQEHSSSPGTRNIGPAGRFTLNMLVDIFLGSKSAKVKSGIFGKGTTYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHLKVDFMETENSSSIKKQKALVAKVSQREEVVKKCLGELTEVCKLLGKVFGVHYFNIFNTATLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVIPVLQKYSEWTVPAEDGSPGARGAPEDTEEEEEEAPVSSHYFANQTRNERKRKKMSATHKPKRRRTSYGGFRAKGGSTTCRKTTSKSKFYGVTGSRSASCASQATSSASRKLGIMAPPKPVNRTFLRPSYAFS

Target ORF information:

RefSeq Version	NM_001042527.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bloom syndrome, RecQ like helicase (Blm), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042527.2

ORF Insert Sequence:

ATGGCTGCTG TTCCTCTGAA CAATCTACAA GAACAACTAC AGCGACACTC AGCCAGAAAA 
CTTAATAATC AACCCAGCCT TTCAAAACCA AAATCTTTAG GTTTTACTTT TAAAAAGAAA 
ACATCAGAGG GTGATGTGTC TGTCACTAGT GTGTCCGTAG TAAAAACACC TGCGTTAAGT 
GATAAAGATG TGAACGTGTC TGAGGCCTTT TCATTCACTG AGTCTCCACT CCACAAACCA 
AAGCAGCAGG CAAAGATTGA AGGCTTCTTT AAACATTTCC CTGGAAGGCA GCAAAGCAAG 
GGGACCTGCT CTGAGCCGTC ACTGCCGGCC ACGGTACAGA CTGCTCAGGA CACTTTGTGC 
ACTACCCCCA AAACCCCCAC TGCGAAGAAA CTGCCCGTGG CTGTTTTCAA GAAATTAGAA 
TTTAGTTCTT CTGCAGACTC CCTCAGTGAC TGGGCTGATA TGGATGACTT TGATATGTCA 
GCATCAGATG CGTTTGCTTC ACTGGCTAAA AATCCTGCCA CAAGAGTAAG CACCGCTCAG 
AAAATGAAAA AGACTAAGAG AAACTTCTTT AAACCACCAC CTCGTAAAGC CAATGCAGTA 
AAGACTGACT TGACTCCTCC CTCCCCCGAA TGCCTGCAAG TGGATTTAAC GAAGGAATCG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGCG GAGGGGGCGG ACTGCCTGAG CAGGGATGTG 
ATCTGCATTG ACAATGATTC TGCTTCTGAA GAGCTCACGG AGAAAGACAC GCAGGAAAGC 
CAGTCTTTGA AAGCTCACTT GGGAGCTGAA AGAGGTGACA GTGAAAAGAA GAGCCATGAA 
GACGAAGCTG TGTTCCATTC AGTTCAGAAC ACTGAATACT TTGAACACAA TGACAATGAT 
TATGATATAG ATTTTGTTCC ACCTTCTCCA GAAGAAATCA TCTCCACTGC TTCTTCCTCG 
TTGAAATGTT CCAGTATGTT AAAGGATCTT GATGACTCTG ACAAAGAAAA GGGCATTCTT 
AGCACCTCAG AAGAGCTTCT GTCAAAACCA GAGGAAATGA CCACACACAA GTCTGATGCA 
GGAACCAGTA AAGACTGTGA TGCCCAGCAG ATACGCATAC AGCAGCAGCT TATTCATGTG 
ATGGAGCACA TCTGTAAGTT AGTTGATACT GTTCCTACTG ATGAACTGGA AGCTTTGAAT 
TGTGGGACCG AATTGCTTCA ACAACGAAAC ATAAGGAGGA AGCTCCTAGC TGAAGCAGGT 
TTTAATGGAA ATGACGTCAG ACTTCTGGGT TCTCTGTGGA GGCACAGGCC TGATTCACTT 
GATAACACAG TGCAGGGCGA CTCCTGCCCT GTGGGGCATC CTAATAAAGA GTTAAATTCT 
CCATACCTTC TCTCACATTC CCCTTCCACT GAGGAATGTT TACCCACCAC CACTCCAGGA 
AAGACAGGAT TCTCAGCCAC CCCGAAGAAT CTCTTTGAAA GGCCGTTATT GAATTCCCAT 
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGGATGGA AAACAGGAAC 
GAAAGCACTG ACTTCCCAGG GAGTGTTCTC ACCAGCACCA CTGTGAAAGC TCAGAGTAAA 
CAAGCTGCTT CAGGATGGAA CGTAGAGAGA CACGGCCAGG CTTCCTATGA TATCGATAAC 
TTTAATATTG ATGACTTTGA TGATGATGAT GATGATGATG ACTGGGAAAA CATAATGCAC 
AATTTTCCAG CCAGCAAATC TTCCACAGCC ACCTACCCAC CCATCAAGGA AGGTGGGCCA 
GTTAAATCTC TCTCAGAAAG GATTTCTTCA GCCAAGGCAA AGTTTCTTCC AGTGGTATCA 
ACCGCTCAAA ATACAAACCT CTCAGAGTCA ATTCAGAATT GCTCTGATAA GCTGGCCCAA 
AATTTATCAT CAAAAAATCC AAAACATGAA CATTTTCAAA GTCTTAATTT TCCTCATACA 
AAAGAAATGA TGAAGATTTT CCATAAGAAA TTTGGCTTGC ATAATTTTAG AACTAATCAG 
CTAGAGGCGA TCAATGCTGC GCTGCTTGGT GAAGACTGCT TTATCCTAAT GCCCACTGGA 
GGAGGTAAAA GTTTGTGCTA CCAGCTCCCT GCCTGTGTTT CTCCTGGGGT CACAATTGTC 
ATTTCTCCCT TGAGATCACT AATAGTAGAT CAAGTCCAAA AGCTGACTTC CTTTGATATT 
CCAGCTACAT ATCTGACAGG GGATAAGACT GACTCAGAAG CTGCAAATAT TTACCTCCAA 
TTATCCAAAA AAGACCCAAT CATCAAGCTT TTGTATGTTA CTCCAGAGAA GGTCTGTGCA 
AGTAACAGGC TGATTTCTAC TCTGGAGAAT CTGTATGAGC GGAAGCTCTT GGCACGTTTT 
GTCATTGATG AAGCGCATTG TGTGAGTCAG TGGGGTCATG ATTTTCGTCA AGATTACAAA 
AGGATGAATA TGCTTCGCCA GAAGTTTCCT TCTGTTCCAG TGATGGCCCT CACGGCCACA 
GCGAACCCCA GGGTCCAGAA GGACATCCTC ACTCAGCTGA AGATCCTCAG ACCTCAGGTG 
TTTAGCATGA GCTTTAACAG ACACAATCTG AAGTACTATG TATTACCCAA GAAGCCCAAA 
AAAGTAGCAT TTGATTGCCT AGAGTGGATC AGAAAGCATC ACCCTTATGA CTCGGGGATA 
ATTTACTGCC TCTCCAGGAG GGAATGTGAC ACAATGGCTG ACACTTTACA GAGAGAAGGC 
CTGGCTGCCC TGGCTTACCA TGCGGGCCTC AGTGACTCTG CCAGAGATGA GGTGCAGCAC 
AAGTGGATCA ACCAGGACAA CTGCCAGGTT ATCTGTGCGA CAATTGCGTT TGGAATGGGA 
ATTGACAAAC CTGACGTGCG ATTTGTGATT CATGCATCTC TTCCTAAATC TATGGAGGGT 
TATTATCAAG AATCCGGCCG AGCTGGAAGA GATGGGGAAA TATCTCACTG CGTGCTTTTC 
TATACATATC ATGATGTGAC CAGACTGAAG AGACTTATAA TGATGGAAAA AGATGGAAAC 
TATCATACGA AGGAAACTCA CGTCAATAAT CTATATAGCA TGGTACATTA CTGTGAAAAC 
ATAACGGAAT GCAGAAGAAT ACAGCTTTTA GCTTACTTTG GTGAAAAAGG ATTCAACCCT 
GATTTTTGTA AGAAATACCC AGATGTTTCT TGTGACAATT GCTGTAAAAC AAAGGATTAT 
AAAACAAAAG ATGTGACTGA TGACGTGAAA AATATTATAA GATTTGTTCA AGAACACAGT 
TCATCACCAG GAACAAGAAA TATAGGACCT GCTGGAAGAT TTACTCTGAA CATGCTGGTC 
GACATTTTCT TGGGGAGCAA GAGTGCAAAA GTTAAGTCTG GAATATTTGG AAAGGGGACT 
ACATATTCAC GACATAATGC CGAAAGACTT TTTAAAAAGC TGATTCTAGA CAAAATCCTG 
GATGAAGACT TATATATCAA TGCCAATGAC CAACCAATTG CCTATGTGAT GCTAGGAACA 
AAAGCCCACA GTGTGCTGAG TGGCCACTTG AAGGTGGACT TCATGGAAAC GGAAAATTCC 
AGCAGTATTA AAAAACAAAA AGCTTTAGTG GCCAAAGTAT CCCAGAGAGA AGAGGTAGTT 
AAGAAATGTC TTGGAGAACT TACAGAGGTC TGCAAATTGC TGGGGAAAGT CTTTGGTGTC 
CATTACTTCA ATATTTTTAA TACAGCCACA CTCAAAAAGC TTGCAGAATC TTTATCTTCT 
GATCCTGAGG TTTTGCTTCA GATTGATGGT GTTACCGAAG ACAAGCTGGA AAAATATGGT 
GCAGAAGTGA TTCCAGTATT ACAGAAGTAC TCAGAATGGA CAGTGCCAGC TGAGGATGGT 
TCCCCAGGCG CCAGAGGCGC CCCAGAGGAC ACTGAGGAGG AGGAGGAGGA AGCGCCTGTA 
TCTTCTCACT ACTTTGCAAA TCAAACTAGA AATGAAAGAA AGAGAAAGAA AATGTCAGCC 
ACCCATAAGC CCAAGAGGAG AAGAACTAGT TACGGTGGCT TCAGAGCAAA GGGGGGCTCT 
ACTACATGCA GAAAAACGAC TTCTAAAAGT AAATTCTATG GTGTAACTGG ATCCCGCTCG 
GCCTCATGTG CTTCTCAGGC AACATCATCA GCCAGTAGAA AACTGGGGAT TATGGCTCCT 
CCAAAGCCTG TAAATAGAAC GTTCCTCAGG CCTTCATATG CCTTCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blm ( NM_007550.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031576.4 Mus musculus Bloom syndrome, RecQ like helicase (Blm), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16148
Clone ID Related Accession (Same CDS sequence)	NM_007550.4
Accession Version	NM_007550.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Mus musculus(house mouse)
Product	Bloom syndrome protein homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB614580.1, Z98263.1 and AC110907.7. On Apr 14, 2009 this sequence version replaced NM_007550.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB008674.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGATCA TGGCTGCTGT TCCTCTGAAC AATCTACAAG AACAACTACA GCGACACTCA 
GCCAGAAAAC TTAATAATCA ACCCAGCCTT TCAAAACCAA AATCTTTAGG TTTTACTTTT 
AAAAAGAAAA CATCAGAGGG TGATGTGTCT GTCACTAGTG TGTCCGTAGT AAAAACACCT 
GCGTTAAGTG ATAAAGATGT GAACGTGTCT GAGGCCTTTT CATTCACTGA GTCTCCACTC 
CACAAACCAA AGCAGCAGGC AAAGATTGAA GGCTTCTTTA AACATTTCCC TGGAAGGCAG 
CAAAGCAAGG GGACCTGCTC TGAGCCGTCA CTGCCGGCCA CGGTACAGAC TGCTCAGGAC 
ACTTTGTGCA CTACCCCCAA AACCCCCACT GCGAAGAAAC TGCCCGTGGC TGTTTTCAAG 
AAATTAGAAT TTAGTTCTTC TGCAGACTCC CTCAGTGACT GGGCTGATAT GGATGACTTT 
GATATGTCAG CATCAGATGC GTTTGCTTCA CTGGCTAAAA ATCCTGCCAC AAGAGTAAGC 
ACCGCTCAGA AAATGAAAAA GACTAAGAGA AACTTCTTTA AACCACCACC TCGTAAAGCC 
AATGCAGTAA AGACTGACTT GACTCCTCCC TCCCCCGAAT GCCTGCAAGT GGATTTAACG 
AAGGAATCGG AGGAGGAGGA GGAGGAGGAG GAGGAGGCGG AGGGGGCGGA CTGCCTGAGC 
AGGGATGTGA TCTGCATTGA CAATGATTCT GCTTCTGAAG AGCTCACGGA GAAAGACACG 
CAGGAAAGCC AGTCTTTGAA AGCTCACTTG GGAGCTGAAA GAGGTGACAG TGAAAAGAAG 
AGCCATGAAG ACGAAGCTGT GTTCCATTCA GTTCAGAACA CTGAATACTT TGAACACAAT 
GACAATGATT ATGATATAGA TTTTGTTCCA CCTTCTCCAG AAGAAATCAT CTCCACTGCT 
TCTTCCTCGT TGAAATGTTC CAGTATGTTA AAGGATCTTG ATGACTCTGA CAAAGAAAAG 
GGCATTCTTA GCACCTCAGA AGAGCTTCTG TCAAAACCAG AGGAAATGAC CACACACAAG 
TCTGATGCAG GAACCAGTAA AGACTGTGAT GCCCAGCAGA TACGCATACA GCAGCAGCTT 
ATTCATGTGA TGGAGCACAT CTGTAAGTTA GTTGATACTG TTCCTACTGA TGAACTGGAA 
GCTTTGAATT GTGGGACCGA ATTGCTTCAA CAACGAAACA TAAGGAGGAA GCTCCTAGCT 
GAAGCAGGTT TTAATGGAAA TGACGTCAGA CTTCTGGGTT CTCTGTGGAG GCACAGGCCT 
GATTCACTTG ATAACACAGT GCAGGGCGAC TCCTGCCCTG TGGGGCATCC TAATAAAGAG 
TTAAATTCTC CATACCTTCT CTCACATTCC CCTTCCACTG AGGAATGTTT ACCCACCACC 
ACTCCAGGAA AGACAGGATT CTCAGCCACC CCGAAGAATC TCTTTGAAAG GCCGTTATTG 
AATTCCCATT TACAGAAGTC CTTTGTAAGT AGCAACTGGG CTGAAACACC AAGGATGGAA 
AACAGGAACG AAAGCACTGA CTTCCCAGGG AGTGTTCTCA CCAGCACCAC TGTGAAAGCT 
CAGAGTAAAC AAGCTGCTTC AGGATGGAAC GTAGAGAGAC ACGGCCAGGC TTCCTATGAT 
ATCGATAACT TTAATATTGA TGACTTTGAT GATGATGATG ATGATGATGA CTGGGAAAAC 
ATAATGCACA ATTTTCCAGC CAGCAAATCT TCCACAGCCA CCTACCCACC CATCAAGGAA 
GGTGGGCCAG TTAAATCTCT CTCAGAAAGG ATTTCTTCAG CCAAGGCAAA GTTTCTTCCA 
GTGGTATCAA CCGCTCAAAA TACAAACCTC TCAGAGTCAA TTCAGAATTG CTCTGATAAG 
CTGGCCCAAA ATTTATCATC AAAAAATCCA AAACATGAAC ATTTTCAAAG TCTTAATTTT 
CCTCATACAA AAGAAATGAT GAAGATTTTC CATAAGAAAT TTGGCTTGCA TAATTTTAGA 
ACTAATCAGC TAGAGGCGAT CAATGCTGCG CTGCTTGGTG AAGACTGCTT TATCCTAATG 
CCCACTGGAG GAGGTAAAAG TTTGTGCTAC CAGCTCCCTG CCTGTGTTTC TCCTGGGGTC 
ACAATTGTCA TTTCTCCCTT GAGATCACTA ATAGTAGATC AAGTCCAAAA GCTGACTTCC 
TTTGATATTC CAGCTACATA TCTGACAGGG GATAAGACTG ACTCAGAAGC TGCAAATATT 
TACCTCCAAT TATCCAAAAA AGACCCAATC ATCAAGCTTT TGTATGTTAC TCCAGAGAAG 
GTCTGTGCAA GTAACAGGCT GATTTCTACT CTGGAGAATC TGTATGAGCG GAAGCTCTTG 
GCACGTTTTG TCATTGATGA AGCGCATTGT GTGAGTCAGT GGGGTCATGA TTTTCGTCAA 
GATTACAAAA GGATGAATAT GCTTCGCCAG AAGTTTCCTT CTGTTCCAGT GATGGCCCTC 
ACGGCCACAG CGAACCCCAG GGTCCAGAAG GACATCCTCA CTCAGCTGAA GATCCTCAGA 
CCTCAGGTGT TTAGCATGAG CTTTAACAGA CACAATCTGA AGTACTATGT ATTACCCAAG 
AAGCCCAAAA AAGTAGCATT TGATTGCCTA GAGTGGATCA GAAAGCATCA CCCTTATGAC 
TCGGGGATAA TTTACTGCCT CTCCAGGAGG GAATGTGACA CAATGGCTGA CACTTTACAG 
AGAGAAGGCC TGGCTGCCCT GGCTTACCAT GCGGGCCTCA GTGACTCTGC CAGAGATGAG 
GTGCAGCACA AGTGGATCAA CCAGGACAAC TGCCAGGTTA TCTGTGCGAC AATTGCGTTT 
GGAATGGGAA TTGACAAACC TGACGTGCGA TTTGTGATTC ATGCATCTCT TCCTAAATCT 
ATGGAGGGTT ATTATCAAGA ATCCGGCCGA GCTGGAAGAG ATGGGGAAAT ATCTCACTGC 
GTGCTTTTCT ATACATATCA TGATGTGACC AGACTGAAGA GACTTATAAT GATGGAAAAA 
GATGGAAACT ATCATACGAA GGAAACTCAC GTCAATAATC TATATAGCAT GGTACATTAC 
TGTGAAAACA TAACGGAATG CAGAAGAATA CAGCTTTTAG CTTACTTTGG TGAAAAAGGA 
TTCAACCCTG ATTTTTGTAA GAAATACCCA GATGTTTCTT GTGACAATTG CTGTAAAACA 
AAGGATTATA AAACAAAAGA TGTGACTGAT GACGTGAAAA ATATTATAAG ATTTGTTCAA 
GAACACAGTT CATCACCAGG AACAAGAAAT ATAGGACCTG CTGGAAGATT TACTCTGAAC 
ATGCTGGTCG ACATTTTCTT GGGGAGCAAG AGTGCAAAAG TTAAGTCTGG AATATTTGGA 
AAGGGGACTA CATATTCACG ACATAATGCC GAAAGACTTT TTAAAAAGCT GATTCTAGAC 
AAAATCCTGG ATGAAGACTT ATATATCAAT GCCAATGACC AACCAATTGC CTATGTGATG 
CTAGGAACAA AAGCCCACAG TGTGCTGAGT GGCCACTTGA AGGTGGACTT CATGGAAACG 
GAAAATTCCA GCAGTATTAA AAAACAAAAA GCTTTAGTGG CCAAAGTATC CCAGAGAGAA 
GAGGTAGTTA AGAAATGTCT TGGAGAACTT ACAGAGGTCT GCAAATTGCT GGGGAAAGTC 
TTTGGTGTCC ATTACTTCAA TATTTTTAAT ACAGCCACAC TCAAAAAGCT TGCAGAATCT 
TTATCTTCTG ATCCTGAGGT TTTGCTTCAG ATTGATGGTG TTACCGAAGA CAAGCTGGAA 
AAATATGGTG CAGAAGTGAT TCCAGTATTA CAGAAGTACT CAGAATGGAC AGTGCCAGCT 
GAGGATGGTT CCCCAGGCGC CAGAGGCGCC CCAGAGGACA CTGAGGAGGA GGAGGAGGAA 
GCGCCTGTAT CTTCTCACTA CTTTGCAAAT CAAACTAGAA ATGAAAGAAA GAGAAAGAAA 
ATGTCAGCCA CCCATAAGCC CAAGAGGAGA AGAACTAGTT ACGGTGGCTT CAGAGCAAAG 
GGGGGCTCTA CTACATGCAG AAAAACGACT TCTAAAAGTA AATTCTATGG TGTAACTGGA 
TCCCGCTCGG CCTCATGTGC TTCTCAGGCA ACATCATCAG CCAGTAGAAA ACTGGGGATT 
ATGGCTCCTC CAAAGCCTGT AAATAGAACG TTCCTCAGGC CTTCATATGC CTTCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031576.4
CDS	344..4603
Translation	MRIMAAVPLNNLQEQLQRHSARKLNNQPSLSKPKSLGFTFKKKTSEGDVSVTSVSVVKTPALSDKDVNVSEAFSFTESPLHKPKQQAKIEGFFKHFPGRQQSKGTCSEPSLPATVQTAQDTLCTTPKTPTAKKLPVAVFKKLEFSSSADSLSDWADMDDFDMSASDAFASLAKNPATRVSTAQKMKKTKRNFFKPPPRKANAVKTDLTPPSPECLQVDLTKESEEEEEEEEEAEGADCLSRDVICIDNDSASEELTEKDTQESQSLKAHLGAERGDSEKKSHEDEAVFHSVQNTEYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKGILSTSEELLSKPEEMTTHKSDAGTSKDCDAQQIRIQQQLIHVMEHICKLVDTVPTDELEALNCGTELLQQRNIRRKLLAEAGFNGNDVRLLGSLWRHRPDSLDNTVQGDSCPVGHPNKELNSPYLLSHSPSTEECLPTTTPGKTGFSATPKNLFERPLLNSHLQKSFVSSNWAETPRMENRNESTDFPGSVLTSTTVKAQSKQAASGWNVERHGQASYDIDNFNIDDFDDDDDDDDWENIMHNFPASKSSTATYPPIKEGGPVKSLSERISSAKAKFLPVVSTAQNTNLSESIQNCSDKLAQNLSSKNPKHEHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVIHASLPKSMEGYYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTKETHVNNLYSMVHYCENITECRRIQLLAYFGEKGFNPDFCKKYPDVSCDNCCKTKDYKTKDVTDDVKNIIRFVQEHSSSPGTRNIGPAGRFTLNMLVDIFLGSKSAKVKSGIFGKGTTYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHLKVDFMETENSSSIKKQKALVAKVSQREEVVKKCLGELTEVCKLLGKVFGVHYFNIFNTATLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVIPVLQKYSEWTVPAEDGSPGARGAPEDTEEEEEEAPVSSHYFANQTRNERKRKKMSATHKPKRRRTSYGGFRAKGGSTTCRKTTSKSKFYGVTGSRSASCASQATSSASRKLGIMAPPKPVNRTFLRPSYAFS

Target ORF information:

RefSeq Version	NM_007550.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bloom syndrome, RecQ like helicase (Blm), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007550.4

ORF Insert Sequence:

ATGAGGATCA TGGCTGCTGT TCCTCTGAAC AATCTACAAG AACAACTACA GCGACACTCA 
GCCAGAAAAC TTAATAATCA ACCCAGCCTT TCAAAACCAA AATCTTTAGG TTTTACTTTT 
AAAAAGAAAA CATCAGAGGG TGATGTGTCT GTCACTAGTG TGTCCGTAGT AAAAACACCT 
GCGTTAAGTG ATAAAGATGT GAACGTGTCT GAGGCCTTTT CATTCACTGA GTCTCCACTC 
CACAAACCAA AGCAGCAGGC AAAGATTGAA GGCTTCTTTA AACATTTCCC TGGAAGGCAG 
CAAAGCAAGG GGACCTGCTC TGAGCCGTCA CTGCCGGCCA CGGTACAGAC TGCTCAGGAC 
ACTTTGTGCA CTACCCCCAA AACCCCCACT GCGAAGAAAC TGCCCGTGGC TGTTTTCAAG 
AAATTAGAAT TTAGTTCTTC TGCAGACTCC CTCAGTGACT GGGCTGATAT GGATGACTTT 
GATATGTCAG CATCAGATGC GTTTGCTTCA CTGGCTAAAA ATCCTGCCAC AAGAGTAAGC 
ACCGCTCAGA AAATGAAAAA GACTAAGAGA AACTTCTTTA AACCACCACC TCGTAAAGCC 
AATGCAGTAA AGACTGACTT GACTCCTCCC TCCCCCGAAT GCCTGCAAGT GGATTTAACG 
AAGGAATCGG AGGAGGAGGA GGAGGAGGAG GAGGAGGCGG AGGGGGCGGA CTGCCTGAGC 
AGGGATGTGA TCTGCATTGA CAATGATTCT GCTTCTGAAG AGCTCACGGA GAAAGACACG 
CAGGAAAGCC AGTCTTTGAA AGCTCACTTG GGAGCTGAAA GAGGTGACAG TGAAAAGAAG 
AGCCATGAAG ACGAAGCTGT GTTCCATTCA GTTCAGAACA CTGAATACTT TGAACACAAT 
GACAATGATT ATGATATAGA TTTTGTTCCA CCTTCTCCAG AAGAAATCAT CTCCACTGCT 
TCTTCCTCGT TGAAATGTTC CAGTATGTTA AAGGATCTTG ATGACTCTGA CAAAGAAAAG 
GGCATTCTTA GCACCTCAGA AGAGCTTCTG TCAAAACCAG AGGAAATGAC CACACACAAG 
TCTGATGCAG GAACCAGTAA AGACTGTGAT GCCCAGCAGA TACGCATACA GCAGCAGCTT 
ATTCATGTGA TGGAGCACAT CTGTAAGTTA GTTGATACTG TTCCTACTGA TGAACTGGAA 
GCTTTGAATT GTGGGACCGA ATTGCTTCAA CAACGAAACA TAAGGAGGAA GCTCCTAGCT 
GAAGCAGGTT TTAATGGAAA TGACGTCAGA CTTCTGGGTT CTCTGTGGAG GCACAGGCCT 
GATTCACTTG ATAACACAGT GCAGGGCGAC TCCTGCCCTG TGGGGCATCC TAATAAAGAG 
TTAAATTCTC CATACCTTCT CTCACATTCC CCTTCCACTG AGGAATGTTT ACCCACCACC 
ACTCCAGGAA AGACAGGATT CTCAGCCACC CCGAAGAATC TCTTTGAAAG GCCGTTATTG 
AATTCCCATT TACAGAAGTC CTTTGTAAGT AGCAACTGGG CTGAAACACC AAGGATGGAA 
AACAGGAACG AAAGCACTGA CTTCCCAGGG AGTGTTCTCA CCAGCACCAC TGTGAAAGCT 
CAGAGTAAAC AAGCTGCTTC AGGATGGAAC GTAGAGAGAC ACGGCCAGGC TTCCTATGAT 
ATCGATAACT TTAATATTGA TGACTTTGAT GATGATGATG ATGATGATGA CTGGGAAAAC 
ATAATGCACA ATTTTCCAGC CAGCAAATCT TCCACAGCCA CCTACCCACC CATCAAGGAA 
GGTGGGCCAG TTAAATCTCT CTCAGAAAGG ATTTCTTCAG CCAAGGCAAA GTTTCTTCCA 
GTGGTATCAA CCGCTCAAAA TACAAACCTC TCAGAGTCAA TTCAGAATTG CTCTGATAAG 
CTGGCCCAAA ATTTATCATC AAAAAATCCA AAACATGAAC ATTTTCAAAG TCTTAATTTT 
CCTCATACAA AAGAAATGAT GAAGATTTTC CATAAGAAAT TTGGCTTGCA TAATTTTAGA 
ACTAATCAGC TAGAGGCGAT CAATGCTGCG CTGCTTGGTG AAGACTGCTT TATCCTAATG 
CCCACTGGAG GAGGTAAAAG TTTGTGCTAC CAGCTCCCTG CCTGTGTTTC TCCTGGGGTC 
ACAATTGTCA TTTCTCCCTT GAGATCACTA ATAGTAGATC AAGTCCAAAA GCTGACTTCC 
TTTGATATTC CAGCTACATA TCTGACAGGG GATAAGACTG ACTCAGAAGC TGCAAATATT 
TACCTCCAAT TATCCAAAAA AGACCCAATC ATCAAGCTTT TGTATGTTAC TCCAGAGAAG 
GTCTGTGCAA GTAACAGGCT GATTTCTACT CTGGAGAATC TGTATGAGCG GAAGCTCTTG 
GCACGTTTTG TCATTGATGA AGCGCATTGT GTGAGTCAGT GGGGTCATGA TTTTCGTCAA 
GATTACAAAA GGATGAATAT GCTTCGCCAG AAGTTTCCTT CTGTTCCAGT GATGGCCCTC 
ACGGCCACAG CGAACCCCAG GGTCCAGAAG GACATCCTCA CTCAGCTGAA GATCCTCAGA 
CCTCAGGTGT TTAGCATGAG CTTTAACAGA CACAATCTGA AGTACTATGT ATTACCCAAG 
AAGCCCAAAA AAGTAGCATT TGATTGCCTA GAGTGGATCA GAAAGCATCA CCCTTATGAC 
TCGGGGATAA TTTACTGCCT CTCCAGGAGG GAATGTGACA CAATGGCTGA CACTTTACAG 
AGAGAAGGCC TGGCTGCCCT GGCTTACCAT GCGGGCCTCA GTGACTCTGC CAGAGATGAG 
GTGCAGCACA AGTGGATCAA CCAGGACAAC TGCCAGGTTA TCTGTGCGAC AATTGCGTTT 
GGAATGGGAA TTGACAAACC TGACGTGCGA TTTGTGATTC ATGCATCTCT TCCTAAATCT 
ATGGAGGGTT ATTATCAAGA ATCCGGCCGA GCTGGAAGAG ATGGGGAAAT ATCTCACTGC 
GTGCTTTTCT ATACATATCA TGATGTGACC AGACTGAAGA GACTTATAAT GATGGAAAAA 
GATGGAAACT ATCATACGAA GGAAACTCAC GTCAATAATC TATATAGCAT GGTACATTAC 
TGTGAAAACA TAACGGAATG CAGAAGAATA CAGCTTTTAG CTTACTTTGG TGAAAAAGGA 
TTCAACCCTG ATTTTTGTAA GAAATACCCA GATGTTTCTT GTGACAATTG CTGTAAAACA 
AAGGATTATA AAACAAAAGA TGTGACTGAT GACGTGAAAA ATATTATAAG ATTTGTTCAA 
GAACACAGTT CATCACCAGG AACAAGAAAT ATAGGACCTG CTGGAAGATT TACTCTGAAC 
ATGCTGGTCG ACATTTTCTT GGGGAGCAAG AGTGCAAAAG TTAAGTCTGG AATATTTGGA 
AAGGGGACTA CATATTCACG ACATAATGCC GAAAGACTTT TTAAAAAGCT GATTCTAGAC 
AAAATCCTGG ATGAAGACTT ATATATCAAT GCCAATGACC AACCAATTGC CTATGTGATG 
CTAGGAACAA AAGCCCACAG TGTGCTGAGT GGCCACTTGA AGGTGGACTT CATGGAAACG 
GAAAATTCCA GCAGTATTAA AAAACAAAAA GCTTTAGTGG CCAAAGTATC CCAGAGAGAA 
GAGGTAGTTA AGAAATGTCT TGGAGAACTT ACAGAGGTCT GCAAATTGCT GGGGAAAGTC 
TTTGGTGTCC ATTACTTCAA TATTTTTAAT ACAGCCACAC TCAAAAAGCT TGCAGAATCT 
TTATCTTCTG ATCCTGAGGT TTTGCTTCAG ATTGATGGTG TTACCGAAGA CAAGCTGGAA 
AAATATGGTG CAGAAGTGAT TCCAGTATTA CAGAAGTACT CAGAATGGAC AGTGCCAGCT 
GAGGATGGTT CCCCAGGCGC CAGAGGCGCC CCAGAGGACA CTGAGGAGGA GGAGGAGGAA 
GCGCCTGTAT CTTCTCACTA CTTTGCAAAT CAAACTAGAA ATGAAAGAAA GAGAAAGAAA 
ATGTCAGCCA CCCATAAGCC CAAGAGGAGA AGAACTAGTT ACGGTGGCTT CAGAGCAAAG 
GGGGGCTCTA CTACATGCAG AAAAACGACT TCTAAAAGTA AATTCTATGG TGTAACTGGA 
TCCCGCTCGG CCTCATGTGC TTCTCAGGCA ACATCATCAG CCAGTAGAAA ACTGGGGATT 
ATGGCTCCTC CAAAGCCTGT AAATAGAACG TTCCTCAGGC CTTCATATGC CTTCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BLM helicase suppresses recombination at G-quadruplex motifs in transcribed genes.
Nature communications9(1)271(2018 Jan)
van Wietmarschen N,Merzouk S,Halsema N,Spierings DCJ,Guryev V,Lansdorp PM

BLM helicase regulates DNA repair by counteracting RAD51 loading at DNA double-strand break sites.
The Journal of cell biology216(11)3521-3534(2017 Nov)
Patel DS,Misenko SM,Her J,Bunting SF

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Blm cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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