Huwe1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Huwe1
Entrez Gene ID	59026
Full Name	HECT, UBA and WWE domain containing 1
Synonyms	5430439H10Rik,AU041296,Arf-bp1,C430014N20Rik,C80292,Gm1718,Ib772,LASU1,Mule,Ureb1
General protein information	Preferred Names HECT, UBA and WWE domain containing 1 Names E3 ubiquitin-protein ligase HUWE1 E3Histone HECT, UBA and WWE domain-containing protein 1 HECT-type E3 ubiquitin transferase HUWE1 Mcl-1 ubiquitin ligase URE-B1 URE-binding protein 1 upstream regulatory element binding protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X F3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006528934.2	XP_006528997.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_006528936.2	XP_006528999.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_006528939.2	XP_006529002.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_011247852.1	XP_011246154.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_017318587.1	XP_017174076.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_011247853.2	XP_011246155.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_011247855.1	XP_011246157.1	E3 ubiquitin-protein ligase HUWE1 isoform X3
XM_006528934.3	XP_006528997.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_030251436.1	XP_030107296.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_006528938.3	XP_006529001.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_030251442.1	XP_030107302.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_030251444.1	XP_030107304.1	E3 ubiquitin-protein ligase HUWE1 isoform X3
XM_030251451.1	XP_030107311.1	E3 ubiquitin-protein ligase HUWE1 isoform X7
XM_006528951.3	XP_006529014.1	E3 ubiquitin-protein ligase HUWE1 isoform X11
XM_006528937.2	XP_006529000.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_006528938.2	XP_006529001.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_011247854.1	XP_011246156.1	E3 ubiquitin-protein ligase HUWE1 isoform X2
XM_011247858.1	XP_011246160.1	E3 ubiquitin-protein ligase HUWE1 isoform X7
XM_011247860.1	XP_011246162.1	E3 ubiquitin-protein ligase HUWE1 isoform X9
XM_030251435.1	XP_030107295.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_006528936.3	XP_006528999.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_030251437.1	XP_030107297.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_030251446.1	XP_030107306.1	E3 ubiquitin-protein ligase HUWE1 isoform X5
XM_030251447.1	XP_030107307.1	E3 ubiquitin-protein ligase HUWE1 isoform X6
XM_030251452.1	XP_030107312.1	E3 ubiquitin-protein ligase HUWE1 isoform X7
XM_006528949.4	XP_006529012.1	E3 ubiquitin-protein ligase HUWE1 isoform X8
XM_030251457.1	XP_030107317.1	E3 ubiquitin-protein ligase HUWE1 isoform X9
XM_006528940.2	XP_006529003.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_006528943.2	XP_006529006.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_011247859.1	XP_011246161.1	E3 ubiquitin-protein ligase HUWE1 isoform X8
XM_006528933.3	XP_006528996.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_006528937.3	XP_006529000.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_011247852.2	XP_011246154.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_030251439.1	XP_030107299.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_030251441.1	XP_030107301.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_030251448.1	XP_030107308.1	E3 ubiquitin-protein ligase HUWE1 isoform X7
XM_030251449.1	XP_030107309.1	E3 ubiquitin-protein ligase HUWE1 isoform X7
XM_030251455.1	XP_030107315.1	E3 ubiquitin-protein ligase HUWE1 isoform X8
XM_011247856.1	XP_011246158.1	E3 ubiquitin-protein ligase HUWE1 isoform X4
XM_011247857.1	XP_011246159.1	E3 ubiquitin-protein ligase HUWE1 isoform X5
XM_006528948.2	XP_006529011.1	E3 ubiquitin-protein ligase HUWE1 isoform X6
XM_006528951.2	XP_006529014.1	E3 ubiquitin-protein ligase HUWE1 isoform X10
XM_006528939.3	XP_006529002.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_006528943.3	XP_006529006.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_030251443.1	XP_030107303.1	E3 ubiquitin-protein ligase HUWE1 isoform X2
XM_030251445.1	XP_030107305.1	E3 ubiquitin-protein ligase HUWE1 isoform X4
XM_030251456.1	XP_030107316.1	E3 ubiquitin-protein ligase HUWE1 isoform X8
XM_030251458.1	XP_030107318.1	E3 ubiquitin-protein ligase HUWE1 isoform X10
XM_006528935.2	XP_006528998.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_006528933.2	XP_006528996.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_006528949.3	XP_006529012.1	E3 ubiquitin-protein ligase HUWE1 isoform X7
XM_030251434.1	XP_030107294.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_006528940.3	XP_006529003.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_030251438.1	XP_030107298.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_030251440.1	XP_030107300.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_006528948.3	XP_006529011.1	E3 ubiquitin-protein ligase HUWE1 isoform X7
XM_030251450.1	XP_030107310.1	E3 ubiquitin-protein ligase HUWE1 isoform X7
XM_030251453.1	XP_030107313.1	E3 ubiquitin-protein ligase HUWE1 isoform X7
XM_030251454.1	XP_030107314.1	E3 ubiquitin-protein ligase HUWE1 isoform X7
NM_021523.4	NP_067498.4	E3 ubiquitin-protein ligase HUWE1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	huwe1	XP_005162338.1
Drosophila melanogaster (fruit fly)	CG8184	NP_573059.1
Pan troglodytes (chimpanzee)	HUWE1	XP_003317522.2
Xenopus tropicalis (tropical clawed frog)	huwe1	XP_004914249.1
	HUWE1	XP_001088987.1
Mus musculus (house mouse)	Huwe1	NP_067498.4
Canis lupus familiaris (dog)	HUWE1	XP_005641438.1
Bos taurus (cattle)	HUWE1	NP_001103474.1
Homo sapiens (human)	HUWE1	NP_113584.3

Gene Ontology
Bibliography

Related articles in PubMed

Ubiquitin Ligase Huwe1 Modulates Spermatogenesis by Regulating Spermatogonial Differentiation and Entry into Meiosis.
Bose R, Sheng K, Moawad AR, Manku G, O'Flaherty C, Taketo T, Culty M, Fok KL, Wing SS
Scientific reports7(1)17759(2017 Dec)

Huwe1 Regulates the Establishment and Maintenance of Spermatogonia by Suppressing DNA Damage Response.
Fok KL, Bose R, Sheng K, Chang CW, Katz-Egorov M, Culty M, Su S, Yang M, Ruan YC, Chan HC, Iavarone A, Lasorella A, Cencic R, Pelletier J, Nagano M, Xu W, Wing SS
Endocrinology158(11)4000-4016(2017 Nov)

Huwe1 Sustains Normal Ovarian Epithelial Cell Transformation and Tumor Growth through the Histone H1.3-
Yang D, Sun B, Zhang X, Cheng D, Yu X, Yan L, Li L, An S, Jiang H, Lasorella A, Iavarone A, Zhang S, Zou F, Zhao X
Cancer research77(18)4773-4784(2017 Sep)

TGF?R signalling controls CD103
Bain CC, Montgomery J, Scott CL, Kel JM, Girard-Madoux MJH, Martens L, Zangerle-Murray TFP, Ober-Bl?baum J, Lindenbergh-Kortleve D, Samsom JN, Henri S, Lawrence T, Saeys Y, Malissen B, Dalod M, Clausen BE, Mowat AM
Nature communications8(1)620(2017 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A previously undefined role of Huwe1 in orchestrating the physiological DNA damage response in the male germline that contributes to the establishment and maintenance of spermatogonia.
Title: Huwe1 Regulates the Establishment and Maintenance of Spermatogonia by Suppressing DNA Damage Response.

Taken together, these data identify HUWE1 as a bona fide tumour suppressor gene in the intestinal epithelium and suggest a potential vulnerability of HUWE1-mutated tumours to DNA-damaging agents and inhibitors of anti-apoptotic proteins.
Title: HUWE1 is a critical colonic tumour suppressor gene that prevents MYC signalling, DNA damage accumulation and tumour initiation.

Mule also regulates protein levels of the receptor tyrosine kinase EphB3 by targeting it for proteasomal and lysosomal degradation.
Title: Mule Regulates the Intestinal Stem Cell Niche via the Wnt Pathway and Targets EphB3 for Proteasomal and Lysosomal Degradation.

this study shows that the E3-ubiquitin ligase Huwe1 (HECT, UBA, and WWE domain-containing 1) is required for proliferating stem cells of the adult mouse hippocampus to return to quiescence.
Title: Return to quiescence of mouse neural stem cells by degradation of a proactivation protein.

Thus, modulating the levels of both Huwe1 and USP10 appears to fine-tune the requisite degradation of TBP during myogenesis.
Title: A specific E3 ligase/deubiquitinase pair modulates TBP protein levels during muscle differentiation.

The following Huwe1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Huwe1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15194	NM_021523.4 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu15194	XM_006528949.4 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu15194	XM_030251456.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu15194	XM_011247858.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu15194	XM_030251455.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_030251435.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_030251438.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_011247852.2 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_030251434.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_030251439.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528936.3 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_011247853.2 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528943.3 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528937.3 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_030251440.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528940.3 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_017318587.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528938.3 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_030251437.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528939.3 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_030251436.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_030251442.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_030251441.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528935.2 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528934.3 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528933.3 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56719	XM_030251451.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56719	XM_030251450.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56719	XM_030251449.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56719	XM_030251454.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56719	XM_030251448.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56719	XM_030251453.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56719	XM_030251452.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56719	XM_006528948.3 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56721	XM_006528951.3 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu69810	XM_030251443.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu69810	XM_011247854.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu69811	XM_030251444.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu69811	XM_011247855.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu69812	XM_011247856.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu69812	XM_030251446.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu69813	XM_011247857.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu69813	XM_030251447.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu69814	XM_011247859.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu69815	XM_030251458.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu69815	XM_011247860.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu90433	XM_030251445.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu90434	XM_030251457.1 Latest version!	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OMu56714	XM_006528934.2	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528938.2	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528936.2	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528937.2	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_011247852.1	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56721	XM_006528951.2	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528939.2	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528943.2	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528933.2	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu15194	XM_006528949.3	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56714	XM_006528940.2	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu56719	XM_006528948.2	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Huwe1 ( NM_021523.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067498.4 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), mRNA.

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CloneID	OMu15194
Clone ID Related Accession (Same CDS sequence)	XM_011247858.1 , XM_006528949.4 , XM_030251455.1 , XM_030251456.1 , XM_006528949.3 , NM_021523.4
Accession Version	NM_021523.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB652132.1, AK165140.1, AY929611.1, DQ097265.1 and BC017642.1. On May 4, 2007 this sequence version replaced NM_021523.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ097265.1, AY772010.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGAGAAC AGGATGGAAC TGCTGGAAGC 
ATGGATGCAT CTGCCCAGGG TTTATTAGAA GGAATTGAAC TAGATGGTGA CACACTGGCA 
CCCATGGAGA CAGATGAACC TTCTTCTTCA GATTCTAAGG GCAAATCTAA AATCACACCT 
GCCATGGCTG CAAGAATTAA ACAAATCAAG CCGTTGCTGT CAGCTTCTTC CAGATTAGGC 
CGAGCACTTG CTGAGCTCTT TGGACTCCTT GTTAAGCTTT GTGTGGGTTC TCCTGTCCGC 
CAAAGGAGGA GCCATCATGC TGCCAGTACC ACCACTGCAC CAACACCTGC TGCTAGATCA 
ACAGCTTCAG CCCTCACTAA GCTACTCACA AAAGGCTTAT CATGGCAGCC CCCACCATAT 
ACACCCACTC CCCGCTTCAG GCTGACGTTC TTCATCTGTT CAGTTGGATT CACCTCTCCA 
ATGCTCTTTG ATGAGAGAAA GTATCCTTAC CACCTTATGC TGCAGAAATT TCTCTGCTCT 
GGTGGTCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TATCCATGGG AGGAAAAGTT 
CCTGTTTCTG AGGGATTGGA ACATTCAGAT CTACCTGATG GTACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTTGTAGA AAAGATGGTA AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGTTGCCTG CTAAATTGCC TGGAGGAGTC CAGAGCTTTC CACAATTTAG TGCCCTTCGC 
TTCCTTGTAG TAACTCAAAA AGCAGCCTTT ACTTGTATCA AGAACCTGTG GAACCGGAAA 
CCCCTTAAAG TATATGGGGG AAGAATGGCT GAGTCTATGC TGGCCATTTT GTGCCACATT 
CTCCGAGGGG AACCTGTGAT TCGAGAAAGA CTGAGCAAAG AGAAGGAAGG ATCTCGAGGA 
GAAGAAGAAG CAGGCCAGGA AGAAGGTGGC TCACGCAGAG AACCTCAAGT CAACCAGCAA 
CAGCTACAAC AGCTCATGGA CATGGGTTTC ACAAGAGAAC ATGCCATGGA GGCACTGTTG 
AATACCAGTA CGATGGAACA AGCAACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATA 
GGAGGAGTTG TTCGGGATCT TAGCATGTCA GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAAGA GCGTAAAGCT CGAGAAAAAC AAGAAGAGGA AGAAGCTAAA 
TGTCTAGAGA AGTTTCAAGA TGCTGACCCC TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACAATGT TACCAGGCTG CTTCCATCTT CTTGATGAGT TGCCTGATAC AGTGTACCGT 
GTTTGTGATC TGATCATGAC AGCTATCAAA CGTAATGGAG CTGACTATCG TGATATGATT 
CTAAAGCAAG TTGTCAACCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCTCTAACAA CAAGTGACAC AAAAACAGTT TCAGAGTGGA TCAGTCAGAT GGCTACCCTG 
CCACAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CACTACTTTT TGAGGAGTTG 
AAGTTACCTT GTGCCTGGGT GGTTGAATCA AGTGGCATTC TTAATGTCCT AATCAAACTC 
TTAGAAGTGG TTCAACCCTG CCTACAGGCT GCCAAAGAGC AAAAGGAAGT CCAGACCCCA 
AAGTGGATCA CCCCAGTGTT GCTCCTTATT GATTTTTATG AAAAGACAGC CATCTCATCA 
AAGAGAAGAG CCCAAATGAC TAAGTACCTA CAGTCCAACA GCAACAACTG GCGCTGGTTT 
GATGACCGCT CTGGGCGTTG GTGTAGTTAC AGTGCTAGCA ACAACAGCAC CATTGATTCT 
GCCTGGAAAT CTGGAGAAAC AAGTGTAAGA TTCACTGCTG GCCGACGAAG ATACACTGTC 
CAGTTCACTA CAATGGTGCA GGTTAATGAA GAAACAGGAA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTGCCACG ACTGAGTAAG AACTCAAAAA GCAGCAATGG ACAGGAACTA 
GAGAAGACAC TGGAAGAAAG CAAGGAGACC GATATCAAAC ATAAAGAAAA TAAAGGCAAT 
GATATCCCCT TAGCTCTAGA GAGCACAAAC ACTGAAAAAG AGGCAAGCCT GGATGAAACA 
AAAATTGGGG AGATCCTAAT CCAGGGCCTG ACAGAAGATA TGGTGACTGT TCTAATCCGT 
GCCTGTGTGA GCATGCTGGG AGTCCCTGTG GACCCAGACA CTTTGCATGC CACCCTTCGT 
CTCTGTTTAA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACACGAATGA TCTTGAATTT GACCCAGAGC TCAGGTTTCA ATGGGTTTAC CCCCTTAGTC 
ACCCTTCTCT TAAGGCACAT CATTGAGGAT CCTTGTACTC TTCGTCATAC TATGGAAAAG 
GTTGTTCGCT CGGCAGCCAC AAGTGGAGCT GGTAGTACTA CATCTGGTGT TGTATCTGGC 
AGCCTCGGTT CTCGGGAGAT CAACTATATC CTTAGAGTCC TAGGACCAGC TGCCTGCCGC 
AATCCAGACA TATTCACAGA AGTAGCCAAT TGCTGTATCC GCATTGCCCT TCCAGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGTCCTAAT 
GCTGTACAGC TGGTAAAGAC AACACCTTTG AAGCCCTCAT CTCTGCCTGT CATTCCTGAT 
ACTATTAAGG AAGTTATCTA TGATATGCTG AATGCTTTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGACA AATCTGATCC TAAACCTGGG GGAACGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGACGATGT ATACCAGCAG TACCGGTCAC TTACTCGTCA GAGCAGTGAC 
TTTGATACAC AGTCAGGTTT TTCCTTAAAT AGTCAGGTTT TTGCTGCAGA TGGTGCCCCT 
GCTGAAACTT CCACAACTGG AACCTCCCAA GGAGAGGGAG CTTCAACTCC AGAGGAGACT 
CGAGAAGGGA AGAAAGATAA AGAAGGGGAC CGTACATCTG AGGAAGGCAA GCAGAAAAGC 
AAGGGCAGCA AACCTTTAAT GCCTACCTCT ACTATCCTTC GTCTTCTGGC AGAGTTGGTG 
AGGTCCTATG TTGGTATTGC TACCCTGATT GCAAACTACA GCTATACCGT GGGCCAGTCT 
GAGCTGATCA AAGAGGACTG CAGTGTGCTA GCTTTTGTCC TGGACCACCT CCTCCCACAC 
ACCCAGAATG CAGAAGACAA GGATACACCT GCCCTGGCAC GTCTATTCCT TGCAAGTCTG 
GCTGCTGCGG GGAGTGGCAC AGATGCTCAA GTGGCCCTAG TGAATGAAGT GAAAGCAGCC 
CTGGGACGGG CGCTTGCTAT GGCTGAGAGT ACAGAGAAGC ACGCCAGACT TCAGGCAGTG 
ATGTGTATCA TCAGTACTAT CATGGAATCC TGCCCCTCCA CCTCCAGCTT CTACAGTAGT 
GCTACAGCCA AGACTCAGCA TAATGGCATG AACAACATCA TTCGACTGTT CCTAAAGAAG 
GGACTAGTCA ATGACCTGGC CAGAGTTCCT CACAGCCTAG ACCTGTCCAG TCCCAATATG 
GCCAACACAG TCAATGCTGC TCTAAAACCC TTGGAAACAC TTTCCCGGAT TGTAAATCAA 
CCCAGTAGCC TTTTTGGCAG CAAGAGTGCT TCTAGCAAGA ACAAGTCTGA GCAGGATGCT 
CAAGGAGCTT CTCAGGACTC CAGCAGCCAT CAGCAAGATC CAGGCGAGCC TGGGGAGGCA 
GAAGTGCAGG AGGAGGATCA TGATGTCACT CAGACAGAGG TGGCAGATGG GGATATCATG 
GATGGGGAGG CTGAAACCGA CTCAGTGGTG ATTGCCGGGC AGCCTGAGGT GCTCAGTTCA 
CAAGAGATGC AGGTTGAAAA TGAACTGGAG GACCTGATAG ATGAGTTGCT TGAGAGGGAT 
GGCGGATCTG GGAACAGTAC AATTATAGTG AGCAGAAGTG GAGAGGATGA ATCACAAGAG 
GACGTGCTGA TGGATGAAGC TCCTTCCAAC CTCAGCCAAG CTTCCACCTT GCAGGCCAAC 
CGAGAAGATT CCATGAATAT CCTGGACCCT GAGGATGAGG AGGAACATAC TCAGGAAGAG 
GACAGCAGTG GCAGTAACGA GGATGAGGAT GATAGTCAGG ATGAAGAGGA GGAGGAGGAG 
GAAGATGAGG AAGATGATCA GGAGGATGAT GAAGGTGAAG AAGGAGATGA AGATGATGAC 
GACGATGGTT CTGAGATGGA ATTGGATGAG GATTACCCTG ATATGAACGC TTCTCCCTTG 
GTCCGATTTG AGCGCTTTGA CCGGGAGGAT GATCTCATCA TTGAGTTTGA CAACATGTTC 
TCCAGTGCTA CAGACATTCC CCCATCCCCA GGAAATATCC CTACCACCCA CCCATTGATG 
GTACGTCATG CAGATCACAG TTCCCTGACA CTGGGCAGTG GTTCTTCCAC AACTCGTCTT 
ACCCAGGGCA TCGGTCGCAG TCAAAGGACC CTCAGACAGC TCACTGCCAA TACAGGCCAT 
ACCATTCATG TTCACTACCC TGGGAATCGC CAGCCTAACC CTCCTCTTAT ACTCCAGAGG 
TTGCTTGGTC CCTCTGCAGC TGCTGACATT CTTCAGCTGA GCAGCAGCCT TCCATTACAA 
AGCCGAGGCC GGGCCCGCCT CCTAGTGGGC AATGATGACG TCCATATCAT CGCCAGGTCT 
GATGACGAGC TGCTGGATGA CTTTTTTCAT GACCAGAGTA CAGCCACTAG CCAAGCAGGA 
ACCCTGTCCA GCATCCCAAC AGCTCTAACC CGATGGACAG AGGAATGCAA AGTTCTTGAT 
GCTGAGAGTA TGCATGACTG TGTTTCAGTG GTTAAAGTGC CCATTGTCAA TCACCTGGAA 
TTCTTGAGAG ATGAGGAGCT AGAAGAGAGA AGGGAGAAAC GCAGGAAACA ATTGGCTGAG 
GAAGAAACAA AAATAATAGA CAAAGGCAAA GAAGACAAGG AGAACAGAGA TCAGAGTGCA 
CAGTGTACTG TAACTAAGAC AAATGACTCC ACTGAACAGA ATGTCTCAGA TGGGACTCCT 
ATGCCTGACA GCTATCCAAC AACCCCCTCT TCAACGGATG CACCTACATC TGAGTCCAAG 
GAAACACTTG GTACCCTACA ACCCTCCCAG CAACAGCCAG CACTTCCACC CCCACCATCC 
TTAGGAGAGA TTCCTCAGGA ACTACAGTCC CCTGCTGAAG AAGTGGCAAA CTCTACACAG 
CTATTGATGC CTATAGAGTT GGAGGAATTG GGTCCCACAA GACCAAGTGG AGAAGCAGAA 
ACAACTCAAA TGGAATTGTC TCCAGCTCCC ACCATAACCT CTCTTTCCCC AGAGAGAGCT 
GAAGATTCAG ACGCGTTAAC AGCTGTCAGC AGTCAGCTGG AAGGTTCTCC CATGGACACC 
AGCAGCCTGG CTTCCTGTAC GTTAGAAGAG GCTGTGGGTG ACACTCCAGC AGCTGGCAGT 
TCTGAGCAGC CCACAGCAGG TAGTTCCACT CCTGGGGATG CGCCATCAGT TGTGGCAGAA 
GTGCAAGGCA GACCTGATGT ATCAAGAGAA TCCAACCAGC CACCTGAGGA CAGCTCCCCC 
CCTGCATCCT CGGAGAGTTC TTCCACTAGA GATTCTGCCG TGGCCATCTC TGGAGCAGAT 
TCTCGTGGAA TTCTAGAAGA GCCACTACCT TCAACAAGCA GTGAAGAAGA AGATCCCCTT 
GCTGGTATCA GTCTCCCTGA AGGTGTGGAT CCCTCCTTTC TGGCTGCTCT CCCTGATGAT 
ATCCGTCGGG AAGTCCTACA GAACCAGCTG GGCATACGTC CACCAACCCG AAGTGCTCCC 
TCCTCAAATA GCTCAGCTCC TGCAGTAGTG GGGAACCCTG GTGTAACTGA AGTGAGCCCT 
GAGTTCCTGG CTGCCCTACC TCCAGCTATT CAAGAGGAAG TGCTAGCACA GCAGAGAGCT 
GAACAGCAGC GACGGGAACT GGCTCAGAAT GCGAGCTCAG ACACCCCCAT GGACCCTGTG 
ACTTTCATTC AGACTTTGCC CTCAGATCTG CGCCGTAGTG TCCTAGAGGA TATGGAAGAC 
AGTGTGTTAG CTGTGATGCC ACCTGACATT GCAGCTGAGG CTCAAGCTCT AAGACGAGAA 
CAAGAAGCCC GACAGCGGCA GCTCATGCAT GAGCGTCTTT TTGGACACAG TAGTACTTCT 
GCACTCTCTG CCATTCTCCG AAGCCCGGCT TTTACCAGTC GCCTGAGTGG CAACCGTGGG 
GTCCAGTATA CTCGCCTTGC TGTACAGAGG GGTGGCACCT TCCAGATGGG GGGTAGCAGC 
AGCCATAATA GGCCTTCTGG CAGTAATGTG GACACACTAC TCCGCCTCCG AGGACGACTC 
CTTCTAGACC ATGAAGCCCT GTCTTGCCTC TTGGTCCTAC TTTTTGTGGA TGAGCCAAAG 
CTCAATACTA GCCGTCTACA CCGAGTACTG AGAAATCTCT GCTATCATGC CCAGACCCGC 
CACTGGGTCA TCCGCAGCCT CCTGTCCATT TTGCAGCGCA GCAGTGAAAG TGAGTTATGC 
ATTGAGACAC CTAAGCTCTC CACAAGTGAA GAAAGGGGCA AGAAATCAAG CAAGAGCTGT 
GCATCAAGCA GTCATGAGAA CCGTCCCCTG GATCTGCTCC ACAAGATGGA GTCCAAAAGT 
TCCAATCAGC TCTCCTGGCT CTCAGTATCA ATGGATGCTG CTTTAGGCTG TAGGACTAAC 
ATATTCCAGA TTCAACGTTC TGGAGGGCGT AAACATACAG AGAAGCATGC AAGCAGTGGT 
TCCACTGTCC ACATCCACCC CCAGGCTGCT CCTGTTGTTT GCAGACATGT TTTGGATACA 
CTCATTCAGT TGGCCAAGGT GTTTCCCAGC CACTTTACAC AGCAGCGGAC CAAAGAAACA 
AACTGTGAGA GTGATCGGGA ACGGGGCAGT AAGCAGGCCT GCAGCCCATG CTCCTCACAG 
TCCTCCAGCA GTGGCATCTG TACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG 
AATGTTAGCC GAAAAGGCAA GAACTCCGTG AAGTCAGTAC CAGTGAGCTC TGGTGGTGAA 
GGGGAAACTT CCCCACACAG CCTTGAAGCC TCTCCACTGG GGCAGCTCAT GAACATGTTG 
TCACATCCAG TCATCCGCCG GAGTTCTCTC TTAACTGAGA AACTCCTGAG ACTCCTCTCT 
CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTATCAGAAG TGCAAACTAA CTCCAGCAAC 
AGTGGTTCCT CCACTGCTGC CACCTCAAAC ACATCTACTA CTACCACCAC CACCACTACT 
GCCACTGCCC CCACGCCCAC ACCCCCAGCT GCAACTACCC CTGTCACTTC TGCTCCAGCT 
CTGGTTGCTG CCACAGCTAT TTCCACCATT ACTGTAGCTG CTTCTACCAC AGTGACTACC 
CCCACAACTG CTACCACTAC TGTTTCAACT TCTACTACTA AAGGCAGCAA ATCTCCAGCA 
AAGGTTGGTG AAGGAGGCAG TGGTATAGAC TTCAAAATGG TGTCCTCTGG CCTCACTGAA 
AACCAGCTAC AGCTCTCTGT AGAGGTGTTG ACATCCCACT CTTGTTCTGA AGAAGGCTTG 
GAAGATGCGG CCAATGTGTT ACTGCAGCTC TCTCGTGGGG ACTCTGGTAC CCGGGACACT 
GTTCTTAAGC TGCTACTAAA TGGAGCCCGC CATCTGGGTT ATACCCTCTG TAAACAGATA 
GGTACCCTAC TCGCTGAACT ACGGGAATAT AATCTGGAGC AGCAGCGGCG AGCCCAATGC 
GAGACCCTCT CTCCTGATGG CCTACCTGAG GAGCAACCAC AGACCACCAA ACTGAAAGGC 
AAAATGCAGA GCAGGTTTGA CATGGCTGAA AATGTGGTAA TTGTGGCATC TCAGAAGCGA 
CCCTTGGGTG GCCGGGAGCT CCAGCTGCCT TCTATGTCCA TGTTGACATC CAAGACATCT 
ACCCAGAAGT TCTTCTTGAG GGTACTACAG GTCATCATCC AGCTGCGGGA TGACACACGA 
CGAGCTAACA AGAAAGCCAA GCAGACAGGC AGGCTAGGTT CCTCCGGTTT AGGCTCAGCT 
AGCAGCATCC AGGCAGCTGT TCGGCAGCTG GAGGCTGAGG CTGATGCCAT TATACAAATG 
GTACGTGAGG GTCAAAGGGC GCGGAGACAG CAACAAGCAG CAACGTCGGA GTCTTCTAAC 
CAGTCAGAGA CATCTGTCCG GAGAGAGGAA TCGCCCATGG ATGTGGACCA GCCATCTCCT 
AGTGCTCAAG ATACTCAGTC CATTGTAATC TCAGATGGAA CACCACAGGG AGAGAAGGAG 
AAAGAGGAGA AACCACCTGA GTTACCCCTG CTCAGTGAGC AACTGAGCCT AGATGAACTG 
TGGGACATGC TAGGGGAATG TTTAAAAGAA CTGGAGGAAT CTCATGACCA GCATGCAGTA 
TTAGTGCTAC AGCCTGCTGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC 
AAGCCTCCTG TCCGAGATAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA TGAACCTCCT 
CCACTCTCCC CCGCCCCCTT AACCCCAGCC ACTCCATCCT CCCTTGACCC ATTCTTCTCC 
AGGGAGCCCT CATCTATGCA CATTTCCTCG AGCCTGCCCC CTGACACACA GAAGTTCCTT 
CGCTTTGCAG AGACTCACCG CACTGTGTTA AACCAGATAC TACGGCAGTC TACCACCCAT 
CTTGCTGATG GGCCTTTTGC TGTCTTGGTA GATTATATCC GTGTCCTCGA CTTTGATGTC 
AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG CGTTTGGACG AAGGGCTCCG GAAAGAAGAT 
ATGGCAGTGC ATGTCCGCCG TGACCATGTA TTTGAAGACT CCTATCGTGA GCTGCATCGC 
AAATCTCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG 
GATGCTGGGG GGCTCCTGCG AGAGTGGTAC ATGATCATCT CTCGAGAGAT GTTCAACCCT 
ATGTATGCCT TGTTCCGTAC CTCACCTGGT GACCGGGTCA CCTACACCAT CAATCCATCT 
TCCCACTGTA ACCCCAACCA CCTCAGCTAC TTCAAGTTTG TTGGACGAAT TGTAGCCAAA 
GCTGTATATG ACAACCGCCT CCTGGAGTGC TACTTTACTA GGTCTTTCTA CAAACACATC 
TTGGGCAAAT CTGTCAGGTA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC 
CTAGTTTATC TGCTTGAAAA TGATGTCTCT ACATTAGGCT ATGACCTCAC CTTCAGCACT 
GAGGTTCAAG AATTTGGAGT ATGTGAAGTT CGTGACCTCA AACCCAATGG AGCCAACATC 
TTGGTAACAG AGGAGAACAA GAAGGAATAT GTACACCTGG TTTGCCAGAT GAGAATGACA 
GGAGCCATCC GCAAACAGCT GGCAGCCTTC TTGGAGGGCT TCTATGAAAT CATTCCAAAG 
CGCCTCATAT CCATCTTTAC TGAGCAGGAA TTAGAGCTGC TCATATCAGG GCTGCCTACC 
ATCGACATTG ATGATCTAAA ATCTAACACT GAGTACCACA AGTACCAATC CAACTCTATT 
CAGATCCAGT GGTTTTGGAG AGCATTGCGT TCCTTTGACC AAGCAGACCG TGCCAAGTTC 
CTCCAGTTTG TCACAGGTAC TTCCAAGGTA CCCCTACAAG GATTTGCTGC CCTTGAAGGT 
ATGAATGGCA TTCAGAAGTT TCAGATTCAT CGAGATGACA GATCCACAGA TCGCCTGCCT 
TCAGCTCACA CATGTTTTAA TCAACTGGAC CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC 
CGCCACATGC TACTATTGGC CATCCAGGAA TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067498.4
CDS	454..13590
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	NM_021523.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021523.4

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGAGAAC AGGATGGAAC TGCTGGAAGC 
ATGGATGCAT CTGCCCAGGG TTTATTAGAA GGAATTGAAC TAGATGGTGA CACACTGGCA 
CCCATGGAGA CAGATGAACC TTCTTCTTCA GATTCTAAGG GCAAATCTAA AATCACACCT 
GCCATGGCTG CAAGAATTAA ACAAATCAAG CCGTTGCTGT CAGCTTCTTC CAGATTAGGC 
CGAGCACTTG CTGAGCTCTT TGGACTCCTT GTTAAGCTTT GTGTGGGTTC TCCTGTCCGC 
CAAAGGAGGA GCCATCATGC TGCCAGTACC ACCACTGCAC CAACACCTGC TGCTAGATCA 
ACAGCTTCAG CCCTCACTAA GCTACTCACA AAAGGCTTAT CATGGCAGCC CCCACCATAT 
ACACCCACTC CCCGCTTCAG GCTGACGTTC TTCATCTGTT CAGTTGGATT CACCTCTCCA 
ATGCTCTTTG ATGAGAGAAA GTATCCTTAC CACCTTATGC TGCAGAAATT TCTCTGCTCT 
GGTGGTCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TATCCATGGG AGGAAAAGTT 
CCTGTTTCTG AGGGATTGGA ACATTCAGAT CTACCTGATG GTACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTTGTAGA AAAGATGGTA AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGTTGCCTG CTAAATTGCC TGGAGGAGTC CAGAGCTTTC CACAATTTAG TGCCCTTCGC 
TTCCTTGTAG TAACTCAAAA AGCAGCCTTT ACTTGTATCA AGAACCTGTG GAACCGGAAA 
CCCCTTAAAG TATATGGGGG AAGAATGGCT GAGTCTATGC TGGCCATTTT GTGCCACATT 
CTCCGAGGGG AACCTGTGAT TCGAGAAAGA CTGAGCAAAG AGAAGGAAGG ATCTCGAGGA 
GAAGAAGAAG CAGGCCAGGA AGAAGGTGGC TCACGCAGAG AACCTCAAGT CAACCAGCAA 
CAGCTACAAC AGCTCATGGA CATGGGTTTC ACAAGAGAAC ATGCCATGGA GGCACTGTTG 
AATACCAGTA CGATGGAACA AGCAACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATA 
GGAGGAGTTG TTCGGGATCT TAGCATGTCA GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAAGA GCGTAAAGCT CGAGAAAAAC AAGAAGAGGA AGAAGCTAAA 
TGTCTAGAGA AGTTTCAAGA TGCTGACCCC TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACAATGT TACCAGGCTG CTTCCATCTT CTTGATGAGT TGCCTGATAC AGTGTACCGT 
GTTTGTGATC TGATCATGAC AGCTATCAAA CGTAATGGAG CTGACTATCG TGATATGATT 
CTAAAGCAAG TTGTCAACCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCTCTAACAA CAAGTGACAC AAAAACAGTT TCAGAGTGGA TCAGTCAGAT GGCTACCCTG 
CCACAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CACTACTTTT TGAGGAGTTG 
AAGTTACCTT GTGCCTGGGT GGTTGAATCA AGTGGCATTC TTAATGTCCT AATCAAACTC 
TTAGAAGTGG TTCAACCCTG CCTACAGGCT GCCAAAGAGC AAAAGGAAGT CCAGACCCCA 
AAGTGGATCA CCCCAGTGTT GCTCCTTATT GATTTTTATG AAAAGACAGC CATCTCATCA 
AAGAGAAGAG CCCAAATGAC TAAGTACCTA CAGTCCAACA GCAACAACTG GCGCTGGTTT 
GATGACCGCT CTGGGCGTTG GTGTAGTTAC AGTGCTAGCA ACAACAGCAC CATTGATTCT 
GCCTGGAAAT CTGGAGAAAC AAGTGTAAGA TTCACTGCTG GCCGACGAAG ATACACTGTC 
CAGTTCACTA CAATGGTGCA GGTTAATGAA GAAACAGGAA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTGCCACG ACTGAGTAAG AACTCAAAAA GCAGCAATGG ACAGGAACTA 
GAGAAGACAC TGGAAGAAAG CAAGGAGACC GATATCAAAC ATAAAGAAAA TAAAGGCAAT 
GATATCCCCT TAGCTCTAGA GAGCACAAAC ACTGAAAAAG AGGCAAGCCT GGATGAAACA 
AAAATTGGGG AGATCCTAAT CCAGGGCCTG ACAGAAGATA TGGTGACTGT TCTAATCCGT 
GCCTGTGTGA GCATGCTGGG AGTCCCTGTG GACCCAGACA CTTTGCATGC CACCCTTCGT 
CTCTGTTTAA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACACGAATGA TCTTGAATTT GACCCAGAGC TCAGGTTTCA ATGGGTTTAC CCCCTTAGTC 
ACCCTTCTCT TAAGGCACAT CATTGAGGAT CCTTGTACTC TTCGTCATAC TATGGAAAAG 
GTTGTTCGCT CGGCAGCCAC AAGTGGAGCT GGTAGTACTA CATCTGGTGT TGTATCTGGC 
AGCCTCGGTT CTCGGGAGAT CAACTATATC CTTAGAGTCC TAGGACCAGC TGCCTGCCGC 
AATCCAGACA TATTCACAGA AGTAGCCAAT TGCTGTATCC GCATTGCCCT TCCAGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGTCCTAAT 
GCTGTACAGC TGGTAAAGAC AACACCTTTG AAGCCCTCAT CTCTGCCTGT CATTCCTGAT 
ACTATTAAGG AAGTTATCTA TGATATGCTG AATGCTTTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGACA AATCTGATCC TAAACCTGGG GGAACGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGACGATGT ATACCAGCAG TACCGGTCAC TTACTCGTCA GAGCAGTGAC 
TTTGATACAC AGTCAGGTTT TTCCTTAAAT AGTCAGGTTT TTGCTGCAGA TGGTGCCCCT 
GCTGAAACTT CCACAACTGG AACCTCCCAA GGAGAGGGAG CTTCAACTCC AGAGGAGACT 
CGAGAAGGGA AGAAAGATAA AGAAGGGGAC CGTACATCTG AGGAAGGCAA GCAGAAAAGC 
AAGGGCAGCA AACCTTTAAT GCCTACCTCT ACTATCCTTC GTCTTCTGGC AGAGTTGGTG 
AGGTCCTATG TTGGTATTGC TACCCTGATT GCAAACTACA GCTATACCGT GGGCCAGTCT 
GAGCTGATCA AAGAGGACTG CAGTGTGCTA GCTTTTGTCC TGGACCACCT CCTCCCACAC 
ACCCAGAATG CAGAAGACAA GGATACACCT GCCCTGGCAC GTCTATTCCT TGCAAGTCTG 
GCTGCTGCGG GGAGTGGCAC AGATGCTCAA GTGGCCCTAG TGAATGAAGT GAAAGCAGCC 
CTGGGACGGG CGCTTGCTAT GGCTGAGAGT ACAGAGAAGC ACGCCAGACT TCAGGCAGTG 
ATGTGTATCA TCAGTACTAT CATGGAATCC TGCCCCTCCA CCTCCAGCTT CTACAGTAGT 
GCTACAGCCA AGACTCAGCA TAATGGCATG AACAACATCA TTCGACTGTT CCTAAAGAAG 
GGACTAGTCA ATGACCTGGC CAGAGTTCCT CACAGCCTAG ACCTGTCCAG TCCCAATATG 
GCCAACACAG TCAATGCTGC TCTAAAACCC TTGGAAACAC TTTCCCGGAT TGTAAATCAA 
CCCAGTAGCC TTTTTGGCAG CAAGAGTGCT TCTAGCAAGA ACAAGTCTGA GCAGGATGCT 
CAAGGAGCTT CTCAGGACTC CAGCAGCCAT CAGCAAGATC CAGGCGAGCC TGGGGAGGCA 
GAAGTGCAGG AGGAGGATCA TGATGTCACT CAGACAGAGG TGGCAGATGG GGATATCATG 
GATGGGGAGG CTGAAACCGA CTCAGTGGTG ATTGCCGGGC AGCCTGAGGT GCTCAGTTCA 
CAAGAGATGC AGGTTGAAAA TGAACTGGAG GACCTGATAG ATGAGTTGCT TGAGAGGGAT 
GGCGGATCTG GGAACAGTAC AATTATAGTG AGCAGAAGTG GAGAGGATGA ATCACAAGAG 
GACGTGCTGA TGGATGAAGC TCCTTCCAAC CTCAGCCAAG CTTCCACCTT GCAGGCCAAC 
CGAGAAGATT CCATGAATAT CCTGGACCCT GAGGATGAGG AGGAACATAC TCAGGAAGAG 
GACAGCAGTG GCAGTAACGA GGATGAGGAT GATAGTCAGG ATGAAGAGGA GGAGGAGGAG 
GAAGATGAGG AAGATGATCA GGAGGATGAT GAAGGTGAAG AAGGAGATGA AGATGATGAC 
GACGATGGTT CTGAGATGGA ATTGGATGAG GATTACCCTG ATATGAACGC TTCTCCCTTG 
GTCCGATTTG AGCGCTTTGA CCGGGAGGAT GATCTCATCA TTGAGTTTGA CAACATGTTC 
TCCAGTGCTA CAGACATTCC CCCATCCCCA GGAAATATCC CTACCACCCA CCCATTGATG 
GTACGTCATG CAGATCACAG TTCCCTGACA CTGGGCAGTG GTTCTTCCAC AACTCGTCTT 
ACCCAGGGCA TCGGTCGCAG TCAAAGGACC CTCAGACAGC TCACTGCCAA TACAGGCCAT 
ACCATTCATG TTCACTACCC TGGGAATCGC CAGCCTAACC CTCCTCTTAT ACTCCAGAGG 
TTGCTTGGTC CCTCTGCAGC TGCTGACATT CTTCAGCTGA GCAGCAGCCT TCCATTACAA 
AGCCGAGGCC GGGCCCGCCT CCTAGTGGGC AATGATGACG TCCATATCAT CGCCAGGTCT 
GATGACGAGC TGCTGGATGA CTTTTTTCAT GACCAGAGTA CAGCCACTAG CCAAGCAGGA 
ACCCTGTCCA GCATCCCAAC AGCTCTAACC CGATGGACAG AGGAATGCAA AGTTCTTGAT 
GCTGAGAGTA TGCATGACTG TGTTTCAGTG GTTAAAGTGC CCATTGTCAA TCACCTGGAA 
TTCTTGAGAG ATGAGGAGCT AGAAGAGAGA AGGGAGAAAC GCAGGAAACA ATTGGCTGAG 
GAAGAAACAA AAATAATAGA CAAAGGCAAA GAAGACAAGG AGAACAGAGA TCAGAGTGCA 
CAGTGTACTG TAACTAAGAC AAATGACTCC ACTGAACAGA ATGTCTCAGA TGGGACTCCT 
ATGCCTGACA GCTATCCAAC AACCCCCTCT TCAACGGATG CACCTACATC TGAGTCCAAG 
GAAACACTTG GTACCCTACA ACCCTCCCAG CAACAGCCAG CACTTCCACC CCCACCATCC 
TTAGGAGAGA TTCCTCAGGA ACTACAGTCC CCTGCTGAAG AAGTGGCAAA CTCTACACAG 
CTATTGATGC CTATAGAGTT GGAGGAATTG GGTCCCACAA GACCAAGTGG AGAAGCAGAA 
ACAACTCAAA TGGAATTGTC TCCAGCTCCC ACCATAACCT CTCTTTCCCC AGAGAGAGCT 
GAAGATTCAG ACGCGTTAAC AGCTGTCAGC AGTCAGCTGG AAGGTTCTCC CATGGACACC 
AGCAGCCTGG CTTCCTGTAC GTTAGAAGAG GCTGTGGGTG ACACTCCAGC AGCTGGCAGT 
TCTGAGCAGC CCACAGCAGG TAGTTCCACT CCTGGGGATG CGCCATCAGT TGTGGCAGAA 
GTGCAAGGCA GACCTGATGT ATCAAGAGAA TCCAACCAGC CACCTGAGGA CAGCTCCCCC 
CCTGCATCCT CGGAGAGTTC TTCCACTAGA GATTCTGCCG TGGCCATCTC TGGAGCAGAT 
TCTCGTGGAA TTCTAGAAGA GCCACTACCT TCAACAAGCA GTGAAGAAGA AGATCCCCTT 
GCTGGTATCA GTCTCCCTGA AGGTGTGGAT CCCTCCTTTC TGGCTGCTCT CCCTGATGAT 
ATCCGTCGGG AAGTCCTACA GAACCAGCTG GGCATACGTC CACCAACCCG AAGTGCTCCC 
TCCTCAAATA GCTCAGCTCC TGCAGTAGTG GGGAACCCTG GTGTAACTGA AGTGAGCCCT 
GAGTTCCTGG CTGCCCTACC TCCAGCTATT CAAGAGGAAG TGCTAGCACA GCAGAGAGCT 
GAACAGCAGC GACGGGAACT GGCTCAGAAT GCGAGCTCAG ACACCCCCAT GGACCCTGTG 
ACTTTCATTC AGACTTTGCC CTCAGATCTG CGCCGTAGTG TCCTAGAGGA TATGGAAGAC 
AGTGTGTTAG CTGTGATGCC ACCTGACATT GCAGCTGAGG CTCAAGCTCT AAGACGAGAA 
CAAGAAGCCC GACAGCGGCA GCTCATGCAT GAGCGTCTTT TTGGACACAG TAGTACTTCT 
GCACTCTCTG CCATTCTCCG AAGCCCGGCT TTTACCAGTC GCCTGAGTGG CAACCGTGGG 
GTCCAGTATA CTCGCCTTGC TGTACAGAGG GGTGGCACCT TCCAGATGGG GGGTAGCAGC 
AGCCATAATA GGCCTTCTGG CAGTAATGTG GACACACTAC TCCGCCTCCG AGGACGACTC 
CTTCTAGACC ATGAAGCCCT GTCTTGCCTC TTGGTCCTAC TTTTTGTGGA TGAGCCAAAG 
CTCAATACTA GCCGTCTACA CCGAGTACTG AGAAATCTCT GCTATCATGC CCAGACCCGC 
CACTGGGTCA TCCGCAGCCT CCTGTCCATT TTGCAGCGCA GCAGTGAAAG TGAGTTATGC 
ATTGAGACAC CTAAGCTCTC CACAAGTGAA GAAAGGGGCA AGAAATCAAG CAAGAGCTGT 
GCATCAAGCA GTCATGAGAA CCGTCCCCTG GATCTGCTCC ACAAGATGGA GTCCAAAAGT 
TCCAATCAGC TCTCCTGGCT CTCAGTATCA ATGGATGCTG CTTTAGGCTG TAGGACTAAC 
ATATTCCAGA TTCAACGTTC TGGAGGGCGT AAACATACAG AGAAGCATGC AAGCAGTGGT 
TCCACTGTCC ACATCCACCC CCAGGCTGCT CCTGTTGTTT GCAGACATGT TTTGGATACA 
CTCATTCAGT TGGCCAAGGT GTTTCCCAGC CACTTTACAC AGCAGCGGAC CAAAGAAACA 
AACTGTGAGA GTGATCGGGA ACGGGGCAGT AAGCAGGCCT GCAGCCCATG CTCCTCACAG 
TCCTCCAGCA GTGGCATCTG TACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG 
AATGTTAGCC GAAAAGGCAA GAACTCCGTG AAGTCAGTAC CAGTGAGCTC TGGTGGTGAA 
GGGGAAACTT CCCCACACAG CCTTGAAGCC TCTCCACTGG GGCAGCTCAT GAACATGTTG 
TCACATCCAG TCATCCGCCG GAGTTCTCTC TTAACTGAGA AACTCCTGAG ACTCCTCTCT 
CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTATCAGAAG TGCAAACTAA CTCCAGCAAC 
AGTGGTTCCT CCACTGCTGC CACCTCAAAC ACATCTACTA CTACCACCAC CACCACTACT 
GCCACTGCCC CCACGCCCAC ACCCCCAGCT GCAACTACCC CTGTCACTTC TGCTCCAGCT 
CTGGTTGCTG CCACAGCTAT TTCCACCATT ACTGTAGCTG CTTCTACCAC AGTGACTACC 
CCCACAACTG CTACCACTAC TGTTTCAACT TCTACTACTA AAGGCAGCAA ATCTCCAGCA 
AAGGTTGGTG AAGGAGGCAG TGGTATAGAC TTCAAAATGG TGTCCTCTGG CCTCACTGAA 
AACCAGCTAC AGCTCTCTGT AGAGGTGTTG ACATCCCACT CTTGTTCTGA AGAAGGCTTG 
GAAGATGCGG CCAATGTGTT ACTGCAGCTC TCTCGTGGGG ACTCTGGTAC CCGGGACACT 
GTTCTTAAGC TGCTACTAAA TGGAGCCCGC CATCTGGGTT ATACCCTCTG TAAACAGATA 
GGTACCCTAC TCGCTGAACT ACGGGAATAT AATCTGGAGC AGCAGCGGCG AGCCCAATGC 
GAGACCCTCT CTCCTGATGG CCTACCTGAG GAGCAACCAC AGACCACCAA ACTGAAAGGC 
AAAATGCAGA GCAGGTTTGA CATGGCTGAA AATGTGGTAA TTGTGGCATC TCAGAAGCGA 
CCCTTGGGTG GCCGGGAGCT CCAGCTGCCT TCTATGTCCA TGTTGACATC CAAGACATCT 
ACCCAGAAGT TCTTCTTGAG GGTACTACAG GTCATCATCC AGCTGCGGGA TGACACACGA 
CGAGCTAACA AGAAAGCCAA GCAGACAGGC AGGCTAGGTT CCTCCGGTTT AGGCTCAGCT 
AGCAGCATCC AGGCAGCTGT TCGGCAGCTG GAGGCTGAGG CTGATGCCAT TATACAAATG 
GTACGTGAGG GTCAAAGGGC GCGGAGACAG CAACAAGCAG CAACGTCGGA GTCTTCTAAC 
CAGTCAGAGA CATCTGTCCG GAGAGAGGAA TCGCCCATGG ATGTGGACCA GCCATCTCCT 
AGTGCTCAAG ATACTCAGTC CATTGTAATC TCAGATGGAA CACCACAGGG AGAGAAGGAG 
AAAGAGGAGA AACCACCTGA GTTACCCCTG CTCAGTGAGC AACTGAGCCT AGATGAACTG 
TGGGACATGC TAGGGGAATG TTTAAAAGAA CTGGAGGAAT CTCATGACCA GCATGCAGTA 
TTAGTGCTAC AGCCTGCTGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC 
AAGCCTCCTG TCCGAGATAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA TGAACCTCCT 
CCACTCTCCC CCGCCCCCTT AACCCCAGCC ACTCCATCCT CCCTTGACCC ATTCTTCTCC 
AGGGAGCCCT CATCTATGCA CATTTCCTCG AGCCTGCCCC CTGACACACA GAAGTTCCTT 
CGCTTTGCAG AGACTCACCG CACTGTGTTA AACCAGATAC TACGGCAGTC TACCACCCAT 
CTTGCTGATG GGCCTTTTGC TGTCTTGGTA GATTATATCC GTGTCCTCGA CTTTGATGTC 
AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG CGTTTGGACG AAGGGCTCCG GAAAGAAGAT 
ATGGCAGTGC ATGTCCGCCG TGACCATGTA TTTGAAGACT CCTATCGTGA GCTGCATCGC 
AAATCTCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG 
GATGCTGGGG GGCTCCTGCG AGAGTGGTAC ATGATCATCT CTCGAGAGAT GTTCAACCCT 
ATGTATGCCT TGTTCCGTAC CTCACCTGGT GACCGGGTCA CCTACACCAT CAATCCATCT 
TCCCACTGTA ACCCCAACCA CCTCAGCTAC TTCAAGTTTG TTGGACGAAT TGTAGCCAAA 
GCTGTATATG ACAACCGCCT CCTGGAGTGC TACTTTACTA GGTCTTTCTA CAAACACATC 
TTGGGCAAAT CTGTCAGGTA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC 
CTAGTTTATC TGCTTGAAAA TGATGTCTCT ACATTAGGCT ATGACCTCAC CTTCAGCACT 
GAGGTTCAAG AATTTGGAGT ATGTGAAGTT CGTGACCTCA AACCCAATGG AGCCAACATC 
TTGGTAACAG AGGAGAACAA GAAGGAATAT GTACACCTGG TTTGCCAGAT GAGAATGACA 
GGAGCCATCC GCAAACAGCT GGCAGCCTTC TTGGAGGGCT TCTATGAAAT CATTCCAAAG 
CGCCTCATAT CCATCTTTAC TGAGCAGGAA TTAGAGCTGC TCATATCAGG GCTGCCTACC 
ATCGACATTG ATGATCTAAA ATCTAACACT GAGTACCACA AGTACCAATC CAACTCTATT 
CAGATCCAGT GGTTTTGGAG AGCATTGCGT TCCTTTGACC AAGCAGACCG TGCCAAGTTC 
CTCCAGTTTG TCACAGGTAC TTCCAAGGTA CCCCTACAAG GATTTGCTGC CCTTGAAGGT 
ATGAATGGCA TTCAGAAGTT TCAGATTCAT CGAGATGACA GATCCACAGA TCGCCTGCCT 
TCAGCTCACA CATGTTTTAA TCAACTGGAC CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC 
CGCCACATGC TACTATTGGC CATCCAGGAA TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_011247858.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246160.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X18, mRNA.

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CloneID	OMu15194
Clone ID Related Accession (Same CDS sequence)	XM_011247858.1 , XM_006528949.4 , XM_030251455.1 , XM_030251456.1 , XM_006528949.3 , NM_021523.4
Accession Version	XM_011247858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGAGAAC AGGATGGAAC TGCTGGAAGC 
ATGGATGCAT CTGCCCAGGG TTTATTAGAA GGAATTGAAC TAGATGGTGA CACACTGGCA 
CCCATGGAGA CAGATGAACC TTCTTCTTCA GATTCTAAGG GCAAATCTAA AATCACACCT 
GCCATGGCTG CAAGAATTAA ACAAATCAAG CCGTTGCTGT CAGCTTCTTC CAGATTAGGC 
CGAGCACTTG CTGAGCTCTT TGGACTCCTT GTTAAGCTTT GTGTGGGTTC TCCTGTCCGC 
CAAAGGAGGA GCCATCATGC TGCCAGTACC ACCACTGCAC CAACACCTGC TGCTAGATCA 
ACAGCTTCAG CCCTCACTAA GCTACTCACA AAAGGCTTAT CATGGCAGCC CCCACCATAT 
ACACCCACTC CCCGCTTCAG GCTGACGTTC TTCATCTGTT CAGTTGGATT CACCTCTCCA 
ATGCTCTTTG ATGAGAGAAA GTATCCTTAC CACCTTATGC TGCAGAAATT TCTCTGCTCT 
GGTGGTCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TATCCATGGG AGGAAAAGTT 
CCTGTTTCTG AGGGATTGGA ACATTCAGAT CTACCTGATG GTACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTTGTAGA AAAGATGGTA AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGTTGCCTG CTAAATTGCC TGGAGGAGTC CAGAGCTTTC CACAATTTAG TGCCCTTCGC 
TTCCTTGTAG TAACTCAAAA AGCAGCCTTT ACTTGTATCA AGAACCTGTG GAACCGGAAA 
CCCCTTAAAG TATATGGGGG AAGAATGGCT GAGTCTATGC TGGCCATTTT GTGCCACATT 
CTCCGAGGGG AACCTGTGAT TCGAGAAAGA CTGAGCAAAG AGAAGGAAGG ATCTCGAGGA 
GAAGAAGAAG CAGGCCAGGA AGAAGGTGGC TCACGCAGAG AACCTCAAGT CAACCAGCAA 
CAGCTACAAC AGCTCATGGA CATGGGTTTC ACAAGAGAAC ATGCCATGGA GGCACTGTTG 
AATACCAGTA CGATGGAACA AGCAACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATA 
GGAGGAGTTG TTCGGGATCT TAGCATGTCA GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAAGA GCGTAAAGCT CGAGAAAAAC AAGAAGAGGA AGAAGCTAAA 
TGTCTAGAGA AGTTTCAAGA TGCTGACCCC TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACAATGT TACCAGGCTG CTTCCATCTT CTTGATGAGT TGCCTGATAC AGTGTACCGT 
GTTTGTGATC TGATCATGAC AGCTATCAAA CGTAATGGAG CTGACTATCG TGATATGATT 
CTAAAGCAAG TTGTCAACCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCTCTAACAA CAAGTGACAC AAAAACAGTT TCAGAGTGGA TCAGTCAGAT GGCTACCCTG 
CCACAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CACTACTTTT TGAGGAGTTG 
AAGTTACCTT GTGCCTGGGT GGTTGAATCA AGTGGCATTC TTAATGTCCT AATCAAACTC 
TTAGAAGTGG TTCAACCCTG CCTACAGGCT GCCAAAGAGC AAAAGGAAGT CCAGACCCCA 
AAGTGGATCA CCCCAGTGTT GCTCCTTATT GATTTTTATG AAAAGACAGC CATCTCATCA 
AAGAGAAGAG CCCAAATGAC TAAGTACCTA CAGTCCAACA GCAACAACTG GCGCTGGTTT 
GATGACCGCT CTGGGCGTTG GTGTAGTTAC AGTGCTAGCA ACAACAGCAC CATTGATTCT 
GCCTGGAAAT CTGGAGAAAC AAGTGTAAGA TTCACTGCTG GCCGACGAAG ATACACTGTC 
CAGTTCACTA CAATGGTGCA GGTTAATGAA GAAACAGGAA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTGCCACG ACTGAGTAAG AACTCAAAAA GCAGCAATGG ACAGGAACTA 
GAGAAGACAC TGGAAGAAAG CAAGGAGACC GATATCAAAC ATAAAGAAAA TAAAGGCAAT 
GATATCCCCT TAGCTCTAGA GAGCACAAAC ACTGAAAAAG AGGCAAGCCT GGATGAAACA 
AAAATTGGGG AGATCCTAAT CCAGGGCCTG ACAGAAGATA TGGTGACTGT TCTAATCCGT 
GCCTGTGTGA GCATGCTGGG AGTCCCTGTG GACCCAGACA CTTTGCATGC CACCCTTCGT 
CTCTGTTTAA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACACGAATGA TCTTGAATTT GACCCAGAGC TCAGGTTTCA ATGGGTTTAC CCCCTTAGTC 
ACCCTTCTCT TAAGGCACAT CATTGAGGAT CCTTGTACTC TTCGTCATAC TATGGAAAAG 
GTTGTTCGCT CGGCAGCCAC AAGTGGAGCT GGTAGTACTA CATCTGGTGT TGTATCTGGC 
AGCCTCGGTT CTCGGGAGAT CAACTATATC CTTAGAGTCC TAGGACCAGC TGCCTGCCGC 
AATCCAGACA TATTCACAGA AGTAGCCAAT TGCTGTATCC GCATTGCCCT TCCAGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGTCCTAAT 
GCTGTACAGC TGGTAAAGAC AACACCTTTG AAGCCCTCAT CTCTGCCTGT CATTCCTGAT 
ACTATTAAGG AAGTTATCTA TGATATGCTG AATGCTTTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGACA AATCTGATCC TAAACCTGGG GGAACGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGACGATGT ATACCAGCAG TACCGGTCAC TTACTCGTCA GAGCAGTGAC 
TTTGATACAC AGTCAGGTTT TTCCTTAAAT AGTCAGGTTT TTGCTGCAGA TGGTGCCCCT 
GCTGAAACTT CCACAACTGG AACCTCCCAA GGAGAGGGAG CTTCAACTCC AGAGGAGACT 
CGAGAAGGGA AGAAAGATAA AGAAGGGGAC CGTACATCTG AGGAAGGCAA GCAGAAAAGC 
AAGGGCAGCA AACCTTTAAT GCCTACCTCT ACTATCCTTC GTCTTCTGGC AGAGTTGGTG 
AGGTCCTATG TTGGTATTGC TACCCTGATT GCAAACTACA GCTATACCGT GGGCCAGTCT 
GAGCTGATCA AAGAGGACTG CAGTGTGCTA GCTTTTGTCC TGGACCACCT CCTCCCACAC 
ACCCAGAATG CAGAAGACAA GGATACACCT GCCCTGGCAC GTCTATTCCT TGCAAGTCTG 
GCTGCTGCGG GGAGTGGCAC AGATGCTCAA GTGGCCCTAG TGAATGAAGT GAAAGCAGCC 
CTGGGACGGG CGCTTGCTAT GGCTGAGAGT ACAGAGAAGC ACGCCAGACT TCAGGCAGTG 
ATGTGTATCA TCAGTACTAT CATGGAATCC TGCCCCTCCA CCTCCAGCTT CTACAGTAGT 
GCTACAGCCA AGACTCAGCA TAATGGCATG AACAACATCA TTCGACTGTT CCTAAAGAAG 
GGACTAGTCA ATGACCTGGC CAGAGTTCCT CACAGCCTAG ACCTGTCCAG TCCCAATATG 
GCCAACACAG TCAATGCTGC TCTAAAACCC TTGGAAACAC TTTCCCGGAT TGTAAATCAA 
CCCAGTAGCC TTTTTGGCAG CAAGAGTGCT TCTAGCAAGA ACAAGTCTGA GCAGGATGCT 
CAAGGAGCTT CTCAGGACTC CAGCAGCCAT CAGCAAGATC CAGGCGAGCC TGGGGAGGCA 
GAAGTGCAGG AGGAGGATCA TGATGTCACT CAGACAGAGG TGGCAGATGG GGATATCATG 
GATGGGGAGG CTGAAACCGA CTCAGTGGTG ATTGCCGGGC AGCCTGAGGT GCTCAGTTCA 
CAAGAGATGC AGGTTGAAAA TGAACTGGAG GACCTGATAG ATGAGTTGCT TGAGAGGGAT 
GGCGGATCTG GGAACAGTAC AATTATAGTG AGCAGAAGTG GAGAGGATGA ATCACAAGAG 
GACGTGCTGA TGGATGAAGC TCCTTCCAAC CTCAGCCAAG CTTCCACCTT GCAGGCCAAC 
CGAGAAGATT CCATGAATAT CCTGGACCCT GAGGATGAGG AGGAACATAC TCAGGAAGAG 
GACAGCAGTG GCAGTAACGA GGATGAGGAT GATAGTCAGG ATGAAGAGGA GGAGGAGGAG 
GAAGATGAGG AAGATGATCA GGAGGATGAT GAAGGTGAAG AAGGAGATGA AGATGATGAC 
GACGATGGTT CTGAGATGGA ATTGGATGAG GATTACCCTG ATATGAACGC TTCTCCCTTG 
GTCCGATTTG AGCGCTTTGA CCGGGAGGAT GATCTCATCA TTGAGTTTGA CAACATGTTC 
TCCAGTGCTA CAGACATTCC CCCATCCCCA GGAAATATCC CTACCACCCA CCCATTGATG 
GTACGTCATG CAGATCACAG TTCCCTGACA CTGGGCAGTG GTTCTTCCAC AACTCGTCTT 
ACCCAGGGCA TCGGTCGCAG TCAAAGGACC CTCAGACAGC TCACTGCCAA TACAGGCCAT 
ACCATTCATG TTCACTACCC TGGGAATCGC CAGCCTAACC CTCCTCTTAT ACTCCAGAGG 
TTGCTTGGTC CCTCTGCAGC TGCTGACATT CTTCAGCTGA GCAGCAGCCT TCCATTACAA 
AGCCGAGGCC GGGCCCGCCT CCTAGTGGGC AATGATGACG TCCATATCAT CGCCAGGTCT 
GATGACGAGC TGCTGGATGA CTTTTTTCAT GACCAGAGTA CAGCCACTAG CCAAGCAGGA 
ACCCTGTCCA GCATCCCAAC AGCTCTAACC CGATGGACAG AGGAATGCAA AGTTCTTGAT 
GCTGAGAGTA TGCATGACTG TGTTTCAGTG GTTAAAGTGC CCATTGTCAA TCACCTGGAA 
TTCTTGAGAG ATGAGGAGCT AGAAGAGAGA AGGGAGAAAC GCAGGAAACA ATTGGCTGAG 
GAAGAAACAA AAATAATAGA CAAAGGCAAA GAAGACAAGG AGAACAGAGA TCAGAGTGCA 
CAGTGTACTG TAACTAAGAC AAATGACTCC ACTGAACAGA ATGTCTCAGA TGGGACTCCT 
ATGCCTGACA GCTATCCAAC AACCCCCTCT TCAACGGATG CACCTACATC TGAGTCCAAG 
GAAACACTTG GTACCCTACA ACCCTCCCAG CAACAGCCAG CACTTCCACC CCCACCATCC 
TTAGGAGAGA TTCCTCAGGA ACTACAGTCC CCTGCTGAAG AAGTGGCAAA CTCTACACAG 
CTATTGATGC CTATAGAGTT GGAGGAATTG GGTCCCACAA GACCAAGTGG AGAAGCAGAA 
ACAACTCAAA TGGAATTGTC TCCAGCTCCC ACCATAACCT CTCTTTCCCC AGAGAGAGCT 
GAAGATTCAG ACGCGTTAAC AGCTGTCAGC AGTCAGCTGG AAGGTTCTCC CATGGACACC 
AGCAGCCTGG CTTCCTGTAC GTTAGAAGAG GCTGTGGGTG ACACTCCAGC AGCTGGCAGT 
TCTGAGCAGC CCACAGCAGG TAGTTCCACT CCTGGGGATG CGCCATCAGT TGTGGCAGAA 
GTGCAAGGCA GACCTGATGT ATCAAGAGAA TCCAACCAGC CACCTGAGGA CAGCTCCCCC 
CCTGCATCCT CGGAGAGTTC TTCCACTAGA GATTCTGCCG TGGCCATCTC TGGAGCAGAT 
TCTCGTGGAA TTCTAGAAGA GCCACTACCT TCAACAAGCA GTGAAGAAGA AGATCCCCTT 
GCTGGTATCA GTCTCCCTGA AGGTGTGGAT CCCTCCTTTC TGGCTGCTCT CCCTGATGAT 
ATCCGTCGGG AAGTCCTACA GAACCAGCTG GGCATACGTC CACCAACCCG AAGTGCTCCC 
TCCTCAAATA GCTCAGCTCC TGCAGTAGTG GGGAACCCTG GTGTAACTGA AGTGAGCCCT 
GAGTTCCTGG CTGCCCTACC TCCAGCTATT CAAGAGGAAG TGCTAGCACA GCAGAGAGCT 
GAACAGCAGC GACGGGAACT GGCTCAGAAT GCGAGCTCAG ACACCCCCAT GGACCCTGTG 
ACTTTCATTC AGACTTTGCC CTCAGATCTG CGCCGTAGTG TCCTAGAGGA TATGGAAGAC 
AGTGTGTTAG CTGTGATGCC ACCTGACATT GCAGCTGAGG CTCAAGCTCT AAGACGAGAA 
CAAGAAGCCC GACAGCGGCA GCTCATGCAT GAGCGTCTTT TTGGACACAG TAGTACTTCT 
GCACTCTCTG CCATTCTCCG AAGCCCGGCT TTTACCAGTC GCCTGAGTGG CAACCGTGGG 
GTCCAGTATA CTCGCCTTGC TGTACAGAGG GGTGGCACCT TCCAGATGGG GGGTAGCAGC 
AGCCATAATA GGCCTTCTGG CAGTAATGTG GACACACTAC TCCGCCTCCG AGGACGACTC 
CTTCTAGACC ATGAAGCCCT GTCTTGCCTC TTGGTCCTAC TTTTTGTGGA TGAGCCAAAG 
CTCAATACTA GCCGTCTACA CCGAGTACTG AGAAATCTCT GCTATCATGC CCAGACCCGC 
CACTGGGTCA TCCGCAGCCT CCTGTCCATT TTGCAGCGCA GCAGTGAAAG TGAGTTATGC 
ATTGAGACAC CTAAGCTCTC CACAAGTGAA GAAAGGGGCA AGAAATCAAG CAAGAGCTGT 
GCATCAAGCA GTCATGAGAA CCGTCCCCTG GATCTGCTCC ACAAGATGGA GTCCAAAAGT 
TCCAATCAGC TCTCCTGGCT CTCAGTATCA ATGGATGCTG CTTTAGGCTG TAGGACTAAC 
ATATTCCAGA TTCAACGTTC TGGAGGGCGT AAACATACAG AGAAGCATGC AAGCAGTGGT 
TCCACTGTCC ACATCCACCC CCAGGCTGCT CCTGTTGTTT GCAGACATGT TTTGGATACA 
CTCATTCAGT TGGCCAAGGT GTTTCCCAGC CACTTTACAC AGCAGCGGAC CAAAGAAACA 
AACTGTGAGA GTGATCGGGA ACGGGGCAGT AAGCAGGCCT GCAGCCCATG CTCCTCACAG 
TCCTCCAGCA GTGGCATCTG TACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG 
AATGTTAGCC GAAAAGGCAA GAACTCCGTG AAGTCAGTAC CAGTGAGCTC TGGTGGTGAA 
GGGGAAACTT CCCCACACAG CCTTGAAGCC TCTCCACTGG GGCAGCTCAT GAACATGTTG 
TCACATCCAG TCATCCGCCG GAGTTCTCTC TTAACTGAGA AACTCCTGAG ACTCCTCTCT 
CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTATCAGAAG TGCAAACTAA CTCCAGCAAC 
AGTGGTTCCT CCACTGCTGC CACCTCAAAC ACATCTACTA CTACCACCAC CACCACTACT 
GCCACTGCCC CCACGCCCAC ACCCCCAGCT GCAACTACCC CTGTCACTTC TGCTCCAGCT 
CTGGTTGCTG CCACAGCTAT TTCCACCATT ACTGTAGCTG CTTCTACCAC AGTGACTACC 
CCCACAACTG CTACCACTAC TGTTTCAACT TCTACTACTA AAGGCAGCAA ATCTCCAGCA 
AAGGTTGGTG AAGGAGGCAG TGGTATAGAC TTCAAAATGG TGTCCTCTGG CCTCACTGAA 
AACCAGCTAC AGCTCTCTGT AGAGGTGTTG ACATCCCACT CTTGTTCTGA AGAAGGCTTG 
GAAGATGCGG CCAATGTGTT ACTGCAGCTC TCTCGTGGGG ACTCTGGTAC CCGGGACACT 
GTTCTTAAGC TGCTACTAAA TGGAGCCCGC CATCTGGGTT ATACCCTCTG TAAACAGATA 
GGTACCCTAC TCGCTGAACT ACGGGAATAT AATCTGGAGC AGCAGCGGCG AGCCCAATGC 
GAGACCCTCT CTCCTGATGG CCTACCTGAG GAGCAACCAC AGACCACCAA ACTGAAAGGC 
AAAATGCAGA GCAGGTTTGA CATGGCTGAA AATGTGGTAA TTGTGGCATC TCAGAAGCGA 
CCCTTGGGTG GCCGGGAGCT CCAGCTGCCT TCTATGTCCA TGTTGACATC CAAGACATCT 
ACCCAGAAGT TCTTCTTGAG GGTACTACAG GTCATCATCC AGCTGCGGGA TGACACACGA 
CGAGCTAACA AGAAAGCCAA GCAGACAGGC AGGCTAGGTT CCTCCGGTTT AGGCTCAGCT 
AGCAGCATCC AGGCAGCTGT TCGGCAGCTG GAGGCTGAGG CTGATGCCAT TATACAAATG 
GTACGTGAGG GTCAAAGGGC GCGGAGACAG CAACAAGCAG CAACGTCGGA GTCTTCTAAC 
CAGTCAGAGA CATCTGTCCG GAGAGAGGAA TCGCCCATGG ATGTGGACCA GCCATCTCCT 
AGTGCTCAAG ATACTCAGTC CATTGTAATC TCAGATGGAA CACCACAGGG AGAGAAGGAG 
AAAGAGGAGA AACCACCTGA GTTACCCCTG CTCAGTGAGC AACTGAGCCT AGATGAACTG 
TGGGACATGC TAGGGGAATG TTTAAAAGAA CTGGAGGAAT CTCATGACCA GCATGCAGTA 
TTAGTGCTAC AGCCTGCTGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC 
AAGCCTCCTG TCCGAGATAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA TGAACCTCCT 
CCACTCTCCC CCGCCCCCTT AACCCCAGCC ACTCCATCCT CCCTTGACCC ATTCTTCTCC 
AGGGAGCCCT CATCTATGCA CATTTCCTCG AGCCTGCCCC CTGACACACA GAAGTTCCTT 
CGCTTTGCAG AGACTCACCG CACTGTGTTA AACCAGATAC TACGGCAGTC TACCACCCAT 
CTTGCTGATG GGCCTTTTGC TGTCTTGGTA GATTATATCC GTGTCCTCGA CTTTGATGTC 
AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG CGTTTGGACG AAGGGCTCCG GAAAGAAGAT 
ATGGCAGTGC ATGTCCGCCG TGACCATGTA TTTGAAGACT CCTATCGTGA GCTGCATCGC 
AAATCTCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG 
GATGCTGGGG GGCTCCTGCG AGAGTGGTAC ATGATCATCT CTCGAGAGAT GTTCAACCCT 
ATGTATGCCT TGTTCCGTAC CTCACCTGGT GACCGGGTCA CCTACACCAT CAATCCATCT 
TCCCACTGTA ACCCCAACCA CCTCAGCTAC TTCAAGTTTG TTGGACGAAT TGTAGCCAAA 
GCTGTATATG ACAACCGCCT CCTGGAGTGC TACTTTACTA GGTCTTTCTA CAAACACATC 
TTGGGCAAAT CTGTCAGGTA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC 
CTAGTTTATC TGCTTGAAAA TGATGTCTCT ACATTAGGCT ATGACCTCAC CTTCAGCACT 
GAGGTTCAAG AATTTGGAGT ATGTGAAGTT CGTGACCTCA AACCCAATGG AGCCAACATC 
TTGGTAACAG AGGAGAACAA GAAGGAATAT GTACACCTGG TTTGCCAGAT GAGAATGACA 
GGAGCCATCC GCAAACAGCT GGCAGCCTTC TTGGAGGGCT TCTATGAAAT CATTCCAAAG 
CGCCTCATAT CCATCTTTAC TGAGCAGGAA TTAGAGCTGC TCATATCAGG GCTGCCTACC 
ATCGACATTG ATGATCTAAA ATCTAACACT GAGTACCACA AGTACCAATC CAACTCTATT 
CAGATCCAGT GGTTTTGGAG AGCATTGCGT TCCTTTGACC AAGCAGACCG TGCCAAGTTC 
CTCCAGTTTG TCACAGGTAC TTCCAAGGTA CCCCTACAAG GATTTGCTGC CCTTGAAGGT 
ATGAATGGCA TTCAGAAGTT TCAGATTCAT CGAGATGACA GATCCACAGA TCGCCTGCCT 
TCAGCTCACA CATGTTTTAA TCAACTGGAC CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC 
CGCCACATGC TACTATTGGC CATCCAGGAA TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246160.1
CDS	1191..14327
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_011247858.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247858.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGAGAAC AGGATGGAAC TGCTGGAAGC 
ATGGATGCAT CTGCCCAGGG TTTATTAGAA GGAATTGAAC TAGATGGTGA CACACTGGCA 
CCCATGGAGA CAGATGAACC TTCTTCTTCA GATTCTAAGG GCAAATCTAA AATCACACCT 
GCCATGGCTG CAAGAATTAA ACAAATCAAG CCGTTGCTGT CAGCTTCTTC CAGATTAGGC 
CGAGCACTTG CTGAGCTCTT TGGACTCCTT GTTAAGCTTT GTGTGGGTTC TCCTGTCCGC 
CAAAGGAGGA GCCATCATGC TGCCAGTACC ACCACTGCAC CAACACCTGC TGCTAGATCA 
ACAGCTTCAG CCCTCACTAA GCTACTCACA AAAGGCTTAT CATGGCAGCC CCCACCATAT 
ACACCCACTC CCCGCTTCAG GCTGACGTTC TTCATCTGTT CAGTTGGATT CACCTCTCCA 
ATGCTCTTTG ATGAGAGAAA GTATCCTTAC CACCTTATGC TGCAGAAATT TCTCTGCTCT 
GGTGGTCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TATCCATGGG AGGAAAAGTT 
CCTGTTTCTG AGGGATTGGA ACATTCAGAT CTACCTGATG GTACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTTGTAGA AAAGATGGTA AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGTTGCCTG CTAAATTGCC TGGAGGAGTC CAGAGCTTTC CACAATTTAG TGCCCTTCGC 
TTCCTTGTAG TAACTCAAAA AGCAGCCTTT ACTTGTATCA AGAACCTGTG GAACCGGAAA 
CCCCTTAAAG TATATGGGGG AAGAATGGCT GAGTCTATGC TGGCCATTTT GTGCCACATT 
CTCCGAGGGG AACCTGTGAT TCGAGAAAGA CTGAGCAAAG AGAAGGAAGG ATCTCGAGGA 
GAAGAAGAAG CAGGCCAGGA AGAAGGTGGC TCACGCAGAG AACCTCAAGT CAACCAGCAA 
CAGCTACAAC AGCTCATGGA CATGGGTTTC ACAAGAGAAC ATGCCATGGA GGCACTGTTG 
AATACCAGTA CGATGGAACA AGCAACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATA 
GGAGGAGTTG TTCGGGATCT TAGCATGTCA GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAAGA GCGTAAAGCT CGAGAAAAAC AAGAAGAGGA AGAAGCTAAA 
TGTCTAGAGA AGTTTCAAGA TGCTGACCCC TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACAATGT TACCAGGCTG CTTCCATCTT CTTGATGAGT TGCCTGATAC AGTGTACCGT 
GTTTGTGATC TGATCATGAC AGCTATCAAA CGTAATGGAG CTGACTATCG TGATATGATT 
CTAAAGCAAG TTGTCAACCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCTCTAACAA CAAGTGACAC AAAAACAGTT TCAGAGTGGA TCAGTCAGAT GGCTACCCTG 
CCACAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CACTACTTTT TGAGGAGTTG 
AAGTTACCTT GTGCCTGGGT GGTTGAATCA AGTGGCATTC TTAATGTCCT AATCAAACTC 
TTAGAAGTGG TTCAACCCTG CCTACAGGCT GCCAAAGAGC AAAAGGAAGT CCAGACCCCA 
AAGTGGATCA CCCCAGTGTT GCTCCTTATT GATTTTTATG AAAAGACAGC CATCTCATCA 
AAGAGAAGAG CCCAAATGAC TAAGTACCTA CAGTCCAACA GCAACAACTG GCGCTGGTTT 
GATGACCGCT CTGGGCGTTG GTGTAGTTAC AGTGCTAGCA ACAACAGCAC CATTGATTCT 
GCCTGGAAAT CTGGAGAAAC AAGTGTAAGA TTCACTGCTG GCCGACGAAG ATACACTGTC 
CAGTTCACTA CAATGGTGCA GGTTAATGAA GAAACAGGAA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTGCCACG ACTGAGTAAG AACTCAAAAA GCAGCAATGG ACAGGAACTA 
GAGAAGACAC TGGAAGAAAG CAAGGAGACC GATATCAAAC ATAAAGAAAA TAAAGGCAAT 
GATATCCCCT TAGCTCTAGA GAGCACAAAC ACTGAAAAAG AGGCAAGCCT GGATGAAACA 
AAAATTGGGG AGATCCTAAT CCAGGGCCTG ACAGAAGATA TGGTGACTGT TCTAATCCGT 
GCCTGTGTGA GCATGCTGGG AGTCCCTGTG GACCCAGACA CTTTGCATGC CACCCTTCGT 
CTCTGTTTAA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACACGAATGA TCTTGAATTT GACCCAGAGC TCAGGTTTCA ATGGGTTTAC CCCCTTAGTC 
ACCCTTCTCT TAAGGCACAT CATTGAGGAT CCTTGTACTC TTCGTCATAC TATGGAAAAG 
GTTGTTCGCT CGGCAGCCAC AAGTGGAGCT GGTAGTACTA CATCTGGTGT TGTATCTGGC 
AGCCTCGGTT CTCGGGAGAT CAACTATATC CTTAGAGTCC TAGGACCAGC TGCCTGCCGC 
AATCCAGACA TATTCACAGA AGTAGCCAAT TGCTGTATCC GCATTGCCCT TCCAGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGTCCTAAT 
GCTGTACAGC TGGTAAAGAC AACACCTTTG AAGCCCTCAT CTCTGCCTGT CATTCCTGAT 
ACTATTAAGG AAGTTATCTA TGATATGCTG AATGCTTTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGACA AATCTGATCC TAAACCTGGG GGAACGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGACGATGT ATACCAGCAG TACCGGTCAC TTACTCGTCA GAGCAGTGAC 
TTTGATACAC AGTCAGGTTT TTCCTTAAAT AGTCAGGTTT TTGCTGCAGA TGGTGCCCCT 
GCTGAAACTT CCACAACTGG AACCTCCCAA GGAGAGGGAG CTTCAACTCC AGAGGAGACT 
CGAGAAGGGA AGAAAGATAA AGAAGGGGAC CGTACATCTG AGGAAGGCAA GCAGAAAAGC 
AAGGGCAGCA AACCTTTAAT GCCTACCTCT ACTATCCTTC GTCTTCTGGC AGAGTTGGTG 
AGGTCCTATG TTGGTATTGC TACCCTGATT GCAAACTACA GCTATACCGT GGGCCAGTCT 
GAGCTGATCA AAGAGGACTG CAGTGTGCTA GCTTTTGTCC TGGACCACCT CCTCCCACAC 
ACCCAGAATG CAGAAGACAA GGATACACCT GCCCTGGCAC GTCTATTCCT TGCAAGTCTG 
GCTGCTGCGG GGAGTGGCAC AGATGCTCAA GTGGCCCTAG TGAATGAAGT GAAAGCAGCC 
CTGGGACGGG CGCTTGCTAT GGCTGAGAGT ACAGAGAAGC ACGCCAGACT TCAGGCAGTG 
ATGTGTATCA TCAGTACTAT CATGGAATCC TGCCCCTCCA CCTCCAGCTT CTACAGTAGT 
GCTACAGCCA AGACTCAGCA TAATGGCATG AACAACATCA TTCGACTGTT CCTAAAGAAG 
GGACTAGTCA ATGACCTGGC CAGAGTTCCT CACAGCCTAG ACCTGTCCAG TCCCAATATG 
GCCAACACAG TCAATGCTGC TCTAAAACCC TTGGAAACAC TTTCCCGGAT TGTAAATCAA 
CCCAGTAGCC TTTTTGGCAG CAAGAGTGCT TCTAGCAAGA ACAAGTCTGA GCAGGATGCT 
CAAGGAGCTT CTCAGGACTC CAGCAGCCAT CAGCAAGATC CAGGCGAGCC TGGGGAGGCA 
GAAGTGCAGG AGGAGGATCA TGATGTCACT CAGACAGAGG TGGCAGATGG GGATATCATG 
GATGGGGAGG CTGAAACCGA CTCAGTGGTG ATTGCCGGGC AGCCTGAGGT GCTCAGTTCA 
CAAGAGATGC AGGTTGAAAA TGAACTGGAG GACCTGATAG ATGAGTTGCT TGAGAGGGAT 
GGCGGATCTG GGAACAGTAC AATTATAGTG AGCAGAAGTG GAGAGGATGA ATCACAAGAG 
GACGTGCTGA TGGATGAAGC TCCTTCCAAC CTCAGCCAAG CTTCCACCTT GCAGGCCAAC 
CGAGAAGATT CCATGAATAT CCTGGACCCT GAGGATGAGG AGGAACATAC TCAGGAAGAG 
GACAGCAGTG GCAGTAACGA GGATGAGGAT GATAGTCAGG ATGAAGAGGA GGAGGAGGAG 
GAAGATGAGG AAGATGATCA GGAGGATGAT GAAGGTGAAG AAGGAGATGA AGATGATGAC 
GACGATGGTT CTGAGATGGA ATTGGATGAG GATTACCCTG ATATGAACGC TTCTCCCTTG 
GTCCGATTTG AGCGCTTTGA CCGGGAGGAT GATCTCATCA TTGAGTTTGA CAACATGTTC 
TCCAGTGCTA CAGACATTCC CCCATCCCCA GGAAATATCC CTACCACCCA CCCATTGATG 
GTACGTCATG CAGATCACAG TTCCCTGACA CTGGGCAGTG GTTCTTCCAC AACTCGTCTT 
ACCCAGGGCA TCGGTCGCAG TCAAAGGACC CTCAGACAGC TCACTGCCAA TACAGGCCAT 
ACCATTCATG TTCACTACCC TGGGAATCGC CAGCCTAACC CTCCTCTTAT ACTCCAGAGG 
TTGCTTGGTC CCTCTGCAGC TGCTGACATT CTTCAGCTGA GCAGCAGCCT TCCATTACAA 
AGCCGAGGCC GGGCCCGCCT CCTAGTGGGC AATGATGACG TCCATATCAT CGCCAGGTCT 
GATGACGAGC TGCTGGATGA CTTTTTTCAT GACCAGAGTA CAGCCACTAG CCAAGCAGGA 
ACCCTGTCCA GCATCCCAAC AGCTCTAACC CGATGGACAG AGGAATGCAA AGTTCTTGAT 
GCTGAGAGTA TGCATGACTG TGTTTCAGTG GTTAAAGTGC CCATTGTCAA TCACCTGGAA 
TTCTTGAGAG ATGAGGAGCT AGAAGAGAGA AGGGAGAAAC GCAGGAAACA ATTGGCTGAG 
GAAGAAACAA AAATAATAGA CAAAGGCAAA GAAGACAAGG AGAACAGAGA TCAGAGTGCA 
CAGTGTACTG TAACTAAGAC AAATGACTCC ACTGAACAGA ATGTCTCAGA TGGGACTCCT 
ATGCCTGACA GCTATCCAAC AACCCCCTCT TCAACGGATG CACCTACATC TGAGTCCAAG 
GAAACACTTG GTACCCTACA ACCCTCCCAG CAACAGCCAG CACTTCCACC CCCACCATCC 
TTAGGAGAGA TTCCTCAGGA ACTACAGTCC CCTGCTGAAG AAGTGGCAAA CTCTACACAG 
CTATTGATGC CTATAGAGTT GGAGGAATTG GGTCCCACAA GACCAAGTGG AGAAGCAGAA 
ACAACTCAAA TGGAATTGTC TCCAGCTCCC ACCATAACCT CTCTTTCCCC AGAGAGAGCT 
GAAGATTCAG ACGCGTTAAC AGCTGTCAGC AGTCAGCTGG AAGGTTCTCC CATGGACACC 
AGCAGCCTGG CTTCCTGTAC GTTAGAAGAG GCTGTGGGTG ACACTCCAGC AGCTGGCAGT 
TCTGAGCAGC CCACAGCAGG TAGTTCCACT CCTGGGGATG CGCCATCAGT TGTGGCAGAA 
GTGCAAGGCA GACCTGATGT ATCAAGAGAA TCCAACCAGC CACCTGAGGA CAGCTCCCCC 
CCTGCATCCT CGGAGAGTTC TTCCACTAGA GATTCTGCCG TGGCCATCTC TGGAGCAGAT 
TCTCGTGGAA TTCTAGAAGA GCCACTACCT TCAACAAGCA GTGAAGAAGA AGATCCCCTT 
GCTGGTATCA GTCTCCCTGA AGGTGTGGAT CCCTCCTTTC TGGCTGCTCT CCCTGATGAT 
ATCCGTCGGG AAGTCCTACA GAACCAGCTG GGCATACGTC CACCAACCCG AAGTGCTCCC 
TCCTCAAATA GCTCAGCTCC TGCAGTAGTG GGGAACCCTG GTGTAACTGA AGTGAGCCCT 
GAGTTCCTGG CTGCCCTACC TCCAGCTATT CAAGAGGAAG TGCTAGCACA GCAGAGAGCT 
GAACAGCAGC GACGGGAACT GGCTCAGAAT GCGAGCTCAG ACACCCCCAT GGACCCTGTG 
ACTTTCATTC AGACTTTGCC CTCAGATCTG CGCCGTAGTG TCCTAGAGGA TATGGAAGAC 
AGTGTGTTAG CTGTGATGCC ACCTGACATT GCAGCTGAGG CTCAAGCTCT AAGACGAGAA 
CAAGAAGCCC GACAGCGGCA GCTCATGCAT GAGCGTCTTT TTGGACACAG TAGTACTTCT 
GCACTCTCTG CCATTCTCCG AAGCCCGGCT TTTACCAGTC GCCTGAGTGG CAACCGTGGG 
GTCCAGTATA CTCGCCTTGC TGTACAGAGG GGTGGCACCT TCCAGATGGG GGGTAGCAGC 
AGCCATAATA GGCCTTCTGG CAGTAATGTG GACACACTAC TCCGCCTCCG AGGACGACTC 
CTTCTAGACC ATGAAGCCCT GTCTTGCCTC TTGGTCCTAC TTTTTGTGGA TGAGCCAAAG 
CTCAATACTA GCCGTCTACA CCGAGTACTG AGAAATCTCT GCTATCATGC CCAGACCCGC 
CACTGGGTCA TCCGCAGCCT CCTGTCCATT TTGCAGCGCA GCAGTGAAAG TGAGTTATGC 
ATTGAGACAC CTAAGCTCTC CACAAGTGAA GAAAGGGGCA AGAAATCAAG CAAGAGCTGT 
GCATCAAGCA GTCATGAGAA CCGTCCCCTG GATCTGCTCC ACAAGATGGA GTCCAAAAGT 
TCCAATCAGC TCTCCTGGCT CTCAGTATCA ATGGATGCTG CTTTAGGCTG TAGGACTAAC 
ATATTCCAGA TTCAACGTTC TGGAGGGCGT AAACATACAG AGAAGCATGC AAGCAGTGGT 
TCCACTGTCC ACATCCACCC CCAGGCTGCT CCTGTTGTTT GCAGACATGT TTTGGATACA 
CTCATTCAGT TGGCCAAGGT GTTTCCCAGC CACTTTACAC AGCAGCGGAC CAAAGAAACA 
AACTGTGAGA GTGATCGGGA ACGGGGCAGT AAGCAGGCCT GCAGCCCATG CTCCTCACAG 
TCCTCCAGCA GTGGCATCTG TACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG 
AATGTTAGCC GAAAAGGCAA GAACTCCGTG AAGTCAGTAC CAGTGAGCTC TGGTGGTGAA 
GGGGAAACTT CCCCACACAG CCTTGAAGCC TCTCCACTGG GGCAGCTCAT GAACATGTTG 
TCACATCCAG TCATCCGCCG GAGTTCTCTC TTAACTGAGA AACTCCTGAG ACTCCTCTCT 
CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTATCAGAAG TGCAAACTAA CTCCAGCAAC 
AGTGGTTCCT CCACTGCTGC CACCTCAAAC ACATCTACTA CTACCACCAC CACCACTACT 
GCCACTGCCC CCACGCCCAC ACCCCCAGCT GCAACTACCC CTGTCACTTC TGCTCCAGCT 
CTGGTTGCTG CCACAGCTAT TTCCACCATT ACTGTAGCTG CTTCTACCAC AGTGACTACC 
CCCACAACTG CTACCACTAC TGTTTCAACT TCTACTACTA AAGGCAGCAA ATCTCCAGCA 
AAGGTTGGTG AAGGAGGCAG TGGTATAGAC TTCAAAATGG TGTCCTCTGG CCTCACTGAA 
AACCAGCTAC AGCTCTCTGT AGAGGTGTTG ACATCCCACT CTTGTTCTGA AGAAGGCTTG 
GAAGATGCGG CCAATGTGTT ACTGCAGCTC TCTCGTGGGG ACTCTGGTAC CCGGGACACT 
GTTCTTAAGC TGCTACTAAA TGGAGCCCGC CATCTGGGTT ATACCCTCTG TAAACAGATA 
GGTACCCTAC TCGCTGAACT ACGGGAATAT AATCTGGAGC AGCAGCGGCG AGCCCAATGC 
GAGACCCTCT CTCCTGATGG CCTACCTGAG GAGCAACCAC AGACCACCAA ACTGAAAGGC 
AAAATGCAGA GCAGGTTTGA CATGGCTGAA AATGTGGTAA TTGTGGCATC TCAGAAGCGA 
CCCTTGGGTG GCCGGGAGCT CCAGCTGCCT TCTATGTCCA TGTTGACATC CAAGACATCT 
ACCCAGAAGT TCTTCTTGAG GGTACTACAG GTCATCATCC AGCTGCGGGA TGACACACGA 
CGAGCTAACA AGAAAGCCAA GCAGACAGGC AGGCTAGGTT CCTCCGGTTT AGGCTCAGCT 
AGCAGCATCC AGGCAGCTGT TCGGCAGCTG GAGGCTGAGG CTGATGCCAT TATACAAATG 
GTACGTGAGG GTCAAAGGGC GCGGAGACAG CAACAAGCAG CAACGTCGGA GTCTTCTAAC 
CAGTCAGAGA CATCTGTCCG GAGAGAGGAA TCGCCCATGG ATGTGGACCA GCCATCTCCT 
AGTGCTCAAG ATACTCAGTC CATTGTAATC TCAGATGGAA CACCACAGGG AGAGAAGGAG 
AAAGAGGAGA AACCACCTGA GTTACCCCTG CTCAGTGAGC AACTGAGCCT AGATGAACTG 
TGGGACATGC TAGGGGAATG TTTAAAAGAA CTGGAGGAAT CTCATGACCA GCATGCAGTA 
TTAGTGCTAC AGCCTGCTGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC 
AAGCCTCCTG TCCGAGATAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA TGAACCTCCT 
CCACTCTCCC CCGCCCCCTT AACCCCAGCC ACTCCATCCT CCCTTGACCC ATTCTTCTCC 
AGGGAGCCCT CATCTATGCA CATTTCCTCG AGCCTGCCCC CTGACACACA GAAGTTCCTT 
CGCTTTGCAG AGACTCACCG CACTGTGTTA AACCAGATAC TACGGCAGTC TACCACCCAT 
CTTGCTGATG GGCCTTTTGC TGTCTTGGTA GATTATATCC GTGTCCTCGA CTTTGATGTC 
AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG CGTTTGGACG AAGGGCTCCG GAAAGAAGAT 
ATGGCAGTGC ATGTCCGCCG TGACCATGTA TTTGAAGACT CCTATCGTGA GCTGCATCGC 
AAATCTCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG 
GATGCTGGGG GGCTCCTGCG AGAGTGGTAC ATGATCATCT CTCGAGAGAT GTTCAACCCT 
ATGTATGCCT TGTTCCGTAC CTCACCTGGT GACCGGGTCA CCTACACCAT CAATCCATCT 
TCCCACTGTA ACCCCAACCA CCTCAGCTAC TTCAAGTTTG TTGGACGAAT TGTAGCCAAA 
GCTGTATATG ACAACCGCCT CCTGGAGTGC TACTTTACTA GGTCTTTCTA CAAACACATC 
TTGGGCAAAT CTGTCAGGTA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC 
CTAGTTTATC TGCTTGAAAA TGATGTCTCT ACATTAGGCT ATGACCTCAC CTTCAGCACT 
GAGGTTCAAG AATTTGGAGT ATGTGAAGTT CGTGACCTCA AACCCAATGG AGCCAACATC 
TTGGTAACAG AGGAGAACAA GAAGGAATAT GTACACCTGG TTTGCCAGAT GAGAATGACA 
GGAGCCATCC GCAAACAGCT GGCAGCCTTC TTGGAGGGCT TCTATGAAAT CATTCCAAAG 
CGCCTCATAT CCATCTTTAC TGAGCAGGAA TTAGAGCTGC TCATATCAGG GCTGCCTACC 
ATCGACATTG ATGATCTAAA ATCTAACACT GAGTACCACA AGTACCAATC CAACTCTATT 
CAGATCCAGT GGTTTTGGAG AGCATTGCGT TCCTTTGACC AAGCAGACCG TGCCAAGTTC 
CTCCAGTTTG TCACAGGTAC TTCCAAGGTA CCCCTACAAG GATTTGCTGC CCTTGAAGGT 
ATGAATGGCA TTCAGAAGTT TCAGATTCAT CGAGATGACA GATCCACAGA TCGCCTGCCT 
TCAGCTCACA CATGTTTTAA TCAACTGGAC CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC 
CGCCACATGC TACTATTGGC CATCCAGGAA TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528949.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529012.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X32, mRNA.

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CloneID	OMu15194
Clone ID Related Accession (Same CDS sequence)	XM_011247858.1 , XM_006528949.4 , XM_030251455.1 , XM_030251456.1 , XM_006528949.3 , NM_021523.4
Accession Version	XM_006528949.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528949.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGAGAAC AGGATGGAAC TGCTGGAAGC 
ATGGATGCAT CTGCCCAGGG TTTATTAGAA GGAATTGAAC TAGATGGTGA CACACTGGCA 
CCCATGGAGA CAGATGAACC TTCTTCTTCA GATTCTAAGG GCAAATCTAA AATCACACCT 
GCCATGGCTG CAAGAATTAA ACAAATCAAG CCGTTGCTGT CAGCTTCTTC CAGATTAGGC 
CGAGCACTTG CTGAGCTCTT TGGACTCCTT GTTAAGCTTT GTGTGGGTTC TCCTGTCCGC 
CAAAGGAGGA GCCATCATGC TGCCAGTACC ACCACTGCAC CAACACCTGC TGCTAGATCA 
ACAGCTTCAG CCCTCACTAA GCTACTCACA AAAGGCTTAT CATGGCAGCC CCCACCATAT 
ACACCCACTC CCCGCTTCAG GCTGACGTTC TTCATCTGTT CAGTTGGATT CACCTCTCCA 
ATGCTCTTTG ATGAGAGAAA GTATCCTTAC CACCTTATGC TGCAGAAATT TCTCTGCTCT 
GGTGGTCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TATCCATGGG AGGAAAAGTT 
CCTGTTTCTG AGGGATTGGA ACATTCAGAT CTACCTGATG GTACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTTGTAGA AAAGATGGTA AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGTTGCCTG CTAAATTGCC TGGAGGAGTC CAGAGCTTTC CACAATTTAG TGCCCTTCGC 
TTCCTTGTAG TAACTCAAAA AGCAGCCTTT ACTTGTATCA AGAACCTGTG GAACCGGAAA 
CCCCTTAAAG TATATGGGGG AAGAATGGCT GAGTCTATGC TGGCCATTTT GTGCCACATT 
CTCCGAGGGG AACCTGTGAT TCGAGAAAGA CTGAGCAAAG AGAAGGAAGG ATCTCGAGGA 
GAAGAAGAAG CAGGCCAGGA AGAAGGTGGC TCACGCAGAG AACCTCAAGT CAACCAGCAA 
CAGCTACAAC AGCTCATGGA CATGGGTTTC ACAAGAGAAC ATGCCATGGA GGCACTGTTG 
AATACCAGTA CGATGGAACA AGCAACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATA 
GGAGGAGTTG TTCGGGATCT TAGCATGTCA GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAAGA GCGTAAAGCT CGAGAAAAAC AAGAAGAGGA AGAAGCTAAA 
TGTCTAGAGA AGTTTCAAGA TGCTGACCCC TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACAATGT TACCAGGCTG CTTCCATCTT CTTGATGAGT TGCCTGATAC AGTGTACCGT 
GTTTGTGATC TGATCATGAC AGCTATCAAA CGTAATGGAG CTGACTATCG TGATATGATT 
CTAAAGCAAG TTGTCAACCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCTCTAACAA CAAGTGACAC AAAAACAGTT TCAGAGTGGA TCAGTCAGAT GGCTACCCTG 
CCACAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CACTACTTTT TGAGGAGTTG 
AAGTTACCTT GTGCCTGGGT GGTTGAATCA AGTGGCATTC TTAATGTCCT AATCAAACTC 
TTAGAAGTGG TTCAACCCTG CCTACAGGCT GCCAAAGAGC AAAAGGAAGT CCAGACCCCA 
AAGTGGATCA CCCCAGTGTT GCTCCTTATT GATTTTTATG AAAAGACAGC CATCTCATCA 
AAGAGAAGAG CCCAAATGAC TAAGTACCTA CAGTCCAACA GCAACAACTG GCGCTGGTTT 
GATGACCGCT CTGGGCGTTG GTGTAGTTAC AGTGCTAGCA ACAACAGCAC CATTGATTCT 
GCCTGGAAAT CTGGAGAAAC AAGTGTAAGA TTCACTGCTG GCCGACGAAG ATACACTGTC 
CAGTTCACTA CAATGGTGCA GGTTAATGAA GAAACAGGAA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTGCCACG ACTGAGTAAG AACTCAAAAA GCAGCAATGG ACAGGAACTA 
GAGAAGACAC TGGAAGAAAG CAAGGAGACC GATATCAAAC ATAAAGAAAA TAAAGGCAAT 
GATATCCCCT TAGCTCTAGA GAGCACAAAC ACTGAAAAAG AGGCAAGCCT GGATGAAACA 
AAAATTGGGG AGATCCTAAT CCAGGGCCTG ACAGAAGATA TGGTGACTGT TCTAATCCGT 
GCCTGTGTGA GCATGCTGGG AGTCCCTGTG GACCCAGACA CTTTGCATGC CACCCTTCGT 
CTCTGTTTAA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACACGAATGA TCTTGAATTT GACCCAGAGC TCAGGTTTCA ATGGGTTTAC CCCCTTAGTC 
ACCCTTCTCT TAAGGCACAT CATTGAGGAT CCTTGTACTC TTCGTCATAC TATGGAAAAG 
GTTGTTCGCT CGGCAGCCAC AAGTGGAGCT GGTAGTACTA CATCTGGTGT TGTATCTGGC 
AGCCTCGGTT CTCGGGAGAT CAACTATATC CTTAGAGTCC TAGGACCAGC TGCCTGCCGC 
AATCCAGACA TATTCACAGA AGTAGCCAAT TGCTGTATCC GCATTGCCCT TCCAGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGTCCTAAT 
GCTGTACAGC TGGTAAAGAC AACACCTTTG AAGCCCTCAT CTCTGCCTGT CATTCCTGAT 
ACTATTAAGG AAGTTATCTA TGATATGCTG AATGCTTTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGACA AATCTGATCC TAAACCTGGG GGAACGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGACGATGT ATACCAGCAG TACCGGTCAC TTACTCGTCA GAGCAGTGAC 
TTTGATACAC AGTCAGGTTT TTCCTTAAAT AGTCAGGTTT TTGCTGCAGA TGGTGCCCCT 
GCTGAAACTT CCACAACTGG AACCTCCCAA GGAGAGGGAG CTTCAACTCC AGAGGAGACT 
CGAGAAGGGA AGAAAGATAA AGAAGGGGAC CGTACATCTG AGGAAGGCAA GCAGAAAAGC 
AAGGGCAGCA AACCTTTAAT GCCTACCTCT ACTATCCTTC GTCTTCTGGC AGAGTTGGTG 
AGGTCCTATG TTGGTATTGC TACCCTGATT GCAAACTACA GCTATACCGT GGGCCAGTCT 
GAGCTGATCA AAGAGGACTG CAGTGTGCTA GCTTTTGTCC TGGACCACCT CCTCCCACAC 
ACCCAGAATG CAGAAGACAA GGATACACCT GCCCTGGCAC GTCTATTCCT TGCAAGTCTG 
GCTGCTGCGG GGAGTGGCAC AGATGCTCAA GTGGCCCTAG TGAATGAAGT GAAAGCAGCC 
CTGGGACGGG CGCTTGCTAT GGCTGAGAGT ACAGAGAAGC ACGCCAGACT TCAGGCAGTG 
ATGTGTATCA TCAGTACTAT CATGGAATCC TGCCCCTCCA CCTCCAGCTT CTACAGTAGT 
GCTACAGCCA AGACTCAGCA TAATGGCATG AACAACATCA TTCGACTGTT CCTAAAGAAG 
GGACTAGTCA ATGACCTGGC CAGAGTTCCT CACAGCCTAG ACCTGTCCAG TCCCAATATG 
GCCAACACAG TCAATGCTGC TCTAAAACCC TTGGAAACAC TTTCCCGGAT TGTAAATCAA 
CCCAGTAGCC TTTTTGGCAG CAAGAGTGCT TCTAGCAAGA ACAAGTCTGA GCAGGATGCT 
CAAGGAGCTT CTCAGGACTC CAGCAGCCAT CAGCAAGATC CAGGCGAGCC TGGGGAGGCA 
GAAGTGCAGG AGGAGGATCA TGATGTCACT CAGACAGAGG TGGCAGATGG GGATATCATG 
GATGGGGAGG CTGAAACCGA CTCAGTGGTG ATTGCCGGGC AGCCTGAGGT GCTCAGTTCA 
CAAGAGATGC AGGTTGAAAA TGAACTGGAG GACCTGATAG ATGAGTTGCT TGAGAGGGAT 
GGCGGATCTG GGAACAGTAC AATTATAGTG AGCAGAAGTG GAGAGGATGA ATCACAAGAG 
GACGTGCTGA TGGATGAAGC TCCTTCCAAC CTCAGCCAAG CTTCCACCTT GCAGGCCAAC 
CGAGAAGATT CCATGAATAT CCTGGACCCT GAGGATGAGG AGGAACATAC TCAGGAAGAG 
GACAGCAGTG GCAGTAACGA GGATGAGGAT GATAGTCAGG ATGAAGAGGA GGAGGAGGAG 
GAAGATGAGG AAGATGATCA GGAGGATGAT GAAGGTGAAG AAGGAGATGA AGATGATGAC 
GACGATGGTT CTGAGATGGA ATTGGATGAG GATTACCCTG ATATGAACGC TTCTCCCTTG 
GTCCGATTTG AGCGCTTTGA CCGGGAGGAT GATCTCATCA TTGAGTTTGA CAACATGTTC 
TCCAGTGCTA CAGACATTCC CCCATCCCCA GGAAATATCC CTACCACCCA CCCATTGATG 
GTACGTCATG CAGATCACAG TTCCCTGACA CTGGGCAGTG GTTCTTCCAC AACTCGTCTT 
ACCCAGGGCA TCGGTCGCAG TCAAAGGACC CTCAGACAGC TCACTGCCAA TACAGGCCAT 
ACCATTCATG TTCACTACCC TGGGAATCGC CAGCCTAACC CTCCTCTTAT ACTCCAGAGG 
TTGCTTGGTC CCTCTGCAGC TGCTGACATT CTTCAGCTGA GCAGCAGCCT TCCATTACAA 
AGCCGAGGCC GGGCCCGCCT CCTAGTGGGC AATGATGACG TCCATATCAT CGCCAGGTCT 
GATGACGAGC TGCTGGATGA CTTTTTTCAT GACCAGAGTA CAGCCACTAG CCAAGCAGGA 
ACCCTGTCCA GCATCCCAAC AGCTCTAACC CGATGGACAG AGGAATGCAA AGTTCTTGAT 
GCTGAGAGTA TGCATGACTG TGTTTCAGTG GTTAAAGTGC CCATTGTCAA TCACCTGGAA 
TTCTTGAGAG ATGAGGAGCT AGAAGAGAGA AGGGAGAAAC GCAGGAAACA ATTGGCTGAG 
GAAGAAACAA AAATAATAGA CAAAGGCAAA GAAGACAAGG AGAACAGAGA TCAGAGTGCA 
CAGTGTACTG TAACTAAGAC AAATGACTCC ACTGAACAGA ATGTCTCAGA TGGGACTCCT 
ATGCCTGACA GCTATCCAAC AACCCCCTCT TCAACGGATG CACCTACATC TGAGTCCAAG 
GAAACACTTG GTACCCTACA ACCCTCCCAG CAACAGCCAG CACTTCCACC CCCACCATCC 
TTAGGAGAGA TTCCTCAGGA ACTACAGTCC CCTGCTGAAG AAGTGGCAAA CTCTACACAG 
CTATTGATGC CTATAGAGTT GGAGGAATTG GGTCCCACAA GACCAAGTGG AGAAGCAGAA 
ACAACTCAAA TGGAATTGTC TCCAGCTCCC ACCATAACCT CTCTTTCCCC AGAGAGAGCT 
GAAGATTCAG ACGCGTTAAC AGCTGTCAGC AGTCAGCTGG AAGGTTCTCC CATGGACACC 
AGCAGCCTGG CTTCCTGTAC GTTAGAAGAG GCTGTGGGTG ACACTCCAGC AGCTGGCAGT 
TCTGAGCAGC CCACAGCAGG TAGTTCCACT CCTGGGGATG CGCCATCAGT TGTGGCAGAA 
GTGCAAGGCA GACCTGATGT ATCAAGAGAA TCCAACCAGC CACCTGAGGA CAGCTCCCCC 
CCTGCATCCT CGGAGAGTTC TTCCACTAGA GATTCTGCCG TGGCCATCTC TGGAGCAGAT 
TCTCGTGGAA TTCTAGAAGA GCCACTACCT TCAACAAGCA GTGAAGAAGA AGATCCCCTT 
GCTGGTATCA GTCTCCCTGA AGGTGTGGAT CCCTCCTTTC TGGCTGCTCT CCCTGATGAT 
ATCCGTCGGG AAGTCCTACA GAACCAGCTG GGCATACGTC CACCAACCCG AAGTGCTCCC 
TCCTCAAATA GCTCAGCTCC TGCAGTAGTG GGGAACCCTG GTGTAACTGA AGTGAGCCCT 
GAGTTCCTGG CTGCCCTACC TCCAGCTATT CAAGAGGAAG TGCTAGCACA GCAGAGAGCT 
GAACAGCAGC GACGGGAACT GGCTCAGAAT GCGAGCTCAG ACACCCCCAT GGACCCTGTG 
ACTTTCATTC AGACTTTGCC CTCAGATCTG CGCCGTAGTG TCCTAGAGGA TATGGAAGAC 
AGTGTGTTAG CTGTGATGCC ACCTGACATT GCAGCTGAGG CTCAAGCTCT AAGACGAGAA 
CAAGAAGCCC GACAGCGGCA GCTCATGCAT GAGCGTCTTT TTGGACACAG TAGTACTTCT 
GCACTCTCTG CCATTCTCCG AAGCCCGGCT TTTACCAGTC GCCTGAGTGG CAACCGTGGG 
GTCCAGTATA CTCGCCTTGC TGTACAGAGG GGTGGCACCT TCCAGATGGG GGGTAGCAGC 
AGCCATAATA GGCCTTCTGG CAGTAATGTG GACACACTAC TCCGCCTCCG AGGACGACTC 
CTTCTAGACC ATGAAGCCCT GTCTTGCCTC TTGGTCCTAC TTTTTGTGGA TGAGCCAAAG 
CTCAATACTA GCCGTCTACA CCGAGTACTG AGAAATCTCT GCTATCATGC CCAGACCCGC 
CACTGGGTCA TCCGCAGCCT CCTGTCCATT TTGCAGCGCA GCAGTGAAAG TGAGTTATGC 
ATTGAGACAC CTAAGCTCTC CACAAGTGAA GAAAGGGGCA AGAAATCAAG CAAGAGCTGT 
GCATCAAGCA GTCATGAGAA CCGTCCCCTG GATCTGCTCC ACAAGATGGA GTCCAAAAGT 
TCCAATCAGC TCTCCTGGCT CTCAGTATCA ATGGATGCTG CTTTAGGCTG TAGGACTAAC 
ATATTCCAGA TTCAACGTTC TGGAGGGCGT AAACATACAG AGAAGCATGC AAGCAGTGGT 
TCCACTGTCC ACATCCACCC CCAGGCTGCT CCTGTTGTTT GCAGACATGT TTTGGATACA 
CTCATTCAGT TGGCCAAGGT GTTTCCCAGC CACTTTACAC AGCAGCGGAC CAAAGAAACA 
AACTGTGAGA GTGATCGGGA ACGGGGCAGT AAGCAGGCCT GCAGCCCATG CTCCTCACAG 
TCCTCCAGCA GTGGCATCTG TACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG 
AATGTTAGCC GAAAAGGCAA GAACTCCGTG AAGTCAGTAC CAGTGAGCTC TGGTGGTGAA 
GGGGAAACTT CCCCACACAG CCTTGAAGCC TCTCCACTGG GGCAGCTCAT GAACATGTTG 
TCACATCCAG TCATCCGCCG GAGTTCTCTC TTAACTGAGA AACTCCTGAG ACTCCTCTCT 
CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTATCAGAAG TGCAAACTAA CTCCAGCAAC 
AGTGGTTCCT CCACTGCTGC CACCTCAAAC ACATCTACTA CTACCACCAC CACCACTACT 
GCCACTGCCC CCACGCCCAC ACCCCCAGCT GCAACTACCC CTGTCACTTC TGCTCCAGCT 
CTGGTTGCTG CCACAGCTAT TTCCACCATT ACTGTAGCTG CTTCTACCAC AGTGACTACC 
CCCACAACTG CTACCACTAC TGTTTCAACT TCTACTACTA AAGGCAGCAA ATCTCCAGCA 
AAGGTTGGTG AAGGAGGCAG TGGTATAGAC TTCAAAATGG TGTCCTCTGG CCTCACTGAA 
AACCAGCTAC AGCTCTCTGT AGAGGTGTTG ACATCCCACT CTTGTTCTGA AGAAGGCTTG 
GAAGATGCGG CCAATGTGTT ACTGCAGCTC TCTCGTGGGG ACTCTGGTAC CCGGGACACT 
GTTCTTAAGC TGCTACTAAA TGGAGCCCGC CATCTGGGTT ATACCCTCTG TAAACAGATA 
GGTACCCTAC TCGCTGAACT ACGGGAATAT AATCTGGAGC AGCAGCGGCG AGCCCAATGC 
GAGACCCTCT CTCCTGATGG CCTACCTGAG GAGCAACCAC AGACCACCAA ACTGAAAGGC 
AAAATGCAGA GCAGGTTTGA CATGGCTGAA AATGTGGTAA TTGTGGCATC TCAGAAGCGA 
CCCTTGGGTG GCCGGGAGCT CCAGCTGCCT TCTATGTCCA TGTTGACATC CAAGACATCT 
ACCCAGAAGT TCTTCTTGAG GGTACTACAG GTCATCATCC AGCTGCGGGA TGACACACGA 
CGAGCTAACA AGAAAGCCAA GCAGACAGGC AGGCTAGGTT CCTCCGGTTT AGGCTCAGCT 
AGCAGCATCC AGGCAGCTGT TCGGCAGCTG GAGGCTGAGG CTGATGCCAT TATACAAATG 
GTACGTGAGG GTCAAAGGGC GCGGAGACAG CAACAAGCAG CAACGTCGGA GTCTTCTAAC 
CAGTCAGAGA CATCTGTCCG GAGAGAGGAA TCGCCCATGG ATGTGGACCA GCCATCTCCT 
AGTGCTCAAG ATACTCAGTC CATTGTAATC TCAGATGGAA CACCACAGGG AGAGAAGGAG 
AAAGAGGAGA AACCACCTGA GTTACCCCTG CTCAGTGAGC AACTGAGCCT AGATGAACTG 
TGGGACATGC TAGGGGAATG TTTAAAAGAA CTGGAGGAAT CTCATGACCA GCATGCAGTA 
TTAGTGCTAC AGCCTGCTGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC 
AAGCCTCCTG TCCGAGATAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA TGAACCTCCT 
CCACTCTCCC CCGCCCCCTT AACCCCAGCC ACTCCATCCT CCCTTGACCC ATTCTTCTCC 
AGGGAGCCCT CATCTATGCA CATTTCCTCG AGCCTGCCCC CTGACACACA GAAGTTCCTT 
CGCTTTGCAG AGACTCACCG CACTGTGTTA AACCAGATAC TACGGCAGTC TACCACCCAT 
CTTGCTGATG GGCCTTTTGC TGTCTTGGTA GATTATATCC GTGTCCTCGA CTTTGATGTC 
AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG CGTTTGGACG AAGGGCTCCG GAAAGAAGAT 
ATGGCAGTGC ATGTCCGCCG TGACCATGTA TTTGAAGACT CCTATCGTGA GCTGCATCGC 
AAATCTCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG 
GATGCTGGGG GGCTCCTGCG AGAGTGGTAC ATGATCATCT CTCGAGAGAT GTTCAACCCT 
ATGTATGCCT TGTTCCGTAC CTCACCTGGT GACCGGGTCA CCTACACCAT CAATCCATCT 
TCCCACTGTA ACCCCAACCA CCTCAGCTAC TTCAAGTTTG TTGGACGAAT TGTAGCCAAA 
GCTGTATATG ACAACCGCCT CCTGGAGTGC TACTTTACTA GGTCTTTCTA CAAACACATC 
TTGGGCAAAT CTGTCAGGTA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC 
CTAGTTTATC TGCTTGAAAA TGATGTCTCT ACATTAGGCT ATGACCTCAC CTTCAGCACT 
GAGGTTCAAG AATTTGGAGT ATGTGAAGTT CGTGACCTCA AACCCAATGG AGCCAACATC 
TTGGTAACAG AGGAGAACAA GAAGGAATAT GTACACCTGG TTTGCCAGAT GAGAATGACA 
GGAGCCATCC GCAAACAGCT GGCAGCCTTC TTGGAGGGCT TCTATGAAAT CATTCCAAAG 
CGCCTCATAT CCATCTTTAC TGAGCAGGAA TTAGAGCTGC TCATATCAGG GCTGCCTACC 
ATCGACATTG ATGATCTAAA ATCTAACACT GAGTACCACA AGTACCAATC CAACTCTATT 
CAGATCCAGT GGTTTTGGAG AGCATTGCGT TCCTTTGACC AAGCAGACCG TGCCAAGTTC 
CTCCAGTTTG TCACAGGTAC TTCCAAGGTA CCCCTACAAG GATTTGCTGC CCTTGAAGGT 
ATGAATGGCA TTCAGAAGTT TCAGATTCAT CGAGATGACA GATCCACAGA TCGCCTGCCT 
TCAGCTCACA CATGTTTTAA TCAACTGGAC CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC 
CGCCACATGC TACTATTGGC CATCCAGGAA TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529012.1
CDS	935..14071
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528949.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528949.4

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGAGAAC AGGATGGAAC TGCTGGAAGC 
ATGGATGCAT CTGCCCAGGG TTTATTAGAA GGAATTGAAC TAGATGGTGA CACACTGGCA 
CCCATGGAGA CAGATGAACC TTCTTCTTCA GATTCTAAGG GCAAATCTAA AATCACACCT 
GCCATGGCTG CAAGAATTAA ACAAATCAAG CCGTTGCTGT CAGCTTCTTC CAGATTAGGC 
CGAGCACTTG CTGAGCTCTT TGGACTCCTT GTTAAGCTTT GTGTGGGTTC TCCTGTCCGC 
CAAAGGAGGA GCCATCATGC TGCCAGTACC ACCACTGCAC CAACACCTGC TGCTAGATCA 
ACAGCTTCAG CCCTCACTAA GCTACTCACA AAAGGCTTAT CATGGCAGCC CCCACCATAT 
ACACCCACTC CCCGCTTCAG GCTGACGTTC TTCATCTGTT CAGTTGGATT CACCTCTCCA 
ATGCTCTTTG ATGAGAGAAA GTATCCTTAC CACCTTATGC TGCAGAAATT TCTCTGCTCT 
GGTGGTCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TATCCATGGG AGGAAAAGTT 
CCTGTTTCTG AGGGATTGGA ACATTCAGAT CTACCTGATG GTACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTTGTAGA AAAGATGGTA AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGTTGCCTG CTAAATTGCC TGGAGGAGTC CAGAGCTTTC CACAATTTAG TGCCCTTCGC 
TTCCTTGTAG TAACTCAAAA AGCAGCCTTT ACTTGTATCA AGAACCTGTG GAACCGGAAA 
CCCCTTAAAG TATATGGGGG AAGAATGGCT GAGTCTATGC TGGCCATTTT GTGCCACATT 
CTCCGAGGGG AACCTGTGAT TCGAGAAAGA CTGAGCAAAG AGAAGGAAGG ATCTCGAGGA 
GAAGAAGAAG CAGGCCAGGA AGAAGGTGGC TCACGCAGAG AACCTCAAGT CAACCAGCAA 
CAGCTACAAC AGCTCATGGA CATGGGTTTC ACAAGAGAAC ATGCCATGGA GGCACTGTTG 
AATACCAGTA CGATGGAACA AGCAACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATA 
GGAGGAGTTG TTCGGGATCT TAGCATGTCA GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAAGA GCGTAAAGCT CGAGAAAAAC AAGAAGAGGA AGAAGCTAAA 
TGTCTAGAGA AGTTTCAAGA TGCTGACCCC TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACAATGT TACCAGGCTG CTTCCATCTT CTTGATGAGT TGCCTGATAC AGTGTACCGT 
GTTTGTGATC TGATCATGAC AGCTATCAAA CGTAATGGAG CTGACTATCG TGATATGATT 
CTAAAGCAAG TTGTCAACCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCTCTAACAA CAAGTGACAC AAAAACAGTT TCAGAGTGGA TCAGTCAGAT GGCTACCCTG 
CCACAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CACTACTTTT TGAGGAGTTG 
AAGTTACCTT GTGCCTGGGT GGTTGAATCA AGTGGCATTC TTAATGTCCT AATCAAACTC 
TTAGAAGTGG TTCAACCCTG CCTACAGGCT GCCAAAGAGC AAAAGGAAGT CCAGACCCCA 
AAGTGGATCA CCCCAGTGTT GCTCCTTATT GATTTTTATG AAAAGACAGC CATCTCATCA 
AAGAGAAGAG CCCAAATGAC TAAGTACCTA CAGTCCAACA GCAACAACTG GCGCTGGTTT 
GATGACCGCT CTGGGCGTTG GTGTAGTTAC AGTGCTAGCA ACAACAGCAC CATTGATTCT 
GCCTGGAAAT CTGGAGAAAC AAGTGTAAGA TTCACTGCTG GCCGACGAAG ATACACTGTC 
CAGTTCACTA CAATGGTGCA GGTTAATGAA GAAACAGGAA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTGCCACG ACTGAGTAAG AACTCAAAAA GCAGCAATGG ACAGGAACTA 
GAGAAGACAC TGGAAGAAAG CAAGGAGACC GATATCAAAC ATAAAGAAAA TAAAGGCAAT 
GATATCCCCT TAGCTCTAGA GAGCACAAAC ACTGAAAAAG AGGCAAGCCT GGATGAAACA 
AAAATTGGGG AGATCCTAAT CCAGGGCCTG ACAGAAGATA TGGTGACTGT TCTAATCCGT 
GCCTGTGTGA GCATGCTGGG AGTCCCTGTG GACCCAGACA CTTTGCATGC CACCCTTCGT 
CTCTGTTTAA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACACGAATGA TCTTGAATTT GACCCAGAGC TCAGGTTTCA ATGGGTTTAC CCCCTTAGTC 
ACCCTTCTCT TAAGGCACAT CATTGAGGAT CCTTGTACTC TTCGTCATAC TATGGAAAAG 
GTTGTTCGCT CGGCAGCCAC AAGTGGAGCT GGTAGTACTA CATCTGGTGT TGTATCTGGC 
AGCCTCGGTT CTCGGGAGAT CAACTATATC CTTAGAGTCC TAGGACCAGC TGCCTGCCGC 
AATCCAGACA TATTCACAGA AGTAGCCAAT TGCTGTATCC GCATTGCCCT TCCAGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGTCCTAAT 
GCTGTACAGC TGGTAAAGAC AACACCTTTG AAGCCCTCAT CTCTGCCTGT CATTCCTGAT 
ACTATTAAGG AAGTTATCTA TGATATGCTG AATGCTTTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGACA AATCTGATCC TAAACCTGGG GGAACGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGACGATGT ATACCAGCAG TACCGGTCAC TTACTCGTCA GAGCAGTGAC 
TTTGATACAC AGTCAGGTTT TTCCTTAAAT AGTCAGGTTT TTGCTGCAGA TGGTGCCCCT 
GCTGAAACTT CCACAACTGG AACCTCCCAA GGAGAGGGAG CTTCAACTCC AGAGGAGACT 
CGAGAAGGGA AGAAAGATAA AGAAGGGGAC CGTACATCTG AGGAAGGCAA GCAGAAAAGC 
AAGGGCAGCA AACCTTTAAT GCCTACCTCT ACTATCCTTC GTCTTCTGGC AGAGTTGGTG 
AGGTCCTATG TTGGTATTGC TACCCTGATT GCAAACTACA GCTATACCGT GGGCCAGTCT 
GAGCTGATCA AAGAGGACTG CAGTGTGCTA GCTTTTGTCC TGGACCACCT CCTCCCACAC 
ACCCAGAATG CAGAAGACAA GGATACACCT GCCCTGGCAC GTCTATTCCT TGCAAGTCTG 
GCTGCTGCGG GGAGTGGCAC AGATGCTCAA GTGGCCCTAG TGAATGAAGT GAAAGCAGCC 
CTGGGACGGG CGCTTGCTAT GGCTGAGAGT ACAGAGAAGC ACGCCAGACT TCAGGCAGTG 
ATGTGTATCA TCAGTACTAT CATGGAATCC TGCCCCTCCA CCTCCAGCTT CTACAGTAGT 
GCTACAGCCA AGACTCAGCA TAATGGCATG AACAACATCA TTCGACTGTT CCTAAAGAAG 
GGACTAGTCA ATGACCTGGC CAGAGTTCCT CACAGCCTAG ACCTGTCCAG TCCCAATATG 
GCCAACACAG TCAATGCTGC TCTAAAACCC TTGGAAACAC TTTCCCGGAT TGTAAATCAA 
CCCAGTAGCC TTTTTGGCAG CAAGAGTGCT TCTAGCAAGA ACAAGTCTGA GCAGGATGCT 
CAAGGAGCTT CTCAGGACTC CAGCAGCCAT CAGCAAGATC CAGGCGAGCC TGGGGAGGCA 
GAAGTGCAGG AGGAGGATCA TGATGTCACT CAGACAGAGG TGGCAGATGG GGATATCATG 
GATGGGGAGG CTGAAACCGA CTCAGTGGTG ATTGCCGGGC AGCCTGAGGT GCTCAGTTCA 
CAAGAGATGC AGGTTGAAAA TGAACTGGAG GACCTGATAG ATGAGTTGCT TGAGAGGGAT 
GGCGGATCTG GGAACAGTAC AATTATAGTG AGCAGAAGTG GAGAGGATGA ATCACAAGAG 
GACGTGCTGA TGGATGAAGC TCCTTCCAAC CTCAGCCAAG CTTCCACCTT GCAGGCCAAC 
CGAGAAGATT CCATGAATAT CCTGGACCCT GAGGATGAGG AGGAACATAC TCAGGAAGAG 
GACAGCAGTG GCAGTAACGA GGATGAGGAT GATAGTCAGG ATGAAGAGGA GGAGGAGGAG 
GAAGATGAGG AAGATGATCA GGAGGATGAT GAAGGTGAAG AAGGAGATGA AGATGATGAC 
GACGATGGTT CTGAGATGGA ATTGGATGAG GATTACCCTG ATATGAACGC TTCTCCCTTG 
GTCCGATTTG AGCGCTTTGA CCGGGAGGAT GATCTCATCA TTGAGTTTGA CAACATGTTC 
TCCAGTGCTA CAGACATTCC CCCATCCCCA GGAAATATCC CTACCACCCA CCCATTGATG 
GTACGTCATG CAGATCACAG TTCCCTGACA CTGGGCAGTG GTTCTTCCAC AACTCGTCTT 
ACCCAGGGCA TCGGTCGCAG TCAAAGGACC CTCAGACAGC TCACTGCCAA TACAGGCCAT 
ACCATTCATG TTCACTACCC TGGGAATCGC CAGCCTAACC CTCCTCTTAT ACTCCAGAGG 
TTGCTTGGTC CCTCTGCAGC TGCTGACATT CTTCAGCTGA GCAGCAGCCT TCCATTACAA 
AGCCGAGGCC GGGCCCGCCT CCTAGTGGGC AATGATGACG TCCATATCAT CGCCAGGTCT 
GATGACGAGC TGCTGGATGA CTTTTTTCAT GACCAGAGTA CAGCCACTAG CCAAGCAGGA 
ACCCTGTCCA GCATCCCAAC AGCTCTAACC CGATGGACAG AGGAATGCAA AGTTCTTGAT 
GCTGAGAGTA TGCATGACTG TGTTTCAGTG GTTAAAGTGC CCATTGTCAA TCACCTGGAA 
TTCTTGAGAG ATGAGGAGCT AGAAGAGAGA AGGGAGAAAC GCAGGAAACA ATTGGCTGAG 
GAAGAAACAA AAATAATAGA CAAAGGCAAA GAAGACAAGG AGAACAGAGA TCAGAGTGCA 
CAGTGTACTG TAACTAAGAC AAATGACTCC ACTGAACAGA ATGTCTCAGA TGGGACTCCT 
ATGCCTGACA GCTATCCAAC AACCCCCTCT TCAACGGATG CACCTACATC TGAGTCCAAG 
GAAACACTTG GTACCCTACA ACCCTCCCAG CAACAGCCAG CACTTCCACC CCCACCATCC 
TTAGGAGAGA TTCCTCAGGA ACTACAGTCC CCTGCTGAAG AAGTGGCAAA CTCTACACAG 
CTATTGATGC CTATAGAGTT GGAGGAATTG GGTCCCACAA GACCAAGTGG AGAAGCAGAA 
ACAACTCAAA TGGAATTGTC TCCAGCTCCC ACCATAACCT CTCTTTCCCC AGAGAGAGCT 
GAAGATTCAG ACGCGTTAAC AGCTGTCAGC AGTCAGCTGG AAGGTTCTCC CATGGACACC 
AGCAGCCTGG CTTCCTGTAC GTTAGAAGAG GCTGTGGGTG ACACTCCAGC AGCTGGCAGT 
TCTGAGCAGC CCACAGCAGG TAGTTCCACT CCTGGGGATG CGCCATCAGT TGTGGCAGAA 
GTGCAAGGCA GACCTGATGT ATCAAGAGAA TCCAACCAGC CACCTGAGGA CAGCTCCCCC 
CCTGCATCCT CGGAGAGTTC TTCCACTAGA GATTCTGCCG TGGCCATCTC TGGAGCAGAT 
TCTCGTGGAA TTCTAGAAGA GCCACTACCT TCAACAAGCA GTGAAGAAGA AGATCCCCTT 
GCTGGTATCA GTCTCCCTGA AGGTGTGGAT CCCTCCTTTC TGGCTGCTCT CCCTGATGAT 
ATCCGTCGGG AAGTCCTACA GAACCAGCTG GGCATACGTC CACCAACCCG AAGTGCTCCC 
TCCTCAAATA GCTCAGCTCC TGCAGTAGTG GGGAACCCTG GTGTAACTGA AGTGAGCCCT 
GAGTTCCTGG CTGCCCTACC TCCAGCTATT CAAGAGGAAG TGCTAGCACA GCAGAGAGCT 
GAACAGCAGC GACGGGAACT GGCTCAGAAT GCGAGCTCAG ACACCCCCAT GGACCCTGTG 
ACTTTCATTC AGACTTTGCC CTCAGATCTG CGCCGTAGTG TCCTAGAGGA TATGGAAGAC 
AGTGTGTTAG CTGTGATGCC ACCTGACATT GCAGCTGAGG CTCAAGCTCT AAGACGAGAA 
CAAGAAGCCC GACAGCGGCA GCTCATGCAT GAGCGTCTTT TTGGACACAG TAGTACTTCT 
GCACTCTCTG CCATTCTCCG AAGCCCGGCT TTTACCAGTC GCCTGAGTGG CAACCGTGGG 
GTCCAGTATA CTCGCCTTGC TGTACAGAGG GGTGGCACCT TCCAGATGGG GGGTAGCAGC 
AGCCATAATA GGCCTTCTGG CAGTAATGTG GACACACTAC TCCGCCTCCG AGGACGACTC 
CTTCTAGACC ATGAAGCCCT GTCTTGCCTC TTGGTCCTAC TTTTTGTGGA TGAGCCAAAG 
CTCAATACTA GCCGTCTACA CCGAGTACTG AGAAATCTCT GCTATCATGC CCAGACCCGC 
CACTGGGTCA TCCGCAGCCT CCTGTCCATT TTGCAGCGCA GCAGTGAAAG TGAGTTATGC 
ATTGAGACAC CTAAGCTCTC CACAAGTGAA GAAAGGGGCA AGAAATCAAG CAAGAGCTGT 
GCATCAAGCA GTCATGAGAA CCGTCCCCTG GATCTGCTCC ACAAGATGGA GTCCAAAAGT 
TCCAATCAGC TCTCCTGGCT CTCAGTATCA ATGGATGCTG CTTTAGGCTG TAGGACTAAC 
ATATTCCAGA TTCAACGTTC TGGAGGGCGT AAACATACAG AGAAGCATGC AAGCAGTGGT 
TCCACTGTCC ACATCCACCC CCAGGCTGCT CCTGTTGTTT GCAGACATGT TTTGGATACA 
CTCATTCAGT TGGCCAAGGT GTTTCCCAGC CACTTTACAC AGCAGCGGAC CAAAGAAACA 
AACTGTGAGA GTGATCGGGA ACGGGGCAGT AAGCAGGCCT GCAGCCCATG CTCCTCACAG 
TCCTCCAGCA GTGGCATCTG TACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG 
AATGTTAGCC GAAAAGGCAA GAACTCCGTG AAGTCAGTAC CAGTGAGCTC TGGTGGTGAA 
GGGGAAACTT CCCCACACAG CCTTGAAGCC TCTCCACTGG GGCAGCTCAT GAACATGTTG 
TCACATCCAG TCATCCGCCG GAGTTCTCTC TTAACTGAGA AACTCCTGAG ACTCCTCTCT 
CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTATCAGAAG TGCAAACTAA CTCCAGCAAC 
AGTGGTTCCT CCACTGCTGC CACCTCAAAC ACATCTACTA CTACCACCAC CACCACTACT 
GCCACTGCCC CCACGCCCAC ACCCCCAGCT GCAACTACCC CTGTCACTTC TGCTCCAGCT 
CTGGTTGCTG CCACAGCTAT TTCCACCATT ACTGTAGCTG CTTCTACCAC AGTGACTACC 
CCCACAACTG CTACCACTAC TGTTTCAACT TCTACTACTA AAGGCAGCAA ATCTCCAGCA 
AAGGTTGGTG AAGGAGGCAG TGGTATAGAC TTCAAAATGG TGTCCTCTGG CCTCACTGAA 
AACCAGCTAC AGCTCTCTGT AGAGGTGTTG ACATCCCACT CTTGTTCTGA AGAAGGCTTG 
GAAGATGCGG CCAATGTGTT ACTGCAGCTC TCTCGTGGGG ACTCTGGTAC CCGGGACACT 
GTTCTTAAGC TGCTACTAAA TGGAGCCCGC CATCTGGGTT ATACCCTCTG TAAACAGATA 
GGTACCCTAC TCGCTGAACT ACGGGAATAT AATCTGGAGC AGCAGCGGCG AGCCCAATGC 
GAGACCCTCT CTCCTGATGG CCTACCTGAG GAGCAACCAC AGACCACCAA ACTGAAAGGC 
AAAATGCAGA GCAGGTTTGA CATGGCTGAA AATGTGGTAA TTGTGGCATC TCAGAAGCGA 
CCCTTGGGTG GCCGGGAGCT CCAGCTGCCT TCTATGTCCA TGTTGACATC CAAGACATCT 
ACCCAGAAGT TCTTCTTGAG GGTACTACAG GTCATCATCC AGCTGCGGGA TGACACACGA 
CGAGCTAACA AGAAAGCCAA GCAGACAGGC AGGCTAGGTT CCTCCGGTTT AGGCTCAGCT 
AGCAGCATCC AGGCAGCTGT TCGGCAGCTG GAGGCTGAGG CTGATGCCAT TATACAAATG 
GTACGTGAGG GTCAAAGGGC GCGGAGACAG CAACAAGCAG CAACGTCGGA GTCTTCTAAC 
CAGTCAGAGA CATCTGTCCG GAGAGAGGAA TCGCCCATGG ATGTGGACCA GCCATCTCCT 
AGTGCTCAAG ATACTCAGTC CATTGTAATC TCAGATGGAA CACCACAGGG AGAGAAGGAG 
AAAGAGGAGA AACCACCTGA GTTACCCCTG CTCAGTGAGC AACTGAGCCT AGATGAACTG 
TGGGACATGC TAGGGGAATG TTTAAAAGAA CTGGAGGAAT CTCATGACCA GCATGCAGTA 
TTAGTGCTAC AGCCTGCTGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC 
AAGCCTCCTG TCCGAGATAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA TGAACCTCCT 
CCACTCTCCC CCGCCCCCTT AACCCCAGCC ACTCCATCCT CCCTTGACCC ATTCTTCTCC 
AGGGAGCCCT CATCTATGCA CATTTCCTCG AGCCTGCCCC CTGACACACA GAAGTTCCTT 
CGCTTTGCAG AGACTCACCG CACTGTGTTA AACCAGATAC TACGGCAGTC TACCACCCAT 
CTTGCTGATG GGCCTTTTGC TGTCTTGGTA GATTATATCC GTGTCCTCGA CTTTGATGTC 
AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG CGTTTGGACG AAGGGCTCCG GAAAGAAGAT 
ATGGCAGTGC ATGTCCGCCG TGACCATGTA TTTGAAGACT CCTATCGTGA GCTGCATCGC 
AAATCTCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG 
GATGCTGGGG GGCTCCTGCG AGAGTGGTAC ATGATCATCT CTCGAGAGAT GTTCAACCCT 
ATGTATGCCT TGTTCCGTAC CTCACCTGGT GACCGGGTCA CCTACACCAT CAATCCATCT 
TCCCACTGTA ACCCCAACCA CCTCAGCTAC TTCAAGTTTG TTGGACGAAT TGTAGCCAAA 
GCTGTATATG ACAACCGCCT CCTGGAGTGC TACTTTACTA GGTCTTTCTA CAAACACATC 
TTGGGCAAAT CTGTCAGGTA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC 
CTAGTTTATC TGCTTGAAAA TGATGTCTCT ACATTAGGCT ATGACCTCAC CTTCAGCACT 
GAGGTTCAAG AATTTGGAGT ATGTGAAGTT CGTGACCTCA AACCCAATGG AGCCAACATC 
TTGGTAACAG AGGAGAACAA GAAGGAATAT GTACACCTGG TTTGCCAGAT GAGAATGACA 
GGAGCCATCC GCAAACAGCT GGCAGCCTTC TTGGAGGGCT TCTATGAAAT CATTCCAAAG 
CGCCTCATAT CCATCTTTAC TGAGCAGGAA TTAGAGCTGC TCATATCAGG GCTGCCTACC 
ATCGACATTG ATGATCTAAA ATCTAACACT GAGTACCACA AGTACCAATC CAACTCTATT 
CAGATCCAGT GGTTTTGGAG AGCATTGCGT TCCTTTGACC AAGCAGACCG TGCCAAGTTC 
CTCCAGTTTG TCACAGGTAC TTCCAAGGTA CCCCTACAAG GATTTGCTGC CCTTGAAGGT 
ATGAATGGCA TTCAGAAGTT TCAGATTCAT CGAGATGACA GATCCACAGA TCGCCTGCCT 
TCAGCTCACA CATGTTTTAA TCAACTGGAC CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC 
CGCCACATGC TACTATTGGC CATCCAGGAA TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251455.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107315.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X33, mRNA.

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CloneID	OMu15194
Clone ID Related Accession (Same CDS sequence)	XM_011247858.1 , XM_006528949.4 , XM_030251455.1 , XM_030251456.1 , XM_006528949.3 , NM_021523.4
Accession Version	XM_030251455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGAGAAC AGGATGGAAC TGCTGGAAGC 
ATGGATGCAT CTGCCCAGGG TTTATTAGAA GGAATTGAAC TAGATGGTGA CACACTGGCA 
CCCATGGAGA CAGATGAACC TTCTTCTTCA GATTCTAAGG GCAAATCTAA AATCACACCT 
GCCATGGCTG CAAGAATTAA ACAAATCAAG CCGTTGCTGT CAGCTTCTTC CAGATTAGGC 
CGAGCACTTG CTGAGCTCTT TGGACTCCTT GTTAAGCTTT GTGTGGGTTC TCCTGTCCGC 
CAAAGGAGGA GCCATCATGC TGCCAGTACC ACCACTGCAC CAACACCTGC TGCTAGATCA 
ACAGCTTCAG CCCTCACTAA GCTACTCACA AAAGGCTTAT CATGGCAGCC CCCACCATAT 
ACACCCACTC CCCGCTTCAG GCTGACGTTC TTCATCTGTT CAGTTGGATT CACCTCTCCA 
ATGCTCTTTG ATGAGAGAAA GTATCCTTAC CACCTTATGC TGCAGAAATT TCTCTGCTCT 
GGTGGTCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TATCCATGGG AGGAAAAGTT 
CCTGTTTCTG AGGGATTGGA ACATTCAGAT CTACCTGATG GTACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTTGTAGA AAAGATGGTA AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGTTGCCTG CTAAATTGCC TGGAGGAGTC CAGAGCTTTC CACAATTTAG TGCCCTTCGC 
TTCCTTGTAG TAACTCAAAA AGCAGCCTTT ACTTGTATCA AGAACCTGTG GAACCGGAAA 
CCCCTTAAAG TATATGGGGG AAGAATGGCT GAGTCTATGC TGGCCATTTT GTGCCACATT 
CTCCGAGGGG AACCTGTGAT TCGAGAAAGA CTGAGCAAAG AGAAGGAAGG ATCTCGAGGA 
GAAGAAGAAG CAGGCCAGGA AGAAGGTGGC TCACGCAGAG AACCTCAAGT CAACCAGCAA 
CAGCTACAAC AGCTCATGGA CATGGGTTTC ACAAGAGAAC ATGCCATGGA GGCACTGTTG 
AATACCAGTA CGATGGAACA AGCAACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATA 
GGAGGAGTTG TTCGGGATCT TAGCATGTCA GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAAGA GCGTAAAGCT CGAGAAAAAC AAGAAGAGGA AGAAGCTAAA 
TGTCTAGAGA AGTTTCAAGA TGCTGACCCC TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACAATGT TACCAGGCTG CTTCCATCTT CTTGATGAGT TGCCTGATAC AGTGTACCGT 
GTTTGTGATC TGATCATGAC AGCTATCAAA CGTAATGGAG CTGACTATCG TGATATGATT 
CTAAAGCAAG TTGTCAACCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCTCTAACAA CAAGTGACAC AAAAACAGTT TCAGAGTGGA TCAGTCAGAT GGCTACCCTG 
CCACAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CACTACTTTT TGAGGAGTTG 
AAGTTACCTT GTGCCTGGGT GGTTGAATCA AGTGGCATTC TTAATGTCCT AATCAAACTC 
TTAGAAGTGG TTCAACCCTG CCTACAGGCT GCCAAAGAGC AAAAGGAAGT CCAGACCCCA 
AAGTGGATCA CCCCAGTGTT GCTCCTTATT GATTTTTATG AAAAGACAGC CATCTCATCA 
AAGAGAAGAG CCCAAATGAC TAAGTACCTA CAGTCCAACA GCAACAACTG GCGCTGGTTT 
GATGACCGCT CTGGGCGTTG GTGTAGTTAC AGTGCTAGCA ACAACAGCAC CATTGATTCT 
GCCTGGAAAT CTGGAGAAAC AAGTGTAAGA TTCACTGCTG GCCGACGAAG ATACACTGTC 
CAGTTCACTA CAATGGTGCA GGTTAATGAA GAAACAGGAA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTGCCACG ACTGAGTAAG AACTCAAAAA GCAGCAATGG ACAGGAACTA 
GAGAAGACAC TGGAAGAAAG CAAGGAGACC GATATCAAAC ATAAAGAAAA TAAAGGCAAT 
GATATCCCCT TAGCTCTAGA GAGCACAAAC ACTGAAAAAG AGGCAAGCCT GGATGAAACA 
AAAATTGGGG AGATCCTAAT CCAGGGCCTG ACAGAAGATA TGGTGACTGT TCTAATCCGT 
GCCTGTGTGA GCATGCTGGG AGTCCCTGTG GACCCAGACA CTTTGCATGC CACCCTTCGT 
CTCTGTTTAA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACACGAATGA TCTTGAATTT GACCCAGAGC TCAGGTTTCA ATGGGTTTAC CCCCTTAGTC 
ACCCTTCTCT TAAGGCACAT CATTGAGGAT CCTTGTACTC TTCGTCATAC TATGGAAAAG 
GTTGTTCGCT CGGCAGCCAC AAGTGGAGCT GGTAGTACTA CATCTGGTGT TGTATCTGGC 
AGCCTCGGTT CTCGGGAGAT CAACTATATC CTTAGAGTCC TAGGACCAGC TGCCTGCCGC 
AATCCAGACA TATTCACAGA AGTAGCCAAT TGCTGTATCC GCATTGCCCT TCCAGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGTCCTAAT 
GCTGTACAGC TGGTAAAGAC AACACCTTTG AAGCCCTCAT CTCTGCCTGT CATTCCTGAT 
ACTATTAAGG AAGTTATCTA TGATATGCTG AATGCTTTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGACA AATCTGATCC TAAACCTGGG GGAACGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGACGATGT ATACCAGCAG TACCGGTCAC TTACTCGTCA GAGCAGTGAC 
TTTGATACAC AGTCAGGTTT TTCCTTAAAT AGTCAGGTTT TTGCTGCAGA TGGTGCCCCT 
GCTGAAACTT CCACAACTGG AACCTCCCAA GGAGAGGGAG CTTCAACTCC AGAGGAGACT 
CGAGAAGGGA AGAAAGATAA AGAAGGGGAC CGTACATCTG AGGAAGGCAA GCAGAAAAGC 
AAGGGCAGCA AACCTTTAAT GCCTACCTCT ACTATCCTTC GTCTTCTGGC AGAGTTGGTG 
AGGTCCTATG TTGGTATTGC TACCCTGATT GCAAACTACA GCTATACCGT GGGCCAGTCT 
GAGCTGATCA AAGAGGACTG CAGTGTGCTA GCTTTTGTCC TGGACCACCT CCTCCCACAC 
ACCCAGAATG CAGAAGACAA GGATACACCT GCCCTGGCAC GTCTATTCCT TGCAAGTCTG 
GCTGCTGCGG GGAGTGGCAC AGATGCTCAA GTGGCCCTAG TGAATGAAGT GAAAGCAGCC 
CTGGGACGGG CGCTTGCTAT GGCTGAGAGT ACAGAGAAGC ACGCCAGACT TCAGGCAGTG 
ATGTGTATCA TCAGTACTAT CATGGAATCC TGCCCCTCCA CCTCCAGCTT CTACAGTAGT 
GCTACAGCCA AGACTCAGCA TAATGGCATG AACAACATCA TTCGACTGTT CCTAAAGAAG 
GGACTAGTCA ATGACCTGGC CAGAGTTCCT CACAGCCTAG ACCTGTCCAG TCCCAATATG 
GCCAACACAG TCAATGCTGC TCTAAAACCC TTGGAAACAC TTTCCCGGAT TGTAAATCAA 
CCCAGTAGCC TTTTTGGCAG CAAGAGTGCT TCTAGCAAGA ACAAGTCTGA GCAGGATGCT 
CAAGGAGCTT CTCAGGACTC CAGCAGCCAT CAGCAAGATC CAGGCGAGCC TGGGGAGGCA 
GAAGTGCAGG AGGAGGATCA TGATGTCACT CAGACAGAGG TGGCAGATGG GGATATCATG 
GATGGGGAGG CTGAAACCGA CTCAGTGGTG ATTGCCGGGC AGCCTGAGGT GCTCAGTTCA 
CAAGAGATGC AGGTTGAAAA TGAACTGGAG GACCTGATAG ATGAGTTGCT TGAGAGGGAT 
GGCGGATCTG GGAACAGTAC AATTATAGTG AGCAGAAGTG GAGAGGATGA ATCACAAGAG 
GACGTGCTGA TGGATGAAGC TCCTTCCAAC CTCAGCCAAG CTTCCACCTT GCAGGCCAAC 
CGAGAAGATT CCATGAATAT CCTGGACCCT GAGGATGAGG AGGAACATAC TCAGGAAGAG 
GACAGCAGTG GCAGTAACGA GGATGAGGAT GATAGTCAGG ATGAAGAGGA GGAGGAGGAG 
GAAGATGAGG AAGATGATCA GGAGGATGAT GAAGGTGAAG AAGGAGATGA AGATGATGAC 
GACGATGGTT CTGAGATGGA ATTGGATGAG GATTACCCTG ATATGAACGC TTCTCCCTTG 
GTCCGATTTG AGCGCTTTGA CCGGGAGGAT GATCTCATCA TTGAGTTTGA CAACATGTTC 
TCCAGTGCTA CAGACATTCC CCCATCCCCA GGAAATATCC CTACCACCCA CCCATTGATG 
GTACGTCATG CAGATCACAG TTCCCTGACA CTGGGCAGTG GTTCTTCCAC AACTCGTCTT 
ACCCAGGGCA TCGGTCGCAG TCAAAGGACC CTCAGACAGC TCACTGCCAA TACAGGCCAT 
ACCATTCATG TTCACTACCC TGGGAATCGC CAGCCTAACC CTCCTCTTAT ACTCCAGAGG 
TTGCTTGGTC CCTCTGCAGC TGCTGACATT CTTCAGCTGA GCAGCAGCCT TCCATTACAA 
AGCCGAGGCC GGGCCCGCCT CCTAGTGGGC AATGATGACG TCCATATCAT CGCCAGGTCT 
GATGACGAGC TGCTGGATGA CTTTTTTCAT GACCAGAGTA CAGCCACTAG CCAAGCAGGA 
ACCCTGTCCA GCATCCCAAC AGCTCTAACC CGATGGACAG AGGAATGCAA AGTTCTTGAT 
GCTGAGAGTA TGCATGACTG TGTTTCAGTG GTTAAAGTGC CCATTGTCAA TCACCTGGAA 
TTCTTGAGAG ATGAGGAGCT AGAAGAGAGA AGGGAGAAAC GCAGGAAACA ATTGGCTGAG 
GAAGAAACAA AAATAATAGA CAAAGGCAAA GAAGACAAGG AGAACAGAGA TCAGAGTGCA 
CAGTGTACTG TAACTAAGAC AAATGACTCC ACTGAACAGA ATGTCTCAGA TGGGACTCCT 
ATGCCTGACA GCTATCCAAC AACCCCCTCT TCAACGGATG CACCTACATC TGAGTCCAAG 
GAAACACTTG GTACCCTACA ACCCTCCCAG CAACAGCCAG CACTTCCACC CCCACCATCC 
TTAGGAGAGA TTCCTCAGGA ACTACAGTCC CCTGCTGAAG AAGTGGCAAA CTCTACACAG 
CTATTGATGC CTATAGAGTT GGAGGAATTG GGTCCCACAA GACCAAGTGG AGAAGCAGAA 
ACAACTCAAA TGGAATTGTC TCCAGCTCCC ACCATAACCT CTCTTTCCCC AGAGAGAGCT 
GAAGATTCAG ACGCGTTAAC AGCTGTCAGC AGTCAGCTGG AAGGTTCTCC CATGGACACC 
AGCAGCCTGG CTTCCTGTAC GTTAGAAGAG GCTGTGGGTG ACACTCCAGC AGCTGGCAGT 
TCTGAGCAGC CCACAGCAGG TAGTTCCACT CCTGGGGATG CGCCATCAGT TGTGGCAGAA 
GTGCAAGGCA GACCTGATGT ATCAAGAGAA TCCAACCAGC CACCTGAGGA CAGCTCCCCC 
CCTGCATCCT CGGAGAGTTC TTCCACTAGA GATTCTGCCG TGGCCATCTC TGGAGCAGAT 
TCTCGTGGAA TTCTAGAAGA GCCACTACCT TCAACAAGCA GTGAAGAAGA AGATCCCCTT 
GCTGGTATCA GTCTCCCTGA AGGTGTGGAT CCCTCCTTTC TGGCTGCTCT CCCTGATGAT 
ATCCGTCGGG AAGTCCTACA GAACCAGCTG GGCATACGTC CACCAACCCG AAGTGCTCCC 
TCCTCAAATA GCTCAGCTCC TGCAGTAGTG GGGAACCCTG GTGTAACTGA AGTGAGCCCT 
GAGTTCCTGG CTGCCCTACC TCCAGCTATT CAAGAGGAAG TGCTAGCACA GCAGAGAGCT 
GAACAGCAGC GACGGGAACT GGCTCAGAAT GCGAGCTCAG ACACCCCCAT GGACCCTGTG 
ACTTTCATTC AGACTTTGCC CTCAGATCTG CGCCGTAGTG TCCTAGAGGA TATGGAAGAC 
AGTGTGTTAG CTGTGATGCC ACCTGACATT GCAGCTGAGG CTCAAGCTCT AAGACGAGAA 
CAAGAAGCCC GACAGCGGCA GCTCATGCAT GAGCGTCTTT TTGGACACAG TAGTACTTCT 
GCACTCTCTG CCATTCTCCG AAGCCCGGCT TTTACCAGTC GCCTGAGTGG CAACCGTGGG 
GTCCAGTATA CTCGCCTTGC TGTACAGAGG GGTGGCACCT TCCAGATGGG GGGTAGCAGC 
AGCCATAATA GGCCTTCTGG CAGTAATGTG GACACACTAC TCCGCCTCCG AGGACGACTC 
CTTCTAGACC ATGAAGCCCT GTCTTGCCTC TTGGTCCTAC TTTTTGTGGA TGAGCCAAAG 
CTCAATACTA GCCGTCTACA CCGAGTACTG AGAAATCTCT GCTATCATGC CCAGACCCGC 
CACTGGGTCA TCCGCAGCCT CCTGTCCATT TTGCAGCGCA GCAGTGAAAG TGAGTTATGC 
ATTGAGACAC CTAAGCTCTC CACAAGTGAA GAAAGGGGCA AGAAATCAAG CAAGAGCTGT 
GCATCAAGCA GTCATGAGAA CCGTCCCCTG GATCTGCTCC ACAAGATGGA GTCCAAAAGT 
TCCAATCAGC TCTCCTGGCT CTCAGTATCA ATGGATGCTG CTTTAGGCTG TAGGACTAAC 
ATATTCCAGA TTCAACGTTC TGGAGGGCGT AAACATACAG AGAAGCATGC AAGCAGTGGT 
TCCACTGTCC ACATCCACCC CCAGGCTGCT CCTGTTGTTT GCAGACATGT TTTGGATACA 
CTCATTCAGT TGGCCAAGGT GTTTCCCAGC CACTTTACAC AGCAGCGGAC CAAAGAAACA 
AACTGTGAGA GTGATCGGGA ACGGGGCAGT AAGCAGGCCT GCAGCCCATG CTCCTCACAG 
TCCTCCAGCA GTGGCATCTG TACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG 
AATGTTAGCC GAAAAGGCAA GAACTCCGTG AAGTCAGTAC CAGTGAGCTC TGGTGGTGAA 
GGGGAAACTT CCCCACACAG CCTTGAAGCC TCTCCACTGG GGCAGCTCAT GAACATGTTG 
TCACATCCAG TCATCCGCCG GAGTTCTCTC TTAACTGAGA AACTCCTGAG ACTCCTCTCT 
CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTATCAGAAG TGCAAACTAA CTCCAGCAAC 
AGTGGTTCCT CCACTGCTGC CACCTCAAAC ACATCTACTA CTACCACCAC CACCACTACT 
GCCACTGCCC CCACGCCCAC ACCCCCAGCT GCAACTACCC CTGTCACTTC TGCTCCAGCT 
CTGGTTGCTG CCACAGCTAT TTCCACCATT ACTGTAGCTG CTTCTACCAC AGTGACTACC 
CCCACAACTG CTACCACTAC TGTTTCAACT TCTACTACTA AAGGCAGCAA ATCTCCAGCA 
AAGGTTGGTG AAGGAGGCAG TGGTATAGAC TTCAAAATGG TGTCCTCTGG CCTCACTGAA 
AACCAGCTAC AGCTCTCTGT AGAGGTGTTG ACATCCCACT CTTGTTCTGA AGAAGGCTTG 
GAAGATGCGG CCAATGTGTT ACTGCAGCTC TCTCGTGGGG ACTCTGGTAC CCGGGACACT 
GTTCTTAAGC TGCTACTAAA TGGAGCCCGC CATCTGGGTT ATACCCTCTG TAAACAGATA 
GGTACCCTAC TCGCTGAACT ACGGGAATAT AATCTGGAGC AGCAGCGGCG AGCCCAATGC 
GAGACCCTCT CTCCTGATGG CCTACCTGAG GAGCAACCAC AGACCACCAA ACTGAAAGGC 
AAAATGCAGA GCAGGTTTGA CATGGCTGAA AATGTGGTAA TTGTGGCATC TCAGAAGCGA 
CCCTTGGGTG GCCGGGAGCT CCAGCTGCCT TCTATGTCCA TGTTGACATC CAAGACATCT 
ACCCAGAAGT TCTTCTTGAG GGTACTACAG GTCATCATCC AGCTGCGGGA TGACACACGA 
CGAGCTAACA AGAAAGCCAA GCAGACAGGC AGGCTAGGTT CCTCCGGTTT AGGCTCAGCT 
AGCAGCATCC AGGCAGCTGT TCGGCAGCTG GAGGCTGAGG CTGATGCCAT TATACAAATG 
GTACGTGAGG GTCAAAGGGC GCGGAGACAG CAACAAGCAG CAACGTCGGA GTCTTCTAAC 
CAGTCAGAGA CATCTGTCCG GAGAGAGGAA TCGCCCATGG ATGTGGACCA GCCATCTCCT 
AGTGCTCAAG ATACTCAGTC CATTGTAATC TCAGATGGAA CACCACAGGG AGAGAAGGAG 
AAAGAGGAGA AACCACCTGA GTTACCCCTG CTCAGTGAGC AACTGAGCCT AGATGAACTG 
TGGGACATGC TAGGGGAATG TTTAAAAGAA CTGGAGGAAT CTCATGACCA GCATGCAGTA 
TTAGTGCTAC AGCCTGCTGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC 
AAGCCTCCTG TCCGAGATAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA TGAACCTCCT 
CCACTCTCCC CCGCCCCCTT AACCCCAGCC ACTCCATCCT CCCTTGACCC ATTCTTCTCC 
AGGGAGCCCT CATCTATGCA CATTTCCTCG AGCCTGCCCC CTGACACACA GAAGTTCCTT 
CGCTTTGCAG AGACTCACCG CACTGTGTTA AACCAGATAC TACGGCAGTC TACCACCCAT 
CTTGCTGATG GGCCTTTTGC TGTCTTGGTA GATTATATCC GTGTCCTCGA CTTTGATGTC 
AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG CGTTTGGACG AAGGGCTCCG GAAAGAAGAT 
ATGGCAGTGC ATGTCCGCCG TGACCATGTA TTTGAAGACT CCTATCGTGA GCTGCATCGC 
AAATCTCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG 
GATGCTGGGG GGCTCCTGCG AGAGTGGTAC ATGATCATCT CTCGAGAGAT GTTCAACCCT 
ATGTATGCCT TGTTCCGTAC CTCACCTGGT GACCGGGTCA CCTACACCAT CAATCCATCT 
TCCCACTGTA ACCCCAACCA CCTCAGCTAC TTCAAGTTTG TTGGACGAAT TGTAGCCAAA 
GCTGTATATG ACAACCGCCT CCTGGAGTGC TACTTTACTA GGTCTTTCTA CAAACACATC 
TTGGGCAAAT CTGTCAGGTA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC 
CTAGTTTATC TGCTTGAAAA TGATGTCTCT ACATTAGGCT ATGACCTCAC CTTCAGCACT 
GAGGTTCAAG AATTTGGAGT ATGTGAAGTT CGTGACCTCA AACCCAATGG AGCCAACATC 
TTGGTAACAG AGGAGAACAA GAAGGAATAT GTACACCTGG TTTGCCAGAT GAGAATGACA 
GGAGCCATCC GCAAACAGCT GGCAGCCTTC TTGGAGGGCT TCTATGAAAT CATTCCAAAG 
CGCCTCATAT CCATCTTTAC TGAGCAGGAA TTAGAGCTGC TCATATCAGG GCTGCCTACC 
ATCGACATTG ATGATCTAAA ATCTAACACT GAGTACCACA AGTACCAATC CAACTCTATT 
CAGATCCAGT GGTTTTGGAG AGCATTGCGT TCCTTTGACC AAGCAGACCG TGCCAAGTTC 
CTCCAGTTTG TCACAGGTAC TTCCAAGGTA CCCCTACAAG GATTTGCTGC CCTTGAAGGT 
ATGAATGGCA TTCAGAAGTT TCAGATTCAT CGAGATGACA GATCCACAGA TCGCCTGCCT 
TCAGCTCACA CATGTTTTAA TCAACTGGAC CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC 
CGCCACATGC TACTATTGGC CATCCAGGAA TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107315.1
CDS	1071..14207
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251455.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251455.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGAGAAC AGGATGGAAC TGCTGGAAGC 
ATGGATGCAT CTGCCCAGGG TTTATTAGAA GGAATTGAAC TAGATGGTGA CACACTGGCA 
CCCATGGAGA CAGATGAACC TTCTTCTTCA GATTCTAAGG GCAAATCTAA AATCACACCT 
GCCATGGCTG CAAGAATTAA ACAAATCAAG CCGTTGCTGT CAGCTTCTTC CAGATTAGGC 
CGAGCACTTG CTGAGCTCTT TGGACTCCTT GTTAAGCTTT GTGTGGGTTC TCCTGTCCGC 
CAAAGGAGGA GCCATCATGC TGCCAGTACC ACCACTGCAC CAACACCTGC TGCTAGATCA 
ACAGCTTCAG CCCTCACTAA GCTACTCACA AAAGGCTTAT CATGGCAGCC CCCACCATAT 
ACACCCACTC CCCGCTTCAG GCTGACGTTC TTCATCTGTT CAGTTGGATT CACCTCTCCA 
ATGCTCTTTG ATGAGAGAAA GTATCCTTAC CACCTTATGC TGCAGAAATT TCTCTGCTCT 
GGTGGTCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TATCCATGGG AGGAAAAGTT 
CCTGTTTCTG AGGGATTGGA ACATTCAGAT CTACCTGATG GTACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTTGTAGA AAAGATGGTA AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGTTGCCTG CTAAATTGCC TGGAGGAGTC CAGAGCTTTC CACAATTTAG TGCCCTTCGC 
TTCCTTGTAG TAACTCAAAA AGCAGCCTTT ACTTGTATCA AGAACCTGTG GAACCGGAAA 
CCCCTTAAAG TATATGGGGG AAGAATGGCT GAGTCTATGC TGGCCATTTT GTGCCACATT 
CTCCGAGGGG AACCTGTGAT TCGAGAAAGA CTGAGCAAAG AGAAGGAAGG ATCTCGAGGA 
GAAGAAGAAG CAGGCCAGGA AGAAGGTGGC TCACGCAGAG AACCTCAAGT CAACCAGCAA 
CAGCTACAAC AGCTCATGGA CATGGGTTTC ACAAGAGAAC ATGCCATGGA GGCACTGTTG 
AATACCAGTA CGATGGAACA AGCAACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATA 
GGAGGAGTTG TTCGGGATCT TAGCATGTCA GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAAGA GCGTAAAGCT CGAGAAAAAC AAGAAGAGGA AGAAGCTAAA 
TGTCTAGAGA AGTTTCAAGA TGCTGACCCC TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACAATGT TACCAGGCTG CTTCCATCTT CTTGATGAGT TGCCTGATAC AGTGTACCGT 
GTTTGTGATC TGATCATGAC AGCTATCAAA CGTAATGGAG CTGACTATCG TGATATGATT 
CTAAAGCAAG TTGTCAACCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCTCTAACAA CAAGTGACAC AAAAACAGTT TCAGAGTGGA TCAGTCAGAT GGCTACCCTG 
CCACAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CACTACTTTT TGAGGAGTTG 
AAGTTACCTT GTGCCTGGGT GGTTGAATCA AGTGGCATTC TTAATGTCCT AATCAAACTC 
TTAGAAGTGG TTCAACCCTG CCTACAGGCT GCCAAAGAGC AAAAGGAAGT CCAGACCCCA 
AAGTGGATCA CCCCAGTGTT GCTCCTTATT GATTTTTATG AAAAGACAGC CATCTCATCA 
AAGAGAAGAG CCCAAATGAC TAAGTACCTA CAGTCCAACA GCAACAACTG GCGCTGGTTT 
GATGACCGCT CTGGGCGTTG GTGTAGTTAC AGTGCTAGCA ACAACAGCAC CATTGATTCT 
GCCTGGAAAT CTGGAGAAAC AAGTGTAAGA TTCACTGCTG GCCGACGAAG ATACACTGTC 
CAGTTCACTA CAATGGTGCA GGTTAATGAA GAAACAGGAA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTGCCACG ACTGAGTAAG AACTCAAAAA GCAGCAATGG ACAGGAACTA 
GAGAAGACAC TGGAAGAAAG CAAGGAGACC GATATCAAAC ATAAAGAAAA TAAAGGCAAT 
GATATCCCCT TAGCTCTAGA GAGCACAAAC ACTGAAAAAG AGGCAAGCCT GGATGAAACA 
AAAATTGGGG AGATCCTAAT CCAGGGCCTG ACAGAAGATA TGGTGACTGT TCTAATCCGT 
GCCTGTGTGA GCATGCTGGG AGTCCCTGTG GACCCAGACA CTTTGCATGC CACCCTTCGT 
CTCTGTTTAA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACACGAATGA TCTTGAATTT GACCCAGAGC TCAGGTTTCA ATGGGTTTAC CCCCTTAGTC 
ACCCTTCTCT TAAGGCACAT CATTGAGGAT CCTTGTACTC TTCGTCATAC TATGGAAAAG 
GTTGTTCGCT CGGCAGCCAC AAGTGGAGCT GGTAGTACTA CATCTGGTGT TGTATCTGGC 
AGCCTCGGTT CTCGGGAGAT CAACTATATC CTTAGAGTCC TAGGACCAGC TGCCTGCCGC 
AATCCAGACA TATTCACAGA AGTAGCCAAT TGCTGTATCC GCATTGCCCT TCCAGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGTCCTAAT 
GCTGTACAGC TGGTAAAGAC AACACCTTTG AAGCCCTCAT CTCTGCCTGT CATTCCTGAT 
ACTATTAAGG AAGTTATCTA TGATATGCTG AATGCTTTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGACA AATCTGATCC TAAACCTGGG GGAACGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGACGATGT ATACCAGCAG TACCGGTCAC TTACTCGTCA GAGCAGTGAC 
TTTGATACAC AGTCAGGTTT TTCCTTAAAT AGTCAGGTTT TTGCTGCAGA TGGTGCCCCT 
GCTGAAACTT CCACAACTGG AACCTCCCAA GGAGAGGGAG CTTCAACTCC AGAGGAGACT 
CGAGAAGGGA AGAAAGATAA AGAAGGGGAC CGTACATCTG AGGAAGGCAA GCAGAAAAGC 
AAGGGCAGCA AACCTTTAAT GCCTACCTCT ACTATCCTTC GTCTTCTGGC AGAGTTGGTG 
AGGTCCTATG TTGGTATTGC TACCCTGATT GCAAACTACA GCTATACCGT GGGCCAGTCT 
GAGCTGATCA AAGAGGACTG CAGTGTGCTA GCTTTTGTCC TGGACCACCT CCTCCCACAC 
ACCCAGAATG CAGAAGACAA GGATACACCT GCCCTGGCAC GTCTATTCCT TGCAAGTCTG 
GCTGCTGCGG GGAGTGGCAC AGATGCTCAA GTGGCCCTAG TGAATGAAGT GAAAGCAGCC 
CTGGGACGGG CGCTTGCTAT GGCTGAGAGT ACAGAGAAGC ACGCCAGACT TCAGGCAGTG 
ATGTGTATCA TCAGTACTAT CATGGAATCC TGCCCCTCCA CCTCCAGCTT CTACAGTAGT 
GCTACAGCCA AGACTCAGCA TAATGGCATG AACAACATCA TTCGACTGTT CCTAAAGAAG 
GGACTAGTCA ATGACCTGGC CAGAGTTCCT CACAGCCTAG ACCTGTCCAG TCCCAATATG 
GCCAACACAG TCAATGCTGC TCTAAAACCC TTGGAAACAC TTTCCCGGAT TGTAAATCAA 
CCCAGTAGCC TTTTTGGCAG CAAGAGTGCT TCTAGCAAGA ACAAGTCTGA GCAGGATGCT 
CAAGGAGCTT CTCAGGACTC CAGCAGCCAT CAGCAAGATC CAGGCGAGCC TGGGGAGGCA 
GAAGTGCAGG AGGAGGATCA TGATGTCACT CAGACAGAGG TGGCAGATGG GGATATCATG 
GATGGGGAGG CTGAAACCGA CTCAGTGGTG ATTGCCGGGC AGCCTGAGGT GCTCAGTTCA 
CAAGAGATGC AGGTTGAAAA TGAACTGGAG GACCTGATAG ATGAGTTGCT TGAGAGGGAT 
GGCGGATCTG GGAACAGTAC AATTATAGTG AGCAGAAGTG GAGAGGATGA ATCACAAGAG 
GACGTGCTGA TGGATGAAGC TCCTTCCAAC CTCAGCCAAG CTTCCACCTT GCAGGCCAAC 
CGAGAAGATT CCATGAATAT CCTGGACCCT GAGGATGAGG AGGAACATAC TCAGGAAGAG 
GACAGCAGTG GCAGTAACGA GGATGAGGAT GATAGTCAGG ATGAAGAGGA GGAGGAGGAG 
GAAGATGAGG AAGATGATCA GGAGGATGAT GAAGGTGAAG AAGGAGATGA AGATGATGAC 
GACGATGGTT CTGAGATGGA ATTGGATGAG GATTACCCTG ATATGAACGC TTCTCCCTTG 
GTCCGATTTG AGCGCTTTGA CCGGGAGGAT GATCTCATCA TTGAGTTTGA CAACATGTTC 
TCCAGTGCTA CAGACATTCC CCCATCCCCA GGAAATATCC CTACCACCCA CCCATTGATG 
GTACGTCATG CAGATCACAG TTCCCTGACA CTGGGCAGTG GTTCTTCCAC AACTCGTCTT 
ACCCAGGGCA TCGGTCGCAG TCAAAGGACC CTCAGACAGC TCACTGCCAA TACAGGCCAT 
ACCATTCATG TTCACTACCC TGGGAATCGC CAGCCTAACC CTCCTCTTAT ACTCCAGAGG 
TTGCTTGGTC CCTCTGCAGC TGCTGACATT CTTCAGCTGA GCAGCAGCCT TCCATTACAA 
AGCCGAGGCC GGGCCCGCCT CCTAGTGGGC AATGATGACG TCCATATCAT CGCCAGGTCT 
GATGACGAGC TGCTGGATGA CTTTTTTCAT GACCAGAGTA CAGCCACTAG CCAAGCAGGA 
ACCCTGTCCA GCATCCCAAC AGCTCTAACC CGATGGACAG AGGAATGCAA AGTTCTTGAT 
GCTGAGAGTA TGCATGACTG TGTTTCAGTG GTTAAAGTGC CCATTGTCAA TCACCTGGAA 
TTCTTGAGAG ATGAGGAGCT AGAAGAGAGA AGGGAGAAAC GCAGGAAACA ATTGGCTGAG 
GAAGAAACAA AAATAATAGA CAAAGGCAAA GAAGACAAGG AGAACAGAGA TCAGAGTGCA 
CAGTGTACTG TAACTAAGAC AAATGACTCC ACTGAACAGA ATGTCTCAGA TGGGACTCCT 
ATGCCTGACA GCTATCCAAC AACCCCCTCT TCAACGGATG CACCTACATC TGAGTCCAAG 
GAAACACTTG GTACCCTACA ACCCTCCCAG CAACAGCCAG CACTTCCACC CCCACCATCC 
TTAGGAGAGA TTCCTCAGGA ACTACAGTCC CCTGCTGAAG AAGTGGCAAA CTCTACACAG 
CTATTGATGC CTATAGAGTT GGAGGAATTG GGTCCCACAA GACCAAGTGG AGAAGCAGAA 
ACAACTCAAA TGGAATTGTC TCCAGCTCCC ACCATAACCT CTCTTTCCCC AGAGAGAGCT 
GAAGATTCAG ACGCGTTAAC AGCTGTCAGC AGTCAGCTGG AAGGTTCTCC CATGGACACC 
AGCAGCCTGG CTTCCTGTAC GTTAGAAGAG GCTGTGGGTG ACACTCCAGC AGCTGGCAGT 
TCTGAGCAGC CCACAGCAGG TAGTTCCACT CCTGGGGATG CGCCATCAGT TGTGGCAGAA 
GTGCAAGGCA GACCTGATGT ATCAAGAGAA TCCAACCAGC CACCTGAGGA CAGCTCCCCC 
CCTGCATCCT CGGAGAGTTC TTCCACTAGA GATTCTGCCG TGGCCATCTC TGGAGCAGAT 
TCTCGTGGAA TTCTAGAAGA GCCACTACCT TCAACAAGCA GTGAAGAAGA AGATCCCCTT 
GCTGGTATCA GTCTCCCTGA AGGTGTGGAT CCCTCCTTTC TGGCTGCTCT CCCTGATGAT 
ATCCGTCGGG AAGTCCTACA GAACCAGCTG GGCATACGTC CACCAACCCG AAGTGCTCCC 
TCCTCAAATA GCTCAGCTCC TGCAGTAGTG GGGAACCCTG GTGTAACTGA AGTGAGCCCT 
GAGTTCCTGG CTGCCCTACC TCCAGCTATT CAAGAGGAAG TGCTAGCACA GCAGAGAGCT 
GAACAGCAGC GACGGGAACT GGCTCAGAAT GCGAGCTCAG ACACCCCCAT GGACCCTGTG 
ACTTTCATTC AGACTTTGCC CTCAGATCTG CGCCGTAGTG TCCTAGAGGA TATGGAAGAC 
AGTGTGTTAG CTGTGATGCC ACCTGACATT GCAGCTGAGG CTCAAGCTCT AAGACGAGAA 
CAAGAAGCCC GACAGCGGCA GCTCATGCAT GAGCGTCTTT TTGGACACAG TAGTACTTCT 
GCACTCTCTG CCATTCTCCG AAGCCCGGCT TTTACCAGTC GCCTGAGTGG CAACCGTGGG 
GTCCAGTATA CTCGCCTTGC TGTACAGAGG GGTGGCACCT TCCAGATGGG GGGTAGCAGC 
AGCCATAATA GGCCTTCTGG CAGTAATGTG GACACACTAC TCCGCCTCCG AGGACGACTC 
CTTCTAGACC ATGAAGCCCT GTCTTGCCTC TTGGTCCTAC TTTTTGTGGA TGAGCCAAAG 
CTCAATACTA GCCGTCTACA CCGAGTACTG AGAAATCTCT GCTATCATGC CCAGACCCGC 
CACTGGGTCA TCCGCAGCCT CCTGTCCATT TTGCAGCGCA GCAGTGAAAG TGAGTTATGC 
ATTGAGACAC CTAAGCTCTC CACAAGTGAA GAAAGGGGCA AGAAATCAAG CAAGAGCTGT 
GCATCAAGCA GTCATGAGAA CCGTCCCCTG GATCTGCTCC ACAAGATGGA GTCCAAAAGT 
TCCAATCAGC TCTCCTGGCT CTCAGTATCA ATGGATGCTG CTTTAGGCTG TAGGACTAAC 
ATATTCCAGA TTCAACGTTC TGGAGGGCGT AAACATACAG AGAAGCATGC AAGCAGTGGT 
TCCACTGTCC ACATCCACCC CCAGGCTGCT CCTGTTGTTT GCAGACATGT TTTGGATACA 
CTCATTCAGT TGGCCAAGGT GTTTCCCAGC CACTTTACAC AGCAGCGGAC CAAAGAAACA 
AACTGTGAGA GTGATCGGGA ACGGGGCAGT AAGCAGGCCT GCAGCCCATG CTCCTCACAG 
TCCTCCAGCA GTGGCATCTG TACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG 
AATGTTAGCC GAAAAGGCAA GAACTCCGTG AAGTCAGTAC CAGTGAGCTC TGGTGGTGAA 
GGGGAAACTT CCCCACACAG CCTTGAAGCC TCTCCACTGG GGCAGCTCAT GAACATGTTG 
TCACATCCAG TCATCCGCCG GAGTTCTCTC TTAACTGAGA AACTCCTGAG ACTCCTCTCT 
CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTATCAGAAG TGCAAACTAA CTCCAGCAAC 
AGTGGTTCCT CCACTGCTGC CACCTCAAAC ACATCTACTA CTACCACCAC CACCACTACT 
GCCACTGCCC CCACGCCCAC ACCCCCAGCT GCAACTACCC CTGTCACTTC TGCTCCAGCT 
CTGGTTGCTG CCACAGCTAT TTCCACCATT ACTGTAGCTG CTTCTACCAC AGTGACTACC 
CCCACAACTG CTACCACTAC TGTTTCAACT TCTACTACTA AAGGCAGCAA ATCTCCAGCA 
AAGGTTGGTG AAGGAGGCAG TGGTATAGAC TTCAAAATGG TGTCCTCTGG CCTCACTGAA 
AACCAGCTAC AGCTCTCTGT AGAGGTGTTG ACATCCCACT CTTGTTCTGA AGAAGGCTTG 
GAAGATGCGG CCAATGTGTT ACTGCAGCTC TCTCGTGGGG ACTCTGGTAC CCGGGACACT 
GTTCTTAAGC TGCTACTAAA TGGAGCCCGC CATCTGGGTT ATACCCTCTG TAAACAGATA 
GGTACCCTAC TCGCTGAACT ACGGGAATAT AATCTGGAGC AGCAGCGGCG AGCCCAATGC 
GAGACCCTCT CTCCTGATGG CCTACCTGAG GAGCAACCAC AGACCACCAA ACTGAAAGGC 
AAAATGCAGA GCAGGTTTGA CATGGCTGAA AATGTGGTAA TTGTGGCATC TCAGAAGCGA 
CCCTTGGGTG GCCGGGAGCT CCAGCTGCCT TCTATGTCCA TGTTGACATC CAAGACATCT 
ACCCAGAAGT TCTTCTTGAG GGTACTACAG GTCATCATCC AGCTGCGGGA TGACACACGA 
CGAGCTAACA AGAAAGCCAA GCAGACAGGC AGGCTAGGTT CCTCCGGTTT AGGCTCAGCT 
AGCAGCATCC AGGCAGCTGT TCGGCAGCTG GAGGCTGAGG CTGATGCCAT TATACAAATG 
GTACGTGAGG GTCAAAGGGC GCGGAGACAG CAACAAGCAG CAACGTCGGA GTCTTCTAAC 
CAGTCAGAGA CATCTGTCCG GAGAGAGGAA TCGCCCATGG ATGTGGACCA GCCATCTCCT 
AGTGCTCAAG ATACTCAGTC CATTGTAATC TCAGATGGAA CACCACAGGG AGAGAAGGAG 
AAAGAGGAGA AACCACCTGA GTTACCCCTG CTCAGTGAGC AACTGAGCCT AGATGAACTG 
TGGGACATGC TAGGGGAATG TTTAAAAGAA CTGGAGGAAT CTCATGACCA GCATGCAGTA 
TTAGTGCTAC AGCCTGCTGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC 
AAGCCTCCTG TCCGAGATAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA TGAACCTCCT 
CCACTCTCCC CCGCCCCCTT AACCCCAGCC ACTCCATCCT CCCTTGACCC ATTCTTCTCC 
AGGGAGCCCT CATCTATGCA CATTTCCTCG AGCCTGCCCC CTGACACACA GAAGTTCCTT 
CGCTTTGCAG AGACTCACCG CACTGTGTTA AACCAGATAC TACGGCAGTC TACCACCCAT 
CTTGCTGATG GGCCTTTTGC TGTCTTGGTA GATTATATCC GTGTCCTCGA CTTTGATGTC 
AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG CGTTTGGACG AAGGGCTCCG GAAAGAAGAT 
ATGGCAGTGC ATGTCCGCCG TGACCATGTA TTTGAAGACT CCTATCGTGA GCTGCATCGC 
AAATCTCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG 
GATGCTGGGG GGCTCCTGCG AGAGTGGTAC ATGATCATCT CTCGAGAGAT GTTCAACCCT 
ATGTATGCCT TGTTCCGTAC CTCACCTGGT GACCGGGTCA CCTACACCAT CAATCCATCT 
TCCCACTGTA ACCCCAACCA CCTCAGCTAC TTCAAGTTTG TTGGACGAAT TGTAGCCAAA 
GCTGTATATG ACAACCGCCT CCTGGAGTGC TACTTTACTA GGTCTTTCTA CAAACACATC 
TTGGGCAAAT CTGTCAGGTA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC 
CTAGTTTATC TGCTTGAAAA TGATGTCTCT ACATTAGGCT ATGACCTCAC CTTCAGCACT 
GAGGTTCAAG AATTTGGAGT ATGTGAAGTT CGTGACCTCA AACCCAATGG AGCCAACATC 
TTGGTAACAG AGGAGAACAA GAAGGAATAT GTACACCTGG TTTGCCAGAT GAGAATGACA 
GGAGCCATCC GCAAACAGCT GGCAGCCTTC TTGGAGGGCT TCTATGAAAT CATTCCAAAG 
CGCCTCATAT CCATCTTTAC TGAGCAGGAA TTAGAGCTGC TCATATCAGG GCTGCCTACC 
ATCGACATTG ATGATCTAAA ATCTAACACT GAGTACCACA AGTACCAATC CAACTCTATT 
CAGATCCAGT GGTTTTGGAG AGCATTGCGT TCCTTTGACC AAGCAGACCG TGCCAAGTTC 
CTCCAGTTTG TCACAGGTAC TTCCAAGGTA CCCCTACAAG GATTTGCTGC CCTTGAAGGT 
ATGAATGGCA TTCAGAAGTT TCAGATTCAT CGAGATGACA GATCCACAGA TCGCCTGCCT 
TCAGCTCACA CATGTTTTAA TCAACTGGAC CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC 
CGCCACATGC TACTATTGGC CATCCAGGAA TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251456.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107316.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X34, mRNA.

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CloneID	OMu15194
Clone ID Related Accession (Same CDS sequence)	XM_011247858.1 , XM_006528949.4 , XM_030251455.1 , XM_030251456.1 , XM_006528949.3 , NM_021523.4
Accession Version	XM_030251456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGAGAAC AGGATGGAAC TGCTGGAAGC 
ATGGATGCAT CTGCCCAGGG TTTATTAGAA GGAATTGAAC TAGATGGTGA CACACTGGCA 
CCCATGGAGA CAGATGAACC TTCTTCTTCA GATTCTAAGG GCAAATCTAA AATCACACCT 
GCCATGGCTG CAAGAATTAA ACAAATCAAG CCGTTGCTGT CAGCTTCTTC CAGATTAGGC 
CGAGCACTTG CTGAGCTCTT TGGACTCCTT GTTAAGCTTT GTGTGGGTTC TCCTGTCCGC 
CAAAGGAGGA GCCATCATGC TGCCAGTACC ACCACTGCAC CAACACCTGC TGCTAGATCA 
ACAGCTTCAG CCCTCACTAA GCTACTCACA AAAGGCTTAT CATGGCAGCC CCCACCATAT 
ACACCCACTC CCCGCTTCAG GCTGACGTTC TTCATCTGTT CAGTTGGATT CACCTCTCCA 
ATGCTCTTTG ATGAGAGAAA GTATCCTTAC CACCTTATGC TGCAGAAATT TCTCTGCTCT 
GGTGGTCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TATCCATGGG AGGAAAAGTT 
CCTGTTTCTG AGGGATTGGA ACATTCAGAT CTACCTGATG GTACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTTGTAGA AAAGATGGTA AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGTTGCCTG CTAAATTGCC TGGAGGAGTC CAGAGCTTTC CACAATTTAG TGCCCTTCGC 
TTCCTTGTAG TAACTCAAAA AGCAGCCTTT ACTTGTATCA AGAACCTGTG GAACCGGAAA 
CCCCTTAAAG TATATGGGGG AAGAATGGCT GAGTCTATGC TGGCCATTTT GTGCCACATT 
CTCCGAGGGG AACCTGTGAT TCGAGAAAGA CTGAGCAAAG AGAAGGAAGG ATCTCGAGGA 
GAAGAAGAAG CAGGCCAGGA AGAAGGTGGC TCACGCAGAG AACCTCAAGT CAACCAGCAA 
CAGCTACAAC AGCTCATGGA CATGGGTTTC ACAAGAGAAC ATGCCATGGA GGCACTGTTG 
AATACCAGTA CGATGGAACA AGCAACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATA 
GGAGGAGTTG TTCGGGATCT TAGCATGTCA GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAAGA GCGTAAAGCT CGAGAAAAAC AAGAAGAGGA AGAAGCTAAA 
TGTCTAGAGA AGTTTCAAGA TGCTGACCCC TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACAATGT TACCAGGCTG CTTCCATCTT CTTGATGAGT TGCCTGATAC AGTGTACCGT 
GTTTGTGATC TGATCATGAC AGCTATCAAA CGTAATGGAG CTGACTATCG TGATATGATT 
CTAAAGCAAG TTGTCAACCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCTCTAACAA CAAGTGACAC AAAAACAGTT TCAGAGTGGA TCAGTCAGAT GGCTACCCTG 
CCACAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CACTACTTTT TGAGGAGTTG 
AAGTTACCTT GTGCCTGGGT GGTTGAATCA AGTGGCATTC TTAATGTCCT AATCAAACTC 
TTAGAAGTGG TTCAACCCTG CCTACAGGCT GCCAAAGAGC AAAAGGAAGT CCAGACCCCA 
AAGTGGATCA CCCCAGTGTT GCTCCTTATT GATTTTTATG AAAAGACAGC CATCTCATCA 
AAGAGAAGAG CCCAAATGAC TAAGTACCTA CAGTCCAACA GCAACAACTG GCGCTGGTTT 
GATGACCGCT CTGGGCGTTG GTGTAGTTAC AGTGCTAGCA ACAACAGCAC CATTGATTCT 
GCCTGGAAAT CTGGAGAAAC AAGTGTAAGA TTCACTGCTG GCCGACGAAG ATACACTGTC 
CAGTTCACTA CAATGGTGCA GGTTAATGAA GAAACAGGAA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTGCCACG ACTGAGTAAG AACTCAAAAA GCAGCAATGG ACAGGAACTA 
GAGAAGACAC TGGAAGAAAG CAAGGAGACC GATATCAAAC ATAAAGAAAA TAAAGGCAAT 
GATATCCCCT TAGCTCTAGA GAGCACAAAC ACTGAAAAAG AGGCAAGCCT GGATGAAACA 
AAAATTGGGG AGATCCTAAT CCAGGGCCTG ACAGAAGATA TGGTGACTGT TCTAATCCGT 
GCCTGTGTGA GCATGCTGGG AGTCCCTGTG GACCCAGACA CTTTGCATGC CACCCTTCGT 
CTCTGTTTAA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACACGAATGA TCTTGAATTT GACCCAGAGC TCAGGTTTCA ATGGGTTTAC CCCCTTAGTC 
ACCCTTCTCT TAAGGCACAT CATTGAGGAT CCTTGTACTC TTCGTCATAC TATGGAAAAG 
GTTGTTCGCT CGGCAGCCAC AAGTGGAGCT GGTAGTACTA CATCTGGTGT TGTATCTGGC 
AGCCTCGGTT CTCGGGAGAT CAACTATATC CTTAGAGTCC TAGGACCAGC TGCCTGCCGC 
AATCCAGACA TATTCACAGA AGTAGCCAAT TGCTGTATCC GCATTGCCCT TCCAGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGTCCTAAT 
GCTGTACAGC TGGTAAAGAC AACACCTTTG AAGCCCTCAT CTCTGCCTGT CATTCCTGAT 
ACTATTAAGG AAGTTATCTA TGATATGCTG AATGCTTTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGACA AATCTGATCC TAAACCTGGG GGAACGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGACGATGT ATACCAGCAG TACCGGTCAC TTACTCGTCA GAGCAGTGAC 
TTTGATACAC AGTCAGGTTT TTCCTTAAAT AGTCAGGTTT TTGCTGCAGA TGGTGCCCCT 
GCTGAAACTT CCACAACTGG AACCTCCCAA GGAGAGGGAG CTTCAACTCC AGAGGAGACT 
CGAGAAGGGA AGAAAGATAA AGAAGGGGAC CGTACATCTG AGGAAGGCAA GCAGAAAAGC 
AAGGGCAGCA AACCTTTAAT GCCTACCTCT ACTATCCTTC GTCTTCTGGC AGAGTTGGTG 
AGGTCCTATG TTGGTATTGC TACCCTGATT GCAAACTACA GCTATACCGT GGGCCAGTCT 
GAGCTGATCA AAGAGGACTG CAGTGTGCTA GCTTTTGTCC TGGACCACCT CCTCCCACAC 
ACCCAGAATG CAGAAGACAA GGATACACCT GCCCTGGCAC GTCTATTCCT TGCAAGTCTG 
GCTGCTGCGG GGAGTGGCAC AGATGCTCAA GTGGCCCTAG TGAATGAAGT GAAAGCAGCC 
CTGGGACGGG CGCTTGCTAT GGCTGAGAGT ACAGAGAAGC ACGCCAGACT TCAGGCAGTG 
ATGTGTATCA TCAGTACTAT CATGGAATCC TGCCCCTCCA CCTCCAGCTT CTACAGTAGT 
GCTACAGCCA AGACTCAGCA TAATGGCATG AACAACATCA TTCGACTGTT CCTAAAGAAG 
GGACTAGTCA ATGACCTGGC CAGAGTTCCT CACAGCCTAG ACCTGTCCAG TCCCAATATG 
GCCAACACAG TCAATGCTGC TCTAAAACCC TTGGAAACAC TTTCCCGGAT TGTAAATCAA 
CCCAGTAGCC TTTTTGGCAG CAAGAGTGCT TCTAGCAAGA ACAAGTCTGA GCAGGATGCT 
CAAGGAGCTT CTCAGGACTC CAGCAGCCAT CAGCAAGATC CAGGCGAGCC TGGGGAGGCA 
GAAGTGCAGG AGGAGGATCA TGATGTCACT CAGACAGAGG TGGCAGATGG GGATATCATG 
GATGGGGAGG CTGAAACCGA CTCAGTGGTG ATTGCCGGGC AGCCTGAGGT GCTCAGTTCA 
CAAGAGATGC AGGTTGAAAA TGAACTGGAG GACCTGATAG ATGAGTTGCT TGAGAGGGAT 
GGCGGATCTG GGAACAGTAC AATTATAGTG AGCAGAAGTG GAGAGGATGA ATCACAAGAG 
GACGTGCTGA TGGATGAAGC TCCTTCCAAC CTCAGCCAAG CTTCCACCTT GCAGGCCAAC 
CGAGAAGATT CCATGAATAT CCTGGACCCT GAGGATGAGG AGGAACATAC TCAGGAAGAG 
GACAGCAGTG GCAGTAACGA GGATGAGGAT GATAGTCAGG ATGAAGAGGA GGAGGAGGAG 
GAAGATGAGG AAGATGATCA GGAGGATGAT GAAGGTGAAG AAGGAGATGA AGATGATGAC 
GACGATGGTT CTGAGATGGA ATTGGATGAG GATTACCCTG ATATGAACGC TTCTCCCTTG 
GTCCGATTTG AGCGCTTTGA CCGGGAGGAT GATCTCATCA TTGAGTTTGA CAACATGTTC 
TCCAGTGCTA CAGACATTCC CCCATCCCCA GGAAATATCC CTACCACCCA CCCATTGATG 
GTACGTCATG CAGATCACAG TTCCCTGACA CTGGGCAGTG GTTCTTCCAC AACTCGTCTT 
ACCCAGGGCA TCGGTCGCAG TCAAAGGACC CTCAGACAGC TCACTGCCAA TACAGGCCAT 
ACCATTCATG TTCACTACCC TGGGAATCGC CAGCCTAACC CTCCTCTTAT ACTCCAGAGG 
TTGCTTGGTC CCTCTGCAGC TGCTGACATT CTTCAGCTGA GCAGCAGCCT TCCATTACAA 
AGCCGAGGCC GGGCCCGCCT CCTAGTGGGC AATGATGACG TCCATATCAT CGCCAGGTCT 
GATGACGAGC TGCTGGATGA CTTTTTTCAT GACCAGAGTA CAGCCACTAG CCAAGCAGGA 
ACCCTGTCCA GCATCCCAAC AGCTCTAACC CGATGGACAG AGGAATGCAA AGTTCTTGAT 
GCTGAGAGTA TGCATGACTG TGTTTCAGTG GTTAAAGTGC CCATTGTCAA TCACCTGGAA 
TTCTTGAGAG ATGAGGAGCT AGAAGAGAGA AGGGAGAAAC GCAGGAAACA ATTGGCTGAG 
GAAGAAACAA AAATAATAGA CAAAGGCAAA GAAGACAAGG AGAACAGAGA TCAGAGTGCA 
CAGTGTACTG TAACTAAGAC AAATGACTCC ACTGAACAGA ATGTCTCAGA TGGGACTCCT 
ATGCCTGACA GCTATCCAAC AACCCCCTCT TCAACGGATG CACCTACATC TGAGTCCAAG 
GAAACACTTG GTACCCTACA ACCCTCCCAG CAACAGCCAG CACTTCCACC CCCACCATCC 
TTAGGAGAGA TTCCTCAGGA ACTACAGTCC CCTGCTGAAG AAGTGGCAAA CTCTACACAG 
CTATTGATGC CTATAGAGTT GGAGGAATTG GGTCCCACAA GACCAAGTGG AGAAGCAGAA 
ACAACTCAAA TGGAATTGTC TCCAGCTCCC ACCATAACCT CTCTTTCCCC AGAGAGAGCT 
GAAGATTCAG ACGCGTTAAC AGCTGTCAGC AGTCAGCTGG AAGGTTCTCC CATGGACACC 
AGCAGCCTGG CTTCCTGTAC GTTAGAAGAG GCTGTGGGTG ACACTCCAGC AGCTGGCAGT 
TCTGAGCAGC CCACAGCAGG TAGTTCCACT CCTGGGGATG CGCCATCAGT TGTGGCAGAA 
GTGCAAGGCA GACCTGATGT ATCAAGAGAA TCCAACCAGC CACCTGAGGA CAGCTCCCCC 
CCTGCATCCT CGGAGAGTTC TTCCACTAGA GATTCTGCCG TGGCCATCTC TGGAGCAGAT 
TCTCGTGGAA TTCTAGAAGA GCCACTACCT TCAACAAGCA GTGAAGAAGA AGATCCCCTT 
GCTGGTATCA GTCTCCCTGA AGGTGTGGAT CCCTCCTTTC TGGCTGCTCT CCCTGATGAT 
ATCCGTCGGG AAGTCCTACA GAACCAGCTG GGCATACGTC CACCAACCCG AAGTGCTCCC 
TCCTCAAATA GCTCAGCTCC TGCAGTAGTG GGGAACCCTG GTGTAACTGA AGTGAGCCCT 
GAGTTCCTGG CTGCCCTACC TCCAGCTATT CAAGAGGAAG TGCTAGCACA GCAGAGAGCT 
GAACAGCAGC GACGGGAACT GGCTCAGAAT GCGAGCTCAG ACACCCCCAT GGACCCTGTG 
ACTTTCATTC AGACTTTGCC CTCAGATCTG CGCCGTAGTG TCCTAGAGGA TATGGAAGAC 
AGTGTGTTAG CTGTGATGCC ACCTGACATT GCAGCTGAGG CTCAAGCTCT AAGACGAGAA 
CAAGAAGCCC GACAGCGGCA GCTCATGCAT GAGCGTCTTT TTGGACACAG TAGTACTTCT 
GCACTCTCTG CCATTCTCCG AAGCCCGGCT TTTACCAGTC GCCTGAGTGG CAACCGTGGG 
GTCCAGTATA CTCGCCTTGC TGTACAGAGG GGTGGCACCT TCCAGATGGG GGGTAGCAGC 
AGCCATAATA GGCCTTCTGG CAGTAATGTG GACACACTAC TCCGCCTCCG AGGACGACTC 
CTTCTAGACC ATGAAGCCCT GTCTTGCCTC TTGGTCCTAC TTTTTGTGGA TGAGCCAAAG 
CTCAATACTA GCCGTCTACA CCGAGTACTG AGAAATCTCT GCTATCATGC CCAGACCCGC 
CACTGGGTCA TCCGCAGCCT CCTGTCCATT TTGCAGCGCA GCAGTGAAAG TGAGTTATGC 
ATTGAGACAC CTAAGCTCTC CACAAGTGAA GAAAGGGGCA AGAAATCAAG CAAGAGCTGT 
GCATCAAGCA GTCATGAGAA CCGTCCCCTG GATCTGCTCC ACAAGATGGA GTCCAAAAGT 
TCCAATCAGC TCTCCTGGCT CTCAGTATCA ATGGATGCTG CTTTAGGCTG TAGGACTAAC 
ATATTCCAGA TTCAACGTTC TGGAGGGCGT AAACATACAG AGAAGCATGC AAGCAGTGGT 
TCCACTGTCC ACATCCACCC CCAGGCTGCT CCTGTTGTTT GCAGACATGT TTTGGATACA 
CTCATTCAGT TGGCCAAGGT GTTTCCCAGC CACTTTACAC AGCAGCGGAC CAAAGAAACA 
AACTGTGAGA GTGATCGGGA ACGGGGCAGT AAGCAGGCCT GCAGCCCATG CTCCTCACAG 
TCCTCCAGCA GTGGCATCTG TACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG 
AATGTTAGCC GAAAAGGCAA GAACTCCGTG AAGTCAGTAC CAGTGAGCTC TGGTGGTGAA 
GGGGAAACTT CCCCACACAG CCTTGAAGCC TCTCCACTGG GGCAGCTCAT GAACATGTTG 
TCACATCCAG TCATCCGCCG GAGTTCTCTC TTAACTGAGA AACTCCTGAG ACTCCTCTCT 
CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTATCAGAAG TGCAAACTAA CTCCAGCAAC 
AGTGGTTCCT CCACTGCTGC CACCTCAAAC ACATCTACTA CTACCACCAC CACCACTACT 
GCCACTGCCC CCACGCCCAC ACCCCCAGCT GCAACTACCC CTGTCACTTC TGCTCCAGCT 
CTGGTTGCTG CCACAGCTAT TTCCACCATT ACTGTAGCTG CTTCTACCAC AGTGACTACC 
CCCACAACTG CTACCACTAC TGTTTCAACT TCTACTACTA AAGGCAGCAA ATCTCCAGCA 
AAGGTTGGTG AAGGAGGCAG TGGTATAGAC TTCAAAATGG TGTCCTCTGG CCTCACTGAA 
AACCAGCTAC AGCTCTCTGT AGAGGTGTTG ACATCCCACT CTTGTTCTGA AGAAGGCTTG 
GAAGATGCGG CCAATGTGTT ACTGCAGCTC TCTCGTGGGG ACTCTGGTAC CCGGGACACT 
GTTCTTAAGC TGCTACTAAA TGGAGCCCGC CATCTGGGTT ATACCCTCTG TAAACAGATA 
GGTACCCTAC TCGCTGAACT ACGGGAATAT AATCTGGAGC AGCAGCGGCG AGCCCAATGC 
GAGACCCTCT CTCCTGATGG CCTACCTGAG GAGCAACCAC AGACCACCAA ACTGAAAGGC 
AAAATGCAGA GCAGGTTTGA CATGGCTGAA AATGTGGTAA TTGTGGCATC TCAGAAGCGA 
CCCTTGGGTG GCCGGGAGCT CCAGCTGCCT TCTATGTCCA TGTTGACATC CAAGACATCT 
ACCCAGAAGT TCTTCTTGAG GGTACTACAG GTCATCATCC AGCTGCGGGA TGACACACGA 
CGAGCTAACA AGAAAGCCAA GCAGACAGGC AGGCTAGGTT CCTCCGGTTT AGGCTCAGCT 
AGCAGCATCC AGGCAGCTGT TCGGCAGCTG GAGGCTGAGG CTGATGCCAT TATACAAATG 
GTACGTGAGG GTCAAAGGGC GCGGAGACAG CAACAAGCAG CAACGTCGGA GTCTTCTAAC 
CAGTCAGAGA CATCTGTCCG GAGAGAGGAA TCGCCCATGG ATGTGGACCA GCCATCTCCT 
AGTGCTCAAG ATACTCAGTC CATTGTAATC TCAGATGGAA CACCACAGGG AGAGAAGGAG 
AAAGAGGAGA AACCACCTGA GTTACCCCTG CTCAGTGAGC AACTGAGCCT AGATGAACTG 
TGGGACATGC TAGGGGAATG TTTAAAAGAA CTGGAGGAAT CTCATGACCA GCATGCAGTA 
TTAGTGCTAC AGCCTGCTGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC 
AAGCCTCCTG TCCGAGATAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA TGAACCTCCT 
CCACTCTCCC CCGCCCCCTT AACCCCAGCC ACTCCATCCT CCCTTGACCC ATTCTTCTCC 
AGGGAGCCCT CATCTATGCA CATTTCCTCG AGCCTGCCCC CTGACACACA GAAGTTCCTT 
CGCTTTGCAG AGACTCACCG CACTGTGTTA AACCAGATAC TACGGCAGTC TACCACCCAT 
CTTGCTGATG GGCCTTTTGC TGTCTTGGTA GATTATATCC GTGTCCTCGA CTTTGATGTC 
AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG CGTTTGGACG AAGGGCTCCG GAAAGAAGAT 
ATGGCAGTGC ATGTCCGCCG TGACCATGTA TTTGAAGACT CCTATCGTGA GCTGCATCGC 
AAATCTCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG 
GATGCTGGGG GGCTCCTGCG AGAGTGGTAC ATGATCATCT CTCGAGAGAT GTTCAACCCT 
ATGTATGCCT TGTTCCGTAC CTCACCTGGT GACCGGGTCA CCTACACCAT CAATCCATCT 
TCCCACTGTA ACCCCAACCA CCTCAGCTAC TTCAAGTTTG TTGGACGAAT TGTAGCCAAA 
GCTGTATATG ACAACCGCCT CCTGGAGTGC TACTTTACTA GGTCTTTCTA CAAACACATC 
TTGGGCAAAT CTGTCAGGTA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC 
CTAGTTTATC TGCTTGAAAA TGATGTCTCT ACATTAGGCT ATGACCTCAC CTTCAGCACT 
GAGGTTCAAG AATTTGGAGT ATGTGAAGTT CGTGACCTCA AACCCAATGG AGCCAACATC 
TTGGTAACAG AGGAGAACAA GAAGGAATAT GTACACCTGG TTTGCCAGAT GAGAATGACA 
GGAGCCATCC GCAAACAGCT GGCAGCCTTC TTGGAGGGCT TCTATGAAAT CATTCCAAAG 
CGCCTCATAT CCATCTTTAC TGAGCAGGAA TTAGAGCTGC TCATATCAGG GCTGCCTACC 
ATCGACATTG ATGATCTAAA ATCTAACACT GAGTACCACA AGTACCAATC CAACTCTATT 
CAGATCCAGT GGTTTTGGAG AGCATTGCGT TCCTTTGACC AAGCAGACCG TGCCAAGTTC 
CTCCAGTTTG TCACAGGTAC TTCCAAGGTA CCCCTACAAG GATTTGCTGC CCTTGAAGGT 
ATGAATGGCA TTCAGAAGTT TCAGATTCAT CGAGATGACA GATCCACAGA TCGCCTGCCT 
TCAGCTCACA CATGTTTTAA TCAACTGGAC CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC 
CGCCACATGC TACTATTGGC CATCCAGGAA TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107316.1
CDS	541..13677
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251456.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251456.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGAGAAC AGGATGGAAC TGCTGGAAGC 
ATGGATGCAT CTGCCCAGGG TTTATTAGAA GGAATTGAAC TAGATGGTGA CACACTGGCA 
CCCATGGAGA CAGATGAACC TTCTTCTTCA GATTCTAAGG GCAAATCTAA AATCACACCT 
GCCATGGCTG CAAGAATTAA ACAAATCAAG CCGTTGCTGT CAGCTTCTTC CAGATTAGGC 
CGAGCACTTG CTGAGCTCTT TGGACTCCTT GTTAAGCTTT GTGTGGGTTC TCCTGTCCGC 
CAAAGGAGGA GCCATCATGC TGCCAGTACC ACCACTGCAC CAACACCTGC TGCTAGATCA 
ACAGCTTCAG CCCTCACTAA GCTACTCACA AAAGGCTTAT CATGGCAGCC CCCACCATAT 
ACACCCACTC CCCGCTTCAG GCTGACGTTC TTCATCTGTT CAGTTGGATT CACCTCTCCA 
ATGCTCTTTG ATGAGAGAAA GTATCCTTAC CACCTTATGC TGCAGAAATT TCTCTGCTCT 
GGTGGTCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TATCCATGGG AGGAAAAGTT 
CCTGTTTCTG AGGGATTGGA ACATTCAGAT CTACCTGATG GTACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTTGTAGA AAAGATGGTA AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGTTGCCTG CTAAATTGCC TGGAGGAGTC CAGAGCTTTC CACAATTTAG TGCCCTTCGC 
TTCCTTGTAG TAACTCAAAA AGCAGCCTTT ACTTGTATCA AGAACCTGTG GAACCGGAAA 
CCCCTTAAAG TATATGGGGG AAGAATGGCT GAGTCTATGC TGGCCATTTT GTGCCACATT 
CTCCGAGGGG AACCTGTGAT TCGAGAAAGA CTGAGCAAAG AGAAGGAAGG ATCTCGAGGA 
GAAGAAGAAG CAGGCCAGGA AGAAGGTGGC TCACGCAGAG AACCTCAAGT CAACCAGCAA 
CAGCTACAAC AGCTCATGGA CATGGGTTTC ACAAGAGAAC ATGCCATGGA GGCACTGTTG 
AATACCAGTA CGATGGAACA AGCAACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATA 
GGAGGAGTTG TTCGGGATCT TAGCATGTCA GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAAGA GCGTAAAGCT CGAGAAAAAC AAGAAGAGGA AGAAGCTAAA 
TGTCTAGAGA AGTTTCAAGA TGCTGACCCC TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACAATGT TACCAGGCTG CTTCCATCTT CTTGATGAGT TGCCTGATAC AGTGTACCGT 
GTTTGTGATC TGATCATGAC AGCTATCAAA CGTAATGGAG CTGACTATCG TGATATGATT 
CTAAAGCAAG TTGTCAACCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCTCTAACAA CAAGTGACAC AAAAACAGTT TCAGAGTGGA TCAGTCAGAT GGCTACCCTG 
CCACAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CACTACTTTT TGAGGAGTTG 
AAGTTACCTT GTGCCTGGGT GGTTGAATCA AGTGGCATTC TTAATGTCCT AATCAAACTC 
TTAGAAGTGG TTCAACCCTG CCTACAGGCT GCCAAAGAGC AAAAGGAAGT CCAGACCCCA 
AAGTGGATCA CCCCAGTGTT GCTCCTTATT GATTTTTATG AAAAGACAGC CATCTCATCA 
AAGAGAAGAG CCCAAATGAC TAAGTACCTA CAGTCCAACA GCAACAACTG GCGCTGGTTT 
GATGACCGCT CTGGGCGTTG GTGTAGTTAC AGTGCTAGCA ACAACAGCAC CATTGATTCT 
GCCTGGAAAT CTGGAGAAAC AAGTGTAAGA TTCACTGCTG GCCGACGAAG ATACACTGTC 
CAGTTCACTA CAATGGTGCA GGTTAATGAA GAAACAGGAA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTGCCACG ACTGAGTAAG AACTCAAAAA GCAGCAATGG ACAGGAACTA 
GAGAAGACAC TGGAAGAAAG CAAGGAGACC GATATCAAAC ATAAAGAAAA TAAAGGCAAT 
GATATCCCCT TAGCTCTAGA GAGCACAAAC ACTGAAAAAG AGGCAAGCCT GGATGAAACA 
AAAATTGGGG AGATCCTAAT CCAGGGCCTG ACAGAAGATA TGGTGACTGT TCTAATCCGT 
GCCTGTGTGA GCATGCTGGG AGTCCCTGTG GACCCAGACA CTTTGCATGC CACCCTTCGT 
CTCTGTTTAA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACACGAATGA TCTTGAATTT GACCCAGAGC TCAGGTTTCA ATGGGTTTAC CCCCTTAGTC 
ACCCTTCTCT TAAGGCACAT CATTGAGGAT CCTTGTACTC TTCGTCATAC TATGGAAAAG 
GTTGTTCGCT CGGCAGCCAC AAGTGGAGCT GGTAGTACTA CATCTGGTGT TGTATCTGGC 
AGCCTCGGTT CTCGGGAGAT CAACTATATC CTTAGAGTCC TAGGACCAGC TGCCTGCCGC 
AATCCAGACA TATTCACAGA AGTAGCCAAT TGCTGTATCC GCATTGCCCT TCCAGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGTCCTAAT 
GCTGTACAGC TGGTAAAGAC AACACCTTTG AAGCCCTCAT CTCTGCCTGT CATTCCTGAT 
ACTATTAAGG AAGTTATCTA TGATATGCTG AATGCTTTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGACA AATCTGATCC TAAACCTGGG GGAACGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGACGATGT ATACCAGCAG TACCGGTCAC TTACTCGTCA GAGCAGTGAC 
TTTGATACAC AGTCAGGTTT TTCCTTAAAT AGTCAGGTTT TTGCTGCAGA TGGTGCCCCT 
GCTGAAACTT CCACAACTGG AACCTCCCAA GGAGAGGGAG CTTCAACTCC AGAGGAGACT 
CGAGAAGGGA AGAAAGATAA AGAAGGGGAC CGTACATCTG AGGAAGGCAA GCAGAAAAGC 
AAGGGCAGCA AACCTTTAAT GCCTACCTCT ACTATCCTTC GTCTTCTGGC AGAGTTGGTG 
AGGTCCTATG TTGGTATTGC TACCCTGATT GCAAACTACA GCTATACCGT GGGCCAGTCT 
GAGCTGATCA AAGAGGACTG CAGTGTGCTA GCTTTTGTCC TGGACCACCT CCTCCCACAC 
ACCCAGAATG CAGAAGACAA GGATACACCT GCCCTGGCAC GTCTATTCCT TGCAAGTCTG 
GCTGCTGCGG GGAGTGGCAC AGATGCTCAA GTGGCCCTAG TGAATGAAGT GAAAGCAGCC 
CTGGGACGGG CGCTTGCTAT GGCTGAGAGT ACAGAGAAGC ACGCCAGACT TCAGGCAGTG 
ATGTGTATCA TCAGTACTAT CATGGAATCC TGCCCCTCCA CCTCCAGCTT CTACAGTAGT 
GCTACAGCCA AGACTCAGCA TAATGGCATG AACAACATCA TTCGACTGTT CCTAAAGAAG 
GGACTAGTCA ATGACCTGGC CAGAGTTCCT CACAGCCTAG ACCTGTCCAG TCCCAATATG 
GCCAACACAG TCAATGCTGC TCTAAAACCC TTGGAAACAC TTTCCCGGAT TGTAAATCAA 
CCCAGTAGCC TTTTTGGCAG CAAGAGTGCT TCTAGCAAGA ACAAGTCTGA GCAGGATGCT 
CAAGGAGCTT CTCAGGACTC CAGCAGCCAT CAGCAAGATC CAGGCGAGCC TGGGGAGGCA 
GAAGTGCAGG AGGAGGATCA TGATGTCACT CAGACAGAGG TGGCAGATGG GGATATCATG 
GATGGGGAGG CTGAAACCGA CTCAGTGGTG ATTGCCGGGC AGCCTGAGGT GCTCAGTTCA 
CAAGAGATGC AGGTTGAAAA TGAACTGGAG GACCTGATAG ATGAGTTGCT TGAGAGGGAT 
GGCGGATCTG GGAACAGTAC AATTATAGTG AGCAGAAGTG GAGAGGATGA ATCACAAGAG 
GACGTGCTGA TGGATGAAGC TCCTTCCAAC CTCAGCCAAG CTTCCACCTT GCAGGCCAAC 
CGAGAAGATT CCATGAATAT CCTGGACCCT GAGGATGAGG AGGAACATAC TCAGGAAGAG 
GACAGCAGTG GCAGTAACGA GGATGAGGAT GATAGTCAGG ATGAAGAGGA GGAGGAGGAG 
GAAGATGAGG AAGATGATCA GGAGGATGAT GAAGGTGAAG AAGGAGATGA AGATGATGAC 
GACGATGGTT CTGAGATGGA ATTGGATGAG GATTACCCTG ATATGAACGC TTCTCCCTTG 
GTCCGATTTG AGCGCTTTGA CCGGGAGGAT GATCTCATCA TTGAGTTTGA CAACATGTTC 
TCCAGTGCTA CAGACATTCC CCCATCCCCA GGAAATATCC CTACCACCCA CCCATTGATG 
GTACGTCATG CAGATCACAG TTCCCTGACA CTGGGCAGTG GTTCTTCCAC AACTCGTCTT 
ACCCAGGGCA TCGGTCGCAG TCAAAGGACC CTCAGACAGC TCACTGCCAA TACAGGCCAT 
ACCATTCATG TTCACTACCC TGGGAATCGC CAGCCTAACC CTCCTCTTAT ACTCCAGAGG 
TTGCTTGGTC CCTCTGCAGC TGCTGACATT CTTCAGCTGA GCAGCAGCCT TCCATTACAA 
AGCCGAGGCC GGGCCCGCCT CCTAGTGGGC AATGATGACG TCCATATCAT CGCCAGGTCT 
GATGACGAGC TGCTGGATGA CTTTTTTCAT GACCAGAGTA CAGCCACTAG CCAAGCAGGA 
ACCCTGTCCA GCATCCCAAC AGCTCTAACC CGATGGACAG AGGAATGCAA AGTTCTTGAT 
GCTGAGAGTA TGCATGACTG TGTTTCAGTG GTTAAAGTGC CCATTGTCAA TCACCTGGAA 
TTCTTGAGAG ATGAGGAGCT AGAAGAGAGA AGGGAGAAAC GCAGGAAACA ATTGGCTGAG 
GAAGAAACAA AAATAATAGA CAAAGGCAAA GAAGACAAGG AGAACAGAGA TCAGAGTGCA 
CAGTGTACTG TAACTAAGAC AAATGACTCC ACTGAACAGA ATGTCTCAGA TGGGACTCCT 
ATGCCTGACA GCTATCCAAC AACCCCCTCT TCAACGGATG CACCTACATC TGAGTCCAAG 
GAAACACTTG GTACCCTACA ACCCTCCCAG CAACAGCCAG CACTTCCACC CCCACCATCC 
TTAGGAGAGA TTCCTCAGGA ACTACAGTCC CCTGCTGAAG AAGTGGCAAA CTCTACACAG 
CTATTGATGC CTATAGAGTT GGAGGAATTG GGTCCCACAA GACCAAGTGG AGAAGCAGAA 
ACAACTCAAA TGGAATTGTC TCCAGCTCCC ACCATAACCT CTCTTTCCCC AGAGAGAGCT 
GAAGATTCAG ACGCGTTAAC AGCTGTCAGC AGTCAGCTGG AAGGTTCTCC CATGGACACC 
AGCAGCCTGG CTTCCTGTAC GTTAGAAGAG GCTGTGGGTG ACACTCCAGC AGCTGGCAGT 
TCTGAGCAGC CCACAGCAGG TAGTTCCACT CCTGGGGATG CGCCATCAGT TGTGGCAGAA 
GTGCAAGGCA GACCTGATGT ATCAAGAGAA TCCAACCAGC CACCTGAGGA CAGCTCCCCC 
CCTGCATCCT CGGAGAGTTC TTCCACTAGA GATTCTGCCG TGGCCATCTC TGGAGCAGAT 
TCTCGTGGAA TTCTAGAAGA GCCACTACCT TCAACAAGCA GTGAAGAAGA AGATCCCCTT 
GCTGGTATCA GTCTCCCTGA AGGTGTGGAT CCCTCCTTTC TGGCTGCTCT CCCTGATGAT 
ATCCGTCGGG AAGTCCTACA GAACCAGCTG GGCATACGTC CACCAACCCG AAGTGCTCCC 
TCCTCAAATA GCTCAGCTCC TGCAGTAGTG GGGAACCCTG GTGTAACTGA AGTGAGCCCT 
GAGTTCCTGG CTGCCCTACC TCCAGCTATT CAAGAGGAAG TGCTAGCACA GCAGAGAGCT 
GAACAGCAGC GACGGGAACT GGCTCAGAAT GCGAGCTCAG ACACCCCCAT GGACCCTGTG 
ACTTTCATTC AGACTTTGCC CTCAGATCTG CGCCGTAGTG TCCTAGAGGA TATGGAAGAC 
AGTGTGTTAG CTGTGATGCC ACCTGACATT GCAGCTGAGG CTCAAGCTCT AAGACGAGAA 
CAAGAAGCCC GACAGCGGCA GCTCATGCAT GAGCGTCTTT TTGGACACAG TAGTACTTCT 
GCACTCTCTG CCATTCTCCG AAGCCCGGCT TTTACCAGTC GCCTGAGTGG CAACCGTGGG 
GTCCAGTATA CTCGCCTTGC TGTACAGAGG GGTGGCACCT TCCAGATGGG GGGTAGCAGC 
AGCCATAATA GGCCTTCTGG CAGTAATGTG GACACACTAC TCCGCCTCCG AGGACGACTC 
CTTCTAGACC ATGAAGCCCT GTCTTGCCTC TTGGTCCTAC TTTTTGTGGA TGAGCCAAAG 
CTCAATACTA GCCGTCTACA CCGAGTACTG AGAAATCTCT GCTATCATGC CCAGACCCGC 
CACTGGGTCA TCCGCAGCCT CCTGTCCATT TTGCAGCGCA GCAGTGAAAG TGAGTTATGC 
ATTGAGACAC CTAAGCTCTC CACAAGTGAA GAAAGGGGCA AGAAATCAAG CAAGAGCTGT 
GCATCAAGCA GTCATGAGAA CCGTCCCCTG GATCTGCTCC ACAAGATGGA GTCCAAAAGT 
TCCAATCAGC TCTCCTGGCT CTCAGTATCA ATGGATGCTG CTTTAGGCTG TAGGACTAAC 
ATATTCCAGA TTCAACGTTC TGGAGGGCGT AAACATACAG AGAAGCATGC AAGCAGTGGT 
TCCACTGTCC ACATCCACCC CCAGGCTGCT CCTGTTGTTT GCAGACATGT TTTGGATACA 
CTCATTCAGT TGGCCAAGGT GTTTCCCAGC CACTTTACAC AGCAGCGGAC CAAAGAAACA 
AACTGTGAGA GTGATCGGGA ACGGGGCAGT AAGCAGGCCT GCAGCCCATG CTCCTCACAG 
TCCTCCAGCA GTGGCATCTG TACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG 
AATGTTAGCC GAAAAGGCAA GAACTCCGTG AAGTCAGTAC CAGTGAGCTC TGGTGGTGAA 
GGGGAAACTT CCCCACACAG CCTTGAAGCC TCTCCACTGG GGCAGCTCAT GAACATGTTG 
TCACATCCAG TCATCCGCCG GAGTTCTCTC TTAACTGAGA AACTCCTGAG ACTCCTCTCT 
CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTATCAGAAG TGCAAACTAA CTCCAGCAAC 
AGTGGTTCCT CCACTGCTGC CACCTCAAAC ACATCTACTA CTACCACCAC CACCACTACT 
GCCACTGCCC CCACGCCCAC ACCCCCAGCT GCAACTACCC CTGTCACTTC TGCTCCAGCT 
CTGGTTGCTG CCACAGCTAT TTCCACCATT ACTGTAGCTG CTTCTACCAC AGTGACTACC 
CCCACAACTG CTACCACTAC TGTTTCAACT TCTACTACTA AAGGCAGCAA ATCTCCAGCA 
AAGGTTGGTG AAGGAGGCAG TGGTATAGAC TTCAAAATGG TGTCCTCTGG CCTCACTGAA 
AACCAGCTAC AGCTCTCTGT AGAGGTGTTG ACATCCCACT CTTGTTCTGA AGAAGGCTTG 
GAAGATGCGG CCAATGTGTT ACTGCAGCTC TCTCGTGGGG ACTCTGGTAC CCGGGACACT 
GTTCTTAAGC TGCTACTAAA TGGAGCCCGC CATCTGGGTT ATACCCTCTG TAAACAGATA 
GGTACCCTAC TCGCTGAACT ACGGGAATAT AATCTGGAGC AGCAGCGGCG AGCCCAATGC 
GAGACCCTCT CTCCTGATGG CCTACCTGAG GAGCAACCAC AGACCACCAA ACTGAAAGGC 
AAAATGCAGA GCAGGTTTGA CATGGCTGAA AATGTGGTAA TTGTGGCATC TCAGAAGCGA 
CCCTTGGGTG GCCGGGAGCT CCAGCTGCCT TCTATGTCCA TGTTGACATC CAAGACATCT 
ACCCAGAAGT TCTTCTTGAG GGTACTACAG GTCATCATCC AGCTGCGGGA TGACACACGA 
CGAGCTAACA AGAAAGCCAA GCAGACAGGC AGGCTAGGTT CCTCCGGTTT AGGCTCAGCT 
AGCAGCATCC AGGCAGCTGT TCGGCAGCTG GAGGCTGAGG CTGATGCCAT TATACAAATG 
GTACGTGAGG GTCAAAGGGC GCGGAGACAG CAACAAGCAG CAACGTCGGA GTCTTCTAAC 
CAGTCAGAGA CATCTGTCCG GAGAGAGGAA TCGCCCATGG ATGTGGACCA GCCATCTCCT 
AGTGCTCAAG ATACTCAGTC CATTGTAATC TCAGATGGAA CACCACAGGG AGAGAAGGAG 
AAAGAGGAGA AACCACCTGA GTTACCCCTG CTCAGTGAGC AACTGAGCCT AGATGAACTG 
TGGGACATGC TAGGGGAATG TTTAAAAGAA CTGGAGGAAT CTCATGACCA GCATGCAGTA 
TTAGTGCTAC AGCCTGCTGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC 
AAGCCTCCTG TCCGAGATAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA TGAACCTCCT 
CCACTCTCCC CCGCCCCCTT AACCCCAGCC ACTCCATCCT CCCTTGACCC ATTCTTCTCC 
AGGGAGCCCT CATCTATGCA CATTTCCTCG AGCCTGCCCC CTGACACACA GAAGTTCCTT 
CGCTTTGCAG AGACTCACCG CACTGTGTTA AACCAGATAC TACGGCAGTC TACCACCCAT 
CTTGCTGATG GGCCTTTTGC TGTCTTGGTA GATTATATCC GTGTCCTCGA CTTTGATGTC 
AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG CGTTTGGACG AAGGGCTCCG GAAAGAAGAT 
ATGGCAGTGC ATGTCCGCCG TGACCATGTA TTTGAAGACT CCTATCGTGA GCTGCATCGC 
AAATCTCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG 
GATGCTGGGG GGCTCCTGCG AGAGTGGTAC ATGATCATCT CTCGAGAGAT GTTCAACCCT 
ATGTATGCCT TGTTCCGTAC CTCACCTGGT GACCGGGTCA CCTACACCAT CAATCCATCT 
TCCCACTGTA ACCCCAACCA CCTCAGCTAC TTCAAGTTTG TTGGACGAAT TGTAGCCAAA 
GCTGTATATG ACAACCGCCT CCTGGAGTGC TACTTTACTA GGTCTTTCTA CAAACACATC 
TTGGGCAAAT CTGTCAGGTA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC 
CTAGTTTATC TGCTTGAAAA TGATGTCTCT ACATTAGGCT ATGACCTCAC CTTCAGCACT 
GAGGTTCAAG AATTTGGAGT ATGTGAAGTT CGTGACCTCA AACCCAATGG AGCCAACATC 
TTGGTAACAG AGGAGAACAA GAAGGAATAT GTACACCTGG TTTGCCAGAT GAGAATGACA 
GGAGCCATCC GCAAACAGCT GGCAGCCTTC TTGGAGGGCT TCTATGAAAT CATTCCAAAG 
CGCCTCATAT CCATCTTTAC TGAGCAGGAA TTAGAGCTGC TCATATCAGG GCTGCCTACC 
ATCGACATTG ATGATCTAAA ATCTAACACT GAGTACCACA AGTACCAATC CAACTCTATT 
CAGATCCAGT GGTTTTGGAG AGCATTGCGT TCCTTTGACC AAGCAGACCG TGCCAAGTTC 
CTCCAGTTTG TCACAGGTAC TTCCAAGGTA CCCCTACAAG GATTTGCTGC CCTTGAAGGT 
ATGAATGGCA TTCAGAAGTT TCAGATTCAT CGAGATGACA GATCCACAGA TCGCCTGCCT 
TCAGCTCACA CATGTTTTAA TCAACTGGAC CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC 
CGCCACATGC TACTATTGGC CATCCAGGAA TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528949.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529012.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X19, mRNA.

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CloneID	OMu15194
Clone ID Related Accession (Same CDS sequence)	XM_011247858.1 , XM_006528949.4 , XM_030251455.1 , XM_030251456.1 , XM_006528949.3 , NM_021523.4
Accession Version	XM_006528949.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528949.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGAGAAC AGGATGGAAC TGCTGGAAGC 
ATGGATGCAT CTGCCCAGGG TTTATTAGAA GGAATTGAAC TAGATGGTGA CACACTGGCA 
CCCATGGAGA CAGATGAACC TTCTTCTTCA GATTCTAAGG GCAAATCTAA AATCACACCT 
GCCATGGCTG CAAGAATTAA ACAAATCAAG CCGTTGCTGT CAGCTTCTTC CAGATTAGGC 
CGAGCACTTG CTGAGCTCTT TGGACTCCTT GTTAAGCTTT GTGTGGGTTC TCCTGTCCGC 
CAAAGGAGGA GCCATCATGC TGCCAGTACC ACCACTGCAC CAACACCTGC TGCTAGATCA 
ACAGCTTCAG CCCTCACTAA GCTACTCACA AAAGGCTTAT CATGGCAGCC CCCACCATAT 
ACACCCACTC CCCGCTTCAG GCTGACGTTC TTCATCTGTT CAGTTGGATT CACCTCTCCA 
ATGCTCTTTG ATGAGAGAAA GTATCCTTAC CACCTTATGC TGCAGAAATT TCTCTGCTCT 
GGTGGTCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TATCCATGGG AGGAAAAGTT 
CCTGTTTCTG AGGGATTGGA ACATTCAGAT CTACCTGATG GTACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTTGTAGA AAAGATGGTA AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGTTGCCTG CTAAATTGCC TGGAGGAGTC CAGAGCTTTC CACAATTTAG TGCCCTTCGC 
TTCCTTGTAG TAACTCAAAA AGCAGCCTTT ACTTGTATCA AGAACCTGTG GAACCGGAAA 
CCCCTTAAAG TATATGGGGG AAGAATGGCT GAGTCTATGC TGGCCATTTT GTGCCACATT 
CTCCGAGGGG AACCTGTGAT TCGAGAAAGA CTGAGCAAAG AGAAGGAAGG ATCTCGAGGA 
GAAGAAGAAG CAGGCCAGGA AGAAGGTGGC TCACGCAGAG AACCTCAAGT CAACCAGCAA 
CAGCTACAAC AGCTCATGGA CATGGGTTTC ACAAGAGAAC ATGCCATGGA GGCACTGTTG 
AATACCAGTA CGATGGAACA AGCAACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATA 
GGAGGAGTTG TTCGGGATCT TAGCATGTCA GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAAGA GCGTAAAGCT CGAGAAAAAC AAGAAGAGGA AGAAGCTAAA 
TGTCTAGAGA AGTTTCAAGA TGCTGACCCC TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACAATGT TACCAGGCTG CTTCCATCTT CTTGATGAGT TGCCTGATAC AGTGTACCGT 
GTTTGTGATC TGATCATGAC AGCTATCAAA CGTAATGGAG CTGACTATCG TGATATGATT 
CTAAAGCAAG TTGTCAACCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCTCTAACAA CAAGTGACAC AAAAACAGTT TCAGAGTGGA TCAGTCAGAT GGCTACCCTG 
CCACAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CACTACTTTT TGAGGAGTTG 
AAGTTACCTT GTGCCTGGGT GGTTGAATCA AGTGGCATTC TTAATGTCCT AATCAAACTC 
TTAGAAGTGG TTCAACCCTG CCTACAGGCT GCCAAAGAGC AAAAGGAAGT CCAGACCCCA 
AAGTGGATCA CCCCAGTGTT GCTCCTTATT GATTTTTATG AAAAGACAGC CATCTCATCA 
AAGAGAAGAG CCCAAATGAC TAAGTACCTA CAGTCCAACA GCAACAACTG GCGCTGGTTT 
GATGACCGCT CTGGGCGTTG GTGTAGTTAC AGTGCTAGCA ACAACAGCAC CATTGATTCT 
GCCTGGAAAT CTGGAGAAAC AAGTGTAAGA TTCACTGCTG GCCGACGAAG ATACACTGTC 
CAGTTCACTA CAATGGTGCA GGTTAATGAA GAAACAGGAA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTGCCACG ACTGAGTAAG AACTCAAAAA GCAGCAATGG ACAGGAACTA 
GAGAAGACAC TGGAAGAAAG CAAGGAGACC GATATCAAAC ATAAAGAAAA TAAAGGCAAT 
GATATCCCCT TAGCTCTAGA GAGCACAAAC ACTGAAAAAG AGGCAAGCCT GGATGAAACA 
AAAATTGGGG AGATCCTAAT CCAGGGCCTG ACAGAAGATA TGGTGACTGT TCTAATCCGT 
GCCTGTGTGA GCATGCTGGG AGTCCCTGTG GACCCAGACA CTTTGCATGC CACCCTTCGT 
CTCTGTTTAA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACACGAATGA TCTTGAATTT GACCCAGAGC TCAGGTTTCA ATGGGTTTAC CCCCTTAGTC 
ACCCTTCTCT TAAGGCACAT CATTGAGGAT CCTTGTACTC TTCGTCATAC TATGGAAAAG 
GTTGTTCGCT CGGCAGCCAC AAGTGGAGCT GGTAGTACTA CATCTGGTGT TGTATCTGGC 
AGCCTCGGTT CTCGGGAGAT CAACTATATC CTTAGAGTCC TAGGACCAGC TGCCTGCCGC 
AATCCAGACA TATTCACAGA AGTAGCCAAT TGCTGTATCC GCATTGCCCT TCCAGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGTCCTAAT 
GCTGTACAGC TGGTAAAGAC AACACCTTTG AAGCCCTCAT CTCTGCCTGT CATTCCTGAT 
ACTATTAAGG AAGTTATCTA TGATATGCTG AATGCTTTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGACA AATCTGATCC TAAACCTGGG GGAACGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGACGATGT ATACCAGCAG TACCGGTCAC TTACTCGTCA GAGCAGTGAC 
TTTGATACAC AGTCAGGTTT TTCCTTAAAT AGTCAGGTTT TTGCTGCAGA TGGTGCCCCT 
GCTGAAACTT CCACAACTGG AACCTCCCAA GGAGAGGGAG CTTCAACTCC AGAGGAGACT 
CGAGAAGGGA AGAAAGATAA AGAAGGGGAC CGTACATCTG AGGAAGGCAA GCAGAAAAGC 
AAGGGCAGCA AACCTTTAAT GCCTACCTCT ACTATCCTTC GTCTTCTGGC AGAGTTGGTG 
AGGTCCTATG TTGGTATTGC TACCCTGATT GCAAACTACA GCTATACCGT GGGCCAGTCT 
GAGCTGATCA AAGAGGACTG CAGTGTGCTA GCTTTTGTCC TGGACCACCT CCTCCCACAC 
ACCCAGAATG CAGAAGACAA GGATACACCT GCCCTGGCAC GTCTATTCCT TGCAAGTCTG 
GCTGCTGCGG GGAGTGGCAC AGATGCTCAA GTGGCCCTAG TGAATGAAGT GAAAGCAGCC 
CTGGGACGGG CGCTTGCTAT GGCTGAGAGT ACAGAGAAGC ACGCCAGACT TCAGGCAGTG 
ATGTGTATCA TCAGTACTAT CATGGAATCC TGCCCCTCCA CCTCCAGCTT CTACAGTAGT 
GCTACAGCCA AGACTCAGCA TAATGGCATG AACAACATCA TTCGACTGTT CCTAAAGAAG 
GGACTAGTCA ATGACCTGGC CAGAGTTCCT CACAGCCTAG ACCTGTCCAG TCCCAATATG 
GCCAACACAG TCAATGCTGC TCTAAAACCC TTGGAAACAC TTTCCCGGAT TGTAAATCAA 
CCCAGTAGCC TTTTTGGCAG CAAGAGTGCT TCTAGCAAGA ACAAGTCTGA GCAGGATGCT 
CAAGGAGCTT CTCAGGACTC CAGCAGCCAT CAGCAAGATC CAGGCGAGCC TGGGGAGGCA 
GAAGTGCAGG AGGAGGATCA TGATGTCACT CAGACAGAGG TGGCAGATGG GGATATCATG 
GATGGGGAGG CTGAAACCGA CTCAGTGGTG ATTGCCGGGC AGCCTGAGGT GCTCAGTTCA 
CAAGAGATGC AGGTTGAAAA TGAACTGGAG GACCTGATAG ATGAGTTGCT TGAGAGGGAT 
GGCGGATCTG GGAACAGTAC AATTATAGTG AGCAGAAGTG GAGAGGATGA ATCACAAGAG 
GACGTGCTGA TGGATGAAGC TCCTTCCAAC CTCAGCCAAG CTTCCACCTT GCAGGCCAAC 
CGAGAAGATT CCATGAATAT CCTGGACCCT GAGGATGAGG AGGAACATAC TCAGGAAGAG 
GACAGCAGTG GCAGTAACGA GGATGAGGAT GATAGTCAGG ATGAAGAGGA GGAGGAGGAG 
GAAGATGAGG AAGATGATCA GGAGGATGAT GAAGGTGAAG AAGGAGATGA AGATGATGAC 
GACGATGGTT CTGAGATGGA ATTGGATGAG GATTACCCTG ATATGAACGC TTCTCCCTTG 
GTCCGATTTG AGCGCTTTGA CCGGGAGGAT GATCTCATCA TTGAGTTTGA CAACATGTTC 
TCCAGTGCTA CAGACATTCC CCCATCCCCA GGAAATATCC CTACCACCCA CCCATTGATG 
GTACGTCATG CAGATCACAG TTCCCTGACA CTGGGCAGTG GTTCTTCCAC AACTCGTCTT 
ACCCAGGGCA TCGGTCGCAG TCAAAGGACC CTCAGACAGC TCACTGCCAA TACAGGCCAT 
ACCATTCATG TTCACTACCC TGGGAATCGC CAGCCTAACC CTCCTCTTAT ACTCCAGAGG 
TTGCTTGGTC CCTCTGCAGC TGCTGACATT CTTCAGCTGA GCAGCAGCCT TCCATTACAA 
AGCCGAGGCC GGGCCCGCCT CCTAGTGGGC AATGATGACG TCCATATCAT CGCCAGGTCT 
GATGACGAGC TGCTGGATGA CTTTTTTCAT GACCAGAGTA CAGCCACTAG CCAAGCAGGA 
ACCCTGTCCA GCATCCCAAC AGCTCTAACC CGATGGACAG AGGAATGCAA AGTTCTTGAT 
GCTGAGAGTA TGCATGACTG TGTTTCAGTG GTTAAAGTGC CCATTGTCAA TCACCTGGAA 
TTCTTGAGAG ATGAGGAGCT AGAAGAGAGA AGGGAGAAAC GCAGGAAACA ATTGGCTGAG 
GAAGAAACAA AAATAATAGA CAAAGGCAAA GAAGACAAGG AGAACAGAGA TCAGAGTGCA 
CAGTGTACTG TAACTAAGAC AAATGACTCC ACTGAACAGA ATGTCTCAGA TGGGACTCCT 
ATGCCTGACA GCTATCCAAC AACCCCCTCT TCAACGGATG CACCTACATC TGAGTCCAAG 
GAAACACTTG GTACCCTACA ACCCTCCCAG CAACAGCCAG CACTTCCACC CCCACCATCC 
TTAGGAGAGA TTCCTCAGGA ACTACAGTCC CCTGCTGAAG AAGTGGCAAA CTCTACACAG 
CTATTGATGC CTATAGAGTT GGAGGAATTG GGTCCCACAA GACCAAGTGG AGAAGCAGAA 
ACAACTCAAA TGGAATTGTC TCCAGCTCCC ACCATAACCT CTCTTTCCCC AGAGAGAGCT 
GAAGATTCAG ACGCGTTAAC AGCTGTCAGC AGTCAGCTGG AAGGTTCTCC CATGGACACC 
AGCAGCCTGG CTTCCTGTAC GTTAGAAGAG GCTGTGGGTG ACACTCCAGC AGCTGGCAGT 
TCTGAGCAGC CCACAGCAGG TAGTTCCACT CCTGGGGATG CGCCATCAGT TGTGGCAGAA 
GTGCAAGGCA GACCTGATGT ATCAAGAGAA TCCAACCAGC CACCTGAGGA CAGCTCCCCC 
CCTGCATCCT CGGAGAGTTC TTCCACTAGA GATTCTGCCG TGGCCATCTC TGGAGCAGAT 
TCTCGTGGAA TTCTAGAAGA GCCACTACCT TCAACAAGCA GTGAAGAAGA AGATCCCCTT 
GCTGGTATCA GTCTCCCTGA AGGTGTGGAT CCCTCCTTTC TGGCTGCTCT CCCTGATGAT 
ATCCGTCGGG AAGTCCTACA GAACCAGCTG GGCATACGTC CACCAACCCG AAGTGCTCCC 
TCCTCAAATA GCTCAGCTCC TGCAGTAGTG GGGAACCCTG GTGTAACTGA AGTGAGCCCT 
GAGTTCCTGG CTGCCCTACC TCCAGCTATT CAAGAGGAAG TGCTAGCACA GCAGAGAGCT 
GAACAGCAGC GACGGGAACT GGCTCAGAAT GCGAGCTCAG ACACCCCCAT GGACCCTGTG 
ACTTTCATTC AGACTTTGCC CTCAGATCTG CGCCGTAGTG TCCTAGAGGA TATGGAAGAC 
AGTGTGTTAG CTGTGATGCC ACCTGACATT GCAGCTGAGG CTCAAGCTCT AAGACGAGAA 
CAAGAAGCCC GACAGCGGCA GCTCATGCAT GAGCGTCTTT TTGGACACAG TAGTACTTCT 
GCACTCTCTG CCATTCTCCG AAGCCCGGCT TTTACCAGTC GCCTGAGTGG CAACCGTGGG 
GTCCAGTATA CTCGCCTTGC TGTACAGAGG GGTGGCACCT TCCAGATGGG GGGTAGCAGC 
AGCCATAATA GGCCTTCTGG CAGTAATGTG GACACACTAC TCCGCCTCCG AGGACGACTC 
CTTCTAGACC ATGAAGCCCT GTCTTGCCTC TTGGTCCTAC TTTTTGTGGA TGAGCCAAAG 
CTCAATACTA GCCGTCTACA CCGAGTACTG AGAAATCTCT GCTATCATGC CCAGACCCGC 
CACTGGGTCA TCCGCAGCCT CCTGTCCATT TTGCAGCGCA GCAGTGAAAG TGAGTTATGC 
ATTGAGACAC CTAAGCTCTC CACAAGTGAA GAAAGGGGCA AGAAATCAAG CAAGAGCTGT 
GCATCAAGCA GTCATGAGAA CCGTCCCCTG GATCTGCTCC ACAAGATGGA GTCCAAAAGT 
TCCAATCAGC TCTCCTGGCT CTCAGTATCA ATGGATGCTG CTTTAGGCTG TAGGACTAAC 
ATATTCCAGA TTCAACGTTC TGGAGGGCGT AAACATACAG AGAAGCATGC AAGCAGTGGT 
TCCACTGTCC ACATCCACCC CCAGGCTGCT CCTGTTGTTT GCAGACATGT TTTGGATACA 
CTCATTCAGT TGGCCAAGGT GTTTCCCAGC CACTTTACAC AGCAGCGGAC CAAAGAAACA 
AACTGTGAGA GTGATCGGGA ACGGGGCAGT AAGCAGGCCT GCAGCCCATG CTCCTCACAG 
TCCTCCAGCA GTGGCATCTG TACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG 
AATGTTAGCC GAAAAGGCAA GAACTCCGTG AAGTCAGTAC CAGTGAGCTC TGGTGGTGAA 
GGGGAAACTT CCCCACACAG CCTTGAAGCC TCTCCACTGG GGCAGCTCAT GAACATGTTG 
TCACATCCAG TCATCCGCCG GAGTTCTCTC TTAACTGAGA AACTCCTGAG ACTCCTCTCT 
CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTATCAGAAG TGCAAACTAA CTCCAGCAAC 
AGTGGTTCCT CCACTGCTGC CACCTCAAAC ACATCTACTA CTACCACCAC CACCACTACT 
GCCACTGCCC CCACGCCCAC ACCCCCAGCT GCAACTACCC CTGTCACTTC TGCTCCAGCT 
CTGGTTGCTG CCACAGCTAT TTCCACCATT ACTGTAGCTG CTTCTACCAC AGTGACTACC 
CCCACAACTG CTACCACTAC TGTTTCAACT TCTACTACTA AAGGCAGCAA ATCTCCAGCA 
AAGGTTGGTG AAGGAGGCAG TGGTATAGAC TTCAAAATGG TGTCCTCTGG CCTCACTGAA 
AACCAGCTAC AGCTCTCTGT AGAGGTGTTG ACATCCCACT CTTGTTCTGA AGAAGGCTTG 
GAAGATGCGG CCAATGTGTT ACTGCAGCTC TCTCGTGGGG ACTCTGGTAC CCGGGACACT 
GTTCTTAAGC TGCTACTAAA TGGAGCCCGC CATCTGGGTT ATACCCTCTG TAAACAGATA 
GGTACCCTAC TCGCTGAACT ACGGGAATAT AATCTGGAGC AGCAGCGGCG AGCCCAATGC 
GAGACCCTCT CTCCTGATGG CCTACCTGAG GAGCAACCAC AGACCACCAA ACTGAAAGGC 
AAAATGCAGA GCAGGTTTGA CATGGCTGAA AATGTGGTAA TTGTGGCATC TCAGAAGCGA 
CCCTTGGGTG GCCGGGAGCT CCAGCTGCCT TCTATGTCCA TGTTGACATC CAAGACATCT 
ACCCAGAAGT TCTTCTTGAG GGTACTACAG GTCATCATCC AGCTGCGGGA TGACACACGA 
CGAGCTAACA AGAAAGCCAA GCAGACAGGC AGGCTAGGTT CCTCCGGTTT AGGCTCAGCT 
AGCAGCATCC AGGCAGCTGT TCGGCAGCTG GAGGCTGAGG CTGATGCCAT TATACAAATG 
GTACGTGAGG GTCAAAGGGC GCGGAGACAG CAACAAGCAG CAACGTCGGA GTCTTCTAAC 
CAGTCAGAGA CATCTGTCCG GAGAGAGGAA TCGCCCATGG ATGTGGACCA GCCATCTCCT 
AGTGCTCAAG ATACTCAGTC CATTGTAATC TCAGATGGAA CACCACAGGG AGAGAAGGAG 
AAAGAGGAGA AACCACCTGA GTTACCCCTG CTCAGTGAGC AACTGAGCCT AGATGAACTG 
TGGGACATGC TAGGGGAATG TTTAAAAGAA CTGGAGGAAT CTCATGACCA GCATGCAGTA 
TTAGTGCTAC AGCCTGCTGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC 
AAGCCTCCTG TCCGAGATAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA TGAACCTCCT 
CCACTCTCCC CCGCCCCCTT AACCCCAGCC ACTCCATCCT CCCTTGACCC ATTCTTCTCC 
AGGGAGCCCT CATCTATGCA CATTTCCTCG AGCCTGCCCC CTGACACACA GAAGTTCCTT 
CGCTTTGCAG AGACTCACCG CACTGTGTTA AACCAGATAC TACGGCAGTC TACCACCCAT 
CTTGCTGATG GGCCTTTTGC TGTCTTGGTA GATTATATCC GTGTCCTCGA CTTTGATGTC 
AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG CGTTTGGACG AAGGGCTCCG GAAAGAAGAT 
ATGGCAGTGC ATGTCCGCCG TGACCATGTA TTTGAAGACT CCTATCGTGA GCTGCATCGC 
AAATCTCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG 
GATGCTGGGG GGCTCCTGCG AGAGTGGTAC ATGATCATCT CTCGAGAGAT GTTCAACCCT 
ATGTATGCCT TGTTCCGTAC CTCACCTGGT GACCGGGTCA CCTACACCAT CAATCCATCT 
TCCCACTGTA ACCCCAACCA CCTCAGCTAC TTCAAGTTTG TTGGACGAAT TGTAGCCAAA 
GCTGTATATG ACAACCGCCT CCTGGAGTGC TACTTTACTA GGTCTTTCTA CAAACACATC 
TTGGGCAAAT CTGTCAGGTA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC 
CTAGTTTATC TGCTTGAAAA TGATGTCTCT ACATTAGGCT ATGACCTCAC CTTCAGCACT 
GAGGTTCAAG AATTTGGAGT ATGTGAAGTT CGTGACCTCA AACCCAATGG AGCCAACATC 
TTGGTAACAG AGGAGAACAA GAAGGAATAT GTACACCTGG TTTGCCAGAT GAGAATGACA 
GGAGCCATCC GCAAACAGCT GGCAGCCTTC TTGGAGGGCT TCTATGAAAT CATTCCAAAG 
CGCCTCATAT CCATCTTTAC TGAGCAGGAA TTAGAGCTGC TCATATCAGG GCTGCCTACC 
ATCGACATTG ATGATCTAAA ATCTAACACT GAGTACCACA AGTACCAATC CAACTCTATT 
CAGATCCAGT GGTTTTGGAG AGCATTGCGT TCCTTTGACC AAGCAGACCG TGCCAAGTTC 
CTCCAGTTTG TCACAGGTAC TTCCAAGGTA CCCCTACAAG GATTTGCTGC CCTTGAAGGT 
ATGAATGGCA TTCAGAAGTT TCAGATTCAT CGAGATGACA GATCCACAGA TCGCCTGCCT 
TCAGCTCACA CATGTTTTAA TCAACTGGAC CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC 
CGCCACATGC TACTATTGGC CATCCAGGAA TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529012.1
CDS	913..14049
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528949.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528949.3

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGAGAAC AGGATGGAAC TGCTGGAAGC 
ATGGATGCAT CTGCCCAGGG TTTATTAGAA GGAATTGAAC TAGATGGTGA CACACTGGCA 
CCCATGGAGA CAGATGAACC TTCTTCTTCA GATTCTAAGG GCAAATCTAA AATCACACCT 
GCCATGGCTG CAAGAATTAA ACAAATCAAG CCGTTGCTGT CAGCTTCTTC CAGATTAGGC 
CGAGCACTTG CTGAGCTCTT TGGACTCCTT GTTAAGCTTT GTGTGGGTTC TCCTGTCCGC 
CAAAGGAGGA GCCATCATGC TGCCAGTACC ACCACTGCAC CAACACCTGC TGCTAGATCA 
ACAGCTTCAG CCCTCACTAA GCTACTCACA AAAGGCTTAT CATGGCAGCC CCCACCATAT 
ACACCCACTC CCCGCTTCAG GCTGACGTTC TTCATCTGTT CAGTTGGATT CACCTCTCCA 
ATGCTCTTTG ATGAGAGAAA GTATCCTTAC CACCTTATGC TGCAGAAATT TCTCTGCTCT 
GGTGGTCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TATCCATGGG AGGAAAAGTT 
CCTGTTTCTG AGGGATTGGA ACATTCAGAT CTACCTGATG GTACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTTGTAGA AAAGATGGTA AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGTTGCCTG CTAAATTGCC TGGAGGAGTC CAGAGCTTTC CACAATTTAG TGCCCTTCGC 
TTCCTTGTAG TAACTCAAAA AGCAGCCTTT ACTTGTATCA AGAACCTGTG GAACCGGAAA 
CCCCTTAAAG TATATGGGGG AAGAATGGCT GAGTCTATGC TGGCCATTTT GTGCCACATT 
CTCCGAGGGG AACCTGTGAT TCGAGAAAGA CTGAGCAAAG AGAAGGAAGG ATCTCGAGGA 
GAAGAAGAAG CAGGCCAGGA AGAAGGTGGC TCACGCAGAG AACCTCAAGT CAACCAGCAA 
CAGCTACAAC AGCTCATGGA CATGGGTTTC ACAAGAGAAC ATGCCATGGA GGCACTGTTG 
AATACCAGTA CGATGGAACA AGCAACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATA 
GGAGGAGTTG TTCGGGATCT TAGCATGTCA GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAAGA GCGTAAAGCT CGAGAAAAAC AAGAAGAGGA AGAAGCTAAA 
TGTCTAGAGA AGTTTCAAGA TGCTGACCCC TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACAATGT TACCAGGCTG CTTCCATCTT CTTGATGAGT TGCCTGATAC AGTGTACCGT 
GTTTGTGATC TGATCATGAC AGCTATCAAA CGTAATGGAG CTGACTATCG TGATATGATT 
CTAAAGCAAG TTGTCAACCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCTCTAACAA CAAGTGACAC AAAAACAGTT TCAGAGTGGA TCAGTCAGAT GGCTACCCTG 
CCACAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CACTACTTTT TGAGGAGTTG 
AAGTTACCTT GTGCCTGGGT GGTTGAATCA AGTGGCATTC TTAATGTCCT AATCAAACTC 
TTAGAAGTGG TTCAACCCTG CCTACAGGCT GCCAAAGAGC AAAAGGAAGT CCAGACCCCA 
AAGTGGATCA CCCCAGTGTT GCTCCTTATT GATTTTTATG AAAAGACAGC CATCTCATCA 
AAGAGAAGAG CCCAAATGAC TAAGTACCTA CAGTCCAACA GCAACAACTG GCGCTGGTTT 
GATGACCGCT CTGGGCGTTG GTGTAGTTAC AGTGCTAGCA ACAACAGCAC CATTGATTCT 
GCCTGGAAAT CTGGAGAAAC AAGTGTAAGA TTCACTGCTG GCCGACGAAG ATACACTGTC 
CAGTTCACTA CAATGGTGCA GGTTAATGAA GAAACAGGAA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTGCCACG ACTGAGTAAG AACTCAAAAA GCAGCAATGG ACAGGAACTA 
GAGAAGACAC TGGAAGAAAG CAAGGAGACC GATATCAAAC ATAAAGAAAA TAAAGGCAAT 
GATATCCCCT TAGCTCTAGA GAGCACAAAC ACTGAAAAAG AGGCAAGCCT GGATGAAACA 
AAAATTGGGG AGATCCTAAT CCAGGGCCTG ACAGAAGATA TGGTGACTGT TCTAATCCGT 
GCCTGTGTGA GCATGCTGGG AGTCCCTGTG GACCCAGACA CTTTGCATGC CACCCTTCGT 
CTCTGTTTAA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACACGAATGA TCTTGAATTT GACCCAGAGC TCAGGTTTCA ATGGGTTTAC CCCCTTAGTC 
ACCCTTCTCT TAAGGCACAT CATTGAGGAT CCTTGTACTC TTCGTCATAC TATGGAAAAG 
GTTGTTCGCT CGGCAGCCAC AAGTGGAGCT GGTAGTACTA CATCTGGTGT TGTATCTGGC 
AGCCTCGGTT CTCGGGAGAT CAACTATATC CTTAGAGTCC TAGGACCAGC TGCCTGCCGC 
AATCCAGACA TATTCACAGA AGTAGCCAAT TGCTGTATCC GCATTGCCCT TCCAGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGTCCTAAT 
GCTGTACAGC TGGTAAAGAC AACACCTTTG AAGCCCTCAT CTCTGCCTGT CATTCCTGAT 
ACTATTAAGG AAGTTATCTA TGATATGCTG AATGCTTTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGACA AATCTGATCC TAAACCTGGG GGAACGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGACGATGT ATACCAGCAG TACCGGTCAC TTACTCGTCA GAGCAGTGAC 
TTTGATACAC AGTCAGGTTT TTCCTTAAAT AGTCAGGTTT TTGCTGCAGA TGGTGCCCCT 
GCTGAAACTT CCACAACTGG AACCTCCCAA GGAGAGGGAG CTTCAACTCC AGAGGAGACT 
CGAGAAGGGA AGAAAGATAA AGAAGGGGAC CGTACATCTG AGGAAGGCAA GCAGAAAAGC 
AAGGGCAGCA AACCTTTAAT GCCTACCTCT ACTATCCTTC GTCTTCTGGC AGAGTTGGTG 
AGGTCCTATG TTGGTATTGC TACCCTGATT GCAAACTACA GCTATACCGT GGGCCAGTCT 
GAGCTGATCA AAGAGGACTG CAGTGTGCTA GCTTTTGTCC TGGACCACCT CCTCCCACAC 
ACCCAGAATG CAGAAGACAA GGATACACCT GCCCTGGCAC GTCTATTCCT TGCAAGTCTG 
GCTGCTGCGG GGAGTGGCAC AGATGCTCAA GTGGCCCTAG TGAATGAAGT GAAAGCAGCC 
CTGGGACGGG CGCTTGCTAT GGCTGAGAGT ACAGAGAAGC ACGCCAGACT TCAGGCAGTG 
ATGTGTATCA TCAGTACTAT CATGGAATCC TGCCCCTCCA CCTCCAGCTT CTACAGTAGT 
GCTACAGCCA AGACTCAGCA TAATGGCATG AACAACATCA TTCGACTGTT CCTAAAGAAG 
GGACTAGTCA ATGACCTGGC CAGAGTTCCT CACAGCCTAG ACCTGTCCAG TCCCAATATG 
GCCAACACAG TCAATGCTGC TCTAAAACCC TTGGAAACAC TTTCCCGGAT TGTAAATCAA 
CCCAGTAGCC TTTTTGGCAG CAAGAGTGCT TCTAGCAAGA ACAAGTCTGA GCAGGATGCT 
CAAGGAGCTT CTCAGGACTC CAGCAGCCAT CAGCAAGATC CAGGCGAGCC TGGGGAGGCA 
GAAGTGCAGG AGGAGGATCA TGATGTCACT CAGACAGAGG TGGCAGATGG GGATATCATG 
GATGGGGAGG CTGAAACCGA CTCAGTGGTG ATTGCCGGGC AGCCTGAGGT GCTCAGTTCA 
CAAGAGATGC AGGTTGAAAA TGAACTGGAG GACCTGATAG ATGAGTTGCT TGAGAGGGAT 
GGCGGATCTG GGAACAGTAC AATTATAGTG AGCAGAAGTG GAGAGGATGA ATCACAAGAG 
GACGTGCTGA TGGATGAAGC TCCTTCCAAC CTCAGCCAAG CTTCCACCTT GCAGGCCAAC 
CGAGAAGATT CCATGAATAT CCTGGACCCT GAGGATGAGG AGGAACATAC TCAGGAAGAG 
GACAGCAGTG GCAGTAACGA GGATGAGGAT GATAGTCAGG ATGAAGAGGA GGAGGAGGAG 
GAAGATGAGG AAGATGATCA GGAGGATGAT GAAGGTGAAG AAGGAGATGA AGATGATGAC 
GACGATGGTT CTGAGATGGA ATTGGATGAG GATTACCCTG ATATGAACGC TTCTCCCTTG 
GTCCGATTTG AGCGCTTTGA CCGGGAGGAT GATCTCATCA TTGAGTTTGA CAACATGTTC 
TCCAGTGCTA CAGACATTCC CCCATCCCCA GGAAATATCC CTACCACCCA CCCATTGATG 
GTACGTCATG CAGATCACAG TTCCCTGACA CTGGGCAGTG GTTCTTCCAC AACTCGTCTT 
ACCCAGGGCA TCGGTCGCAG TCAAAGGACC CTCAGACAGC TCACTGCCAA TACAGGCCAT 
ACCATTCATG TTCACTACCC TGGGAATCGC CAGCCTAACC CTCCTCTTAT ACTCCAGAGG 
TTGCTTGGTC CCTCTGCAGC TGCTGACATT CTTCAGCTGA GCAGCAGCCT TCCATTACAA 
AGCCGAGGCC GGGCCCGCCT CCTAGTGGGC AATGATGACG TCCATATCAT CGCCAGGTCT 
GATGACGAGC TGCTGGATGA CTTTTTTCAT GACCAGAGTA CAGCCACTAG CCAAGCAGGA 
ACCCTGTCCA GCATCCCAAC AGCTCTAACC CGATGGACAG AGGAATGCAA AGTTCTTGAT 
GCTGAGAGTA TGCATGACTG TGTTTCAGTG GTTAAAGTGC CCATTGTCAA TCACCTGGAA 
TTCTTGAGAG ATGAGGAGCT AGAAGAGAGA AGGGAGAAAC GCAGGAAACA ATTGGCTGAG 
GAAGAAACAA AAATAATAGA CAAAGGCAAA GAAGACAAGG AGAACAGAGA TCAGAGTGCA 
CAGTGTACTG TAACTAAGAC AAATGACTCC ACTGAACAGA ATGTCTCAGA TGGGACTCCT 
ATGCCTGACA GCTATCCAAC AACCCCCTCT TCAACGGATG CACCTACATC TGAGTCCAAG 
GAAACACTTG GTACCCTACA ACCCTCCCAG CAACAGCCAG CACTTCCACC CCCACCATCC 
TTAGGAGAGA TTCCTCAGGA ACTACAGTCC CCTGCTGAAG AAGTGGCAAA CTCTACACAG 
CTATTGATGC CTATAGAGTT GGAGGAATTG GGTCCCACAA GACCAAGTGG AGAAGCAGAA 
ACAACTCAAA TGGAATTGTC TCCAGCTCCC ACCATAACCT CTCTTTCCCC AGAGAGAGCT 
GAAGATTCAG ACGCGTTAAC AGCTGTCAGC AGTCAGCTGG AAGGTTCTCC CATGGACACC 
AGCAGCCTGG CTTCCTGTAC GTTAGAAGAG GCTGTGGGTG ACACTCCAGC AGCTGGCAGT 
TCTGAGCAGC CCACAGCAGG TAGTTCCACT CCTGGGGATG CGCCATCAGT TGTGGCAGAA 
GTGCAAGGCA GACCTGATGT ATCAAGAGAA TCCAACCAGC CACCTGAGGA CAGCTCCCCC 
CCTGCATCCT CGGAGAGTTC TTCCACTAGA GATTCTGCCG TGGCCATCTC TGGAGCAGAT 
TCTCGTGGAA TTCTAGAAGA GCCACTACCT TCAACAAGCA GTGAAGAAGA AGATCCCCTT 
GCTGGTATCA GTCTCCCTGA AGGTGTGGAT CCCTCCTTTC TGGCTGCTCT CCCTGATGAT 
ATCCGTCGGG AAGTCCTACA GAACCAGCTG GGCATACGTC CACCAACCCG AAGTGCTCCC 
TCCTCAAATA GCTCAGCTCC TGCAGTAGTG GGGAACCCTG GTGTAACTGA AGTGAGCCCT 
GAGTTCCTGG CTGCCCTACC TCCAGCTATT CAAGAGGAAG TGCTAGCACA GCAGAGAGCT 
GAACAGCAGC GACGGGAACT GGCTCAGAAT GCGAGCTCAG ACACCCCCAT GGACCCTGTG 
ACTTTCATTC AGACTTTGCC CTCAGATCTG CGCCGTAGTG TCCTAGAGGA TATGGAAGAC 
AGTGTGTTAG CTGTGATGCC ACCTGACATT GCAGCTGAGG CTCAAGCTCT AAGACGAGAA 
CAAGAAGCCC GACAGCGGCA GCTCATGCAT GAGCGTCTTT TTGGACACAG TAGTACTTCT 
GCACTCTCTG CCATTCTCCG AAGCCCGGCT TTTACCAGTC GCCTGAGTGG CAACCGTGGG 
GTCCAGTATA CTCGCCTTGC TGTACAGAGG GGTGGCACCT TCCAGATGGG GGGTAGCAGC 
AGCCATAATA GGCCTTCTGG CAGTAATGTG GACACACTAC TCCGCCTCCG AGGACGACTC 
CTTCTAGACC ATGAAGCCCT GTCTTGCCTC TTGGTCCTAC TTTTTGTGGA TGAGCCAAAG 
CTCAATACTA GCCGTCTACA CCGAGTACTG AGAAATCTCT GCTATCATGC CCAGACCCGC 
CACTGGGTCA TCCGCAGCCT CCTGTCCATT TTGCAGCGCA GCAGTGAAAG TGAGTTATGC 
ATTGAGACAC CTAAGCTCTC CACAAGTGAA GAAAGGGGCA AGAAATCAAG CAAGAGCTGT 
GCATCAAGCA GTCATGAGAA CCGTCCCCTG GATCTGCTCC ACAAGATGGA GTCCAAAAGT 
TCCAATCAGC TCTCCTGGCT CTCAGTATCA ATGGATGCTG CTTTAGGCTG TAGGACTAAC 
ATATTCCAGA TTCAACGTTC TGGAGGGCGT AAACATACAG AGAAGCATGC AAGCAGTGGT 
TCCACTGTCC ACATCCACCC CCAGGCTGCT CCTGTTGTTT GCAGACATGT TTTGGATACA 
CTCATTCAGT TGGCCAAGGT GTTTCCCAGC CACTTTACAC AGCAGCGGAC CAAAGAAACA 
AACTGTGAGA GTGATCGGGA ACGGGGCAGT AAGCAGGCCT GCAGCCCATG CTCCTCACAG 
TCCTCCAGCA GTGGCATCTG TACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG 
AATGTTAGCC GAAAAGGCAA GAACTCCGTG AAGTCAGTAC CAGTGAGCTC TGGTGGTGAA 
GGGGAAACTT CCCCACACAG CCTTGAAGCC TCTCCACTGG GGCAGCTCAT GAACATGTTG 
TCACATCCAG TCATCCGCCG GAGTTCTCTC TTAACTGAGA AACTCCTGAG ACTCCTCTCT 
CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTATCAGAAG TGCAAACTAA CTCCAGCAAC 
AGTGGTTCCT CCACTGCTGC CACCTCAAAC ACATCTACTA CTACCACCAC CACCACTACT 
GCCACTGCCC CCACGCCCAC ACCCCCAGCT GCAACTACCC CTGTCACTTC TGCTCCAGCT 
CTGGTTGCTG CCACAGCTAT TTCCACCATT ACTGTAGCTG CTTCTACCAC AGTGACTACC 
CCCACAACTG CTACCACTAC TGTTTCAACT TCTACTACTA AAGGCAGCAA ATCTCCAGCA 
AAGGTTGGTG AAGGAGGCAG TGGTATAGAC TTCAAAATGG TGTCCTCTGG CCTCACTGAA 
AACCAGCTAC AGCTCTCTGT AGAGGTGTTG ACATCCCACT CTTGTTCTGA AGAAGGCTTG 
GAAGATGCGG CCAATGTGTT ACTGCAGCTC TCTCGTGGGG ACTCTGGTAC CCGGGACACT 
GTTCTTAAGC TGCTACTAAA TGGAGCCCGC CATCTGGGTT ATACCCTCTG TAAACAGATA 
GGTACCCTAC TCGCTGAACT ACGGGAATAT AATCTGGAGC AGCAGCGGCG AGCCCAATGC 
GAGACCCTCT CTCCTGATGG CCTACCTGAG GAGCAACCAC AGACCACCAA ACTGAAAGGC 
AAAATGCAGA GCAGGTTTGA CATGGCTGAA AATGTGGTAA TTGTGGCATC TCAGAAGCGA 
CCCTTGGGTG GCCGGGAGCT CCAGCTGCCT TCTATGTCCA TGTTGACATC CAAGACATCT 
ACCCAGAAGT TCTTCTTGAG GGTACTACAG GTCATCATCC AGCTGCGGGA TGACACACGA 
CGAGCTAACA AGAAAGCCAA GCAGACAGGC AGGCTAGGTT CCTCCGGTTT AGGCTCAGCT 
AGCAGCATCC AGGCAGCTGT TCGGCAGCTG GAGGCTGAGG CTGATGCCAT TATACAAATG 
GTACGTGAGG GTCAAAGGGC GCGGAGACAG CAACAAGCAG CAACGTCGGA GTCTTCTAAC 
CAGTCAGAGA CATCTGTCCG GAGAGAGGAA TCGCCCATGG ATGTGGACCA GCCATCTCCT 
AGTGCTCAAG ATACTCAGTC CATTGTAATC TCAGATGGAA CACCACAGGG AGAGAAGGAG 
AAAGAGGAGA AACCACCTGA GTTACCCCTG CTCAGTGAGC AACTGAGCCT AGATGAACTG 
TGGGACATGC TAGGGGAATG TTTAAAAGAA CTGGAGGAAT CTCATGACCA GCATGCAGTA 
TTAGTGCTAC AGCCTGCTGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC 
AAGCCTCCTG TCCGAGATAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA TGAACCTCCT 
CCACTCTCCC CCGCCCCCTT AACCCCAGCC ACTCCATCCT CCCTTGACCC ATTCTTCTCC 
AGGGAGCCCT CATCTATGCA CATTTCCTCG AGCCTGCCCC CTGACACACA GAAGTTCCTT 
CGCTTTGCAG AGACTCACCG CACTGTGTTA AACCAGATAC TACGGCAGTC TACCACCCAT 
CTTGCTGATG GGCCTTTTGC TGTCTTGGTA GATTATATCC GTGTCCTCGA CTTTGATGTC 
AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG CGTTTGGACG AAGGGCTCCG GAAAGAAGAT 
ATGGCAGTGC ATGTCCGCCG TGACCATGTA TTTGAAGACT CCTATCGTGA GCTGCATCGC 
AAATCTCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG 
GATGCTGGGG GGCTCCTGCG AGAGTGGTAC ATGATCATCT CTCGAGAGAT GTTCAACCCT 
ATGTATGCCT TGTTCCGTAC CTCACCTGGT GACCGGGTCA CCTACACCAT CAATCCATCT 
TCCCACTGTA ACCCCAACCA CCTCAGCTAC TTCAAGTTTG TTGGACGAAT TGTAGCCAAA 
GCTGTATATG ACAACCGCCT CCTGGAGTGC TACTTTACTA GGTCTTTCTA CAAACACATC 
TTGGGCAAAT CTGTCAGGTA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC 
CTAGTTTATC TGCTTGAAAA TGATGTCTCT ACATTAGGCT ATGACCTCAC CTTCAGCACT 
GAGGTTCAAG AATTTGGAGT ATGTGAAGTT CGTGACCTCA AACCCAATGG AGCCAACATC 
TTGGTAACAG AGGAGAACAA GAAGGAATAT GTACACCTGG TTTGCCAGAT GAGAATGACA 
GGAGCCATCC GCAAACAGCT GGCAGCCTTC TTGGAGGGCT TCTATGAAAT CATTCCAAAG 
CGCCTCATAT CCATCTTTAC TGAGCAGGAA TTAGAGCTGC TCATATCAGG GCTGCCTACC 
ATCGACATTG ATGATCTAAA ATCTAACACT GAGTACCACA AGTACCAATC CAACTCTATT 
CAGATCCAGT GGTTTTGGAG AGCATTGCGT TCCTTTGACC AAGCAGACCG TGCCAAGTTC 
CTCCAGTTTG TCACAGGTAC TTCCAAGGTA CCCCTACAAG GATTTGCTGC CCTTGAAGGT 
ATGAATGGCA TTCAGAAGTT TCAGATTCAT CGAGATGACA GATCCACAGA TCGCCTGCCT 
TCAGCTCACA CATGTTTTAA TCAACTGGAC CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC 
CGCCACATGC TACTATTGGC CATCCAGGAA TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528934.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528997.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X1, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528934.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006528934.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528997.1
CDS	1191..14561
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528934.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528934.2

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528936.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528999.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X3, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528936.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006528936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528999.1
CDS	1144..14514
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528936.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528936.2

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528939.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529002.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X7, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528939.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006528939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529002.1
CDS	1092..14462
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528939.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528939.2

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_011247852.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246154.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X9, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_011247852.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246154.1
CDS	655..14025
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_011247852.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247852.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_017318587.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174076.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X10, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_017318587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174076.1
CDS	1179..14549
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017318587.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318587.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_011247853.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246155.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X11, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_011247853.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246155.1
CDS	1030..14400
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_011247853.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247853.2

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528934.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528997.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X2, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528934.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528934.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528997.1
CDS	1213..14583
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528934.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528934.3

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251436.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107296.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X6, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_030251436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107296.1
CDS	1162..14532
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251436.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251436.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528938.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529001.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X8, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528938.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528938.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529001.1
CDS	1118..14488
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528938.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528938.3

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251442.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107302.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X18, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_030251442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107302.1
CDS	238..13608
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251442.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251442.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528937.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529000.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X5, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528937.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006528937.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529000.1
CDS	1049..14419
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528937.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528937.2

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528938.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529001.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X6, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528938.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006528938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529001.1
CDS	1096..14466
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528938.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528938.2

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251435.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107295.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X4, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_030251435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107295.1
CDS	1852..15222
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251435.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251435.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528936.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528999.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X5, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528936.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528936.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528999.1
CDS	1166..14536
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528936.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528936.3

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251437.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107297.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X12, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_030251437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107297.1
CDS	673..14043
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251437.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251437.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528940.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529003.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X8, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528940.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006528940.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529003.1
CDS	1045..14415
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528940.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528940.2

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528943.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529006.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X12, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528943.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006528943.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529006.1
CDS	519..13889
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528943.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528943.2

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528933.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528996.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X3, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528933.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528933.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528996.1
CDS	935..14305
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528933.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528933.3

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528937.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529000.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X7, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528937.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528937.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529000.1
CDS	1071..14441
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528937.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528937.3

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_011247852.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246154.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X11, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_011247852.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246154.1
CDS	677..14047
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_011247852.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247852.2

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251439.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107299.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X14, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_030251439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107299.1
CDS	1673..15043
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251439.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251439.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251441.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107301.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X17, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_030251441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107301.1
CDS	535..13905
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251441.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251441.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528939.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529002.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X9, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528939.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528939.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529002.1
CDS	1114..14484
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528939.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528939.3

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528943.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529006.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X16, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528943.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528943.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529006.1
CDS	541..13911
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528943.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528943.3

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528935.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528998.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X2, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528935.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006528935.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528998.1
CDS	1187..14557
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528935.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528935.2

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528933.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528996.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X4, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528933.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006528933.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528996.1
CDS	913..14283
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528933.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528933.2

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251434.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107294.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X1, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_030251434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107294.1
CDS	1856..15226
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251434.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251434.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528940.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529003.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X10, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_006528940.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528940.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529003.1
CDS	1067..14437
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528940.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528940.3

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251438.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107298.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X13, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_030251438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107298.1
CDS	1179..14549
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251438.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251438.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251440.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107300.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X15, mRNA.

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CloneID	OMu56714
Clone ID Related Accession (Same CDS sequence)	XM_006528934.2 , XM_006528936.2 , XM_006528939.2 , XM_011247852.1 , XM_017318587.1 , XM_011247853.2 , XM_006528934.3 , XM_030251436.1 , XM_006528938.3 , XM_030251442.1 , XM_006528937.2 , XM_006528938.2 , XM_030251435.1 , XM_006528936.3 , XM_030251437.1 , XM_006528940.2 , XM_006528943.2 , XM_006528933.3 , XM_006528937.3 , XM_011247852.2 , XM_030251439.1 , XM_030251441.1 , XM_006528939.3 , XM_006528943.3 , XM_006528935.2 , XM_006528933.2 , XM_030251434.1 , XM_006528940.3 , XM_030251438.1 , XM_030251440.1
Accession Version	XM_030251440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107300.1
CDS	1041..14411
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251440.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251440.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTTC TAACCAGTCA 
GAGACATCTG TCCGGAGAGA GGAATCGCCC ATGGATGTGG ACCAGCCATC TCCTAGTGCT 
CAAGATACTC AGTCCATTGT AATCTCAGAT GGAACACCAC AGGGAGAGAA GGAGAAAGAG 
GAGAAACCAC CTGAGTTACC CCTGCTCAGT GAGCAACTGA GCCTAGATGA ACTGTGGGAC 
ATGCTAGGGG AATGTTTAAA AGAACTGGAG GAATCTCATG ACCAGCATGC AGTATTAGTG 
CTACAGCCTG CTGTCGAGGC CTTCTTTTTG GTCCATGCCA CAGAGCGGGA GAGCAAGCCT 
CCTGTCCGAG ATACCCGTGA GAGCCAGCTG GCACACATCA AGGATGAACC TCCTCCACTC 
TCCCCCGCCC CCTTAACCCC AGCCACTCCA TCCTCCCTTG ACCCATTCTT CTCCAGGGAG 
CCCTCATCTA TGCACATTTC CTCGAGCCTG CCCCCTGACA CACAGAAGTT CCTTCGCTTT 
GCAGAGACTC ACCGCACTGT GTTAAACCAG ATACTACGGC AGTCTACCAC CCATCTTGCT 
GATGGGCCTT TTGCTGTCTT GGTAGATTAT ATCCGTGTCC TCGACTTTGA TGTCAAGCGC 
AAATATTTCC GCCAAGAGCT GGAGCGTTTG GACGAAGGGC TCCGGAAAGA AGATATGGCA 
GTGCATGTCC GCCGTGACCA TGTATTTGAA GACTCCTATC GTGAGCTGCA TCGCAAATCT 
CCTGAAGAAA TGAAGAATCG ATTGTATATA GTGTTTGAAG GAGAAGAAGG GCAGGATGCT 
GGGGGGCTCC TGCGAGAGTG GTACATGATC ATCTCTCGAG AGATGTTCAA CCCTATGTAT 
GCCTTGTTCC GTACCTCACC TGGTGACCGG GTCACCTACA CCATCAATCC ATCTTCCCAC 
TGTAACCCCA ACCACCTCAG CTACTTCAAG TTTGTTGGAC GAATTGTAGC CAAAGCTGTA 
TATGACAACC GCCTCCTGGA GTGCTACTTT ACTAGGTCTT TCTACAAACA CATCTTGGGC 
AAATCTGTCA GGTATACAGA TATGGAGAGT GAAGATTACC ACTTCTACCA GGGCCTAGTT 
TATCTGCTTG AAAATGATGT CTCTACATTA GGCTATGACC TCACCTTCAG CACTGAGGTT 
CAAGAATTTG GAGTATGTGA AGTTCGTGAC CTCAAACCCA ATGGAGCCAA CATCTTGGTA 
ACAGAGGAGA ACAAGAAGGA ATATGTACAC CTGGTTTGCC AGATGAGAAT GACAGGAGCC 
ATCCGCAAAC AGCTGGCAGC CTTCTTGGAG GGCTTCTATG AAATCATTCC AAAGCGCCTC 
ATATCCATCT TTACTGAGCA GGAATTAGAG CTGCTCATAT CAGGGCTGCC TACCATCGAC 
ATTGATGATC TAAAATCTAA CACTGAGTAC CACAAGTACC AATCCAACTC TATTCAGATC 
CAGTGGTTTT GGAGAGCATT GCGTTCCTTT GACCAAGCAG ACCGTGCCAA GTTCCTCCAG 
TTTGTCACAG GTACTTCCAA GGTACCCCTA CAAGGATTTG CTGCCCTTGA AGGTATGAAT 
GGCATTCAGA AGTTTCAGAT TCATCGAGAT GACAGATCCA CAGATCGCCT GCCTTCAGCT 
CACACATGTT TTAATCAACT GGACCTGCCT GCCTATGAGA GCTTTGAGAA GCTCCGCCAC 
ATGCTACTAT TGGCCATCCA GGAATGCTCT GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251451.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107311.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X28, mRNA.

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CloneID	OMu56719
Clone ID Related Accession (Same CDS sequence)	XM_030251451.1 , XM_030251452.1 , XM_030251448.1 , XM_030251449.1 , XM_006528948.2 , XM_006528948.3 , XM_030251450.1 , XM_030251453.1 , XM_030251454.1
Accession Version	XM_030251451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107311.1
CDS	1003..14151
Translation	MSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251451.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251451.1

ORF Insert Sequence:

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251452.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107312.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X29, mRNA.

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CloneID	OMu56719
Clone ID Related Accession (Same CDS sequence)	XM_030251451.1 , XM_030251452.1 , XM_030251448.1 , XM_030251449.1 , XM_006528948.2 , XM_006528948.3 , XM_030251450.1 , XM_030251453.1 , XM_030251454.1
Accession Version	XM_030251452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107312.1
CDS	871..14019
Translation	MSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251452.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251452.1

ORF Insert Sequence:

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251448.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107308.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X24, mRNA.

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CloneID	OMu56719
Clone ID Related Accession (Same CDS sequence)	XM_030251451.1 , XM_030251452.1 , XM_030251448.1 , XM_030251449.1 , XM_006528948.2 , XM_006528948.3 , XM_030251450.1 , XM_030251453.1 , XM_030251454.1
Accession Version	XM_030251448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107308.1
CDS	2186..15334
Translation	MSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251448.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251448.1

ORF Insert Sequence:

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251449.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107309.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X26, mRNA.

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CloneID	OMu56719
Clone ID Related Accession (Same CDS sequence)	XM_030251451.1 , XM_030251452.1 , XM_030251448.1 , XM_030251449.1 , XM_006528948.2 , XM_006528948.3 , XM_030251450.1 , XM_030251453.1 , XM_030251454.1
Accession Version	XM_030251449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107309.1
CDS	1444..14592
Translation	MSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251449.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251449.1

ORF Insert Sequence:

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528948.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529011.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X17, mRNA.

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CloneID	OMu56719
Clone ID Related Accession (Same CDS sequence)	XM_030251451.1 , XM_030251452.1 , XM_030251448.1 , XM_030251449.1 , XM_006528948.2 , XM_006528948.3 , XM_030251450.1 , XM_030251453.1 , XM_030251454.1
Accession Version	XM_006528948.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006528948.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529011.1
CDS	1243..14391
Translation	MSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528948.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528948.2

ORF Insert Sequence:

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528948.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529011.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X25, mRNA.

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CloneID	OMu56719
Clone ID Related Accession (Same CDS sequence)	XM_030251451.1 , XM_030251452.1 , XM_030251448.1 , XM_030251449.1 , XM_006528948.2 , XM_006528948.3 , XM_030251450.1 , XM_030251453.1 , XM_030251454.1
Accession Version	XM_006528948.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528948.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529011.1
CDS	1265..14413
Translation	MSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528948.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528948.3

ORF Insert Sequence:

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251450.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107310.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X27, mRNA.

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CloneID	OMu56719
Clone ID Related Accession (Same CDS sequence)	XM_030251451.1 , XM_030251452.1 , XM_030251448.1 , XM_030251449.1 , XM_006528948.2 , XM_006528948.3 , XM_030251450.1 , XM_030251453.1 , XM_030251454.1
Accession Version	XM_030251450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107310.1
CDS	1007..14155
Translation	MSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251450.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251450.1

ORF Insert Sequence:

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251453.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107313.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X30, mRNA.

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CloneID	OMu56719
Clone ID Related Accession (Same CDS sequence)	XM_030251451.1 , XM_030251452.1 , XM_030251448.1 , XM_030251449.1 , XM_006528948.2 , XM_006528948.3 , XM_030251450.1 , XM_030251453.1 , XM_030251454.1
Accession Version	XM_030251453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107313.1
CDS	977..14125
Translation	MSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251453.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251453.1

ORF Insert Sequence:

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251454.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107314.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X31, mRNA.

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CloneID	OMu56719
Clone ID Related Accession (Same CDS sequence)	XM_030251451.1 , XM_030251452.1 , XM_030251448.1 , XM_030251449.1 , XM_006528948.2 , XM_006528948.3 , XM_030251450.1 , XM_030251453.1 , XM_030251454.1
Accession Version	XM_030251454.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107314.1
CDS	608..13756
Translation	MSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251454.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251454.1

ORF Insert Sequence:

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGTTG CAGATGTAAC TATGGAAAGC AGTCCAGGCT CGTCCATATC TATGGAGCAC 
CGGCTGGATG TTGAATTAAG GGCATCAAGT TCCAACAGCA GCACTAGCAT CTCTGGCCCT 
GGCCCGGGTC CTGGCCCTGG TCCGGGTCCT GGCCCTGGTC CTGGCCCTGG TCCTGGTCCT 
GGCCCTGGCC CTGGCCCTGG CCCCGGCCCT GGCCCTGGTC CTGGCCCTGG ACCCCGTCCT 
GGAGTTCAGT GTATTCCACA ACGAGCAGCA CTTCTCAAGT CCATGTTGAA TTTCCTCAAG 
AAGGCCATTC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTTTA 
CCTACCTCTC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGTCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAGC CATCACTGCT GTCCTCACTC 
CAGGACAATG GACTGACAGA TGTCATGCTG CATGCACTGC TCATCAAAGA TGTTCCTGCT 
ACAAGGGAAG TCCTCGGTTC CCTCCCAAAT GTATTCAGTG CACTTTGCTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTACA ATGTCAGCCA TTTGAACGTC TCTTCAAAGT TCTTCTGTCT 
CCTGATTACC TCCCAGCCAT GCGGAGGAGG AGGAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT TGATGAGCTC ATGAGACACC AGCCTACCCT CAAAACAGAT 
GCAACAACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG AGATCCTAAA 
TACATTTGTC AGAAGCCATC TATTCAGAAA GCGGATGGTA CTGCTACTGC TCCTCCTCCA 
AGGTCTAATC ATGCTGCAGA AGAGGCTTCC AGTGAAGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TGCCCAGCAG AATGAAACTG AGCCTAATCA GCAGGTCGTT 
GGTACCGAGG AACGTATACC TATACCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTTTGAGCAA CAATACAACA GATGACCACT GTCAAGAATT TGTAAACCAG 
AAAGGACTCT TACCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC CCTGTCACAT 
GAACCCAAAG TTCTTCAAGA AGGTCTCCTT CAATTAGACT TGATCCTTTC CTCCTTGGAG 
CCTTTACATC GGCCGATTGA ATCCCCTGGG GGCTCAGTGT TACTGAGAGA ATTGGCATGT 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCTCAGGCCA CGCCTCTGTT GCATGCACTC 
ACTGCAGCCC ATGCTTACAT CATGATGTTT GTTCACACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCTA TCTCAGTAAA CCAGTGGGGT TCACAATTGG GTTTGAGTGT TCTGAGCAAG 
CTAAGCCAGT TATACTGTTC TCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTATGTACC 
CCCAACAGCC TACCATCTGG ATGTGAATTT GGTCAAGCGG ACATGCAGAA ACTAGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGAGA ACAGGATGGA 
ACTGCTGGAA GCATGGATGC ATCTGCCCAG GGTTTATTAG AAGGAATTGA ACTAGATGGT 
GACACACTGG CACCCATGGA GACAGATGAA CCTTCTTCTT CAGATTCTAA GGGCAAATCT 
AAAATCACAC CTGCCATGGC TGCAAGAATT AAACAAATCA AGCCGTTGCT GTCAGCTTCT 
TCCAGATTAG GCCGAGCACT TGCTGAGCTC TTTGGACTCC TTGTTAAGCT TTGTGTGGGT 
TCTCCTGTCC GCCAAAGGAG GAGCCATCAT GCTGCCAGTA CCACCACTGC ACCAACACCT 
GCTGCTAGAT CAACAGCTTC AGCCCTCACT AAGCTACTCA CAAAAGGCTT ATCATGGCAG 
CCCCCACCAT ATACACCCAC TCCCCGCTTC AGGCTGACGT TCTTCATCTG TTCAGTTGGA 
TTCACCTCTC CAATGCTCTT TGATGAGAGA AAGTATCCTT ACCACCTTAT GCTGCAGAAA 
TTTCTCTGCT CTGGTGGTCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTATCCATG 
GGAGGAAAAG TTCCTGTTTC TGAGGGATTG GAACATTCAG ATCTACCTGA TGGTACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTTGTA GAAAAGATGG TAAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGTTGCC TGCTAAATTG CCTGGAGGAG TCCAGAGCTT TCCACAATTT 
AGTGCCCTTC GCTTCCTTGT AGTAACTCAA AAAGCAGCCT TTACTTGTAT CAAGAACCTG 
TGGAACCGGA AACCCCTTAA AGTATATGGG GGAAGAATGG CTGAGTCTAT GCTGGCCATT 
TTGTGCCACA TTCTCCGAGG GGAACCTGTG ATTCGAGAAA GACTGAGCAA AGAGAAGGAA 
GGATCTCGAG GAGAAGAAGA AGCAGGCCAG GAAGAAGGTG GCTCACGCAG AGAACCTCAA 
GTCAACCAGC AACAGCTACA ACAGCTCATG GACATGGGTT TCACAAGAGA ACATGCCATG 
GAGGCACTGT TGAATACCAG TACGATGGAA CAAGCAACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TAGGAGGAGT TGTTCGGGAT CTTAGCATGT CAGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAA GAGCGTAAAG CTCGAGAAAA ACAAGAAGAG 
GAAGAAGCTA AATGTCTAGA GAAGTTTCAA GATGCTGACC CCTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACAAT GTTACCAGGC TGCTTCCATC TTCTTGATGA GTTGCCTGAT 
ACAGTGTACC GTGTTTGTGA TCTGATCATG ACAGCTATCA AACGTAATGG AGCTGACTAT 
CGTGATATGA TTCTAAAGCA AGTTGTCAAC CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCTCTAAC AACAAGTGAC ACAAAAACAG TTTCAGAGTG GATCAGTCAG 
ATGGCTACCC TGCCACAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACACTACTT 
TTTGAGGAGT TGAAGTTACC TTGTGCCTGG GTGGTTGAAT CAAGTGGCAT TCTTAATGTC 
CTAATCAAAC TCTTAGAAGT GGTTCAACCC TGCCTACAGG CTGCCAAAGA GCAAAAGGAA 
GTCCAGACCC CAAAGTGGAT CACCCCAGTG TTGCTCCTTA TTGATTTTTA TGAAAAGACA 
GCCATCTCAT CAAAGAGAAG AGCCCAAATG ACTAAGTACC TACAGTCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGACCG CTCTGGGCGT TGGTGTAGTT ACAGTGCTAG CAACAACAGC 
ACCATTGATT CTGCCTGGAA ATCTGGAGAA ACAAGTGTAA GATTCACTGC TGGCCGACGA 
AGATACACTG TCCAGTTCAC TACAATGGTG CAGGTTAATG AAGAAACAGG AAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTGCCA CGACTGAGTA AGAACTCAAA AAGCAGCAAT 
GGACAGGAAC TAGAGAAGAC ACTGGAAGAA AGCAAGGAGA CCGATATCAA ACATAAAGAA 
AATAAAGGCA ATGATATCCC CTTAGCTCTA GAGAGCACAA ACACTGAAAA AGAGGCAAGC 
CTGGATGAAA CAAAAATTGG GGAGATCCTA ATCCAGGGCC TGACAGAAGA TATGGTGACT 
GTTCTAATCC GTGCCTGTGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA CACTTTGCAT 
GCCACCCTTC GTCTCTGTTT AAGGCTCACC CGAGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACACGAAT GATCTTGAAT TTGACCCAGA GCTCAGGTTT CAATGGGTTT 
ACCCCCTTAG TCACCCTTCT CTTAAGGCAC ATCATTGAGG ATCCTTGTAC TCTTCGTCAT 
ACTATGGAAA AGGTTGTTCG CTCGGCAGCC ACAAGTGGAG CTGGTAGTAC TACATCTGGT 
GTTGTATCTG GCAGCCTCGG TTCTCGGGAG ATCAACTATA TCCTTAGAGT CCTAGGACCA 
GCTGCCTGCC GCAATCCAGA CATATTCACA GAAGTAGCCA ATTGCTGTAT CCGCATTGCC 
CTTCCAGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGTCCTA ATGCTGTACA GCTGGTAAAG ACAACACCTT TGAAGCCCTC ATCTCTGCCT 
GTCATTCCTG ATACTATTAA GGAAGTTATC TATGATATGC TGAATGCTTT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA CAAATCTGAT CCTAAACCTG GGGGAACGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGACGAT GTATACCAGC AGTACCGGTC ACTTACTCGT 
CAGAGCAGTG ACTTTGATAC ACAGTCAGGT TTTTCCTTAA ATAGTCAGGT TTTTGCTGCA 
GATGGTGCCC CTGCTGAAAC TTCCACAACT GGAACCTCCC AAGGAGAGGG AGCTTCAACT 
CCAGAGGAGA CTCGAGAAGG GAAGAAAGAT AAAGAAGGGG ACCGTACATC TGAGGAAGGC 
AAGCAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCTACCT CTACTATCCT TCGTCTTCTG 
GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCAAACTA CAGCTATACC 
GTGGGCCAGT CTGAGCTGAT CAAAGAGGAC TGCAGTGTGC TAGCTTTTGT CCTGGACCAC 
CTCCTCCCAC ACACCCAGAA TGCAGAAGAC AAGGATACAC CTGCCCTGGC ACGTCTATTC 
CTTGCAAGTC TGGCTGCTGC GGGGAGTGGC ACAGATGCTC AAGTGGCCCT AGTGAATGAA 
GTGAAAGCAG CCCTGGGACG GGCGCTTGCT ATGGCTGAGA GTACAGAGAA GCACGCCAGA 
CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAAT CCTGCCCCTC CACCTCCAGC 
TTCTACAGTA GTGCTACAGC CAAGACTCAG CATAATGGCA TGAACAACAT CATTCGACTG 
TTCCTAAAGA AGGGACTAGT CAATGACCTG GCCAGAGTTC CTCACAGCCT AGACCTGTCC 
AGTCCCAATA TGGCCAACAC AGTCAATGCT GCTCTAAAAC CCTTGGAAAC ACTTTCCCGG 
ATTGTAAATC AACCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAACAAGTCT 
GAGCAGGATG CTCAAGGAGC TTCTCAGGAC TCCAGCAGCC ATCAGCAAGA TCCAGGCGAG 
CCTGGGGAGG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT 
GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCCGG GCAGCCTGAG 
GTGCTCAGTT CACAAGAGAT GCAGGTTGAA AATGAACTGG AGGACCTGAT AGATGAGTTG 
CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT 
GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC 
TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAACAT 
ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG 
GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAAGGAGAT 
GAAGATGATG ACGACGATGG TTCTGAGATG GAATTGGATG AGGATTACCC TGATATGAAC 
GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT 
GACAACATGT TCTCCAGTGC TACAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528951.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529014.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X37, mRNA.

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CloneID	OMu56721
Clone ID Related Accession (Same CDS sequence)	XM_006528951.3 , XM_006528951.2
Accession Version	XM_006528951.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGGAG TTCAGTGTAT TCCACAACGA GCAGCACTTC TCAAGTCCAT GTTGAATTTC 
CTCAAGAAGG CCATTCAAGA CCCTGCTTTC TCAGATGGCA TACGACATGT GATGGATGGT 
TCTTTACCTA CCTCTCTGAA ACACATCATC AGCAATGCAG AATACTATGG TCCATCACTC 
TTCCTCCTAG CTACTGAAGT GGTGACTGTG TTTGTATTTC AAGAGCCATC ACTGCTGTCC 
TCACTCCAGG ACAATGGACT GACAGATGTC ATGCTGCATG CACTGCTCAT CAAAGATGTT 
CCTGCTACAA GGGAAGTCCT CGGTTCCCTC CCAAATGTAT TCAGTGCACT TTGCTTGAAT 
GCCCGAGGTC TTCAGTCTTT TGTACAATGT CAGCCATTTG AACGTCTCTT CAAAGTTCTT 
CTGTCTCCTG ATTACCTCCC AGCCATGCGG AGGAGGAGGA GTTCTGATCC CCTTGGGGAT 
ACTGCATCCA ACCTGGGGAG TGCTGTTGAT GAGCTCATGA GACACCAGCC TACCCTCAAA 
ACAGATGCAA CAACTGCCAT CATCAAGTTA CTTGAAGAAA TCTGTAATCT TGGAAGAGAT 
CCTAAATACA TTTGTCAGAA GCCATCTATT CAGAAAGCGG ATGGTACTGC TACTGCTCCT 
CCTCCAAGGT CTAATCATGC TGCAGAAGAG GCTTCCAGTG AAGATGAGGA GGAAGAGGAA 
GTACAGGCCA TGCAGAGCTT TAATTCTGCC CAGCAGAATG AAACTGAGCC TAATCAGCAG 
GTCGTTGGTA CCGAGGAACG TATACCTATA CCCCTCATGG ATTACATCCT TAATGTGATG 
AAATTTGTGG AATCTATTTT GAGCAACAAT ACAACAGATG ACCACTGTCA AGAATTTGTA 
AACCAGAAAG GACTCTTACC TTTGGTTACC ATTTTGGGTC TTCCCAATCT GCCCATTGAC 
TTTCCCACAT CTGCTGCCTG TCAGGCTGTT GCAGGTGTCT GCAAATCCAT ATTGACCCTG 
TCACATGAAC CCAAAGTTCT TCAAGAAGGT CTCCTTCAAT TAGACTTGAT CCTTTCCTCC 
TTGGAGCCTT TACATCGGCC GATTGAATCC CCTGGGGGCT CAGTGTTACT GAGAGAATTG 
GCATGTGCAG GCAATGTTGC TGATGCTACC CTCTCAGCTC AGGCCACGCC TCTGTTGCAT 
GCACTCACTG CAGCCCATGC TTACATCATG ATGTTTGTTC ACACTTGCAG AGTTGGACAG 
AGTGAAATTC GTTCTATCTC AGTAAACCAG TGGGGTTCAC AATTGGGTTT GAGTGTTCTG 
AGCAAGCTAA GCCAGTTATA CTGTTCTCTG GTGTGGGAAA GCACTGTCCT CCTCTCTCTA 
TGTACCCCCA ACAGCCTACC ATCTGGATGT GAATTTGGTC AAGCGGACAT GCAGAAACTA 
GTTCCAAAGG ATGAGAAGGC AGGTACGACC CAGGGCGGAA AAAGATCAGA TGGAGAACAG 
GATGGAACTG CTGGAAGCAT GGATGCATCT GCCCAGGGTT TATTAGAAGG AATTGAACTA 
GATGGTGACA CACTGGCACC CATGGAGACA GATGAACCTT CTTCTTCAGA TTCTAAGGGC 
AAATCTAAAA TCACACCTGC CATGGCTGCA AGAATTAAAC AAATCAAGCC GTTGCTGTCA 
GCTTCTTCCA GATTAGGCCG AGCACTTGCT GAGCTCTTTG GACTCCTTGT TAAGCTTTGT 
GTGGGTTCTC CTGTCCGCCA AAGGAGGAGC CATCATGCTG CCAGTACCAC CACTGCACCA 
ACACCTGCTG CTAGATCAAC AGCTTCAGCC CTCACTAAGC TACTCACAAA AGGCTTATCA 
TGGCAGCCCC CACCATATAC ACCCACTCCC CGCTTCAGGC TGACGTTCTT CATCTGTTCA 
GTTGGATTCA CCTCTCCAAT GCTCTTTGAT GAGAGAAAGT ATCCTTACCA CCTTATGCTG 
CAGAAATTTC TCTGCTCTGG TGGTCACAAT GCTCTTTTTG AAACTTTCAA CTGGGCTCTA 
TCCATGGGAG GAAAAGTTCC TGTTTCTGAG GGATTGGAAC ATTCAGATCT ACCTGATGGT 
ACAGGAGAAT TCCTAGATGC CTGGCTTATG CTTGTAGAAA AGATGGTAAA TCCCACCACG 
GTGCTTGAAT CTCCACATTC GTTGCCTGCT AAATTGCCTG GAGGAGTCCA GAGCTTTCCA 
CAATTTAGTG CCCTTCGCTT CCTTGTAGTA ACTCAAAAAG CAGCCTTTAC TTGTATCAAG 
AACCTGTGGA ACCGGAAACC CCTTAAAGTA TATGGGGGAA GAATGGCTGA GTCTATGCTG 
GCCATTTTGT GCCACATTCT CCGAGGGGAA CCTGTGATTC GAGAAAGACT GAGCAAAGAG 
AAGGAAGGAT CTCGAGGAGA AGAAGAAGCA GGCCAGGAAG AAGGTGGCTC ACGCAGAGAA 
CCTCAAGTCA ACCAGCAACA GCTACAACAG CTCATGGACA TGGGTTTCAC AAGAGAACAT 
GCCATGGAGG CACTGTTGAA TACCAGTACG ATGGAACAAG CAACAGAGTA CCTTTTAACC 
CACCCTCCTC CAATCATAGG AGGAGTTGTT CGGGATCTTA GCATGTCAGA AGAGGACCAG 
ATGATGAGAG CAATTGCTAT GTCTCTGGGA CAGGATATTC CAATGGATCA AAGGGCAGAG 
TCACCTGAGG AAGTTGCTTG CCGGAAGGAG GAAGAAGAGC GTAAAGCTCG AGAAAAACAA 
GAAGAGGAAG AAGCTAAATG TCTAGAGAAG TTTCAAGATG CTGACCCCTT GGAACAAGAT 
GAGCTCCACA CTTTCACAGA TACAATGTTA CCAGGCTGCT TCCATCTTCT TGATGAGTTG 
CCTGATACAG TGTACCGTGT TTGTGATCTG ATCATGACAG CTATCAAACG TAATGGAGCT 
GACTATCGTG ATATGATTCT AAAGCAAGTT GTCAACCAGG TGTGGGAAGC TGCTGATGTA 
TTGATCAAAG CTGCTCTTCC TCTAACAACA AGTGACACAA AAACAGTTTC AGAGTGGATC 
AGTCAGATGG CTACCCTGCC ACAGGCCTCC AATTTGGCTA CTAGAATCTT GCTTTTAACA 
CTACTTTTTG AGGAGTTGAA GTTACCTTGT GCCTGGGTGG TTGAATCAAG TGGCATTCTT 
AATGTCCTAA TCAAACTCTT AGAAGTGGTT CAACCCTGCC TACAGGCTGC CAAAGAGCAA 
AAGGAAGTCC AGACCCCAAA GTGGATCACC CCAGTGTTGC TCCTTATTGA TTTTTATGAA 
AAGACAGCCA TCTCATCAAA GAGAAGAGCC CAAATGACTA AGTACCTACA GTCCAACAGC 
AACAACTGGC GCTGGTTTGA TGACCGCTCT GGGCGTTGGT GTAGTTACAG TGCTAGCAAC 
AACAGCACCA TTGATTCTGC CTGGAAATCT GGAGAAACAA GTGTAAGATT CACTGCTGGC 
CGACGAAGAT ACACTGTCCA GTTCACTACA ATGGTGCAGG TTAATGAAGA AACAGGAAAC 
CGACGCCCTG TGATGCTGAC TCTCCTCAGG GTGCCACGAC TGAGTAAGAA CTCAAAAAGC 
AGCAATGGAC AGGAACTAGA GAAGACACTG GAAGAAAGCA AGGAGACCGA TATCAAACAT 
AAAGAAAATA AAGGCAATGA TATCCCCTTA GCTCTAGAGA GCACAAACAC TGAAAAAGAG 
GCAAGCCTGG ATGAAACAAA AATTGGGGAG ATCCTAATCC AGGGCCTGAC AGAAGATATG 
GTGACTGTTC TAATCCGTGC CTGTGTGAGC ATGCTGGGAG TCCCTGTGGA CCCAGACACT 
TTGCATGCCA CCCTTCGTCT CTGTTTAAGG CTCACCCGAG ACCACAAATA TGCCATGATG 
TTTGCAGAAC TGAAGAGTAC ACGAATGATC TTGAATTTGA CCCAGAGCTC AGGTTTCAAT 
GGGTTTACCC CCTTAGTCAC CCTTCTCTTA AGGCACATCA TTGAGGATCC TTGTACTCTT 
CGTCATACTA TGGAAAAGGT TGTTCGCTCG GCAGCCACAA GTGGAGCTGG TAGTACTACA 
TCTGGTGTTG TATCTGGCAG CCTCGGTTCT CGGGAGATCA ACTATATCCT TAGAGTCCTA 
GGACCAGCTG CCTGCCGCAA TCCAGACATA TTCACAGAAG TAGCCAATTG CTGTATCCGC 
ATTGCCCTTC CAGCCCCTCG AGGCTCAGGA ACTGCTTCAG ATGATGAATT TGAGAATCTT 
AGAATTAAAG GTCCTAATGC TGTACAGCTG GTAAAGACAA CACCTTTGAA GCCCTCATCT 
CTGCCTGTCA TTCCTGATAC TATTAAGGAA GTTATCTATG ATATGCTGAA TGCTTTGGCT 
GCATACCATG CTCCAGAGGA AGCAGACAAA TCTGATCCTA AACCTGGGGG AACGACCCAA 
GAGGTTGGCC AGCTCCTGCA AGACATGGGT GACGATGTAT ACCAGCAGTA CCGGTCACTT 
ACTCGTCAGA GCAGTGACTT TGATACACAG TCAGGTTTTT CCTTAAATAG TCAGGTTTTT 
GCTGCAGATG GTGCCCCTGC TGAAACTTCC ACAACTGGAA CCTCCCAAGG AGAGGGAGCT 
TCAACTCCAG AGGAGACTCG AGAAGGGAAG AAAGATAAAG AAGGGGACCG TACATCTGAG 
GAAGGCAAGC AGAAAAGCAA GGGCAGCAAA CCTTTAATGC CTACCTCTAC TATCCTTCGT 
CTTCTGGCAG AGTTGGTGAG GTCCTATGTT GGTATTGCTA CCCTGATTGC AAACTACAGC 
TATACCGTGG GCCAGTCTGA GCTGATCAAA GAGGACTGCA GTGTGCTAGC TTTTGTCCTG 
GACCACCTCC TCCCACACAC CCAGAATGCA GAAGACAAGG ATACACCTGC CCTGGCACGT 
CTATTCCTTG CAAGTCTGGC TGCTGCGGGG AGTGGCACAG ATGCTCAAGT GGCCCTAGTG 
AATGAAGTGA AAGCAGCCCT GGGACGGGCG CTTGCTATGG CTGAGAGTAC AGAGAAGCAC 
GCCAGACTTC AGGCAGTGAT GTGTATCATC AGTACTATCA TGGAATCCTG CCCCTCCACC 
TCCAGCTTCT ACAGTAGTGC TACAGCCAAG ACTCAGCATA ATGGCATGAA CAACATCATT 
CGACTGTTCC TAAAGAAGGG ACTAGTCAAT GACCTGGCCA GAGTTCCTCA CAGCCTAGAC 
CTGTCCAGTC CCAATATGGC CAACACAGTC AATGCTGCTC TAAAACCCTT GGAAACACTT 
TCCCGGATTG TAAATCAACC CAGTAGCCTT TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC 
AAGTCTGAGC AGGATGCTCA AGGAGCTTCT CAGGACTCCA GCAGCCATCA GCAAGATCCA 
GGCGAGCCTG GGGAGGCAGA AGTGCAGGAG GAGGATCATG ATGTCACTCA GACAGAGGTG 
GCAGATGGGG ATATCATGGA TGGGGAGGCT GAAACCGACT CAGTGGTGAT TGCCGGGCAG 
CCTGAGGTGC TCAGTTCACA AGAGATGCAG GTTGAAAATG AACTGGAGGA CCTGATAGAT 
GAGTTGCTTG AGAGGGATGG CGGATCTGGG AACAGTACAA TTATAGTGAG CAGAAGTGGA 
GAGGATGAAT CACAAGAGGA CGTGCTGATG GATGAAGCTC CTTCCAACCT CAGCCAAGCT 
TCCACCTTGC AGGCCAACCG AGAAGATTCC ATGAATATCC TGGACCCTGA GGATGAGGAG 
GAACATACTC AGGAAGAGGA CAGCAGTGGC AGTAACGAGG ATGAGGATGA TAGTCAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGAGGAA GATGATCAGG AGGATGATGA AGGTGAAGAA 
GGAGATGAAG ATGATGACGA CGATGGTTCT GAGATGGAAT TGGATGAGGA TTACCCTGAT 
ATGAACGCTT CTCCCTTGGT CCGATTTGAG CGCTTTGACC GGGAGGATGA TCTCATCATT 
GAGTTTGACA ACATGTTCTC CAGTGCTACA GACATTCCCC CATCCCCAGG AAATATCCCT 
ACCACCCACC CATTGATGGT ACGTCATGCA GATCACAGTT CCCTGACACT GGGCAGTGGT 
TCTTCCACAA CTCGTCTTAC CCAGGGCATC GGTCGCAGTC AAAGGACCCT CAGACAGCTC 
ACTGCCAATA CAGGCCATAC CATTCATGTT CACTACCCTG GGAATCGCCA GCCTAACCCT 
CCTCTTATAC TCCAGAGGTT GCTTGGTCCC TCTGCAGCTG CTGACATTCT TCAGCTGAGC 
AGCAGCCTTC CATTACAAAG CCGAGGCCGG GCCCGCCTCC TAGTGGGCAA TGATGACGTC 
CATATCATCG CCAGGTCTGA TGACGAGCTG CTGGATGACT TTTTTCATGA CCAGAGTACA 
GCCACTAGCC AAGCAGGAAC CCTGTCCAGC ATCCCAACAG CTCTAACCCG ATGGACAGAG 
GAATGCAAAG TTCTTGATGC TGAGAGTATG CATGACTGTG TTTCAGTGGT TAAAGTGCCC 
ATTGTCAATC ACCTGGAATT CTTGAGAGAT GAGGAGCTAG AAGAGAGAAG GGAGAAACGC 
AGGAAACAAT TGGCTGAGGA AGAAACAAAA ATAATAGACA AAGGCAAAGA AGACAAGGAG 
AACAGAGATC AGAGTGCACA GTGTACTGTA ACTAAGACAA ATGACTCCAC TGAACAGAAT 
GTCTCAGATG GGACTCCTAT GCCTGACAGC TATCCAACAA CCCCCTCTTC AACGGATGCA 
CCTACATCTG AGTCCAAGGA AACACTTGGT ACCCTACAAC CCTCCCAGCA ACAGCCAGCA 
CTTCCACCCC CACCATCCTT AGGAGAGATT CCTCAGGAAC TACAGTCCCC TGCTGAAGAA 
GTGGCAAACT CTACACAGCT ATTGATGCCT ATAGAGTTGG AGGAATTGGG TCCCACAAGA 
CCAAGTGGAG AAGCAGAAAC AACTCAAATG GAATTGTCTC CAGCTCCCAC CATAACCTCT 
CTTTCCCCAG AGAGAGCTGA AGATTCAGAC GCGTTAACAG CTGTCAGCAG TCAGCTGGAA 
GGTTCTCCCA TGGACACCAG CAGCCTGGCT TCCTGTACGT TAGAAGAGGC TGTGGGTGAC 
ACTCCAGCAG CTGGCAGTTC TGAGCAGCCC ACAGCAGGTA GTTCCACTCC TGGGGATGCG 
CCATCAGTTG TGGCAGAAGT GCAAGGCAGA CCTGATGTAT CAAGAGAATC CAACCAGCCA 
CCTGAGGACA GCTCCCCCCC TGCATCCTCG GAGAGTTCTT CCACTAGAGA TTCTGCCGTG 
GCCATCTCTG GAGCAGATTC TCGTGGAATT CTAGAAGAGC CACTACCTTC AACAAGCAGT 
GAAGAAGAAG ATCCCCTTGC TGGTATCAGT CTCCCTGAAG GTGTGGATCC CTCCTTTCTG 
GCTGCTCTCC CTGATGATAT CCGTCGGGAA GTCCTACAGA ACCAGCTGGG CATACGTCCA 
CCAACCCGAA GTGCTCCCTC CTCAAATAGC TCAGCTCCTG CAGTAGTGGG GAACCCTGGT 
GTAACTGAAG TGAGCCCTGA GTTCCTGGCT GCCCTACCTC CAGCTATTCA AGAGGAAGTG 
CTAGCACAGC AGAGAGCTGA ACAGCAGCGA CGGGAACTGG CTCAGAATGC GAGCTCAGAC 
ACCCCCATGG ACCCTGTGAC TTTCATTCAG ACTTTGCCCT CAGATCTGCG CCGTAGTGTC 
CTAGAGGATA TGGAAGACAG TGTGTTAGCT GTGATGCCAC CTGACATTGC AGCTGAGGCT 
CAAGCTCTAA GACGAGAACA AGAAGCCCGA CAGCGGCAGC TCATGCATGA GCGTCTTTTT 
GGACACAGTA GTACTTCTGC ACTCTCTGCC ATTCTCCGAA GCCCGGCTTT TACCAGTCGC 
CTGAGTGGCA ACCGTGGGGT CCAGTATACT CGCCTTGCTG TACAGAGGGG TGGCACCTTC 
CAGATGGGGG GTAGCAGCAG CCATAATAGG CCTTCTGGCA GTAATGTGGA CACACTACTC 
CGCCTCCGAG GACGACTCCT TCTAGACCAT GAAGCCCTGT CTTGCCTCTT GGTCCTACTT 
TTTGTGGATG AGCCAAAGCT CAATACTAGC CGTCTACACC GAGTACTGAG AAATCTCTGC 
TATCATGCCC AGACCCGCCA CTGGGTCATC CGCAGCCTCC TGTCCATTTT GCAGCGCAGC 
AGTGAAAGTG AGTTATGCAT TGAGACACCT AAGCTCTCCA CAAGTGAAGA AAGGGGCAAG 
AAATCAAGCA AGAGCTGTGC ATCAAGCAGT CATGAGAACC GTCCCCTGGA TCTGCTCCAC 
AAGATGGAGT CCAAAAGTTC CAATCAGCTC TCCTGGCTCT CAGTATCAAT GGATGCTGCT 
TTAGGCTGTA GGACTAACAT ATTCCAGATT CAACGTTCTG GAGGGCGTAA ACATACAGAG 
AAGCATGCAA GCAGTGGTTC CACTGTCCAC ATCCACCCCC AGGCTGCTCC TGTTGTTTGC 
AGACATGTTT TGGATACACT CATTCAGTTG GCCAAGGTGT TTCCCAGCCA CTTTACACAG 
CAGCGGACCA AAGAAACAAA CTGTGAGAGT GATCGGGAAC GGGGCAGTAA GCAGGCCTGC 
AGCCCATGCT CCTCACAGTC CTCCAGCAGT GGCATCTGTA CAGACTTCTG GGACTTATTG 
GTAAAACTGG ACAACATGAA TGTTAGCCGA AAAGGCAAGA ACTCCGTGAA GTCAGTACCA 
GTGAGCTCTG GTGGTGAAGG GGAAACTTCC CCACACAGCC TTGAAGCCTC TCCACTGGGG 
CAGCTCATGA ACATGTTGTC ACATCCAGTC ATCCGCCGGA GTTCTCTCTT AACTGAGAAA 
CTCCTGAGAC TCCTCTCTCT CATCTCAATT GCTCTCCCAG AAAACAAAGT ATCAGAAGTG 
CAAACTAACT CCAGCAACAG TGGTTCCTCC ACTGCTGCCA CCTCAAACAC ATCTACTACT 
ACCACCACCA CCACTACTGC CACTGCCCCC ACGCCCACAC CCCCAGCTGC AACTACCCCT 
GTCACTTCTG CTCCAGCTCT GGTTGCTGCC ACAGCTATTT CCACCATTAC TGTAGCTGCT 
TCTACCACAG TGACTACCCC CACAACTGCT ACCACTACTG TTTCAACTTC TACTACTAAA 
GGCAGCAAAT CTCCAGCAAA GGTTGGTGAA GGAGGCAGTG GTATAGACTT CAAAATGGTG 
TCCTCTGGCC TCACTGAAAA CCAGCTACAG CTCTCTGTAG AGGTGTTGAC ATCCCACTCT 
TGTTCTGAAG AAGGCTTGGA AGATGCGGCC AATGTGTTAC TGCAGCTCTC TCGTGGGGAC 
TCTGGTACCC GGGACACTGT TCTTAAGCTG CTACTAAATG GAGCCCGCCA TCTGGGTTAT 
ACCCTCTGTA AACAGATAGG TACCCTACTC GCTGAACTAC GGGAATATAA TCTGGAGCAG 
CAGCGGCGAG CCCAATGCGA GACCCTCTCT CCTGATGGCC TACCTGAGGA GCAACCACAG 
ACCACCAAAC TGAAAGGCAA AATGCAGAGC AGGTTTGACA TGGCTGAAAA TGTGGTAATT 
GTGGCATCTC AGAAGCGACC CTTGGGTGGC CGGGAGCTCC AGCTGCCTTC TATGTCCATG 
TTGACATCCA AGACATCTAC CCAGAAGTTC TTCTTGAGGG TACTACAGGT CATCATCCAG 
CTGCGGGATG ACACACGACG AGCTAACAAG AAAGCCAAGC AGACAGGCAG GCTAGGTTCC 
TCCGGTTTAG GCTCAGCTAG CAGCATCCAG GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT 
GATGCCATTA TACAAATGGT ACGTGAGGGT CAAAGGGCGC GGAGACAGCA ACAAGCAGCA 
ACGTCGGAGT CTTCTAACCA GTCAGAGACA TCTGTCCGGA GAGAGGAATC GCCCATGGAT 
GTGGACCAGC CATCTCCTAG TGCTCAAGAT ACTCAGTCCA TTGTAATCTC AGATGGAACA 
CCACAGGGAG AGAAGGAGAA AGAGGAGAAA CCACCTGAGT TACCCCTGCT CAGTGAGCAA 
CTGAGCCTAG ATGAACTGTG GGACATGCTA GGGGAATGTT TAAAAGAACT GGAGGAATCT 
CATGACCAGC ATGCAGTATT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TTTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGATACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGATG AACCTCCTCC ACTCTCCCCC GCCCCCTTAA CCCCAGCCAC TCCATCCTCC 
CTTGACCCAT TCTTCTCCAG GGAGCCCTCA TCTATGCACA TTTCCTCGAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATACTA 
CGGCAGTCTA CCACCCATCT TGCTGATGGG CCTTTTGCTG TCTTGGTAGA TTATATCCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAA 
GGGCTCCGGA AAGAAGATAT GGCAGTGCAT GTCCGCCGTG ACCATGTATT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCTCCTGAA GAAATGAAGA ATCGATTGTA TATAGTGTTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGGGGG CTCCTGCGAG AGTGGTACAT GATCATCTCT 
CGAGAGATGT TCAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA CCGGGTCACC 
TACACCATCA ATCCATCTTC CCACTGTAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT 
GGACGAATTG TAGCCAAAGC TGTATATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG 
TCTTTCTACA AACACATCTT GGGCAAATCT GTCAGGTATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAGGGCCT AGTTTATCTG CTTGAAAATG ATGTCTCTAC ATTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTTCAAGAA TTTGGAGTAT GTGAAGTTCG TGACCTCAAA 
CCCAATGGAG CCAACATCTT GGTAACAGAG GAGAACAAGA AGGAATATGT ACACCTGGTT 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CAGCCTTCTT GGAGGGCTTC 
TATGAAATCA TTCCAAAGCG CCTCATATCC ATCTTTACTG AGCAGGAATT AGAGCTGCTC 
ATATCAGGGC TGCCTACCAT CGACATTGAT GATCTAAAAT CTAACACTGA GTACCACAAG 
TACCAATCCA ACTCTATTCA GATCCAGTGG TTTTGGAGAG CATTGCGTTC CTTTGACCAA 
GCAGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTACAAGGA 
TTTGCTGCCC TTGAAGGTAT GAATGGCATT CAGAAGTTTC AGATTCATCG AGATGACAGA 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AACTGGACCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAATG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529014.1
CDS	1265..14179
Translation	MSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528951.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528951.3

ORF Insert Sequence:

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGGAG TTCAGTGTAT TCCACAACGA GCAGCACTTC TCAAGTCCAT GTTGAATTTC 
CTCAAGAAGG CCATTCAAGA CCCTGCTTTC TCAGATGGCA TACGACATGT GATGGATGGT 
TCTTTACCTA CCTCTCTGAA ACACATCATC AGCAATGCAG AATACTATGG TCCATCACTC 
TTCCTCCTAG CTACTGAAGT GGTGACTGTG TTTGTATTTC AAGAGCCATC ACTGCTGTCC 
TCACTCCAGG ACAATGGACT GACAGATGTC ATGCTGCATG CACTGCTCAT CAAAGATGTT 
CCTGCTACAA GGGAAGTCCT CGGTTCCCTC CCAAATGTAT TCAGTGCACT TTGCTTGAAT 
GCCCGAGGTC TTCAGTCTTT TGTACAATGT CAGCCATTTG AACGTCTCTT CAAAGTTCTT 
CTGTCTCCTG ATTACCTCCC AGCCATGCGG AGGAGGAGGA GTTCTGATCC CCTTGGGGAT 
ACTGCATCCA ACCTGGGGAG TGCTGTTGAT GAGCTCATGA GACACCAGCC TACCCTCAAA 
ACAGATGCAA CAACTGCCAT CATCAAGTTA CTTGAAGAAA TCTGTAATCT TGGAAGAGAT 
CCTAAATACA TTTGTCAGAA GCCATCTATT CAGAAAGCGG ATGGTACTGC TACTGCTCCT 
CCTCCAAGGT CTAATCATGC TGCAGAAGAG GCTTCCAGTG AAGATGAGGA GGAAGAGGAA 
GTACAGGCCA TGCAGAGCTT TAATTCTGCC CAGCAGAATG AAACTGAGCC TAATCAGCAG 
GTCGTTGGTA CCGAGGAACG TATACCTATA CCCCTCATGG ATTACATCCT TAATGTGATG 
AAATTTGTGG AATCTATTTT GAGCAACAAT ACAACAGATG ACCACTGTCA AGAATTTGTA 
AACCAGAAAG GACTCTTACC TTTGGTTACC ATTTTGGGTC TTCCCAATCT GCCCATTGAC 
TTTCCCACAT CTGCTGCCTG TCAGGCTGTT GCAGGTGTCT GCAAATCCAT ATTGACCCTG 
TCACATGAAC CCAAAGTTCT TCAAGAAGGT CTCCTTCAAT TAGACTTGAT CCTTTCCTCC 
TTGGAGCCTT TACATCGGCC GATTGAATCC CCTGGGGGCT CAGTGTTACT GAGAGAATTG 
GCATGTGCAG GCAATGTTGC TGATGCTACC CTCTCAGCTC AGGCCACGCC TCTGTTGCAT 
GCACTCACTG CAGCCCATGC TTACATCATG ATGTTTGTTC ACACTTGCAG AGTTGGACAG 
AGTGAAATTC GTTCTATCTC AGTAAACCAG TGGGGTTCAC AATTGGGTTT GAGTGTTCTG 
AGCAAGCTAA GCCAGTTATA CTGTTCTCTG GTGTGGGAAA GCACTGTCCT CCTCTCTCTA 
TGTACCCCCA ACAGCCTACC ATCTGGATGT GAATTTGGTC AAGCGGACAT GCAGAAACTA 
GTTCCAAAGG ATGAGAAGGC AGGTACGACC CAGGGCGGAA AAAGATCAGA TGGAGAACAG 
GATGGAACTG CTGGAAGCAT GGATGCATCT GCCCAGGGTT TATTAGAAGG AATTGAACTA 
GATGGTGACA CACTGGCACC CATGGAGACA GATGAACCTT CTTCTTCAGA TTCTAAGGGC 
AAATCTAAAA TCACACCTGC CATGGCTGCA AGAATTAAAC AAATCAAGCC GTTGCTGTCA 
GCTTCTTCCA GATTAGGCCG AGCACTTGCT GAGCTCTTTG GACTCCTTGT TAAGCTTTGT 
GTGGGTTCTC CTGTCCGCCA AAGGAGGAGC CATCATGCTG CCAGTACCAC CACTGCACCA 
ACACCTGCTG CTAGATCAAC AGCTTCAGCC CTCACTAAGC TACTCACAAA AGGCTTATCA 
TGGCAGCCCC CACCATATAC ACCCACTCCC CGCTTCAGGC TGACGTTCTT CATCTGTTCA 
GTTGGATTCA CCTCTCCAAT GCTCTTTGAT GAGAGAAAGT ATCCTTACCA CCTTATGCTG 
CAGAAATTTC TCTGCTCTGG TGGTCACAAT GCTCTTTTTG AAACTTTCAA CTGGGCTCTA 
TCCATGGGAG GAAAAGTTCC TGTTTCTGAG GGATTGGAAC ATTCAGATCT ACCTGATGGT 
ACAGGAGAAT TCCTAGATGC CTGGCTTATG CTTGTAGAAA AGATGGTAAA TCCCACCACG 
GTGCTTGAAT CTCCACATTC GTTGCCTGCT AAATTGCCTG GAGGAGTCCA GAGCTTTCCA 
CAATTTAGTG CCCTTCGCTT CCTTGTAGTA ACTCAAAAAG CAGCCTTTAC TTGTATCAAG 
AACCTGTGGA ACCGGAAACC CCTTAAAGTA TATGGGGGAA GAATGGCTGA GTCTATGCTG 
GCCATTTTGT GCCACATTCT CCGAGGGGAA CCTGTGATTC GAGAAAGACT GAGCAAAGAG 
AAGGAAGGAT CTCGAGGAGA AGAAGAAGCA GGCCAGGAAG AAGGTGGCTC ACGCAGAGAA 
CCTCAAGTCA ACCAGCAACA GCTACAACAG CTCATGGACA TGGGTTTCAC AAGAGAACAT 
GCCATGGAGG CACTGTTGAA TACCAGTACG ATGGAACAAG CAACAGAGTA CCTTTTAACC 
CACCCTCCTC CAATCATAGG AGGAGTTGTT CGGGATCTTA GCATGTCAGA AGAGGACCAG 
ATGATGAGAG CAATTGCTAT GTCTCTGGGA CAGGATATTC CAATGGATCA AAGGGCAGAG 
TCACCTGAGG AAGTTGCTTG CCGGAAGGAG GAAGAAGAGC GTAAAGCTCG AGAAAAACAA 
GAAGAGGAAG AAGCTAAATG TCTAGAGAAG TTTCAAGATG CTGACCCCTT GGAACAAGAT 
GAGCTCCACA CTTTCACAGA TACAATGTTA CCAGGCTGCT TCCATCTTCT TGATGAGTTG 
CCTGATACAG TGTACCGTGT TTGTGATCTG ATCATGACAG CTATCAAACG TAATGGAGCT 
GACTATCGTG ATATGATTCT AAAGCAAGTT GTCAACCAGG TGTGGGAAGC TGCTGATGTA 
TTGATCAAAG CTGCTCTTCC TCTAACAACA AGTGACACAA AAACAGTTTC AGAGTGGATC 
AGTCAGATGG CTACCCTGCC ACAGGCCTCC AATTTGGCTA CTAGAATCTT GCTTTTAACA 
CTACTTTTTG AGGAGTTGAA GTTACCTTGT GCCTGGGTGG TTGAATCAAG TGGCATTCTT 
AATGTCCTAA TCAAACTCTT AGAAGTGGTT CAACCCTGCC TACAGGCTGC CAAAGAGCAA 
AAGGAAGTCC AGACCCCAAA GTGGATCACC CCAGTGTTGC TCCTTATTGA TTTTTATGAA 
AAGACAGCCA TCTCATCAAA GAGAAGAGCC CAAATGACTA AGTACCTACA GTCCAACAGC 
AACAACTGGC GCTGGTTTGA TGACCGCTCT GGGCGTTGGT GTAGTTACAG TGCTAGCAAC 
AACAGCACCA TTGATTCTGC CTGGAAATCT GGAGAAACAA GTGTAAGATT CACTGCTGGC 
CGACGAAGAT ACACTGTCCA GTTCACTACA ATGGTGCAGG TTAATGAAGA AACAGGAAAC 
CGACGCCCTG TGATGCTGAC TCTCCTCAGG GTGCCACGAC TGAGTAAGAA CTCAAAAAGC 
AGCAATGGAC AGGAACTAGA GAAGACACTG GAAGAAAGCA AGGAGACCGA TATCAAACAT 
AAAGAAAATA AAGGCAATGA TATCCCCTTA GCTCTAGAGA GCACAAACAC TGAAAAAGAG 
GCAAGCCTGG ATGAAACAAA AATTGGGGAG ATCCTAATCC AGGGCCTGAC AGAAGATATG 
GTGACTGTTC TAATCCGTGC CTGTGTGAGC ATGCTGGGAG TCCCTGTGGA CCCAGACACT 
TTGCATGCCA CCCTTCGTCT CTGTTTAAGG CTCACCCGAG ACCACAAATA TGCCATGATG 
TTTGCAGAAC TGAAGAGTAC ACGAATGATC TTGAATTTGA CCCAGAGCTC AGGTTTCAAT 
GGGTTTACCC CCTTAGTCAC CCTTCTCTTA AGGCACATCA TTGAGGATCC TTGTACTCTT 
CGTCATACTA TGGAAAAGGT TGTTCGCTCG GCAGCCACAA GTGGAGCTGG TAGTACTACA 
TCTGGTGTTG TATCTGGCAG CCTCGGTTCT CGGGAGATCA ACTATATCCT TAGAGTCCTA 
GGACCAGCTG CCTGCCGCAA TCCAGACATA TTCACAGAAG TAGCCAATTG CTGTATCCGC 
ATTGCCCTTC CAGCCCCTCG AGGCTCAGGA ACTGCTTCAG ATGATGAATT TGAGAATCTT 
AGAATTAAAG GTCCTAATGC TGTACAGCTG GTAAAGACAA CACCTTTGAA GCCCTCATCT 
CTGCCTGTCA TTCCTGATAC TATTAAGGAA GTTATCTATG ATATGCTGAA TGCTTTGGCT 
GCATACCATG CTCCAGAGGA AGCAGACAAA TCTGATCCTA AACCTGGGGG AACGACCCAA 
GAGGTTGGCC AGCTCCTGCA AGACATGGGT GACGATGTAT ACCAGCAGTA CCGGTCACTT 
ACTCGTCAGA GCAGTGACTT TGATACACAG TCAGGTTTTT CCTTAAATAG TCAGGTTTTT 
GCTGCAGATG GTGCCCCTGC TGAAACTTCC ACAACTGGAA CCTCCCAAGG AGAGGGAGCT 
TCAACTCCAG AGGAGACTCG AGAAGGGAAG AAAGATAAAG AAGGGGACCG TACATCTGAG 
GAAGGCAAGC AGAAAAGCAA GGGCAGCAAA CCTTTAATGC CTACCTCTAC TATCCTTCGT 
CTTCTGGCAG AGTTGGTGAG GTCCTATGTT GGTATTGCTA CCCTGATTGC AAACTACAGC 
TATACCGTGG GCCAGTCTGA GCTGATCAAA GAGGACTGCA GTGTGCTAGC TTTTGTCCTG 
GACCACCTCC TCCCACACAC CCAGAATGCA GAAGACAAGG ATACACCTGC CCTGGCACGT 
CTATTCCTTG CAAGTCTGGC TGCTGCGGGG AGTGGCACAG ATGCTCAAGT GGCCCTAGTG 
AATGAAGTGA AAGCAGCCCT GGGACGGGCG CTTGCTATGG CTGAGAGTAC AGAGAAGCAC 
GCCAGACTTC AGGCAGTGAT GTGTATCATC AGTACTATCA TGGAATCCTG CCCCTCCACC 
TCCAGCTTCT ACAGTAGTGC TACAGCCAAG ACTCAGCATA ATGGCATGAA CAACATCATT 
CGACTGTTCC TAAAGAAGGG ACTAGTCAAT GACCTGGCCA GAGTTCCTCA CAGCCTAGAC 
CTGTCCAGTC CCAATATGGC CAACACAGTC AATGCTGCTC TAAAACCCTT GGAAACACTT 
TCCCGGATTG TAAATCAACC CAGTAGCCTT TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC 
AAGTCTGAGC AGGATGCTCA AGGAGCTTCT CAGGACTCCA GCAGCCATCA GCAAGATCCA 
GGCGAGCCTG GGGAGGCAGA AGTGCAGGAG GAGGATCATG ATGTCACTCA GACAGAGGTG 
GCAGATGGGG ATATCATGGA TGGGGAGGCT GAAACCGACT CAGTGGTGAT TGCCGGGCAG 
CCTGAGGTGC TCAGTTCACA AGAGATGCAG GTTGAAAATG AACTGGAGGA CCTGATAGAT 
GAGTTGCTTG AGAGGGATGG CGGATCTGGG AACAGTACAA TTATAGTGAG CAGAAGTGGA 
GAGGATGAAT CACAAGAGGA CGTGCTGATG GATGAAGCTC CTTCCAACCT CAGCCAAGCT 
TCCACCTTGC AGGCCAACCG AGAAGATTCC ATGAATATCC TGGACCCTGA GGATGAGGAG 
GAACATACTC AGGAAGAGGA CAGCAGTGGC AGTAACGAGG ATGAGGATGA TAGTCAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGAGGAA GATGATCAGG AGGATGATGA AGGTGAAGAA 
GGAGATGAAG ATGATGACGA CGATGGTTCT GAGATGGAAT TGGATGAGGA TTACCCTGAT 
ATGAACGCTT CTCCCTTGGT CCGATTTGAG CGCTTTGACC GGGAGGATGA TCTCATCATT 
GAGTTTGACA ACATGTTCTC CAGTGCTACA GACATTCCCC CATCCCCAGG AAATATCCCT 
ACCACCCACC CATTGATGGT ACGTCATGCA GATCACAGTT CCCTGACACT GGGCAGTGGT 
TCTTCCACAA CTCGTCTTAC CCAGGGCATC GGTCGCAGTC AAAGGACCCT CAGACAGCTC 
ACTGCCAATA CAGGCCATAC CATTCATGTT CACTACCCTG GGAATCGCCA GCCTAACCCT 
CCTCTTATAC TCCAGAGGTT GCTTGGTCCC TCTGCAGCTG CTGACATTCT TCAGCTGAGC 
AGCAGCCTTC CATTACAAAG CCGAGGCCGG GCCCGCCTCC TAGTGGGCAA TGATGACGTC 
CATATCATCG CCAGGTCTGA TGACGAGCTG CTGGATGACT TTTTTCATGA CCAGAGTACA 
GCCACTAGCC AAGCAGGAAC CCTGTCCAGC ATCCCAACAG CTCTAACCCG ATGGACAGAG 
GAATGCAAAG TTCTTGATGC TGAGAGTATG CATGACTGTG TTTCAGTGGT TAAAGTGCCC 
ATTGTCAATC ACCTGGAATT CTTGAGAGAT GAGGAGCTAG AAGAGAGAAG GGAGAAACGC 
AGGAAACAAT TGGCTGAGGA AGAAACAAAA ATAATAGACA AAGGCAAAGA AGACAAGGAG 
AACAGAGATC AGAGTGCACA GTGTACTGTA ACTAAGACAA ATGACTCCAC TGAACAGAAT 
GTCTCAGATG GGACTCCTAT GCCTGACAGC TATCCAACAA CCCCCTCTTC AACGGATGCA 
CCTACATCTG AGTCCAAGGA AACACTTGGT ACCCTACAAC CCTCCCAGCA ACAGCCAGCA 
CTTCCACCCC CACCATCCTT AGGAGAGATT CCTCAGGAAC TACAGTCCCC TGCTGAAGAA 
GTGGCAAACT CTACACAGCT ATTGATGCCT ATAGAGTTGG AGGAATTGGG TCCCACAAGA 
CCAAGTGGAG AAGCAGAAAC AACTCAAATG GAATTGTCTC CAGCTCCCAC CATAACCTCT 
CTTTCCCCAG AGAGAGCTGA AGATTCAGAC GCGTTAACAG CTGTCAGCAG TCAGCTGGAA 
GGTTCTCCCA TGGACACCAG CAGCCTGGCT TCCTGTACGT TAGAAGAGGC TGTGGGTGAC 
ACTCCAGCAG CTGGCAGTTC TGAGCAGCCC ACAGCAGGTA GTTCCACTCC TGGGGATGCG 
CCATCAGTTG TGGCAGAAGT GCAAGGCAGA CCTGATGTAT CAAGAGAATC CAACCAGCCA 
CCTGAGGACA GCTCCCCCCC TGCATCCTCG GAGAGTTCTT CCACTAGAGA TTCTGCCGTG 
GCCATCTCTG GAGCAGATTC TCGTGGAATT CTAGAAGAGC CACTACCTTC AACAAGCAGT 
GAAGAAGAAG ATCCCCTTGC TGGTATCAGT CTCCCTGAAG GTGTGGATCC CTCCTTTCTG 
GCTGCTCTCC CTGATGATAT CCGTCGGGAA GTCCTACAGA ACCAGCTGGG CATACGTCCA 
CCAACCCGAA GTGCTCCCTC CTCAAATAGC TCAGCTCCTG CAGTAGTGGG GAACCCTGGT 
GTAACTGAAG TGAGCCCTGA GTTCCTGGCT GCCCTACCTC CAGCTATTCA AGAGGAAGTG 
CTAGCACAGC AGAGAGCTGA ACAGCAGCGA CGGGAACTGG CTCAGAATGC GAGCTCAGAC 
ACCCCCATGG ACCCTGTGAC TTTCATTCAG ACTTTGCCCT CAGATCTGCG CCGTAGTGTC 
CTAGAGGATA TGGAAGACAG TGTGTTAGCT GTGATGCCAC CTGACATTGC AGCTGAGGCT 
CAAGCTCTAA GACGAGAACA AGAAGCCCGA CAGCGGCAGC TCATGCATGA GCGTCTTTTT 
GGACACAGTA GTACTTCTGC ACTCTCTGCC ATTCTCCGAA GCCCGGCTTT TACCAGTCGC 
CTGAGTGGCA ACCGTGGGGT CCAGTATACT CGCCTTGCTG TACAGAGGGG TGGCACCTTC 
CAGATGGGGG GTAGCAGCAG CCATAATAGG CCTTCTGGCA GTAATGTGGA CACACTACTC 
CGCCTCCGAG GACGACTCCT TCTAGACCAT GAAGCCCTGT CTTGCCTCTT GGTCCTACTT 
TTTGTGGATG AGCCAAAGCT CAATACTAGC CGTCTACACC GAGTACTGAG AAATCTCTGC 
TATCATGCCC AGACCCGCCA CTGGGTCATC CGCAGCCTCC TGTCCATTTT GCAGCGCAGC 
AGTGAAAGTG AGTTATGCAT TGAGACACCT AAGCTCTCCA CAAGTGAAGA AAGGGGCAAG 
AAATCAAGCA AGAGCTGTGC ATCAAGCAGT CATGAGAACC GTCCCCTGGA TCTGCTCCAC 
AAGATGGAGT CCAAAAGTTC CAATCAGCTC TCCTGGCTCT CAGTATCAAT GGATGCTGCT 
TTAGGCTGTA GGACTAACAT ATTCCAGATT CAACGTTCTG GAGGGCGTAA ACATACAGAG 
AAGCATGCAA GCAGTGGTTC CACTGTCCAC ATCCACCCCC AGGCTGCTCC TGTTGTTTGC 
AGACATGTTT TGGATACACT CATTCAGTTG GCCAAGGTGT TTCCCAGCCA CTTTACACAG 
CAGCGGACCA AAGAAACAAA CTGTGAGAGT GATCGGGAAC GGGGCAGTAA GCAGGCCTGC 
AGCCCATGCT CCTCACAGTC CTCCAGCAGT GGCATCTGTA CAGACTTCTG GGACTTATTG 
GTAAAACTGG ACAACATGAA TGTTAGCCGA AAAGGCAAGA ACTCCGTGAA GTCAGTACCA 
GTGAGCTCTG GTGGTGAAGG GGAAACTTCC CCACACAGCC TTGAAGCCTC TCCACTGGGG 
CAGCTCATGA ACATGTTGTC ACATCCAGTC ATCCGCCGGA GTTCTCTCTT AACTGAGAAA 
CTCCTGAGAC TCCTCTCTCT CATCTCAATT GCTCTCCCAG AAAACAAAGT ATCAGAAGTG 
CAAACTAACT CCAGCAACAG TGGTTCCTCC ACTGCTGCCA CCTCAAACAC ATCTACTACT 
ACCACCACCA CCACTACTGC CACTGCCCCC ACGCCCACAC CCCCAGCTGC AACTACCCCT 
GTCACTTCTG CTCCAGCTCT GGTTGCTGCC ACAGCTATTT CCACCATTAC TGTAGCTGCT 
TCTACCACAG TGACTACCCC CACAACTGCT ACCACTACTG TTTCAACTTC TACTACTAAA 
GGCAGCAAAT CTCCAGCAAA GGTTGGTGAA GGAGGCAGTG GTATAGACTT CAAAATGGTG 
TCCTCTGGCC TCACTGAAAA CCAGCTACAG CTCTCTGTAG AGGTGTTGAC ATCCCACTCT 
TGTTCTGAAG AAGGCTTGGA AGATGCGGCC AATGTGTTAC TGCAGCTCTC TCGTGGGGAC 
TCTGGTACCC GGGACACTGT TCTTAAGCTG CTACTAAATG GAGCCCGCCA TCTGGGTTAT 
ACCCTCTGTA AACAGATAGG TACCCTACTC GCTGAACTAC GGGAATATAA TCTGGAGCAG 
CAGCGGCGAG CCCAATGCGA GACCCTCTCT CCTGATGGCC TACCTGAGGA GCAACCACAG 
ACCACCAAAC TGAAAGGCAA AATGCAGAGC AGGTTTGACA TGGCTGAAAA TGTGGTAATT 
GTGGCATCTC AGAAGCGACC CTTGGGTGGC CGGGAGCTCC AGCTGCCTTC TATGTCCATG 
TTGACATCCA AGACATCTAC CCAGAAGTTC TTCTTGAGGG TACTACAGGT CATCATCCAG 
CTGCGGGATG ACACACGACG AGCTAACAAG AAAGCCAAGC AGACAGGCAG GCTAGGTTCC 
TCCGGTTTAG GCTCAGCTAG CAGCATCCAG GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT 
GATGCCATTA TACAAATGGT ACGTGAGGGT CAAAGGGCGC GGAGACAGCA ACAAGCAGCA 
ACGTCGGAGT CTTCTAACCA GTCAGAGACA TCTGTCCGGA GAGAGGAATC GCCCATGGAT 
GTGGACCAGC CATCTCCTAG TGCTCAAGAT ACTCAGTCCA TTGTAATCTC AGATGGAACA 
CCACAGGGAG AGAAGGAGAA AGAGGAGAAA CCACCTGAGT TACCCCTGCT CAGTGAGCAA 
CTGAGCCTAG ATGAACTGTG GGACATGCTA GGGGAATGTT TAAAAGAACT GGAGGAATCT 
CATGACCAGC ATGCAGTATT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TTTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGATACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGATG AACCTCCTCC ACTCTCCCCC GCCCCCTTAA CCCCAGCCAC TCCATCCTCC 
CTTGACCCAT TCTTCTCCAG GGAGCCCTCA TCTATGCACA TTTCCTCGAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATACTA 
CGGCAGTCTA CCACCCATCT TGCTGATGGG CCTTTTGCTG TCTTGGTAGA TTATATCCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAA 
GGGCTCCGGA AAGAAGATAT GGCAGTGCAT GTCCGCCGTG ACCATGTATT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCTCCTGAA GAAATGAAGA ATCGATTGTA TATAGTGTTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGGGGG CTCCTGCGAG AGTGGTACAT GATCATCTCT 
CGAGAGATGT TCAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA CCGGGTCACC 
TACACCATCA ATCCATCTTC CCACTGTAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT 
GGACGAATTG TAGCCAAAGC TGTATATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG 
TCTTTCTACA AACACATCTT GGGCAAATCT GTCAGGTATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAGGGCCT AGTTTATCTG CTTGAAAATG ATGTCTCTAC ATTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTTCAAGAA TTTGGAGTAT GTGAAGTTCG TGACCTCAAA 
CCCAATGGAG CCAACATCTT GGTAACAGAG GAGAACAAGA AGGAATATGT ACACCTGGTT 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CAGCCTTCTT GGAGGGCTTC 
TATGAAATCA TTCCAAAGCG CCTCATATCC ATCTTTACTG AGCAGGAATT AGAGCTGCTC 
ATATCAGGGC TGCCTACCAT CGACATTGAT GATCTAAAAT CTAACACTGA GTACCACAAG 
TACCAATCCA ACTCTATTCA GATCCAGTGG TTTTGGAGAG CATTGCGTTC CTTTGACCAA 
GCAGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTACAAGGA 
TTTGCTGCCC TTGAAGGTAT GAATGGCATT CAGAAGTTTC AGATTCATCG AGATGACAGA 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AACTGGACCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAATG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_006528951.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529014.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X22, mRNA.

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CloneID	OMu56721
Clone ID Related Accession (Same CDS sequence)	XM_006528951.3 , XM_006528951.2
Accession Version	XM_006528951.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006528951.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGGAG TTCAGTGTAT TCCACAACGA GCAGCACTTC TCAAGTCCAT GTTGAATTTC 
CTCAAGAAGG CCATTCAAGA CCCTGCTTTC TCAGATGGCA TACGACATGT GATGGATGGT 
TCTTTACCTA CCTCTCTGAA ACACATCATC AGCAATGCAG AATACTATGG TCCATCACTC 
TTCCTCCTAG CTACTGAAGT GGTGACTGTG TTTGTATTTC AAGAGCCATC ACTGCTGTCC 
TCACTCCAGG ACAATGGACT GACAGATGTC ATGCTGCATG CACTGCTCAT CAAAGATGTT 
CCTGCTACAA GGGAAGTCCT CGGTTCCCTC CCAAATGTAT TCAGTGCACT TTGCTTGAAT 
GCCCGAGGTC TTCAGTCTTT TGTACAATGT CAGCCATTTG AACGTCTCTT CAAAGTTCTT 
CTGTCTCCTG ATTACCTCCC AGCCATGCGG AGGAGGAGGA GTTCTGATCC CCTTGGGGAT 
ACTGCATCCA ACCTGGGGAG TGCTGTTGAT GAGCTCATGA GACACCAGCC TACCCTCAAA 
ACAGATGCAA CAACTGCCAT CATCAAGTTA CTTGAAGAAA TCTGTAATCT TGGAAGAGAT 
CCTAAATACA TTTGTCAGAA GCCATCTATT CAGAAAGCGG ATGGTACTGC TACTGCTCCT 
CCTCCAAGGT CTAATCATGC TGCAGAAGAG GCTTCCAGTG AAGATGAGGA GGAAGAGGAA 
GTACAGGCCA TGCAGAGCTT TAATTCTGCC CAGCAGAATG AAACTGAGCC TAATCAGCAG 
GTCGTTGGTA CCGAGGAACG TATACCTATA CCCCTCATGG ATTACATCCT TAATGTGATG 
AAATTTGTGG AATCTATTTT GAGCAACAAT ACAACAGATG ACCACTGTCA AGAATTTGTA 
AACCAGAAAG GACTCTTACC TTTGGTTACC ATTTTGGGTC TTCCCAATCT GCCCATTGAC 
TTTCCCACAT CTGCTGCCTG TCAGGCTGTT GCAGGTGTCT GCAAATCCAT ATTGACCCTG 
TCACATGAAC CCAAAGTTCT TCAAGAAGGT CTCCTTCAAT TAGACTTGAT CCTTTCCTCC 
TTGGAGCCTT TACATCGGCC GATTGAATCC CCTGGGGGCT CAGTGTTACT GAGAGAATTG 
GCATGTGCAG GCAATGTTGC TGATGCTACC CTCTCAGCTC AGGCCACGCC TCTGTTGCAT 
GCACTCACTG CAGCCCATGC TTACATCATG ATGTTTGTTC ACACTTGCAG AGTTGGACAG 
AGTGAAATTC GTTCTATCTC AGTAAACCAG TGGGGTTCAC AATTGGGTTT GAGTGTTCTG 
AGCAAGCTAA GCCAGTTATA CTGTTCTCTG GTGTGGGAAA GCACTGTCCT CCTCTCTCTA 
TGTACCCCCA ACAGCCTACC ATCTGGATGT GAATTTGGTC AAGCGGACAT GCAGAAACTA 
GTTCCAAAGG ATGAGAAGGC AGGTACGACC CAGGGCGGAA AAAGATCAGA TGGAGAACAG 
GATGGAACTG CTGGAAGCAT GGATGCATCT GCCCAGGGTT TATTAGAAGG AATTGAACTA 
GATGGTGACA CACTGGCACC CATGGAGACA GATGAACCTT CTTCTTCAGA TTCTAAGGGC 
AAATCTAAAA TCACACCTGC CATGGCTGCA AGAATTAAAC AAATCAAGCC GTTGCTGTCA 
GCTTCTTCCA GATTAGGCCG AGCACTTGCT GAGCTCTTTG GACTCCTTGT TAAGCTTTGT 
GTGGGTTCTC CTGTCCGCCA AAGGAGGAGC CATCATGCTG CCAGTACCAC CACTGCACCA 
ACACCTGCTG CTAGATCAAC AGCTTCAGCC CTCACTAAGC TACTCACAAA AGGCTTATCA 
TGGCAGCCCC CACCATATAC ACCCACTCCC CGCTTCAGGC TGACGTTCTT CATCTGTTCA 
GTTGGATTCA CCTCTCCAAT GCTCTTTGAT GAGAGAAAGT ATCCTTACCA CCTTATGCTG 
CAGAAATTTC TCTGCTCTGG TGGTCACAAT GCTCTTTTTG AAACTTTCAA CTGGGCTCTA 
TCCATGGGAG GAAAAGTTCC TGTTTCTGAG GGATTGGAAC ATTCAGATCT ACCTGATGGT 
ACAGGAGAAT TCCTAGATGC CTGGCTTATG CTTGTAGAAA AGATGGTAAA TCCCACCACG 
GTGCTTGAAT CTCCACATTC GTTGCCTGCT AAATTGCCTG GAGGAGTCCA GAGCTTTCCA 
CAATTTAGTG CCCTTCGCTT CCTTGTAGTA ACTCAAAAAG CAGCCTTTAC TTGTATCAAG 
AACCTGTGGA ACCGGAAACC CCTTAAAGTA TATGGGGGAA GAATGGCTGA GTCTATGCTG 
GCCATTTTGT GCCACATTCT CCGAGGGGAA CCTGTGATTC GAGAAAGACT GAGCAAAGAG 
AAGGAAGGAT CTCGAGGAGA AGAAGAAGCA GGCCAGGAAG AAGGTGGCTC ACGCAGAGAA 
CCTCAAGTCA ACCAGCAACA GCTACAACAG CTCATGGACA TGGGTTTCAC AAGAGAACAT 
GCCATGGAGG CACTGTTGAA TACCAGTACG ATGGAACAAG CAACAGAGTA CCTTTTAACC 
CACCCTCCTC CAATCATAGG AGGAGTTGTT CGGGATCTTA GCATGTCAGA AGAGGACCAG 
ATGATGAGAG CAATTGCTAT GTCTCTGGGA CAGGATATTC CAATGGATCA AAGGGCAGAG 
TCACCTGAGG AAGTTGCTTG CCGGAAGGAG GAAGAAGAGC GTAAAGCTCG AGAAAAACAA 
GAAGAGGAAG AAGCTAAATG TCTAGAGAAG TTTCAAGATG CTGACCCCTT GGAACAAGAT 
GAGCTCCACA CTTTCACAGA TACAATGTTA CCAGGCTGCT TCCATCTTCT TGATGAGTTG 
CCTGATACAG TGTACCGTGT TTGTGATCTG ATCATGACAG CTATCAAACG TAATGGAGCT 
GACTATCGTG ATATGATTCT AAAGCAAGTT GTCAACCAGG TGTGGGAAGC TGCTGATGTA 
TTGATCAAAG CTGCTCTTCC TCTAACAACA AGTGACACAA AAACAGTTTC AGAGTGGATC 
AGTCAGATGG CTACCCTGCC ACAGGCCTCC AATTTGGCTA CTAGAATCTT GCTTTTAACA 
CTACTTTTTG AGGAGTTGAA GTTACCTTGT GCCTGGGTGG TTGAATCAAG TGGCATTCTT 
AATGTCCTAA TCAAACTCTT AGAAGTGGTT CAACCCTGCC TACAGGCTGC CAAAGAGCAA 
AAGGAAGTCC AGACCCCAAA GTGGATCACC CCAGTGTTGC TCCTTATTGA TTTTTATGAA 
AAGACAGCCA TCTCATCAAA GAGAAGAGCC CAAATGACTA AGTACCTACA GTCCAACAGC 
AACAACTGGC GCTGGTTTGA TGACCGCTCT GGGCGTTGGT GTAGTTACAG TGCTAGCAAC 
AACAGCACCA TTGATTCTGC CTGGAAATCT GGAGAAACAA GTGTAAGATT CACTGCTGGC 
CGACGAAGAT ACACTGTCCA GTTCACTACA ATGGTGCAGG TTAATGAAGA AACAGGAAAC 
CGACGCCCTG TGATGCTGAC TCTCCTCAGG GTGCCACGAC TGAGTAAGAA CTCAAAAAGC 
AGCAATGGAC AGGAACTAGA GAAGACACTG GAAGAAAGCA AGGAGACCGA TATCAAACAT 
AAAGAAAATA AAGGCAATGA TATCCCCTTA GCTCTAGAGA GCACAAACAC TGAAAAAGAG 
GCAAGCCTGG ATGAAACAAA AATTGGGGAG ATCCTAATCC AGGGCCTGAC AGAAGATATG 
GTGACTGTTC TAATCCGTGC CTGTGTGAGC ATGCTGGGAG TCCCTGTGGA CCCAGACACT 
TTGCATGCCA CCCTTCGTCT CTGTTTAAGG CTCACCCGAG ACCACAAATA TGCCATGATG 
TTTGCAGAAC TGAAGAGTAC ACGAATGATC TTGAATTTGA CCCAGAGCTC AGGTTTCAAT 
GGGTTTACCC CCTTAGTCAC CCTTCTCTTA AGGCACATCA TTGAGGATCC TTGTACTCTT 
CGTCATACTA TGGAAAAGGT TGTTCGCTCG GCAGCCACAA GTGGAGCTGG TAGTACTACA 
TCTGGTGTTG TATCTGGCAG CCTCGGTTCT CGGGAGATCA ACTATATCCT TAGAGTCCTA 
GGACCAGCTG CCTGCCGCAA TCCAGACATA TTCACAGAAG TAGCCAATTG CTGTATCCGC 
ATTGCCCTTC CAGCCCCTCG AGGCTCAGGA ACTGCTTCAG ATGATGAATT TGAGAATCTT 
AGAATTAAAG GTCCTAATGC TGTACAGCTG GTAAAGACAA CACCTTTGAA GCCCTCATCT 
CTGCCTGTCA TTCCTGATAC TATTAAGGAA GTTATCTATG ATATGCTGAA TGCTTTGGCT 
GCATACCATG CTCCAGAGGA AGCAGACAAA TCTGATCCTA AACCTGGGGG AACGACCCAA 
GAGGTTGGCC AGCTCCTGCA AGACATGGGT GACGATGTAT ACCAGCAGTA CCGGTCACTT 
ACTCGTCAGA GCAGTGACTT TGATACACAG TCAGGTTTTT CCTTAAATAG TCAGGTTTTT 
GCTGCAGATG GTGCCCCTGC TGAAACTTCC ACAACTGGAA CCTCCCAAGG AGAGGGAGCT 
TCAACTCCAG AGGAGACTCG AGAAGGGAAG AAAGATAAAG AAGGGGACCG TACATCTGAG 
GAAGGCAAGC AGAAAAGCAA GGGCAGCAAA CCTTTAATGC CTACCTCTAC TATCCTTCGT 
CTTCTGGCAG AGTTGGTGAG GTCCTATGTT GGTATTGCTA CCCTGATTGC AAACTACAGC 
TATACCGTGG GCCAGTCTGA GCTGATCAAA GAGGACTGCA GTGTGCTAGC TTTTGTCCTG 
GACCACCTCC TCCCACACAC CCAGAATGCA GAAGACAAGG ATACACCTGC CCTGGCACGT 
CTATTCCTTG CAAGTCTGGC TGCTGCGGGG AGTGGCACAG ATGCTCAAGT GGCCCTAGTG 
AATGAAGTGA AAGCAGCCCT GGGACGGGCG CTTGCTATGG CTGAGAGTAC AGAGAAGCAC 
GCCAGACTTC AGGCAGTGAT GTGTATCATC AGTACTATCA TGGAATCCTG CCCCTCCACC 
TCCAGCTTCT ACAGTAGTGC TACAGCCAAG ACTCAGCATA ATGGCATGAA CAACATCATT 
CGACTGTTCC TAAAGAAGGG ACTAGTCAAT GACCTGGCCA GAGTTCCTCA CAGCCTAGAC 
CTGTCCAGTC CCAATATGGC CAACACAGTC AATGCTGCTC TAAAACCCTT GGAAACACTT 
TCCCGGATTG TAAATCAACC CAGTAGCCTT TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC 
AAGTCTGAGC AGGATGCTCA AGGAGCTTCT CAGGACTCCA GCAGCCATCA GCAAGATCCA 
GGCGAGCCTG GGGAGGCAGA AGTGCAGGAG GAGGATCATG ATGTCACTCA GACAGAGGTG 
GCAGATGGGG ATATCATGGA TGGGGAGGCT GAAACCGACT CAGTGGTGAT TGCCGGGCAG 
CCTGAGGTGC TCAGTTCACA AGAGATGCAG GTTGAAAATG AACTGGAGGA CCTGATAGAT 
GAGTTGCTTG AGAGGGATGG CGGATCTGGG AACAGTACAA TTATAGTGAG CAGAAGTGGA 
GAGGATGAAT CACAAGAGGA CGTGCTGATG GATGAAGCTC CTTCCAACCT CAGCCAAGCT 
TCCACCTTGC AGGCCAACCG AGAAGATTCC ATGAATATCC TGGACCCTGA GGATGAGGAG 
GAACATACTC AGGAAGAGGA CAGCAGTGGC AGTAACGAGG ATGAGGATGA TAGTCAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGAGGAA GATGATCAGG AGGATGATGA AGGTGAAGAA 
GGAGATGAAG ATGATGACGA CGATGGTTCT GAGATGGAAT TGGATGAGGA TTACCCTGAT 
ATGAACGCTT CTCCCTTGGT CCGATTTGAG CGCTTTGACC GGGAGGATGA TCTCATCATT 
GAGTTTGACA ACATGTTCTC CAGTGCTACA GACATTCCCC CATCCCCAGG AAATATCCCT 
ACCACCCACC CATTGATGGT ACGTCATGCA GATCACAGTT CCCTGACACT GGGCAGTGGT 
TCTTCCACAA CTCGTCTTAC CCAGGGCATC GGTCGCAGTC AAAGGACCCT CAGACAGCTC 
ACTGCCAATA CAGGCCATAC CATTCATGTT CACTACCCTG GGAATCGCCA GCCTAACCCT 
CCTCTTATAC TCCAGAGGTT GCTTGGTCCC TCTGCAGCTG CTGACATTCT TCAGCTGAGC 
AGCAGCCTTC CATTACAAAG CCGAGGCCGG GCCCGCCTCC TAGTGGGCAA TGATGACGTC 
CATATCATCG CCAGGTCTGA TGACGAGCTG CTGGATGACT TTTTTCATGA CCAGAGTACA 
GCCACTAGCC AAGCAGGAAC CCTGTCCAGC ATCCCAACAG CTCTAACCCG ATGGACAGAG 
GAATGCAAAG TTCTTGATGC TGAGAGTATG CATGACTGTG TTTCAGTGGT TAAAGTGCCC 
ATTGTCAATC ACCTGGAATT CTTGAGAGAT GAGGAGCTAG AAGAGAGAAG GGAGAAACGC 
AGGAAACAAT TGGCTGAGGA AGAAACAAAA ATAATAGACA AAGGCAAAGA AGACAAGGAG 
AACAGAGATC AGAGTGCACA GTGTACTGTA ACTAAGACAA ATGACTCCAC TGAACAGAAT 
GTCTCAGATG GGACTCCTAT GCCTGACAGC TATCCAACAA CCCCCTCTTC AACGGATGCA 
CCTACATCTG AGTCCAAGGA AACACTTGGT ACCCTACAAC CCTCCCAGCA ACAGCCAGCA 
CTTCCACCCC CACCATCCTT AGGAGAGATT CCTCAGGAAC TACAGTCCCC TGCTGAAGAA 
GTGGCAAACT CTACACAGCT ATTGATGCCT ATAGAGTTGG AGGAATTGGG TCCCACAAGA 
CCAAGTGGAG AAGCAGAAAC AACTCAAATG GAATTGTCTC CAGCTCCCAC CATAACCTCT 
CTTTCCCCAG AGAGAGCTGA AGATTCAGAC GCGTTAACAG CTGTCAGCAG TCAGCTGGAA 
GGTTCTCCCA TGGACACCAG CAGCCTGGCT TCCTGTACGT TAGAAGAGGC TGTGGGTGAC 
ACTCCAGCAG CTGGCAGTTC TGAGCAGCCC ACAGCAGGTA GTTCCACTCC TGGGGATGCG 
CCATCAGTTG TGGCAGAAGT GCAAGGCAGA CCTGATGTAT CAAGAGAATC CAACCAGCCA 
CCTGAGGACA GCTCCCCCCC TGCATCCTCG GAGAGTTCTT CCACTAGAGA TTCTGCCGTG 
GCCATCTCTG GAGCAGATTC TCGTGGAATT CTAGAAGAGC CACTACCTTC AACAAGCAGT 
GAAGAAGAAG ATCCCCTTGC TGGTATCAGT CTCCCTGAAG GTGTGGATCC CTCCTTTCTG 
GCTGCTCTCC CTGATGATAT CCGTCGGGAA GTCCTACAGA ACCAGCTGGG CATACGTCCA 
CCAACCCGAA GTGCTCCCTC CTCAAATAGC TCAGCTCCTG CAGTAGTGGG GAACCCTGGT 
GTAACTGAAG TGAGCCCTGA GTTCCTGGCT GCCCTACCTC CAGCTATTCA AGAGGAAGTG 
CTAGCACAGC AGAGAGCTGA ACAGCAGCGA CGGGAACTGG CTCAGAATGC GAGCTCAGAC 
ACCCCCATGG ACCCTGTGAC TTTCATTCAG ACTTTGCCCT CAGATCTGCG CCGTAGTGTC 
CTAGAGGATA TGGAAGACAG TGTGTTAGCT GTGATGCCAC CTGACATTGC AGCTGAGGCT 
CAAGCTCTAA GACGAGAACA AGAAGCCCGA CAGCGGCAGC TCATGCATGA GCGTCTTTTT 
GGACACAGTA GTACTTCTGC ACTCTCTGCC ATTCTCCGAA GCCCGGCTTT TACCAGTCGC 
CTGAGTGGCA ACCGTGGGGT CCAGTATACT CGCCTTGCTG TACAGAGGGG TGGCACCTTC 
CAGATGGGGG GTAGCAGCAG CCATAATAGG CCTTCTGGCA GTAATGTGGA CACACTACTC 
CGCCTCCGAG GACGACTCCT TCTAGACCAT GAAGCCCTGT CTTGCCTCTT GGTCCTACTT 
TTTGTGGATG AGCCAAAGCT CAATACTAGC CGTCTACACC GAGTACTGAG AAATCTCTGC 
TATCATGCCC AGACCCGCCA CTGGGTCATC CGCAGCCTCC TGTCCATTTT GCAGCGCAGC 
AGTGAAAGTG AGTTATGCAT TGAGACACCT AAGCTCTCCA CAAGTGAAGA AAGGGGCAAG 
AAATCAAGCA AGAGCTGTGC ATCAAGCAGT CATGAGAACC GTCCCCTGGA TCTGCTCCAC 
AAGATGGAGT CCAAAAGTTC CAATCAGCTC TCCTGGCTCT CAGTATCAAT GGATGCTGCT 
TTAGGCTGTA GGACTAACAT ATTCCAGATT CAACGTTCTG GAGGGCGTAA ACATACAGAG 
AAGCATGCAA GCAGTGGTTC CACTGTCCAC ATCCACCCCC AGGCTGCTCC TGTTGTTTGC 
AGACATGTTT TGGATACACT CATTCAGTTG GCCAAGGTGT TTCCCAGCCA CTTTACACAG 
CAGCGGACCA AAGAAACAAA CTGTGAGAGT GATCGGGAAC GGGGCAGTAA GCAGGCCTGC 
AGCCCATGCT CCTCACAGTC CTCCAGCAGT GGCATCTGTA CAGACTTCTG GGACTTATTG 
GTAAAACTGG ACAACATGAA TGTTAGCCGA AAAGGCAAGA ACTCCGTGAA GTCAGTACCA 
GTGAGCTCTG GTGGTGAAGG GGAAACTTCC CCACACAGCC TTGAAGCCTC TCCACTGGGG 
CAGCTCATGA ACATGTTGTC ACATCCAGTC ATCCGCCGGA GTTCTCTCTT AACTGAGAAA 
CTCCTGAGAC TCCTCTCTCT CATCTCAATT GCTCTCCCAG AAAACAAAGT ATCAGAAGTG 
CAAACTAACT CCAGCAACAG TGGTTCCTCC ACTGCTGCCA CCTCAAACAC ATCTACTACT 
ACCACCACCA CCACTACTGC CACTGCCCCC ACGCCCACAC CCCCAGCTGC AACTACCCCT 
GTCACTTCTG CTCCAGCTCT GGTTGCTGCC ACAGCTATTT CCACCATTAC TGTAGCTGCT 
TCTACCACAG TGACTACCCC CACAACTGCT ACCACTACTG TTTCAACTTC TACTACTAAA 
GGCAGCAAAT CTCCAGCAAA GGTTGGTGAA GGAGGCAGTG GTATAGACTT CAAAATGGTG 
TCCTCTGGCC TCACTGAAAA CCAGCTACAG CTCTCTGTAG AGGTGTTGAC ATCCCACTCT 
TGTTCTGAAG AAGGCTTGGA AGATGCGGCC AATGTGTTAC TGCAGCTCTC TCGTGGGGAC 
TCTGGTACCC GGGACACTGT TCTTAAGCTG CTACTAAATG GAGCCCGCCA TCTGGGTTAT 
ACCCTCTGTA AACAGATAGG TACCCTACTC GCTGAACTAC GGGAATATAA TCTGGAGCAG 
CAGCGGCGAG CCCAATGCGA GACCCTCTCT CCTGATGGCC TACCTGAGGA GCAACCACAG 
ACCACCAAAC TGAAAGGCAA AATGCAGAGC AGGTTTGACA TGGCTGAAAA TGTGGTAATT 
GTGGCATCTC AGAAGCGACC CTTGGGTGGC CGGGAGCTCC AGCTGCCTTC TATGTCCATG 
TTGACATCCA AGACATCTAC CCAGAAGTTC TTCTTGAGGG TACTACAGGT CATCATCCAG 
CTGCGGGATG ACACACGACG AGCTAACAAG AAAGCCAAGC AGACAGGCAG GCTAGGTTCC 
TCCGGTTTAG GCTCAGCTAG CAGCATCCAG GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT 
GATGCCATTA TACAAATGGT ACGTGAGGGT CAAAGGGCGC GGAGACAGCA ACAAGCAGCA 
ACGTCGGAGT CTTCTAACCA GTCAGAGACA TCTGTCCGGA GAGAGGAATC GCCCATGGAT 
GTGGACCAGC CATCTCCTAG TGCTCAAGAT ACTCAGTCCA TTGTAATCTC AGATGGAACA 
CCACAGGGAG AGAAGGAGAA AGAGGAGAAA CCACCTGAGT TACCCCTGCT CAGTGAGCAA 
CTGAGCCTAG ATGAACTGTG GGACATGCTA GGGGAATGTT TAAAAGAACT GGAGGAATCT 
CATGACCAGC ATGCAGTATT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TTTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGATACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGATG AACCTCCTCC ACTCTCCCCC GCCCCCTTAA CCCCAGCCAC TCCATCCTCC 
CTTGACCCAT TCTTCTCCAG GGAGCCCTCA TCTATGCACA TTTCCTCGAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATACTA 
CGGCAGTCTA CCACCCATCT TGCTGATGGG CCTTTTGCTG TCTTGGTAGA TTATATCCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAA 
GGGCTCCGGA AAGAAGATAT GGCAGTGCAT GTCCGCCGTG ACCATGTATT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCTCCTGAA GAAATGAAGA ATCGATTGTA TATAGTGTTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGGGGG CTCCTGCGAG AGTGGTACAT GATCATCTCT 
CGAGAGATGT TCAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA CCGGGTCACC 
TACACCATCA ATCCATCTTC CCACTGTAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT 
GGACGAATTG TAGCCAAAGC TGTATATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG 
TCTTTCTACA AACACATCTT GGGCAAATCT GTCAGGTATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAGGGCCT AGTTTATCTG CTTGAAAATG ATGTCTCTAC ATTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTTCAAGAA TTTGGAGTAT GTGAAGTTCG TGACCTCAAA 
CCCAATGGAG CCAACATCTT GGTAACAGAG GAGAACAAGA AGGAATATGT ACACCTGGTT 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CAGCCTTCTT GGAGGGCTTC 
TATGAAATCA TTCCAAAGCG CCTCATATCC ATCTTTACTG AGCAGGAATT AGAGCTGCTC 
ATATCAGGGC TGCCTACCAT CGACATTGAT GATCTAAAAT CTAACACTGA GTACCACAAG 
TACCAATCCA ACTCTATTCA GATCCAGTGG TTTTGGAGAG CATTGCGTTC CTTTGACCAA 
GCAGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTACAAGGA 
TTTGCTGCCC TTGAAGGTAT GAATGGCATT CAGAAGTTTC AGATTCATCG AGATGACAGA 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AACTGGACCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAATG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529014.1
CDS	1243..14157
Translation	MSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_006528951.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528951.2

ORF Insert Sequence:

ATGTCATGGA TGCTTGTATG TGATAGGCCA GAAAAAGAAC AGCTGAAAAT GCTTCTATTG 
GCTGTATTGA ACTTCACAGC CTTGCTCATT GAGTACAGCT TTTCTCGGCA TCTGTACAGT 
TCCATAGAGC ATTTGACAAC TTTATTGGCT TCCTCTGATA TGCAAGTGGT GCTGGCAGTC 
CTTAACCTTC TATATGTATT TAGCAAAAGA TCAAACTATA TCACACGTCT TGGATCTGAC 
AAGAGGACTC CGCTACTGAC ACGGCTGCAG CATTTGGCAG AGAGCTGGGG TGGAAAGGAG 
AATGGCTTTG GACTTGCAGA ATGTTGCAGA GACTTGCAAA TGTTGAAATA TCCACCCAGT 
GCAACCACAC TACACTTTGA ATTTTATGCA GATCCTGGAG CTGAGGTCAA AATTGAGAAG 
AGGACAACTA GTAATACACT ACATTATATT CACATAGAAC AACTTGACAA GATTTCAGAA 
AGTCCTTCTG AAATAATGGA ATCTCTTACC AAAATGTACA GCATTCCTAA GGATAAGCAG 
ATGCTGTTAT TTACACATAT ACGACTGGCC CATGGCTTTT CTAATCACAG GAAGCGGTTA 
CAAGCTGTTC AGGCCAGACT GCATGCAATA TCTATATTAG TCTATTCTAA TGCCTTGCAA 
GAGTCAGCAA ACAGTATCTT GTATAATGGG TTGATAGAGG AGTTGGTAGA TGTCCTTCAA 
ATAACAGATA AGCAGCTCAT GGAAATTAAA GCTGCTTCTT TACGAACGTT AACATCAATT 
GTTCATTTGG AGAGAACTCC CAAGCTCAGC AGTATTATTG ACTGTACTGG AACTGCCTCC 
TACCATGGAT TTTTGCCTGT ACTTGTGCGG AACTGCATTC AGGCCATGAT TGATCCTTCC 
ATGGACCCAT ACCCCCATCA GTTTGCCACA GCTCTTTTCT CCTTTTTGTA CCATCTGGCT 
AGCTACGATG CTGGTGGTGA AGCCTTGGTC TCCTGTGGAA TGATGGAAGC CTTACTAAAG 
GTCATAAAGT TTCTTGGTGA TGAACAAGAC CAGATAACAT TTGTAACCAG AGCTGTCAGA 
GTGGTTGACC TTATCACCAA CCTGGACATG GCAGCTTTTC AGTCTCATAG TGGTCTTTCT 
ATCTTCATCT ATAGACTTGA GCACGAAGTA GATTTGTGTC GTAAAGAATG CCCTTTCGTG 
ATCAAGCCAA AGATCCAGAG ACCCAGTACC ACTCAAGAAG GAGAAGAAAT GGAAACTGAT 
ATGGATGGAG TTCAGTGTAT TCCACAACGA GCAGCACTTC TCAAGTCCAT GTTGAATTTC 
CTCAAGAAGG CCATTCAAGA CCCTGCTTTC TCAGATGGCA TACGACATGT GATGGATGGT 
TCTTTACCTA CCTCTCTGAA ACACATCATC AGCAATGCAG AATACTATGG TCCATCACTC 
TTCCTCCTAG CTACTGAAGT GGTGACTGTG TTTGTATTTC AAGAGCCATC ACTGCTGTCC 
TCACTCCAGG ACAATGGACT GACAGATGTC ATGCTGCATG CACTGCTCAT CAAAGATGTT 
CCTGCTACAA GGGAAGTCCT CGGTTCCCTC CCAAATGTAT TCAGTGCACT TTGCTTGAAT 
GCCCGAGGTC TTCAGTCTTT TGTACAATGT CAGCCATTTG AACGTCTCTT CAAAGTTCTT 
CTGTCTCCTG ATTACCTCCC AGCCATGCGG AGGAGGAGGA GTTCTGATCC CCTTGGGGAT 
ACTGCATCCA ACCTGGGGAG TGCTGTTGAT GAGCTCATGA GACACCAGCC TACCCTCAAA 
ACAGATGCAA CAACTGCCAT CATCAAGTTA CTTGAAGAAA TCTGTAATCT TGGAAGAGAT 
CCTAAATACA TTTGTCAGAA GCCATCTATT CAGAAAGCGG ATGGTACTGC TACTGCTCCT 
CCTCCAAGGT CTAATCATGC TGCAGAAGAG GCTTCCAGTG AAGATGAGGA GGAAGAGGAA 
GTACAGGCCA TGCAGAGCTT TAATTCTGCC CAGCAGAATG AAACTGAGCC TAATCAGCAG 
GTCGTTGGTA CCGAGGAACG TATACCTATA CCCCTCATGG ATTACATCCT TAATGTGATG 
AAATTTGTGG AATCTATTTT GAGCAACAAT ACAACAGATG ACCACTGTCA AGAATTTGTA 
AACCAGAAAG GACTCTTACC TTTGGTTACC ATTTTGGGTC TTCCCAATCT GCCCATTGAC 
TTTCCCACAT CTGCTGCCTG TCAGGCTGTT GCAGGTGTCT GCAAATCCAT ATTGACCCTG 
TCACATGAAC CCAAAGTTCT TCAAGAAGGT CTCCTTCAAT TAGACTTGAT CCTTTCCTCC 
TTGGAGCCTT TACATCGGCC GATTGAATCC CCTGGGGGCT CAGTGTTACT GAGAGAATTG 
GCATGTGCAG GCAATGTTGC TGATGCTACC CTCTCAGCTC AGGCCACGCC TCTGTTGCAT 
GCACTCACTG CAGCCCATGC TTACATCATG ATGTTTGTTC ACACTTGCAG AGTTGGACAG 
AGTGAAATTC GTTCTATCTC AGTAAACCAG TGGGGTTCAC AATTGGGTTT GAGTGTTCTG 
AGCAAGCTAA GCCAGTTATA CTGTTCTCTG GTGTGGGAAA GCACTGTCCT CCTCTCTCTA 
TGTACCCCCA ACAGCCTACC ATCTGGATGT GAATTTGGTC AAGCGGACAT GCAGAAACTA 
GTTCCAAAGG ATGAGAAGGC AGGTACGACC CAGGGCGGAA AAAGATCAGA TGGAGAACAG 
GATGGAACTG CTGGAAGCAT GGATGCATCT GCCCAGGGTT TATTAGAAGG AATTGAACTA 
GATGGTGACA CACTGGCACC CATGGAGACA GATGAACCTT CTTCTTCAGA TTCTAAGGGC 
AAATCTAAAA TCACACCTGC CATGGCTGCA AGAATTAAAC AAATCAAGCC GTTGCTGTCA 
GCTTCTTCCA GATTAGGCCG AGCACTTGCT GAGCTCTTTG GACTCCTTGT TAAGCTTTGT 
GTGGGTTCTC CTGTCCGCCA AAGGAGGAGC CATCATGCTG CCAGTACCAC CACTGCACCA 
ACACCTGCTG CTAGATCAAC AGCTTCAGCC CTCACTAAGC TACTCACAAA AGGCTTATCA 
TGGCAGCCCC CACCATATAC ACCCACTCCC CGCTTCAGGC TGACGTTCTT CATCTGTTCA 
GTTGGATTCA CCTCTCCAAT GCTCTTTGAT GAGAGAAAGT ATCCTTACCA CCTTATGCTG 
CAGAAATTTC TCTGCTCTGG TGGTCACAAT GCTCTTTTTG AAACTTTCAA CTGGGCTCTA 
TCCATGGGAG GAAAAGTTCC TGTTTCTGAG GGATTGGAAC ATTCAGATCT ACCTGATGGT 
ACAGGAGAAT TCCTAGATGC CTGGCTTATG CTTGTAGAAA AGATGGTAAA TCCCACCACG 
GTGCTTGAAT CTCCACATTC GTTGCCTGCT AAATTGCCTG GAGGAGTCCA GAGCTTTCCA 
CAATTTAGTG CCCTTCGCTT CCTTGTAGTA ACTCAAAAAG CAGCCTTTAC TTGTATCAAG 
AACCTGTGGA ACCGGAAACC CCTTAAAGTA TATGGGGGAA GAATGGCTGA GTCTATGCTG 
GCCATTTTGT GCCACATTCT CCGAGGGGAA CCTGTGATTC GAGAAAGACT GAGCAAAGAG 
AAGGAAGGAT CTCGAGGAGA AGAAGAAGCA GGCCAGGAAG AAGGTGGCTC ACGCAGAGAA 
CCTCAAGTCA ACCAGCAACA GCTACAACAG CTCATGGACA TGGGTTTCAC AAGAGAACAT 
GCCATGGAGG CACTGTTGAA TACCAGTACG ATGGAACAAG CAACAGAGTA CCTTTTAACC 
CACCCTCCTC CAATCATAGG AGGAGTTGTT CGGGATCTTA GCATGTCAGA AGAGGACCAG 
ATGATGAGAG CAATTGCTAT GTCTCTGGGA CAGGATATTC CAATGGATCA AAGGGCAGAG 
TCACCTGAGG AAGTTGCTTG CCGGAAGGAG GAAGAAGAGC GTAAAGCTCG AGAAAAACAA 
GAAGAGGAAG AAGCTAAATG TCTAGAGAAG TTTCAAGATG CTGACCCCTT GGAACAAGAT 
GAGCTCCACA CTTTCACAGA TACAATGTTA CCAGGCTGCT TCCATCTTCT TGATGAGTTG 
CCTGATACAG TGTACCGTGT TTGTGATCTG ATCATGACAG CTATCAAACG TAATGGAGCT 
GACTATCGTG ATATGATTCT AAAGCAAGTT GTCAACCAGG TGTGGGAAGC TGCTGATGTA 
TTGATCAAAG CTGCTCTTCC TCTAACAACA AGTGACACAA AAACAGTTTC AGAGTGGATC 
AGTCAGATGG CTACCCTGCC ACAGGCCTCC AATTTGGCTA CTAGAATCTT GCTTTTAACA 
CTACTTTTTG AGGAGTTGAA GTTACCTTGT GCCTGGGTGG TTGAATCAAG TGGCATTCTT 
AATGTCCTAA TCAAACTCTT AGAAGTGGTT CAACCCTGCC TACAGGCTGC CAAAGAGCAA 
AAGGAAGTCC AGACCCCAAA GTGGATCACC CCAGTGTTGC TCCTTATTGA TTTTTATGAA 
AAGACAGCCA TCTCATCAAA GAGAAGAGCC CAAATGACTA AGTACCTACA GTCCAACAGC 
AACAACTGGC GCTGGTTTGA TGACCGCTCT GGGCGTTGGT GTAGTTACAG TGCTAGCAAC 
AACAGCACCA TTGATTCTGC CTGGAAATCT GGAGAAACAA GTGTAAGATT CACTGCTGGC 
CGACGAAGAT ACACTGTCCA GTTCACTACA ATGGTGCAGG TTAATGAAGA AACAGGAAAC 
CGACGCCCTG TGATGCTGAC TCTCCTCAGG GTGCCACGAC TGAGTAAGAA CTCAAAAAGC 
AGCAATGGAC AGGAACTAGA GAAGACACTG GAAGAAAGCA AGGAGACCGA TATCAAACAT 
AAAGAAAATA AAGGCAATGA TATCCCCTTA GCTCTAGAGA GCACAAACAC TGAAAAAGAG 
GCAAGCCTGG ATGAAACAAA AATTGGGGAG ATCCTAATCC AGGGCCTGAC AGAAGATATG 
GTGACTGTTC TAATCCGTGC CTGTGTGAGC ATGCTGGGAG TCCCTGTGGA CCCAGACACT 
TTGCATGCCA CCCTTCGTCT CTGTTTAAGG CTCACCCGAG ACCACAAATA TGCCATGATG 
TTTGCAGAAC TGAAGAGTAC ACGAATGATC TTGAATTTGA CCCAGAGCTC AGGTTTCAAT 
GGGTTTACCC CCTTAGTCAC CCTTCTCTTA AGGCACATCA TTGAGGATCC TTGTACTCTT 
CGTCATACTA TGGAAAAGGT TGTTCGCTCG GCAGCCACAA GTGGAGCTGG TAGTACTACA 
TCTGGTGTTG TATCTGGCAG CCTCGGTTCT CGGGAGATCA ACTATATCCT TAGAGTCCTA 
GGACCAGCTG CCTGCCGCAA TCCAGACATA TTCACAGAAG TAGCCAATTG CTGTATCCGC 
ATTGCCCTTC CAGCCCCTCG AGGCTCAGGA ACTGCTTCAG ATGATGAATT TGAGAATCTT 
AGAATTAAAG GTCCTAATGC TGTACAGCTG GTAAAGACAA CACCTTTGAA GCCCTCATCT 
CTGCCTGTCA TTCCTGATAC TATTAAGGAA GTTATCTATG ATATGCTGAA TGCTTTGGCT 
GCATACCATG CTCCAGAGGA AGCAGACAAA TCTGATCCTA AACCTGGGGG AACGACCCAA 
GAGGTTGGCC AGCTCCTGCA AGACATGGGT GACGATGTAT ACCAGCAGTA CCGGTCACTT 
ACTCGTCAGA GCAGTGACTT TGATACACAG TCAGGTTTTT CCTTAAATAG TCAGGTTTTT 
GCTGCAGATG GTGCCCCTGC TGAAACTTCC ACAACTGGAA CCTCCCAAGG AGAGGGAGCT 
TCAACTCCAG AGGAGACTCG AGAAGGGAAG AAAGATAAAG AAGGGGACCG TACATCTGAG 
GAAGGCAAGC AGAAAAGCAA GGGCAGCAAA CCTTTAATGC CTACCTCTAC TATCCTTCGT 
CTTCTGGCAG AGTTGGTGAG GTCCTATGTT GGTATTGCTA CCCTGATTGC AAACTACAGC 
TATACCGTGG GCCAGTCTGA GCTGATCAAA GAGGACTGCA GTGTGCTAGC TTTTGTCCTG 
GACCACCTCC TCCCACACAC CCAGAATGCA GAAGACAAGG ATACACCTGC CCTGGCACGT 
CTATTCCTTG CAAGTCTGGC TGCTGCGGGG AGTGGCACAG ATGCTCAAGT GGCCCTAGTG 
AATGAAGTGA AAGCAGCCCT GGGACGGGCG CTTGCTATGG CTGAGAGTAC AGAGAAGCAC 
GCCAGACTTC AGGCAGTGAT GTGTATCATC AGTACTATCA TGGAATCCTG CCCCTCCACC 
TCCAGCTTCT ACAGTAGTGC TACAGCCAAG ACTCAGCATA ATGGCATGAA CAACATCATT 
CGACTGTTCC TAAAGAAGGG ACTAGTCAAT GACCTGGCCA GAGTTCCTCA CAGCCTAGAC 
CTGTCCAGTC CCAATATGGC CAACACAGTC AATGCTGCTC TAAAACCCTT GGAAACACTT 
TCCCGGATTG TAAATCAACC CAGTAGCCTT TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC 
AAGTCTGAGC AGGATGCTCA AGGAGCTTCT CAGGACTCCA GCAGCCATCA GCAAGATCCA 
GGCGAGCCTG GGGAGGCAGA AGTGCAGGAG GAGGATCATG ATGTCACTCA GACAGAGGTG 
GCAGATGGGG ATATCATGGA TGGGGAGGCT GAAACCGACT CAGTGGTGAT TGCCGGGCAG 
CCTGAGGTGC TCAGTTCACA AGAGATGCAG GTTGAAAATG AACTGGAGGA CCTGATAGAT 
GAGTTGCTTG AGAGGGATGG CGGATCTGGG AACAGTACAA TTATAGTGAG CAGAAGTGGA 
GAGGATGAAT CACAAGAGGA CGTGCTGATG GATGAAGCTC CTTCCAACCT CAGCCAAGCT 
TCCACCTTGC AGGCCAACCG AGAAGATTCC ATGAATATCC TGGACCCTGA GGATGAGGAG 
GAACATACTC AGGAAGAGGA CAGCAGTGGC AGTAACGAGG ATGAGGATGA TAGTCAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGAGGAA GATGATCAGG AGGATGATGA AGGTGAAGAA 
GGAGATGAAG ATGATGACGA CGATGGTTCT GAGATGGAAT TGGATGAGGA TTACCCTGAT 
ATGAACGCTT CTCCCTTGGT CCGATTTGAG CGCTTTGACC GGGAGGATGA TCTCATCATT 
GAGTTTGACA ACATGTTCTC CAGTGCTACA GACATTCCCC CATCCCCAGG AAATATCCCT 
ACCACCCACC CATTGATGGT ACGTCATGCA GATCACAGTT CCCTGACACT GGGCAGTGGT 
TCTTCCACAA CTCGTCTTAC CCAGGGCATC GGTCGCAGTC AAAGGACCCT CAGACAGCTC 
ACTGCCAATA CAGGCCATAC CATTCATGTT CACTACCCTG GGAATCGCCA GCCTAACCCT 
CCTCTTATAC TCCAGAGGTT GCTTGGTCCC TCTGCAGCTG CTGACATTCT TCAGCTGAGC 
AGCAGCCTTC CATTACAAAG CCGAGGCCGG GCCCGCCTCC TAGTGGGCAA TGATGACGTC 
CATATCATCG CCAGGTCTGA TGACGAGCTG CTGGATGACT TTTTTCATGA CCAGAGTACA 
GCCACTAGCC AAGCAGGAAC CCTGTCCAGC ATCCCAACAG CTCTAACCCG ATGGACAGAG 
GAATGCAAAG TTCTTGATGC TGAGAGTATG CATGACTGTG TTTCAGTGGT TAAAGTGCCC 
ATTGTCAATC ACCTGGAATT CTTGAGAGAT GAGGAGCTAG AAGAGAGAAG GGAGAAACGC 
AGGAAACAAT TGGCTGAGGA AGAAACAAAA ATAATAGACA AAGGCAAAGA AGACAAGGAG 
AACAGAGATC AGAGTGCACA GTGTACTGTA ACTAAGACAA ATGACTCCAC TGAACAGAAT 
GTCTCAGATG GGACTCCTAT GCCTGACAGC TATCCAACAA CCCCCTCTTC AACGGATGCA 
CCTACATCTG AGTCCAAGGA AACACTTGGT ACCCTACAAC CCTCCCAGCA ACAGCCAGCA 
CTTCCACCCC CACCATCCTT AGGAGAGATT CCTCAGGAAC TACAGTCCCC TGCTGAAGAA 
GTGGCAAACT CTACACAGCT ATTGATGCCT ATAGAGTTGG AGGAATTGGG TCCCACAAGA 
CCAAGTGGAG AAGCAGAAAC AACTCAAATG GAATTGTCTC CAGCTCCCAC CATAACCTCT 
CTTTCCCCAG AGAGAGCTGA AGATTCAGAC GCGTTAACAG CTGTCAGCAG TCAGCTGGAA 
GGTTCTCCCA TGGACACCAG CAGCCTGGCT TCCTGTACGT TAGAAGAGGC TGTGGGTGAC 
ACTCCAGCAG CTGGCAGTTC TGAGCAGCCC ACAGCAGGTA GTTCCACTCC TGGGGATGCG 
CCATCAGTTG TGGCAGAAGT GCAAGGCAGA CCTGATGTAT CAAGAGAATC CAACCAGCCA 
CCTGAGGACA GCTCCCCCCC TGCATCCTCG GAGAGTTCTT CCACTAGAGA TTCTGCCGTG 
GCCATCTCTG GAGCAGATTC TCGTGGAATT CTAGAAGAGC CACTACCTTC AACAAGCAGT 
GAAGAAGAAG ATCCCCTTGC TGGTATCAGT CTCCCTGAAG GTGTGGATCC CTCCTTTCTG 
GCTGCTCTCC CTGATGATAT CCGTCGGGAA GTCCTACAGA ACCAGCTGGG CATACGTCCA 
CCAACCCGAA GTGCTCCCTC CTCAAATAGC TCAGCTCCTG CAGTAGTGGG GAACCCTGGT 
GTAACTGAAG TGAGCCCTGA GTTCCTGGCT GCCCTACCTC CAGCTATTCA AGAGGAAGTG 
CTAGCACAGC AGAGAGCTGA ACAGCAGCGA CGGGAACTGG CTCAGAATGC GAGCTCAGAC 
ACCCCCATGG ACCCTGTGAC TTTCATTCAG ACTTTGCCCT CAGATCTGCG CCGTAGTGTC 
CTAGAGGATA TGGAAGACAG TGTGTTAGCT GTGATGCCAC CTGACATTGC AGCTGAGGCT 
CAAGCTCTAA GACGAGAACA AGAAGCCCGA CAGCGGCAGC TCATGCATGA GCGTCTTTTT 
GGACACAGTA GTACTTCTGC ACTCTCTGCC ATTCTCCGAA GCCCGGCTTT TACCAGTCGC 
CTGAGTGGCA ACCGTGGGGT CCAGTATACT CGCCTTGCTG TACAGAGGGG TGGCACCTTC 
CAGATGGGGG GTAGCAGCAG CCATAATAGG CCTTCTGGCA GTAATGTGGA CACACTACTC 
CGCCTCCGAG GACGACTCCT TCTAGACCAT GAAGCCCTGT CTTGCCTCTT GGTCCTACTT 
TTTGTGGATG AGCCAAAGCT CAATACTAGC CGTCTACACC GAGTACTGAG AAATCTCTGC 
TATCATGCCC AGACCCGCCA CTGGGTCATC CGCAGCCTCC TGTCCATTTT GCAGCGCAGC 
AGTGAAAGTG AGTTATGCAT TGAGACACCT AAGCTCTCCA CAAGTGAAGA AAGGGGCAAG 
AAATCAAGCA AGAGCTGTGC ATCAAGCAGT CATGAGAACC GTCCCCTGGA TCTGCTCCAC 
AAGATGGAGT CCAAAAGTTC CAATCAGCTC TCCTGGCTCT CAGTATCAAT GGATGCTGCT 
TTAGGCTGTA GGACTAACAT ATTCCAGATT CAACGTTCTG GAGGGCGTAA ACATACAGAG 
AAGCATGCAA GCAGTGGTTC CACTGTCCAC ATCCACCCCC AGGCTGCTCC TGTTGTTTGC 
AGACATGTTT TGGATACACT CATTCAGTTG GCCAAGGTGT TTCCCAGCCA CTTTACACAG 
CAGCGGACCA AAGAAACAAA CTGTGAGAGT GATCGGGAAC GGGGCAGTAA GCAGGCCTGC 
AGCCCATGCT CCTCACAGTC CTCCAGCAGT GGCATCTGTA CAGACTTCTG GGACTTATTG 
GTAAAACTGG ACAACATGAA TGTTAGCCGA AAAGGCAAGA ACTCCGTGAA GTCAGTACCA 
GTGAGCTCTG GTGGTGAAGG GGAAACTTCC CCACACAGCC TTGAAGCCTC TCCACTGGGG 
CAGCTCATGA ACATGTTGTC ACATCCAGTC ATCCGCCGGA GTTCTCTCTT AACTGAGAAA 
CTCCTGAGAC TCCTCTCTCT CATCTCAATT GCTCTCCCAG AAAACAAAGT ATCAGAAGTG 
CAAACTAACT CCAGCAACAG TGGTTCCTCC ACTGCTGCCA CCTCAAACAC ATCTACTACT 
ACCACCACCA CCACTACTGC CACTGCCCCC ACGCCCACAC CCCCAGCTGC AACTACCCCT 
GTCACTTCTG CTCCAGCTCT GGTTGCTGCC ACAGCTATTT CCACCATTAC TGTAGCTGCT 
TCTACCACAG TGACTACCCC CACAACTGCT ACCACTACTG TTTCAACTTC TACTACTAAA 
GGCAGCAAAT CTCCAGCAAA GGTTGGTGAA GGAGGCAGTG GTATAGACTT CAAAATGGTG 
TCCTCTGGCC TCACTGAAAA CCAGCTACAG CTCTCTGTAG AGGTGTTGAC ATCCCACTCT 
TGTTCTGAAG AAGGCTTGGA AGATGCGGCC AATGTGTTAC TGCAGCTCTC TCGTGGGGAC 
TCTGGTACCC GGGACACTGT TCTTAAGCTG CTACTAAATG GAGCCCGCCA TCTGGGTTAT 
ACCCTCTGTA AACAGATAGG TACCCTACTC GCTGAACTAC GGGAATATAA TCTGGAGCAG 
CAGCGGCGAG CCCAATGCGA GACCCTCTCT CCTGATGGCC TACCTGAGGA GCAACCACAG 
ACCACCAAAC TGAAAGGCAA AATGCAGAGC AGGTTTGACA TGGCTGAAAA TGTGGTAATT 
GTGGCATCTC AGAAGCGACC CTTGGGTGGC CGGGAGCTCC AGCTGCCTTC TATGTCCATG 
TTGACATCCA AGACATCTAC CCAGAAGTTC TTCTTGAGGG TACTACAGGT CATCATCCAG 
CTGCGGGATG ACACACGACG AGCTAACAAG AAAGCCAAGC AGACAGGCAG GCTAGGTTCC 
TCCGGTTTAG GCTCAGCTAG CAGCATCCAG GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT 
GATGCCATTA TACAAATGGT ACGTGAGGGT CAAAGGGCGC GGAGACAGCA ACAAGCAGCA 
ACGTCGGAGT CTTCTAACCA GTCAGAGACA TCTGTCCGGA GAGAGGAATC GCCCATGGAT 
GTGGACCAGC CATCTCCTAG TGCTCAAGAT ACTCAGTCCA TTGTAATCTC AGATGGAACA 
CCACAGGGAG AGAAGGAGAA AGAGGAGAAA CCACCTGAGT TACCCCTGCT CAGTGAGCAA 
CTGAGCCTAG ATGAACTGTG GGACATGCTA GGGGAATGTT TAAAAGAACT GGAGGAATCT 
CATGACCAGC ATGCAGTATT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TTTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGATACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGATG AACCTCCTCC ACTCTCCCCC GCCCCCTTAA CCCCAGCCAC TCCATCCTCC 
CTTGACCCAT TCTTCTCCAG GGAGCCCTCA TCTATGCACA TTTCCTCGAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATACTA 
CGGCAGTCTA CCACCCATCT TGCTGATGGG CCTTTTGCTG TCTTGGTAGA TTATATCCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAA 
GGGCTCCGGA AAGAAGATAT GGCAGTGCAT GTCCGCCGTG ACCATGTATT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCTCCTGAA GAAATGAAGA ATCGATTGTA TATAGTGTTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGGGGG CTCCTGCGAG AGTGGTACAT GATCATCTCT 
CGAGAGATGT TCAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA CCGGGTCACC 
TACACCATCA ATCCATCTTC CCACTGTAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT 
GGACGAATTG TAGCCAAAGC TGTATATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG 
TCTTTCTACA AACACATCTT GGGCAAATCT GTCAGGTATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAGGGCCT AGTTTATCTG CTTGAAAATG ATGTCTCTAC ATTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTTCAAGAA TTTGGAGTAT GTGAAGTTCG TGACCTCAAA 
CCCAATGGAG CCAACATCTT GGTAACAGAG GAGAACAAGA AGGAATATGT ACACCTGGTT 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CAGCCTTCTT GGAGGGCTTC 
TATGAAATCA TTCCAAAGCG CCTCATATCC ATCTTTACTG AGCAGGAATT AGAGCTGCTC 
ATATCAGGGC TGCCTACCAT CGACATTGAT GATCTAAAAT CTAACACTGA GTACCACAAG 
TACCAATCCA ACTCTATTCA GATCCAGTGG TTTTGGAGAG CATTGCGTTC CTTTGACCAA 
GCAGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTACAAGGA 
TTTGCTGCCC TTGAAGGTAT GAATGGCATT CAGAAGTTTC AGATTCATCG AGATGACAGA 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AACTGGACCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAATG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_011247854.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246156.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X13, mRNA.

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CloneID	OMu69810
Clone ID Related Accession (Same CDS sequence)	XM_011247854.1 , XM_030251443.1
Accession Version	XM_011247854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGAC 
AAATCTGATC CTAAACCTGG GGGAACGACC CAAGAGGTTG GCCAGCTCCT GCAAGACATG 
GGTGACGATG TATACCAGCA GTACCGGTCA CTTACTCGTC AGAGCAGTGA CTTTGATACA 
CAGTCAGGTT TTTCCTTAAA TAGTCAGGTT TTTGCTGCAG ATGGTGCCCC TGCTGAAACT 
TCCACAACTG GAACCTCCCA AGGAGAGGGA GCTTCAACTC CAGAGGAGAC TCGAGAAGGG 
AAGAAAGATA AAGAAGGGGA CCGTACATCT GAGGAAGGCA AGCAGAAAAG CAAGGGCAGC 
AAACCTTTAA TGCCTACCTC TACTATCCTT CGTCTTCTGG CAGAGTTGGT GAGGTCCTAT 
GTTGGTATTG CTACCCTGAT TGCAAACTAC AGCTATACCG TGGGCCAGTC TGAGCTGATC 
AAAGAGGACT GCAGTGTGCT AGCTTTTGTC CTGGACCACC TCCTCCCACA CACCCAGAAT 
GCAGAAGACA AGGATACACC TGCCCTGGCA CGTCTATTCC TTGCAAGTCT GGCTGCTGCG 
GGGAGTGGCA CAGATGCTCA AGTGGCCCTA GTGAATGAAG TGAAAGCAGC CCTGGGACGG 
GCGCTTGCTA TGGCTGAGAG TACAGAGAAG CACGCCAGAC TTCAGGCAGT GATGTGTATC 
ATCAGTACTA TCATGGAATC CTGCCCCTCC ACCTCCAGCT TCTACAGTAG TGCTACAGCC 
AAGACTCAGC ATAATGGCAT GAACAACATC ATTCGACTGT TCCTAAAGAA GGGACTAGTC 
AATGACCTGG CCAGAGTTCC TCACAGCCTA GACCTGTCCA GTCCCAATAT GGCCAACACA 
GTCAATGCTG CTCTAAAACC CTTGGAAACA CTTTCCCGGA TTGTAAATCA ACCCAGTAGC 
CTTTTTGGCA GCAAGAGTGC TTCTAGCAAG AACAAGTCTG AGCAGGATGC TCAAGGAGCT 
TCTCAGGACT CCAGCAGCCA TCAGCAAGAT CCAGGCGAGC CTGGGGAGGC AGAAGTGCAG 
GAGGAGGATC ATGATGTCAC TCAGACAGAG GTGGCAGATG GGGATATCAT GGATGGGGAG 
GCTGAAACCG ACTCAGTGGT GATTGCCGGG CAGCCTGAGG TGCTCAGTTC ACAAGAGATG 
CAGGTTGAAA ATGAACTGGA GGACCTGATA GATGAGTTGC TTGAGAGGGA TGGCGGATCT 
GGGAACAGTA CAATTATAGT GAGCAGAAGT GGAGAGGATG AATCACAAGA GGACGTGCTG 
ATGGATGAAG CTCCTTCCAA CCTCAGCCAA GCTTCCACCT TGCAGGCCAA CCGAGAAGAT 
TCCATGAATA TCCTGGACCC TGAGGATGAG GAGGAACATA CTCAGGAAGA GGACAGCAGT 
GGCAGTAACG AGGATGAGGA TGATAGTCAG GATGAAGAGG AGGAGGAGGA GGAAGATGAG 
GAAGATGATC AGGAGGATGA TGAAGGTGAA GAAGGAGATG AAGATGATGA CGACGATGGT 
TCTGAGATGG AATTGGATGA GGATTACCCT GATATGAACG CTTCTCCCTT GGTCCGATTT 
GAGCGCTTTG ACCGGGAGGA TGATCTCATC ATTGAGTTTG ACAACATGTT CTCCAGTGCT 
ACAGACATTC CCCCATCCCC AGGAAATATC CCTACCACCC ACCCATTGAT GGTACGTCAT 
GCAGATCACA GTTCCCTGAC ACTGGGCAGT GGTTCTTCCA CAACTCGTCT TACCCAGGGC 
ATCGGTCGCA GTCAAAGGAC CCTCAGACAG CTCACTGCCA ATACAGGCCA TACCATTCAT 
GTTCACTACC CTGGGAATCG CCAGCCTAAC CCTCCTCTTA TACTCCAGAG GTTGCTTGGT 
CCCTCTGCAG CTGCTGACAT TCTTCAGCTG AGCAGCAGCC TTCCATTACA AAGCCGAGGC 
CGGGCCCGCC TCCTAGTGGG CAATGATGAC GTCCATATCA TCGCCAGGTC TGATGACGAG 
CTGCTGGATG ACTTTTTTCA TGACCAGAGT ACAGCCACTA GCCAAGCAGG AACCCTGTCC 
AGCATCCCAA CAGCTCTAAC CCGATGGACA GAGGAATGCA AAGTTCTTGA TGCTGAGAGT 
ATGCATGACT GTGTTTCAGT GGTTAAAGTG CCCATTGTCA ATCACCTGGA ATTCTTGAGA 
GATGAGGAGC TAGAAGAGAG AAGGGAGAAA CGCAGGAAAC AATTGGCTGA GGAAGAAACA 
AAAATAATAG ACAAAGGCAA AGAAGACAAG GAGAACAGAG ATCAGAGTGC ACAGTGTACT 
GTAACTAAGA CAAATGACTC CACTGAACAG AATGTCTCAG ATGGGACTCC TATGCCTGAC 
AGCTATCCAA CAACCCCCTC TTCAACGGAT GCACCTACAT CTGAGTCCAA GGAAACACTT 
GGTACCCTAC AACCCTCCCA GCAACAGCCA GCACTTCCAC CCCCACCATC CTTAGGAGAG 
ATTCCTCAGG AACTACAGTC CCCTGCTGAA GAAGTGGCAA ACTCTACACA GCTATTGATG 
CCTATAGAGT TGGAGGAATT GGGTCCCACA AGACCAAGTG GAGAAGCAGA AACAACTCAA 
ATGGAATTGT CTCCAGCTCC CACCATAACC TCTCTTTCCC CAGAGAGAGC TGAAGATTCA 
GACGCGTTAA CAGCTGTCAG CAGTCAGCTG GAAGGTTCTC CCATGGACAC CAGCAGCCTG 
GCTTCCTGTA CGTTAGAAGA GGCTGTGGGT GACACTCCAG CAGCTGGCAG TTCTGAGCAG 
CCCACAGCAG GTAGTTCCAC TCCTGGGGAT GCGCCATCAG TTGTGGCAGA AGTGCAAGGC 
AGACCTGATG TATCAAGAGA ATCCAACCAG CCACCTGAGG ACAGCTCCCC CCCTGCATCC 
TCGGAGAGTT CTTCCACTAG AGATTCTGCC GTGGCCATCT CTGGAGCAGA TTCTCGTGGA 
ATTCTAGAAG AGCCACTACC TTCAACAAGC AGTGAAGAAG AAGATCCCCT TGCTGGTATC 
AGTCTCCCTG AAGGTGTGGA TCCCTCCTTT CTGGCTGCTC TCCCTGATGA TATCCGTCGG 
GAAGTCCTAC AGAACCAGCT GGGCATACGT CCACCAACCC GAAGTGCTCC CTCCTCAAAT 
AGCTCAGCTC CTGCAGTAGT GGGGAACCCT GGTGTAACTG AAGTGAGCCC TGAGTTCCTG 
GCTGCCCTAC CTCCAGCTAT TCAAGAGGAA GTGCTAGCAC AGCAGAGAGC TGAACAGCAG 
CGACGGGAAC TGGCTCAGAA TGCGAGCTCA GACACCCCCA TGGACCCTGT GACTTTCATT 
CAGACTTTGC CCTCAGATCT GCGCCGTAGT GTCCTAGAGG ATATGGAAGA CAGTGTGTTA 
GCTGTGATGC CACCTGACAT TGCAGCTGAG GCTCAAGCTC TAAGACGAGA ACAAGAAGCC 
CGACAGCGGC AGCTCATGCA TGAGCGTCTT TTTGGACACA GTAGTACTTC TGCACTCTCT 
GCCATTCTCC GAAGCCCGGC TTTTACCAGT CGCCTGAGTG GCAACCGTGG GGTCCAGTAT 
ACTCGCCTTG CTGTACAGAG GGGTGGCACC TTCCAGATGG GGGGTAGCAG CAGCCATAAT 
AGGCCTTCTG GCAGTAATGT GGACACACTA CTCCGCCTCC GAGGACGACT CCTTCTAGAC 
CATGAAGCCC TGTCTTGCCT CTTGGTCCTA CTTTTTGTGG ATGAGCCAAA GCTCAATACT 
AGCCGTCTAC ACCGAGTACT GAGAAATCTC TGCTATCATG CCCAGACCCG CCACTGGGTC 
ATCCGCAGCC TCCTGTCCAT TTTGCAGCGC AGCAGTGAAA GTGAGTTATG CATTGAGACA 
CCTAAGCTCT CCACAAGTGA AGAAAGGGGC AAGAAATCAA GCAAGAGCTG TGCATCAAGC 
AGTCATGAGA ACCGTCCCCT GGATCTGCTC CACAAGATGG AGTCCAAAAG TTCCAATCAG 
CTCTCCTGGC TCTCAGTATC AATGGATGCT GCTTTAGGCT GTAGGACTAA CATATTCCAG 
ATTCAACGTT CTGGAGGGCG TAAACATACA GAGAAGCATG CAAGCAGTGG TTCCACTGTC 
CACATCCACC CCCAGGCTGC TCCTGTTGTT TGCAGACATG TTTTGGATAC ACTCATTCAG 
TTGGCCAAGG TGTTTCCCAG CCACTTTACA CAGCAGCGGA CCAAAGAAAC AAACTGTGAG 
AGTGATCGGG AACGGGGCAG TAAGCAGGCC TGCAGCCCAT GCTCCTCACA GTCCTCCAGC 
AGTGGCATCT GTACAGACTT CTGGGACTTA TTGGTAAAAC TGGACAACAT GAATGTTAGC 
CGAAAAGGCA AGAACTCCGT GAAGTCAGTA CCAGTGAGCT CTGGTGGTGA AGGGGAAACT 
TCCCCACACA GCCTTGAAGC CTCTCCACTG GGGCAGCTCA TGAACATGTT GTCACATCCA 
GTCATCCGCC GGAGTTCTCT CTTAACTGAG AAACTCCTGA GACTCCTCTC TCTCATCTCA 
ATTGCTCTCC CAGAAAACAA AGTATCAGAA GTGCAAACTA ACTCCAGCAA CAGTGGTTCC 
TCCACTGCTG CCACCTCAAA CACATCTACT ACTACCACCA CCACCACTAC TGCCACTGCC 
CCCACGCCCA CACCCCCAGC TGCAACTACC CCTGTCACTT CTGCTCCAGC TCTGGTTGCT 
GCCACAGCTA TTTCCACCAT TACTGTAGCT GCTTCTACCA CAGTGACTAC CCCCACAACT 
GCTACCACTA CTGTTTCAAC TTCTACTACT AAAGGCAGCA AATCTCCAGC AAAGGTTGGT 
GAAGGAGGCA GTGGTATAGA CTTCAAAATG GTGTCCTCTG GCCTCACTGA AAACCAGCTA 
CAGCTCTCTG TAGAGGTGTT GACATCCCAC TCTTGTTCTG AAGAAGGCTT GGAAGATGCG 
GCCAATGTGT TACTGCAGCT CTCTCGTGGG GACTCTGGTA CCCGGGACAC TGTTCTTAAG 
CTGCTACTAA ATGGAGCCCG CCATCTGGGT TATACCCTCT GTAAACAGAT AGGTACCCTA 
CTCGCTGAAC TACGGGAATA TAATCTGGAG CAGCAGCGGC GAGCCCAATG CGAGACCCTC 
TCTCCTGATG GCCTACCTGA GGAGCAACCA CAGACCACCA AACTGAAAGG CAAAATGCAG 
AGCAGGTTTG ACATGGCTGA AAATGTGGTA ATTGTGGCAT CTCAGAAGCG ACCCTTGGGT 
GGCCGGGAGC TCCAGCTGCC TTCTATGTCC ATGTTGACAT CCAAGACATC TACCCAGAAG 
TTCTTCTTGA GGGTACTACA GGTCATCATC CAGCTGCGGG ATGACACACG ACGAGCTAAC 
AAGAAAGCCA AGCAGACAGG CAGGCTAGGT TCCTCCGGTT TAGGCTCAGC TAGCAGCATC 
CAGGCAGCTG TTCGGCAGCT GGAGGCTGAG GCTGATGCCA TTATACAAAT GGTACGTGAG 
GGTCAAAGGG CGCGGAGACA GCAACAAGCA GCAACGTCGG AGTCTTCTAA CCAGTCAGAG 
ACATCTGTCC GGAGAGAGGA ATCGCCCATG GATGTGGACC AGCCATCTCC TAGTGCTCAA 
GATACTCAGT CCATTGTAAT CTCAGATGGA ACACCACAGG GAGAGAAGGA GAAAGAGGAG 
AAACCACCTG AGTTACCCCT GCTCAGTGAG CAACTGAGCC TAGATGAACT GTGGGACATG 
CTAGGGGAAT GTTTAAAAGA ACTGGAGGAA TCTCATGACC AGCATGCAGT ATTAGTGCTA 
CAGCCTGCTG TCGAGGCCTT CTTTTTGGTC CATGCCACAG AGCGGGAGAG CAAGCCTCCT 
GTCCGAGATA CCCGTGAGAG CCAGCTGGCA CACATCAAGG ATGAACCTCC TCCACTCTCC 
CCCGCCCCCT TAACCCCAGC CACTCCATCC TCCCTTGACC CATTCTTCTC CAGGGAGCCC 
TCATCTATGC ACATTTCCTC GAGCCTGCCC CCTGACACAC AGAAGTTCCT TCGCTTTGCA 
GAGACTCACC GCACTGTGTT AAACCAGATA CTACGGCAGT CTACCACCCA TCTTGCTGAT 
GGGCCTTTTG CTGTCTTGGT AGATTATATC CGTGTCCTCG ACTTTGATGT CAAGCGCAAA 
TATTTCCGCC AAGAGCTGGA GCGTTTGGAC GAAGGGCTCC GGAAAGAAGA TATGGCAGTG 
CATGTCCGCC GTGACCATGT ATTTGAAGAC TCCTATCGTG AGCTGCATCG CAAATCTCCT 
GAAGAAATGA AGAATCGATT GTATATAGTG TTTGAAGGAG AAGAAGGGCA GGATGCTGGG 
GGGCTCCTGC GAGAGTGGTA CATGATCATC TCTCGAGAGA TGTTCAACCC TATGTATGCC 
TTGTTCCGTA CCTCACCTGG TGACCGGGTC ACCTACACCA TCAATCCATC TTCCCACTGT 
AACCCCAACC ACCTCAGCTA CTTCAAGTTT GTTGGACGAA TTGTAGCCAA AGCTGTATAT 
GACAACCGCC TCCTGGAGTG CTACTTTACT AGGTCTTTCT ACAAACACAT CTTGGGCAAA 
TCTGTCAGGT ATACAGATAT GGAGAGTGAA GATTACCACT TCTACCAGGG CCTAGTTTAT 
CTGCTTGAAA ATGATGTCTC TACATTAGGC TATGACCTCA CCTTCAGCAC TGAGGTTCAA 
GAATTTGGAG TATGTGAAGT TCGTGACCTC AAACCCAATG GAGCCAACAT CTTGGTAACA 
GAGGAGAACA AGAAGGAATA TGTACACCTG GTTTGCCAGA TGAGAATGAC AGGAGCCATC 
CGCAAACAGC TGGCAGCCTT CTTGGAGGGC TTCTATGAAA TCATTCCAAA GCGCCTCATA 
TCCATCTTTA CTGAGCAGGA ATTAGAGCTG CTCATATCAG GGCTGCCTAC CATCGACATT 
GATGATCTAA AATCTAACAC TGAGTACCAC AAGTACCAAT CCAACTCTAT TCAGATCCAG 
TGGTTTTGGA GAGCATTGCG TTCCTTTGAC CAAGCAGACC GTGCCAAGTT CCTCCAGTTT 
GTCACAGGTA CTTCCAAGGT ACCCCTACAA GGATTTGCTG CCCTTGAAGG TATGAATGGC 
ATTCAGAAGT TTCAGATTCA TCGAGATGAC AGATCCACAG ATCGCCTGCC TTCAGCTCAC 
ACATGTTTTA ATCAACTGGA CCTGCCTGCC TATGAGAGCT TTGAGAAGCT CCGCCACATG 
CTACTATTGG CCATCCAGGA ATGCTCTGAA GGCTTTGGGC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246156.1
CDS	1191..14558
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEDKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_011247854.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247854.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGAC 
AAATCTGATC CTAAACCTGG GGGAACGACC CAAGAGGTTG GCCAGCTCCT GCAAGACATG 
GGTGACGATG TATACCAGCA GTACCGGTCA CTTACTCGTC AGAGCAGTGA CTTTGATACA 
CAGTCAGGTT TTTCCTTAAA TAGTCAGGTT TTTGCTGCAG ATGGTGCCCC TGCTGAAACT 
TCCACAACTG GAACCTCCCA AGGAGAGGGA GCTTCAACTC CAGAGGAGAC TCGAGAAGGG 
AAGAAAGATA AAGAAGGGGA CCGTACATCT GAGGAAGGCA AGCAGAAAAG CAAGGGCAGC 
AAACCTTTAA TGCCTACCTC TACTATCCTT CGTCTTCTGG CAGAGTTGGT GAGGTCCTAT 
GTTGGTATTG CTACCCTGAT TGCAAACTAC AGCTATACCG TGGGCCAGTC TGAGCTGATC 
AAAGAGGACT GCAGTGTGCT AGCTTTTGTC CTGGACCACC TCCTCCCACA CACCCAGAAT 
GCAGAAGACA AGGATACACC TGCCCTGGCA CGTCTATTCC TTGCAAGTCT GGCTGCTGCG 
GGGAGTGGCA CAGATGCTCA AGTGGCCCTA GTGAATGAAG TGAAAGCAGC CCTGGGACGG 
GCGCTTGCTA TGGCTGAGAG TACAGAGAAG CACGCCAGAC TTCAGGCAGT GATGTGTATC 
ATCAGTACTA TCATGGAATC CTGCCCCTCC ACCTCCAGCT TCTACAGTAG TGCTACAGCC 
AAGACTCAGC ATAATGGCAT GAACAACATC ATTCGACTGT TCCTAAAGAA GGGACTAGTC 
AATGACCTGG CCAGAGTTCC TCACAGCCTA GACCTGTCCA GTCCCAATAT GGCCAACACA 
GTCAATGCTG CTCTAAAACC CTTGGAAACA CTTTCCCGGA TTGTAAATCA ACCCAGTAGC 
CTTTTTGGCA GCAAGAGTGC TTCTAGCAAG AACAAGTCTG AGCAGGATGC TCAAGGAGCT 
TCTCAGGACT CCAGCAGCCA TCAGCAAGAT CCAGGCGAGC CTGGGGAGGC AGAAGTGCAG 
GAGGAGGATC ATGATGTCAC TCAGACAGAG GTGGCAGATG GGGATATCAT GGATGGGGAG 
GCTGAAACCG ACTCAGTGGT GATTGCCGGG CAGCCTGAGG TGCTCAGTTC ACAAGAGATG 
CAGGTTGAAA ATGAACTGGA GGACCTGATA GATGAGTTGC TTGAGAGGGA TGGCGGATCT 
GGGAACAGTA CAATTATAGT GAGCAGAAGT GGAGAGGATG AATCACAAGA GGACGTGCTG 
ATGGATGAAG CTCCTTCCAA CCTCAGCCAA GCTTCCACCT TGCAGGCCAA CCGAGAAGAT 
TCCATGAATA TCCTGGACCC TGAGGATGAG GAGGAACATA CTCAGGAAGA GGACAGCAGT 
GGCAGTAACG AGGATGAGGA TGATAGTCAG GATGAAGAGG AGGAGGAGGA GGAAGATGAG 
GAAGATGATC AGGAGGATGA TGAAGGTGAA GAAGGAGATG AAGATGATGA CGACGATGGT 
TCTGAGATGG AATTGGATGA GGATTACCCT GATATGAACG CTTCTCCCTT GGTCCGATTT 
GAGCGCTTTG ACCGGGAGGA TGATCTCATC ATTGAGTTTG ACAACATGTT CTCCAGTGCT 
ACAGACATTC CCCCATCCCC AGGAAATATC CCTACCACCC ACCCATTGAT GGTACGTCAT 
GCAGATCACA GTTCCCTGAC ACTGGGCAGT GGTTCTTCCA CAACTCGTCT TACCCAGGGC 
ATCGGTCGCA GTCAAAGGAC CCTCAGACAG CTCACTGCCA ATACAGGCCA TACCATTCAT 
GTTCACTACC CTGGGAATCG CCAGCCTAAC CCTCCTCTTA TACTCCAGAG GTTGCTTGGT 
CCCTCTGCAG CTGCTGACAT TCTTCAGCTG AGCAGCAGCC TTCCATTACA AAGCCGAGGC 
CGGGCCCGCC TCCTAGTGGG CAATGATGAC GTCCATATCA TCGCCAGGTC TGATGACGAG 
CTGCTGGATG ACTTTTTTCA TGACCAGAGT ACAGCCACTA GCCAAGCAGG AACCCTGTCC 
AGCATCCCAA CAGCTCTAAC CCGATGGACA GAGGAATGCA AAGTTCTTGA TGCTGAGAGT 
ATGCATGACT GTGTTTCAGT GGTTAAAGTG CCCATTGTCA ATCACCTGGA ATTCTTGAGA 
GATGAGGAGC TAGAAGAGAG AAGGGAGAAA CGCAGGAAAC AATTGGCTGA GGAAGAAACA 
AAAATAATAG ACAAAGGCAA AGAAGACAAG GAGAACAGAG ATCAGAGTGC ACAGTGTACT 
GTAACTAAGA CAAATGACTC CACTGAACAG AATGTCTCAG ATGGGACTCC TATGCCTGAC 
AGCTATCCAA CAACCCCCTC TTCAACGGAT GCACCTACAT CTGAGTCCAA GGAAACACTT 
GGTACCCTAC AACCCTCCCA GCAACAGCCA GCACTTCCAC CCCCACCATC CTTAGGAGAG 
ATTCCTCAGG AACTACAGTC CCCTGCTGAA GAAGTGGCAA ACTCTACACA GCTATTGATG 
CCTATAGAGT TGGAGGAATT GGGTCCCACA AGACCAAGTG GAGAAGCAGA AACAACTCAA 
ATGGAATTGT CTCCAGCTCC CACCATAACC TCTCTTTCCC CAGAGAGAGC TGAAGATTCA 
GACGCGTTAA CAGCTGTCAG CAGTCAGCTG GAAGGTTCTC CCATGGACAC CAGCAGCCTG 
GCTTCCTGTA CGTTAGAAGA GGCTGTGGGT GACACTCCAG CAGCTGGCAG TTCTGAGCAG 
CCCACAGCAG GTAGTTCCAC TCCTGGGGAT GCGCCATCAG TTGTGGCAGA AGTGCAAGGC 
AGACCTGATG TATCAAGAGA ATCCAACCAG CCACCTGAGG ACAGCTCCCC CCCTGCATCC 
TCGGAGAGTT CTTCCACTAG AGATTCTGCC GTGGCCATCT CTGGAGCAGA TTCTCGTGGA 
ATTCTAGAAG AGCCACTACC TTCAACAAGC AGTGAAGAAG AAGATCCCCT TGCTGGTATC 
AGTCTCCCTG AAGGTGTGGA TCCCTCCTTT CTGGCTGCTC TCCCTGATGA TATCCGTCGG 
GAAGTCCTAC AGAACCAGCT GGGCATACGT CCACCAACCC GAAGTGCTCC CTCCTCAAAT 
AGCTCAGCTC CTGCAGTAGT GGGGAACCCT GGTGTAACTG AAGTGAGCCC TGAGTTCCTG 
GCTGCCCTAC CTCCAGCTAT TCAAGAGGAA GTGCTAGCAC AGCAGAGAGC TGAACAGCAG 
CGACGGGAAC TGGCTCAGAA TGCGAGCTCA GACACCCCCA TGGACCCTGT GACTTTCATT 
CAGACTTTGC CCTCAGATCT GCGCCGTAGT GTCCTAGAGG ATATGGAAGA CAGTGTGTTA 
GCTGTGATGC CACCTGACAT TGCAGCTGAG GCTCAAGCTC TAAGACGAGA ACAAGAAGCC 
CGACAGCGGC AGCTCATGCA TGAGCGTCTT TTTGGACACA GTAGTACTTC TGCACTCTCT 
GCCATTCTCC GAAGCCCGGC TTTTACCAGT CGCCTGAGTG GCAACCGTGG GGTCCAGTAT 
ACTCGCCTTG CTGTACAGAG GGGTGGCACC TTCCAGATGG GGGGTAGCAG CAGCCATAAT 
AGGCCTTCTG GCAGTAATGT GGACACACTA CTCCGCCTCC GAGGACGACT CCTTCTAGAC 
CATGAAGCCC TGTCTTGCCT CTTGGTCCTA CTTTTTGTGG ATGAGCCAAA GCTCAATACT 
AGCCGTCTAC ACCGAGTACT GAGAAATCTC TGCTATCATG CCCAGACCCG CCACTGGGTC 
ATCCGCAGCC TCCTGTCCAT TTTGCAGCGC AGCAGTGAAA GTGAGTTATG CATTGAGACA 
CCTAAGCTCT CCACAAGTGA AGAAAGGGGC AAGAAATCAA GCAAGAGCTG TGCATCAAGC 
AGTCATGAGA ACCGTCCCCT GGATCTGCTC CACAAGATGG AGTCCAAAAG TTCCAATCAG 
CTCTCCTGGC TCTCAGTATC AATGGATGCT GCTTTAGGCT GTAGGACTAA CATATTCCAG 
ATTCAACGTT CTGGAGGGCG TAAACATACA GAGAAGCATG CAAGCAGTGG TTCCACTGTC 
CACATCCACC CCCAGGCTGC TCCTGTTGTT TGCAGACATG TTTTGGATAC ACTCATTCAG 
TTGGCCAAGG TGTTTCCCAG CCACTTTACA CAGCAGCGGA CCAAAGAAAC AAACTGTGAG 
AGTGATCGGG AACGGGGCAG TAAGCAGGCC TGCAGCCCAT GCTCCTCACA GTCCTCCAGC 
AGTGGCATCT GTACAGACTT CTGGGACTTA TTGGTAAAAC TGGACAACAT GAATGTTAGC 
CGAAAAGGCA AGAACTCCGT GAAGTCAGTA CCAGTGAGCT CTGGTGGTGA AGGGGAAACT 
TCCCCACACA GCCTTGAAGC CTCTCCACTG GGGCAGCTCA TGAACATGTT GTCACATCCA 
GTCATCCGCC GGAGTTCTCT CTTAACTGAG AAACTCCTGA GACTCCTCTC TCTCATCTCA 
ATTGCTCTCC CAGAAAACAA AGTATCAGAA GTGCAAACTA ACTCCAGCAA CAGTGGTTCC 
TCCACTGCTG CCACCTCAAA CACATCTACT ACTACCACCA CCACCACTAC TGCCACTGCC 
CCCACGCCCA CACCCCCAGC TGCAACTACC CCTGTCACTT CTGCTCCAGC TCTGGTTGCT 
GCCACAGCTA TTTCCACCAT TACTGTAGCT GCTTCTACCA CAGTGACTAC CCCCACAACT 
GCTACCACTA CTGTTTCAAC TTCTACTACT AAAGGCAGCA AATCTCCAGC AAAGGTTGGT 
GAAGGAGGCA GTGGTATAGA CTTCAAAATG GTGTCCTCTG GCCTCACTGA AAACCAGCTA 
CAGCTCTCTG TAGAGGTGTT GACATCCCAC TCTTGTTCTG AAGAAGGCTT GGAAGATGCG 
GCCAATGTGT TACTGCAGCT CTCTCGTGGG GACTCTGGTA CCCGGGACAC TGTTCTTAAG 
CTGCTACTAA ATGGAGCCCG CCATCTGGGT TATACCCTCT GTAAACAGAT AGGTACCCTA 
CTCGCTGAAC TACGGGAATA TAATCTGGAG CAGCAGCGGC GAGCCCAATG CGAGACCCTC 
TCTCCTGATG GCCTACCTGA GGAGCAACCA CAGACCACCA AACTGAAAGG CAAAATGCAG 
AGCAGGTTTG ACATGGCTGA AAATGTGGTA ATTGTGGCAT CTCAGAAGCG ACCCTTGGGT 
GGCCGGGAGC TCCAGCTGCC TTCTATGTCC ATGTTGACAT CCAAGACATC TACCCAGAAG 
TTCTTCTTGA GGGTACTACA GGTCATCATC CAGCTGCGGG ATGACACACG ACGAGCTAAC 
AAGAAAGCCA AGCAGACAGG CAGGCTAGGT TCCTCCGGTT TAGGCTCAGC TAGCAGCATC 
CAGGCAGCTG TTCGGCAGCT GGAGGCTGAG GCTGATGCCA TTATACAAAT GGTACGTGAG 
GGTCAAAGGG CGCGGAGACA GCAACAAGCA GCAACGTCGG AGTCTTCTAA CCAGTCAGAG 
ACATCTGTCC GGAGAGAGGA ATCGCCCATG GATGTGGACC AGCCATCTCC TAGTGCTCAA 
GATACTCAGT CCATTGTAAT CTCAGATGGA ACACCACAGG GAGAGAAGGA GAAAGAGGAG 
AAACCACCTG AGTTACCCCT GCTCAGTGAG CAACTGAGCC TAGATGAACT GTGGGACATG 
CTAGGGGAAT GTTTAAAAGA ACTGGAGGAA TCTCATGACC AGCATGCAGT ATTAGTGCTA 
CAGCCTGCTG TCGAGGCCTT CTTTTTGGTC CATGCCACAG AGCGGGAGAG CAAGCCTCCT 
GTCCGAGATA CCCGTGAGAG CCAGCTGGCA CACATCAAGG ATGAACCTCC TCCACTCTCC 
CCCGCCCCCT TAACCCCAGC CACTCCATCC TCCCTTGACC CATTCTTCTC CAGGGAGCCC 
TCATCTATGC ACATTTCCTC GAGCCTGCCC CCTGACACAC AGAAGTTCCT TCGCTTTGCA 
GAGACTCACC GCACTGTGTT AAACCAGATA CTACGGCAGT CTACCACCCA TCTTGCTGAT 
GGGCCTTTTG CTGTCTTGGT AGATTATATC CGTGTCCTCG ACTTTGATGT CAAGCGCAAA 
TATTTCCGCC AAGAGCTGGA GCGTTTGGAC GAAGGGCTCC GGAAAGAAGA TATGGCAGTG 
CATGTCCGCC GTGACCATGT ATTTGAAGAC TCCTATCGTG AGCTGCATCG CAAATCTCCT 
GAAGAAATGA AGAATCGATT GTATATAGTG TTTGAAGGAG AAGAAGGGCA GGATGCTGGG 
GGGCTCCTGC GAGAGTGGTA CATGATCATC TCTCGAGAGA TGTTCAACCC TATGTATGCC 
TTGTTCCGTA CCTCACCTGG TGACCGGGTC ACCTACACCA TCAATCCATC TTCCCACTGT 
AACCCCAACC ACCTCAGCTA CTTCAAGTTT GTTGGACGAA TTGTAGCCAA AGCTGTATAT 
GACAACCGCC TCCTGGAGTG CTACTTTACT AGGTCTTTCT ACAAACACAT CTTGGGCAAA 
TCTGTCAGGT ATACAGATAT GGAGAGTGAA GATTACCACT TCTACCAGGG CCTAGTTTAT 
CTGCTTGAAA ATGATGTCTC TACATTAGGC TATGACCTCA CCTTCAGCAC TGAGGTTCAA 
GAATTTGGAG TATGTGAAGT TCGTGACCTC AAACCCAATG GAGCCAACAT CTTGGTAACA 
GAGGAGAACA AGAAGGAATA TGTACACCTG GTTTGCCAGA TGAGAATGAC AGGAGCCATC 
CGCAAACAGC TGGCAGCCTT CTTGGAGGGC TTCTATGAAA TCATTCCAAA GCGCCTCATA 
TCCATCTTTA CTGAGCAGGA ATTAGAGCTG CTCATATCAG GGCTGCCTAC CATCGACATT 
GATGATCTAA AATCTAACAC TGAGTACCAC AAGTACCAAT CCAACTCTAT TCAGATCCAG 
TGGTTTTGGA GAGCATTGCG TTCCTTTGAC CAAGCAGACC GTGCCAAGTT CCTCCAGTTT 
GTCACAGGTA CTTCCAAGGT ACCCCTACAA GGATTTGCTG CCCTTGAAGG TATGAATGGC 
ATTCAGAAGT TTCAGATTCA TCGAGATGAC AGATCCACAG ATCGCCTGCC TTCAGCTCAC 
ACATGTTTTA ATCAACTGGA CCTGCCTGCC TATGAGAGCT TTGAGAAGCT CCGCCACATG 
CTACTATTGG CCATCCAGGA ATGCTCTGAA GGCTTTGGGC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251443.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107303.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X19, mRNA.

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CloneID	OMu69810
Clone ID Related Accession (Same CDS sequence)	XM_011247854.1 , XM_030251443.1
Accession Version	XM_030251443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGAC 
AAATCTGATC CTAAACCTGG GGGAACGACC CAAGAGGTTG GCCAGCTCCT GCAAGACATG 
GGTGACGATG TATACCAGCA GTACCGGTCA CTTACTCGTC AGAGCAGTGA CTTTGATACA 
CAGTCAGGTT TTTCCTTAAA TAGTCAGGTT TTTGCTGCAG ATGGTGCCCC TGCTGAAACT 
TCCACAACTG GAACCTCCCA AGGAGAGGGA GCTTCAACTC CAGAGGAGAC TCGAGAAGGG 
AAGAAAGATA AAGAAGGGGA CCGTACATCT GAGGAAGGCA AGCAGAAAAG CAAGGGCAGC 
AAACCTTTAA TGCCTACCTC TACTATCCTT CGTCTTCTGG CAGAGTTGGT GAGGTCCTAT 
GTTGGTATTG CTACCCTGAT TGCAAACTAC AGCTATACCG TGGGCCAGTC TGAGCTGATC 
AAAGAGGACT GCAGTGTGCT AGCTTTTGTC CTGGACCACC TCCTCCCACA CACCCAGAAT 
GCAGAAGACA AGGATACACC TGCCCTGGCA CGTCTATTCC TTGCAAGTCT GGCTGCTGCG 
GGGAGTGGCA CAGATGCTCA AGTGGCCCTA GTGAATGAAG TGAAAGCAGC CCTGGGACGG 
GCGCTTGCTA TGGCTGAGAG TACAGAGAAG CACGCCAGAC TTCAGGCAGT GATGTGTATC 
ATCAGTACTA TCATGGAATC CTGCCCCTCC ACCTCCAGCT TCTACAGTAG TGCTACAGCC 
AAGACTCAGC ATAATGGCAT GAACAACATC ATTCGACTGT TCCTAAAGAA GGGACTAGTC 
AATGACCTGG CCAGAGTTCC TCACAGCCTA GACCTGTCCA GTCCCAATAT GGCCAACACA 
GTCAATGCTG CTCTAAAACC CTTGGAAACA CTTTCCCGGA TTGTAAATCA ACCCAGTAGC 
CTTTTTGGCA GCAAGAGTGC TTCTAGCAAG AACAAGTCTG AGCAGGATGC TCAAGGAGCT 
TCTCAGGACT CCAGCAGCCA TCAGCAAGAT CCAGGCGAGC CTGGGGAGGC AGAAGTGCAG 
GAGGAGGATC ATGATGTCAC TCAGACAGAG GTGGCAGATG GGGATATCAT GGATGGGGAG 
GCTGAAACCG ACTCAGTGGT GATTGCCGGG CAGCCTGAGG TGCTCAGTTC ACAAGAGATG 
CAGGTTGAAA ATGAACTGGA GGACCTGATA GATGAGTTGC TTGAGAGGGA TGGCGGATCT 
GGGAACAGTA CAATTATAGT GAGCAGAAGT GGAGAGGATG AATCACAAGA GGACGTGCTG 
ATGGATGAAG CTCCTTCCAA CCTCAGCCAA GCTTCCACCT TGCAGGCCAA CCGAGAAGAT 
TCCATGAATA TCCTGGACCC TGAGGATGAG GAGGAACATA CTCAGGAAGA GGACAGCAGT 
GGCAGTAACG AGGATGAGGA TGATAGTCAG GATGAAGAGG AGGAGGAGGA GGAAGATGAG 
GAAGATGATC AGGAGGATGA TGAAGGTGAA GAAGGAGATG AAGATGATGA CGACGATGGT 
TCTGAGATGG AATTGGATGA GGATTACCCT GATATGAACG CTTCTCCCTT GGTCCGATTT 
GAGCGCTTTG ACCGGGAGGA TGATCTCATC ATTGAGTTTG ACAACATGTT CTCCAGTGCT 
ACAGACATTC CCCCATCCCC AGGAAATATC CCTACCACCC ACCCATTGAT GGTACGTCAT 
GCAGATCACA GTTCCCTGAC ACTGGGCAGT GGTTCTTCCA CAACTCGTCT TACCCAGGGC 
ATCGGTCGCA GTCAAAGGAC CCTCAGACAG CTCACTGCCA ATACAGGCCA TACCATTCAT 
GTTCACTACC CTGGGAATCG CCAGCCTAAC CCTCCTCTTA TACTCCAGAG GTTGCTTGGT 
CCCTCTGCAG CTGCTGACAT TCTTCAGCTG AGCAGCAGCC TTCCATTACA AAGCCGAGGC 
CGGGCCCGCC TCCTAGTGGG CAATGATGAC GTCCATATCA TCGCCAGGTC TGATGACGAG 
CTGCTGGATG ACTTTTTTCA TGACCAGAGT ACAGCCACTA GCCAAGCAGG AACCCTGTCC 
AGCATCCCAA CAGCTCTAAC CCGATGGACA GAGGAATGCA AAGTTCTTGA TGCTGAGAGT 
ATGCATGACT GTGTTTCAGT GGTTAAAGTG CCCATTGTCA ATCACCTGGA ATTCTTGAGA 
GATGAGGAGC TAGAAGAGAG AAGGGAGAAA CGCAGGAAAC AATTGGCTGA GGAAGAAACA 
AAAATAATAG ACAAAGGCAA AGAAGACAAG GAGAACAGAG ATCAGAGTGC ACAGTGTACT 
GTAACTAAGA CAAATGACTC CACTGAACAG AATGTCTCAG ATGGGACTCC TATGCCTGAC 
AGCTATCCAA CAACCCCCTC TTCAACGGAT GCACCTACAT CTGAGTCCAA GGAAACACTT 
GGTACCCTAC AACCCTCCCA GCAACAGCCA GCACTTCCAC CCCCACCATC CTTAGGAGAG 
ATTCCTCAGG AACTACAGTC CCCTGCTGAA GAAGTGGCAA ACTCTACACA GCTATTGATG 
CCTATAGAGT TGGAGGAATT GGGTCCCACA AGACCAAGTG GAGAAGCAGA AACAACTCAA 
ATGGAATTGT CTCCAGCTCC CACCATAACC TCTCTTTCCC CAGAGAGAGC TGAAGATTCA 
GACGCGTTAA CAGCTGTCAG CAGTCAGCTG GAAGGTTCTC CCATGGACAC CAGCAGCCTG 
GCTTCCTGTA CGTTAGAAGA GGCTGTGGGT GACACTCCAG CAGCTGGCAG TTCTGAGCAG 
CCCACAGCAG GTAGTTCCAC TCCTGGGGAT GCGCCATCAG TTGTGGCAGA AGTGCAAGGC 
AGACCTGATG TATCAAGAGA ATCCAACCAG CCACCTGAGG ACAGCTCCCC CCCTGCATCC 
TCGGAGAGTT CTTCCACTAG AGATTCTGCC GTGGCCATCT CTGGAGCAGA TTCTCGTGGA 
ATTCTAGAAG AGCCACTACC TTCAACAAGC AGTGAAGAAG AAGATCCCCT TGCTGGTATC 
AGTCTCCCTG AAGGTGTGGA TCCCTCCTTT CTGGCTGCTC TCCCTGATGA TATCCGTCGG 
GAAGTCCTAC AGAACCAGCT GGGCATACGT CCACCAACCC GAAGTGCTCC CTCCTCAAAT 
AGCTCAGCTC CTGCAGTAGT GGGGAACCCT GGTGTAACTG AAGTGAGCCC TGAGTTCCTG 
GCTGCCCTAC CTCCAGCTAT TCAAGAGGAA GTGCTAGCAC AGCAGAGAGC TGAACAGCAG 
CGACGGGAAC TGGCTCAGAA TGCGAGCTCA GACACCCCCA TGGACCCTGT GACTTTCATT 
CAGACTTTGC CCTCAGATCT GCGCCGTAGT GTCCTAGAGG ATATGGAAGA CAGTGTGTTA 
GCTGTGATGC CACCTGACAT TGCAGCTGAG GCTCAAGCTC TAAGACGAGA ACAAGAAGCC 
CGACAGCGGC AGCTCATGCA TGAGCGTCTT TTTGGACACA GTAGTACTTC TGCACTCTCT 
GCCATTCTCC GAAGCCCGGC TTTTACCAGT CGCCTGAGTG GCAACCGTGG GGTCCAGTAT 
ACTCGCCTTG CTGTACAGAG GGGTGGCACC TTCCAGATGG GGGGTAGCAG CAGCCATAAT 
AGGCCTTCTG GCAGTAATGT GGACACACTA CTCCGCCTCC GAGGACGACT CCTTCTAGAC 
CATGAAGCCC TGTCTTGCCT CTTGGTCCTA CTTTTTGTGG ATGAGCCAAA GCTCAATACT 
AGCCGTCTAC ACCGAGTACT GAGAAATCTC TGCTATCATG CCCAGACCCG CCACTGGGTC 
ATCCGCAGCC TCCTGTCCAT TTTGCAGCGC AGCAGTGAAA GTGAGTTATG CATTGAGACA 
CCTAAGCTCT CCACAAGTGA AGAAAGGGGC AAGAAATCAA GCAAGAGCTG TGCATCAAGC 
AGTCATGAGA ACCGTCCCCT GGATCTGCTC CACAAGATGG AGTCCAAAAG TTCCAATCAG 
CTCTCCTGGC TCTCAGTATC AATGGATGCT GCTTTAGGCT GTAGGACTAA CATATTCCAG 
ATTCAACGTT CTGGAGGGCG TAAACATACA GAGAAGCATG CAAGCAGTGG TTCCACTGTC 
CACATCCACC CCCAGGCTGC TCCTGTTGTT TGCAGACATG TTTTGGATAC ACTCATTCAG 
TTGGCCAAGG TGTTTCCCAG CCACTTTACA CAGCAGCGGA CCAAAGAAAC AAACTGTGAG 
AGTGATCGGG AACGGGGCAG TAAGCAGGCC TGCAGCCCAT GCTCCTCACA GTCCTCCAGC 
AGTGGCATCT GTACAGACTT CTGGGACTTA TTGGTAAAAC TGGACAACAT GAATGTTAGC 
CGAAAAGGCA AGAACTCCGT GAAGTCAGTA CCAGTGAGCT CTGGTGGTGA AGGGGAAACT 
TCCCCACACA GCCTTGAAGC CTCTCCACTG GGGCAGCTCA TGAACATGTT GTCACATCCA 
GTCATCCGCC GGAGTTCTCT CTTAACTGAG AAACTCCTGA GACTCCTCTC TCTCATCTCA 
ATTGCTCTCC CAGAAAACAA AGTATCAGAA GTGCAAACTA ACTCCAGCAA CAGTGGTTCC 
TCCACTGCTG CCACCTCAAA CACATCTACT ACTACCACCA CCACCACTAC TGCCACTGCC 
CCCACGCCCA CACCCCCAGC TGCAACTACC CCTGTCACTT CTGCTCCAGC TCTGGTTGCT 
GCCACAGCTA TTTCCACCAT TACTGTAGCT GCTTCTACCA CAGTGACTAC CCCCACAACT 
GCTACCACTA CTGTTTCAAC TTCTACTACT AAAGGCAGCA AATCTCCAGC AAAGGTTGGT 
GAAGGAGGCA GTGGTATAGA CTTCAAAATG GTGTCCTCTG GCCTCACTGA AAACCAGCTA 
CAGCTCTCTG TAGAGGTGTT GACATCCCAC TCTTGTTCTG AAGAAGGCTT GGAAGATGCG 
GCCAATGTGT TACTGCAGCT CTCTCGTGGG GACTCTGGTA CCCGGGACAC TGTTCTTAAG 
CTGCTACTAA ATGGAGCCCG CCATCTGGGT TATACCCTCT GTAAACAGAT AGGTACCCTA 
CTCGCTGAAC TACGGGAATA TAATCTGGAG CAGCAGCGGC GAGCCCAATG CGAGACCCTC 
TCTCCTGATG GCCTACCTGA GGAGCAACCA CAGACCACCA AACTGAAAGG CAAAATGCAG 
AGCAGGTTTG ACATGGCTGA AAATGTGGTA ATTGTGGCAT CTCAGAAGCG ACCCTTGGGT 
GGCCGGGAGC TCCAGCTGCC TTCTATGTCC ATGTTGACAT CCAAGACATC TACCCAGAAG 
TTCTTCTTGA GGGTACTACA GGTCATCATC CAGCTGCGGG ATGACACACG ACGAGCTAAC 
AAGAAAGCCA AGCAGACAGG CAGGCTAGGT TCCTCCGGTT TAGGCTCAGC TAGCAGCATC 
CAGGCAGCTG TTCGGCAGCT GGAGGCTGAG GCTGATGCCA TTATACAAAT GGTACGTGAG 
GGTCAAAGGG CGCGGAGACA GCAACAAGCA GCAACGTCGG AGTCTTCTAA CCAGTCAGAG 
ACATCTGTCC GGAGAGAGGA ATCGCCCATG GATGTGGACC AGCCATCTCC TAGTGCTCAA 
GATACTCAGT CCATTGTAAT CTCAGATGGA ACACCACAGG GAGAGAAGGA GAAAGAGGAG 
AAACCACCTG AGTTACCCCT GCTCAGTGAG CAACTGAGCC TAGATGAACT GTGGGACATG 
CTAGGGGAAT GTTTAAAAGA ACTGGAGGAA TCTCATGACC AGCATGCAGT ATTAGTGCTA 
CAGCCTGCTG TCGAGGCCTT CTTTTTGGTC CATGCCACAG AGCGGGAGAG CAAGCCTCCT 
GTCCGAGATA CCCGTGAGAG CCAGCTGGCA CACATCAAGG ATGAACCTCC TCCACTCTCC 
CCCGCCCCCT TAACCCCAGC CACTCCATCC TCCCTTGACC CATTCTTCTC CAGGGAGCCC 
TCATCTATGC ACATTTCCTC GAGCCTGCCC CCTGACACAC AGAAGTTCCT TCGCTTTGCA 
GAGACTCACC GCACTGTGTT AAACCAGATA CTACGGCAGT CTACCACCCA TCTTGCTGAT 
GGGCCTTTTG CTGTCTTGGT AGATTATATC CGTGTCCTCG ACTTTGATGT CAAGCGCAAA 
TATTTCCGCC AAGAGCTGGA GCGTTTGGAC GAAGGGCTCC GGAAAGAAGA TATGGCAGTG 
CATGTCCGCC GTGACCATGT ATTTGAAGAC TCCTATCGTG AGCTGCATCG CAAATCTCCT 
GAAGAAATGA AGAATCGATT GTATATAGTG TTTGAAGGAG AAGAAGGGCA GGATGCTGGG 
GGGCTCCTGC GAGAGTGGTA CATGATCATC TCTCGAGAGA TGTTCAACCC TATGTATGCC 
TTGTTCCGTA CCTCACCTGG TGACCGGGTC ACCTACACCA TCAATCCATC TTCCCACTGT 
AACCCCAACC ACCTCAGCTA CTTCAAGTTT GTTGGACGAA TTGTAGCCAA AGCTGTATAT 
GACAACCGCC TCCTGGAGTG CTACTTTACT AGGTCTTTCT ACAAACACAT CTTGGGCAAA 
TCTGTCAGGT ATACAGATAT GGAGAGTGAA GATTACCACT TCTACCAGGG CCTAGTTTAT 
CTGCTTGAAA ATGATGTCTC TACATTAGGC TATGACCTCA CCTTCAGCAC TGAGGTTCAA 
GAATTTGGAG TATGTGAAGT TCGTGACCTC AAACCCAATG GAGCCAACAT CTTGGTAACA 
GAGGAGAACA AGAAGGAATA TGTACACCTG GTTTGCCAGA TGAGAATGAC AGGAGCCATC 
CGCAAACAGC TGGCAGCCTT CTTGGAGGGC TTCTATGAAA TCATTCCAAA GCGCCTCATA 
TCCATCTTTA CTGAGCAGGA ATTAGAGCTG CTCATATCAG GGCTGCCTAC CATCGACATT 
GATGATCTAA AATCTAACAC TGAGTACCAC AAGTACCAAT CCAACTCTAT TCAGATCCAG 
TGGTTTTGGA GAGCATTGCG TTCCTTTGAC CAAGCAGACC GTGCCAAGTT CCTCCAGTTT 
GTCACAGGTA CTTCCAAGGT ACCCCTACAA GGATTTGCTG CCCTTGAAGG TATGAATGGC 
ATTCAGAAGT TTCAGATTCA TCGAGATGAC AGATCCACAG ATCGCCTGCC TTCAGCTCAC 
ACATGTTTTA ATCAACTGGA CCTGCCTGCC TATGAGAGCT TTGAGAAGCT CCGCCACATG 
CTACTATTGG CCATCCAGGA ATGCTCTGAA GGCTTTGGGC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107303.1
CDS	1856..15223
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEDKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251443.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251443.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGAC 
AAATCTGATC CTAAACCTGG GGGAACGACC CAAGAGGTTG GCCAGCTCCT GCAAGACATG 
GGTGACGATG TATACCAGCA GTACCGGTCA CTTACTCGTC AGAGCAGTGA CTTTGATACA 
CAGTCAGGTT TTTCCTTAAA TAGTCAGGTT TTTGCTGCAG ATGGTGCCCC TGCTGAAACT 
TCCACAACTG GAACCTCCCA AGGAGAGGGA GCTTCAACTC CAGAGGAGAC TCGAGAAGGG 
AAGAAAGATA AAGAAGGGGA CCGTACATCT GAGGAAGGCA AGCAGAAAAG CAAGGGCAGC 
AAACCTTTAA TGCCTACCTC TACTATCCTT CGTCTTCTGG CAGAGTTGGT GAGGTCCTAT 
GTTGGTATTG CTACCCTGAT TGCAAACTAC AGCTATACCG TGGGCCAGTC TGAGCTGATC 
AAAGAGGACT GCAGTGTGCT AGCTTTTGTC CTGGACCACC TCCTCCCACA CACCCAGAAT 
GCAGAAGACA AGGATACACC TGCCCTGGCA CGTCTATTCC TTGCAAGTCT GGCTGCTGCG 
GGGAGTGGCA CAGATGCTCA AGTGGCCCTA GTGAATGAAG TGAAAGCAGC CCTGGGACGG 
GCGCTTGCTA TGGCTGAGAG TACAGAGAAG CACGCCAGAC TTCAGGCAGT GATGTGTATC 
ATCAGTACTA TCATGGAATC CTGCCCCTCC ACCTCCAGCT TCTACAGTAG TGCTACAGCC 
AAGACTCAGC ATAATGGCAT GAACAACATC ATTCGACTGT TCCTAAAGAA GGGACTAGTC 
AATGACCTGG CCAGAGTTCC TCACAGCCTA GACCTGTCCA GTCCCAATAT GGCCAACACA 
GTCAATGCTG CTCTAAAACC CTTGGAAACA CTTTCCCGGA TTGTAAATCA ACCCAGTAGC 
CTTTTTGGCA GCAAGAGTGC TTCTAGCAAG AACAAGTCTG AGCAGGATGC TCAAGGAGCT 
TCTCAGGACT CCAGCAGCCA TCAGCAAGAT CCAGGCGAGC CTGGGGAGGC AGAAGTGCAG 
GAGGAGGATC ATGATGTCAC TCAGACAGAG GTGGCAGATG GGGATATCAT GGATGGGGAG 
GCTGAAACCG ACTCAGTGGT GATTGCCGGG CAGCCTGAGG TGCTCAGTTC ACAAGAGATG 
CAGGTTGAAA ATGAACTGGA GGACCTGATA GATGAGTTGC TTGAGAGGGA TGGCGGATCT 
GGGAACAGTA CAATTATAGT GAGCAGAAGT GGAGAGGATG AATCACAAGA GGACGTGCTG 
ATGGATGAAG CTCCTTCCAA CCTCAGCCAA GCTTCCACCT TGCAGGCCAA CCGAGAAGAT 
TCCATGAATA TCCTGGACCC TGAGGATGAG GAGGAACATA CTCAGGAAGA GGACAGCAGT 
GGCAGTAACG AGGATGAGGA TGATAGTCAG GATGAAGAGG AGGAGGAGGA GGAAGATGAG 
GAAGATGATC AGGAGGATGA TGAAGGTGAA GAAGGAGATG AAGATGATGA CGACGATGGT 
TCTGAGATGG AATTGGATGA GGATTACCCT GATATGAACG CTTCTCCCTT GGTCCGATTT 
GAGCGCTTTG ACCGGGAGGA TGATCTCATC ATTGAGTTTG ACAACATGTT CTCCAGTGCT 
ACAGACATTC CCCCATCCCC AGGAAATATC CCTACCACCC ACCCATTGAT GGTACGTCAT 
GCAGATCACA GTTCCCTGAC ACTGGGCAGT GGTTCTTCCA CAACTCGTCT TACCCAGGGC 
ATCGGTCGCA GTCAAAGGAC CCTCAGACAG CTCACTGCCA ATACAGGCCA TACCATTCAT 
GTTCACTACC CTGGGAATCG CCAGCCTAAC CCTCCTCTTA TACTCCAGAG GTTGCTTGGT 
CCCTCTGCAG CTGCTGACAT TCTTCAGCTG AGCAGCAGCC TTCCATTACA AAGCCGAGGC 
CGGGCCCGCC TCCTAGTGGG CAATGATGAC GTCCATATCA TCGCCAGGTC TGATGACGAG 
CTGCTGGATG ACTTTTTTCA TGACCAGAGT ACAGCCACTA GCCAAGCAGG AACCCTGTCC 
AGCATCCCAA CAGCTCTAAC CCGATGGACA GAGGAATGCA AAGTTCTTGA TGCTGAGAGT 
ATGCATGACT GTGTTTCAGT GGTTAAAGTG CCCATTGTCA ATCACCTGGA ATTCTTGAGA 
GATGAGGAGC TAGAAGAGAG AAGGGAGAAA CGCAGGAAAC AATTGGCTGA GGAAGAAACA 
AAAATAATAG ACAAAGGCAA AGAAGACAAG GAGAACAGAG ATCAGAGTGC ACAGTGTACT 
GTAACTAAGA CAAATGACTC CACTGAACAG AATGTCTCAG ATGGGACTCC TATGCCTGAC 
AGCTATCCAA CAACCCCCTC TTCAACGGAT GCACCTACAT CTGAGTCCAA GGAAACACTT 
GGTACCCTAC AACCCTCCCA GCAACAGCCA GCACTTCCAC CCCCACCATC CTTAGGAGAG 
ATTCCTCAGG AACTACAGTC CCCTGCTGAA GAAGTGGCAA ACTCTACACA GCTATTGATG 
CCTATAGAGT TGGAGGAATT GGGTCCCACA AGACCAAGTG GAGAAGCAGA AACAACTCAA 
ATGGAATTGT CTCCAGCTCC CACCATAACC TCTCTTTCCC CAGAGAGAGC TGAAGATTCA 
GACGCGTTAA CAGCTGTCAG CAGTCAGCTG GAAGGTTCTC CCATGGACAC CAGCAGCCTG 
GCTTCCTGTA CGTTAGAAGA GGCTGTGGGT GACACTCCAG CAGCTGGCAG TTCTGAGCAG 
CCCACAGCAG GTAGTTCCAC TCCTGGGGAT GCGCCATCAG TTGTGGCAGA AGTGCAAGGC 
AGACCTGATG TATCAAGAGA ATCCAACCAG CCACCTGAGG ACAGCTCCCC CCCTGCATCC 
TCGGAGAGTT CTTCCACTAG AGATTCTGCC GTGGCCATCT CTGGAGCAGA TTCTCGTGGA 
ATTCTAGAAG AGCCACTACC TTCAACAAGC AGTGAAGAAG AAGATCCCCT TGCTGGTATC 
AGTCTCCCTG AAGGTGTGGA TCCCTCCTTT CTGGCTGCTC TCCCTGATGA TATCCGTCGG 
GAAGTCCTAC AGAACCAGCT GGGCATACGT CCACCAACCC GAAGTGCTCC CTCCTCAAAT 
AGCTCAGCTC CTGCAGTAGT GGGGAACCCT GGTGTAACTG AAGTGAGCCC TGAGTTCCTG 
GCTGCCCTAC CTCCAGCTAT TCAAGAGGAA GTGCTAGCAC AGCAGAGAGC TGAACAGCAG 
CGACGGGAAC TGGCTCAGAA TGCGAGCTCA GACACCCCCA TGGACCCTGT GACTTTCATT 
CAGACTTTGC CCTCAGATCT GCGCCGTAGT GTCCTAGAGG ATATGGAAGA CAGTGTGTTA 
GCTGTGATGC CACCTGACAT TGCAGCTGAG GCTCAAGCTC TAAGACGAGA ACAAGAAGCC 
CGACAGCGGC AGCTCATGCA TGAGCGTCTT TTTGGACACA GTAGTACTTC TGCACTCTCT 
GCCATTCTCC GAAGCCCGGC TTTTACCAGT CGCCTGAGTG GCAACCGTGG GGTCCAGTAT 
ACTCGCCTTG CTGTACAGAG GGGTGGCACC TTCCAGATGG GGGGTAGCAG CAGCCATAAT 
AGGCCTTCTG GCAGTAATGT GGACACACTA CTCCGCCTCC GAGGACGACT CCTTCTAGAC 
CATGAAGCCC TGTCTTGCCT CTTGGTCCTA CTTTTTGTGG ATGAGCCAAA GCTCAATACT 
AGCCGTCTAC ACCGAGTACT GAGAAATCTC TGCTATCATG CCCAGACCCG CCACTGGGTC 
ATCCGCAGCC TCCTGTCCAT TTTGCAGCGC AGCAGTGAAA GTGAGTTATG CATTGAGACA 
CCTAAGCTCT CCACAAGTGA AGAAAGGGGC AAGAAATCAA GCAAGAGCTG TGCATCAAGC 
AGTCATGAGA ACCGTCCCCT GGATCTGCTC CACAAGATGG AGTCCAAAAG TTCCAATCAG 
CTCTCCTGGC TCTCAGTATC AATGGATGCT GCTTTAGGCT GTAGGACTAA CATATTCCAG 
ATTCAACGTT CTGGAGGGCG TAAACATACA GAGAAGCATG CAAGCAGTGG TTCCACTGTC 
CACATCCACC CCCAGGCTGC TCCTGTTGTT TGCAGACATG TTTTGGATAC ACTCATTCAG 
TTGGCCAAGG TGTTTCCCAG CCACTTTACA CAGCAGCGGA CCAAAGAAAC AAACTGTGAG 
AGTGATCGGG AACGGGGCAG TAAGCAGGCC TGCAGCCCAT GCTCCTCACA GTCCTCCAGC 
AGTGGCATCT GTACAGACTT CTGGGACTTA TTGGTAAAAC TGGACAACAT GAATGTTAGC 
CGAAAAGGCA AGAACTCCGT GAAGTCAGTA CCAGTGAGCT CTGGTGGTGA AGGGGAAACT 
TCCCCACACA GCCTTGAAGC CTCTCCACTG GGGCAGCTCA TGAACATGTT GTCACATCCA 
GTCATCCGCC GGAGTTCTCT CTTAACTGAG AAACTCCTGA GACTCCTCTC TCTCATCTCA 
ATTGCTCTCC CAGAAAACAA AGTATCAGAA GTGCAAACTA ACTCCAGCAA CAGTGGTTCC 
TCCACTGCTG CCACCTCAAA CACATCTACT ACTACCACCA CCACCACTAC TGCCACTGCC 
CCCACGCCCA CACCCCCAGC TGCAACTACC CCTGTCACTT CTGCTCCAGC TCTGGTTGCT 
GCCACAGCTA TTTCCACCAT TACTGTAGCT GCTTCTACCA CAGTGACTAC CCCCACAACT 
GCTACCACTA CTGTTTCAAC TTCTACTACT AAAGGCAGCA AATCTCCAGC AAAGGTTGGT 
GAAGGAGGCA GTGGTATAGA CTTCAAAATG GTGTCCTCTG GCCTCACTGA AAACCAGCTA 
CAGCTCTCTG TAGAGGTGTT GACATCCCAC TCTTGTTCTG AAGAAGGCTT GGAAGATGCG 
GCCAATGTGT TACTGCAGCT CTCTCGTGGG GACTCTGGTA CCCGGGACAC TGTTCTTAAG 
CTGCTACTAA ATGGAGCCCG CCATCTGGGT TATACCCTCT GTAAACAGAT AGGTACCCTA 
CTCGCTGAAC TACGGGAATA TAATCTGGAG CAGCAGCGGC GAGCCCAATG CGAGACCCTC 
TCTCCTGATG GCCTACCTGA GGAGCAACCA CAGACCACCA AACTGAAAGG CAAAATGCAG 
AGCAGGTTTG ACATGGCTGA AAATGTGGTA ATTGTGGCAT CTCAGAAGCG ACCCTTGGGT 
GGCCGGGAGC TCCAGCTGCC TTCTATGTCC ATGTTGACAT CCAAGACATC TACCCAGAAG 
TTCTTCTTGA GGGTACTACA GGTCATCATC CAGCTGCGGG ATGACACACG ACGAGCTAAC 
AAGAAAGCCA AGCAGACAGG CAGGCTAGGT TCCTCCGGTT TAGGCTCAGC TAGCAGCATC 
CAGGCAGCTG TTCGGCAGCT GGAGGCTGAG GCTGATGCCA TTATACAAAT GGTACGTGAG 
GGTCAAAGGG CGCGGAGACA GCAACAAGCA GCAACGTCGG AGTCTTCTAA CCAGTCAGAG 
ACATCTGTCC GGAGAGAGGA ATCGCCCATG GATGTGGACC AGCCATCTCC TAGTGCTCAA 
GATACTCAGT CCATTGTAAT CTCAGATGGA ACACCACAGG GAGAGAAGGA GAAAGAGGAG 
AAACCACCTG AGTTACCCCT GCTCAGTGAG CAACTGAGCC TAGATGAACT GTGGGACATG 
CTAGGGGAAT GTTTAAAAGA ACTGGAGGAA TCTCATGACC AGCATGCAGT ATTAGTGCTA 
CAGCCTGCTG TCGAGGCCTT CTTTTTGGTC CATGCCACAG AGCGGGAGAG CAAGCCTCCT 
GTCCGAGATA CCCGTGAGAG CCAGCTGGCA CACATCAAGG ATGAACCTCC TCCACTCTCC 
CCCGCCCCCT TAACCCCAGC CACTCCATCC TCCCTTGACC CATTCTTCTC CAGGGAGCCC 
TCATCTATGC ACATTTCCTC GAGCCTGCCC CCTGACACAC AGAAGTTCCT TCGCTTTGCA 
GAGACTCACC GCACTGTGTT AAACCAGATA CTACGGCAGT CTACCACCCA TCTTGCTGAT 
GGGCCTTTTG CTGTCTTGGT AGATTATATC CGTGTCCTCG ACTTTGATGT CAAGCGCAAA 
TATTTCCGCC AAGAGCTGGA GCGTTTGGAC GAAGGGCTCC GGAAAGAAGA TATGGCAGTG 
CATGTCCGCC GTGACCATGT ATTTGAAGAC TCCTATCGTG AGCTGCATCG CAAATCTCCT 
GAAGAAATGA AGAATCGATT GTATATAGTG TTTGAAGGAG AAGAAGGGCA GGATGCTGGG 
GGGCTCCTGC GAGAGTGGTA CATGATCATC TCTCGAGAGA TGTTCAACCC TATGTATGCC 
TTGTTCCGTA CCTCACCTGG TGACCGGGTC ACCTACACCA TCAATCCATC TTCCCACTGT 
AACCCCAACC ACCTCAGCTA CTTCAAGTTT GTTGGACGAA TTGTAGCCAA AGCTGTATAT 
GACAACCGCC TCCTGGAGTG CTACTTTACT AGGTCTTTCT ACAAACACAT CTTGGGCAAA 
TCTGTCAGGT ATACAGATAT GGAGAGTGAA GATTACCACT TCTACCAGGG CCTAGTTTAT 
CTGCTTGAAA ATGATGTCTC TACATTAGGC TATGACCTCA CCTTCAGCAC TGAGGTTCAA 
GAATTTGGAG TATGTGAAGT TCGTGACCTC AAACCCAATG GAGCCAACAT CTTGGTAACA 
GAGGAGAACA AGAAGGAATA TGTACACCTG GTTTGCCAGA TGAGAATGAC AGGAGCCATC 
CGCAAACAGC TGGCAGCCTT CTTGGAGGGC TTCTATGAAA TCATTCCAAA GCGCCTCATA 
TCCATCTTTA CTGAGCAGGA ATTAGAGCTG CTCATATCAG GGCTGCCTAC CATCGACATT 
GATGATCTAA AATCTAACAC TGAGTACCAC AAGTACCAAT CCAACTCTAT TCAGATCCAG 
TGGTTTTGGA GAGCATTGCG TTCCTTTGAC CAAGCAGACC GTGCCAAGTT CCTCCAGTTT 
GTCACAGGTA CTTCCAAGGT ACCCCTACAA GGATTTGCTG CCCTTGAAGG TATGAATGGC 
ATTCAGAAGT TTCAGATTCA TCGAGATGAC AGATCCACAG ATCGCCTGCC TTCAGCTCAC 
ACATGTTTTA ATCAACTGGA CCTGCCTGCC TATGAGAGCT TTGAGAAGCT CCGCCACATG 
CTACTATTGG CCATCCAGGA ATGCTCTGAA GGCTTTGGGC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_011247855.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246157.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X14, mRNA.

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CloneID	OMu69811
Clone ID Related Accession (Same CDS sequence)	XM_011247855.1 , XM_030251444.1
Accession Version	XM_011247855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG ATGATGAATT TGAGAATCTT AGAATTAAAG GTCCTAATGC TGTACAGCTG 
GTAAAGACAA CACCTTTGAA GCCCTCATCT CTGCCTGTCA TTCCTGATAC TATTAAGGAA 
GTTATCTATG ATATGCTGAA TGCTTTGGCT GCATACCATG CTCCAGAGGA AGCAGACAAA 
TCTGATCCTA AACCTGGGGG AACGACCCAA GAGGTTGGCC AGCTCCTGCA AGACATGGGT 
GACGATGTAT ACCAGCAGTA CCGGTCACTT ACTCGTCAGA GCAGTGACTT TGATACACAG 
TCAGGTTTTT CCTTAAATAG TCAGGTTTTT GCTGCAGATG GTGCCCCTGC TGAAACTTCC 
ACAACTGGAA CCTCCCAAGG AGAGGGAGCT TCAACTCCAG AGGAGACTCG AGAAGGGAAG 
AAAGATAAAG AAGGGGACCG TACATCTGAG GAAGGCAAGC AGAAAAGCAA GGGCAGCAAA 
CCTTTAATGC CTACCTCTAC TATCCTTCGT CTTCTGGCAG AGTTGGTGAG GTCCTATGTT 
GGTATTGCTA CCCTGATTGC AAACTACAGC TATACCGTGG GCCAGTCTGA GCTGATCAAA 
GAGGACTGCA GTGTGCTAGC TTTTGTCCTG GACCACCTCC TCCCACACAC CCAGAATGCA 
GAAGACAAGG ATACACCTGC CCTGGCACGT CTATTCCTTG CAAGTCTGGC TGCTGCGGGG 
AGTGGCACAG ATGCTCAAGT GGCCCTAGTG AATGAAGTGA AAGCAGCCCT GGGACGGGCG 
CTTGCTATGG CTGAGAGTAC AGAGAAGCAC GCCAGACTTC AGGCAGTGAT GTGTATCATC 
AGTACTATCA TGGAATCCTG CCCCTCCACC TCCAGCTTCT ACAGTAGTGC TACAGCCAAG 
ACTCAGCATA ATGGCATGAA CAACATCATT CGACTGTTCC TAAAGAAGGG ACTAGTCAAT 
GACCTGGCCA GAGTTCCTCA CAGCCTAGAC CTGTCCAGTC CCAATATGGC CAACACAGTC 
AATGCTGCTC TAAAACCCTT GGAAACACTT TCCCGGATTG TAAATCAACC CAGTAGCCTT 
TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC AAGTCTGAGC AGGATGCTCA AGGAGCTTCT 
CAGGACTCCA GCAGCCATCA GCAAGATCCA GGCGAGCCTG GGGAGGCAGA AGTGCAGGAG 
GAGGATCATG ATGTCACTCA GACAGAGGTG GCAGATGGGG ATATCATGGA TGGGGAGGCT 
GAAACCGACT CAGTGGTGAT TGCCGGGCAG CCTGAGGTGC TCAGTTCACA AGAGATGCAG 
GTTGAAAATG AACTGGAGGA CCTGATAGAT GAGTTGCTTG AGAGGGATGG CGGATCTGGG 
AACAGTACAA TTATAGTGAG CAGAAGTGGA GAGGATGAAT CACAAGAGGA CGTGCTGATG 
GATGAAGCTC CTTCCAACCT CAGCCAAGCT TCCACCTTGC AGGCCAACCG AGAAGATTCC 
ATGAATATCC TGGACCCTGA GGATGAGGAG GAACATACTC AGGAAGAGGA CAGCAGTGGC 
AGTAACGAGG ATGAGGATGA TAGTCAGGAT GAAGAGGAGG AGGAGGAGGA AGATGAGGAA 
GATGATCAGG AGGATGATGA AGGTGAAGAA GGAGATGAAG ATGATGACGA CGATGGTTCT 
GAGATGGAAT TGGATGAGGA TTACCCTGAT ATGAACGCTT CTCCCTTGGT CCGATTTGAG 
CGCTTTGACC GGGAGGATGA TCTCATCATT GAGTTTGACA ACATGTTCTC CAGTGCTACA 
GACATTCCCC CATCCCCAGG AAATATCCCT ACCACCCACC CATTGATGGT ACGTCATGCA 
GATCACAGTT CCCTGACACT GGGCAGTGGT TCTTCCACAA CTCGTCTTAC CCAGGGCATC 
GGTCGCAGTC AAAGGACCCT CAGACAGCTC ACTGCCAATA CAGGCCATAC CATTCATGTT 
CACTACCCTG GGAATCGCCA GCCTAACCCT CCTCTTATAC TCCAGAGGTT GCTTGGTCCC 
TCTGCAGCTG CTGACATTCT TCAGCTGAGC AGCAGCCTTC CATTACAAAG CCGAGGCCGG 
GCCCGCCTCC TAGTGGGCAA TGATGACGTC CATATCATCG CCAGGTCTGA TGACGAGCTG 
CTGGATGACT TTTTTCATGA CCAGAGTACA GCCACTAGCC AAGCAGGAAC CCTGTCCAGC 
ATCCCAACAG CTCTAACCCG ATGGACAGAG GAATGCAAAG TTCTTGATGC TGAGAGTATG 
CATGACTGTG TTTCAGTGGT TAAAGTGCCC ATTGTCAATC ACCTGGAATT CTTGAGAGAT 
GAGGAGCTAG AAGAGAGAAG GGAGAAACGC AGGAAACAAT TGGCTGAGGA AGAAACAAAA 
ATAATAGACA AAGGCAAAGA AGACAAGGAG AACAGAGATC AGAGTGCACA GTGTACTGTA 
ACTAAGACAA ATGACTCCAC TGAACAGAAT GTCTCAGATG GGACTCCTAT GCCTGACAGC 
TATCCAACAA CCCCCTCTTC AACGGATGCA CCTACATCTG AGTCCAAGGA AACACTTGGT 
ACCCTACAAC CCTCCCAGCA ACAGCCAGCA CTTCCACCCC CACCATCCTT AGGAGAGATT 
CCTCAGGAAC TACAGTCCCC TGCTGAAGAA GTGGCAAACT CTACACAGCT ATTGATGCCT 
ATAGAGTTGG AGGAATTGGG TCCCACAAGA CCAAGTGGAG AAGCAGAAAC AACTCAAATG 
GAATTGTCTC CAGCTCCCAC CATAACCTCT CTTTCCCCAG AGAGAGCTGA AGATTCAGAC 
GCGTTAACAG CTGTCAGCAG TCAGCTGGAA GGTTCTCCCA TGGACACCAG CAGCCTGGCT 
TCCTGTACGT TAGAAGAGGC TGTGGGTGAC ACTCCAGCAG CTGGCAGTTC TGAGCAGCCC 
ACAGCAGGTA GTTCCACTCC TGGGGATGCG CCATCAGTTG TGGCAGAAGT GCAAGGCAGA 
CCTGATGTAT CAAGAGAATC CAACCAGCCA CCTGAGGACA GCTCCCCCCC TGCATCCTCG 
GAGAGTTCTT CCACTAGAGA TTCTGCCGTG GCCATCTCTG GAGCAGATTC TCGTGGAATT 
CTAGAAGAGC CACTACCTTC AACAAGCAGT GAAGAAGAAG ATCCCCTTGC TGGTATCAGT 
CTCCCTGAAG GTGTGGATCC CTCCTTTCTG GCTGCTCTCC CTGATGATAT CCGTCGGGAA 
GTCCTACAGA ACCAGCTGGG CATACGTCCA CCAACCCGAA GTGCTCCCTC CTCAAATAGC 
TCAGCTCCTG CAGTAGTGGG GAACCCTGGT GTAACTGAAG TGAGCCCTGA GTTCCTGGCT 
GCCCTACCTC CAGCTATTCA AGAGGAAGTG CTAGCACAGC AGAGAGCTGA ACAGCAGCGA 
CGGGAACTGG CTCAGAATGC GAGCTCAGAC ACCCCCATGG ACCCTGTGAC TTTCATTCAG 
ACTTTGCCCT CAGATCTGCG CCGTAGTGTC CTAGAGGATA TGGAAGACAG TGTGTTAGCT 
GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCTCTAA GACGAGAACA AGAAGCCCGA 
CAGCGGCAGC TCATGCATGA GCGTCTTTTT GGACACAGTA GTACTTCTGC ACTCTCTGCC 
ATTCTCCGAA GCCCGGCTTT TACCAGTCGC CTGAGTGGCA ACCGTGGGGT CCAGTATACT 
CGCCTTGCTG TACAGAGGGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAATAGG 
CCTTCTGGCA GTAATGTGGA CACACTACTC CGCCTCCGAG GACGACTCCT TCTAGACCAT 
GAAGCCCTGT CTTGCCTCTT GGTCCTACTT TTTGTGGATG AGCCAAAGCT CAATACTAGC 
CGTCTACACC GAGTACTGAG AAATCTCTGC TATCATGCCC AGACCCGCCA CTGGGTCATC 
CGCAGCCTCC TGTCCATTTT GCAGCGCAGC AGTGAAAGTG AGTTATGCAT TGAGACACCT 
AAGCTCTCCA CAAGTGAAGA AAGGGGCAAG AAATCAAGCA AGAGCTGTGC ATCAAGCAGT 
CATGAGAACC GTCCCCTGGA TCTGCTCCAC AAGATGGAGT CCAAAAGTTC CAATCAGCTC 
TCCTGGCTCT CAGTATCAAT GGATGCTGCT TTAGGCTGTA GGACTAACAT ATTCCAGATT 
CAACGTTCTG GAGGGCGTAA ACATACAGAG AAGCATGCAA GCAGTGGTTC CACTGTCCAC 
ATCCACCCCC AGGCTGCTCC TGTTGTTTGC AGACATGTTT TGGATACACT CATTCAGTTG 
GCCAAGGTGT TTCCCAGCCA CTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT 
GATCGGGAAC GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CCTCACAGTC CTCCAGCAGT 
GGCATCTGTA CAGACTTCTG GGACTTATTG GTAAAACTGG ACAACATGAA TGTTAGCCGA 
AAAGGCAAGA ACTCCGTGAA GTCAGTACCA GTGAGCTCTG GTGGTGAAGG GGAAACTTCC 
CCACACAGCC TTGAAGCCTC TCCACTGGGG CAGCTCATGA ACATGTTGTC ACATCCAGTC 
ATCCGCCGGA GTTCTCTCTT AACTGAGAAA CTCCTGAGAC TCCTCTCTCT CATCTCAATT 
GCTCTCCCAG AAAACAAAGT ATCAGAAGTG CAAACTAACT CCAGCAACAG TGGTTCCTCC 
ACTGCTGCCA CCTCAAACAC ATCTACTACT ACCACCACCA CCACTACTGC CACTGCCCCC 
ACGCCCACAC CCCCAGCTGC AACTACCCCT GTCACTTCTG CTCCAGCTCT GGTTGCTGCC 
ACAGCTATTT CCACCATTAC TGTAGCTGCT TCTACCACAG TGACTACCCC CACAACTGCT 
ACCACTACTG TTTCAACTTC TACTACTAAA GGCAGCAAAT CTCCAGCAAA GGTTGGTGAA 
GGAGGCAGTG GTATAGACTT CAAAATGGTG TCCTCTGGCC TCACTGAAAA CCAGCTACAG 
CTCTCTGTAG AGGTGTTGAC ATCCCACTCT TGTTCTGAAG AAGGCTTGGA AGATGCGGCC 
AATGTGTTAC TGCAGCTCTC TCGTGGGGAC TCTGGTACCC GGGACACTGT TCTTAAGCTG 
CTACTAAATG GAGCCCGCCA TCTGGGTTAT ACCCTCTGTA AACAGATAGG TACCCTACTC 
GCTGAACTAC GGGAATATAA TCTGGAGCAG CAGCGGCGAG CCCAATGCGA GACCCTCTCT 
CCTGATGGCC TACCTGAGGA GCAACCACAG ACCACCAAAC TGAAAGGCAA AATGCAGAGC 
AGGTTTGACA TGGCTGAAAA TGTGGTAATT GTGGCATCTC AGAAGCGACC CTTGGGTGGC 
CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACATCTAC CCAGAAGTTC 
TTCTTGAGGG TACTACAGGT CATCATCCAG CTGCGGGATG ACACACGACG AGCTAACAAG 
AAAGCCAAGC AGACAGGCAG GCTAGGTTCC TCCGGTTTAG GCTCAGCTAG CAGCATCCAG 
GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT 
CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CTTCTAACCA GTCAGAGACA 
TCTGTCCGGA GAGAGGAATC GCCCATGGAT GTGGACCAGC CATCTCCTAG TGCTCAAGAT 
ACTCAGTCCA TTGTAATCTC AGATGGAACA CCACAGGGAG AGAAGGAGAA AGAGGAGAAA 
CCACCTGAGT TACCCCTGCT CAGTGAGCAA CTGAGCCTAG ATGAACTGTG GGACATGCTA 
GGGGAATGTT TAAAAGAACT GGAGGAATCT CATGACCAGC ATGCAGTATT AGTGCTACAG 
CCTGCTGTCG AGGCCTTCTT TTTGGTCCAT GCCACAGAGC GGGAGAGCAA GCCTCCTGTC 
CGAGATACCC GTGAGAGCCA GCTGGCACAC ATCAAGGATG AACCTCCTCC ACTCTCCCCC 
GCCCCCTTAA CCCCAGCCAC TCCATCCTCC CTTGACCCAT TCTTCTCCAG GGAGCCCTCA 
TCTATGCACA TTTCCTCGAG CCTGCCCCCT GACACACAGA AGTTCCTTCG CTTTGCAGAG 
ACTCACCGCA CTGTGTTAAA CCAGATACTA CGGCAGTCTA CCACCCATCT TGCTGATGGG 
CCTTTTGCTG TCTTGGTAGA TTATATCCGT GTCCTCGACT TTGATGTCAA GCGCAAATAT 
TTCCGCCAAG AGCTGGAGCG TTTGGACGAA GGGCTCCGGA AAGAAGATAT GGCAGTGCAT 
GTCCGCCGTG ACCATGTATT TGAAGACTCC TATCGTGAGC TGCATCGCAA ATCTCCTGAA 
GAAATGAAGA ATCGATTGTA TATAGTGTTT GAAGGAGAAG AAGGGCAGGA TGCTGGGGGG 
CTCCTGCGAG AGTGGTACAT GATCATCTCT CGAGAGATGT TCAACCCTAT GTATGCCTTG 
TTCCGTACCT CACCTGGTGA CCGGGTCACC TACACCATCA ATCCATCTTC CCACTGTAAC 
CCCAACCACC TCAGCTACTT CAAGTTTGTT GGACGAATTG TAGCCAAAGC TGTATATGAC 
AACCGCCTCC TGGAGTGCTA CTTTACTAGG TCTTTCTACA AACACATCTT GGGCAAATCT 
GTCAGGTATA CAGATATGGA GAGTGAAGAT TACCACTTCT ACCAGGGCCT AGTTTATCTG 
CTTGAAAATG ATGTCTCTAC ATTAGGCTAT GACCTCACCT TCAGCACTGA GGTTCAAGAA 
TTTGGAGTAT GTGAAGTTCG TGACCTCAAA CCCAATGGAG CCAACATCTT GGTAACAGAG 
GAGAACAAGA AGGAATATGT ACACCTGGTT TGCCAGATGA GAATGACAGG AGCCATCCGC 
AAACAGCTGG CAGCCTTCTT GGAGGGCTTC TATGAAATCA TTCCAAAGCG CCTCATATCC 
ATCTTTACTG AGCAGGAATT AGAGCTGCTC ATATCAGGGC TGCCTACCAT CGACATTGAT 
GATCTAAAAT CTAACACTGA GTACCACAAG TACCAATCCA ACTCTATTCA GATCCAGTGG 
TTTTGGAGAG CATTGCGTTC CTTTGACCAA GCAGACCGTG CCAAGTTCCT CCAGTTTGTC 
ACAGGTACTT CCAAGGTACC CCTACAAGGA TTTGCTGCCC TTGAAGGTAT GAATGGCATT 
CAGAAGTTTC AGATTCATCG AGATGACAGA TCCACAGATC GCCTGCCTTC AGCTCACACA 
TGTTTTAATC AACTGGACCT GCCTGCCTAT GAGAGCTTTG AGAAGCTCCG CCACATGCTA 
CTATTGGCCA TCCAGGAATG CTCTGAAGGC TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246157.1
CDS	1191..14555
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_011247855.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247855.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG ATGATGAATT TGAGAATCTT AGAATTAAAG GTCCTAATGC TGTACAGCTG 
GTAAAGACAA CACCTTTGAA GCCCTCATCT CTGCCTGTCA TTCCTGATAC TATTAAGGAA 
GTTATCTATG ATATGCTGAA TGCTTTGGCT GCATACCATG CTCCAGAGGA AGCAGACAAA 
TCTGATCCTA AACCTGGGGG AACGACCCAA GAGGTTGGCC AGCTCCTGCA AGACATGGGT 
GACGATGTAT ACCAGCAGTA CCGGTCACTT ACTCGTCAGA GCAGTGACTT TGATACACAG 
TCAGGTTTTT CCTTAAATAG TCAGGTTTTT GCTGCAGATG GTGCCCCTGC TGAAACTTCC 
ACAACTGGAA CCTCCCAAGG AGAGGGAGCT TCAACTCCAG AGGAGACTCG AGAAGGGAAG 
AAAGATAAAG AAGGGGACCG TACATCTGAG GAAGGCAAGC AGAAAAGCAA GGGCAGCAAA 
CCTTTAATGC CTACCTCTAC TATCCTTCGT CTTCTGGCAG AGTTGGTGAG GTCCTATGTT 
GGTATTGCTA CCCTGATTGC AAACTACAGC TATACCGTGG GCCAGTCTGA GCTGATCAAA 
GAGGACTGCA GTGTGCTAGC TTTTGTCCTG GACCACCTCC TCCCACACAC CCAGAATGCA 
GAAGACAAGG ATACACCTGC CCTGGCACGT CTATTCCTTG CAAGTCTGGC TGCTGCGGGG 
AGTGGCACAG ATGCTCAAGT GGCCCTAGTG AATGAAGTGA AAGCAGCCCT GGGACGGGCG 
CTTGCTATGG CTGAGAGTAC AGAGAAGCAC GCCAGACTTC AGGCAGTGAT GTGTATCATC 
AGTACTATCA TGGAATCCTG CCCCTCCACC TCCAGCTTCT ACAGTAGTGC TACAGCCAAG 
ACTCAGCATA ATGGCATGAA CAACATCATT CGACTGTTCC TAAAGAAGGG ACTAGTCAAT 
GACCTGGCCA GAGTTCCTCA CAGCCTAGAC CTGTCCAGTC CCAATATGGC CAACACAGTC 
AATGCTGCTC TAAAACCCTT GGAAACACTT TCCCGGATTG TAAATCAACC CAGTAGCCTT 
TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC AAGTCTGAGC AGGATGCTCA AGGAGCTTCT 
CAGGACTCCA GCAGCCATCA GCAAGATCCA GGCGAGCCTG GGGAGGCAGA AGTGCAGGAG 
GAGGATCATG ATGTCACTCA GACAGAGGTG GCAGATGGGG ATATCATGGA TGGGGAGGCT 
GAAACCGACT CAGTGGTGAT TGCCGGGCAG CCTGAGGTGC TCAGTTCACA AGAGATGCAG 
GTTGAAAATG AACTGGAGGA CCTGATAGAT GAGTTGCTTG AGAGGGATGG CGGATCTGGG 
AACAGTACAA TTATAGTGAG CAGAAGTGGA GAGGATGAAT CACAAGAGGA CGTGCTGATG 
GATGAAGCTC CTTCCAACCT CAGCCAAGCT TCCACCTTGC AGGCCAACCG AGAAGATTCC 
ATGAATATCC TGGACCCTGA GGATGAGGAG GAACATACTC AGGAAGAGGA CAGCAGTGGC 
AGTAACGAGG ATGAGGATGA TAGTCAGGAT GAAGAGGAGG AGGAGGAGGA AGATGAGGAA 
GATGATCAGG AGGATGATGA AGGTGAAGAA GGAGATGAAG ATGATGACGA CGATGGTTCT 
GAGATGGAAT TGGATGAGGA TTACCCTGAT ATGAACGCTT CTCCCTTGGT CCGATTTGAG 
CGCTTTGACC GGGAGGATGA TCTCATCATT GAGTTTGACA ACATGTTCTC CAGTGCTACA 
GACATTCCCC CATCCCCAGG AAATATCCCT ACCACCCACC CATTGATGGT ACGTCATGCA 
GATCACAGTT CCCTGACACT GGGCAGTGGT TCTTCCACAA CTCGTCTTAC CCAGGGCATC 
GGTCGCAGTC AAAGGACCCT CAGACAGCTC ACTGCCAATA CAGGCCATAC CATTCATGTT 
CACTACCCTG GGAATCGCCA GCCTAACCCT CCTCTTATAC TCCAGAGGTT GCTTGGTCCC 
TCTGCAGCTG CTGACATTCT TCAGCTGAGC AGCAGCCTTC CATTACAAAG CCGAGGCCGG 
GCCCGCCTCC TAGTGGGCAA TGATGACGTC CATATCATCG CCAGGTCTGA TGACGAGCTG 
CTGGATGACT TTTTTCATGA CCAGAGTACA GCCACTAGCC AAGCAGGAAC CCTGTCCAGC 
ATCCCAACAG CTCTAACCCG ATGGACAGAG GAATGCAAAG TTCTTGATGC TGAGAGTATG 
CATGACTGTG TTTCAGTGGT TAAAGTGCCC ATTGTCAATC ACCTGGAATT CTTGAGAGAT 
GAGGAGCTAG AAGAGAGAAG GGAGAAACGC AGGAAACAAT TGGCTGAGGA AGAAACAAAA 
ATAATAGACA AAGGCAAAGA AGACAAGGAG AACAGAGATC AGAGTGCACA GTGTACTGTA 
ACTAAGACAA ATGACTCCAC TGAACAGAAT GTCTCAGATG GGACTCCTAT GCCTGACAGC 
TATCCAACAA CCCCCTCTTC AACGGATGCA CCTACATCTG AGTCCAAGGA AACACTTGGT 
ACCCTACAAC CCTCCCAGCA ACAGCCAGCA CTTCCACCCC CACCATCCTT AGGAGAGATT 
CCTCAGGAAC TACAGTCCCC TGCTGAAGAA GTGGCAAACT CTACACAGCT ATTGATGCCT 
ATAGAGTTGG AGGAATTGGG TCCCACAAGA CCAAGTGGAG AAGCAGAAAC AACTCAAATG 
GAATTGTCTC CAGCTCCCAC CATAACCTCT CTTTCCCCAG AGAGAGCTGA AGATTCAGAC 
GCGTTAACAG CTGTCAGCAG TCAGCTGGAA GGTTCTCCCA TGGACACCAG CAGCCTGGCT 
TCCTGTACGT TAGAAGAGGC TGTGGGTGAC ACTCCAGCAG CTGGCAGTTC TGAGCAGCCC 
ACAGCAGGTA GTTCCACTCC TGGGGATGCG CCATCAGTTG TGGCAGAAGT GCAAGGCAGA 
CCTGATGTAT CAAGAGAATC CAACCAGCCA CCTGAGGACA GCTCCCCCCC TGCATCCTCG 
GAGAGTTCTT CCACTAGAGA TTCTGCCGTG GCCATCTCTG GAGCAGATTC TCGTGGAATT 
CTAGAAGAGC CACTACCTTC AACAAGCAGT GAAGAAGAAG ATCCCCTTGC TGGTATCAGT 
CTCCCTGAAG GTGTGGATCC CTCCTTTCTG GCTGCTCTCC CTGATGATAT CCGTCGGGAA 
GTCCTACAGA ACCAGCTGGG CATACGTCCA CCAACCCGAA GTGCTCCCTC CTCAAATAGC 
TCAGCTCCTG CAGTAGTGGG GAACCCTGGT GTAACTGAAG TGAGCCCTGA GTTCCTGGCT 
GCCCTACCTC CAGCTATTCA AGAGGAAGTG CTAGCACAGC AGAGAGCTGA ACAGCAGCGA 
CGGGAACTGG CTCAGAATGC GAGCTCAGAC ACCCCCATGG ACCCTGTGAC TTTCATTCAG 
ACTTTGCCCT CAGATCTGCG CCGTAGTGTC CTAGAGGATA TGGAAGACAG TGTGTTAGCT 
GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCTCTAA GACGAGAACA AGAAGCCCGA 
CAGCGGCAGC TCATGCATGA GCGTCTTTTT GGACACAGTA GTACTTCTGC ACTCTCTGCC 
ATTCTCCGAA GCCCGGCTTT TACCAGTCGC CTGAGTGGCA ACCGTGGGGT CCAGTATACT 
CGCCTTGCTG TACAGAGGGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAATAGG 
CCTTCTGGCA GTAATGTGGA CACACTACTC CGCCTCCGAG GACGACTCCT TCTAGACCAT 
GAAGCCCTGT CTTGCCTCTT GGTCCTACTT TTTGTGGATG AGCCAAAGCT CAATACTAGC 
CGTCTACACC GAGTACTGAG AAATCTCTGC TATCATGCCC AGACCCGCCA CTGGGTCATC 
CGCAGCCTCC TGTCCATTTT GCAGCGCAGC AGTGAAAGTG AGTTATGCAT TGAGACACCT 
AAGCTCTCCA CAAGTGAAGA AAGGGGCAAG AAATCAAGCA AGAGCTGTGC ATCAAGCAGT 
CATGAGAACC GTCCCCTGGA TCTGCTCCAC AAGATGGAGT CCAAAAGTTC CAATCAGCTC 
TCCTGGCTCT CAGTATCAAT GGATGCTGCT TTAGGCTGTA GGACTAACAT ATTCCAGATT 
CAACGTTCTG GAGGGCGTAA ACATACAGAG AAGCATGCAA GCAGTGGTTC CACTGTCCAC 
ATCCACCCCC AGGCTGCTCC TGTTGTTTGC AGACATGTTT TGGATACACT CATTCAGTTG 
GCCAAGGTGT TTCCCAGCCA CTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT 
GATCGGGAAC GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CCTCACAGTC CTCCAGCAGT 
GGCATCTGTA CAGACTTCTG GGACTTATTG GTAAAACTGG ACAACATGAA TGTTAGCCGA 
AAAGGCAAGA ACTCCGTGAA GTCAGTACCA GTGAGCTCTG GTGGTGAAGG GGAAACTTCC 
CCACACAGCC TTGAAGCCTC TCCACTGGGG CAGCTCATGA ACATGTTGTC ACATCCAGTC 
ATCCGCCGGA GTTCTCTCTT AACTGAGAAA CTCCTGAGAC TCCTCTCTCT CATCTCAATT 
GCTCTCCCAG AAAACAAAGT ATCAGAAGTG CAAACTAACT CCAGCAACAG TGGTTCCTCC 
ACTGCTGCCA CCTCAAACAC ATCTACTACT ACCACCACCA CCACTACTGC CACTGCCCCC 
ACGCCCACAC CCCCAGCTGC AACTACCCCT GTCACTTCTG CTCCAGCTCT GGTTGCTGCC 
ACAGCTATTT CCACCATTAC TGTAGCTGCT TCTACCACAG TGACTACCCC CACAACTGCT 
ACCACTACTG TTTCAACTTC TACTACTAAA GGCAGCAAAT CTCCAGCAAA GGTTGGTGAA 
GGAGGCAGTG GTATAGACTT CAAAATGGTG TCCTCTGGCC TCACTGAAAA CCAGCTACAG 
CTCTCTGTAG AGGTGTTGAC ATCCCACTCT TGTTCTGAAG AAGGCTTGGA AGATGCGGCC 
AATGTGTTAC TGCAGCTCTC TCGTGGGGAC TCTGGTACCC GGGACACTGT TCTTAAGCTG 
CTACTAAATG GAGCCCGCCA TCTGGGTTAT ACCCTCTGTA AACAGATAGG TACCCTACTC 
GCTGAACTAC GGGAATATAA TCTGGAGCAG CAGCGGCGAG CCCAATGCGA GACCCTCTCT 
CCTGATGGCC TACCTGAGGA GCAACCACAG ACCACCAAAC TGAAAGGCAA AATGCAGAGC 
AGGTTTGACA TGGCTGAAAA TGTGGTAATT GTGGCATCTC AGAAGCGACC CTTGGGTGGC 
CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACATCTAC CCAGAAGTTC 
TTCTTGAGGG TACTACAGGT CATCATCCAG CTGCGGGATG ACACACGACG AGCTAACAAG 
AAAGCCAAGC AGACAGGCAG GCTAGGTTCC TCCGGTTTAG GCTCAGCTAG CAGCATCCAG 
GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT 
CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CTTCTAACCA GTCAGAGACA 
TCTGTCCGGA GAGAGGAATC GCCCATGGAT GTGGACCAGC CATCTCCTAG TGCTCAAGAT 
ACTCAGTCCA TTGTAATCTC AGATGGAACA CCACAGGGAG AGAAGGAGAA AGAGGAGAAA 
CCACCTGAGT TACCCCTGCT CAGTGAGCAA CTGAGCCTAG ATGAACTGTG GGACATGCTA 
GGGGAATGTT TAAAAGAACT GGAGGAATCT CATGACCAGC ATGCAGTATT AGTGCTACAG 
CCTGCTGTCG AGGCCTTCTT TTTGGTCCAT GCCACAGAGC GGGAGAGCAA GCCTCCTGTC 
CGAGATACCC GTGAGAGCCA GCTGGCACAC ATCAAGGATG AACCTCCTCC ACTCTCCCCC 
GCCCCCTTAA CCCCAGCCAC TCCATCCTCC CTTGACCCAT TCTTCTCCAG GGAGCCCTCA 
TCTATGCACA TTTCCTCGAG CCTGCCCCCT GACACACAGA AGTTCCTTCG CTTTGCAGAG 
ACTCACCGCA CTGTGTTAAA CCAGATACTA CGGCAGTCTA CCACCCATCT TGCTGATGGG 
CCTTTTGCTG TCTTGGTAGA TTATATCCGT GTCCTCGACT TTGATGTCAA GCGCAAATAT 
TTCCGCCAAG AGCTGGAGCG TTTGGACGAA GGGCTCCGGA AAGAAGATAT GGCAGTGCAT 
GTCCGCCGTG ACCATGTATT TGAAGACTCC TATCGTGAGC TGCATCGCAA ATCTCCTGAA 
GAAATGAAGA ATCGATTGTA TATAGTGTTT GAAGGAGAAG AAGGGCAGGA TGCTGGGGGG 
CTCCTGCGAG AGTGGTACAT GATCATCTCT CGAGAGATGT TCAACCCTAT GTATGCCTTG 
TTCCGTACCT CACCTGGTGA CCGGGTCACC TACACCATCA ATCCATCTTC CCACTGTAAC 
CCCAACCACC TCAGCTACTT CAAGTTTGTT GGACGAATTG TAGCCAAAGC TGTATATGAC 
AACCGCCTCC TGGAGTGCTA CTTTACTAGG TCTTTCTACA AACACATCTT GGGCAAATCT 
GTCAGGTATA CAGATATGGA GAGTGAAGAT TACCACTTCT ACCAGGGCCT AGTTTATCTG 
CTTGAAAATG ATGTCTCTAC ATTAGGCTAT GACCTCACCT TCAGCACTGA GGTTCAAGAA 
TTTGGAGTAT GTGAAGTTCG TGACCTCAAA CCCAATGGAG CCAACATCTT GGTAACAGAG 
GAGAACAAGA AGGAATATGT ACACCTGGTT TGCCAGATGA GAATGACAGG AGCCATCCGC 
AAACAGCTGG CAGCCTTCTT GGAGGGCTTC TATGAAATCA TTCCAAAGCG CCTCATATCC 
ATCTTTACTG AGCAGGAATT AGAGCTGCTC ATATCAGGGC TGCCTACCAT CGACATTGAT 
GATCTAAAAT CTAACACTGA GTACCACAAG TACCAATCCA ACTCTATTCA GATCCAGTGG 
TTTTGGAGAG CATTGCGTTC CTTTGACCAA GCAGACCGTG CCAAGTTCCT CCAGTTTGTC 
ACAGGTACTT CCAAGGTACC CCTACAAGGA TTTGCTGCCC TTGAAGGTAT GAATGGCATT 
CAGAAGTTTC AGATTCATCG AGATGACAGA TCCACAGATC GCCTGCCTTC AGCTCACACA 
TGTTTTAATC AACTGGACCT GCCTGCCTAT GAGAGCTTTG AGAAGCTCCG CCACATGCTA 
CTATTGGCCA TCCAGGAATG CTCTGAAGGC TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251444.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107304.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X20, mRNA.

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CloneID	OMu69811
Clone ID Related Accession (Same CDS sequence)	XM_011247855.1 , XM_030251444.1
Accession Version	XM_030251444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG ATGATGAATT TGAGAATCTT AGAATTAAAG GTCCTAATGC TGTACAGCTG 
GTAAAGACAA CACCTTTGAA GCCCTCATCT CTGCCTGTCA TTCCTGATAC TATTAAGGAA 
GTTATCTATG ATATGCTGAA TGCTTTGGCT GCATACCATG CTCCAGAGGA AGCAGACAAA 
TCTGATCCTA AACCTGGGGG AACGACCCAA GAGGTTGGCC AGCTCCTGCA AGACATGGGT 
GACGATGTAT ACCAGCAGTA CCGGTCACTT ACTCGTCAGA GCAGTGACTT TGATACACAG 
TCAGGTTTTT CCTTAAATAG TCAGGTTTTT GCTGCAGATG GTGCCCCTGC TGAAACTTCC 
ACAACTGGAA CCTCCCAAGG AGAGGGAGCT TCAACTCCAG AGGAGACTCG AGAAGGGAAG 
AAAGATAAAG AAGGGGACCG TACATCTGAG GAAGGCAAGC AGAAAAGCAA GGGCAGCAAA 
CCTTTAATGC CTACCTCTAC TATCCTTCGT CTTCTGGCAG AGTTGGTGAG GTCCTATGTT 
GGTATTGCTA CCCTGATTGC AAACTACAGC TATACCGTGG GCCAGTCTGA GCTGATCAAA 
GAGGACTGCA GTGTGCTAGC TTTTGTCCTG GACCACCTCC TCCCACACAC CCAGAATGCA 
GAAGACAAGG ATACACCTGC CCTGGCACGT CTATTCCTTG CAAGTCTGGC TGCTGCGGGG 
AGTGGCACAG ATGCTCAAGT GGCCCTAGTG AATGAAGTGA AAGCAGCCCT GGGACGGGCG 
CTTGCTATGG CTGAGAGTAC AGAGAAGCAC GCCAGACTTC AGGCAGTGAT GTGTATCATC 
AGTACTATCA TGGAATCCTG CCCCTCCACC TCCAGCTTCT ACAGTAGTGC TACAGCCAAG 
ACTCAGCATA ATGGCATGAA CAACATCATT CGACTGTTCC TAAAGAAGGG ACTAGTCAAT 
GACCTGGCCA GAGTTCCTCA CAGCCTAGAC CTGTCCAGTC CCAATATGGC CAACACAGTC 
AATGCTGCTC TAAAACCCTT GGAAACACTT TCCCGGATTG TAAATCAACC CAGTAGCCTT 
TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC AAGTCTGAGC AGGATGCTCA AGGAGCTTCT 
CAGGACTCCA GCAGCCATCA GCAAGATCCA GGCGAGCCTG GGGAGGCAGA AGTGCAGGAG 
GAGGATCATG ATGTCACTCA GACAGAGGTG GCAGATGGGG ATATCATGGA TGGGGAGGCT 
GAAACCGACT CAGTGGTGAT TGCCGGGCAG CCTGAGGTGC TCAGTTCACA AGAGATGCAG 
GTTGAAAATG AACTGGAGGA CCTGATAGAT GAGTTGCTTG AGAGGGATGG CGGATCTGGG 
AACAGTACAA TTATAGTGAG CAGAAGTGGA GAGGATGAAT CACAAGAGGA CGTGCTGATG 
GATGAAGCTC CTTCCAACCT CAGCCAAGCT TCCACCTTGC AGGCCAACCG AGAAGATTCC 
ATGAATATCC TGGACCCTGA GGATGAGGAG GAACATACTC AGGAAGAGGA CAGCAGTGGC 
AGTAACGAGG ATGAGGATGA TAGTCAGGAT GAAGAGGAGG AGGAGGAGGA AGATGAGGAA 
GATGATCAGG AGGATGATGA AGGTGAAGAA GGAGATGAAG ATGATGACGA CGATGGTTCT 
GAGATGGAAT TGGATGAGGA TTACCCTGAT ATGAACGCTT CTCCCTTGGT CCGATTTGAG 
CGCTTTGACC GGGAGGATGA TCTCATCATT GAGTTTGACA ACATGTTCTC CAGTGCTACA 
GACATTCCCC CATCCCCAGG AAATATCCCT ACCACCCACC CATTGATGGT ACGTCATGCA 
GATCACAGTT CCCTGACACT GGGCAGTGGT TCTTCCACAA CTCGTCTTAC CCAGGGCATC 
GGTCGCAGTC AAAGGACCCT CAGACAGCTC ACTGCCAATA CAGGCCATAC CATTCATGTT 
CACTACCCTG GGAATCGCCA GCCTAACCCT CCTCTTATAC TCCAGAGGTT GCTTGGTCCC 
TCTGCAGCTG CTGACATTCT TCAGCTGAGC AGCAGCCTTC CATTACAAAG CCGAGGCCGG 
GCCCGCCTCC TAGTGGGCAA TGATGACGTC CATATCATCG CCAGGTCTGA TGACGAGCTG 
CTGGATGACT TTTTTCATGA CCAGAGTACA GCCACTAGCC AAGCAGGAAC CCTGTCCAGC 
ATCCCAACAG CTCTAACCCG ATGGACAGAG GAATGCAAAG TTCTTGATGC TGAGAGTATG 
CATGACTGTG TTTCAGTGGT TAAAGTGCCC ATTGTCAATC ACCTGGAATT CTTGAGAGAT 
GAGGAGCTAG AAGAGAGAAG GGAGAAACGC AGGAAACAAT TGGCTGAGGA AGAAACAAAA 
ATAATAGACA AAGGCAAAGA AGACAAGGAG AACAGAGATC AGAGTGCACA GTGTACTGTA 
ACTAAGACAA ATGACTCCAC TGAACAGAAT GTCTCAGATG GGACTCCTAT GCCTGACAGC 
TATCCAACAA CCCCCTCTTC AACGGATGCA CCTACATCTG AGTCCAAGGA AACACTTGGT 
ACCCTACAAC CCTCCCAGCA ACAGCCAGCA CTTCCACCCC CACCATCCTT AGGAGAGATT 
CCTCAGGAAC TACAGTCCCC TGCTGAAGAA GTGGCAAACT CTACACAGCT ATTGATGCCT 
ATAGAGTTGG AGGAATTGGG TCCCACAAGA CCAAGTGGAG AAGCAGAAAC AACTCAAATG 
GAATTGTCTC CAGCTCCCAC CATAACCTCT CTTTCCCCAG AGAGAGCTGA AGATTCAGAC 
GCGTTAACAG CTGTCAGCAG TCAGCTGGAA GGTTCTCCCA TGGACACCAG CAGCCTGGCT 
TCCTGTACGT TAGAAGAGGC TGTGGGTGAC ACTCCAGCAG CTGGCAGTTC TGAGCAGCCC 
ACAGCAGGTA GTTCCACTCC TGGGGATGCG CCATCAGTTG TGGCAGAAGT GCAAGGCAGA 
CCTGATGTAT CAAGAGAATC CAACCAGCCA CCTGAGGACA GCTCCCCCCC TGCATCCTCG 
GAGAGTTCTT CCACTAGAGA TTCTGCCGTG GCCATCTCTG GAGCAGATTC TCGTGGAATT 
CTAGAAGAGC CACTACCTTC AACAAGCAGT GAAGAAGAAG ATCCCCTTGC TGGTATCAGT 
CTCCCTGAAG GTGTGGATCC CTCCTTTCTG GCTGCTCTCC CTGATGATAT CCGTCGGGAA 
GTCCTACAGA ACCAGCTGGG CATACGTCCA CCAACCCGAA GTGCTCCCTC CTCAAATAGC 
TCAGCTCCTG CAGTAGTGGG GAACCCTGGT GTAACTGAAG TGAGCCCTGA GTTCCTGGCT 
GCCCTACCTC CAGCTATTCA AGAGGAAGTG CTAGCACAGC AGAGAGCTGA ACAGCAGCGA 
CGGGAACTGG CTCAGAATGC GAGCTCAGAC ACCCCCATGG ACCCTGTGAC TTTCATTCAG 
ACTTTGCCCT CAGATCTGCG CCGTAGTGTC CTAGAGGATA TGGAAGACAG TGTGTTAGCT 
GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCTCTAA GACGAGAACA AGAAGCCCGA 
CAGCGGCAGC TCATGCATGA GCGTCTTTTT GGACACAGTA GTACTTCTGC ACTCTCTGCC 
ATTCTCCGAA GCCCGGCTTT TACCAGTCGC CTGAGTGGCA ACCGTGGGGT CCAGTATACT 
CGCCTTGCTG TACAGAGGGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAATAGG 
CCTTCTGGCA GTAATGTGGA CACACTACTC CGCCTCCGAG GACGACTCCT TCTAGACCAT 
GAAGCCCTGT CTTGCCTCTT GGTCCTACTT TTTGTGGATG AGCCAAAGCT CAATACTAGC 
CGTCTACACC GAGTACTGAG AAATCTCTGC TATCATGCCC AGACCCGCCA CTGGGTCATC 
CGCAGCCTCC TGTCCATTTT GCAGCGCAGC AGTGAAAGTG AGTTATGCAT TGAGACACCT 
AAGCTCTCCA CAAGTGAAGA AAGGGGCAAG AAATCAAGCA AGAGCTGTGC ATCAAGCAGT 
CATGAGAACC GTCCCCTGGA TCTGCTCCAC AAGATGGAGT CCAAAAGTTC CAATCAGCTC 
TCCTGGCTCT CAGTATCAAT GGATGCTGCT TTAGGCTGTA GGACTAACAT ATTCCAGATT 
CAACGTTCTG GAGGGCGTAA ACATACAGAG AAGCATGCAA GCAGTGGTTC CACTGTCCAC 
ATCCACCCCC AGGCTGCTCC TGTTGTTTGC AGACATGTTT TGGATACACT CATTCAGTTG 
GCCAAGGTGT TTCCCAGCCA CTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT 
GATCGGGAAC GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CCTCACAGTC CTCCAGCAGT 
GGCATCTGTA CAGACTTCTG GGACTTATTG GTAAAACTGG ACAACATGAA TGTTAGCCGA 
AAAGGCAAGA ACTCCGTGAA GTCAGTACCA GTGAGCTCTG GTGGTGAAGG GGAAACTTCC 
CCACACAGCC TTGAAGCCTC TCCACTGGGG CAGCTCATGA ACATGTTGTC ACATCCAGTC 
ATCCGCCGGA GTTCTCTCTT AACTGAGAAA CTCCTGAGAC TCCTCTCTCT CATCTCAATT 
GCTCTCCCAG AAAACAAAGT ATCAGAAGTG CAAACTAACT CCAGCAACAG TGGTTCCTCC 
ACTGCTGCCA CCTCAAACAC ATCTACTACT ACCACCACCA CCACTACTGC CACTGCCCCC 
ACGCCCACAC CCCCAGCTGC AACTACCCCT GTCACTTCTG CTCCAGCTCT GGTTGCTGCC 
ACAGCTATTT CCACCATTAC TGTAGCTGCT TCTACCACAG TGACTACCCC CACAACTGCT 
ACCACTACTG TTTCAACTTC TACTACTAAA GGCAGCAAAT CTCCAGCAAA GGTTGGTGAA 
GGAGGCAGTG GTATAGACTT CAAAATGGTG TCCTCTGGCC TCACTGAAAA CCAGCTACAG 
CTCTCTGTAG AGGTGTTGAC ATCCCACTCT TGTTCTGAAG AAGGCTTGGA AGATGCGGCC 
AATGTGTTAC TGCAGCTCTC TCGTGGGGAC TCTGGTACCC GGGACACTGT TCTTAAGCTG 
CTACTAAATG GAGCCCGCCA TCTGGGTTAT ACCCTCTGTA AACAGATAGG TACCCTACTC 
GCTGAACTAC GGGAATATAA TCTGGAGCAG CAGCGGCGAG CCCAATGCGA GACCCTCTCT 
CCTGATGGCC TACCTGAGGA GCAACCACAG ACCACCAAAC TGAAAGGCAA AATGCAGAGC 
AGGTTTGACA TGGCTGAAAA TGTGGTAATT GTGGCATCTC AGAAGCGACC CTTGGGTGGC 
CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACATCTAC CCAGAAGTTC 
TTCTTGAGGG TACTACAGGT CATCATCCAG CTGCGGGATG ACACACGACG AGCTAACAAG 
AAAGCCAAGC AGACAGGCAG GCTAGGTTCC TCCGGTTTAG GCTCAGCTAG CAGCATCCAG 
GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT 
CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CTTCTAACCA GTCAGAGACA 
TCTGTCCGGA GAGAGGAATC GCCCATGGAT GTGGACCAGC CATCTCCTAG TGCTCAAGAT 
ACTCAGTCCA TTGTAATCTC AGATGGAACA CCACAGGGAG AGAAGGAGAA AGAGGAGAAA 
CCACCTGAGT TACCCCTGCT CAGTGAGCAA CTGAGCCTAG ATGAACTGTG GGACATGCTA 
GGGGAATGTT TAAAAGAACT GGAGGAATCT CATGACCAGC ATGCAGTATT AGTGCTACAG 
CCTGCTGTCG AGGCCTTCTT TTTGGTCCAT GCCACAGAGC GGGAGAGCAA GCCTCCTGTC 
CGAGATACCC GTGAGAGCCA GCTGGCACAC ATCAAGGATG AACCTCCTCC ACTCTCCCCC 
GCCCCCTTAA CCCCAGCCAC TCCATCCTCC CTTGACCCAT TCTTCTCCAG GGAGCCCTCA 
TCTATGCACA TTTCCTCGAG CCTGCCCCCT GACACACAGA AGTTCCTTCG CTTTGCAGAG 
ACTCACCGCA CTGTGTTAAA CCAGATACTA CGGCAGTCTA CCACCCATCT TGCTGATGGG 
CCTTTTGCTG TCTTGGTAGA TTATATCCGT GTCCTCGACT TTGATGTCAA GCGCAAATAT 
TTCCGCCAAG AGCTGGAGCG TTTGGACGAA GGGCTCCGGA AAGAAGATAT GGCAGTGCAT 
GTCCGCCGTG ACCATGTATT TGAAGACTCC TATCGTGAGC TGCATCGCAA ATCTCCTGAA 
GAAATGAAGA ATCGATTGTA TATAGTGTTT GAAGGAGAAG AAGGGCAGGA TGCTGGGGGG 
CTCCTGCGAG AGTGGTACAT GATCATCTCT CGAGAGATGT TCAACCCTAT GTATGCCTTG 
TTCCGTACCT CACCTGGTGA CCGGGTCACC TACACCATCA ATCCATCTTC CCACTGTAAC 
CCCAACCACC TCAGCTACTT CAAGTTTGTT GGACGAATTG TAGCCAAAGC TGTATATGAC 
AACCGCCTCC TGGAGTGCTA CTTTACTAGG TCTTTCTACA AACACATCTT GGGCAAATCT 
GTCAGGTATA CAGATATGGA GAGTGAAGAT TACCACTTCT ACCAGGGCCT AGTTTATCTG 
CTTGAAAATG ATGTCTCTAC ATTAGGCTAT GACCTCACCT TCAGCACTGA GGTTCAAGAA 
TTTGGAGTAT GTGAAGTTCG TGACCTCAAA CCCAATGGAG CCAACATCTT GGTAACAGAG 
GAGAACAAGA AGGAATATGT ACACCTGGTT TGCCAGATGA GAATGACAGG AGCCATCCGC 
AAACAGCTGG CAGCCTTCTT GGAGGGCTTC TATGAAATCA TTCCAAAGCG CCTCATATCC 
ATCTTTACTG AGCAGGAATT AGAGCTGCTC ATATCAGGGC TGCCTACCAT CGACATTGAT 
GATCTAAAAT CTAACACTGA GTACCACAAG TACCAATCCA ACTCTATTCA GATCCAGTGG 
TTTTGGAGAG CATTGCGTTC CTTTGACCAA GCAGACCGTG CCAAGTTCCT CCAGTTTGTC 
ACAGGTACTT CCAAGGTACC CCTACAAGGA TTTGCTGCCC TTGAAGGTAT GAATGGCATT 
CAGAAGTTTC AGATTCATCG AGATGACAGA TCCACAGATC GCCTGCCTTC AGCTCACACA 
TGTTTTAATC AACTGGACCT GCCTGCCTAT GAGAGCTTTG AGAAGCTCCG CCACATGCTA 
CTATTGGCCA TCCAGGAATG CTCTGAAGGC TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107304.1
CDS	1856..15220
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251444.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251444.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG ATGATGAATT TGAGAATCTT AGAATTAAAG GTCCTAATGC TGTACAGCTG 
GTAAAGACAA CACCTTTGAA GCCCTCATCT CTGCCTGTCA TTCCTGATAC TATTAAGGAA 
GTTATCTATG ATATGCTGAA TGCTTTGGCT GCATACCATG CTCCAGAGGA AGCAGACAAA 
TCTGATCCTA AACCTGGGGG AACGACCCAA GAGGTTGGCC AGCTCCTGCA AGACATGGGT 
GACGATGTAT ACCAGCAGTA CCGGTCACTT ACTCGTCAGA GCAGTGACTT TGATACACAG 
TCAGGTTTTT CCTTAAATAG TCAGGTTTTT GCTGCAGATG GTGCCCCTGC TGAAACTTCC 
ACAACTGGAA CCTCCCAAGG AGAGGGAGCT TCAACTCCAG AGGAGACTCG AGAAGGGAAG 
AAAGATAAAG AAGGGGACCG TACATCTGAG GAAGGCAAGC AGAAAAGCAA GGGCAGCAAA 
CCTTTAATGC CTACCTCTAC TATCCTTCGT CTTCTGGCAG AGTTGGTGAG GTCCTATGTT 
GGTATTGCTA CCCTGATTGC AAACTACAGC TATACCGTGG GCCAGTCTGA GCTGATCAAA 
GAGGACTGCA GTGTGCTAGC TTTTGTCCTG GACCACCTCC TCCCACACAC CCAGAATGCA 
GAAGACAAGG ATACACCTGC CCTGGCACGT CTATTCCTTG CAAGTCTGGC TGCTGCGGGG 
AGTGGCACAG ATGCTCAAGT GGCCCTAGTG AATGAAGTGA AAGCAGCCCT GGGACGGGCG 
CTTGCTATGG CTGAGAGTAC AGAGAAGCAC GCCAGACTTC AGGCAGTGAT GTGTATCATC 
AGTACTATCA TGGAATCCTG CCCCTCCACC TCCAGCTTCT ACAGTAGTGC TACAGCCAAG 
ACTCAGCATA ATGGCATGAA CAACATCATT CGACTGTTCC TAAAGAAGGG ACTAGTCAAT 
GACCTGGCCA GAGTTCCTCA CAGCCTAGAC CTGTCCAGTC CCAATATGGC CAACACAGTC 
AATGCTGCTC TAAAACCCTT GGAAACACTT TCCCGGATTG TAAATCAACC CAGTAGCCTT 
TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC AAGTCTGAGC AGGATGCTCA AGGAGCTTCT 
CAGGACTCCA GCAGCCATCA GCAAGATCCA GGCGAGCCTG GGGAGGCAGA AGTGCAGGAG 
GAGGATCATG ATGTCACTCA GACAGAGGTG GCAGATGGGG ATATCATGGA TGGGGAGGCT 
GAAACCGACT CAGTGGTGAT TGCCGGGCAG CCTGAGGTGC TCAGTTCACA AGAGATGCAG 
GTTGAAAATG AACTGGAGGA CCTGATAGAT GAGTTGCTTG AGAGGGATGG CGGATCTGGG 
AACAGTACAA TTATAGTGAG CAGAAGTGGA GAGGATGAAT CACAAGAGGA CGTGCTGATG 
GATGAAGCTC CTTCCAACCT CAGCCAAGCT TCCACCTTGC AGGCCAACCG AGAAGATTCC 
ATGAATATCC TGGACCCTGA GGATGAGGAG GAACATACTC AGGAAGAGGA CAGCAGTGGC 
AGTAACGAGG ATGAGGATGA TAGTCAGGAT GAAGAGGAGG AGGAGGAGGA AGATGAGGAA 
GATGATCAGG AGGATGATGA AGGTGAAGAA GGAGATGAAG ATGATGACGA CGATGGTTCT 
GAGATGGAAT TGGATGAGGA TTACCCTGAT ATGAACGCTT CTCCCTTGGT CCGATTTGAG 
CGCTTTGACC GGGAGGATGA TCTCATCATT GAGTTTGACA ACATGTTCTC CAGTGCTACA 
GACATTCCCC CATCCCCAGG AAATATCCCT ACCACCCACC CATTGATGGT ACGTCATGCA 
GATCACAGTT CCCTGACACT GGGCAGTGGT TCTTCCACAA CTCGTCTTAC CCAGGGCATC 
GGTCGCAGTC AAAGGACCCT CAGACAGCTC ACTGCCAATA CAGGCCATAC CATTCATGTT 
CACTACCCTG GGAATCGCCA GCCTAACCCT CCTCTTATAC TCCAGAGGTT GCTTGGTCCC 
TCTGCAGCTG CTGACATTCT TCAGCTGAGC AGCAGCCTTC CATTACAAAG CCGAGGCCGG 
GCCCGCCTCC TAGTGGGCAA TGATGACGTC CATATCATCG CCAGGTCTGA TGACGAGCTG 
CTGGATGACT TTTTTCATGA CCAGAGTACA GCCACTAGCC AAGCAGGAAC CCTGTCCAGC 
ATCCCAACAG CTCTAACCCG ATGGACAGAG GAATGCAAAG TTCTTGATGC TGAGAGTATG 
CATGACTGTG TTTCAGTGGT TAAAGTGCCC ATTGTCAATC ACCTGGAATT CTTGAGAGAT 
GAGGAGCTAG AAGAGAGAAG GGAGAAACGC AGGAAACAAT TGGCTGAGGA AGAAACAAAA 
ATAATAGACA AAGGCAAAGA AGACAAGGAG AACAGAGATC AGAGTGCACA GTGTACTGTA 
ACTAAGACAA ATGACTCCAC TGAACAGAAT GTCTCAGATG GGACTCCTAT GCCTGACAGC 
TATCCAACAA CCCCCTCTTC AACGGATGCA CCTACATCTG AGTCCAAGGA AACACTTGGT 
ACCCTACAAC CCTCCCAGCA ACAGCCAGCA CTTCCACCCC CACCATCCTT AGGAGAGATT 
CCTCAGGAAC TACAGTCCCC TGCTGAAGAA GTGGCAAACT CTACACAGCT ATTGATGCCT 
ATAGAGTTGG AGGAATTGGG TCCCACAAGA CCAAGTGGAG AAGCAGAAAC AACTCAAATG 
GAATTGTCTC CAGCTCCCAC CATAACCTCT CTTTCCCCAG AGAGAGCTGA AGATTCAGAC 
GCGTTAACAG CTGTCAGCAG TCAGCTGGAA GGTTCTCCCA TGGACACCAG CAGCCTGGCT 
TCCTGTACGT TAGAAGAGGC TGTGGGTGAC ACTCCAGCAG CTGGCAGTTC TGAGCAGCCC 
ACAGCAGGTA GTTCCACTCC TGGGGATGCG CCATCAGTTG TGGCAGAAGT GCAAGGCAGA 
CCTGATGTAT CAAGAGAATC CAACCAGCCA CCTGAGGACA GCTCCCCCCC TGCATCCTCG 
GAGAGTTCTT CCACTAGAGA TTCTGCCGTG GCCATCTCTG GAGCAGATTC TCGTGGAATT 
CTAGAAGAGC CACTACCTTC AACAAGCAGT GAAGAAGAAG ATCCCCTTGC TGGTATCAGT 
CTCCCTGAAG GTGTGGATCC CTCCTTTCTG GCTGCTCTCC CTGATGATAT CCGTCGGGAA 
GTCCTACAGA ACCAGCTGGG CATACGTCCA CCAACCCGAA GTGCTCCCTC CTCAAATAGC 
TCAGCTCCTG CAGTAGTGGG GAACCCTGGT GTAACTGAAG TGAGCCCTGA GTTCCTGGCT 
GCCCTACCTC CAGCTATTCA AGAGGAAGTG CTAGCACAGC AGAGAGCTGA ACAGCAGCGA 
CGGGAACTGG CTCAGAATGC GAGCTCAGAC ACCCCCATGG ACCCTGTGAC TTTCATTCAG 
ACTTTGCCCT CAGATCTGCG CCGTAGTGTC CTAGAGGATA TGGAAGACAG TGTGTTAGCT 
GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCTCTAA GACGAGAACA AGAAGCCCGA 
CAGCGGCAGC TCATGCATGA GCGTCTTTTT GGACACAGTA GTACTTCTGC ACTCTCTGCC 
ATTCTCCGAA GCCCGGCTTT TACCAGTCGC CTGAGTGGCA ACCGTGGGGT CCAGTATACT 
CGCCTTGCTG TACAGAGGGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAATAGG 
CCTTCTGGCA GTAATGTGGA CACACTACTC CGCCTCCGAG GACGACTCCT TCTAGACCAT 
GAAGCCCTGT CTTGCCTCTT GGTCCTACTT TTTGTGGATG AGCCAAAGCT CAATACTAGC 
CGTCTACACC GAGTACTGAG AAATCTCTGC TATCATGCCC AGACCCGCCA CTGGGTCATC 
CGCAGCCTCC TGTCCATTTT GCAGCGCAGC AGTGAAAGTG AGTTATGCAT TGAGACACCT 
AAGCTCTCCA CAAGTGAAGA AAGGGGCAAG AAATCAAGCA AGAGCTGTGC ATCAAGCAGT 
CATGAGAACC GTCCCCTGGA TCTGCTCCAC AAGATGGAGT CCAAAAGTTC CAATCAGCTC 
TCCTGGCTCT CAGTATCAAT GGATGCTGCT TTAGGCTGTA GGACTAACAT ATTCCAGATT 
CAACGTTCTG GAGGGCGTAA ACATACAGAG AAGCATGCAA GCAGTGGTTC CACTGTCCAC 
ATCCACCCCC AGGCTGCTCC TGTTGTTTGC AGACATGTTT TGGATACACT CATTCAGTTG 
GCCAAGGTGT TTCCCAGCCA CTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT 
GATCGGGAAC GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CCTCACAGTC CTCCAGCAGT 
GGCATCTGTA CAGACTTCTG GGACTTATTG GTAAAACTGG ACAACATGAA TGTTAGCCGA 
AAAGGCAAGA ACTCCGTGAA GTCAGTACCA GTGAGCTCTG GTGGTGAAGG GGAAACTTCC 
CCACACAGCC TTGAAGCCTC TCCACTGGGG CAGCTCATGA ACATGTTGTC ACATCCAGTC 
ATCCGCCGGA GTTCTCTCTT AACTGAGAAA CTCCTGAGAC TCCTCTCTCT CATCTCAATT 
GCTCTCCCAG AAAACAAAGT ATCAGAAGTG CAAACTAACT CCAGCAACAG TGGTTCCTCC 
ACTGCTGCCA CCTCAAACAC ATCTACTACT ACCACCACCA CCACTACTGC CACTGCCCCC 
ACGCCCACAC CCCCAGCTGC AACTACCCCT GTCACTTCTG CTCCAGCTCT GGTTGCTGCC 
ACAGCTATTT CCACCATTAC TGTAGCTGCT TCTACCACAG TGACTACCCC CACAACTGCT 
ACCACTACTG TTTCAACTTC TACTACTAAA GGCAGCAAAT CTCCAGCAAA GGTTGGTGAA 
GGAGGCAGTG GTATAGACTT CAAAATGGTG TCCTCTGGCC TCACTGAAAA CCAGCTACAG 
CTCTCTGTAG AGGTGTTGAC ATCCCACTCT TGTTCTGAAG AAGGCTTGGA AGATGCGGCC 
AATGTGTTAC TGCAGCTCTC TCGTGGGGAC TCTGGTACCC GGGACACTGT TCTTAAGCTG 
CTACTAAATG GAGCCCGCCA TCTGGGTTAT ACCCTCTGTA AACAGATAGG TACCCTACTC 
GCTGAACTAC GGGAATATAA TCTGGAGCAG CAGCGGCGAG CCCAATGCGA GACCCTCTCT 
CCTGATGGCC TACCTGAGGA GCAACCACAG ACCACCAAAC TGAAAGGCAA AATGCAGAGC 
AGGTTTGACA TGGCTGAAAA TGTGGTAATT GTGGCATCTC AGAAGCGACC CTTGGGTGGC 
CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACATCTAC CCAGAAGTTC 
TTCTTGAGGG TACTACAGGT CATCATCCAG CTGCGGGATG ACACACGACG AGCTAACAAG 
AAAGCCAAGC AGACAGGCAG GCTAGGTTCC TCCGGTTTAG GCTCAGCTAG CAGCATCCAG 
GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT 
CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CTTCTAACCA GTCAGAGACA 
TCTGTCCGGA GAGAGGAATC GCCCATGGAT GTGGACCAGC CATCTCCTAG TGCTCAAGAT 
ACTCAGTCCA TTGTAATCTC AGATGGAACA CCACAGGGAG AGAAGGAGAA AGAGGAGAAA 
CCACCTGAGT TACCCCTGCT CAGTGAGCAA CTGAGCCTAG ATGAACTGTG GGACATGCTA 
GGGGAATGTT TAAAAGAACT GGAGGAATCT CATGACCAGC ATGCAGTATT AGTGCTACAG 
CCTGCTGTCG AGGCCTTCTT TTTGGTCCAT GCCACAGAGC GGGAGAGCAA GCCTCCTGTC 
CGAGATACCC GTGAGAGCCA GCTGGCACAC ATCAAGGATG AACCTCCTCC ACTCTCCCCC 
GCCCCCTTAA CCCCAGCCAC TCCATCCTCC CTTGACCCAT TCTTCTCCAG GGAGCCCTCA 
TCTATGCACA TTTCCTCGAG CCTGCCCCCT GACACACAGA AGTTCCTTCG CTTTGCAGAG 
ACTCACCGCA CTGTGTTAAA CCAGATACTA CGGCAGTCTA CCACCCATCT TGCTGATGGG 
CCTTTTGCTG TCTTGGTAGA TTATATCCGT GTCCTCGACT TTGATGTCAA GCGCAAATAT 
TTCCGCCAAG AGCTGGAGCG TTTGGACGAA GGGCTCCGGA AAGAAGATAT GGCAGTGCAT 
GTCCGCCGTG ACCATGTATT TGAAGACTCC TATCGTGAGC TGCATCGCAA ATCTCCTGAA 
GAAATGAAGA ATCGATTGTA TATAGTGTTT GAAGGAGAAG AAGGGCAGGA TGCTGGGGGG 
CTCCTGCGAG AGTGGTACAT GATCATCTCT CGAGAGATGT TCAACCCTAT GTATGCCTTG 
TTCCGTACCT CACCTGGTGA CCGGGTCACC TACACCATCA ATCCATCTTC CCACTGTAAC 
CCCAACCACC TCAGCTACTT CAAGTTTGTT GGACGAATTG TAGCCAAAGC TGTATATGAC 
AACCGCCTCC TGGAGTGCTA CTTTACTAGG TCTTTCTACA AACACATCTT GGGCAAATCT 
GTCAGGTATA CAGATATGGA GAGTGAAGAT TACCACTTCT ACCAGGGCCT AGTTTATCTG 
CTTGAAAATG ATGTCTCTAC ATTAGGCTAT GACCTCACCT TCAGCACTGA GGTTCAAGAA 
TTTGGAGTAT GTGAAGTTCG TGACCTCAAA CCCAATGGAG CCAACATCTT GGTAACAGAG 
GAGAACAAGA AGGAATATGT ACACCTGGTT TGCCAGATGA GAATGACAGG AGCCATCCGC 
AAACAGCTGG CAGCCTTCTT GGAGGGCTTC TATGAAATCA TTCCAAAGCG CCTCATATCC 
ATCTTTACTG AGCAGGAATT AGAGCTGCTC ATATCAGGGC TGCCTACCAT CGACATTGAT 
GATCTAAAAT CTAACACTGA GTACCACAAG TACCAATCCA ACTCTATTCA GATCCAGTGG 
TTTTGGAGAG CATTGCGTTC CTTTGACCAA GCAGACCGTG CCAAGTTCCT CCAGTTTGTC 
ACAGGTACTT CCAAGGTACC CCTACAAGGA TTTGCTGCCC TTGAAGGTAT GAATGGCATT 
CAGAAGTTTC AGATTCATCG AGATGACAGA TCCACAGATC GCCTGCCTTC AGCTCACACA 
TGTTTTAATC AACTGGACCT GCCTGCCTAT GAGAGCTTTG AGAAGCTCCG CCACATGCTA 
CTATTGGCCA TCCAGGAATG CTCTGAAGGC TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251446.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107306.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X22, mRNA.

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CloneID	OMu69812
Clone ID Related Accession (Same CDS sequence)	XM_030251446.1 , XM_011247856.1
Accession Version	XM_030251446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGAT 
GGAGAACAGG ATGGAACTGC TGGAAGCATG GATGCATCTG CCCAGGGTTT ATTAGAAGGA 
ATTGAACTAG ATGGTGACAC ACTGGCACCC ATGGAGACAG ATGAACCTTC TTCTTCAGAT 
TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCAA GAATTAAACA AATCAAGCCG 
TTGCTGTCAG CTTCTTCCAG ATTAGGCCGA GCACTTGCTG AGCTCTTTGG ACTCCTTGTT 
AAGCTTTGTG TGGGTTCTCC TGTCCGCCAA AGGAGGAGCC ATCATGCTGC CAGTACCACC 
ACTGCACCAA CACCTGCTGC TAGATCAACA GCTTCAGCCC TCACTAAGCT ACTCACAAAA 
GGCTTATCAT GGCAGCCCCC ACCATATACA CCCACTCCCC GCTTCAGGCT GACGTTCTTC 
ATCTGTTCAG TTGGATTCAC CTCTCCAATG CTCTTTGATG AGAGAAAGTA TCCTTACCAC 
CTTATGCTGC AGAAATTTCT CTGCTCTGGT GGTCACAATG CTCTTTTTGA AACTTTCAAC 
TGGGCTCTAT CCATGGGAGG AAAAGTTCCT GTTTCTGAGG GATTGGAACA TTCAGATCTA 
CCTGATGGTA CAGGAGAATT CCTAGATGCC TGGCTTATGC TTGTAGAAAA GATGGTAAAT 
CCCACCACGG TGCTTGAATC TCCACATTCG TTGCCTGCTA AATTGCCTGG AGGAGTCCAG 
AGCTTTCCAC AATTTAGTGC CCTTCGCTTC CTTGTAGTAA CTCAAAAAGC AGCCTTTACT 
TGTATCAAGA ACCTGTGGAA CCGGAAACCC CTTAAAGTAT ATGGGGGAAG AATGGCTGAG 
TCTATGCTGG CCATTTTGTG CCACATTCTC CGAGGGGAAC CTGTGATTCG AGAAAGACTG 
AGCAAAGAGA AGGAAGGATC TCGAGGAGAA GAAGAAGCAG GCCAGGAAGA AGGTGGCTCA 
CGCAGAGAAC CTCAAGTCAA CCAGCAACAG CTACAACAGC TCATGGACAT GGGTTTCACA 
AGAGAACATG CCATGGAGGC ACTGTTGAAT ACCAGTACGA TGGAACAAGC AACAGAGTAC 
CTTTTAACCC ACCCTCCTCC AATCATAGGA GGAGTTGTTC GGGATCTTAG CATGTCAGAA 
GAGGACCAGA TGATGAGAGC AATTGCTATG TCTCTGGGAC AGGATATTCC AATGGATCAA 
AGGGCAGAGT CACCTGAGGA AGTTGCTTGC CGGAAGGAGG AAGAAGAGCG TAAAGCTCGA 
GAAAAACAAG AAGAGGAAGA AGCTAAATGT CTAGAGAAGT TTCAAGATGC TGACCCCTTG 
GAACAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTAC CAGGCTGCTT CCATCTTCTT 
GATGAGTTGC CTGATACAGT GTACCGTGTT TGTGATCTGA TCATGACAGC TATCAAACGT 
AATGGAGCTG ACTATCGTGA TATGATTCTA AAGCAAGTTG TCAACCAGGT GTGGGAAGCT 
GCTGATGTAT TGATCAAAGC TGCTCTTCCT CTAACAACAA GTGACACAAA AACAGTTTCA 
GAGTGGATCA GTCAGATGGC TACCCTGCCA CAGGCCTCCA ATTTGGCTAC TAGAATCTTG 
CTTTTAACAC TACTTTTTGA GGAGTTGAAG TTACCTTGTG CCTGGGTGGT TGAATCAAGT 
GGCATTCTTA ATGTCCTAAT CAAACTCTTA GAAGTGGTTC AACCCTGCCT ACAGGCTGCC 
AAAGAGCAAA AGGAAGTCCA GACCCCAAAG TGGATCACCC CAGTGTTGCT CCTTATTGAT 
TTTTATGAAA AGACAGCCAT CTCATCAAAG AGAAGAGCCC AAATGACTAA GTACCTACAG 
TCCAACAGCA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GGCGTTGGTG TAGTTACAGT 
GCTAGCAACA ACAGCACCAT TGATTCTGCC TGGAAATCTG GAGAAACAAG TGTAAGATTC 
ACTGCTGGCC GACGAAGATA CACTGTCCAG TTCACTACAA TGGTGCAGGT TAATGAAGAA 
ACAGGAAACC GACGCCCTGT GATGCTGACT CTCCTCAGGG TGCCACGACT GAGTAAGAAC 
TCAAAAAGCA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA GGAGACCGAT 
ATCAAACATA AAGAAAATAA AGGCAATGAT ATCCCCTTAG CTCTAGAGAG CACAAACACT 
GAAAAAGAGG CAAGCCTGGA TGAAACAAAA ATTGGGGAGA TCCTAATCCA GGGCCTGACA 
GAAGATATGG TGACTGTTCT AATCCGTGCC TGTGTGAGCA TGCTGGGAGT CCCTGTGGAC 
CCAGACACTT TGCATGCCAC CCTTCGTCTC TGTTTAAGGC TCACCCGAGA CCACAAATAT 
GCCATGATGT TTGCAGAACT GAAGAGTACA CGAATGATCT TGAATTTGAC CCAGAGCTCA 
GGTTTCAATG GGTTTACCCC CTTAGTCACC CTTCTCTTAA GGCACATCAT TGAGGATCCT 
TGTACTCTTC GTCATACTAT GGAAAAGGTT GTTCGCTCGG CAGCCACAAG TGGAGCTGGT 
AGTACTACAT CTGGTGTTGT ATCTGGCAGC CTCGGTTCTC GGGAGATCAA CTATATCCTT 
AGAGTCCTAG GACCAGCTGC CTGCCGCAAT CCAGACATAT TCACAGAAGT AGCCAATTGC 
TGTATCCGCA TTGCCCTTCC AGCCCCTCGA GGCTCAGGAA CTGCTTCAGA TGATGAATTT 
GAGAATCTTA GAATTAAAGG TCCTAATGCT GTACAGCTGG TAAAGACAAC ACCTTTGAAG 
CCCTCATCTC TGCCTGTCAT TCCTGATACT ATTAAGGAAG TTATCTATGA TATGCTGAAT 
GCTTTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCTGGGGGA 
ACGACCCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ACGATGTATA CCAGCAGTAC 
CGGTCACTTA CTCGTCAGAG CAGTGACTTT GATACACAGT CAGGTTTTTC CTTAAATAGT 
CAGGTTTTTG CTGCAGATGG TGCCCCTGCT GAAACTTCCA CAACTGGAAC CTCCCAAGGA 
GAGGGAGCTT CAACTCCAGA GGAGACTCGA GAAGGGAAGA AAGATAAAGA AGGGGACCGT 
ACATCTGAGG AAGGCAAGCA GAAAAGCAAG GGCAGCAAAC CTTTAATGCC TACCTCTACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCA 
AACTACAGCT ATACCGTGGG CCAGTCTGAG CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTCCTGG ACCACCTCCT CCCACACACC CAGAATGCAG AAGACAAGGA TACACCTGCC 
CTGGCACGTC TATTCCTTGC AAGTCTGGCT GCTGCGGGGA GTGGCACAGA TGCTCAAGTG 
GCCCTAGTGA ATGAAGTGAA AGCAGCCCTG GGACGGGCGC TTGCTATGGC TGAGAGTACA 
GAGAAGCACG CCAGACTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAATCCTGC 
CCCTCCACCT CCAGCTTCTA CAGTAGTGCT ACAGCCAAGA CTCAGCATAA TGGCATGAAC 
AACATCATTC GACTGTTCCT AAAGAAGGGA CTAGTCAATG ACCTGGCCAG AGTTCCTCAC 
AGCCTAGACC TGTCCAGTCC CAATATGGCC AACACAGTCA ATGCTGCTCT AAAACCCTTG 
GAAACACTTT CCCGGATTGT AAATCAACCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCTCAA GGAGCTTCTC AGGACTCCAG CAGCCATCAG 
CAAGATCCAG GCGAGCCTGG GGAGGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCCGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAAAATGA ACTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AACATACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAAG GAGATGAAGA TGATGACGAC GATGGTTCTG AGATGGAATT GGATGAGGAT 
TACCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATTCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCACCC ATTGATGGTA CGTCATGCAG ATCACAGTTC CCTGACACTG 
GGCAGTGGTT CTTCCACAAC TCGTCTTACC CAGGGCATCG GTCGCAGTCA AAGGACCCTC 
AGACAGCTCA CTGCCAATAC AGGCCATACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCTAACCCTC CTCTTATACT CCAGAGGTTG CTTGGTCCCT CTGCAGCTGC TGACATTCTT 
CAGCTGAGCA GCAGCCTTCC ATTACAAAGC CGAGGCCGGG CCCGCCTCCT AGTGGGCAAT 
GATGACGTCC ATATCATCGC CAGGTCTGAT GACGAGCTGC TGGATGACTT TTTTCATGAC 
CAGAGTACAG CCACTAGCCA AGCAGGAACC CTGTCCAGCA TCCCAACAGC TCTAACCCGA 
TGGACAGAGG AATGCAAAGT TCTTGATGCT GAGAGTATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGCCCA TTGTCAATCA CCTGGAATTC TTGAGAGATG AGGAGCTAGA AGAGAGAAGG 
GAGAAACGCA GGAAACAATT GGCTGAGGAA GAAACAAAAA TAATAGACAA AGGCAAAGAA 
GACAAGGAGA ACAGAGATCA GAGTGCACAG TGTACTGTAA CTAAGACAAA TGACTCCACT 
GAACAGAATG TCTCAGATGG GACTCCTATG CCTGACAGCT ATCCAACAAC CCCCTCTTCA 
ACGGATGCAC CTACATCTGA GTCCAAGGAA ACACTTGGTA CCCTACAACC CTCCCAGCAA 
CAGCCAGCAC TTCCACCCCC ACCATCCTTA GGAGAGATTC CTCAGGAACT ACAGTCCCCT 
GCTGAAGAAG TGGCAAACTC TACACAGCTA TTGATGCCTA TAGAGTTGGA GGAATTGGGT 
CCCACAAGAC CAAGTGGAGA AGCAGAAACA ACTCAAATGG AATTGTCTCC AGCTCCCACC 
ATAACCTCTC TTTCCCCAGA GAGAGCTGAA GATTCAGACG CGTTAACAGC TGTCAGCAGT 
CAGCTGGAAG GTTCTCCCAT GGACACCAGC AGCCTGGCTT CCTGTACGTT AGAAGAGGCT 
GTGGGTGACA CTCCAGCAGC TGGCAGTTCT GAGCAGCCCA CAGCAGGTAG TTCCACTCCT 
GGGGATGCGC CATCAGTTGT GGCAGAAGTG CAAGGCAGAC CTGATGTATC AAGAGAATCC 
AACCAGCCAC CTGAGGACAG CTCCCCCCCT GCATCCTCGG AGAGTTCTTC CACTAGAGAT 
TCTGCCGTGG CCATCTCTGG AGCAGATTCT CGTGGAATTC TAGAAGAGCC ACTACCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCT GGTATCAGTC TCCCTGAAGG TGTGGATCCC 
TCCTTTCTGG CTGCTCTCCC TGATGATATC CGTCGGGAAG TCCTACAGAA CCAGCTGGGC 
ATACGTCCAC CAACCCGAAG TGCTCCCTCC TCAAATAGCT CAGCTCCTGC AGTAGTGGGG 
AACCCTGGTG TAACTGAAGT GAGCCCTGAG TTCCTGGCTG CCCTACCTCC AGCTATTCAA 
GAGGAAGTGC TAGCACAGCA GAGAGCTGAA CAGCAGCGAC GGGAACTGGC TCAGAATGCG 
AGCTCAGACA CCCCCATGGA CCCTGTGACT TTCATTCAGA CTTTGCCCTC AGATCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAAGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCTCTAAG ACGAGAACAA GAAGCCCGAC AGCGGCAGCT CATGCATGAG 
CGTCTTTTTG GACACAGTAG TACTTCTGCA CTCTCTGCCA TTCTCCGAAG CCCGGCTTTT 
ACCAGTCGCC TGAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT ACAGAGGGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAATAGGC CTTCTGGCAG TAATGTGGAC 
ACACTACTCC GCCTCCGAGG ACGACTCCTT CTAGACCATG AAGCCCTGTC TTGCCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ATCATGCCCA GACCCGCCAC TGGGTCATCC GCAGCCTCCT GTCCATTTTG 
CAGCGCAGCA GTGAAAGTGA GTTATGCATT GAGACACCTA AGCTCTCCAC AAGTGAAGAA 
AGGGGCAAGA AATCAAGCAA GAGCTGTGCA TCAAGCAGTC ATGAGAACCG TCCCCTGGAT 
CTGCTCCACA AGATGGAGTC CAAAAGTTCC AATCAGCTCT CCTGGCTCTC AGTATCAATG 
GATGCTGCTT TAGGCTGTAG GACTAACATA TTCCAGATTC AACGTTCTGG AGGGCGTAAA 
CATACAGAGA AGCATGCAAG CAGTGGTTCC ACTGTCCACA TCCACCCCCA GGCTGCTCCT 
GTTGTTTGCA GACATGTTTT GGATACACTC ATTCAGTTGG CCAAGGTGTT TCCCAGCCAC 
TTTACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAACG GGGCAGTAAG 
CAGGCCTGCA GCCCATGCTC CTCACAGTCC TCCAGCAGTG GCATCTGTAC AGACTTCTGG 
GACTTATTGG TAAAACTGGA CAACATGAAT GTTAGCCGAA AAGGCAAGAA CTCCGTGAAG 
TCAGTACCAG TGAGCTCTGG TGGTGAAGGG GAAACTTCCC CACACAGCCT TGAAGCCTCT 
CCACTGGGGC AGCTCATGAA CATGTTGTCA CATCCAGTCA TCCGCCGGAG TTCTCTCTTA 
ACTGAGAAAC TCCTGAGACT CCTCTCTCTC ATCTCAATTG CTCTCCCAGA AAACAAAGTA 
TCAGAAGTGC AAACTAACTC CAGCAACAGT GGTTCCTCCA CTGCTGCCAC CTCAAACACA 
TCTACTACTA CCACCACCAC CACTACTGCC ACTGCCCCCA CGCCCACACC CCCAGCTGCA 
ACTACCCCTG TCACTTCTGC TCCAGCTCTG GTTGCTGCCA CAGCTATTTC CACCATTACT 
GTAGCTGCTT CTACCACAGT GACTACCCCC ACAACTGCTA CCACTACTGT TTCAACTTCT 
ACTACTAAAG GCAGCAAATC TCCAGCAAAG GTTGGTGAAG GAGGCAGTGG TATAGACTTC 
AAAATGGTGT CCTCTGGCCT CACTGAAAAC CAGCTACAGC TCTCTGTAGA GGTGTTGACA 
TCCCACTCTT GTTCTGAAGA AGGCTTGGAA GATGCGGCCA ATGTGTTACT GCAGCTCTCT 
CGTGGGGACT CTGGTACCCG GGACACTGTT CTTAAGCTGC TACTAAATGG AGCCCGCCAT 
CTGGGTTATA CCCTCTGTAA ACAGATAGGT ACCCTACTCG CTGAACTACG GGAATATAAT 
CTGGAGCAGC AGCGGCGAGC CCAATGCGAG ACCCTCTCTC CTGATGGCCT ACCTGAGGAG 
CAACCACAGA CCACCAAACT GAAAGGCAAA ATGCAGAGCA GGTTTGACAT GGCTGAAAAT 
GTGGTAATTG TGGCATCTCA GAAGCGACCC TTGGGTGGCC GGGAGCTCCA GCTGCCTTCT 
ATGTCCATGT TGACATCCAA GACATCTACC CAGAAGTTCT TCTTGAGGGT ACTACAGGTC 
ATCATCCAGC TGCGGGATGA CACACGACGA GCTAACAAGA AAGCCAAGCA GACAGGCAGG 
CTAGGTTCCT CCGGTTTAGG CTCAGCTAGC AGCATCCAGG CAGCTGTTCG GCAGCTGGAG 
GCTGAGGCTG ATGCCATTAT ACAAATGGTA CGTGAGGGTC AAAGGGCGCG GAGACAGCAA 
CAAGCAGCAA CGTCGGAGTC TTCTAACCAG TCAGAGACAT CTGTCCGGAG AGAGGAATCG 
CCCATGGATG TGGACCAGCC ATCTCCTAGT GCTCAAGATA CTCAGTCCAT TGTAATCTCA 
GATGGAACAC CACAGGGAGA GAAGGAGAAA GAGGAGAAAC CACCTGAGTT ACCCCTGCTC 
AGTGAGCAAC TGAGCCTAGA TGAACTGTGG GACATGCTAG GGGAATGTTT AAAAGAACTG 
GAGGAATCTC ATGACCAGCA TGCAGTATTA GTGCTACAGC CTGCTGTCGA GGCCTTCTTT 
TTGGTCCATG CCACAGAGCG GGAGAGCAAG CCTCCTGTCC GAGATACCCG TGAGAGCCAG 
CTGGCACACA TCAAGGATGA ACCTCCTCCA CTCTCCCCCG CCCCCTTAAC CCCAGCCACT 
CCATCCTCCC TTGACCCATT CTTCTCCAGG GAGCCCTCAT CTATGCACAT TTCCTCGAGC 
CTGCCCCCTG ACACACAGAA GTTCCTTCGC TTTGCAGAGA CTCACCGCAC TGTGTTAAAC 
CAGATACTAC GGCAGTCTAC CACCCATCTT GCTGATGGGC CTTTTGCTGT CTTGGTAGAT 
TATATCCGTG TCCTCGACTT TGATGTCAAG CGCAAATATT TCCGCCAAGA GCTGGAGCGT 
TTGGACGAAG GGCTCCGGAA AGAAGATATG GCAGTGCATG TCCGCCGTGA CCATGTATTT 
GAAGACTCCT ATCGTGAGCT GCATCGCAAA TCTCCTGAAG AAATGAAGAA TCGATTGTAT 
ATAGTGTTTG AAGGAGAAGA AGGGCAGGAT GCTGGGGGGC TCCTGCGAGA GTGGTACATG 
ATCATCTCTC GAGAGATGTT CAACCCTATG TATGCCTTGT TCCGTACCTC ACCTGGTGAC 
CGGGTCACCT ACACCATCAA TCCATCTTCC CACTGTAACC CCAACCACCT CAGCTACTTC 
AAGTTTGTTG GACGAATTGT AGCCAAAGCT GTATATGACA ACCGCCTCCT GGAGTGCTAC 
TTTACTAGGT CTTTCTACAA ACACATCTTG GGCAAATCTG TCAGGTATAC AGATATGGAG 
AGTGAAGATT ACCACTTCTA CCAGGGCCTA GTTTATCTGC TTGAAAATGA TGTCTCTACA 
TTAGGCTATG ACCTCACCTT CAGCACTGAG GTTCAAGAAT TTGGAGTATG TGAAGTTCGT 
GACCTCAAAC CCAATGGAGC CAACATCTTG GTAACAGAGG AGAACAAGAA GGAATATGTA 
CACCTGGTTT GCCAGATGAG AATGACAGGA GCCATCCGCA AACAGCTGGC AGCCTTCTTG 
GAGGGCTTCT ATGAAATCAT TCCAAAGCGC CTCATATCCA TCTTTACTGA GCAGGAATTA 
GAGCTGCTCA TATCAGGGCT GCCTACCATC GACATTGATG ATCTAAAATC TAACACTGAG 
TACCACAAGT ACCAATCCAA CTCTATTCAG ATCCAGTGGT TTTGGAGAGC ATTGCGTTCC 
TTTGACCAAG CAGACCGTGC CAAGTTCCTC CAGTTTGTCA CAGGTACTTC CAAGGTACCC 
CTACAAGGAT TTGCTGCCCT TGAAGGTATG AATGGCATTC AGAAGTTTCA GATTCATCGA 
GATGACAGAT CCACAGATCG CCTGCCTTCA GCTCACACAT GTTTTAATCA ACTGGACCTG 
CCTGCCTATG AGAGCTTTGA GAAGCTCCGC CACATGCTAC TATTGGCCAT CCAGGAATGC 
TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107306.1
CDS	1856..15199
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKADGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251446.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251446.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGAT 
GGAGAACAGG ATGGAACTGC TGGAAGCATG GATGCATCTG CCCAGGGTTT ATTAGAAGGA 
ATTGAACTAG ATGGTGACAC ACTGGCACCC ATGGAGACAG ATGAACCTTC TTCTTCAGAT 
TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCAA GAATTAAACA AATCAAGCCG 
TTGCTGTCAG CTTCTTCCAG ATTAGGCCGA GCACTTGCTG AGCTCTTTGG ACTCCTTGTT 
AAGCTTTGTG TGGGTTCTCC TGTCCGCCAA AGGAGGAGCC ATCATGCTGC CAGTACCACC 
ACTGCACCAA CACCTGCTGC TAGATCAACA GCTTCAGCCC TCACTAAGCT ACTCACAAAA 
GGCTTATCAT GGCAGCCCCC ACCATATACA CCCACTCCCC GCTTCAGGCT GACGTTCTTC 
ATCTGTTCAG TTGGATTCAC CTCTCCAATG CTCTTTGATG AGAGAAAGTA TCCTTACCAC 
CTTATGCTGC AGAAATTTCT CTGCTCTGGT GGTCACAATG CTCTTTTTGA AACTTTCAAC 
TGGGCTCTAT CCATGGGAGG AAAAGTTCCT GTTTCTGAGG GATTGGAACA TTCAGATCTA 
CCTGATGGTA CAGGAGAATT CCTAGATGCC TGGCTTATGC TTGTAGAAAA GATGGTAAAT 
CCCACCACGG TGCTTGAATC TCCACATTCG TTGCCTGCTA AATTGCCTGG AGGAGTCCAG 
AGCTTTCCAC AATTTAGTGC CCTTCGCTTC CTTGTAGTAA CTCAAAAAGC AGCCTTTACT 
TGTATCAAGA ACCTGTGGAA CCGGAAACCC CTTAAAGTAT ATGGGGGAAG AATGGCTGAG 
TCTATGCTGG CCATTTTGTG CCACATTCTC CGAGGGGAAC CTGTGATTCG AGAAAGACTG 
AGCAAAGAGA AGGAAGGATC TCGAGGAGAA GAAGAAGCAG GCCAGGAAGA AGGTGGCTCA 
CGCAGAGAAC CTCAAGTCAA CCAGCAACAG CTACAACAGC TCATGGACAT GGGTTTCACA 
AGAGAACATG CCATGGAGGC ACTGTTGAAT ACCAGTACGA TGGAACAAGC AACAGAGTAC 
CTTTTAACCC ACCCTCCTCC AATCATAGGA GGAGTTGTTC GGGATCTTAG CATGTCAGAA 
GAGGACCAGA TGATGAGAGC AATTGCTATG TCTCTGGGAC AGGATATTCC AATGGATCAA 
AGGGCAGAGT CACCTGAGGA AGTTGCTTGC CGGAAGGAGG AAGAAGAGCG TAAAGCTCGA 
GAAAAACAAG AAGAGGAAGA AGCTAAATGT CTAGAGAAGT TTCAAGATGC TGACCCCTTG 
GAACAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTAC CAGGCTGCTT CCATCTTCTT 
GATGAGTTGC CTGATACAGT GTACCGTGTT TGTGATCTGA TCATGACAGC TATCAAACGT 
AATGGAGCTG ACTATCGTGA TATGATTCTA AAGCAAGTTG TCAACCAGGT GTGGGAAGCT 
GCTGATGTAT TGATCAAAGC TGCTCTTCCT CTAACAACAA GTGACACAAA AACAGTTTCA 
GAGTGGATCA GTCAGATGGC TACCCTGCCA CAGGCCTCCA ATTTGGCTAC TAGAATCTTG 
CTTTTAACAC TACTTTTTGA GGAGTTGAAG TTACCTTGTG CCTGGGTGGT TGAATCAAGT 
GGCATTCTTA ATGTCCTAAT CAAACTCTTA GAAGTGGTTC AACCCTGCCT ACAGGCTGCC 
AAAGAGCAAA AGGAAGTCCA GACCCCAAAG TGGATCACCC CAGTGTTGCT CCTTATTGAT 
TTTTATGAAA AGACAGCCAT CTCATCAAAG AGAAGAGCCC AAATGACTAA GTACCTACAG 
TCCAACAGCA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GGCGTTGGTG TAGTTACAGT 
GCTAGCAACA ACAGCACCAT TGATTCTGCC TGGAAATCTG GAGAAACAAG TGTAAGATTC 
ACTGCTGGCC GACGAAGATA CACTGTCCAG TTCACTACAA TGGTGCAGGT TAATGAAGAA 
ACAGGAAACC GACGCCCTGT GATGCTGACT CTCCTCAGGG TGCCACGACT GAGTAAGAAC 
TCAAAAAGCA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA GGAGACCGAT 
ATCAAACATA AAGAAAATAA AGGCAATGAT ATCCCCTTAG CTCTAGAGAG CACAAACACT 
GAAAAAGAGG CAAGCCTGGA TGAAACAAAA ATTGGGGAGA TCCTAATCCA GGGCCTGACA 
GAAGATATGG TGACTGTTCT AATCCGTGCC TGTGTGAGCA TGCTGGGAGT CCCTGTGGAC 
CCAGACACTT TGCATGCCAC CCTTCGTCTC TGTTTAAGGC TCACCCGAGA CCACAAATAT 
GCCATGATGT TTGCAGAACT GAAGAGTACA CGAATGATCT TGAATTTGAC CCAGAGCTCA 
GGTTTCAATG GGTTTACCCC CTTAGTCACC CTTCTCTTAA GGCACATCAT TGAGGATCCT 
TGTACTCTTC GTCATACTAT GGAAAAGGTT GTTCGCTCGG CAGCCACAAG TGGAGCTGGT 
AGTACTACAT CTGGTGTTGT ATCTGGCAGC CTCGGTTCTC GGGAGATCAA CTATATCCTT 
AGAGTCCTAG GACCAGCTGC CTGCCGCAAT CCAGACATAT TCACAGAAGT AGCCAATTGC 
TGTATCCGCA TTGCCCTTCC AGCCCCTCGA GGCTCAGGAA CTGCTTCAGA TGATGAATTT 
GAGAATCTTA GAATTAAAGG TCCTAATGCT GTACAGCTGG TAAAGACAAC ACCTTTGAAG 
CCCTCATCTC TGCCTGTCAT TCCTGATACT ATTAAGGAAG TTATCTATGA TATGCTGAAT 
GCTTTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCTGGGGGA 
ACGACCCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ACGATGTATA CCAGCAGTAC 
CGGTCACTTA CTCGTCAGAG CAGTGACTTT GATACACAGT CAGGTTTTTC CTTAAATAGT 
CAGGTTTTTG CTGCAGATGG TGCCCCTGCT GAAACTTCCA CAACTGGAAC CTCCCAAGGA 
GAGGGAGCTT CAACTCCAGA GGAGACTCGA GAAGGGAAGA AAGATAAAGA AGGGGACCGT 
ACATCTGAGG AAGGCAAGCA GAAAAGCAAG GGCAGCAAAC CTTTAATGCC TACCTCTACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCA 
AACTACAGCT ATACCGTGGG CCAGTCTGAG CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTCCTGG ACCACCTCCT CCCACACACC CAGAATGCAG AAGACAAGGA TACACCTGCC 
CTGGCACGTC TATTCCTTGC AAGTCTGGCT GCTGCGGGGA GTGGCACAGA TGCTCAAGTG 
GCCCTAGTGA ATGAAGTGAA AGCAGCCCTG GGACGGGCGC TTGCTATGGC TGAGAGTACA 
GAGAAGCACG CCAGACTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAATCCTGC 
CCCTCCACCT CCAGCTTCTA CAGTAGTGCT ACAGCCAAGA CTCAGCATAA TGGCATGAAC 
AACATCATTC GACTGTTCCT AAAGAAGGGA CTAGTCAATG ACCTGGCCAG AGTTCCTCAC 
AGCCTAGACC TGTCCAGTCC CAATATGGCC AACACAGTCA ATGCTGCTCT AAAACCCTTG 
GAAACACTTT CCCGGATTGT AAATCAACCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCTCAA GGAGCTTCTC AGGACTCCAG CAGCCATCAG 
CAAGATCCAG GCGAGCCTGG GGAGGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCCGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAAAATGA ACTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AACATACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAAG GAGATGAAGA TGATGACGAC GATGGTTCTG AGATGGAATT GGATGAGGAT 
TACCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATTCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCACCC ATTGATGGTA CGTCATGCAG ATCACAGTTC CCTGACACTG 
GGCAGTGGTT CTTCCACAAC TCGTCTTACC CAGGGCATCG GTCGCAGTCA AAGGACCCTC 
AGACAGCTCA CTGCCAATAC AGGCCATACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCTAACCCTC CTCTTATACT CCAGAGGTTG CTTGGTCCCT CTGCAGCTGC TGACATTCTT 
CAGCTGAGCA GCAGCCTTCC ATTACAAAGC CGAGGCCGGG CCCGCCTCCT AGTGGGCAAT 
GATGACGTCC ATATCATCGC CAGGTCTGAT GACGAGCTGC TGGATGACTT TTTTCATGAC 
CAGAGTACAG CCACTAGCCA AGCAGGAACC CTGTCCAGCA TCCCAACAGC TCTAACCCGA 
TGGACAGAGG AATGCAAAGT TCTTGATGCT GAGAGTATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGCCCA TTGTCAATCA CCTGGAATTC TTGAGAGATG AGGAGCTAGA AGAGAGAAGG 
GAGAAACGCA GGAAACAATT GGCTGAGGAA GAAACAAAAA TAATAGACAA AGGCAAAGAA 
GACAAGGAGA ACAGAGATCA GAGTGCACAG TGTACTGTAA CTAAGACAAA TGACTCCACT 
GAACAGAATG TCTCAGATGG GACTCCTATG CCTGACAGCT ATCCAACAAC CCCCTCTTCA 
ACGGATGCAC CTACATCTGA GTCCAAGGAA ACACTTGGTA CCCTACAACC CTCCCAGCAA 
CAGCCAGCAC TTCCACCCCC ACCATCCTTA GGAGAGATTC CTCAGGAACT ACAGTCCCCT 
GCTGAAGAAG TGGCAAACTC TACACAGCTA TTGATGCCTA TAGAGTTGGA GGAATTGGGT 
CCCACAAGAC CAAGTGGAGA AGCAGAAACA ACTCAAATGG AATTGTCTCC AGCTCCCACC 
ATAACCTCTC TTTCCCCAGA GAGAGCTGAA GATTCAGACG CGTTAACAGC TGTCAGCAGT 
CAGCTGGAAG GTTCTCCCAT GGACACCAGC AGCCTGGCTT CCTGTACGTT AGAAGAGGCT 
GTGGGTGACA CTCCAGCAGC TGGCAGTTCT GAGCAGCCCA CAGCAGGTAG TTCCACTCCT 
GGGGATGCGC CATCAGTTGT GGCAGAAGTG CAAGGCAGAC CTGATGTATC AAGAGAATCC 
AACCAGCCAC CTGAGGACAG CTCCCCCCCT GCATCCTCGG AGAGTTCTTC CACTAGAGAT 
TCTGCCGTGG CCATCTCTGG AGCAGATTCT CGTGGAATTC TAGAAGAGCC ACTACCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCT GGTATCAGTC TCCCTGAAGG TGTGGATCCC 
TCCTTTCTGG CTGCTCTCCC TGATGATATC CGTCGGGAAG TCCTACAGAA CCAGCTGGGC 
ATACGTCCAC CAACCCGAAG TGCTCCCTCC TCAAATAGCT CAGCTCCTGC AGTAGTGGGG 
AACCCTGGTG TAACTGAAGT GAGCCCTGAG TTCCTGGCTG CCCTACCTCC AGCTATTCAA 
GAGGAAGTGC TAGCACAGCA GAGAGCTGAA CAGCAGCGAC GGGAACTGGC TCAGAATGCG 
AGCTCAGACA CCCCCATGGA CCCTGTGACT TTCATTCAGA CTTTGCCCTC AGATCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAAGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCTCTAAG ACGAGAACAA GAAGCCCGAC AGCGGCAGCT CATGCATGAG 
CGTCTTTTTG GACACAGTAG TACTTCTGCA CTCTCTGCCA TTCTCCGAAG CCCGGCTTTT 
ACCAGTCGCC TGAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT ACAGAGGGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAATAGGC CTTCTGGCAG TAATGTGGAC 
ACACTACTCC GCCTCCGAGG ACGACTCCTT CTAGACCATG AAGCCCTGTC TTGCCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ATCATGCCCA GACCCGCCAC TGGGTCATCC GCAGCCTCCT GTCCATTTTG 
CAGCGCAGCA GTGAAAGTGA GTTATGCATT GAGACACCTA AGCTCTCCAC AAGTGAAGAA 
AGGGGCAAGA AATCAAGCAA GAGCTGTGCA TCAAGCAGTC ATGAGAACCG TCCCCTGGAT 
CTGCTCCACA AGATGGAGTC CAAAAGTTCC AATCAGCTCT CCTGGCTCTC AGTATCAATG 
GATGCTGCTT TAGGCTGTAG GACTAACATA TTCCAGATTC AACGTTCTGG AGGGCGTAAA 
CATACAGAGA AGCATGCAAG CAGTGGTTCC ACTGTCCACA TCCACCCCCA GGCTGCTCCT 
GTTGTTTGCA GACATGTTTT GGATACACTC ATTCAGTTGG CCAAGGTGTT TCCCAGCCAC 
TTTACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAACG GGGCAGTAAG 
CAGGCCTGCA GCCCATGCTC CTCACAGTCC TCCAGCAGTG GCATCTGTAC AGACTTCTGG 
GACTTATTGG TAAAACTGGA CAACATGAAT GTTAGCCGAA AAGGCAAGAA CTCCGTGAAG 
TCAGTACCAG TGAGCTCTGG TGGTGAAGGG GAAACTTCCC CACACAGCCT TGAAGCCTCT 
CCACTGGGGC AGCTCATGAA CATGTTGTCA CATCCAGTCA TCCGCCGGAG TTCTCTCTTA 
ACTGAGAAAC TCCTGAGACT CCTCTCTCTC ATCTCAATTG CTCTCCCAGA AAACAAAGTA 
TCAGAAGTGC AAACTAACTC CAGCAACAGT GGTTCCTCCA CTGCTGCCAC CTCAAACACA 
TCTACTACTA CCACCACCAC CACTACTGCC ACTGCCCCCA CGCCCACACC CCCAGCTGCA 
ACTACCCCTG TCACTTCTGC TCCAGCTCTG GTTGCTGCCA CAGCTATTTC CACCATTACT 
GTAGCTGCTT CTACCACAGT GACTACCCCC ACAACTGCTA CCACTACTGT TTCAACTTCT 
ACTACTAAAG GCAGCAAATC TCCAGCAAAG GTTGGTGAAG GAGGCAGTGG TATAGACTTC 
AAAATGGTGT CCTCTGGCCT CACTGAAAAC CAGCTACAGC TCTCTGTAGA GGTGTTGACA 
TCCCACTCTT GTTCTGAAGA AGGCTTGGAA GATGCGGCCA ATGTGTTACT GCAGCTCTCT 
CGTGGGGACT CTGGTACCCG GGACACTGTT CTTAAGCTGC TACTAAATGG AGCCCGCCAT 
CTGGGTTATA CCCTCTGTAA ACAGATAGGT ACCCTACTCG CTGAACTACG GGAATATAAT 
CTGGAGCAGC AGCGGCGAGC CCAATGCGAG ACCCTCTCTC CTGATGGCCT ACCTGAGGAG 
CAACCACAGA CCACCAAACT GAAAGGCAAA ATGCAGAGCA GGTTTGACAT GGCTGAAAAT 
GTGGTAATTG TGGCATCTCA GAAGCGACCC TTGGGTGGCC GGGAGCTCCA GCTGCCTTCT 
ATGTCCATGT TGACATCCAA GACATCTACC CAGAAGTTCT TCTTGAGGGT ACTACAGGTC 
ATCATCCAGC TGCGGGATGA CACACGACGA GCTAACAAGA AAGCCAAGCA GACAGGCAGG 
CTAGGTTCCT CCGGTTTAGG CTCAGCTAGC AGCATCCAGG CAGCTGTTCG GCAGCTGGAG 
GCTGAGGCTG ATGCCATTAT ACAAATGGTA CGTGAGGGTC AAAGGGCGCG GAGACAGCAA 
CAAGCAGCAA CGTCGGAGTC TTCTAACCAG TCAGAGACAT CTGTCCGGAG AGAGGAATCG 
CCCATGGATG TGGACCAGCC ATCTCCTAGT GCTCAAGATA CTCAGTCCAT TGTAATCTCA 
GATGGAACAC CACAGGGAGA GAAGGAGAAA GAGGAGAAAC CACCTGAGTT ACCCCTGCTC 
AGTGAGCAAC TGAGCCTAGA TGAACTGTGG GACATGCTAG GGGAATGTTT AAAAGAACTG 
GAGGAATCTC ATGACCAGCA TGCAGTATTA GTGCTACAGC CTGCTGTCGA GGCCTTCTTT 
TTGGTCCATG CCACAGAGCG GGAGAGCAAG CCTCCTGTCC GAGATACCCG TGAGAGCCAG 
CTGGCACACA TCAAGGATGA ACCTCCTCCA CTCTCCCCCG CCCCCTTAAC CCCAGCCACT 
CCATCCTCCC TTGACCCATT CTTCTCCAGG GAGCCCTCAT CTATGCACAT TTCCTCGAGC 
CTGCCCCCTG ACACACAGAA GTTCCTTCGC TTTGCAGAGA CTCACCGCAC TGTGTTAAAC 
CAGATACTAC GGCAGTCTAC CACCCATCTT GCTGATGGGC CTTTTGCTGT CTTGGTAGAT 
TATATCCGTG TCCTCGACTT TGATGTCAAG CGCAAATATT TCCGCCAAGA GCTGGAGCGT 
TTGGACGAAG GGCTCCGGAA AGAAGATATG GCAGTGCATG TCCGCCGTGA CCATGTATTT 
GAAGACTCCT ATCGTGAGCT GCATCGCAAA TCTCCTGAAG AAATGAAGAA TCGATTGTAT 
ATAGTGTTTG AAGGAGAAGA AGGGCAGGAT GCTGGGGGGC TCCTGCGAGA GTGGTACATG 
ATCATCTCTC GAGAGATGTT CAACCCTATG TATGCCTTGT TCCGTACCTC ACCTGGTGAC 
CGGGTCACCT ACACCATCAA TCCATCTTCC CACTGTAACC CCAACCACCT CAGCTACTTC 
AAGTTTGTTG GACGAATTGT AGCCAAAGCT GTATATGACA ACCGCCTCCT GGAGTGCTAC 
TTTACTAGGT CTTTCTACAA ACACATCTTG GGCAAATCTG TCAGGTATAC AGATATGGAG 
AGTGAAGATT ACCACTTCTA CCAGGGCCTA GTTTATCTGC TTGAAAATGA TGTCTCTACA 
TTAGGCTATG ACCTCACCTT CAGCACTGAG GTTCAAGAAT TTGGAGTATG TGAAGTTCGT 
GACCTCAAAC CCAATGGAGC CAACATCTTG GTAACAGAGG AGAACAAGAA GGAATATGTA 
CACCTGGTTT GCCAGATGAG AATGACAGGA GCCATCCGCA AACAGCTGGC AGCCTTCTTG 
GAGGGCTTCT ATGAAATCAT TCCAAAGCGC CTCATATCCA TCTTTACTGA GCAGGAATTA 
GAGCTGCTCA TATCAGGGCT GCCTACCATC GACATTGATG ATCTAAAATC TAACACTGAG 
TACCACAAGT ACCAATCCAA CTCTATTCAG ATCCAGTGGT TTTGGAGAGC ATTGCGTTCC 
TTTGACCAAG CAGACCGTGC CAAGTTCCTC CAGTTTGTCA CAGGTACTTC CAAGGTACCC 
CTACAAGGAT TTGCTGCCCT TGAAGGTATG AATGGCATTC AGAAGTTTCA GATTCATCGA 
GATGACAGAT CCACAGATCG CCTGCCTTCA GCTCACACAT GTTTTAATCA ACTGGACCTG 
CCTGCCTATG AGAGCTTTGA GAAGCTCCGC CACATGCTAC TATTGGCCAT CCAGGAATGC 
TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_011247856.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246158.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X15, mRNA.

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CloneID	OMu69812
Clone ID Related Accession (Same CDS sequence)	XM_030251446.1 , XM_011247856.1
Accession Version	XM_011247856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGAT 
GGAGAACAGG ATGGAACTGC TGGAAGCATG GATGCATCTG CCCAGGGTTT ATTAGAAGGA 
ATTGAACTAG ATGGTGACAC ACTGGCACCC ATGGAGACAG ATGAACCTTC TTCTTCAGAT 
TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCAA GAATTAAACA AATCAAGCCG 
TTGCTGTCAG CTTCTTCCAG ATTAGGCCGA GCACTTGCTG AGCTCTTTGG ACTCCTTGTT 
AAGCTTTGTG TGGGTTCTCC TGTCCGCCAA AGGAGGAGCC ATCATGCTGC CAGTACCACC 
ACTGCACCAA CACCTGCTGC TAGATCAACA GCTTCAGCCC TCACTAAGCT ACTCACAAAA 
GGCTTATCAT GGCAGCCCCC ACCATATACA CCCACTCCCC GCTTCAGGCT GACGTTCTTC 
ATCTGTTCAG TTGGATTCAC CTCTCCAATG CTCTTTGATG AGAGAAAGTA TCCTTACCAC 
CTTATGCTGC AGAAATTTCT CTGCTCTGGT GGTCACAATG CTCTTTTTGA AACTTTCAAC 
TGGGCTCTAT CCATGGGAGG AAAAGTTCCT GTTTCTGAGG GATTGGAACA TTCAGATCTA 
CCTGATGGTA CAGGAGAATT CCTAGATGCC TGGCTTATGC TTGTAGAAAA GATGGTAAAT 
CCCACCACGG TGCTTGAATC TCCACATTCG TTGCCTGCTA AATTGCCTGG AGGAGTCCAG 
AGCTTTCCAC AATTTAGTGC CCTTCGCTTC CTTGTAGTAA CTCAAAAAGC AGCCTTTACT 
TGTATCAAGA ACCTGTGGAA CCGGAAACCC CTTAAAGTAT ATGGGGGAAG AATGGCTGAG 
TCTATGCTGG CCATTTTGTG CCACATTCTC CGAGGGGAAC CTGTGATTCG AGAAAGACTG 
AGCAAAGAGA AGGAAGGATC TCGAGGAGAA GAAGAAGCAG GCCAGGAAGA AGGTGGCTCA 
CGCAGAGAAC CTCAAGTCAA CCAGCAACAG CTACAACAGC TCATGGACAT GGGTTTCACA 
AGAGAACATG CCATGGAGGC ACTGTTGAAT ACCAGTACGA TGGAACAAGC AACAGAGTAC 
CTTTTAACCC ACCCTCCTCC AATCATAGGA GGAGTTGTTC GGGATCTTAG CATGTCAGAA 
GAGGACCAGA TGATGAGAGC AATTGCTATG TCTCTGGGAC AGGATATTCC AATGGATCAA 
AGGGCAGAGT CACCTGAGGA AGTTGCTTGC CGGAAGGAGG AAGAAGAGCG TAAAGCTCGA 
GAAAAACAAG AAGAGGAAGA AGCTAAATGT CTAGAGAAGT TTCAAGATGC TGACCCCTTG 
GAACAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTAC CAGGCTGCTT CCATCTTCTT 
GATGAGTTGC CTGATACAGT GTACCGTGTT TGTGATCTGA TCATGACAGC TATCAAACGT 
AATGGAGCTG ACTATCGTGA TATGATTCTA AAGCAAGTTG TCAACCAGGT GTGGGAAGCT 
GCTGATGTAT TGATCAAAGC TGCTCTTCCT CTAACAACAA GTGACACAAA AACAGTTTCA 
GAGTGGATCA GTCAGATGGC TACCCTGCCA CAGGCCTCCA ATTTGGCTAC TAGAATCTTG 
CTTTTAACAC TACTTTTTGA GGAGTTGAAG TTACCTTGTG CCTGGGTGGT TGAATCAAGT 
GGCATTCTTA ATGTCCTAAT CAAACTCTTA GAAGTGGTTC AACCCTGCCT ACAGGCTGCC 
AAAGAGCAAA AGGAAGTCCA GACCCCAAAG TGGATCACCC CAGTGTTGCT CCTTATTGAT 
TTTTATGAAA AGACAGCCAT CTCATCAAAG AGAAGAGCCC AAATGACTAA GTACCTACAG 
TCCAACAGCA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GGCGTTGGTG TAGTTACAGT 
GCTAGCAACA ACAGCACCAT TGATTCTGCC TGGAAATCTG GAGAAACAAG TGTAAGATTC 
ACTGCTGGCC GACGAAGATA CACTGTCCAG TTCACTACAA TGGTGCAGGT TAATGAAGAA 
ACAGGAAACC GACGCCCTGT GATGCTGACT CTCCTCAGGG TGCCACGACT GAGTAAGAAC 
TCAAAAAGCA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA GGAGACCGAT 
ATCAAACATA AAGAAAATAA AGGCAATGAT ATCCCCTTAG CTCTAGAGAG CACAAACACT 
GAAAAAGAGG CAAGCCTGGA TGAAACAAAA ATTGGGGAGA TCCTAATCCA GGGCCTGACA 
GAAGATATGG TGACTGTTCT AATCCGTGCC TGTGTGAGCA TGCTGGGAGT CCCTGTGGAC 
CCAGACACTT TGCATGCCAC CCTTCGTCTC TGTTTAAGGC TCACCCGAGA CCACAAATAT 
GCCATGATGT TTGCAGAACT GAAGAGTACA CGAATGATCT TGAATTTGAC CCAGAGCTCA 
GGTTTCAATG GGTTTACCCC CTTAGTCACC CTTCTCTTAA GGCACATCAT TGAGGATCCT 
TGTACTCTTC GTCATACTAT GGAAAAGGTT GTTCGCTCGG CAGCCACAAG TGGAGCTGGT 
AGTACTACAT CTGGTGTTGT ATCTGGCAGC CTCGGTTCTC GGGAGATCAA CTATATCCTT 
AGAGTCCTAG GACCAGCTGC CTGCCGCAAT CCAGACATAT TCACAGAAGT AGCCAATTGC 
TGTATCCGCA TTGCCCTTCC AGCCCCTCGA GGCTCAGGAA CTGCTTCAGA TGATGAATTT 
GAGAATCTTA GAATTAAAGG TCCTAATGCT GTACAGCTGG TAAAGACAAC ACCTTTGAAG 
CCCTCATCTC TGCCTGTCAT TCCTGATACT ATTAAGGAAG TTATCTATGA TATGCTGAAT 
GCTTTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCTGGGGGA 
ACGACCCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ACGATGTATA CCAGCAGTAC 
CGGTCACTTA CTCGTCAGAG CAGTGACTTT GATACACAGT CAGGTTTTTC CTTAAATAGT 
CAGGTTTTTG CTGCAGATGG TGCCCCTGCT GAAACTTCCA CAACTGGAAC CTCCCAAGGA 
GAGGGAGCTT CAACTCCAGA GGAGACTCGA GAAGGGAAGA AAGATAAAGA AGGGGACCGT 
ACATCTGAGG AAGGCAAGCA GAAAAGCAAG GGCAGCAAAC CTTTAATGCC TACCTCTACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCA 
AACTACAGCT ATACCGTGGG CCAGTCTGAG CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTCCTGG ACCACCTCCT CCCACACACC CAGAATGCAG AAGACAAGGA TACACCTGCC 
CTGGCACGTC TATTCCTTGC AAGTCTGGCT GCTGCGGGGA GTGGCACAGA TGCTCAAGTG 
GCCCTAGTGA ATGAAGTGAA AGCAGCCCTG GGACGGGCGC TTGCTATGGC TGAGAGTACA 
GAGAAGCACG CCAGACTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAATCCTGC 
CCCTCCACCT CCAGCTTCTA CAGTAGTGCT ACAGCCAAGA CTCAGCATAA TGGCATGAAC 
AACATCATTC GACTGTTCCT AAAGAAGGGA CTAGTCAATG ACCTGGCCAG AGTTCCTCAC 
AGCCTAGACC TGTCCAGTCC CAATATGGCC AACACAGTCA ATGCTGCTCT AAAACCCTTG 
GAAACACTTT CCCGGATTGT AAATCAACCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCTCAA GGAGCTTCTC AGGACTCCAG CAGCCATCAG 
CAAGATCCAG GCGAGCCTGG GGAGGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCCGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAAAATGA ACTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AACATACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAAG GAGATGAAGA TGATGACGAC GATGGTTCTG AGATGGAATT GGATGAGGAT 
TACCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATTCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCACCC ATTGATGGTA CGTCATGCAG ATCACAGTTC CCTGACACTG 
GGCAGTGGTT CTTCCACAAC TCGTCTTACC CAGGGCATCG GTCGCAGTCA AAGGACCCTC 
AGACAGCTCA CTGCCAATAC AGGCCATACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCTAACCCTC CTCTTATACT CCAGAGGTTG CTTGGTCCCT CTGCAGCTGC TGACATTCTT 
CAGCTGAGCA GCAGCCTTCC ATTACAAAGC CGAGGCCGGG CCCGCCTCCT AGTGGGCAAT 
GATGACGTCC ATATCATCGC CAGGTCTGAT GACGAGCTGC TGGATGACTT TTTTCATGAC 
CAGAGTACAG CCACTAGCCA AGCAGGAACC CTGTCCAGCA TCCCAACAGC TCTAACCCGA 
TGGACAGAGG AATGCAAAGT TCTTGATGCT GAGAGTATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGCCCA TTGTCAATCA CCTGGAATTC TTGAGAGATG AGGAGCTAGA AGAGAGAAGG 
GAGAAACGCA GGAAACAATT GGCTGAGGAA GAAACAAAAA TAATAGACAA AGGCAAAGAA 
GACAAGGAGA ACAGAGATCA GAGTGCACAG TGTACTGTAA CTAAGACAAA TGACTCCACT 
GAACAGAATG TCTCAGATGG GACTCCTATG CCTGACAGCT ATCCAACAAC CCCCTCTTCA 
ACGGATGCAC CTACATCTGA GTCCAAGGAA ACACTTGGTA CCCTACAACC CTCCCAGCAA 
CAGCCAGCAC TTCCACCCCC ACCATCCTTA GGAGAGATTC CTCAGGAACT ACAGTCCCCT 
GCTGAAGAAG TGGCAAACTC TACACAGCTA TTGATGCCTA TAGAGTTGGA GGAATTGGGT 
CCCACAAGAC CAAGTGGAGA AGCAGAAACA ACTCAAATGG AATTGTCTCC AGCTCCCACC 
ATAACCTCTC TTTCCCCAGA GAGAGCTGAA GATTCAGACG CGTTAACAGC TGTCAGCAGT 
CAGCTGGAAG GTTCTCCCAT GGACACCAGC AGCCTGGCTT CCTGTACGTT AGAAGAGGCT 
GTGGGTGACA CTCCAGCAGC TGGCAGTTCT GAGCAGCCCA CAGCAGGTAG TTCCACTCCT 
GGGGATGCGC CATCAGTTGT GGCAGAAGTG CAAGGCAGAC CTGATGTATC AAGAGAATCC 
AACCAGCCAC CTGAGGACAG CTCCCCCCCT GCATCCTCGG AGAGTTCTTC CACTAGAGAT 
TCTGCCGTGG CCATCTCTGG AGCAGATTCT CGTGGAATTC TAGAAGAGCC ACTACCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCT GGTATCAGTC TCCCTGAAGG TGTGGATCCC 
TCCTTTCTGG CTGCTCTCCC TGATGATATC CGTCGGGAAG TCCTACAGAA CCAGCTGGGC 
ATACGTCCAC CAACCCGAAG TGCTCCCTCC TCAAATAGCT CAGCTCCTGC AGTAGTGGGG 
AACCCTGGTG TAACTGAAGT GAGCCCTGAG TTCCTGGCTG CCCTACCTCC AGCTATTCAA 
GAGGAAGTGC TAGCACAGCA GAGAGCTGAA CAGCAGCGAC GGGAACTGGC TCAGAATGCG 
AGCTCAGACA CCCCCATGGA CCCTGTGACT TTCATTCAGA CTTTGCCCTC AGATCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAAGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCTCTAAG ACGAGAACAA GAAGCCCGAC AGCGGCAGCT CATGCATGAG 
CGTCTTTTTG GACACAGTAG TACTTCTGCA CTCTCTGCCA TTCTCCGAAG CCCGGCTTTT 
ACCAGTCGCC TGAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT ACAGAGGGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAATAGGC CTTCTGGCAG TAATGTGGAC 
ACACTACTCC GCCTCCGAGG ACGACTCCTT CTAGACCATG AAGCCCTGTC TTGCCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ATCATGCCCA GACCCGCCAC TGGGTCATCC GCAGCCTCCT GTCCATTTTG 
CAGCGCAGCA GTGAAAGTGA GTTATGCATT GAGACACCTA AGCTCTCCAC AAGTGAAGAA 
AGGGGCAAGA AATCAAGCAA GAGCTGTGCA TCAAGCAGTC ATGAGAACCG TCCCCTGGAT 
CTGCTCCACA AGATGGAGTC CAAAAGTTCC AATCAGCTCT CCTGGCTCTC AGTATCAATG 
GATGCTGCTT TAGGCTGTAG GACTAACATA TTCCAGATTC AACGTTCTGG AGGGCGTAAA 
CATACAGAGA AGCATGCAAG CAGTGGTTCC ACTGTCCACA TCCACCCCCA GGCTGCTCCT 
GTTGTTTGCA GACATGTTTT GGATACACTC ATTCAGTTGG CCAAGGTGTT TCCCAGCCAC 
TTTACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAACG GGGCAGTAAG 
CAGGCCTGCA GCCCATGCTC CTCACAGTCC TCCAGCAGTG GCATCTGTAC AGACTTCTGG 
GACTTATTGG TAAAACTGGA CAACATGAAT GTTAGCCGAA AAGGCAAGAA CTCCGTGAAG 
TCAGTACCAG TGAGCTCTGG TGGTGAAGGG GAAACTTCCC CACACAGCCT TGAAGCCTCT 
CCACTGGGGC AGCTCATGAA CATGTTGTCA CATCCAGTCA TCCGCCGGAG TTCTCTCTTA 
ACTGAGAAAC TCCTGAGACT CCTCTCTCTC ATCTCAATTG CTCTCCCAGA AAACAAAGTA 
TCAGAAGTGC AAACTAACTC CAGCAACAGT GGTTCCTCCA CTGCTGCCAC CTCAAACACA 
TCTACTACTA CCACCACCAC CACTACTGCC ACTGCCCCCA CGCCCACACC CCCAGCTGCA 
ACTACCCCTG TCACTTCTGC TCCAGCTCTG GTTGCTGCCA CAGCTATTTC CACCATTACT 
GTAGCTGCTT CTACCACAGT GACTACCCCC ACAACTGCTA CCACTACTGT TTCAACTTCT 
ACTACTAAAG GCAGCAAATC TCCAGCAAAG GTTGGTGAAG GAGGCAGTGG TATAGACTTC 
AAAATGGTGT CCTCTGGCCT CACTGAAAAC CAGCTACAGC TCTCTGTAGA GGTGTTGACA 
TCCCACTCTT GTTCTGAAGA AGGCTTGGAA GATGCGGCCA ATGTGTTACT GCAGCTCTCT 
CGTGGGGACT CTGGTACCCG GGACACTGTT CTTAAGCTGC TACTAAATGG AGCCCGCCAT 
CTGGGTTATA CCCTCTGTAA ACAGATAGGT ACCCTACTCG CTGAACTACG GGAATATAAT 
CTGGAGCAGC AGCGGCGAGC CCAATGCGAG ACCCTCTCTC CTGATGGCCT ACCTGAGGAG 
CAACCACAGA CCACCAAACT GAAAGGCAAA ATGCAGAGCA GGTTTGACAT GGCTGAAAAT 
GTGGTAATTG TGGCATCTCA GAAGCGACCC TTGGGTGGCC GGGAGCTCCA GCTGCCTTCT 
ATGTCCATGT TGACATCCAA GACATCTACC CAGAAGTTCT TCTTGAGGGT ACTACAGGTC 
ATCATCCAGC TGCGGGATGA CACACGACGA GCTAACAAGA AAGCCAAGCA GACAGGCAGG 
CTAGGTTCCT CCGGTTTAGG CTCAGCTAGC AGCATCCAGG CAGCTGTTCG GCAGCTGGAG 
GCTGAGGCTG ATGCCATTAT ACAAATGGTA CGTGAGGGTC AAAGGGCGCG GAGACAGCAA 
CAAGCAGCAA CGTCGGAGTC TTCTAACCAG TCAGAGACAT CTGTCCGGAG AGAGGAATCG 
CCCATGGATG TGGACCAGCC ATCTCCTAGT GCTCAAGATA CTCAGTCCAT TGTAATCTCA 
GATGGAACAC CACAGGGAGA GAAGGAGAAA GAGGAGAAAC CACCTGAGTT ACCCCTGCTC 
AGTGAGCAAC TGAGCCTAGA TGAACTGTGG GACATGCTAG GGGAATGTTT AAAAGAACTG 
GAGGAATCTC ATGACCAGCA TGCAGTATTA GTGCTACAGC CTGCTGTCGA GGCCTTCTTT 
TTGGTCCATG CCACAGAGCG GGAGAGCAAG CCTCCTGTCC GAGATACCCG TGAGAGCCAG 
CTGGCACACA TCAAGGATGA ACCTCCTCCA CTCTCCCCCG CCCCCTTAAC CCCAGCCACT 
CCATCCTCCC TTGACCCATT CTTCTCCAGG GAGCCCTCAT CTATGCACAT TTCCTCGAGC 
CTGCCCCCTG ACACACAGAA GTTCCTTCGC TTTGCAGAGA CTCACCGCAC TGTGTTAAAC 
CAGATACTAC GGCAGTCTAC CACCCATCTT GCTGATGGGC CTTTTGCTGT CTTGGTAGAT 
TATATCCGTG TCCTCGACTT TGATGTCAAG CGCAAATATT TCCGCCAAGA GCTGGAGCGT 
TTGGACGAAG GGCTCCGGAA AGAAGATATG GCAGTGCATG TCCGCCGTGA CCATGTATTT 
GAAGACTCCT ATCGTGAGCT GCATCGCAAA TCTCCTGAAG AAATGAAGAA TCGATTGTAT 
ATAGTGTTTG AAGGAGAAGA AGGGCAGGAT GCTGGGGGGC TCCTGCGAGA GTGGTACATG 
ATCATCTCTC GAGAGATGTT CAACCCTATG TATGCCTTGT TCCGTACCTC ACCTGGTGAC 
CGGGTCACCT ACACCATCAA TCCATCTTCC CACTGTAACC CCAACCACCT CAGCTACTTC 
AAGTTTGTTG GACGAATTGT AGCCAAAGCT GTATATGACA ACCGCCTCCT GGAGTGCTAC 
TTTACTAGGT CTTTCTACAA ACACATCTTG GGCAAATCTG TCAGGTATAC AGATATGGAG 
AGTGAAGATT ACCACTTCTA CCAGGGCCTA GTTTATCTGC TTGAAAATGA TGTCTCTACA 
TTAGGCTATG ACCTCACCTT CAGCACTGAG GTTCAAGAAT TTGGAGTATG TGAAGTTCGT 
GACCTCAAAC CCAATGGAGC CAACATCTTG GTAACAGAGG AGAACAAGAA GGAATATGTA 
CACCTGGTTT GCCAGATGAG AATGACAGGA GCCATCCGCA AACAGCTGGC AGCCTTCTTG 
GAGGGCTTCT ATGAAATCAT TCCAAAGCGC CTCATATCCA TCTTTACTGA GCAGGAATTA 
GAGCTGCTCA TATCAGGGCT GCCTACCATC GACATTGATG ATCTAAAATC TAACACTGAG 
TACCACAAGT ACCAATCCAA CTCTATTCAG ATCCAGTGGT TTTGGAGAGC ATTGCGTTCC 
TTTGACCAAG CAGACCGTGC CAAGTTCCTC CAGTTTGTCA CAGGTACTTC CAAGGTACCC 
CTACAAGGAT TTGCTGCCCT TGAAGGTATG AATGGCATTC AGAAGTTTCA GATTCATCGA 
GATGACAGAT CCACAGATCG CCTGCCTTCA GCTCACACAT GTTTTAATCA ACTGGACCTG 
CCTGCCTATG AGAGCTTTGA GAAGCTCCGC CACATGCTAC TATTGGCCAT CCAGGAATGC 
TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246158.1
CDS	1191..14534
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKADGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_011247856.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247856.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGAT 
GGAGAACAGG ATGGAACTGC TGGAAGCATG GATGCATCTG CCCAGGGTTT ATTAGAAGGA 
ATTGAACTAG ATGGTGACAC ACTGGCACCC ATGGAGACAG ATGAACCTTC TTCTTCAGAT 
TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCAA GAATTAAACA AATCAAGCCG 
TTGCTGTCAG CTTCTTCCAG ATTAGGCCGA GCACTTGCTG AGCTCTTTGG ACTCCTTGTT 
AAGCTTTGTG TGGGTTCTCC TGTCCGCCAA AGGAGGAGCC ATCATGCTGC CAGTACCACC 
ACTGCACCAA CACCTGCTGC TAGATCAACA GCTTCAGCCC TCACTAAGCT ACTCACAAAA 
GGCTTATCAT GGCAGCCCCC ACCATATACA CCCACTCCCC GCTTCAGGCT GACGTTCTTC 
ATCTGTTCAG TTGGATTCAC CTCTCCAATG CTCTTTGATG AGAGAAAGTA TCCTTACCAC 
CTTATGCTGC AGAAATTTCT CTGCTCTGGT GGTCACAATG CTCTTTTTGA AACTTTCAAC 
TGGGCTCTAT CCATGGGAGG AAAAGTTCCT GTTTCTGAGG GATTGGAACA TTCAGATCTA 
CCTGATGGTA CAGGAGAATT CCTAGATGCC TGGCTTATGC TTGTAGAAAA GATGGTAAAT 
CCCACCACGG TGCTTGAATC TCCACATTCG TTGCCTGCTA AATTGCCTGG AGGAGTCCAG 
AGCTTTCCAC AATTTAGTGC CCTTCGCTTC CTTGTAGTAA CTCAAAAAGC AGCCTTTACT 
TGTATCAAGA ACCTGTGGAA CCGGAAACCC CTTAAAGTAT ATGGGGGAAG AATGGCTGAG 
TCTATGCTGG CCATTTTGTG CCACATTCTC CGAGGGGAAC CTGTGATTCG AGAAAGACTG 
AGCAAAGAGA AGGAAGGATC TCGAGGAGAA GAAGAAGCAG GCCAGGAAGA AGGTGGCTCA 
CGCAGAGAAC CTCAAGTCAA CCAGCAACAG CTACAACAGC TCATGGACAT GGGTTTCACA 
AGAGAACATG CCATGGAGGC ACTGTTGAAT ACCAGTACGA TGGAACAAGC AACAGAGTAC 
CTTTTAACCC ACCCTCCTCC AATCATAGGA GGAGTTGTTC GGGATCTTAG CATGTCAGAA 
GAGGACCAGA TGATGAGAGC AATTGCTATG TCTCTGGGAC AGGATATTCC AATGGATCAA 
AGGGCAGAGT CACCTGAGGA AGTTGCTTGC CGGAAGGAGG AAGAAGAGCG TAAAGCTCGA 
GAAAAACAAG AAGAGGAAGA AGCTAAATGT CTAGAGAAGT TTCAAGATGC TGACCCCTTG 
GAACAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTAC CAGGCTGCTT CCATCTTCTT 
GATGAGTTGC CTGATACAGT GTACCGTGTT TGTGATCTGA TCATGACAGC TATCAAACGT 
AATGGAGCTG ACTATCGTGA TATGATTCTA AAGCAAGTTG TCAACCAGGT GTGGGAAGCT 
GCTGATGTAT TGATCAAAGC TGCTCTTCCT CTAACAACAA GTGACACAAA AACAGTTTCA 
GAGTGGATCA GTCAGATGGC TACCCTGCCA CAGGCCTCCA ATTTGGCTAC TAGAATCTTG 
CTTTTAACAC TACTTTTTGA GGAGTTGAAG TTACCTTGTG CCTGGGTGGT TGAATCAAGT 
GGCATTCTTA ATGTCCTAAT CAAACTCTTA GAAGTGGTTC AACCCTGCCT ACAGGCTGCC 
AAAGAGCAAA AGGAAGTCCA GACCCCAAAG TGGATCACCC CAGTGTTGCT CCTTATTGAT 
TTTTATGAAA AGACAGCCAT CTCATCAAAG AGAAGAGCCC AAATGACTAA GTACCTACAG 
TCCAACAGCA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GGCGTTGGTG TAGTTACAGT 
GCTAGCAACA ACAGCACCAT TGATTCTGCC TGGAAATCTG GAGAAACAAG TGTAAGATTC 
ACTGCTGGCC GACGAAGATA CACTGTCCAG TTCACTACAA TGGTGCAGGT TAATGAAGAA 
ACAGGAAACC GACGCCCTGT GATGCTGACT CTCCTCAGGG TGCCACGACT GAGTAAGAAC 
TCAAAAAGCA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA GGAGACCGAT 
ATCAAACATA AAGAAAATAA AGGCAATGAT ATCCCCTTAG CTCTAGAGAG CACAAACACT 
GAAAAAGAGG CAAGCCTGGA TGAAACAAAA ATTGGGGAGA TCCTAATCCA GGGCCTGACA 
GAAGATATGG TGACTGTTCT AATCCGTGCC TGTGTGAGCA TGCTGGGAGT CCCTGTGGAC 
CCAGACACTT TGCATGCCAC CCTTCGTCTC TGTTTAAGGC TCACCCGAGA CCACAAATAT 
GCCATGATGT TTGCAGAACT GAAGAGTACA CGAATGATCT TGAATTTGAC CCAGAGCTCA 
GGTTTCAATG GGTTTACCCC CTTAGTCACC CTTCTCTTAA GGCACATCAT TGAGGATCCT 
TGTACTCTTC GTCATACTAT GGAAAAGGTT GTTCGCTCGG CAGCCACAAG TGGAGCTGGT 
AGTACTACAT CTGGTGTTGT ATCTGGCAGC CTCGGTTCTC GGGAGATCAA CTATATCCTT 
AGAGTCCTAG GACCAGCTGC CTGCCGCAAT CCAGACATAT TCACAGAAGT AGCCAATTGC 
TGTATCCGCA TTGCCCTTCC AGCCCCTCGA GGCTCAGGAA CTGCTTCAGA TGATGAATTT 
GAGAATCTTA GAATTAAAGG TCCTAATGCT GTACAGCTGG TAAAGACAAC ACCTTTGAAG 
CCCTCATCTC TGCCTGTCAT TCCTGATACT ATTAAGGAAG TTATCTATGA TATGCTGAAT 
GCTTTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCTGGGGGA 
ACGACCCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ACGATGTATA CCAGCAGTAC 
CGGTCACTTA CTCGTCAGAG CAGTGACTTT GATACACAGT CAGGTTTTTC CTTAAATAGT 
CAGGTTTTTG CTGCAGATGG TGCCCCTGCT GAAACTTCCA CAACTGGAAC CTCCCAAGGA 
GAGGGAGCTT CAACTCCAGA GGAGACTCGA GAAGGGAAGA AAGATAAAGA AGGGGACCGT 
ACATCTGAGG AAGGCAAGCA GAAAAGCAAG GGCAGCAAAC CTTTAATGCC TACCTCTACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCA 
AACTACAGCT ATACCGTGGG CCAGTCTGAG CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTCCTGG ACCACCTCCT CCCACACACC CAGAATGCAG AAGACAAGGA TACACCTGCC 
CTGGCACGTC TATTCCTTGC AAGTCTGGCT GCTGCGGGGA GTGGCACAGA TGCTCAAGTG 
GCCCTAGTGA ATGAAGTGAA AGCAGCCCTG GGACGGGCGC TTGCTATGGC TGAGAGTACA 
GAGAAGCACG CCAGACTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAATCCTGC 
CCCTCCACCT CCAGCTTCTA CAGTAGTGCT ACAGCCAAGA CTCAGCATAA TGGCATGAAC 
AACATCATTC GACTGTTCCT AAAGAAGGGA CTAGTCAATG ACCTGGCCAG AGTTCCTCAC 
AGCCTAGACC TGTCCAGTCC CAATATGGCC AACACAGTCA ATGCTGCTCT AAAACCCTTG 
GAAACACTTT CCCGGATTGT AAATCAACCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCTCAA GGAGCTTCTC AGGACTCCAG CAGCCATCAG 
CAAGATCCAG GCGAGCCTGG GGAGGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCCGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAAAATGA ACTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AACATACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAAG GAGATGAAGA TGATGACGAC GATGGTTCTG AGATGGAATT GGATGAGGAT 
TACCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATTCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCACCC ATTGATGGTA CGTCATGCAG ATCACAGTTC CCTGACACTG 
GGCAGTGGTT CTTCCACAAC TCGTCTTACC CAGGGCATCG GTCGCAGTCA AAGGACCCTC 
AGACAGCTCA CTGCCAATAC AGGCCATACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCTAACCCTC CTCTTATACT CCAGAGGTTG CTTGGTCCCT CTGCAGCTGC TGACATTCTT 
CAGCTGAGCA GCAGCCTTCC ATTACAAAGC CGAGGCCGGG CCCGCCTCCT AGTGGGCAAT 
GATGACGTCC ATATCATCGC CAGGTCTGAT GACGAGCTGC TGGATGACTT TTTTCATGAC 
CAGAGTACAG CCACTAGCCA AGCAGGAACC CTGTCCAGCA TCCCAACAGC TCTAACCCGA 
TGGACAGAGG AATGCAAAGT TCTTGATGCT GAGAGTATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGCCCA TTGTCAATCA CCTGGAATTC TTGAGAGATG AGGAGCTAGA AGAGAGAAGG 
GAGAAACGCA GGAAACAATT GGCTGAGGAA GAAACAAAAA TAATAGACAA AGGCAAAGAA 
GACAAGGAGA ACAGAGATCA GAGTGCACAG TGTACTGTAA CTAAGACAAA TGACTCCACT 
GAACAGAATG TCTCAGATGG GACTCCTATG CCTGACAGCT ATCCAACAAC CCCCTCTTCA 
ACGGATGCAC CTACATCTGA GTCCAAGGAA ACACTTGGTA CCCTACAACC CTCCCAGCAA 
CAGCCAGCAC TTCCACCCCC ACCATCCTTA GGAGAGATTC CTCAGGAACT ACAGTCCCCT 
GCTGAAGAAG TGGCAAACTC TACACAGCTA TTGATGCCTA TAGAGTTGGA GGAATTGGGT 
CCCACAAGAC CAAGTGGAGA AGCAGAAACA ACTCAAATGG AATTGTCTCC AGCTCCCACC 
ATAACCTCTC TTTCCCCAGA GAGAGCTGAA GATTCAGACG CGTTAACAGC TGTCAGCAGT 
CAGCTGGAAG GTTCTCCCAT GGACACCAGC AGCCTGGCTT CCTGTACGTT AGAAGAGGCT 
GTGGGTGACA CTCCAGCAGC TGGCAGTTCT GAGCAGCCCA CAGCAGGTAG TTCCACTCCT 
GGGGATGCGC CATCAGTTGT GGCAGAAGTG CAAGGCAGAC CTGATGTATC AAGAGAATCC 
AACCAGCCAC CTGAGGACAG CTCCCCCCCT GCATCCTCGG AGAGTTCTTC CACTAGAGAT 
TCTGCCGTGG CCATCTCTGG AGCAGATTCT CGTGGAATTC TAGAAGAGCC ACTACCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCT GGTATCAGTC TCCCTGAAGG TGTGGATCCC 
TCCTTTCTGG CTGCTCTCCC TGATGATATC CGTCGGGAAG TCCTACAGAA CCAGCTGGGC 
ATACGTCCAC CAACCCGAAG TGCTCCCTCC TCAAATAGCT CAGCTCCTGC AGTAGTGGGG 
AACCCTGGTG TAACTGAAGT GAGCCCTGAG TTCCTGGCTG CCCTACCTCC AGCTATTCAA 
GAGGAAGTGC TAGCACAGCA GAGAGCTGAA CAGCAGCGAC GGGAACTGGC TCAGAATGCG 
AGCTCAGACA CCCCCATGGA CCCTGTGACT TTCATTCAGA CTTTGCCCTC AGATCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAAGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCTCTAAG ACGAGAACAA GAAGCCCGAC AGCGGCAGCT CATGCATGAG 
CGTCTTTTTG GACACAGTAG TACTTCTGCA CTCTCTGCCA TTCTCCGAAG CCCGGCTTTT 
ACCAGTCGCC TGAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT ACAGAGGGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAATAGGC CTTCTGGCAG TAATGTGGAC 
ACACTACTCC GCCTCCGAGG ACGACTCCTT CTAGACCATG AAGCCCTGTC TTGCCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ATCATGCCCA GACCCGCCAC TGGGTCATCC GCAGCCTCCT GTCCATTTTG 
CAGCGCAGCA GTGAAAGTGA GTTATGCATT GAGACACCTA AGCTCTCCAC AAGTGAAGAA 
AGGGGCAAGA AATCAAGCAA GAGCTGTGCA TCAAGCAGTC ATGAGAACCG TCCCCTGGAT 
CTGCTCCACA AGATGGAGTC CAAAAGTTCC AATCAGCTCT CCTGGCTCTC AGTATCAATG 
GATGCTGCTT TAGGCTGTAG GACTAACATA TTCCAGATTC AACGTTCTGG AGGGCGTAAA 
CATACAGAGA AGCATGCAAG CAGTGGTTCC ACTGTCCACA TCCACCCCCA GGCTGCTCCT 
GTTGTTTGCA GACATGTTTT GGATACACTC ATTCAGTTGG CCAAGGTGTT TCCCAGCCAC 
TTTACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAACG GGGCAGTAAG 
CAGGCCTGCA GCCCATGCTC CTCACAGTCC TCCAGCAGTG GCATCTGTAC AGACTTCTGG 
GACTTATTGG TAAAACTGGA CAACATGAAT GTTAGCCGAA AAGGCAAGAA CTCCGTGAAG 
TCAGTACCAG TGAGCTCTGG TGGTGAAGGG GAAACTTCCC CACACAGCCT TGAAGCCTCT 
CCACTGGGGC AGCTCATGAA CATGTTGTCA CATCCAGTCA TCCGCCGGAG TTCTCTCTTA 
ACTGAGAAAC TCCTGAGACT CCTCTCTCTC ATCTCAATTG CTCTCCCAGA AAACAAAGTA 
TCAGAAGTGC AAACTAACTC CAGCAACAGT GGTTCCTCCA CTGCTGCCAC CTCAAACACA 
TCTACTACTA CCACCACCAC CACTACTGCC ACTGCCCCCA CGCCCACACC CCCAGCTGCA 
ACTACCCCTG TCACTTCTGC TCCAGCTCTG GTTGCTGCCA CAGCTATTTC CACCATTACT 
GTAGCTGCTT CTACCACAGT GACTACCCCC ACAACTGCTA CCACTACTGT TTCAACTTCT 
ACTACTAAAG GCAGCAAATC TCCAGCAAAG GTTGGTGAAG GAGGCAGTGG TATAGACTTC 
AAAATGGTGT CCTCTGGCCT CACTGAAAAC CAGCTACAGC TCTCTGTAGA GGTGTTGACA 
TCCCACTCTT GTTCTGAAGA AGGCTTGGAA GATGCGGCCA ATGTGTTACT GCAGCTCTCT 
CGTGGGGACT CTGGTACCCG GGACACTGTT CTTAAGCTGC TACTAAATGG AGCCCGCCAT 
CTGGGTTATA CCCTCTGTAA ACAGATAGGT ACCCTACTCG CTGAACTACG GGAATATAAT 
CTGGAGCAGC AGCGGCGAGC CCAATGCGAG ACCCTCTCTC CTGATGGCCT ACCTGAGGAG 
CAACCACAGA CCACCAAACT GAAAGGCAAA ATGCAGAGCA GGTTTGACAT GGCTGAAAAT 
GTGGTAATTG TGGCATCTCA GAAGCGACCC TTGGGTGGCC GGGAGCTCCA GCTGCCTTCT 
ATGTCCATGT TGACATCCAA GACATCTACC CAGAAGTTCT TCTTGAGGGT ACTACAGGTC 
ATCATCCAGC TGCGGGATGA CACACGACGA GCTAACAAGA AAGCCAAGCA GACAGGCAGG 
CTAGGTTCCT CCGGTTTAGG CTCAGCTAGC AGCATCCAGG CAGCTGTTCG GCAGCTGGAG 
GCTGAGGCTG ATGCCATTAT ACAAATGGTA CGTGAGGGTC AAAGGGCGCG GAGACAGCAA 
CAAGCAGCAA CGTCGGAGTC TTCTAACCAG TCAGAGACAT CTGTCCGGAG AGAGGAATCG 
CCCATGGATG TGGACCAGCC ATCTCCTAGT GCTCAAGATA CTCAGTCCAT TGTAATCTCA 
GATGGAACAC CACAGGGAGA GAAGGAGAAA GAGGAGAAAC CACCTGAGTT ACCCCTGCTC 
AGTGAGCAAC TGAGCCTAGA TGAACTGTGG GACATGCTAG GGGAATGTTT AAAAGAACTG 
GAGGAATCTC ATGACCAGCA TGCAGTATTA GTGCTACAGC CTGCTGTCGA GGCCTTCTTT 
TTGGTCCATG CCACAGAGCG GGAGAGCAAG CCTCCTGTCC GAGATACCCG TGAGAGCCAG 
CTGGCACACA TCAAGGATGA ACCTCCTCCA CTCTCCCCCG CCCCCTTAAC CCCAGCCACT 
CCATCCTCCC TTGACCCATT CTTCTCCAGG GAGCCCTCAT CTATGCACAT TTCCTCGAGC 
CTGCCCCCTG ACACACAGAA GTTCCTTCGC TTTGCAGAGA CTCACCGCAC TGTGTTAAAC 
CAGATACTAC GGCAGTCTAC CACCCATCTT GCTGATGGGC CTTTTGCTGT CTTGGTAGAT 
TATATCCGTG TCCTCGACTT TGATGTCAAG CGCAAATATT TCCGCCAAGA GCTGGAGCGT 
TTGGACGAAG GGCTCCGGAA AGAAGATATG GCAGTGCATG TCCGCCGTGA CCATGTATTT 
GAAGACTCCT ATCGTGAGCT GCATCGCAAA TCTCCTGAAG AAATGAAGAA TCGATTGTAT 
ATAGTGTTTG AAGGAGAAGA AGGGCAGGAT GCTGGGGGGC TCCTGCGAGA GTGGTACATG 
ATCATCTCTC GAGAGATGTT CAACCCTATG TATGCCTTGT TCCGTACCTC ACCTGGTGAC 
CGGGTCACCT ACACCATCAA TCCATCTTCC CACTGTAACC CCAACCACCT CAGCTACTTC 
AAGTTTGTTG GACGAATTGT AGCCAAAGCT GTATATGACA ACCGCCTCCT GGAGTGCTAC 
TTTACTAGGT CTTTCTACAA ACACATCTTG GGCAAATCTG TCAGGTATAC AGATATGGAG 
AGTGAAGATT ACCACTTCTA CCAGGGCCTA GTTTATCTGC TTGAAAATGA TGTCTCTACA 
TTAGGCTATG ACCTCACCTT CAGCACTGAG GTTCAAGAAT TTGGAGTATG TGAAGTTCGT 
GACCTCAAAC CCAATGGAGC CAACATCTTG GTAACAGAGG AGAACAAGAA GGAATATGTA 
CACCTGGTTT GCCAGATGAG AATGACAGGA GCCATCCGCA AACAGCTGGC AGCCTTCTTG 
GAGGGCTTCT ATGAAATCAT TCCAAAGCGC CTCATATCCA TCTTTACTGA GCAGGAATTA 
GAGCTGCTCA TATCAGGGCT GCCTACCATC GACATTGATG ATCTAAAATC TAACACTGAG 
TACCACAAGT ACCAATCCAA CTCTATTCAG ATCCAGTGGT TTTGGAGAGC ATTGCGTTCC 
TTTGACCAAG CAGACCGTGC CAAGTTCCTC CAGTTTGTCA CAGGTACTTC CAAGGTACCC 
CTACAAGGAT TTGCTGCCCT TGAAGGTATG AATGGCATTC AGAAGTTTCA GATTCATCGA 
GATGACAGAT CCACAGATCG CCTGCCTTCA GCTCACACAT GTTTTAATCA ACTGGACCTG 
CCTGCCTATG AGAGCTTTGA GAAGCTCCGC CACATGCTAC TATTGGCCAT CCAGGAATGC 
TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251447.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107307.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X23, mRNA.

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CloneID	OMu69813
Clone ID Related Accession (Same CDS sequence)	XM_030251447.1 , XM_011247857.1
Accession Version	XM_030251447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGTCGGAG 
TCTTCTAACC AGTCAGAGAC ATCTGTCCGG AGAGAGGAAT CGCCCATGGA TGTGGACCAG 
CCATCTCCTA GTGCTCAAGA TACTCAGTCC ATTGTAATCT CAGATGGAAC ACCACAGGGA 
GAGAAGGAGA AAGAGGAGAA ACCACCTGAG TTACCCCTGC TCAGTGAGCA ACTGAGCCTA 
GATGAACTGT GGGACATGCT AGGGGAATGT TTAAAAGAAC TGGAGGAATC TCATGACCAG 
CATGCAGTAT TAGTGCTACA GCCTGCTGTC GAGGCCTTCT TTTTGGTCCA TGCCACAGAG 
CGGGAGAGCA AGCCTCCTGT CCGAGATACC CGTGAGAGCC AGCTGGCACA CATCAAGGAT 
GAACCTCCTC CACTCTCCCC CGCCCCCTTA ACCCCAGCCA CTCCATCCTC CCTTGACCCA 
TTCTTCTCCA GGGAGCCCTC ATCTATGCAC ATTTCCTCGA GCCTGCCCCC TGACACACAG 
AAGTTCCTTC GCTTTGCAGA GACTCACCGC ACTGTGTTAA ACCAGATACT ACGGCAGTCT 
ACCACCCATC TTGCTGATGG GCCTTTTGCT GTCTTGGTAG ATTATATCCG TGTCCTCGAC 
TTTGATGTCA AGCGCAAATA TTTCCGCCAA GAGCTGGAGC GTTTGGACGA AGGGCTCCGG 
AAAGAAGATA TGGCAGTGCA TGTCCGCCGT GACCATGTAT TTGAAGACTC CTATCGTGAG 
CTGCATCGCA AATCTCCTGA AGAAATGAAG AATCGATTGT ATATAGTGTT TGAAGGAGAA 
GAAGGGCAGG ATGCTGGGGG GCTCCTGCGA GAGTGGTACA TGATCATCTC TCGAGAGATG 
TTCAACCCTA TGTATGCCTT GTTCCGTACC TCACCTGGTG ACCGGGTCAC CTACACCATC 
AATCCATCTT CCCACTGTAA CCCCAACCAC CTCAGCTACT TCAAGTTTGT TGGACGAATT 
GTAGCCAAAG CTGTATATGA CAACCGCCTC CTGGAGTGCT ACTTTACTAG GTCTTTCTAC 
AAACACATCT TGGGCAAATC TGTCAGGTAT ACAGATATGG AGAGTGAAGA TTACCACTTC 
TACCAGGGCC TAGTTTATCT GCTTGAAAAT GATGTCTCTA CATTAGGCTA TGACCTCACC 
TTCAGCACTG AGGTTCAAGA ATTTGGAGTA TGTGAAGTTC GTGACCTCAA ACCCAATGGA 
GCCAACATCT TGGTAACAGA GGAGAACAAG AAGGAATATG TACACCTGGT TTGCCAGATG 
AGAATGACAG GAGCCATCCG CAAACAGCTG GCAGCCTTCT TGGAGGGCTT CTATGAAATC 
ATTCCAAAGC GCCTCATATC CATCTTTACT GAGCAGGAAT TAGAGCTGCT CATATCAGGG 
CTGCCTACCA TCGACATTGA TGATCTAAAA TCTAACACTG AGTACCACAA GTACCAATCC 
AACTCTATTC AGATCCAGTG GTTTTGGAGA GCATTGCGTT CCTTTGACCA AGCAGACCGT 
GCCAAGTTCC TCCAGTTTGT CACAGGTACT TCCAAGGTAC CCCTACAAGG ATTTGCTGCC 
CTTGAAGGTA TGAATGGCAT TCAGAAGTTT CAGATTCATC GAGATGACAG ATCCACAGAT 
CGCCTGCCTT CAGCTCACAC ATGTTTTAAT CAACTGGACC TGCCTGCCTA TGAGAGCTTT 
GAGAAGCTCC GCCACATGCT ACTATTGGCC ATCCAGGAAT GCTCTGAAGG CTTTGGGCTG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107307.1
CDS	1856..15181
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251447.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251447.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGTCGGAG 
TCTTCTAACC AGTCAGAGAC ATCTGTCCGG AGAGAGGAAT CGCCCATGGA TGTGGACCAG 
CCATCTCCTA GTGCTCAAGA TACTCAGTCC ATTGTAATCT CAGATGGAAC ACCACAGGGA 
GAGAAGGAGA AAGAGGAGAA ACCACCTGAG TTACCCCTGC TCAGTGAGCA ACTGAGCCTA 
GATGAACTGT GGGACATGCT AGGGGAATGT TTAAAAGAAC TGGAGGAATC TCATGACCAG 
CATGCAGTAT TAGTGCTACA GCCTGCTGTC GAGGCCTTCT TTTTGGTCCA TGCCACAGAG 
CGGGAGAGCA AGCCTCCTGT CCGAGATACC CGTGAGAGCC AGCTGGCACA CATCAAGGAT 
GAACCTCCTC CACTCTCCCC CGCCCCCTTA ACCCCAGCCA CTCCATCCTC CCTTGACCCA 
TTCTTCTCCA GGGAGCCCTC ATCTATGCAC ATTTCCTCGA GCCTGCCCCC TGACACACAG 
AAGTTCCTTC GCTTTGCAGA GACTCACCGC ACTGTGTTAA ACCAGATACT ACGGCAGTCT 
ACCACCCATC TTGCTGATGG GCCTTTTGCT GTCTTGGTAG ATTATATCCG TGTCCTCGAC 
TTTGATGTCA AGCGCAAATA TTTCCGCCAA GAGCTGGAGC GTTTGGACGA AGGGCTCCGG 
AAAGAAGATA TGGCAGTGCA TGTCCGCCGT GACCATGTAT TTGAAGACTC CTATCGTGAG 
CTGCATCGCA AATCTCCTGA AGAAATGAAG AATCGATTGT ATATAGTGTT TGAAGGAGAA 
GAAGGGCAGG ATGCTGGGGG GCTCCTGCGA GAGTGGTACA TGATCATCTC TCGAGAGATG 
TTCAACCCTA TGTATGCCTT GTTCCGTACC TCACCTGGTG ACCGGGTCAC CTACACCATC 
AATCCATCTT CCCACTGTAA CCCCAACCAC CTCAGCTACT TCAAGTTTGT TGGACGAATT 
GTAGCCAAAG CTGTATATGA CAACCGCCTC CTGGAGTGCT ACTTTACTAG GTCTTTCTAC 
AAACACATCT TGGGCAAATC TGTCAGGTAT ACAGATATGG AGAGTGAAGA TTACCACTTC 
TACCAGGGCC TAGTTTATCT GCTTGAAAAT GATGTCTCTA CATTAGGCTA TGACCTCACC 
TTCAGCACTG AGGTTCAAGA ATTTGGAGTA TGTGAAGTTC GTGACCTCAA ACCCAATGGA 
GCCAACATCT TGGTAACAGA GGAGAACAAG AAGGAATATG TACACCTGGT TTGCCAGATG 
AGAATGACAG GAGCCATCCG CAAACAGCTG GCAGCCTTCT TGGAGGGCTT CTATGAAATC 
ATTCCAAAGC GCCTCATATC CATCTTTACT GAGCAGGAAT TAGAGCTGCT CATATCAGGG 
CTGCCTACCA TCGACATTGA TGATCTAAAA TCTAACACTG AGTACCACAA GTACCAATCC 
AACTCTATTC AGATCCAGTG GTTTTGGAGA GCATTGCGTT CCTTTGACCA AGCAGACCGT 
GCCAAGTTCC TCCAGTTTGT CACAGGTACT TCCAAGGTAC CCCTACAAGG ATTTGCTGCC 
CTTGAAGGTA TGAATGGCAT TCAGAAGTTT CAGATTCATC GAGATGACAG ATCCACAGAT 
CGCCTGCCTT CAGCTCACAC ATGTTTTAAT CAACTGGACC TGCCTGCCTA TGAGAGCTTT 
GAGAAGCTCC GCCACATGCT ACTATTGGCC ATCCAGGAAT GCTCTGAAGG CTTTGGGCTG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_011247857.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246159.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X16, mRNA.

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CloneID	OMu69813
Clone ID Related Accession (Same CDS sequence)	XM_030251447.1 , XM_011247857.1
Accession Version	XM_011247857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGTCGGAG 
TCTTCTAACC AGTCAGAGAC ATCTGTCCGG AGAGAGGAAT CGCCCATGGA TGTGGACCAG 
CCATCTCCTA GTGCTCAAGA TACTCAGTCC ATTGTAATCT CAGATGGAAC ACCACAGGGA 
GAGAAGGAGA AAGAGGAGAA ACCACCTGAG TTACCCCTGC TCAGTGAGCA ACTGAGCCTA 
GATGAACTGT GGGACATGCT AGGGGAATGT TTAAAAGAAC TGGAGGAATC TCATGACCAG 
CATGCAGTAT TAGTGCTACA GCCTGCTGTC GAGGCCTTCT TTTTGGTCCA TGCCACAGAG 
CGGGAGAGCA AGCCTCCTGT CCGAGATACC CGTGAGAGCC AGCTGGCACA CATCAAGGAT 
GAACCTCCTC CACTCTCCCC CGCCCCCTTA ACCCCAGCCA CTCCATCCTC CCTTGACCCA 
TTCTTCTCCA GGGAGCCCTC ATCTATGCAC ATTTCCTCGA GCCTGCCCCC TGACACACAG 
AAGTTCCTTC GCTTTGCAGA GACTCACCGC ACTGTGTTAA ACCAGATACT ACGGCAGTCT 
ACCACCCATC TTGCTGATGG GCCTTTTGCT GTCTTGGTAG ATTATATCCG TGTCCTCGAC 
TTTGATGTCA AGCGCAAATA TTTCCGCCAA GAGCTGGAGC GTTTGGACGA AGGGCTCCGG 
AAAGAAGATA TGGCAGTGCA TGTCCGCCGT GACCATGTAT TTGAAGACTC CTATCGTGAG 
CTGCATCGCA AATCTCCTGA AGAAATGAAG AATCGATTGT ATATAGTGTT TGAAGGAGAA 
GAAGGGCAGG ATGCTGGGGG GCTCCTGCGA GAGTGGTACA TGATCATCTC TCGAGAGATG 
TTCAACCCTA TGTATGCCTT GTTCCGTACC TCACCTGGTG ACCGGGTCAC CTACACCATC 
AATCCATCTT CCCACTGTAA CCCCAACCAC CTCAGCTACT TCAAGTTTGT TGGACGAATT 
GTAGCCAAAG CTGTATATGA CAACCGCCTC CTGGAGTGCT ACTTTACTAG GTCTTTCTAC 
AAACACATCT TGGGCAAATC TGTCAGGTAT ACAGATATGG AGAGTGAAGA TTACCACTTC 
TACCAGGGCC TAGTTTATCT GCTTGAAAAT GATGTCTCTA CATTAGGCTA TGACCTCACC 
TTCAGCACTG AGGTTCAAGA ATTTGGAGTA TGTGAAGTTC GTGACCTCAA ACCCAATGGA 
GCCAACATCT TGGTAACAGA GGAGAACAAG AAGGAATATG TACACCTGGT TTGCCAGATG 
AGAATGACAG GAGCCATCCG CAAACAGCTG GCAGCCTTCT TGGAGGGCTT CTATGAAATC 
ATTCCAAAGC GCCTCATATC CATCTTTACT GAGCAGGAAT TAGAGCTGCT CATATCAGGG 
CTGCCTACCA TCGACATTGA TGATCTAAAA TCTAACACTG AGTACCACAA GTACCAATCC 
AACTCTATTC AGATCCAGTG GTTTTGGAGA GCATTGCGTT CCTTTGACCA AGCAGACCGT 
GCCAAGTTCC TCCAGTTTGT CACAGGTACT TCCAAGGTAC CCCTACAAGG ATTTGCTGCC 
CTTGAAGGTA TGAATGGCAT TCAGAAGTTT CAGATTCATC GAGATGACAG ATCCACAGAT 
CGCCTGCCTT CAGCTCACAC ATGTTTTAAT CAACTGGACC TGCCTGCCTA TGAGAGCTTT 
GAGAAGCTCC GCCACATGCT ACTATTGGCC ATCCAGGAAT GCTCTGAAGG CTTTGGGCTG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246159.1
CDS	1191..14516
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_011247857.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247857.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGTGAGCAGA AGTGGAGAGG ATGAATCACA AGAGGACGTG 
CTGATGGATG AAGCTCCTTC CAACCTCAGC CAAGCTTCCA CCTTGCAGGC CAACCGAGAA 
GATTCCATGA ATATCCTGGA CCCTGAGGAT GAGGAGGAAC ATACTCAGGA AGAGGACAGC 
AGTGGCAGTA ACGAGGATGA GGATGATAGT CAGGATGAAG AGGAGGAGGA GGAGGAAGAT 
GAGGAAGATG ATCAGGAGGA TGATGAAGGT GAAGAAGGAG ATGAAGATGA TGACGACGAT 
GGTTCTGAGA TGGAATTGGA TGAGGATTAC CCTGATATGA ACGCTTCTCC CTTGGTCCGA 
TTTGAGCGCT TTGACCGGGA GGATGATCTC ATCATTGAGT TTGACAACAT GTTCTCCAGT 
GCTACAGACA TTCCCCCATC CCCAGGAAAT ATCCCTACCA CCCACCCATT GATGGTACGT 
CATGCAGATC ACAGTTCCCT GACACTGGGC AGTGGTTCTT CCACAACTCG TCTTACCCAG 
GGCATCGGTC GCAGTCAAAG GACCCTCAGA CAGCTCACTG CCAATACAGG CCATACCATT 
CATGTTCACT ACCCTGGGAA TCGCCAGCCT AACCCTCCTC TTATACTCCA GAGGTTGCTT 
GGTCCCTCTG CAGCTGCTGA CATTCTTCAG CTGAGCAGCA GCCTTCCATT ACAAAGCCGA 
GGCCGGGCCC GCCTCCTAGT GGGCAATGAT GACGTCCATA TCATCGCCAG GTCTGATGAC 
GAGCTGCTGG ATGACTTTTT TCATGACCAG AGTACAGCCA CTAGCCAAGC AGGAACCCTG 
TCCAGCATCC CAACAGCTCT AACCCGATGG ACAGAGGAAT GCAAAGTTCT TGATGCTGAG 
AGTATGCATG ACTGTGTTTC AGTGGTTAAA GTGCCCATTG TCAATCACCT GGAATTCTTG 
AGAGATGAGG AGCTAGAAGA GAGAAGGGAG AAACGCAGGA AACAATTGGC TGAGGAAGAA 
ACAAAAATAA TAGACAAAGG CAAAGAAGAC AAGGAGAACA GAGATCAGAG TGCACAGTGT 
ACTGTAACTA AGACAAATGA CTCCACTGAA CAGAATGTCT CAGATGGGAC TCCTATGCCT 
GACAGCTATC CAACAACCCC CTCTTCAACG GATGCACCTA CATCTGAGTC CAAGGAAACA 
CTTGGTACCC TACAACCCTC CCAGCAACAG CCAGCACTTC CACCCCCACC ATCCTTAGGA 
GAGATTCCTC AGGAACTACA GTCCCCTGCT GAAGAAGTGG CAAACTCTAC ACAGCTATTG 
ATGCCTATAG AGTTGGAGGA ATTGGGTCCC ACAAGACCAA GTGGAGAAGC AGAAACAACT 
CAAATGGAAT TGTCTCCAGC TCCCACCATA ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT 
TCAGACGCGT TAACAGCTGT CAGCAGTCAG CTGGAAGGTT CTCCCATGGA CACCAGCAGC 
CTGGCTTCCT GTACGTTAGA AGAGGCTGTG GGTGACACTC CAGCAGCTGG CAGTTCTGAG 
CAGCCCACAG CAGGTAGTTC CACTCCTGGG GATGCGCCAT CAGTTGTGGC AGAAGTGCAA 
GGCAGACCTG ATGTATCAAG AGAATCCAAC CAGCCACCTG AGGACAGCTC CCCCCCTGCA 
TCCTCGGAGA GTTCTTCCAC TAGAGATTCT GCCGTGGCCA TCTCTGGAGC AGATTCTCGT 
GGAATTCTAG AAGAGCCACT ACCTTCAACA AGCAGTGAAG AAGAAGATCC CCTTGCTGGT 
ATCAGTCTCC CTGAAGGTGT GGATCCCTCC TTTCTGGCTG CTCTCCCTGA TGATATCCGT 
CGGGAAGTCC TACAGAACCA GCTGGGCATA CGTCCACCAA CCCGAAGTGC TCCCTCCTCA 
AATAGCTCAG CTCCTGCAGT AGTGGGGAAC CCTGGTGTAA CTGAAGTGAG CCCTGAGTTC 
CTGGCTGCCC TACCTCCAGC TATTCAAGAG GAAGTGCTAG CACAGCAGAG AGCTGAACAG 
CAGCGACGGG AACTGGCTCA GAATGCGAGC TCAGACACCC CCATGGACCC TGTGACTTTC 
ATTCAGACTT TGCCCTCAGA TCTGCGCCGT AGTGTCCTAG AGGATATGGA AGACAGTGTG 
TTAGCTGTGA TGCCACCTGA CATTGCAGCT GAGGCTCAAG CTCTAAGACG AGAACAAGAA 
GCCCGACAGC GGCAGCTCAT GCATGAGCGT CTTTTTGGAC ACAGTAGTAC TTCTGCACTC 
TCTGCCATTC TCCGAAGCCC GGCTTTTACC AGTCGCCTGA GTGGCAACCG TGGGGTCCAG 
TATACTCGCC TTGCTGTACA GAGGGGTGGC ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT 
AATAGGCCTT CTGGCAGTAA TGTGGACACA CTACTCCGCC TCCGAGGACG ACTCCTTCTA 
GACCATGAAG CCCTGTCTTG CCTCTTGGTC CTACTTTTTG TGGATGAGCC AAAGCTCAAT 
ACTAGCCGTC TACACCGAGT ACTGAGAAAT CTCTGCTATC ATGCCCAGAC CCGCCACTGG 
GTCATCCGCA GCCTCCTGTC CATTTTGCAG CGCAGCAGTG AAAGTGAGTT ATGCATTGAG 
ACACCTAAGC TCTCCACAAG TGAAGAAAGG GGCAAGAAAT CAAGCAAGAG CTGTGCATCA 
AGCAGTCATG AGAACCGTCC CCTGGATCTG CTCCACAAGA TGGAGTCCAA AAGTTCCAAT 
CAGCTCTCCT GGCTCTCAGT ATCAATGGAT GCTGCTTTAG GCTGTAGGAC TAACATATTC 
CAGATTCAAC GTTCTGGAGG GCGTAAACAT ACAGAGAAGC ATGCAAGCAG TGGTTCCACT 
GTCCACATCC ACCCCCAGGC TGCTCCTGTT GTTTGCAGAC ATGTTTTGGA TACACTCATT 
CAGTTGGCCA AGGTGTTTCC CAGCCACTTT ACACAGCAGC GGACCAAAGA AACAAACTGT 
GAGAGTGATC GGGAACGGGG CAGTAAGCAG GCCTGCAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TCTGTACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTT 
AGCCGAAAAG GCAAGAACTC CGTGAAGTCA GTACCAGTGA GCTCTGGTGG TGAAGGGGAA 
ACTTCCCCAC ACAGCCTTGA AGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAT 
CCAGTCATCC GCCGGAGTTC TCTCTTAACT GAGAAACTCC TGAGACTCCT CTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAAGTATCA GAAGTGCAAA CTAACTCCAG CAACAGTGGT 
TCCTCCACTG CTGCCACCTC AAACACATCT ACTACTACCA CCACCACCAC TACTGCCACT 
GCCCCCACGC CCACACCCCC AGCTGCAACT ACCCCTGTCA CTTCTGCTCC AGCTCTGGTT 
GCTGCCACAG CTATTTCCAC CATTACTGTA GCTGCTTCTA CCACAGTGAC TACCCCCACA 
ACTGCTACCA CTACTGTTTC AACTTCTACT ACTAAAGGCA GCAAATCTCC AGCAAAGGTT 
GGTGAAGGAG GCAGTGGTAT AGACTTCAAA ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAAGAAGG CTTGGAAGAT 
GCGGCCAATG TGTTACTGCA GCTCTCTCGT GGGGACTCTG GTACCCGGGA CACTGTTCTT 
AAGCTGCTAC TAAATGGAGC CCGCCATCTG GGTTATACCC TCTGTAAACA GATAGGTACC 
CTACTCGCTG AACTACGGGA ATATAATCTG GAGCAGCAGC GGCGAGCCCA ATGCGAGACC 
CTCTCTCCTG ATGGCCTACC TGAGGAGCAA CCACAGACCA CCAAACTGAA AGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAAAATGTG GTAATTGTGG CATCTCAGAA GCGACCCTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTGC GGGATGACAC ACGACGAGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGTCGGAG 
TCTTCTAACC AGTCAGAGAC ATCTGTCCGG AGAGAGGAAT CGCCCATGGA TGTGGACCAG 
CCATCTCCTA GTGCTCAAGA TACTCAGTCC ATTGTAATCT CAGATGGAAC ACCACAGGGA 
GAGAAGGAGA AAGAGGAGAA ACCACCTGAG TTACCCCTGC TCAGTGAGCA ACTGAGCCTA 
GATGAACTGT GGGACATGCT AGGGGAATGT TTAAAAGAAC TGGAGGAATC TCATGACCAG 
CATGCAGTAT TAGTGCTACA GCCTGCTGTC GAGGCCTTCT TTTTGGTCCA TGCCACAGAG 
CGGGAGAGCA AGCCTCCTGT CCGAGATACC CGTGAGAGCC AGCTGGCACA CATCAAGGAT 
GAACCTCCTC CACTCTCCCC CGCCCCCTTA ACCCCAGCCA CTCCATCCTC CCTTGACCCA 
TTCTTCTCCA GGGAGCCCTC ATCTATGCAC ATTTCCTCGA GCCTGCCCCC TGACACACAG 
AAGTTCCTTC GCTTTGCAGA GACTCACCGC ACTGTGTTAA ACCAGATACT ACGGCAGTCT 
ACCACCCATC TTGCTGATGG GCCTTTTGCT GTCTTGGTAG ATTATATCCG TGTCCTCGAC 
TTTGATGTCA AGCGCAAATA TTTCCGCCAA GAGCTGGAGC GTTTGGACGA AGGGCTCCGG 
AAAGAAGATA TGGCAGTGCA TGTCCGCCGT GACCATGTAT TTGAAGACTC CTATCGTGAG 
CTGCATCGCA AATCTCCTGA AGAAATGAAG AATCGATTGT ATATAGTGTT TGAAGGAGAA 
GAAGGGCAGG ATGCTGGGGG GCTCCTGCGA GAGTGGTACA TGATCATCTC TCGAGAGATG 
TTCAACCCTA TGTATGCCTT GTTCCGTACC TCACCTGGTG ACCGGGTCAC CTACACCATC 
AATCCATCTT CCCACTGTAA CCCCAACCAC CTCAGCTACT TCAAGTTTGT TGGACGAATT 
GTAGCCAAAG CTGTATATGA CAACCGCCTC CTGGAGTGCT ACTTTACTAG GTCTTTCTAC 
AAACACATCT TGGGCAAATC TGTCAGGTAT ACAGATATGG AGAGTGAAGA TTACCACTTC 
TACCAGGGCC TAGTTTATCT GCTTGAAAAT GATGTCTCTA CATTAGGCTA TGACCTCACC 
TTCAGCACTG AGGTTCAAGA ATTTGGAGTA TGTGAAGTTC GTGACCTCAA ACCCAATGGA 
GCCAACATCT TGGTAACAGA GGAGAACAAG AAGGAATATG TACACCTGGT TTGCCAGATG 
AGAATGACAG GAGCCATCCG CAAACAGCTG GCAGCCTTCT TGGAGGGCTT CTATGAAATC 
ATTCCAAAGC GCCTCATATC CATCTTTACT GAGCAGGAAT TAGAGCTGCT CATATCAGGG 
CTGCCTACCA TCGACATTGA TGATCTAAAA TCTAACACTG AGTACCACAA GTACCAATCC 
AACTCTATTC AGATCCAGTG GTTTTGGAGA GCATTGCGTT CCTTTGACCA AGCAGACCGT 
GCCAAGTTCC TCCAGTTTGT CACAGGTACT TCCAAGGTAC CCCTACAAGG ATTTGCTGCC 
CTTGAAGGTA TGAATGGCAT TCAGAAGTTT CAGATTCATC GAGATGACAG ATCCACAGAT 
CGCCTGCCTT CAGCTCACAC ATGTTTTAAT CAACTGGACC TGCCTGCCTA TGAGAGCTTT 
GAGAAGCTCC GCCACATGCT ACTATTGGCC ATCCAGGAAT GCTCTGAAGG CTTTGGGCTG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_011247859.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246161.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X20, mRNA.

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CloneID	OMu69814
Clone ID Related Accession (Same CDS sequence)	XM_011247859.1
Accession Version	XM_011247859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGAGAAC AGGATGGAAC TGCTGGAAGC 
ATGGATGCAT CTGCCCAGGG TTTATTAGAA GGAATTGAAC TAGATGGTGA CACACTGGCA 
CCCATGGAGA CAGATGAACC TTCTTCTTCA GATTCTAAGG GCAAATCTAA AATCACACCT 
GCCATGGCTG CAAGAATTAA ACAAATCAAG CCGTTGCTGT CAGCTTCTTC CAGATTAGGC 
CGAGCACTTG CTGAGCTCTT TGGACTCCTT GTTAAGCTTT GTGTGGGTTC TCCTGTCCGC 
CAAAGGAGGA GCCATCATGC TGCCAGTACC ACCACTGCAC CAACACCTGC TGCTAGATCA 
ACAGCTTCAG CCCTCACTAA GCTACTCACA AAAGGCTTAT CATGGCAGCC CCCACCATAT 
ACACCCACTC CCCGCTTCAG GCTGACGTTC TTCATCTGTT CAGTTGGATT CACCTCTCCA 
ATGCTCTTTG ATGAGAGAAA GTATCCTTAC CACCTTATGC TGCAGAAATT TCTCTGCTCT 
GGTGGTCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TATCCATGGG AGGAAAAGTT 
CCTGTTTCTG AGGGATTGGA ACATTCAGAT CTACCTGATG GTACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTTGTAGA AAAGATGGTA AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGTTGCCTG CTAAATTGCC TGGAGGAGTC CAGAGCTTTC CACAATTTAG TGCCCTTCGC 
TTCCTTGTAG TAACTCAAAA AGCAGCCTTT ACTTGTATCA AGAACCTGTG GAACCGGAAA 
CCCCTTAAAG TATATGGGGG AAGAATGGCT GAGTCTATGC TGGCCATTTT GTGCCACATT 
CTCCGAGGGG AACCTGTGAT TCGAGAAAGA CTGAGCAAAG AGAAGGAAGG ATCTCGAGGA 
GAAGAAGAAG CAGGCCAGGA AGAAGGTGGC TCACGCAGAG AACCTCAAGT CAACCAGCAA 
CAGCTACAAC AGCTCATGGA CATGGGTTTC ACAAGAGAAC ATGCCATGGA GGCACTGTTG 
AATACCAGTA CGATGGAACA AGCAACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATA 
GGAGGAGTTG TTCGGGATCT TAGCATGTCA GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAAGA GCGTAAAGCT CGAGAAAAAC AAGAAGAGGA AGAAGCTAAA 
TGTCTAGAGA AGTTTCAAGA TGCTGACCCC TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACAATGT TACCAGGCTG CTTCCATCTT CTTGATGAGT TGCCTGATAC AGTGTACCGT 
GTTTGTGATC TGATCATGAC AGCTATCAAA CGTAATGGAG CTGACTATCG TGATATGATT 
CTAAAGCAAG TTGTCAACCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCTCTAACAA CAAGTGACAC AAAAACAGTT TCAGAGTGGA TCAGTCAGAT GGCTACCCTG 
CCACAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CACTACTTTT TGAGGAGTTG 
AAGTTACCTT GTGCCTGGGT GGTTGAATCA AGTGGCATTC TTAATGTCCT AATCAAACTC 
TTAGAAGTGG TTCAACCCTG CCTACAGGCT GCCAAAGAGC AAAAGGAAGT CCAGACCCCA 
AAGTGGATCA CCCCAGTGTT GCTCCTTATT GATTTTTATG AAAAGACAGC CATCTCATCA 
AAGAGAAGAG CCCAAATGAC TAAGTACCTA CAGTCCAACA GCAACAACTG GCGCTGGTTT 
GATGACCGCT CTGGGCGTTG GTGTAGTTAC AGTGCTAGCA ACAACAGCAC CATTGATTCT 
GCCTGGAAAT CTGGAGAAAC AAGTGTAAGA TTCACTGCTG GCCGACGAAG ATACACTGTC 
CAGTTCACTA CAATGGTGCA GGTTAATGAA GAAACAGGAA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTGCCACG ACTGAGTAAG AACTCAAAAA GCAGCAATGG ACAGGAACTA 
GAGAAGACAC TGGAAGAAAG CAAGGAGACC GATATCAAAC ATAAAGAAAA TAAAGGCAAT 
GATATCCCCT TAGCTCTAGA GAGCACAAAC ACTGAAAAAG AGGCAAGCCT GGATGAAACA 
AAAATTGGGG AGATCCTAAT CCAGGGCCTG ACAGAAGATA TGGTGACTGT TCTAATCCGT 
GCCTGTGTGA GCATGCTGGG AGTCCCTGTG GACCCAGACA CTTTGCATGC CACCCTTCGT 
CTCTGTTTAA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACACGAATGA TCTTGAATTT GACCCAGAGC TCAGGTTTCA ATGGGTTTAC CCCCTTAGTC 
ACCCTTCTCT TAAGGCACAT CATTGAGGAT CCTTGTACTC TTCGTCATAC TATGGAAAAG 
GTTGTTCGCT CGGCAGCCAC AAGTGGAGCT GGTAGTACTA CATCTGGTGT TGTATCTGGC 
AGCCTCGGTT CTCGGGAGAT CAACTATATC CTTAGAGTCC TAGGACCAGC TGCCTGCCGC 
AATCCAGACA TATTCACAGA AGTAGCCAAT TGCTGTATCC GCATTGCCCT TCCAGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGTCCTAAT 
GCTGTACAGC TGGTAAAGAC AACACCTTTG AAGCCCTCAT CTCTGCCTGT CATTCCTGAT 
ACTATTAAGG AAGTTATCTA TGATATGCTG AATGCTTTGG CTGCATACCA TGCTCCAGAG 
GAAGACAAAT CTGATCCTAA ACCTGGGGGA ACGACCCAAG AGGTTGGCCA GCTCCTGCAA 
GACATGGGTG ACGATGTATA CCAGCAGTAC CGGTCACTTA CTCGTCAGAG CAGTGACTTT 
GATACACAGT CAGGTTTTTC CTTAAATAGT CAGGTTTTTG CTGCAGATGG TGCCCCTGCT 
GAAACTTCCA CAACTGGAAC CTCCCAAGGA GAGGGAGCTT CAACTCCAGA GGAGACTCGA 
GAAGGGAAGA AAGATAAAGA AGGGGACCGT ACATCTGAGG AAGGCAAGCA GAAAAGCAAG 
GGCAGCAAAC CTTTAATGCC TACCTCTACT ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG 
TCCTATGTTG GTATTGCTAC CCTGATTGCA AACTACAGCT ATACCGTGGG CCAGTCTGAG 
CTGATCAAAG AGGACTGCAG TGTGCTAGCT TTTGTCCTGG ACCACCTCCT CCCACACACC 
CAGAATGCAG AAGACAAGGA TACACCTGCC CTGGCACGTC TATTCCTTGC AAGTCTGGCT 
GCTGCGGGGA GTGGCACAGA TGCTCAAGTG GCCCTAGTGA ATGAAGTGAA AGCAGCCCTG 
GGACGGGCGC TTGCTATGGC TGAGAGTACA GAGAAGCACG CCAGACTTCA GGCAGTGATG 
TGTATCATCA GTACTATCAT GGAATCCTGC CCCTCCACCT CCAGCTTCTA CAGTAGTGCT 
ACAGCCAAGA CTCAGCATAA TGGCATGAAC AACATCATTC GACTGTTCCT AAAGAAGGGA 
CTAGTCAATG ACCTGGCCAG AGTTCCTCAC AGCCTAGACC TGTCCAGTCC CAATATGGCC 
AACACAGTCA ATGCTGCTCT AAAACCCTTG GAAACACTTT CCCGGATTGT AAATCAACCC 
AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT AGCAAGAACA AGTCTGAGCA GGATGCTCAA 
GGAGCTTCTC AGGACTCCAG CAGCCATCAG CAAGATCCAG GCGAGCCTGG GGAGGCAGAA 
GTGCAGGAGG AGGATCATGA TGTCACTCAG ACAGAGGTGG CAGATGGGGA TATCATGGAT 
GGGGAGGCTG AAACCGACTC AGTGGTGATT GCCGGGCAGC CTGAGGTGCT CAGTTCACAA 
GAGATGCAGG TTGAAAATGA ACTGGAGGAC CTGATAGATG AGTTGCTTGA GAGGGATGGC 
GGATCTGGGA ACAGTACAAT TATAGTGAGC AGAAGTGGAG AGGATGAATC ACAAGAGGAC 
GTGCTGATGG ATGAAGCTCC TTCCAACCTC AGCCAAGCTT CCACCTTGCA GGCCAACCGA 
GAAGATTCCA TGAATATCCT GGACCCTGAG GATGAGGAGG AACATACTCA GGAAGAGGAC 
AGCAGTGGCA GTAACGAGGA TGAGGATGAT AGTCAGGATG AAGAGGAGGA GGAGGAGGAA 
GATGAGGAAG ATGATCAGGA GGATGATGAA GGTGAAGAAG GAGATGAAGA TGATGACGAC 
GATGGTTCTG AGATGGAATT GGATGAGGAT TACCCTGATA TGAACGCTTC TCCCTTGGTC 
CGATTTGAGC GCTTTGACCG GGAGGATGAT CTCATCATTG AGTTTGACAA CATGTTCTCC 
AGTGCTACAG ACATTCCCCC ATCCCCAGGA AATATCCCTA CCACCCACCC ATTGATGGTA 
CGTCATGCAG ATCACAGTTC CCTGACACTG GGCAGTGGTT CTTCCACAAC TCGTCTTACC 
CAGGGCATCG GTCGCAGTCA AAGGACCCTC AGACAGCTCA CTGCCAATAC AGGCCATACC 
ATTCATGTTC ACTACCCTGG GAATCGCCAG CCTAACCCTC CTCTTATACT CCAGAGGTTG 
CTTGGTCCCT CTGCAGCTGC TGACATTCTT CAGCTGAGCA GCAGCCTTCC ATTACAAAGC 
CGAGGCCGGG CCCGCCTCCT AGTGGGCAAT GATGACGTCC ATATCATCGC CAGGTCTGAT 
GACGAGCTGC TGGATGACTT TTTTCATGAC CAGAGTACAG CCACTAGCCA AGCAGGAACC 
CTGTCCAGCA TCCCAACAGC TCTAACCCGA TGGACAGAGG AATGCAAAGT TCTTGATGCT 
GAGAGTATGC ATGACTGTGT TTCAGTGGTT AAAGTGCCCA TTGTCAATCA CCTGGAATTC 
TTGAGAGATG AGGAGCTAGA AGAGAGAAGG GAGAAACGCA GGAAACAATT GGCTGAGGAA 
GAAACAAAAA TAATAGACAA AGGCAAAGAA GACAAGGAGA ACAGAGATCA GAGTGCACAG 
TGTACTGTAA CTAAGACAAA TGACTCCACT GAACAGAATG TCTCAGATGG GACTCCTATG 
CCTGACAGCT ATCCAACAAC CCCCTCTTCA ACGGATGCAC CTACATCTGA GTCCAAGGAA 
ACACTTGGTA CCCTACAACC CTCCCAGCAA CAGCCAGCAC TTCCACCCCC ACCATCCTTA 
GGAGAGATTC CTCAGGAACT ACAGTCCCCT GCTGAAGAAG TGGCAAACTC TACACAGCTA 
TTGATGCCTA TAGAGTTGGA GGAATTGGGT CCCACAAGAC CAAGTGGAGA AGCAGAAACA 
ACTCAAATGG AATTGTCTCC AGCTCCCACC ATAACCTCTC TTTCCCCAGA GAGAGCTGAA 
GATTCAGACG CGTTAACAGC TGTCAGCAGT CAGCTGGAAG GTTCTCCCAT GGACACCAGC 
AGCCTGGCTT CCTGTACGTT AGAAGAGGCT GTGGGTGACA CTCCAGCAGC TGGCAGTTCT 
GAGCAGCCCA CAGCAGGTAG TTCCACTCCT GGGGATGCGC CATCAGTTGT GGCAGAAGTG 
CAAGGCAGAC CTGATGTATC AAGAGAATCC AACCAGCCAC CTGAGGACAG CTCCCCCCCT 
GCATCCTCGG AGAGTTCTTC CACTAGAGAT TCTGCCGTGG CCATCTCTGG AGCAGATTCT 
CGTGGAATTC TAGAAGAGCC ACTACCTTCA ACAAGCAGTG AAGAAGAAGA TCCCCTTGCT 
GGTATCAGTC TCCCTGAAGG TGTGGATCCC TCCTTTCTGG CTGCTCTCCC TGATGATATC 
CGTCGGGAAG TCCTACAGAA CCAGCTGGGC ATACGTCCAC CAACCCGAAG TGCTCCCTCC 
TCAAATAGCT CAGCTCCTGC AGTAGTGGGG AACCCTGGTG TAACTGAAGT GAGCCCTGAG 
TTCCTGGCTG CCCTACCTCC AGCTATTCAA GAGGAAGTGC TAGCACAGCA GAGAGCTGAA 
CAGCAGCGAC GGGAACTGGC TCAGAATGCG AGCTCAGACA CCCCCATGGA CCCTGTGACT 
TTCATTCAGA CTTTGCCCTC AGATCTGCGC CGTAGTGTCC TAGAGGATAT GGAAGACAGT 
GTGTTAGCTG TGATGCCACC TGACATTGCA GCTGAGGCTC AAGCTCTAAG ACGAGAACAA 
GAAGCCCGAC AGCGGCAGCT CATGCATGAG CGTCTTTTTG GACACAGTAG TACTTCTGCA 
CTCTCTGCCA TTCTCCGAAG CCCGGCTTTT ACCAGTCGCC TGAGTGGCAA CCGTGGGGTC 
CAGTATACTC GCCTTGCTGT ACAGAGGGGT GGCACCTTCC AGATGGGGGG TAGCAGCAGC 
CATAATAGGC CTTCTGGCAG TAATGTGGAC ACACTACTCC GCCTCCGAGG ACGACTCCTT 
CTAGACCATG AAGCCCTGTC TTGCCTCTTG GTCCTACTTT TTGTGGATGA GCCAAAGCTC 
AATACTAGCC GTCTACACCG AGTACTGAGA AATCTCTGCT ATCATGCCCA GACCCGCCAC 
TGGGTCATCC GCAGCCTCCT GTCCATTTTG CAGCGCAGCA GTGAAAGTGA GTTATGCATT 
GAGACACCTA AGCTCTCCAC AAGTGAAGAA AGGGGCAAGA AATCAAGCAA GAGCTGTGCA 
TCAAGCAGTC ATGAGAACCG TCCCCTGGAT CTGCTCCACA AGATGGAGTC CAAAAGTTCC 
AATCAGCTCT CCTGGCTCTC AGTATCAATG GATGCTGCTT TAGGCTGTAG GACTAACATA 
TTCCAGATTC AACGTTCTGG AGGGCGTAAA CATACAGAGA AGCATGCAAG CAGTGGTTCC 
ACTGTCCACA TCCACCCCCA GGCTGCTCCT GTTGTTTGCA GACATGTTTT GGATACACTC 
ATTCAGTTGG CCAAGGTGTT TCCCAGCCAC TTTACACAGC AGCGGACCAA AGAAACAAAC 
TGTGAGAGTG ATCGGGAACG GGGCAGTAAG CAGGCCTGCA GCCCATGCTC CTCACAGTCC 
TCCAGCAGTG GCATCTGTAC AGACTTCTGG GACTTATTGG TAAAACTGGA CAACATGAAT 
GTTAGCCGAA AAGGCAAGAA CTCCGTGAAG TCAGTACCAG TGAGCTCTGG TGGTGAAGGG 
GAAACTTCCC CACACAGCCT TGAAGCCTCT CCACTGGGGC AGCTCATGAA CATGTTGTCA 
CATCCAGTCA TCCGCCGGAG TTCTCTCTTA ACTGAGAAAC TCCTGAGACT CCTCTCTCTC 
ATCTCAATTG CTCTCCCAGA AAACAAAGTA TCAGAAGTGC AAACTAACTC CAGCAACAGT 
GGTTCCTCCA CTGCTGCCAC CTCAAACACA TCTACTACTA CCACCACCAC CACTACTGCC 
ACTGCCCCCA CGCCCACACC CCCAGCTGCA ACTACCCCTG TCACTTCTGC TCCAGCTCTG 
GTTGCTGCCA CAGCTATTTC CACCATTACT GTAGCTGCTT CTACCACAGT GACTACCCCC 
ACAACTGCTA CCACTACTGT TTCAACTTCT ACTACTAAAG GCAGCAAATC TCCAGCAAAG 
GTTGGTGAAG GAGGCAGTGG TATAGACTTC AAAATGGTGT CCTCTGGCCT CACTGAAAAC 
CAGCTACAGC TCTCTGTAGA GGTGTTGACA TCCCACTCTT GTTCTGAAGA AGGCTTGGAA 
GATGCGGCCA ATGTGTTACT GCAGCTCTCT CGTGGGGACT CTGGTACCCG GGACACTGTT 
CTTAAGCTGC TACTAAATGG AGCCCGCCAT CTGGGTTATA CCCTCTGTAA ACAGATAGGT 
ACCCTACTCG CTGAACTACG GGAATATAAT CTGGAGCAGC AGCGGCGAGC CCAATGCGAG 
ACCCTCTCTC CTGATGGCCT ACCTGAGGAG CAACCACAGA CCACCAAACT GAAAGGCAAA 
ATGCAGAGCA GGTTTGACAT GGCTGAAAAT GTGGTAATTG TGGCATCTCA GAAGCGACCC 
TTGGGTGGCC GGGAGCTCCA GCTGCCTTCT ATGTCCATGT TGACATCCAA GACATCTACC 
CAGAAGTTCT TCTTGAGGGT ACTACAGGTC ATCATCCAGC TGCGGGATGA CACACGACGA 
GCTAACAAGA AAGCCAAGCA GACAGGCAGG CTAGGTTCCT CCGGTTTAGG CTCAGCTAGC 
AGCATCCAGG CAGCTGTTCG GCAGCTGGAG GCTGAGGCTG ATGCCATTAT ACAAATGGTA 
CGTGAGGGTC AAAGGGCGCG GAGACAGCAA CAAGCAGCAA CGTCGGAGTC TTCTAACCAG 
TCAGAGACAT CTGTCCGGAG AGAGGAATCG CCCATGGATG TGGACCAGCC ATCTCCTAGT 
GCTCAAGATA CTCAGTCCAT TGTAATCTCA GATGGAACAC CACAGGGAGA GAAGGAGAAA 
GAGGAGAAAC CACCTGAGTT ACCCCTGCTC AGTGAGCAAC TGAGCCTAGA TGAACTGTGG 
GACATGCTAG GGGAATGTTT AAAAGAACTG GAGGAATCTC ATGACCAGCA TGCAGTATTA 
GTGCTACAGC CTGCTGTCGA GGCCTTCTTT TTGGTCCATG CCACAGAGCG GGAGAGCAAG 
CCTCCTGTCC GAGATACCCG TGAGAGCCAG CTGGCACACA TCAAGGATGA ACCTCCTCCA 
CTCTCCCCCG CCCCCTTAAC CCCAGCCACT CCATCCTCCC TTGACCCATT CTTCTCCAGG 
GAGCCCTCAT CTATGCACAT TTCCTCGAGC CTGCCCCCTG ACACACAGAA GTTCCTTCGC 
TTTGCAGAGA CTCACCGCAC TGTGTTAAAC CAGATACTAC GGCAGTCTAC CACCCATCTT 
GCTGATGGGC CTTTTGCTGT CTTGGTAGAT TATATCCGTG TCCTCGACTT TGATGTCAAG 
CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTGGACGAAG GGCTCCGGAA AGAAGATATG 
GCAGTGCATG TCCGCCGTGA CCATGTATTT GAAGACTCCT ATCGTGAGCT GCATCGCAAA 
TCTCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTGTTTG AAGGAGAAGA AGGGCAGGAT 
GCTGGGGGGC TCCTGCGAGA GTGGTACATG ATCATCTCTC GAGAGATGTT CAACCCTATG 
TATGCCTTGT TCCGTACCTC ACCTGGTGAC CGGGTCACCT ACACCATCAA TCCATCTTCC 
CACTGTAACC CCAACCACCT CAGCTACTTC AAGTTTGTTG GACGAATTGT AGCCAAAGCT 
GTATATGACA ACCGCCTCCT GGAGTGCTAC TTTACTAGGT CTTTCTACAA ACACATCTTG 
GGCAAATCTG TCAGGTATAC AGATATGGAG AGTGAAGATT ACCACTTCTA CCAGGGCCTA 
GTTTATCTGC TTGAAAATGA TGTCTCTACA TTAGGCTATG ACCTCACCTT CAGCACTGAG 
GTTCAAGAAT TTGGAGTATG TGAAGTTCGT GACCTCAAAC CCAATGGAGC CAACATCTTG 
GTAACAGAGG AGAACAAGAA GGAATATGTA CACCTGGTTT GCCAGATGAG AATGACAGGA 
GCCATCCGCA AACAGCTGGC AGCCTTCTTG GAGGGCTTCT ATGAAATCAT TCCAAAGCGC 
CTCATATCCA TCTTTACTGA GCAGGAATTA GAGCTGCTCA TATCAGGGCT GCCTACCATC 
GACATTGATG ATCTAAAATC TAACACTGAG TACCACAAGT ACCAATCCAA CTCTATTCAG 
ATCCAGTGGT TTTGGAGAGC ATTGCGTTCC TTTGACCAAG CAGACCGTGC CAAGTTCCTC 
CAGTTTGTCA CAGGTACTTC CAAGGTACCC CTACAAGGAT TTGCTGCCCT TGAAGGTATG 
AATGGCATTC AGAAGTTTCA GATTCATCGA GATGACAGAT CCACAGATCG CCTGCCTTCA 
GCTCACACAT GTTTTAATCA ACTGGACCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC 
CACATGCTAC TATTGGCCAT CCAGGAATGC TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246161.1
CDS	1191..14324
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEDKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_011247859.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247859.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGAGAAC AGGATGGAAC TGCTGGAAGC 
ATGGATGCAT CTGCCCAGGG TTTATTAGAA GGAATTGAAC TAGATGGTGA CACACTGGCA 
CCCATGGAGA CAGATGAACC TTCTTCTTCA GATTCTAAGG GCAAATCTAA AATCACACCT 
GCCATGGCTG CAAGAATTAA ACAAATCAAG CCGTTGCTGT CAGCTTCTTC CAGATTAGGC 
CGAGCACTTG CTGAGCTCTT TGGACTCCTT GTTAAGCTTT GTGTGGGTTC TCCTGTCCGC 
CAAAGGAGGA GCCATCATGC TGCCAGTACC ACCACTGCAC CAACACCTGC TGCTAGATCA 
ACAGCTTCAG CCCTCACTAA GCTACTCACA AAAGGCTTAT CATGGCAGCC CCCACCATAT 
ACACCCACTC CCCGCTTCAG GCTGACGTTC TTCATCTGTT CAGTTGGATT CACCTCTCCA 
ATGCTCTTTG ATGAGAGAAA GTATCCTTAC CACCTTATGC TGCAGAAATT TCTCTGCTCT 
GGTGGTCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TATCCATGGG AGGAAAAGTT 
CCTGTTTCTG AGGGATTGGA ACATTCAGAT CTACCTGATG GTACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTTGTAGA AAAGATGGTA AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGTTGCCTG CTAAATTGCC TGGAGGAGTC CAGAGCTTTC CACAATTTAG TGCCCTTCGC 
TTCCTTGTAG TAACTCAAAA AGCAGCCTTT ACTTGTATCA AGAACCTGTG GAACCGGAAA 
CCCCTTAAAG TATATGGGGG AAGAATGGCT GAGTCTATGC TGGCCATTTT GTGCCACATT 
CTCCGAGGGG AACCTGTGAT TCGAGAAAGA CTGAGCAAAG AGAAGGAAGG ATCTCGAGGA 
GAAGAAGAAG CAGGCCAGGA AGAAGGTGGC TCACGCAGAG AACCTCAAGT CAACCAGCAA 
CAGCTACAAC AGCTCATGGA CATGGGTTTC ACAAGAGAAC ATGCCATGGA GGCACTGTTG 
AATACCAGTA CGATGGAACA AGCAACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATA 
GGAGGAGTTG TTCGGGATCT TAGCATGTCA GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAAGA GCGTAAAGCT CGAGAAAAAC AAGAAGAGGA AGAAGCTAAA 
TGTCTAGAGA AGTTTCAAGA TGCTGACCCC TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACAATGT TACCAGGCTG CTTCCATCTT CTTGATGAGT TGCCTGATAC AGTGTACCGT 
GTTTGTGATC TGATCATGAC AGCTATCAAA CGTAATGGAG CTGACTATCG TGATATGATT 
CTAAAGCAAG TTGTCAACCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCTCTAACAA CAAGTGACAC AAAAACAGTT TCAGAGTGGA TCAGTCAGAT GGCTACCCTG 
CCACAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CACTACTTTT TGAGGAGTTG 
AAGTTACCTT GTGCCTGGGT GGTTGAATCA AGTGGCATTC TTAATGTCCT AATCAAACTC 
TTAGAAGTGG TTCAACCCTG CCTACAGGCT GCCAAAGAGC AAAAGGAAGT CCAGACCCCA 
AAGTGGATCA CCCCAGTGTT GCTCCTTATT GATTTTTATG AAAAGACAGC CATCTCATCA 
AAGAGAAGAG CCCAAATGAC TAAGTACCTA CAGTCCAACA GCAACAACTG GCGCTGGTTT 
GATGACCGCT CTGGGCGTTG GTGTAGTTAC AGTGCTAGCA ACAACAGCAC CATTGATTCT 
GCCTGGAAAT CTGGAGAAAC AAGTGTAAGA TTCACTGCTG GCCGACGAAG ATACACTGTC 
CAGTTCACTA CAATGGTGCA GGTTAATGAA GAAACAGGAA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTGCCACG ACTGAGTAAG AACTCAAAAA GCAGCAATGG ACAGGAACTA 
GAGAAGACAC TGGAAGAAAG CAAGGAGACC GATATCAAAC ATAAAGAAAA TAAAGGCAAT 
GATATCCCCT TAGCTCTAGA GAGCACAAAC ACTGAAAAAG AGGCAAGCCT GGATGAAACA 
AAAATTGGGG AGATCCTAAT CCAGGGCCTG ACAGAAGATA TGGTGACTGT TCTAATCCGT 
GCCTGTGTGA GCATGCTGGG AGTCCCTGTG GACCCAGACA CTTTGCATGC CACCCTTCGT 
CTCTGTTTAA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACACGAATGA TCTTGAATTT GACCCAGAGC TCAGGTTTCA ATGGGTTTAC CCCCTTAGTC 
ACCCTTCTCT TAAGGCACAT CATTGAGGAT CCTTGTACTC TTCGTCATAC TATGGAAAAG 
GTTGTTCGCT CGGCAGCCAC AAGTGGAGCT GGTAGTACTA CATCTGGTGT TGTATCTGGC 
AGCCTCGGTT CTCGGGAGAT CAACTATATC CTTAGAGTCC TAGGACCAGC TGCCTGCCGC 
AATCCAGACA TATTCACAGA AGTAGCCAAT TGCTGTATCC GCATTGCCCT TCCAGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGTCCTAAT 
GCTGTACAGC TGGTAAAGAC AACACCTTTG AAGCCCTCAT CTCTGCCTGT CATTCCTGAT 
ACTATTAAGG AAGTTATCTA TGATATGCTG AATGCTTTGG CTGCATACCA TGCTCCAGAG 
GAAGACAAAT CTGATCCTAA ACCTGGGGGA ACGACCCAAG AGGTTGGCCA GCTCCTGCAA 
GACATGGGTG ACGATGTATA CCAGCAGTAC CGGTCACTTA CTCGTCAGAG CAGTGACTTT 
GATACACAGT CAGGTTTTTC CTTAAATAGT CAGGTTTTTG CTGCAGATGG TGCCCCTGCT 
GAAACTTCCA CAACTGGAAC CTCCCAAGGA GAGGGAGCTT CAACTCCAGA GGAGACTCGA 
GAAGGGAAGA AAGATAAAGA AGGGGACCGT ACATCTGAGG AAGGCAAGCA GAAAAGCAAG 
GGCAGCAAAC CTTTAATGCC TACCTCTACT ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG 
TCCTATGTTG GTATTGCTAC CCTGATTGCA AACTACAGCT ATACCGTGGG CCAGTCTGAG 
CTGATCAAAG AGGACTGCAG TGTGCTAGCT TTTGTCCTGG ACCACCTCCT CCCACACACC 
CAGAATGCAG AAGACAAGGA TACACCTGCC CTGGCACGTC TATTCCTTGC AAGTCTGGCT 
GCTGCGGGGA GTGGCACAGA TGCTCAAGTG GCCCTAGTGA ATGAAGTGAA AGCAGCCCTG 
GGACGGGCGC TTGCTATGGC TGAGAGTACA GAGAAGCACG CCAGACTTCA GGCAGTGATG 
TGTATCATCA GTACTATCAT GGAATCCTGC CCCTCCACCT CCAGCTTCTA CAGTAGTGCT 
ACAGCCAAGA CTCAGCATAA TGGCATGAAC AACATCATTC GACTGTTCCT AAAGAAGGGA 
CTAGTCAATG ACCTGGCCAG AGTTCCTCAC AGCCTAGACC TGTCCAGTCC CAATATGGCC 
AACACAGTCA ATGCTGCTCT AAAACCCTTG GAAACACTTT CCCGGATTGT AAATCAACCC 
AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT AGCAAGAACA AGTCTGAGCA GGATGCTCAA 
GGAGCTTCTC AGGACTCCAG CAGCCATCAG CAAGATCCAG GCGAGCCTGG GGAGGCAGAA 
GTGCAGGAGG AGGATCATGA TGTCACTCAG ACAGAGGTGG CAGATGGGGA TATCATGGAT 
GGGGAGGCTG AAACCGACTC AGTGGTGATT GCCGGGCAGC CTGAGGTGCT CAGTTCACAA 
GAGATGCAGG TTGAAAATGA ACTGGAGGAC CTGATAGATG AGTTGCTTGA GAGGGATGGC 
GGATCTGGGA ACAGTACAAT TATAGTGAGC AGAAGTGGAG AGGATGAATC ACAAGAGGAC 
GTGCTGATGG ATGAAGCTCC TTCCAACCTC AGCCAAGCTT CCACCTTGCA GGCCAACCGA 
GAAGATTCCA TGAATATCCT GGACCCTGAG GATGAGGAGG AACATACTCA GGAAGAGGAC 
AGCAGTGGCA GTAACGAGGA TGAGGATGAT AGTCAGGATG AAGAGGAGGA GGAGGAGGAA 
GATGAGGAAG ATGATCAGGA GGATGATGAA GGTGAAGAAG GAGATGAAGA TGATGACGAC 
GATGGTTCTG AGATGGAATT GGATGAGGAT TACCCTGATA TGAACGCTTC TCCCTTGGTC 
CGATTTGAGC GCTTTGACCG GGAGGATGAT CTCATCATTG AGTTTGACAA CATGTTCTCC 
AGTGCTACAG ACATTCCCCC ATCCCCAGGA AATATCCCTA CCACCCACCC ATTGATGGTA 
CGTCATGCAG ATCACAGTTC CCTGACACTG GGCAGTGGTT CTTCCACAAC TCGTCTTACC 
CAGGGCATCG GTCGCAGTCA AAGGACCCTC AGACAGCTCA CTGCCAATAC AGGCCATACC 
ATTCATGTTC ACTACCCTGG GAATCGCCAG CCTAACCCTC CTCTTATACT CCAGAGGTTG 
CTTGGTCCCT CTGCAGCTGC TGACATTCTT CAGCTGAGCA GCAGCCTTCC ATTACAAAGC 
CGAGGCCGGG CCCGCCTCCT AGTGGGCAAT GATGACGTCC ATATCATCGC CAGGTCTGAT 
GACGAGCTGC TGGATGACTT TTTTCATGAC CAGAGTACAG CCACTAGCCA AGCAGGAACC 
CTGTCCAGCA TCCCAACAGC TCTAACCCGA TGGACAGAGG AATGCAAAGT TCTTGATGCT 
GAGAGTATGC ATGACTGTGT TTCAGTGGTT AAAGTGCCCA TTGTCAATCA CCTGGAATTC 
TTGAGAGATG AGGAGCTAGA AGAGAGAAGG GAGAAACGCA GGAAACAATT GGCTGAGGAA 
GAAACAAAAA TAATAGACAA AGGCAAAGAA GACAAGGAGA ACAGAGATCA GAGTGCACAG 
TGTACTGTAA CTAAGACAAA TGACTCCACT GAACAGAATG TCTCAGATGG GACTCCTATG 
CCTGACAGCT ATCCAACAAC CCCCTCTTCA ACGGATGCAC CTACATCTGA GTCCAAGGAA 
ACACTTGGTA CCCTACAACC CTCCCAGCAA CAGCCAGCAC TTCCACCCCC ACCATCCTTA 
GGAGAGATTC CTCAGGAACT ACAGTCCCCT GCTGAAGAAG TGGCAAACTC TACACAGCTA 
TTGATGCCTA TAGAGTTGGA GGAATTGGGT CCCACAAGAC CAAGTGGAGA AGCAGAAACA 
ACTCAAATGG AATTGTCTCC AGCTCCCACC ATAACCTCTC TTTCCCCAGA GAGAGCTGAA 
GATTCAGACG CGTTAACAGC TGTCAGCAGT CAGCTGGAAG GTTCTCCCAT GGACACCAGC 
AGCCTGGCTT CCTGTACGTT AGAAGAGGCT GTGGGTGACA CTCCAGCAGC TGGCAGTTCT 
GAGCAGCCCA CAGCAGGTAG TTCCACTCCT GGGGATGCGC CATCAGTTGT GGCAGAAGTG 
CAAGGCAGAC CTGATGTATC AAGAGAATCC AACCAGCCAC CTGAGGACAG CTCCCCCCCT 
GCATCCTCGG AGAGTTCTTC CACTAGAGAT TCTGCCGTGG CCATCTCTGG AGCAGATTCT 
CGTGGAATTC TAGAAGAGCC ACTACCTTCA ACAAGCAGTG AAGAAGAAGA TCCCCTTGCT 
GGTATCAGTC TCCCTGAAGG TGTGGATCCC TCCTTTCTGG CTGCTCTCCC TGATGATATC 
CGTCGGGAAG TCCTACAGAA CCAGCTGGGC ATACGTCCAC CAACCCGAAG TGCTCCCTCC 
TCAAATAGCT CAGCTCCTGC AGTAGTGGGG AACCCTGGTG TAACTGAAGT GAGCCCTGAG 
TTCCTGGCTG CCCTACCTCC AGCTATTCAA GAGGAAGTGC TAGCACAGCA GAGAGCTGAA 
CAGCAGCGAC GGGAACTGGC TCAGAATGCG AGCTCAGACA CCCCCATGGA CCCTGTGACT 
TTCATTCAGA CTTTGCCCTC AGATCTGCGC CGTAGTGTCC TAGAGGATAT GGAAGACAGT 
GTGTTAGCTG TGATGCCACC TGACATTGCA GCTGAGGCTC AAGCTCTAAG ACGAGAACAA 
GAAGCCCGAC AGCGGCAGCT CATGCATGAG CGTCTTTTTG GACACAGTAG TACTTCTGCA 
CTCTCTGCCA TTCTCCGAAG CCCGGCTTTT ACCAGTCGCC TGAGTGGCAA CCGTGGGGTC 
CAGTATACTC GCCTTGCTGT ACAGAGGGGT GGCACCTTCC AGATGGGGGG TAGCAGCAGC 
CATAATAGGC CTTCTGGCAG TAATGTGGAC ACACTACTCC GCCTCCGAGG ACGACTCCTT 
CTAGACCATG AAGCCCTGTC TTGCCTCTTG GTCCTACTTT TTGTGGATGA GCCAAAGCTC 
AATACTAGCC GTCTACACCG AGTACTGAGA AATCTCTGCT ATCATGCCCA GACCCGCCAC 
TGGGTCATCC GCAGCCTCCT GTCCATTTTG CAGCGCAGCA GTGAAAGTGA GTTATGCATT 
GAGACACCTA AGCTCTCCAC AAGTGAAGAA AGGGGCAAGA AATCAAGCAA GAGCTGTGCA 
TCAAGCAGTC ATGAGAACCG TCCCCTGGAT CTGCTCCACA AGATGGAGTC CAAAAGTTCC 
AATCAGCTCT CCTGGCTCTC AGTATCAATG GATGCTGCTT TAGGCTGTAG GACTAACATA 
TTCCAGATTC AACGTTCTGG AGGGCGTAAA CATACAGAGA AGCATGCAAG CAGTGGTTCC 
ACTGTCCACA TCCACCCCCA GGCTGCTCCT GTTGTTTGCA GACATGTTTT GGATACACTC 
ATTCAGTTGG CCAAGGTGTT TCCCAGCCAC TTTACACAGC AGCGGACCAA AGAAACAAAC 
TGTGAGAGTG ATCGGGAACG GGGCAGTAAG CAGGCCTGCA GCCCATGCTC CTCACAGTCC 
TCCAGCAGTG GCATCTGTAC AGACTTCTGG GACTTATTGG TAAAACTGGA CAACATGAAT 
GTTAGCCGAA AAGGCAAGAA CTCCGTGAAG TCAGTACCAG TGAGCTCTGG TGGTGAAGGG 
GAAACTTCCC CACACAGCCT TGAAGCCTCT CCACTGGGGC AGCTCATGAA CATGTTGTCA 
CATCCAGTCA TCCGCCGGAG TTCTCTCTTA ACTGAGAAAC TCCTGAGACT CCTCTCTCTC 
ATCTCAATTG CTCTCCCAGA AAACAAAGTA TCAGAAGTGC AAACTAACTC CAGCAACAGT 
GGTTCCTCCA CTGCTGCCAC CTCAAACACA TCTACTACTA CCACCACCAC CACTACTGCC 
ACTGCCCCCA CGCCCACACC CCCAGCTGCA ACTACCCCTG TCACTTCTGC TCCAGCTCTG 
GTTGCTGCCA CAGCTATTTC CACCATTACT GTAGCTGCTT CTACCACAGT GACTACCCCC 
ACAACTGCTA CCACTACTGT TTCAACTTCT ACTACTAAAG GCAGCAAATC TCCAGCAAAG 
GTTGGTGAAG GAGGCAGTGG TATAGACTTC AAAATGGTGT CCTCTGGCCT CACTGAAAAC 
CAGCTACAGC TCTCTGTAGA GGTGTTGACA TCCCACTCTT GTTCTGAAGA AGGCTTGGAA 
GATGCGGCCA ATGTGTTACT GCAGCTCTCT CGTGGGGACT CTGGTACCCG GGACACTGTT 
CTTAAGCTGC TACTAAATGG AGCCCGCCAT CTGGGTTATA CCCTCTGTAA ACAGATAGGT 
ACCCTACTCG CTGAACTACG GGAATATAAT CTGGAGCAGC AGCGGCGAGC CCAATGCGAG 
ACCCTCTCTC CTGATGGCCT ACCTGAGGAG CAACCACAGA CCACCAAACT GAAAGGCAAA 
ATGCAGAGCA GGTTTGACAT GGCTGAAAAT GTGGTAATTG TGGCATCTCA GAAGCGACCC 
TTGGGTGGCC GGGAGCTCCA GCTGCCTTCT ATGTCCATGT TGACATCCAA GACATCTACC 
CAGAAGTTCT TCTTGAGGGT ACTACAGGTC ATCATCCAGC TGCGGGATGA CACACGACGA 
GCTAACAAGA AAGCCAAGCA GACAGGCAGG CTAGGTTCCT CCGGTTTAGG CTCAGCTAGC 
AGCATCCAGG CAGCTGTTCG GCAGCTGGAG GCTGAGGCTG ATGCCATTAT ACAAATGGTA 
CGTGAGGGTC AAAGGGCGCG GAGACAGCAA CAAGCAGCAA CGTCGGAGTC TTCTAACCAG 
TCAGAGACAT CTGTCCGGAG AGAGGAATCG CCCATGGATG TGGACCAGCC ATCTCCTAGT 
GCTCAAGATA CTCAGTCCAT TGTAATCTCA GATGGAACAC CACAGGGAGA GAAGGAGAAA 
GAGGAGAAAC CACCTGAGTT ACCCCTGCTC AGTGAGCAAC TGAGCCTAGA TGAACTGTGG 
GACATGCTAG GGGAATGTTT AAAAGAACTG GAGGAATCTC ATGACCAGCA TGCAGTATTA 
GTGCTACAGC CTGCTGTCGA GGCCTTCTTT TTGGTCCATG CCACAGAGCG GGAGAGCAAG 
CCTCCTGTCC GAGATACCCG TGAGAGCCAG CTGGCACACA TCAAGGATGA ACCTCCTCCA 
CTCTCCCCCG CCCCCTTAAC CCCAGCCACT CCATCCTCCC TTGACCCATT CTTCTCCAGG 
GAGCCCTCAT CTATGCACAT TTCCTCGAGC CTGCCCCCTG ACACACAGAA GTTCCTTCGC 
TTTGCAGAGA CTCACCGCAC TGTGTTAAAC CAGATACTAC GGCAGTCTAC CACCCATCTT 
GCTGATGGGC CTTTTGCTGT CTTGGTAGAT TATATCCGTG TCCTCGACTT TGATGTCAAG 
CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTGGACGAAG GGCTCCGGAA AGAAGATATG 
GCAGTGCATG TCCGCCGTGA CCATGTATTT GAAGACTCCT ATCGTGAGCT GCATCGCAAA 
TCTCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTGTTTG AAGGAGAAGA AGGGCAGGAT 
GCTGGGGGGC TCCTGCGAGA GTGGTACATG ATCATCTCTC GAGAGATGTT CAACCCTATG 
TATGCCTTGT TCCGTACCTC ACCTGGTGAC CGGGTCACCT ACACCATCAA TCCATCTTCC 
CACTGTAACC CCAACCACCT CAGCTACTTC AAGTTTGTTG GACGAATTGT AGCCAAAGCT 
GTATATGACA ACCGCCTCCT GGAGTGCTAC TTTACTAGGT CTTTCTACAA ACACATCTTG 
GGCAAATCTG TCAGGTATAC AGATATGGAG AGTGAAGATT ACCACTTCTA CCAGGGCCTA 
GTTTATCTGC TTGAAAATGA TGTCTCTACA TTAGGCTATG ACCTCACCTT CAGCACTGAG 
GTTCAAGAAT TTGGAGTATG TGAAGTTCGT GACCTCAAAC CCAATGGAGC CAACATCTTG 
GTAACAGAGG AGAACAAGAA GGAATATGTA CACCTGGTTT GCCAGATGAG AATGACAGGA 
GCCATCCGCA AACAGCTGGC AGCCTTCTTG GAGGGCTTCT ATGAAATCAT TCCAAAGCGC 
CTCATATCCA TCTTTACTGA GCAGGAATTA GAGCTGCTCA TATCAGGGCT GCCTACCATC 
GACATTGATG ATCTAAAATC TAACACTGAG TACCACAAGT ACCAATCCAA CTCTATTCAG 
ATCCAGTGGT TTTGGAGAGC ATTGCGTTCC TTTGACCAAG CAGACCGTGC CAAGTTCCTC 
CAGTTTGTCA CAGGTACTTC CAAGGTACCC CTACAAGGAT TTGCTGCCCT TGAAGGTATG 
AATGGCATTC AGAAGTTTCA GATTCATCGA GATGACAGAT CCACAGATCG CCTGCCTTCA 
GCTCACACAT GTTTTAATCA ACTGGACCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC 
CACATGCTAC TATTGGCCAT CCAGGAATGC TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_011247860.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246162.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X21, mRNA.

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CloneID	OMu69815
Clone ID Related Accession (Same CDS sequence)	XM_011247860.1 , XM_030251458.1
Accession Version	XM_011247860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGCAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246162.1
CDS	1191..14159
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIADIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_011247860.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247860.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGCAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251458.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107318.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X36, mRNA.

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CloneID	OMu69815
Clone ID Related Accession (Same CDS sequence)	XM_011247860.1 , XM_030251458.1
Accession Version	XM_030251458.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGCAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107318.1
CDS	1856..14824
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIADIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251458.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251458.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG CTTCAGATGA TGAATTTGAG AATCTTAGAA TTAAAGGTCC TAATGCTGTA 
CAGCTGGTAA AGACAACACC TTTGAAGCCC TCATCTCTGC CTGTCATTCC TGATACTATT 
AAGGAAGTTA TCTATGATAT GCTGAATGCT TTGGCTGCAT ACCATGCTCC AGAGGAAGCA 
GACAAATCTG ATCCTAAACC TGGGGGAACG ACCCAAGAGG TTGGCCAGCT CCTGCAAGAC 
ATGGGTGACG ATGTATACCA GCAGTACCGG TCACTTACTC GTCAGAGCAG TGACTTTGAT 
ACACAGTCAG GTTTTTCCTT AAATAGTCAG GTTTTTGCTG CAGATGGTGC CCCTGCTGAA 
ACTTCCACAA CTGGAACCTC CCAAGGAGAG GGAGCTTCAA CTCCAGAGGA GACTCGAGAA 
GGGAAGAAAG ATAAAGAAGG GGACCGTACA TCTGAGGAAG GCAAGCAGAA AAGCAAGGGC 
AGCAAACCTT TAATGCCTAC CTCTACTATC CTTCGTCTTC TGGCAGAGTT GGTGAGGTCC 
TATGTTGGTA TTGCTACCCT GATTGCAAAC TACAGCTATA CCGTGGGCCA GTCTGAGCTG 
ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT GTCCTGGACC ACCTCCTCCC ACACACCCAG 
AATGCAGAAG ACAAGGATAC ACCTGCCCTG GCACGTCTAT TCCTTGCAAG TCTGGCTGCT 
GCGGGGAGTG GCACAGATGC TCAAGTGGCC CTAGTGAATG AAGTGAAAGC AGCCCTGGGA 
CGGGCGCTTG CTATGGCTGA GAGTACAGAG AAGCACGCCA GACTTCAGGC AGTGATGTGT 
ATCATCAGTA CTATCATGGA ATCCTGCCCC TCCACCTCCA GCTTCTACAG TAGTGCTACA 
GCCAAGACTC AGCATAATGG CATGAACAAC ATCATTCGAC TGTTCCTAAA GAAGGGACTA 
GTCAATGACC TGGCCAGAGT TCCTCACAGC CTAGACCTGT CCAGTCCCAA TATGGCCAAC 
ACAGTCAATG CTGCTCTAAA ACCCTTGGAA ACACTTTCCC GGATTGTAAA TCAACCCAGT 
AGCCTTTTTG GCAGCAAGAG TGCTTCTAGC AAGAACAAGT CTGAGCAGGA TGCTCAAGGA 
GCTTCTCAGG ACTCCAGCAG CCATCAGCAA GATCCAGGCG AGCCTGGGGA GGCAGAAGTG 
CAGGAGGAGG ATCATGATGT CACTCAGACA GAGGTGGCAG ATGGGGATAT CATGGATGGG 
GAGGCTGAAA CCGACTCAGT GGTGATTGCC GGGCAGCCTG AGGTGCTCAG TTCACAAGAG 
ATGCAGGTTG AAAATGAACT GGAGGACCTG ATAGATGAGT TGCTTGAGAG GGATGGCGGA 
TCTGGGAACA GTACAATTAT AGCAGACATT CCCCCATCCC CAGGAAATAT CCCTACCACC 
CACCCATTGA TGGTACGTCA TGCAGATCAC AGTTCCCTGA CACTGGGCAG TGGTTCTTCC 
ACAACTCGTC TTACCCAGGG CATCGGTCGC AGTCAAAGGA CCCTCAGACA GCTCACTGCC 
AATACAGGCC ATACCATTCA TGTTCACTAC CCTGGGAATC GCCAGCCTAA CCCTCCTCTT 
ATACTCCAGA GGTTGCTTGG TCCCTCTGCA GCTGCTGACA TTCTTCAGCT GAGCAGCAGC 
CTTCCATTAC AAAGCCGAGG CCGGGCCCGC CTCCTAGTGG GCAATGATGA CGTCCATATC 
ATCGCCAGGT CTGATGACGA GCTGCTGGAT GACTTTTTTC ATGACCAGAG TACAGCCACT 
AGCCAAGCAG GAACCCTGTC CAGCATCCCA ACAGCTCTAA CCCGATGGAC AGAGGAATGC 
AAAGTTCTTG ATGCTGAGAG TATGCATGAC TGTGTTTCAG TGGTTAAAGT GCCCATTGTC 
AATCACCTGG AATTCTTGAG AGATGAGGAG CTAGAAGAGA GAAGGGAGAA ACGCAGGAAA 
CAATTGGCTG AGGAAGAAAC AAAAATAATA GACAAAGGCA AAGAAGACAA GGAGAACAGA 
GATCAGAGTG CACAGTGTAC TGTAACTAAG ACAAATGACT CCACTGAACA GAATGTCTCA 
GATGGGACTC CTATGCCTGA CAGCTATCCA ACAACCCCCT CTTCAACGGA TGCACCTACA 
TCTGAGTCCA AGGAAACACT TGGTACCCTA CAACCCTCCC AGCAACAGCC AGCACTTCCA 
CCCCCACCAT CCTTAGGAGA GATTCCTCAG GAACTACAGT CCCCTGCTGA AGAAGTGGCA 
AACTCTACAC AGCTATTGAT GCCTATAGAG TTGGAGGAAT TGGGTCCCAC AAGACCAAGT 
GGAGAAGCAG AAACAACTCA AATGGAATTG TCTCCAGCTC CCACCATAAC CTCTCTTTCC 
CCAGAGAGAG CTGAAGATTC AGACGCGTTA ACAGCTGTCA GCAGTCAGCT GGAAGGTTCT 
CCCATGGACA CCAGCAGCCT GGCTTCCTGT ACGTTAGAAG AGGCTGTGGG TGACACTCCA 
GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGTAGTTCCA CTCCTGGGGA TGCGCCATCA 
GTTGTGGCAG AAGTGCAAGG CAGACCTGAT GTATCAAGAG AATCCAACCA GCCACCTGAG 
GACAGCTCCC CCCCTGCATC CTCGGAGAGT TCTTCCACTA GAGATTCTGC CGTGGCCATC 
TCTGGAGCAG ATTCTCGTGG AATTCTAGAA GAGCCACTAC CTTCAACAAG CAGTGAAGAA 
GAAGATCCCC TTGCTGGTAT CAGTCTCCCT GAAGGTGTGG ATCCCTCCTT TCTGGCTGCT 
CTCCCTGATG ATATCCGTCG GGAAGTCCTA CAGAACCAGC TGGGCATACG TCCACCAACC 
CGAAGTGCTC CCTCCTCAAA TAGCTCAGCT CCTGCAGTAG TGGGGAACCC TGGTGTAACT 
GAAGTGAGCC CTGAGTTCCT GGCTGCCCTA CCTCCAGCTA TTCAAGAGGA AGTGCTAGCA 
CAGCAGAGAG CTGAACAGCA GCGACGGGAA CTGGCTCAGA ATGCGAGCTC AGACACCCCC 
ATGGACCCTG TGACTTTCAT TCAGACTTTG CCCTCAGATC TGCGCCGTAG TGTCCTAGAG 
GATATGGAAG ACAGTGTGTT AGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCT 
CTAAGACGAG AACAAGAAGC CCGACAGCGG CAGCTCATGC ATGAGCGTCT TTTTGGACAC 
AGTAGTACTT CTGCACTCTC TGCCATTCTC CGAAGCCCGG CTTTTACCAG TCGCCTGAGT 
GGCAACCGTG GGGTCCAGTA TACTCGCCTT GCTGTACAGA GGGGTGGCAC CTTCCAGATG 
GGGGGTAGCA GCAGCCATAA TAGGCCTTCT GGCAGTAATG TGGACACACT ACTCCGCCTC 
CGAGGACGAC TCCTTCTAGA CCATGAAGCC CTGTCTTGCC TCTTGGTCCT ACTTTTTGTG 
GATGAGCCAA AGCTCAATAC TAGCCGTCTA CACCGAGTAC TGAGAAATCT CTGCTATCAT 
GCCCAGACCC GCCACTGGGT CATCCGCAGC CTCCTGTCCA TTTTGCAGCG CAGCAGTGAA 
AGTGAGTTAT GCATTGAGAC ACCTAAGCTC TCCACAAGTG AAGAAAGGGG CAAGAAATCA 
AGCAAGAGCT GTGCATCAAG CAGTCATGAG AACCGTCCCC TGGATCTGCT CCACAAGATG 
GAGTCCAAAA GTTCCAATCA GCTCTCCTGG CTCTCAGTAT CAATGGATGC TGCTTTAGGC 
TGTAGGACTA ACATATTCCA GATTCAACGT TCTGGAGGGC GTAAACATAC AGAGAAGCAT 
GCAAGCAGTG GTTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT TTGCAGACAT 
GTTTTGGATA CACTCATTCA GTTGGCCAAG GTGTTTCCCA GCCACTTTAC ACAGCAGCGG 
ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAACGGGGCA GTAAGCAGGC CTGCAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATC TGTACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTTAG CCGAAAAGGC AAGAACTCCG TGAAGTCAGT ACCAGTGAGC 
TCTGGTGGTG AAGGGGAAAC TTCCCCACAC AGCCTTGAAG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACATCC AGTCATCCGC CGGAGTTCTC TCTTAACTGA GAAACTCCTG 
AGACTCCTCT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTATCAGA AGTGCAAACT 
AACTCCAGCA ACAGTGGTTC CTCCACTGCT GCCACCTCAA ACACATCTAC TACTACCACC 
ACCACCACTA CTGCCACTGC CCCCACGCCC ACACCCCCAG CTGCAACTAC CCCTGTCACT 
TCTGCTCCAG CTCTGGTTGC TGCCACAGCT ATTTCCACCA TTACTGTAGC TGCTTCTACC 
ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTTCTACTAC TAAAGGCAGC 
AAATCTCCAG CAAAGGTTGG TGAAGGAGGC AGTGGTATAG ACTTCAAAAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAAGAAGGCT TGGAAGATGC GGCCAATGTG TTACTGCAGC TCTCTCGTGG GGACTCTGGT 
ACCCGGGACA CTGTTCTTAA GCTGCTACTA AATGGAGCCC GCCATCTGGG TTATACCCTC 
TGTAAACAGA TAGGTACCCT ACTCGCTGAA CTACGGGAAT ATAATCTGGA GCAGCAGCGG 
CGAGCCCAAT GCGAGACCCT CTCTCCTGAT GGCCTACCTG AGGAGCAACC ACAGACCACC 
AAACTGAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AAAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCCTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTGCGG 
GATGACACAC GACGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTTCTA ACCAGTCAGA GACATCTGTC CGGAGAGAGG AATCGCCCAT GGATGTGGAC 
CAGCCATCTC CTAGTGCTCA AGATACTCAG TCCATTGTAA TCTCAGATGG AACACCACAG 
GGAGAGAAGG AGAAAGAGGA GAAACCACCT GAGTTACCCC TGCTCAGTGA GCAACTGAGC 
CTAGATGAAC TGTGGGACAT GCTAGGGGAA TGTTTAAAAG AACTGGAGGA ATCTCATGAC 
CAGCATGCAG TATTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTTTGGT CCATGCCACA 
GAGCGGGAGA GCAAGCCTCC TGTCCGAGAT ACCCGTGAGA GCCAGCTGGC ACACATCAAG 
GATGAACCTC CTCCACTCTC CCCCGCCCCC TTAACCCCAG CCACTCCATC CTCCCTTGAC 
CCATTCTTCT CCAGGGAGCC CTCATCTATG CACATTTCCT CGAGCCTGCC CCCTGACACA 
CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT ACTACGGCAG 
TCTACCACCC ATCTTGCTGA TGGGCCTTTT GCTGTCTTGG TAGATTATAT CCGTGTCCTC 
GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTGGA CGAAGGGCTC 
CGGAAAGAAG ATATGGCAGT GCATGTCCGC CGTGACCATG TATTTGAAGA CTCCTATCGT 
GAGCTGCATC GCAAATCTCC TGAAGAAATG AAGAATCGAT TGTATATAGT GTTTGAAGGA 
GAAGAAGGGC AGGATGCTGG GGGGCTCCTG CGAGAGTGGT ACATGATCAT CTCTCGAGAG 
ATGTTCAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGACCGGGT CACCTACACC 
ATCAATCCAT CTTCCCACTG TAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTTGGACGA 
ATTGTAGCCA AAGCTGTATA TGACAACCGC CTCCTGGAGT GCTACTTTAC TAGGTCTTTC 
TACAAACACA TCTTGGGCAA ATCTGTCAGG TATACAGATA TGGAGAGTGA AGATTACCAC 
TTCTACCAGG GCCTAGTTTA TCTGCTTGAA AATGATGTCT CTACATTAGG CTATGACCTC 
ACCTTCAGCA CTGAGGTTCA AGAATTTGGA GTATGTGAAG TTCGTGACCT CAAACCCAAT 
GGAGCCAACA TCTTGGTAAC AGAGGAGAAC AAGAAGGAAT ATGTACACCT GGTTTGCCAG 
ATGAGAATGA CAGGAGCCAT CCGCAAACAG CTGGCAGCCT TCTTGGAGGG CTTCTATGAA 
ATCATTCCAA AGCGCCTCAT ATCCATCTTT ACTGAGCAGG AATTAGAGCT GCTCATATCA 
GGGCTGCCTA CCATCGACAT TGATGATCTA AAATCTAACA CTGAGTACCA CAAGTACCAA 
TCCAACTCTA TTCAGATCCA GTGGTTTTGG AGAGCATTGC GTTCCTTTGA CCAAGCAGAC 
CGTGCCAAGT TCCTCCAGTT TGTCACAGGT ACTTCCAAGG TACCCCTACA AGGATTTGCT 
GCCCTTGAAG GTATGAATGG CATTCAGAAG TTTCAGATTC ATCGAGATGA CAGATCCACA 
GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAACTGG ACCTGCCTGC CTATGAGAGC 
TTTGAGAAGC TCCGCCACAT GCTACTATTG GCCATCCAGG AATGCTCTGA AGGCTTTGGG 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251445.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107305.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X21, mRNA.

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CloneID	OMu90433
Clone ID Related Accession (Same CDS sequence)	XM_030251445.1
Accession Version	XM_030251445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG ATGATGAATT TGAGAATCTT AGAATTAAAG GTCCTAATGC TGTACAGCTG 
GTAAAGACAA CACCTTTGAA GCCCTCATCT CTGCCTGTCA TTCCTGATAC TATTAAGGAA 
GTTATCTATG ATATGCTGAA TGCTTTGGCT GCATACCATG CTCCAGAGGA AGACAAATCT 
GATCCTAAAC CTGGGGGAAC GACCCAAGAG GTTGGCCAGC TCCTGCAAGA CATGGGTGAC 
GATGTATACC AGCAGTACCG GTCACTTACT CGTCAGAGCA GTGACTTTGA TACACAGTCA 
GGTTTTTCCT TAAATAGTCA GGTTTTTGCT GCAGATGGTG CCCCTGCTGA AACTTCCACA 
ACTGGAACCT CCCAAGGAGA GGGAGCTTCA ACTCCAGAGG AGACTCGAGA AGGGAAGAAA 
GATAAAGAAG GGGACCGTAC ATCTGAGGAA GGCAAGCAGA AAAGCAAGGG CAGCAAACCT 
TTAATGCCTA CCTCTACTAT CCTTCGTCTT CTGGCAGAGT TGGTGAGGTC CTATGTTGGT 
ATTGCTACCC TGATTGCAAA CTACAGCTAT ACCGTGGGCC AGTCTGAGCT GATCAAAGAG 
GACTGCAGTG TGCTAGCTTT TGTCCTGGAC CACCTCCTCC CACACACCCA GAATGCAGAA 
GACAAGGATA CACCTGCCCT GGCACGTCTA TTCCTTGCAA GTCTGGCTGC TGCGGGGAGT 
GGCACAGATG CTCAAGTGGC CCTAGTGAAT GAAGTGAAAG CAGCCCTGGG ACGGGCGCTT 
GCTATGGCTG AGAGTACAGA GAAGCACGCC AGACTTCAGG CAGTGATGTG TATCATCAGT 
ACTATCATGG AATCCTGCCC CTCCACCTCC AGCTTCTACA GTAGTGCTAC AGCCAAGACT 
CAGCATAATG GCATGAACAA CATCATTCGA CTGTTCCTAA AGAAGGGACT AGTCAATGAC 
CTGGCCAGAG TTCCTCACAG CCTAGACCTG TCCAGTCCCA ATATGGCCAA CACAGTCAAT 
GCTGCTCTAA AACCCTTGGA AACACTTTCC CGGATTGTAA ATCAACCCAG TAGCCTTTTT 
GGCAGCAAGA GTGCTTCTAG CAAGAACAAG TCTGAGCAGG ATGCTCAAGG AGCTTCTCAG 
GACTCCAGCA GCCATCAGCA AGATCCAGGC GAGCCTGGGG AGGCAGAAGT GCAGGAGGAG 
GATCATGATG TCACTCAGAC AGAGGTGGCA GATGGGGATA TCATGGATGG GGAGGCTGAA 
ACCGACTCAG TGGTGATTGC CGGGCAGCCT GAGGTGCTCA GTTCACAAGA GATGCAGGTT 
GAAAATGAAC TGGAGGACCT GATAGATGAG TTGCTTGAGA GGGATGGCGG ATCTGGGAAC 
AGTACAATTA TAGTGAGCAG AAGTGGAGAG GATGAATCAC AAGAGGACGT GCTGATGGAT 
GAAGCTCCTT CCAACCTCAG CCAAGCTTCC ACCTTGCAGG CCAACCGAGA AGATTCCATG 
AATATCCTGG ACCCTGAGGA TGAGGAGGAA CATACTCAGG AAGAGGACAG CAGTGGCAGT 
AACGAGGATG AGGATGATAG TCAGGATGAA GAGGAGGAGG AGGAGGAAGA TGAGGAAGAT 
GATCAGGAGG ATGATGAAGG TGAAGAAGGA GATGAAGATG ATGACGACGA TGGTTCTGAG 
ATGGAATTGG ATGAGGATTA CCCTGATATG AACGCTTCTC CCTTGGTCCG ATTTGAGCGC 
TTTGACCGGG AGGATGATCT CATCATTGAG TTTGACAACA TGTTCTCCAG TGCTACAGAC 
ATTCCCCCAT CCCCAGGAAA TATCCCTACC ACCCACCCAT TGATGGTACG TCATGCAGAT 
CACAGTTCCC TGACACTGGG CAGTGGTTCT TCCACAACTC GTCTTACCCA GGGCATCGGT 
CGCAGTCAAA GGACCCTCAG ACAGCTCACT GCCAATACAG GCCATACCAT TCATGTTCAC 
TACCCTGGGA ATCGCCAGCC TAACCCTCCT CTTATACTCC AGAGGTTGCT TGGTCCCTCT 
GCAGCTGCTG ACATTCTTCA GCTGAGCAGC AGCCTTCCAT TACAAAGCCG AGGCCGGGCC 
CGCCTCCTAG TGGGCAATGA TGACGTCCAT ATCATCGCCA GGTCTGATGA CGAGCTGCTG 
GATGACTTTT TTCATGACCA GAGTACAGCC ACTAGCCAAG CAGGAACCCT GTCCAGCATC 
CCAACAGCTC TAACCCGATG GACAGAGGAA TGCAAAGTTC TTGATGCTGA GAGTATGCAT 
GACTGTGTTT CAGTGGTTAA AGTGCCCATT GTCAATCACC TGGAATTCTT GAGAGATGAG 
GAGCTAGAAG AGAGAAGGGA GAAACGCAGG AAACAATTGG CTGAGGAAGA AACAAAAATA 
ATAGACAAAG GCAAAGAAGA CAAGGAGAAC AGAGATCAGA GTGCACAGTG TACTGTAACT 
AAGACAAATG ACTCCACTGA ACAGAATGTC TCAGATGGGA CTCCTATGCC TGACAGCTAT 
CCAACAACCC CCTCTTCAAC GGATGCACCT ACATCTGAGT CCAAGGAAAC ACTTGGTACC 
CTACAACCCT CCCAGCAACA GCCAGCACTT CCACCCCCAC CATCCTTAGG AGAGATTCCT 
CAGGAACTAC AGTCCCCTGC TGAAGAAGTG GCAAACTCTA CACAGCTATT GATGCCTATA 
GAGTTGGAGG AATTGGGTCC CACAAGACCA AGTGGAGAAG CAGAAACAAC TCAAATGGAA 
TTGTCTCCAG CTCCCACCAT AACCTCTCTT TCCCCAGAGA GAGCTGAAGA TTCAGACGCG 
TTAACAGCTG TCAGCAGTCA GCTGGAAGGT TCTCCCATGG ACACCAGCAG CCTGGCTTCC 
TGTACGTTAG AAGAGGCTGT GGGTGACACT CCAGCAGCTG GCAGTTCTGA GCAGCCCACA 
GCAGGTAGTT CCACTCCTGG GGATGCGCCA TCAGTTGTGG CAGAAGTGCA AGGCAGACCT 
GATGTATCAA GAGAATCCAA CCAGCCACCT GAGGACAGCT CCCCCCCTGC ATCCTCGGAG 
AGTTCTTCCA CTAGAGATTC TGCCGTGGCC ATCTCTGGAG CAGATTCTCG TGGAATTCTA 
GAAGAGCCAC TACCTTCAAC AAGCAGTGAA GAAGAAGATC CCCTTGCTGG TATCAGTCTC 
CCTGAAGGTG TGGATCCCTC CTTTCTGGCT GCTCTCCCTG ATGATATCCG TCGGGAAGTC 
CTACAGAACC AGCTGGGCAT ACGTCCACCA ACCCGAAGTG CTCCCTCCTC AAATAGCTCA 
GCTCCTGCAG TAGTGGGGAA CCCTGGTGTA ACTGAAGTGA GCCCTGAGTT CCTGGCTGCC 
CTACCTCCAG CTATTCAAGA GGAAGTGCTA GCACAGCAGA GAGCTGAACA GCAGCGACGG 
GAACTGGCTC AGAATGCGAG CTCAGACACC CCCATGGACC CTGTGACTTT CATTCAGACT 
TTGCCCTCAG ATCTGCGCCG TAGTGTCCTA GAGGATATGG AAGACAGTGT GTTAGCTGTG 
ATGCCACCTG ACATTGCAGC TGAGGCTCAA GCTCTAAGAC GAGAACAAGA AGCCCGACAG 
CGGCAGCTCA TGCATGAGCG TCTTTTTGGA CACAGTAGTA CTTCTGCACT CTCTGCCATT 
CTCCGAAGCC CGGCTTTTAC CAGTCGCCTG AGTGGCAACC GTGGGGTCCA GTATACTCGC 
CTTGCTGTAC AGAGGGGTGG CACCTTCCAG ATGGGGGGTA GCAGCAGCCA TAATAGGCCT 
TCTGGCAGTA ATGTGGACAC ACTACTCCGC CTCCGAGGAC GACTCCTTCT AGACCATGAA 
GCCCTGTCTT GCCTCTTGGT CCTACTTTTT GTGGATGAGC CAAAGCTCAA TACTAGCCGT 
CTACACCGAG TACTGAGAAA TCTCTGCTAT CATGCCCAGA CCCGCCACTG GGTCATCCGC 
AGCCTCCTGT CCATTTTGCA GCGCAGCAGT GAAAGTGAGT TATGCATTGA GACACCTAAG 
CTCTCCACAA GTGAAGAAAG GGGCAAGAAA TCAAGCAAGA GCTGTGCATC AAGCAGTCAT 
GAGAACCGTC CCCTGGATCT GCTCCACAAG ATGGAGTCCA AAAGTTCCAA TCAGCTCTCC 
TGGCTCTCAG TATCAATGGA TGCTGCTTTA GGCTGTAGGA CTAACATATT CCAGATTCAA 
CGTTCTGGAG GGCGTAAACA TACAGAGAAG CATGCAAGCA GTGGTTCCAC TGTCCACATC 
CACCCCCAGG CTGCTCCTGT TGTTTGCAGA CATGTTTTGG ATACACTCAT TCAGTTGGCC 
AAGGTGTTTC CCAGCCACTT TACACAGCAG CGGACCAAAG AAACAAACTG TGAGAGTGAT 
CGGGAACGGG GCAGTAAGCA GGCCTGCAGC CCATGCTCCT CACAGTCCTC CAGCAGTGGC 
ATCTGTACAG ACTTCTGGGA CTTATTGGTA AAACTGGACA ACATGAATGT TAGCCGAAAA 
GGCAAGAACT CCGTGAAGTC AGTACCAGTG AGCTCTGGTG GTGAAGGGGA AACTTCCCCA 
CACAGCCTTG AAGCCTCTCC ACTGGGGCAG CTCATGAACA TGTTGTCACA TCCAGTCATC 
CGCCGGAGTT CTCTCTTAAC TGAGAAACTC CTGAGACTCC TCTCTCTCAT CTCAATTGCT 
CTCCCAGAAA ACAAAGTATC AGAAGTGCAA ACTAACTCCA GCAACAGTGG TTCCTCCACT 
GCTGCCACCT CAAACACATC TACTACTACC ACCACCACCA CTACTGCCAC TGCCCCCACG 
CCCACACCCC CAGCTGCAAC TACCCCTGTC ACTTCTGCTC CAGCTCTGGT TGCTGCCACA 
GCTATTTCCA CCATTACTGT AGCTGCTTCT ACCACAGTGA CTACCCCCAC AACTGCTACC 
ACTACTGTTT CAACTTCTAC TACTAAAGGC AGCAAATCTC CAGCAAAGGT TGGTGAAGGA 
GGCAGTGGTA TAGACTTCAA AATGGTGTCC TCTGGCCTCA CTGAAAACCA GCTACAGCTC 
TCTGTAGAGG TGTTGACATC CCACTCTTGT TCTGAAGAAG GCTTGGAAGA TGCGGCCAAT 
GTGTTACTGC AGCTCTCTCG TGGGGACTCT GGTACCCGGG ACACTGTTCT TAAGCTGCTA 
CTAAATGGAG CCCGCCATCT GGGTTATACC CTCTGTAAAC AGATAGGTAC CCTACTCGCT 
GAACTACGGG AATATAATCT GGAGCAGCAG CGGCGAGCCC AATGCGAGAC CCTCTCTCCT 
GATGGCCTAC CTGAGGAGCA ACCACAGACC ACCAAACTGA AAGGCAAAAT GCAGAGCAGG 
TTTGACATGG CTGAAAATGT GGTAATTGTG GCATCTCAGA AGCGACCCTT GGGTGGCCGG 
GAGCTCCAGC TGCCTTCTAT GTCCATGTTG ACATCCAAGA CATCTACCCA GAAGTTCTTC 
TTGAGGGTAC TACAGGTCAT CATCCAGCTG CGGGATGACA CACGACGAGC TAACAAGAAA 
GCCAAGCAGA CAGGCAGGCT AGGTTCCTCC GGTTTAGGCT CAGCTAGCAG CATCCAGGCA 
GCTGTTCGGC AGCTGGAGGC TGAGGCTGAT GCCATTATAC AAATGGTACG TGAGGGTCAA 
AGGGCGCGGA GACAGCAACA AGCAGCAACG TCGGAGTCTT CTAACCAGTC AGAGACATCT 
GTCCGGAGAG AGGAATCGCC CATGGATGTG GACCAGCCAT CTCCTAGTGC TCAAGATACT 
CAGTCCATTG TAATCTCAGA TGGAACACCA CAGGGAGAGA AGGAGAAAGA GGAGAAACCA 
CCTGAGTTAC CCCTGCTCAG TGAGCAACTG AGCCTAGATG AACTGTGGGA CATGCTAGGG 
GAATGTTTAA AAGAACTGGA GGAATCTCAT GACCAGCATG CAGTATTAGT GCTACAGCCT 
GCTGTCGAGG CCTTCTTTTT GGTCCATGCC ACAGAGCGGG AGAGCAAGCC TCCTGTCCGA 
GATACCCGTG AGAGCCAGCT GGCACACATC AAGGATGAAC CTCCTCCACT CTCCCCCGCC 
CCCTTAACCC CAGCCACTCC ATCCTCCCTT GACCCATTCT TCTCCAGGGA GCCCTCATCT 
ATGCACATTT CCTCGAGCCT GCCCCCTGAC ACACAGAAGT TCCTTCGCTT TGCAGAGACT 
CACCGCACTG TGTTAAACCA GATACTACGG CAGTCTACCA CCCATCTTGC TGATGGGCCT 
TTTGCTGTCT TGGTAGATTA TATCCGTGTC CTCGACTTTG ATGTCAAGCG CAAATATTTC 
CGCCAAGAGC TGGAGCGTTT GGACGAAGGG CTCCGGAAAG AAGATATGGC AGTGCATGTC 
CGCCGTGACC ATGTATTTGA AGACTCCTAT CGTGAGCTGC ATCGCAAATC TCCTGAAGAA 
ATGAAGAATC GATTGTATAT AGTGTTTGAA GGAGAAGAAG GGCAGGATGC TGGGGGGCTC 
CTGCGAGAGT GGTACATGAT CATCTCTCGA GAGATGTTCA ACCCTATGTA TGCCTTGTTC 
CGTACCTCAC CTGGTGACCG GGTCACCTAC ACCATCAATC CATCTTCCCA CTGTAACCCC 
AACCACCTCA GCTACTTCAA GTTTGTTGGA CGAATTGTAG CCAAAGCTGT ATATGACAAC 
CGCCTCCTGG AGTGCTACTT TACTAGGTCT TTCTACAAAC ACATCTTGGG CAAATCTGTC 
AGGTATACAG ATATGGAGAG TGAAGATTAC CACTTCTACC AGGGCCTAGT TTATCTGCTT 
GAAAATGATG TCTCTACATT AGGCTATGAC CTCACCTTCA GCACTGAGGT TCAAGAATTT 
GGAGTATGTG AAGTTCGTGA CCTCAAACCC AATGGAGCCA ACATCTTGGT AACAGAGGAG 
AACAAGAAGG AATATGTACA CCTGGTTTGC CAGATGAGAA TGACAGGAGC CATCCGCAAA 
CAGCTGGCAG CCTTCTTGGA GGGCTTCTAT GAAATCATTC CAAAGCGCCT CATATCCATC 
TTTACTGAGC AGGAATTAGA GCTGCTCATA TCAGGGCTGC CTACCATCGA CATTGATGAT 
CTAAAATCTA ACACTGAGTA CCACAAGTAC CAATCCAACT CTATTCAGAT CCAGTGGTTT 
TGGAGAGCAT TGCGTTCCTT TGACCAAGCA GACCGTGCCA AGTTCCTCCA GTTTGTCACA 
GGTACTTCCA AGGTACCCCT ACAAGGATTT GCTGCCCTTG AAGGTATGAA TGGCATTCAG 
AAGTTTCAGA TTCATCGAGA TGACAGATCC ACAGATCGCC TGCCTTCAGC TCACACATGT 
TTTAATCAAC TGGACCTGCC TGCCTATGAG AGCTTTGAGA AGCTCCGCCA CATGCTACTA 
TTGGCCATCC AGGAATGCTC TGAAGGCTTT GGGCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107305.1
CDS	1856..15217
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDVADVTMESSPGSSISMEHRLDVELRASSSNSSTSISGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEDKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251445.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251445.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTA 
ACTATGGAAA GCAGTCCAGG CTCGTCCATA TCTATGGAGC ACCGGCTGGA TGTTGAATTA 
AGGGCATCAA GTTCCAACAG CAGCACTAGC ATCTCTGGCC CTGGCCCGGG TCCTGGCCCT 
GGTCCGGGTC CTGGCCCTGG TCCTGGCCCT GGTCCTGGTC CTGGCCCTGG CCCTGGCCCT 
GGCCCCGGCC CTGGCCCTGG TCCTGGCCCT GGACCCCGTC CTGGAGTTCA GTGTATTCCA 
CAACGAGCAG CACTTCTCAA GTCCATGTTG AATTTCCTCA AGAAGGCCAT TCAAGACCCT 
GCTTTCTCAG ATGGCATACG ACATGTGATG GATGGTTCTT TACCTACCTC TCTGAAACAC 
ATCATCAGCA ATGCAGAATA CTATGGTCCA TCACTCTTCC TCCTAGCTAC TGAAGTGGTG 
ACTGTGTTTG TATTTCAAGA GCCATCACTG CTGTCCTCAC TCCAGGACAA TGGACTGACA 
GATGTCATGC TGCATGCACT GCTCATCAAA GATGTTCCTG CTACAAGGGA AGTCCTCGGT 
TCCCTCCCAA ATGTATTCAG TGCACTTTGC TTGAATGCCC GAGGTCTTCA GTCTTTTGTA 
CAATGTCAGC CATTTGAACG TCTCTTCAAA GTTCTTCTGT CTCCTGATTA CCTCCCAGCC 
ATGCGGAGGA GGAGGAGTTC TGATCCCCTT GGGGATACTG CATCCAACCT GGGGAGTGCT 
GTTGATGAGC TCATGAGACA CCAGCCTACC CTCAAAACAG ATGCAACAAC TGCCATCATC 
AAGTTACTTG AAGAAATCTG TAATCTTGGA AGAGATCCTA AATACATTTG TCAGAAGCCA 
TCTATTCAGA AAGCGGATGG TACTGCTACT GCTCCTCCTC CAAGGTCTAA TCATGCTGCA 
GAAGAGGCTT CCAGTGAAGA TGAGGAGGAA GAGGAAGTAC AGGCCATGCA GAGCTTTAAT 
TCTGCCCAGC AGAATGAAAC TGAGCCTAAT CAGCAGGTCG TTGGTACCGA GGAACGTATA 
CCTATACCCC TCATGGATTA CATCCTTAAT GTGATGAAAT TTGTGGAATC TATTTTGAGC 
AACAATACAA CAGATGACCA CTGTCAAGAA TTTGTAAACC AGAAAGGACT CTTACCTTTG 
GTTACCATTT TGGGTCTTCC CAATCTGCCC ATTGACTTTC CCACATCTGC TGCCTGTCAG 
GCTGTTGCAG GTGTCTGCAA ATCCATATTG ACCCTGTCAC ATGAACCCAA AGTTCTTCAA 
GAAGGTCTCC TTCAATTAGA CTTGATCCTT TCCTCCTTGG AGCCTTTACA TCGGCCGATT 
GAATCCCCTG GGGGCTCAGT GTTACTGAGA GAATTGGCAT GTGCAGGCAA TGTTGCTGAT 
GCTACCCTCT CAGCTCAGGC CACGCCTCTG TTGCATGCAC TCACTGCAGC CCATGCTTAC 
ATCATGATGT TTGTTCACAC TTGCAGAGTT GGACAGAGTG AAATTCGTTC TATCTCAGTA 
AACCAGTGGG GTTCACAATT GGGTTTGAGT GTTCTGAGCA AGCTAAGCCA GTTATACTGT 
TCTCTGGTGT GGGAAAGCAC TGTCCTCCTC TCTCTATGTA CCCCCAACAG CCTACCATCT 
GGATGTGAAT TTGGTCAAGC GGACATGCAG AAACTAGTTC CAAAGGATGA GAAGGCAGGT 
ACGACCCAGG GCGGAAAAAG ATCAGATGGA GAACAGGATG GAACTGCTGG AAGCATGGAT 
GCATCTGCCC AGGGTTTATT AGAAGGAATT GAACTAGATG GTGACACACT GGCACCCATG 
GAGACAGATG AACCTTCTTC TTCAGATTCT AAGGGCAAAT CTAAAATCAC ACCTGCCATG 
GCTGCAAGAA TTAAACAAAT CAAGCCGTTG CTGTCAGCTT CTTCCAGATT AGGCCGAGCA 
CTTGCTGAGC TCTTTGGACT CCTTGTTAAG CTTTGTGTGG GTTCTCCTGT CCGCCAAAGG 
AGGAGCCATC ATGCTGCCAG TACCACCACT GCACCAACAC CTGCTGCTAG ATCAACAGCT 
TCAGCCCTCA CTAAGCTACT CACAAAAGGC TTATCATGGC AGCCCCCACC ATATACACCC 
ACTCCCCGCT TCAGGCTGAC GTTCTTCATC TGTTCAGTTG GATTCACCTC TCCAATGCTC 
TTTGATGAGA GAAAGTATCC TTACCACCTT ATGCTGCAGA AATTTCTCTG CTCTGGTGGT 
CACAATGCTC TTTTTGAAAC TTTCAACTGG GCTCTATCCA TGGGAGGAAA AGTTCCTGTT 
TCTGAGGGAT TGGAACATTC AGATCTACCT GATGGTACAG GAGAATTCCT AGATGCCTGG 
CTTATGCTTG TAGAAAAGAT GGTAAATCCC ACCACGGTGC TTGAATCTCC ACATTCGTTG 
CCTGCTAAAT TGCCTGGAGG AGTCCAGAGC TTTCCACAAT TTAGTGCCCT TCGCTTCCTT 
GTAGTAACTC AAAAAGCAGC CTTTACTTGT ATCAAGAACC TGTGGAACCG GAAACCCCTT 
AAAGTATATG GGGGAAGAAT GGCTGAGTCT ATGCTGGCCA TTTTGTGCCA CATTCTCCGA 
GGGGAACCTG TGATTCGAGA AAGACTGAGC AAAGAGAAGG AAGGATCTCG AGGAGAAGAA 
GAAGCAGGCC AGGAAGAAGG TGGCTCACGC AGAGAACCTC AAGTCAACCA GCAACAGCTA 
CAACAGCTCA TGGACATGGG TTTCACAAGA GAACATGCCA TGGAGGCACT GTTGAATACC 
AGTACGATGG AACAAGCAAC AGAGTACCTT TTAACCCACC CTCCTCCAAT CATAGGAGGA 
GTTGTTCGGG ATCTTAGCAT GTCAGAAGAG GACCAGATGA TGAGAGCAAT TGCTATGTCT 
CTGGGACAGG ATATTCCAAT GGATCAAAGG GCAGAGTCAC CTGAGGAAGT TGCTTGCCGG 
AAGGAGGAAG AAGAGCGTAA AGCTCGAGAA AAACAAGAAG AGGAAGAAGC TAAATGTCTA 
GAGAAGTTTC AAGATGCTGA CCCCTTGGAA CAAGATGAGC TCCACACTTT CACAGATACA 
ATGTTACCAG GCTGCTTCCA TCTTCTTGAT GAGTTGCCTG ATACAGTGTA CCGTGTTTGT 
GATCTGATCA TGACAGCTAT CAAACGTAAT GGAGCTGACT ATCGTGATAT GATTCTAAAG 
CAAGTTGTCA ACCAGGTGTG GGAAGCTGCT GATGTATTGA TCAAAGCTGC TCTTCCTCTA 
ACAACAAGTG ACACAAAAAC AGTTTCAGAG TGGATCAGTC AGATGGCTAC CCTGCCACAG 
GCCTCCAATT TGGCTACTAG AATCTTGCTT TTAACACTAC TTTTTGAGGA GTTGAAGTTA 
CCTTGTGCCT GGGTGGTTGA ATCAAGTGGC ATTCTTAATG TCCTAATCAA ACTCTTAGAA 
GTGGTTCAAC CCTGCCTACA GGCTGCCAAA GAGCAAAAGG AAGTCCAGAC CCCAAAGTGG 
ATCACCCCAG TGTTGCTCCT TATTGATTTT TATGAAAAGA CAGCCATCTC ATCAAAGAGA 
AGAGCCCAAA TGACTAAGTA CCTACAGTCC AACAGCAACA ACTGGCGCTG GTTTGATGAC 
CGCTCTGGGC GTTGGTGTAG TTACAGTGCT AGCAACAACA GCACCATTGA TTCTGCCTGG 
AAATCTGGAG AAACAAGTGT AAGATTCACT GCTGGCCGAC GAAGATACAC TGTCCAGTTC 
ACTACAATGG TGCAGGTTAA TGAAGAAACA GGAAACCGAC GCCCTGTGAT GCTGACTCTC 
CTCAGGGTGC CACGACTGAG TAAGAACTCA AAAAGCAGCA ATGGACAGGA ACTAGAGAAG 
ACACTGGAAG AAAGCAAGGA GACCGATATC AAACATAAAG AAAATAAAGG CAATGATATC 
CCCTTAGCTC TAGAGAGCAC AAACACTGAA AAAGAGGCAA GCCTGGATGA AACAAAAATT 
GGGGAGATCC TAATCCAGGG CCTGACAGAA GATATGGTGA CTGTTCTAAT CCGTGCCTGT 
GTGAGCATGC TGGGAGTCCC TGTGGACCCA GACACTTTGC ATGCCACCCT TCGTCTCTGT 
TTAAGGCTCA CCCGAGACCA CAAATATGCC ATGATGTTTG CAGAACTGAA GAGTACACGA 
ATGATCTTGA ATTTGACCCA GAGCTCAGGT TTCAATGGGT TTACCCCCTT AGTCACCCTT 
CTCTTAAGGC ACATCATTGA GGATCCTTGT ACTCTTCGTC ATACTATGGA AAAGGTTGTT 
CGCTCGGCAG CCACAAGTGG AGCTGGTAGT ACTACATCTG GTGTTGTATC TGGCAGCCTC 
GGTTCTCGGG AGATCAACTA TATCCTTAGA GTCCTAGGAC CAGCTGCCTG CCGCAATCCA 
GACATATTCA CAGAAGTAGC CAATTGCTGT ATCCGCATTG CCCTTCCAGC CCCTCGAGGC 
TCAGGAACTG ATGATGAATT TGAGAATCTT AGAATTAAAG GTCCTAATGC TGTACAGCTG 
GTAAAGACAA CACCTTTGAA GCCCTCATCT CTGCCTGTCA TTCCTGATAC TATTAAGGAA 
GTTATCTATG ATATGCTGAA TGCTTTGGCT GCATACCATG CTCCAGAGGA AGACAAATCT 
GATCCTAAAC CTGGGGGAAC GACCCAAGAG GTTGGCCAGC TCCTGCAAGA CATGGGTGAC 
GATGTATACC AGCAGTACCG GTCACTTACT CGTCAGAGCA GTGACTTTGA TACACAGTCA 
GGTTTTTCCT TAAATAGTCA GGTTTTTGCT GCAGATGGTG CCCCTGCTGA AACTTCCACA 
ACTGGAACCT CCCAAGGAGA GGGAGCTTCA ACTCCAGAGG AGACTCGAGA AGGGAAGAAA 
GATAAAGAAG GGGACCGTAC ATCTGAGGAA GGCAAGCAGA AAAGCAAGGG CAGCAAACCT 
TTAATGCCTA CCTCTACTAT CCTTCGTCTT CTGGCAGAGT TGGTGAGGTC CTATGTTGGT 
ATTGCTACCC TGATTGCAAA CTACAGCTAT ACCGTGGGCC AGTCTGAGCT GATCAAAGAG 
GACTGCAGTG TGCTAGCTTT TGTCCTGGAC CACCTCCTCC CACACACCCA GAATGCAGAA 
GACAAGGATA CACCTGCCCT GGCACGTCTA TTCCTTGCAA GTCTGGCTGC TGCGGGGAGT 
GGCACAGATG CTCAAGTGGC CCTAGTGAAT GAAGTGAAAG CAGCCCTGGG ACGGGCGCTT 
GCTATGGCTG AGAGTACAGA GAAGCACGCC AGACTTCAGG CAGTGATGTG TATCATCAGT 
ACTATCATGG AATCCTGCCC CTCCACCTCC AGCTTCTACA GTAGTGCTAC AGCCAAGACT 
CAGCATAATG GCATGAACAA CATCATTCGA CTGTTCCTAA AGAAGGGACT AGTCAATGAC 
CTGGCCAGAG TTCCTCACAG CCTAGACCTG TCCAGTCCCA ATATGGCCAA CACAGTCAAT 
GCTGCTCTAA AACCCTTGGA AACACTTTCC CGGATTGTAA ATCAACCCAG TAGCCTTTTT 
GGCAGCAAGA GTGCTTCTAG CAAGAACAAG TCTGAGCAGG ATGCTCAAGG AGCTTCTCAG 
GACTCCAGCA GCCATCAGCA AGATCCAGGC GAGCCTGGGG AGGCAGAAGT GCAGGAGGAG 
GATCATGATG TCACTCAGAC AGAGGTGGCA GATGGGGATA TCATGGATGG GGAGGCTGAA 
ACCGACTCAG TGGTGATTGC CGGGCAGCCT GAGGTGCTCA GTTCACAAGA GATGCAGGTT 
GAAAATGAAC TGGAGGACCT GATAGATGAG TTGCTTGAGA GGGATGGCGG ATCTGGGAAC 
AGTACAATTA TAGTGAGCAG AAGTGGAGAG GATGAATCAC AAGAGGACGT GCTGATGGAT 
GAAGCTCCTT CCAACCTCAG CCAAGCTTCC ACCTTGCAGG CCAACCGAGA AGATTCCATG 
AATATCCTGG ACCCTGAGGA TGAGGAGGAA CATACTCAGG AAGAGGACAG CAGTGGCAGT 
AACGAGGATG AGGATGATAG TCAGGATGAA GAGGAGGAGG AGGAGGAAGA TGAGGAAGAT 
GATCAGGAGG ATGATGAAGG TGAAGAAGGA GATGAAGATG ATGACGACGA TGGTTCTGAG 
ATGGAATTGG ATGAGGATTA CCCTGATATG AACGCTTCTC CCTTGGTCCG ATTTGAGCGC 
TTTGACCGGG AGGATGATCT CATCATTGAG TTTGACAACA TGTTCTCCAG TGCTACAGAC 
ATTCCCCCAT CCCCAGGAAA TATCCCTACC ACCCACCCAT TGATGGTACG TCATGCAGAT 
CACAGTTCCC TGACACTGGG CAGTGGTTCT TCCACAACTC GTCTTACCCA GGGCATCGGT 
CGCAGTCAAA GGACCCTCAG ACAGCTCACT GCCAATACAG GCCATACCAT TCATGTTCAC 
TACCCTGGGA ATCGCCAGCC TAACCCTCCT CTTATACTCC AGAGGTTGCT TGGTCCCTCT 
GCAGCTGCTG ACATTCTTCA GCTGAGCAGC AGCCTTCCAT TACAAAGCCG AGGCCGGGCC 
CGCCTCCTAG TGGGCAATGA TGACGTCCAT ATCATCGCCA GGTCTGATGA CGAGCTGCTG 
GATGACTTTT TTCATGACCA GAGTACAGCC ACTAGCCAAG CAGGAACCCT GTCCAGCATC 
CCAACAGCTC TAACCCGATG GACAGAGGAA TGCAAAGTTC TTGATGCTGA GAGTATGCAT 
GACTGTGTTT CAGTGGTTAA AGTGCCCATT GTCAATCACC TGGAATTCTT GAGAGATGAG 
GAGCTAGAAG AGAGAAGGGA GAAACGCAGG AAACAATTGG CTGAGGAAGA AACAAAAATA 
ATAGACAAAG GCAAAGAAGA CAAGGAGAAC AGAGATCAGA GTGCACAGTG TACTGTAACT 
AAGACAAATG ACTCCACTGA ACAGAATGTC TCAGATGGGA CTCCTATGCC TGACAGCTAT 
CCAACAACCC CCTCTTCAAC GGATGCACCT ACATCTGAGT CCAAGGAAAC ACTTGGTACC 
CTACAACCCT CCCAGCAACA GCCAGCACTT CCACCCCCAC CATCCTTAGG AGAGATTCCT 
CAGGAACTAC AGTCCCCTGC TGAAGAAGTG GCAAACTCTA CACAGCTATT GATGCCTATA 
GAGTTGGAGG AATTGGGTCC CACAAGACCA AGTGGAGAAG CAGAAACAAC TCAAATGGAA 
TTGTCTCCAG CTCCCACCAT AACCTCTCTT TCCCCAGAGA GAGCTGAAGA TTCAGACGCG 
TTAACAGCTG TCAGCAGTCA GCTGGAAGGT TCTCCCATGG ACACCAGCAG CCTGGCTTCC 
TGTACGTTAG AAGAGGCTGT GGGTGACACT CCAGCAGCTG GCAGTTCTGA GCAGCCCACA 
GCAGGTAGTT CCACTCCTGG GGATGCGCCA TCAGTTGTGG CAGAAGTGCA AGGCAGACCT 
GATGTATCAA GAGAATCCAA CCAGCCACCT GAGGACAGCT CCCCCCCTGC ATCCTCGGAG 
AGTTCTTCCA CTAGAGATTC TGCCGTGGCC ATCTCTGGAG CAGATTCTCG TGGAATTCTA 
GAAGAGCCAC TACCTTCAAC AAGCAGTGAA GAAGAAGATC CCCTTGCTGG TATCAGTCTC 
CCTGAAGGTG TGGATCCCTC CTTTCTGGCT GCTCTCCCTG ATGATATCCG TCGGGAAGTC 
CTACAGAACC AGCTGGGCAT ACGTCCACCA ACCCGAAGTG CTCCCTCCTC AAATAGCTCA 
GCTCCTGCAG TAGTGGGGAA CCCTGGTGTA ACTGAAGTGA GCCCTGAGTT CCTGGCTGCC 
CTACCTCCAG CTATTCAAGA GGAAGTGCTA GCACAGCAGA GAGCTGAACA GCAGCGACGG 
GAACTGGCTC AGAATGCGAG CTCAGACACC CCCATGGACC CTGTGACTTT CATTCAGACT 
TTGCCCTCAG ATCTGCGCCG TAGTGTCCTA GAGGATATGG AAGACAGTGT GTTAGCTGTG 
ATGCCACCTG ACATTGCAGC TGAGGCTCAA GCTCTAAGAC GAGAACAAGA AGCCCGACAG 
CGGCAGCTCA TGCATGAGCG TCTTTTTGGA CACAGTAGTA CTTCTGCACT CTCTGCCATT 
CTCCGAAGCC CGGCTTTTAC CAGTCGCCTG AGTGGCAACC GTGGGGTCCA GTATACTCGC 
CTTGCTGTAC AGAGGGGTGG CACCTTCCAG ATGGGGGGTA GCAGCAGCCA TAATAGGCCT 
TCTGGCAGTA ATGTGGACAC ACTACTCCGC CTCCGAGGAC GACTCCTTCT AGACCATGAA 
GCCCTGTCTT GCCTCTTGGT CCTACTTTTT GTGGATGAGC CAAAGCTCAA TACTAGCCGT 
CTACACCGAG TACTGAGAAA TCTCTGCTAT CATGCCCAGA CCCGCCACTG GGTCATCCGC 
AGCCTCCTGT CCATTTTGCA GCGCAGCAGT GAAAGTGAGT TATGCATTGA GACACCTAAG 
CTCTCCACAA GTGAAGAAAG GGGCAAGAAA TCAAGCAAGA GCTGTGCATC AAGCAGTCAT 
GAGAACCGTC CCCTGGATCT GCTCCACAAG ATGGAGTCCA AAAGTTCCAA TCAGCTCTCC 
TGGCTCTCAG TATCAATGGA TGCTGCTTTA GGCTGTAGGA CTAACATATT CCAGATTCAA 
CGTTCTGGAG GGCGTAAACA TACAGAGAAG CATGCAAGCA GTGGTTCCAC TGTCCACATC 
CACCCCCAGG CTGCTCCTGT TGTTTGCAGA CATGTTTTGG ATACACTCAT TCAGTTGGCC 
AAGGTGTTTC CCAGCCACTT TACACAGCAG CGGACCAAAG AAACAAACTG TGAGAGTGAT 
CGGGAACGGG GCAGTAAGCA GGCCTGCAGC CCATGCTCCT CACAGTCCTC CAGCAGTGGC 
ATCTGTACAG ACTTCTGGGA CTTATTGGTA AAACTGGACA ACATGAATGT TAGCCGAAAA 
GGCAAGAACT CCGTGAAGTC AGTACCAGTG AGCTCTGGTG GTGAAGGGGA AACTTCCCCA 
CACAGCCTTG AAGCCTCTCC ACTGGGGCAG CTCATGAACA TGTTGTCACA TCCAGTCATC 
CGCCGGAGTT CTCTCTTAAC TGAGAAACTC CTGAGACTCC TCTCTCTCAT CTCAATTGCT 
CTCCCAGAAA ACAAAGTATC AGAAGTGCAA ACTAACTCCA GCAACAGTGG TTCCTCCACT 
GCTGCCACCT CAAACACATC TACTACTACC ACCACCACCA CTACTGCCAC TGCCCCCACG 
CCCACACCCC CAGCTGCAAC TACCCCTGTC ACTTCTGCTC CAGCTCTGGT TGCTGCCACA 
GCTATTTCCA CCATTACTGT AGCTGCTTCT ACCACAGTGA CTACCCCCAC AACTGCTACC 
ACTACTGTTT CAACTTCTAC TACTAAAGGC AGCAAATCTC CAGCAAAGGT TGGTGAAGGA 
GGCAGTGGTA TAGACTTCAA AATGGTGTCC TCTGGCCTCA CTGAAAACCA GCTACAGCTC 
TCTGTAGAGG TGTTGACATC CCACTCTTGT TCTGAAGAAG GCTTGGAAGA TGCGGCCAAT 
GTGTTACTGC AGCTCTCTCG TGGGGACTCT GGTACCCGGG ACACTGTTCT TAAGCTGCTA 
CTAAATGGAG CCCGCCATCT GGGTTATACC CTCTGTAAAC AGATAGGTAC CCTACTCGCT 
GAACTACGGG AATATAATCT GGAGCAGCAG CGGCGAGCCC AATGCGAGAC CCTCTCTCCT 
GATGGCCTAC CTGAGGAGCA ACCACAGACC ACCAAACTGA AAGGCAAAAT GCAGAGCAGG 
TTTGACATGG CTGAAAATGT GGTAATTGTG GCATCTCAGA AGCGACCCTT GGGTGGCCGG 
GAGCTCCAGC TGCCTTCTAT GTCCATGTTG ACATCCAAGA CATCTACCCA GAAGTTCTTC 
TTGAGGGTAC TACAGGTCAT CATCCAGCTG CGGGATGACA CACGACGAGC TAACAAGAAA 
GCCAAGCAGA CAGGCAGGCT AGGTTCCTCC GGTTTAGGCT CAGCTAGCAG CATCCAGGCA 
GCTGTTCGGC AGCTGGAGGC TGAGGCTGAT GCCATTATAC AAATGGTACG TGAGGGTCAA 
AGGGCGCGGA GACAGCAACA AGCAGCAACG TCGGAGTCTT CTAACCAGTC AGAGACATCT 
GTCCGGAGAG AGGAATCGCC CATGGATGTG GACCAGCCAT CTCCTAGTGC TCAAGATACT 
CAGTCCATTG TAATCTCAGA TGGAACACCA CAGGGAGAGA AGGAGAAAGA GGAGAAACCA 
CCTGAGTTAC CCCTGCTCAG TGAGCAACTG AGCCTAGATG AACTGTGGGA CATGCTAGGG 
GAATGTTTAA AAGAACTGGA GGAATCTCAT GACCAGCATG CAGTATTAGT GCTACAGCCT 
GCTGTCGAGG CCTTCTTTTT GGTCCATGCC ACAGAGCGGG AGAGCAAGCC TCCTGTCCGA 
GATACCCGTG AGAGCCAGCT GGCACACATC AAGGATGAAC CTCCTCCACT CTCCCCCGCC 
CCCTTAACCC CAGCCACTCC ATCCTCCCTT GACCCATTCT TCTCCAGGGA GCCCTCATCT 
ATGCACATTT CCTCGAGCCT GCCCCCTGAC ACACAGAAGT TCCTTCGCTT TGCAGAGACT 
CACCGCACTG TGTTAAACCA GATACTACGG CAGTCTACCA CCCATCTTGC TGATGGGCCT 
TTTGCTGTCT TGGTAGATTA TATCCGTGTC CTCGACTTTG ATGTCAAGCG CAAATATTTC 
CGCCAAGAGC TGGAGCGTTT GGACGAAGGG CTCCGGAAAG AAGATATGGC AGTGCATGTC 
CGCCGTGACC ATGTATTTGA AGACTCCTAT CGTGAGCTGC ATCGCAAATC TCCTGAAGAA 
ATGAAGAATC GATTGTATAT AGTGTTTGAA GGAGAAGAAG GGCAGGATGC TGGGGGGCTC 
CTGCGAGAGT GGTACATGAT CATCTCTCGA GAGATGTTCA ACCCTATGTA TGCCTTGTTC 
CGTACCTCAC CTGGTGACCG GGTCACCTAC ACCATCAATC CATCTTCCCA CTGTAACCCC 
AACCACCTCA GCTACTTCAA GTTTGTTGGA CGAATTGTAG CCAAAGCTGT ATATGACAAC 
CGCCTCCTGG AGTGCTACTT TACTAGGTCT TTCTACAAAC ACATCTTGGG CAAATCTGTC 
AGGTATACAG ATATGGAGAG TGAAGATTAC CACTTCTACC AGGGCCTAGT TTATCTGCTT 
GAAAATGATG TCTCTACATT AGGCTATGAC CTCACCTTCA GCACTGAGGT TCAAGAATTT 
GGAGTATGTG AAGTTCGTGA CCTCAAACCC AATGGAGCCA ACATCTTGGT AACAGAGGAG 
AACAAGAAGG AATATGTACA CCTGGTTTGC CAGATGAGAA TGACAGGAGC CATCCGCAAA 
CAGCTGGCAG CCTTCTTGGA GGGCTTCTAT GAAATCATTC CAAAGCGCCT CATATCCATC 
TTTACTGAGC AGGAATTAGA GCTGCTCATA TCAGGGCTGC CTACCATCGA CATTGATGAT 
CTAAAATCTA ACACTGAGTA CCACAAGTAC CAATCCAACT CTATTCAGAT CCAGTGGTTT 
TGGAGAGCAT TGCGTTCCTT TGACCAAGCA GACCGTGCCA AGTTCCTCCA GTTTGTCACA 
GGTACTTCCA AGGTACCCCT ACAAGGATTT GCTGCCCTTG AAGGTATGAA TGGCATTCAG 
AAGTTTCAGA TTCATCGAGA TGACAGATCC ACAGATCGCC TGCCTTCAGC TCACACATGT 
TTTAATCAAC TGGACCTGCC TGCCTATGAG AGCTTTGAGA AGCTCCGCCA CATGCTACTA 
TTGGCCATCC AGGAATGCTC TGAAGGCTTT GGGCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Huwe1 ( XM_030251457.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107317.1 Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X35, mRNA.

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CloneID	OMu90434
Clone ID Related Accession (Same CDS sequence)	XM_030251457.1
Accession Version	XM_030251457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGATGGAG AACAGGATGG AACTGCTGGA AGCATGGATG CATCTGCCCA GGGTTTATTA 
GAAGGAATTG AACTAGATGG TGACACACTG GCACCCATGG AGACAGATGA ACCTTCTTCT 
TCAGATTCTA AGGGCAAATC TAAAATCACA CCTGCCATGG CTGCAAGAAT TAAACAAATC 
AAGCCGTTGC TGTCAGCTTC TTCCAGATTA GGCCGAGCAC TTGCTGAGCT CTTTGGACTC 
CTTGTTAAGC TTTGTGTGGG TTCTCCTGTC CGCCAAAGGA GGAGCCATCA TGCTGCCAGT 
ACCACCACTG CACCAACACC TGCTGCTAGA TCAACAGCTT CAGCCCTCAC TAAGCTACTC 
ACAAAAGGCT TATCATGGCA GCCCCCACCA TATACACCCA CTCCCCGCTT CAGGCTGACG 
TTCTTCATCT GTTCAGTTGG ATTCACCTCT CCAATGCTCT TTGATGAGAG AAAGTATCCT 
TACCACCTTA TGCTGCAGAA ATTTCTCTGC TCTGGTGGTC ACAATGCTCT TTTTGAAACT 
TTCAACTGGG CTCTATCCAT GGGAGGAAAA GTTCCTGTTT CTGAGGGATT GGAACATTCA 
GATCTACCTG ATGGTACAGG AGAATTCCTA GATGCCTGGC TTATGCTTGT AGAAAAGATG 
GTAAATCCCA CCACGGTGCT TGAATCTCCA CATTCGTTGC CTGCTAAATT GCCTGGAGGA 
GTCCAGAGCT TTCCACAATT TAGTGCCCTT CGCTTCCTTG TAGTAACTCA AAAAGCAGCC 
TTTACTTGTA TCAAGAACCT GTGGAACCGG AAACCCCTTA AAGTATATGG GGGAAGAATG 
GCTGAGTCTA TGCTGGCCAT TTTGTGCCAC ATTCTCCGAG GGGAACCTGT GATTCGAGAA 
AGACTGAGCA AAGAGAAGGA AGGATCTCGA GGAGAAGAAG AAGCAGGCCA GGAAGAAGGT 
GGCTCACGCA GAGAACCTCA AGTCAACCAG CAACAGCTAC AACAGCTCAT GGACATGGGT 
TTCACAAGAG AACATGCCAT GGAGGCACTG TTGAATACCA GTACGATGGA ACAAGCAACA 
GAGTACCTTT TAACCCACCC TCCTCCAATC ATAGGAGGAG TTGTTCGGGA TCTTAGCATG 
TCAGAAGAGG ACCAGATGAT GAGAGCAATT GCTATGTCTC TGGGACAGGA TATTCCAATG 
GATCAAAGGG CAGAGTCACC TGAGGAAGTT GCTTGCCGGA AGGAGGAAGA AGAGCGTAAA 
GCTCGAGAAA AACAAGAAGA GGAAGAAGCT AAATGTCTAG AGAAGTTTCA AGATGCTGAC 
CCCTTGGAAC AAGATGAGCT CCACACTTTC ACAGATACAA TGTTACCAGG CTGCTTCCAT 
CTTCTTGATG AGTTGCCTGA TACAGTGTAC CGTGTTTGTG ATCTGATCAT GACAGCTATC 
AAACGTAATG GAGCTGACTA TCGTGATATG ATTCTAAAGC AAGTTGTCAA CCAGGTGTGG 
GAAGCTGCTG ATGTATTGAT CAAAGCTGCT CTTCCTCTAA CAACAAGTGA CACAAAAACA 
GTTTCAGAGT GGATCAGTCA GATGGCTACC CTGCCACAGG CCTCCAATTT GGCTACTAGA 
ATCTTGCTTT TAACACTACT TTTTGAGGAG TTGAAGTTAC CTTGTGCCTG GGTGGTTGAA 
TCAAGTGGCA TTCTTAATGT CCTAATCAAA CTCTTAGAAG TGGTTCAACC CTGCCTACAG 
GCTGCCAAAG AGCAAAAGGA AGTCCAGACC CCAAAGTGGA TCACCCCAGT GTTGCTCCTT 
ATTGATTTTT ATGAAAAGAC AGCCATCTCA TCAAAGAGAA GAGCCCAAAT GACTAAGTAC 
CTACAGTCCA ACAGCAACAA CTGGCGCTGG TTTGATGACC GCTCTGGGCG TTGGTGTAGT 
TACAGTGCTA GCAACAACAG CACCATTGAT TCTGCCTGGA AATCTGGAGA AACAAGTGTA 
AGATTCACTG CTGGCCGACG AAGATACACT GTCCAGTTCA CTACAATGGT GCAGGTTAAT 
GAAGAAACAG GAAACCGACG CCCTGTGATG CTGACTCTCC TCAGGGTGCC ACGACTGAGT 
AAGAACTCAA AAAGCAGCAA TGGACAGGAA CTAGAGAAGA CACTGGAAGA AAGCAAGGAG 
ACCGATATCA AACATAAAGA AAATAAAGGC AATGATATCC CCTTAGCTCT AGAGAGCACA 
AACACTGAAA AAGAGGCAAG CCTGGATGAA ACAAAAATTG GGGAGATCCT AATCCAGGGC 
CTGACAGAAG ATATGGTGAC TGTTCTAATC CGTGCCTGTG TGAGCATGCT GGGAGTCCCT 
GTGGACCCAG ACACTTTGCA TGCCACCCTT CGTCTCTGTT TAAGGCTCAC CCGAGACCAC 
AAATATGCCA TGATGTTTGC AGAACTGAAG AGTACACGAA TGATCTTGAA TTTGACCCAG 
AGCTCAGGTT TCAATGGGTT TACCCCCTTA GTCACCCTTC TCTTAAGGCA CATCATTGAG 
GATCCTTGTA CTCTTCGTCA TACTATGGAA AAGGTTGTTC GCTCGGCAGC CACAAGTGGA 
GCTGGTAGTA CTACATCTGG TGTTGTATCT GGCAGCCTCG GTTCTCGGGA GATCAACTAT 
ATCCTTAGAG TCCTAGGACC AGCTGCCTGC CGCAATCCAG ACATATTCAC AGAAGTAGCC 
AATTGCTGTA TCCGCATTGC CCTTCCAGCC CCTCGAGGCT CAGGAACTGC TTCAGATGAT 
GAATTTGAGA ATCTTAGAAT TAAAGGTCCT AATGCTGTAC AGCTGGTAAA GACAACACCT 
TTGAAGCCCT CATCTCTGCC TGTCATTCCT GATACTATTA AGGAAGTTAT CTATGATATG 
CTGAATGCTT TGGCTGCATA CCATGCTCCA GAGGAAGCAG ACAAATCTGA TCCTAAACCT 
GGGGGAACGA CCCAAGAGGT TGGCCAGCTC CTGCAAGACA TGGGTGACGA TGTATACCAG 
CAGTACCGGT CACTTACTCG TCAGAGCAGT GACTTTGATA CACAGTCAGG TTTTTCCTTA 
AATAGTCAGG TTTTTGCTGC AGATGGTGCC CCTGCTGAAA CTTCCACAAC TGGAACCTCC 
CAAGGAGAGG GAGCTTCAAC TCCAGAGGAG ACTCGAGAAG GGAAGAAAGA TAAAGAAGGG 
GACCGTACAT CTGAGGAAGG CAAGCAGAAA AGCAAGGGCA GCAAACCTTT AATGCCTACC 
TCTACTATCC TTCGTCTTCT GGCAGAGTTG GTGAGGTCCT ATGTTGGTAT TGCTACCCTG 
ATTGCAAACT ACAGCTATAC CGTGGGCCAG TCTGAGCTGA TCAAAGAGGA CTGCAGTGTG 
CTAGCTTTTG TCCTGGACCA CCTCCTCCCA CACACCCAGA ATGCAGAAGA CAAGGATACA 
CCTGCCCTGG CACGTCTATT CCTTGCAAGT CTGGCTGCTG CGGGGAGTGG CACAGATGCT 
CAAGTGGCCC TAGTGAATGA AGTGAAAGCA GCCCTGGGAC GGGCGCTTGC TATGGCTGAG 
AGTACAGAGA AGCACGCCAG ACTTCAGGCA GTGATGTGTA TCATCAGTAC TATCATGGAA 
TCCTGCCCCT CCACCTCCAG CTTCTACAGT AGTGCTACAG CCAAGACTCA GCATAATGGC 
ATGAACAACA TCATTCGACT GTTCCTAAAG AAGGGACTAG TCAATGACCT GGCCAGAGTT 
CCTCACAGCC TAGACCTGTC CAGTCCCAAT ATGGCCAACA CAGTCAATGC TGCTCTAAAA 
CCCTTGGAAA CACTTTCCCG GATTGTAAAT CAACCCAGTA GCCTTTTTGG CAGCAAGAGT 
GCTTCTAGCA AGAACAAGTC TGAGCAGGAT GCTCAAGGAG CTTCTCAGGA CTCCAGCAGC 
CATCAGCAAG ATCCAGGCGA GCCTGGGGAG GCAGAAGTGC AGGAGGAGGA TCATGATGTC 
ACTCAGACAG AGGTGGCAGA TGGGGATATC ATGGATGGGG AGGCTGAAAC CGACTCAGTG 
GTGATTGCCG GGCAGCCTGA GGTGCTCAGT TCACAAGAGA TGCAGGTTGA AAATGAACTG 
GAGGACCTGA TAGATGAGTT GCTTGAGAGG GATGGCGGAT CTGGGAACAG TACAATTATA 
GTGAGCAGAA GTGGAGAGGA TGAATCACAA GAGGACGTGC TGATGGATGA AGCTCCTTCC 
AACCTCAGCC AAGCTTCCAC CTTGCAGGCC AACCGAGAAG ATTCCATGAA TATCCTGGAC 
CCTGAGGATG AGGAGGAACA TACTCAGGAA GAGGACAGCA GTGGCAGTAA CGAGGATGAG 
GATGATAGTC AGGATGAAGA GGAGGAGGAG GAGGAAGATG AGGAAGATGA TCAGGAGGAT 
GATGAAGGTG AAGAAGGAGA TGAAGATGAT GACGACGATG GTTCTGAGAT GGAATTGGAT 
GAGGATTACC CTGATATGAA CGCTTCTCCC TTGGTCCGAT TTGAGCGCTT TGACCGGGAG 
GATGATCTCA TCATTGAGTT TGACAACATG TTCTCCAGTG CTACAGACAT TCCCCCATCC 
CCAGGAAATA TCCCTACCAC CCACCCATTG ATGGTACGTC ATGCAGATCA CAGTTCCCTG 
ACACTGGGCA GTGGTTCTTC CACAACTCGT CTTACCCAGG GCATCGGTCG CAGTCAAAGG 
ACCCTCAGAC AGCTCACTGC CAATACAGGC CATACCATTC ATGTTCACTA CCCTGGGAAT 
CGCCAGCCTA ACCCTCCTCT TATACTCCAG AGGTTGCTTG GTCCCTCTGC AGCTGCTGAC 
ATTCTTCAGC TGAGCAGCAG CCTTCCATTA CAAAGCCGAG GCCGGGCCCG CCTCCTAGTG 
GGCAATGATG ACGTCCATAT CATCGCCAGG TCTGATGACG AGCTGCTGGA TGACTTTTTT 
CATGACCAGA GTACAGCCAC TAGCCAAGCA GGAACCCTGT CCAGCATCCC AACAGCTCTA 
ACCCGATGGA CAGAGGAATG CAAAGTTCTT GATGCTGAGA GTATGCATGA CTGTGTTTCA 
GTGGTTAAAG TGCCCATTGT CAATCACCTG GAATTCTTGA GAGATGAGGA GCTAGAAGAG 
AGAAGGGAGA AACGCAGGAA ACAATTGGCT GAGGAAGAAA CAAAAATAAT AGACAAAGGC 
AAAGAAGACA AGGAGAACAG AGATCAGAGT GCACAGTGTA CTGTAACTAA GACAAATGAC 
TCCACTGAAC AGAATGTCTC AGATGGGACT CCTATGCCTG ACAGCTATCC AACAACCCCC 
TCTTCAACGG ATGCACCTAC ATCTGAGTCC AAGGAAACAC TTGGTACCCT ACAACCCTCC 
CAGCAACAGC CAGCACTTCC ACCCCCACCA TCCTTAGGAG AGATTCCTCA GGAACTACAG 
TCCCCTGCTG AAGAAGTGGC AAACTCTACA CAGCTATTGA TGCCTATAGA GTTGGAGGAA 
TTGGGTCCCA CAAGACCAAG TGGAGAAGCA GAAACAACTC AAATGGAATT GTCTCCAGCT 
CCCACCATAA CCTCTCTTTC CCCAGAGAGA GCTGAAGATT CAGACGCGTT AACAGCTGTC 
AGCAGTCAGC TGGAAGGTTC TCCCATGGAC ACCAGCAGCC TGGCTTCCTG TACGTTAGAA 
GAGGCTGTGG GTGACACTCC AGCAGCTGGC AGTTCTGAGC AGCCCACAGC AGGTAGTTCC 
ACTCCTGGGG ATGCGCCATC AGTTGTGGCA GAAGTGCAAG GCAGACCTGA TGTATCAAGA 
GAATCCAACC AGCCACCTGA GGACAGCTCC CCCCCTGCAT CCTCGGAGAG TTCTTCCACT 
AGAGATTCTG CCGTGGCCAT CTCTGGAGCA GATTCTCGTG GAATTCTAGA AGAGCCACTA 
CCTTCAACAA GCAGTGAAGA AGAAGATCCC CTTGCTGGTA TCAGTCTCCC TGAAGGTGTG 
GATCCCTCCT TTCTGGCTGC TCTCCCTGAT GATATCCGTC GGGAAGTCCT ACAGAACCAG 
CTGGGCATAC GTCCACCAAC CCGAAGTGCT CCCTCCTCAA ATAGCTCAGC TCCTGCAGTA 
GTGGGGAACC CTGGTGTAAC TGAAGTGAGC CCTGAGTTCC TGGCTGCCCT ACCTCCAGCT 
ATTCAAGAGG AAGTGCTAGC ACAGCAGAGA GCTGAACAGC AGCGACGGGA ACTGGCTCAG 
AATGCGAGCT CAGACACCCC CATGGACCCT GTGACTTTCA TTCAGACTTT GCCCTCAGAT 
CTGCGCCGTA GTGTCCTAGA GGATATGGAA GACAGTGTGT TAGCTGTGAT GCCACCTGAC 
ATTGCAGCTG AGGCTCAAGC TCTAAGACGA GAACAAGAAG CCCGACAGCG GCAGCTCATG 
CATGAGCGTC TTTTTGGACA CAGTAGTACT TCTGCACTCT CTGCCATTCT CCGAAGCCCG 
GCTTTTACCA GTCGCCTGAG TGGCAACCGT GGGGTCCAGT ATACTCGCCT TGCTGTACAG 
AGGGGTGGCA CCTTCCAGAT GGGGGGTAGC AGCAGCCATA ATAGGCCTTC TGGCAGTAAT 
GTGGACACAC TACTCCGCCT CCGAGGACGA CTCCTTCTAG ACCATGAAGC CCTGTCTTGC 
CTCTTGGTCC TACTTTTTGT GGATGAGCCA AAGCTCAATA CTAGCCGTCT ACACCGAGTA 
CTGAGAAATC TCTGCTATCA TGCCCAGACC CGCCACTGGG TCATCCGCAG CCTCCTGTCC 
ATTTTGCAGC GCAGCAGTGA AAGTGAGTTA TGCATTGAGA CACCTAAGCT CTCCACAAGT 
GAAGAAAGGG GCAAGAAATC AAGCAAGAGC TGTGCATCAA GCAGTCATGA GAACCGTCCC 
CTGGATCTGC TCCACAAGAT GGAGTCCAAA AGTTCCAATC AGCTCTCCTG GCTCTCAGTA 
TCAATGGATG CTGCTTTAGG CTGTAGGACT AACATATTCC AGATTCAACG TTCTGGAGGG 
CGTAAACATA CAGAGAAGCA TGCAAGCAGT GGTTCCACTG TCCACATCCA CCCCCAGGCT 
GCTCCTGTTG TTTGCAGACA TGTTTTGGAT ACACTCATTC AGTTGGCCAA GGTGTTTCCC 
AGCCACTTTA CACAGCAGCG GACCAAAGAA ACAAACTGTG AGAGTGATCG GGAACGGGGC 
AGTAAGCAGG CCTGCAGCCC ATGCTCCTCA CAGTCCTCCA GCAGTGGCAT CTGTACAGAC 
TTCTGGGACT TATTGGTAAA ACTGGACAAC ATGAATGTTA GCCGAAAAGG CAAGAACTCC 
GTGAAGTCAG TACCAGTGAG CTCTGGTGGT GAAGGGGAAA CTTCCCCACA CAGCCTTGAA 
GCCTCTCCAC TGGGGCAGCT CATGAACATG TTGTCACATC CAGTCATCCG CCGGAGTTCT 
CTCTTAACTG AGAAACTCCT GAGACTCCTC TCTCTCATCT CAATTGCTCT CCCAGAAAAC 
AAAGTATCAG AAGTGCAAAC TAACTCCAGC AACAGTGGTT CCTCCACTGC TGCCACCTCA 
AACACATCTA CTACTACCAC CACCACCACT ACTGCCACTG CCCCCACGCC CACACCCCCA 
GCTGCAACTA CCCCTGTCAC TTCTGCTCCA GCTCTGGTTG CTGCCACAGC TATTTCCACC 
ATTACTGTAG CTGCTTCTAC CACAGTGACT ACCCCCACAA CTGCTACCAC TACTGTTTCA 
ACTTCTACTA CTAAAGGCAG CAAATCTCCA GCAAAGGTTG GTGAAGGAGG CAGTGGTATA 
GACTTCAAAA TGGTGTCCTC TGGCCTCACT GAAAACCAGC TACAGCTCTC TGTAGAGGTG 
TTGACATCCC ACTCTTGTTC TGAAGAAGGC TTGGAAGATG CGGCCAATGT GTTACTGCAG 
CTCTCTCGTG GGGACTCTGG TACCCGGGAC ACTGTTCTTA AGCTGCTACT AAATGGAGCC 
CGCCATCTGG GTTATACCCT CTGTAAACAG ATAGGTACCC TACTCGCTGA ACTACGGGAA 
TATAATCTGG AGCAGCAGCG GCGAGCCCAA TGCGAGACCC TCTCTCCTGA TGGCCTACCT 
GAGGAGCAAC CACAGACCAC CAAACTGAAA GGCAAAATGC AGAGCAGGTT TGACATGGCT 
GAAAATGTGG TAATTGTGGC ATCTCAGAAG CGACCCTTGG GTGGCCGGGA GCTCCAGCTG 
CCTTCTATGT CCATGTTGAC ATCCAAGACA TCTACCCAGA AGTTCTTCTT GAGGGTACTA 
CAGGTCATCA TCCAGCTGCG GGATGACACA CGACGAGCTA ACAAGAAAGC CAAGCAGACA 
GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG 
CTGGAGGCTG AGGCTGATGC CATTATACAA ATGGTACGTG AGGGTCAAAG GGCGCGGAGA 
CAGCAACAAG CAGCAACGTC GGAGTCTTCT AACCAGTCAG AGACATCTGT CCGGAGAGAG 
GAATCGCCCA TGGATGTGGA CCAGCCATCT CCTAGTGCTC AAGATACTCA GTCCATTGTA 
ATCTCAGATG GAACACCACA GGGAGAGAAG GAGAAAGAGG AGAAACCACC TGAGTTACCC 
CTGCTCAGTG AGCAACTGAG CCTAGATGAA CTGTGGGACA TGCTAGGGGA ATGTTTAAAA 
GAACTGGAGG AATCTCATGA CCAGCATGCA GTATTAGTGC TACAGCCTGC TGTCGAGGCC 
TTCTTTTTGG TCCATGCCAC AGAGCGGGAG AGCAAGCCTC CTGTCCGAGA TACCCGTGAG 
AGCCAGCTGG CACACATCAA GGATGAACCT CCTCCACTCT CCCCCGCCCC CTTAACCCCA 
GCCACTCCAT CCTCCCTTGA CCCATTCTTC TCCAGGGAGC CCTCATCTAT GCACATTTCC 
TCGAGCCTGC CCCCTGACAC ACAGAAGTTC CTTCGCTTTG CAGAGACTCA CCGCACTGTG 
TTAAACCAGA TACTACGGCA GTCTACCACC CATCTTGCTG ATGGGCCTTT TGCTGTCTTG 
GTAGATTATA TCCGTGTCCT CGACTTTGAT GTCAAGCGCA AATATTTCCG CCAAGAGCTG 
GAGCGTTTGG ACGAAGGGCT CCGGAAAGAA GATATGGCAG TGCATGTCCG CCGTGACCAT 
GTATTTGAAG ACTCCTATCG TGAGCTGCAT CGCAAATCTC CTGAAGAAAT GAAGAATCGA 
TTGTATATAG TGTTTGAAGG AGAAGAAGGG CAGGATGCTG GGGGGCTCCT GCGAGAGTGG 
TACATGATCA TCTCTCGAGA GATGTTCAAC CCTATGTATG CCTTGTTCCG TACCTCACCT 
GGTGACCGGG TCACCTACAC CATCAATCCA TCTTCCCACT GTAACCCCAA CCACCTCAGC 
TACTTCAAGT TTGTTGGACG AATTGTAGCC AAAGCTGTAT ATGACAACCG CCTCCTGGAG 
TGCTACTTTA CTAGGTCTTT CTACAAACAC ATCTTGGGCA AATCTGTCAG GTATACAGAT 
ATGGAGAGTG AAGATTACCA CTTCTACCAG GGCCTAGTTT ATCTGCTTGA AAATGATGTC 
TCTACATTAG GCTATGACCT CACCTTCAGC ACTGAGGTTC AAGAATTTGG AGTATGTGAA 
GTTCGTGACC TCAAACCCAA TGGAGCCAAC ATCTTGGTAA CAGAGGAGAA CAAGAAGGAA 
TATGTACACC TGGTTTGCCA GATGAGAATG ACAGGAGCCA TCCGCAAACA GCTGGCAGCC 
TTCTTGGAGG GCTTCTATGA AATCATTCCA AAGCGCCTCA TATCCATCTT TACTGAGCAG 
GAATTAGAGC TGCTCATATC AGGGCTGCCT ACCATCGACA TTGATGATCT AAAATCTAAC 
ACTGAGTACC ACAAGTACCA ATCCAACTCT ATTCAGATCC AGTGGTTTTG GAGAGCATTG 
CGTTCCTTTG ACCAAGCAGA CCGTGCCAAG TTCCTCCAGT TTGTCACAGG TACTTCCAAG 
GTACCCCTAC AAGGATTTGC TGCCCTTGAA GGTATGAATG GCATTCAGAA GTTTCAGATT 
CATCGAGATG ACAGATCCAC AGATCGCCTG CCTTCAGCTC ACACATGTTT TAATCAACTG 
GACCTGCCTG CCTATGAGAG CTTTGAGAAG CTCCGCCACA TGCTACTATT GGCCATCCAG 
GAATGCTCTG AAGGCTTTGG GCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107317.1
CDS	1856..14965
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKADGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_030251457.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, UBA and WWE domain containing 1 (Huwe1), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251457.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AAGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCTTGGA ACTGCAGCAA 
ATCAAAACAT GGAACATTGG CAAGTGTGAG TTATATCACT GGGTGGATCT GTTGGACCGC 
TTTGATGGAA TTCTGGCAGA TGCTGGACAA ACAGTGGAGA ATATGTCATG GATGCTTGTA 
TGTGATAGGC CAGAAAAAGA ACAGCTGAAA ATGCTTCTAT TGGCTGTATT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCTCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAACCT TCTATATGTA 
TTTAGCAAAA GATCAAACTA TATCACACGT CTTGGATCTG ACAAGAGGAC TCCGCTACTG 
ACACGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA AATGTTGAAA TATCCACCCA GTGCAACCAC ACTACACTTT 
GAATTTTATG CAGATCCTGG AGCTGAGGTC AAAATTGAGA AGAGGACAAC TAGTAATACA 
CTACATTATA TTCACATAGA ACAACTTGAC AAGATTTCAG AAAGTCCTTC TGAAATAATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAT 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGGT TACAAGCTGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTCTATTCT AATGCCTTGC AAGAGTCAGC AAACAGTATC 
TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AAATAACAGA TAAGCAGCTC 
ATGGAAATTA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTTCATTT GGAGAGAACT 
CCCAAGCTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT 
GTACTTGTGC GGAACTGCAT TCAGGCCATG ATTGATCCTT CCATGGACCC ATACCCCCAT 
CAGTTTGCCA CAGCTCTTTT CTCCTTTTTG TACCATCTGG CTAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTACTAA AGGTCATAAA GTTTCTTGGT 
GATGAACAAG ACCAGATAAC ATTTGTAACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGACA TGGCAGCTTT TCAGTCTCAT AGTGGTCTTT CTATCTTCAT CTATAGACTT 
GAGCACGAAG TAGATTTGTG TCGTAAAGAA TGCCCTTTCG TGATCAAGCC AAAGATCCAG 
AGACCCAGTA CCACTCAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTTCAGTGT 
ATTCCACAAC GAGCAGCACT TCTCAAGTCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTTTACC TACCTCTCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGTCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAGCCA TCACTGCTGT CCTCACTCCA GGACAATGGA 
CTGACAGATG TCATGCTGCA TGCACTGCTC ATCAAAGATG TTCCTGCTAC AAGGGAAGTC 
CTCGGTTCCC TCCCAAATGT ATTCAGTGCA CTTTGCTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTACAAT GTCAGCCATT TGAACGTCTC TTCAAAGTTC TTCTGTCTCC TGATTACCTC 
CCAGCCATGC GGAGGAGGAG GAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTTG ATGAGCTCAT GAGACACCAG CCTACCCTCA AAACAGATGC AACAACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ATCCTAAATA CATTTGTCAG 
AAGCCATCTA TTCAGAAAGC GGATGGTACT GCTACTGCTC CTCCTCCAAG GTCTAATCAT 
GCTGCAGAAG AGGCTTCCAG TGAAGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTG CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTCGTTGG TACCGAGGAA 
CGTATACCTA TACCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
TTGAGCAACA ATACAACAGA TGACCACTGT CAAGAATTTG TAAACCAGAA AGGACTCTTA 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACCC TGTCACATGA ACCCAAAGTT 
CTTCAAGAAG GTCTCCTTCA ATTAGACTTG ATCCTTTCCT CCTTGGAGCC TTTACATCGG 
CCGATTGAAT CCCCTGGGGG CTCAGTGTTA CTGAGAGAAT TGGCATGTGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC TCAGGCCACG CCTCTGTTGC ATGCACTCAC TGCAGCCCAT 
GCTTACATCA TGATGTTTGT TCACACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCTATC 
TCAGTAAACC AGTGGGGTTC ACAATTGGGT TTGAGTGTTC TGAGCAAGCT AAGCCAGTTA 
TACTGTTCTC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TATGTACCCC CAACAGCCTA 
CCATCTGGAT GTGAATTTGG TCAAGCGGAC ATGCAGAAAC TAGTTCCAAA GGATGAGAAG 
GCAGATGGAG AACAGGATGG AACTGCTGGA AGCATGGATG CATCTGCCCA GGGTTTATTA 
GAAGGAATTG AACTAGATGG TGACACACTG GCACCCATGG AGACAGATGA ACCTTCTTCT 
TCAGATTCTA AGGGCAAATC TAAAATCACA CCTGCCATGG CTGCAAGAAT TAAACAAATC 
AAGCCGTTGC TGTCAGCTTC TTCCAGATTA GGCCGAGCAC TTGCTGAGCT CTTTGGACTC 
CTTGTTAAGC TTTGTGTGGG TTCTCCTGTC CGCCAAAGGA GGAGCCATCA TGCTGCCAGT 
ACCACCACTG CACCAACACC TGCTGCTAGA TCAACAGCTT CAGCCCTCAC TAAGCTACTC 
ACAAAAGGCT TATCATGGCA GCCCCCACCA TATACACCCA CTCCCCGCTT CAGGCTGACG 
TTCTTCATCT GTTCAGTTGG ATTCACCTCT CCAATGCTCT TTGATGAGAG AAAGTATCCT 
TACCACCTTA TGCTGCAGAA ATTTCTCTGC TCTGGTGGTC ACAATGCTCT TTTTGAAACT 
TTCAACTGGG CTCTATCCAT GGGAGGAAAA GTTCCTGTTT CTGAGGGATT GGAACATTCA 
GATCTACCTG ATGGTACAGG AGAATTCCTA GATGCCTGGC TTATGCTTGT AGAAAAGATG 
GTAAATCCCA CCACGGTGCT TGAATCTCCA CATTCGTTGC CTGCTAAATT GCCTGGAGGA 
GTCCAGAGCT TTCCACAATT TAGTGCCCTT CGCTTCCTTG TAGTAACTCA AAAAGCAGCC 
TTTACTTGTA TCAAGAACCT GTGGAACCGG AAACCCCTTA AAGTATATGG GGGAAGAATG 
GCTGAGTCTA TGCTGGCCAT TTTGTGCCAC ATTCTCCGAG GGGAACCTGT GATTCGAGAA 
AGACTGAGCA AAGAGAAGGA AGGATCTCGA GGAGAAGAAG AAGCAGGCCA GGAAGAAGGT 
GGCTCACGCA GAGAACCTCA AGTCAACCAG CAACAGCTAC AACAGCTCAT GGACATGGGT 
TTCACAAGAG AACATGCCAT GGAGGCACTG TTGAATACCA GTACGATGGA ACAAGCAACA 
GAGTACCTTT TAACCCACCC TCCTCCAATC ATAGGAGGAG TTGTTCGGGA TCTTAGCATG 
TCAGAAGAGG ACCAGATGAT GAGAGCAATT GCTATGTCTC TGGGACAGGA TATTCCAATG 
GATCAAAGGG CAGAGTCACC TGAGGAAGTT GCTTGCCGGA AGGAGGAAGA AGAGCGTAAA 
GCTCGAGAAA AACAAGAAGA GGAAGAAGCT AAATGTCTAG AGAAGTTTCA AGATGCTGAC 
CCCTTGGAAC AAGATGAGCT CCACACTTTC ACAGATACAA TGTTACCAGG CTGCTTCCAT 
CTTCTTGATG AGTTGCCTGA TACAGTGTAC CGTGTTTGTG ATCTGATCAT GACAGCTATC 
AAACGTAATG GAGCTGACTA TCGTGATATG ATTCTAAAGC AAGTTGTCAA CCAGGTGTGG 
GAAGCTGCTG ATGTATTGAT CAAAGCTGCT CTTCCTCTAA CAACAAGTGA CACAAAAACA 
GTTTCAGAGT GGATCAGTCA GATGGCTACC CTGCCACAGG CCTCCAATTT GGCTACTAGA 
ATCTTGCTTT TAACACTACT TTTTGAGGAG TTGAAGTTAC CTTGTGCCTG GGTGGTTGAA 
TCAAGTGGCA TTCTTAATGT CCTAATCAAA CTCTTAGAAG TGGTTCAACC CTGCCTACAG 
GCTGCCAAAG AGCAAAAGGA AGTCCAGACC CCAAAGTGGA TCACCCCAGT GTTGCTCCTT 
ATTGATTTTT ATGAAAAGAC AGCCATCTCA TCAAAGAGAA GAGCCCAAAT GACTAAGTAC 
CTACAGTCCA ACAGCAACAA CTGGCGCTGG TTTGATGACC GCTCTGGGCG TTGGTGTAGT 
TACAGTGCTA GCAACAACAG CACCATTGAT TCTGCCTGGA AATCTGGAGA AACAAGTGTA 
AGATTCACTG CTGGCCGACG AAGATACACT GTCCAGTTCA CTACAATGGT GCAGGTTAAT 
GAAGAAACAG GAAACCGACG CCCTGTGATG CTGACTCTCC TCAGGGTGCC ACGACTGAGT 
AAGAACTCAA AAAGCAGCAA TGGACAGGAA CTAGAGAAGA CACTGGAAGA AAGCAAGGAG 
ACCGATATCA AACATAAAGA AAATAAAGGC AATGATATCC CCTTAGCTCT AGAGAGCACA 
AACACTGAAA AAGAGGCAAG CCTGGATGAA ACAAAAATTG GGGAGATCCT AATCCAGGGC 
CTGACAGAAG ATATGGTGAC TGTTCTAATC CGTGCCTGTG TGAGCATGCT GGGAGTCCCT 
GTGGACCCAG ACACTTTGCA TGCCACCCTT CGTCTCTGTT TAAGGCTCAC CCGAGACCAC 
AAATATGCCA TGATGTTTGC AGAACTGAAG AGTACACGAA TGATCTTGAA TTTGACCCAG 
AGCTCAGGTT TCAATGGGTT TACCCCCTTA GTCACCCTTC TCTTAAGGCA CATCATTGAG 
GATCCTTGTA CTCTTCGTCA TACTATGGAA AAGGTTGTTC GCTCGGCAGC CACAAGTGGA 
GCTGGTAGTA CTACATCTGG TGTTGTATCT GGCAGCCTCG GTTCTCGGGA GATCAACTAT 
ATCCTTAGAG TCCTAGGACC AGCTGCCTGC CGCAATCCAG ACATATTCAC AGAAGTAGCC 
AATTGCTGTA TCCGCATTGC CCTTCCAGCC CCTCGAGGCT CAGGAACTGC TTCAGATGAT 
GAATTTGAGA ATCTTAGAAT TAAAGGTCCT AATGCTGTAC AGCTGGTAAA GACAACACCT 
TTGAAGCCCT CATCTCTGCC TGTCATTCCT GATACTATTA AGGAAGTTAT CTATGATATG 
CTGAATGCTT TGGCTGCATA CCATGCTCCA GAGGAAGCAG ACAAATCTGA TCCTAAACCT 
GGGGGAACGA CCCAAGAGGT TGGCCAGCTC CTGCAAGACA TGGGTGACGA TGTATACCAG 
CAGTACCGGT CACTTACTCG TCAGAGCAGT GACTTTGATA CACAGTCAGG TTTTTCCTTA 
AATAGTCAGG TTTTTGCTGC AGATGGTGCC CCTGCTGAAA CTTCCACAAC TGGAACCTCC 
CAAGGAGAGG GAGCTTCAAC TCCAGAGGAG ACTCGAGAAG GGAAGAAAGA TAAAGAAGGG 
GACCGTACAT CTGAGGAAGG CAAGCAGAAA AGCAAGGGCA GCAAACCTTT AATGCCTACC 
TCTACTATCC TTCGTCTTCT GGCAGAGTTG GTGAGGTCCT ATGTTGGTAT TGCTACCCTG 
ATTGCAAACT ACAGCTATAC CGTGGGCCAG TCTGAGCTGA TCAAAGAGGA CTGCAGTGTG 
CTAGCTTTTG TCCTGGACCA CCTCCTCCCA CACACCCAGA ATGCAGAAGA CAAGGATACA 
CCTGCCCTGG CACGTCTATT CCTTGCAAGT CTGGCTGCTG CGGGGAGTGG CACAGATGCT 
CAAGTGGCCC TAGTGAATGA AGTGAAAGCA GCCCTGGGAC GGGCGCTTGC TATGGCTGAG 
AGTACAGAGA AGCACGCCAG ACTTCAGGCA GTGATGTGTA TCATCAGTAC TATCATGGAA 
TCCTGCCCCT CCACCTCCAG CTTCTACAGT AGTGCTACAG CCAAGACTCA GCATAATGGC 
ATGAACAACA TCATTCGACT GTTCCTAAAG AAGGGACTAG TCAATGACCT GGCCAGAGTT 
CCTCACAGCC TAGACCTGTC CAGTCCCAAT ATGGCCAACA CAGTCAATGC TGCTCTAAAA 
CCCTTGGAAA CACTTTCCCG GATTGTAAAT CAACCCAGTA GCCTTTTTGG CAGCAAGAGT 
GCTTCTAGCA AGAACAAGTC TGAGCAGGAT GCTCAAGGAG CTTCTCAGGA CTCCAGCAGC 
CATCAGCAAG ATCCAGGCGA GCCTGGGGAG GCAGAAGTGC AGGAGGAGGA TCATGATGTC 
ACTCAGACAG AGGTGGCAGA TGGGGATATC ATGGATGGGG AGGCTGAAAC CGACTCAGTG 
GTGATTGCCG GGCAGCCTGA GGTGCTCAGT TCACAAGAGA TGCAGGTTGA AAATGAACTG 
GAGGACCTGA TAGATGAGTT GCTTGAGAGG GATGGCGGAT CTGGGAACAG TACAATTATA 
GTGAGCAGAA GTGGAGAGGA TGAATCACAA GAGGACGTGC TGATGGATGA AGCTCCTTCC 
AACCTCAGCC AAGCTTCCAC CTTGCAGGCC AACCGAGAAG ATTCCATGAA TATCCTGGAC 
CCTGAGGATG AGGAGGAACA TACTCAGGAA GAGGACAGCA GTGGCAGTAA CGAGGATGAG 
GATGATAGTC AGGATGAAGA GGAGGAGGAG GAGGAAGATG AGGAAGATGA TCAGGAGGAT 
GATGAAGGTG AAGAAGGAGA TGAAGATGAT GACGACGATG GTTCTGAGAT GGAATTGGAT 
GAGGATTACC CTGATATGAA CGCTTCTCCC TTGGTCCGAT TTGAGCGCTT TGACCGGGAG 
GATGATCTCA TCATTGAGTT TGACAACATG TTCTCCAGTG CTACAGACAT TCCCCCATCC 
CCAGGAAATA TCCCTACCAC CCACCCATTG ATGGTACGTC ATGCAGATCA CAGTTCCCTG 
ACACTGGGCA GTGGTTCTTC CACAACTCGT CTTACCCAGG GCATCGGTCG CAGTCAAAGG 
ACCCTCAGAC AGCTCACTGC CAATACAGGC CATACCATTC ATGTTCACTA CCCTGGGAAT 
CGCCAGCCTA ACCCTCCTCT TATACTCCAG AGGTTGCTTG GTCCCTCTGC AGCTGCTGAC 
ATTCTTCAGC TGAGCAGCAG CCTTCCATTA CAAAGCCGAG GCCGGGCCCG CCTCCTAGTG 
GGCAATGATG ACGTCCATAT CATCGCCAGG TCTGATGACG AGCTGCTGGA TGACTTTTTT 
CATGACCAGA GTACAGCCAC TAGCCAAGCA GGAACCCTGT CCAGCATCCC AACAGCTCTA 
ACCCGATGGA CAGAGGAATG CAAAGTTCTT GATGCTGAGA GTATGCATGA CTGTGTTTCA 
GTGGTTAAAG TGCCCATTGT CAATCACCTG GAATTCTTGA GAGATGAGGA GCTAGAAGAG 
AGAAGGGAGA AACGCAGGAA ACAATTGGCT GAGGAAGAAA CAAAAATAAT AGACAAAGGC 
AAAGAAGACA AGGAGAACAG AGATCAGAGT GCACAGTGTA CTGTAACTAA GACAAATGAC 
TCCACTGAAC AGAATGTCTC AGATGGGACT CCTATGCCTG ACAGCTATCC AACAACCCCC 
TCTTCAACGG ATGCACCTAC ATCTGAGTCC AAGGAAACAC TTGGTACCCT ACAACCCTCC 
CAGCAACAGC CAGCACTTCC ACCCCCACCA TCCTTAGGAG AGATTCCTCA GGAACTACAG 
TCCCCTGCTG AAGAAGTGGC AAACTCTACA CAGCTATTGA TGCCTATAGA GTTGGAGGAA 
TTGGGTCCCA CAAGACCAAG TGGAGAAGCA GAAACAACTC AAATGGAATT GTCTCCAGCT 
CCCACCATAA CCTCTCTTTC CCCAGAGAGA GCTGAAGATT CAGACGCGTT AACAGCTGTC 
AGCAGTCAGC TGGAAGGTTC TCCCATGGAC ACCAGCAGCC TGGCTTCCTG TACGTTAGAA 
GAGGCTGTGG GTGACACTCC AGCAGCTGGC AGTTCTGAGC AGCCCACAGC AGGTAGTTCC 
ACTCCTGGGG ATGCGCCATC AGTTGTGGCA GAAGTGCAAG GCAGACCTGA TGTATCAAGA 
GAATCCAACC AGCCACCTGA GGACAGCTCC CCCCCTGCAT CCTCGGAGAG TTCTTCCACT 
AGAGATTCTG CCGTGGCCAT CTCTGGAGCA GATTCTCGTG GAATTCTAGA AGAGCCACTA 
CCTTCAACAA GCAGTGAAGA AGAAGATCCC CTTGCTGGTA TCAGTCTCCC TGAAGGTGTG 
GATCCCTCCT TTCTGGCTGC TCTCCCTGAT GATATCCGTC GGGAAGTCCT ACAGAACCAG 
CTGGGCATAC GTCCACCAAC CCGAAGTGCT CCCTCCTCAA ATAGCTCAGC TCCTGCAGTA 
GTGGGGAACC CTGGTGTAAC TGAAGTGAGC CCTGAGTTCC TGGCTGCCCT ACCTCCAGCT 
ATTCAAGAGG AAGTGCTAGC ACAGCAGAGA GCTGAACAGC AGCGACGGGA ACTGGCTCAG 
AATGCGAGCT CAGACACCCC CATGGACCCT GTGACTTTCA TTCAGACTTT GCCCTCAGAT 
CTGCGCCGTA GTGTCCTAGA GGATATGGAA GACAGTGTGT TAGCTGTGAT GCCACCTGAC 
ATTGCAGCTG AGGCTCAAGC TCTAAGACGA GAACAAGAAG CCCGACAGCG GCAGCTCATG 
CATGAGCGTC TTTTTGGACA CAGTAGTACT TCTGCACTCT CTGCCATTCT CCGAAGCCCG 
GCTTTTACCA GTCGCCTGAG TGGCAACCGT GGGGTCCAGT ATACTCGCCT TGCTGTACAG 
AGGGGTGGCA CCTTCCAGAT GGGGGGTAGC AGCAGCCATA ATAGGCCTTC TGGCAGTAAT 
GTGGACACAC TACTCCGCCT CCGAGGACGA CTCCTTCTAG ACCATGAAGC CCTGTCTTGC 
CTCTTGGTCC TACTTTTTGT GGATGAGCCA AAGCTCAATA CTAGCCGTCT ACACCGAGTA 
CTGAGAAATC TCTGCTATCA TGCCCAGACC CGCCACTGGG TCATCCGCAG CCTCCTGTCC 
ATTTTGCAGC GCAGCAGTGA AAGTGAGTTA TGCATTGAGA CACCTAAGCT CTCCACAAGT 
GAAGAAAGGG GCAAGAAATC AAGCAAGAGC TGTGCATCAA GCAGTCATGA GAACCGTCCC 
CTGGATCTGC TCCACAAGAT GGAGTCCAAA AGTTCCAATC AGCTCTCCTG GCTCTCAGTA 
TCAATGGATG CTGCTTTAGG CTGTAGGACT AACATATTCC AGATTCAACG TTCTGGAGGG 
CGTAAACATA CAGAGAAGCA TGCAAGCAGT GGTTCCACTG TCCACATCCA CCCCCAGGCT 
GCTCCTGTTG TTTGCAGACA TGTTTTGGAT ACACTCATTC AGTTGGCCAA GGTGTTTCCC 
AGCCACTTTA CACAGCAGCG GACCAAAGAA ACAAACTGTG AGAGTGATCG GGAACGGGGC 
AGTAAGCAGG CCTGCAGCCC ATGCTCCTCA CAGTCCTCCA GCAGTGGCAT CTGTACAGAC 
TTCTGGGACT TATTGGTAAA ACTGGACAAC ATGAATGTTA GCCGAAAAGG CAAGAACTCC 
GTGAAGTCAG TACCAGTGAG CTCTGGTGGT GAAGGGGAAA CTTCCCCACA CAGCCTTGAA 
GCCTCTCCAC TGGGGCAGCT CATGAACATG TTGTCACATC CAGTCATCCG CCGGAGTTCT 
CTCTTAACTG AGAAACTCCT GAGACTCCTC TCTCTCATCT CAATTGCTCT CCCAGAAAAC 
AAAGTATCAG AAGTGCAAAC TAACTCCAGC AACAGTGGTT CCTCCACTGC TGCCACCTCA 
AACACATCTA CTACTACCAC CACCACCACT ACTGCCACTG CCCCCACGCC CACACCCCCA 
GCTGCAACTA CCCCTGTCAC TTCTGCTCCA GCTCTGGTTG CTGCCACAGC TATTTCCACC 
ATTACTGTAG CTGCTTCTAC CACAGTGACT ACCCCCACAA CTGCTACCAC TACTGTTTCA 
ACTTCTACTA CTAAAGGCAG CAAATCTCCA GCAAAGGTTG GTGAAGGAGG CAGTGGTATA 
GACTTCAAAA TGGTGTCCTC TGGCCTCACT GAAAACCAGC TACAGCTCTC TGTAGAGGTG 
TTGACATCCC ACTCTTGTTC TGAAGAAGGC TTGGAAGATG CGGCCAATGT GTTACTGCAG 
CTCTCTCGTG GGGACTCTGG TACCCGGGAC ACTGTTCTTA AGCTGCTACT AAATGGAGCC 
CGCCATCTGG GTTATACCCT CTGTAAACAG ATAGGTACCC TACTCGCTGA ACTACGGGAA 
TATAATCTGG AGCAGCAGCG GCGAGCCCAA TGCGAGACCC TCTCTCCTGA TGGCCTACCT 
GAGGAGCAAC CACAGACCAC CAAACTGAAA GGCAAAATGC AGAGCAGGTT TGACATGGCT 
GAAAATGTGG TAATTGTGGC ATCTCAGAAG CGACCCTTGG GTGGCCGGGA GCTCCAGCTG 
CCTTCTATGT CCATGTTGAC ATCCAAGACA TCTACCCAGA AGTTCTTCTT GAGGGTACTA 
CAGGTCATCA TCCAGCTGCG GGATGACACA CGACGAGCTA ACAAGAAAGC CAAGCAGACA 
GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG 
CTGGAGGCTG AGGCTGATGC CATTATACAA ATGGTACGTG AGGGTCAAAG GGCGCGGAGA 
CAGCAACAAG CAGCAACGTC GGAGTCTTCT AACCAGTCAG AGACATCTGT CCGGAGAGAG 
GAATCGCCCA TGGATGTGGA CCAGCCATCT CCTAGTGCTC AAGATACTCA GTCCATTGTA 
ATCTCAGATG GAACACCACA GGGAGAGAAG GAGAAAGAGG AGAAACCACC TGAGTTACCC 
CTGCTCAGTG AGCAACTGAG CCTAGATGAA CTGTGGGACA TGCTAGGGGA ATGTTTAAAA 
GAACTGGAGG AATCTCATGA CCAGCATGCA GTATTAGTGC TACAGCCTGC TGTCGAGGCC 
TTCTTTTTGG TCCATGCCAC AGAGCGGGAG AGCAAGCCTC CTGTCCGAGA TACCCGTGAG 
AGCCAGCTGG CACACATCAA GGATGAACCT CCTCCACTCT CCCCCGCCCC CTTAACCCCA 
GCCACTCCAT CCTCCCTTGA CCCATTCTTC TCCAGGGAGC CCTCATCTAT GCACATTTCC 
TCGAGCCTGC CCCCTGACAC ACAGAAGTTC CTTCGCTTTG CAGAGACTCA CCGCACTGTG 
TTAAACCAGA TACTACGGCA GTCTACCACC CATCTTGCTG ATGGGCCTTT TGCTGTCTTG 
GTAGATTATA TCCGTGTCCT CGACTTTGAT GTCAAGCGCA AATATTTCCG CCAAGAGCTG 
GAGCGTTTGG ACGAAGGGCT CCGGAAAGAA GATATGGCAG TGCATGTCCG CCGTGACCAT 
GTATTTGAAG ACTCCTATCG TGAGCTGCAT CGCAAATCTC CTGAAGAAAT GAAGAATCGA 
TTGTATATAG TGTTTGAAGG AGAAGAAGGG CAGGATGCTG GGGGGCTCCT GCGAGAGTGG 
TACATGATCA TCTCTCGAGA GATGTTCAAC CCTATGTATG CCTTGTTCCG TACCTCACCT 
GGTGACCGGG TCACCTACAC CATCAATCCA TCTTCCCACT GTAACCCCAA CCACCTCAGC 
TACTTCAAGT TTGTTGGACG AATTGTAGCC AAAGCTGTAT ATGACAACCG CCTCCTGGAG 
TGCTACTTTA CTAGGTCTTT CTACAAACAC ATCTTGGGCA AATCTGTCAG GTATACAGAT 
ATGGAGAGTG AAGATTACCA CTTCTACCAG GGCCTAGTTT ATCTGCTTGA AAATGATGTC 
TCTACATTAG GCTATGACCT CACCTTCAGC ACTGAGGTTC AAGAATTTGG AGTATGTGAA 
GTTCGTGACC TCAAACCCAA TGGAGCCAAC ATCTTGGTAA CAGAGGAGAA CAAGAAGGAA 
TATGTACACC TGGTTTGCCA GATGAGAATG ACAGGAGCCA TCCGCAAACA GCTGGCAGCC 
TTCTTGGAGG GCTTCTATGA AATCATTCCA AAGCGCCTCA TATCCATCTT TACTGAGCAG 
GAATTAGAGC TGCTCATATC AGGGCTGCCT ACCATCGACA TTGATGATCT AAAATCTAAC 
ACTGAGTACC ACAAGTACCA ATCCAACTCT ATTCAGATCC AGTGGTTTTG GAGAGCATTG 
CGTTCCTTTG ACCAAGCAGA CCGTGCCAAG TTCCTCCAGT TTGTCACAGG TACTTCCAAG 
GTACCCCTAC AAGGATTTGC TGCCCTTGAA GGTATGAATG GCATTCAGAA GTTTCAGATT 
CATCGAGATG ACAGATCCAC AGATCGCCTG CCTTCAGCTC ACACATGTTT TAATCAACTG 
GACCTGCCTG CCTATGAGAG CTTTGAGAAG CTCCGCCACA TGCTACTATT GGCCATCCAG 
GAATGCTCTG AAGGCTTTGG GCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ubiquitin Ligase Huwe1 Modulates Spermatogenesis by Regulating Spermatogonial Differentiation and Entry into Meiosis.
Scientific reports7(1)17759(2017 Dec)
Bose R,Sheng K,Moawad AR,Manku G,O'Flaherty C,Taketo T,Culty M,Fok KL,Wing SS

Huwe1 Regulates the Establishment and Maintenance of Spermatogonia by Suppressing DNA Damage Response.
Endocrinology158(11)4000-4016(2017 Nov)
Fok KL,Bose R,Sheng K,Chang CW,Katz-Egorov M,Culty M,Su S,Yang M,Ruan YC,Chan HC,Iavarone A,Lasorella A,Cencic R,Pelletier J,Nagano M,Xu W,Wing SS

Huwe1 Sustains Normal Ovarian Epithelial Cell Transformation and Tumor Growth through the Histone H1.3-
Cancer research77(18)4773-4784(2017 Sep)
Yang D,Sun B,Zhang X,Cheng D,Yu X,Yan L,Li L,An S,Jiang H,Lasorella A,Iavarone A,Zhang S,Zou F,Zhao X

TGF?R signalling controls CD103
Nature communications8(1)620(2017 09)
Bain CC,Montgomery J,Scott CL,Kel JM,Girard-Madoux MJH,Martens L,Zangerle-Murray TFP,Ober-Bl?baum J,Lindenbergh-Kortleve D,Samsom JN,Henri S,Lawrence T,Saeys Y,Malissen B,Dalod M,Clausen BE,Mowat AM

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Huwe1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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