BLM gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011521882.2	XP_011520184.1	Bloom syndrome protein isoform X1
NM_001287246.1	NP_001274175.1	Bloom syndrome protein isoform 1
XM_011521882.3	XP_011520184.1	Bloom syndrome protein isoform X1
XM_006720632.2	XP_006720695.1	Bloom syndrome protein isoform X3
NM_001287246.2	NP_001274175.1	Bloom syndrome protein isoform 1
NM_001287248.2	NP_001274177.1	Bloom syndrome protein isoform 3
NM_001287247.1	NP_001274176.1	Bloom syndrome protein isoform 2
XM_011521881.2	XP_011520183.1	Bloom syndrome protein isoform X2
NM_000057.4	NP_000048.1	Bloom syndrome protein isoform 1
NM_001287247.2	NP_001274176.1	Bloom syndrome protein isoform 2
NM_000057.3	NP_000048.1	Bloom syndrome protein isoform 1
NM_001287248.1	NP_001274177.1	Bloom syndrome protein isoform 3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu48483	XM_006720632.2 Latest version!	Homo sapiens BLM RecQ like helicase (BLM), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu56490	XM_011521881.2 Latest version!	Homo sapiens BLM RecQ like helicase (BLM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OHu56491	XM_011521882.3 Latest version!	Homo sapiens BLM RecQ like helicase (BLM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu20445	NM_000057.4 Latest version!	Homo sapiens BLM RecQ like helicase (BLM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OHu20445	NM_001287246.2 Latest version!	Homo sapiens BLM RecQ like helicase (BLM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OHu20947	NM_001287248.2 Latest version!	Homo sapiens BLM RecQ like helicase (BLM), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu20391	NM_001287247.2 Latest version!	Homo sapiens BLM RecQ like helicase (BLM), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu56491	XM_011521882.2	Homo sapiens Bloom syndrome RecQ like helicase (BLM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu20445	NM_001287246.1	Homo sapiens BLM RecQ like helicase (BLM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OHu20391	NM_001287247.1	Homo sapiens BLM RecQ like helicase (BLM), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu20445	NM_000057.3	Homo sapiens Bloom syndrome RecQ like helicase (BLM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OHu20947	NM_001287248.1	Homo sapiens BLM RecQ like helicase (BLM), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
Hide Old Accession Versions >>

BLM ( XM_006720632.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720695.1 Homo sapiens BLM RecQ like helicase (BLM), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu48483
Clone ID Related Accession (Same CDS sequence)	XM_006720632.2
Accession Version	XM_006720632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	Bloom syndrome protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006720632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA TTTTTCATAA AAAATTTGGC CTGCATAATT TTAGAACTAA TCAGCTAGAG 
GCGATCAATG CTGCACTGCT TGGTGAAGAC TGTTTTATCC TGATGCCGAC TGGAGGTGGT 
AAGAGTTTGT GTTACCAGCT CCCTGCCTGT GTTTCTCCTG GGGTCACTGT TGTCATTTCT 
CCCTTGAGAT CACTTATCGT AGATCAAGTC CAAAAGCTGA CTTCCTTGGA TATTCCAGCT 
ACATATCTGA CAGGTGATAA GACTGACTCA GAAGCTACAA ATATTTACCT CCAGTTATCA 
AAAAAAGACC CAATCATAAA ACTTCTATAT GTCACTCCAG AAAAGATCTG TGCAAGTAAC 
AGACTCATTT CTACTCTGGA GAATCTCTAT GAGAGGAAGC TCTTGGCACG TTTTGTTATT 
GATGAAGCAC ATTGTGTCAG TCAGTGGGGA CATGATTTTC GTCAAGATTA CAAAAGAATG 
AATATGCTTC GCCAGAAGTT TCCTTCTGTT CCGGTGATGG CTCTTACGGC CACAGCTAAT 
CCCAGGGTAC AGAAGGACAT CCTGACTCAG CTGAAGATTC TCAGACCTCA GGTGTTTAGC 
ATGAGCTTTA ACAGACATAA TCTGAAATAC TATGTATTAC CGAAAAAGCC TAAAAAGGTG 
GCATTTGATT GCCTAGAATG GATCAGAAAG CACCACCCAT ATGATTCAGG GATAATTTAC 
TGCCTCTCCA GGCGAGAATG TGACACCATG GCTGACACGT TACAGAGAGA TGGGCTCGCT 
GCTCTTGCTT ACCATGCTGG CCTCAGTGAT TCTGCCAGAG ATGAAGTGCA GCAGAAGTGG 
ATTAATCAGG ATGGCTGTCA GGTTATCTGT GCTACAATTG CATTTGGAAT GGGGATTGAC 
AAACCGGACG TGCGATTTGT GATTCATGCA TCTCTCCCTA AATCTGTGGA GGGTTACTAC 
CAAGAATCTG GCAGAGCTGG AAGAGATGGG GAAATATCTC ACTGCCTGCT TTTCTATACC 
TATCATGATG TGACCAGACT GAAAAGACTT ATAATGATGG AAAAAGATGG AAACCATCAT 
ACAAGAGAAA CTCACTTCAA TAATTTGTAT AGCATGGTAC ATTACTGTGA AAATATAACG 
GAATGCAGGA GAATACAGCT TTTGGCCTAC TTTGGTGAAA ATGGATTTAA TCCTGATTTT 
TGTAAGAAAC ACCCAGATGT TTCTTGTGAT AATTGCTGTA AAACAAAGGA TTATAAAACA 
AGAGATGTGA CTGACGATGT GAAAAGTATT GTAAGATTTG TTCAAGAACA TAGTTCATCA 
CAAGGAATGA GAAATATAAA ACATGTAGGT CCTTCTGGAA GATTTACTAT GAATATGCTG 
GTCGACATTT TCTTGGGGAG TAAGAGTGCA AAAATCCAGT CAGGTATATT TGGAAAAGGA 
TCTGCTTATT CACGACACAA TGCCGAAAGA CTTTTTAAAA AGCTGATACT TGACAAGATT 
TTGGATGAAG ACTTATATAT CAATGCCAAT GACCAGGCGA TCGCTTATGT GATGCTCGGA 
AATAAAGCCC AAACTGTACT AAATGGCAAT TTAAAGGTAG ACTTTATGGA AACAGAAAAT 
TCCAGCAGTG TGAAAAAACA AAAAGCGTTA GTAGCAAAAG TGTCTCAGAG GGAAGAGATG 
GTTAAAAAAT GTCTTGGAGA ACTTACAGAA GTCTGCAAAT CTCTGGGGAA AGTTTTTGGT 
GTCCATTACT TCAATATTTT TAATACCGTC ACTCTCAAGA AGCTTGCAGA ATCTTTATCT 
TCTGATCCTG AGGTTTTGCT TCAAATTGAT GGTGTTACTG AAGACAAACT GGAAAAATAT 
GGTGCGGAAG TGATTTCAGT ATTACAGAAA TACTCTGAAT GGACATCGCC AGCTGAAGAC 
AGTTCCCCAG GGATAAGCCT GTCCAGCAGC AGAGGCCCCG GAAGAAGTGC CGCTGAGGAG 
CTCGACGAGG AAATACCCGT ATCTTCCCAC TACTTTGCAA GTAAAACCAG AAATGAAAGG 
AAGAGGAAAA AGATGCCAGC CTCCCAAAGG TCTAAGAGGA GAAAAACTGC TTCCAGTGGT 
TCCAAGGCAA AGGGGGGGTC TGCCACATGT AGAAAGATAT CTTCCAAAAC GAAATCCTCC 
AGCATCATTG GATCCAGTTC AGCCTCACAT ACTTCTCAAG CGACATCAGG AGCCAATAGC 
AAATTGGGGA TTATGGCTCC ACCGAAGCCT ATAAATAGAC CGTTTCTTAA GCCTTCATAT 
GCATTCTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720695.1
CDS	140..2431
Translation	MMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	XM_006720632.2
Organism	Homo sapiens(human)
Definition	Homo sapiens BLM RecQ like helicase (BLM), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720632.2

ORF Insert Sequence:

ATGATGAAGA TTTTTCATAA AAAATTTGGC CTGCATAATT TTAGAACTAA TCAGCTAGAG 
GCGATCAATG CTGCACTGCT TGGTGAAGAC TGTTTTATCC TGATGCCGAC TGGAGGTGGT 
AAGAGTTTGT GTTACCAGCT CCCTGCCTGT GTTTCTCCTG GGGTCACTGT TGTCATTTCT 
CCCTTGAGAT CACTTATCGT AGATCAAGTC CAAAAGCTGA CTTCCTTGGA TATTCCAGCT 
ACATATCTGA CAGGTGATAA GACTGACTCA GAAGCTACAA ATATTTACCT CCAGTTATCA 
AAAAAAGACC CAATCATAAA ACTTCTATAT GTCACTCCAG AAAAGATCTG TGCAAGTAAC 
AGACTCATTT CTACTCTGGA GAATCTCTAT GAGAGGAAGC TCTTGGCACG TTTTGTTATT 
GATGAAGCAC ATTGTGTCAG TCAGTGGGGA CATGATTTTC GTCAAGATTA CAAAAGAATG 
AATATGCTTC GCCAGAAGTT TCCTTCTGTT CCGGTGATGG CTCTTACGGC CACAGCTAAT 
CCCAGGGTAC AGAAGGACAT CCTGACTCAG CTGAAGATTC TCAGACCTCA GGTGTTTAGC 
ATGAGCTTTA ACAGACATAA TCTGAAATAC TATGTATTAC CGAAAAAGCC TAAAAAGGTG 
GCATTTGATT GCCTAGAATG GATCAGAAAG CACCACCCAT ATGATTCAGG GATAATTTAC 
TGCCTCTCCA GGCGAGAATG TGACACCATG GCTGACACGT TACAGAGAGA TGGGCTCGCT 
GCTCTTGCTT ACCATGCTGG CCTCAGTGAT TCTGCCAGAG ATGAAGTGCA GCAGAAGTGG 
ATTAATCAGG ATGGCTGTCA GGTTATCTGT GCTACAATTG CATTTGGAAT GGGGATTGAC 
AAACCGGACG TGCGATTTGT GATTCATGCA TCTCTCCCTA AATCTGTGGA GGGTTACTAC 
CAAGAATCTG GCAGAGCTGG AAGAGATGGG GAAATATCTC ACTGCCTGCT TTTCTATACC 
TATCATGATG TGACCAGACT GAAAAGACTT ATAATGATGG AAAAAGATGG AAACCATCAT 
ACAAGAGAAA CTCACTTCAA TAATTTGTAT AGCATGGTAC ATTACTGTGA AAATATAACG 
GAATGCAGGA GAATACAGCT TTTGGCCTAC TTTGGTGAAA ATGGATTTAA TCCTGATTTT 
TGTAAGAAAC ACCCAGATGT TTCTTGTGAT AATTGCTGTA AAACAAAGGA TTATAAAACA 
AGAGATGTGA CTGACGATGT GAAAAGTATT GTAAGATTTG TTCAAGAACA TAGTTCATCA 
CAAGGAATGA GAAATATAAA ACATGTAGGT CCTTCTGGAA GATTTACTAT GAATATGCTG 
GTCGACATTT TCTTGGGGAG TAAGAGTGCA AAAATCCAGT CAGGTATATT TGGAAAAGGA 
TCTGCTTATT CACGACACAA TGCCGAAAGA CTTTTTAAAA AGCTGATACT TGACAAGATT 
TTGGATGAAG ACTTATATAT CAATGCCAAT GACCAGGCGA TCGCTTATGT GATGCTCGGA 
AATAAAGCCC AAACTGTACT AAATGGCAAT TTAAAGGTAG ACTTTATGGA AACAGAAAAT 
TCCAGCAGTG TGAAAAAACA AAAAGCGTTA GTAGCAAAAG TGTCTCAGAG GGAAGAGATG 
GTTAAAAAAT GTCTTGGAGA ACTTACAGAA GTCTGCAAAT CTCTGGGGAA AGTTTTTGGT 
GTCCATTACT TCAATATTTT TAATACCGTC ACTCTCAAGA AGCTTGCAGA ATCTTTATCT 
TCTGATCCTG AGGTTTTGCT TCAAATTGAT GGTGTTACTG AAGACAAACT GGAAAAATAT 
GGTGCGGAAG TGATTTCAGT ATTACAGAAA TACTCTGAAT GGACATCGCC AGCTGAAGAC 
AGTTCCCCAG GGATAAGCCT GTCCAGCAGC AGAGGCCCCG GAAGAAGTGC CGCTGAGGAG 
CTCGACGAGG AAATACCCGT ATCTTCCCAC TACTTTGCAA GTAAAACCAG AAATGAAAGG 
AAGAGGAAAA AGATGCCAGC CTCCCAAAGG TCTAAGAGGA GAAAAACTGC TTCCAGTGGT 
TCCAAGGCAA AGGGGGGGTC TGCCACATGT AGAAAGATAT CTTCCAAAAC GAAATCCTCC 
AGCATCATTG GATCCAGTTC AGCCTCACAT ACTTCTCAAG CGACATCAGG AGCCAATAGC 
AAATTGGGGA TTATGGCTCC ACCGAAGCCT ATAAATAGAC CGTTTCTTAA GCCTTCATAT 
GCATTCTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( XM_011521881.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520183.1 Homo sapiens BLM RecQ like helicase (BLM), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu56490
Clone ID Related Accession (Same CDS sequence)	XM_011521881.2
Accession Version	XM_011521881.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	Bloom syndrome protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGTG ATTCCTGCCC TACAGGGAAT TCTATGAAGG AGTTAAATTT TTCACACCTT 
CCCTCAAATT CTGTTTCTCC TGGGGACTGT TTACTGACTA CCACCCTAGG AAAGACAGGA 
TTCTCTGCCA CCAGGAAGAA TCTTTTTGAA AGGCCTTTAT TCAATACCCA TTTACAGAAG 
TCCTTTGTAA GTAGCAACTG GGCTGAAACA CCAAGACTAG GAAAAAAAAA TGAAAGCTCT 
TATTTCCCAG GAAATGTTCT CACAAGCACT GCTGTGAAAG ATCAGAATAA ACATACTGCT 
TCAATAAATG ACTTAGAAAG AGAAACCCAA CCTTCCTATG ATATTGATAA TTTTGACATA 
GATGACTTTG ATGATGATGA TGACTGGGAA GACATAATGC ATAATTTAGC AGCCAGCAAA 
TCTTCCACAG CTGCCTATCA ACCCATCAAG GAAGGTCGGC CAATTAAATC AGTATCAGAA 
AGACTTTCCT CAGCCAAGAC AGACTGTCTT CCAGTGTCAT CTACTGCTCA AAATATAAAC 
TTCTCAGAGT CAATTCAGAA TTATACTGAC AAGTCAGCAC AAAATTTAGC ATCCAGAAAT 
CTGAAACATG AGCGTTTCCA AAGTCTTAGT TTTCCTCATA CAAAGGAAAT GATGAAGATT 
TTTCATAAAA AATTTGGCCT GCATAATTTT AGAACTAATC AGCTAGAGGC GATCAATGCT 
GCACTGCTTG GTGAAGACTG TTTTATCCTG ATGCCGACTG GAGGTGGTAA GAGTTTGTGT 
TACCAGCTCC CTGCCTGTGT TTCTCCTGGG GTCACTGTTG TCATTTCTCC CTTGAGATCA 
CTTATCGTAG ATCAAGTCCA AAAGCTGACT TCCTTGGATA TTCCAGCTAC ATATCTGACA 
GGTGATAAGA CTGACTCAGA AGCTACAAAT ATTTACCTCC AGTTATCAAA AAAAGACCCA 
ATCATAAAAC TTCTATATGT CACTCCAGAA AAGATCTGTG CAAGTAACAG ACTCATTTCT 
ACTCTGGAGA ATCTCTATGA GAGGAAGCTC TTGGCACGTT TTGTTATTGA TGAAGCACAT 
TGTGTCAGTC AGTGGGGACA TGATTTTCGT CAAGATTACA AAAGAATGAA TATGCTTCGC 
CAGAAGTTTC CTTCTGTTCC GGTGATGGCT CTTACGGCCA CAGCTAATCC CAGGGTACAG 
AAGGACATCC TGACTCAGCT GAAGATTCTC AGACCTCAGG TGTTTAGCAT GAGCTTTAAC 
AGACATAATC TGAAATACTA TGTATTACCG AAAAAGCCTA AAAAGGTGGC ATTTGATTGC 
CTAGAATGGA TCAGAAAGCA CCACCCATAT GATTCAGGGA TAATTTACTG CCTCTCCAGG 
CGAGAATGTG ACACCATGGC TGACACGTTA CAGAGAGATG GGCTCGCTGC TCTTGCTTAC 
CATGCTGGCC TCAGTGATTC TGCCAGAGAT GAAGTGCAGC AGAAGTGGAT TAATCAGGAT 
GGCTGTCAGG TTATCTGTGC TACAATTGCA TTTGGAATGG GGATTGACAA ACCGGACGTG 
CGATTTGTGA TTCATGCATC TCTCCCTAAA TCTGTGGAGG GTTACTACCA AGAATCTGGC 
AGAGCTGGAA GAGATGGGGA AATATCTCAC TGCCTGCTTT TCTATACCTA TCATGATGTG 
ACCAGACTGA AAAGACTTAT AATGATGGAA AAAGATGGAA ACCATCATAC AAGAGAAACT 
CACTTCAATA ATTTGTATAG CATGGTACAT TACTGTGAAA ATATAACGGA ATGCAGGAGA 
ATACAGCTTT TGGCCTACTT TGGTGAAAAT GGATTTAATC CTGATTTTTG TAAGAAACAC 
CCAGATGTTT CTTGTGATAA TTGCTGTAAA ACAAAGGATT ATAAAACAAG AGATGTGACT 
GACGATGTGA AAAGTATTGT AAGATTTGTT CAAGAACATA GTTCATCACA AGGAATGAGA 
AATATAAAAC ATGTAGGTCC TTCTGGAAGA TTTACTATGA ATATGCTGGT CGACATTTTC 
TTGGGGAGTA AGAGTGCAAA AATCCAGTCA GGTATATTTG GAAAAGGATC TGCTTATTCA 
CGACACAATG CCGAAAGACT TTTTAAAAAG CTGATACTTG ACAAGATTTT GGATGAAGAC 
TTATATATCA ATGCCAATGA CCAGGCGATC GCTTATGTGA TGCTCGGAAA TAAAGCCCAA 
ACTGTACTAA ATGGCAATTT AAAGGTAGAC TTTATGGAAA CAGAAAATTC CAGCAGTGTG 
AAAAAACAAA AAGCGTTAGT AGCAAAAGTG TCTCAGAGGG AAGAGATGGT TAAAAAATGT 
CTTGGAGAAC TTACAGAAGT CTGCAAATCT CTGGGGAAAG TTTTTGGTGT CCATTACTTC 
AATATTTTTA ATACCGTCAC TCTCAAGAAG CTTGCAGAAT CTTTATCTTC TGATCCTGAG 
GTTTTGCTTC AAATTGATGG TGTTACTGAA GACAAACTGG AAAAATATGG TGCGGAAGTG 
ATTTCAGTAT TACAGAAATA CTCTGAATGG ACATCGCCAG CTGAAGACAG TTCCCCAGGG 
ATAAGCCTGT CCAGCAGCAG AGGCCCCGGA AGAAGTGCCG CTGAGGAGCT CGACGAGGAA 
ATACCCGTAT CTTCCCACTA CTTTGCAAGT AAAACCAGAA ATGAAAGGAA GAGGAAAAAG 
ATGCCAGCCT CCCAAAGGTC TAAGAGGAGA AAAACTGCTT CCAGTGGTTC CAAGGCAAAG 
GGGGGGTCTG CCACATGTAG AAAGATATCT TCCAAAACGA AATCCTCCAG CATCATTGGA 
TCCAGTTCAG CCTCACATAC TTCTCAAGCG ACATCAGGAG CCAATAGCAA ATTGGGGATT 
ATGGCTCCAC CGAAGCCTAT AAATAGACCG TTTCTTAAGC CTTCATATGC ATTCTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520183.1
CDS	468..3407
Translation	MEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	XM_011521881.2
Organism	Homo sapiens(human)
Definition	Homo sapiens BLM RecQ like helicase (BLM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521881.2

ORF Insert Sequence:

ATGGAGGGTG ATTCCTGCCC TACAGGGAAT TCTATGAAGG AGTTAAATTT TTCACACCTT 
CCCTCAAATT CTGTTTCTCC TGGGGACTGT TTACTGACTA CCACCCTAGG AAAGACAGGA 
TTCTCTGCCA CCAGGAAGAA TCTTTTTGAA AGGCCTTTAT TCAATACCCA TTTACAGAAG 
TCCTTTGTAA GTAGCAACTG GGCTGAAACA CCAAGACTAG GAAAAAAAAA TGAAAGCTCT 
TATTTCCCAG GAAATGTTCT CACAAGCACT GCTGTGAAAG ATCAGAATAA ACATACTGCT 
TCAATAAATG ACTTAGAAAG AGAAACCCAA CCTTCCTATG ATATTGATAA TTTTGACATA 
GATGACTTTG ATGATGATGA TGACTGGGAA GACATAATGC ATAATTTAGC AGCCAGCAAA 
TCTTCCACAG CTGCCTATCA ACCCATCAAG GAAGGTCGGC CAATTAAATC AGTATCAGAA 
AGACTTTCCT CAGCCAAGAC AGACTGTCTT CCAGTGTCAT CTACTGCTCA AAATATAAAC 
TTCTCAGAGT CAATTCAGAA TTATACTGAC AAGTCAGCAC AAAATTTAGC ATCCAGAAAT 
CTGAAACATG AGCGTTTCCA AAGTCTTAGT TTTCCTCATA CAAAGGAAAT GATGAAGATT 
TTTCATAAAA AATTTGGCCT GCATAATTTT AGAACTAATC AGCTAGAGGC GATCAATGCT 
GCACTGCTTG GTGAAGACTG TTTTATCCTG ATGCCGACTG GAGGTGGTAA GAGTTTGTGT 
TACCAGCTCC CTGCCTGTGT TTCTCCTGGG GTCACTGTTG TCATTTCTCC CTTGAGATCA 
CTTATCGTAG ATCAAGTCCA AAAGCTGACT TCCTTGGATA TTCCAGCTAC ATATCTGACA 
GGTGATAAGA CTGACTCAGA AGCTACAAAT ATTTACCTCC AGTTATCAAA AAAAGACCCA 
ATCATAAAAC TTCTATATGT CACTCCAGAA AAGATCTGTG CAAGTAACAG ACTCATTTCT 
ACTCTGGAGA ATCTCTATGA GAGGAAGCTC TTGGCACGTT TTGTTATTGA TGAAGCACAT 
TGTGTCAGTC AGTGGGGACA TGATTTTCGT CAAGATTACA AAAGAATGAA TATGCTTCGC 
CAGAAGTTTC CTTCTGTTCC GGTGATGGCT CTTACGGCCA CAGCTAATCC CAGGGTACAG 
AAGGACATCC TGACTCAGCT GAAGATTCTC AGACCTCAGG TGTTTAGCAT GAGCTTTAAC 
AGACATAATC TGAAATACTA TGTATTACCG AAAAAGCCTA AAAAGGTGGC ATTTGATTGC 
CTAGAATGGA TCAGAAAGCA CCACCCATAT GATTCAGGGA TAATTTACTG CCTCTCCAGG 
CGAGAATGTG ACACCATGGC TGACACGTTA CAGAGAGATG GGCTCGCTGC TCTTGCTTAC 
CATGCTGGCC TCAGTGATTC TGCCAGAGAT GAAGTGCAGC AGAAGTGGAT TAATCAGGAT 
GGCTGTCAGG TTATCTGTGC TACAATTGCA TTTGGAATGG GGATTGACAA ACCGGACGTG 
CGATTTGTGA TTCATGCATC TCTCCCTAAA TCTGTGGAGG GTTACTACCA AGAATCTGGC 
AGAGCTGGAA GAGATGGGGA AATATCTCAC TGCCTGCTTT TCTATACCTA TCATGATGTG 
ACCAGACTGA AAAGACTTAT AATGATGGAA AAAGATGGAA ACCATCATAC AAGAGAAACT 
CACTTCAATA ATTTGTATAG CATGGTACAT TACTGTGAAA ATATAACGGA ATGCAGGAGA 
ATACAGCTTT TGGCCTACTT TGGTGAAAAT GGATTTAATC CTGATTTTTG TAAGAAACAC 
CCAGATGTTT CTTGTGATAA TTGCTGTAAA ACAAAGGATT ATAAAACAAG AGATGTGACT 
GACGATGTGA AAAGTATTGT AAGATTTGTT CAAGAACATA GTTCATCACA AGGAATGAGA 
AATATAAAAC ATGTAGGTCC TTCTGGAAGA TTTACTATGA ATATGCTGGT CGACATTTTC 
TTGGGGAGTA AGAGTGCAAA AATCCAGTCA GGTATATTTG GAAAAGGATC TGCTTATTCA 
CGACACAATG CCGAAAGACT TTTTAAAAAG CTGATACTTG ACAAGATTTT GGATGAAGAC 
TTATATATCA ATGCCAATGA CCAGGCGATC GCTTATGTGA TGCTCGGAAA TAAAGCCCAA 
ACTGTACTAA ATGGCAATTT AAAGGTAGAC TTTATGGAAA CAGAAAATTC CAGCAGTGTG 
AAAAAACAAA AAGCGTTAGT AGCAAAAGTG TCTCAGAGGG AAGAGATGGT TAAAAAATGT 
CTTGGAGAAC TTACAGAAGT CTGCAAATCT CTGGGGAAAG TTTTTGGTGT CCATTACTTC 
AATATTTTTA ATACCGTCAC TCTCAAGAAG CTTGCAGAAT CTTTATCTTC TGATCCTGAG 
GTTTTGCTTC AAATTGATGG TGTTACTGAA GACAAACTGG AAAAATATGG TGCGGAAGTG 
ATTTCAGTAT TACAGAAATA CTCTGAATGG ACATCGCCAG CTGAAGACAG TTCCCCAGGG 
ATAAGCCTGT CCAGCAGCAG AGGCCCCGGA AGAAGTGCCG CTGAGGAGCT CGACGAGGAA 
ATACCCGTAT CTTCCCACTA CTTTGCAAGT AAAACCAGAA ATGAAAGGAA GAGGAAAAAG 
ATGCCAGCCT CCCAAAGGTC TAAGAGGAGA AAAACTGCTT CCAGTGGTTC CAAGGCAAAG 
GGGGGGTCTG CCACATGTAG AAAGATATCT TCCAAAACGA AATCCTCCAG CATCATTGGA 
TCCAGTTCAG CCTCACATAC TTCTCAAGCG ACATCAGGAG CCAATAGCAA ATTGGGGATT 
ATGGCTCCAC CGAAGCCTAT AAATAGACCG TTTCTTAAGC CTTCATATGC ATTCTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( XM_011521882.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520184.1 Homo sapiens Bloom syndrome RecQ like helicase (BLM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu56491
Clone ID Related Accession (Same CDS sequence)	XM_011521882.2 , XM_011521882.3
Accession Version	XM_011521882.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	Bloom syndrome protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTAATTG TACAACCATC CCCAATATCT 
AAGTTTACAG CATTTTTATC ACCCCAGCGA GAAACCTGGT ACCCATTAGC AGCTGCTCCG 
CATCTTCTCC CTCTTGTTTG TGCCCGGCTT CTTTCACTGA GCGTAGTTTT CAAGGGTCAT 
CCATGTTGTA GCATTGGTCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520184.1
CDS	106..2829
Translation	MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVIVQPSPISKFTAFLSPQRETWYPLAAAPHLLPLVCARLLSLSVVFKGHPCCSIGQ

Target ORF information:

RefSeq Version	XM_011521882.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Bloom syndrome RecQ like helicase (BLM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521882.2

ORF Insert Sequence:

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTAATTG TACAACCATC CCCAATATCT 
AAGTTTACAG CATTTTTATC ACCCCAGCGA GAAACCTGGT ACCCATTAGC AGCTGCTCCG 
CATCTTCTCC CTCTTGTTTG TGCCCGGCTT CTTTCACTGA GCGTAGTTTT CAAGGGTCAT 
CCATGTTGTA GCATTGGTCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( XM_011521882.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520184.1 Homo sapiens BLM RecQ like helicase (BLM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu56491
Clone ID Related Accession (Same CDS sequence)	XM_011521882.2 , XM_011521882.3
Accession Version	XM_011521882.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	Bloom syndrome protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521882.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTAATTG TACAACCATC CCCAATATCT 
AAGTTTACAG CATTTTTATC ACCCCAGCGA GAAACCTGGT ACCCATTAGC AGCTGCTCCG 
CATCTTCTCC CTCTTGTTTG TGCCCGGCTT CTTTCACTGA GCGTAGTTTT CAAGGGTCAT 
CCATGTTGTA GCATTGGTCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520184.1
CDS	48..2771
Translation	MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVIVQPSPISKFTAFLSPQRETWYPLAAAPHLLPLVCARLLSLSVVFKGHPCCSIGQ

Target ORF information:

RefSeq Version	XM_011521882.3
Organism	Homo sapiens(human)
Definition	Homo sapiens BLM RecQ like helicase (BLM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521882.3

ORF Insert Sequence:

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTAATTG TACAACCATC CCCAATATCT 
AAGTTTACAG CATTTTTATC ACCCCAGCGA GAAACCTGGT ACCCATTAGC AGCTGCTCCG 
CATCTTCTCC CTCTTGTTTG TGCCCGGCTT CTTTCACTGA GCGTAGTTTT CAAGGGTCAT 
CCATGTTGTA GCATTGGTCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( NM_001287246.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274175.1 Homo sapiens BLM RecQ like helicase (BLM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20445
Clone ID Related Accession (Same CDS sequence)	NM_001287246.1 , NM_001287246.2 , NM_000057.4 , NM_000057.3
Accession Version	NM_001287246.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	Bloom syndrome protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BP363946.1, BC143288.1, BC115032.1, DB520927.1 and AC124248.6. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143288.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT 
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA 
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA 
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT 
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT 
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT 
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT 
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA 
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC 
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG 
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT 
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT 
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA 
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG 
AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC 
AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT 
ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA 
CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA 
CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA 
GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT 
TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG 
CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA 
GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC 
CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC 
GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA 
GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG 
TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT 
TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT 
CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274175.1
CDS	229..4482
Translation	MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	NM_001287246.1
Organism	Homo sapiens(human)
Definition	Homo sapiens BLM RecQ like helicase (BLM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287246.1

ORF Insert Sequence:

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT 
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA 
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA 
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT 
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT 
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT 
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT 
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA 
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC 
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG 
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT 
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT 
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA 
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG 
AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC 
AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT 
ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA 
CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA 
CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA 
GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT 
TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG 
CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA 
GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC 
CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC 
GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA 
GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG 
TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT 
TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT 
CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( NM_001287246.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274175.1 Homo sapiens BLM RecQ like helicase (BLM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20445
Clone ID Related Accession (Same CDS sequence)	NM_001287246.1 , NM_001287246.2 , NM_000057.4 , NM_000057.3
Accession Version	NM_001287246.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	Bloom syndrome protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BP363946.1, BC143288.1, BC115032.1, DB520927.1 and AC124248.6. On May 31, 2019 this sequence version replaced NM_001287246.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143288.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT 
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA 
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA 
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT 
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT 
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT 
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT 
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA 
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC 
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG 
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT 
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT 
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA 
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG 
AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC 
AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT 
ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA 
CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA 
CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA 
GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT 
TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG 
CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA 
GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC 
CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC 
GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA 
GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG 
TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT 
TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT 
CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274175.1
CDS	210..4463
Translation	MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	NM_001287246.2
Organism	Homo sapiens(human)
Definition	Homo sapiens BLM RecQ like helicase (BLM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287246.2

ORF Insert Sequence:

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT 
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA 
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA 
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT 
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT 
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT 
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT 
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA 
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC 
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG 
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT 
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT 
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA 
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG 
AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC 
AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT 
ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA 
CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA 
CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA 
GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT 
TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG 
CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA 
GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC 
CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC 
GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA 
GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG 
TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT 
TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT 
CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( NM_000057.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000048.1 Homo sapiens BLM RecQ like helicase (BLM), transcript variant 1, mRNA.

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CloneID	OHu20445
Clone ID Related Accession (Same CDS sequence)	NM_001287246.1 , NM_001287246.2 , NM_000057.4 , NM_000057.3
Accession Version	NM_000057.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	Bloom syndrome protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, U39817.1, BC115030.1, BC034480.1 and AC124248.6. On Nov 22, 2018 this sequence version replaced NM_000057.3. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034480.1, U39817.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000355112.8/ ENSP00000347232.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT 
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA 
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA 
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT 
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT 
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT 
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT 
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA 
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC 
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG 
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT 
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT 
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA 
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG 
AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC 
AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT 
ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA 
CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA 
CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA 
GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT 
TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG 
CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA 
GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC 
CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC 
GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA 
GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG 
TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT 
TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT 
CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000048.1
CDS	100..4353
Translation	MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	NM_000057.4
Organism	Homo sapiens(human)
Definition	Homo sapiens BLM RecQ like helicase (BLM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000057.4

ORF Insert Sequence:

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT 
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA 
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA 
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT 
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT 
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT 
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT 
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA 
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC 
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG 
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT 
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT 
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA 
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG 
AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC 
AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT 
ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA 
CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA 
CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA 
GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT 
TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG 
CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA 
GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC 
CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC 
GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA 
GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG 
TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT 
TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT 
CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( NM_000057.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000048.1 Homo sapiens Bloom syndrome RecQ like helicase (BLM), transcript variant 1, mRNA.

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CloneID	OHu20445
Clone ID Related Accession (Same CDS sequence)	NM_001287246.1 , NM_001287246.2 , NM_000057.4 , NM_000057.3
Accession Version	NM_000057.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	Bloom syndrome protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, U39817.1, BC115030.1, BC034480.1 and AC124248.6. On Dec 11, 2013 this sequence version replaced NM_000057.2. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034480.1, U39817.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT 
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA 
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA 
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT 
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT 
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT 
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT 
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA 
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC 
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG 
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT 
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT 
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA 
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG 
AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC 
AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT 
ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA 
CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA 
CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA 
GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT 
TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG 
CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA 
GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC 
CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC 
GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA 
GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG 
TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT 
TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT 
CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000048.1
CDS	119..4372
Translation	MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	NM_000057.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Bloom syndrome RecQ like helicase (BLM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000057.3

ORF Insert Sequence:

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT 
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA 
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA 
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT 
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT 
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT 
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT 
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA 
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC 
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG 
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT 
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT 
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA 
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG 
AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC 
AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT 
ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA 
CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA 
CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA 
GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT 
TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG 
CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA 
GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC 
CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC 
GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA 
GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG 
TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT 
TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT 
CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( NM_001287248.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274177.1 Homo sapiens BLM RecQ like helicase (BLM), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu20947
Clone ID Related Accession (Same CDS sequence)	NM_001287248.2 , NM_001287248.1
Accession Version	NM_001287248.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	Bloom syndrome protein isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, BC143280.1, AC021422.25, DA122319.1, BC115032.1, DB520927.1 and AC124248.6. On May 31, 2019 this sequence version replaced NM_001287248.1. Transcript Variant: This variant (4) uses an alternate splice site in a 5' exon and uses a downstream translation start compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143280.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCACA TCTGTAAATT AATTGATACT ATTCCTGATG ATAAACTGAA ACTTTTGGAT 
TGTGGGAACG AACTGCTTCA GCAGCGGAAC ATAAGAAGGA AACTTCTAAC GGAAGTAGAT 
TTTAATAAAA GTGATGCCAG TCTTCTTGGC TCATTGTGGA GATACAGGCC TGATTCACTT 
GATGGCCCTA TGGAGGGTGA TTCCTGCCCT ACAGGGAATT CTATGAAGGA GTTAAATTTT 
TCACACCTTC CCTCAAATTC TGTTTCTCCT GGGGACTGTT TACTGACTAC CACCCTAGGA 
AAGACAGGAT TCTCTGCCAC CAGGAAGAAT CTTTTTGAAA GGCCTTTATT CAATACCCAT 
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGACTAGG AAAAAAAAAT 
GAAAGCTCTT ATTTCCCAGG AAATGTTCTC ACAAGCACTG CTGTGAAAGA TCAGAATAAA 
CATACTGCTT CAATAAATGA CTTAGAAAGA GAAACCCAAC CTTCCTATGA TATTGATAAT 
TTTGACATAG ATGACTTTGA TGATGATGAT GACTGGGAAG ACATAATGCA TAATTTAGCA 
GCCAGCAAAT CTTCCACAGC TGCCTATCAA CCCATCAAGG AAGGTCGGCC AATTAAATCA 
GTATCAGAAA GACTTTCCTC AGCCAAGACA GACTGTCTTC CAGTGTCATC TACTGCTCAA 
AATATAAACT TCTCAGAGTC AATTCAGAAT TATACTGACA AGTCAGCACA AAATTTAGCA 
TCCAGAAATC TGAAACATGA GCGTTTCCAA AGTCTTAGTT TTCCTCATAC AAAGGAAATG 
ATGAAGATTT TTCATAAAAA ATTTGGCCTG CATAATTTTA GAACTAATCA GCTAGAGGCG 
ATCAATGCTG CACTGCTTGG TGAAGACTGT TTTATCCTGA TGCCGACTGG AGGTGGTAAG 
AGTTTGTGTT ACCAGCTCCC TGCCTGTGTT TCTCCTGGGG TCACTGTTGT CATTTCTCCC 
TTGAGATCAC TTATCGTAGA TCAAGTCCAA AAGCTGACTT CCTTGGATAT TCCAGCTACA 
TATCTGACAG GTGATAAGAC TGACTCAGAA GCTACAAATA TTTACCTCCA GTTATCAAAA 
AAAGACCCAA TCATAAAACT TCTATATGTC ACTCCAGAAA AGATCTGTGC AAGTAACAGA 
CTCATTTCTA CTCTGGAGAA TCTCTATGAG AGGAAGCTCT TGGCACGTTT TGTTATTGAT 
GAAGCACATT GTGTCAGTCA GTGGGGACAT GATTTTCGTC AAGATTACAA AAGAATGAAT 
ATGCTTCGCC AGAAGTTTCC TTCTGTTCCG GTGATGGCTC TTACGGCCAC AGCTAATCCC 
AGGGTACAGA AGGACATCCT GACTCAGCTG AAGATTCTCA GACCTCAGGT GTTTAGCATG 
AGCTTTAACA GACATAATCT GAAATACTAT GTATTACCGA AAAAGCCTAA AAAGGTGGCA 
TTTGATTGCC TAGAATGGAT CAGAAAGCAC CACCCATATG ATTCAGGGAT AATTTACTGC 
CTCTCCAGGC GAGAATGTGA CACCATGGCT GACACGTTAC AGAGAGATGG GCTCGCTGCT 
CTTGCTTACC ATGCTGGCCT CAGTGATTCT GCCAGAGATG AAGTGCAGCA GAAGTGGATT 
AATCAGGATG GCTGTCAGGT TATCTGTGCT ACAATTGCAT TTGGAATGGG GATTGACAAA 
CCGGACGTGC GATTTGTGAT TCATGCATCT CTCCCTAAAT CTGTGGAGGG TTACTACCAA 
GAATCTGGCA GAGCTGGAAG AGATGGGGAA ATATCTCACT GCCTGCTTTT CTATACCTAT 
CATGATGTGA CCAGACTGAA AAGACTTATA ATGATGGAAA AAGATGGAAA CCATCATACA 
AGAGAAACTC ACTTCAATAA TTTGTATAGC ATGGTACATT ACTGTGAAAA TATAACGGAA 
TGCAGGAGAA TACAGCTTTT GGCCTACTTT GGTGAAAATG GATTTAATCC TGATTTTTGT 
AAGAAACACC CAGATGTTTC TTGTGATAAT TGCTGTAAAA CAAAGGATTA TAAAACAAGA 
GATGTGACTG ACGATGTGAA AAGTATTGTA AGATTTGTTC AAGAACATAG TTCATCACAA 
GGAATGAGAA ATATAAAACA TGTAGGTCCT TCTGGAAGAT TTACTATGAA TATGCTGGTC 
GACATTTTCT TGGGGAGTAA GAGTGCAAAA ATCCAGTCAG GTATATTTGG AAAAGGATCT 
GCTTATTCAC GACACAATGC CGAAAGACTT TTTAAAAAGC TGATACTTGA CAAGATTTTG 
GATGAAGACT TATATATCAA TGCCAATGAC CAGGCGATCG CTTATGTGAT GCTCGGAAAT 
AAAGCCCAAA CTGTACTAAA TGGCAATTTA AAGGTAGACT TTATGGAAAC AGAAAATTCC 
AGCAGTGTGA AAAAACAAAA AGCGTTAGTA GCAAAAGTGT CTCAGAGGGA AGAGATGGTT 
AAAAAATGTC TTGGAGAACT TACAGAAGTC TGCAAATCTC TGGGGAAAGT TTTTGGTGTC 
CATTACTTCA ATATTTTTAA TACCGTCACT CTCAAGAAGC TTGCAGAATC TTTATCTTCT 
GATCCTGAGG TTTTGCTTCA AATTGATGGT GTTACTGAAG ACAAACTGGA AAAATATGGT 
GCGGAAGTGA TTTCAGTATT ACAGAAATAC TCTGAATGGA CATCGCCAGC TGAAGACAGT 
TCCCCAGGGA TAAGCCTGTC CAGCAGCAGA GGCCCCGGAA GAAGTGCCGC TGAGGAGCTC 
GACGAGGAAA TACCCGTATC TTCCCACTAC TTTGCAAGTA AAACCAGAAA TGAAAGGAAG 
AGGAAAAAGA TGCCAGCCTC CCAAAGGTCT AAGAGGAGAA AAACTGCTTC CAGTGGTTCC 
AAGGCAAAGG GGGGGTCTGC CACATGTAGA AAGATATCTT CCAAAACGAA ATCCTCCAGC 
ATCATTGGAT CCAGTTCAGC CTCACATACT TCTCAAGCGA CATCAGGAGC CAATAGCAAA 
TTGGGGATTA TGGCTCCACC GAAGCCTATA AATAGACCGT TTCTTAAGCC TTCATATGCA 
TTCTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274177.1
CDS	1391..4519
Translation	MEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	NM_001287248.2
Organism	Homo sapiens(human)
Definition	Homo sapiens BLM RecQ like helicase (BLM), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287248.2

ORF Insert Sequence:

ATGGAGCACA TCTGTAAATT AATTGATACT ATTCCTGATG ATAAACTGAA ACTTTTGGAT 
TGTGGGAACG AACTGCTTCA GCAGCGGAAC ATAAGAAGGA AACTTCTAAC GGAAGTAGAT 
TTTAATAAAA GTGATGCCAG TCTTCTTGGC TCATTGTGGA GATACAGGCC TGATTCACTT 
GATGGCCCTA TGGAGGGTGA TTCCTGCCCT ACAGGGAATT CTATGAAGGA GTTAAATTTT 
TCACACCTTC CCTCAAATTC TGTTTCTCCT GGGGACTGTT TACTGACTAC CACCCTAGGA 
AAGACAGGAT TCTCTGCCAC CAGGAAGAAT CTTTTTGAAA GGCCTTTATT CAATACCCAT 
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGACTAGG AAAAAAAAAT 
GAAAGCTCTT ATTTCCCAGG AAATGTTCTC ACAAGCACTG CTGTGAAAGA TCAGAATAAA 
CATACTGCTT CAATAAATGA CTTAGAAAGA GAAACCCAAC CTTCCTATGA TATTGATAAT 
TTTGACATAG ATGACTTTGA TGATGATGAT GACTGGGAAG ACATAATGCA TAATTTAGCA 
GCCAGCAAAT CTTCCACAGC TGCCTATCAA CCCATCAAGG AAGGTCGGCC AATTAAATCA 
GTATCAGAAA GACTTTCCTC AGCCAAGACA GACTGTCTTC CAGTGTCATC TACTGCTCAA 
AATATAAACT TCTCAGAGTC AATTCAGAAT TATACTGACA AGTCAGCACA AAATTTAGCA 
TCCAGAAATC TGAAACATGA GCGTTTCCAA AGTCTTAGTT TTCCTCATAC AAAGGAAATG 
ATGAAGATTT TTCATAAAAA ATTTGGCCTG CATAATTTTA GAACTAATCA GCTAGAGGCG 
ATCAATGCTG CACTGCTTGG TGAAGACTGT TTTATCCTGA TGCCGACTGG AGGTGGTAAG 
AGTTTGTGTT ACCAGCTCCC TGCCTGTGTT TCTCCTGGGG TCACTGTTGT CATTTCTCCC 
TTGAGATCAC TTATCGTAGA TCAAGTCCAA AAGCTGACTT CCTTGGATAT TCCAGCTACA 
TATCTGACAG GTGATAAGAC TGACTCAGAA GCTACAAATA TTTACCTCCA GTTATCAAAA 
AAAGACCCAA TCATAAAACT TCTATATGTC ACTCCAGAAA AGATCTGTGC AAGTAACAGA 
CTCATTTCTA CTCTGGAGAA TCTCTATGAG AGGAAGCTCT TGGCACGTTT TGTTATTGAT 
GAAGCACATT GTGTCAGTCA GTGGGGACAT GATTTTCGTC AAGATTACAA AAGAATGAAT 
ATGCTTCGCC AGAAGTTTCC TTCTGTTCCG GTGATGGCTC TTACGGCCAC AGCTAATCCC 
AGGGTACAGA AGGACATCCT GACTCAGCTG AAGATTCTCA GACCTCAGGT GTTTAGCATG 
AGCTTTAACA GACATAATCT GAAATACTAT GTATTACCGA AAAAGCCTAA AAAGGTGGCA 
TTTGATTGCC TAGAATGGAT CAGAAAGCAC CACCCATATG ATTCAGGGAT AATTTACTGC 
CTCTCCAGGC GAGAATGTGA CACCATGGCT GACACGTTAC AGAGAGATGG GCTCGCTGCT 
CTTGCTTACC ATGCTGGCCT CAGTGATTCT GCCAGAGATG AAGTGCAGCA GAAGTGGATT 
AATCAGGATG GCTGTCAGGT TATCTGTGCT ACAATTGCAT TTGGAATGGG GATTGACAAA 
CCGGACGTGC GATTTGTGAT TCATGCATCT CTCCCTAAAT CTGTGGAGGG TTACTACCAA 
GAATCTGGCA GAGCTGGAAG AGATGGGGAA ATATCTCACT GCCTGCTTTT CTATACCTAT 
CATGATGTGA CCAGACTGAA AAGACTTATA ATGATGGAAA AAGATGGAAA CCATCATACA 
AGAGAAACTC ACTTCAATAA TTTGTATAGC ATGGTACATT ACTGTGAAAA TATAACGGAA 
TGCAGGAGAA TACAGCTTTT GGCCTACTTT GGTGAAAATG GATTTAATCC TGATTTTTGT 
AAGAAACACC CAGATGTTTC TTGTGATAAT TGCTGTAAAA CAAAGGATTA TAAAACAAGA 
GATGTGACTG ACGATGTGAA AAGTATTGTA AGATTTGTTC AAGAACATAG TTCATCACAA 
GGAATGAGAA ATATAAAACA TGTAGGTCCT TCTGGAAGAT TTACTATGAA TATGCTGGTC 
GACATTTTCT TGGGGAGTAA GAGTGCAAAA ATCCAGTCAG GTATATTTGG AAAAGGATCT 
GCTTATTCAC GACACAATGC CGAAAGACTT TTTAAAAAGC TGATACTTGA CAAGATTTTG 
GATGAAGACT TATATATCAA TGCCAATGAC CAGGCGATCG CTTATGTGAT GCTCGGAAAT 
AAAGCCCAAA CTGTACTAAA TGGCAATTTA AAGGTAGACT TTATGGAAAC AGAAAATTCC 
AGCAGTGTGA AAAAACAAAA AGCGTTAGTA GCAAAAGTGT CTCAGAGGGA AGAGATGGTT 
AAAAAATGTC TTGGAGAACT TACAGAAGTC TGCAAATCTC TGGGGAAAGT TTTTGGTGTC 
CATTACTTCA ATATTTTTAA TACCGTCACT CTCAAGAAGC TTGCAGAATC TTTATCTTCT 
GATCCTGAGG TTTTGCTTCA AATTGATGGT GTTACTGAAG ACAAACTGGA AAAATATGGT 
GCGGAAGTGA TTTCAGTATT ACAGAAATAC TCTGAATGGA CATCGCCAGC TGAAGACAGT 
TCCCCAGGGA TAAGCCTGTC CAGCAGCAGA GGCCCCGGAA GAAGTGCCGC TGAGGAGCTC 
GACGAGGAAA TACCCGTATC TTCCCACTAC TTTGCAAGTA AAACCAGAAA TGAAAGGAAG 
AGGAAAAAGA TGCCAGCCTC CCAAAGGTCT AAGAGGAGAA AAACTGCTTC CAGTGGTTCC 
AAGGCAAAGG GGGGGTCTGC CACATGTAGA AAGATATCTT CCAAAACGAA ATCCTCCAGC 
ATCATTGGAT CCAGTTCAGC CTCACATACT TCTCAAGCGA CATCAGGAGC CAATAGCAAA 
TTGGGGATTA TGGCTCCACC GAAGCCTATA AATAGACCGT TTCTTAAGCC TTCATATGCA 
TTCTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( NM_001287248.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274177.1 Homo sapiens BLM RecQ like helicase (BLM), transcript variant 4, mRNA.

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CloneID	OHu20947
Clone ID Related Accession (Same CDS sequence)	NM_001287248.2 , NM_001287248.1
Accession Version	NM_001287248.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	Bloom syndrome protein isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, BC143280.1, AC021422.25, DA122319.1, BC115032.1, DB520927.1 and AC124248.6. Transcript Variant: This variant (4) uses an alternate splice site in a 5' exon and uses a downstream translation start compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143280.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCACA TCTGTAAATT AATTGATACT ATTCCTGATG ATAAACTGAA ACTTTTGGAT 
TGTGGGAACG AACTGCTTCA GCAGCGGAAC ATAAGAAGGA AACTTCTAAC GGAAGTAGAT 
TTTAATAAAA GTGATGCCAG TCTTCTTGGC TCATTGTGGA GATACAGGCC TGATTCACTT 
GATGGCCCTA TGGAGGGTGA TTCCTGCCCT ACAGGGAATT CTATGAAGGA GTTAAATTTT 
TCACACCTTC CCTCAAATTC TGTTTCTCCT GGGGACTGTT TACTGACTAC CACCCTAGGA 
AAGACAGGAT TCTCTGCCAC CAGGAAGAAT CTTTTTGAAA GGCCTTTATT CAATACCCAT 
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGACTAGG AAAAAAAAAT 
GAAAGCTCTT ATTTCCCAGG AAATGTTCTC ACAAGCACTG CTGTGAAAGA TCAGAATAAA 
CATACTGCTT CAATAAATGA CTTAGAAAGA GAAACCCAAC CTTCCTATGA TATTGATAAT 
TTTGACATAG ATGACTTTGA TGATGATGAT GACTGGGAAG ACATAATGCA TAATTTAGCA 
GCCAGCAAAT CTTCCACAGC TGCCTATCAA CCCATCAAGG AAGGTCGGCC AATTAAATCA 
GTATCAGAAA GACTTTCCTC AGCCAAGACA GACTGTCTTC CAGTGTCATC TACTGCTCAA 
AATATAAACT TCTCAGAGTC AATTCAGAAT TATACTGACA AGTCAGCACA AAATTTAGCA 
TCCAGAAATC TGAAACATGA GCGTTTCCAA AGTCTTAGTT TTCCTCATAC AAAGGAAATG 
ATGAAGATTT TTCATAAAAA ATTTGGCCTG CATAATTTTA GAACTAATCA GCTAGAGGCG 
ATCAATGCTG CACTGCTTGG TGAAGACTGT TTTATCCTGA TGCCGACTGG AGGTGGTAAG 
AGTTTGTGTT ACCAGCTCCC TGCCTGTGTT TCTCCTGGGG TCACTGTTGT CATTTCTCCC 
TTGAGATCAC TTATCGTAGA TCAAGTCCAA AAGCTGACTT CCTTGGATAT TCCAGCTACA 
TATCTGACAG GTGATAAGAC TGACTCAGAA GCTACAAATA TTTACCTCCA GTTATCAAAA 
AAAGACCCAA TCATAAAACT TCTATATGTC ACTCCAGAAA AGATCTGTGC AAGTAACAGA 
CTCATTTCTA CTCTGGAGAA TCTCTATGAG AGGAAGCTCT TGGCACGTTT TGTTATTGAT 
GAAGCACATT GTGTCAGTCA GTGGGGACAT GATTTTCGTC AAGATTACAA AAGAATGAAT 
ATGCTTCGCC AGAAGTTTCC TTCTGTTCCG GTGATGGCTC TTACGGCCAC AGCTAATCCC 
AGGGTACAGA AGGACATCCT GACTCAGCTG AAGATTCTCA GACCTCAGGT GTTTAGCATG 
AGCTTTAACA GACATAATCT GAAATACTAT GTATTACCGA AAAAGCCTAA AAAGGTGGCA 
TTTGATTGCC TAGAATGGAT CAGAAAGCAC CACCCATATG ATTCAGGGAT AATTTACTGC 
CTCTCCAGGC GAGAATGTGA CACCATGGCT GACACGTTAC AGAGAGATGG GCTCGCTGCT 
CTTGCTTACC ATGCTGGCCT CAGTGATTCT GCCAGAGATG AAGTGCAGCA GAAGTGGATT 
AATCAGGATG GCTGTCAGGT TATCTGTGCT ACAATTGCAT TTGGAATGGG GATTGACAAA 
CCGGACGTGC GATTTGTGAT TCATGCATCT CTCCCTAAAT CTGTGGAGGG TTACTACCAA 
GAATCTGGCA GAGCTGGAAG AGATGGGGAA ATATCTCACT GCCTGCTTTT CTATACCTAT 
CATGATGTGA CCAGACTGAA AAGACTTATA ATGATGGAAA AAGATGGAAA CCATCATACA 
AGAGAAACTC ACTTCAATAA TTTGTATAGC ATGGTACATT ACTGTGAAAA TATAACGGAA 
TGCAGGAGAA TACAGCTTTT GGCCTACTTT GGTGAAAATG GATTTAATCC TGATTTTTGT 
AAGAAACACC CAGATGTTTC TTGTGATAAT TGCTGTAAAA CAAAGGATTA TAAAACAAGA 
GATGTGACTG ACGATGTGAA AAGTATTGTA AGATTTGTTC AAGAACATAG TTCATCACAA 
GGAATGAGAA ATATAAAACA TGTAGGTCCT TCTGGAAGAT TTACTATGAA TATGCTGGTC 
GACATTTTCT TGGGGAGTAA GAGTGCAAAA ATCCAGTCAG GTATATTTGG AAAAGGATCT 
GCTTATTCAC GACACAATGC CGAAAGACTT TTTAAAAAGC TGATACTTGA CAAGATTTTG 
GATGAAGACT TATATATCAA TGCCAATGAC CAGGCGATCG CTTATGTGAT GCTCGGAAAT 
AAAGCCCAAA CTGTACTAAA TGGCAATTTA AAGGTAGACT TTATGGAAAC AGAAAATTCC 
AGCAGTGTGA AAAAACAAAA AGCGTTAGTA GCAAAAGTGT CTCAGAGGGA AGAGATGGTT 
AAAAAATGTC TTGGAGAACT TACAGAAGTC TGCAAATCTC TGGGGAAAGT TTTTGGTGTC 
CATTACTTCA ATATTTTTAA TACCGTCACT CTCAAGAAGC TTGCAGAATC TTTATCTTCT 
GATCCTGAGG TTTTGCTTCA AATTGATGGT GTTACTGAAG ACAAACTGGA AAAATATGGT 
GCGGAAGTGA TTTCAGTATT ACAGAAATAC TCTGAATGGA CATCGCCAGC TGAAGACAGT 
TCCCCAGGGA TAAGCCTGTC CAGCAGCAGA GGCCCCGGAA GAAGTGCCGC TGAGGAGCTC 
GACGAGGAAA TACCCGTATC TTCCCACTAC TTTGCAAGTA AAACCAGAAA TGAAAGGAAG 
AGGAAAAAGA TGCCAGCCTC CCAAAGGTCT AAGAGGAGAA AAACTGCTTC CAGTGGTTCC 
AAGGCAAAGG GGGGGTCTGC CACATGTAGA AAGATATCTT CCAAAACGAA ATCCTCCAGC 
ATCATTGGAT CCAGTTCAGC CTCACATACT TCTCAAGCGA CATCAGGAGC CAATAGCAAA 
TTGGGGATTA TGGCTCCACC GAAGCCTATA AATAGACCGT TTCTTAAGCC TTCATATGCA 
TTCTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274177.1
CDS	1410..4538
Translation	MEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	NM_001287248.1
Organism	Homo sapiens(human)
Definition	Homo sapiens BLM RecQ like helicase (BLM), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287248.1

ORF Insert Sequence:

ATGGAGCACA TCTGTAAATT AATTGATACT ATTCCTGATG ATAAACTGAA ACTTTTGGAT 
TGTGGGAACG AACTGCTTCA GCAGCGGAAC ATAAGAAGGA AACTTCTAAC GGAAGTAGAT 
TTTAATAAAA GTGATGCCAG TCTTCTTGGC TCATTGTGGA GATACAGGCC TGATTCACTT 
GATGGCCCTA TGGAGGGTGA TTCCTGCCCT ACAGGGAATT CTATGAAGGA GTTAAATTTT 
TCACACCTTC CCTCAAATTC TGTTTCTCCT GGGGACTGTT TACTGACTAC CACCCTAGGA 
AAGACAGGAT TCTCTGCCAC CAGGAAGAAT CTTTTTGAAA GGCCTTTATT CAATACCCAT 
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGACTAGG AAAAAAAAAT 
GAAAGCTCTT ATTTCCCAGG AAATGTTCTC ACAAGCACTG CTGTGAAAGA TCAGAATAAA 
CATACTGCTT CAATAAATGA CTTAGAAAGA GAAACCCAAC CTTCCTATGA TATTGATAAT 
TTTGACATAG ATGACTTTGA TGATGATGAT GACTGGGAAG ACATAATGCA TAATTTAGCA 
GCCAGCAAAT CTTCCACAGC TGCCTATCAA CCCATCAAGG AAGGTCGGCC AATTAAATCA 
GTATCAGAAA GACTTTCCTC AGCCAAGACA GACTGTCTTC CAGTGTCATC TACTGCTCAA 
AATATAAACT TCTCAGAGTC AATTCAGAAT TATACTGACA AGTCAGCACA AAATTTAGCA 
TCCAGAAATC TGAAACATGA GCGTTTCCAA AGTCTTAGTT TTCCTCATAC AAAGGAAATG 
ATGAAGATTT TTCATAAAAA ATTTGGCCTG CATAATTTTA GAACTAATCA GCTAGAGGCG 
ATCAATGCTG CACTGCTTGG TGAAGACTGT TTTATCCTGA TGCCGACTGG AGGTGGTAAG 
AGTTTGTGTT ACCAGCTCCC TGCCTGTGTT TCTCCTGGGG TCACTGTTGT CATTTCTCCC 
TTGAGATCAC TTATCGTAGA TCAAGTCCAA AAGCTGACTT CCTTGGATAT TCCAGCTACA 
TATCTGACAG GTGATAAGAC TGACTCAGAA GCTACAAATA TTTACCTCCA GTTATCAAAA 
AAAGACCCAA TCATAAAACT TCTATATGTC ACTCCAGAAA AGATCTGTGC AAGTAACAGA 
CTCATTTCTA CTCTGGAGAA TCTCTATGAG AGGAAGCTCT TGGCACGTTT TGTTATTGAT 
GAAGCACATT GTGTCAGTCA GTGGGGACAT GATTTTCGTC AAGATTACAA AAGAATGAAT 
ATGCTTCGCC AGAAGTTTCC TTCTGTTCCG GTGATGGCTC TTACGGCCAC AGCTAATCCC 
AGGGTACAGA AGGACATCCT GACTCAGCTG AAGATTCTCA GACCTCAGGT GTTTAGCATG 
AGCTTTAACA GACATAATCT GAAATACTAT GTATTACCGA AAAAGCCTAA AAAGGTGGCA 
TTTGATTGCC TAGAATGGAT CAGAAAGCAC CACCCATATG ATTCAGGGAT AATTTACTGC 
CTCTCCAGGC GAGAATGTGA CACCATGGCT GACACGTTAC AGAGAGATGG GCTCGCTGCT 
CTTGCTTACC ATGCTGGCCT CAGTGATTCT GCCAGAGATG AAGTGCAGCA GAAGTGGATT 
AATCAGGATG GCTGTCAGGT TATCTGTGCT ACAATTGCAT TTGGAATGGG GATTGACAAA 
CCGGACGTGC GATTTGTGAT TCATGCATCT CTCCCTAAAT CTGTGGAGGG TTACTACCAA 
GAATCTGGCA GAGCTGGAAG AGATGGGGAA ATATCTCACT GCCTGCTTTT CTATACCTAT 
CATGATGTGA CCAGACTGAA AAGACTTATA ATGATGGAAA AAGATGGAAA CCATCATACA 
AGAGAAACTC ACTTCAATAA TTTGTATAGC ATGGTACATT ACTGTGAAAA TATAACGGAA 
TGCAGGAGAA TACAGCTTTT GGCCTACTTT GGTGAAAATG GATTTAATCC TGATTTTTGT 
AAGAAACACC CAGATGTTTC TTGTGATAAT TGCTGTAAAA CAAAGGATTA TAAAACAAGA 
GATGTGACTG ACGATGTGAA AAGTATTGTA AGATTTGTTC AAGAACATAG TTCATCACAA 
GGAATGAGAA ATATAAAACA TGTAGGTCCT TCTGGAAGAT TTACTATGAA TATGCTGGTC 
GACATTTTCT TGGGGAGTAA GAGTGCAAAA ATCCAGTCAG GTATATTTGG AAAAGGATCT 
GCTTATTCAC GACACAATGC CGAAAGACTT TTTAAAAAGC TGATACTTGA CAAGATTTTG 
GATGAAGACT TATATATCAA TGCCAATGAC CAGGCGATCG CTTATGTGAT GCTCGGAAAT 
AAAGCCCAAA CTGTACTAAA TGGCAATTTA AAGGTAGACT TTATGGAAAC AGAAAATTCC 
AGCAGTGTGA AAAAACAAAA AGCGTTAGTA GCAAAAGTGT CTCAGAGGGA AGAGATGGTT 
AAAAAATGTC TTGGAGAACT TACAGAAGTC TGCAAATCTC TGGGGAAAGT TTTTGGTGTC 
CATTACTTCA ATATTTTTAA TACCGTCACT CTCAAGAAGC TTGCAGAATC TTTATCTTCT 
GATCCTGAGG TTTTGCTTCA AATTGATGGT GTTACTGAAG ACAAACTGGA AAAATATGGT 
GCGGAAGTGA TTTCAGTATT ACAGAAATAC TCTGAATGGA CATCGCCAGC TGAAGACAGT 
TCCCCAGGGA TAAGCCTGTC CAGCAGCAGA GGCCCCGGAA GAAGTGCCGC TGAGGAGCTC 
GACGAGGAAA TACCCGTATC TTCCCACTAC TTTGCAAGTA AAACCAGAAA TGAAAGGAAG 
AGGAAAAAGA TGCCAGCCTC CCAAAGGTCT AAGAGGAGAA AAACTGCTTC CAGTGGTTCC 
AAGGCAAAGG GGGGGTCTGC CACATGTAGA AAGATATCTT CCAAAACGAA ATCCTCCAGC 
ATCATTGGAT CCAGTTCAGC CTCACATACT TCTCAAGCGA CATCAGGAGC CAATAGCAAA 
TTGGGGATTA TGGCTCCACC GAAGCCTATA AATAGACCGT TTCTTAAGCC TTCATATGCA 
TTCTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( NM_001287247.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274176.1 Homo sapiens BLM RecQ like helicase (BLM), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu20391
Clone ID Related Accession (Same CDS sequence)	NM_001287247.1 , NM_001287247.2
Accession Version	NM_001287247.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	Bloom syndrome protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, BC115028.1, BC101567.1, DB520927.1 and AC124248.6. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC115028.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT 
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA 
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA 
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT 
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT 
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT 
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT 
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA 
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC 
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG 
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT 
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT 
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA 
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGAA 
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACTGA AGACAAACTG 
GAAAAATATG GTGCGGAAGT GATTTCAGTA TTACAGAAAT ACTCTGAATG GACATCGCCA 
GCTGAAGACA GTTCCCCAGG GATAAGCCTG TCCAGCAGCA GAGGCCCCGG AAGAAGTGCC 
GCTGAGGAGC TCGACGAGGA AATACCCGTA TCTTCCCACT ACTTTGCAAG TAAAACCAGA 
AATGAAAGGA AGAGGAAAAA GATGCCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTGCT 
TCCAGTGGTT CCAAGGCAAA GGGGGGGTCT GCCACATGTA GAAAGATATC TTCCAAAACG 
AAATCCTCCA GCATCATTGG ATCCAGTTCA GCCTCACATA CTTCTCAAGC GACATCAGGA 
GCCAATAGCA AATTGGGGAT TATGGCTCCA CCGAAGCCTA TAAATAGACC GTTTCTTAAG 
CCTTCATATG CATTCTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274176.1
CDS	119..3979
Translation	MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	NM_001287247.1
Organism	Homo sapiens(human)
Definition	Homo sapiens BLM RecQ like helicase (BLM), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287247.1

ORF Insert Sequence:

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT 
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA 
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA 
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT 
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT 
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT 
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT 
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA 
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC 
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG 
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT 
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT 
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA 
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGAA 
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACTGA AGACAAACTG 
GAAAAATATG GTGCGGAAGT GATTTCAGTA TTACAGAAAT ACTCTGAATG GACATCGCCA 
GCTGAAGACA GTTCCCCAGG GATAAGCCTG TCCAGCAGCA GAGGCCCCGG AAGAAGTGCC 
GCTGAGGAGC TCGACGAGGA AATACCCGTA TCTTCCCACT ACTTTGCAAG TAAAACCAGA 
AATGAAAGGA AGAGGAAAAA GATGCCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTGCT 
TCCAGTGGTT CCAAGGCAAA GGGGGGGTCT GCCACATGTA GAAAGATATC TTCCAAAACG 
AAATCCTCCA GCATCATTGG ATCCAGTTCA GCCTCACATA CTTCTCAAGC GACATCAGGA 
GCCAATAGCA AATTGGGGAT TATGGCTCCA CCGAAGCCTA TAAATAGACC GTTTCTTAAG 
CCTTCATATG CATTCTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BLM ( NM_001287247.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274176.1 Homo sapiens BLM RecQ like helicase (BLM), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu20391
Clone ID Related Accession (Same CDS sequence)	NM_001287247.1 , NM_001287247.2
Accession Version	NM_001287247.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	Bloom syndrome protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, BC115028.1, BC101567.1, DB520927.1 and AC124248.6. On May 31, 2019 this sequence version replaced NM_001287247.1. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC115028.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT 
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA 
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA 
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT 
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT 
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT 
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT 
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA 
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC 
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG 
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT 
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT 
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA 
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGAA 
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACTGA AGACAAACTG 
GAAAAATATG GTGCGGAAGT GATTTCAGTA TTACAGAAAT ACTCTGAATG GACATCGCCA 
GCTGAAGACA GTTCCCCAGG GATAAGCCTG TCCAGCAGCA GAGGCCCCGG AAGAAGTGCC 
GCTGAGGAGC TCGACGAGGA AATACCCGTA TCTTCCCACT ACTTTGCAAG TAAAACCAGA 
AATGAAAGGA AGAGGAAAAA GATGCCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTGCT 
TCCAGTGGTT CCAAGGCAAA GGGGGGGTCT GCCACATGTA GAAAGATATC TTCCAAAACG 
AAATCCTCCA GCATCATTGG ATCCAGTTCA GCCTCACATA CTTCTCAAGC GACATCAGGA 
GCCAATAGCA AATTGGGGAT TATGGCTCCA CCGAAGCCTA TAAATAGACC GTTTCTTAAG 
CCTTCATATG CATTCTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274176.1
CDS	100..3960
Translation	MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS

Target ORF information:

RefSeq Version	NM_001287247.2
Organism	Homo sapiens(human)
Definition	Homo sapiens BLM RecQ like helicase (BLM), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287247.2

ORF Insert Sequence:

ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA 
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA 
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC 
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG 
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC 
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA 
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT 
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA 
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA 
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG 
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT 
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT 
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA 
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT 
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT 
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT 
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC 
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT 
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG 
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT 
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG 
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA 
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT 
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT 
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC 
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA 
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC 
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC 
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT 
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA 
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA 
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT 
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG 
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG 
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC 
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT 
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA 
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG 
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC 
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG 
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC 
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT 
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA 
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA 
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT 
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT 
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT 
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT 
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA 
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC 
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG 
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT 
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT 
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA 
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGAA 
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACTGA AGACAAACTG 
GAAAAATATG GTGCGGAAGT GATTTCAGTA TTACAGAAAT ACTCTGAATG GACATCGCCA 
GCTGAAGACA GTTCCCCAGG GATAAGCCTG TCCAGCAGCA GAGGCCCCGG AAGAAGTGCC 
GCTGAGGAGC TCGACGAGGA AATACCCGTA TCTTCCCACT ACTTTGCAAG TAAAACCAGA 
AATGAAAGGA AGAGGAAAAA GATGCCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTGCT 
TCCAGTGGTT CCAAGGCAAA GGGGGGGTCT GCCACATGTA GAAAGATATC TTCCAAAACG 
AAATCCTCCA GCATCATTGG ATCCAGTTCA GCCTCACATA CTTCTCAAGC GACATCAGGA 
GCCAATAGCA AATTGGGGAT TATGGCTCCA CCGAAGCCTA TAAATAGACC GTTTCTTAAG 
CCTTCATATG CATTCTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	BLM
Entrez Gene ID	641
Full Name	Bloom syndrome RecQ like helicase
Synonyms	BS,RECQ2,RECQL2,RECQL3
General protein information	Preferred Names Bloom syndrome RecQ like helicase Names Bloom syndrome protein Bloom syndrome, RecQ helicase-like DNA helicase, RecQ-like type 2 recQ protein-like 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q26.1
Summary	The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008].
Disorder MIM:	604610
Disorder Html:	Bloom syndrome, 210900 (3)

Pan troglodytes (chimpanzee)	BLM	XP_510594.2
Canis lupus familiaris (dog)	BLM	XP_003434427.1
Mus musculus (house mouse)	Blm	NP_031576.4
	BLM	XP_001097543.1
Gallus gallus (chicken)	BLM	NP_001007088.2
Xenopus tropicalis (tropical clawed frog)	blm	NP_001243837.1
Homo sapiens (human)	BLM	NP_000048.1
Bos taurus (cattle)	BLM	XP_002696629.1
Rattus norvegicus (Norway rat)	Blm	XP_006229520.1
Danio rerio (zebrafish)	blm	XP_701357.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

BLM cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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