huwe1 cDNA ORF clone, Danio rerio(zebrafish)

The following huwe1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the huwe1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa27855 NM_001365654.1
Latest version!
Danio rerio HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa43094 NM_001365655.1
Latest version!
Danio rerio HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_005162278.4
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_009296962.2
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_021473705.1
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_021473704.1
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_021473706.1
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_005162279.3
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27856 XM_021473707.1
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27856 XM_005162280.4
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27857 XM_021473708.1
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27857 XM_005162281.4
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27858 XM_021473709.1
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27858 XM_001923865.6
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27859 XM_021473710.1
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27859 XM_005162282.4
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa43094 XM_009296963.3
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa43094 XM_021473711.1
Latest version!
Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa27855 XM_005162279.2 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27858 XM_001923865.5 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa43094 XM_009296963.2 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27856 XM_005162280.3 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_005162278.3 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_009296962.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27857 XM_005162281.3 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27859 XM_005162282.3 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_009296962.1 , XM_021473704.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version NM_001365654.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-07
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX664745.13. On or before Sep 12, 2018 this sequence version replaced XM_021473705.1, XM_005162279.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01013033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

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    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352583.1
    CDS207..13916
    Translation

    Target ORF information:

    RefSeq Version NM_001365654.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43094
    Clone ID Related Accession (Same CDS sequence) XM_009296963.2 , XM_009296963.3 , XM_021473711.1 , NM_001365655.1
    Accession Version NM_001365655.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-07
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX664745.13. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01013032.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476746, SAMEA4476747 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
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    8401
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    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352584.1
    CDS244..13779
    Translation

    Target ORF information:

    RefSeq Version NM_001365655.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365655.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_009296962.1 , XM_021473704.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_021473705.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
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    2941
    3001
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    3181
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    5881
    5941
    6001
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    7141
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    9301
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    9781
    9841
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    10021
    10081
    10141
    10201
    10261
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    10501
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    11461
    11521
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    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329380.1
    CDS214..13923
    Translation

    Target ORF information:

    RefSeq Version XM_021473705.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473705.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_009296962.1 , XM_021473704.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_005162278.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162278.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162335.1
    CDS149..13858
    Translation

    Target ORF information:

    RefSeq Version XM_005162278.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162278.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_009296962.1 , XM_021473704.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_005162279.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162279.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162336.1
    CDS214..13923
    Translation

    Target ORF information:

    RefSeq Version XM_005162279.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162279.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_009296962.1 , XM_021473704.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_005162279.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005162279.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
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    1801
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    1921
    1981
    2041
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    2161
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    2641
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    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162336.1
    CDS221..13930
    Translation

    Target ORF information:

    RefSeq Version XM_005162279.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162279.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_009296962.1 , XM_021473704.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_021473706.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
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    2641
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    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329381.1
    CDS239..13948
    Translation

    Target ORF information:

    RefSeq Version XM_021473706.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473706.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_009296962.1 , XM_021473704.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_009296962.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1261
    1321
    1381
    1441
    1501
    1561
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    1981
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    12481
    12541
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    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295237.1
    CDS258..13967
    Translation

    Target ORF information:

    RefSeq Version XM_009296962.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296962.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_009296962.1 , XM_021473704.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_021473704.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
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    4141
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    5881
    5941
    6001
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    6301
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    7141
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    9301
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    9781
    9841
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    10021
    10081
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    10201
    10261
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    10501
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    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329379.1
    CDS142..13851
    Translation

    Target ORF information:

    RefSeq Version XM_021473704.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473704.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_009296962.1 , XM_021473704.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_005162278.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162278.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162335.1
    CDS142..13851
    Translation

    Target ORF information:

    RefSeq Version XM_005162278.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162278.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_009296962.1 , XM_021473704.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_009296962.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296962.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295237.1
    CDS239..13948
    Translation

    Target ORF information:

    RefSeq Version XM_009296962.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296962.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27856
    Clone ID Related Accession (Same CDS sequence) XM_021473707.1 , XM_005162280.3 , XM_005162280.4
    Accession Version XM_021473707.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1321
    1381
    1441
    1501
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    1981
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    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGAGAGAGG AGAGGAGCGG GCAAACGCTG TACCAGGGAA TCAAGACCTG
    TGTCAGATCC TGCAGGATGT CGGTGATGAT GTCTACCAGC AGTACAGACT CACCAGACAA
    GGCAGTGATT TTGACTCGCA ATCTGCCTTC CCCATCAATA CTCAGGTGTT TGCTGCAGAT
    GGTGCTGTAG CTGAATCTTC TCAGTCTGGA ACTCCACAGG GAGAAGCCTC TACACCCGAG
    GAAATGCGTG AAGAGAAAAA GGAGCAGGAA GGAGACAAGG GAGCTTCTTC GGAGGAGAGC
    CGAGCTGCTA AGGCTAAAGC TAGCAAACCT CTGATGCCCA CCTCTACCAT CTTGAGGCTG
    TTGGCCGAGC TGGTTCGCTC TTATGTTGGC ATTGCCACTC TTATCACCTC TTACAACTAC
    ACAGCTGGCC AGTCGGAGCT CATTAAGGAA GATTGCAGTG TGTTGGCATT TGTTTTGGAC
    CACTTGCTCC CACACACCCA GAACTCTGAA GATAAGGACA CCCCTGCCCT TGCACGCCTT
    TTCTTGGCCA GCCTTGCTGC AGCTGGCACG GGCACCGATG CCCAGGCTGC ACTGGTCAAC
    GAGGTCAAAG CTGCTTTGAG TCGAGCATTG GGCATGGCAG AGGGCACAGA GAAACACGCC
    CGTTTGCAAG CAGTGATGTG CATCATCAGC ACCATTATGG AGTCGTGTCC TTCCACCTCC
    AGCTTCTACA GCACAGCCAC AGCTAAGACC CAGCACAACG GCATGAACAA TATCATCCGG
    CTCTTCTTGA AGAAAGGCCT TGTCAATGAC CTGGCCCGTG TGCCTCACAG CCTTGATCTA
    TCCAGTCCCA ATATGGCAAA CACAGTAAAT GCTGCTTTGA AGCCTCTGGA AACATTGTCG
    CGAATTGTGA ACCAGCCCAG CAGCCTGTTT GGTGGTAAAG GGGGATCCAG CAAGAACAAG
    ACTGAACATG ACACTGTTGG CACAGTCAGA GACAGTAACA GCAACACTCA GGATCAAGGA
    GAAGTGGGTG AGGCAGAGCC GGTTGAGAAT CGTGACCGAG GTCAAGCCAC AGACTCTGAC
    CTGATGGATG GAGAAGCCGA GGGAGACACT GTGGTCATTG CTGGACAGCC TGAGGTGCTG
    AGCACTCAGG CAATGCAGGT GGAGAACGAG TTGGTGGATT TGATCGATGA ACTTTTAGAG
    AGAGATGCCC AAACTGTTAA TAGTACCATC ATCGTAGGTC GTTCTGGAGA GGATGAGTCT
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329382.1
    CDS142..13848
    Translation

    Target ORF information:

    RefSeq Version XM_021473707.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473707.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGAGAGAGG AGAGGAGCGG GCAAACGCTG TACCAGGGAA TCAAGACCTG
    TGTCAGATCC TGCAGGATGT CGGTGATGAT GTCTACCAGC AGTACAGACT CACCAGACAA
    GGCAGTGATT TTGACTCGCA ATCTGCCTTC CCCATCAATA CTCAGGTGTT TGCTGCAGAT
    GGTGCTGTAG CTGAATCTTC TCAGTCTGGA ACTCCACAGG GAGAAGCCTC TACACCCGAG
    GAAATGCGTG AAGAGAAAAA GGAGCAGGAA GGAGACAAGG GAGCTTCTTC GGAGGAGAGC
    CGAGCTGCTA AGGCTAAAGC TAGCAAACCT CTGATGCCCA CCTCTACCAT CTTGAGGCTG
    TTGGCCGAGC TGGTTCGCTC TTATGTTGGC ATTGCCACTC TTATCACCTC TTACAACTAC
    ACAGCTGGCC AGTCGGAGCT CATTAAGGAA GATTGCAGTG TGTTGGCATT TGTTTTGGAC
    CACTTGCTCC CACACACCCA GAACTCTGAA GATAAGGACA CCCCTGCCCT TGCACGCCTT
    TTCTTGGCCA GCCTTGCTGC AGCTGGCACG GGCACCGATG CCCAGGCTGC ACTGGTCAAC
    GAGGTCAAAG CTGCTTTGAG TCGAGCATTG GGCATGGCAG AGGGCACAGA GAAACACGCC
    CGTTTGCAAG CAGTGATGTG CATCATCAGC ACCATTATGG AGTCGTGTCC TTCCACCTCC
    AGCTTCTACA GCACAGCCAC AGCTAAGACC CAGCACAACG GCATGAACAA TATCATCCGG
    CTCTTCTTGA AGAAAGGCCT TGTCAATGAC CTGGCCCGTG TGCCTCACAG CCTTGATCTA
    TCCAGTCCCA ATATGGCAAA CACAGTAAAT GCTGCTTTGA AGCCTCTGGA AACATTGTCG
    CGAATTGTGA ACCAGCCCAG CAGCCTGTTT GGTGGTAAAG GGGGATCCAG CAAGAACAAG
    ACTGAACATG ACACTGTTGG CACAGTCAGA GACAGTAACA GCAACACTCA GGATCAAGGA
    GAAGTGGGTG AGGCAGAGCC GGTTGAGAAT CGTGACCGAG GTCAAGCCAC AGACTCTGAC
    CTGATGGATG GAGAAGCCGA GGGAGACACT GTGGTCATTG CTGGACAGCC TGAGGTGCTG
    AGCACTCAGG CAATGCAGGT GGAGAACGAG TTGGTGGATT TGATCGATGA ACTTTTAGAG
    AGAGATGCCC AAACTGTTAA TAGTACCATC ATCGTAGGTC GTTCTGGAGA GGATGAGTCT
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27856
    Clone ID Related Accession (Same CDS sequence) XM_021473707.1 , XM_005162280.3 , XM_005162280.4
    Accession Version XM_005162280.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162280.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
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    9781
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    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGAGAGAGG AGAGGAGCGG GCAAACGCTG TACCAGGGAA TCAAGACCTG
    TGTCAGATCC TGCAGGATGT CGGTGATGAT GTCTACCAGC AGTACAGACT CACCAGACAA
    GGCAGTGATT TTGACTCGCA ATCTGCCTTC CCCATCAATA CTCAGGTGTT TGCTGCAGAT
    GGTGCTGTAG CTGAATCTTC TCAGTCTGGA ACTCCACAGG GAGAAGCCTC TACACCCGAG
    GAAATGCGTG AAGAGAAAAA GGAGCAGGAA GGAGACAAGG GAGCTTCTTC GGAGGAGAGC
    CGAGCTGCTA AGGCTAAAGC TAGCAAACCT CTGATGCCCA CCTCTACCAT CTTGAGGCTG
    TTGGCCGAGC TGGTTCGCTC TTATGTTGGC ATTGCCACTC TTATCACCTC TTACAACTAC
    ACAGCTGGCC AGTCGGAGCT CATTAAGGAA GATTGCAGTG TGTTGGCATT TGTTTTGGAC
    CACTTGCTCC CACACACCCA GAACTCTGAA GATAAGGACA CCCCTGCCCT TGCACGCCTT
    TTCTTGGCCA GCCTTGCTGC AGCTGGCACG GGCACCGATG CCCAGGCTGC ACTGGTCAAC
    GAGGTCAAAG CTGCTTTGAG TCGAGCATTG GGCATGGCAG AGGGCACAGA GAAACACGCC
    CGTTTGCAAG CAGTGATGTG CATCATCAGC ACCATTATGG AGTCGTGTCC TTCCACCTCC
    AGCTTCTACA GCACAGCCAC AGCTAAGACC CAGCACAACG GCATGAACAA TATCATCCGG
    CTCTTCTTGA AGAAAGGCCT TGTCAATGAC CTGGCCCGTG TGCCTCACAG CCTTGATCTA
    TCCAGTCCCA ATATGGCAAA CACAGTAAAT GCTGCTTTGA AGCCTCTGGA AACATTGTCG
    CGAATTGTGA ACCAGCCCAG CAGCCTGTTT GGTGGTAAAG GGGGATCCAG CAAGAACAAG
    ACTGAACATG ACACTGTTGG CACAGTCAGA GACAGTAACA GCAACACTCA GGATCAAGGA
    GAAGTGGGTG AGGCAGAGCC GGTTGAGAAT CGTGACCGAG GTCAAGCCAC AGACTCTGAC
    CTGATGGATG GAGAAGCCGA GGGAGACACT GTGGTCATTG CTGGACAGCC TGAGGTGCTG
    AGCACTCAGG CAATGCAGGT GGAGAACGAG TTGGTGGATT TGATCGATGA ACTTTTAGAG
    AGAGATGCCC AAACTGTTAA TAGTACCATC ATCGTAGGTC GTTCTGGAGA GGATGAGTCT
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162337.1
    CDS149..13855
    Translation

    Target ORF information:

    RefSeq Version XM_005162280.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162280.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGAGAGAGG AGAGGAGCGG GCAAACGCTG TACCAGGGAA TCAAGACCTG
    TGTCAGATCC TGCAGGATGT CGGTGATGAT GTCTACCAGC AGTACAGACT CACCAGACAA
    GGCAGTGATT TTGACTCGCA ATCTGCCTTC CCCATCAATA CTCAGGTGTT TGCTGCAGAT
    GGTGCTGTAG CTGAATCTTC TCAGTCTGGA ACTCCACAGG GAGAAGCCTC TACACCCGAG
    GAAATGCGTG AAGAGAAAAA GGAGCAGGAA GGAGACAAGG GAGCTTCTTC GGAGGAGAGC
    CGAGCTGCTA AGGCTAAAGC TAGCAAACCT CTGATGCCCA CCTCTACCAT CTTGAGGCTG
    TTGGCCGAGC TGGTTCGCTC TTATGTTGGC ATTGCCACTC TTATCACCTC TTACAACTAC
    ACAGCTGGCC AGTCGGAGCT CATTAAGGAA GATTGCAGTG TGTTGGCATT TGTTTTGGAC
    CACTTGCTCC CACACACCCA GAACTCTGAA GATAAGGACA CCCCTGCCCT TGCACGCCTT
    TTCTTGGCCA GCCTTGCTGC AGCTGGCACG GGCACCGATG CCCAGGCTGC ACTGGTCAAC
    GAGGTCAAAG CTGCTTTGAG TCGAGCATTG GGCATGGCAG AGGGCACAGA GAAACACGCC
    CGTTTGCAAG CAGTGATGTG CATCATCAGC ACCATTATGG AGTCGTGTCC TTCCACCTCC
    AGCTTCTACA GCACAGCCAC AGCTAAGACC CAGCACAACG GCATGAACAA TATCATCCGG
    CTCTTCTTGA AGAAAGGCCT TGTCAATGAC CTGGCCCGTG TGCCTCACAG CCTTGATCTA
    TCCAGTCCCA ATATGGCAAA CACAGTAAAT GCTGCTTTGA AGCCTCTGGA AACATTGTCG
    CGAATTGTGA ACCAGCCCAG CAGCCTGTTT GGTGGTAAAG GGGGATCCAG CAAGAACAAG
    ACTGAACATG ACACTGTTGG CACAGTCAGA GACAGTAACA GCAACACTCA GGATCAAGGA
    GAAGTGGGTG AGGCAGAGCC GGTTGAGAAT CGTGACCGAG GTCAAGCCAC AGACTCTGAC
    CTGATGGATG GAGAAGCCGA GGGAGACACT GTGGTCATTG CTGGACAGCC TGAGGTGCTG
    AGCACTCAGG CAATGCAGGT GGAGAACGAG TTGGTGGATT TGATCGATGA ACTTTTAGAG
    AGAGATGCCC AAACTGTTAA TAGTACCATC ATCGTAGGTC GTTCTGGAGA GGATGAGTCT
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27856
    Clone ID Related Accession (Same CDS sequence) XM_021473707.1 , XM_005162280.3 , XM_005162280.4
    Accession Version XM_005162280.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162280.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1801
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    1981
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    2641
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    12481
    12541
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    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGAGAGAGG AGAGGAGCGG GCAAACGCTG TACCAGGGAA TCAAGACCTG
    TGTCAGATCC TGCAGGATGT CGGTGATGAT GTCTACCAGC AGTACAGACT CACCAGACAA
    GGCAGTGATT TTGACTCGCA ATCTGCCTTC CCCATCAATA CTCAGGTGTT TGCTGCAGAT
    GGTGCTGTAG CTGAATCTTC TCAGTCTGGA ACTCCACAGG GAGAAGCCTC TACACCCGAG
    GAAATGCGTG AAGAGAAAAA GGAGCAGGAA GGAGACAAGG GAGCTTCTTC GGAGGAGAGC
    CGAGCTGCTA AGGCTAAAGC TAGCAAACCT CTGATGCCCA CCTCTACCAT CTTGAGGCTG
    TTGGCCGAGC TGGTTCGCTC TTATGTTGGC ATTGCCACTC TTATCACCTC TTACAACTAC
    ACAGCTGGCC AGTCGGAGCT CATTAAGGAA GATTGCAGTG TGTTGGCATT TGTTTTGGAC
    CACTTGCTCC CACACACCCA GAACTCTGAA GATAAGGACA CCCCTGCCCT TGCACGCCTT
    TTCTTGGCCA GCCTTGCTGC AGCTGGCACG GGCACCGATG CCCAGGCTGC ACTGGTCAAC
    GAGGTCAAAG CTGCTTTGAG TCGAGCATTG GGCATGGCAG AGGGCACAGA GAAACACGCC
    CGTTTGCAAG CAGTGATGTG CATCATCAGC ACCATTATGG AGTCGTGTCC TTCCACCTCC
    AGCTTCTACA GCACAGCCAC AGCTAAGACC CAGCACAACG GCATGAACAA TATCATCCGG
    CTCTTCTTGA AGAAAGGCCT TGTCAATGAC CTGGCCCGTG TGCCTCACAG CCTTGATCTA
    TCCAGTCCCA ATATGGCAAA CACAGTAAAT GCTGCTTTGA AGCCTCTGGA AACATTGTCG
    CGAATTGTGA ACCAGCCCAG CAGCCTGTTT GGTGGTAAAG GGGGATCCAG CAAGAACAAG
    ACTGAACATG ACACTGTTGG CACAGTCAGA GACAGTAACA GCAACACTCA GGATCAAGGA
    GAAGTGGGTG AGGCAGAGCC GGTTGAGAAT CGTGACCGAG GTCAAGCCAC AGACTCTGAC
    CTGATGGATG GAGAAGCCGA GGGAGACACT GTGGTCATTG CTGGACAGCC TGAGGTGCTG
    AGCACTCAGG CAATGCAGGT GGAGAACGAG TTGGTGGATT TGATCGATGA ACTTTTAGAG
    AGAGATGCCC AAACTGTTAA TAGTACCATC ATCGTAGGTC GTTCTGGAGA GGATGAGTCT
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162337.1
    CDS142..13848
    Translation

    Target ORF information:

    RefSeq Version XM_005162280.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162280.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGAGAGAGG AGAGGAGCGG GCAAACGCTG TACCAGGGAA TCAAGACCTG
    TGTCAGATCC TGCAGGATGT CGGTGATGAT GTCTACCAGC AGTACAGACT CACCAGACAA
    GGCAGTGATT TTGACTCGCA ATCTGCCTTC CCCATCAATA CTCAGGTGTT TGCTGCAGAT
    GGTGCTGTAG CTGAATCTTC TCAGTCTGGA ACTCCACAGG GAGAAGCCTC TACACCCGAG
    GAAATGCGTG AAGAGAAAAA GGAGCAGGAA GGAGACAAGG GAGCTTCTTC GGAGGAGAGC
    CGAGCTGCTA AGGCTAAAGC TAGCAAACCT CTGATGCCCA CCTCTACCAT CTTGAGGCTG
    TTGGCCGAGC TGGTTCGCTC TTATGTTGGC ATTGCCACTC TTATCACCTC TTACAACTAC
    ACAGCTGGCC AGTCGGAGCT CATTAAGGAA GATTGCAGTG TGTTGGCATT TGTTTTGGAC
    CACTTGCTCC CACACACCCA GAACTCTGAA GATAAGGACA CCCCTGCCCT TGCACGCCTT
    TTCTTGGCCA GCCTTGCTGC AGCTGGCACG GGCACCGATG CCCAGGCTGC ACTGGTCAAC
    GAGGTCAAAG CTGCTTTGAG TCGAGCATTG GGCATGGCAG AGGGCACAGA GAAACACGCC
    CGTTTGCAAG CAGTGATGTG CATCATCAGC ACCATTATGG AGTCGTGTCC TTCCACCTCC
    AGCTTCTACA GCACAGCCAC AGCTAAGACC CAGCACAACG GCATGAACAA TATCATCCGG
    CTCTTCTTGA AGAAAGGCCT TGTCAATGAC CTGGCCCGTG TGCCTCACAG CCTTGATCTA
    TCCAGTCCCA ATATGGCAAA CACAGTAAAT GCTGCTTTGA AGCCTCTGGA AACATTGTCG
    CGAATTGTGA ACCAGCCCAG CAGCCTGTTT GGTGGTAAAG GGGGATCCAG CAAGAACAAG
    ACTGAACATG ACACTGTTGG CACAGTCAGA GACAGTAACA GCAACACTCA GGATCAAGGA
    GAAGTGGGTG AGGCAGAGCC GGTTGAGAAT CGTGACCGAG GTCAAGCCAC AGACTCTGAC
    CTGATGGATG GAGAAGCCGA GGGAGACACT GTGGTCATTG CTGGACAGCC TGAGGTGCTG
    AGCACTCAGG CAATGCAGGT GGAGAACGAG TTGGTGGATT TGATCGATGA ACTTTTAGAG
    AGAGATGCCC AAACTGTTAA TAGTACCATC ATCGTAGGTC GTTCTGGAGA GGATGAGTCT
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27857
    Clone ID Related Accession (Same CDS sequence) XM_021473708.1 , XM_005162281.3 , XM_005162281.4
    Accession Version XM_021473708.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
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    9781
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    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGGTC GTTCTGGAGA GGATGAGTCT
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329383.1
    CDS142..13848
    Translation

    Target ORF information:

    RefSeq Version XM_021473708.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473708.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGGTC GTTCTGGAGA GGATGAGTCT
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27857
    Clone ID Related Accession (Same CDS sequence) XM_021473708.1 , XM_005162281.3 , XM_005162281.4
    Accession Version XM_005162281.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162281.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGGTC GTTCTGGAGA GGATGAGTCT
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162338.1
    CDS149..13855
    Translation

    Target ORF information:

    RefSeq Version XM_005162281.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162281.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGGTC GTTCTGGAGA GGATGAGTCT
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27857
    Clone ID Related Accession (Same CDS sequence) XM_021473708.1 , XM_005162281.3 , XM_005162281.4
    Accession Version XM_005162281.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162281.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGGTC GTTCTGGAGA GGATGAGTCT
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162338.1
    CDS142..13848
    Translation

    Target ORF information:

    RefSeq Version XM_005162281.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162281.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGGTC GTTCTGGAGA GGATGAGTCT
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27858
    Clone ID Related Accession (Same CDS sequence) XM_021473709.1 , XM_001923865.5 , XM_001923865.6
    Accession Version XM_021473709.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
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    1801
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    1921
    1981
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    2641
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    12181
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    12301
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    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGCTTCAC TCAGTCCAGA TATAGCTGAA AGTTCAGAGC CTGTGCCAGT GGGTGTGTCT
    CAACTCGAGG GTTCGCCCAT GGATACGAGC AGTCCCGCTT CAGGAACGCA GGAAGAGCCG
    GCTGCCAGCT CAGTTCCATC CAATCACATG TCTCAGGAGC TATCAGGAAG TGGTGACAGC
    GGCCTCACAG ACAGGCAGAC AGATGCAGAA ACTGGATCAA CTTCTGTTTC ATCTCCTGGC
    GAGACCATGC CTCGGAGTGA CAGTGCCGAT AGCCAGTCGC AGGCCATCCA GGAGGAGCCC
    CTCCCTTCCA CCAGCAATGA AGAGGAGGAC CCTCTGGCAG GCATCAGTCT ACCTGAAGGC
    GTGGATCCGT CTTTCTTGGC AGCACTGCCT GAAGACATTC GTCGGGAGGT GCTGCAGAAC
    CAACTGGGAA TCAGACCTCC TTCTCGACCT CCTGTCGCCT CCACTCTGAC AACCACTGCT
    AGTGCTGTGC TAGGAGGAGG GCCAGGGGTG ACCGAAGTCA GCCCTGAATT CTTAGCGGCT
    CTCCCACCTG CAATTCAGGA AGAGGTCCTG GCCCAGCAGC GGGCAGAGCA GCAGCGTCGG
    GAGTTATCCC AGCAACCCAC ACAGGGTGAT CAACCTCTGG ACCCAGTCAC ATTCATCCAG
    ACCCTGCCCT CTGAGCTGCG GCGCTCCGTT CTGGAAGACA TGGAGGACAG TGTACTAGCG
    GTCATGCCTC CAGATATTGC AGCAGAGGCT GCAGCACTGC GAAGGGAACA AGAGGCCAGA
    CAGAGACAAC TGATGCACGA GCGCTTGTTC GGTCACAGCT CCTCTTCAGC TCTGTCTGCC
    ATCTTACGCT CTCCCGCCTT CACCAGCCGC CTCGGGGGCA ACCGTGGGGT ACAGTACACC
    CGGCTGGCTG TGCAAAGAGG AGGGACGTTT CAAATGGGAG GGAGTGCCAA TCATAGCAGA
    CCCTCTAGTT CAAACGTTGA CTCTCTCCTG CGCCTGCGGG GGCGTCTCTT GTTGGACCAC
    GAGGCCTTGT CCTGCCTTCT TGTGCTGCTT TTTGTGGACG AGCCCAAACT TAACACCAGC
    CGCCTCCACC GAGTGCTGCG TAACTTGTGC TATCACTCTC AGACGCGTGG CTGGGTCATC
    CGCAGTCTCC TGTCCATCCT GCAGCGCAGC AGTGAGAGCG AGCTGTGCCT GGAGACTGCA
    CGCTTGGAGG AGTCCCGTGG CAAACGCTCA CTAGGCCAGG GCTCATCTAG TGGAGGAAAG
    GGTTGTTCTT CATCCTCCCA GTCGGCTATA GCCTCTTCGT CATCGTCCTC CTCGTCCTCT
    TCTTCTCTGG AGCTGCTGAA CAGGGTGGAG TCACGGAGTT CCAGTCAGCT CTCCTGGCTG
    TCTGTATCAA TGGACGCAGC TCTTGGATGC AGAACCAACA TCTTTCAGAT CCAGCGAGTA
    TCAGGCAGGA AACACGCAGA GAGGCATTCA GCAGGAGGGA GTGTGAGCGC AGGAGGACTC
    ACAGGTGGAG TGTCAGGAAC AGGAATGACG TGTACAGGGG GAGGTGGGTC AACTGTTCAC
    ATCCATCCAC AGGCTGCGCC GGTGGTCTGT CGACATGTAC TGGACACCCT CATCCAATTG
    GCCAAGGTAT TTCCAAGTCA CTTCACTCAA CAACGCTGTA AAGATCCCCT ATCCTCCTCC
    TCCTCTGAGT TGGACACTCG TCTTTGTGCC AGCTCAACAC CGACTATGAG TGCCAGTTGT
    CGGTCAGGTG GCACCAGTCA GAGTAACTCC TCTGCTACCC CAAGTAGTCT CACTTCTATC
    CACAATTCCC TGAGCGCCTG CTCTGGTACG CCACAGTCCC TAGGCATCTC CACCGACTTT
    TGGGATCTCC TGGTCAAGCT GGACAACATG AATGTGAGCC GAAAGGGGAA GGCCTCCATG
    AAGACTGTCC CTCTAGGGGG TGTCGGCGAG ACAGAGGGTG CGCTGTTCAG CCTTGAGGCG
    TCTCCGCTGG GCCAGCTGAT GAATATGCTG TCCCACCCTG TGATCCGCCG TAGTTCCCTC
    CTCACGGAGA AACTGCTACG TCTGCTGTCT CTGATCTCCA TCGCTCTGCC TGACAACAAG
    GCCACGGAGG TTCCAGCCAG CCATCCAGTG CCTCAGACCC CAACTGCCAC GCCATCTGCA
    CCTGCTCCTC AAACCACAGC AGGGTCAGGG ACCATCTCAG CAGTGGCAGC TACAGGCACT
    ACGCAGGGTT CAGTCGGTTC AGTGGGTACA ACGCCATCTC AACCAGCCGC TACAATATCT
    GTGGTTGCAG GCAGCACCAC CGCCTCTAGC ATTATTGCTG GAAAACTGAA GCCAGCAGTA
    AGCATCACGG ACATTGAATC TAAACTGGCC TCTACTGGAC TTACGGAGAA GCAGCTGCAG
    CTCTCTGTGG AGGTTCTGAC ATCTCACTCG TGTTCAGAGG AAGGTCTGGA GGACGCAGCC
    AACATCTTGC TGCAGCTGTC CAGAGGGGAC GCACCGACCC GAGACACTGT GCTTAGACTT
    CTGCTCAGCG GAGCACGTCA CCTTGGATAC ACTCTCTGCA AACAAATCGG CACTTTATTA
    GCTGAGTTGA GGGAGTATAA TCTAGAGCAG CAGAGAAGAG CACGGGCTGA TGCTCAGTCT
    CCTGAAGCTT CTCATGATGA CCCATCCATC ACCACACGCC TCAAGGGCAA GATGACCAGC
    AGGTTTGACG GGTCAGAGAG TGTGGTGATT GTTGCTGCTC AGAAGCGCAC GTTAGGTGGC
    CGGGAGCTTC AGTTGCCCTG CATGAGCTCG CTCACTTCCA AGACCTCAAC GCAGAAGTTT
    TTCTTGCGGG TACTGCAGGT CATCATCCAG CTTAGAGAAG ACACGCGGCG CGCTAACAAG
    AAAGCCAAGC AGACAGGAGG AAGACTAAGT TCAACCAGTC TGGGCTCGGC CAGTAGTATT
    CAGGCTGCGG TCCGTCAGCT GGAGGCTGAA GCGGACGCCA TCATTCAGAT GGTGAGAGAG
    GGACAGAGGG CACGCCGTCT ACAGCAGGCC CCCCCACCCT CTTCATCTGC ATCCTCTTCC
    GCTGCTGCTG CTGCTGCATC CGCTGCTGTG GCTGTGGCTG GATCGTCTGC CCCCAGTACC
    TCTGCAACCA ACAACCCCCC CGCTGGCACG GCCAGCGCAG CCACGTCGGA GGTTCACACT
    GTTGCCGAGC CACCAGCAGC TCAGAGAGAT GATTCGCCCA TGGATGTGGA TCAGCCCTCT
    CCTTTGGAAC AGGACCAGCC AACATTAGGT GAGGAGAGCA GCAGTCAGCA GGAGCAGGAG
    GAGCGTTTGC CTGACCTGCC GCTGTTAAGT GAGCAGCTGC TTTTGGATGA GCTGTGGGAC
    ATGCTTGGAG AATGTCTGAA AGAGCTTGAA GAGTCTCATG ACCAACATGC AGTTCTAGTG
    CTGCAGCCTG CAGTGGAGGC CTTTTTCTTG GTGCATGCAA CAGAGCGGGA AAGCAAACCA
    CCAGTTCGGG ACACTCGGGA GAGCCAGCTG TCACACATCA AGGATGAGCC TCCACCCCTG
    TCACCCGCCC CTCTCACACC CGCTACCCCC TCCTCTCTGG ACCCCTTTTT TTCCAGAGAG
    CCTTCCTCTA TGCACATCTC TTCCAACCTG CCTCCAGATA CACAGAAGTT CCTCCGTTTC
    GCAGAGACCC ACCGCACAGT GCTGAATCAG ATCCTGCGTC AATCCACCAC TCACCTGGCA
    GACGGGCCTT TCGCTGTTTT GGTTGACTAC ATTCGCATCT TGGACTTTGA CGTCAAGCGC
    AAGTATTTCC GTCAGGAGCT GGAGCGTCTG GATGAAGGCT TGAGGAAAGA GGATATGGCC
    GTCCATGTGA GGAGAGATCA CGTCTTTGAA GATTCTTACA GAGAGCTGCA CCGCAAGAGC
    CCAGAAGACA TGAAGAACAG ATTGTATATA GTATTTGAAG GAGAGGAGGG TCAGGATGCA
    GGCGGGCTGT TAAGAGAGTG GTACATGATC ATCTCCAGAG AAATGTTCAA CCCCATGTAT
    GCCCTGTTTC GCACCTCCCC CGGCGATCGG GTCACCTATA CCATCAACCC CTCCTCCCAC
    TGCAACCCCA ACCATCTCAG CTACTTCAAG TTTGTGGGAC GTGTGGTGGC CAAAGCAGTC
    TACGACAACA GGCTATTAGA GTGTTACTTT ACACGCAGCT TCTACAAGCA CATCCTGGGC
    AAGAGCGTTA GGTATACAGA CATGGAAAGC GAAGACTATC CCTTTTTCCA AGGTCTGGTT
    TATTTGTTGG AGAACGATGT GTCGACTCTG GGCTACGAGC TGACATTCAG CACAGAGGTC
    CAAGAGTTTG GCGTGTGTGA AGTGAGAGAT CTGAAGGCCA ACGGTGCCAA CATTCTTGTC
    ACAGAAGAGA ATAAGAAGGA ATATGTGCAT CTGGTCTGCC AGATGAAGAT GACAGGTGCA
    ATCCGTAAAC AGCTGGCTGC CTTCCTGGAG GGCTTTTATG AGATCATCCC CAAGAGACTG
    ATCTCCATCT TCACTGAGCA AGAGCTGGAG CTGCTCATCT CCGGCCTGCC CACCATTGAC
    ATCGACGACC TCAAGGCCAA TACTGAATAC CATAAATATC AGTCCAGTTC TATTCAGATC
    CAGTGGTTCT GGCGTGCTTT ACGATCCTTC GACCAGGCTG ACAGGGCCAA ATTTCTGCAG
    TTTGTTACGG GCACTTCCAA GGTGCCACTG CAGGGCTTTG CTGCACTGGA GGGAATGAAC
    GGCATTCAGA AGTTCCAGAT CCACCGAGAT GACCGCTCCA CTGACCGCCT GCCCTCTGCG
    CACACTTGCT TTAACCAGTT GGACCTCCCA GCGTACGAGA GCTACGAGAA GTTAAGACAC
    ATGCTGCTGC TGGCCATTCA GGAATGTTCT GAAGGCTTCG GACTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329384.1
    CDS142..13812
    Translation

    Target ORF information:

    RefSeq Version XM_021473709.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473709.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGCTTCAC TCAGTCCAGA TATAGCTGAA AGTTCAGAGC CTGTGCCAGT GGGTGTGTCT
    CAACTCGAGG GTTCGCCCAT GGATACGAGC AGTCCCGCTT CAGGAACGCA GGAAGAGCCG
    GCTGCCAGCT CAGTTCCATC CAATCACATG TCTCAGGAGC TATCAGGAAG TGGTGACAGC
    GGCCTCACAG ACAGGCAGAC AGATGCAGAA ACTGGATCAA CTTCTGTTTC ATCTCCTGGC
    GAGACCATGC CTCGGAGTGA CAGTGCCGAT AGCCAGTCGC AGGCCATCCA GGAGGAGCCC
    CTCCCTTCCA CCAGCAATGA AGAGGAGGAC CCTCTGGCAG GCATCAGTCT ACCTGAAGGC
    GTGGATCCGT CTTTCTTGGC AGCACTGCCT GAAGACATTC GTCGGGAGGT GCTGCAGAAC
    CAACTGGGAA TCAGACCTCC TTCTCGACCT CCTGTCGCCT CCACTCTGAC AACCACTGCT
    AGTGCTGTGC TAGGAGGAGG GCCAGGGGTG ACCGAAGTCA GCCCTGAATT CTTAGCGGCT
    CTCCCACCTG CAATTCAGGA AGAGGTCCTG GCCCAGCAGC GGGCAGAGCA GCAGCGTCGG
    GAGTTATCCC AGCAACCCAC ACAGGGTGAT CAACCTCTGG ACCCAGTCAC ATTCATCCAG
    ACCCTGCCCT CTGAGCTGCG GCGCTCCGTT CTGGAAGACA TGGAGGACAG TGTACTAGCG
    GTCATGCCTC CAGATATTGC AGCAGAGGCT GCAGCACTGC GAAGGGAACA AGAGGCCAGA
    CAGAGACAAC TGATGCACGA GCGCTTGTTC GGTCACAGCT CCTCTTCAGC TCTGTCTGCC
    ATCTTACGCT CTCCCGCCTT CACCAGCCGC CTCGGGGGCA ACCGTGGGGT ACAGTACACC
    CGGCTGGCTG TGCAAAGAGG AGGGACGTTT CAAATGGGAG GGAGTGCCAA TCATAGCAGA
    CCCTCTAGTT CAAACGTTGA CTCTCTCCTG CGCCTGCGGG GGCGTCTCTT GTTGGACCAC
    GAGGCCTTGT CCTGCCTTCT TGTGCTGCTT TTTGTGGACG AGCCCAAACT TAACACCAGC
    CGCCTCCACC GAGTGCTGCG TAACTTGTGC TATCACTCTC AGACGCGTGG CTGGGTCATC
    CGCAGTCTCC TGTCCATCCT GCAGCGCAGC AGTGAGAGCG AGCTGTGCCT GGAGACTGCA
    CGCTTGGAGG AGTCCCGTGG CAAACGCTCA CTAGGCCAGG GCTCATCTAG TGGAGGAAAG
    GGTTGTTCTT CATCCTCCCA GTCGGCTATA GCCTCTTCGT CATCGTCCTC CTCGTCCTCT
    TCTTCTCTGG AGCTGCTGAA CAGGGTGGAG TCACGGAGTT CCAGTCAGCT CTCCTGGCTG
    TCTGTATCAA TGGACGCAGC TCTTGGATGC AGAACCAACA TCTTTCAGAT CCAGCGAGTA
    TCAGGCAGGA AACACGCAGA GAGGCATTCA GCAGGAGGGA GTGTGAGCGC AGGAGGACTC
    ACAGGTGGAG TGTCAGGAAC AGGAATGACG TGTACAGGGG GAGGTGGGTC AACTGTTCAC
    ATCCATCCAC AGGCTGCGCC GGTGGTCTGT CGACATGTAC TGGACACCCT CATCCAATTG
    GCCAAGGTAT TTCCAAGTCA CTTCACTCAA CAACGCTGTA AAGATCCCCT ATCCTCCTCC
    TCCTCTGAGT TGGACACTCG TCTTTGTGCC AGCTCAACAC CGACTATGAG TGCCAGTTGT
    CGGTCAGGTG GCACCAGTCA GAGTAACTCC TCTGCTACCC CAAGTAGTCT CACTTCTATC
    CACAATTCCC TGAGCGCCTG CTCTGGTACG CCACAGTCCC TAGGCATCTC CACCGACTTT
    TGGGATCTCC TGGTCAAGCT GGACAACATG AATGTGAGCC GAAAGGGGAA GGCCTCCATG
    AAGACTGTCC CTCTAGGGGG TGTCGGCGAG ACAGAGGGTG CGCTGTTCAG CCTTGAGGCG
    TCTCCGCTGG GCCAGCTGAT GAATATGCTG TCCCACCCTG TGATCCGCCG TAGTTCCCTC
    CTCACGGAGA AACTGCTACG TCTGCTGTCT CTGATCTCCA TCGCTCTGCC TGACAACAAG
    GCCACGGAGG TTCCAGCCAG CCATCCAGTG CCTCAGACCC CAACTGCCAC GCCATCTGCA
    CCTGCTCCTC AAACCACAGC AGGGTCAGGG ACCATCTCAG CAGTGGCAGC TACAGGCACT
    ACGCAGGGTT CAGTCGGTTC AGTGGGTACA ACGCCATCTC AACCAGCCGC TACAATATCT
    GTGGTTGCAG GCAGCACCAC CGCCTCTAGC ATTATTGCTG GAAAACTGAA GCCAGCAGTA
    AGCATCACGG ACATTGAATC TAAACTGGCC TCTACTGGAC TTACGGAGAA GCAGCTGCAG
    CTCTCTGTGG AGGTTCTGAC ATCTCACTCG TGTTCAGAGG AAGGTCTGGA GGACGCAGCC
    AACATCTTGC TGCAGCTGTC CAGAGGGGAC GCACCGACCC GAGACACTGT GCTTAGACTT
    CTGCTCAGCG GAGCACGTCA CCTTGGATAC ACTCTCTGCA AACAAATCGG CACTTTATTA
    GCTGAGTTGA GGGAGTATAA TCTAGAGCAG CAGAGAAGAG CACGGGCTGA TGCTCAGTCT
    CCTGAAGCTT CTCATGATGA CCCATCCATC ACCACACGCC TCAAGGGCAA GATGACCAGC
    AGGTTTGACG GGTCAGAGAG TGTGGTGATT GTTGCTGCTC AGAAGCGCAC GTTAGGTGGC
    CGGGAGCTTC AGTTGCCCTG CATGAGCTCG CTCACTTCCA AGACCTCAAC GCAGAAGTTT
    TTCTTGCGGG TACTGCAGGT CATCATCCAG CTTAGAGAAG ACACGCGGCG CGCTAACAAG
    AAAGCCAAGC AGACAGGAGG AAGACTAAGT TCAACCAGTC TGGGCTCGGC CAGTAGTATT
    CAGGCTGCGG TCCGTCAGCT GGAGGCTGAA GCGGACGCCA TCATTCAGAT GGTGAGAGAG
    GGACAGAGGG CACGCCGTCT ACAGCAGGCC CCCCCACCCT CTTCATCTGC ATCCTCTTCC
    GCTGCTGCTG CTGCTGCATC CGCTGCTGTG GCTGTGGCTG GATCGTCTGC CCCCAGTACC
    TCTGCAACCA ACAACCCCCC CGCTGGCACG GCCAGCGCAG CCACGTCGGA GGTTCACACT
    GTTGCCGAGC CACCAGCAGC TCAGAGAGAT GATTCGCCCA TGGATGTGGA TCAGCCCTCT
    CCTTTGGAAC AGGACCAGCC AACATTAGGT GAGGAGAGCA GCAGTCAGCA GGAGCAGGAG
    GAGCGTTTGC CTGACCTGCC GCTGTTAAGT GAGCAGCTGC TTTTGGATGA GCTGTGGGAC
    ATGCTTGGAG AATGTCTGAA AGAGCTTGAA GAGTCTCATG ACCAACATGC AGTTCTAGTG
    CTGCAGCCTG CAGTGGAGGC CTTTTTCTTG GTGCATGCAA CAGAGCGGGA AAGCAAACCA
    CCAGTTCGGG ACACTCGGGA GAGCCAGCTG TCACACATCA AGGATGAGCC TCCACCCCTG
    TCACCCGCCC CTCTCACACC CGCTACCCCC TCCTCTCTGG ACCCCTTTTT TTCCAGAGAG
    CCTTCCTCTA TGCACATCTC TTCCAACCTG CCTCCAGATA CACAGAAGTT CCTCCGTTTC
    GCAGAGACCC ACCGCACAGT GCTGAATCAG ATCCTGCGTC AATCCACCAC TCACCTGGCA
    GACGGGCCTT TCGCTGTTTT GGTTGACTAC ATTCGCATCT TGGACTTTGA CGTCAAGCGC
    AAGTATTTCC GTCAGGAGCT GGAGCGTCTG GATGAAGGCT TGAGGAAAGA GGATATGGCC
    GTCCATGTGA GGAGAGATCA CGTCTTTGAA GATTCTTACA GAGAGCTGCA CCGCAAGAGC
    CCAGAAGACA TGAAGAACAG ATTGTATATA GTATTTGAAG GAGAGGAGGG TCAGGATGCA
    GGCGGGCTGT TAAGAGAGTG GTACATGATC ATCTCCAGAG AAATGTTCAA CCCCATGTAT
    GCCCTGTTTC GCACCTCCCC CGGCGATCGG GTCACCTATA CCATCAACCC CTCCTCCCAC
    TGCAACCCCA ACCATCTCAG CTACTTCAAG TTTGTGGGAC GTGTGGTGGC CAAAGCAGTC
    TACGACAACA GGCTATTAGA GTGTTACTTT ACACGCAGCT TCTACAAGCA CATCCTGGGC
    AAGAGCGTTA GGTATACAGA CATGGAAAGC GAAGACTATC CCTTTTTCCA AGGTCTGGTT
    TATTTGTTGG AGAACGATGT GTCGACTCTG GGCTACGAGC TGACATTCAG CACAGAGGTC
    CAAGAGTTTG GCGTGTGTGA AGTGAGAGAT CTGAAGGCCA ACGGTGCCAA CATTCTTGTC
    ACAGAAGAGA ATAAGAAGGA ATATGTGCAT CTGGTCTGCC AGATGAAGAT GACAGGTGCA
    ATCCGTAAAC AGCTGGCTGC CTTCCTGGAG GGCTTTTATG AGATCATCCC CAAGAGACTG
    ATCTCCATCT TCACTGAGCA AGAGCTGGAG CTGCTCATCT CCGGCCTGCC CACCATTGAC
    ATCGACGACC TCAAGGCCAA TACTGAATAC CATAAATATC AGTCCAGTTC TATTCAGATC
    CAGTGGTTCT GGCGTGCTTT ACGATCCTTC GACCAGGCTG ACAGGGCCAA ATTTCTGCAG
    TTTGTTACGG GCACTTCCAA GGTGCCACTG CAGGGCTTTG CTGCACTGGA GGGAATGAAC
    GGCATTCAGA AGTTCCAGAT CCACCGAGAT GACCGCTCCA CTGACCGCCT GCCCTCTGCG
    CACACTTGCT TTAACCAGTT GGACCTCCCA GCGTACGAGA GCTACGAGAA GTTAAGACAC
    ATGCTGCTGC TGGCCATTCA GGAATGTTCT GAAGGCTTCG GACTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27858
    Clone ID Related Accession (Same CDS sequence) XM_021473709.1 , XM_001923865.5 , XM_001923865.6
    Accession Version XM_001923865.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001923865.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
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    3121
    3181
    3241
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    3421
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    4081
    4141
    4201
    4261
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    4381
    4441
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    4681
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    5281
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    5521
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    5881
    5941
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    6181
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    6301
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    6421
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    7141
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    7261
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    9301
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    9421
    9481
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    9721
    9781
    9841
    9901
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    10021
    10081
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    10201
    10261
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    10381
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    10501
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    11521
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    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGCTTCAC TCAGTCCAGA TATAGCTGAA AGTTCAGAGC CTGTGCCAGT GGGTGTGTCT
    CAACTCGAGG GTTCGCCCAT GGATACGAGC AGTCCCGCTT CAGGAACGCA GGAAGAGCCG
    GCTGCCAGCT CAGTTCCATC CAATCACATG TCTCAGGAGC TATCAGGAAG TGGTGACAGC
    GGCCTCACAG ACAGGCAGAC AGATGCAGAA ACTGGATCAA CTTCTGTTTC ATCTCCTGGC
    GAGACCATGC CTCGGAGTGA CAGTGCCGAT AGCCAGTCGC AGGCCATCCA GGAGGAGCCC
    CTCCCTTCCA CCAGCAATGA AGAGGAGGAC CCTCTGGCAG GCATCAGTCT ACCTGAAGGC
    GTGGATCCGT CTTTCTTGGC AGCACTGCCT GAAGACATTC GTCGGGAGGT GCTGCAGAAC
    CAACTGGGAA TCAGACCTCC TTCTCGACCT CCTGTCGCCT CCACTCTGAC AACCACTGCT
    AGTGCTGTGC TAGGAGGAGG GCCAGGGGTG ACCGAAGTCA GCCCTGAATT CTTAGCGGCT
    CTCCCACCTG CAATTCAGGA AGAGGTCCTG GCCCAGCAGC GGGCAGAGCA GCAGCGTCGG
    GAGTTATCCC AGCAACCCAC ACAGGGTGAT CAACCTCTGG ACCCAGTCAC ATTCATCCAG
    ACCCTGCCCT CTGAGCTGCG GCGCTCCGTT CTGGAAGACA TGGAGGACAG TGTACTAGCG
    GTCATGCCTC CAGATATTGC AGCAGAGGCT GCAGCACTGC GAAGGGAACA AGAGGCCAGA
    CAGAGACAAC TGATGCACGA GCGCTTGTTC GGTCACAGCT CCTCTTCAGC TCTGTCTGCC
    ATCTTACGCT CTCCCGCCTT CACCAGCCGC CTCGGGGGCA ACCGTGGGGT ACAGTACACC
    CGGCTGGCTG TGCAAAGAGG AGGGACGTTT CAAATGGGAG GGAGTGCCAA TCATAGCAGA
    CCCTCTAGTT CAAACGTTGA CTCTCTCCTG CGCCTGCGGG GGCGTCTCTT GTTGGACCAC
    GAGGCCTTGT CCTGCCTTCT TGTGCTGCTT TTTGTGGACG AGCCCAAACT TAACACCAGC
    CGCCTCCACC GAGTGCTGCG TAACTTGTGC TATCACTCTC AGACGCGTGG CTGGGTCATC
    CGCAGTCTCC TGTCCATCCT GCAGCGCAGC AGTGAGAGCG AGCTGTGCCT GGAGACTGCA
    CGCTTGGAGG AGTCCCGTGG CAAACGCTCA CTAGGCCAGG GCTCATCTAG TGGAGGAAAG
    GGTTGTTCTT CATCCTCCCA GTCGGCTATA GCCTCTTCGT CATCGTCCTC CTCGTCCTCT
    TCTTCTCTGG AGCTGCTGAA CAGGGTGGAG TCACGGAGTT CCAGTCAGCT CTCCTGGCTG
    TCTGTATCAA TGGACGCAGC TCTTGGATGC AGAACCAACA TCTTTCAGAT CCAGCGAGTA
    TCAGGCAGGA AACACGCAGA GAGGCATTCA GCAGGAGGGA GTGTGAGCGC AGGAGGACTC
    ACAGGTGGAG TGTCAGGAAC AGGAATGACG TGTACAGGGG GAGGTGGGTC AACTGTTCAC
    ATCCATCCAC AGGCTGCGCC GGTGGTCTGT CGACATGTAC TGGACACCCT CATCCAATTG
    GCCAAGGTAT TTCCAAGTCA CTTCACTCAA CAACGCTGTA AAGATCCCCT ATCCTCCTCC
    TCCTCTGAGT TGGACACTCG TCTTTGTGCC AGCTCAACAC CGACTATGAG TGCCAGTTGT
    CGGTCAGGTG GCACCAGTCA GAGTAACTCC TCTGCTACCC CAAGTAGTCT CACTTCTATC
    CACAATTCCC TGAGCGCCTG CTCTGGTACG CCACAGTCCC TAGGCATCTC CACCGACTTT
    TGGGATCTCC TGGTCAAGCT GGACAACATG AATGTGAGCC GAAAGGGGAA GGCCTCCATG
    AAGACTGTCC CTCTAGGGGG TGTCGGCGAG ACAGAGGGTG CGCTGTTCAG CCTTGAGGCG
    TCTCCGCTGG GCCAGCTGAT GAATATGCTG TCCCACCCTG TGATCCGCCG TAGTTCCCTC
    CTCACGGAGA AACTGCTACG TCTGCTGTCT CTGATCTCCA TCGCTCTGCC TGACAACAAG
    GCCACGGAGG TTCCAGCCAG CCATCCAGTG CCTCAGACCC CAACTGCCAC GCCATCTGCA
    CCTGCTCCTC AAACCACAGC AGGGTCAGGG ACCATCTCAG CAGTGGCAGC TACAGGCACT
    ACGCAGGGTT CAGTCGGTTC AGTGGGTACA ACGCCATCTC AACCAGCCGC TACAATATCT
    GTGGTTGCAG GCAGCACCAC CGCCTCTAGC ATTATTGCTG GAAAACTGAA GCCAGCAGTA
    AGCATCACGG ACATTGAATC TAAACTGGCC TCTACTGGAC TTACGGAGAA GCAGCTGCAG
    CTCTCTGTGG AGGTTCTGAC ATCTCACTCG TGTTCAGAGG AAGGTCTGGA GGACGCAGCC
    AACATCTTGC TGCAGCTGTC CAGAGGGGAC GCACCGACCC GAGACACTGT GCTTAGACTT
    CTGCTCAGCG GAGCACGTCA CCTTGGATAC ACTCTCTGCA AACAAATCGG CACTTTATTA
    GCTGAGTTGA GGGAGTATAA TCTAGAGCAG CAGAGAAGAG CACGGGCTGA TGCTCAGTCT
    CCTGAAGCTT CTCATGATGA CCCATCCATC ACCACACGCC TCAAGGGCAA GATGACCAGC
    AGGTTTGACG GGTCAGAGAG TGTGGTGATT GTTGCTGCTC AGAAGCGCAC GTTAGGTGGC
    CGGGAGCTTC AGTTGCCCTG CATGAGCTCG CTCACTTCCA AGACCTCAAC GCAGAAGTTT
    TTCTTGCGGG TACTGCAGGT CATCATCCAG CTTAGAGAAG ACACGCGGCG CGCTAACAAG
    AAAGCCAAGC AGACAGGAGG AAGACTAAGT TCAACCAGTC TGGGCTCGGC CAGTAGTATT
    CAGGCTGCGG TCCGTCAGCT GGAGGCTGAA GCGGACGCCA TCATTCAGAT GGTGAGAGAG
    GGACAGAGGG CACGCCGTCT ACAGCAGGCC CCCCCACCCT CTTCATCTGC ATCCTCTTCC
    GCTGCTGCTG CTGCTGCATC CGCTGCTGTG GCTGTGGCTG GATCGTCTGC CCCCAGTACC
    TCTGCAACCA ACAACCCCCC CGCTGGCACG GCCAGCGCAG CCACGTCGGA GGTTCACACT
    GTTGCCGAGC CACCAGCAGC TCAGAGAGAT GATTCGCCCA TGGATGTGGA TCAGCCCTCT
    CCTTTGGAAC AGGACCAGCC AACATTAGGT GAGGAGAGCA GCAGTCAGCA GGAGCAGGAG
    GAGCGTTTGC CTGACCTGCC GCTGTTAAGT GAGCAGCTGC TTTTGGATGA GCTGTGGGAC
    ATGCTTGGAG AATGTCTGAA AGAGCTTGAA GAGTCTCATG ACCAACATGC AGTTCTAGTG
    CTGCAGCCTG CAGTGGAGGC CTTTTTCTTG GTGCATGCAA CAGAGCGGGA AAGCAAACCA
    CCAGTTCGGG ACACTCGGGA GAGCCAGCTG TCACACATCA AGGATGAGCC TCCACCCCTG
    TCACCCGCCC CTCTCACACC CGCTACCCCC TCCTCTCTGG ACCCCTTTTT TTCCAGAGAG
    CCTTCCTCTA TGCACATCTC TTCCAACCTG CCTCCAGATA CACAGAAGTT CCTCCGTTTC
    GCAGAGACCC ACCGCACAGT GCTGAATCAG ATCCTGCGTC AATCCACCAC TCACCTGGCA
    GACGGGCCTT TCGCTGTTTT GGTTGACTAC ATTCGCATCT TGGACTTTGA CGTCAAGCGC
    AAGTATTTCC GTCAGGAGCT GGAGCGTCTG GATGAAGGCT TGAGGAAAGA GGATATGGCC
    GTCCATGTGA GGAGAGATCA CGTCTTTGAA GATTCTTACA GAGAGCTGCA CCGCAAGAGC
    CCAGAAGACA TGAAGAACAG ATTGTATATA GTATTTGAAG GAGAGGAGGG TCAGGATGCA
    GGCGGGCTGT TAAGAGAGTG GTACATGATC ATCTCCAGAG AAATGTTCAA CCCCATGTAT
    GCCCTGTTTC GCACCTCCCC CGGCGATCGG GTCACCTATA CCATCAACCC CTCCTCCCAC
    TGCAACCCCA ACCATCTCAG CTACTTCAAG TTTGTGGGAC GTGTGGTGGC CAAAGCAGTC
    TACGACAACA GGCTATTAGA GTGTTACTTT ACACGCAGCT TCTACAAGCA CATCCTGGGC
    AAGAGCGTTA GGTATACAGA CATGGAAAGC GAAGACTATC CCTTTTTCCA AGGTCTGGTT
    TATTTGTTGG AGAACGATGT GTCGACTCTG GGCTACGAGC TGACATTCAG CACAGAGGTC
    CAAGAGTTTG GCGTGTGTGA AGTGAGAGAT CTGAAGGCCA ACGGTGCCAA CATTCTTGTC
    ACAGAAGAGA ATAAGAAGGA ATATGTGCAT CTGGTCTGCC AGATGAAGAT GACAGGTGCA
    ATCCGTAAAC AGCTGGCTGC CTTCCTGGAG GGCTTTTATG AGATCATCCC CAAGAGACTG
    ATCTCCATCT TCACTGAGCA AGAGCTGGAG CTGCTCATCT CCGGCCTGCC CACCATTGAC
    ATCGACGACC TCAAGGCCAA TACTGAATAC CATAAATATC AGTCCAGTTC TATTCAGATC
    CAGTGGTTCT GGCGTGCTTT ACGATCCTTC GACCAGGCTG ACAGGGCCAA ATTTCTGCAG
    TTTGTTACGG GCACTTCCAA GGTGCCACTG CAGGGCTTTG CTGCACTGGA GGGAATGAAC
    GGCATTCAGA AGTTCCAGAT CCACCGAGAT GACCGCTCCA CTGACCGCCT GCCCTCTGCG
    CACACTTGCT TTAACCAGTT GGACCTCCCA GCGTACGAGA GCTACGAGAA GTTAAGACAC
    ATGCTGCTGC TGGCCATTCA GGAATGTTCT GAAGGCTTCG GACTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001923900.2
    CDS149..13819
    Translation

    Target ORF information:

    RefSeq Version XM_001923865.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001923865.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGCTTCAC TCAGTCCAGA TATAGCTGAA AGTTCAGAGC CTGTGCCAGT GGGTGTGTCT
    CAACTCGAGG GTTCGCCCAT GGATACGAGC AGTCCCGCTT CAGGAACGCA GGAAGAGCCG
    GCTGCCAGCT CAGTTCCATC CAATCACATG TCTCAGGAGC TATCAGGAAG TGGTGACAGC
    GGCCTCACAG ACAGGCAGAC AGATGCAGAA ACTGGATCAA CTTCTGTTTC ATCTCCTGGC
    GAGACCATGC CTCGGAGTGA CAGTGCCGAT AGCCAGTCGC AGGCCATCCA GGAGGAGCCC
    CTCCCTTCCA CCAGCAATGA AGAGGAGGAC CCTCTGGCAG GCATCAGTCT ACCTGAAGGC
    GTGGATCCGT CTTTCTTGGC AGCACTGCCT GAAGACATTC GTCGGGAGGT GCTGCAGAAC
    CAACTGGGAA TCAGACCTCC TTCTCGACCT CCTGTCGCCT CCACTCTGAC AACCACTGCT
    AGTGCTGTGC TAGGAGGAGG GCCAGGGGTG ACCGAAGTCA GCCCTGAATT CTTAGCGGCT
    CTCCCACCTG CAATTCAGGA AGAGGTCCTG GCCCAGCAGC GGGCAGAGCA GCAGCGTCGG
    GAGTTATCCC AGCAACCCAC ACAGGGTGAT CAACCTCTGG ACCCAGTCAC ATTCATCCAG
    ACCCTGCCCT CTGAGCTGCG GCGCTCCGTT CTGGAAGACA TGGAGGACAG TGTACTAGCG
    GTCATGCCTC CAGATATTGC AGCAGAGGCT GCAGCACTGC GAAGGGAACA AGAGGCCAGA
    CAGAGACAAC TGATGCACGA GCGCTTGTTC GGTCACAGCT CCTCTTCAGC TCTGTCTGCC
    ATCTTACGCT CTCCCGCCTT CACCAGCCGC CTCGGGGGCA ACCGTGGGGT ACAGTACACC
    CGGCTGGCTG TGCAAAGAGG AGGGACGTTT CAAATGGGAG GGAGTGCCAA TCATAGCAGA
    CCCTCTAGTT CAAACGTTGA CTCTCTCCTG CGCCTGCGGG GGCGTCTCTT GTTGGACCAC
    GAGGCCTTGT CCTGCCTTCT TGTGCTGCTT TTTGTGGACG AGCCCAAACT TAACACCAGC
    CGCCTCCACC GAGTGCTGCG TAACTTGTGC TATCACTCTC AGACGCGTGG CTGGGTCATC
    CGCAGTCTCC TGTCCATCCT GCAGCGCAGC AGTGAGAGCG AGCTGTGCCT GGAGACTGCA
    CGCTTGGAGG AGTCCCGTGG CAAACGCTCA CTAGGCCAGG GCTCATCTAG TGGAGGAAAG
    GGTTGTTCTT CATCCTCCCA GTCGGCTATA GCCTCTTCGT CATCGTCCTC CTCGTCCTCT
    TCTTCTCTGG AGCTGCTGAA CAGGGTGGAG TCACGGAGTT CCAGTCAGCT CTCCTGGCTG
    TCTGTATCAA TGGACGCAGC TCTTGGATGC AGAACCAACA TCTTTCAGAT CCAGCGAGTA
    TCAGGCAGGA AACACGCAGA GAGGCATTCA GCAGGAGGGA GTGTGAGCGC AGGAGGACTC
    ACAGGTGGAG TGTCAGGAAC AGGAATGACG TGTACAGGGG GAGGTGGGTC AACTGTTCAC
    ATCCATCCAC AGGCTGCGCC GGTGGTCTGT CGACATGTAC TGGACACCCT CATCCAATTG
    GCCAAGGTAT TTCCAAGTCA CTTCACTCAA CAACGCTGTA AAGATCCCCT ATCCTCCTCC
    TCCTCTGAGT TGGACACTCG TCTTTGTGCC AGCTCAACAC CGACTATGAG TGCCAGTTGT
    CGGTCAGGTG GCACCAGTCA GAGTAACTCC TCTGCTACCC CAAGTAGTCT CACTTCTATC
    CACAATTCCC TGAGCGCCTG CTCTGGTACG CCACAGTCCC TAGGCATCTC CACCGACTTT
    TGGGATCTCC TGGTCAAGCT GGACAACATG AATGTGAGCC GAAAGGGGAA GGCCTCCATG
    AAGACTGTCC CTCTAGGGGG TGTCGGCGAG ACAGAGGGTG CGCTGTTCAG CCTTGAGGCG
    TCTCCGCTGG GCCAGCTGAT GAATATGCTG TCCCACCCTG TGATCCGCCG TAGTTCCCTC
    CTCACGGAGA AACTGCTACG TCTGCTGTCT CTGATCTCCA TCGCTCTGCC TGACAACAAG
    GCCACGGAGG TTCCAGCCAG CCATCCAGTG CCTCAGACCC CAACTGCCAC GCCATCTGCA
    CCTGCTCCTC AAACCACAGC AGGGTCAGGG ACCATCTCAG CAGTGGCAGC TACAGGCACT
    ACGCAGGGTT CAGTCGGTTC AGTGGGTACA ACGCCATCTC AACCAGCCGC TACAATATCT
    GTGGTTGCAG GCAGCACCAC CGCCTCTAGC ATTATTGCTG GAAAACTGAA GCCAGCAGTA
    AGCATCACGG ACATTGAATC TAAACTGGCC TCTACTGGAC TTACGGAGAA GCAGCTGCAG
    CTCTCTGTGG AGGTTCTGAC ATCTCACTCG TGTTCAGAGG AAGGTCTGGA GGACGCAGCC
    AACATCTTGC TGCAGCTGTC CAGAGGGGAC GCACCGACCC GAGACACTGT GCTTAGACTT
    CTGCTCAGCG GAGCACGTCA CCTTGGATAC ACTCTCTGCA AACAAATCGG CACTTTATTA
    GCTGAGTTGA GGGAGTATAA TCTAGAGCAG CAGAGAAGAG CACGGGCTGA TGCTCAGTCT
    CCTGAAGCTT CTCATGATGA CCCATCCATC ACCACACGCC TCAAGGGCAA GATGACCAGC
    AGGTTTGACG GGTCAGAGAG TGTGGTGATT GTTGCTGCTC AGAAGCGCAC GTTAGGTGGC
    CGGGAGCTTC AGTTGCCCTG CATGAGCTCG CTCACTTCCA AGACCTCAAC GCAGAAGTTT
    TTCTTGCGGG TACTGCAGGT CATCATCCAG CTTAGAGAAG ACACGCGGCG CGCTAACAAG
    AAAGCCAAGC AGACAGGAGG AAGACTAAGT TCAACCAGTC TGGGCTCGGC CAGTAGTATT
    CAGGCTGCGG TCCGTCAGCT GGAGGCTGAA GCGGACGCCA TCATTCAGAT GGTGAGAGAG
    GGACAGAGGG CACGCCGTCT ACAGCAGGCC CCCCCACCCT CTTCATCTGC ATCCTCTTCC
    GCTGCTGCTG CTGCTGCATC CGCTGCTGTG GCTGTGGCTG GATCGTCTGC CCCCAGTACC
    TCTGCAACCA ACAACCCCCC CGCTGGCACG GCCAGCGCAG CCACGTCGGA GGTTCACACT
    GTTGCCGAGC CACCAGCAGC TCAGAGAGAT GATTCGCCCA TGGATGTGGA TCAGCCCTCT
    CCTTTGGAAC AGGACCAGCC AACATTAGGT GAGGAGAGCA GCAGTCAGCA GGAGCAGGAG
    GAGCGTTTGC CTGACCTGCC GCTGTTAAGT GAGCAGCTGC TTTTGGATGA GCTGTGGGAC
    ATGCTTGGAG AATGTCTGAA AGAGCTTGAA GAGTCTCATG ACCAACATGC AGTTCTAGTG
    CTGCAGCCTG CAGTGGAGGC CTTTTTCTTG GTGCATGCAA CAGAGCGGGA AAGCAAACCA
    CCAGTTCGGG ACACTCGGGA GAGCCAGCTG TCACACATCA AGGATGAGCC TCCACCCCTG
    TCACCCGCCC CTCTCACACC CGCTACCCCC TCCTCTCTGG ACCCCTTTTT TTCCAGAGAG
    CCTTCCTCTA TGCACATCTC TTCCAACCTG CCTCCAGATA CACAGAAGTT CCTCCGTTTC
    GCAGAGACCC ACCGCACAGT GCTGAATCAG ATCCTGCGTC AATCCACCAC TCACCTGGCA
    GACGGGCCTT TCGCTGTTTT GGTTGACTAC ATTCGCATCT TGGACTTTGA CGTCAAGCGC
    AAGTATTTCC GTCAGGAGCT GGAGCGTCTG GATGAAGGCT TGAGGAAAGA GGATATGGCC
    GTCCATGTGA GGAGAGATCA CGTCTTTGAA GATTCTTACA GAGAGCTGCA CCGCAAGAGC
    CCAGAAGACA TGAAGAACAG ATTGTATATA GTATTTGAAG GAGAGGAGGG TCAGGATGCA
    GGCGGGCTGT TAAGAGAGTG GTACATGATC ATCTCCAGAG AAATGTTCAA CCCCATGTAT
    GCCCTGTTTC GCACCTCCCC CGGCGATCGG GTCACCTATA CCATCAACCC CTCCTCCCAC
    TGCAACCCCA ACCATCTCAG CTACTTCAAG TTTGTGGGAC GTGTGGTGGC CAAAGCAGTC
    TACGACAACA GGCTATTAGA GTGTTACTTT ACACGCAGCT TCTACAAGCA CATCCTGGGC
    AAGAGCGTTA GGTATACAGA CATGGAAAGC GAAGACTATC CCTTTTTCCA AGGTCTGGTT
    TATTTGTTGG AGAACGATGT GTCGACTCTG GGCTACGAGC TGACATTCAG CACAGAGGTC
    CAAGAGTTTG GCGTGTGTGA AGTGAGAGAT CTGAAGGCCA ACGGTGCCAA CATTCTTGTC
    ACAGAAGAGA ATAAGAAGGA ATATGTGCAT CTGGTCTGCC AGATGAAGAT GACAGGTGCA
    ATCCGTAAAC AGCTGGCTGC CTTCCTGGAG GGCTTTTATG AGATCATCCC CAAGAGACTG
    ATCTCCATCT TCACTGAGCA AGAGCTGGAG CTGCTCATCT CCGGCCTGCC CACCATTGAC
    ATCGACGACC TCAAGGCCAA TACTGAATAC CATAAATATC AGTCCAGTTC TATTCAGATC
    CAGTGGTTCT GGCGTGCTTT ACGATCCTTC GACCAGGCTG ACAGGGCCAA ATTTCTGCAG
    TTTGTTACGG GCACTTCCAA GGTGCCACTG CAGGGCTTTG CTGCACTGGA GGGAATGAAC
    GGCATTCAGA AGTTCCAGAT CCACCGAGAT GACCGCTCCA CTGACCGCCT GCCCTCTGCG
    CACACTTGCT TTAACCAGTT GGACCTCCCA GCGTACGAGA GCTACGAGAA GTTAAGACAC
    ATGCTGCTGC TGGCCATTCA GGAATGTTCT GAAGGCTTCG GACTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27858
    Clone ID Related Accession (Same CDS sequence) XM_021473709.1 , XM_001923865.5 , XM_001923865.6
    Accession Version XM_001923865.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001923865.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    12781
    12841
    12901
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    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGCTTCAC TCAGTCCAGA TATAGCTGAA AGTTCAGAGC CTGTGCCAGT GGGTGTGTCT
    CAACTCGAGG GTTCGCCCAT GGATACGAGC AGTCCCGCTT CAGGAACGCA GGAAGAGCCG
    GCTGCCAGCT CAGTTCCATC CAATCACATG TCTCAGGAGC TATCAGGAAG TGGTGACAGC
    GGCCTCACAG ACAGGCAGAC AGATGCAGAA ACTGGATCAA CTTCTGTTTC ATCTCCTGGC
    GAGACCATGC CTCGGAGTGA CAGTGCCGAT AGCCAGTCGC AGGCCATCCA GGAGGAGCCC
    CTCCCTTCCA CCAGCAATGA AGAGGAGGAC CCTCTGGCAG GCATCAGTCT ACCTGAAGGC
    GTGGATCCGT CTTTCTTGGC AGCACTGCCT GAAGACATTC GTCGGGAGGT GCTGCAGAAC
    CAACTGGGAA TCAGACCTCC TTCTCGACCT CCTGTCGCCT CCACTCTGAC AACCACTGCT
    AGTGCTGTGC TAGGAGGAGG GCCAGGGGTG ACCGAAGTCA GCCCTGAATT CTTAGCGGCT
    CTCCCACCTG CAATTCAGGA AGAGGTCCTG GCCCAGCAGC GGGCAGAGCA GCAGCGTCGG
    GAGTTATCCC AGCAACCCAC ACAGGGTGAT CAACCTCTGG ACCCAGTCAC ATTCATCCAG
    ACCCTGCCCT CTGAGCTGCG GCGCTCCGTT CTGGAAGACA TGGAGGACAG TGTACTAGCG
    GTCATGCCTC CAGATATTGC AGCAGAGGCT GCAGCACTGC GAAGGGAACA AGAGGCCAGA
    CAGAGACAAC TGATGCACGA GCGCTTGTTC GGTCACAGCT CCTCTTCAGC TCTGTCTGCC
    ATCTTACGCT CTCCCGCCTT CACCAGCCGC CTCGGGGGCA ACCGTGGGGT ACAGTACACC
    CGGCTGGCTG TGCAAAGAGG AGGGACGTTT CAAATGGGAG GGAGTGCCAA TCATAGCAGA
    CCCTCTAGTT CAAACGTTGA CTCTCTCCTG CGCCTGCGGG GGCGTCTCTT GTTGGACCAC
    GAGGCCTTGT CCTGCCTTCT TGTGCTGCTT TTTGTGGACG AGCCCAAACT TAACACCAGC
    CGCCTCCACC GAGTGCTGCG TAACTTGTGC TATCACTCTC AGACGCGTGG CTGGGTCATC
    CGCAGTCTCC TGTCCATCCT GCAGCGCAGC AGTGAGAGCG AGCTGTGCCT GGAGACTGCA
    CGCTTGGAGG AGTCCCGTGG CAAACGCTCA CTAGGCCAGG GCTCATCTAG TGGAGGAAAG
    GGTTGTTCTT CATCCTCCCA GTCGGCTATA GCCTCTTCGT CATCGTCCTC CTCGTCCTCT
    TCTTCTCTGG AGCTGCTGAA CAGGGTGGAG TCACGGAGTT CCAGTCAGCT CTCCTGGCTG
    TCTGTATCAA TGGACGCAGC TCTTGGATGC AGAACCAACA TCTTTCAGAT CCAGCGAGTA
    TCAGGCAGGA AACACGCAGA GAGGCATTCA GCAGGAGGGA GTGTGAGCGC AGGAGGACTC
    ACAGGTGGAG TGTCAGGAAC AGGAATGACG TGTACAGGGG GAGGTGGGTC AACTGTTCAC
    ATCCATCCAC AGGCTGCGCC GGTGGTCTGT CGACATGTAC TGGACACCCT CATCCAATTG
    GCCAAGGTAT TTCCAAGTCA CTTCACTCAA CAACGCTGTA AAGATCCCCT ATCCTCCTCC
    TCCTCTGAGT TGGACACTCG TCTTTGTGCC AGCTCAACAC CGACTATGAG TGCCAGTTGT
    CGGTCAGGTG GCACCAGTCA GAGTAACTCC TCTGCTACCC CAAGTAGTCT CACTTCTATC
    CACAATTCCC TGAGCGCCTG CTCTGGTACG CCACAGTCCC TAGGCATCTC CACCGACTTT
    TGGGATCTCC TGGTCAAGCT GGACAACATG AATGTGAGCC GAAAGGGGAA GGCCTCCATG
    AAGACTGTCC CTCTAGGGGG TGTCGGCGAG ACAGAGGGTG CGCTGTTCAG CCTTGAGGCG
    TCTCCGCTGG GCCAGCTGAT GAATATGCTG TCCCACCCTG TGATCCGCCG TAGTTCCCTC
    CTCACGGAGA AACTGCTACG TCTGCTGTCT CTGATCTCCA TCGCTCTGCC TGACAACAAG
    GCCACGGAGG TTCCAGCCAG CCATCCAGTG CCTCAGACCC CAACTGCCAC GCCATCTGCA
    CCTGCTCCTC AAACCACAGC AGGGTCAGGG ACCATCTCAG CAGTGGCAGC TACAGGCACT
    ACGCAGGGTT CAGTCGGTTC AGTGGGTACA ACGCCATCTC AACCAGCCGC TACAATATCT
    GTGGTTGCAG GCAGCACCAC CGCCTCTAGC ATTATTGCTG GAAAACTGAA GCCAGCAGTA
    AGCATCACGG ACATTGAATC TAAACTGGCC TCTACTGGAC TTACGGAGAA GCAGCTGCAG
    CTCTCTGTGG AGGTTCTGAC ATCTCACTCG TGTTCAGAGG AAGGTCTGGA GGACGCAGCC
    AACATCTTGC TGCAGCTGTC CAGAGGGGAC GCACCGACCC GAGACACTGT GCTTAGACTT
    CTGCTCAGCG GAGCACGTCA CCTTGGATAC ACTCTCTGCA AACAAATCGG CACTTTATTA
    GCTGAGTTGA GGGAGTATAA TCTAGAGCAG CAGAGAAGAG CACGGGCTGA TGCTCAGTCT
    CCTGAAGCTT CTCATGATGA CCCATCCATC ACCACACGCC TCAAGGGCAA GATGACCAGC
    AGGTTTGACG GGTCAGAGAG TGTGGTGATT GTTGCTGCTC AGAAGCGCAC GTTAGGTGGC
    CGGGAGCTTC AGTTGCCCTG CATGAGCTCG CTCACTTCCA AGACCTCAAC GCAGAAGTTT
    TTCTTGCGGG TACTGCAGGT CATCATCCAG CTTAGAGAAG ACACGCGGCG CGCTAACAAG
    AAAGCCAAGC AGACAGGAGG AAGACTAAGT TCAACCAGTC TGGGCTCGGC CAGTAGTATT
    CAGGCTGCGG TCCGTCAGCT GGAGGCTGAA GCGGACGCCA TCATTCAGAT GGTGAGAGAG
    GGACAGAGGG CACGCCGTCT ACAGCAGGCC CCCCCACCCT CTTCATCTGC ATCCTCTTCC
    GCTGCTGCTG CTGCTGCATC CGCTGCTGTG GCTGTGGCTG GATCGTCTGC CCCCAGTACC
    TCTGCAACCA ACAACCCCCC CGCTGGCACG GCCAGCGCAG CCACGTCGGA GGTTCACACT
    GTTGCCGAGC CACCAGCAGC TCAGAGAGAT GATTCGCCCA TGGATGTGGA TCAGCCCTCT
    CCTTTGGAAC AGGACCAGCC AACATTAGGT GAGGAGAGCA GCAGTCAGCA GGAGCAGGAG
    GAGCGTTTGC CTGACCTGCC GCTGTTAAGT GAGCAGCTGC TTTTGGATGA GCTGTGGGAC
    ATGCTTGGAG AATGTCTGAA AGAGCTTGAA GAGTCTCATG ACCAACATGC AGTTCTAGTG
    CTGCAGCCTG CAGTGGAGGC CTTTTTCTTG GTGCATGCAA CAGAGCGGGA AAGCAAACCA
    CCAGTTCGGG ACACTCGGGA GAGCCAGCTG TCACACATCA AGGATGAGCC TCCACCCCTG
    TCACCCGCCC CTCTCACACC CGCTACCCCC TCCTCTCTGG ACCCCTTTTT TTCCAGAGAG
    CCTTCCTCTA TGCACATCTC TTCCAACCTG CCTCCAGATA CACAGAAGTT CCTCCGTTTC
    GCAGAGACCC ACCGCACAGT GCTGAATCAG ATCCTGCGTC AATCCACCAC TCACCTGGCA
    GACGGGCCTT TCGCTGTTTT GGTTGACTAC ATTCGCATCT TGGACTTTGA CGTCAAGCGC
    AAGTATTTCC GTCAGGAGCT GGAGCGTCTG GATGAAGGCT TGAGGAAAGA GGATATGGCC
    GTCCATGTGA GGAGAGATCA CGTCTTTGAA GATTCTTACA GAGAGCTGCA CCGCAAGAGC
    CCAGAAGACA TGAAGAACAG ATTGTATATA GTATTTGAAG GAGAGGAGGG TCAGGATGCA
    GGCGGGCTGT TAAGAGAGTG GTACATGATC ATCTCCAGAG AAATGTTCAA CCCCATGTAT
    GCCCTGTTTC GCACCTCCCC CGGCGATCGG GTCACCTATA CCATCAACCC CTCCTCCCAC
    TGCAACCCCA ACCATCTCAG CTACTTCAAG TTTGTGGGAC GTGTGGTGGC CAAAGCAGTC
    TACGACAACA GGCTATTAGA GTGTTACTTT ACACGCAGCT TCTACAAGCA CATCCTGGGC
    AAGAGCGTTA GGTATACAGA CATGGAAAGC GAAGACTATC CCTTTTTCCA AGGTCTGGTT
    TATTTGTTGG AGAACGATGT GTCGACTCTG GGCTACGAGC TGACATTCAG CACAGAGGTC
    CAAGAGTTTG GCGTGTGTGA AGTGAGAGAT CTGAAGGCCA ACGGTGCCAA CATTCTTGTC
    ACAGAAGAGA ATAAGAAGGA ATATGTGCAT CTGGTCTGCC AGATGAAGAT GACAGGTGCA
    ATCCGTAAAC AGCTGGCTGC CTTCCTGGAG GGCTTTTATG AGATCATCCC CAAGAGACTG
    ATCTCCATCT TCACTGAGCA AGAGCTGGAG CTGCTCATCT CCGGCCTGCC CACCATTGAC
    ATCGACGACC TCAAGGCCAA TACTGAATAC CATAAATATC AGTCCAGTTC TATTCAGATC
    CAGTGGTTCT GGCGTGCTTT ACGATCCTTC GACCAGGCTG ACAGGGCCAA ATTTCTGCAG
    TTTGTTACGG GCACTTCCAA GGTGCCACTG CAGGGCTTTG CTGCACTGGA GGGAATGAAC
    GGCATTCAGA AGTTCCAGAT CCACCGAGAT GACCGCTCCA CTGACCGCCT GCCCTCTGCG
    CACACTTGCT TTAACCAGTT GGACCTCCCA GCGTACGAGA GCTACGAGAA GTTAAGACAC
    ATGCTGCTGC TGGCCATTCA GGAATGTTCT GAAGGCTTCG GACTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001923900.2
    CDS142..13812
    Translation

    Target ORF information:

    RefSeq Version XM_001923865.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001923865.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGCTTCAC TCAGTCCAGA TATAGCTGAA AGTTCAGAGC CTGTGCCAGT GGGTGTGTCT
    CAACTCGAGG GTTCGCCCAT GGATACGAGC AGTCCCGCTT CAGGAACGCA GGAAGAGCCG
    GCTGCCAGCT CAGTTCCATC CAATCACATG TCTCAGGAGC TATCAGGAAG TGGTGACAGC
    GGCCTCACAG ACAGGCAGAC AGATGCAGAA ACTGGATCAA CTTCTGTTTC ATCTCCTGGC
    GAGACCATGC CTCGGAGTGA CAGTGCCGAT AGCCAGTCGC AGGCCATCCA GGAGGAGCCC
    CTCCCTTCCA CCAGCAATGA AGAGGAGGAC CCTCTGGCAG GCATCAGTCT ACCTGAAGGC
    GTGGATCCGT CTTTCTTGGC AGCACTGCCT GAAGACATTC GTCGGGAGGT GCTGCAGAAC
    CAACTGGGAA TCAGACCTCC TTCTCGACCT CCTGTCGCCT CCACTCTGAC AACCACTGCT
    AGTGCTGTGC TAGGAGGAGG GCCAGGGGTG ACCGAAGTCA GCCCTGAATT CTTAGCGGCT
    CTCCCACCTG CAATTCAGGA AGAGGTCCTG GCCCAGCAGC GGGCAGAGCA GCAGCGTCGG
    GAGTTATCCC AGCAACCCAC ACAGGGTGAT CAACCTCTGG ACCCAGTCAC ATTCATCCAG
    ACCCTGCCCT CTGAGCTGCG GCGCTCCGTT CTGGAAGACA TGGAGGACAG TGTACTAGCG
    GTCATGCCTC CAGATATTGC AGCAGAGGCT GCAGCACTGC GAAGGGAACA AGAGGCCAGA
    CAGAGACAAC TGATGCACGA GCGCTTGTTC GGTCACAGCT CCTCTTCAGC TCTGTCTGCC
    ATCTTACGCT CTCCCGCCTT CACCAGCCGC CTCGGGGGCA ACCGTGGGGT ACAGTACACC
    CGGCTGGCTG TGCAAAGAGG AGGGACGTTT CAAATGGGAG GGAGTGCCAA TCATAGCAGA
    CCCTCTAGTT CAAACGTTGA CTCTCTCCTG CGCCTGCGGG GGCGTCTCTT GTTGGACCAC
    GAGGCCTTGT CCTGCCTTCT TGTGCTGCTT TTTGTGGACG AGCCCAAACT TAACACCAGC
    CGCCTCCACC GAGTGCTGCG TAACTTGTGC TATCACTCTC AGACGCGTGG CTGGGTCATC
    CGCAGTCTCC TGTCCATCCT GCAGCGCAGC AGTGAGAGCG AGCTGTGCCT GGAGACTGCA
    CGCTTGGAGG AGTCCCGTGG CAAACGCTCA CTAGGCCAGG GCTCATCTAG TGGAGGAAAG
    GGTTGTTCTT CATCCTCCCA GTCGGCTATA GCCTCTTCGT CATCGTCCTC CTCGTCCTCT
    TCTTCTCTGG AGCTGCTGAA CAGGGTGGAG TCACGGAGTT CCAGTCAGCT CTCCTGGCTG
    TCTGTATCAA TGGACGCAGC TCTTGGATGC AGAACCAACA TCTTTCAGAT CCAGCGAGTA
    TCAGGCAGGA AACACGCAGA GAGGCATTCA GCAGGAGGGA GTGTGAGCGC AGGAGGACTC
    ACAGGTGGAG TGTCAGGAAC AGGAATGACG TGTACAGGGG GAGGTGGGTC AACTGTTCAC
    ATCCATCCAC AGGCTGCGCC GGTGGTCTGT CGACATGTAC TGGACACCCT CATCCAATTG
    GCCAAGGTAT TTCCAAGTCA CTTCACTCAA CAACGCTGTA AAGATCCCCT ATCCTCCTCC
    TCCTCTGAGT TGGACACTCG TCTTTGTGCC AGCTCAACAC CGACTATGAG TGCCAGTTGT
    CGGTCAGGTG GCACCAGTCA GAGTAACTCC TCTGCTACCC CAAGTAGTCT CACTTCTATC
    CACAATTCCC TGAGCGCCTG CTCTGGTACG CCACAGTCCC TAGGCATCTC CACCGACTTT
    TGGGATCTCC TGGTCAAGCT GGACAACATG AATGTGAGCC GAAAGGGGAA GGCCTCCATG
    AAGACTGTCC CTCTAGGGGG TGTCGGCGAG ACAGAGGGTG CGCTGTTCAG CCTTGAGGCG
    TCTCCGCTGG GCCAGCTGAT GAATATGCTG TCCCACCCTG TGATCCGCCG TAGTTCCCTC
    CTCACGGAGA AACTGCTACG TCTGCTGTCT CTGATCTCCA TCGCTCTGCC TGACAACAAG
    GCCACGGAGG TTCCAGCCAG CCATCCAGTG CCTCAGACCC CAACTGCCAC GCCATCTGCA
    CCTGCTCCTC AAACCACAGC AGGGTCAGGG ACCATCTCAG CAGTGGCAGC TACAGGCACT
    ACGCAGGGTT CAGTCGGTTC AGTGGGTACA ACGCCATCTC AACCAGCCGC TACAATATCT
    GTGGTTGCAG GCAGCACCAC CGCCTCTAGC ATTATTGCTG GAAAACTGAA GCCAGCAGTA
    AGCATCACGG ACATTGAATC TAAACTGGCC TCTACTGGAC TTACGGAGAA GCAGCTGCAG
    CTCTCTGTGG AGGTTCTGAC ATCTCACTCG TGTTCAGAGG AAGGTCTGGA GGACGCAGCC
    AACATCTTGC TGCAGCTGTC CAGAGGGGAC GCACCGACCC GAGACACTGT GCTTAGACTT
    CTGCTCAGCG GAGCACGTCA CCTTGGATAC ACTCTCTGCA AACAAATCGG CACTTTATTA
    GCTGAGTTGA GGGAGTATAA TCTAGAGCAG CAGAGAAGAG CACGGGCTGA TGCTCAGTCT
    CCTGAAGCTT CTCATGATGA CCCATCCATC ACCACACGCC TCAAGGGCAA GATGACCAGC
    AGGTTTGACG GGTCAGAGAG TGTGGTGATT GTTGCTGCTC AGAAGCGCAC GTTAGGTGGC
    CGGGAGCTTC AGTTGCCCTG CATGAGCTCG CTCACTTCCA AGACCTCAAC GCAGAAGTTT
    TTCTTGCGGG TACTGCAGGT CATCATCCAG CTTAGAGAAG ACACGCGGCG CGCTAACAAG
    AAAGCCAAGC AGACAGGAGG AAGACTAAGT TCAACCAGTC TGGGCTCGGC CAGTAGTATT
    CAGGCTGCGG TCCGTCAGCT GGAGGCTGAA GCGGACGCCA TCATTCAGAT GGTGAGAGAG
    GGACAGAGGG CACGCCGTCT ACAGCAGGCC CCCCCACCCT CTTCATCTGC ATCCTCTTCC
    GCTGCTGCTG CTGCTGCATC CGCTGCTGTG GCTGTGGCTG GATCGTCTGC CCCCAGTACC
    TCTGCAACCA ACAACCCCCC CGCTGGCACG GCCAGCGCAG CCACGTCGGA GGTTCACACT
    GTTGCCGAGC CACCAGCAGC TCAGAGAGAT GATTCGCCCA TGGATGTGGA TCAGCCCTCT
    CCTTTGGAAC AGGACCAGCC AACATTAGGT GAGGAGAGCA GCAGTCAGCA GGAGCAGGAG
    GAGCGTTTGC CTGACCTGCC GCTGTTAAGT GAGCAGCTGC TTTTGGATGA GCTGTGGGAC
    ATGCTTGGAG AATGTCTGAA AGAGCTTGAA GAGTCTCATG ACCAACATGC AGTTCTAGTG
    CTGCAGCCTG CAGTGGAGGC CTTTTTCTTG GTGCATGCAA CAGAGCGGGA AAGCAAACCA
    CCAGTTCGGG ACACTCGGGA GAGCCAGCTG TCACACATCA AGGATGAGCC TCCACCCCTG
    TCACCCGCCC CTCTCACACC CGCTACCCCC TCCTCTCTGG ACCCCTTTTT TTCCAGAGAG
    CCTTCCTCTA TGCACATCTC TTCCAACCTG CCTCCAGATA CACAGAAGTT CCTCCGTTTC
    GCAGAGACCC ACCGCACAGT GCTGAATCAG ATCCTGCGTC AATCCACCAC TCACCTGGCA
    GACGGGCCTT TCGCTGTTTT GGTTGACTAC ATTCGCATCT TGGACTTTGA CGTCAAGCGC
    AAGTATTTCC GTCAGGAGCT GGAGCGTCTG GATGAAGGCT TGAGGAAAGA GGATATGGCC
    GTCCATGTGA GGAGAGATCA CGTCTTTGAA GATTCTTACA GAGAGCTGCA CCGCAAGAGC
    CCAGAAGACA TGAAGAACAG ATTGTATATA GTATTTGAAG GAGAGGAGGG TCAGGATGCA
    GGCGGGCTGT TAAGAGAGTG GTACATGATC ATCTCCAGAG AAATGTTCAA CCCCATGTAT
    GCCCTGTTTC GCACCTCCCC CGGCGATCGG GTCACCTATA CCATCAACCC CTCCTCCCAC
    TGCAACCCCA ACCATCTCAG CTACTTCAAG TTTGTGGGAC GTGTGGTGGC CAAAGCAGTC
    TACGACAACA GGCTATTAGA GTGTTACTTT ACACGCAGCT TCTACAAGCA CATCCTGGGC
    AAGAGCGTTA GGTATACAGA CATGGAAAGC GAAGACTATC CCTTTTTCCA AGGTCTGGTT
    TATTTGTTGG AGAACGATGT GTCGACTCTG GGCTACGAGC TGACATTCAG CACAGAGGTC
    CAAGAGTTTG GCGTGTGTGA AGTGAGAGAT CTGAAGGCCA ACGGTGCCAA CATTCTTGTC
    ACAGAAGAGA ATAAGAAGGA ATATGTGCAT CTGGTCTGCC AGATGAAGAT GACAGGTGCA
    ATCCGTAAAC AGCTGGCTGC CTTCCTGGAG GGCTTTTATG AGATCATCCC CAAGAGACTG
    ATCTCCATCT TCACTGAGCA AGAGCTGGAG CTGCTCATCT CCGGCCTGCC CACCATTGAC
    ATCGACGACC TCAAGGCCAA TACTGAATAC CATAAATATC AGTCCAGTTC TATTCAGATC
    CAGTGGTTCT GGCGTGCTTT ACGATCCTTC GACCAGGCTG ACAGGGCCAA ATTTCTGCAG
    TTTGTTACGG GCACTTCCAA GGTGCCACTG CAGGGCTTTG CTGCACTGGA GGGAATGAAC
    GGCATTCAGA AGTTCCAGAT CCACCGAGAT GACCGCTCCA CTGACCGCCT GCCCTCTGCG
    CACACTTGCT TTAACCAGTT GGACCTCCCA GCGTACGAGA GCTACGAGAA GTTAAGACAC
    ATGCTGCTGC TGGCCATTCA GGAATGTTCT GAAGGCTTCG GACTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27859
    Clone ID Related Accession (Same CDS sequence) XM_005162282.3 , XM_021473710.1 , XM_005162282.4
    Accession Version XM_005162282.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162282.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
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    2281
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    2641
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    9781
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    10261
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    10501
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    11521
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    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAAGCAGCT
    CGCAGGCGTG AGGAGGAGGA GAGGAGAGCG AGGGAGCGGG TAGAGGAGGA AGAAGCTCGT
    TGTCTGGAGC GCTTTCTGGA AGCAGAGCCT CTTGACACCA CTGAGCTTCA TGCCTTCACA
    GACTCCATGC TGCCTGGCTG CTTCCACCTG CTCGATGAGC TGCCGGACAC AGTCTACCGC
    CTCTGTGATC TGCTCATGAC TGCTATTAAA AGAAACGGCC CTGAATACAG AGACCTCATC
    CTTAGACAAG TGGTCAATCA GGTGTGGGAG GCTGCTGACG TGTTGATAAA GGCTGCAGTG
    CCGCTAACCA CCAGCGACAC CAAGACAGTC TCTGAGTGGA CCAGACAGAT GGCGACCCTC
    CCTCAGGCCT CCAACCTGGC CACCCGCATC CTGCTGCTCA CGCTGCTCTT TGAGGAGCTG
    AAACTCTCCT GCGCTCGGGT GGTGGAGAGC AGTGGTGTCT TGACTGTGCT GATTAAGCTG
    TTGGAGGTGG TGCAGCCCTG TCTGCAAGCT GCCAAAGAGC AGAAGGACAT CCAAACGCCC
    AAATGGATCA CCCCTGTGCT CCTACTTATT GACTTTTATG AGAAAATGGC TGTTTCATCC
    AAACGAAGAG CTCAGATGAA CAAGTACCTC CAGCCCAATG GGAATAACTG GCGCTGGTTT
    GATGACCGTT CAGGCCGCTG GTGCAGCTAC AGTGCAAGTA ACAACAGCAC TATTGACTCT
    GCATGGAGGG CAGGAGAGAC CAGTGTCCGA TTTACAGCTG GCCGCAGAAG ATACACAGTT
    CAGTTCAACA CCATGGTGCA GGTAAATGAG GAGACTGGCA ACAGACGACC AGTTATGTTG
    ACGGTACAGC GGGTGCCACG CATTCCCAAG GCAACAAAAT CCGGAAGTGT TACTGACACG
    GAGAGAGAGG AGAGTGAACA GAGAGCAAAG ACTGAGGAAA CACAGACTGA CACAGATTCC
    AGCTCTGTGG CTGTAGAGAT GGCGCCCCCT AAAGAAGAAT CTGAGCTGAA GGATTCAACC
    CCAGCAGCTC CGTCAGTTCT CCAGGACCCC TCTGGTTCTA GTGGTATCGT TGTGCAGGGG
    CTGACAGAAG ACATGATCAC CATTTTAATC CGTGCCTGTG TCAGCATGAT CAGTGTTCCT
    GTGGACCCAG ACACTTTGCA CGCCACCTTG CGGCTCTGTC TGCGCCTCAC ACGCACACAC
    CATTACGCCA TGATGTTTGC AGAGCTCAAG AGCACCCGCA TGATTCTGAA TCTCACCCAG
    AGCTCAGGCT TTAATGGCTT CACCCCATTG GTCACCCTGC TCTTCCGCCA CATCATAGAG
    GACCCGGCCA CACTGCGGCA CACTATGGAG AAGGTGGTAC GTTCTGCAGT CACCAGTGGT
    GCAGGAAGCA CTACTTCAGG TGTGGTGTCA GGTAGTCTGG GCTCCAGGGA AATTAACTAC
    ATCCTGCGAG TCCTTGGCCC AGCGGCCTGT CGCAATCCAG AGTGCTTTAC GGAAACTGCC
    AAGAGTTGCA TCCGAATTGC TCTCCCGGCT CCAAGAGGTG CTGGCACTGC CTCAGATGAC
    GAGTTTGAGA ACTTCAGAAT TAAAGGTCCA AATGCTGTGC AACTCGTGAA GACCACCCCC
    CTAAAGCTCT CCCCGTTGCC CTCCATACCA GAGACTATAA AAGAGGTCAT CTATGACATG
    CTGAATGCCC TGGCAGCATA TCACGCACCA GAAGAGGCAG AGAGAGGAGA GGAGCGGGCA
    AACGCTGTAC CAGGGAATCA AGACCTGTGT CAGATCCTGC AGGATGTCGG TGATGATGTC
    TACCAGCAGT ACAGACTCAC CAGACAAGGC AGTGATTTTG ACTCGCAATC TGCCTTCCCC
    ATCAATACTC AGGTGTTTGC TGCAGATGGT GCTGTAGCTG AATCTTCTCA GTCTGGAACT
    CCACAGGGAG AAGCCTCTAC ACCCGAGGAA ATGCGTGAAG AGAAAAAGGA GCAGGAAGGA
    GACAAGGGAG CTTCTTCGGA GGAGAGCCGA GCTGCTAAGG CTAAAGCTAG CAAACCTCTG
    ATGCCCACCT CTACCATCTT GAGGCTGTTG GCCGAGCTGG TTCGCTCTTA TGTTGGCATT
    GCCACTCTTA TCACCTCTTA CAACTACACA GCTGGCCAGT CGGAGCTCAT TAAGGAAGAT
    TGCAGTGTGT TGGCATTTGT TTTGGACCAC TTGCTCCCAC ACACCCAGAA CTCTGAAGAT
    AAGGACACCC CTGCCCTTGC ACGCCTTTTC TTGGCCAGCC TTGCTGCAGC TGGCACGGGC
    ACCGATGCCC AGGCTGCACT GGTCAACGAG GTCAAAGCTG CTTTGAGTCG AGCATTGGGC
    ATGGCAGAGG GCACAGAGAA ACACGCCCGT TTGCAAGCAG TGATGTGCAT CATCAGCACC
    ATTATGGAGT CGTGTCCTTC CACCTCCAGC TTCTACAGCA CAGCCACAGC TAAGACCCAG
    CACAACGGCA TGAACAATAT CATCCGGCTC TTCTTGAAGA AAGGCCTTGT CAATGACCTG
    GCCCGTGTGC CTCACAGCCT TGATCTATCC AGTCCCAATA TGGCAAACAC AGTAAATGCT
    GCTTTGAAGC CTCTGGAAAC ATTGTCGCGA ATTGTGAACC AGCCCAGCAG CCTGTTTGGT
    GGTAAAGGGG GATCCAGCAA GAACAAGACT GAACATGACA CTGTTGGCAC AGTCAGAGAC
    AGTAACAGCA ACACTCAGGA TCAAGGAGAA GTGGGTGAGG CAGAGCCGGT TGAGAATCGT
    GACCGAGGTC AAGCCACAGA CTCTGACCTG ATGGATGGAG AAGCCGAGGG AGACACTGTG
    GTCATTGCTG GACAGCCTGA GGTGCTGAGC ACTCAGGCAA TGCAGGTGGA GAACGAGTTG
    GTGGATTTGA TCGATGAACT TTTAGAGAGA GATGCCCAAA CTGTTAATAG TACCATCATC
    GTAGGTCGTT CTGGAGAGGA TGAGTCTCAA GAGGATGTGC TGATGGATGA AGCTCCTTCC
    AATATGAGTC AGGCCTCCAC TCTCCAAGCC AACAGAGAAG ACTCCATGAA CATCCTCGAG
    CCAGAAGATG AGGAGCACAC GCAGGAGGAA GACTCCAGTG GTAGCAACGA GGATGAGGAC
    AGTCAGGATG AAGAGGAAGA GGAGGAGGAA GAAGAGGAGG AAGATCAGGA TGATGAAGAG
    GGAGATGAGG ATGATGACGA TGAAGGCTCA GAGATGGAAC TGGATGAAGA TTTTCCAGAT
    ATCAATGCTG CCCCTCATAT TCGTTTTGAG CGATTCGACC GAGATGATGA CCTCATCATT
    GAGTTTGATA ATATGTTCTC CACTTCAGCT GACATCCCTC CCTCACCAGG AAATATCCCC
    AGCTCCCACC CTCTGATGGT GCGTCACGCT GACCATGGCT CGCTGACTCT TGGGGGATCG
    GGCACCAGTA GCAGATTGGC TCAAGGAATG GGACGTAGTC AACGTTCTCT CCGCCAGCTC
    ACGGCCAACA CAGGCCACAC GATCCATGTA CATTATCCTG GCAACCGTCA GCCTAACCCT
    CCACTCATTC TGCAGAGATT ATTGGGCCCC AGCGCTGCAG CTGATATCTT GCAGCTGTCC
    AGCTCTCTTC CTCTGCAGTC TCGAGGTCGT GCCCGTTTAA TGGTGGGTAA TGAAGATGTG
    CACATTATTG CCCGTTCAGA CGATGAGCTG CTGGATGACT TCTTCCATGA GCAGAGCAGC
    ACCGGAGGAC AAGCTGGCAC CCTTTCAAGT ATCCCGACTG CCTTAACCAG ATGGACAGAT
    GAGTGTAAAG TGCTGGATGC TGAGAGCATG CATGACTGTG TGGCAGTGGT GAAGGTGCCC
    ATACTGCAGC ACCTGGAGAG TTTGCGTGAT GAGGAGCTGG AGGAACGCAG GGAGAAAAGG
    AGGAGGCAGT TGGCAGAGGA AGAGGAGACT GCAAAGCAGA ATGAGAGTTC TGCAGCAGGG
    GAGGAGACCA GGGAACAGAG TCTGCAGGGC TCTGGGATTG GAACAGTCAA TGGAGCTGCA
    GAGAATGCTG CAGAAGGAGA ACAGGCCCAG AGTGGGGTGT CCTCCTGTCT GGACCCTCCT
    CGCACCTCTG AGGGTTTCCT CACAGCGCCC CCATCAGGAG AAGTCACTCC AACCACCCCT
    GCCCCCCATG AACAAGCCCT CGTGTCTCTG GAAACAGCCA TCAGTCAGCA GGTGCACCAG
    CCAATCACAG AGCTGCTGCT GGCCGACTCC AATCCTGACT CTCACGGAGC CTTGGCAGGG
    GCAGGGCTTC CCCAGCTCTC AGCACCTGAT AGGCCAAATT GTGAGGCAGA AGCATCTCAG
    ATGGAGATGT CACCTGCACC CACTATTGGT GAGAGAGGAG GAGGAGCATT GGATGCAGTC
    AGGGAAGCTT CACTCAGTCC AGATATAGCT GAAAGTTCAG AGCCTGTGCC AGTGGGTGTG
    TCTCAACTCG AGGGTTCGCC CATGGATACG AGCAGTCCCG CTTCAGGAAC GCAGGAAGAG
    CCGGCTGCCA GCTCAGTTCC ATCCAATCAC ATGTCTCAGG AGCTATCAGG AAGTGGTGAC
    AGCGGCCTCA CAGACAGGCA GACAGATGCA GAAACTGGAT CAACTTCTGT TTCATCTCCT
    GGCGAGACCA TGCCTCGGAG TGACAGTGCC GATAGCCAGT CGCAGGCCAT CCAGGAGGAG
    CCCCTCCCTT CCACCAGCAA TGAAGAGGAG GACCCTCTGG CAGGCATCAG TCTACCTGAA
    GGCGTGGATC CGTCTTTCTT GGCAGCACTG CCTGAAGACA TTCGTCGGGA GGTGCTGCAG
    AACCAACTGG GAATCAGACC TCCTTCTCGA CCTCCTGTCG CCTCCACTCT GACAACCACT
    GCTAGTGCTG TGCTAGGAGG AGGGCCAGGG GTGACCGAAG TCAGCCCTGA ATTCTTAGCG
    GCTCTCCCAC CTGCAATTCA GGAAGAGGTC CTGGCCCAGC AGCGGGCAGA GCAGCAGCGT
    CGGGAGTTAT CCCAGCAACC CACACAGGGT GATCAACCTC TGGACCCAGT CACATTCATC
    CAGACCCTGC CCTCTGAGCT GCGGCGCTCC GTTCTGGAAG ACATGGAGGA CAGTGTACTA
    GCGGTCATGC CTCCAGATAT TGCAGCAGAG GCTGCAGCAC TGCGAAGGGA ACAAGAGGCC
    AGACAGAGAC AACTGATGCA CGAGCGCTTG TTCGGTCACA GCTCCTCTTC AGCTCTGTCT
    GCCATCTTAC GCTCTCCCGC CTTCACCAGC CGCCTCGGGG GCAACCGTGG GGTACAGTAC
    ACCCGGCTGG CTGTGCAAAG AGGAGGGACG TTTCAAATGG GAGGGAGTGC CAATCATAGC
    AGACCCTCTA GTTCAAACGT TGACTCTCTC CTGCGCCTGC GGGGGCGTCT CTTGTTGGAC
    CACGAGGCCT TGTCCTGCCT TCTTGTGCTG CTTTTTGTGG ACGAGCCCAA ACTTAACACC
    AGCCGCCTCC ACCGAGTGCT GCGTAACTTG TGCTATCACT CTCAGACGCG TGGCTGGGTC
    ATCCGCAGTC TCCTGTCCAT CCTGCAGCGC AGCAGTGAGA GCGAGCTGTG CCTGGAGACT
    GCACGCTTGG AGGAGTCCCG TGGCAAACGC TCACTAGGCC AGGGCTCATC TAGTGGAGGA
    AAGGGTTGTT CTTCATCCTC CCAGTCGGCT ATAGCCTCTT CGTCATCGTC CTCCTCGTCC
    TCTTCTTCTC TGGAGCTGCT GAACAGGGTG GAGTCACGGA GTTCCAGTCA GCTCTCCTGG
    CTGTCTGTAT CAATGGACGC AGCTCTTGGA TGCAGAACCA ACATCTTTCA GATCCAGCGA
    GTATCAGGCA GGAAACACGC AGAGAGGCAT TCAGCAGGAG GGAGTGTGAG CGCAGGAGGA
    CTCACAGGTG GAGTGTCAGG AACAGGAATG ACGTGTACAG GGGGAGGTGG GTCAACTGTT
    CACATCCATC CACAGGCTGC GCCGGTGGTC TGTCGACATG TACTGGACAC CCTCATCCAA
    TTGGCCAAGG TATTTCCAAG TCACTTCACT CAACAACGCT GTAAAGATCC CCTATCCTCC
    TCCTCCTCTG AGTTGGACAC TCGTCTTTGT GCCAGCTCAA CACCGACTAT GAGTGCCAGT
    TGTCGGTCAG GTGGCACCAG TCAGAGTAAC TCCTCTGCTA CCCCAAGTAG TCTCACTTCT
    ATCCACAATT CCCTGAGCGC CTGCTCTGGT ACGCCACAGT CCCTAGGCAT CTCCACCGAC
    TTTTGGGATC TCCTGGTCAA GCTGGACAAC ATGAATGTGA GCCGAAAGGG GAAGGCCTCC
    ATGAAGACTG TCCCTCTAGG GGGTGTCGGC GAGACAGAGG GTGCGCTGTT CAGCCTTGAG
    GCGTCTCCGC TGGGCCAGCT GATGAATATG CTGTCCCACC CTGTGATCCG CCGTAGTTCC
    CTCCTCACGG AGAAACTGCT ACGTCTGCTG TCTCTGATCT CCATCGCTCT GCCTGACAAC
    AAGGCCACGG AGGTTCCAGC CAGCCATCCA GTGCCTCAGA CCCCAACTGC CACGCCATCT
    GCACCTGCTC CTCAAACCAC AGCAGGGTCA GGGACCATCT CAGCAGTGGC AGCTACAGGC
    ACTACGCAGG GTTCAGTCGG TTCAGTGGGT ACAACGCCAT CTCAACCAGC CGCTACAATA
    TCTGTGGTTG CAGGCAGCAC CACCGCCTCT AGCATTATTG CTGGAAAACT GAAGCCAGCA
    GTAAGCATCA CGGACATTGA ATCTAAACTG GCCTCTACTG GACTTACGGA GAAGCAGCTG
    CAGCTCTCTG TGGAGGTTCT GACATCTCAC TCGTGTTCAG AGGAAGGTCT GGAGGACGCA
    GCCAACATCT TGCTGCAGCT GTCCAGAGGG GACGCACCGA CCCGAGACAC TGTGCTTAGA
    CTTCTGCTCA GCGGAGCACG TCACCTTGGA TACACTCTCT GCAAACAAAT CGGCACTTTA
    TTAGCTGAGT TGAGGGAGTA TAATCTAGAG CAGCAGAGAA GAGCACGGGC TGATGCTCAG
    TCTCCTGAAG CTTCTCATGA TGACCCATCC ATCACCACAC GCCTCAAGGG CAAGATGACC
    AGCAGGTTTG ACGGGTCAGA GAGTGTGGTG ATTGTTGCTG CTCAGAAGCG CACGTTAGGT
    GGCCGGGAGC TTCAGTTGCC CTGCATGAGC TCGCTCACTT CCAAGACCTC AACGCAGAAG
    TTTTTCTTGC GGGTACTGCA GGTCATCATC CAGCTTAGAG AAGACACGCG GCGCGCTAAC
    AAGAAAGCCA AGCAGACAGG AGGAAGACTA AGTTCAACCA GTCTGGGCTC GGCCAGTAGT
    ATTCAGGCTG CGGTCCGTCA GCTGGAGGCT GAAGCGGACG CCATCATTCA GATGGTGAGA
    GAGGGACAGA GGGCACGCCG TCTACAGCAG GCCCCCCCAC CCTCTTCATC TGCATCCTCT
    TCCGCTGCTG CTGCTGCTGC ATCCGCTGCT GTGGCTGTGG CTGGATCGTC TGCCCCCAGT
    ACCTCTGCAA CCAACAACCC CCCCGCTGGC ACGGCCAGCG CAGCCACGTC GGAGGTTCAC
    ACTGTTGCCG AGCCACCAGC AGCTCAGAGA GATGATTCGC CCATGGATGT GGATCAGCCC
    TCTCCTTTGG AACAGGACCA GCCAACATTA GGTGAGGAGA GCAGCAGTCA GCAGGAGCAG
    GAGGAGCGTT TGCCTGACCT GCCGCTGTTA AGTGAGCAGC TGCTTTTGGA TGAGCTGTGG
    GACATGCTTG GAGAATGTCT GAAAGAGCTT GAAGAGTCTC ATGACCAACA TGCAGTTCTA
    GTGCTGCAGC CTGCAGTGGA GGCCTTTTTC TTGGTGCATG CAACAGAGCG GGAAAGCAAA
    CCACCAGTTC GGGACACTCG GGAGAGCCAG CTGTCACACA TCAAGGATGA GCCTCCACCC
    CTGTCACCCG CCCCTCTCAC ACCCGCTACC CCCTCCTCTC TGGACCCCTT TTTTTCCAGA
    GAGCCTTCCT CTATGCACAT CTCTTCCAAC CTGCCTCCAG ATACACAGAA GTTCCTCCGT
    TTCGCAGAGA CCCACCGCAC AGTGCTGAAT CAGATCCTGC GTCAATCCAC CACTCACCTG
    GCAGACGGGC CTTTCGCTGT TTTGGTTGAC TACATTCGCA TCTTGGACTT TGACGTCAAG
    CGCAAGTATT TCCGTCAGGA GCTGGAGCGT CTGGATGAAG GCTTGAGGAA AGAGGATATG
    GCCGTCCATG TGAGGAGAGA TCACGTCTTT GAAGATTCTT ACAGAGAGCT GCACCGCAAG
    AGCCCAGAAG ACATGAAGAA CAGATTGTAT ATAGTATTTG AAGGAGAGGA GGGTCAGGAT
    GCAGGCGGGC TGTTAAGAGA GTGGTACATG ATCATCTCCA GAGAAATGTT CAACCCCATG
    TATGCCCTGT TTCGCACCTC CCCCGGCGAT CGGGTCACCT ATACCATCAA CCCCTCCTCC
    CACTGCAACC CCAACCATCT CAGCTACTTC AAGTTTGTGG GACGTGTGGT GGCCAAAGCA
    GTCTACGACA ACAGGCTATT AGAGTGTTAC TTTACACGCA GCTTCTACAA GCACATCCTG
    GGCAAGAGCG TTAGGTATAC AGACATGGAA AGCGAAGACT ATCCCTTTTT CCAAGGTCTG
    GTTTATTTGT TGGAGAACGA TGTGTCGACT CTGGGCTACG AGCTGACATT CAGCACAGAG
    GTCCAAGAGT TTGGCGTGTG TGAAGTGAGA GATCTGAAGG CCAACGGTGC CAACATTCTT
    GTCACAGAAG AGAATAAGAA GGAATATGTG CATCTGGTCT GCCAGATGAA GATGACAGGT
    GCAATCCGTA AACAGCTGGC TGCCTTCCTG GAGGGCTTTT ATGAGATCAT CCCCAAGAGA
    CTGATCTCCA TCTTCACTGA GCAAGAGCTG GAGCTGCTCA TCTCCGGCCT GCCCACCATT
    GACATCGACG ACCTCAAGGC CAATACTGAA TACCATAAAT ATCAGTCCAG TTCTATTCAG
    ATCCAGTGGT TCTGGCGTGC TTTACGATCC TTCGACCAGG CTGACAGGGC CAAATTTCTG
    CAGTTTGTTA CGGGCACTTC CAAGGTGCCA CTGCAGGGCT TTGCTGCACT GGAGGGAATG
    AACGGCATTC AGAAGTTCCA GATCCACCGA GATGACCGCT CCACTGACCG CCTGCCCTCT
    GCGCACACTT GCTTTAACCA GTTGGACCTC CCAGCGTACG AGAGCTACGA GAAGTTAAGA
    CACATGCTGC TGCTGGCCAT TCAGGAATGT TCTGAAGGCT TCGGACTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162339.1
    CDS149..13762
    Translation

    Target ORF information:

    RefSeq Version XM_005162282.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162282.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAAGCAGCT
    CGCAGGCGTG AGGAGGAGGA GAGGAGAGCG AGGGAGCGGG TAGAGGAGGA AGAAGCTCGT
    TGTCTGGAGC GCTTTCTGGA AGCAGAGCCT CTTGACACCA CTGAGCTTCA TGCCTTCACA
    GACTCCATGC TGCCTGGCTG CTTCCACCTG CTCGATGAGC TGCCGGACAC AGTCTACCGC
    CTCTGTGATC TGCTCATGAC TGCTATTAAA AGAAACGGCC CTGAATACAG AGACCTCATC
    CTTAGACAAG TGGTCAATCA GGTGTGGGAG GCTGCTGACG TGTTGATAAA GGCTGCAGTG
    CCGCTAACCA CCAGCGACAC CAAGACAGTC TCTGAGTGGA CCAGACAGAT GGCGACCCTC
    CCTCAGGCCT CCAACCTGGC CACCCGCATC CTGCTGCTCA CGCTGCTCTT TGAGGAGCTG
    AAACTCTCCT GCGCTCGGGT GGTGGAGAGC AGTGGTGTCT TGACTGTGCT GATTAAGCTG
    TTGGAGGTGG TGCAGCCCTG TCTGCAAGCT GCCAAAGAGC AGAAGGACAT CCAAACGCCC
    AAATGGATCA CCCCTGTGCT CCTACTTATT GACTTTTATG AGAAAATGGC TGTTTCATCC
    AAACGAAGAG CTCAGATGAA CAAGTACCTC CAGCCCAATG GGAATAACTG GCGCTGGTTT
    GATGACCGTT CAGGCCGCTG GTGCAGCTAC AGTGCAAGTA ACAACAGCAC TATTGACTCT
    GCATGGAGGG CAGGAGAGAC CAGTGTCCGA TTTACAGCTG GCCGCAGAAG ATACACAGTT
    CAGTTCAACA CCATGGTGCA GGTAAATGAG GAGACTGGCA ACAGACGACC AGTTATGTTG
    ACGGTACAGC GGGTGCCACG CATTCCCAAG GCAACAAAAT CCGGAAGTGT TACTGACACG
    GAGAGAGAGG AGAGTGAACA GAGAGCAAAG ACTGAGGAAA CACAGACTGA CACAGATTCC
    AGCTCTGTGG CTGTAGAGAT GGCGCCCCCT AAAGAAGAAT CTGAGCTGAA GGATTCAACC
    CCAGCAGCTC CGTCAGTTCT CCAGGACCCC TCTGGTTCTA GTGGTATCGT TGTGCAGGGG
    CTGACAGAAG ACATGATCAC CATTTTAATC CGTGCCTGTG TCAGCATGAT CAGTGTTCCT
    GTGGACCCAG ACACTTTGCA CGCCACCTTG CGGCTCTGTC TGCGCCTCAC ACGCACACAC
    CATTACGCCA TGATGTTTGC AGAGCTCAAG AGCACCCGCA TGATTCTGAA TCTCACCCAG
    AGCTCAGGCT TTAATGGCTT CACCCCATTG GTCACCCTGC TCTTCCGCCA CATCATAGAG
    GACCCGGCCA CACTGCGGCA CACTATGGAG AAGGTGGTAC GTTCTGCAGT CACCAGTGGT
    GCAGGAAGCA CTACTTCAGG TGTGGTGTCA GGTAGTCTGG GCTCCAGGGA AATTAACTAC
    ATCCTGCGAG TCCTTGGCCC AGCGGCCTGT CGCAATCCAG AGTGCTTTAC GGAAACTGCC
    AAGAGTTGCA TCCGAATTGC TCTCCCGGCT CCAAGAGGTG CTGGCACTGC CTCAGATGAC
    GAGTTTGAGA ACTTCAGAAT TAAAGGTCCA AATGCTGTGC AACTCGTGAA GACCACCCCC
    CTAAAGCTCT CCCCGTTGCC CTCCATACCA GAGACTATAA AAGAGGTCAT CTATGACATG
    CTGAATGCCC TGGCAGCATA TCACGCACCA GAAGAGGCAG AGAGAGGAGA GGAGCGGGCA
    AACGCTGTAC CAGGGAATCA AGACCTGTGT CAGATCCTGC AGGATGTCGG TGATGATGTC
    TACCAGCAGT ACAGACTCAC CAGACAAGGC AGTGATTTTG ACTCGCAATC TGCCTTCCCC
    ATCAATACTC AGGTGTTTGC TGCAGATGGT GCTGTAGCTG AATCTTCTCA GTCTGGAACT
    CCACAGGGAG AAGCCTCTAC ACCCGAGGAA ATGCGTGAAG AGAAAAAGGA GCAGGAAGGA
    GACAAGGGAG CTTCTTCGGA GGAGAGCCGA GCTGCTAAGG CTAAAGCTAG CAAACCTCTG
    ATGCCCACCT CTACCATCTT GAGGCTGTTG GCCGAGCTGG TTCGCTCTTA TGTTGGCATT
    GCCACTCTTA TCACCTCTTA CAACTACACA GCTGGCCAGT CGGAGCTCAT TAAGGAAGAT
    TGCAGTGTGT TGGCATTTGT TTTGGACCAC TTGCTCCCAC ACACCCAGAA CTCTGAAGAT
    AAGGACACCC CTGCCCTTGC ACGCCTTTTC TTGGCCAGCC TTGCTGCAGC TGGCACGGGC
    ACCGATGCCC AGGCTGCACT GGTCAACGAG GTCAAAGCTG CTTTGAGTCG AGCATTGGGC
    ATGGCAGAGG GCACAGAGAA ACACGCCCGT TTGCAAGCAG TGATGTGCAT CATCAGCACC
    ATTATGGAGT CGTGTCCTTC CACCTCCAGC TTCTACAGCA CAGCCACAGC TAAGACCCAG
    CACAACGGCA TGAACAATAT CATCCGGCTC TTCTTGAAGA AAGGCCTTGT CAATGACCTG
    GCCCGTGTGC CTCACAGCCT TGATCTATCC AGTCCCAATA TGGCAAACAC AGTAAATGCT
    GCTTTGAAGC CTCTGGAAAC ATTGTCGCGA ATTGTGAACC AGCCCAGCAG CCTGTTTGGT
    GGTAAAGGGG GATCCAGCAA GAACAAGACT GAACATGACA CTGTTGGCAC AGTCAGAGAC
    AGTAACAGCA ACACTCAGGA TCAAGGAGAA GTGGGTGAGG CAGAGCCGGT TGAGAATCGT
    GACCGAGGTC AAGCCACAGA CTCTGACCTG ATGGATGGAG AAGCCGAGGG AGACACTGTG
    GTCATTGCTG GACAGCCTGA GGTGCTGAGC ACTCAGGCAA TGCAGGTGGA GAACGAGTTG
    GTGGATTTGA TCGATGAACT TTTAGAGAGA GATGCCCAAA CTGTTAATAG TACCATCATC
    GTAGGTCGTT CTGGAGAGGA TGAGTCTCAA GAGGATGTGC TGATGGATGA AGCTCCTTCC
    AATATGAGTC AGGCCTCCAC TCTCCAAGCC AACAGAGAAG ACTCCATGAA CATCCTCGAG
    CCAGAAGATG AGGAGCACAC GCAGGAGGAA GACTCCAGTG GTAGCAACGA GGATGAGGAC
    AGTCAGGATG AAGAGGAAGA GGAGGAGGAA GAAGAGGAGG AAGATCAGGA TGATGAAGAG
    GGAGATGAGG ATGATGACGA TGAAGGCTCA GAGATGGAAC TGGATGAAGA TTTTCCAGAT
    ATCAATGCTG CCCCTCATAT TCGTTTTGAG CGATTCGACC GAGATGATGA CCTCATCATT
    GAGTTTGATA ATATGTTCTC CACTTCAGCT GACATCCCTC CCTCACCAGG AAATATCCCC
    AGCTCCCACC CTCTGATGGT GCGTCACGCT GACCATGGCT CGCTGACTCT TGGGGGATCG
    GGCACCAGTA GCAGATTGGC TCAAGGAATG GGACGTAGTC AACGTTCTCT CCGCCAGCTC
    ACGGCCAACA CAGGCCACAC GATCCATGTA CATTATCCTG GCAACCGTCA GCCTAACCCT
    CCACTCATTC TGCAGAGATT ATTGGGCCCC AGCGCTGCAG CTGATATCTT GCAGCTGTCC
    AGCTCTCTTC CTCTGCAGTC TCGAGGTCGT GCCCGTTTAA TGGTGGGTAA TGAAGATGTG
    CACATTATTG CCCGTTCAGA CGATGAGCTG CTGGATGACT TCTTCCATGA GCAGAGCAGC
    ACCGGAGGAC AAGCTGGCAC CCTTTCAAGT ATCCCGACTG CCTTAACCAG ATGGACAGAT
    GAGTGTAAAG TGCTGGATGC TGAGAGCATG CATGACTGTG TGGCAGTGGT GAAGGTGCCC
    ATACTGCAGC ACCTGGAGAG TTTGCGTGAT GAGGAGCTGG AGGAACGCAG GGAGAAAAGG
    AGGAGGCAGT TGGCAGAGGA AGAGGAGACT GCAAAGCAGA ATGAGAGTTC TGCAGCAGGG
    GAGGAGACCA GGGAACAGAG TCTGCAGGGC TCTGGGATTG GAACAGTCAA TGGAGCTGCA
    GAGAATGCTG CAGAAGGAGA ACAGGCCCAG AGTGGGGTGT CCTCCTGTCT GGACCCTCCT
    CGCACCTCTG AGGGTTTCCT CACAGCGCCC CCATCAGGAG AAGTCACTCC AACCACCCCT
    GCCCCCCATG AACAAGCCCT CGTGTCTCTG GAAACAGCCA TCAGTCAGCA GGTGCACCAG
    CCAATCACAG AGCTGCTGCT GGCCGACTCC AATCCTGACT CTCACGGAGC CTTGGCAGGG
    GCAGGGCTTC CCCAGCTCTC AGCACCTGAT AGGCCAAATT GTGAGGCAGA AGCATCTCAG
    ATGGAGATGT CACCTGCACC CACTATTGGT GAGAGAGGAG GAGGAGCATT GGATGCAGTC
    AGGGAAGCTT CACTCAGTCC AGATATAGCT GAAAGTTCAG AGCCTGTGCC AGTGGGTGTG
    TCTCAACTCG AGGGTTCGCC CATGGATACG AGCAGTCCCG CTTCAGGAAC GCAGGAAGAG
    CCGGCTGCCA GCTCAGTTCC ATCCAATCAC ATGTCTCAGG AGCTATCAGG AAGTGGTGAC
    AGCGGCCTCA CAGACAGGCA GACAGATGCA GAAACTGGAT CAACTTCTGT TTCATCTCCT
    GGCGAGACCA TGCCTCGGAG TGACAGTGCC GATAGCCAGT CGCAGGCCAT CCAGGAGGAG
    CCCCTCCCTT CCACCAGCAA TGAAGAGGAG GACCCTCTGG CAGGCATCAG TCTACCTGAA
    GGCGTGGATC CGTCTTTCTT GGCAGCACTG CCTGAAGACA TTCGTCGGGA GGTGCTGCAG
    AACCAACTGG GAATCAGACC TCCTTCTCGA CCTCCTGTCG CCTCCACTCT GACAACCACT
    GCTAGTGCTG TGCTAGGAGG AGGGCCAGGG GTGACCGAAG TCAGCCCTGA ATTCTTAGCG
    GCTCTCCCAC CTGCAATTCA GGAAGAGGTC CTGGCCCAGC AGCGGGCAGA GCAGCAGCGT
    CGGGAGTTAT CCCAGCAACC CACACAGGGT GATCAACCTC TGGACCCAGT CACATTCATC
    CAGACCCTGC CCTCTGAGCT GCGGCGCTCC GTTCTGGAAG ACATGGAGGA CAGTGTACTA
    GCGGTCATGC CTCCAGATAT TGCAGCAGAG GCTGCAGCAC TGCGAAGGGA ACAAGAGGCC
    AGACAGAGAC AACTGATGCA CGAGCGCTTG TTCGGTCACA GCTCCTCTTC AGCTCTGTCT
    GCCATCTTAC GCTCTCCCGC CTTCACCAGC CGCCTCGGGG GCAACCGTGG GGTACAGTAC
    ACCCGGCTGG CTGTGCAAAG AGGAGGGACG TTTCAAATGG GAGGGAGTGC CAATCATAGC
    AGACCCTCTA GTTCAAACGT TGACTCTCTC CTGCGCCTGC GGGGGCGTCT CTTGTTGGAC
    CACGAGGCCT TGTCCTGCCT TCTTGTGCTG CTTTTTGTGG ACGAGCCCAA ACTTAACACC
    AGCCGCCTCC ACCGAGTGCT GCGTAACTTG TGCTATCACT CTCAGACGCG TGGCTGGGTC
    ATCCGCAGTC TCCTGTCCAT CCTGCAGCGC AGCAGTGAGA GCGAGCTGTG CCTGGAGACT
    GCACGCTTGG AGGAGTCCCG TGGCAAACGC TCACTAGGCC AGGGCTCATC TAGTGGAGGA
    AAGGGTTGTT CTTCATCCTC CCAGTCGGCT ATAGCCTCTT CGTCATCGTC CTCCTCGTCC
    TCTTCTTCTC TGGAGCTGCT GAACAGGGTG GAGTCACGGA GTTCCAGTCA GCTCTCCTGG
    CTGTCTGTAT CAATGGACGC AGCTCTTGGA TGCAGAACCA ACATCTTTCA GATCCAGCGA
    GTATCAGGCA GGAAACACGC AGAGAGGCAT TCAGCAGGAG GGAGTGTGAG CGCAGGAGGA
    CTCACAGGTG GAGTGTCAGG AACAGGAATG ACGTGTACAG GGGGAGGTGG GTCAACTGTT
    CACATCCATC CACAGGCTGC GCCGGTGGTC TGTCGACATG TACTGGACAC CCTCATCCAA
    TTGGCCAAGG TATTTCCAAG TCACTTCACT CAACAACGCT GTAAAGATCC CCTATCCTCC
    TCCTCCTCTG AGTTGGACAC TCGTCTTTGT GCCAGCTCAA CACCGACTAT GAGTGCCAGT
    TGTCGGTCAG GTGGCACCAG TCAGAGTAAC TCCTCTGCTA CCCCAAGTAG TCTCACTTCT
    ATCCACAATT CCCTGAGCGC CTGCTCTGGT ACGCCACAGT CCCTAGGCAT CTCCACCGAC
    TTTTGGGATC TCCTGGTCAA GCTGGACAAC ATGAATGTGA GCCGAAAGGG GAAGGCCTCC
    ATGAAGACTG TCCCTCTAGG GGGTGTCGGC GAGACAGAGG GTGCGCTGTT CAGCCTTGAG
    GCGTCTCCGC TGGGCCAGCT GATGAATATG CTGTCCCACC CTGTGATCCG CCGTAGTTCC
    CTCCTCACGG AGAAACTGCT ACGTCTGCTG TCTCTGATCT CCATCGCTCT GCCTGACAAC
    AAGGCCACGG AGGTTCCAGC CAGCCATCCA GTGCCTCAGA CCCCAACTGC CACGCCATCT
    GCACCTGCTC CTCAAACCAC AGCAGGGTCA GGGACCATCT CAGCAGTGGC AGCTACAGGC
    ACTACGCAGG GTTCAGTCGG TTCAGTGGGT ACAACGCCAT CTCAACCAGC CGCTACAATA
    TCTGTGGTTG CAGGCAGCAC CACCGCCTCT AGCATTATTG CTGGAAAACT GAAGCCAGCA
    GTAAGCATCA CGGACATTGA ATCTAAACTG GCCTCTACTG GACTTACGGA GAAGCAGCTG
    CAGCTCTCTG TGGAGGTTCT GACATCTCAC TCGTGTTCAG AGGAAGGTCT GGAGGACGCA
    GCCAACATCT TGCTGCAGCT GTCCAGAGGG GACGCACCGA CCCGAGACAC TGTGCTTAGA
    CTTCTGCTCA GCGGAGCACG TCACCTTGGA TACACTCTCT GCAAACAAAT CGGCACTTTA
    TTAGCTGAGT TGAGGGAGTA TAATCTAGAG CAGCAGAGAA GAGCACGGGC TGATGCTCAG
    TCTCCTGAAG CTTCTCATGA TGACCCATCC ATCACCACAC GCCTCAAGGG CAAGATGACC
    AGCAGGTTTG ACGGGTCAGA GAGTGTGGTG ATTGTTGCTG CTCAGAAGCG CACGTTAGGT
    GGCCGGGAGC TTCAGTTGCC CTGCATGAGC TCGCTCACTT CCAAGACCTC AACGCAGAAG
    TTTTTCTTGC GGGTACTGCA GGTCATCATC CAGCTTAGAG AAGACACGCG GCGCGCTAAC
    AAGAAAGCCA AGCAGACAGG AGGAAGACTA AGTTCAACCA GTCTGGGCTC GGCCAGTAGT
    ATTCAGGCTG CGGTCCGTCA GCTGGAGGCT GAAGCGGACG CCATCATTCA GATGGTGAGA
    GAGGGACAGA GGGCACGCCG TCTACAGCAG GCCCCCCCAC CCTCTTCATC TGCATCCTCT
    TCCGCTGCTG CTGCTGCTGC ATCCGCTGCT GTGGCTGTGG CTGGATCGTC TGCCCCCAGT
    ACCTCTGCAA CCAACAACCC CCCCGCTGGC ACGGCCAGCG CAGCCACGTC GGAGGTTCAC
    ACTGTTGCCG AGCCACCAGC AGCTCAGAGA GATGATTCGC CCATGGATGT GGATCAGCCC
    TCTCCTTTGG AACAGGACCA GCCAACATTA GGTGAGGAGA GCAGCAGTCA GCAGGAGCAG
    GAGGAGCGTT TGCCTGACCT GCCGCTGTTA AGTGAGCAGC TGCTTTTGGA TGAGCTGTGG
    GACATGCTTG GAGAATGTCT GAAAGAGCTT GAAGAGTCTC ATGACCAACA TGCAGTTCTA
    GTGCTGCAGC CTGCAGTGGA GGCCTTTTTC TTGGTGCATG CAACAGAGCG GGAAAGCAAA
    CCACCAGTTC GGGACACTCG GGAGAGCCAG CTGTCACACA TCAAGGATGA GCCTCCACCC
    CTGTCACCCG CCCCTCTCAC ACCCGCTACC CCCTCCTCTC TGGACCCCTT TTTTTCCAGA
    GAGCCTTCCT CTATGCACAT CTCTTCCAAC CTGCCTCCAG ATACACAGAA GTTCCTCCGT
    TTCGCAGAGA CCCACCGCAC AGTGCTGAAT CAGATCCTGC GTCAATCCAC CACTCACCTG
    GCAGACGGGC CTTTCGCTGT TTTGGTTGAC TACATTCGCA TCTTGGACTT TGACGTCAAG
    CGCAAGTATT TCCGTCAGGA GCTGGAGCGT CTGGATGAAG GCTTGAGGAA AGAGGATATG
    GCCGTCCATG TGAGGAGAGA TCACGTCTTT GAAGATTCTT ACAGAGAGCT GCACCGCAAG
    AGCCCAGAAG ACATGAAGAA CAGATTGTAT ATAGTATTTG AAGGAGAGGA GGGTCAGGAT
    GCAGGCGGGC TGTTAAGAGA GTGGTACATG ATCATCTCCA GAGAAATGTT CAACCCCATG
    TATGCCCTGT TTCGCACCTC CCCCGGCGAT CGGGTCACCT ATACCATCAA CCCCTCCTCC
    CACTGCAACC CCAACCATCT CAGCTACTTC AAGTTTGTGG GACGTGTGGT GGCCAAAGCA
    GTCTACGACA ACAGGCTATT AGAGTGTTAC TTTACACGCA GCTTCTACAA GCACATCCTG
    GGCAAGAGCG TTAGGTATAC AGACATGGAA AGCGAAGACT ATCCCTTTTT CCAAGGTCTG
    GTTTATTTGT TGGAGAACGA TGTGTCGACT CTGGGCTACG AGCTGACATT CAGCACAGAG
    GTCCAAGAGT TTGGCGTGTG TGAAGTGAGA GATCTGAAGG CCAACGGTGC CAACATTCTT
    GTCACAGAAG AGAATAAGAA GGAATATGTG CATCTGGTCT GCCAGATGAA GATGACAGGT
    GCAATCCGTA AACAGCTGGC TGCCTTCCTG GAGGGCTTTT ATGAGATCAT CCCCAAGAGA
    CTGATCTCCA TCTTCACTGA GCAAGAGCTG GAGCTGCTCA TCTCCGGCCT GCCCACCATT
    GACATCGACG ACCTCAAGGC CAATACTGAA TACCATAAAT ATCAGTCCAG TTCTATTCAG
    ATCCAGTGGT TCTGGCGTGC TTTACGATCC TTCGACCAGG CTGACAGGGC CAAATTTCTG
    CAGTTTGTTA CGGGCACTTC CAAGGTGCCA CTGCAGGGCT TTGCTGCACT GGAGGGAATG
    AACGGCATTC AGAAGTTCCA GATCCACCGA GATGACCGCT CCACTGACCG CCTGCCCTCT
    GCGCACACTT GCTTTAACCA GTTGGACCTC CCAGCGTACG AGAGCTACGA GAAGTTAAGA
    CACATGCTGC TGCTGGCCAT TCAGGAATGT TCTGAAGGCT TCGGACTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27859
    Clone ID Related Accession (Same CDS sequence) XM_005162282.3 , XM_021473710.1 , XM_005162282.4
    Accession Version XM_021473710.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    541
    601
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    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
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    3121
    3181
    3241
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    3481
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    4141
    4201
    4261
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    4381
    4441
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    4681
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    4921
    4981
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    5401
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    5521
    5581
    5641
    5701
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    5881
    5941
    6001
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    6121
    6181
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    6301
    6361
    6421
    6481
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    7141
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    9301
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    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
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    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAAGCAGCT
    CGCAGGCGTG AGGAGGAGGA GAGGAGAGCG AGGGAGCGGG TAGAGGAGGA AGAAGCTCGT
    TGTCTGGAGC GCTTTCTGGA AGCAGAGCCT CTTGACACCA CTGAGCTTCA TGCCTTCACA
    GACTCCATGC TGCCTGGCTG CTTCCACCTG CTCGATGAGC TGCCGGACAC AGTCTACCGC
    CTCTGTGATC TGCTCATGAC TGCTATTAAA AGAAACGGCC CTGAATACAG AGACCTCATC
    CTTAGACAAG TGGTCAATCA GGTGTGGGAG GCTGCTGACG TGTTGATAAA GGCTGCAGTG
    CCGCTAACCA CCAGCGACAC CAAGACAGTC TCTGAGTGGA CCAGACAGAT GGCGACCCTC
    CCTCAGGCCT CCAACCTGGC CACCCGCATC CTGCTGCTCA CGCTGCTCTT TGAGGAGCTG
    AAACTCTCCT GCGCTCGGGT GGTGGAGAGC AGTGGTGTCT TGACTGTGCT GATTAAGCTG
    TTGGAGGTGG TGCAGCCCTG TCTGCAAGCT GCCAAAGAGC AGAAGGACAT CCAAACGCCC
    AAATGGATCA CCCCTGTGCT CCTACTTATT GACTTTTATG AGAAAATGGC TGTTTCATCC
    AAACGAAGAG CTCAGATGAA CAAGTACCTC CAGCCCAATG GGAATAACTG GCGCTGGTTT
    GATGACCGTT CAGGCCGCTG GTGCAGCTAC AGTGCAAGTA ACAACAGCAC TATTGACTCT
    GCATGGAGGG CAGGAGAGAC CAGTGTCCGA TTTACAGCTG GCCGCAGAAG ATACACAGTT
    CAGTTCAACA CCATGGTGCA GGTAAATGAG GAGACTGGCA ACAGACGACC AGTTATGTTG
    ACGGTACAGC GGGTGCCACG CATTCCCAAG GCAACAAAAT CCGGAAGTGT TACTGACACG
    GAGAGAGAGG AGAGTGAACA GAGAGCAAAG ACTGAGGAAA CACAGACTGA CACAGATTCC
    AGCTCTGTGG CTGTAGAGAT GGCGCCCCCT AAAGAAGAAT CTGAGCTGAA GGATTCAACC
    CCAGCAGCTC CGTCAGTTCT CCAGGACCCC TCTGGTTCTA GTGGTATCGT TGTGCAGGGG
    CTGACAGAAG ACATGATCAC CATTTTAATC CGTGCCTGTG TCAGCATGAT CAGTGTTCCT
    GTGGACCCAG ACACTTTGCA CGCCACCTTG CGGCTCTGTC TGCGCCTCAC ACGCACACAC
    CATTACGCCA TGATGTTTGC AGAGCTCAAG AGCACCCGCA TGATTCTGAA TCTCACCCAG
    AGCTCAGGCT TTAATGGCTT CACCCCATTG GTCACCCTGC TCTTCCGCCA CATCATAGAG
    GACCCGGCCA CACTGCGGCA CACTATGGAG AAGGTGGTAC GTTCTGCAGT CACCAGTGGT
    GCAGGAAGCA CTACTTCAGG TGTGGTGTCA GGTAGTCTGG GCTCCAGGGA AATTAACTAC
    ATCCTGCGAG TCCTTGGCCC AGCGGCCTGT CGCAATCCAG AGTGCTTTAC GGAAACTGCC
    AAGAGTTGCA TCCGAATTGC TCTCCCGGCT CCAAGAGGTG CTGGCACTGC CTCAGATGAC
    GAGTTTGAGA ACTTCAGAAT TAAAGGTCCA AATGCTGTGC AACTCGTGAA GACCACCCCC
    CTAAAGCTCT CCCCGTTGCC CTCCATACCA GAGACTATAA AAGAGGTCAT CTATGACATG
    CTGAATGCCC TGGCAGCATA TCACGCACCA GAAGAGGCAG AGAGAGGAGA GGAGCGGGCA
    AACGCTGTAC CAGGGAATCA AGACCTGTGT CAGATCCTGC AGGATGTCGG TGATGATGTC
    TACCAGCAGT ACAGACTCAC CAGACAAGGC AGTGATTTTG ACTCGCAATC TGCCTTCCCC
    ATCAATACTC AGGTGTTTGC TGCAGATGGT GCTGTAGCTG AATCTTCTCA GTCTGGAACT
    CCACAGGGAG AAGCCTCTAC ACCCGAGGAA ATGCGTGAAG AGAAAAAGGA GCAGGAAGGA
    GACAAGGGAG CTTCTTCGGA GGAGAGCCGA GCTGCTAAGG CTAAAGCTAG CAAACCTCTG
    ATGCCCACCT CTACCATCTT GAGGCTGTTG GCCGAGCTGG TTCGCTCTTA TGTTGGCATT
    GCCACTCTTA TCACCTCTTA CAACTACACA GCTGGCCAGT CGGAGCTCAT TAAGGAAGAT
    TGCAGTGTGT TGGCATTTGT TTTGGACCAC TTGCTCCCAC ACACCCAGAA CTCTGAAGAT
    AAGGACACCC CTGCCCTTGC ACGCCTTTTC TTGGCCAGCC TTGCTGCAGC TGGCACGGGC
    ACCGATGCCC AGGCTGCACT GGTCAACGAG GTCAAAGCTG CTTTGAGTCG AGCATTGGGC
    ATGGCAGAGG GCACAGAGAA ACACGCCCGT TTGCAAGCAG TGATGTGCAT CATCAGCACC
    ATTATGGAGT CGTGTCCTTC CACCTCCAGC TTCTACAGCA CAGCCACAGC TAAGACCCAG
    CACAACGGCA TGAACAATAT CATCCGGCTC TTCTTGAAGA AAGGCCTTGT CAATGACCTG
    GCCCGTGTGC CTCACAGCCT TGATCTATCC AGTCCCAATA TGGCAAACAC AGTAAATGCT
    GCTTTGAAGC CTCTGGAAAC ATTGTCGCGA ATTGTGAACC AGCCCAGCAG CCTGTTTGGT
    GGTAAAGGGG GATCCAGCAA GAACAAGACT GAACATGACA CTGTTGGCAC AGTCAGAGAC
    AGTAACAGCA ACACTCAGGA TCAAGGAGAA GTGGGTGAGG CAGAGCCGGT TGAGAATCGT
    GACCGAGGTC AAGCCACAGA CTCTGACCTG ATGGATGGAG AAGCCGAGGG AGACACTGTG
    GTCATTGCTG GACAGCCTGA GGTGCTGAGC ACTCAGGCAA TGCAGGTGGA GAACGAGTTG
    GTGGATTTGA TCGATGAACT TTTAGAGAGA GATGCCCAAA CTGTTAATAG TACCATCATC
    GTAGGTCGTT CTGGAGAGGA TGAGTCTCAA GAGGATGTGC TGATGGATGA AGCTCCTTCC
    AATATGAGTC AGGCCTCCAC TCTCCAAGCC AACAGAGAAG ACTCCATGAA CATCCTCGAG
    CCAGAAGATG AGGAGCACAC GCAGGAGGAA GACTCCAGTG GTAGCAACGA GGATGAGGAC
    AGTCAGGATG AAGAGGAAGA GGAGGAGGAA GAAGAGGAGG AAGATCAGGA TGATGAAGAG
    GGAGATGAGG ATGATGACGA TGAAGGCTCA GAGATGGAAC TGGATGAAGA TTTTCCAGAT
    ATCAATGCTG CCCCTCATAT TCGTTTTGAG CGATTCGACC GAGATGATGA CCTCATCATT
    GAGTTTGATA ATATGTTCTC CACTTCAGCT GACATCCCTC CCTCACCAGG AAATATCCCC
    AGCTCCCACC CTCTGATGGT GCGTCACGCT GACCATGGCT CGCTGACTCT TGGGGGATCG
    GGCACCAGTA GCAGATTGGC TCAAGGAATG GGACGTAGTC AACGTTCTCT CCGCCAGCTC
    ACGGCCAACA CAGGCCACAC GATCCATGTA CATTATCCTG GCAACCGTCA GCCTAACCCT
    CCACTCATTC TGCAGAGATT ATTGGGCCCC AGCGCTGCAG CTGATATCTT GCAGCTGTCC
    AGCTCTCTTC CTCTGCAGTC TCGAGGTCGT GCCCGTTTAA TGGTGGGTAA TGAAGATGTG
    CACATTATTG CCCGTTCAGA CGATGAGCTG CTGGATGACT TCTTCCATGA GCAGAGCAGC
    ACCGGAGGAC AAGCTGGCAC CCTTTCAAGT ATCCCGACTG CCTTAACCAG ATGGACAGAT
    GAGTGTAAAG TGCTGGATGC TGAGAGCATG CATGACTGTG TGGCAGTGGT GAAGGTGCCC
    ATACTGCAGC ACCTGGAGAG TTTGCGTGAT GAGGAGCTGG AGGAACGCAG GGAGAAAAGG
    AGGAGGCAGT TGGCAGAGGA AGAGGAGACT GCAAAGCAGA ATGAGAGTTC TGCAGCAGGG
    GAGGAGACCA GGGAACAGAG TCTGCAGGGC TCTGGGATTG GAACAGTCAA TGGAGCTGCA
    GAGAATGCTG CAGAAGGAGA ACAGGCCCAG AGTGGGGTGT CCTCCTGTCT GGACCCTCCT
    CGCACCTCTG AGGGTTTCCT CACAGCGCCC CCATCAGGAG AAGTCACTCC AACCACCCCT
    GCCCCCCATG AACAAGCCCT CGTGTCTCTG GAAACAGCCA TCAGTCAGCA GGTGCACCAG
    CCAATCACAG AGCTGCTGCT GGCCGACTCC AATCCTGACT CTCACGGAGC CTTGGCAGGG
    GCAGGGCTTC CCCAGCTCTC AGCACCTGAT AGGCCAAATT GTGAGGCAGA AGCATCTCAG
    ATGGAGATGT CACCTGCACC CACTATTGGT GAGAGAGGAG GAGGAGCATT GGATGCAGTC
    AGGGAAGCTT CACTCAGTCC AGATATAGCT GAAAGTTCAG AGCCTGTGCC AGTGGGTGTG
    TCTCAACTCG AGGGTTCGCC CATGGATACG AGCAGTCCCG CTTCAGGAAC GCAGGAAGAG
    CCGGCTGCCA GCTCAGTTCC ATCCAATCAC ATGTCTCAGG AGCTATCAGG AAGTGGTGAC
    AGCGGCCTCA CAGACAGGCA GACAGATGCA GAAACTGGAT CAACTTCTGT TTCATCTCCT
    GGCGAGACCA TGCCTCGGAG TGACAGTGCC GATAGCCAGT CGCAGGCCAT CCAGGAGGAG
    CCCCTCCCTT CCACCAGCAA TGAAGAGGAG GACCCTCTGG CAGGCATCAG TCTACCTGAA
    GGCGTGGATC CGTCTTTCTT GGCAGCACTG CCTGAAGACA TTCGTCGGGA GGTGCTGCAG
    AACCAACTGG GAATCAGACC TCCTTCTCGA CCTCCTGTCG CCTCCACTCT GACAACCACT
    GCTAGTGCTG TGCTAGGAGG AGGGCCAGGG GTGACCGAAG TCAGCCCTGA ATTCTTAGCG
    GCTCTCCCAC CTGCAATTCA GGAAGAGGTC CTGGCCCAGC AGCGGGCAGA GCAGCAGCGT
    CGGGAGTTAT CCCAGCAACC CACACAGGGT GATCAACCTC TGGACCCAGT CACATTCATC
    CAGACCCTGC CCTCTGAGCT GCGGCGCTCC GTTCTGGAAG ACATGGAGGA CAGTGTACTA
    GCGGTCATGC CTCCAGATAT TGCAGCAGAG GCTGCAGCAC TGCGAAGGGA ACAAGAGGCC
    AGACAGAGAC AACTGATGCA CGAGCGCTTG TTCGGTCACA GCTCCTCTTC AGCTCTGTCT
    GCCATCTTAC GCTCTCCCGC CTTCACCAGC CGCCTCGGGG GCAACCGTGG GGTACAGTAC
    ACCCGGCTGG CTGTGCAAAG AGGAGGGACG TTTCAAATGG GAGGGAGTGC CAATCATAGC
    AGACCCTCTA GTTCAAACGT TGACTCTCTC CTGCGCCTGC GGGGGCGTCT CTTGTTGGAC
    CACGAGGCCT TGTCCTGCCT TCTTGTGCTG CTTTTTGTGG ACGAGCCCAA ACTTAACACC
    AGCCGCCTCC ACCGAGTGCT GCGTAACTTG TGCTATCACT CTCAGACGCG TGGCTGGGTC
    ATCCGCAGTC TCCTGTCCAT CCTGCAGCGC AGCAGTGAGA GCGAGCTGTG CCTGGAGACT
    GCACGCTTGG AGGAGTCCCG TGGCAAACGC TCACTAGGCC AGGGCTCATC TAGTGGAGGA
    AAGGGTTGTT CTTCATCCTC CCAGTCGGCT ATAGCCTCTT CGTCATCGTC CTCCTCGTCC
    TCTTCTTCTC TGGAGCTGCT GAACAGGGTG GAGTCACGGA GTTCCAGTCA GCTCTCCTGG
    CTGTCTGTAT CAATGGACGC AGCTCTTGGA TGCAGAACCA ACATCTTTCA GATCCAGCGA
    GTATCAGGCA GGAAACACGC AGAGAGGCAT TCAGCAGGAG GGAGTGTGAG CGCAGGAGGA
    CTCACAGGTG GAGTGTCAGG AACAGGAATG ACGTGTACAG GGGGAGGTGG GTCAACTGTT
    CACATCCATC CACAGGCTGC GCCGGTGGTC TGTCGACATG TACTGGACAC CCTCATCCAA
    TTGGCCAAGG TATTTCCAAG TCACTTCACT CAACAACGCT GTAAAGATCC CCTATCCTCC
    TCCTCCTCTG AGTTGGACAC TCGTCTTTGT GCCAGCTCAA CACCGACTAT GAGTGCCAGT
    TGTCGGTCAG GTGGCACCAG TCAGAGTAAC TCCTCTGCTA CCCCAAGTAG TCTCACTTCT
    ATCCACAATT CCCTGAGCGC CTGCTCTGGT ACGCCACAGT CCCTAGGCAT CTCCACCGAC
    TTTTGGGATC TCCTGGTCAA GCTGGACAAC ATGAATGTGA GCCGAAAGGG GAAGGCCTCC
    ATGAAGACTG TCCCTCTAGG GGGTGTCGGC GAGACAGAGG GTGCGCTGTT CAGCCTTGAG
    GCGTCTCCGC TGGGCCAGCT GATGAATATG CTGTCCCACC CTGTGATCCG CCGTAGTTCC
    CTCCTCACGG AGAAACTGCT ACGTCTGCTG TCTCTGATCT CCATCGCTCT GCCTGACAAC
    AAGGCCACGG AGGTTCCAGC CAGCCATCCA GTGCCTCAGA CCCCAACTGC CACGCCATCT
    GCACCTGCTC CTCAAACCAC AGCAGGGTCA GGGACCATCT CAGCAGTGGC AGCTACAGGC
    ACTACGCAGG GTTCAGTCGG TTCAGTGGGT ACAACGCCAT CTCAACCAGC CGCTACAATA
    TCTGTGGTTG CAGGCAGCAC CACCGCCTCT AGCATTATTG CTGGAAAACT GAAGCCAGCA
    GTAAGCATCA CGGACATTGA ATCTAAACTG GCCTCTACTG GACTTACGGA GAAGCAGCTG
    CAGCTCTCTG TGGAGGTTCT GACATCTCAC TCGTGTTCAG AGGAAGGTCT GGAGGACGCA
    GCCAACATCT TGCTGCAGCT GTCCAGAGGG GACGCACCGA CCCGAGACAC TGTGCTTAGA
    CTTCTGCTCA GCGGAGCACG TCACCTTGGA TACACTCTCT GCAAACAAAT CGGCACTTTA
    TTAGCTGAGT TGAGGGAGTA TAATCTAGAG CAGCAGAGAA GAGCACGGGC TGATGCTCAG
    TCTCCTGAAG CTTCTCATGA TGACCCATCC ATCACCACAC GCCTCAAGGG CAAGATGACC
    AGCAGGTTTG ACGGGTCAGA GAGTGTGGTG ATTGTTGCTG CTCAGAAGCG CACGTTAGGT
    GGCCGGGAGC TTCAGTTGCC CTGCATGAGC TCGCTCACTT CCAAGACCTC AACGCAGAAG
    TTTTTCTTGC GGGTACTGCA GGTCATCATC CAGCTTAGAG AAGACACGCG GCGCGCTAAC
    AAGAAAGCCA AGCAGACAGG AGGAAGACTA AGTTCAACCA GTCTGGGCTC GGCCAGTAGT
    ATTCAGGCTG CGGTCCGTCA GCTGGAGGCT GAAGCGGACG CCATCATTCA GATGGTGAGA
    GAGGGACAGA GGGCACGCCG TCTACAGCAG GCCCCCCCAC CCTCTTCATC TGCATCCTCT
    TCCGCTGCTG CTGCTGCTGC ATCCGCTGCT GTGGCTGTGG CTGGATCGTC TGCCCCCAGT
    ACCTCTGCAA CCAACAACCC CCCCGCTGGC ACGGCCAGCG CAGCCACGTC GGAGGTTCAC
    ACTGTTGCCG AGCCACCAGC AGCTCAGAGA GATGATTCGC CCATGGATGT GGATCAGCCC
    TCTCCTTTGG AACAGGACCA GCCAACATTA GGTGAGGAGA GCAGCAGTCA GCAGGAGCAG
    GAGGAGCGTT TGCCTGACCT GCCGCTGTTA AGTGAGCAGC TGCTTTTGGA TGAGCTGTGG
    GACATGCTTG GAGAATGTCT GAAAGAGCTT GAAGAGTCTC ATGACCAACA TGCAGTTCTA
    GTGCTGCAGC CTGCAGTGGA GGCCTTTTTC TTGGTGCATG CAACAGAGCG GGAAAGCAAA
    CCACCAGTTC GGGACACTCG GGAGAGCCAG CTGTCACACA TCAAGGATGA GCCTCCACCC
    CTGTCACCCG CCCCTCTCAC ACCCGCTACC CCCTCCTCTC TGGACCCCTT TTTTTCCAGA
    GAGCCTTCCT CTATGCACAT CTCTTCCAAC CTGCCTCCAG ATACACAGAA GTTCCTCCGT
    TTCGCAGAGA CCCACCGCAC AGTGCTGAAT CAGATCCTGC GTCAATCCAC CACTCACCTG
    GCAGACGGGC CTTTCGCTGT TTTGGTTGAC TACATTCGCA TCTTGGACTT TGACGTCAAG
    CGCAAGTATT TCCGTCAGGA GCTGGAGCGT CTGGATGAAG GCTTGAGGAA AGAGGATATG
    GCCGTCCATG TGAGGAGAGA TCACGTCTTT GAAGATTCTT ACAGAGAGCT GCACCGCAAG
    AGCCCAGAAG ACATGAAGAA CAGATTGTAT ATAGTATTTG AAGGAGAGGA GGGTCAGGAT
    GCAGGCGGGC TGTTAAGAGA GTGGTACATG ATCATCTCCA GAGAAATGTT CAACCCCATG
    TATGCCCTGT TTCGCACCTC CCCCGGCGAT CGGGTCACCT ATACCATCAA CCCCTCCTCC
    CACTGCAACC CCAACCATCT CAGCTACTTC AAGTTTGTGG GACGTGTGGT GGCCAAAGCA
    GTCTACGACA ACAGGCTATT AGAGTGTTAC TTTACACGCA GCTTCTACAA GCACATCCTG
    GGCAAGAGCG TTAGGTATAC AGACATGGAA AGCGAAGACT ATCCCTTTTT CCAAGGTCTG
    GTTTATTTGT TGGAGAACGA TGTGTCGACT CTGGGCTACG AGCTGACATT CAGCACAGAG
    GTCCAAGAGT TTGGCGTGTG TGAAGTGAGA GATCTGAAGG CCAACGGTGC CAACATTCTT
    GTCACAGAAG AGAATAAGAA GGAATATGTG CATCTGGTCT GCCAGATGAA GATGACAGGT
    GCAATCCGTA AACAGCTGGC TGCCTTCCTG GAGGGCTTTT ATGAGATCAT CCCCAAGAGA
    CTGATCTCCA TCTTCACTGA GCAAGAGCTG GAGCTGCTCA TCTCCGGCCT GCCCACCATT
    GACATCGACG ACCTCAAGGC CAATACTGAA TACCATAAAT ATCAGTCCAG TTCTATTCAG
    ATCCAGTGGT TCTGGCGTGC TTTACGATCC TTCGACCAGG CTGACAGGGC CAAATTTCTG
    CAGTTTGTTA CGGGCACTTC CAAGGTGCCA CTGCAGGGCT TTGCTGCACT GGAGGGAATG
    AACGGCATTC AGAAGTTCCA GATCCACCGA GATGACCGCT CCACTGACCG CCTGCCCTCT
    GCGCACACTT GCTTTAACCA GTTGGACCTC CCAGCGTACG AGAGCTACGA GAAGTTAAGA
    CACATGCTGC TGCTGGCCAT TCAGGAATGT TCTGAAGGCT TCGGACTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329385.1
    CDS142..13755
    Translation

    Target ORF information:

    RefSeq Version XM_021473710.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473710.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAAGCAGCT
    CGCAGGCGTG AGGAGGAGGA GAGGAGAGCG AGGGAGCGGG TAGAGGAGGA AGAAGCTCGT
    TGTCTGGAGC GCTTTCTGGA AGCAGAGCCT CTTGACACCA CTGAGCTTCA TGCCTTCACA
    GACTCCATGC TGCCTGGCTG CTTCCACCTG CTCGATGAGC TGCCGGACAC AGTCTACCGC
    CTCTGTGATC TGCTCATGAC TGCTATTAAA AGAAACGGCC CTGAATACAG AGACCTCATC
    CTTAGACAAG TGGTCAATCA GGTGTGGGAG GCTGCTGACG TGTTGATAAA GGCTGCAGTG
    CCGCTAACCA CCAGCGACAC CAAGACAGTC TCTGAGTGGA CCAGACAGAT GGCGACCCTC
    CCTCAGGCCT CCAACCTGGC CACCCGCATC CTGCTGCTCA CGCTGCTCTT TGAGGAGCTG
    AAACTCTCCT GCGCTCGGGT GGTGGAGAGC AGTGGTGTCT TGACTGTGCT GATTAAGCTG
    TTGGAGGTGG TGCAGCCCTG TCTGCAAGCT GCCAAAGAGC AGAAGGACAT CCAAACGCCC
    AAATGGATCA CCCCTGTGCT CCTACTTATT GACTTTTATG AGAAAATGGC TGTTTCATCC
    AAACGAAGAG CTCAGATGAA CAAGTACCTC CAGCCCAATG GGAATAACTG GCGCTGGTTT
    GATGACCGTT CAGGCCGCTG GTGCAGCTAC AGTGCAAGTA ACAACAGCAC TATTGACTCT
    GCATGGAGGG CAGGAGAGAC CAGTGTCCGA TTTACAGCTG GCCGCAGAAG ATACACAGTT
    CAGTTCAACA CCATGGTGCA GGTAAATGAG GAGACTGGCA ACAGACGACC AGTTATGTTG
    ACGGTACAGC GGGTGCCACG CATTCCCAAG GCAACAAAAT CCGGAAGTGT TACTGACACG
    GAGAGAGAGG AGAGTGAACA GAGAGCAAAG ACTGAGGAAA CACAGACTGA CACAGATTCC
    AGCTCTGTGG CTGTAGAGAT GGCGCCCCCT AAAGAAGAAT CTGAGCTGAA GGATTCAACC
    CCAGCAGCTC CGTCAGTTCT CCAGGACCCC TCTGGTTCTA GTGGTATCGT TGTGCAGGGG
    CTGACAGAAG ACATGATCAC CATTTTAATC CGTGCCTGTG TCAGCATGAT CAGTGTTCCT
    GTGGACCCAG ACACTTTGCA CGCCACCTTG CGGCTCTGTC TGCGCCTCAC ACGCACACAC
    CATTACGCCA TGATGTTTGC AGAGCTCAAG AGCACCCGCA TGATTCTGAA TCTCACCCAG
    AGCTCAGGCT TTAATGGCTT CACCCCATTG GTCACCCTGC TCTTCCGCCA CATCATAGAG
    GACCCGGCCA CACTGCGGCA CACTATGGAG AAGGTGGTAC GTTCTGCAGT CACCAGTGGT
    GCAGGAAGCA CTACTTCAGG TGTGGTGTCA GGTAGTCTGG GCTCCAGGGA AATTAACTAC
    ATCCTGCGAG TCCTTGGCCC AGCGGCCTGT CGCAATCCAG AGTGCTTTAC GGAAACTGCC
    AAGAGTTGCA TCCGAATTGC TCTCCCGGCT CCAAGAGGTG CTGGCACTGC CTCAGATGAC
    GAGTTTGAGA ACTTCAGAAT TAAAGGTCCA AATGCTGTGC AACTCGTGAA GACCACCCCC
    CTAAAGCTCT CCCCGTTGCC CTCCATACCA GAGACTATAA AAGAGGTCAT CTATGACATG
    CTGAATGCCC TGGCAGCATA TCACGCACCA GAAGAGGCAG AGAGAGGAGA GGAGCGGGCA
    AACGCTGTAC CAGGGAATCA AGACCTGTGT CAGATCCTGC AGGATGTCGG TGATGATGTC
    TACCAGCAGT ACAGACTCAC CAGACAAGGC AGTGATTTTG ACTCGCAATC TGCCTTCCCC
    ATCAATACTC AGGTGTTTGC TGCAGATGGT GCTGTAGCTG AATCTTCTCA GTCTGGAACT
    CCACAGGGAG AAGCCTCTAC ACCCGAGGAA ATGCGTGAAG AGAAAAAGGA GCAGGAAGGA
    GACAAGGGAG CTTCTTCGGA GGAGAGCCGA GCTGCTAAGG CTAAAGCTAG CAAACCTCTG
    ATGCCCACCT CTACCATCTT GAGGCTGTTG GCCGAGCTGG TTCGCTCTTA TGTTGGCATT
    GCCACTCTTA TCACCTCTTA CAACTACACA GCTGGCCAGT CGGAGCTCAT TAAGGAAGAT
    TGCAGTGTGT TGGCATTTGT TTTGGACCAC TTGCTCCCAC ACACCCAGAA CTCTGAAGAT
    AAGGACACCC CTGCCCTTGC ACGCCTTTTC TTGGCCAGCC TTGCTGCAGC TGGCACGGGC
    ACCGATGCCC AGGCTGCACT GGTCAACGAG GTCAAAGCTG CTTTGAGTCG AGCATTGGGC
    ATGGCAGAGG GCACAGAGAA ACACGCCCGT TTGCAAGCAG TGATGTGCAT CATCAGCACC
    ATTATGGAGT CGTGTCCTTC CACCTCCAGC TTCTACAGCA CAGCCACAGC TAAGACCCAG
    CACAACGGCA TGAACAATAT CATCCGGCTC TTCTTGAAGA AAGGCCTTGT CAATGACCTG
    GCCCGTGTGC CTCACAGCCT TGATCTATCC AGTCCCAATA TGGCAAACAC AGTAAATGCT
    GCTTTGAAGC CTCTGGAAAC ATTGTCGCGA ATTGTGAACC AGCCCAGCAG CCTGTTTGGT
    GGTAAAGGGG GATCCAGCAA GAACAAGACT GAACATGACA CTGTTGGCAC AGTCAGAGAC
    AGTAACAGCA ACACTCAGGA TCAAGGAGAA GTGGGTGAGG CAGAGCCGGT TGAGAATCGT
    GACCGAGGTC AAGCCACAGA CTCTGACCTG ATGGATGGAG AAGCCGAGGG AGACACTGTG
    GTCATTGCTG GACAGCCTGA GGTGCTGAGC ACTCAGGCAA TGCAGGTGGA GAACGAGTTG
    GTGGATTTGA TCGATGAACT TTTAGAGAGA GATGCCCAAA CTGTTAATAG TACCATCATC
    GTAGGTCGTT CTGGAGAGGA TGAGTCTCAA GAGGATGTGC TGATGGATGA AGCTCCTTCC
    AATATGAGTC AGGCCTCCAC TCTCCAAGCC AACAGAGAAG ACTCCATGAA CATCCTCGAG
    CCAGAAGATG AGGAGCACAC GCAGGAGGAA GACTCCAGTG GTAGCAACGA GGATGAGGAC
    AGTCAGGATG AAGAGGAAGA GGAGGAGGAA GAAGAGGAGG AAGATCAGGA TGATGAAGAG
    GGAGATGAGG ATGATGACGA TGAAGGCTCA GAGATGGAAC TGGATGAAGA TTTTCCAGAT
    ATCAATGCTG CCCCTCATAT TCGTTTTGAG CGATTCGACC GAGATGATGA CCTCATCATT
    GAGTTTGATA ATATGTTCTC CACTTCAGCT GACATCCCTC CCTCACCAGG AAATATCCCC
    AGCTCCCACC CTCTGATGGT GCGTCACGCT GACCATGGCT CGCTGACTCT TGGGGGATCG
    GGCACCAGTA GCAGATTGGC TCAAGGAATG GGACGTAGTC AACGTTCTCT CCGCCAGCTC
    ACGGCCAACA CAGGCCACAC GATCCATGTA CATTATCCTG GCAACCGTCA GCCTAACCCT
    CCACTCATTC TGCAGAGATT ATTGGGCCCC AGCGCTGCAG CTGATATCTT GCAGCTGTCC
    AGCTCTCTTC CTCTGCAGTC TCGAGGTCGT GCCCGTTTAA TGGTGGGTAA TGAAGATGTG
    CACATTATTG CCCGTTCAGA CGATGAGCTG CTGGATGACT TCTTCCATGA GCAGAGCAGC
    ACCGGAGGAC AAGCTGGCAC CCTTTCAAGT ATCCCGACTG CCTTAACCAG ATGGACAGAT
    GAGTGTAAAG TGCTGGATGC TGAGAGCATG CATGACTGTG TGGCAGTGGT GAAGGTGCCC
    ATACTGCAGC ACCTGGAGAG TTTGCGTGAT GAGGAGCTGG AGGAACGCAG GGAGAAAAGG
    AGGAGGCAGT TGGCAGAGGA AGAGGAGACT GCAAAGCAGA ATGAGAGTTC TGCAGCAGGG
    GAGGAGACCA GGGAACAGAG TCTGCAGGGC TCTGGGATTG GAACAGTCAA TGGAGCTGCA
    GAGAATGCTG CAGAAGGAGA ACAGGCCCAG AGTGGGGTGT CCTCCTGTCT GGACCCTCCT
    CGCACCTCTG AGGGTTTCCT CACAGCGCCC CCATCAGGAG AAGTCACTCC AACCACCCCT
    GCCCCCCATG AACAAGCCCT CGTGTCTCTG GAAACAGCCA TCAGTCAGCA GGTGCACCAG
    CCAATCACAG AGCTGCTGCT GGCCGACTCC AATCCTGACT CTCACGGAGC CTTGGCAGGG
    GCAGGGCTTC CCCAGCTCTC AGCACCTGAT AGGCCAAATT GTGAGGCAGA AGCATCTCAG
    ATGGAGATGT CACCTGCACC CACTATTGGT GAGAGAGGAG GAGGAGCATT GGATGCAGTC
    AGGGAAGCTT CACTCAGTCC AGATATAGCT GAAAGTTCAG AGCCTGTGCC AGTGGGTGTG
    TCTCAACTCG AGGGTTCGCC CATGGATACG AGCAGTCCCG CTTCAGGAAC GCAGGAAGAG
    CCGGCTGCCA GCTCAGTTCC ATCCAATCAC ATGTCTCAGG AGCTATCAGG AAGTGGTGAC
    AGCGGCCTCA CAGACAGGCA GACAGATGCA GAAACTGGAT CAACTTCTGT TTCATCTCCT
    GGCGAGACCA TGCCTCGGAG TGACAGTGCC GATAGCCAGT CGCAGGCCAT CCAGGAGGAG
    CCCCTCCCTT CCACCAGCAA TGAAGAGGAG GACCCTCTGG CAGGCATCAG TCTACCTGAA
    GGCGTGGATC CGTCTTTCTT GGCAGCACTG CCTGAAGACA TTCGTCGGGA GGTGCTGCAG
    AACCAACTGG GAATCAGACC TCCTTCTCGA CCTCCTGTCG CCTCCACTCT GACAACCACT
    GCTAGTGCTG TGCTAGGAGG AGGGCCAGGG GTGACCGAAG TCAGCCCTGA ATTCTTAGCG
    GCTCTCCCAC CTGCAATTCA GGAAGAGGTC CTGGCCCAGC AGCGGGCAGA GCAGCAGCGT
    CGGGAGTTAT CCCAGCAACC CACACAGGGT GATCAACCTC TGGACCCAGT CACATTCATC
    CAGACCCTGC CCTCTGAGCT GCGGCGCTCC GTTCTGGAAG ACATGGAGGA CAGTGTACTA
    GCGGTCATGC CTCCAGATAT TGCAGCAGAG GCTGCAGCAC TGCGAAGGGA ACAAGAGGCC
    AGACAGAGAC AACTGATGCA CGAGCGCTTG TTCGGTCACA GCTCCTCTTC AGCTCTGTCT
    GCCATCTTAC GCTCTCCCGC CTTCACCAGC CGCCTCGGGG GCAACCGTGG GGTACAGTAC
    ACCCGGCTGG CTGTGCAAAG AGGAGGGACG TTTCAAATGG GAGGGAGTGC CAATCATAGC
    AGACCCTCTA GTTCAAACGT TGACTCTCTC CTGCGCCTGC GGGGGCGTCT CTTGTTGGAC
    CACGAGGCCT TGTCCTGCCT TCTTGTGCTG CTTTTTGTGG ACGAGCCCAA ACTTAACACC
    AGCCGCCTCC ACCGAGTGCT GCGTAACTTG TGCTATCACT CTCAGACGCG TGGCTGGGTC
    ATCCGCAGTC TCCTGTCCAT CCTGCAGCGC AGCAGTGAGA GCGAGCTGTG CCTGGAGACT
    GCACGCTTGG AGGAGTCCCG TGGCAAACGC TCACTAGGCC AGGGCTCATC TAGTGGAGGA
    AAGGGTTGTT CTTCATCCTC CCAGTCGGCT ATAGCCTCTT CGTCATCGTC CTCCTCGTCC
    TCTTCTTCTC TGGAGCTGCT GAACAGGGTG GAGTCACGGA GTTCCAGTCA GCTCTCCTGG
    CTGTCTGTAT CAATGGACGC AGCTCTTGGA TGCAGAACCA ACATCTTTCA GATCCAGCGA
    GTATCAGGCA GGAAACACGC AGAGAGGCAT TCAGCAGGAG GGAGTGTGAG CGCAGGAGGA
    CTCACAGGTG GAGTGTCAGG AACAGGAATG ACGTGTACAG GGGGAGGTGG GTCAACTGTT
    CACATCCATC CACAGGCTGC GCCGGTGGTC TGTCGACATG TACTGGACAC CCTCATCCAA
    TTGGCCAAGG TATTTCCAAG TCACTTCACT CAACAACGCT GTAAAGATCC CCTATCCTCC
    TCCTCCTCTG AGTTGGACAC TCGTCTTTGT GCCAGCTCAA CACCGACTAT GAGTGCCAGT
    TGTCGGTCAG GTGGCACCAG TCAGAGTAAC TCCTCTGCTA CCCCAAGTAG TCTCACTTCT
    ATCCACAATT CCCTGAGCGC CTGCTCTGGT ACGCCACAGT CCCTAGGCAT CTCCACCGAC
    TTTTGGGATC TCCTGGTCAA GCTGGACAAC ATGAATGTGA GCCGAAAGGG GAAGGCCTCC
    ATGAAGACTG TCCCTCTAGG GGGTGTCGGC GAGACAGAGG GTGCGCTGTT CAGCCTTGAG
    GCGTCTCCGC TGGGCCAGCT GATGAATATG CTGTCCCACC CTGTGATCCG CCGTAGTTCC
    CTCCTCACGG AGAAACTGCT ACGTCTGCTG TCTCTGATCT CCATCGCTCT GCCTGACAAC
    AAGGCCACGG AGGTTCCAGC CAGCCATCCA GTGCCTCAGA CCCCAACTGC CACGCCATCT
    GCACCTGCTC CTCAAACCAC AGCAGGGTCA GGGACCATCT CAGCAGTGGC AGCTACAGGC
    ACTACGCAGG GTTCAGTCGG TTCAGTGGGT ACAACGCCAT CTCAACCAGC CGCTACAATA
    TCTGTGGTTG CAGGCAGCAC CACCGCCTCT AGCATTATTG CTGGAAAACT GAAGCCAGCA
    GTAAGCATCA CGGACATTGA ATCTAAACTG GCCTCTACTG GACTTACGGA GAAGCAGCTG
    CAGCTCTCTG TGGAGGTTCT GACATCTCAC TCGTGTTCAG AGGAAGGTCT GGAGGACGCA
    GCCAACATCT TGCTGCAGCT GTCCAGAGGG GACGCACCGA CCCGAGACAC TGTGCTTAGA
    CTTCTGCTCA GCGGAGCACG TCACCTTGGA TACACTCTCT GCAAACAAAT CGGCACTTTA
    TTAGCTGAGT TGAGGGAGTA TAATCTAGAG CAGCAGAGAA GAGCACGGGC TGATGCTCAG
    TCTCCTGAAG CTTCTCATGA TGACCCATCC ATCACCACAC GCCTCAAGGG CAAGATGACC
    AGCAGGTTTG ACGGGTCAGA GAGTGTGGTG ATTGTTGCTG CTCAGAAGCG CACGTTAGGT
    GGCCGGGAGC TTCAGTTGCC CTGCATGAGC TCGCTCACTT CCAAGACCTC AACGCAGAAG
    TTTTTCTTGC GGGTACTGCA GGTCATCATC CAGCTTAGAG AAGACACGCG GCGCGCTAAC
    AAGAAAGCCA AGCAGACAGG AGGAAGACTA AGTTCAACCA GTCTGGGCTC GGCCAGTAGT
    ATTCAGGCTG CGGTCCGTCA GCTGGAGGCT GAAGCGGACG CCATCATTCA GATGGTGAGA
    GAGGGACAGA GGGCACGCCG TCTACAGCAG GCCCCCCCAC CCTCTTCATC TGCATCCTCT
    TCCGCTGCTG CTGCTGCTGC ATCCGCTGCT GTGGCTGTGG CTGGATCGTC TGCCCCCAGT
    ACCTCTGCAA CCAACAACCC CCCCGCTGGC ACGGCCAGCG CAGCCACGTC GGAGGTTCAC
    ACTGTTGCCG AGCCACCAGC AGCTCAGAGA GATGATTCGC CCATGGATGT GGATCAGCCC
    TCTCCTTTGG AACAGGACCA GCCAACATTA GGTGAGGAGA GCAGCAGTCA GCAGGAGCAG
    GAGGAGCGTT TGCCTGACCT GCCGCTGTTA AGTGAGCAGC TGCTTTTGGA TGAGCTGTGG
    GACATGCTTG GAGAATGTCT GAAAGAGCTT GAAGAGTCTC ATGACCAACA TGCAGTTCTA
    GTGCTGCAGC CTGCAGTGGA GGCCTTTTTC TTGGTGCATG CAACAGAGCG GGAAAGCAAA
    CCACCAGTTC GGGACACTCG GGAGAGCCAG CTGTCACACA TCAAGGATGA GCCTCCACCC
    CTGTCACCCG CCCCTCTCAC ACCCGCTACC CCCTCCTCTC TGGACCCCTT TTTTTCCAGA
    GAGCCTTCCT CTATGCACAT CTCTTCCAAC CTGCCTCCAG ATACACAGAA GTTCCTCCGT
    TTCGCAGAGA CCCACCGCAC AGTGCTGAAT CAGATCCTGC GTCAATCCAC CACTCACCTG
    GCAGACGGGC CTTTCGCTGT TTTGGTTGAC TACATTCGCA TCTTGGACTT TGACGTCAAG
    CGCAAGTATT TCCGTCAGGA GCTGGAGCGT CTGGATGAAG GCTTGAGGAA AGAGGATATG
    GCCGTCCATG TGAGGAGAGA TCACGTCTTT GAAGATTCTT ACAGAGAGCT GCACCGCAAG
    AGCCCAGAAG ACATGAAGAA CAGATTGTAT ATAGTATTTG AAGGAGAGGA GGGTCAGGAT
    GCAGGCGGGC TGTTAAGAGA GTGGTACATG ATCATCTCCA GAGAAATGTT CAACCCCATG
    TATGCCCTGT TTCGCACCTC CCCCGGCGAT CGGGTCACCT ATACCATCAA CCCCTCCTCC
    CACTGCAACC CCAACCATCT CAGCTACTTC AAGTTTGTGG GACGTGTGGT GGCCAAAGCA
    GTCTACGACA ACAGGCTATT AGAGTGTTAC TTTACACGCA GCTTCTACAA GCACATCCTG
    GGCAAGAGCG TTAGGTATAC AGACATGGAA AGCGAAGACT ATCCCTTTTT CCAAGGTCTG
    GTTTATTTGT TGGAGAACGA TGTGTCGACT CTGGGCTACG AGCTGACATT CAGCACAGAG
    GTCCAAGAGT TTGGCGTGTG TGAAGTGAGA GATCTGAAGG CCAACGGTGC CAACATTCTT
    GTCACAGAAG AGAATAAGAA GGAATATGTG CATCTGGTCT GCCAGATGAA GATGACAGGT
    GCAATCCGTA AACAGCTGGC TGCCTTCCTG GAGGGCTTTT ATGAGATCAT CCCCAAGAGA
    CTGATCTCCA TCTTCACTGA GCAAGAGCTG GAGCTGCTCA TCTCCGGCCT GCCCACCATT
    GACATCGACG ACCTCAAGGC CAATACTGAA TACCATAAAT ATCAGTCCAG TTCTATTCAG
    ATCCAGTGGT TCTGGCGTGC TTTACGATCC TTCGACCAGG CTGACAGGGC CAAATTTCTG
    CAGTTTGTTA CGGGCACTTC CAAGGTGCCA CTGCAGGGCT TTGCTGCACT GGAGGGAATG
    AACGGCATTC AGAAGTTCCA GATCCACCGA GATGACCGCT CCACTGACCG CCTGCCCTCT
    GCGCACACTT GCTTTAACCA GTTGGACCTC CCAGCGTACG AGAGCTACGA GAAGTTAAGA
    CACATGCTGC TGCTGGCCAT TCAGGAATGT TCTGAAGGCT TCGGACTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27859
    Clone ID Related Accession (Same CDS sequence) XM_005162282.3 , XM_021473710.1 , XM_005162282.4
    Accession Version XM_005162282.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162282.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAAGCAGCT
    CGCAGGCGTG AGGAGGAGGA GAGGAGAGCG AGGGAGCGGG TAGAGGAGGA AGAAGCTCGT
    TGTCTGGAGC GCTTTCTGGA AGCAGAGCCT CTTGACACCA CTGAGCTTCA TGCCTTCACA
    GACTCCATGC TGCCTGGCTG CTTCCACCTG CTCGATGAGC TGCCGGACAC AGTCTACCGC
    CTCTGTGATC TGCTCATGAC TGCTATTAAA AGAAACGGCC CTGAATACAG AGACCTCATC
    CTTAGACAAG TGGTCAATCA GGTGTGGGAG GCTGCTGACG TGTTGATAAA GGCTGCAGTG
    CCGCTAACCA CCAGCGACAC CAAGACAGTC TCTGAGTGGA CCAGACAGAT GGCGACCCTC
    CCTCAGGCCT CCAACCTGGC CACCCGCATC CTGCTGCTCA CGCTGCTCTT TGAGGAGCTG
    AAACTCTCCT GCGCTCGGGT GGTGGAGAGC AGTGGTGTCT TGACTGTGCT GATTAAGCTG
    TTGGAGGTGG TGCAGCCCTG TCTGCAAGCT GCCAAAGAGC AGAAGGACAT CCAAACGCCC
    AAATGGATCA CCCCTGTGCT CCTACTTATT GACTTTTATG AGAAAATGGC TGTTTCATCC
    AAACGAAGAG CTCAGATGAA CAAGTACCTC CAGCCCAATG GGAATAACTG GCGCTGGTTT
    GATGACCGTT CAGGCCGCTG GTGCAGCTAC AGTGCAAGTA ACAACAGCAC TATTGACTCT
    GCATGGAGGG CAGGAGAGAC CAGTGTCCGA TTTACAGCTG GCCGCAGAAG ATACACAGTT
    CAGTTCAACA CCATGGTGCA GGTAAATGAG GAGACTGGCA ACAGACGACC AGTTATGTTG
    ACGGTACAGC GGGTGCCACG CATTCCCAAG GCAACAAAAT CCGGAAGTGT TACTGACACG
    GAGAGAGAGG AGAGTGAACA GAGAGCAAAG ACTGAGGAAA CACAGACTGA CACAGATTCC
    AGCTCTGTGG CTGTAGAGAT GGCGCCCCCT AAAGAAGAAT CTGAGCTGAA GGATTCAACC
    CCAGCAGCTC CGTCAGTTCT CCAGGACCCC TCTGGTTCTA GTGGTATCGT TGTGCAGGGG
    CTGACAGAAG ACATGATCAC CATTTTAATC CGTGCCTGTG TCAGCATGAT CAGTGTTCCT
    GTGGACCCAG ACACTTTGCA CGCCACCTTG CGGCTCTGTC TGCGCCTCAC ACGCACACAC
    CATTACGCCA TGATGTTTGC AGAGCTCAAG AGCACCCGCA TGATTCTGAA TCTCACCCAG
    AGCTCAGGCT TTAATGGCTT CACCCCATTG GTCACCCTGC TCTTCCGCCA CATCATAGAG
    GACCCGGCCA CACTGCGGCA CACTATGGAG AAGGTGGTAC GTTCTGCAGT CACCAGTGGT
    GCAGGAAGCA CTACTTCAGG TGTGGTGTCA GGTAGTCTGG GCTCCAGGGA AATTAACTAC
    ATCCTGCGAG TCCTTGGCCC AGCGGCCTGT CGCAATCCAG AGTGCTTTAC GGAAACTGCC
    AAGAGTTGCA TCCGAATTGC TCTCCCGGCT CCAAGAGGTG CTGGCACTGC CTCAGATGAC
    GAGTTTGAGA ACTTCAGAAT TAAAGGTCCA AATGCTGTGC AACTCGTGAA GACCACCCCC
    CTAAAGCTCT CCCCGTTGCC CTCCATACCA GAGACTATAA AAGAGGTCAT CTATGACATG
    CTGAATGCCC TGGCAGCATA TCACGCACCA GAAGAGGCAG AGAGAGGAGA GGAGCGGGCA
    AACGCTGTAC CAGGGAATCA AGACCTGTGT CAGATCCTGC AGGATGTCGG TGATGATGTC
    TACCAGCAGT ACAGACTCAC CAGACAAGGC AGTGATTTTG ACTCGCAATC TGCCTTCCCC
    ATCAATACTC AGGTGTTTGC TGCAGATGGT GCTGTAGCTG AATCTTCTCA GTCTGGAACT
    CCACAGGGAG AAGCCTCTAC ACCCGAGGAA ATGCGTGAAG AGAAAAAGGA GCAGGAAGGA
    GACAAGGGAG CTTCTTCGGA GGAGAGCCGA GCTGCTAAGG CTAAAGCTAG CAAACCTCTG
    ATGCCCACCT CTACCATCTT GAGGCTGTTG GCCGAGCTGG TTCGCTCTTA TGTTGGCATT
    GCCACTCTTA TCACCTCTTA CAACTACACA GCTGGCCAGT CGGAGCTCAT TAAGGAAGAT
    TGCAGTGTGT TGGCATTTGT TTTGGACCAC TTGCTCCCAC ACACCCAGAA CTCTGAAGAT
    AAGGACACCC CTGCCCTTGC ACGCCTTTTC TTGGCCAGCC TTGCTGCAGC TGGCACGGGC
    ACCGATGCCC AGGCTGCACT GGTCAACGAG GTCAAAGCTG CTTTGAGTCG AGCATTGGGC
    ATGGCAGAGG GCACAGAGAA ACACGCCCGT TTGCAAGCAG TGATGTGCAT CATCAGCACC
    ATTATGGAGT CGTGTCCTTC CACCTCCAGC TTCTACAGCA CAGCCACAGC TAAGACCCAG
    CACAACGGCA TGAACAATAT CATCCGGCTC TTCTTGAAGA AAGGCCTTGT CAATGACCTG
    GCCCGTGTGC CTCACAGCCT TGATCTATCC AGTCCCAATA TGGCAAACAC AGTAAATGCT
    GCTTTGAAGC CTCTGGAAAC ATTGTCGCGA ATTGTGAACC AGCCCAGCAG CCTGTTTGGT
    GGTAAAGGGG GATCCAGCAA GAACAAGACT GAACATGACA CTGTTGGCAC AGTCAGAGAC
    AGTAACAGCA ACACTCAGGA TCAAGGAGAA GTGGGTGAGG CAGAGCCGGT TGAGAATCGT
    GACCGAGGTC AAGCCACAGA CTCTGACCTG ATGGATGGAG AAGCCGAGGG AGACACTGTG
    GTCATTGCTG GACAGCCTGA GGTGCTGAGC ACTCAGGCAA TGCAGGTGGA GAACGAGTTG
    GTGGATTTGA TCGATGAACT TTTAGAGAGA GATGCCCAAA CTGTTAATAG TACCATCATC
    GTAGGTCGTT CTGGAGAGGA TGAGTCTCAA GAGGATGTGC TGATGGATGA AGCTCCTTCC
    AATATGAGTC AGGCCTCCAC TCTCCAAGCC AACAGAGAAG ACTCCATGAA CATCCTCGAG
    CCAGAAGATG AGGAGCACAC GCAGGAGGAA GACTCCAGTG GTAGCAACGA GGATGAGGAC
    AGTCAGGATG AAGAGGAAGA GGAGGAGGAA GAAGAGGAGG AAGATCAGGA TGATGAAGAG
    GGAGATGAGG ATGATGACGA TGAAGGCTCA GAGATGGAAC TGGATGAAGA TTTTCCAGAT
    ATCAATGCTG CCCCTCATAT TCGTTTTGAG CGATTCGACC GAGATGATGA CCTCATCATT
    GAGTTTGATA ATATGTTCTC CACTTCAGCT GACATCCCTC CCTCACCAGG AAATATCCCC
    AGCTCCCACC CTCTGATGGT GCGTCACGCT GACCATGGCT CGCTGACTCT TGGGGGATCG
    GGCACCAGTA GCAGATTGGC TCAAGGAATG GGACGTAGTC AACGTTCTCT CCGCCAGCTC
    ACGGCCAACA CAGGCCACAC GATCCATGTA CATTATCCTG GCAACCGTCA GCCTAACCCT
    CCACTCATTC TGCAGAGATT ATTGGGCCCC AGCGCTGCAG CTGATATCTT GCAGCTGTCC
    AGCTCTCTTC CTCTGCAGTC TCGAGGTCGT GCCCGTTTAA TGGTGGGTAA TGAAGATGTG
    CACATTATTG CCCGTTCAGA CGATGAGCTG CTGGATGACT TCTTCCATGA GCAGAGCAGC
    ACCGGAGGAC AAGCTGGCAC CCTTTCAAGT ATCCCGACTG CCTTAACCAG ATGGACAGAT
    GAGTGTAAAG TGCTGGATGC TGAGAGCATG CATGACTGTG TGGCAGTGGT GAAGGTGCCC
    ATACTGCAGC ACCTGGAGAG TTTGCGTGAT GAGGAGCTGG AGGAACGCAG GGAGAAAAGG
    AGGAGGCAGT TGGCAGAGGA AGAGGAGACT GCAAAGCAGA ATGAGAGTTC TGCAGCAGGG
    GAGGAGACCA GGGAACAGAG TCTGCAGGGC TCTGGGATTG GAACAGTCAA TGGAGCTGCA
    GAGAATGCTG CAGAAGGAGA ACAGGCCCAG AGTGGGGTGT CCTCCTGTCT GGACCCTCCT
    CGCACCTCTG AGGGTTTCCT CACAGCGCCC CCATCAGGAG AAGTCACTCC AACCACCCCT
    GCCCCCCATG AACAAGCCCT CGTGTCTCTG GAAACAGCCA TCAGTCAGCA GGTGCACCAG
    CCAATCACAG AGCTGCTGCT GGCCGACTCC AATCCTGACT CTCACGGAGC CTTGGCAGGG
    GCAGGGCTTC CCCAGCTCTC AGCACCTGAT AGGCCAAATT GTGAGGCAGA AGCATCTCAG
    ATGGAGATGT CACCTGCACC CACTATTGGT GAGAGAGGAG GAGGAGCATT GGATGCAGTC
    AGGGAAGCTT CACTCAGTCC AGATATAGCT GAAAGTTCAG AGCCTGTGCC AGTGGGTGTG
    TCTCAACTCG AGGGTTCGCC CATGGATACG AGCAGTCCCG CTTCAGGAAC GCAGGAAGAG
    CCGGCTGCCA GCTCAGTTCC ATCCAATCAC ATGTCTCAGG AGCTATCAGG AAGTGGTGAC
    AGCGGCCTCA CAGACAGGCA GACAGATGCA GAAACTGGAT CAACTTCTGT TTCATCTCCT
    GGCGAGACCA TGCCTCGGAG TGACAGTGCC GATAGCCAGT CGCAGGCCAT CCAGGAGGAG
    CCCCTCCCTT CCACCAGCAA TGAAGAGGAG GACCCTCTGG CAGGCATCAG TCTACCTGAA
    GGCGTGGATC CGTCTTTCTT GGCAGCACTG CCTGAAGACA TTCGTCGGGA GGTGCTGCAG
    AACCAACTGG GAATCAGACC TCCTTCTCGA CCTCCTGTCG CCTCCACTCT GACAACCACT
    GCTAGTGCTG TGCTAGGAGG AGGGCCAGGG GTGACCGAAG TCAGCCCTGA ATTCTTAGCG
    GCTCTCCCAC CTGCAATTCA GGAAGAGGTC CTGGCCCAGC AGCGGGCAGA GCAGCAGCGT
    CGGGAGTTAT CCCAGCAACC CACACAGGGT GATCAACCTC TGGACCCAGT CACATTCATC
    CAGACCCTGC CCTCTGAGCT GCGGCGCTCC GTTCTGGAAG ACATGGAGGA CAGTGTACTA
    GCGGTCATGC CTCCAGATAT TGCAGCAGAG GCTGCAGCAC TGCGAAGGGA ACAAGAGGCC
    AGACAGAGAC AACTGATGCA CGAGCGCTTG TTCGGTCACA GCTCCTCTTC AGCTCTGTCT
    GCCATCTTAC GCTCTCCCGC CTTCACCAGC CGCCTCGGGG GCAACCGTGG GGTACAGTAC
    ACCCGGCTGG CTGTGCAAAG AGGAGGGACG TTTCAAATGG GAGGGAGTGC CAATCATAGC
    AGACCCTCTA GTTCAAACGT TGACTCTCTC CTGCGCCTGC GGGGGCGTCT CTTGTTGGAC
    CACGAGGCCT TGTCCTGCCT TCTTGTGCTG CTTTTTGTGG ACGAGCCCAA ACTTAACACC
    AGCCGCCTCC ACCGAGTGCT GCGTAACTTG TGCTATCACT CTCAGACGCG TGGCTGGGTC
    ATCCGCAGTC TCCTGTCCAT CCTGCAGCGC AGCAGTGAGA GCGAGCTGTG CCTGGAGACT
    GCACGCTTGG AGGAGTCCCG TGGCAAACGC TCACTAGGCC AGGGCTCATC TAGTGGAGGA
    AAGGGTTGTT CTTCATCCTC CCAGTCGGCT ATAGCCTCTT CGTCATCGTC CTCCTCGTCC
    TCTTCTTCTC TGGAGCTGCT GAACAGGGTG GAGTCACGGA GTTCCAGTCA GCTCTCCTGG
    CTGTCTGTAT CAATGGACGC AGCTCTTGGA TGCAGAACCA ACATCTTTCA GATCCAGCGA
    GTATCAGGCA GGAAACACGC AGAGAGGCAT TCAGCAGGAG GGAGTGTGAG CGCAGGAGGA
    CTCACAGGTG GAGTGTCAGG AACAGGAATG ACGTGTACAG GGGGAGGTGG GTCAACTGTT
    CACATCCATC CACAGGCTGC GCCGGTGGTC TGTCGACATG TACTGGACAC CCTCATCCAA
    TTGGCCAAGG TATTTCCAAG TCACTTCACT CAACAACGCT GTAAAGATCC CCTATCCTCC
    TCCTCCTCTG AGTTGGACAC TCGTCTTTGT GCCAGCTCAA CACCGACTAT GAGTGCCAGT
    TGTCGGTCAG GTGGCACCAG TCAGAGTAAC TCCTCTGCTA CCCCAAGTAG TCTCACTTCT
    ATCCACAATT CCCTGAGCGC CTGCTCTGGT ACGCCACAGT CCCTAGGCAT CTCCACCGAC
    TTTTGGGATC TCCTGGTCAA GCTGGACAAC ATGAATGTGA GCCGAAAGGG GAAGGCCTCC
    ATGAAGACTG TCCCTCTAGG GGGTGTCGGC GAGACAGAGG GTGCGCTGTT CAGCCTTGAG
    GCGTCTCCGC TGGGCCAGCT GATGAATATG CTGTCCCACC CTGTGATCCG CCGTAGTTCC
    CTCCTCACGG AGAAACTGCT ACGTCTGCTG TCTCTGATCT CCATCGCTCT GCCTGACAAC
    AAGGCCACGG AGGTTCCAGC CAGCCATCCA GTGCCTCAGA CCCCAACTGC CACGCCATCT
    GCACCTGCTC CTCAAACCAC AGCAGGGTCA GGGACCATCT CAGCAGTGGC AGCTACAGGC
    ACTACGCAGG GTTCAGTCGG TTCAGTGGGT ACAACGCCAT CTCAACCAGC CGCTACAATA
    TCTGTGGTTG CAGGCAGCAC CACCGCCTCT AGCATTATTG CTGGAAAACT GAAGCCAGCA
    GTAAGCATCA CGGACATTGA ATCTAAACTG GCCTCTACTG GACTTACGGA GAAGCAGCTG
    CAGCTCTCTG TGGAGGTTCT GACATCTCAC TCGTGTTCAG AGGAAGGTCT GGAGGACGCA
    GCCAACATCT TGCTGCAGCT GTCCAGAGGG GACGCACCGA CCCGAGACAC TGTGCTTAGA
    CTTCTGCTCA GCGGAGCACG TCACCTTGGA TACACTCTCT GCAAACAAAT CGGCACTTTA
    TTAGCTGAGT TGAGGGAGTA TAATCTAGAG CAGCAGAGAA GAGCACGGGC TGATGCTCAG
    TCTCCTGAAG CTTCTCATGA TGACCCATCC ATCACCACAC GCCTCAAGGG CAAGATGACC
    AGCAGGTTTG ACGGGTCAGA GAGTGTGGTG ATTGTTGCTG CTCAGAAGCG CACGTTAGGT
    GGCCGGGAGC TTCAGTTGCC CTGCATGAGC TCGCTCACTT CCAAGACCTC AACGCAGAAG
    TTTTTCTTGC GGGTACTGCA GGTCATCATC CAGCTTAGAG AAGACACGCG GCGCGCTAAC
    AAGAAAGCCA AGCAGACAGG AGGAAGACTA AGTTCAACCA GTCTGGGCTC GGCCAGTAGT
    ATTCAGGCTG CGGTCCGTCA GCTGGAGGCT GAAGCGGACG CCATCATTCA GATGGTGAGA
    GAGGGACAGA GGGCACGCCG TCTACAGCAG GCCCCCCCAC CCTCTTCATC TGCATCCTCT
    TCCGCTGCTG CTGCTGCTGC ATCCGCTGCT GTGGCTGTGG CTGGATCGTC TGCCCCCAGT
    ACCTCTGCAA CCAACAACCC CCCCGCTGGC ACGGCCAGCG CAGCCACGTC GGAGGTTCAC
    ACTGTTGCCG AGCCACCAGC AGCTCAGAGA GATGATTCGC CCATGGATGT GGATCAGCCC
    TCTCCTTTGG AACAGGACCA GCCAACATTA GGTGAGGAGA GCAGCAGTCA GCAGGAGCAG
    GAGGAGCGTT TGCCTGACCT GCCGCTGTTA AGTGAGCAGC TGCTTTTGGA TGAGCTGTGG
    GACATGCTTG GAGAATGTCT GAAAGAGCTT GAAGAGTCTC ATGACCAACA TGCAGTTCTA
    GTGCTGCAGC CTGCAGTGGA GGCCTTTTTC TTGGTGCATG CAACAGAGCG GGAAAGCAAA
    CCACCAGTTC GGGACACTCG GGAGAGCCAG CTGTCACACA TCAAGGATGA GCCTCCACCC
    CTGTCACCCG CCCCTCTCAC ACCCGCTACC CCCTCCTCTC TGGACCCCTT TTTTTCCAGA
    GAGCCTTCCT CTATGCACAT CTCTTCCAAC CTGCCTCCAG ATACACAGAA GTTCCTCCGT
    TTCGCAGAGA CCCACCGCAC AGTGCTGAAT CAGATCCTGC GTCAATCCAC CACTCACCTG
    GCAGACGGGC CTTTCGCTGT TTTGGTTGAC TACATTCGCA TCTTGGACTT TGACGTCAAG
    CGCAAGTATT TCCGTCAGGA GCTGGAGCGT CTGGATGAAG GCTTGAGGAA AGAGGATATG
    GCCGTCCATG TGAGGAGAGA TCACGTCTTT GAAGATTCTT ACAGAGAGCT GCACCGCAAG
    AGCCCAGAAG ACATGAAGAA CAGATTGTAT ATAGTATTTG AAGGAGAGGA GGGTCAGGAT
    GCAGGCGGGC TGTTAAGAGA GTGGTACATG ATCATCTCCA GAGAAATGTT CAACCCCATG
    TATGCCCTGT TTCGCACCTC CCCCGGCGAT CGGGTCACCT ATACCATCAA CCCCTCCTCC
    CACTGCAACC CCAACCATCT CAGCTACTTC AAGTTTGTGG GACGTGTGGT GGCCAAAGCA
    GTCTACGACA ACAGGCTATT AGAGTGTTAC TTTACACGCA GCTTCTACAA GCACATCCTG
    GGCAAGAGCG TTAGGTATAC AGACATGGAA AGCGAAGACT ATCCCTTTTT CCAAGGTCTG
    GTTTATTTGT TGGAGAACGA TGTGTCGACT CTGGGCTACG AGCTGACATT CAGCACAGAG
    GTCCAAGAGT TTGGCGTGTG TGAAGTGAGA GATCTGAAGG CCAACGGTGC CAACATTCTT
    GTCACAGAAG AGAATAAGAA GGAATATGTG CATCTGGTCT GCCAGATGAA GATGACAGGT
    GCAATCCGTA AACAGCTGGC TGCCTTCCTG GAGGGCTTTT ATGAGATCAT CCCCAAGAGA
    CTGATCTCCA TCTTCACTGA GCAAGAGCTG GAGCTGCTCA TCTCCGGCCT GCCCACCATT
    GACATCGACG ACCTCAAGGC CAATACTGAA TACCATAAAT ATCAGTCCAG TTCTATTCAG
    ATCCAGTGGT TCTGGCGTGC TTTACGATCC TTCGACCAGG CTGACAGGGC CAAATTTCTG
    CAGTTTGTTA CGGGCACTTC CAAGGTGCCA CTGCAGGGCT TTGCTGCACT GGAGGGAATG
    AACGGCATTC AGAAGTTCCA GATCCACCGA GATGACCGCT CCACTGACCG CCTGCCCTCT
    GCGCACACTT GCTTTAACCA GTTGGACCTC CCAGCGTACG AGAGCTACGA GAAGTTAAGA
    CACATGCTGC TGCTGGCCAT TCAGGAATGT TCTGAAGGCT TCGGACTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162339.1
    CDS142..13755
    Translation

    Target ORF information:

    RefSeq Version XM_005162282.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162282.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAAGCAGCT
    CGCAGGCGTG AGGAGGAGGA GAGGAGAGCG AGGGAGCGGG TAGAGGAGGA AGAAGCTCGT
    TGTCTGGAGC GCTTTCTGGA AGCAGAGCCT CTTGACACCA CTGAGCTTCA TGCCTTCACA
    GACTCCATGC TGCCTGGCTG CTTCCACCTG CTCGATGAGC TGCCGGACAC AGTCTACCGC
    CTCTGTGATC TGCTCATGAC TGCTATTAAA AGAAACGGCC CTGAATACAG AGACCTCATC
    CTTAGACAAG TGGTCAATCA GGTGTGGGAG GCTGCTGACG TGTTGATAAA GGCTGCAGTG
    CCGCTAACCA CCAGCGACAC CAAGACAGTC TCTGAGTGGA CCAGACAGAT GGCGACCCTC
    CCTCAGGCCT CCAACCTGGC CACCCGCATC CTGCTGCTCA CGCTGCTCTT TGAGGAGCTG
    AAACTCTCCT GCGCTCGGGT GGTGGAGAGC AGTGGTGTCT TGACTGTGCT GATTAAGCTG
    TTGGAGGTGG TGCAGCCCTG TCTGCAAGCT GCCAAAGAGC AGAAGGACAT CCAAACGCCC
    AAATGGATCA CCCCTGTGCT CCTACTTATT GACTTTTATG AGAAAATGGC TGTTTCATCC
    AAACGAAGAG CTCAGATGAA CAAGTACCTC CAGCCCAATG GGAATAACTG GCGCTGGTTT
    GATGACCGTT CAGGCCGCTG GTGCAGCTAC AGTGCAAGTA ACAACAGCAC TATTGACTCT
    GCATGGAGGG CAGGAGAGAC CAGTGTCCGA TTTACAGCTG GCCGCAGAAG ATACACAGTT
    CAGTTCAACA CCATGGTGCA GGTAAATGAG GAGACTGGCA ACAGACGACC AGTTATGTTG
    ACGGTACAGC GGGTGCCACG CATTCCCAAG GCAACAAAAT CCGGAAGTGT TACTGACACG
    GAGAGAGAGG AGAGTGAACA GAGAGCAAAG ACTGAGGAAA CACAGACTGA CACAGATTCC
    AGCTCTGTGG CTGTAGAGAT GGCGCCCCCT AAAGAAGAAT CTGAGCTGAA GGATTCAACC
    CCAGCAGCTC CGTCAGTTCT CCAGGACCCC TCTGGTTCTA GTGGTATCGT TGTGCAGGGG
    CTGACAGAAG ACATGATCAC CATTTTAATC CGTGCCTGTG TCAGCATGAT CAGTGTTCCT
    GTGGACCCAG ACACTTTGCA CGCCACCTTG CGGCTCTGTC TGCGCCTCAC ACGCACACAC
    CATTACGCCA TGATGTTTGC AGAGCTCAAG AGCACCCGCA TGATTCTGAA TCTCACCCAG
    AGCTCAGGCT TTAATGGCTT CACCCCATTG GTCACCCTGC TCTTCCGCCA CATCATAGAG
    GACCCGGCCA CACTGCGGCA CACTATGGAG AAGGTGGTAC GTTCTGCAGT CACCAGTGGT
    GCAGGAAGCA CTACTTCAGG TGTGGTGTCA GGTAGTCTGG GCTCCAGGGA AATTAACTAC
    ATCCTGCGAG TCCTTGGCCC AGCGGCCTGT CGCAATCCAG AGTGCTTTAC GGAAACTGCC
    AAGAGTTGCA TCCGAATTGC TCTCCCGGCT CCAAGAGGTG CTGGCACTGC CTCAGATGAC
    GAGTTTGAGA ACTTCAGAAT TAAAGGTCCA AATGCTGTGC AACTCGTGAA GACCACCCCC
    CTAAAGCTCT CCCCGTTGCC CTCCATACCA GAGACTATAA AAGAGGTCAT CTATGACATG
    CTGAATGCCC TGGCAGCATA TCACGCACCA GAAGAGGCAG AGAGAGGAGA GGAGCGGGCA
    AACGCTGTAC CAGGGAATCA AGACCTGTGT CAGATCCTGC AGGATGTCGG TGATGATGTC
    TACCAGCAGT ACAGACTCAC CAGACAAGGC AGTGATTTTG ACTCGCAATC TGCCTTCCCC
    ATCAATACTC AGGTGTTTGC TGCAGATGGT GCTGTAGCTG AATCTTCTCA GTCTGGAACT
    CCACAGGGAG AAGCCTCTAC ACCCGAGGAA ATGCGTGAAG AGAAAAAGGA GCAGGAAGGA
    GACAAGGGAG CTTCTTCGGA GGAGAGCCGA GCTGCTAAGG CTAAAGCTAG CAAACCTCTG
    ATGCCCACCT CTACCATCTT GAGGCTGTTG GCCGAGCTGG TTCGCTCTTA TGTTGGCATT
    GCCACTCTTA TCACCTCTTA CAACTACACA GCTGGCCAGT CGGAGCTCAT TAAGGAAGAT
    TGCAGTGTGT TGGCATTTGT TTTGGACCAC TTGCTCCCAC ACACCCAGAA CTCTGAAGAT
    AAGGACACCC CTGCCCTTGC ACGCCTTTTC TTGGCCAGCC TTGCTGCAGC TGGCACGGGC
    ACCGATGCCC AGGCTGCACT GGTCAACGAG GTCAAAGCTG CTTTGAGTCG AGCATTGGGC
    ATGGCAGAGG GCACAGAGAA ACACGCCCGT TTGCAAGCAG TGATGTGCAT CATCAGCACC
    ATTATGGAGT CGTGTCCTTC CACCTCCAGC TTCTACAGCA CAGCCACAGC TAAGACCCAG
    CACAACGGCA TGAACAATAT CATCCGGCTC TTCTTGAAGA AAGGCCTTGT CAATGACCTG
    GCCCGTGTGC CTCACAGCCT TGATCTATCC AGTCCCAATA TGGCAAACAC AGTAAATGCT
    GCTTTGAAGC CTCTGGAAAC ATTGTCGCGA ATTGTGAACC AGCCCAGCAG CCTGTTTGGT
    GGTAAAGGGG GATCCAGCAA GAACAAGACT GAACATGACA CTGTTGGCAC AGTCAGAGAC
    AGTAACAGCA ACACTCAGGA TCAAGGAGAA GTGGGTGAGG CAGAGCCGGT TGAGAATCGT
    GACCGAGGTC AAGCCACAGA CTCTGACCTG ATGGATGGAG AAGCCGAGGG AGACACTGTG
    GTCATTGCTG GACAGCCTGA GGTGCTGAGC ACTCAGGCAA TGCAGGTGGA GAACGAGTTG
    GTGGATTTGA TCGATGAACT TTTAGAGAGA GATGCCCAAA CTGTTAATAG TACCATCATC
    GTAGGTCGTT CTGGAGAGGA TGAGTCTCAA GAGGATGTGC TGATGGATGA AGCTCCTTCC
    AATATGAGTC AGGCCTCCAC TCTCCAAGCC AACAGAGAAG ACTCCATGAA CATCCTCGAG
    CCAGAAGATG AGGAGCACAC GCAGGAGGAA GACTCCAGTG GTAGCAACGA GGATGAGGAC
    AGTCAGGATG AAGAGGAAGA GGAGGAGGAA GAAGAGGAGG AAGATCAGGA TGATGAAGAG
    GGAGATGAGG ATGATGACGA TGAAGGCTCA GAGATGGAAC TGGATGAAGA TTTTCCAGAT
    ATCAATGCTG CCCCTCATAT TCGTTTTGAG CGATTCGACC GAGATGATGA CCTCATCATT
    GAGTTTGATA ATATGTTCTC CACTTCAGCT GACATCCCTC CCTCACCAGG AAATATCCCC
    AGCTCCCACC CTCTGATGGT GCGTCACGCT GACCATGGCT CGCTGACTCT TGGGGGATCG
    GGCACCAGTA GCAGATTGGC TCAAGGAATG GGACGTAGTC AACGTTCTCT CCGCCAGCTC
    ACGGCCAACA CAGGCCACAC GATCCATGTA CATTATCCTG GCAACCGTCA GCCTAACCCT
    CCACTCATTC TGCAGAGATT ATTGGGCCCC AGCGCTGCAG CTGATATCTT GCAGCTGTCC
    AGCTCTCTTC CTCTGCAGTC TCGAGGTCGT GCCCGTTTAA TGGTGGGTAA TGAAGATGTG
    CACATTATTG CCCGTTCAGA CGATGAGCTG CTGGATGACT TCTTCCATGA GCAGAGCAGC
    ACCGGAGGAC AAGCTGGCAC CCTTTCAAGT ATCCCGACTG CCTTAACCAG ATGGACAGAT
    GAGTGTAAAG TGCTGGATGC TGAGAGCATG CATGACTGTG TGGCAGTGGT GAAGGTGCCC
    ATACTGCAGC ACCTGGAGAG TTTGCGTGAT GAGGAGCTGG AGGAACGCAG GGAGAAAAGG
    AGGAGGCAGT TGGCAGAGGA AGAGGAGACT GCAAAGCAGA ATGAGAGTTC TGCAGCAGGG
    GAGGAGACCA GGGAACAGAG TCTGCAGGGC TCTGGGATTG GAACAGTCAA TGGAGCTGCA
    GAGAATGCTG CAGAAGGAGA ACAGGCCCAG AGTGGGGTGT CCTCCTGTCT GGACCCTCCT
    CGCACCTCTG AGGGTTTCCT CACAGCGCCC CCATCAGGAG AAGTCACTCC AACCACCCCT
    GCCCCCCATG AACAAGCCCT CGTGTCTCTG GAAACAGCCA TCAGTCAGCA GGTGCACCAG
    CCAATCACAG AGCTGCTGCT GGCCGACTCC AATCCTGACT CTCACGGAGC CTTGGCAGGG
    GCAGGGCTTC CCCAGCTCTC AGCACCTGAT AGGCCAAATT GTGAGGCAGA AGCATCTCAG
    ATGGAGATGT CACCTGCACC CACTATTGGT GAGAGAGGAG GAGGAGCATT GGATGCAGTC
    AGGGAAGCTT CACTCAGTCC AGATATAGCT GAAAGTTCAG AGCCTGTGCC AGTGGGTGTG
    TCTCAACTCG AGGGTTCGCC CATGGATACG AGCAGTCCCG CTTCAGGAAC GCAGGAAGAG
    CCGGCTGCCA GCTCAGTTCC ATCCAATCAC ATGTCTCAGG AGCTATCAGG AAGTGGTGAC
    AGCGGCCTCA CAGACAGGCA GACAGATGCA GAAACTGGAT CAACTTCTGT TTCATCTCCT
    GGCGAGACCA TGCCTCGGAG TGACAGTGCC GATAGCCAGT CGCAGGCCAT CCAGGAGGAG
    CCCCTCCCTT CCACCAGCAA TGAAGAGGAG GACCCTCTGG CAGGCATCAG TCTACCTGAA
    GGCGTGGATC CGTCTTTCTT GGCAGCACTG CCTGAAGACA TTCGTCGGGA GGTGCTGCAG
    AACCAACTGG GAATCAGACC TCCTTCTCGA CCTCCTGTCG CCTCCACTCT GACAACCACT
    GCTAGTGCTG TGCTAGGAGG AGGGCCAGGG GTGACCGAAG TCAGCCCTGA ATTCTTAGCG
    GCTCTCCCAC CTGCAATTCA GGAAGAGGTC CTGGCCCAGC AGCGGGCAGA GCAGCAGCGT
    CGGGAGTTAT CCCAGCAACC CACACAGGGT GATCAACCTC TGGACCCAGT CACATTCATC
    CAGACCCTGC CCTCTGAGCT GCGGCGCTCC GTTCTGGAAG ACATGGAGGA CAGTGTACTA
    GCGGTCATGC CTCCAGATAT TGCAGCAGAG GCTGCAGCAC TGCGAAGGGA ACAAGAGGCC
    AGACAGAGAC AACTGATGCA CGAGCGCTTG TTCGGTCACA GCTCCTCTTC AGCTCTGTCT
    GCCATCTTAC GCTCTCCCGC CTTCACCAGC CGCCTCGGGG GCAACCGTGG GGTACAGTAC
    ACCCGGCTGG CTGTGCAAAG AGGAGGGACG TTTCAAATGG GAGGGAGTGC CAATCATAGC
    AGACCCTCTA GTTCAAACGT TGACTCTCTC CTGCGCCTGC GGGGGCGTCT CTTGTTGGAC
    CACGAGGCCT TGTCCTGCCT TCTTGTGCTG CTTTTTGTGG ACGAGCCCAA ACTTAACACC
    AGCCGCCTCC ACCGAGTGCT GCGTAACTTG TGCTATCACT CTCAGACGCG TGGCTGGGTC
    ATCCGCAGTC TCCTGTCCAT CCTGCAGCGC AGCAGTGAGA GCGAGCTGTG CCTGGAGACT
    GCACGCTTGG AGGAGTCCCG TGGCAAACGC TCACTAGGCC AGGGCTCATC TAGTGGAGGA
    AAGGGTTGTT CTTCATCCTC CCAGTCGGCT ATAGCCTCTT CGTCATCGTC CTCCTCGTCC
    TCTTCTTCTC TGGAGCTGCT GAACAGGGTG GAGTCACGGA GTTCCAGTCA GCTCTCCTGG
    CTGTCTGTAT CAATGGACGC AGCTCTTGGA TGCAGAACCA ACATCTTTCA GATCCAGCGA
    GTATCAGGCA GGAAACACGC AGAGAGGCAT TCAGCAGGAG GGAGTGTGAG CGCAGGAGGA
    CTCACAGGTG GAGTGTCAGG AACAGGAATG ACGTGTACAG GGGGAGGTGG GTCAACTGTT
    CACATCCATC CACAGGCTGC GCCGGTGGTC TGTCGACATG TACTGGACAC CCTCATCCAA
    TTGGCCAAGG TATTTCCAAG TCACTTCACT CAACAACGCT GTAAAGATCC CCTATCCTCC
    TCCTCCTCTG AGTTGGACAC TCGTCTTTGT GCCAGCTCAA CACCGACTAT GAGTGCCAGT
    TGTCGGTCAG GTGGCACCAG TCAGAGTAAC TCCTCTGCTA CCCCAAGTAG TCTCACTTCT
    ATCCACAATT CCCTGAGCGC CTGCTCTGGT ACGCCACAGT CCCTAGGCAT CTCCACCGAC
    TTTTGGGATC TCCTGGTCAA GCTGGACAAC ATGAATGTGA GCCGAAAGGG GAAGGCCTCC
    ATGAAGACTG TCCCTCTAGG GGGTGTCGGC GAGACAGAGG GTGCGCTGTT CAGCCTTGAG
    GCGTCTCCGC TGGGCCAGCT GATGAATATG CTGTCCCACC CTGTGATCCG CCGTAGTTCC
    CTCCTCACGG AGAAACTGCT ACGTCTGCTG TCTCTGATCT CCATCGCTCT GCCTGACAAC
    AAGGCCACGG AGGTTCCAGC CAGCCATCCA GTGCCTCAGA CCCCAACTGC CACGCCATCT
    GCACCTGCTC CTCAAACCAC AGCAGGGTCA GGGACCATCT CAGCAGTGGC AGCTACAGGC
    ACTACGCAGG GTTCAGTCGG TTCAGTGGGT ACAACGCCAT CTCAACCAGC CGCTACAATA
    TCTGTGGTTG CAGGCAGCAC CACCGCCTCT AGCATTATTG CTGGAAAACT GAAGCCAGCA
    GTAAGCATCA CGGACATTGA ATCTAAACTG GCCTCTACTG GACTTACGGA GAAGCAGCTG
    CAGCTCTCTG TGGAGGTTCT GACATCTCAC TCGTGTTCAG AGGAAGGTCT GGAGGACGCA
    GCCAACATCT TGCTGCAGCT GTCCAGAGGG GACGCACCGA CCCGAGACAC TGTGCTTAGA
    CTTCTGCTCA GCGGAGCACG TCACCTTGGA TACACTCTCT GCAAACAAAT CGGCACTTTA
    TTAGCTGAGT TGAGGGAGTA TAATCTAGAG CAGCAGAGAA GAGCACGGGC TGATGCTCAG
    TCTCCTGAAG CTTCTCATGA TGACCCATCC ATCACCACAC GCCTCAAGGG CAAGATGACC
    AGCAGGTTTG ACGGGTCAGA GAGTGTGGTG ATTGTTGCTG CTCAGAAGCG CACGTTAGGT
    GGCCGGGAGC TTCAGTTGCC CTGCATGAGC TCGCTCACTT CCAAGACCTC AACGCAGAAG
    TTTTTCTTGC GGGTACTGCA GGTCATCATC CAGCTTAGAG AAGACACGCG GCGCGCTAAC
    AAGAAAGCCA AGCAGACAGG AGGAAGACTA AGTTCAACCA GTCTGGGCTC GGCCAGTAGT
    ATTCAGGCTG CGGTCCGTCA GCTGGAGGCT GAAGCGGACG CCATCATTCA GATGGTGAGA
    GAGGGACAGA GGGCACGCCG TCTACAGCAG GCCCCCCCAC CCTCTTCATC TGCATCCTCT
    TCCGCTGCTG CTGCTGCTGC ATCCGCTGCT GTGGCTGTGG CTGGATCGTC TGCCCCCAGT
    ACCTCTGCAA CCAACAACCC CCCCGCTGGC ACGGCCAGCG CAGCCACGTC GGAGGTTCAC
    ACTGTTGCCG AGCCACCAGC AGCTCAGAGA GATGATTCGC CCATGGATGT GGATCAGCCC
    TCTCCTTTGG AACAGGACCA GCCAACATTA GGTGAGGAGA GCAGCAGTCA GCAGGAGCAG
    GAGGAGCGTT TGCCTGACCT GCCGCTGTTA AGTGAGCAGC TGCTTTTGGA TGAGCTGTGG
    GACATGCTTG GAGAATGTCT GAAAGAGCTT GAAGAGTCTC ATGACCAACA TGCAGTTCTA
    GTGCTGCAGC CTGCAGTGGA GGCCTTTTTC TTGGTGCATG CAACAGAGCG GGAAAGCAAA
    CCACCAGTTC GGGACACTCG GGAGAGCCAG CTGTCACACA TCAAGGATGA GCCTCCACCC
    CTGTCACCCG CCCCTCTCAC ACCCGCTACC CCCTCCTCTC TGGACCCCTT TTTTTCCAGA
    GAGCCTTCCT CTATGCACAT CTCTTCCAAC CTGCCTCCAG ATACACAGAA GTTCCTCCGT
    TTCGCAGAGA CCCACCGCAC AGTGCTGAAT CAGATCCTGC GTCAATCCAC CACTCACCTG
    GCAGACGGGC CTTTCGCTGT TTTGGTTGAC TACATTCGCA TCTTGGACTT TGACGTCAAG
    CGCAAGTATT TCCGTCAGGA GCTGGAGCGT CTGGATGAAG GCTTGAGGAA AGAGGATATG
    GCCGTCCATG TGAGGAGAGA TCACGTCTTT GAAGATTCTT ACAGAGAGCT GCACCGCAAG
    AGCCCAGAAG ACATGAAGAA CAGATTGTAT ATAGTATTTG AAGGAGAGGA GGGTCAGGAT
    GCAGGCGGGC TGTTAAGAGA GTGGTACATG ATCATCTCCA GAGAAATGTT CAACCCCATG
    TATGCCCTGT TTCGCACCTC CCCCGGCGAT CGGGTCACCT ATACCATCAA CCCCTCCTCC
    CACTGCAACC CCAACCATCT CAGCTACTTC AAGTTTGTGG GACGTGTGGT GGCCAAAGCA
    GTCTACGACA ACAGGCTATT AGAGTGTTAC TTTACACGCA GCTTCTACAA GCACATCCTG
    GGCAAGAGCG TTAGGTATAC AGACATGGAA AGCGAAGACT ATCCCTTTTT CCAAGGTCTG
    GTTTATTTGT TGGAGAACGA TGTGTCGACT CTGGGCTACG AGCTGACATT CAGCACAGAG
    GTCCAAGAGT TTGGCGTGTG TGAAGTGAGA GATCTGAAGG CCAACGGTGC CAACATTCTT
    GTCACAGAAG AGAATAAGAA GGAATATGTG CATCTGGTCT GCCAGATGAA GATGACAGGT
    GCAATCCGTA AACAGCTGGC TGCCTTCCTG GAGGGCTTTT ATGAGATCAT CCCCAAGAGA
    CTGATCTCCA TCTTCACTGA GCAAGAGCTG GAGCTGCTCA TCTCCGGCCT GCCCACCATT
    GACATCGACG ACCTCAAGGC CAATACTGAA TACCATAAAT ATCAGTCCAG TTCTATTCAG
    ATCCAGTGGT TCTGGCGTGC TTTACGATCC TTCGACCAGG CTGACAGGGC CAAATTTCTG
    CAGTTTGTTA CGGGCACTTC CAAGGTGCCA CTGCAGGGCT TTGCTGCACT GGAGGGAATG
    AACGGCATTC AGAAGTTCCA GATCCACCGA GATGACCGCT CCACTGACCG CCTGCCCTCT
    GCGCACACTT GCTTTAACCA GTTGGACCTC CCAGCGTACG AGAGCTACGA GAAGTTAAGA
    CACATGCTGC TGCTGGCCAT TCAGGAATGT TCTGAAGGCT TCGGACTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43094
    Clone ID Related Accession (Same CDS sequence) XM_009296963.2 , XM_009296963.3 , XM_021473711.1 , NM_001365655.1
    Accession Version XM_009296963.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296963.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295238.1
    CDS149..13684
    Translation

    Target ORF information:

    RefSeq Version XM_009296963.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296963.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43094
    Clone ID Related Accession (Same CDS sequence) XM_009296963.2 , XM_009296963.3 , XM_021473711.1 , NM_001365655.1
    Accession Version XM_009296963.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296963.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1621
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    1801
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    1921
    1981
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    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295238.1
    CDS142..13677
    Translation

    Target ORF information:

    RefSeq Version XM_009296963.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296963.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43094
    Clone ID Related Accession (Same CDS sequence) XM_009296963.2 , XM_009296963.3 , XM_021473711.1 , NM_001365655.1
    Accession Version XM_021473711.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1261
    1321
    1381
    1441
    1501
    1561
    1621
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    1801
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    1981
    2041
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    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329386.1
    CDS142..13677
    Translation

    Target ORF information:

    RefSeq Version XM_021473711.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473711.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.