HUWE1 cDNA ORF clone, Bos taurus(cattle)

The following HUWE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HUWE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa40699 NM_001110004.1
Latest version!
Bos taurus HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa238331 XM_024987892.1
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa238332 XM_024987900.1
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa35633 XM_010821997.2
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa35633 XM_005228212.3
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa35633 XM_024987894.1
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa35633 XM_024987895.1
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_024987890.1
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_024987891.1
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_010821989.2
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_024987889.1
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_010821990.3
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_010821994.3
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_010821995.2
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_010821992.3
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_015461712.2
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_015461710.2
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_015461711.2
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48120 XM_024987893.1
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48120 XM_010821996.2
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48121 XM_024987896.1
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48121 XM_010821999.2
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48122 XM_024987897.1
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48123 XM_024987898.1
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48124 XM_010822002.2
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48124 XM_024987899.1
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48125 XM_010822003.2
Latest version!
Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OBa48119 XM_010821990.2 Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_010821994.2 Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_015461710.1 Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_015461711.1 Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_010821992.2 Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa48119 XM_015461712.1 Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa35633 XM_005228212.2 Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa40699
    Clone ID Related Accession (Same CDS sequence) NM_001110004.1
    Accession Version NM_001110004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-12
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000030.1. On Oct 19, 2007 this sequence version replaced XM_872867.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

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    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATAA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTCCACTT AGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGATTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT
    GTACTTGTGC GAAACTGTAT CCAGGCCATG ATTGACCCTT CCATGGATCC ATACCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CAAGCTACGA TGCTGGTGGC
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG CCGAAAAGAA TGCCCTTTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTGCACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTCCAGTGT
    ATTCCACAAC GAGCCGCACT TCTCAAATCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA
    GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTCTGCC TACCTCCCTG
    AAACACATCA TCAGCAATGC AGAATACTAT GGCCCATCAC TCTTTCTCCT AGCTACTGAA
    GTGGTGACTG TGTTTGTATT TCAAGAACCA TCATTGCTTT CCTCACTCCA GGACAATGGA
    TTGACAGATG TCATGCTACA TGCACTGCTT ATCAAAGACG TTCCTGCTAC CCGTGAAGTT
    CTTGGCTCCC TCCCAAATGT TTTCAGTGCA CTCTGCTTGA ATGCCCGAGG TCTGCAGTCA
    TTTGTACAGT GTCAGCCTTT TGAACGTCTC TTCAAAGTTC TCCTGTCTCC AGATTACCTC
    CCAGCCATGC GGAGAAGGAG GAGTTCTGAT CCTCTAGGGG ATACTGCCTC CAACCTAGGA
    AGTGCTGTAG ATGAGCTCAT GAGACATCAG CCTACCCTCA AAACAGATGC AACGACTGCG
    ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ACCCTAAATA CATCTGTCAG
    AAGCCATCAA TCCAGAAGGC AGATGGCACT GCCGCTGCTC CTCCCCCAAG ATCTAACCAT
    GCTGCAGAAG AGGCCTCTAG TGAGGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC
    TTTAATTCTA CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTTGTTGG TACAGAGGAG
    CGTATTCCTA TTCCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT
    CTGAGCAACA ATACAACTGA TGACCACTGC CAGGAATTTG TGAATCAGAA AGGACTCTTG
    CCTTTGGTCA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC GTCTGCAGCC
    TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACAC TGTCACATGA ACCCAAAGTC
    CTCCAGGAAG GTCTCCTTCA GTTGGACTCC ATTCTTTCCT CCTTGGAGCC CTTACACCGC
    CCAATAGAAT CCCCCGGGGG CTCAGTGTTG TTGCGAGAAC TGGCTTGTGC TGGCAATGTT
    GCTGATGCTA CCCTCTCAGC TCAAGCCACA CCTCTACTAC ATGCACTCAC TGCTGCCCAT
    GCTTACATCA TGATGTTTGT TCATACTTGC AGGGTTGGAC AGAGTGAAAT TCGTTCCATC
    TCCATAAACC AGTGGGGCTC TCAGTTGGGT CTGAGTGTTC TGAGCAAGCT GAGCCAGTTA
    TACTGTTCCC TGGTATGGGA AAGCACTGTC CTCCTCTCCC TCTGTACCCC AAACAGCTTG
    CCATCTGGGT GTGAATTTGG CCAGGCAGAC ATGCAGAAAC TGGTTCCAAA GGATGAGAAG
    ACAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGGGATC AGGATGGAAC CACTGGAAGT
    ATGGATGCTT CTACCCAGGG CTTGTTGGAA GGCATTGGGC TTGATGGTGA CACACTGGCT
    CCTATGGAGA CAGACGAACC TACTGCTTCA GACTCTAAGG GCAAATCTAA AATCACACCT
    GCCATGGCTG CCAGAATCAA GCAAATCAAG CCATTGTTGT CAGCTTCCTC TAGATTAGGC
    AGAGCACTGG CTGAGCTGTT TGGACTCCTT GTTAAACTTT GTGTGGGGTC TCCTGTCCGC
    CAGAGAAGAA GCCATCATGC TGCCAGCACC ACCACAGCAC CAACACCTGC TGCTCGATCA
    ACGGCCTCAG CCCTCACTAA GCTCCTCACA AAAGGCTTAT CATGGCAGCC TCCACCATAT
    ACGCCTACTC CACGCTTCAG GCTGACCTTC TTCATCTGTT CAGTTGGTTT TACATCCCCA
    ATGCTGTTTG ATGAGAGGAA GTATCCCTAC CACCTCATGC TGCAGAAATT TCTCTGCTCT
    GGAGGCCACA ATGCCCTTTT TGAAACATTC AACTGGGCTC TGTCTATGGG AGGAAAAGTT
    CCTGTTGCTG AGGGATTAGA ACATTCAGAC TTACCTGATG GCACAGGAGA ATTCCTAGAT
    GCCTGGCTAA TGCTGGTGGA GAAGATGGTA AATCCCACCA CTGTGCTTGA ATCTCCACAT
    TCACTGCCTG CCAAATTGCC TGGAGGTGTT CAGAACTTTC CACAGTTCAG TGCCCTCCGC
    TTCCTTGTGG TAACTCAGAA GGCAGCATTT ACTTGCATCA AGAACCTGTG GAACCGGAAA
    CCCTTGAAAG TGTATGGTGG GCGAATGGCT GAGTCCATGC TAGCCATCCT GTGCCACATC
    CTCCGAGGAG AACCTGTGAT TCGAGAGAGA CTGAGCAAAG AAAAGGAGGG ATCTCGAGGA
    GAAGAGGATA CAGGACAAGA AGAAGGTGGC TCTCGCCGGG AACCTCAAGT CAACCAGCAA
    CAACTACAAC AGCTCATGGA CATGGGCTTC ACAAGGGAAC ATGCCATGGA GGCCCTGTTG
    AACACCAGTA CCATGGAGCA GGCCACAGAG TACCTTCTAA CCCACCCTCC CCCGATCATG
    GGAGGAGTTG TTCGGGATCT GAGTATGTCT GAAGAGGACC AGATGATGAG AGCGATTGCC
    ATGTCTCTGG GACAGGATAT CCCAATGGAT CAAAGAGCAG AGTCACCTGA GGAAGTTGCT
    TGCCGAAAGG AAGAGGAGGA ACGGAAAGCT CGAGAAAAGC AGGAAGAGGA AGAGGCTAAA
    TGTCTAGAGA AGTTCCAGGA TGCTGATCCA TTGGAGCAAG ATGAGCTCCA CACTTTCACA
    GATACAATGT TGCCAGGCTG CTTTCACCTT CTTGATGAGT TGCCAGACAC TGTATACCGA
    GTGTGTGATC TGATCATGAC AGCAATCAAA CGAAATGGAG CAGACTATCG AGACATGATT
    CTGAAGCAAG TAGTTAATCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT
    CCCCTGACAA CAAGTGACAC AAAAACTGTA TCAGAGTGGA TCAGTCAGAT GGCCACTCTA
    CCCCAGGCCT CTAATTTGGC CACTAGAATC TTGCTTTTAA CACTGCTTTT TGAGGAATTG
    AAGCTACCTT GTGCTTGGGT GGTTGAATCT AGTGGCATCC TTAATGTCCT AATCAAACTC
    TTGGAAGTGG TTCAGCCCTG CCTACAGGCC GCCAAGGAAC AAAAGGAGGT CCAGACCCCA
    AAGTGGATCA CTCCAGTGTT GCTCCTGATT GATTTCTATG AAAAAACAGC CATTTCCTCA
    AAGAGGAGAG CCCAAATGAC TAAGTATCTA CAGTCTAACA ACAACAACTG GCGCTGGTTT
    GATGACCGCT CTGGACGTTG GTGTAGTTAC AGTGCAAGCA ACAACAGCAC TATTGATTCT
    GCCTGGAAAT CTGGAGAGAC AAGTGTGCGT TTCACTGCAG GCCGAAGAAG ATACACTGTC
    CAGTTCACTA CAATGGTACA GGTTAATGAG GAAACAGGGA ACCGGCGTCC TGTGATGCTG
    ACACTCCTGA GGGTACCCCG ATTGAATAAA AATTCTAAAA ACAGCAATGG ACAGGAACTA
    GAGAAGACAC TGGAAGAAAG CAAAGAAATG GATATCAAAC GTAAAGAAAA TAAAACCAGT
    GATACCCCCT TGGCCCTAGA CAGTACAAAT ACTGAAAAGG AGACAAGCCT GGAAGAAACA
    AAAATTGGGG AGATCTTGAT CCAGGGCCTG ACAGAGGATA TGGTGACTGT TTTAATCCGG
    GCCTGTGTGA GCATGCTAGG CGTCCCTGTG GATCCAGACA CTTTGCATGC CACCCTTCGC
    CTATGCCTGA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA GCTGAAGAGT
    ACTCGCATGA TCTTAAATTT GACCCAGAGT TCAGGCTTCA ATGGGTTTAC TCCCCTGGTT
    ACCCTTCTCT TAAGACACAT CATTGAGGAC CCCTGCACCC TGCGCCATAC CATGGAAAAG
    GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT GGTAGCACTA CTTCTGGTGT TGTATCTGGC
    AGCCTTGGCT CTCGGGAGAT CAACTACATC CTTCGTGTCC TTGGACCAGC CGCATGCCGC
    AACCCAGACA TTTTCACAGA AGTGGCCAAC TGCTGTATCC GCATTGCCCT TCCAGCCCCT
    CGCGGCTCAG GAACTGCTTC AGATGATGAG TTTGAGAATC TCAGAATTAA AGGCCCGAAT
    GCTGTACAGC TGGTGAAAAC AACCCCTTTG AAGCCCTCAC CTCTGCCTGT CATTCCTGAT
    ACTATCAAGG AAGTGATCTA TGACATGCTA AATGCCCTGG CTGCATACCA TGCTCCAGAG
    GAAGCAGACA AATCTGATCC TAAACCAGGG GGTATGACTC AAGAGGTTGG CCAGCTCCTG
    CAAGACATGG GTGATGACGT ATACCAGCAG TACCGGTCAC TTACCCGTCA GAGCAGTGAC
    TTTGATGCAC AGACAGGATT TTCCATTAAT AGTCAGGTCT TTGCTGCAGA TGGTTCCTCC
    ACTGAGACTT CCGCATCTGG GGTATCCCAA GGAGAAGTGT CAACTCCGGA GGAGTCTCGG
    GATGGGAAGA AAGATAAAGA AGGGGACCGG GCCTCTGAGG AAGGCAAGCA GAAAGGCAAG
    GGCAGCAAAC CTTTAATGCC CACCTCCACT ATTCTTCGGC TTCTGGCAGA GTTGGTGAGA
    TCCTATGTTG GTATTGCTAC CCTGATTGCC AACTACAGCT ACACTGTGGG CCAGTCTGAG
    CTGATCAAAG AGGACTGCAG TGTGCTAGCT TTTGTCTTGG ACCACCTCCT CCCGCATACC
    CAGAATGCAG AAGACAAAGA CACACCGGCG CTGGCCCGCC TGTTCCTCGC AAGCCTGGCT
    GCCTCAGGGA GTGGCACAGA TGCCCAGGTG GCCCTTGTGA ATGAAGTAAA AGCAGCCCTG
    GGAAGGGCAC TGGCTATGGC TGAGAGTACA GAGAAACATG CCAGACTTCA GGCAGTGATG
    TGTATCATCA GTACTATCAT GGAGTCCTGC CCCTCCACCT CCAGTTTCTA CAGCAGTGCA
    ACAGCCAAGA CCCAACACAA CGGCATGAAC AACATCATTC GACTCTTCCT GAAGAAGGGA
    CTGGTTAATG ACCTGGCCAG AGTGCCTCAC AGCCTGGACC TGTCCAGTCC CAACATGGCC
    AACACTGTCA ATGCTGCTCT GAAGCCTTTG GAAACACTTT CCCGGATTGT GAACCAGCCC
    AGTAGCCTTT TTGGCAGTAA GAGTGCTTCT AGCAAGAGCA AGTCTGAGCA GGATGCCCAA
    GGAGCTGCTC AGGACTCTAA TAGCAACCAA CAGGACCCAG GCGAGCCTGG GGAAACAGAA
    GTGCAGGAGG AGGATCATGA TGTCACTCAG ACAGAGGTGG CAGATGGGGA TATCATGGAT
    GGGGAGGCTG AAACCGACTC AGTGGTGATT GCTGGGCAGC CTGAGGTGCT CAGTTCACAA
    GAGATGCAGG TTGAGAATGA GCTGGAGGAC CTGATAGATG AGTTGCTTGA GAGGGATGGC
    GGATCTGGGA ACAGTACAAT TATAGTGAGC AGAAGTGGAG AGGATGAATC ACAAGAGGAC
    GTGCTGATGG ATGAAGCTCC TTCCAACCTC AGCCAAGCTT CCACCTTGCA GGCCAACCGA
    GAAGATTCCA TGAATATCCT GGACCCTGAG GATGAGGAGG AGCACACTCA GGAAGAGGAC
    AGCAGTGGCA GTAACGAGGA TGAGGATGAT AGTCAGGATG AAGAGGAGGA GGAGGAGGAA
    GATGAGGAAG ATGATCAGGA GGATGATGAA GGTGAAGAGG GAGATGAAGA CGATGACGAC
    GATGGCTCTG AAATGGAATT GGATGAGGAT TACCCTGATA TGAACGCTTC TCCCTTGGTC
    CGATTTGAGC GCTTTGACCG GGAGGATGAT CTCATCATTG AGTTTGACAA CATGTTCTCC
    AGTGCTACAG ACATCCCCCC ATCCCCAGGA AATATCCCTA CCACCCACCC ACTGATGGTA
    CGCCATGCAG ATCACAGTTC CCTGACACTG GGCAGTGGCT CCTCTACAAC ACGTCTCACC
    CAGGGCATTG GGCGCAGTCA AAGGACCCTG AGACAGCTGA CAGCCAACAC AGGCCATACC
    ATTCACGTTC ACTACCCTGG GAATCGCCAG CCCAATCCTC CTCTTATACT CCAGAGGTTG
    CTTGGTCCCT CTGCTGCTGC TGACATCCTT CAGCTGAGCA GCAGCCTTCC CCTACAAAGC
    CGAGGCCGAG CCCGCCTCCT AGTGGGCAAC GATGACGTCC ACATCATCGC CCGGTCTGAT
    GATGAACTAC TGGATGACTT TTTCCATGAT CAGAGCACAG CTACCAGCCA AGCAGGGACC
    CTATCCAGTA TCCCTACAGC CCTGACTCGC TGGACAGAGG AATGCAAAGT TCTTGATGCT
    GAGAGCATGC ATGACTGTGT TTCAGTTGTT AAAGTGCCCA TTGTCAATCA TCTGGAATTC
    CTGAGGGATG AGGAGCTGGA AGAAAGGAGG GAGAAAAGGA GGAAACAACT AGCTGAGGAA
    GAAACAAAGA TAACCGACAA AGGCAAAGAA GATAAGGAGA ACAGGGATCA GAGTGCACAG
    TGTGCTGCAT CTAAGACAAA TGACTCCACT GAACAGAACC TCTCAGATGG GACTCCTATG
    CCTGACAGCT ACCCAGCAAC CCCATCTTCA ACTGATGTAG CTACATCTGA GCCCAGGGAG
    ACCCTTGTCA CTCTGCAGCC CTCCCAACAG CAACCAACAC TCCCAACCCC ACCAGCCTTG
    GGAGAGATTC CTCAAGAGCT GCAGTCCCCA ATTGGAGAAG GTGGGAGCTC TACACAGCTA
    TTGATGCCTG TAGAGCCAGA GGAATTGGGT CCCACAAGGC CAAGTGGAGA AGCAGAAACA
    ACTCAGATGG AGCTATCTCC AGCTCCCACT ATAACCTCTC TTTCCCCAGA GAGAGCTGAA
    GATTCTGATG CTCTGACAGC TGTCAGCAGT CAACTGGAAG GCTCTCCCAT GGATACTAGC
    AGCCTGGCTT CCTGTACCCT GGAGGAGGCT GTGGGTGACG CCCCAGCAAC TGGCAGTTCT
    GAGCAGCCCA CAGCAGGCAG CTCCACTCCT GGGGATGCCC CACCAGTTGT GACAGAAGTA
    CAAGGCAGGG GTGATGGGTC AGGGGAACCT ACCCAGCCCC CTGAGGACAG CTCTCCCCCT
    GCATCCTCTG AGAGTTCTTC CACCAGAGAT TCCGCGGTGG CCATTTCTGG AGCAGATTCC
    CGGGGAATCC TGGAAGAGCC ATTGCCTTCA ACAAGCAGTG AAGAAGAAGA TCCTCTTGCA
    GGTATTAGTC TCCCCGAAGG TGTGGACCCC TCTTTTCTGG CCGCTCTGCC TGATGACATT
    CGCCGAGAAG TCCTACAGAA CCAGCTGGGC ATTCGTCCAC CAACACGAAC TGCCCCTTCA
    ACAAATAACT CAGCTCCTGC AGTGGTGGGG AATCCTGGTG TCACCGAAGT GAGCCCTGAG
    TTCCTGGCTG CTCTGCCTCC AGCCATCCAG GAGGAAGTAC TGGCACAGCA GAGAGCTGAG
    CAACAGCGAC GGGAACTAGC ACAAAATGCC AGCTCAGACA CCCCCATGGA CCCTGTGACC
    TTCATCCAGA CTCTGCCCTC AGACCTGCGC CGTAGTGTCC TAGAGGACAT GGAGGACAGT
    GTGTTGGCCG TGATGCCACC TGACATCGCA GCTGAAGCTC AAGCCCTGAG GCGAGAGCAA
    GAAGCCCGGC AGAGGCAGCT CATGCATGAG CGACTTTTTG GGCACAGCAG CACCTCTGCA
    CTCTCTGCCA TTCTCCGAAG CCCAGCCTTC ACCAGTCGCC TGAGTGGCAA CCGTGGGGTC
    CAATACACTC GCCTTGCTGT GCAAAGAGGT GGCACCTTCC AGATGGGGGG TAGCAGCAGC
    CATAATAGGC CTTCTGGCAG TAATGTGGAC ACCCTCCTAC GTCTTCGAGG ACGGCTCCTC
    CTGGATCACG AAGCCCTGTC TTGTCTCCTG GTCCTACTTT TTGTGGATGA GCCAAAACTC
    AATACTAGCC GTCTTCACCG AGTACTGAGA AATCTCTGCT ACCATGCCCA GACCCGCCAC
    TGGGTTATCC GTAGCCTGCT TTCCATCTTG CAGCGAAGCA GTGAGAGTGA GCTTTGCATT
    GAAACACCCA AGCTCTCCTC AAGTGAGGAA AAGGGCAAAA AGTCAAGCAA GAGCTGTGGG
    TCAAGCAGCC ATGAGAACCG GCCCCTGGAC CTGCTACACA AGATGGAGTC TAAGAGTTCC
    AACCAGCTTT CCTGGCTCTC AGTATCCATG GATGCAGCCC TAGGCTGCAG GACTAATATA
    TTCCAGATCC AGCGTTCAGG GGGTCGCAAA CATACTGAGA AACATGCGAG TAGTGGCTCC
    ACTGTCCATA TCCACCCCCA GGCTGCTCCT GTTGTCTGCA GACATGTTCT GGATACACTC
    ATTCAATTGG CCAAGGTGTT TCCCAGTCAC TTCACACAGC AGCGGACCAA AGAAACAAAC
    TGTGAGAGTG ATCGGGAGAG GGGCAGTAAG GCCTGCAGCC CATGCTCTTC ACAGTCCACA
    AGCAGTGGCA TTTGCACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTC
    AGCCGGAAAG GCAAGAACTC CGTGAAGTCA GTGCCAGTGA GCGCTGGTGG TGAGGGGGAA
    ACCTCCCCAT ACAGCCTCGA AGCTTCTCCA CTGGGGCAAC TCATGAACAT GTTGTCACAC
    CCAGTCATCC GCCGGAGCTC TCTCTTAACT GAGAAGCTCC TCAGACTTCT TTCTCTCATC
    TCAATTGCTC TCCCAGAAAA CAAAGTGTCG GAAGCACAGG CTAATTCTGG CAGCAGTGCT
    TCCTCCACCA CTGTTGCCAC TTCAACCACA TCTACCACCA CTATCAGTGC CACCTCCAGC
    ACATCTACTC TGCCTGCTGC AACCACCCCT GTCACTTCTG TTCCAGCCCT GGTTGCTGCC
    ACGGCTATAT CCACCATTGC CGTAGCCGCT TCAACCACAG TGACTACCCC CACAACTGCT
    ACCACTACTG TTTCAACTGC TACTACTACT AAGGCCAGCA AATCTCCAGC TAAAGTTGGT
    GATATCGGCA GCAGCAGCAG CACAGACTTT AAGATGGTAT CCTCTGGCCT CACTGAAAAT
    CAGCTCCAGC TCTCTGTGGA AGTGTTGACA TCCCATTCTT GTTCTGAAGA AGGCCTAGAA
    GATGCAGCAA ATGTGTTACT GCAGCTCTCT CGGGGGGACC CTGGGACCCG GGACACTGTT
    TTGAAGTTGC TACTGAATGG AGCCCGCCAT CTGGGTTATA CGCTTTGTAA ACAGATAGGA
    ACTCTTCTGG CTGAGCTACG GGAATACAAT CTGGAACAGC AGCGGCGAGC CCAGTGTGAA
    ACCCTCTCCC CTGATGGCAT GCCTGAGGAA CAGCCACAGA CCACCAAGCT GAAAGGCAAA
    ATGCAGAGCA GGTTTGACGT GGCTGAGAAT GTGGTAATCG TGGCATCTCA GAAGCGGCCT
    CTGGGTGGCC GGGAGCTCCA GCTGCCTTCT ATGTCCATGT TGACATCCAA GACATCTACC
    CAGAAGTTCT TCTTGAGGGT ACTGCAGGTC ATCATCCAGC TCCGGGATGA CACGCGCCGT
    GCTAACAAGA AGGCCAAGCA GACAGGCAGG CTAGGTTCCT CCGGTTTAGG CTCAGCTAGC
    AGCATCCAGG CAGCTGTTCG GCAGCTGGAG GCTGAGGCTG ATGCCATTAT ACAAATGGTA
    CGTGAGGGTC AGAGGGCGCG GAGACAGCAA CAAGCAGCAA CGTCGGAGTC AAGCCAGTCA
    GAGGCATCTG TCCGAAGGGA GGAGTCACCC ATGGATGTGG ACCAGCCATC TCCCAGTGCT
    CAAGATACTC AGTCCATTGG CTCAGATGGA ACTCCACAGG GAGAGAAGGA GAAGGAGGAG
    AGGCCACCTG AGTTACCCCT GCTCAGTGAG CAGCTGAACT TGGATGAGCT GTGGGACATG
    CTGGGAGAGT GTCTAAAGGA ACTGGAGGAA TCCCATGACC AGCATGCGGT GCTAGTGCTA
    CAACCTGCTG TCGAGGCCTT CTTTCTGGTC CATGCAACAG AGCGGGAGAG CAAGCCTCCT
    GTCCGGGACA CCCGTGAGAG CCAGCTGGCA CACATCAAGG ACGAGCCACC TCCACTCTCC
    CCTGCCCCTT TAACCCCAGC CACTCCTTCC TCCCTTGACC CGTTCTTCTC CCGGGAGCCC
    TCATCTATGC ACATCTCCTC AAGCCTGCCC CCTGACACAC AGAAGTTTCT TCGTTTTGCA
    GAGACTCATC GCACTGTGTT AAACCAGATC CTACGGCAGT CCACCACCCA CCTCGCTGAT
    GGGCCTTTTG CTGTCCTGGT AGACTACATT CGTGTCCTCG ACTTCGATGT CAAGCGCAAA
    TATTTCCGCC AAGAGCTGGA GCGTTTGGAC GAGGGACTCC GGAAAGAAGA CATGGCTGTG
    CACGTTCGTC GTGATCATGT CTTTGAAGAC TCCTATCGTG AGCTGCACCG GAAATCCCCT
    GAAGAAATGA AGAATCGATT GTATATAGTA TTTGAAGGAG AAGAAGGGCA GGATGCTGGA
    GGGCTCCTGC GGGAGTGGTA TATGATCATC TCTCGAGAGA TGTTTAACCC TATGTATGCC
    TTGTTCCGTA CCTCACCTGG TGATCGGGTC ACATACACCA TCAATCCATC TTCTCACTGC
    AACCCCAACC ACCTCAGCTA CTTCAAGTTT GTTGGACGCA TCGTGGCCAA AGCTGTGTAT
    GACAACCGCC TCCTGGAGTG CTACTTTACT AGGTCCTTCT ACAAACACAT TTTGGGCAAG
    TCTGTCAGAT ATACAGATAT GGAGAGTGAA GACTACCACT TTTACCAGGG CCTGGTTTAT
    CTGCTGGAAA ATGATGTCTC CACACTAGGC TATGATCTCA CTTTCAGCAC TGAGGTCCAA
    GAGTTTGGAG TCTGTGAAGT TCGTGACCTC AAACCCAATG GGGCCAACAT CTTGGTAACA
    GAGGAAAATA AGAAGGAATA TGTACACCTG GTGTGCCAGA TGAGAATGAC AGGAGCCATC
    CGCAAACAGC TGGCGGCTTT CTTAGAAGGT TTCTATGAGA TCATTCCAAA GCGCCTCATT
    TCCATCTTCA CTGAGCAGGA GTTAGAGCTG CTCATATCAG GACTGCCCAC AATCGACATT
    GACGATTTGA AATCTAACAC TGAATATCAC AAGTACCAGT CCAACTCTAT TCAGATCCAG
    TGGTTCTGGA GAGCACTGCG TTCTTTCGAT CAAGCTGACC GTGCCAAGTT CCTCCAGTTT
    GTCACAGGTA CTTCCAAGGT GCCCCTGCAA GGTTTTGCTG CCCTTGAAGG CATGAATGGC
    ATTCAGAAGT TTCAAATCCA TCGAGATGAC AGGTCCACAG ACCGCCTGCC TTCAGCTCAC
    ACATGTTTTA ATCAGCTGGA CCTGCCTGCC TATGAGAGCT TTGAGAAGCT CCGCCACATG
    CTACTATTGG CCATCCAGGA GTGCTCTGAA GGCTTTGGGC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001103474.1
    CDS331..13458
    Translation

    Target ORF information:

    RefSeq Version NM_001110004.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001110004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATAA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTCCACTT AGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGATTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT
    GTACTTGTGC GAAACTGTAT CCAGGCCATG ATTGACCCTT CCATGGATCC ATACCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CAAGCTACGA TGCTGGTGGC
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG CCGAAAAGAA TGCCCTTTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTGCACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGG AGTCCAGTGT
    ATTCCACAAC GAGCCGCACT TCTCAAATCC ATGTTGAATT TCCTCAAGAA GGCCATTCAA
    GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTCTGCC TACCTCCCTG
    AAACACATCA TCAGCAATGC AGAATACTAT GGCCCATCAC TCTTTCTCCT AGCTACTGAA
    GTGGTGACTG TGTTTGTATT TCAAGAACCA TCATTGCTTT CCTCACTCCA GGACAATGGA
    TTGACAGATG TCATGCTACA TGCACTGCTT ATCAAAGACG TTCCTGCTAC CCGTGAAGTT
    CTTGGCTCCC TCCCAAATGT TTTCAGTGCA CTCTGCTTGA ATGCCCGAGG TCTGCAGTCA
    TTTGTACAGT GTCAGCCTTT TGAACGTCTC TTCAAAGTTC TCCTGTCTCC AGATTACCTC
    CCAGCCATGC GGAGAAGGAG GAGTTCTGAT CCTCTAGGGG ATACTGCCTC CAACCTAGGA
    AGTGCTGTAG ATGAGCTCAT GAGACATCAG CCTACCCTCA AAACAGATGC AACGACTGCG
    ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGAG ACCCTAAATA CATCTGTCAG
    AAGCCATCAA TCCAGAAGGC AGATGGCACT GCCGCTGCTC CTCCCCCAAG ATCTAACCAT
    GCTGCAGAAG AGGCCTCTAG TGAGGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC
    TTTAATTCTA CCCAGCAGAA TGAAACTGAG CCTAATCAGC AGGTTGTTGG TACAGAGGAG
    CGTATTCCTA TTCCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT
    CTGAGCAACA ATACAACTGA TGACCACTGC CAGGAATTTG TGAATCAGAA AGGACTCTTG
    CCTTTGGTCA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC GTCTGCAGCC
    TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACAC TGTCACATGA ACCCAAAGTC
    CTCCAGGAAG GTCTCCTTCA GTTGGACTCC ATTCTTTCCT CCTTGGAGCC CTTACACCGC
    CCAATAGAAT CCCCCGGGGG CTCAGTGTTG TTGCGAGAAC TGGCTTGTGC TGGCAATGTT
    GCTGATGCTA CCCTCTCAGC TCAAGCCACA CCTCTACTAC ATGCACTCAC TGCTGCCCAT
    GCTTACATCA TGATGTTTGT TCATACTTGC AGGGTTGGAC AGAGTGAAAT TCGTTCCATC
    TCCATAAACC AGTGGGGCTC TCAGTTGGGT CTGAGTGTTC TGAGCAAGCT GAGCCAGTTA
    TACTGTTCCC TGGTATGGGA AAGCACTGTC CTCCTCTCCC TCTGTACCCC AAACAGCTTG
    CCATCTGGGT GTGAATTTGG CCAGGCAGAC ATGCAGAAAC TGGTTCCAAA GGATGAGAAG
    ACAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGGGATC AGGATGGAAC CACTGGAAGT
    ATGGATGCTT CTACCCAGGG CTTGTTGGAA GGCATTGGGC TTGATGGTGA CACACTGGCT
    CCTATGGAGA CAGACGAACC TACTGCTTCA GACTCTAAGG GCAAATCTAA AATCACACCT
    GCCATGGCTG CCAGAATCAA GCAAATCAAG CCATTGTTGT CAGCTTCCTC TAGATTAGGC
    AGAGCACTGG CTGAGCTGTT TGGACTCCTT GTTAAACTTT GTGTGGGGTC TCCTGTCCGC
    CAGAGAAGAA GCCATCATGC TGCCAGCACC ACCACAGCAC CAACACCTGC TGCTCGATCA
    ACGGCCTCAG CCCTCACTAA GCTCCTCACA AAAGGCTTAT CATGGCAGCC TCCACCATAT
    ACGCCTACTC CACGCTTCAG GCTGACCTTC TTCATCTGTT CAGTTGGTTT TACATCCCCA
    ATGCTGTTTG ATGAGAGGAA GTATCCCTAC CACCTCATGC TGCAGAAATT TCTCTGCTCT
    GGAGGCCACA ATGCCCTTTT TGAAACATTC AACTGGGCTC TGTCTATGGG AGGAAAAGTT
    CCTGTTGCTG AGGGATTAGA ACATTCAGAC TTACCTGATG GCACAGGAGA ATTCCTAGAT
    GCCTGGCTAA TGCTGGTGGA GAAGATGGTA AATCCCACCA CTGTGCTTGA ATCTCCACAT
    TCACTGCCTG CCAAATTGCC TGGAGGTGTT CAGAACTTTC CACAGTTCAG TGCCCTCCGC
    TTCCTTGTGG TAACTCAGAA GGCAGCATTT ACTTGCATCA AGAACCTGTG GAACCGGAAA
    CCCTTGAAAG TGTATGGTGG GCGAATGGCT GAGTCCATGC TAGCCATCCT GTGCCACATC
    CTCCGAGGAG AACCTGTGAT TCGAGAGAGA CTGAGCAAAG AAAAGGAGGG ATCTCGAGGA
    GAAGAGGATA CAGGACAAGA AGAAGGTGGC TCTCGCCGGG AACCTCAAGT CAACCAGCAA
    CAACTACAAC AGCTCATGGA CATGGGCTTC ACAAGGGAAC ATGCCATGGA GGCCCTGTTG
    AACACCAGTA CCATGGAGCA GGCCACAGAG TACCTTCTAA CCCACCCTCC CCCGATCATG
    GGAGGAGTTG TTCGGGATCT GAGTATGTCT GAAGAGGACC AGATGATGAG AGCGATTGCC
    ATGTCTCTGG GACAGGATAT CCCAATGGAT CAAAGAGCAG AGTCACCTGA GGAAGTTGCT
    TGCCGAAAGG AAGAGGAGGA ACGGAAAGCT CGAGAAAAGC AGGAAGAGGA AGAGGCTAAA
    TGTCTAGAGA AGTTCCAGGA TGCTGATCCA TTGGAGCAAG ATGAGCTCCA CACTTTCACA
    GATACAATGT TGCCAGGCTG CTTTCACCTT CTTGATGAGT TGCCAGACAC TGTATACCGA
    GTGTGTGATC TGATCATGAC AGCAATCAAA CGAAATGGAG CAGACTATCG AGACATGATT
    CTGAAGCAAG TAGTTAATCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT
    CCCCTGACAA CAAGTGACAC AAAAACTGTA TCAGAGTGGA TCAGTCAGAT GGCCACTCTA
    CCCCAGGCCT CTAATTTGGC CACTAGAATC TTGCTTTTAA CACTGCTTTT TGAGGAATTG
    AAGCTACCTT GTGCTTGGGT GGTTGAATCT AGTGGCATCC TTAATGTCCT AATCAAACTC
    TTGGAAGTGG TTCAGCCCTG CCTACAGGCC GCCAAGGAAC AAAAGGAGGT CCAGACCCCA
    AAGTGGATCA CTCCAGTGTT GCTCCTGATT GATTTCTATG AAAAAACAGC CATTTCCTCA
    AAGAGGAGAG CCCAAATGAC TAAGTATCTA CAGTCTAACA ACAACAACTG GCGCTGGTTT
    GATGACCGCT CTGGACGTTG GTGTAGTTAC AGTGCAAGCA ACAACAGCAC TATTGATTCT
    GCCTGGAAAT CTGGAGAGAC AAGTGTGCGT TTCACTGCAG GCCGAAGAAG ATACACTGTC
    CAGTTCACTA CAATGGTACA GGTTAATGAG GAAACAGGGA ACCGGCGTCC TGTGATGCTG
    ACACTCCTGA GGGTACCCCG ATTGAATAAA AATTCTAAAA ACAGCAATGG ACAGGAACTA
    GAGAAGACAC TGGAAGAAAG CAAAGAAATG GATATCAAAC GTAAAGAAAA TAAAACCAGT
    GATACCCCCT TGGCCCTAGA CAGTACAAAT ACTGAAAAGG AGACAAGCCT GGAAGAAACA
    AAAATTGGGG AGATCTTGAT CCAGGGCCTG ACAGAGGATA TGGTGACTGT TTTAATCCGG
    GCCTGTGTGA GCATGCTAGG CGTCCCTGTG GATCCAGACA CTTTGCATGC CACCCTTCGC
    CTATGCCTGA GGCTCACCCG AGACCACAAA TATGCCATGA TGTTTGCAGA GCTGAAGAGT
    ACTCGCATGA TCTTAAATTT GACCCAGAGT TCAGGCTTCA ATGGGTTTAC TCCCCTGGTT
    ACCCTTCTCT TAAGACACAT CATTGAGGAC CCCTGCACCC TGCGCCATAC CATGGAAAAG
    GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT GGTAGCACTA CTTCTGGTGT TGTATCTGGC
    AGCCTTGGCT CTCGGGAGAT CAACTACATC CTTCGTGTCC TTGGACCAGC CGCATGCCGC
    AACCCAGACA TTTTCACAGA AGTGGCCAAC TGCTGTATCC GCATTGCCCT TCCAGCCCCT
    CGCGGCTCAG GAACTGCTTC AGATGATGAG TTTGAGAATC TCAGAATTAA AGGCCCGAAT
    GCTGTACAGC TGGTGAAAAC AACCCCTTTG AAGCCCTCAC CTCTGCCTGT CATTCCTGAT
    ACTATCAAGG AAGTGATCTA TGACATGCTA AATGCCCTGG CTGCATACCA TGCTCCAGAG
    GAAGCAGACA AATCTGATCC TAAACCAGGG GGTATGACTC AAGAGGTTGG CCAGCTCCTG
    CAAGACATGG GTGATGACGT ATACCAGCAG TACCGGTCAC TTACCCGTCA GAGCAGTGAC
    TTTGATGCAC AGACAGGATT TTCCATTAAT AGTCAGGTCT TTGCTGCAGA TGGTTCCTCC
    ACTGAGACTT CCGCATCTGG GGTATCCCAA GGAGAAGTGT CAACTCCGGA GGAGTCTCGG
    GATGGGAAGA AAGATAAAGA AGGGGACCGG GCCTCTGAGG AAGGCAAGCA GAAAGGCAAG
    GGCAGCAAAC CTTTAATGCC CACCTCCACT ATTCTTCGGC TTCTGGCAGA GTTGGTGAGA
    TCCTATGTTG GTATTGCTAC CCTGATTGCC AACTACAGCT ACACTGTGGG CCAGTCTGAG
    CTGATCAAAG AGGACTGCAG TGTGCTAGCT TTTGTCTTGG ACCACCTCCT CCCGCATACC
    CAGAATGCAG AAGACAAAGA CACACCGGCG CTGGCCCGCC TGTTCCTCGC AAGCCTGGCT
    GCCTCAGGGA GTGGCACAGA TGCCCAGGTG GCCCTTGTGA ATGAAGTAAA AGCAGCCCTG
    GGAAGGGCAC TGGCTATGGC TGAGAGTACA GAGAAACATG CCAGACTTCA GGCAGTGATG
    TGTATCATCA GTACTATCAT GGAGTCCTGC CCCTCCACCT CCAGTTTCTA CAGCAGTGCA
    ACAGCCAAGA CCCAACACAA CGGCATGAAC AACATCATTC GACTCTTCCT GAAGAAGGGA
    CTGGTTAATG ACCTGGCCAG AGTGCCTCAC AGCCTGGACC TGTCCAGTCC CAACATGGCC
    AACACTGTCA ATGCTGCTCT GAAGCCTTTG GAAACACTTT CCCGGATTGT GAACCAGCCC
    AGTAGCCTTT TTGGCAGTAA GAGTGCTTCT AGCAAGAGCA AGTCTGAGCA GGATGCCCAA
    GGAGCTGCTC AGGACTCTAA TAGCAACCAA CAGGACCCAG GCGAGCCTGG GGAAACAGAA
    GTGCAGGAGG AGGATCATGA TGTCACTCAG ACAGAGGTGG CAGATGGGGA TATCATGGAT
    GGGGAGGCTG AAACCGACTC AGTGGTGATT GCTGGGCAGC CTGAGGTGCT CAGTTCACAA
    GAGATGCAGG TTGAGAATGA GCTGGAGGAC CTGATAGATG AGTTGCTTGA GAGGGATGGC
    GGATCTGGGA ACAGTACAAT TATAGTGAGC AGAAGTGGAG AGGATGAATC ACAAGAGGAC
    GTGCTGATGG ATGAAGCTCC TTCCAACCTC AGCCAAGCTT CCACCTTGCA GGCCAACCGA
    GAAGATTCCA TGAATATCCT GGACCCTGAG GATGAGGAGG AGCACACTCA GGAAGAGGAC
    AGCAGTGGCA GTAACGAGGA TGAGGATGAT AGTCAGGATG AAGAGGAGGA GGAGGAGGAA
    GATGAGGAAG ATGATCAGGA GGATGATGAA GGTGAAGAGG GAGATGAAGA CGATGACGAC
    GATGGCTCTG AAATGGAATT GGATGAGGAT TACCCTGATA TGAACGCTTC TCCCTTGGTC
    CGATTTGAGC GCTTTGACCG GGAGGATGAT CTCATCATTG AGTTTGACAA CATGTTCTCC
    AGTGCTACAG ACATCCCCCC ATCCCCAGGA AATATCCCTA CCACCCACCC ACTGATGGTA
    CGCCATGCAG ATCACAGTTC CCTGACACTG GGCAGTGGCT CCTCTACAAC ACGTCTCACC
    CAGGGCATTG GGCGCAGTCA AAGGACCCTG AGACAGCTGA CAGCCAACAC AGGCCATACC
    ATTCACGTTC ACTACCCTGG GAATCGCCAG CCCAATCCTC CTCTTATACT CCAGAGGTTG
    CTTGGTCCCT CTGCTGCTGC TGACATCCTT CAGCTGAGCA GCAGCCTTCC CCTACAAAGC
    CGAGGCCGAG CCCGCCTCCT AGTGGGCAAC GATGACGTCC ACATCATCGC CCGGTCTGAT
    GATGAACTAC TGGATGACTT TTTCCATGAT CAGAGCACAG CTACCAGCCA AGCAGGGACC
    CTATCCAGTA TCCCTACAGC CCTGACTCGC TGGACAGAGG AATGCAAAGT TCTTGATGCT
    GAGAGCATGC ATGACTGTGT TTCAGTTGTT AAAGTGCCCA TTGTCAATCA TCTGGAATTC
    CTGAGGGATG AGGAGCTGGA AGAAAGGAGG GAGAAAAGGA GGAAACAACT AGCTGAGGAA
    GAAACAAAGA TAACCGACAA AGGCAAAGAA GATAAGGAGA ACAGGGATCA GAGTGCACAG
    TGTGCTGCAT CTAAGACAAA TGACTCCACT GAACAGAACC TCTCAGATGG GACTCCTATG
    CCTGACAGCT ACCCAGCAAC CCCATCTTCA ACTGATGTAG CTACATCTGA GCCCAGGGAG
    ACCCTTGTCA CTCTGCAGCC CTCCCAACAG CAACCAACAC TCCCAACCCC ACCAGCCTTG
    GGAGAGATTC CTCAAGAGCT GCAGTCCCCA ATTGGAGAAG GTGGGAGCTC TACACAGCTA
    TTGATGCCTG TAGAGCCAGA GGAATTGGGT CCCACAAGGC CAAGTGGAGA AGCAGAAACA
    ACTCAGATGG AGCTATCTCC AGCTCCCACT ATAACCTCTC TTTCCCCAGA GAGAGCTGAA
    GATTCTGATG CTCTGACAGC TGTCAGCAGT CAACTGGAAG GCTCTCCCAT GGATACTAGC
    AGCCTGGCTT CCTGTACCCT GGAGGAGGCT GTGGGTGACG CCCCAGCAAC TGGCAGTTCT
    GAGCAGCCCA CAGCAGGCAG CTCCACTCCT GGGGATGCCC CACCAGTTGT GACAGAAGTA
    CAAGGCAGGG GTGATGGGTC AGGGGAACCT ACCCAGCCCC CTGAGGACAG CTCTCCCCCT
    GCATCCTCTG AGAGTTCTTC CACCAGAGAT TCCGCGGTGG CCATTTCTGG AGCAGATTCC
    CGGGGAATCC TGGAAGAGCC ATTGCCTTCA ACAAGCAGTG AAGAAGAAGA TCCTCTTGCA
    GGTATTAGTC TCCCCGAAGG TGTGGACCCC TCTTTTCTGG CCGCTCTGCC TGATGACATT
    CGCCGAGAAG TCCTACAGAA CCAGCTGGGC ATTCGTCCAC CAACACGAAC TGCCCCTTCA
    ACAAATAACT CAGCTCCTGC AGTGGTGGGG AATCCTGGTG TCACCGAAGT GAGCCCTGAG
    TTCCTGGCTG CTCTGCCTCC AGCCATCCAG GAGGAAGTAC TGGCACAGCA GAGAGCTGAG
    CAACAGCGAC GGGAACTAGC ACAAAATGCC AGCTCAGACA CCCCCATGGA CCCTGTGACC
    TTCATCCAGA CTCTGCCCTC AGACCTGCGC CGTAGTGTCC TAGAGGACAT GGAGGACAGT
    GTGTTGGCCG TGATGCCACC TGACATCGCA GCTGAAGCTC AAGCCCTGAG GCGAGAGCAA
    GAAGCCCGGC AGAGGCAGCT CATGCATGAG CGACTTTTTG GGCACAGCAG CACCTCTGCA
    CTCTCTGCCA TTCTCCGAAG CCCAGCCTTC ACCAGTCGCC TGAGTGGCAA CCGTGGGGTC
    CAATACACTC GCCTTGCTGT GCAAAGAGGT GGCACCTTCC AGATGGGGGG TAGCAGCAGC
    CATAATAGGC CTTCTGGCAG TAATGTGGAC ACCCTCCTAC GTCTTCGAGG ACGGCTCCTC
    CTGGATCACG AAGCCCTGTC TTGTCTCCTG GTCCTACTTT TTGTGGATGA GCCAAAACTC
    AATACTAGCC GTCTTCACCG AGTACTGAGA AATCTCTGCT ACCATGCCCA GACCCGCCAC
    TGGGTTATCC GTAGCCTGCT TTCCATCTTG CAGCGAAGCA GTGAGAGTGA GCTTTGCATT
    GAAACACCCA AGCTCTCCTC AAGTGAGGAA AAGGGCAAAA AGTCAAGCAA GAGCTGTGGG
    TCAAGCAGCC ATGAGAACCG GCCCCTGGAC CTGCTACACA AGATGGAGTC TAAGAGTTCC
    AACCAGCTTT CCTGGCTCTC AGTATCCATG GATGCAGCCC TAGGCTGCAG GACTAATATA
    TTCCAGATCC AGCGTTCAGG GGGTCGCAAA CATACTGAGA AACATGCGAG TAGTGGCTCC
    ACTGTCCATA TCCACCCCCA GGCTGCTCCT GTTGTCTGCA GACATGTTCT GGATACACTC
    ATTCAATTGG CCAAGGTGTT TCCCAGTCAC TTCACACAGC AGCGGACCAA AGAAACAAAC
    TGTGAGAGTG ATCGGGAGAG GGGCAGTAAG GCCTGCAGCC CATGCTCTTC ACAGTCCACA
    AGCAGTGGCA TTTGCACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTC
    AGCCGGAAAG GCAAGAACTC CGTGAAGTCA GTGCCAGTGA GCGCTGGTGG TGAGGGGGAA
    ACCTCCCCAT ACAGCCTCGA AGCTTCTCCA CTGGGGCAAC TCATGAACAT GTTGTCACAC
    CCAGTCATCC GCCGGAGCTC TCTCTTAACT GAGAAGCTCC TCAGACTTCT TTCTCTCATC
    TCAATTGCTC TCCCAGAAAA CAAAGTGTCG GAAGCACAGG CTAATTCTGG CAGCAGTGCT
    TCCTCCACCA CTGTTGCCAC TTCAACCACA TCTACCACCA CTATCAGTGC CACCTCCAGC
    ACATCTACTC TGCCTGCTGC AACCACCCCT GTCACTTCTG TTCCAGCCCT GGTTGCTGCC
    ACGGCTATAT CCACCATTGC CGTAGCCGCT TCAACCACAG TGACTACCCC CACAACTGCT
    ACCACTACTG TTTCAACTGC TACTACTACT AAGGCCAGCA AATCTCCAGC TAAAGTTGGT
    GATATCGGCA GCAGCAGCAG CACAGACTTT AAGATGGTAT CCTCTGGCCT CACTGAAAAT
    CAGCTCCAGC TCTCTGTGGA AGTGTTGACA TCCCATTCTT GTTCTGAAGA AGGCCTAGAA
    GATGCAGCAA ATGTGTTACT GCAGCTCTCT CGGGGGGACC CTGGGACCCG GGACACTGTT
    TTGAAGTTGC TACTGAATGG AGCCCGCCAT CTGGGTTATA CGCTTTGTAA ACAGATAGGA
    ACTCTTCTGG CTGAGCTACG GGAATACAAT CTGGAACAGC AGCGGCGAGC CCAGTGTGAA
    ACCCTCTCCC CTGATGGCAT GCCTGAGGAA CAGCCACAGA CCACCAAGCT GAAAGGCAAA
    ATGCAGAGCA GGTTTGACGT GGCTGAGAAT GTGGTAATCG TGGCATCTCA GAAGCGGCCT
    CTGGGTGGCC GGGAGCTCCA GCTGCCTTCT ATGTCCATGT TGACATCCAA GACATCTACC
    CAGAAGTTCT TCTTGAGGGT ACTGCAGGTC ATCATCCAGC TCCGGGATGA CACGCGCCGT
    GCTAACAAGA AGGCCAAGCA GACAGGCAGG CTAGGTTCCT CCGGTTTAGG CTCAGCTAGC
    AGCATCCAGG CAGCTGTTCG GCAGCTGGAG GCTGAGGCTG ATGCCATTAT ACAAATGGTA
    CGTGAGGGTC AGAGGGCGCG GAGACAGCAA CAAGCAGCAA CGTCGGAGTC AAGCCAGTCA
    GAGGCATCTG TCCGAAGGGA GGAGTCACCC ATGGATGTGG ACCAGCCATC TCCCAGTGCT
    CAAGATACTC AGTCCATTGG CTCAGATGGA ACTCCACAGG GAGAGAAGGA GAAGGAGGAG
    AGGCCACCTG AGTTACCCCT GCTCAGTGAG CAGCTGAACT TGGATGAGCT GTGGGACATG
    CTGGGAGAGT GTCTAAAGGA ACTGGAGGAA TCCCATGACC AGCATGCGGT GCTAGTGCTA
    CAACCTGCTG TCGAGGCCTT CTTTCTGGTC CATGCAACAG AGCGGGAGAG CAAGCCTCCT
    GTCCGGGACA CCCGTGAGAG CCAGCTGGCA CACATCAAGG ACGAGCCACC TCCACTCTCC
    CCTGCCCCTT TAACCCCAGC CACTCCTTCC TCCCTTGACC CGTTCTTCTC CCGGGAGCCC
    TCATCTATGC ACATCTCCTC AAGCCTGCCC CCTGACACAC AGAAGTTTCT TCGTTTTGCA
    GAGACTCATC GCACTGTGTT AAACCAGATC CTACGGCAGT CCACCACCCA CCTCGCTGAT
    GGGCCTTTTG CTGTCCTGGT AGACTACATT CGTGTCCTCG ACTTCGATGT CAAGCGCAAA
    TATTTCCGCC AAGAGCTGGA GCGTTTGGAC GAGGGACTCC GGAAAGAAGA CATGGCTGTG
    CACGTTCGTC GTGATCATGT CTTTGAAGAC TCCTATCGTG AGCTGCACCG GAAATCCCCT
    GAAGAAATGA AGAATCGATT GTATATAGTA TTTGAAGGAG AAGAAGGGCA GGATGCTGGA
    GGGCTCCTGC GGGAGTGGTA TATGATCATC TCTCGAGAGA TGTTTAACCC TATGTATGCC
    TTGTTCCGTA CCTCACCTGG TGATCGGGTC ACATACACCA TCAATCCATC TTCTCACTGC
    AACCCCAACC ACCTCAGCTA CTTCAAGTTT GTTGGACGCA TCGTGGCCAA AGCTGTGTAT
    GACAACCGCC TCCTGGAGTG CTACTTTACT AGGTCCTTCT ACAAACACAT TTTGGGCAAG
    TCTGTCAGAT ATACAGATAT GGAGAGTGAA GACTACCACT TTTACCAGGG CCTGGTTTAT
    CTGCTGGAAA ATGATGTCTC CACACTAGGC TATGATCTCA CTTTCAGCAC TGAGGTCCAA
    GAGTTTGGAG TCTGTGAAGT TCGTGACCTC AAACCCAATG GGGCCAACAT CTTGGTAACA
    GAGGAAAATA AGAAGGAATA TGTACACCTG GTGTGCCAGA TGAGAATGAC AGGAGCCATC
    CGCAAACAGC TGGCGGCTTT CTTAGAAGGT TTCTATGAGA TCATTCCAAA GCGCCTCATT
    TCCATCTTCA CTGAGCAGGA GTTAGAGCTG CTCATATCAG GACTGCCCAC AATCGACATT
    GACGATTTGA AATCTAACAC TGAATATCAC AAGTACCAGT CCAACTCTAT TCAGATCCAG
    TGGTTCTGGA GAGCACTGCG TTCTTTCGAT CAAGCTGACC GTGCCAAGTT CCTCCAGTTT
    GTCACAGGTA CTTCCAAGGT GCCCCTGCAA GGTTTTGCTG CCCTTGAAGG CATGAATGGC
    ATTCAGAAGT TTCAAATCCA TCGAGATGAC AGGTCCACAG ACCGCCTGCC TTCAGCTCAC
    ACATGTTTTA ATCAGCTGGA CCTGCCTGCC TATGAGAGCT TTGAGAAGCT CCGCCACATG
    CTACTATTGG CCATCCAGGA GTGCTCTGAA GGCTTTGGGC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238331
    Clone ID Related Accession (Same CDS sequence) XM_024987892.1
    Accession Version XM_024987892.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13272bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
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    2161
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    2641
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    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GACAAATCTG ATCCTAAACC AGGGGGTATG
    ACTCAAGAGG TTGGCCAGCT CCTGCAAGAC ATGGGTGATG ACGTATACCA GCAGTACCGG
    TCACTTACCC GTCAGAGCAG TGACTTTGAT GCACAGACAG GATTTTCCAT TAATAGTCAG
    GTCTTTGCTG CAGATGGTTC CTCCACTGAG ACTTCCGCAT CTGGGGTATC CCAAGGAGAA
    GTGTCAACTC CGGAGGAGTC TCGGGATGGG AAGAAAGATA AAGAAGGGGA CCGGGCCTCT
    GAGGAAGGCA AGCAGAAAGG CAAGGGCAGC AAACCTTTAA TGCCCACCTC CACTATTCTT
    CGGCTTCTGG CAGAGTTGGT GAGATCCTAT GTTGGTATTG CTACCCTGAT TGCCAACTAC
    AGCTACACTG TGGGCCAGTC TGAGCTGATC AAAGAGGACT GCAGTGTGCT AGCTTTTGTC
    TTGGACCACC TCCTCCCGCA TACCCAGAAT GCAGAAGACA AAGACACACC GGCGCTGGCC
    CGCCTGTTCC TCGCAAGCCT GGCTGCCTCA GGGAGTGGCA CAGATGCCCA GGTGGCCCTT
    GTGAATGAAG TAAAAGCAGC CCTGGGAAGG GCACTGGCTA TGGCTGAGAG TACAGAGAAA
    CATGCCAGAC TTCAGGCAGT GATGTGTATC ATCAGTACTA TCATGGAGTC CTGCCCCTCC
    ACCTCCAGTT TCTACAGCAG TGCAACAGCC AAGACCCAAC ACAACGGCAT GAACAACATC
    ATTCGACTCT TCCTGAAGAA GGGACTGGTT AATGACCTGG CCAGAGTGCC TCACAGCCTG
    GACCTGTCCA GTCCCAACAT GGCCAACACT GTCAATGCTG CTCTGAAGCC TTTGGAAACA
    CTTTCCCGGA TTGTGAACCA GCCCAGTAGC CTTTTTGGCA GTAAGAGTGC TTCTAGCAAG
    AGCAAGTCTG AGCAGGATGC CCAAGGAGCT GCTCAGGACT CTAATAGCAA CCAACAGGAC
    CCAGGCGAGC CTGGGGAAAC AGAAGTGCAG GAGGAGGATC ATGATGTCAC TCAGACAGAG
    GTGGCAGATG GGGATATCAT GGATGGGGAG GCTGAAACCG ACTCAGTGGT GATTGCTGGG
    CAGCCTGAGG TGCTCAGTTC ACAAGAGATG CAGGTTGAGA ATGAGCTGGA GGACCTGATA
    GATGAGTTGC TTGAGAGGGA TGGCGGATCT GGGAACAGTA CAATTATAGT GAGCAGAAGT
    GGAGAGGATG AATCACAAGA GGACGTGCTG ATGGATGAAG CTCCTTCCAA CCTCAGCCAA
    GCTTCCACCT TGCAGGCCAA CCGAGAAGAT TCCATGAATA TCCTGGACCC TGAGGATGAG
    GAGGAGCACA CTCAGGAAGA GGACAGCAGT GGCAGTAACG AGGATGAGGA TGATAGTCAG
    GATGAAGAGG AGGAGGAGGA GGAAGATGAG GAAGATGATC AGGAGGATGA TGAAGGTGAA
    GAGGGAGATG AAGACGATGA CGACGATGGC TCTGAAATGG AATTGGATGA GGATTACCCT
    GATATGAACG CTTCTCCCTT GGTCCGATTT GAGCGCTTTG ACCGGGAGGA TGATCTCATC
    ATTGAGTTTG ACAACATGTT CTCCAGTGCT ACAGACATCC CCCCATCCCC AGGAAATATC
    CCTACCACCC ACCCACTGAT GGTACGCCAT GCAGATCACA GTTCCCTGAC ACTGGGCAGT
    GGCTCCTCTA CAACACGTCT CACCCAGGGC ATTGGGCGCA GTCAAAGGAC CCTGAGACAG
    CTGACAGCCA ACACAGGCCA TACCATTCAC GTTCACTACC CTGGGAATCG CCAGCCCAAT
    CCTCCTCTTA TACTCCAGAG GTTGCTTGGT CCCTCTGCTG CTGCTGACAT CCTTCAGCTG
    AGCAGCAGCC TTCCCCTACA AAGCCGAGGC CGAGCCCGCC TCCTAGTGGG CAACGATGAC
    GTCCACATCA TCGCCCGGTC TGATGATGAA CTACTGGATG ACTTTTTCCA TGATCAGAGC
    ACAGCTACCA GCCAAGCAGG GACCCTATCC AGTATCCCTA CAGCCCTGAC TCGCTGGACA
    GAGGAATGCA AAGTTCTTGA TGCTGAGAGC ATGCATGACT GTGTTTCAGT TGTTAAAGTG
    CCCATTGTCA ATCATCTGGA ATTCCTGAGG GATGAGGAGC TGGAAGAAAG GAGGGAGAAA
    AGGAGGAAAC AACTAGCTGA GGAAGAAACA AAGATAACCG ACAAAGGCAA AGAAGATAAG
    GAGAACAGGG ATCAGAGTGC ACAGTGTGCT GCATCTAAGA CAAATGACTC CACTGAACAG
    AACCTCTCAG ATGGGACTCC TATGCCTGAC AGCTACCCAG CAACCCCATC TTCAACTGAT
    GTAGCTACAT CTGAGCCCAG GGAGACCCTT GTCACTCTGC AGCCCTCCCA ACAGCAACCA
    ACACTCCCAA CCCCACCAGC CTTGGGAGAG ATTCCTCAAG AGCTGCAGTC CCCAATTGGA
    GAAGGTGGGA GCTCTACACA GCTATTGATG CCTGTAGAGC CAGAGGAATT GGGTCCCACA
    AGGCCAAGTG GAGAAGCAGA AACAACTCAG ATGGAGCTAT CTCCAGCTCC CACTATAACC
    TCTCTTTCCC CAGAGAGAGC TGAAGATTCT GATGCTCTGA CAGCTGTCAG CAGTCAACTG
    GAAGGCTCTC CCATGGATAC TAGCAGCCTG GCTTCCTGTA CCCTGGAGGA GGCTGTGGGT
    GACGCCCCAG CAACTGGCAG TTCTGAGCAG CCCACAGCAG GCAGCTCCAC TCCTGGGGAT
    GCCCCACCAG TTGTGACAGA AGTACAAGGC AGGGGTGATG GGTCAGGGGA ACCTACCCAG
    CCCCCTGAGG ACAGCTCTCC CCCTGCATCC TCTGAGAGTT CTTCCACCAG AGATTCCGCG
    GTGGCCATTT CTGGAGCAGA TTCCCGGGGA ATCCTGGAAG AGCCATTGCC TTCAACAAGC
    AGTGAAGAAG AAGATCCTCT TGCAGGTATT AGTCTCCCCG AAGGTGTGGA CCCCTCTTTT
    CTGGCCGCTC TGCCTGATGA CATTCGCCGA GAAGTCCTAC AGAACCAGCT GGGCATTCGT
    CCACCAACAC GAACTGCCCC TTCAACAAAT AACTCAGCTC CTGCAGTGGT GGGGAATCCT
    GGTGTCACCG AAGTGAGCCC TGAGTTCCTG GCTGCTCTGC CTCCAGCCAT CCAGGAGGAA
    GTACTGGCAC AGCAGAGAGC TGAGCAACAG CGACGGGAAC TAGCACAAAA TGCCAGCTCA
    GACACCCCCA TGGACCCTGT GACCTTCATC CAGACTCTGC CCTCAGACCT GCGCCGTAGT
    GTCCTAGAGG ACATGGAGGA CAGTGTGTTG GCCGTGATGC CACCTGACAT CGCAGCTGAA
    GCTCAAGCCC TGAGGCGAGA GCAAGAAGCC CGGCAGAGGC AGCTCATGCA TGAGCGACTT
    TTTGGGCACA GCAGCACCTC TGCACTCTCT GCCATTCTCC GAAGCCCAGC CTTCACCAGT
    CGCCTGAGTG GCAACCGTGG GGTCCAATAC ACTCGCCTTG CTGTGCAAAG AGGTGGCACC
    TTCCAGATGG GGGGTAGCAG CAGCCATAAT AGGCCTTCTG GCAGTAATGT GGACACCCTC
    CTACGTCTTC GAGGACGGCT CCTCCTGGAT CACGAAGCCC TGTCTTGTCT CCTGGTCCTA
    CTTTTTGTGG ATGAGCCAAA ACTCAATACT AGCCGTCTTC ACCGAGTACT GAGAAATCTC
    TGCTACCATG CCCAGACCCG CCACTGGGTT ATCCGTAGCC TGCTTTCCAT CTTGCAGCGA
    AGCAGTGAGA GTGAGCTTTG CATTGAAACA CCCAAGCTCT CCTCAAGTGA GGAAAAGGGC
    AAAAAGTCAA GCAAGAGCTG TGGGTCAAGC AGCCATGAGA ACCGGCCCCT GGACCTGCTA
    CACAAGATGG AGTCTAAGAG TTCCAACCAG CTTTCCTGGC TCTCAGTATC CATGGATGCA
    GCCCTAGGCT GCAGGACTAA TATATTCCAG ATCCAGCGTT CAGGGGGTCG CAAACATACT
    GAGAAACATG CGAGTAGTGG CTCCACTGTC CATATCCACC CCCAGGCTGC TCCTGTTGTC
    TGCAGACATG TTCTGGATAC ACTCATTCAA TTGGCCAAGG TGTTTCCCAG TCACTTCACA
    CAGCAGCGGA CCAAAGAAAC AAACTGTGAG AGTGATCGGG AGAGGGGCAG TAAGGCCTGC
    AGCCCATGCT CTTCACAGTC CACAAGCAGT GGCATTTGCA CAGACTTCTG GGACTTATTG
    GTAAAACTGG ACAACATGAA TGTCAGCCGG AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA
    GTGAGCGCTG GTGGTGAGGG GGAAACCTCC CCATACAGCC TCGAAGCTTC TCCACTGGGG
    CAACTCATGA ACATGTTGTC ACACCCAGTC ATCCGCCGGA GCTCTCTCTT AACTGAGAAG
    CTCCTCAGAC TTCTTTCTCT CATCTCAATT GCTCTCCCAG AAAACAAAGT GTCGGAAGCA
    CAGGCTAATT CTGGCAGCAG TGCTTCCTCC ACCACTGTTG CCACTTCAAC CACATCTACC
    ACCACTATCA GTGCCACCTC CAGCACATCT ACTCTGCCTG CTGCAACCAC CCCTGTCACT
    TCTGTTCCAG CCCTGGTTGC TGCCACGGCT ATATCCACCA TTGCCGTAGC CGCTTCAACC
    ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTGCTACTAC TACTAAGGCC
    AGCAAATCTC CAGCTAAAGT TGGTGATATC GGCAGCAGCA GCAGCACAGA CTTTAAGATG
    GTATCCTCTG GCCTCACTGA AAATCAGCTC CAGCTCTCTG TGGAAGTGTT GACATCCCAT
    TCTTGTTCTG AAGAAGGCCT AGAAGATGCA GCAAATGTGT TACTGCAGCT CTCTCGGGGG
    GACCCTGGGA CCCGGGACAC TGTTTTGAAG TTGCTACTGA ATGGAGCCCG CCATCTGGGT
    TATACGCTTT GTAAACAGAT AGGAACTCTT CTGGCTGAGC TACGGGAATA CAATCTGGAA
    CAGCAGCGGC GAGCCCAGTG TGAAACCCTC TCCCCTGATG GCATGCCTGA GGAACAGCCA
    CAGACCACCA AGCTGAAAGG CAAAATGCAG AGCAGGTTTG ACGTGGCTGA GAATGTGGTA
    ATCGTGGCAT CTCAGAAGCG GCCTCTGGGT GGCCGGGAGC TCCAGCTGCC TTCTATGTCC
    ATGTTGACAT CCAAGACATC TACCCAGAAG TTCTTCTTGA GGGTACTGCA GGTCATCATC
    CAGCTCCGGG ATGACACGCG CCGTGCTAAC AAGAAGGCCA AGCAGACAGG CAGGCTAGGT
    TCCTCCGGTT TAGGCTCAGC TAGCAGCATC CAGGCAGCTG TTCGGCAGCT GGAGGCTGAG
    GCTGATGCCA TTATACAAAT GGTACGTGAG GGTCAGAGGG CGCGGAGACA GCAACAAGCA
    GCAACGTCGG AGTCAAGCCA GTCAGAGGCA TCTGTCCGAA GGGAGGAGTC ACCCATGGAT
    GTGGACCAGC CATCTCCCAG TGCTCAAGAT ACTCAGTCCA TTGGCTCAGA TGGAACTCCA
    CAGGGAGAGA AGGAGAAGGA GGAGAGGCCA CCTGAGTTAC CCCTGCTCAG TGAGCAGCTG
    AACTTGGATG AGCTGTGGGA CATGCTGGGA GAGTGTCTAA AGGAACTGGA GGAATCCCAT
    GACCAGCATG CGGTGCTAGT GCTACAACCT GCTGTCGAGG CCTTCTTTCT GGTCCATGCA
    ACAGAGCGGG AGAGCAAGCC TCCTGTCCGG GACACCCGTG AGAGCCAGCT GGCACACATC
    AAGGACGAGC CACCTCCACT CTCCCCTGCC CCTTTAACCC CAGCCACTCC TTCCTCCCTT
    GACCCGTTCT TCTCCCGGGA GCCCTCATCT ATGCACATCT CCTCAAGCCT GCCCCCTGAC
    ACACAGAAGT TTCTTCGTTT TGCAGAGACT CATCGCACTG TGTTAAACCA GATCCTACGG
    CAGTCCACCA CCCACCTCGC TGATGGGCCT TTTGCTGTCC TGGTAGACTA CATTCGTGTC
    CTCGACTTCG ATGTCAAGCG CAAATATTTC CGCCAAGAGC TGGAGCGTTT GGACGAGGGA
    CTCCGGAAAG AAGACATGGC TGTGCACGTT CGTCGTGATC ATGTCTTTGA AGACTCCTAT
    CGTGAGCTGC ACCGGAAATC CCCTGAAGAA ATGAAGAATC GATTGTATAT AGTATTTGAA
    GGAGAAGAAG GGCAGGATGC TGGAGGGCTC CTGCGGGAGT GGTATATGAT CATCTCTCGA
    GAGATGTTTA ACCCTATGTA TGCCTTGTTC CGTACCTCAC CTGGTGATCG GGTCACATAC
    ACCATCAATC CATCTTCTCA CTGCAACCCC AACCACCTCA GCTACTTCAA GTTTGTTGGA
    CGCATCGTGG CCAAAGCTGT GTATGACAAC CGCCTCCTGG AGTGCTACTT TACTAGGTCC
    TTCTACAAAC ACATTTTGGG CAAGTCTGTC AGATATACAG ATATGGAGAG TGAAGACTAC
    CACTTTTACC AGGGCCTGGT TTATCTGCTG GAAAATGATG TCTCCACACT AGGCTATGAT
    CTCACTTTCA GCACTGAGGT CCAAGAGTTT GGAGTCTGTG AAGTTCGTGA CCTCAAACCC
    AATGGGGCCA ACATCTTGGT AACAGAGGAA AATAAGAAGG AATATGTACA CCTGGTGTGC
    CAGATGAGAA TGACAGGAGC CATCCGCAAA CAGCTGGCGG CTTTCTTAGA AGGTTTCTAT
    GAGATCATTC CAAAGCGCCT CATTTCCATC TTCACTGAGC AGGAGTTAGA GCTGCTCATA
    TCAGGACTGC CCACAATCGA CATTGACGAT TTGAAATCTA ACACTGAATA TCACAAGTAC
    CAGTCCAACT CTATTCAGAT CCAGTGGTTC TGGAGAGCAC TGCGTTCTTT CGATCAAGCT
    GACCGTGCCA AGTTCCTCCA GTTTGTCACA GGTACTTCCA AGGTGCCCCT GCAAGGTTTT
    GCTGCCCTTG AAGGCATGAA TGGCATTCAG AAGTTTCAAA TCCATCGAGA TGACAGGTCC
    ACAGACCGCC TGCCTTCAGC TCACACATGT TTTAATCAGC TGGACCTGCC TGCCTATGAG
    AGCTTTGAGA AGCTCCGCCA CATGCTACTA TTGGCCATCC AGGAGTGCTC TGAAGGCTTT
    GGGCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843660.1
    CDS442..13713
    Translation

    Target ORF information:

    RefSeq Version XM_024987892.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987892.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GACAAATCTG ATCCTAAACC AGGGGGTATG
    ACTCAAGAGG TTGGCCAGCT CCTGCAAGAC ATGGGTGATG ACGTATACCA GCAGTACCGG
    TCACTTACCC GTCAGAGCAG TGACTTTGAT GCACAGACAG GATTTTCCAT TAATAGTCAG
    GTCTTTGCTG CAGATGGTTC CTCCACTGAG ACTTCCGCAT CTGGGGTATC CCAAGGAGAA
    GTGTCAACTC CGGAGGAGTC TCGGGATGGG AAGAAAGATA AAGAAGGGGA CCGGGCCTCT
    GAGGAAGGCA AGCAGAAAGG CAAGGGCAGC AAACCTTTAA TGCCCACCTC CACTATTCTT
    CGGCTTCTGG CAGAGTTGGT GAGATCCTAT GTTGGTATTG CTACCCTGAT TGCCAACTAC
    AGCTACACTG TGGGCCAGTC TGAGCTGATC AAAGAGGACT GCAGTGTGCT AGCTTTTGTC
    TTGGACCACC TCCTCCCGCA TACCCAGAAT GCAGAAGACA AAGACACACC GGCGCTGGCC
    CGCCTGTTCC TCGCAAGCCT GGCTGCCTCA GGGAGTGGCA CAGATGCCCA GGTGGCCCTT
    GTGAATGAAG TAAAAGCAGC CCTGGGAAGG GCACTGGCTA TGGCTGAGAG TACAGAGAAA
    CATGCCAGAC TTCAGGCAGT GATGTGTATC ATCAGTACTA TCATGGAGTC CTGCCCCTCC
    ACCTCCAGTT TCTACAGCAG TGCAACAGCC AAGACCCAAC ACAACGGCAT GAACAACATC
    ATTCGACTCT TCCTGAAGAA GGGACTGGTT AATGACCTGG CCAGAGTGCC TCACAGCCTG
    GACCTGTCCA GTCCCAACAT GGCCAACACT GTCAATGCTG CTCTGAAGCC TTTGGAAACA
    CTTTCCCGGA TTGTGAACCA GCCCAGTAGC CTTTTTGGCA GTAAGAGTGC TTCTAGCAAG
    AGCAAGTCTG AGCAGGATGC CCAAGGAGCT GCTCAGGACT CTAATAGCAA CCAACAGGAC
    CCAGGCGAGC CTGGGGAAAC AGAAGTGCAG GAGGAGGATC ATGATGTCAC TCAGACAGAG
    GTGGCAGATG GGGATATCAT GGATGGGGAG GCTGAAACCG ACTCAGTGGT GATTGCTGGG
    CAGCCTGAGG TGCTCAGTTC ACAAGAGATG CAGGTTGAGA ATGAGCTGGA GGACCTGATA
    GATGAGTTGC TTGAGAGGGA TGGCGGATCT GGGAACAGTA CAATTATAGT GAGCAGAAGT
    GGAGAGGATG AATCACAAGA GGACGTGCTG ATGGATGAAG CTCCTTCCAA CCTCAGCCAA
    GCTTCCACCT TGCAGGCCAA CCGAGAAGAT TCCATGAATA TCCTGGACCC TGAGGATGAG
    GAGGAGCACA CTCAGGAAGA GGACAGCAGT GGCAGTAACG AGGATGAGGA TGATAGTCAG
    GATGAAGAGG AGGAGGAGGA GGAAGATGAG GAAGATGATC AGGAGGATGA TGAAGGTGAA
    GAGGGAGATG AAGACGATGA CGACGATGGC TCTGAAATGG AATTGGATGA GGATTACCCT
    GATATGAACG CTTCTCCCTT GGTCCGATTT GAGCGCTTTG ACCGGGAGGA TGATCTCATC
    ATTGAGTTTG ACAACATGTT CTCCAGTGCT ACAGACATCC CCCCATCCCC AGGAAATATC
    CCTACCACCC ACCCACTGAT GGTACGCCAT GCAGATCACA GTTCCCTGAC ACTGGGCAGT
    GGCTCCTCTA CAACACGTCT CACCCAGGGC ATTGGGCGCA GTCAAAGGAC CCTGAGACAG
    CTGACAGCCA ACACAGGCCA TACCATTCAC GTTCACTACC CTGGGAATCG CCAGCCCAAT
    CCTCCTCTTA TACTCCAGAG GTTGCTTGGT CCCTCTGCTG CTGCTGACAT CCTTCAGCTG
    AGCAGCAGCC TTCCCCTACA AAGCCGAGGC CGAGCCCGCC TCCTAGTGGG CAACGATGAC
    GTCCACATCA TCGCCCGGTC TGATGATGAA CTACTGGATG ACTTTTTCCA TGATCAGAGC
    ACAGCTACCA GCCAAGCAGG GACCCTATCC AGTATCCCTA CAGCCCTGAC TCGCTGGACA
    GAGGAATGCA AAGTTCTTGA TGCTGAGAGC ATGCATGACT GTGTTTCAGT TGTTAAAGTG
    CCCATTGTCA ATCATCTGGA ATTCCTGAGG GATGAGGAGC TGGAAGAAAG GAGGGAGAAA
    AGGAGGAAAC AACTAGCTGA GGAAGAAACA AAGATAACCG ACAAAGGCAA AGAAGATAAG
    GAGAACAGGG ATCAGAGTGC ACAGTGTGCT GCATCTAAGA CAAATGACTC CACTGAACAG
    AACCTCTCAG ATGGGACTCC TATGCCTGAC AGCTACCCAG CAACCCCATC TTCAACTGAT
    GTAGCTACAT CTGAGCCCAG GGAGACCCTT GTCACTCTGC AGCCCTCCCA ACAGCAACCA
    ACACTCCCAA CCCCACCAGC CTTGGGAGAG ATTCCTCAAG AGCTGCAGTC CCCAATTGGA
    GAAGGTGGGA GCTCTACACA GCTATTGATG CCTGTAGAGC CAGAGGAATT GGGTCCCACA
    AGGCCAAGTG GAGAAGCAGA AACAACTCAG ATGGAGCTAT CTCCAGCTCC CACTATAACC
    TCTCTTTCCC CAGAGAGAGC TGAAGATTCT GATGCTCTGA CAGCTGTCAG CAGTCAACTG
    GAAGGCTCTC CCATGGATAC TAGCAGCCTG GCTTCCTGTA CCCTGGAGGA GGCTGTGGGT
    GACGCCCCAG CAACTGGCAG TTCTGAGCAG CCCACAGCAG GCAGCTCCAC TCCTGGGGAT
    GCCCCACCAG TTGTGACAGA AGTACAAGGC AGGGGTGATG GGTCAGGGGA ACCTACCCAG
    CCCCCTGAGG ACAGCTCTCC CCCTGCATCC TCTGAGAGTT CTTCCACCAG AGATTCCGCG
    GTGGCCATTT CTGGAGCAGA TTCCCGGGGA ATCCTGGAAG AGCCATTGCC TTCAACAAGC
    AGTGAAGAAG AAGATCCTCT TGCAGGTATT AGTCTCCCCG AAGGTGTGGA CCCCTCTTTT
    CTGGCCGCTC TGCCTGATGA CATTCGCCGA GAAGTCCTAC AGAACCAGCT GGGCATTCGT
    CCACCAACAC GAACTGCCCC TTCAACAAAT AACTCAGCTC CTGCAGTGGT GGGGAATCCT
    GGTGTCACCG AAGTGAGCCC TGAGTTCCTG GCTGCTCTGC CTCCAGCCAT CCAGGAGGAA
    GTACTGGCAC AGCAGAGAGC TGAGCAACAG CGACGGGAAC TAGCACAAAA TGCCAGCTCA
    GACACCCCCA TGGACCCTGT GACCTTCATC CAGACTCTGC CCTCAGACCT GCGCCGTAGT
    GTCCTAGAGG ACATGGAGGA CAGTGTGTTG GCCGTGATGC CACCTGACAT CGCAGCTGAA
    GCTCAAGCCC TGAGGCGAGA GCAAGAAGCC CGGCAGAGGC AGCTCATGCA TGAGCGACTT
    TTTGGGCACA GCAGCACCTC TGCACTCTCT GCCATTCTCC GAAGCCCAGC CTTCACCAGT
    CGCCTGAGTG GCAACCGTGG GGTCCAATAC ACTCGCCTTG CTGTGCAAAG AGGTGGCACC
    TTCCAGATGG GGGGTAGCAG CAGCCATAAT AGGCCTTCTG GCAGTAATGT GGACACCCTC
    CTACGTCTTC GAGGACGGCT CCTCCTGGAT CACGAAGCCC TGTCTTGTCT CCTGGTCCTA
    CTTTTTGTGG ATGAGCCAAA ACTCAATACT AGCCGTCTTC ACCGAGTACT GAGAAATCTC
    TGCTACCATG CCCAGACCCG CCACTGGGTT ATCCGTAGCC TGCTTTCCAT CTTGCAGCGA
    AGCAGTGAGA GTGAGCTTTG CATTGAAACA CCCAAGCTCT CCTCAAGTGA GGAAAAGGGC
    AAAAAGTCAA GCAAGAGCTG TGGGTCAAGC AGCCATGAGA ACCGGCCCCT GGACCTGCTA
    CACAAGATGG AGTCTAAGAG TTCCAACCAG CTTTCCTGGC TCTCAGTATC CATGGATGCA
    GCCCTAGGCT GCAGGACTAA TATATTCCAG ATCCAGCGTT CAGGGGGTCG CAAACATACT
    GAGAAACATG CGAGTAGTGG CTCCACTGTC CATATCCACC CCCAGGCTGC TCCTGTTGTC
    TGCAGACATG TTCTGGATAC ACTCATTCAA TTGGCCAAGG TGTTTCCCAG TCACTTCACA
    CAGCAGCGGA CCAAAGAAAC AAACTGTGAG AGTGATCGGG AGAGGGGCAG TAAGGCCTGC
    AGCCCATGCT CTTCACAGTC CACAAGCAGT GGCATTTGCA CAGACTTCTG GGACTTATTG
    GTAAAACTGG ACAACATGAA TGTCAGCCGG AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA
    GTGAGCGCTG GTGGTGAGGG GGAAACCTCC CCATACAGCC TCGAAGCTTC TCCACTGGGG
    CAACTCATGA ACATGTTGTC ACACCCAGTC ATCCGCCGGA GCTCTCTCTT AACTGAGAAG
    CTCCTCAGAC TTCTTTCTCT CATCTCAATT GCTCTCCCAG AAAACAAAGT GTCGGAAGCA
    CAGGCTAATT CTGGCAGCAG TGCTTCCTCC ACCACTGTTG CCACTTCAAC CACATCTACC
    ACCACTATCA GTGCCACCTC CAGCACATCT ACTCTGCCTG CTGCAACCAC CCCTGTCACT
    TCTGTTCCAG CCCTGGTTGC TGCCACGGCT ATATCCACCA TTGCCGTAGC CGCTTCAACC
    ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTGCTACTAC TACTAAGGCC
    AGCAAATCTC CAGCTAAAGT TGGTGATATC GGCAGCAGCA GCAGCACAGA CTTTAAGATG
    GTATCCTCTG GCCTCACTGA AAATCAGCTC CAGCTCTCTG TGGAAGTGTT GACATCCCAT
    TCTTGTTCTG AAGAAGGCCT AGAAGATGCA GCAAATGTGT TACTGCAGCT CTCTCGGGGG
    GACCCTGGGA CCCGGGACAC TGTTTTGAAG TTGCTACTGA ATGGAGCCCG CCATCTGGGT
    TATACGCTTT GTAAACAGAT AGGAACTCTT CTGGCTGAGC TACGGGAATA CAATCTGGAA
    CAGCAGCGGC GAGCCCAGTG TGAAACCCTC TCCCCTGATG GCATGCCTGA GGAACAGCCA
    CAGACCACCA AGCTGAAAGG CAAAATGCAG AGCAGGTTTG ACGTGGCTGA GAATGTGGTA
    ATCGTGGCAT CTCAGAAGCG GCCTCTGGGT GGCCGGGAGC TCCAGCTGCC TTCTATGTCC
    ATGTTGACAT CCAAGACATC TACCCAGAAG TTCTTCTTGA GGGTACTGCA GGTCATCATC
    CAGCTCCGGG ATGACACGCG CCGTGCTAAC AAGAAGGCCA AGCAGACAGG CAGGCTAGGT
    TCCTCCGGTT TAGGCTCAGC TAGCAGCATC CAGGCAGCTG TTCGGCAGCT GGAGGCTGAG
    GCTGATGCCA TTATACAAAT GGTACGTGAG GGTCAGAGGG CGCGGAGACA GCAACAAGCA
    GCAACGTCGG AGTCAAGCCA GTCAGAGGCA TCTGTCCGAA GGGAGGAGTC ACCCATGGAT
    GTGGACCAGC CATCTCCCAG TGCTCAAGAT ACTCAGTCCA TTGGCTCAGA TGGAACTCCA
    CAGGGAGAGA AGGAGAAGGA GGAGAGGCCA CCTGAGTTAC CCCTGCTCAG TGAGCAGCTG
    AACTTGGATG AGCTGTGGGA CATGCTGGGA GAGTGTCTAA AGGAACTGGA GGAATCCCAT
    GACCAGCATG CGGTGCTAGT GCTACAACCT GCTGTCGAGG CCTTCTTTCT GGTCCATGCA
    ACAGAGCGGG AGAGCAAGCC TCCTGTCCGG GACACCCGTG AGAGCCAGCT GGCACACATC
    AAGGACGAGC CACCTCCACT CTCCCCTGCC CCTTTAACCC CAGCCACTCC TTCCTCCCTT
    GACCCGTTCT TCTCCCGGGA GCCCTCATCT ATGCACATCT CCTCAAGCCT GCCCCCTGAC
    ACACAGAAGT TTCTTCGTTT TGCAGAGACT CATCGCACTG TGTTAAACCA GATCCTACGG
    CAGTCCACCA CCCACCTCGC TGATGGGCCT TTTGCTGTCC TGGTAGACTA CATTCGTGTC
    CTCGACTTCG ATGTCAAGCG CAAATATTTC CGCCAAGAGC TGGAGCGTTT GGACGAGGGA
    CTCCGGAAAG AAGACATGGC TGTGCACGTT CGTCGTGATC ATGTCTTTGA AGACTCCTAT
    CGTGAGCTGC ACCGGAAATC CCCTGAAGAA ATGAAGAATC GATTGTATAT AGTATTTGAA
    GGAGAAGAAG GGCAGGATGC TGGAGGGCTC CTGCGGGAGT GGTATATGAT CATCTCTCGA
    GAGATGTTTA ACCCTATGTA TGCCTTGTTC CGTACCTCAC CTGGTGATCG GGTCACATAC
    ACCATCAATC CATCTTCTCA CTGCAACCCC AACCACCTCA GCTACTTCAA GTTTGTTGGA
    CGCATCGTGG CCAAAGCTGT GTATGACAAC CGCCTCCTGG AGTGCTACTT TACTAGGTCC
    TTCTACAAAC ACATTTTGGG CAAGTCTGTC AGATATACAG ATATGGAGAG TGAAGACTAC
    CACTTTTACC AGGGCCTGGT TTATCTGCTG GAAAATGATG TCTCCACACT AGGCTATGAT
    CTCACTTTCA GCACTGAGGT CCAAGAGTTT GGAGTCTGTG AAGTTCGTGA CCTCAAACCC
    AATGGGGCCA ACATCTTGGT AACAGAGGAA AATAAGAAGG AATATGTACA CCTGGTGTGC
    CAGATGAGAA TGACAGGAGC CATCCGCAAA CAGCTGGCGG CTTTCTTAGA AGGTTTCTAT
    GAGATCATTC CAAAGCGCCT CATTTCCATC TTCACTGAGC AGGAGTTAGA GCTGCTCATA
    TCAGGACTGC CCACAATCGA CATTGACGAT TTGAAATCTA ACACTGAATA TCACAAGTAC
    CAGTCCAACT CTATTCAGAT CCAGTGGTTC TGGAGAGCAC TGCGTTCTTT CGATCAAGCT
    GACCGTGCCA AGTTCCTCCA GTTTGTCACA GGTACTTCCA AGGTGCCCCT GCAAGGTTTT
    GCTGCCCTTG AAGGCATGAA TGGCATTCAG AAGTTTCAAA TCCATCGAGA TGACAGGTCC
    ACAGACCGCC TGCCTTCAGC TCACACATGT TTTAATCAGC TGGACCTGCC TGCCTATGAG
    AGCTTTGAGA AGCTCCGCCA CATGCTACTA TTGGCCATCC AGGAGTGCTC TGAAGGCTTT
    GGGCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238332
    Clone ID Related Accession (Same CDS sequence) XM_024987900.1
    Accession Version XM_024987900.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
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    2521
    2581
    2641
    2701
    2761
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    3001
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    3181
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    4021
    4081
    4141
    4201
    4261
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    4381
    4441
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    4681
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    4981
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    5521
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    5641
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    5881
    5941
    6001
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    6121
    6181
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    6301
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    6421
    6481
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    7141
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    9061
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    9181
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    9301
    9361
    9421
    9481
    9541
    9601
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    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGAA CGGGAAGCCT
    GCTGAGCAAA TGGTACCCTG GGTTGAGGGA GACCACACTT GCAAGACCCC ACCAAATTGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843668.1
    CDS447..12809
    Translation

    Target ORF information:

    RefSeq Version XM_024987900.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987900.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGAA CGGGAAGCCT
    GCTGAGCAAA TGGTACCCTG GGTTGAGGGA GACCACACTT GCAAGACCCC ACCAAATTGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35633
    Clone ID Related Accession (Same CDS sequence) XM_024987894.1 , XM_010821997.2 , XM_005228212.2 , XM_005228212.3 , XM_024987895.1
    Accession Version XM_024987894.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
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    1801
    1861
    1921
    1981
    2041
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    12181
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    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATAA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTCCACTT AGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGATTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT
    GTACTTGTGC GAAACTGTAT CCAGGCCATG ATTGACCCTT CCATGGATCC ATACCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CAAGCTACGA TGCTGGTGGC
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG CCGAAAAGAA TGCCCTTTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTGCACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TACAGATGTG
    ACTATGGAAA GTAGTCCCAG CTCATCCATC TCTATGGAGC ACCGGCTGGA TGTTGAATTA
    AGGGCATCAA GTTCCAGCAG CAGTAGCATC TCCTCTGGCC CTGGCCCTCG TCCTGGAGTC
    CAGTGTATTC CACAACGAGC CGCACTTCTC AAATCCATGT TGAATTTCCT CAAGAAGGCC
    ATTCAAGACC CTGCTTTCTC AGATGGCATA CGACATGTGA TGGATGGTTC TCTGCCTACC
    TCCCTGAAAC ACATCATCAG CAATGCAGAA TACTATGGCC CATCACTCTT TCTCCTAGCT
    ACTGAAGTGG TGACTGTGTT TGTATTTCAA GAACCATCAT TGCTTTCCTC ACTCCAGGAC
    AATGGATTGA CAGATGTCAT GCTACATGCA CTGCTTATCA AAGACGTTCC TGCTACCCGT
    GAAGTTCTTG GCTCCCTCCC AAATGTTTTC AGTGCACTCT GCTTGAATGC CCGAGGTCTG
    CAGTCATTTG TACAGTGTCA GCCTTTTGAA CGTCTCTTCA AAGTTCTCCT GTCTCCAGAT
    TACCTCCCAG CCATGCGGAG AAGGAGGAGT TCTGATCCTC TAGGGGATAC TGCCTCCAAC
    CTAGGAAGTG CTGTAGATGA GCTCATGAGA CATCAGCCTA CCCTCAAAAC AGATGCAACG
    ACTGCGATCA TCAAGTTACT TGAAGAAATC TGTAATCTTG GAAGAGACCC TAAATACATC
    TGTCAGAAGC CATCAATCCA GAAGGCAGAT GGCACTGCCG CTGCTCCTCC CCCAAGATCT
    AACCATGCTG CAGAAGAGGC CTCTAGTGAG GATGAGGAGG AAGAGGAAGT ACAGGCCATG
    CAGAGCTTTA ATTCTACCCA GCAGAATGAA ACTGAGCCTA ATCAGCAGGT TGTTGGTACA
    GAGGAGCGTA TTCCTATTCC CCTCATGGAT TACATCCTTA ATGTGATGAA ATTTGTGGAA
    TCTATTCTGA GCAACAATAC AACTGATGAC CACTGCCAGG AATTTGTGAA TCAGAAAGGA
    CTCTTGCCTT TGGTCACCAT TTTGGGTCTT CCCAATCTGC CCATTGACTT TCCCACGTCT
    GCAGCCTGTC AGGCTGTTGC AGGTGTCTGC AAATCCATAT TGACACTGTC ACATGAACCC
    AAAGTCCTCC AGGAAGGTCT CCTTCAGTTG GACTCCATTC TTTCCTCCTT GGAGCCCTTA
    CACCGCCCAA TAGAATCCCC CGGGGGCTCA GTGTTGTTGC GAGAACTGGC TTGTGCTGGC
    AATGTTGCTG ATGCTACCCT CTCAGCTCAA GCCACACCTC TACTACATGC ACTCACTGCT
    GCCCATGCTT ACATCATGAT GTTTGTTCAT ACTTGCAGGG TTGGACAGAG TGAAATTCGT
    TCCATCTCCA TAAACCAGTG GGGCTCTCAG TTGGGTCTGA GTGTTCTGAG CAAGCTGAGC
    CAGTTATACT GTTCCCTGGT ATGGGAAAGC ACTGTCCTCC TCTCCCTCTG TACCCCAAAC
    AGCTTGCCAT CTGGGTGTGA ATTTGGCCAG GCAGACATGC AGAAACTGGT TCCAAAGGAT
    GAGAAGACAG GTACGACCCA GGGCGGAAAA AGATCAGATG GGGATCAGGA TGGAACCACT
    GGAAGTATGG ATGCTTCTAC CCAGGGCTTG TTGGAAGGCA TTGGGCTTGA TGGTGACACA
    CTGGCTCCTA TGGAGACAGA CGAACCTACT GCTTCAGACT CTAAGGGCAA ATCTAAAATC
    ACACCTGCCA TGGCTGCCAG AATCAAGCAA ATCAAGCCAT TGTTGTCAGC TTCCTCTAGA
    TTAGGCAGAG CACTGGCTGA GCTGTTTGGA CTCCTTGTTA AACTTTGTGT GGGGTCTCCT
    GTCCGCCAGA GAAGAAGCCA TCATGCTGCC AGCACCACCA CAGCACCAAC ACCTGCTGCT
    CGATCAACGG CCTCAGCCCT CACTAAGCTC CTCACAAAAG GCTTATCATG GCAGCCTCCA
    CCATATACGC CTACTCCACG CTTCAGGCTG ACCTTCTTCA TCTGTTCAGT TGGTTTTACA
    TCCCCAATGC TGTTTGATGA GAGGAAGTAT CCCTACCACC TCATGCTGCA GAAATTTCTC
    TGCTCTGGAG GCCACAATGC CCTTTTTGAA ACATTCAACT GGGCTCTGTC TATGGGAGGA
    AAAGTTCCTG TTGCTGAGGG ATTAGAACAT TCAGACTTAC CTGATGGCAC AGGAGAATTC
    CTAGATGCCT GGCTAATGCT GGTGGAGAAG ATGGTAAATC CCACCACTGT GCTTGAATCT
    CCACATTCAC TGCCTGCCAA ATTGCCTGGA GGTGTTCAGA ACTTTCCACA GTTCAGTGCC
    CTCCGCTTCC TTGTGGTAAC TCAGAAGGCA GCATTTACTT GCATCAAGAA CCTGTGGAAC
    CGGAAACCCT TGAAAGTGTA TGGTGGGCGA ATGGCTGAGT CCATGCTAGC CATCCTGTGC
    CACATCCTCC GAGGAGAACC TGTGATTCGA GAGAGACTGA GCAAAGAAAA GGAGGGATCT
    CGAGGAGAAG AGGATACAGG ACAAGAAGAA GGTGGCTCTC GCCGGGAACC TCAAGTCAAC
    CAGCAACAAC TACAACAGCT CATGGACATG GGCTTCACAA GGGAACATGC CATGGAGGCC
    CTGTTGAACA CCAGTACCAT GGAGCAGGCC ACAGAGTACC TTCTAACCCA CCCTCCCCCG
    ATCATGGGAG GAGTTGTTCG GGATCTGAGT ATGTCTGAAG AGGACCAGAT GATGAGAGCG
    ATTGCCATGT CTCTGGGACA GGATATCCCA ATGGATCAAA GAGCAGAGTC ACCTGAGGAA
    GTTGCTTGCC GAAAGGAAGA GGAGGAACGG AAAGCTCGAG AAAAGCAGGA AGAGGAAGAG
    GCTAAATGTC TAGAGAAGTT CCAGGATGCT GATCCATTGG AGCAAGATGA GCTCCACACT
    TTCACAGATA CAATGTTGCC AGGCTGCTTT CACCTTCTTG ATGAGTTGCC AGACACTGTA
    TACCGAGTGT GTGATCTGAT CATGACAGCA ATCAAACGAA ATGGAGCAGA CTATCGAGAC
    ATGATTCTGA AGCAAGTAGT TAATCAGGTG TGGGAAGCTG CTGATGTATT GATCAAAGCT
    GCTCTTCCCC TGACAACAAG TGACACAAAA ACTGTATCAG AGTGGATCAG TCAGATGGCC
    ACTCTACCCC AGGCCTCTAA TTTGGCCACT AGAATCTTGC TTTTAACACT GCTTTTTGAG
    GAATTGAAGC TACCTTGTGC TTGGGTGGTT GAATCTAGTG GCATCCTTAA TGTCCTAATC
    AAACTCTTGG AAGTGGTTCA GCCCTGCCTA CAGGCCGCCA AGGAACAAAA GGAGGTCCAG
    ACCCCAAAGT GGATCACTCC AGTGTTGCTC CTGATTGATT TCTATGAAAA AACAGCCATT
    TCCTCAAAGA GGAGAGCCCA AATGACTAAG TATCTACAGT CTAACAACAA CAACTGGCGC
    TGGTTTGATG ACCGCTCTGG ACGTTGGTGT AGTTACAGTG CAAGCAACAA CAGCACTATT
    GATTCTGCCT GGAAATCTGG AGAGACAAGT GTGCGTTTCA CTGCAGGCCG AAGAAGATAC
    ACTGTCCAGT TCACTACAAT GGTACAGGTT AATGAGGAAA CAGGGAACCG GCGTCCTGTG
    ATGCTGACAC TCCTGAGGGT ACCCCGATTG AATAAAAATT CTAAAAACAG CAATGGACAG
    GAACTAGAGA AGACACTGGA AGAAAGCAAA GAAATGGATA TCAAACGTAA AGAAAATAAA
    ACCAGTGATA CCCCCTTGGC CCTAGACAGT ACAAATACTG AAAAGGAGAC AAGCCTGGAA
    GAAACAAAAA TTGGGGAGAT CTTGATCCAG GGCCTGACAG AGGATATGGT GACTGTTTTA
    ATCCGGGCCT GTGTGAGCAT GCTAGGCGTC CCTGTGGATC CAGACACTTT GCATGCCACC
    CTTCGCCTAT GCCTGAGGCT CACCCGAGAC CACAAATATG CCATGATGTT TGCAGAGCTG
    AAGAGTACTC GCATGATCTT AAATTTGACC CAGAGTTCAG GCTTCAATGG GTTTACTCCC
    CTGGTTACCC TTCTCTTAAG ACACATCATT GAGGACCCCT GCACCCTGCG CCATACCATG
    GAAAAGGTTG TTCGCTCAGC AGCTACAAGT GGAGCTGGTA GCACTACTTC TGGTGTTGTA
    TCTGGCAGCC TTGGCTCTCG GGAGATCAAC TACATCCTTC GTGTCCTTGG ACCAGCCGCA
    TGCCGCAACC CAGACATTTT CACAGAAGTG GCCAACTGCT GTATCCGCAT TGCCCTTCCA
    GCCCCTCGCG GCTCAGGAAC TGCTTCAGAT GATGAGTTTG AGAATCTCAG AATTAAAGGC
    CCGAATGCTG TACAGCTGGT GAAAACAACC CCTTTGAAGC CCTCACCTCT GCCTGTCATT
    CCTGATACTA TCAAGGAAGT GATCTATGAC ATGCTAAATG CCCTGGCTGC ATACCATGCT
    CCAGAGGAAG CAGACAAATC TGATCCTAAA CCAGGGGGTA TGACTCAAGA GGTTGGCCAG
    CTCCTGCAAG ACATGGGTGA TGACGTATAC CAGCAGTACC GGTCACTTAC CCGTCAGAGC
    AGTGACTTTG ATGCACAGAC AGGATTTTCC ATTAATAGTC AGGTCTTTGC TGCAGATGGT
    TCCTCCACTG AGACTTCCGC ATCTGGGGTA TCCCAAGGAG AAGTGTCAAC TCCGGAGGAG
    TCTCGGGATG GGAAGAAAGA TAAAGAAGGG GACCGGGCCT CTGAGGAAGG CAAGCAGAAA
    GGCAAGGGCA GCAAACCTTT AATGCCCACC TCCACTATTC TTCGGCTTCT GGCAGAGTTG
    GTGAGATCCT ATGTTGGTAT TGCTACCCTG ATTGCCAACT ACAGCTACAC TGTGGGCCAG
    TCTGAGCTGA TCAAAGAGGA CTGCAGTGTG CTAGCTTTTG TCTTGGACCA CCTCCTCCCG
    CATACCCAGA ATGCAGAAGA CAAAGACACA CCGGCGCTGG CCCGCCTGTT CCTCGCAAGC
    CTGGCTGCCT CAGGGAGTGG CACAGATGCC CAGGTGGCCC TTGTGAATGA AGTAAAAGCA
    GCCCTGGGAA GGGCACTGGC TATGGCTGAG AGTACAGAGA AACATGCCAG ACTTCAGGCA
    GTGATGTGTA TCATCAGTAC TATCATGGAG TCCTGCCCCT CCACCTCCAG TTTCTACAGC
    AGTGCAACAG CCAAGACCCA ACACAACGGC ATGAACAACA TCATTCGACT CTTCCTGAAG
    AAGGGACTGG TTAATGACCT GGCCAGAGTG CCTCACAGCC TGGACCTGTC CAGTCCCAAC
    ATGGCCAACA CTGTCAATGC TGCTCTGAAG CCTTTGGAAA CACTTTCCCG GATTGTGAAC
    CAGCCCAGTA GCCTTTTTGG CAGTAAGAGT GCTTCTAGCA AGAGCAAGTC TGAGCAGGAT
    GCCCAAGGAG CTGCTCAGGA CTCTAATAGC AACCAACAGG ACCCAGGCGA GCCTGGGGAA
    ACAGAAGTGC AGGAGGAGGA TCATGATGTC ACTCAGACAG AGGTGGCAGA TGGGGATATC
    ATGGATGGGG AGGCTGAAAC CGACTCAGTG GTGATTGCTG GGCAGCCTGA GGTGCTCAGT
    TCACAAGAGA TGCAGGTTGA GAATGAGCTG GAGGACCTGA TAGATGAGTT GCTTGAGAGG
    GATGGCGGAT CTGGGAACAG TACAATTATA GTGAGCAGAA GTGGAGAGGA TGAATCACAA
    GAGGACGTGC TGATGGATGA AGCTCCTTCC AACCTCAGCC AAGCTTCCAC CTTGCAGGCC
    AACCGAGAAG ATTCCATGAA TATCCTGGAC CCTGAGGATG AGGAGGAGCA CACTCAGGAA
    GAGGACAGCA GTGGCAGTAA CGAGGATGAG GATGATAGTC AGGATGAAGA GGAGGAGGAG
    GAGGAAGATG AGGAAGATGA TCAGGAGGAT GATGAAGGTG AAGAGGGAGA TGAAGACGAT
    GACGACGATG GCTCTGAAAT GGAATTGGAT GAGGATTACC CTGATATGAA CGCTTCTCCC
    TTGGTCCGAT TTGAGCGCTT TGACCGGGAG GATGATCTCA TCATTGAGTT TGACAACATG
    TTCTCCAGTG CTACAGACAT CCCCCCATCC CCAGGAAATA TCCCTACCAC CCACCCACTG
    ATGGTACGCC ATGCAGATCA CAGTTCCCTG ACACTGGGCA GTGGCTCCTC TACAACACGT
    CTCACCCAGG GCATTGGGCG CAGTCAAAGG ACCCTGAGAC AGCTGACAGC CAACACAGGC
    CATACCATTC ACGTTCACTA CCCTGGGAAT CGCCAGCCCA ATCCTCCTCT TATACTCCAG
    AGGTTGCTTG GTCCCTCTGC TGCTGCTGAC ATCCTTCAGC TGAGCAGCAG CCTTCCCCTA
    CAAAGCCGAG GCCGAGCCCG CCTCCTAGTG GGCAACGATG ACGTCCACAT CATCGCCCGG
    TCTGATGATG AACTACTGGA TGACTTTTTC CATGATCAGA GCACAGCTAC CAGCCAAGCA
    GGGACCCTAT CCAGTATCCC TACAGCCCTG ACTCGCTGGA CAGAGGAATG CAAAGTTCTT
    GATGCTGAGA GCATGCATGA CTGTGTTTCA GTTGTTAAAG TGCCCATTGT CAATCATCTG
    GAATTCCTGA GGGATGAGGA GCTGGAAGAA AGGAGGGAGA AAAGGAGGAA ACAACTAGCT
    GAGGAAGAAA CAAAGATAAC CGACAAAGGC AAAGAAGATA AGGAGAACAG GGATCAGAGT
    GCACAGTGTG CTGCATCTAA GACAAATGAC TCCACTGAAC AGAACCTCTC AGATGGGACT
    CCTATGCCTG ACAGCTACCC AGCAACCCCA TCTTCAACTG ATGTAGCTAC ATCTGAGCCC
    AGGGAGACCC TTGTCACTCT GCAGCCCTCC CAACAGCAAC CAACACTCCC AACCCCACCA
    GCCTTGGGAG AGATTCCTCA AGAGCTGCAG TCCCCAATTG GAGAAGGTGG GAGCTCTACA
    CAGCTATTGA TGCCTGTAGA GCCAGAGGAA TTGGGTCCCA CAAGGCCAAG TGGAGAAGCA
    GAAACAACTC AGATGGAGCT ATCTCCAGCT CCCACTATAA CCTCTCTTTC CCCAGAGAGA
    GCTGAAGATT CTGATGCTCT GACAGCTGTC AGCAGTCAAC TGGAAGGCTC TCCCATGGAT
    ACTAGCAGCC TGGCTTCCTG TACCCTGGAG GAGGCTGTGG GTGACGCCCC AGCAACTGGC
    AGTTCTGAGC AGCCCACAGC AGGCAGCTCC ACTCCTGGGG ATGCCCCACC AGTTGTGACA
    GAAGTACAAG GCAGGGGTGA TGGGTCAGGG GAACCTACCC AGCCCCCTGA GGACAGCTCT
    CCCCCTGCAT CCTCTGAGAG TTCTTCCACC AGAGATTCCG CGGTGGCCAT TTCTGGAGCA
    GATTCCCGGG GAATCCTGGA AGAGCCATTG CCTTCAACAA GCAGTGAAGA AGAAGATCCT
    CTTGCAGGTA TTAGTCTCCC CGAAGGTGTG GACCCCTCTT TTCTGGCCGC TCTGCCTGAT
    GACATTCGCC GAGAAGTCCT ACAGAACCAG CTGGGCATTC GTCCACCAAC ACGAACTGCC
    CCTTCAACAA ATAACTCAGC TCCTGCAGTG GTGGGGAATC CTGGTGTCAC CGAAGTGAGC
    CCTGAGTTCC TGGCTGCTCT GCCTCCAGCC ATCCAGGAGG AAGTACTGGC ACAGCAGAGA
    GCTGAGCAAC AGCGACGGGA ACTAGCACAA AATGCCAGCT CAGACACCCC CATGGACCCT
    GTGACCTTCA TCCAGACTCT GCCCTCAGAC CTGCGCCGTA GTGTCCTAGA GGACATGGAG
    GACAGTGTGT TGGCCGTGAT GCCACCTGAC ATCGCAGCTG AAGCTCAAGC CCTGAGGCGA
    GAGCAAGAAG CCCGGCAGAG GCAGCTCATG CATGAGCGAC TTTTTGGGCA CAGCAGCACC
    TCTGCACTCT CTGCCATTCT CCGAAGCCCA GCCTTCACCA GTCGCCTGAG TGGCAACCGT
    GGGGTCCAAT ACACTCGCCT TGCTGTGCAA AGAGGTGGCA CCTTCCAGAT GGGGGGTAGC
    AGCAGCCATA ATAGGCCTTC TGGCAGTAAT GTGGACACCC TCCTACGTCT TCGAGGACGG
    CTCCTCCTGG ATCACGAAGC CCTGTCTTGT CTCCTGGTCC TACTTTTTGT GGATGAGCCA
    AAACTCAATA CTAGCCGTCT TCACCGAGTA CTGAGAAATC TCTGCTACCA TGCCCAGACC
    CGCCACTGGG TTATCCGTAG CCTGCTTTCC ATCTTGCAGC GAAGCAGTGA GAGTGAGCTT
    TGCATTGAAA CACCCAAGCT CTCCTCAAGT GAGGAAAAGG GCAAAAAGTC AAGCAAGAGC
    TGTGGGTCAA GCAGCCATGA GAACCGGCCC CTGGACCTGC TACACAAGAT GGAGTCTAAG
    AGTTCCAACC AGCTTTCCTG GCTCTCAGTA TCCATGGATG CAGCCCTAGG CTGCAGGACT
    AATATATTCC AGATCCAGCG TTCAGGGGGT CGCAAACATA CTGAGAAACA TGCGAGTAGT
    GGCTCCACTG TCCATATCCA CCCCCAGGCT GCTCCTGTTG TCTGCAGACA TGTTCTGGAT
    ACACTCATTC AATTGGCCAA GGTGTTTCCC AGTCACTTCA CACAGCAGCG GACCAAAGAA
    ACAAACTGTG AGAGTGATCG GGAGAGGGGC AGTAAGGCCT GCAGCCCATG CTCTTCACAG
    TCCACAAGCA GTGGCATTTG CACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG
    AATGTCAGCC GGAAAGGCAA GAACTCCGTG AAGTCAGTGC CAGTGAGCGC TGGTGGTGAG
    GGGGAAACCT CCCCATACAG CCTCGAAGCT TCTCCACTGG GGCAACTCAT GAACATGTTG
    TCACACCCAG TCATCCGCCG GAGCTCTCTC TTAACTGAGA AGCTCCTCAG ACTTCTTTCT
    CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTGTCGGAAG CACAGGCTAA TTCTGGCAGC
    AGTGCTTCCT CCACCACTGT TGCCACTTCA ACCACATCTA CCACCACTAT CAGTGCCACC
    TCCAGCACAT CTACTCTGCC TGCTGCAACC ACCCCTGTCA CTTCTGTTCC AGCCCTGGTT
    GCTGCCACGG CTATATCCAC CATTGCCGTA GCCGCTTCAA CCACAGTGAC TACCCCCACA
    ACTGCTACCA CTACTGTTTC AACTGCTACT ACTACTAAGG CCAGCAAATC TCCAGCTAAA
    GTTGGTGATA TCGGCAGCAG CAGCAGCACA GACTTTAAGA TGGTATCCTC TGGCCTCACT
    GAAAATCAGC TCCAGCTCTC TGTGGAAGTG TTGACATCCC ATTCTTGTTC TGAAGAAGGC
    CTAGAAGATG CAGCAAATGT GTTACTGCAG CTCTCTCGGG GGGACCCTGG GACCCGGGAC
    ACTGTTTTGA AGTTGCTACT GAATGGAGCC CGCCATCTGG GTTATACGCT TTGTAAACAG
    ATAGGAACTC TTCTGGCTGA GCTACGGGAA TACAATCTGG AACAGCAGCG GCGAGCCCAG
    TGTGAAACCC TCTCCCCTGA TGGCATGCCT GAGGAACAGC CACAGACCAC CAAGCTGAAA
    GGCAAAATGC AGAGCAGGTT TGACGTGGCT GAGAATGTGG TAATCGTGGC ATCTCAGAAG
    CGGCCTCTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA
    TCTACCCAGA AGTTCTTCTT GAGGGTACTG CAGGTCATCA TCCAGCTCCG GGATGACACG
    CGCCGTGCTA ACAAGAAGGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA
    GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA
    ATGGTACGTG AGGGTCAGAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCAAGC
    CAGTCAGAGG CATCTGTCCG AAGGGAGGAG TCACCCATGG ATGTGGACCA GCCATCTCCC
    AGTGCTCAAG ATACTCAGTC CATTGGCTCA GATGGAACTC CACAGGGAGA GAAGGAGAAG
    GAGGAGAGGC CACCTGAGTT ACCCCTGCTC AGTGAGCAGC TGAACTTGGA TGAGCTGTGG
    GACATGCTGG GAGAGTGTCT AAAGGAACTG GAGGAATCCC ATGACCAGCA TGCGGTGCTA
    GTGCTACAAC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CAACAGAGCG GGAGAGCAAG
    CCTCCTGTCC GGGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCACCTCCA
    CTCTCCCCTG CCCCTTTAAC CCCAGCCACT CCTTCCTCCC TTGACCCGTT CTTCTCCCGG
    GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTTCTTCGT
    TTTGCAGAGA CTCATCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC CACCCACCTC
    GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT CGATGTCAAG
    CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTGGACGAGG GACTCCGGAA AGAAGACATG
    GCTGTGCACG TTCGTCGTGA TCATGTCTTT GAAGACTCCT ATCGTGAGCT GCACCGGAAA
    TCCCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT
    GCTGGAGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG
    TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGGGTCACAT ACACCATCAA TCCATCTTCT
    CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTTG GACGCATCGT GGCCAAAGCT
    GTGTATGACA ACCGCCTCCT GGAGTGCTAC TTTACTAGGT CCTTCTACAA ACACATTTTG
    GGCAAGTCTG TCAGATATAC AGATATGGAG AGTGAAGACT ACCACTTTTA CCAGGGCCTG
    GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ATCTCACTTT CAGCACTGAG
    GTCCAAGAGT TTGGAGTCTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG
    GTAACAGAGG AAAATAAGAA GGAATATGTA CACCTGGTGT GCCAGATGAG AATGACAGGA
    GCCATCCGCA AACAGCTGGC GGCTTTCTTA GAAGGTTTCT ATGAGATCAT TCCAAAGCGC
    CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTCA TATCAGGACT GCCCACAATC
    GACATTGACG ATTTGAAATC TAACACTGAA TATCACAAGT ACCAGTCCAA CTCTATTCAG
    ATCCAGTGGT TCTGGAGAGC ACTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC
    CAGTTTGTCA CAGGTACTTC CAAGGTGCCC CTGCAAGGTT TTGCTGCCCT TGAAGGCATG
    AATGGCATTC AGAAGTTTCA AATCCATCGA GATGACAGGT CCACAGACCG CCTGCCTTCA
    GCTCACACAT GTTTTAATCA GCTGGACCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC
    CACATGCTAC TATTGGCCAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843662.1
    CDS345..13598
    Translation

    Target ORF information:

    RefSeq Version XM_024987894.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987894.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATAA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTCCACTT AGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGATTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT
    GTACTTGTGC GAAACTGTAT CCAGGCCATG ATTGACCCTT CCATGGATCC ATACCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CAAGCTACGA TGCTGGTGGC
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG CCGAAAAGAA TGCCCTTTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTGCACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TACAGATGTG
    ACTATGGAAA GTAGTCCCAG CTCATCCATC TCTATGGAGC ACCGGCTGGA TGTTGAATTA
    AGGGCATCAA GTTCCAGCAG CAGTAGCATC TCCTCTGGCC CTGGCCCTCG TCCTGGAGTC
    CAGTGTATTC CACAACGAGC CGCACTTCTC AAATCCATGT TGAATTTCCT CAAGAAGGCC
    ATTCAAGACC CTGCTTTCTC AGATGGCATA CGACATGTGA TGGATGGTTC TCTGCCTACC
    TCCCTGAAAC ACATCATCAG CAATGCAGAA TACTATGGCC CATCACTCTT TCTCCTAGCT
    ACTGAAGTGG TGACTGTGTT TGTATTTCAA GAACCATCAT TGCTTTCCTC ACTCCAGGAC
    AATGGATTGA CAGATGTCAT GCTACATGCA CTGCTTATCA AAGACGTTCC TGCTACCCGT
    GAAGTTCTTG GCTCCCTCCC AAATGTTTTC AGTGCACTCT GCTTGAATGC CCGAGGTCTG
    CAGTCATTTG TACAGTGTCA GCCTTTTGAA CGTCTCTTCA AAGTTCTCCT GTCTCCAGAT
    TACCTCCCAG CCATGCGGAG AAGGAGGAGT TCTGATCCTC TAGGGGATAC TGCCTCCAAC
    CTAGGAAGTG CTGTAGATGA GCTCATGAGA CATCAGCCTA CCCTCAAAAC AGATGCAACG
    ACTGCGATCA TCAAGTTACT TGAAGAAATC TGTAATCTTG GAAGAGACCC TAAATACATC
    TGTCAGAAGC CATCAATCCA GAAGGCAGAT GGCACTGCCG CTGCTCCTCC CCCAAGATCT
    AACCATGCTG CAGAAGAGGC CTCTAGTGAG GATGAGGAGG AAGAGGAAGT ACAGGCCATG
    CAGAGCTTTA ATTCTACCCA GCAGAATGAA ACTGAGCCTA ATCAGCAGGT TGTTGGTACA
    GAGGAGCGTA TTCCTATTCC CCTCATGGAT TACATCCTTA ATGTGATGAA ATTTGTGGAA
    TCTATTCTGA GCAACAATAC AACTGATGAC CACTGCCAGG AATTTGTGAA TCAGAAAGGA
    CTCTTGCCTT TGGTCACCAT TTTGGGTCTT CCCAATCTGC CCATTGACTT TCCCACGTCT
    GCAGCCTGTC AGGCTGTTGC AGGTGTCTGC AAATCCATAT TGACACTGTC ACATGAACCC
    AAAGTCCTCC AGGAAGGTCT CCTTCAGTTG GACTCCATTC TTTCCTCCTT GGAGCCCTTA
    CACCGCCCAA TAGAATCCCC CGGGGGCTCA GTGTTGTTGC GAGAACTGGC TTGTGCTGGC
    AATGTTGCTG ATGCTACCCT CTCAGCTCAA GCCACACCTC TACTACATGC ACTCACTGCT
    GCCCATGCTT ACATCATGAT GTTTGTTCAT ACTTGCAGGG TTGGACAGAG TGAAATTCGT
    TCCATCTCCA TAAACCAGTG GGGCTCTCAG TTGGGTCTGA GTGTTCTGAG CAAGCTGAGC
    CAGTTATACT GTTCCCTGGT ATGGGAAAGC ACTGTCCTCC TCTCCCTCTG TACCCCAAAC
    AGCTTGCCAT CTGGGTGTGA ATTTGGCCAG GCAGACATGC AGAAACTGGT TCCAAAGGAT
    GAGAAGACAG GTACGACCCA GGGCGGAAAA AGATCAGATG GGGATCAGGA TGGAACCACT
    GGAAGTATGG ATGCTTCTAC CCAGGGCTTG TTGGAAGGCA TTGGGCTTGA TGGTGACACA
    CTGGCTCCTA TGGAGACAGA CGAACCTACT GCTTCAGACT CTAAGGGCAA ATCTAAAATC
    ACACCTGCCA TGGCTGCCAG AATCAAGCAA ATCAAGCCAT TGTTGTCAGC TTCCTCTAGA
    TTAGGCAGAG CACTGGCTGA GCTGTTTGGA CTCCTTGTTA AACTTTGTGT GGGGTCTCCT
    GTCCGCCAGA GAAGAAGCCA TCATGCTGCC AGCACCACCA CAGCACCAAC ACCTGCTGCT
    CGATCAACGG CCTCAGCCCT CACTAAGCTC CTCACAAAAG GCTTATCATG GCAGCCTCCA
    CCATATACGC CTACTCCACG CTTCAGGCTG ACCTTCTTCA TCTGTTCAGT TGGTTTTACA
    TCCCCAATGC TGTTTGATGA GAGGAAGTAT CCCTACCACC TCATGCTGCA GAAATTTCTC
    TGCTCTGGAG GCCACAATGC CCTTTTTGAA ACATTCAACT GGGCTCTGTC TATGGGAGGA
    AAAGTTCCTG TTGCTGAGGG ATTAGAACAT TCAGACTTAC CTGATGGCAC AGGAGAATTC
    CTAGATGCCT GGCTAATGCT GGTGGAGAAG ATGGTAAATC CCACCACTGT GCTTGAATCT
    CCACATTCAC TGCCTGCCAA ATTGCCTGGA GGTGTTCAGA ACTTTCCACA GTTCAGTGCC
    CTCCGCTTCC TTGTGGTAAC TCAGAAGGCA GCATTTACTT GCATCAAGAA CCTGTGGAAC
    CGGAAACCCT TGAAAGTGTA TGGTGGGCGA ATGGCTGAGT CCATGCTAGC CATCCTGTGC
    CACATCCTCC GAGGAGAACC TGTGATTCGA GAGAGACTGA GCAAAGAAAA GGAGGGATCT
    CGAGGAGAAG AGGATACAGG ACAAGAAGAA GGTGGCTCTC GCCGGGAACC TCAAGTCAAC
    CAGCAACAAC TACAACAGCT CATGGACATG GGCTTCACAA GGGAACATGC CATGGAGGCC
    CTGTTGAACA CCAGTACCAT GGAGCAGGCC ACAGAGTACC TTCTAACCCA CCCTCCCCCG
    ATCATGGGAG GAGTTGTTCG GGATCTGAGT ATGTCTGAAG AGGACCAGAT GATGAGAGCG
    ATTGCCATGT CTCTGGGACA GGATATCCCA ATGGATCAAA GAGCAGAGTC ACCTGAGGAA
    GTTGCTTGCC GAAAGGAAGA GGAGGAACGG AAAGCTCGAG AAAAGCAGGA AGAGGAAGAG
    GCTAAATGTC TAGAGAAGTT CCAGGATGCT GATCCATTGG AGCAAGATGA GCTCCACACT
    TTCACAGATA CAATGTTGCC AGGCTGCTTT CACCTTCTTG ATGAGTTGCC AGACACTGTA
    TACCGAGTGT GTGATCTGAT CATGACAGCA ATCAAACGAA ATGGAGCAGA CTATCGAGAC
    ATGATTCTGA AGCAAGTAGT TAATCAGGTG TGGGAAGCTG CTGATGTATT GATCAAAGCT
    GCTCTTCCCC TGACAACAAG TGACACAAAA ACTGTATCAG AGTGGATCAG TCAGATGGCC
    ACTCTACCCC AGGCCTCTAA TTTGGCCACT AGAATCTTGC TTTTAACACT GCTTTTTGAG
    GAATTGAAGC TACCTTGTGC TTGGGTGGTT GAATCTAGTG GCATCCTTAA TGTCCTAATC
    AAACTCTTGG AAGTGGTTCA GCCCTGCCTA CAGGCCGCCA AGGAACAAAA GGAGGTCCAG
    ACCCCAAAGT GGATCACTCC AGTGTTGCTC CTGATTGATT TCTATGAAAA AACAGCCATT
    TCCTCAAAGA GGAGAGCCCA AATGACTAAG TATCTACAGT CTAACAACAA CAACTGGCGC
    TGGTTTGATG ACCGCTCTGG ACGTTGGTGT AGTTACAGTG CAAGCAACAA CAGCACTATT
    GATTCTGCCT GGAAATCTGG AGAGACAAGT GTGCGTTTCA CTGCAGGCCG AAGAAGATAC
    ACTGTCCAGT TCACTACAAT GGTACAGGTT AATGAGGAAA CAGGGAACCG GCGTCCTGTG
    ATGCTGACAC TCCTGAGGGT ACCCCGATTG AATAAAAATT CTAAAAACAG CAATGGACAG
    GAACTAGAGA AGACACTGGA AGAAAGCAAA GAAATGGATA TCAAACGTAA AGAAAATAAA
    ACCAGTGATA CCCCCTTGGC CCTAGACAGT ACAAATACTG AAAAGGAGAC AAGCCTGGAA
    GAAACAAAAA TTGGGGAGAT CTTGATCCAG GGCCTGACAG AGGATATGGT GACTGTTTTA
    ATCCGGGCCT GTGTGAGCAT GCTAGGCGTC CCTGTGGATC CAGACACTTT GCATGCCACC
    CTTCGCCTAT GCCTGAGGCT CACCCGAGAC CACAAATATG CCATGATGTT TGCAGAGCTG
    AAGAGTACTC GCATGATCTT AAATTTGACC CAGAGTTCAG GCTTCAATGG GTTTACTCCC
    CTGGTTACCC TTCTCTTAAG ACACATCATT GAGGACCCCT GCACCCTGCG CCATACCATG
    GAAAAGGTTG TTCGCTCAGC AGCTACAAGT GGAGCTGGTA GCACTACTTC TGGTGTTGTA
    TCTGGCAGCC TTGGCTCTCG GGAGATCAAC TACATCCTTC GTGTCCTTGG ACCAGCCGCA
    TGCCGCAACC CAGACATTTT CACAGAAGTG GCCAACTGCT GTATCCGCAT TGCCCTTCCA
    GCCCCTCGCG GCTCAGGAAC TGCTTCAGAT GATGAGTTTG AGAATCTCAG AATTAAAGGC
    CCGAATGCTG TACAGCTGGT GAAAACAACC CCTTTGAAGC CCTCACCTCT GCCTGTCATT
    CCTGATACTA TCAAGGAAGT GATCTATGAC ATGCTAAATG CCCTGGCTGC ATACCATGCT
    CCAGAGGAAG CAGACAAATC TGATCCTAAA CCAGGGGGTA TGACTCAAGA GGTTGGCCAG
    CTCCTGCAAG ACATGGGTGA TGACGTATAC CAGCAGTACC GGTCACTTAC CCGTCAGAGC
    AGTGACTTTG ATGCACAGAC AGGATTTTCC ATTAATAGTC AGGTCTTTGC TGCAGATGGT
    TCCTCCACTG AGACTTCCGC ATCTGGGGTA TCCCAAGGAG AAGTGTCAAC TCCGGAGGAG
    TCTCGGGATG GGAAGAAAGA TAAAGAAGGG GACCGGGCCT CTGAGGAAGG CAAGCAGAAA
    GGCAAGGGCA GCAAACCTTT AATGCCCACC TCCACTATTC TTCGGCTTCT GGCAGAGTTG
    GTGAGATCCT ATGTTGGTAT TGCTACCCTG ATTGCCAACT ACAGCTACAC TGTGGGCCAG
    TCTGAGCTGA TCAAAGAGGA CTGCAGTGTG CTAGCTTTTG TCTTGGACCA CCTCCTCCCG
    CATACCCAGA ATGCAGAAGA CAAAGACACA CCGGCGCTGG CCCGCCTGTT CCTCGCAAGC
    CTGGCTGCCT CAGGGAGTGG CACAGATGCC CAGGTGGCCC TTGTGAATGA AGTAAAAGCA
    GCCCTGGGAA GGGCACTGGC TATGGCTGAG AGTACAGAGA AACATGCCAG ACTTCAGGCA
    GTGATGTGTA TCATCAGTAC TATCATGGAG TCCTGCCCCT CCACCTCCAG TTTCTACAGC
    AGTGCAACAG CCAAGACCCA ACACAACGGC ATGAACAACA TCATTCGACT CTTCCTGAAG
    AAGGGACTGG TTAATGACCT GGCCAGAGTG CCTCACAGCC TGGACCTGTC CAGTCCCAAC
    ATGGCCAACA CTGTCAATGC TGCTCTGAAG CCTTTGGAAA CACTTTCCCG GATTGTGAAC
    CAGCCCAGTA GCCTTTTTGG CAGTAAGAGT GCTTCTAGCA AGAGCAAGTC TGAGCAGGAT
    GCCCAAGGAG CTGCTCAGGA CTCTAATAGC AACCAACAGG ACCCAGGCGA GCCTGGGGAA
    ACAGAAGTGC AGGAGGAGGA TCATGATGTC ACTCAGACAG AGGTGGCAGA TGGGGATATC
    ATGGATGGGG AGGCTGAAAC CGACTCAGTG GTGATTGCTG GGCAGCCTGA GGTGCTCAGT
    TCACAAGAGA TGCAGGTTGA GAATGAGCTG GAGGACCTGA TAGATGAGTT GCTTGAGAGG
    GATGGCGGAT CTGGGAACAG TACAATTATA GTGAGCAGAA GTGGAGAGGA TGAATCACAA
    GAGGACGTGC TGATGGATGA AGCTCCTTCC AACCTCAGCC AAGCTTCCAC CTTGCAGGCC
    AACCGAGAAG ATTCCATGAA TATCCTGGAC CCTGAGGATG AGGAGGAGCA CACTCAGGAA
    GAGGACAGCA GTGGCAGTAA CGAGGATGAG GATGATAGTC AGGATGAAGA GGAGGAGGAG
    GAGGAAGATG AGGAAGATGA TCAGGAGGAT GATGAAGGTG AAGAGGGAGA TGAAGACGAT
    GACGACGATG GCTCTGAAAT GGAATTGGAT GAGGATTACC CTGATATGAA CGCTTCTCCC
    TTGGTCCGAT TTGAGCGCTT TGACCGGGAG GATGATCTCA TCATTGAGTT TGACAACATG
    TTCTCCAGTG CTACAGACAT CCCCCCATCC CCAGGAAATA TCCCTACCAC CCACCCACTG
    ATGGTACGCC ATGCAGATCA CAGTTCCCTG ACACTGGGCA GTGGCTCCTC TACAACACGT
    CTCACCCAGG GCATTGGGCG CAGTCAAAGG ACCCTGAGAC AGCTGACAGC CAACACAGGC
    CATACCATTC ACGTTCACTA CCCTGGGAAT CGCCAGCCCA ATCCTCCTCT TATACTCCAG
    AGGTTGCTTG GTCCCTCTGC TGCTGCTGAC ATCCTTCAGC TGAGCAGCAG CCTTCCCCTA
    CAAAGCCGAG GCCGAGCCCG CCTCCTAGTG GGCAACGATG ACGTCCACAT CATCGCCCGG
    TCTGATGATG AACTACTGGA TGACTTTTTC CATGATCAGA GCACAGCTAC CAGCCAAGCA
    GGGACCCTAT CCAGTATCCC TACAGCCCTG ACTCGCTGGA CAGAGGAATG CAAAGTTCTT
    GATGCTGAGA GCATGCATGA CTGTGTTTCA GTTGTTAAAG TGCCCATTGT CAATCATCTG
    GAATTCCTGA GGGATGAGGA GCTGGAAGAA AGGAGGGAGA AAAGGAGGAA ACAACTAGCT
    GAGGAAGAAA CAAAGATAAC CGACAAAGGC AAAGAAGATA AGGAGAACAG GGATCAGAGT
    GCACAGTGTG CTGCATCTAA GACAAATGAC TCCACTGAAC AGAACCTCTC AGATGGGACT
    CCTATGCCTG ACAGCTACCC AGCAACCCCA TCTTCAACTG ATGTAGCTAC ATCTGAGCCC
    AGGGAGACCC TTGTCACTCT GCAGCCCTCC CAACAGCAAC CAACACTCCC AACCCCACCA
    GCCTTGGGAG AGATTCCTCA AGAGCTGCAG TCCCCAATTG GAGAAGGTGG GAGCTCTACA
    CAGCTATTGA TGCCTGTAGA GCCAGAGGAA TTGGGTCCCA CAAGGCCAAG TGGAGAAGCA
    GAAACAACTC AGATGGAGCT ATCTCCAGCT CCCACTATAA CCTCTCTTTC CCCAGAGAGA
    GCTGAAGATT CTGATGCTCT GACAGCTGTC AGCAGTCAAC TGGAAGGCTC TCCCATGGAT
    ACTAGCAGCC TGGCTTCCTG TACCCTGGAG GAGGCTGTGG GTGACGCCCC AGCAACTGGC
    AGTTCTGAGC AGCCCACAGC AGGCAGCTCC ACTCCTGGGG ATGCCCCACC AGTTGTGACA
    GAAGTACAAG GCAGGGGTGA TGGGTCAGGG GAACCTACCC AGCCCCCTGA GGACAGCTCT
    CCCCCTGCAT CCTCTGAGAG TTCTTCCACC AGAGATTCCG CGGTGGCCAT TTCTGGAGCA
    GATTCCCGGG GAATCCTGGA AGAGCCATTG CCTTCAACAA GCAGTGAAGA AGAAGATCCT
    CTTGCAGGTA TTAGTCTCCC CGAAGGTGTG GACCCCTCTT TTCTGGCCGC TCTGCCTGAT
    GACATTCGCC GAGAAGTCCT ACAGAACCAG CTGGGCATTC GTCCACCAAC ACGAACTGCC
    CCTTCAACAA ATAACTCAGC TCCTGCAGTG GTGGGGAATC CTGGTGTCAC CGAAGTGAGC
    CCTGAGTTCC TGGCTGCTCT GCCTCCAGCC ATCCAGGAGG AAGTACTGGC ACAGCAGAGA
    GCTGAGCAAC AGCGACGGGA ACTAGCACAA AATGCCAGCT CAGACACCCC CATGGACCCT
    GTGACCTTCA TCCAGACTCT GCCCTCAGAC CTGCGCCGTA GTGTCCTAGA GGACATGGAG
    GACAGTGTGT TGGCCGTGAT GCCACCTGAC ATCGCAGCTG AAGCTCAAGC CCTGAGGCGA
    GAGCAAGAAG CCCGGCAGAG GCAGCTCATG CATGAGCGAC TTTTTGGGCA CAGCAGCACC
    TCTGCACTCT CTGCCATTCT CCGAAGCCCA GCCTTCACCA GTCGCCTGAG TGGCAACCGT
    GGGGTCCAAT ACACTCGCCT TGCTGTGCAA AGAGGTGGCA CCTTCCAGAT GGGGGGTAGC
    AGCAGCCATA ATAGGCCTTC TGGCAGTAAT GTGGACACCC TCCTACGTCT TCGAGGACGG
    CTCCTCCTGG ATCACGAAGC CCTGTCTTGT CTCCTGGTCC TACTTTTTGT GGATGAGCCA
    AAACTCAATA CTAGCCGTCT TCACCGAGTA CTGAGAAATC TCTGCTACCA TGCCCAGACC
    CGCCACTGGG TTATCCGTAG CCTGCTTTCC ATCTTGCAGC GAAGCAGTGA GAGTGAGCTT
    TGCATTGAAA CACCCAAGCT CTCCTCAAGT GAGGAAAAGG GCAAAAAGTC AAGCAAGAGC
    TGTGGGTCAA GCAGCCATGA GAACCGGCCC CTGGACCTGC TACACAAGAT GGAGTCTAAG
    AGTTCCAACC AGCTTTCCTG GCTCTCAGTA TCCATGGATG CAGCCCTAGG CTGCAGGACT
    AATATATTCC AGATCCAGCG TTCAGGGGGT CGCAAACATA CTGAGAAACA TGCGAGTAGT
    GGCTCCACTG TCCATATCCA CCCCCAGGCT GCTCCTGTTG TCTGCAGACA TGTTCTGGAT
    ACACTCATTC AATTGGCCAA GGTGTTTCCC AGTCACTTCA CACAGCAGCG GACCAAAGAA
    ACAAACTGTG AGAGTGATCG GGAGAGGGGC AGTAAGGCCT GCAGCCCATG CTCTTCACAG
    TCCACAAGCA GTGGCATTTG CACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG
    AATGTCAGCC GGAAAGGCAA GAACTCCGTG AAGTCAGTGC CAGTGAGCGC TGGTGGTGAG
    GGGGAAACCT CCCCATACAG CCTCGAAGCT TCTCCACTGG GGCAACTCAT GAACATGTTG
    TCACACCCAG TCATCCGCCG GAGCTCTCTC TTAACTGAGA AGCTCCTCAG ACTTCTTTCT
    CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTGTCGGAAG CACAGGCTAA TTCTGGCAGC
    AGTGCTTCCT CCACCACTGT TGCCACTTCA ACCACATCTA CCACCACTAT CAGTGCCACC
    TCCAGCACAT CTACTCTGCC TGCTGCAACC ACCCCTGTCA CTTCTGTTCC AGCCCTGGTT
    GCTGCCACGG CTATATCCAC CATTGCCGTA GCCGCTTCAA CCACAGTGAC TACCCCCACA
    ACTGCTACCA CTACTGTTTC AACTGCTACT ACTACTAAGG CCAGCAAATC TCCAGCTAAA
    GTTGGTGATA TCGGCAGCAG CAGCAGCACA GACTTTAAGA TGGTATCCTC TGGCCTCACT
    GAAAATCAGC TCCAGCTCTC TGTGGAAGTG TTGACATCCC ATTCTTGTTC TGAAGAAGGC
    CTAGAAGATG CAGCAAATGT GTTACTGCAG CTCTCTCGGG GGGACCCTGG GACCCGGGAC
    ACTGTTTTGA AGTTGCTACT GAATGGAGCC CGCCATCTGG GTTATACGCT TTGTAAACAG
    ATAGGAACTC TTCTGGCTGA GCTACGGGAA TACAATCTGG AACAGCAGCG GCGAGCCCAG
    TGTGAAACCC TCTCCCCTGA TGGCATGCCT GAGGAACAGC CACAGACCAC CAAGCTGAAA
    GGCAAAATGC AGAGCAGGTT TGACGTGGCT GAGAATGTGG TAATCGTGGC ATCTCAGAAG
    CGGCCTCTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA
    TCTACCCAGA AGTTCTTCTT GAGGGTACTG CAGGTCATCA TCCAGCTCCG GGATGACACG
    CGCCGTGCTA ACAAGAAGGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA
    GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA
    ATGGTACGTG AGGGTCAGAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCAAGC
    CAGTCAGAGG CATCTGTCCG AAGGGAGGAG TCACCCATGG ATGTGGACCA GCCATCTCCC
    AGTGCTCAAG ATACTCAGTC CATTGGCTCA GATGGAACTC CACAGGGAGA GAAGGAGAAG
    GAGGAGAGGC CACCTGAGTT ACCCCTGCTC AGTGAGCAGC TGAACTTGGA TGAGCTGTGG
    GACATGCTGG GAGAGTGTCT AAAGGAACTG GAGGAATCCC ATGACCAGCA TGCGGTGCTA
    GTGCTACAAC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CAACAGAGCG GGAGAGCAAG
    CCTCCTGTCC GGGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCACCTCCA
    CTCTCCCCTG CCCCTTTAAC CCCAGCCACT CCTTCCTCCC TTGACCCGTT CTTCTCCCGG
    GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTTCTTCGT
    TTTGCAGAGA CTCATCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC CACCCACCTC
    GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT CGATGTCAAG
    CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTGGACGAGG GACTCCGGAA AGAAGACATG
    GCTGTGCACG TTCGTCGTGA TCATGTCTTT GAAGACTCCT ATCGTGAGCT GCACCGGAAA
    TCCCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT
    GCTGGAGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG
    TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGGGTCACAT ACACCATCAA TCCATCTTCT
    CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTTG GACGCATCGT GGCCAAAGCT
    GTGTATGACA ACCGCCTCCT GGAGTGCTAC TTTACTAGGT CCTTCTACAA ACACATTTTG
    GGCAAGTCTG TCAGATATAC AGATATGGAG AGTGAAGACT ACCACTTTTA CCAGGGCCTG
    GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ATCTCACTTT CAGCACTGAG
    GTCCAAGAGT TTGGAGTCTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG
    GTAACAGAGG AAAATAAGAA GGAATATGTA CACCTGGTGT GCCAGATGAG AATGACAGGA
    GCCATCCGCA AACAGCTGGC GGCTTTCTTA GAAGGTTTCT ATGAGATCAT TCCAAAGCGC
    CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTCA TATCAGGACT GCCCACAATC
    GACATTGACG ATTTGAAATC TAACACTGAA TATCACAAGT ACCAGTCCAA CTCTATTCAG
    ATCCAGTGGT TCTGGAGAGC ACTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC
    CAGTTTGTCA CAGGTACTTC CAAGGTGCCC CTGCAAGGTT TTGCTGCCCT TGAAGGCATG
    AATGGCATTC AGAAGTTTCA AATCCATCGA GATGACAGGT CCACAGACCG CCTGCCTTCA
    GCTCACACAT GTTTTAATCA GCTGGACCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC
    CACATGCTAC TATTGGCCAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35633
    Clone ID Related Accession (Same CDS sequence) XM_024987894.1 , XM_010821997.2 , XM_005228212.2 , XM_005228212.3 , XM_024987895.1
    Accession Version XM_010821997.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
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    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATAA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTCCACTT AGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGATTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT
    GTACTTGTGC GAAACTGTAT CCAGGCCATG ATTGACCCTT CCATGGATCC ATACCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CAAGCTACGA TGCTGGTGGC
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG CCGAAAAGAA TGCCCTTTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTGCACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TACAGATGTG
    ACTATGGAAA GTAGTCCCAG CTCATCCATC TCTATGGAGC ACCGGCTGGA TGTTGAATTA
    AGGGCATCAA GTTCCAGCAG CAGTAGCATC TCCTCTGGCC CTGGCCCTCG TCCTGGAGTC
    CAGTGTATTC CACAACGAGC CGCACTTCTC AAATCCATGT TGAATTTCCT CAAGAAGGCC
    ATTCAAGACC CTGCTTTCTC AGATGGCATA CGACATGTGA TGGATGGTTC TCTGCCTACC
    TCCCTGAAAC ACATCATCAG CAATGCAGAA TACTATGGCC CATCACTCTT TCTCCTAGCT
    ACTGAAGTGG TGACTGTGTT TGTATTTCAA GAACCATCAT TGCTTTCCTC ACTCCAGGAC
    AATGGATTGA CAGATGTCAT GCTACATGCA CTGCTTATCA AAGACGTTCC TGCTACCCGT
    GAAGTTCTTG GCTCCCTCCC AAATGTTTTC AGTGCACTCT GCTTGAATGC CCGAGGTCTG
    CAGTCATTTG TACAGTGTCA GCCTTTTGAA CGTCTCTTCA AAGTTCTCCT GTCTCCAGAT
    TACCTCCCAG CCATGCGGAG AAGGAGGAGT TCTGATCCTC TAGGGGATAC TGCCTCCAAC
    CTAGGAAGTG CTGTAGATGA GCTCATGAGA CATCAGCCTA CCCTCAAAAC AGATGCAACG
    ACTGCGATCA TCAAGTTACT TGAAGAAATC TGTAATCTTG GAAGAGACCC TAAATACATC
    TGTCAGAAGC CATCAATCCA GAAGGCAGAT GGCACTGCCG CTGCTCCTCC CCCAAGATCT
    AACCATGCTG CAGAAGAGGC CTCTAGTGAG GATGAGGAGG AAGAGGAAGT ACAGGCCATG
    CAGAGCTTTA ATTCTACCCA GCAGAATGAA ACTGAGCCTA ATCAGCAGGT TGTTGGTACA
    GAGGAGCGTA TTCCTATTCC CCTCATGGAT TACATCCTTA ATGTGATGAA ATTTGTGGAA
    TCTATTCTGA GCAACAATAC AACTGATGAC CACTGCCAGG AATTTGTGAA TCAGAAAGGA
    CTCTTGCCTT TGGTCACCAT TTTGGGTCTT CCCAATCTGC CCATTGACTT TCCCACGTCT
    GCAGCCTGTC AGGCTGTTGC AGGTGTCTGC AAATCCATAT TGACACTGTC ACATGAACCC
    AAAGTCCTCC AGGAAGGTCT CCTTCAGTTG GACTCCATTC TTTCCTCCTT GGAGCCCTTA
    CACCGCCCAA TAGAATCCCC CGGGGGCTCA GTGTTGTTGC GAGAACTGGC TTGTGCTGGC
    AATGTTGCTG ATGCTACCCT CTCAGCTCAA GCCACACCTC TACTACATGC ACTCACTGCT
    GCCCATGCTT ACATCATGAT GTTTGTTCAT ACTTGCAGGG TTGGACAGAG TGAAATTCGT
    TCCATCTCCA TAAACCAGTG GGGCTCTCAG TTGGGTCTGA GTGTTCTGAG CAAGCTGAGC
    CAGTTATACT GTTCCCTGGT ATGGGAAAGC ACTGTCCTCC TCTCCCTCTG TACCCCAAAC
    AGCTTGCCAT CTGGGTGTGA ATTTGGCCAG GCAGACATGC AGAAACTGGT TCCAAAGGAT
    GAGAAGACAG GTACGACCCA GGGCGGAAAA AGATCAGATG GGGATCAGGA TGGAACCACT
    GGAAGTATGG ATGCTTCTAC CCAGGGCTTG TTGGAAGGCA TTGGGCTTGA TGGTGACACA
    CTGGCTCCTA TGGAGACAGA CGAACCTACT GCTTCAGACT CTAAGGGCAA ATCTAAAATC
    ACACCTGCCA TGGCTGCCAG AATCAAGCAA ATCAAGCCAT TGTTGTCAGC TTCCTCTAGA
    TTAGGCAGAG CACTGGCTGA GCTGTTTGGA CTCCTTGTTA AACTTTGTGT GGGGTCTCCT
    GTCCGCCAGA GAAGAAGCCA TCATGCTGCC AGCACCACCA CAGCACCAAC ACCTGCTGCT
    CGATCAACGG CCTCAGCCCT CACTAAGCTC CTCACAAAAG GCTTATCATG GCAGCCTCCA
    CCATATACGC CTACTCCACG CTTCAGGCTG ACCTTCTTCA TCTGTTCAGT TGGTTTTACA
    TCCCCAATGC TGTTTGATGA GAGGAAGTAT CCCTACCACC TCATGCTGCA GAAATTTCTC
    TGCTCTGGAG GCCACAATGC CCTTTTTGAA ACATTCAACT GGGCTCTGTC TATGGGAGGA
    AAAGTTCCTG TTGCTGAGGG ATTAGAACAT TCAGACTTAC CTGATGGCAC AGGAGAATTC
    CTAGATGCCT GGCTAATGCT GGTGGAGAAG ATGGTAAATC CCACCACTGT GCTTGAATCT
    CCACATTCAC TGCCTGCCAA ATTGCCTGGA GGTGTTCAGA ACTTTCCACA GTTCAGTGCC
    CTCCGCTTCC TTGTGGTAAC TCAGAAGGCA GCATTTACTT GCATCAAGAA CCTGTGGAAC
    CGGAAACCCT TGAAAGTGTA TGGTGGGCGA ATGGCTGAGT CCATGCTAGC CATCCTGTGC
    CACATCCTCC GAGGAGAACC TGTGATTCGA GAGAGACTGA GCAAAGAAAA GGAGGGATCT
    CGAGGAGAAG AGGATACAGG ACAAGAAGAA GGTGGCTCTC GCCGGGAACC TCAAGTCAAC
    CAGCAACAAC TACAACAGCT CATGGACATG GGCTTCACAA GGGAACATGC CATGGAGGCC
    CTGTTGAACA CCAGTACCAT GGAGCAGGCC ACAGAGTACC TTCTAACCCA CCCTCCCCCG
    ATCATGGGAG GAGTTGTTCG GGATCTGAGT ATGTCTGAAG AGGACCAGAT GATGAGAGCG
    ATTGCCATGT CTCTGGGACA GGATATCCCA ATGGATCAAA GAGCAGAGTC ACCTGAGGAA
    GTTGCTTGCC GAAAGGAAGA GGAGGAACGG AAAGCTCGAG AAAAGCAGGA AGAGGAAGAG
    GCTAAATGTC TAGAGAAGTT CCAGGATGCT GATCCATTGG AGCAAGATGA GCTCCACACT
    TTCACAGATA CAATGTTGCC AGGCTGCTTT CACCTTCTTG ATGAGTTGCC AGACACTGTA
    TACCGAGTGT GTGATCTGAT CATGACAGCA ATCAAACGAA ATGGAGCAGA CTATCGAGAC
    ATGATTCTGA AGCAAGTAGT TAATCAGGTG TGGGAAGCTG CTGATGTATT GATCAAAGCT
    GCTCTTCCCC TGACAACAAG TGACACAAAA ACTGTATCAG AGTGGATCAG TCAGATGGCC
    ACTCTACCCC AGGCCTCTAA TTTGGCCACT AGAATCTTGC TTTTAACACT GCTTTTTGAG
    GAATTGAAGC TACCTTGTGC TTGGGTGGTT GAATCTAGTG GCATCCTTAA TGTCCTAATC
    AAACTCTTGG AAGTGGTTCA GCCCTGCCTA CAGGCCGCCA AGGAACAAAA GGAGGTCCAG
    ACCCCAAAGT GGATCACTCC AGTGTTGCTC CTGATTGATT TCTATGAAAA AACAGCCATT
    TCCTCAAAGA GGAGAGCCCA AATGACTAAG TATCTACAGT CTAACAACAA CAACTGGCGC
    TGGTTTGATG ACCGCTCTGG ACGTTGGTGT AGTTACAGTG CAAGCAACAA CAGCACTATT
    GATTCTGCCT GGAAATCTGG AGAGACAAGT GTGCGTTTCA CTGCAGGCCG AAGAAGATAC
    ACTGTCCAGT TCACTACAAT GGTACAGGTT AATGAGGAAA CAGGGAACCG GCGTCCTGTG
    ATGCTGACAC TCCTGAGGGT ACCCCGATTG AATAAAAATT CTAAAAACAG CAATGGACAG
    GAACTAGAGA AGACACTGGA AGAAAGCAAA GAAATGGATA TCAAACGTAA AGAAAATAAA
    ACCAGTGATA CCCCCTTGGC CCTAGACAGT ACAAATACTG AAAAGGAGAC AAGCCTGGAA
    GAAACAAAAA TTGGGGAGAT CTTGATCCAG GGCCTGACAG AGGATATGGT GACTGTTTTA
    ATCCGGGCCT GTGTGAGCAT GCTAGGCGTC CCTGTGGATC CAGACACTTT GCATGCCACC
    CTTCGCCTAT GCCTGAGGCT CACCCGAGAC CACAAATATG CCATGATGTT TGCAGAGCTG
    AAGAGTACTC GCATGATCTT AAATTTGACC CAGAGTTCAG GCTTCAATGG GTTTACTCCC
    CTGGTTACCC TTCTCTTAAG ACACATCATT GAGGACCCCT GCACCCTGCG CCATACCATG
    GAAAAGGTTG TTCGCTCAGC AGCTACAAGT GGAGCTGGTA GCACTACTTC TGGTGTTGTA
    TCTGGCAGCC TTGGCTCTCG GGAGATCAAC TACATCCTTC GTGTCCTTGG ACCAGCCGCA
    TGCCGCAACC CAGACATTTT CACAGAAGTG GCCAACTGCT GTATCCGCAT TGCCCTTCCA
    GCCCCTCGCG GCTCAGGAAC TGCTTCAGAT GATGAGTTTG AGAATCTCAG AATTAAAGGC
    CCGAATGCTG TACAGCTGGT GAAAACAACC CCTTTGAAGC CCTCACCTCT GCCTGTCATT
    CCTGATACTA TCAAGGAAGT GATCTATGAC ATGCTAAATG CCCTGGCTGC ATACCATGCT
    CCAGAGGAAG CAGACAAATC TGATCCTAAA CCAGGGGGTA TGACTCAAGA GGTTGGCCAG
    CTCCTGCAAG ACATGGGTGA TGACGTATAC CAGCAGTACC GGTCACTTAC CCGTCAGAGC
    AGTGACTTTG ATGCACAGAC AGGATTTTCC ATTAATAGTC AGGTCTTTGC TGCAGATGGT
    TCCTCCACTG AGACTTCCGC ATCTGGGGTA TCCCAAGGAG AAGTGTCAAC TCCGGAGGAG
    TCTCGGGATG GGAAGAAAGA TAAAGAAGGG GACCGGGCCT CTGAGGAAGG CAAGCAGAAA
    GGCAAGGGCA GCAAACCTTT AATGCCCACC TCCACTATTC TTCGGCTTCT GGCAGAGTTG
    GTGAGATCCT ATGTTGGTAT TGCTACCCTG ATTGCCAACT ACAGCTACAC TGTGGGCCAG
    TCTGAGCTGA TCAAAGAGGA CTGCAGTGTG CTAGCTTTTG TCTTGGACCA CCTCCTCCCG
    CATACCCAGA ATGCAGAAGA CAAAGACACA CCGGCGCTGG CCCGCCTGTT CCTCGCAAGC
    CTGGCTGCCT CAGGGAGTGG CACAGATGCC CAGGTGGCCC TTGTGAATGA AGTAAAAGCA
    GCCCTGGGAA GGGCACTGGC TATGGCTGAG AGTACAGAGA AACATGCCAG ACTTCAGGCA
    GTGATGTGTA TCATCAGTAC TATCATGGAG TCCTGCCCCT CCACCTCCAG TTTCTACAGC
    AGTGCAACAG CCAAGACCCA ACACAACGGC ATGAACAACA TCATTCGACT CTTCCTGAAG
    AAGGGACTGG TTAATGACCT GGCCAGAGTG CCTCACAGCC TGGACCTGTC CAGTCCCAAC
    ATGGCCAACA CTGTCAATGC TGCTCTGAAG CCTTTGGAAA CACTTTCCCG GATTGTGAAC
    CAGCCCAGTA GCCTTTTTGG CAGTAAGAGT GCTTCTAGCA AGAGCAAGTC TGAGCAGGAT
    GCCCAAGGAG CTGCTCAGGA CTCTAATAGC AACCAACAGG ACCCAGGCGA GCCTGGGGAA
    ACAGAAGTGC AGGAGGAGGA TCATGATGTC ACTCAGACAG AGGTGGCAGA TGGGGATATC
    ATGGATGGGG AGGCTGAAAC CGACTCAGTG GTGATTGCTG GGCAGCCTGA GGTGCTCAGT
    TCACAAGAGA TGCAGGTTGA GAATGAGCTG GAGGACCTGA TAGATGAGTT GCTTGAGAGG
    GATGGCGGAT CTGGGAACAG TACAATTATA GTGAGCAGAA GTGGAGAGGA TGAATCACAA
    GAGGACGTGC TGATGGATGA AGCTCCTTCC AACCTCAGCC AAGCTTCCAC CTTGCAGGCC
    AACCGAGAAG ATTCCATGAA TATCCTGGAC CCTGAGGATG AGGAGGAGCA CACTCAGGAA
    GAGGACAGCA GTGGCAGTAA CGAGGATGAG GATGATAGTC AGGATGAAGA GGAGGAGGAG
    GAGGAAGATG AGGAAGATGA TCAGGAGGAT GATGAAGGTG AAGAGGGAGA TGAAGACGAT
    GACGACGATG GCTCTGAAAT GGAATTGGAT GAGGATTACC CTGATATGAA CGCTTCTCCC
    TTGGTCCGAT TTGAGCGCTT TGACCGGGAG GATGATCTCA TCATTGAGTT TGACAACATG
    TTCTCCAGTG CTACAGACAT CCCCCCATCC CCAGGAAATA TCCCTACCAC CCACCCACTG
    ATGGTACGCC ATGCAGATCA CAGTTCCCTG ACACTGGGCA GTGGCTCCTC TACAACACGT
    CTCACCCAGG GCATTGGGCG CAGTCAAAGG ACCCTGAGAC AGCTGACAGC CAACACAGGC
    CATACCATTC ACGTTCACTA CCCTGGGAAT CGCCAGCCCA ATCCTCCTCT TATACTCCAG
    AGGTTGCTTG GTCCCTCTGC TGCTGCTGAC ATCCTTCAGC TGAGCAGCAG CCTTCCCCTA
    CAAAGCCGAG GCCGAGCCCG CCTCCTAGTG GGCAACGATG ACGTCCACAT CATCGCCCGG
    TCTGATGATG AACTACTGGA TGACTTTTTC CATGATCAGA GCACAGCTAC CAGCCAAGCA
    GGGACCCTAT CCAGTATCCC TACAGCCCTG ACTCGCTGGA CAGAGGAATG CAAAGTTCTT
    GATGCTGAGA GCATGCATGA CTGTGTTTCA GTTGTTAAAG TGCCCATTGT CAATCATCTG
    GAATTCCTGA GGGATGAGGA GCTGGAAGAA AGGAGGGAGA AAAGGAGGAA ACAACTAGCT
    GAGGAAGAAA CAAAGATAAC CGACAAAGGC AAAGAAGATA AGGAGAACAG GGATCAGAGT
    GCACAGTGTG CTGCATCTAA GACAAATGAC TCCACTGAAC AGAACCTCTC AGATGGGACT
    CCTATGCCTG ACAGCTACCC AGCAACCCCA TCTTCAACTG ATGTAGCTAC ATCTGAGCCC
    AGGGAGACCC TTGTCACTCT GCAGCCCTCC CAACAGCAAC CAACACTCCC AACCCCACCA
    GCCTTGGGAG AGATTCCTCA AGAGCTGCAG TCCCCAATTG GAGAAGGTGG GAGCTCTACA
    CAGCTATTGA TGCCTGTAGA GCCAGAGGAA TTGGGTCCCA CAAGGCCAAG TGGAGAAGCA
    GAAACAACTC AGATGGAGCT ATCTCCAGCT CCCACTATAA CCTCTCTTTC CCCAGAGAGA
    GCTGAAGATT CTGATGCTCT GACAGCTGTC AGCAGTCAAC TGGAAGGCTC TCCCATGGAT
    ACTAGCAGCC TGGCTTCCTG TACCCTGGAG GAGGCTGTGG GTGACGCCCC AGCAACTGGC
    AGTTCTGAGC AGCCCACAGC AGGCAGCTCC ACTCCTGGGG ATGCCCCACC AGTTGTGACA
    GAAGTACAAG GCAGGGGTGA TGGGTCAGGG GAACCTACCC AGCCCCCTGA GGACAGCTCT
    CCCCCTGCAT CCTCTGAGAG TTCTTCCACC AGAGATTCCG CGGTGGCCAT TTCTGGAGCA
    GATTCCCGGG GAATCCTGGA AGAGCCATTG CCTTCAACAA GCAGTGAAGA AGAAGATCCT
    CTTGCAGGTA TTAGTCTCCC CGAAGGTGTG GACCCCTCTT TTCTGGCCGC TCTGCCTGAT
    GACATTCGCC GAGAAGTCCT ACAGAACCAG CTGGGCATTC GTCCACCAAC ACGAACTGCC
    CCTTCAACAA ATAACTCAGC TCCTGCAGTG GTGGGGAATC CTGGTGTCAC CGAAGTGAGC
    CCTGAGTTCC TGGCTGCTCT GCCTCCAGCC ATCCAGGAGG AAGTACTGGC ACAGCAGAGA
    GCTGAGCAAC AGCGACGGGA ACTAGCACAA AATGCCAGCT CAGACACCCC CATGGACCCT
    GTGACCTTCA TCCAGACTCT GCCCTCAGAC CTGCGCCGTA GTGTCCTAGA GGACATGGAG
    GACAGTGTGT TGGCCGTGAT GCCACCTGAC ATCGCAGCTG AAGCTCAAGC CCTGAGGCGA
    GAGCAAGAAG CCCGGCAGAG GCAGCTCATG CATGAGCGAC TTTTTGGGCA CAGCAGCACC
    TCTGCACTCT CTGCCATTCT CCGAAGCCCA GCCTTCACCA GTCGCCTGAG TGGCAACCGT
    GGGGTCCAAT ACACTCGCCT TGCTGTGCAA AGAGGTGGCA CCTTCCAGAT GGGGGGTAGC
    AGCAGCCATA ATAGGCCTTC TGGCAGTAAT GTGGACACCC TCCTACGTCT TCGAGGACGG
    CTCCTCCTGG ATCACGAAGC CCTGTCTTGT CTCCTGGTCC TACTTTTTGT GGATGAGCCA
    AAACTCAATA CTAGCCGTCT TCACCGAGTA CTGAGAAATC TCTGCTACCA TGCCCAGACC
    CGCCACTGGG TTATCCGTAG CCTGCTTTCC ATCTTGCAGC GAAGCAGTGA GAGTGAGCTT
    TGCATTGAAA CACCCAAGCT CTCCTCAAGT GAGGAAAAGG GCAAAAAGTC AAGCAAGAGC
    TGTGGGTCAA GCAGCCATGA GAACCGGCCC CTGGACCTGC TACACAAGAT GGAGTCTAAG
    AGTTCCAACC AGCTTTCCTG GCTCTCAGTA TCCATGGATG CAGCCCTAGG CTGCAGGACT
    AATATATTCC AGATCCAGCG TTCAGGGGGT CGCAAACATA CTGAGAAACA TGCGAGTAGT
    GGCTCCACTG TCCATATCCA CCCCCAGGCT GCTCCTGTTG TCTGCAGACA TGTTCTGGAT
    ACACTCATTC AATTGGCCAA GGTGTTTCCC AGTCACTTCA CACAGCAGCG GACCAAAGAA
    ACAAACTGTG AGAGTGATCG GGAGAGGGGC AGTAAGGCCT GCAGCCCATG CTCTTCACAG
    TCCACAAGCA GTGGCATTTG CACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG
    AATGTCAGCC GGAAAGGCAA GAACTCCGTG AAGTCAGTGC CAGTGAGCGC TGGTGGTGAG
    GGGGAAACCT CCCCATACAG CCTCGAAGCT TCTCCACTGG GGCAACTCAT GAACATGTTG
    TCACACCCAG TCATCCGCCG GAGCTCTCTC TTAACTGAGA AGCTCCTCAG ACTTCTTTCT
    CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTGTCGGAAG CACAGGCTAA TTCTGGCAGC
    AGTGCTTCCT CCACCACTGT TGCCACTTCA ACCACATCTA CCACCACTAT CAGTGCCACC
    TCCAGCACAT CTACTCTGCC TGCTGCAACC ACCCCTGTCA CTTCTGTTCC AGCCCTGGTT
    GCTGCCACGG CTATATCCAC CATTGCCGTA GCCGCTTCAA CCACAGTGAC TACCCCCACA
    ACTGCTACCA CTACTGTTTC AACTGCTACT ACTACTAAGG CCAGCAAATC TCCAGCTAAA
    GTTGGTGATA TCGGCAGCAG CAGCAGCACA GACTTTAAGA TGGTATCCTC TGGCCTCACT
    GAAAATCAGC TCCAGCTCTC TGTGGAAGTG TTGACATCCC ATTCTTGTTC TGAAGAAGGC
    CTAGAAGATG CAGCAAATGT GTTACTGCAG CTCTCTCGGG GGGACCCTGG GACCCGGGAC
    ACTGTTTTGA AGTTGCTACT GAATGGAGCC CGCCATCTGG GTTATACGCT TTGTAAACAG
    ATAGGAACTC TTCTGGCTGA GCTACGGGAA TACAATCTGG AACAGCAGCG GCGAGCCCAG
    TGTGAAACCC TCTCCCCTGA TGGCATGCCT GAGGAACAGC CACAGACCAC CAAGCTGAAA
    GGCAAAATGC AGAGCAGGTT TGACGTGGCT GAGAATGTGG TAATCGTGGC ATCTCAGAAG
    CGGCCTCTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA
    TCTACCCAGA AGTTCTTCTT GAGGGTACTG CAGGTCATCA TCCAGCTCCG GGATGACACG
    CGCCGTGCTA ACAAGAAGGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA
    GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA
    ATGGTACGTG AGGGTCAGAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCAAGC
    CAGTCAGAGG CATCTGTCCG AAGGGAGGAG TCACCCATGG ATGTGGACCA GCCATCTCCC
    AGTGCTCAAG ATACTCAGTC CATTGGCTCA GATGGAACTC CACAGGGAGA GAAGGAGAAG
    GAGGAGAGGC CACCTGAGTT ACCCCTGCTC AGTGAGCAGC TGAACTTGGA TGAGCTGTGG
    GACATGCTGG GAGAGTGTCT AAAGGAACTG GAGGAATCCC ATGACCAGCA TGCGGTGCTA
    GTGCTACAAC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CAACAGAGCG GGAGAGCAAG
    CCTCCTGTCC GGGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCACCTCCA
    CTCTCCCCTG CCCCTTTAAC CCCAGCCACT CCTTCCTCCC TTGACCCGTT CTTCTCCCGG
    GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTTCTTCGT
    TTTGCAGAGA CTCATCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC CACCCACCTC
    GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT CGATGTCAAG
    CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTGGACGAGG GACTCCGGAA AGAAGACATG
    GCTGTGCACG TTCGTCGTGA TCATGTCTTT GAAGACTCCT ATCGTGAGCT GCACCGGAAA
    TCCCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT
    GCTGGAGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG
    TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGGGTCACAT ACACCATCAA TCCATCTTCT
    CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTTG GACGCATCGT GGCCAAAGCT
    GTGTATGACA ACCGCCTCCT GGAGTGCTAC TTTACTAGGT CCTTCTACAA ACACATTTTG
    GGCAAGTCTG TCAGATATAC AGATATGGAG AGTGAAGACT ACCACTTTTA CCAGGGCCTG
    GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ATCTCACTTT CAGCACTGAG
    GTCCAAGAGT TTGGAGTCTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG
    GTAACAGAGG AAAATAAGAA GGAATATGTA CACCTGGTGT GCCAGATGAG AATGACAGGA
    GCCATCCGCA AACAGCTGGC GGCTTTCTTA GAAGGTTTCT ATGAGATCAT TCCAAAGCGC
    CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTCA TATCAGGACT GCCCACAATC
    GACATTGACG ATTTGAAATC TAACACTGAA TATCACAAGT ACCAGTCCAA CTCTATTCAG
    ATCCAGTGGT TCTGGAGAGC ACTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC
    CAGTTTGTCA CAGGTACTTC CAAGGTGCCC CTGCAAGGTT TTGCTGCCCT TGAAGGCATG
    AATGGCATTC AGAAGTTTCA AATCCATCGA GATGACAGGT CCACAGACCG CCTGCCTTCA
    GCTCACACAT GTTTTAATCA GCTGGACCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC
    CACATGCTAC TATTGGCCAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820299.1
    CDS734..13987
    Translation

    Target ORF information:

    RefSeq Version XM_010821997.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821997.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATAA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTCCACTT AGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGATTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT
    GTACTTGTGC GAAACTGTAT CCAGGCCATG ATTGACCCTT CCATGGATCC ATACCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CAAGCTACGA TGCTGGTGGC
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG CCGAAAAGAA TGCCCTTTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTGCACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TACAGATGTG
    ACTATGGAAA GTAGTCCCAG CTCATCCATC TCTATGGAGC ACCGGCTGGA TGTTGAATTA
    AGGGCATCAA GTTCCAGCAG CAGTAGCATC TCCTCTGGCC CTGGCCCTCG TCCTGGAGTC
    CAGTGTATTC CACAACGAGC CGCACTTCTC AAATCCATGT TGAATTTCCT CAAGAAGGCC
    ATTCAAGACC CTGCTTTCTC AGATGGCATA CGACATGTGA TGGATGGTTC TCTGCCTACC
    TCCCTGAAAC ACATCATCAG CAATGCAGAA TACTATGGCC CATCACTCTT TCTCCTAGCT
    ACTGAAGTGG TGACTGTGTT TGTATTTCAA GAACCATCAT TGCTTTCCTC ACTCCAGGAC
    AATGGATTGA CAGATGTCAT GCTACATGCA CTGCTTATCA AAGACGTTCC TGCTACCCGT
    GAAGTTCTTG GCTCCCTCCC AAATGTTTTC AGTGCACTCT GCTTGAATGC CCGAGGTCTG
    CAGTCATTTG TACAGTGTCA GCCTTTTGAA CGTCTCTTCA AAGTTCTCCT GTCTCCAGAT
    TACCTCCCAG CCATGCGGAG AAGGAGGAGT TCTGATCCTC TAGGGGATAC TGCCTCCAAC
    CTAGGAAGTG CTGTAGATGA GCTCATGAGA CATCAGCCTA CCCTCAAAAC AGATGCAACG
    ACTGCGATCA TCAAGTTACT TGAAGAAATC TGTAATCTTG GAAGAGACCC TAAATACATC
    TGTCAGAAGC CATCAATCCA GAAGGCAGAT GGCACTGCCG CTGCTCCTCC CCCAAGATCT
    AACCATGCTG CAGAAGAGGC CTCTAGTGAG GATGAGGAGG AAGAGGAAGT ACAGGCCATG
    CAGAGCTTTA ATTCTACCCA GCAGAATGAA ACTGAGCCTA ATCAGCAGGT TGTTGGTACA
    GAGGAGCGTA TTCCTATTCC CCTCATGGAT TACATCCTTA ATGTGATGAA ATTTGTGGAA
    TCTATTCTGA GCAACAATAC AACTGATGAC CACTGCCAGG AATTTGTGAA TCAGAAAGGA
    CTCTTGCCTT TGGTCACCAT TTTGGGTCTT CCCAATCTGC CCATTGACTT TCCCACGTCT
    GCAGCCTGTC AGGCTGTTGC AGGTGTCTGC AAATCCATAT TGACACTGTC ACATGAACCC
    AAAGTCCTCC AGGAAGGTCT CCTTCAGTTG GACTCCATTC TTTCCTCCTT GGAGCCCTTA
    CACCGCCCAA TAGAATCCCC CGGGGGCTCA GTGTTGTTGC GAGAACTGGC TTGTGCTGGC
    AATGTTGCTG ATGCTACCCT CTCAGCTCAA GCCACACCTC TACTACATGC ACTCACTGCT
    GCCCATGCTT ACATCATGAT GTTTGTTCAT ACTTGCAGGG TTGGACAGAG TGAAATTCGT
    TCCATCTCCA TAAACCAGTG GGGCTCTCAG TTGGGTCTGA GTGTTCTGAG CAAGCTGAGC
    CAGTTATACT GTTCCCTGGT ATGGGAAAGC ACTGTCCTCC TCTCCCTCTG TACCCCAAAC
    AGCTTGCCAT CTGGGTGTGA ATTTGGCCAG GCAGACATGC AGAAACTGGT TCCAAAGGAT
    GAGAAGACAG GTACGACCCA GGGCGGAAAA AGATCAGATG GGGATCAGGA TGGAACCACT
    GGAAGTATGG ATGCTTCTAC CCAGGGCTTG TTGGAAGGCA TTGGGCTTGA TGGTGACACA
    CTGGCTCCTA TGGAGACAGA CGAACCTACT GCTTCAGACT CTAAGGGCAA ATCTAAAATC
    ACACCTGCCA TGGCTGCCAG AATCAAGCAA ATCAAGCCAT TGTTGTCAGC TTCCTCTAGA
    TTAGGCAGAG CACTGGCTGA GCTGTTTGGA CTCCTTGTTA AACTTTGTGT GGGGTCTCCT
    GTCCGCCAGA GAAGAAGCCA TCATGCTGCC AGCACCACCA CAGCACCAAC ACCTGCTGCT
    CGATCAACGG CCTCAGCCCT CACTAAGCTC CTCACAAAAG GCTTATCATG GCAGCCTCCA
    CCATATACGC CTACTCCACG CTTCAGGCTG ACCTTCTTCA TCTGTTCAGT TGGTTTTACA
    TCCCCAATGC TGTTTGATGA GAGGAAGTAT CCCTACCACC TCATGCTGCA GAAATTTCTC
    TGCTCTGGAG GCCACAATGC CCTTTTTGAA ACATTCAACT GGGCTCTGTC TATGGGAGGA
    AAAGTTCCTG TTGCTGAGGG ATTAGAACAT TCAGACTTAC CTGATGGCAC AGGAGAATTC
    CTAGATGCCT GGCTAATGCT GGTGGAGAAG ATGGTAAATC CCACCACTGT GCTTGAATCT
    CCACATTCAC TGCCTGCCAA ATTGCCTGGA GGTGTTCAGA ACTTTCCACA GTTCAGTGCC
    CTCCGCTTCC TTGTGGTAAC TCAGAAGGCA GCATTTACTT GCATCAAGAA CCTGTGGAAC
    CGGAAACCCT TGAAAGTGTA TGGTGGGCGA ATGGCTGAGT CCATGCTAGC CATCCTGTGC
    CACATCCTCC GAGGAGAACC TGTGATTCGA GAGAGACTGA GCAAAGAAAA GGAGGGATCT
    CGAGGAGAAG AGGATACAGG ACAAGAAGAA GGTGGCTCTC GCCGGGAACC TCAAGTCAAC
    CAGCAACAAC TACAACAGCT CATGGACATG GGCTTCACAA GGGAACATGC CATGGAGGCC
    CTGTTGAACA CCAGTACCAT GGAGCAGGCC ACAGAGTACC TTCTAACCCA CCCTCCCCCG
    ATCATGGGAG GAGTTGTTCG GGATCTGAGT ATGTCTGAAG AGGACCAGAT GATGAGAGCG
    ATTGCCATGT CTCTGGGACA GGATATCCCA ATGGATCAAA GAGCAGAGTC ACCTGAGGAA
    GTTGCTTGCC GAAAGGAAGA GGAGGAACGG AAAGCTCGAG AAAAGCAGGA AGAGGAAGAG
    GCTAAATGTC TAGAGAAGTT CCAGGATGCT GATCCATTGG AGCAAGATGA GCTCCACACT
    TTCACAGATA CAATGTTGCC AGGCTGCTTT CACCTTCTTG ATGAGTTGCC AGACACTGTA
    TACCGAGTGT GTGATCTGAT CATGACAGCA ATCAAACGAA ATGGAGCAGA CTATCGAGAC
    ATGATTCTGA AGCAAGTAGT TAATCAGGTG TGGGAAGCTG CTGATGTATT GATCAAAGCT
    GCTCTTCCCC TGACAACAAG TGACACAAAA ACTGTATCAG AGTGGATCAG TCAGATGGCC
    ACTCTACCCC AGGCCTCTAA TTTGGCCACT AGAATCTTGC TTTTAACACT GCTTTTTGAG
    GAATTGAAGC TACCTTGTGC TTGGGTGGTT GAATCTAGTG GCATCCTTAA TGTCCTAATC
    AAACTCTTGG AAGTGGTTCA GCCCTGCCTA CAGGCCGCCA AGGAACAAAA GGAGGTCCAG
    ACCCCAAAGT GGATCACTCC AGTGTTGCTC CTGATTGATT TCTATGAAAA AACAGCCATT
    TCCTCAAAGA GGAGAGCCCA AATGACTAAG TATCTACAGT CTAACAACAA CAACTGGCGC
    TGGTTTGATG ACCGCTCTGG ACGTTGGTGT AGTTACAGTG CAAGCAACAA CAGCACTATT
    GATTCTGCCT GGAAATCTGG AGAGACAAGT GTGCGTTTCA CTGCAGGCCG AAGAAGATAC
    ACTGTCCAGT TCACTACAAT GGTACAGGTT AATGAGGAAA CAGGGAACCG GCGTCCTGTG
    ATGCTGACAC TCCTGAGGGT ACCCCGATTG AATAAAAATT CTAAAAACAG CAATGGACAG
    GAACTAGAGA AGACACTGGA AGAAAGCAAA GAAATGGATA TCAAACGTAA AGAAAATAAA
    ACCAGTGATA CCCCCTTGGC CCTAGACAGT ACAAATACTG AAAAGGAGAC AAGCCTGGAA
    GAAACAAAAA TTGGGGAGAT CTTGATCCAG GGCCTGACAG AGGATATGGT GACTGTTTTA
    ATCCGGGCCT GTGTGAGCAT GCTAGGCGTC CCTGTGGATC CAGACACTTT GCATGCCACC
    CTTCGCCTAT GCCTGAGGCT CACCCGAGAC CACAAATATG CCATGATGTT TGCAGAGCTG
    AAGAGTACTC GCATGATCTT AAATTTGACC CAGAGTTCAG GCTTCAATGG GTTTACTCCC
    CTGGTTACCC TTCTCTTAAG ACACATCATT GAGGACCCCT GCACCCTGCG CCATACCATG
    GAAAAGGTTG TTCGCTCAGC AGCTACAAGT GGAGCTGGTA GCACTACTTC TGGTGTTGTA
    TCTGGCAGCC TTGGCTCTCG GGAGATCAAC TACATCCTTC GTGTCCTTGG ACCAGCCGCA
    TGCCGCAACC CAGACATTTT CACAGAAGTG GCCAACTGCT GTATCCGCAT TGCCCTTCCA
    GCCCCTCGCG GCTCAGGAAC TGCTTCAGAT GATGAGTTTG AGAATCTCAG AATTAAAGGC
    CCGAATGCTG TACAGCTGGT GAAAACAACC CCTTTGAAGC CCTCACCTCT GCCTGTCATT
    CCTGATACTA TCAAGGAAGT GATCTATGAC ATGCTAAATG CCCTGGCTGC ATACCATGCT
    CCAGAGGAAG CAGACAAATC TGATCCTAAA CCAGGGGGTA TGACTCAAGA GGTTGGCCAG
    CTCCTGCAAG ACATGGGTGA TGACGTATAC CAGCAGTACC GGTCACTTAC CCGTCAGAGC
    AGTGACTTTG ATGCACAGAC AGGATTTTCC ATTAATAGTC AGGTCTTTGC TGCAGATGGT
    TCCTCCACTG AGACTTCCGC ATCTGGGGTA TCCCAAGGAG AAGTGTCAAC TCCGGAGGAG
    TCTCGGGATG GGAAGAAAGA TAAAGAAGGG GACCGGGCCT CTGAGGAAGG CAAGCAGAAA
    GGCAAGGGCA GCAAACCTTT AATGCCCACC TCCACTATTC TTCGGCTTCT GGCAGAGTTG
    GTGAGATCCT ATGTTGGTAT TGCTACCCTG ATTGCCAACT ACAGCTACAC TGTGGGCCAG
    TCTGAGCTGA TCAAAGAGGA CTGCAGTGTG CTAGCTTTTG TCTTGGACCA CCTCCTCCCG
    CATACCCAGA ATGCAGAAGA CAAAGACACA CCGGCGCTGG CCCGCCTGTT CCTCGCAAGC
    CTGGCTGCCT CAGGGAGTGG CACAGATGCC CAGGTGGCCC TTGTGAATGA AGTAAAAGCA
    GCCCTGGGAA GGGCACTGGC TATGGCTGAG AGTACAGAGA AACATGCCAG ACTTCAGGCA
    GTGATGTGTA TCATCAGTAC TATCATGGAG TCCTGCCCCT CCACCTCCAG TTTCTACAGC
    AGTGCAACAG CCAAGACCCA ACACAACGGC ATGAACAACA TCATTCGACT CTTCCTGAAG
    AAGGGACTGG TTAATGACCT GGCCAGAGTG CCTCACAGCC TGGACCTGTC CAGTCCCAAC
    ATGGCCAACA CTGTCAATGC TGCTCTGAAG CCTTTGGAAA CACTTTCCCG GATTGTGAAC
    CAGCCCAGTA GCCTTTTTGG CAGTAAGAGT GCTTCTAGCA AGAGCAAGTC TGAGCAGGAT
    GCCCAAGGAG CTGCTCAGGA CTCTAATAGC AACCAACAGG ACCCAGGCGA GCCTGGGGAA
    ACAGAAGTGC AGGAGGAGGA TCATGATGTC ACTCAGACAG AGGTGGCAGA TGGGGATATC
    ATGGATGGGG AGGCTGAAAC CGACTCAGTG GTGATTGCTG GGCAGCCTGA GGTGCTCAGT
    TCACAAGAGA TGCAGGTTGA GAATGAGCTG GAGGACCTGA TAGATGAGTT GCTTGAGAGG
    GATGGCGGAT CTGGGAACAG TACAATTATA GTGAGCAGAA GTGGAGAGGA TGAATCACAA
    GAGGACGTGC TGATGGATGA AGCTCCTTCC AACCTCAGCC AAGCTTCCAC CTTGCAGGCC
    AACCGAGAAG ATTCCATGAA TATCCTGGAC CCTGAGGATG AGGAGGAGCA CACTCAGGAA
    GAGGACAGCA GTGGCAGTAA CGAGGATGAG GATGATAGTC AGGATGAAGA GGAGGAGGAG
    GAGGAAGATG AGGAAGATGA TCAGGAGGAT GATGAAGGTG AAGAGGGAGA TGAAGACGAT
    GACGACGATG GCTCTGAAAT GGAATTGGAT GAGGATTACC CTGATATGAA CGCTTCTCCC
    TTGGTCCGAT TTGAGCGCTT TGACCGGGAG GATGATCTCA TCATTGAGTT TGACAACATG
    TTCTCCAGTG CTACAGACAT CCCCCCATCC CCAGGAAATA TCCCTACCAC CCACCCACTG
    ATGGTACGCC ATGCAGATCA CAGTTCCCTG ACACTGGGCA GTGGCTCCTC TACAACACGT
    CTCACCCAGG GCATTGGGCG CAGTCAAAGG ACCCTGAGAC AGCTGACAGC CAACACAGGC
    CATACCATTC ACGTTCACTA CCCTGGGAAT CGCCAGCCCA ATCCTCCTCT TATACTCCAG
    AGGTTGCTTG GTCCCTCTGC TGCTGCTGAC ATCCTTCAGC TGAGCAGCAG CCTTCCCCTA
    CAAAGCCGAG GCCGAGCCCG CCTCCTAGTG GGCAACGATG ACGTCCACAT CATCGCCCGG
    TCTGATGATG AACTACTGGA TGACTTTTTC CATGATCAGA GCACAGCTAC CAGCCAAGCA
    GGGACCCTAT CCAGTATCCC TACAGCCCTG ACTCGCTGGA CAGAGGAATG CAAAGTTCTT
    GATGCTGAGA GCATGCATGA CTGTGTTTCA GTTGTTAAAG TGCCCATTGT CAATCATCTG
    GAATTCCTGA GGGATGAGGA GCTGGAAGAA AGGAGGGAGA AAAGGAGGAA ACAACTAGCT
    GAGGAAGAAA CAAAGATAAC CGACAAAGGC AAAGAAGATA AGGAGAACAG GGATCAGAGT
    GCACAGTGTG CTGCATCTAA GACAAATGAC TCCACTGAAC AGAACCTCTC AGATGGGACT
    CCTATGCCTG ACAGCTACCC AGCAACCCCA TCTTCAACTG ATGTAGCTAC ATCTGAGCCC
    AGGGAGACCC TTGTCACTCT GCAGCCCTCC CAACAGCAAC CAACACTCCC AACCCCACCA
    GCCTTGGGAG AGATTCCTCA AGAGCTGCAG TCCCCAATTG GAGAAGGTGG GAGCTCTACA
    CAGCTATTGA TGCCTGTAGA GCCAGAGGAA TTGGGTCCCA CAAGGCCAAG TGGAGAAGCA
    GAAACAACTC AGATGGAGCT ATCTCCAGCT CCCACTATAA CCTCTCTTTC CCCAGAGAGA
    GCTGAAGATT CTGATGCTCT GACAGCTGTC AGCAGTCAAC TGGAAGGCTC TCCCATGGAT
    ACTAGCAGCC TGGCTTCCTG TACCCTGGAG GAGGCTGTGG GTGACGCCCC AGCAACTGGC
    AGTTCTGAGC AGCCCACAGC AGGCAGCTCC ACTCCTGGGG ATGCCCCACC AGTTGTGACA
    GAAGTACAAG GCAGGGGTGA TGGGTCAGGG GAACCTACCC AGCCCCCTGA GGACAGCTCT
    CCCCCTGCAT CCTCTGAGAG TTCTTCCACC AGAGATTCCG CGGTGGCCAT TTCTGGAGCA
    GATTCCCGGG GAATCCTGGA AGAGCCATTG CCTTCAACAA GCAGTGAAGA AGAAGATCCT
    CTTGCAGGTA TTAGTCTCCC CGAAGGTGTG GACCCCTCTT TTCTGGCCGC TCTGCCTGAT
    GACATTCGCC GAGAAGTCCT ACAGAACCAG CTGGGCATTC GTCCACCAAC ACGAACTGCC
    CCTTCAACAA ATAACTCAGC TCCTGCAGTG GTGGGGAATC CTGGTGTCAC CGAAGTGAGC
    CCTGAGTTCC TGGCTGCTCT GCCTCCAGCC ATCCAGGAGG AAGTACTGGC ACAGCAGAGA
    GCTGAGCAAC AGCGACGGGA ACTAGCACAA AATGCCAGCT CAGACACCCC CATGGACCCT
    GTGACCTTCA TCCAGACTCT GCCCTCAGAC CTGCGCCGTA GTGTCCTAGA GGACATGGAG
    GACAGTGTGT TGGCCGTGAT GCCACCTGAC ATCGCAGCTG AAGCTCAAGC CCTGAGGCGA
    GAGCAAGAAG CCCGGCAGAG GCAGCTCATG CATGAGCGAC TTTTTGGGCA CAGCAGCACC
    TCTGCACTCT CTGCCATTCT CCGAAGCCCA GCCTTCACCA GTCGCCTGAG TGGCAACCGT
    GGGGTCCAAT ACACTCGCCT TGCTGTGCAA AGAGGTGGCA CCTTCCAGAT GGGGGGTAGC
    AGCAGCCATA ATAGGCCTTC TGGCAGTAAT GTGGACACCC TCCTACGTCT TCGAGGACGG
    CTCCTCCTGG ATCACGAAGC CCTGTCTTGT CTCCTGGTCC TACTTTTTGT GGATGAGCCA
    AAACTCAATA CTAGCCGTCT TCACCGAGTA CTGAGAAATC TCTGCTACCA TGCCCAGACC
    CGCCACTGGG TTATCCGTAG CCTGCTTTCC ATCTTGCAGC GAAGCAGTGA GAGTGAGCTT
    TGCATTGAAA CACCCAAGCT CTCCTCAAGT GAGGAAAAGG GCAAAAAGTC AAGCAAGAGC
    TGTGGGTCAA GCAGCCATGA GAACCGGCCC CTGGACCTGC TACACAAGAT GGAGTCTAAG
    AGTTCCAACC AGCTTTCCTG GCTCTCAGTA TCCATGGATG CAGCCCTAGG CTGCAGGACT
    AATATATTCC AGATCCAGCG TTCAGGGGGT CGCAAACATA CTGAGAAACA TGCGAGTAGT
    GGCTCCACTG TCCATATCCA CCCCCAGGCT GCTCCTGTTG TCTGCAGACA TGTTCTGGAT
    ACACTCATTC AATTGGCCAA GGTGTTTCCC AGTCACTTCA CACAGCAGCG GACCAAAGAA
    ACAAACTGTG AGAGTGATCG GGAGAGGGGC AGTAAGGCCT GCAGCCCATG CTCTTCACAG
    TCCACAAGCA GTGGCATTTG CACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG
    AATGTCAGCC GGAAAGGCAA GAACTCCGTG AAGTCAGTGC CAGTGAGCGC TGGTGGTGAG
    GGGGAAACCT CCCCATACAG CCTCGAAGCT TCTCCACTGG GGCAACTCAT GAACATGTTG
    TCACACCCAG TCATCCGCCG GAGCTCTCTC TTAACTGAGA AGCTCCTCAG ACTTCTTTCT
    CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTGTCGGAAG CACAGGCTAA TTCTGGCAGC
    AGTGCTTCCT CCACCACTGT TGCCACTTCA ACCACATCTA CCACCACTAT CAGTGCCACC
    TCCAGCACAT CTACTCTGCC TGCTGCAACC ACCCCTGTCA CTTCTGTTCC AGCCCTGGTT
    GCTGCCACGG CTATATCCAC CATTGCCGTA GCCGCTTCAA CCACAGTGAC TACCCCCACA
    ACTGCTACCA CTACTGTTTC AACTGCTACT ACTACTAAGG CCAGCAAATC TCCAGCTAAA
    GTTGGTGATA TCGGCAGCAG CAGCAGCACA GACTTTAAGA TGGTATCCTC TGGCCTCACT
    GAAAATCAGC TCCAGCTCTC TGTGGAAGTG TTGACATCCC ATTCTTGTTC TGAAGAAGGC
    CTAGAAGATG CAGCAAATGT GTTACTGCAG CTCTCTCGGG GGGACCCTGG GACCCGGGAC
    ACTGTTTTGA AGTTGCTACT GAATGGAGCC CGCCATCTGG GTTATACGCT TTGTAAACAG
    ATAGGAACTC TTCTGGCTGA GCTACGGGAA TACAATCTGG AACAGCAGCG GCGAGCCCAG
    TGTGAAACCC TCTCCCCTGA TGGCATGCCT GAGGAACAGC CACAGACCAC CAAGCTGAAA
    GGCAAAATGC AGAGCAGGTT TGACGTGGCT GAGAATGTGG TAATCGTGGC ATCTCAGAAG
    CGGCCTCTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA
    TCTACCCAGA AGTTCTTCTT GAGGGTACTG CAGGTCATCA TCCAGCTCCG GGATGACACG
    CGCCGTGCTA ACAAGAAGGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA
    GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA
    ATGGTACGTG AGGGTCAGAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCAAGC
    CAGTCAGAGG CATCTGTCCG AAGGGAGGAG TCACCCATGG ATGTGGACCA GCCATCTCCC
    AGTGCTCAAG ATACTCAGTC CATTGGCTCA GATGGAACTC CACAGGGAGA GAAGGAGAAG
    GAGGAGAGGC CACCTGAGTT ACCCCTGCTC AGTGAGCAGC TGAACTTGGA TGAGCTGTGG
    GACATGCTGG GAGAGTGTCT AAAGGAACTG GAGGAATCCC ATGACCAGCA TGCGGTGCTA
    GTGCTACAAC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CAACAGAGCG GGAGAGCAAG
    CCTCCTGTCC GGGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCACCTCCA
    CTCTCCCCTG CCCCTTTAAC CCCAGCCACT CCTTCCTCCC TTGACCCGTT CTTCTCCCGG
    GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTTCTTCGT
    TTTGCAGAGA CTCATCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC CACCCACCTC
    GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT CGATGTCAAG
    CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTGGACGAGG GACTCCGGAA AGAAGACATG
    GCTGTGCACG TTCGTCGTGA TCATGTCTTT GAAGACTCCT ATCGTGAGCT GCACCGGAAA
    TCCCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT
    GCTGGAGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG
    TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGGGTCACAT ACACCATCAA TCCATCTTCT
    CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTTG GACGCATCGT GGCCAAAGCT
    GTGTATGACA ACCGCCTCCT GGAGTGCTAC TTTACTAGGT CCTTCTACAA ACACATTTTG
    GGCAAGTCTG TCAGATATAC AGATATGGAG AGTGAAGACT ACCACTTTTA CCAGGGCCTG
    GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ATCTCACTTT CAGCACTGAG
    GTCCAAGAGT TTGGAGTCTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG
    GTAACAGAGG AAAATAAGAA GGAATATGTA CACCTGGTGT GCCAGATGAG AATGACAGGA
    GCCATCCGCA AACAGCTGGC GGCTTTCTTA GAAGGTTTCT ATGAGATCAT TCCAAAGCGC
    CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTCA TATCAGGACT GCCCACAATC
    GACATTGACG ATTTGAAATC TAACACTGAA TATCACAAGT ACCAGTCCAA CTCTATTCAG
    ATCCAGTGGT TCTGGAGAGC ACTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC
    CAGTTTGTCA CAGGTACTTC CAAGGTGCCC CTGCAAGGTT TTGCTGCCCT TGAAGGCATG
    AATGGCATTC AGAAGTTTCA AATCCATCGA GATGACAGGT CCACAGACCG CCTGCCTTCA
    GCTCACACAT GTTTTAATCA GCTGGACCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC
    CACATGCTAC TATTGGCCAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35633
    Clone ID Related Accession (Same CDS sequence) XM_024987894.1 , XM_010821997.2 , XM_005228212.2 , XM_005228212.3 , XM_024987895.1
    Accession Version XM_005228212.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228212.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATAA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTCCACTT AGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGATTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT
    GTACTTGTGC GAAACTGTAT CCAGGCCATG ATTGACCCTT CCATGGATCC ATACCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CAAGCTACGA TGCTGGTGGC
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG CCGAAAAGAA TGCCCTTTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTGCACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TACAGATGTG
    ACTATGGAAA GTAGTCCCAG CTCATCCATC TCTATGGAGC ACCGGCTGGA TGTTGAATTA
    AGGGCATCAA GTTCCAGCAG CAGTAGCATC TCCTCTGGCC CTGGCCCTCG TCCTGGAGTC
    CAGTGTATTC CACAACGAGC CGCACTTCTC AAATCCATGT TGAATTTCCT CAAGAAGGCC
    ATTCAAGACC CTGCTTTCTC AGATGGCATA CGACATGTGA TGGATGGTTC TCTGCCTACC
    TCCCTGAAAC ACATCATCAG CAATGCAGAA TACTATGGCC CATCACTCTT TCTCCTAGCT
    ACTGAAGTGG TGACTGTGTT TGTATTTCAA GAACCATCAT TGCTTTCCTC ACTCCAGGAC
    AATGGATTGA CAGATGTCAT GCTACATGCA CTGCTTATCA AAGACGTTCC TGCTACCCGT
    GAAGTTCTTG GCTCCCTCCC AAATGTTTTC AGTGCACTCT GCTTGAATGC CCGAGGTCTG
    CAGTCATTTG TACAGTGTCA GCCTTTTGAA CGTCTCTTCA AAGTTCTCCT GTCTCCAGAT
    TACCTCCCAG CCATGCGGAG AAGGAGGAGT TCTGATCCTC TAGGGGATAC TGCCTCCAAC
    CTAGGAAGTG CTGTAGATGA GCTCATGAGA CATCAGCCTA CCCTCAAAAC AGATGCAACG
    ACTGCGATCA TCAAGTTACT TGAAGAAATC TGTAATCTTG GAAGAGACCC TAAATACATC
    TGTCAGAAGC CATCAATCCA GAAGGCAGAT GGCACTGCCG CTGCTCCTCC CCCAAGATCT
    AACCATGCTG CAGAAGAGGC CTCTAGTGAG GATGAGGAGG AAGAGGAAGT ACAGGCCATG
    CAGAGCTTTA ATTCTACCCA GCAGAATGAA ACTGAGCCTA ATCAGCAGGT TGTTGGTACA
    GAGGAGCGTA TTCCTATTCC CCTCATGGAT TACATCCTTA ATGTGATGAA ATTTGTGGAA
    TCTATTCTGA GCAACAATAC AACTGATGAC CACTGCCAGG AATTTGTGAA TCAGAAAGGA
    CTCTTGCCTT TGGTCACCAT TTTGGGTCTT CCCAATCTGC CCATTGACTT TCCCACGTCT
    GCAGCCTGTC AGGCTGTTGC AGGTGTCTGC AAATCCATAT TGACACTGTC ACATGAACCC
    AAAGTCCTCC AGGAAGGTCT CCTTCAGTTG GACTCCATTC TTTCCTCCTT GGAGCCCTTA
    CACCGCCCAA TAGAATCCCC CGGGGGCTCA GTGTTGTTGC GAGAACTGGC TTGTGCTGGC
    AATGTTGCTG ATGCTACCCT CTCAGCTCAA GCCACACCTC TACTACATGC ACTCACTGCT
    GCCCATGCTT ACATCATGAT GTTTGTTCAT ACTTGCAGGG TTGGACAGAG TGAAATTCGT
    TCCATCTCCA TAAACCAGTG GGGCTCTCAG TTGGGTCTGA GTGTTCTGAG CAAGCTGAGC
    CAGTTATACT GTTCCCTGGT ATGGGAAAGC ACTGTCCTCC TCTCCCTCTG TACCCCAAAC
    AGCTTGCCAT CTGGGTGTGA ATTTGGCCAG GCAGACATGC AGAAACTGGT TCCAAAGGAT
    GAGAAGACAG GTACGACCCA GGGCGGAAAA AGATCAGATG GGGATCAGGA TGGAACCACT
    GGAAGTATGG ATGCTTCTAC CCAGGGCTTG TTGGAAGGCA TTGGGCTTGA TGGTGACACA
    CTGGCTCCTA TGGAGACAGA CGAACCTACT GCTTCAGACT CTAAGGGCAA ATCTAAAATC
    ACACCTGCCA TGGCTGCCAG AATCAAGCAA ATCAAGCCAT TGTTGTCAGC TTCCTCTAGA
    TTAGGCAGAG CACTGGCTGA GCTGTTTGGA CTCCTTGTTA AACTTTGTGT GGGGTCTCCT
    GTCCGCCAGA GAAGAAGCCA TCATGCTGCC AGCACCACCA CAGCACCAAC ACCTGCTGCT
    CGATCAACGG CCTCAGCCCT CACTAAGCTC CTCACAAAAG GCTTATCATG GCAGCCTCCA
    CCATATACGC CTACTCCACG CTTCAGGCTG ACCTTCTTCA TCTGTTCAGT TGGTTTTACA
    TCCCCAATGC TGTTTGATGA GAGGAAGTAT CCCTACCACC TCATGCTGCA GAAATTTCTC
    TGCTCTGGAG GCCACAATGC CCTTTTTGAA ACATTCAACT GGGCTCTGTC TATGGGAGGA
    AAAGTTCCTG TTGCTGAGGG ATTAGAACAT TCAGACTTAC CTGATGGCAC AGGAGAATTC
    CTAGATGCCT GGCTAATGCT GGTGGAGAAG ATGGTAAATC CCACCACTGT GCTTGAATCT
    CCACATTCAC TGCCTGCCAA ATTGCCTGGA GGTGTTCAGA ACTTTCCACA GTTCAGTGCC
    CTCCGCTTCC TTGTGGTAAC TCAGAAGGCA GCATTTACTT GCATCAAGAA CCTGTGGAAC
    CGGAAACCCT TGAAAGTGTA TGGTGGGCGA ATGGCTGAGT CCATGCTAGC CATCCTGTGC
    CACATCCTCC GAGGAGAACC TGTGATTCGA GAGAGACTGA GCAAAGAAAA GGAGGGATCT
    CGAGGAGAAG AGGATACAGG ACAAGAAGAA GGTGGCTCTC GCCGGGAACC TCAAGTCAAC
    CAGCAACAAC TACAACAGCT CATGGACATG GGCTTCACAA GGGAACATGC CATGGAGGCC
    CTGTTGAACA CCAGTACCAT GGAGCAGGCC ACAGAGTACC TTCTAACCCA CCCTCCCCCG
    ATCATGGGAG GAGTTGTTCG GGATCTGAGT ATGTCTGAAG AGGACCAGAT GATGAGAGCG
    ATTGCCATGT CTCTGGGACA GGATATCCCA ATGGATCAAA GAGCAGAGTC ACCTGAGGAA
    GTTGCTTGCC GAAAGGAAGA GGAGGAACGG AAAGCTCGAG AAAAGCAGGA AGAGGAAGAG
    GCTAAATGTC TAGAGAAGTT CCAGGATGCT GATCCATTGG AGCAAGATGA GCTCCACACT
    TTCACAGATA CAATGTTGCC AGGCTGCTTT CACCTTCTTG ATGAGTTGCC AGACACTGTA
    TACCGAGTGT GTGATCTGAT CATGACAGCA ATCAAACGAA ATGGAGCAGA CTATCGAGAC
    ATGATTCTGA AGCAAGTAGT TAATCAGGTG TGGGAAGCTG CTGATGTATT GATCAAAGCT
    GCTCTTCCCC TGACAACAAG TGACACAAAA ACTGTATCAG AGTGGATCAG TCAGATGGCC
    ACTCTACCCC AGGCCTCTAA TTTGGCCACT AGAATCTTGC TTTTAACACT GCTTTTTGAG
    GAATTGAAGC TACCTTGTGC TTGGGTGGTT GAATCTAGTG GCATCCTTAA TGTCCTAATC
    AAACTCTTGG AAGTGGTTCA GCCCTGCCTA CAGGCCGCCA AGGAACAAAA GGAGGTCCAG
    ACCCCAAAGT GGATCACTCC AGTGTTGCTC CTGATTGATT TCTATGAAAA AACAGCCATT
    TCCTCAAAGA GGAGAGCCCA AATGACTAAG TATCTACAGT CTAACAACAA CAACTGGCGC
    TGGTTTGATG ACCGCTCTGG ACGTTGGTGT AGTTACAGTG CAAGCAACAA CAGCACTATT
    GATTCTGCCT GGAAATCTGG AGAGACAAGT GTGCGTTTCA CTGCAGGCCG AAGAAGATAC
    ACTGTCCAGT TCACTACAAT GGTACAGGTT AATGAGGAAA CAGGGAACCG GCGTCCTGTG
    ATGCTGACAC TCCTGAGGGT ACCCCGATTG AATAAAAATT CTAAAAACAG CAATGGACAG
    GAACTAGAGA AGACACTGGA AGAAAGCAAA GAAATGGATA TCAAACGTAA AGAAAATAAA
    ACCAGTGATA CCCCCTTGGC CCTAGACAGT ACAAATACTG AAAAGGAGAC AAGCCTGGAA
    GAAACAAAAA TTGGGGAGAT CTTGATCCAG GGCCTGACAG AGGATATGGT GACTGTTTTA
    ATCCGGGCCT GTGTGAGCAT GCTAGGCGTC CCTGTGGATC CAGACACTTT GCATGCCACC
    CTTCGCCTAT GCCTGAGGCT CACCCGAGAC CACAAATATG CCATGATGTT TGCAGAGCTG
    AAGAGTACTC GCATGATCTT AAATTTGACC CAGAGTTCAG GCTTCAATGG GTTTACTCCC
    CTGGTTACCC TTCTCTTAAG ACACATCATT GAGGACCCCT GCACCCTGCG CCATACCATG
    GAAAAGGTTG TTCGCTCAGC AGCTACAAGT GGAGCTGGTA GCACTACTTC TGGTGTTGTA
    TCTGGCAGCC TTGGCTCTCG GGAGATCAAC TACATCCTTC GTGTCCTTGG ACCAGCCGCA
    TGCCGCAACC CAGACATTTT CACAGAAGTG GCCAACTGCT GTATCCGCAT TGCCCTTCCA
    GCCCCTCGCG GCTCAGGAAC TGCTTCAGAT GATGAGTTTG AGAATCTCAG AATTAAAGGC
    CCGAATGCTG TACAGCTGGT GAAAACAACC CCTTTGAAGC CCTCACCTCT GCCTGTCATT
    CCTGATACTA TCAAGGAAGT GATCTATGAC ATGCTAAATG CCCTGGCTGC ATACCATGCT
    CCAGAGGAAG CAGACAAATC TGATCCTAAA CCAGGGGGTA TGACTCAAGA GGTTGGCCAG
    CTCCTGCAAG ACATGGGTGA TGACGTATAC CAGCAGTACC GGTCACTTAC CCGTCAGAGC
    AGTGACTTTG ATGCACAGAC AGGATTTTCC ATTAATAGTC AGGTCTTTGC TGCAGATGGT
    TCCTCCACTG AGACTTCCGC ATCTGGGGTA TCCCAAGGAG AAGTGTCAAC TCCGGAGGAG
    TCTCGGGATG GGAAGAAAGA TAAAGAAGGG GACCGGGCCT CTGAGGAAGG CAAGCAGAAA
    GGCAAGGGCA GCAAACCTTT AATGCCCACC TCCACTATTC TTCGGCTTCT GGCAGAGTTG
    GTGAGATCCT ATGTTGGTAT TGCTACCCTG ATTGCCAACT ACAGCTACAC TGTGGGCCAG
    TCTGAGCTGA TCAAAGAGGA CTGCAGTGTG CTAGCTTTTG TCTTGGACCA CCTCCTCCCG
    CATACCCAGA ATGCAGAAGA CAAAGACACA CCGGCGCTGG CCCGCCTGTT CCTCGCAAGC
    CTGGCTGCCT CAGGGAGTGG CACAGATGCC CAGGTGGCCC TTGTGAATGA AGTAAAAGCA
    GCCCTGGGAA GGGCACTGGC TATGGCTGAG AGTACAGAGA AACATGCCAG ACTTCAGGCA
    GTGATGTGTA TCATCAGTAC TATCATGGAG TCCTGCCCCT CCACCTCCAG TTTCTACAGC
    AGTGCAACAG CCAAGACCCA ACACAACGGC ATGAACAACA TCATTCGACT CTTCCTGAAG
    AAGGGACTGG TTAATGACCT GGCCAGAGTG CCTCACAGCC TGGACCTGTC CAGTCCCAAC
    ATGGCCAACA CTGTCAATGC TGCTCTGAAG CCTTTGGAAA CACTTTCCCG GATTGTGAAC
    CAGCCCAGTA GCCTTTTTGG CAGTAAGAGT GCTTCTAGCA AGAGCAAGTC TGAGCAGGAT
    GCCCAAGGAG CTGCTCAGGA CTCTAATAGC AACCAACAGG ACCCAGGCGA GCCTGGGGAA
    ACAGAAGTGC AGGAGGAGGA TCATGATGTC ACTCAGACAG AGGTGGCAGA TGGGGATATC
    ATGGATGGGG AGGCTGAAAC CGACTCAGTG GTGATTGCTG GGCAGCCTGA GGTGCTCAGT
    TCACAAGAGA TGCAGGTTGA GAATGAGCTG GAGGACCTGA TAGATGAGTT GCTTGAGAGG
    GATGGCGGAT CTGGGAACAG TACAATTATA GTGAGCAGAA GTGGAGAGGA TGAATCACAA
    GAGGACGTGC TGATGGATGA AGCTCCTTCC AACCTCAGCC AAGCTTCCAC CTTGCAGGCC
    AACCGAGAAG ATTCCATGAA TATCCTGGAC CCTGAGGATG AGGAGGAGCA CACTCAGGAA
    GAGGACAGCA GTGGCAGTAA CGAGGATGAG GATGATAGTC AGGATGAAGA GGAGGAGGAG
    GAGGAAGATG AGGAAGATGA TCAGGAGGAT GATGAAGGTG AAGAGGGAGA TGAAGACGAT
    GACGACGATG GCTCTGAAAT GGAATTGGAT GAGGATTACC CTGATATGAA CGCTTCTCCC
    TTGGTCCGAT TTGAGCGCTT TGACCGGGAG GATGATCTCA TCATTGAGTT TGACAACATG
    TTCTCCAGTG CTACAGACAT CCCCCCATCC CCAGGAAATA TCCCTACCAC CCACCCACTG
    ATGGTACGCC ATGCAGATCA CAGTTCCCTG ACACTGGGCA GTGGCTCCTC TACAACACGT
    CTCACCCAGG GCATTGGGCG CAGTCAAAGG ACCCTGAGAC AGCTGACAGC CAACACAGGC
    CATACCATTC ACGTTCACTA CCCTGGGAAT CGCCAGCCCA ATCCTCCTCT TATACTCCAG
    AGGTTGCTTG GTCCCTCTGC TGCTGCTGAC ATCCTTCAGC TGAGCAGCAG CCTTCCCCTA
    CAAAGCCGAG GCCGAGCCCG CCTCCTAGTG GGCAACGATG ACGTCCACAT CATCGCCCGG
    TCTGATGATG AACTACTGGA TGACTTTTTC CATGATCAGA GCACAGCTAC CAGCCAAGCA
    GGGACCCTAT CCAGTATCCC TACAGCCCTG ACTCGCTGGA CAGAGGAATG CAAAGTTCTT
    GATGCTGAGA GCATGCATGA CTGTGTTTCA GTTGTTAAAG TGCCCATTGT CAATCATCTG
    GAATTCCTGA GGGATGAGGA GCTGGAAGAA AGGAGGGAGA AAAGGAGGAA ACAACTAGCT
    GAGGAAGAAA CAAAGATAAC CGACAAAGGC AAAGAAGATA AGGAGAACAG GGATCAGAGT
    GCACAGTGTG CTGCATCTAA GACAAATGAC TCCACTGAAC AGAACCTCTC AGATGGGACT
    CCTATGCCTG ACAGCTACCC AGCAACCCCA TCTTCAACTG ATGTAGCTAC ATCTGAGCCC
    AGGGAGACCC TTGTCACTCT GCAGCCCTCC CAACAGCAAC CAACACTCCC AACCCCACCA
    GCCTTGGGAG AGATTCCTCA AGAGCTGCAG TCCCCAATTG GAGAAGGTGG GAGCTCTACA
    CAGCTATTGA TGCCTGTAGA GCCAGAGGAA TTGGGTCCCA CAAGGCCAAG TGGAGAAGCA
    GAAACAACTC AGATGGAGCT ATCTCCAGCT CCCACTATAA CCTCTCTTTC CCCAGAGAGA
    GCTGAAGATT CTGATGCTCT GACAGCTGTC AGCAGTCAAC TGGAAGGCTC TCCCATGGAT
    ACTAGCAGCC TGGCTTCCTG TACCCTGGAG GAGGCTGTGG GTGACGCCCC AGCAACTGGC
    AGTTCTGAGC AGCCCACAGC AGGCAGCTCC ACTCCTGGGG ATGCCCCACC AGTTGTGACA
    GAAGTACAAG GCAGGGGTGA TGGGTCAGGG GAACCTACCC AGCCCCCTGA GGACAGCTCT
    CCCCCTGCAT CCTCTGAGAG TTCTTCCACC AGAGATTCCG CGGTGGCCAT TTCTGGAGCA
    GATTCCCGGG GAATCCTGGA AGAGCCATTG CCTTCAACAA GCAGTGAAGA AGAAGATCCT
    CTTGCAGGTA TTAGTCTCCC CGAAGGTGTG GACCCCTCTT TTCTGGCCGC TCTGCCTGAT
    GACATTCGCC GAGAAGTCCT ACAGAACCAG CTGGGCATTC GTCCACCAAC ACGAACTGCC
    CCTTCAACAA ATAACTCAGC TCCTGCAGTG GTGGGGAATC CTGGTGTCAC CGAAGTGAGC
    CCTGAGTTCC TGGCTGCTCT GCCTCCAGCC ATCCAGGAGG AAGTACTGGC ACAGCAGAGA
    GCTGAGCAAC AGCGACGGGA ACTAGCACAA AATGCCAGCT CAGACACCCC CATGGACCCT
    GTGACCTTCA TCCAGACTCT GCCCTCAGAC CTGCGCCGTA GTGTCCTAGA GGACATGGAG
    GACAGTGTGT TGGCCGTGAT GCCACCTGAC ATCGCAGCTG AAGCTCAAGC CCTGAGGCGA
    GAGCAAGAAG CCCGGCAGAG GCAGCTCATG CATGAGCGAC TTTTTGGGCA CAGCAGCACC
    TCTGCACTCT CTGCCATTCT CCGAAGCCCA GCCTTCACCA GTCGCCTGAG TGGCAACCGT
    GGGGTCCAAT ACACTCGCCT TGCTGTGCAA AGAGGTGGCA CCTTCCAGAT GGGGGGTAGC
    AGCAGCCATA ATAGGCCTTC TGGCAGTAAT GTGGACACCC TCCTACGTCT TCGAGGACGG
    CTCCTCCTGG ATCACGAAGC CCTGTCTTGT CTCCTGGTCC TACTTTTTGT GGATGAGCCA
    AAACTCAATA CTAGCCGTCT TCACCGAGTA CTGAGAAATC TCTGCTACCA TGCCCAGACC
    CGCCACTGGG TTATCCGTAG CCTGCTTTCC ATCTTGCAGC GAAGCAGTGA GAGTGAGCTT
    TGCATTGAAA CACCCAAGCT CTCCTCAAGT GAGGAAAAGG GCAAAAAGTC AAGCAAGAGC
    TGTGGGTCAA GCAGCCATGA GAACCGGCCC CTGGACCTGC TACACAAGAT GGAGTCTAAG
    AGTTCCAACC AGCTTTCCTG GCTCTCAGTA TCCATGGATG CAGCCCTAGG CTGCAGGACT
    AATATATTCC AGATCCAGCG TTCAGGGGGT CGCAAACATA CTGAGAAACA TGCGAGTAGT
    GGCTCCACTG TCCATATCCA CCCCCAGGCT GCTCCTGTTG TCTGCAGACA TGTTCTGGAT
    ACACTCATTC AATTGGCCAA GGTGTTTCCC AGTCACTTCA CACAGCAGCG GACCAAAGAA
    ACAAACTGTG AGAGTGATCG GGAGAGGGGC AGTAAGGCCT GCAGCCCATG CTCTTCACAG
    TCCACAAGCA GTGGCATTTG CACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG
    AATGTCAGCC GGAAAGGCAA GAACTCCGTG AAGTCAGTGC CAGTGAGCGC TGGTGGTGAG
    GGGGAAACCT CCCCATACAG CCTCGAAGCT TCTCCACTGG GGCAACTCAT GAACATGTTG
    TCACACCCAG TCATCCGCCG GAGCTCTCTC TTAACTGAGA AGCTCCTCAG ACTTCTTTCT
    CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTGTCGGAAG CACAGGCTAA TTCTGGCAGC
    AGTGCTTCCT CCACCACTGT TGCCACTTCA ACCACATCTA CCACCACTAT CAGTGCCACC
    TCCAGCACAT CTACTCTGCC TGCTGCAACC ACCCCTGTCA CTTCTGTTCC AGCCCTGGTT
    GCTGCCACGG CTATATCCAC CATTGCCGTA GCCGCTTCAA CCACAGTGAC TACCCCCACA
    ACTGCTACCA CTACTGTTTC AACTGCTACT ACTACTAAGG CCAGCAAATC TCCAGCTAAA
    GTTGGTGATA TCGGCAGCAG CAGCAGCACA GACTTTAAGA TGGTATCCTC TGGCCTCACT
    GAAAATCAGC TCCAGCTCTC TGTGGAAGTG TTGACATCCC ATTCTTGTTC TGAAGAAGGC
    CTAGAAGATG CAGCAAATGT GTTACTGCAG CTCTCTCGGG GGGACCCTGG GACCCGGGAC
    ACTGTTTTGA AGTTGCTACT GAATGGAGCC CGCCATCTGG GTTATACGCT TTGTAAACAG
    ATAGGAACTC TTCTGGCTGA GCTACGGGAA TACAATCTGG AACAGCAGCG GCGAGCCCAG
    TGTGAAACCC TCTCCCCTGA TGGCATGCCT GAGGAACAGC CACAGACCAC CAAGCTGAAA
    GGCAAAATGC AGAGCAGGTT TGACGTGGCT GAGAATGTGG TAATCGTGGC ATCTCAGAAG
    CGGCCTCTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA
    TCTACCCAGA AGTTCTTCTT GAGGGTACTG CAGGTCATCA TCCAGCTCCG GGATGACACG
    CGCCGTGCTA ACAAGAAGGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA
    GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA
    ATGGTACGTG AGGGTCAGAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCAAGC
    CAGTCAGAGG CATCTGTCCG AAGGGAGGAG TCACCCATGG ATGTGGACCA GCCATCTCCC
    AGTGCTCAAG ATACTCAGTC CATTGGCTCA GATGGAACTC CACAGGGAGA GAAGGAGAAG
    GAGGAGAGGC CACCTGAGTT ACCCCTGCTC AGTGAGCAGC TGAACTTGGA TGAGCTGTGG
    GACATGCTGG GAGAGTGTCT AAAGGAACTG GAGGAATCCC ATGACCAGCA TGCGGTGCTA
    GTGCTACAAC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CAACAGAGCG GGAGAGCAAG
    CCTCCTGTCC GGGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCACCTCCA
    CTCTCCCCTG CCCCTTTAAC CCCAGCCACT CCTTCCTCCC TTGACCCGTT CTTCTCCCGG
    GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTTCTTCGT
    TTTGCAGAGA CTCATCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC CACCCACCTC
    GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT CGATGTCAAG
    CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTGGACGAGG GACTCCGGAA AGAAGACATG
    GCTGTGCACG TTCGTCGTGA TCATGTCTTT GAAGACTCCT ATCGTGAGCT GCACCGGAAA
    TCCCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT
    GCTGGAGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG
    TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGGGTCACAT ACACCATCAA TCCATCTTCT
    CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTTG GACGCATCGT GGCCAAAGCT
    GTGTATGACA ACCGCCTCCT GGAGTGCTAC TTTACTAGGT CCTTCTACAA ACACATTTTG
    GGCAAGTCTG TCAGATATAC AGATATGGAG AGTGAAGACT ACCACTTTTA CCAGGGCCTG
    GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ATCTCACTTT CAGCACTGAG
    GTCCAAGAGT TTGGAGTCTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG
    GTAACAGAGG AAAATAAGAA GGAATATGTA CACCTGGTGT GCCAGATGAG AATGACAGGA
    GCCATCCGCA AACAGCTGGC GGCTTTCTTA GAAGGTTTCT ATGAGATCAT TCCAAAGCGC
    CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTCA TATCAGGACT GCCCACAATC
    GACATTGACG ATTTGAAATC TAACACTGAA TATCACAAGT ACCAGTCCAA CTCTATTCAG
    ATCCAGTGGT TCTGGAGAGC ACTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC
    CAGTTTGTCA CAGGTACTTC CAAGGTGCCC CTGCAAGGTT TTGCTGCCCT TGAAGGCATG
    AATGGCATTC AGAAGTTTCA AATCCATCGA GATGACAGGT CCACAGACCG CCTGCCTTCA
    GCTCACACAT GTTTTAATCA GCTGGACCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC
    CACATGCTAC TATTGGCCAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228269.1
    CDS370..13623
    Translation

    Target ORF information:

    RefSeq Version XM_005228212.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228212.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATAA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTCCACTT AGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGATTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT
    GTACTTGTGC GAAACTGTAT CCAGGCCATG ATTGACCCTT CCATGGATCC ATACCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CAAGCTACGA TGCTGGTGGC
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG CCGAAAAGAA TGCCCTTTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTGCACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TACAGATGTG
    ACTATGGAAA GTAGTCCCAG CTCATCCATC TCTATGGAGC ACCGGCTGGA TGTTGAATTA
    AGGGCATCAA GTTCCAGCAG CAGTAGCATC TCCTCTGGCC CTGGCCCTCG TCCTGGAGTC
    CAGTGTATTC CACAACGAGC CGCACTTCTC AAATCCATGT TGAATTTCCT CAAGAAGGCC
    ATTCAAGACC CTGCTTTCTC AGATGGCATA CGACATGTGA TGGATGGTTC TCTGCCTACC
    TCCCTGAAAC ACATCATCAG CAATGCAGAA TACTATGGCC CATCACTCTT TCTCCTAGCT
    ACTGAAGTGG TGACTGTGTT TGTATTTCAA GAACCATCAT TGCTTTCCTC ACTCCAGGAC
    AATGGATTGA CAGATGTCAT GCTACATGCA CTGCTTATCA AAGACGTTCC TGCTACCCGT
    GAAGTTCTTG GCTCCCTCCC AAATGTTTTC AGTGCACTCT GCTTGAATGC CCGAGGTCTG
    CAGTCATTTG TACAGTGTCA GCCTTTTGAA CGTCTCTTCA AAGTTCTCCT GTCTCCAGAT
    TACCTCCCAG CCATGCGGAG AAGGAGGAGT TCTGATCCTC TAGGGGATAC TGCCTCCAAC
    CTAGGAAGTG CTGTAGATGA GCTCATGAGA CATCAGCCTA CCCTCAAAAC AGATGCAACG
    ACTGCGATCA TCAAGTTACT TGAAGAAATC TGTAATCTTG GAAGAGACCC TAAATACATC
    TGTCAGAAGC CATCAATCCA GAAGGCAGAT GGCACTGCCG CTGCTCCTCC CCCAAGATCT
    AACCATGCTG CAGAAGAGGC CTCTAGTGAG GATGAGGAGG AAGAGGAAGT ACAGGCCATG
    CAGAGCTTTA ATTCTACCCA GCAGAATGAA ACTGAGCCTA ATCAGCAGGT TGTTGGTACA
    GAGGAGCGTA TTCCTATTCC CCTCATGGAT TACATCCTTA ATGTGATGAA ATTTGTGGAA
    TCTATTCTGA GCAACAATAC AACTGATGAC CACTGCCAGG AATTTGTGAA TCAGAAAGGA
    CTCTTGCCTT TGGTCACCAT TTTGGGTCTT CCCAATCTGC CCATTGACTT TCCCACGTCT
    GCAGCCTGTC AGGCTGTTGC AGGTGTCTGC AAATCCATAT TGACACTGTC ACATGAACCC
    AAAGTCCTCC AGGAAGGTCT CCTTCAGTTG GACTCCATTC TTTCCTCCTT GGAGCCCTTA
    CACCGCCCAA TAGAATCCCC CGGGGGCTCA GTGTTGTTGC GAGAACTGGC TTGTGCTGGC
    AATGTTGCTG ATGCTACCCT CTCAGCTCAA GCCACACCTC TACTACATGC ACTCACTGCT
    GCCCATGCTT ACATCATGAT GTTTGTTCAT ACTTGCAGGG TTGGACAGAG TGAAATTCGT
    TCCATCTCCA TAAACCAGTG GGGCTCTCAG TTGGGTCTGA GTGTTCTGAG CAAGCTGAGC
    CAGTTATACT GTTCCCTGGT ATGGGAAAGC ACTGTCCTCC TCTCCCTCTG TACCCCAAAC
    AGCTTGCCAT CTGGGTGTGA ATTTGGCCAG GCAGACATGC AGAAACTGGT TCCAAAGGAT
    GAGAAGACAG GTACGACCCA GGGCGGAAAA AGATCAGATG GGGATCAGGA TGGAACCACT
    GGAAGTATGG ATGCTTCTAC CCAGGGCTTG TTGGAAGGCA TTGGGCTTGA TGGTGACACA
    CTGGCTCCTA TGGAGACAGA CGAACCTACT GCTTCAGACT CTAAGGGCAA ATCTAAAATC
    ACACCTGCCA TGGCTGCCAG AATCAAGCAA ATCAAGCCAT TGTTGTCAGC TTCCTCTAGA
    TTAGGCAGAG CACTGGCTGA GCTGTTTGGA CTCCTTGTTA AACTTTGTGT GGGGTCTCCT
    GTCCGCCAGA GAAGAAGCCA TCATGCTGCC AGCACCACCA CAGCACCAAC ACCTGCTGCT
    CGATCAACGG CCTCAGCCCT CACTAAGCTC CTCACAAAAG GCTTATCATG GCAGCCTCCA
    CCATATACGC CTACTCCACG CTTCAGGCTG ACCTTCTTCA TCTGTTCAGT TGGTTTTACA
    TCCCCAATGC TGTTTGATGA GAGGAAGTAT CCCTACCACC TCATGCTGCA GAAATTTCTC
    TGCTCTGGAG GCCACAATGC CCTTTTTGAA ACATTCAACT GGGCTCTGTC TATGGGAGGA
    AAAGTTCCTG TTGCTGAGGG ATTAGAACAT TCAGACTTAC CTGATGGCAC AGGAGAATTC
    CTAGATGCCT GGCTAATGCT GGTGGAGAAG ATGGTAAATC CCACCACTGT GCTTGAATCT
    CCACATTCAC TGCCTGCCAA ATTGCCTGGA GGTGTTCAGA ACTTTCCACA GTTCAGTGCC
    CTCCGCTTCC TTGTGGTAAC TCAGAAGGCA GCATTTACTT GCATCAAGAA CCTGTGGAAC
    CGGAAACCCT TGAAAGTGTA TGGTGGGCGA ATGGCTGAGT CCATGCTAGC CATCCTGTGC
    CACATCCTCC GAGGAGAACC TGTGATTCGA GAGAGACTGA GCAAAGAAAA GGAGGGATCT
    CGAGGAGAAG AGGATACAGG ACAAGAAGAA GGTGGCTCTC GCCGGGAACC TCAAGTCAAC
    CAGCAACAAC TACAACAGCT CATGGACATG GGCTTCACAA GGGAACATGC CATGGAGGCC
    CTGTTGAACA CCAGTACCAT GGAGCAGGCC ACAGAGTACC TTCTAACCCA CCCTCCCCCG
    ATCATGGGAG GAGTTGTTCG GGATCTGAGT ATGTCTGAAG AGGACCAGAT GATGAGAGCG
    ATTGCCATGT CTCTGGGACA GGATATCCCA ATGGATCAAA GAGCAGAGTC ACCTGAGGAA
    GTTGCTTGCC GAAAGGAAGA GGAGGAACGG AAAGCTCGAG AAAAGCAGGA AGAGGAAGAG
    GCTAAATGTC TAGAGAAGTT CCAGGATGCT GATCCATTGG AGCAAGATGA GCTCCACACT
    TTCACAGATA CAATGTTGCC AGGCTGCTTT CACCTTCTTG ATGAGTTGCC AGACACTGTA
    TACCGAGTGT GTGATCTGAT CATGACAGCA ATCAAACGAA ATGGAGCAGA CTATCGAGAC
    ATGATTCTGA AGCAAGTAGT TAATCAGGTG TGGGAAGCTG CTGATGTATT GATCAAAGCT
    GCTCTTCCCC TGACAACAAG TGACACAAAA ACTGTATCAG AGTGGATCAG TCAGATGGCC
    ACTCTACCCC AGGCCTCTAA TTTGGCCACT AGAATCTTGC TTTTAACACT GCTTTTTGAG
    GAATTGAAGC TACCTTGTGC TTGGGTGGTT GAATCTAGTG GCATCCTTAA TGTCCTAATC
    AAACTCTTGG AAGTGGTTCA GCCCTGCCTA CAGGCCGCCA AGGAACAAAA GGAGGTCCAG
    ACCCCAAAGT GGATCACTCC AGTGTTGCTC CTGATTGATT TCTATGAAAA AACAGCCATT
    TCCTCAAAGA GGAGAGCCCA AATGACTAAG TATCTACAGT CTAACAACAA CAACTGGCGC
    TGGTTTGATG ACCGCTCTGG ACGTTGGTGT AGTTACAGTG CAAGCAACAA CAGCACTATT
    GATTCTGCCT GGAAATCTGG AGAGACAAGT GTGCGTTTCA CTGCAGGCCG AAGAAGATAC
    ACTGTCCAGT TCACTACAAT GGTACAGGTT AATGAGGAAA CAGGGAACCG GCGTCCTGTG
    ATGCTGACAC TCCTGAGGGT ACCCCGATTG AATAAAAATT CTAAAAACAG CAATGGACAG
    GAACTAGAGA AGACACTGGA AGAAAGCAAA GAAATGGATA TCAAACGTAA AGAAAATAAA
    ACCAGTGATA CCCCCTTGGC CCTAGACAGT ACAAATACTG AAAAGGAGAC AAGCCTGGAA
    GAAACAAAAA TTGGGGAGAT CTTGATCCAG GGCCTGACAG AGGATATGGT GACTGTTTTA
    ATCCGGGCCT GTGTGAGCAT GCTAGGCGTC CCTGTGGATC CAGACACTTT GCATGCCACC
    CTTCGCCTAT GCCTGAGGCT CACCCGAGAC CACAAATATG CCATGATGTT TGCAGAGCTG
    AAGAGTACTC GCATGATCTT AAATTTGACC CAGAGTTCAG GCTTCAATGG GTTTACTCCC
    CTGGTTACCC TTCTCTTAAG ACACATCATT GAGGACCCCT GCACCCTGCG CCATACCATG
    GAAAAGGTTG TTCGCTCAGC AGCTACAAGT GGAGCTGGTA GCACTACTTC TGGTGTTGTA
    TCTGGCAGCC TTGGCTCTCG GGAGATCAAC TACATCCTTC GTGTCCTTGG ACCAGCCGCA
    TGCCGCAACC CAGACATTTT CACAGAAGTG GCCAACTGCT GTATCCGCAT TGCCCTTCCA
    GCCCCTCGCG GCTCAGGAAC TGCTTCAGAT GATGAGTTTG AGAATCTCAG AATTAAAGGC
    CCGAATGCTG TACAGCTGGT GAAAACAACC CCTTTGAAGC CCTCACCTCT GCCTGTCATT
    CCTGATACTA TCAAGGAAGT GATCTATGAC ATGCTAAATG CCCTGGCTGC ATACCATGCT
    CCAGAGGAAG CAGACAAATC TGATCCTAAA CCAGGGGGTA TGACTCAAGA GGTTGGCCAG
    CTCCTGCAAG ACATGGGTGA TGACGTATAC CAGCAGTACC GGTCACTTAC CCGTCAGAGC
    AGTGACTTTG ATGCACAGAC AGGATTTTCC ATTAATAGTC AGGTCTTTGC TGCAGATGGT
    TCCTCCACTG AGACTTCCGC ATCTGGGGTA TCCCAAGGAG AAGTGTCAAC TCCGGAGGAG
    TCTCGGGATG GGAAGAAAGA TAAAGAAGGG GACCGGGCCT CTGAGGAAGG CAAGCAGAAA
    GGCAAGGGCA GCAAACCTTT AATGCCCACC TCCACTATTC TTCGGCTTCT GGCAGAGTTG
    GTGAGATCCT ATGTTGGTAT TGCTACCCTG ATTGCCAACT ACAGCTACAC TGTGGGCCAG
    TCTGAGCTGA TCAAAGAGGA CTGCAGTGTG CTAGCTTTTG TCTTGGACCA CCTCCTCCCG
    CATACCCAGA ATGCAGAAGA CAAAGACACA CCGGCGCTGG CCCGCCTGTT CCTCGCAAGC
    CTGGCTGCCT CAGGGAGTGG CACAGATGCC CAGGTGGCCC TTGTGAATGA AGTAAAAGCA
    GCCCTGGGAA GGGCACTGGC TATGGCTGAG AGTACAGAGA AACATGCCAG ACTTCAGGCA
    GTGATGTGTA TCATCAGTAC TATCATGGAG TCCTGCCCCT CCACCTCCAG TTTCTACAGC
    AGTGCAACAG CCAAGACCCA ACACAACGGC ATGAACAACA TCATTCGACT CTTCCTGAAG
    AAGGGACTGG TTAATGACCT GGCCAGAGTG CCTCACAGCC TGGACCTGTC CAGTCCCAAC
    ATGGCCAACA CTGTCAATGC TGCTCTGAAG CCTTTGGAAA CACTTTCCCG GATTGTGAAC
    CAGCCCAGTA GCCTTTTTGG CAGTAAGAGT GCTTCTAGCA AGAGCAAGTC TGAGCAGGAT
    GCCCAAGGAG CTGCTCAGGA CTCTAATAGC AACCAACAGG ACCCAGGCGA GCCTGGGGAA
    ACAGAAGTGC AGGAGGAGGA TCATGATGTC ACTCAGACAG AGGTGGCAGA TGGGGATATC
    ATGGATGGGG AGGCTGAAAC CGACTCAGTG GTGATTGCTG GGCAGCCTGA GGTGCTCAGT
    TCACAAGAGA TGCAGGTTGA GAATGAGCTG GAGGACCTGA TAGATGAGTT GCTTGAGAGG
    GATGGCGGAT CTGGGAACAG TACAATTATA GTGAGCAGAA GTGGAGAGGA TGAATCACAA
    GAGGACGTGC TGATGGATGA AGCTCCTTCC AACCTCAGCC AAGCTTCCAC CTTGCAGGCC
    AACCGAGAAG ATTCCATGAA TATCCTGGAC CCTGAGGATG AGGAGGAGCA CACTCAGGAA
    GAGGACAGCA GTGGCAGTAA CGAGGATGAG GATGATAGTC AGGATGAAGA GGAGGAGGAG
    GAGGAAGATG AGGAAGATGA TCAGGAGGAT GATGAAGGTG AAGAGGGAGA TGAAGACGAT
    GACGACGATG GCTCTGAAAT GGAATTGGAT GAGGATTACC CTGATATGAA CGCTTCTCCC
    TTGGTCCGAT TTGAGCGCTT TGACCGGGAG GATGATCTCA TCATTGAGTT TGACAACATG
    TTCTCCAGTG CTACAGACAT CCCCCCATCC CCAGGAAATA TCCCTACCAC CCACCCACTG
    ATGGTACGCC ATGCAGATCA CAGTTCCCTG ACACTGGGCA GTGGCTCCTC TACAACACGT
    CTCACCCAGG GCATTGGGCG CAGTCAAAGG ACCCTGAGAC AGCTGACAGC CAACACAGGC
    CATACCATTC ACGTTCACTA CCCTGGGAAT CGCCAGCCCA ATCCTCCTCT TATACTCCAG
    AGGTTGCTTG GTCCCTCTGC TGCTGCTGAC ATCCTTCAGC TGAGCAGCAG CCTTCCCCTA
    CAAAGCCGAG GCCGAGCCCG CCTCCTAGTG GGCAACGATG ACGTCCACAT CATCGCCCGG
    TCTGATGATG AACTACTGGA TGACTTTTTC CATGATCAGA GCACAGCTAC CAGCCAAGCA
    GGGACCCTAT CCAGTATCCC TACAGCCCTG ACTCGCTGGA CAGAGGAATG CAAAGTTCTT
    GATGCTGAGA GCATGCATGA CTGTGTTTCA GTTGTTAAAG TGCCCATTGT CAATCATCTG
    GAATTCCTGA GGGATGAGGA GCTGGAAGAA AGGAGGGAGA AAAGGAGGAA ACAACTAGCT
    GAGGAAGAAA CAAAGATAAC CGACAAAGGC AAAGAAGATA AGGAGAACAG GGATCAGAGT
    GCACAGTGTG CTGCATCTAA GACAAATGAC TCCACTGAAC AGAACCTCTC AGATGGGACT
    CCTATGCCTG ACAGCTACCC AGCAACCCCA TCTTCAACTG ATGTAGCTAC ATCTGAGCCC
    AGGGAGACCC TTGTCACTCT GCAGCCCTCC CAACAGCAAC CAACACTCCC AACCCCACCA
    GCCTTGGGAG AGATTCCTCA AGAGCTGCAG TCCCCAATTG GAGAAGGTGG GAGCTCTACA
    CAGCTATTGA TGCCTGTAGA GCCAGAGGAA TTGGGTCCCA CAAGGCCAAG TGGAGAAGCA
    GAAACAACTC AGATGGAGCT ATCTCCAGCT CCCACTATAA CCTCTCTTTC CCCAGAGAGA
    GCTGAAGATT CTGATGCTCT GACAGCTGTC AGCAGTCAAC TGGAAGGCTC TCCCATGGAT
    ACTAGCAGCC TGGCTTCCTG TACCCTGGAG GAGGCTGTGG GTGACGCCCC AGCAACTGGC
    AGTTCTGAGC AGCCCACAGC AGGCAGCTCC ACTCCTGGGG ATGCCCCACC AGTTGTGACA
    GAAGTACAAG GCAGGGGTGA TGGGTCAGGG GAACCTACCC AGCCCCCTGA GGACAGCTCT
    CCCCCTGCAT CCTCTGAGAG TTCTTCCACC AGAGATTCCG CGGTGGCCAT TTCTGGAGCA
    GATTCCCGGG GAATCCTGGA AGAGCCATTG CCTTCAACAA GCAGTGAAGA AGAAGATCCT
    CTTGCAGGTA TTAGTCTCCC CGAAGGTGTG GACCCCTCTT TTCTGGCCGC TCTGCCTGAT
    GACATTCGCC GAGAAGTCCT ACAGAACCAG CTGGGCATTC GTCCACCAAC ACGAACTGCC
    CCTTCAACAA ATAACTCAGC TCCTGCAGTG GTGGGGAATC CTGGTGTCAC CGAAGTGAGC
    CCTGAGTTCC TGGCTGCTCT GCCTCCAGCC ATCCAGGAGG AAGTACTGGC ACAGCAGAGA
    GCTGAGCAAC AGCGACGGGA ACTAGCACAA AATGCCAGCT CAGACACCCC CATGGACCCT
    GTGACCTTCA TCCAGACTCT GCCCTCAGAC CTGCGCCGTA GTGTCCTAGA GGACATGGAG
    GACAGTGTGT TGGCCGTGAT GCCACCTGAC ATCGCAGCTG AAGCTCAAGC CCTGAGGCGA
    GAGCAAGAAG CCCGGCAGAG GCAGCTCATG CATGAGCGAC TTTTTGGGCA CAGCAGCACC
    TCTGCACTCT CTGCCATTCT CCGAAGCCCA GCCTTCACCA GTCGCCTGAG TGGCAACCGT
    GGGGTCCAAT ACACTCGCCT TGCTGTGCAA AGAGGTGGCA CCTTCCAGAT GGGGGGTAGC
    AGCAGCCATA ATAGGCCTTC TGGCAGTAAT GTGGACACCC TCCTACGTCT TCGAGGACGG
    CTCCTCCTGG ATCACGAAGC CCTGTCTTGT CTCCTGGTCC TACTTTTTGT GGATGAGCCA
    AAACTCAATA CTAGCCGTCT TCACCGAGTA CTGAGAAATC TCTGCTACCA TGCCCAGACC
    CGCCACTGGG TTATCCGTAG CCTGCTTTCC ATCTTGCAGC GAAGCAGTGA GAGTGAGCTT
    TGCATTGAAA CACCCAAGCT CTCCTCAAGT GAGGAAAAGG GCAAAAAGTC AAGCAAGAGC
    TGTGGGTCAA GCAGCCATGA GAACCGGCCC CTGGACCTGC TACACAAGAT GGAGTCTAAG
    AGTTCCAACC AGCTTTCCTG GCTCTCAGTA TCCATGGATG CAGCCCTAGG CTGCAGGACT
    AATATATTCC AGATCCAGCG TTCAGGGGGT CGCAAACATA CTGAGAAACA TGCGAGTAGT
    GGCTCCACTG TCCATATCCA CCCCCAGGCT GCTCCTGTTG TCTGCAGACA TGTTCTGGAT
    ACACTCATTC AATTGGCCAA GGTGTTTCCC AGTCACTTCA CACAGCAGCG GACCAAAGAA
    ACAAACTGTG AGAGTGATCG GGAGAGGGGC AGTAAGGCCT GCAGCCCATG CTCTTCACAG
    TCCACAAGCA GTGGCATTTG CACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG
    AATGTCAGCC GGAAAGGCAA GAACTCCGTG AAGTCAGTGC CAGTGAGCGC TGGTGGTGAG
    GGGGAAACCT CCCCATACAG CCTCGAAGCT TCTCCACTGG GGCAACTCAT GAACATGTTG
    TCACACCCAG TCATCCGCCG GAGCTCTCTC TTAACTGAGA AGCTCCTCAG ACTTCTTTCT
    CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTGTCGGAAG CACAGGCTAA TTCTGGCAGC
    AGTGCTTCCT CCACCACTGT TGCCACTTCA ACCACATCTA CCACCACTAT CAGTGCCACC
    TCCAGCACAT CTACTCTGCC TGCTGCAACC ACCCCTGTCA CTTCTGTTCC AGCCCTGGTT
    GCTGCCACGG CTATATCCAC CATTGCCGTA GCCGCTTCAA CCACAGTGAC TACCCCCACA
    ACTGCTACCA CTACTGTTTC AACTGCTACT ACTACTAAGG CCAGCAAATC TCCAGCTAAA
    GTTGGTGATA TCGGCAGCAG CAGCAGCACA GACTTTAAGA TGGTATCCTC TGGCCTCACT
    GAAAATCAGC TCCAGCTCTC TGTGGAAGTG TTGACATCCC ATTCTTGTTC TGAAGAAGGC
    CTAGAAGATG CAGCAAATGT GTTACTGCAG CTCTCTCGGG GGGACCCTGG GACCCGGGAC
    ACTGTTTTGA AGTTGCTACT GAATGGAGCC CGCCATCTGG GTTATACGCT TTGTAAACAG
    ATAGGAACTC TTCTGGCTGA GCTACGGGAA TACAATCTGG AACAGCAGCG GCGAGCCCAG
    TGTGAAACCC TCTCCCCTGA TGGCATGCCT GAGGAACAGC CACAGACCAC CAAGCTGAAA
    GGCAAAATGC AGAGCAGGTT TGACGTGGCT GAGAATGTGG TAATCGTGGC ATCTCAGAAG
    CGGCCTCTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA
    TCTACCCAGA AGTTCTTCTT GAGGGTACTG CAGGTCATCA TCCAGCTCCG GGATGACACG
    CGCCGTGCTA ACAAGAAGGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA
    GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA
    ATGGTACGTG AGGGTCAGAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCAAGC
    CAGTCAGAGG CATCTGTCCG AAGGGAGGAG TCACCCATGG ATGTGGACCA GCCATCTCCC
    AGTGCTCAAG ATACTCAGTC CATTGGCTCA GATGGAACTC CACAGGGAGA GAAGGAGAAG
    GAGGAGAGGC CACCTGAGTT ACCCCTGCTC AGTGAGCAGC TGAACTTGGA TGAGCTGTGG
    GACATGCTGG GAGAGTGTCT AAAGGAACTG GAGGAATCCC ATGACCAGCA TGCGGTGCTA
    GTGCTACAAC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CAACAGAGCG GGAGAGCAAG
    CCTCCTGTCC GGGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCACCTCCA
    CTCTCCCCTG CCCCTTTAAC CCCAGCCACT CCTTCCTCCC TTGACCCGTT CTTCTCCCGG
    GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTTCTTCGT
    TTTGCAGAGA CTCATCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC CACCCACCTC
    GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT CGATGTCAAG
    CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTGGACGAGG GACTCCGGAA AGAAGACATG
    GCTGTGCACG TTCGTCGTGA TCATGTCTTT GAAGACTCCT ATCGTGAGCT GCACCGGAAA
    TCCCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT
    GCTGGAGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG
    TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGGGTCACAT ACACCATCAA TCCATCTTCT
    CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTTG GACGCATCGT GGCCAAAGCT
    GTGTATGACA ACCGCCTCCT GGAGTGCTAC TTTACTAGGT CCTTCTACAA ACACATTTTG
    GGCAAGTCTG TCAGATATAC AGATATGGAG AGTGAAGACT ACCACTTTTA CCAGGGCCTG
    GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ATCTCACTTT CAGCACTGAG
    GTCCAAGAGT TTGGAGTCTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG
    GTAACAGAGG AAAATAAGAA GGAATATGTA CACCTGGTGT GCCAGATGAG AATGACAGGA
    GCCATCCGCA AACAGCTGGC GGCTTTCTTA GAAGGTTTCT ATGAGATCAT TCCAAAGCGC
    CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTCA TATCAGGACT GCCCACAATC
    GACATTGACG ATTTGAAATC TAACACTGAA TATCACAAGT ACCAGTCCAA CTCTATTCAG
    ATCCAGTGGT TCTGGAGAGC ACTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC
    CAGTTTGTCA CAGGTACTTC CAAGGTGCCC CTGCAAGGTT TTGCTGCCCT TGAAGGCATG
    AATGGCATTC AGAAGTTTCA AATCCATCGA GATGACAGGT CCACAGACCG CCTGCCTTCA
    GCTCACACAT GTTTTAATCA GCTGGACCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC
    CACATGCTAC TATTGGCCAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35633
    Clone ID Related Accession (Same CDS sequence) XM_024987894.1 , XM_010821997.2 , XM_005228212.2 , XM_005228212.3 , XM_024987895.1
    Accession Version XM_005228212.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228212.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATAA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTCCACTT AGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGATTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT
    GTACTTGTGC GAAACTGTAT CCAGGCCATG ATTGACCCTT CCATGGATCC ATACCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CAAGCTACGA TGCTGGTGGC
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG CCGAAAAGAA TGCCCTTTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTGCACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TACAGATGTG
    ACTATGGAAA GTAGTCCCAG CTCATCCATC TCTATGGAGC ACCGGCTGGA TGTTGAATTA
    AGGGCATCAA GTTCCAGCAG CAGTAGCATC TCCTCTGGCC CTGGCCCTCG TCCTGGAGTC
    CAGTGTATTC CACAACGAGC CGCACTTCTC AAATCCATGT TGAATTTCCT CAAGAAGGCC
    ATTCAAGACC CTGCTTTCTC AGATGGCATA CGACATGTGA TGGATGGTTC TCTGCCTACC
    TCCCTGAAAC ACATCATCAG CAATGCAGAA TACTATGGCC CATCACTCTT TCTCCTAGCT
    ACTGAAGTGG TGACTGTGTT TGTATTTCAA GAACCATCAT TGCTTTCCTC ACTCCAGGAC
    AATGGATTGA CAGATGTCAT GCTACATGCA CTGCTTATCA AAGACGTTCC TGCTACCCGT
    GAAGTTCTTG GCTCCCTCCC AAATGTTTTC AGTGCACTCT GCTTGAATGC CCGAGGTCTG
    CAGTCATTTG TACAGTGTCA GCCTTTTGAA CGTCTCTTCA AAGTTCTCCT GTCTCCAGAT
    TACCTCCCAG CCATGCGGAG AAGGAGGAGT TCTGATCCTC TAGGGGATAC TGCCTCCAAC
    CTAGGAAGTG CTGTAGATGA GCTCATGAGA CATCAGCCTA CCCTCAAAAC AGATGCAACG
    ACTGCGATCA TCAAGTTACT TGAAGAAATC TGTAATCTTG GAAGAGACCC TAAATACATC
    TGTCAGAAGC CATCAATCCA GAAGGCAGAT GGCACTGCCG CTGCTCCTCC CCCAAGATCT
    AACCATGCTG CAGAAGAGGC CTCTAGTGAG GATGAGGAGG AAGAGGAAGT ACAGGCCATG
    CAGAGCTTTA ATTCTACCCA GCAGAATGAA ACTGAGCCTA ATCAGCAGGT TGTTGGTACA
    GAGGAGCGTA TTCCTATTCC CCTCATGGAT TACATCCTTA ATGTGATGAA ATTTGTGGAA
    TCTATTCTGA GCAACAATAC AACTGATGAC CACTGCCAGG AATTTGTGAA TCAGAAAGGA
    CTCTTGCCTT TGGTCACCAT TTTGGGTCTT CCCAATCTGC CCATTGACTT TCCCACGTCT
    GCAGCCTGTC AGGCTGTTGC AGGTGTCTGC AAATCCATAT TGACACTGTC ACATGAACCC
    AAAGTCCTCC AGGAAGGTCT CCTTCAGTTG GACTCCATTC TTTCCTCCTT GGAGCCCTTA
    CACCGCCCAA TAGAATCCCC CGGGGGCTCA GTGTTGTTGC GAGAACTGGC TTGTGCTGGC
    AATGTTGCTG ATGCTACCCT CTCAGCTCAA GCCACACCTC TACTACATGC ACTCACTGCT
    GCCCATGCTT ACATCATGAT GTTTGTTCAT ACTTGCAGGG TTGGACAGAG TGAAATTCGT
    TCCATCTCCA TAAACCAGTG GGGCTCTCAG TTGGGTCTGA GTGTTCTGAG CAAGCTGAGC
    CAGTTATACT GTTCCCTGGT ATGGGAAAGC ACTGTCCTCC TCTCCCTCTG TACCCCAAAC
    AGCTTGCCAT CTGGGTGTGA ATTTGGCCAG GCAGACATGC AGAAACTGGT TCCAAAGGAT
    GAGAAGACAG GTACGACCCA GGGCGGAAAA AGATCAGATG GGGATCAGGA TGGAACCACT
    GGAAGTATGG ATGCTTCTAC CCAGGGCTTG TTGGAAGGCA TTGGGCTTGA TGGTGACACA
    CTGGCTCCTA TGGAGACAGA CGAACCTACT GCTTCAGACT CTAAGGGCAA ATCTAAAATC
    ACACCTGCCA TGGCTGCCAG AATCAAGCAA ATCAAGCCAT TGTTGTCAGC TTCCTCTAGA
    TTAGGCAGAG CACTGGCTGA GCTGTTTGGA CTCCTTGTTA AACTTTGTGT GGGGTCTCCT
    GTCCGCCAGA GAAGAAGCCA TCATGCTGCC AGCACCACCA CAGCACCAAC ACCTGCTGCT
    CGATCAACGG CCTCAGCCCT CACTAAGCTC CTCACAAAAG GCTTATCATG GCAGCCTCCA
    CCATATACGC CTACTCCACG CTTCAGGCTG ACCTTCTTCA TCTGTTCAGT TGGTTTTACA
    TCCCCAATGC TGTTTGATGA GAGGAAGTAT CCCTACCACC TCATGCTGCA GAAATTTCTC
    TGCTCTGGAG GCCACAATGC CCTTTTTGAA ACATTCAACT GGGCTCTGTC TATGGGAGGA
    AAAGTTCCTG TTGCTGAGGG ATTAGAACAT TCAGACTTAC CTGATGGCAC AGGAGAATTC
    CTAGATGCCT GGCTAATGCT GGTGGAGAAG ATGGTAAATC CCACCACTGT GCTTGAATCT
    CCACATTCAC TGCCTGCCAA ATTGCCTGGA GGTGTTCAGA ACTTTCCACA GTTCAGTGCC
    CTCCGCTTCC TTGTGGTAAC TCAGAAGGCA GCATTTACTT GCATCAAGAA CCTGTGGAAC
    CGGAAACCCT TGAAAGTGTA TGGTGGGCGA ATGGCTGAGT CCATGCTAGC CATCCTGTGC
    CACATCCTCC GAGGAGAACC TGTGATTCGA GAGAGACTGA GCAAAGAAAA GGAGGGATCT
    CGAGGAGAAG AGGATACAGG ACAAGAAGAA GGTGGCTCTC GCCGGGAACC TCAAGTCAAC
    CAGCAACAAC TACAACAGCT CATGGACATG GGCTTCACAA GGGAACATGC CATGGAGGCC
    CTGTTGAACA CCAGTACCAT GGAGCAGGCC ACAGAGTACC TTCTAACCCA CCCTCCCCCG
    ATCATGGGAG GAGTTGTTCG GGATCTGAGT ATGTCTGAAG AGGACCAGAT GATGAGAGCG
    ATTGCCATGT CTCTGGGACA GGATATCCCA ATGGATCAAA GAGCAGAGTC ACCTGAGGAA
    GTTGCTTGCC GAAAGGAAGA GGAGGAACGG AAAGCTCGAG AAAAGCAGGA AGAGGAAGAG
    GCTAAATGTC TAGAGAAGTT CCAGGATGCT GATCCATTGG AGCAAGATGA GCTCCACACT
    TTCACAGATA CAATGTTGCC AGGCTGCTTT CACCTTCTTG ATGAGTTGCC AGACACTGTA
    TACCGAGTGT GTGATCTGAT CATGACAGCA ATCAAACGAA ATGGAGCAGA CTATCGAGAC
    ATGATTCTGA AGCAAGTAGT TAATCAGGTG TGGGAAGCTG CTGATGTATT GATCAAAGCT
    GCTCTTCCCC TGACAACAAG TGACACAAAA ACTGTATCAG AGTGGATCAG TCAGATGGCC
    ACTCTACCCC AGGCCTCTAA TTTGGCCACT AGAATCTTGC TTTTAACACT GCTTTTTGAG
    GAATTGAAGC TACCTTGTGC TTGGGTGGTT GAATCTAGTG GCATCCTTAA TGTCCTAATC
    AAACTCTTGG AAGTGGTTCA GCCCTGCCTA CAGGCCGCCA AGGAACAAAA GGAGGTCCAG
    ACCCCAAAGT GGATCACTCC AGTGTTGCTC CTGATTGATT TCTATGAAAA AACAGCCATT
    TCCTCAAAGA GGAGAGCCCA AATGACTAAG TATCTACAGT CTAACAACAA CAACTGGCGC
    TGGTTTGATG ACCGCTCTGG ACGTTGGTGT AGTTACAGTG CAAGCAACAA CAGCACTATT
    GATTCTGCCT GGAAATCTGG AGAGACAAGT GTGCGTTTCA CTGCAGGCCG AAGAAGATAC
    ACTGTCCAGT TCACTACAAT GGTACAGGTT AATGAGGAAA CAGGGAACCG GCGTCCTGTG
    ATGCTGACAC TCCTGAGGGT ACCCCGATTG AATAAAAATT CTAAAAACAG CAATGGACAG
    GAACTAGAGA AGACACTGGA AGAAAGCAAA GAAATGGATA TCAAACGTAA AGAAAATAAA
    ACCAGTGATA CCCCCTTGGC CCTAGACAGT ACAAATACTG AAAAGGAGAC AAGCCTGGAA
    GAAACAAAAA TTGGGGAGAT CTTGATCCAG GGCCTGACAG AGGATATGGT GACTGTTTTA
    ATCCGGGCCT GTGTGAGCAT GCTAGGCGTC CCTGTGGATC CAGACACTTT GCATGCCACC
    CTTCGCCTAT GCCTGAGGCT CACCCGAGAC CACAAATATG CCATGATGTT TGCAGAGCTG
    AAGAGTACTC GCATGATCTT AAATTTGACC CAGAGTTCAG GCTTCAATGG GTTTACTCCC
    CTGGTTACCC TTCTCTTAAG ACACATCATT GAGGACCCCT GCACCCTGCG CCATACCATG
    GAAAAGGTTG TTCGCTCAGC AGCTACAAGT GGAGCTGGTA GCACTACTTC TGGTGTTGTA
    TCTGGCAGCC TTGGCTCTCG GGAGATCAAC TACATCCTTC GTGTCCTTGG ACCAGCCGCA
    TGCCGCAACC CAGACATTTT CACAGAAGTG GCCAACTGCT GTATCCGCAT TGCCCTTCCA
    GCCCCTCGCG GCTCAGGAAC TGCTTCAGAT GATGAGTTTG AGAATCTCAG AATTAAAGGC
    CCGAATGCTG TACAGCTGGT GAAAACAACC CCTTTGAAGC CCTCACCTCT GCCTGTCATT
    CCTGATACTA TCAAGGAAGT GATCTATGAC ATGCTAAATG CCCTGGCTGC ATACCATGCT
    CCAGAGGAAG CAGACAAATC TGATCCTAAA CCAGGGGGTA TGACTCAAGA GGTTGGCCAG
    CTCCTGCAAG ACATGGGTGA TGACGTATAC CAGCAGTACC GGTCACTTAC CCGTCAGAGC
    AGTGACTTTG ATGCACAGAC AGGATTTTCC ATTAATAGTC AGGTCTTTGC TGCAGATGGT
    TCCTCCACTG AGACTTCCGC ATCTGGGGTA TCCCAAGGAG AAGTGTCAAC TCCGGAGGAG
    TCTCGGGATG GGAAGAAAGA TAAAGAAGGG GACCGGGCCT CTGAGGAAGG CAAGCAGAAA
    GGCAAGGGCA GCAAACCTTT AATGCCCACC TCCACTATTC TTCGGCTTCT GGCAGAGTTG
    GTGAGATCCT ATGTTGGTAT TGCTACCCTG ATTGCCAACT ACAGCTACAC TGTGGGCCAG
    TCTGAGCTGA TCAAAGAGGA CTGCAGTGTG CTAGCTTTTG TCTTGGACCA CCTCCTCCCG
    CATACCCAGA ATGCAGAAGA CAAAGACACA CCGGCGCTGG CCCGCCTGTT CCTCGCAAGC
    CTGGCTGCCT CAGGGAGTGG CACAGATGCC CAGGTGGCCC TTGTGAATGA AGTAAAAGCA
    GCCCTGGGAA GGGCACTGGC TATGGCTGAG AGTACAGAGA AACATGCCAG ACTTCAGGCA
    GTGATGTGTA TCATCAGTAC TATCATGGAG TCCTGCCCCT CCACCTCCAG TTTCTACAGC
    AGTGCAACAG CCAAGACCCA ACACAACGGC ATGAACAACA TCATTCGACT CTTCCTGAAG
    AAGGGACTGG TTAATGACCT GGCCAGAGTG CCTCACAGCC TGGACCTGTC CAGTCCCAAC
    ATGGCCAACA CTGTCAATGC TGCTCTGAAG CCTTTGGAAA CACTTTCCCG GATTGTGAAC
    CAGCCCAGTA GCCTTTTTGG CAGTAAGAGT GCTTCTAGCA AGAGCAAGTC TGAGCAGGAT
    GCCCAAGGAG CTGCTCAGGA CTCTAATAGC AACCAACAGG ACCCAGGCGA GCCTGGGGAA
    ACAGAAGTGC AGGAGGAGGA TCATGATGTC ACTCAGACAG AGGTGGCAGA TGGGGATATC
    ATGGATGGGG AGGCTGAAAC CGACTCAGTG GTGATTGCTG GGCAGCCTGA GGTGCTCAGT
    TCACAAGAGA TGCAGGTTGA GAATGAGCTG GAGGACCTGA TAGATGAGTT GCTTGAGAGG
    GATGGCGGAT CTGGGAACAG TACAATTATA GTGAGCAGAA GTGGAGAGGA TGAATCACAA
    GAGGACGTGC TGATGGATGA AGCTCCTTCC AACCTCAGCC AAGCTTCCAC CTTGCAGGCC
    AACCGAGAAG ATTCCATGAA TATCCTGGAC CCTGAGGATG AGGAGGAGCA CACTCAGGAA
    GAGGACAGCA GTGGCAGTAA CGAGGATGAG GATGATAGTC AGGATGAAGA GGAGGAGGAG
    GAGGAAGATG AGGAAGATGA TCAGGAGGAT GATGAAGGTG AAGAGGGAGA TGAAGACGAT
    GACGACGATG GCTCTGAAAT GGAATTGGAT GAGGATTACC CTGATATGAA CGCTTCTCCC
    TTGGTCCGAT TTGAGCGCTT TGACCGGGAG GATGATCTCA TCATTGAGTT TGACAACATG
    TTCTCCAGTG CTACAGACAT CCCCCCATCC CCAGGAAATA TCCCTACCAC CCACCCACTG
    ATGGTACGCC ATGCAGATCA CAGTTCCCTG ACACTGGGCA GTGGCTCCTC TACAACACGT
    CTCACCCAGG GCATTGGGCG CAGTCAAAGG ACCCTGAGAC AGCTGACAGC CAACACAGGC
    CATACCATTC ACGTTCACTA CCCTGGGAAT CGCCAGCCCA ATCCTCCTCT TATACTCCAG
    AGGTTGCTTG GTCCCTCTGC TGCTGCTGAC ATCCTTCAGC TGAGCAGCAG CCTTCCCCTA
    CAAAGCCGAG GCCGAGCCCG CCTCCTAGTG GGCAACGATG ACGTCCACAT CATCGCCCGG
    TCTGATGATG AACTACTGGA TGACTTTTTC CATGATCAGA GCACAGCTAC CAGCCAAGCA
    GGGACCCTAT CCAGTATCCC TACAGCCCTG ACTCGCTGGA CAGAGGAATG CAAAGTTCTT
    GATGCTGAGA GCATGCATGA CTGTGTTTCA GTTGTTAAAG TGCCCATTGT CAATCATCTG
    GAATTCCTGA GGGATGAGGA GCTGGAAGAA AGGAGGGAGA AAAGGAGGAA ACAACTAGCT
    GAGGAAGAAA CAAAGATAAC CGACAAAGGC AAAGAAGATA AGGAGAACAG GGATCAGAGT
    GCACAGTGTG CTGCATCTAA GACAAATGAC TCCACTGAAC AGAACCTCTC AGATGGGACT
    CCTATGCCTG ACAGCTACCC AGCAACCCCA TCTTCAACTG ATGTAGCTAC ATCTGAGCCC
    AGGGAGACCC TTGTCACTCT GCAGCCCTCC CAACAGCAAC CAACACTCCC AACCCCACCA
    GCCTTGGGAG AGATTCCTCA AGAGCTGCAG TCCCCAATTG GAGAAGGTGG GAGCTCTACA
    CAGCTATTGA TGCCTGTAGA GCCAGAGGAA TTGGGTCCCA CAAGGCCAAG TGGAGAAGCA
    GAAACAACTC AGATGGAGCT ATCTCCAGCT CCCACTATAA CCTCTCTTTC CCCAGAGAGA
    GCTGAAGATT CTGATGCTCT GACAGCTGTC AGCAGTCAAC TGGAAGGCTC TCCCATGGAT
    ACTAGCAGCC TGGCTTCCTG TACCCTGGAG GAGGCTGTGG GTGACGCCCC AGCAACTGGC
    AGTTCTGAGC AGCCCACAGC AGGCAGCTCC ACTCCTGGGG ATGCCCCACC AGTTGTGACA
    GAAGTACAAG GCAGGGGTGA TGGGTCAGGG GAACCTACCC AGCCCCCTGA GGACAGCTCT
    CCCCCTGCAT CCTCTGAGAG TTCTTCCACC AGAGATTCCG CGGTGGCCAT TTCTGGAGCA
    GATTCCCGGG GAATCCTGGA AGAGCCATTG CCTTCAACAA GCAGTGAAGA AGAAGATCCT
    CTTGCAGGTA TTAGTCTCCC CGAAGGTGTG GACCCCTCTT TTCTGGCCGC TCTGCCTGAT
    GACATTCGCC GAGAAGTCCT ACAGAACCAG CTGGGCATTC GTCCACCAAC ACGAACTGCC
    CCTTCAACAA ATAACTCAGC TCCTGCAGTG GTGGGGAATC CTGGTGTCAC CGAAGTGAGC
    CCTGAGTTCC TGGCTGCTCT GCCTCCAGCC ATCCAGGAGG AAGTACTGGC ACAGCAGAGA
    GCTGAGCAAC AGCGACGGGA ACTAGCACAA AATGCCAGCT CAGACACCCC CATGGACCCT
    GTGACCTTCA TCCAGACTCT GCCCTCAGAC CTGCGCCGTA GTGTCCTAGA GGACATGGAG
    GACAGTGTGT TGGCCGTGAT GCCACCTGAC ATCGCAGCTG AAGCTCAAGC CCTGAGGCGA
    GAGCAAGAAG CCCGGCAGAG GCAGCTCATG CATGAGCGAC TTTTTGGGCA CAGCAGCACC
    TCTGCACTCT CTGCCATTCT CCGAAGCCCA GCCTTCACCA GTCGCCTGAG TGGCAACCGT
    GGGGTCCAAT ACACTCGCCT TGCTGTGCAA AGAGGTGGCA CCTTCCAGAT GGGGGGTAGC
    AGCAGCCATA ATAGGCCTTC TGGCAGTAAT GTGGACACCC TCCTACGTCT TCGAGGACGG
    CTCCTCCTGG ATCACGAAGC CCTGTCTTGT CTCCTGGTCC TACTTTTTGT GGATGAGCCA
    AAACTCAATA CTAGCCGTCT TCACCGAGTA CTGAGAAATC TCTGCTACCA TGCCCAGACC
    CGCCACTGGG TTATCCGTAG CCTGCTTTCC ATCTTGCAGC GAAGCAGTGA GAGTGAGCTT
    TGCATTGAAA CACCCAAGCT CTCCTCAAGT GAGGAAAAGG GCAAAAAGTC AAGCAAGAGC
    TGTGGGTCAA GCAGCCATGA GAACCGGCCC CTGGACCTGC TACACAAGAT GGAGTCTAAG
    AGTTCCAACC AGCTTTCCTG GCTCTCAGTA TCCATGGATG CAGCCCTAGG CTGCAGGACT
    AATATATTCC AGATCCAGCG TTCAGGGGGT CGCAAACATA CTGAGAAACA TGCGAGTAGT
    GGCTCCACTG TCCATATCCA CCCCCAGGCT GCTCCTGTTG TCTGCAGACA TGTTCTGGAT
    ACACTCATTC AATTGGCCAA GGTGTTTCCC AGTCACTTCA CACAGCAGCG GACCAAAGAA
    ACAAACTGTG AGAGTGATCG GGAGAGGGGC AGTAAGGCCT GCAGCCCATG CTCTTCACAG
    TCCACAAGCA GTGGCATTTG CACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG
    AATGTCAGCC GGAAAGGCAA GAACTCCGTG AAGTCAGTGC CAGTGAGCGC TGGTGGTGAG
    GGGGAAACCT CCCCATACAG CCTCGAAGCT TCTCCACTGG GGCAACTCAT GAACATGTTG
    TCACACCCAG TCATCCGCCG GAGCTCTCTC TTAACTGAGA AGCTCCTCAG ACTTCTTTCT
    CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTGTCGGAAG CACAGGCTAA TTCTGGCAGC
    AGTGCTTCCT CCACCACTGT TGCCACTTCA ACCACATCTA CCACCACTAT CAGTGCCACC
    TCCAGCACAT CTACTCTGCC TGCTGCAACC ACCCCTGTCA CTTCTGTTCC AGCCCTGGTT
    GCTGCCACGG CTATATCCAC CATTGCCGTA GCCGCTTCAA CCACAGTGAC TACCCCCACA
    ACTGCTACCA CTACTGTTTC AACTGCTACT ACTACTAAGG CCAGCAAATC TCCAGCTAAA
    GTTGGTGATA TCGGCAGCAG CAGCAGCACA GACTTTAAGA TGGTATCCTC TGGCCTCACT
    GAAAATCAGC TCCAGCTCTC TGTGGAAGTG TTGACATCCC ATTCTTGTTC TGAAGAAGGC
    CTAGAAGATG CAGCAAATGT GTTACTGCAG CTCTCTCGGG GGGACCCTGG GACCCGGGAC
    ACTGTTTTGA AGTTGCTACT GAATGGAGCC CGCCATCTGG GTTATACGCT TTGTAAACAG
    ATAGGAACTC TTCTGGCTGA GCTACGGGAA TACAATCTGG AACAGCAGCG GCGAGCCCAG
    TGTGAAACCC TCTCCCCTGA TGGCATGCCT GAGGAACAGC CACAGACCAC CAAGCTGAAA
    GGCAAAATGC AGAGCAGGTT TGACGTGGCT GAGAATGTGG TAATCGTGGC ATCTCAGAAG
    CGGCCTCTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA
    TCTACCCAGA AGTTCTTCTT GAGGGTACTG CAGGTCATCA TCCAGCTCCG GGATGACACG
    CGCCGTGCTA ACAAGAAGGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA
    GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA
    ATGGTACGTG AGGGTCAGAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCAAGC
    CAGTCAGAGG CATCTGTCCG AAGGGAGGAG TCACCCATGG ATGTGGACCA GCCATCTCCC
    AGTGCTCAAG ATACTCAGTC CATTGGCTCA GATGGAACTC CACAGGGAGA GAAGGAGAAG
    GAGGAGAGGC CACCTGAGTT ACCCCTGCTC AGTGAGCAGC TGAACTTGGA TGAGCTGTGG
    GACATGCTGG GAGAGTGTCT AAAGGAACTG GAGGAATCCC ATGACCAGCA TGCGGTGCTA
    GTGCTACAAC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CAACAGAGCG GGAGAGCAAG
    CCTCCTGTCC GGGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCACCTCCA
    CTCTCCCCTG CCCCTTTAAC CCCAGCCACT CCTTCCTCCC TTGACCCGTT CTTCTCCCGG
    GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTTCTTCGT
    TTTGCAGAGA CTCATCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC CACCCACCTC
    GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT CGATGTCAAG
    CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTGGACGAGG GACTCCGGAA AGAAGACATG
    GCTGTGCACG TTCGTCGTGA TCATGTCTTT GAAGACTCCT ATCGTGAGCT GCACCGGAAA
    TCCCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT
    GCTGGAGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG
    TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGGGTCACAT ACACCATCAA TCCATCTTCT
    CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTTG GACGCATCGT GGCCAAAGCT
    GTGTATGACA ACCGCCTCCT GGAGTGCTAC TTTACTAGGT CCTTCTACAA ACACATTTTG
    GGCAAGTCTG TCAGATATAC AGATATGGAG AGTGAAGACT ACCACTTTTA CCAGGGCCTG
    GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ATCTCACTTT CAGCACTGAG
    GTCCAAGAGT TTGGAGTCTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG
    GTAACAGAGG AAAATAAGAA GGAATATGTA CACCTGGTGT GCCAGATGAG AATGACAGGA
    GCCATCCGCA AACAGCTGGC GGCTTTCTTA GAAGGTTTCT ATGAGATCAT TCCAAAGCGC
    CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTCA TATCAGGACT GCCCACAATC
    GACATTGACG ATTTGAAATC TAACACTGAA TATCACAAGT ACCAGTCCAA CTCTATTCAG
    ATCCAGTGGT TCTGGAGAGC ACTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC
    CAGTTTGTCA CAGGTACTTC CAAGGTGCCC CTGCAAGGTT TTGCTGCCCT TGAAGGCATG
    AATGGCATTC AGAAGTTTCA AATCCATCGA GATGACAGGT CCACAGACCG CCTGCCTTCA
    GCTCACACAT GTTTTAATCA GCTGGACCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC
    CACATGCTAC TATTGGCCAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228269.1
    CDS370..13623
    Translation

    Target ORF information:

    RefSeq Version XM_005228212.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228212.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATAA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTCCACTT AGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGATTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT
    GTACTTGTGC GAAACTGTAT CCAGGCCATG ATTGACCCTT CCATGGATCC ATACCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CAAGCTACGA TGCTGGTGGC
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG CCGAAAAGAA TGCCCTTTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTGCACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TACAGATGTG
    ACTATGGAAA GTAGTCCCAG CTCATCCATC TCTATGGAGC ACCGGCTGGA TGTTGAATTA
    AGGGCATCAA GTTCCAGCAG CAGTAGCATC TCCTCTGGCC CTGGCCCTCG TCCTGGAGTC
    CAGTGTATTC CACAACGAGC CGCACTTCTC AAATCCATGT TGAATTTCCT CAAGAAGGCC
    ATTCAAGACC CTGCTTTCTC AGATGGCATA CGACATGTGA TGGATGGTTC TCTGCCTACC
    TCCCTGAAAC ACATCATCAG CAATGCAGAA TACTATGGCC CATCACTCTT TCTCCTAGCT
    ACTGAAGTGG TGACTGTGTT TGTATTTCAA GAACCATCAT TGCTTTCCTC ACTCCAGGAC
    AATGGATTGA CAGATGTCAT GCTACATGCA CTGCTTATCA AAGACGTTCC TGCTACCCGT
    GAAGTTCTTG GCTCCCTCCC AAATGTTTTC AGTGCACTCT GCTTGAATGC CCGAGGTCTG
    CAGTCATTTG TACAGTGTCA GCCTTTTGAA CGTCTCTTCA AAGTTCTCCT GTCTCCAGAT
    TACCTCCCAG CCATGCGGAG AAGGAGGAGT TCTGATCCTC TAGGGGATAC TGCCTCCAAC
    CTAGGAAGTG CTGTAGATGA GCTCATGAGA CATCAGCCTA CCCTCAAAAC AGATGCAACG
    ACTGCGATCA TCAAGTTACT TGAAGAAATC TGTAATCTTG GAAGAGACCC TAAATACATC
    TGTCAGAAGC CATCAATCCA GAAGGCAGAT GGCACTGCCG CTGCTCCTCC CCCAAGATCT
    AACCATGCTG CAGAAGAGGC CTCTAGTGAG GATGAGGAGG AAGAGGAAGT ACAGGCCATG
    CAGAGCTTTA ATTCTACCCA GCAGAATGAA ACTGAGCCTA ATCAGCAGGT TGTTGGTACA
    GAGGAGCGTA TTCCTATTCC CCTCATGGAT TACATCCTTA ATGTGATGAA ATTTGTGGAA
    TCTATTCTGA GCAACAATAC AACTGATGAC CACTGCCAGG AATTTGTGAA TCAGAAAGGA
    CTCTTGCCTT TGGTCACCAT TTTGGGTCTT CCCAATCTGC CCATTGACTT TCCCACGTCT
    GCAGCCTGTC AGGCTGTTGC AGGTGTCTGC AAATCCATAT TGACACTGTC ACATGAACCC
    AAAGTCCTCC AGGAAGGTCT CCTTCAGTTG GACTCCATTC TTTCCTCCTT GGAGCCCTTA
    CACCGCCCAA TAGAATCCCC CGGGGGCTCA GTGTTGTTGC GAGAACTGGC TTGTGCTGGC
    AATGTTGCTG ATGCTACCCT CTCAGCTCAA GCCACACCTC TACTACATGC ACTCACTGCT
    GCCCATGCTT ACATCATGAT GTTTGTTCAT ACTTGCAGGG TTGGACAGAG TGAAATTCGT
    TCCATCTCCA TAAACCAGTG GGGCTCTCAG TTGGGTCTGA GTGTTCTGAG CAAGCTGAGC
    CAGTTATACT GTTCCCTGGT ATGGGAAAGC ACTGTCCTCC TCTCCCTCTG TACCCCAAAC
    AGCTTGCCAT CTGGGTGTGA ATTTGGCCAG GCAGACATGC AGAAACTGGT TCCAAAGGAT
    GAGAAGACAG GTACGACCCA GGGCGGAAAA AGATCAGATG GGGATCAGGA TGGAACCACT
    GGAAGTATGG ATGCTTCTAC CCAGGGCTTG TTGGAAGGCA TTGGGCTTGA TGGTGACACA
    CTGGCTCCTA TGGAGACAGA CGAACCTACT GCTTCAGACT CTAAGGGCAA ATCTAAAATC
    ACACCTGCCA TGGCTGCCAG AATCAAGCAA ATCAAGCCAT TGTTGTCAGC TTCCTCTAGA
    TTAGGCAGAG CACTGGCTGA GCTGTTTGGA CTCCTTGTTA AACTTTGTGT GGGGTCTCCT
    GTCCGCCAGA GAAGAAGCCA TCATGCTGCC AGCACCACCA CAGCACCAAC ACCTGCTGCT
    CGATCAACGG CCTCAGCCCT CACTAAGCTC CTCACAAAAG GCTTATCATG GCAGCCTCCA
    CCATATACGC CTACTCCACG CTTCAGGCTG ACCTTCTTCA TCTGTTCAGT TGGTTTTACA
    TCCCCAATGC TGTTTGATGA GAGGAAGTAT CCCTACCACC TCATGCTGCA GAAATTTCTC
    TGCTCTGGAG GCCACAATGC CCTTTTTGAA ACATTCAACT GGGCTCTGTC TATGGGAGGA
    AAAGTTCCTG TTGCTGAGGG ATTAGAACAT TCAGACTTAC CTGATGGCAC AGGAGAATTC
    CTAGATGCCT GGCTAATGCT GGTGGAGAAG ATGGTAAATC CCACCACTGT GCTTGAATCT
    CCACATTCAC TGCCTGCCAA ATTGCCTGGA GGTGTTCAGA ACTTTCCACA GTTCAGTGCC
    CTCCGCTTCC TTGTGGTAAC TCAGAAGGCA GCATTTACTT GCATCAAGAA CCTGTGGAAC
    CGGAAACCCT TGAAAGTGTA TGGTGGGCGA ATGGCTGAGT CCATGCTAGC CATCCTGTGC
    CACATCCTCC GAGGAGAACC TGTGATTCGA GAGAGACTGA GCAAAGAAAA GGAGGGATCT
    CGAGGAGAAG AGGATACAGG ACAAGAAGAA GGTGGCTCTC GCCGGGAACC TCAAGTCAAC
    CAGCAACAAC TACAACAGCT CATGGACATG GGCTTCACAA GGGAACATGC CATGGAGGCC
    CTGTTGAACA CCAGTACCAT GGAGCAGGCC ACAGAGTACC TTCTAACCCA CCCTCCCCCG
    ATCATGGGAG GAGTTGTTCG GGATCTGAGT ATGTCTGAAG AGGACCAGAT GATGAGAGCG
    ATTGCCATGT CTCTGGGACA GGATATCCCA ATGGATCAAA GAGCAGAGTC ACCTGAGGAA
    GTTGCTTGCC GAAAGGAAGA GGAGGAACGG AAAGCTCGAG AAAAGCAGGA AGAGGAAGAG
    GCTAAATGTC TAGAGAAGTT CCAGGATGCT GATCCATTGG AGCAAGATGA GCTCCACACT
    TTCACAGATA CAATGTTGCC AGGCTGCTTT CACCTTCTTG ATGAGTTGCC AGACACTGTA
    TACCGAGTGT GTGATCTGAT CATGACAGCA ATCAAACGAA ATGGAGCAGA CTATCGAGAC
    ATGATTCTGA AGCAAGTAGT TAATCAGGTG TGGGAAGCTG CTGATGTATT GATCAAAGCT
    GCTCTTCCCC TGACAACAAG TGACACAAAA ACTGTATCAG AGTGGATCAG TCAGATGGCC
    ACTCTACCCC AGGCCTCTAA TTTGGCCACT AGAATCTTGC TTTTAACACT GCTTTTTGAG
    GAATTGAAGC TACCTTGTGC TTGGGTGGTT GAATCTAGTG GCATCCTTAA TGTCCTAATC
    AAACTCTTGG AAGTGGTTCA GCCCTGCCTA CAGGCCGCCA AGGAACAAAA GGAGGTCCAG
    ACCCCAAAGT GGATCACTCC AGTGTTGCTC CTGATTGATT TCTATGAAAA AACAGCCATT
    TCCTCAAAGA GGAGAGCCCA AATGACTAAG TATCTACAGT CTAACAACAA CAACTGGCGC
    TGGTTTGATG ACCGCTCTGG ACGTTGGTGT AGTTACAGTG CAAGCAACAA CAGCACTATT
    GATTCTGCCT GGAAATCTGG AGAGACAAGT GTGCGTTTCA CTGCAGGCCG AAGAAGATAC
    ACTGTCCAGT TCACTACAAT GGTACAGGTT AATGAGGAAA CAGGGAACCG GCGTCCTGTG
    ATGCTGACAC TCCTGAGGGT ACCCCGATTG AATAAAAATT CTAAAAACAG CAATGGACAG
    GAACTAGAGA AGACACTGGA AGAAAGCAAA GAAATGGATA TCAAACGTAA AGAAAATAAA
    ACCAGTGATA CCCCCTTGGC CCTAGACAGT ACAAATACTG AAAAGGAGAC AAGCCTGGAA
    GAAACAAAAA TTGGGGAGAT CTTGATCCAG GGCCTGACAG AGGATATGGT GACTGTTTTA
    ATCCGGGCCT GTGTGAGCAT GCTAGGCGTC CCTGTGGATC CAGACACTTT GCATGCCACC
    CTTCGCCTAT GCCTGAGGCT CACCCGAGAC CACAAATATG CCATGATGTT TGCAGAGCTG
    AAGAGTACTC GCATGATCTT AAATTTGACC CAGAGTTCAG GCTTCAATGG GTTTACTCCC
    CTGGTTACCC TTCTCTTAAG ACACATCATT GAGGACCCCT GCACCCTGCG CCATACCATG
    GAAAAGGTTG TTCGCTCAGC AGCTACAAGT GGAGCTGGTA GCACTACTTC TGGTGTTGTA
    TCTGGCAGCC TTGGCTCTCG GGAGATCAAC TACATCCTTC GTGTCCTTGG ACCAGCCGCA
    TGCCGCAACC CAGACATTTT CACAGAAGTG GCCAACTGCT GTATCCGCAT TGCCCTTCCA
    GCCCCTCGCG GCTCAGGAAC TGCTTCAGAT GATGAGTTTG AGAATCTCAG AATTAAAGGC
    CCGAATGCTG TACAGCTGGT GAAAACAACC CCTTTGAAGC CCTCACCTCT GCCTGTCATT
    CCTGATACTA TCAAGGAAGT GATCTATGAC ATGCTAAATG CCCTGGCTGC ATACCATGCT
    CCAGAGGAAG CAGACAAATC TGATCCTAAA CCAGGGGGTA TGACTCAAGA GGTTGGCCAG
    CTCCTGCAAG ACATGGGTGA TGACGTATAC CAGCAGTACC GGTCACTTAC CCGTCAGAGC
    AGTGACTTTG ATGCACAGAC AGGATTTTCC ATTAATAGTC AGGTCTTTGC TGCAGATGGT
    TCCTCCACTG AGACTTCCGC ATCTGGGGTA TCCCAAGGAG AAGTGTCAAC TCCGGAGGAG
    TCTCGGGATG GGAAGAAAGA TAAAGAAGGG GACCGGGCCT CTGAGGAAGG CAAGCAGAAA
    GGCAAGGGCA GCAAACCTTT AATGCCCACC TCCACTATTC TTCGGCTTCT GGCAGAGTTG
    GTGAGATCCT ATGTTGGTAT TGCTACCCTG ATTGCCAACT ACAGCTACAC TGTGGGCCAG
    TCTGAGCTGA TCAAAGAGGA CTGCAGTGTG CTAGCTTTTG TCTTGGACCA CCTCCTCCCG
    CATACCCAGA ATGCAGAAGA CAAAGACACA CCGGCGCTGG CCCGCCTGTT CCTCGCAAGC
    CTGGCTGCCT CAGGGAGTGG CACAGATGCC CAGGTGGCCC TTGTGAATGA AGTAAAAGCA
    GCCCTGGGAA GGGCACTGGC TATGGCTGAG AGTACAGAGA AACATGCCAG ACTTCAGGCA
    GTGATGTGTA TCATCAGTAC TATCATGGAG TCCTGCCCCT CCACCTCCAG TTTCTACAGC
    AGTGCAACAG CCAAGACCCA ACACAACGGC ATGAACAACA TCATTCGACT CTTCCTGAAG
    AAGGGACTGG TTAATGACCT GGCCAGAGTG CCTCACAGCC TGGACCTGTC CAGTCCCAAC
    ATGGCCAACA CTGTCAATGC TGCTCTGAAG CCTTTGGAAA CACTTTCCCG GATTGTGAAC
    CAGCCCAGTA GCCTTTTTGG CAGTAAGAGT GCTTCTAGCA AGAGCAAGTC TGAGCAGGAT
    GCCCAAGGAG CTGCTCAGGA CTCTAATAGC AACCAACAGG ACCCAGGCGA GCCTGGGGAA
    ACAGAAGTGC AGGAGGAGGA TCATGATGTC ACTCAGACAG AGGTGGCAGA TGGGGATATC
    ATGGATGGGG AGGCTGAAAC CGACTCAGTG GTGATTGCTG GGCAGCCTGA GGTGCTCAGT
    TCACAAGAGA TGCAGGTTGA GAATGAGCTG GAGGACCTGA TAGATGAGTT GCTTGAGAGG
    GATGGCGGAT CTGGGAACAG TACAATTATA GTGAGCAGAA GTGGAGAGGA TGAATCACAA
    GAGGACGTGC TGATGGATGA AGCTCCTTCC AACCTCAGCC AAGCTTCCAC CTTGCAGGCC
    AACCGAGAAG ATTCCATGAA TATCCTGGAC CCTGAGGATG AGGAGGAGCA CACTCAGGAA
    GAGGACAGCA GTGGCAGTAA CGAGGATGAG GATGATAGTC AGGATGAAGA GGAGGAGGAG
    GAGGAAGATG AGGAAGATGA TCAGGAGGAT GATGAAGGTG AAGAGGGAGA TGAAGACGAT
    GACGACGATG GCTCTGAAAT GGAATTGGAT GAGGATTACC CTGATATGAA CGCTTCTCCC
    TTGGTCCGAT TTGAGCGCTT TGACCGGGAG GATGATCTCA TCATTGAGTT TGACAACATG
    TTCTCCAGTG CTACAGACAT CCCCCCATCC CCAGGAAATA TCCCTACCAC CCACCCACTG
    ATGGTACGCC ATGCAGATCA CAGTTCCCTG ACACTGGGCA GTGGCTCCTC TACAACACGT
    CTCACCCAGG GCATTGGGCG CAGTCAAAGG ACCCTGAGAC AGCTGACAGC CAACACAGGC
    CATACCATTC ACGTTCACTA CCCTGGGAAT CGCCAGCCCA ATCCTCCTCT TATACTCCAG
    AGGTTGCTTG GTCCCTCTGC TGCTGCTGAC ATCCTTCAGC TGAGCAGCAG CCTTCCCCTA
    CAAAGCCGAG GCCGAGCCCG CCTCCTAGTG GGCAACGATG ACGTCCACAT CATCGCCCGG
    TCTGATGATG AACTACTGGA TGACTTTTTC CATGATCAGA GCACAGCTAC CAGCCAAGCA
    GGGACCCTAT CCAGTATCCC TACAGCCCTG ACTCGCTGGA CAGAGGAATG CAAAGTTCTT
    GATGCTGAGA GCATGCATGA CTGTGTTTCA GTTGTTAAAG TGCCCATTGT CAATCATCTG
    GAATTCCTGA GGGATGAGGA GCTGGAAGAA AGGAGGGAGA AAAGGAGGAA ACAACTAGCT
    GAGGAAGAAA CAAAGATAAC CGACAAAGGC AAAGAAGATA AGGAGAACAG GGATCAGAGT
    GCACAGTGTG CTGCATCTAA GACAAATGAC TCCACTGAAC AGAACCTCTC AGATGGGACT
    CCTATGCCTG ACAGCTACCC AGCAACCCCA TCTTCAACTG ATGTAGCTAC ATCTGAGCCC
    AGGGAGACCC TTGTCACTCT GCAGCCCTCC CAACAGCAAC CAACACTCCC AACCCCACCA
    GCCTTGGGAG AGATTCCTCA AGAGCTGCAG TCCCCAATTG GAGAAGGTGG GAGCTCTACA
    CAGCTATTGA TGCCTGTAGA GCCAGAGGAA TTGGGTCCCA CAAGGCCAAG TGGAGAAGCA
    GAAACAACTC AGATGGAGCT ATCTCCAGCT CCCACTATAA CCTCTCTTTC CCCAGAGAGA
    GCTGAAGATT CTGATGCTCT GACAGCTGTC AGCAGTCAAC TGGAAGGCTC TCCCATGGAT
    ACTAGCAGCC TGGCTTCCTG TACCCTGGAG GAGGCTGTGG GTGACGCCCC AGCAACTGGC
    AGTTCTGAGC AGCCCACAGC AGGCAGCTCC ACTCCTGGGG ATGCCCCACC AGTTGTGACA
    GAAGTACAAG GCAGGGGTGA TGGGTCAGGG GAACCTACCC AGCCCCCTGA GGACAGCTCT
    CCCCCTGCAT CCTCTGAGAG TTCTTCCACC AGAGATTCCG CGGTGGCCAT TTCTGGAGCA
    GATTCCCGGG GAATCCTGGA AGAGCCATTG CCTTCAACAA GCAGTGAAGA AGAAGATCCT
    CTTGCAGGTA TTAGTCTCCC CGAAGGTGTG GACCCCTCTT TTCTGGCCGC TCTGCCTGAT
    GACATTCGCC GAGAAGTCCT ACAGAACCAG CTGGGCATTC GTCCACCAAC ACGAACTGCC
    CCTTCAACAA ATAACTCAGC TCCTGCAGTG GTGGGGAATC CTGGTGTCAC CGAAGTGAGC
    CCTGAGTTCC TGGCTGCTCT GCCTCCAGCC ATCCAGGAGG AAGTACTGGC ACAGCAGAGA
    GCTGAGCAAC AGCGACGGGA ACTAGCACAA AATGCCAGCT CAGACACCCC CATGGACCCT
    GTGACCTTCA TCCAGACTCT GCCCTCAGAC CTGCGCCGTA GTGTCCTAGA GGACATGGAG
    GACAGTGTGT TGGCCGTGAT GCCACCTGAC ATCGCAGCTG AAGCTCAAGC CCTGAGGCGA
    GAGCAAGAAG CCCGGCAGAG GCAGCTCATG CATGAGCGAC TTTTTGGGCA CAGCAGCACC
    TCTGCACTCT CTGCCATTCT CCGAAGCCCA GCCTTCACCA GTCGCCTGAG TGGCAACCGT
    GGGGTCCAAT ACACTCGCCT TGCTGTGCAA AGAGGTGGCA CCTTCCAGAT GGGGGGTAGC
    AGCAGCCATA ATAGGCCTTC TGGCAGTAAT GTGGACACCC TCCTACGTCT TCGAGGACGG
    CTCCTCCTGG ATCACGAAGC CCTGTCTTGT CTCCTGGTCC TACTTTTTGT GGATGAGCCA
    AAACTCAATA CTAGCCGTCT TCACCGAGTA CTGAGAAATC TCTGCTACCA TGCCCAGACC
    CGCCACTGGG TTATCCGTAG CCTGCTTTCC ATCTTGCAGC GAAGCAGTGA GAGTGAGCTT
    TGCATTGAAA CACCCAAGCT CTCCTCAAGT GAGGAAAAGG GCAAAAAGTC AAGCAAGAGC
    TGTGGGTCAA GCAGCCATGA GAACCGGCCC CTGGACCTGC TACACAAGAT GGAGTCTAAG
    AGTTCCAACC AGCTTTCCTG GCTCTCAGTA TCCATGGATG CAGCCCTAGG CTGCAGGACT
    AATATATTCC AGATCCAGCG TTCAGGGGGT CGCAAACATA CTGAGAAACA TGCGAGTAGT
    GGCTCCACTG TCCATATCCA CCCCCAGGCT GCTCCTGTTG TCTGCAGACA TGTTCTGGAT
    ACACTCATTC AATTGGCCAA GGTGTTTCCC AGTCACTTCA CACAGCAGCG GACCAAAGAA
    ACAAACTGTG AGAGTGATCG GGAGAGGGGC AGTAAGGCCT GCAGCCCATG CTCTTCACAG
    TCCACAAGCA GTGGCATTTG CACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG
    AATGTCAGCC GGAAAGGCAA GAACTCCGTG AAGTCAGTGC CAGTGAGCGC TGGTGGTGAG
    GGGGAAACCT CCCCATACAG CCTCGAAGCT TCTCCACTGG GGCAACTCAT GAACATGTTG
    TCACACCCAG TCATCCGCCG GAGCTCTCTC TTAACTGAGA AGCTCCTCAG ACTTCTTTCT
    CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTGTCGGAAG CACAGGCTAA TTCTGGCAGC
    AGTGCTTCCT CCACCACTGT TGCCACTTCA ACCACATCTA CCACCACTAT CAGTGCCACC
    TCCAGCACAT CTACTCTGCC TGCTGCAACC ACCCCTGTCA CTTCTGTTCC AGCCCTGGTT
    GCTGCCACGG CTATATCCAC CATTGCCGTA GCCGCTTCAA CCACAGTGAC TACCCCCACA
    ACTGCTACCA CTACTGTTTC AACTGCTACT ACTACTAAGG CCAGCAAATC TCCAGCTAAA
    GTTGGTGATA TCGGCAGCAG CAGCAGCACA GACTTTAAGA TGGTATCCTC TGGCCTCACT
    GAAAATCAGC TCCAGCTCTC TGTGGAAGTG TTGACATCCC ATTCTTGTTC TGAAGAAGGC
    CTAGAAGATG CAGCAAATGT GTTACTGCAG CTCTCTCGGG GGGACCCTGG GACCCGGGAC
    ACTGTTTTGA AGTTGCTACT GAATGGAGCC CGCCATCTGG GTTATACGCT TTGTAAACAG
    ATAGGAACTC TTCTGGCTGA GCTACGGGAA TACAATCTGG AACAGCAGCG GCGAGCCCAG
    TGTGAAACCC TCTCCCCTGA TGGCATGCCT GAGGAACAGC CACAGACCAC CAAGCTGAAA
    GGCAAAATGC AGAGCAGGTT TGACGTGGCT GAGAATGTGG TAATCGTGGC ATCTCAGAAG
    CGGCCTCTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA
    TCTACCCAGA AGTTCTTCTT GAGGGTACTG CAGGTCATCA TCCAGCTCCG GGATGACACG
    CGCCGTGCTA ACAAGAAGGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA
    GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA
    ATGGTACGTG AGGGTCAGAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCAAGC
    CAGTCAGAGG CATCTGTCCG AAGGGAGGAG TCACCCATGG ATGTGGACCA GCCATCTCCC
    AGTGCTCAAG ATACTCAGTC CATTGGCTCA GATGGAACTC CACAGGGAGA GAAGGAGAAG
    GAGGAGAGGC CACCTGAGTT ACCCCTGCTC AGTGAGCAGC TGAACTTGGA TGAGCTGTGG
    GACATGCTGG GAGAGTGTCT AAAGGAACTG GAGGAATCCC ATGACCAGCA TGCGGTGCTA
    GTGCTACAAC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CAACAGAGCG GGAGAGCAAG
    CCTCCTGTCC GGGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCACCTCCA
    CTCTCCCCTG CCCCTTTAAC CCCAGCCACT CCTTCCTCCC TTGACCCGTT CTTCTCCCGG
    GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTTCTTCGT
    TTTGCAGAGA CTCATCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC CACCCACCTC
    GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT CGATGTCAAG
    CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTGGACGAGG GACTCCGGAA AGAAGACATG
    GCTGTGCACG TTCGTCGTGA TCATGTCTTT GAAGACTCCT ATCGTGAGCT GCACCGGAAA
    TCCCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT
    GCTGGAGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG
    TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGGGTCACAT ACACCATCAA TCCATCTTCT
    CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTTG GACGCATCGT GGCCAAAGCT
    GTGTATGACA ACCGCCTCCT GGAGTGCTAC TTTACTAGGT CCTTCTACAA ACACATTTTG
    GGCAAGTCTG TCAGATATAC AGATATGGAG AGTGAAGACT ACCACTTTTA CCAGGGCCTG
    GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ATCTCACTTT CAGCACTGAG
    GTCCAAGAGT TTGGAGTCTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG
    GTAACAGAGG AAAATAAGAA GGAATATGTA CACCTGGTGT GCCAGATGAG AATGACAGGA
    GCCATCCGCA AACAGCTGGC GGCTTTCTTA GAAGGTTTCT ATGAGATCAT TCCAAAGCGC
    CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTCA TATCAGGACT GCCCACAATC
    GACATTGACG ATTTGAAATC TAACACTGAA TATCACAAGT ACCAGTCCAA CTCTATTCAG
    ATCCAGTGGT TCTGGAGAGC ACTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC
    CAGTTTGTCA CAGGTACTTC CAAGGTGCCC CTGCAAGGTT TTGCTGCCCT TGAAGGCATG
    AATGGCATTC AGAAGTTTCA AATCCATCGA GATGACAGGT CCACAGACCG CCTGCCTTCA
    GCTCACACAT GTTTTAATCA GCTGGACCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC
    CACATGCTAC TATTGGCCAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35633
    Clone ID Related Accession (Same CDS sequence) XM_024987894.1 , XM_010821997.2 , XM_005228212.2 , XM_005228212.3 , XM_024987895.1
    Accession Version XM_024987895.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATAA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTCCACTT AGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGATTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT
    GTACTTGTGC GAAACTGTAT CCAGGCCATG ATTGACCCTT CCATGGATCC ATACCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CAAGCTACGA TGCTGGTGGC
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG CCGAAAAGAA TGCCCTTTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTGCACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TACAGATGTG
    ACTATGGAAA GTAGTCCCAG CTCATCCATC TCTATGGAGC ACCGGCTGGA TGTTGAATTA
    AGGGCATCAA GTTCCAGCAG CAGTAGCATC TCCTCTGGCC CTGGCCCTCG TCCTGGAGTC
    CAGTGTATTC CACAACGAGC CGCACTTCTC AAATCCATGT TGAATTTCCT CAAGAAGGCC
    ATTCAAGACC CTGCTTTCTC AGATGGCATA CGACATGTGA TGGATGGTTC TCTGCCTACC
    TCCCTGAAAC ACATCATCAG CAATGCAGAA TACTATGGCC CATCACTCTT TCTCCTAGCT
    ACTGAAGTGG TGACTGTGTT TGTATTTCAA GAACCATCAT TGCTTTCCTC ACTCCAGGAC
    AATGGATTGA CAGATGTCAT GCTACATGCA CTGCTTATCA AAGACGTTCC TGCTACCCGT
    GAAGTTCTTG GCTCCCTCCC AAATGTTTTC AGTGCACTCT GCTTGAATGC CCGAGGTCTG
    CAGTCATTTG TACAGTGTCA GCCTTTTGAA CGTCTCTTCA AAGTTCTCCT GTCTCCAGAT
    TACCTCCCAG CCATGCGGAG AAGGAGGAGT TCTGATCCTC TAGGGGATAC TGCCTCCAAC
    CTAGGAAGTG CTGTAGATGA GCTCATGAGA CATCAGCCTA CCCTCAAAAC AGATGCAACG
    ACTGCGATCA TCAAGTTACT TGAAGAAATC TGTAATCTTG GAAGAGACCC TAAATACATC
    TGTCAGAAGC CATCAATCCA GAAGGCAGAT GGCACTGCCG CTGCTCCTCC CCCAAGATCT
    AACCATGCTG CAGAAGAGGC CTCTAGTGAG GATGAGGAGG AAGAGGAAGT ACAGGCCATG
    CAGAGCTTTA ATTCTACCCA GCAGAATGAA ACTGAGCCTA ATCAGCAGGT TGTTGGTACA
    GAGGAGCGTA TTCCTATTCC CCTCATGGAT TACATCCTTA ATGTGATGAA ATTTGTGGAA
    TCTATTCTGA GCAACAATAC AACTGATGAC CACTGCCAGG AATTTGTGAA TCAGAAAGGA
    CTCTTGCCTT TGGTCACCAT TTTGGGTCTT CCCAATCTGC CCATTGACTT TCCCACGTCT
    GCAGCCTGTC AGGCTGTTGC AGGTGTCTGC AAATCCATAT TGACACTGTC ACATGAACCC
    AAAGTCCTCC AGGAAGGTCT CCTTCAGTTG GACTCCATTC TTTCCTCCTT GGAGCCCTTA
    CACCGCCCAA TAGAATCCCC CGGGGGCTCA GTGTTGTTGC GAGAACTGGC TTGTGCTGGC
    AATGTTGCTG ATGCTACCCT CTCAGCTCAA GCCACACCTC TACTACATGC ACTCACTGCT
    GCCCATGCTT ACATCATGAT GTTTGTTCAT ACTTGCAGGG TTGGACAGAG TGAAATTCGT
    TCCATCTCCA TAAACCAGTG GGGCTCTCAG TTGGGTCTGA GTGTTCTGAG CAAGCTGAGC
    CAGTTATACT GTTCCCTGGT ATGGGAAAGC ACTGTCCTCC TCTCCCTCTG TACCCCAAAC
    AGCTTGCCAT CTGGGTGTGA ATTTGGCCAG GCAGACATGC AGAAACTGGT TCCAAAGGAT
    GAGAAGACAG GTACGACCCA GGGCGGAAAA AGATCAGATG GGGATCAGGA TGGAACCACT
    GGAAGTATGG ATGCTTCTAC CCAGGGCTTG TTGGAAGGCA TTGGGCTTGA TGGTGACACA
    CTGGCTCCTA TGGAGACAGA CGAACCTACT GCTTCAGACT CTAAGGGCAA ATCTAAAATC
    ACACCTGCCA TGGCTGCCAG AATCAAGCAA ATCAAGCCAT TGTTGTCAGC TTCCTCTAGA
    TTAGGCAGAG CACTGGCTGA GCTGTTTGGA CTCCTTGTTA AACTTTGTGT GGGGTCTCCT
    GTCCGCCAGA GAAGAAGCCA TCATGCTGCC AGCACCACCA CAGCACCAAC ACCTGCTGCT
    CGATCAACGG CCTCAGCCCT CACTAAGCTC CTCACAAAAG GCTTATCATG GCAGCCTCCA
    CCATATACGC CTACTCCACG CTTCAGGCTG ACCTTCTTCA TCTGTTCAGT TGGTTTTACA
    TCCCCAATGC TGTTTGATGA GAGGAAGTAT CCCTACCACC TCATGCTGCA GAAATTTCTC
    TGCTCTGGAG GCCACAATGC CCTTTTTGAA ACATTCAACT GGGCTCTGTC TATGGGAGGA
    AAAGTTCCTG TTGCTGAGGG ATTAGAACAT TCAGACTTAC CTGATGGCAC AGGAGAATTC
    CTAGATGCCT GGCTAATGCT GGTGGAGAAG ATGGTAAATC CCACCACTGT GCTTGAATCT
    CCACATTCAC TGCCTGCCAA ATTGCCTGGA GGTGTTCAGA ACTTTCCACA GTTCAGTGCC
    CTCCGCTTCC TTGTGGTAAC TCAGAAGGCA GCATTTACTT GCATCAAGAA CCTGTGGAAC
    CGGAAACCCT TGAAAGTGTA TGGTGGGCGA ATGGCTGAGT CCATGCTAGC CATCCTGTGC
    CACATCCTCC GAGGAGAACC TGTGATTCGA GAGAGACTGA GCAAAGAAAA GGAGGGATCT
    CGAGGAGAAG AGGATACAGG ACAAGAAGAA GGTGGCTCTC GCCGGGAACC TCAAGTCAAC
    CAGCAACAAC TACAACAGCT CATGGACATG GGCTTCACAA GGGAACATGC CATGGAGGCC
    CTGTTGAACA CCAGTACCAT GGAGCAGGCC ACAGAGTACC TTCTAACCCA CCCTCCCCCG
    ATCATGGGAG GAGTTGTTCG GGATCTGAGT ATGTCTGAAG AGGACCAGAT GATGAGAGCG
    ATTGCCATGT CTCTGGGACA GGATATCCCA ATGGATCAAA GAGCAGAGTC ACCTGAGGAA
    GTTGCTTGCC GAAAGGAAGA GGAGGAACGG AAAGCTCGAG AAAAGCAGGA AGAGGAAGAG
    GCTAAATGTC TAGAGAAGTT CCAGGATGCT GATCCATTGG AGCAAGATGA GCTCCACACT
    TTCACAGATA CAATGTTGCC AGGCTGCTTT CACCTTCTTG ATGAGTTGCC AGACACTGTA
    TACCGAGTGT GTGATCTGAT CATGACAGCA ATCAAACGAA ATGGAGCAGA CTATCGAGAC
    ATGATTCTGA AGCAAGTAGT TAATCAGGTG TGGGAAGCTG CTGATGTATT GATCAAAGCT
    GCTCTTCCCC TGACAACAAG TGACACAAAA ACTGTATCAG AGTGGATCAG TCAGATGGCC
    ACTCTACCCC AGGCCTCTAA TTTGGCCACT AGAATCTTGC TTTTAACACT GCTTTTTGAG
    GAATTGAAGC TACCTTGTGC TTGGGTGGTT GAATCTAGTG GCATCCTTAA TGTCCTAATC
    AAACTCTTGG AAGTGGTTCA GCCCTGCCTA CAGGCCGCCA AGGAACAAAA GGAGGTCCAG
    ACCCCAAAGT GGATCACTCC AGTGTTGCTC CTGATTGATT TCTATGAAAA AACAGCCATT
    TCCTCAAAGA GGAGAGCCCA AATGACTAAG TATCTACAGT CTAACAACAA CAACTGGCGC
    TGGTTTGATG ACCGCTCTGG ACGTTGGTGT AGTTACAGTG CAAGCAACAA CAGCACTATT
    GATTCTGCCT GGAAATCTGG AGAGACAAGT GTGCGTTTCA CTGCAGGCCG AAGAAGATAC
    ACTGTCCAGT TCACTACAAT GGTACAGGTT AATGAGGAAA CAGGGAACCG GCGTCCTGTG
    ATGCTGACAC TCCTGAGGGT ACCCCGATTG AATAAAAATT CTAAAAACAG CAATGGACAG
    GAACTAGAGA AGACACTGGA AGAAAGCAAA GAAATGGATA TCAAACGTAA AGAAAATAAA
    ACCAGTGATA CCCCCTTGGC CCTAGACAGT ACAAATACTG AAAAGGAGAC AAGCCTGGAA
    GAAACAAAAA TTGGGGAGAT CTTGATCCAG GGCCTGACAG AGGATATGGT GACTGTTTTA
    ATCCGGGCCT GTGTGAGCAT GCTAGGCGTC CCTGTGGATC CAGACACTTT GCATGCCACC
    CTTCGCCTAT GCCTGAGGCT CACCCGAGAC CACAAATATG CCATGATGTT TGCAGAGCTG
    AAGAGTACTC GCATGATCTT AAATTTGACC CAGAGTTCAG GCTTCAATGG GTTTACTCCC
    CTGGTTACCC TTCTCTTAAG ACACATCATT GAGGACCCCT GCACCCTGCG CCATACCATG
    GAAAAGGTTG TTCGCTCAGC AGCTACAAGT GGAGCTGGTA GCACTACTTC TGGTGTTGTA
    TCTGGCAGCC TTGGCTCTCG GGAGATCAAC TACATCCTTC GTGTCCTTGG ACCAGCCGCA
    TGCCGCAACC CAGACATTTT CACAGAAGTG GCCAACTGCT GTATCCGCAT TGCCCTTCCA
    GCCCCTCGCG GCTCAGGAAC TGCTTCAGAT GATGAGTTTG AGAATCTCAG AATTAAAGGC
    CCGAATGCTG TACAGCTGGT GAAAACAACC CCTTTGAAGC CCTCACCTCT GCCTGTCATT
    CCTGATACTA TCAAGGAAGT GATCTATGAC ATGCTAAATG CCCTGGCTGC ATACCATGCT
    CCAGAGGAAG CAGACAAATC TGATCCTAAA CCAGGGGGTA TGACTCAAGA GGTTGGCCAG
    CTCCTGCAAG ACATGGGTGA TGACGTATAC CAGCAGTACC GGTCACTTAC CCGTCAGAGC
    AGTGACTTTG ATGCACAGAC AGGATTTTCC ATTAATAGTC AGGTCTTTGC TGCAGATGGT
    TCCTCCACTG AGACTTCCGC ATCTGGGGTA TCCCAAGGAG AAGTGTCAAC TCCGGAGGAG
    TCTCGGGATG GGAAGAAAGA TAAAGAAGGG GACCGGGCCT CTGAGGAAGG CAAGCAGAAA
    GGCAAGGGCA GCAAACCTTT AATGCCCACC TCCACTATTC TTCGGCTTCT GGCAGAGTTG
    GTGAGATCCT ATGTTGGTAT TGCTACCCTG ATTGCCAACT ACAGCTACAC TGTGGGCCAG
    TCTGAGCTGA TCAAAGAGGA CTGCAGTGTG CTAGCTTTTG TCTTGGACCA CCTCCTCCCG
    CATACCCAGA ATGCAGAAGA CAAAGACACA CCGGCGCTGG CCCGCCTGTT CCTCGCAAGC
    CTGGCTGCCT CAGGGAGTGG CACAGATGCC CAGGTGGCCC TTGTGAATGA AGTAAAAGCA
    GCCCTGGGAA GGGCACTGGC TATGGCTGAG AGTACAGAGA AACATGCCAG ACTTCAGGCA
    GTGATGTGTA TCATCAGTAC TATCATGGAG TCCTGCCCCT CCACCTCCAG TTTCTACAGC
    AGTGCAACAG CCAAGACCCA ACACAACGGC ATGAACAACA TCATTCGACT CTTCCTGAAG
    AAGGGACTGG TTAATGACCT GGCCAGAGTG CCTCACAGCC TGGACCTGTC CAGTCCCAAC
    ATGGCCAACA CTGTCAATGC TGCTCTGAAG CCTTTGGAAA CACTTTCCCG GATTGTGAAC
    CAGCCCAGTA GCCTTTTTGG CAGTAAGAGT GCTTCTAGCA AGAGCAAGTC TGAGCAGGAT
    GCCCAAGGAG CTGCTCAGGA CTCTAATAGC AACCAACAGG ACCCAGGCGA GCCTGGGGAA
    ACAGAAGTGC AGGAGGAGGA TCATGATGTC ACTCAGACAG AGGTGGCAGA TGGGGATATC
    ATGGATGGGG AGGCTGAAAC CGACTCAGTG GTGATTGCTG GGCAGCCTGA GGTGCTCAGT
    TCACAAGAGA TGCAGGTTGA GAATGAGCTG GAGGACCTGA TAGATGAGTT GCTTGAGAGG
    GATGGCGGAT CTGGGAACAG TACAATTATA GTGAGCAGAA GTGGAGAGGA TGAATCACAA
    GAGGACGTGC TGATGGATGA AGCTCCTTCC AACCTCAGCC AAGCTTCCAC CTTGCAGGCC
    AACCGAGAAG ATTCCATGAA TATCCTGGAC CCTGAGGATG AGGAGGAGCA CACTCAGGAA
    GAGGACAGCA GTGGCAGTAA CGAGGATGAG GATGATAGTC AGGATGAAGA GGAGGAGGAG
    GAGGAAGATG AGGAAGATGA TCAGGAGGAT GATGAAGGTG AAGAGGGAGA TGAAGACGAT
    GACGACGATG GCTCTGAAAT GGAATTGGAT GAGGATTACC CTGATATGAA CGCTTCTCCC
    TTGGTCCGAT TTGAGCGCTT TGACCGGGAG GATGATCTCA TCATTGAGTT TGACAACATG
    TTCTCCAGTG CTACAGACAT CCCCCCATCC CCAGGAAATA TCCCTACCAC CCACCCACTG
    ATGGTACGCC ATGCAGATCA CAGTTCCCTG ACACTGGGCA GTGGCTCCTC TACAACACGT
    CTCACCCAGG GCATTGGGCG CAGTCAAAGG ACCCTGAGAC AGCTGACAGC CAACACAGGC
    CATACCATTC ACGTTCACTA CCCTGGGAAT CGCCAGCCCA ATCCTCCTCT TATACTCCAG
    AGGTTGCTTG GTCCCTCTGC TGCTGCTGAC ATCCTTCAGC TGAGCAGCAG CCTTCCCCTA
    CAAAGCCGAG GCCGAGCCCG CCTCCTAGTG GGCAACGATG ACGTCCACAT CATCGCCCGG
    TCTGATGATG AACTACTGGA TGACTTTTTC CATGATCAGA GCACAGCTAC CAGCCAAGCA
    GGGACCCTAT CCAGTATCCC TACAGCCCTG ACTCGCTGGA CAGAGGAATG CAAAGTTCTT
    GATGCTGAGA GCATGCATGA CTGTGTTTCA GTTGTTAAAG TGCCCATTGT CAATCATCTG
    GAATTCCTGA GGGATGAGGA GCTGGAAGAA AGGAGGGAGA AAAGGAGGAA ACAACTAGCT
    GAGGAAGAAA CAAAGATAAC CGACAAAGGC AAAGAAGATA AGGAGAACAG GGATCAGAGT
    GCACAGTGTG CTGCATCTAA GACAAATGAC TCCACTGAAC AGAACCTCTC AGATGGGACT
    CCTATGCCTG ACAGCTACCC AGCAACCCCA TCTTCAACTG ATGTAGCTAC ATCTGAGCCC
    AGGGAGACCC TTGTCACTCT GCAGCCCTCC CAACAGCAAC CAACACTCCC AACCCCACCA
    GCCTTGGGAG AGATTCCTCA AGAGCTGCAG TCCCCAATTG GAGAAGGTGG GAGCTCTACA
    CAGCTATTGA TGCCTGTAGA GCCAGAGGAA TTGGGTCCCA CAAGGCCAAG TGGAGAAGCA
    GAAACAACTC AGATGGAGCT ATCTCCAGCT CCCACTATAA CCTCTCTTTC CCCAGAGAGA
    GCTGAAGATT CTGATGCTCT GACAGCTGTC AGCAGTCAAC TGGAAGGCTC TCCCATGGAT
    ACTAGCAGCC TGGCTTCCTG TACCCTGGAG GAGGCTGTGG GTGACGCCCC AGCAACTGGC
    AGTTCTGAGC AGCCCACAGC AGGCAGCTCC ACTCCTGGGG ATGCCCCACC AGTTGTGACA
    GAAGTACAAG GCAGGGGTGA TGGGTCAGGG GAACCTACCC AGCCCCCTGA GGACAGCTCT
    CCCCCTGCAT CCTCTGAGAG TTCTTCCACC AGAGATTCCG CGGTGGCCAT TTCTGGAGCA
    GATTCCCGGG GAATCCTGGA AGAGCCATTG CCTTCAACAA GCAGTGAAGA AGAAGATCCT
    CTTGCAGGTA TTAGTCTCCC CGAAGGTGTG GACCCCTCTT TTCTGGCCGC TCTGCCTGAT
    GACATTCGCC GAGAAGTCCT ACAGAACCAG CTGGGCATTC GTCCACCAAC ACGAACTGCC
    CCTTCAACAA ATAACTCAGC TCCTGCAGTG GTGGGGAATC CTGGTGTCAC CGAAGTGAGC
    CCTGAGTTCC TGGCTGCTCT GCCTCCAGCC ATCCAGGAGG AAGTACTGGC ACAGCAGAGA
    GCTGAGCAAC AGCGACGGGA ACTAGCACAA AATGCCAGCT CAGACACCCC CATGGACCCT
    GTGACCTTCA TCCAGACTCT GCCCTCAGAC CTGCGCCGTA GTGTCCTAGA GGACATGGAG
    GACAGTGTGT TGGCCGTGAT GCCACCTGAC ATCGCAGCTG AAGCTCAAGC CCTGAGGCGA
    GAGCAAGAAG CCCGGCAGAG GCAGCTCATG CATGAGCGAC TTTTTGGGCA CAGCAGCACC
    TCTGCACTCT CTGCCATTCT CCGAAGCCCA GCCTTCACCA GTCGCCTGAG TGGCAACCGT
    GGGGTCCAAT ACACTCGCCT TGCTGTGCAA AGAGGTGGCA CCTTCCAGAT GGGGGGTAGC
    AGCAGCCATA ATAGGCCTTC TGGCAGTAAT GTGGACACCC TCCTACGTCT TCGAGGACGG
    CTCCTCCTGG ATCACGAAGC CCTGTCTTGT CTCCTGGTCC TACTTTTTGT GGATGAGCCA
    AAACTCAATA CTAGCCGTCT TCACCGAGTA CTGAGAAATC TCTGCTACCA TGCCCAGACC
    CGCCACTGGG TTATCCGTAG CCTGCTTTCC ATCTTGCAGC GAAGCAGTGA GAGTGAGCTT
    TGCATTGAAA CACCCAAGCT CTCCTCAAGT GAGGAAAAGG GCAAAAAGTC AAGCAAGAGC
    TGTGGGTCAA GCAGCCATGA GAACCGGCCC CTGGACCTGC TACACAAGAT GGAGTCTAAG
    AGTTCCAACC AGCTTTCCTG GCTCTCAGTA TCCATGGATG CAGCCCTAGG CTGCAGGACT
    AATATATTCC AGATCCAGCG TTCAGGGGGT CGCAAACATA CTGAGAAACA TGCGAGTAGT
    GGCTCCACTG TCCATATCCA CCCCCAGGCT GCTCCTGTTG TCTGCAGACA TGTTCTGGAT
    ACACTCATTC AATTGGCCAA GGTGTTTCCC AGTCACTTCA CACAGCAGCG GACCAAAGAA
    ACAAACTGTG AGAGTGATCG GGAGAGGGGC AGTAAGGCCT GCAGCCCATG CTCTTCACAG
    TCCACAAGCA GTGGCATTTG CACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG
    AATGTCAGCC GGAAAGGCAA GAACTCCGTG AAGTCAGTGC CAGTGAGCGC TGGTGGTGAG
    GGGGAAACCT CCCCATACAG CCTCGAAGCT TCTCCACTGG GGCAACTCAT GAACATGTTG
    TCACACCCAG TCATCCGCCG GAGCTCTCTC TTAACTGAGA AGCTCCTCAG ACTTCTTTCT
    CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTGTCGGAAG CACAGGCTAA TTCTGGCAGC
    AGTGCTTCCT CCACCACTGT TGCCACTTCA ACCACATCTA CCACCACTAT CAGTGCCACC
    TCCAGCACAT CTACTCTGCC TGCTGCAACC ACCCCTGTCA CTTCTGTTCC AGCCCTGGTT
    GCTGCCACGG CTATATCCAC CATTGCCGTA GCCGCTTCAA CCACAGTGAC TACCCCCACA
    ACTGCTACCA CTACTGTTTC AACTGCTACT ACTACTAAGG CCAGCAAATC TCCAGCTAAA
    GTTGGTGATA TCGGCAGCAG CAGCAGCACA GACTTTAAGA TGGTATCCTC TGGCCTCACT
    GAAAATCAGC TCCAGCTCTC TGTGGAAGTG TTGACATCCC ATTCTTGTTC TGAAGAAGGC
    CTAGAAGATG CAGCAAATGT GTTACTGCAG CTCTCTCGGG GGGACCCTGG GACCCGGGAC
    ACTGTTTTGA AGTTGCTACT GAATGGAGCC CGCCATCTGG GTTATACGCT TTGTAAACAG
    ATAGGAACTC TTCTGGCTGA GCTACGGGAA TACAATCTGG AACAGCAGCG GCGAGCCCAG
    TGTGAAACCC TCTCCCCTGA TGGCATGCCT GAGGAACAGC CACAGACCAC CAAGCTGAAA
    GGCAAAATGC AGAGCAGGTT TGACGTGGCT GAGAATGTGG TAATCGTGGC ATCTCAGAAG
    CGGCCTCTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA
    TCTACCCAGA AGTTCTTCTT GAGGGTACTG CAGGTCATCA TCCAGCTCCG GGATGACACG
    CGCCGTGCTA ACAAGAAGGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA
    GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA
    ATGGTACGTG AGGGTCAGAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCAAGC
    CAGTCAGAGG CATCTGTCCG AAGGGAGGAG TCACCCATGG ATGTGGACCA GCCATCTCCC
    AGTGCTCAAG ATACTCAGTC CATTGGCTCA GATGGAACTC CACAGGGAGA GAAGGAGAAG
    GAGGAGAGGC CACCTGAGTT ACCCCTGCTC AGTGAGCAGC TGAACTTGGA TGAGCTGTGG
    GACATGCTGG GAGAGTGTCT AAAGGAACTG GAGGAATCCC ATGACCAGCA TGCGGTGCTA
    GTGCTACAAC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CAACAGAGCG GGAGAGCAAG
    CCTCCTGTCC GGGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCACCTCCA
    CTCTCCCCTG CCCCTTTAAC CCCAGCCACT CCTTCCTCCC TTGACCCGTT CTTCTCCCGG
    GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTTCTTCGT
    TTTGCAGAGA CTCATCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC CACCCACCTC
    GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT CGATGTCAAG
    CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTGGACGAGG GACTCCGGAA AGAAGACATG
    GCTGTGCACG TTCGTCGTGA TCATGTCTTT GAAGACTCCT ATCGTGAGCT GCACCGGAAA
    TCCCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT
    GCTGGAGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG
    TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGGGTCACAT ACACCATCAA TCCATCTTCT
    CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTTG GACGCATCGT GGCCAAAGCT
    GTGTATGACA ACCGCCTCCT GGAGTGCTAC TTTACTAGGT CCTTCTACAA ACACATTTTG
    GGCAAGTCTG TCAGATATAC AGATATGGAG AGTGAAGACT ACCACTTTTA CCAGGGCCTG
    GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ATCTCACTTT CAGCACTGAG
    GTCCAAGAGT TTGGAGTCTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG
    GTAACAGAGG AAAATAAGAA GGAATATGTA CACCTGGTGT GCCAGATGAG AATGACAGGA
    GCCATCCGCA AACAGCTGGC GGCTTTCTTA GAAGGTTTCT ATGAGATCAT TCCAAAGCGC
    CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTCA TATCAGGACT GCCCACAATC
    GACATTGACG ATTTGAAATC TAACACTGAA TATCACAAGT ACCAGTCCAA CTCTATTCAG
    ATCCAGTGGT TCTGGAGAGC ACTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC
    CAGTTTGTCA CAGGTACTTC CAAGGTGCCC CTGCAAGGTT TTGCTGCCCT TGAAGGCATG
    AATGGCATTC AGAAGTTTCA AATCCATCGA GATGACAGGT CCACAGACCG CCTGCCTTCA
    GCTCACACAT GTTTTAATCA GCTGGACCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC
    CACATGCTAC TATTGGCCAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843663.1
    CDS242..13495
    Translation

    Target ORF information:

    RefSeq Version XM_024987895.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987895.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATAA AAGCTGCTTC TTTACGAACG TTAACATCAA TTGTCCACTT AGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGATTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCT
    GTACTTGTGC GAAACTGTAT CCAGGCCATG ATTGACCCTT CCATGGATCC ATACCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CAAGCTACGA TGCTGGTGGC
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCTGTCA GAGTGGTTGA CCTTATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG CCGAAAAGAA TGCCCTTTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTGCACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TACAGATGTG
    ACTATGGAAA GTAGTCCCAG CTCATCCATC TCTATGGAGC ACCGGCTGGA TGTTGAATTA
    AGGGCATCAA GTTCCAGCAG CAGTAGCATC TCCTCTGGCC CTGGCCCTCG TCCTGGAGTC
    CAGTGTATTC CACAACGAGC CGCACTTCTC AAATCCATGT TGAATTTCCT CAAGAAGGCC
    ATTCAAGACC CTGCTTTCTC AGATGGCATA CGACATGTGA TGGATGGTTC TCTGCCTACC
    TCCCTGAAAC ACATCATCAG CAATGCAGAA TACTATGGCC CATCACTCTT TCTCCTAGCT
    ACTGAAGTGG TGACTGTGTT TGTATTTCAA GAACCATCAT TGCTTTCCTC ACTCCAGGAC
    AATGGATTGA CAGATGTCAT GCTACATGCA CTGCTTATCA AAGACGTTCC TGCTACCCGT
    GAAGTTCTTG GCTCCCTCCC AAATGTTTTC AGTGCACTCT GCTTGAATGC CCGAGGTCTG
    CAGTCATTTG TACAGTGTCA GCCTTTTGAA CGTCTCTTCA AAGTTCTCCT GTCTCCAGAT
    TACCTCCCAG CCATGCGGAG AAGGAGGAGT TCTGATCCTC TAGGGGATAC TGCCTCCAAC
    CTAGGAAGTG CTGTAGATGA GCTCATGAGA CATCAGCCTA CCCTCAAAAC AGATGCAACG
    ACTGCGATCA TCAAGTTACT TGAAGAAATC TGTAATCTTG GAAGAGACCC TAAATACATC
    TGTCAGAAGC CATCAATCCA GAAGGCAGAT GGCACTGCCG CTGCTCCTCC CCCAAGATCT
    AACCATGCTG CAGAAGAGGC CTCTAGTGAG GATGAGGAGG AAGAGGAAGT ACAGGCCATG
    CAGAGCTTTA ATTCTACCCA GCAGAATGAA ACTGAGCCTA ATCAGCAGGT TGTTGGTACA
    GAGGAGCGTA TTCCTATTCC CCTCATGGAT TACATCCTTA ATGTGATGAA ATTTGTGGAA
    TCTATTCTGA GCAACAATAC AACTGATGAC CACTGCCAGG AATTTGTGAA TCAGAAAGGA
    CTCTTGCCTT TGGTCACCAT TTTGGGTCTT CCCAATCTGC CCATTGACTT TCCCACGTCT
    GCAGCCTGTC AGGCTGTTGC AGGTGTCTGC AAATCCATAT TGACACTGTC ACATGAACCC
    AAAGTCCTCC AGGAAGGTCT CCTTCAGTTG GACTCCATTC TTTCCTCCTT GGAGCCCTTA
    CACCGCCCAA TAGAATCCCC CGGGGGCTCA GTGTTGTTGC GAGAACTGGC TTGTGCTGGC
    AATGTTGCTG ATGCTACCCT CTCAGCTCAA GCCACACCTC TACTACATGC ACTCACTGCT
    GCCCATGCTT ACATCATGAT GTTTGTTCAT ACTTGCAGGG TTGGACAGAG TGAAATTCGT
    TCCATCTCCA TAAACCAGTG GGGCTCTCAG TTGGGTCTGA GTGTTCTGAG CAAGCTGAGC
    CAGTTATACT GTTCCCTGGT ATGGGAAAGC ACTGTCCTCC TCTCCCTCTG TACCCCAAAC
    AGCTTGCCAT CTGGGTGTGA ATTTGGCCAG GCAGACATGC AGAAACTGGT TCCAAAGGAT
    GAGAAGACAG GTACGACCCA GGGCGGAAAA AGATCAGATG GGGATCAGGA TGGAACCACT
    GGAAGTATGG ATGCTTCTAC CCAGGGCTTG TTGGAAGGCA TTGGGCTTGA TGGTGACACA
    CTGGCTCCTA TGGAGACAGA CGAACCTACT GCTTCAGACT CTAAGGGCAA ATCTAAAATC
    ACACCTGCCA TGGCTGCCAG AATCAAGCAA ATCAAGCCAT TGTTGTCAGC TTCCTCTAGA
    TTAGGCAGAG CACTGGCTGA GCTGTTTGGA CTCCTTGTTA AACTTTGTGT GGGGTCTCCT
    GTCCGCCAGA GAAGAAGCCA TCATGCTGCC AGCACCACCA CAGCACCAAC ACCTGCTGCT
    CGATCAACGG CCTCAGCCCT CACTAAGCTC CTCACAAAAG GCTTATCATG GCAGCCTCCA
    CCATATACGC CTACTCCACG CTTCAGGCTG ACCTTCTTCA TCTGTTCAGT TGGTTTTACA
    TCCCCAATGC TGTTTGATGA GAGGAAGTAT CCCTACCACC TCATGCTGCA GAAATTTCTC
    TGCTCTGGAG GCCACAATGC CCTTTTTGAA ACATTCAACT GGGCTCTGTC TATGGGAGGA
    AAAGTTCCTG TTGCTGAGGG ATTAGAACAT TCAGACTTAC CTGATGGCAC AGGAGAATTC
    CTAGATGCCT GGCTAATGCT GGTGGAGAAG ATGGTAAATC CCACCACTGT GCTTGAATCT
    CCACATTCAC TGCCTGCCAA ATTGCCTGGA GGTGTTCAGA ACTTTCCACA GTTCAGTGCC
    CTCCGCTTCC TTGTGGTAAC TCAGAAGGCA GCATTTACTT GCATCAAGAA CCTGTGGAAC
    CGGAAACCCT TGAAAGTGTA TGGTGGGCGA ATGGCTGAGT CCATGCTAGC CATCCTGTGC
    CACATCCTCC GAGGAGAACC TGTGATTCGA GAGAGACTGA GCAAAGAAAA GGAGGGATCT
    CGAGGAGAAG AGGATACAGG ACAAGAAGAA GGTGGCTCTC GCCGGGAACC TCAAGTCAAC
    CAGCAACAAC TACAACAGCT CATGGACATG GGCTTCACAA GGGAACATGC CATGGAGGCC
    CTGTTGAACA CCAGTACCAT GGAGCAGGCC ACAGAGTACC TTCTAACCCA CCCTCCCCCG
    ATCATGGGAG GAGTTGTTCG GGATCTGAGT ATGTCTGAAG AGGACCAGAT GATGAGAGCG
    ATTGCCATGT CTCTGGGACA GGATATCCCA ATGGATCAAA GAGCAGAGTC ACCTGAGGAA
    GTTGCTTGCC GAAAGGAAGA GGAGGAACGG AAAGCTCGAG AAAAGCAGGA AGAGGAAGAG
    GCTAAATGTC TAGAGAAGTT CCAGGATGCT GATCCATTGG AGCAAGATGA GCTCCACACT
    TTCACAGATA CAATGTTGCC AGGCTGCTTT CACCTTCTTG ATGAGTTGCC AGACACTGTA
    TACCGAGTGT GTGATCTGAT CATGACAGCA ATCAAACGAA ATGGAGCAGA CTATCGAGAC
    ATGATTCTGA AGCAAGTAGT TAATCAGGTG TGGGAAGCTG CTGATGTATT GATCAAAGCT
    GCTCTTCCCC TGACAACAAG TGACACAAAA ACTGTATCAG AGTGGATCAG TCAGATGGCC
    ACTCTACCCC AGGCCTCTAA TTTGGCCACT AGAATCTTGC TTTTAACACT GCTTTTTGAG
    GAATTGAAGC TACCTTGTGC TTGGGTGGTT GAATCTAGTG GCATCCTTAA TGTCCTAATC
    AAACTCTTGG AAGTGGTTCA GCCCTGCCTA CAGGCCGCCA AGGAACAAAA GGAGGTCCAG
    ACCCCAAAGT GGATCACTCC AGTGTTGCTC CTGATTGATT TCTATGAAAA AACAGCCATT
    TCCTCAAAGA GGAGAGCCCA AATGACTAAG TATCTACAGT CTAACAACAA CAACTGGCGC
    TGGTTTGATG ACCGCTCTGG ACGTTGGTGT AGTTACAGTG CAAGCAACAA CAGCACTATT
    GATTCTGCCT GGAAATCTGG AGAGACAAGT GTGCGTTTCA CTGCAGGCCG AAGAAGATAC
    ACTGTCCAGT TCACTACAAT GGTACAGGTT AATGAGGAAA CAGGGAACCG GCGTCCTGTG
    ATGCTGACAC TCCTGAGGGT ACCCCGATTG AATAAAAATT CTAAAAACAG CAATGGACAG
    GAACTAGAGA AGACACTGGA AGAAAGCAAA GAAATGGATA TCAAACGTAA AGAAAATAAA
    ACCAGTGATA CCCCCTTGGC CCTAGACAGT ACAAATACTG AAAAGGAGAC AAGCCTGGAA
    GAAACAAAAA TTGGGGAGAT CTTGATCCAG GGCCTGACAG AGGATATGGT GACTGTTTTA
    ATCCGGGCCT GTGTGAGCAT GCTAGGCGTC CCTGTGGATC CAGACACTTT GCATGCCACC
    CTTCGCCTAT GCCTGAGGCT CACCCGAGAC CACAAATATG CCATGATGTT TGCAGAGCTG
    AAGAGTACTC GCATGATCTT AAATTTGACC CAGAGTTCAG GCTTCAATGG GTTTACTCCC
    CTGGTTACCC TTCTCTTAAG ACACATCATT GAGGACCCCT GCACCCTGCG CCATACCATG
    GAAAAGGTTG TTCGCTCAGC AGCTACAAGT GGAGCTGGTA GCACTACTTC TGGTGTTGTA
    TCTGGCAGCC TTGGCTCTCG GGAGATCAAC TACATCCTTC GTGTCCTTGG ACCAGCCGCA
    TGCCGCAACC CAGACATTTT CACAGAAGTG GCCAACTGCT GTATCCGCAT TGCCCTTCCA
    GCCCCTCGCG GCTCAGGAAC TGCTTCAGAT GATGAGTTTG AGAATCTCAG AATTAAAGGC
    CCGAATGCTG TACAGCTGGT GAAAACAACC CCTTTGAAGC CCTCACCTCT GCCTGTCATT
    CCTGATACTA TCAAGGAAGT GATCTATGAC ATGCTAAATG CCCTGGCTGC ATACCATGCT
    CCAGAGGAAG CAGACAAATC TGATCCTAAA CCAGGGGGTA TGACTCAAGA GGTTGGCCAG
    CTCCTGCAAG ACATGGGTGA TGACGTATAC CAGCAGTACC GGTCACTTAC CCGTCAGAGC
    AGTGACTTTG ATGCACAGAC AGGATTTTCC ATTAATAGTC AGGTCTTTGC TGCAGATGGT
    TCCTCCACTG AGACTTCCGC ATCTGGGGTA TCCCAAGGAG AAGTGTCAAC TCCGGAGGAG
    TCTCGGGATG GGAAGAAAGA TAAAGAAGGG GACCGGGCCT CTGAGGAAGG CAAGCAGAAA
    GGCAAGGGCA GCAAACCTTT AATGCCCACC TCCACTATTC TTCGGCTTCT GGCAGAGTTG
    GTGAGATCCT ATGTTGGTAT TGCTACCCTG ATTGCCAACT ACAGCTACAC TGTGGGCCAG
    TCTGAGCTGA TCAAAGAGGA CTGCAGTGTG CTAGCTTTTG TCTTGGACCA CCTCCTCCCG
    CATACCCAGA ATGCAGAAGA CAAAGACACA CCGGCGCTGG CCCGCCTGTT CCTCGCAAGC
    CTGGCTGCCT CAGGGAGTGG CACAGATGCC CAGGTGGCCC TTGTGAATGA AGTAAAAGCA
    GCCCTGGGAA GGGCACTGGC TATGGCTGAG AGTACAGAGA AACATGCCAG ACTTCAGGCA
    GTGATGTGTA TCATCAGTAC TATCATGGAG TCCTGCCCCT CCACCTCCAG TTTCTACAGC
    AGTGCAACAG CCAAGACCCA ACACAACGGC ATGAACAACA TCATTCGACT CTTCCTGAAG
    AAGGGACTGG TTAATGACCT GGCCAGAGTG CCTCACAGCC TGGACCTGTC CAGTCCCAAC
    ATGGCCAACA CTGTCAATGC TGCTCTGAAG CCTTTGGAAA CACTTTCCCG GATTGTGAAC
    CAGCCCAGTA GCCTTTTTGG CAGTAAGAGT GCTTCTAGCA AGAGCAAGTC TGAGCAGGAT
    GCCCAAGGAG CTGCTCAGGA CTCTAATAGC AACCAACAGG ACCCAGGCGA GCCTGGGGAA
    ACAGAAGTGC AGGAGGAGGA TCATGATGTC ACTCAGACAG AGGTGGCAGA TGGGGATATC
    ATGGATGGGG AGGCTGAAAC CGACTCAGTG GTGATTGCTG GGCAGCCTGA GGTGCTCAGT
    TCACAAGAGA TGCAGGTTGA GAATGAGCTG GAGGACCTGA TAGATGAGTT GCTTGAGAGG
    GATGGCGGAT CTGGGAACAG TACAATTATA GTGAGCAGAA GTGGAGAGGA TGAATCACAA
    GAGGACGTGC TGATGGATGA AGCTCCTTCC AACCTCAGCC AAGCTTCCAC CTTGCAGGCC
    AACCGAGAAG ATTCCATGAA TATCCTGGAC CCTGAGGATG AGGAGGAGCA CACTCAGGAA
    GAGGACAGCA GTGGCAGTAA CGAGGATGAG GATGATAGTC AGGATGAAGA GGAGGAGGAG
    GAGGAAGATG AGGAAGATGA TCAGGAGGAT GATGAAGGTG AAGAGGGAGA TGAAGACGAT
    GACGACGATG GCTCTGAAAT GGAATTGGAT GAGGATTACC CTGATATGAA CGCTTCTCCC
    TTGGTCCGAT TTGAGCGCTT TGACCGGGAG GATGATCTCA TCATTGAGTT TGACAACATG
    TTCTCCAGTG CTACAGACAT CCCCCCATCC CCAGGAAATA TCCCTACCAC CCACCCACTG
    ATGGTACGCC ATGCAGATCA CAGTTCCCTG ACACTGGGCA GTGGCTCCTC TACAACACGT
    CTCACCCAGG GCATTGGGCG CAGTCAAAGG ACCCTGAGAC AGCTGACAGC CAACACAGGC
    CATACCATTC ACGTTCACTA CCCTGGGAAT CGCCAGCCCA ATCCTCCTCT TATACTCCAG
    AGGTTGCTTG GTCCCTCTGC TGCTGCTGAC ATCCTTCAGC TGAGCAGCAG CCTTCCCCTA
    CAAAGCCGAG GCCGAGCCCG CCTCCTAGTG GGCAACGATG ACGTCCACAT CATCGCCCGG
    TCTGATGATG AACTACTGGA TGACTTTTTC CATGATCAGA GCACAGCTAC CAGCCAAGCA
    GGGACCCTAT CCAGTATCCC TACAGCCCTG ACTCGCTGGA CAGAGGAATG CAAAGTTCTT
    GATGCTGAGA GCATGCATGA CTGTGTTTCA GTTGTTAAAG TGCCCATTGT CAATCATCTG
    GAATTCCTGA GGGATGAGGA GCTGGAAGAA AGGAGGGAGA AAAGGAGGAA ACAACTAGCT
    GAGGAAGAAA CAAAGATAAC CGACAAAGGC AAAGAAGATA AGGAGAACAG GGATCAGAGT
    GCACAGTGTG CTGCATCTAA GACAAATGAC TCCACTGAAC AGAACCTCTC AGATGGGACT
    CCTATGCCTG ACAGCTACCC AGCAACCCCA TCTTCAACTG ATGTAGCTAC ATCTGAGCCC
    AGGGAGACCC TTGTCACTCT GCAGCCCTCC CAACAGCAAC CAACACTCCC AACCCCACCA
    GCCTTGGGAG AGATTCCTCA AGAGCTGCAG TCCCCAATTG GAGAAGGTGG GAGCTCTACA
    CAGCTATTGA TGCCTGTAGA GCCAGAGGAA TTGGGTCCCA CAAGGCCAAG TGGAGAAGCA
    GAAACAACTC AGATGGAGCT ATCTCCAGCT CCCACTATAA CCTCTCTTTC CCCAGAGAGA
    GCTGAAGATT CTGATGCTCT GACAGCTGTC AGCAGTCAAC TGGAAGGCTC TCCCATGGAT
    ACTAGCAGCC TGGCTTCCTG TACCCTGGAG GAGGCTGTGG GTGACGCCCC AGCAACTGGC
    AGTTCTGAGC AGCCCACAGC AGGCAGCTCC ACTCCTGGGG ATGCCCCACC AGTTGTGACA
    GAAGTACAAG GCAGGGGTGA TGGGTCAGGG GAACCTACCC AGCCCCCTGA GGACAGCTCT
    CCCCCTGCAT CCTCTGAGAG TTCTTCCACC AGAGATTCCG CGGTGGCCAT TTCTGGAGCA
    GATTCCCGGG GAATCCTGGA AGAGCCATTG CCTTCAACAA GCAGTGAAGA AGAAGATCCT
    CTTGCAGGTA TTAGTCTCCC CGAAGGTGTG GACCCCTCTT TTCTGGCCGC TCTGCCTGAT
    GACATTCGCC GAGAAGTCCT ACAGAACCAG CTGGGCATTC GTCCACCAAC ACGAACTGCC
    CCTTCAACAA ATAACTCAGC TCCTGCAGTG GTGGGGAATC CTGGTGTCAC CGAAGTGAGC
    CCTGAGTTCC TGGCTGCTCT GCCTCCAGCC ATCCAGGAGG AAGTACTGGC ACAGCAGAGA
    GCTGAGCAAC AGCGACGGGA ACTAGCACAA AATGCCAGCT CAGACACCCC CATGGACCCT
    GTGACCTTCA TCCAGACTCT GCCCTCAGAC CTGCGCCGTA GTGTCCTAGA GGACATGGAG
    GACAGTGTGT TGGCCGTGAT GCCACCTGAC ATCGCAGCTG AAGCTCAAGC CCTGAGGCGA
    GAGCAAGAAG CCCGGCAGAG GCAGCTCATG CATGAGCGAC TTTTTGGGCA CAGCAGCACC
    TCTGCACTCT CTGCCATTCT CCGAAGCCCA GCCTTCACCA GTCGCCTGAG TGGCAACCGT
    GGGGTCCAAT ACACTCGCCT TGCTGTGCAA AGAGGTGGCA CCTTCCAGAT GGGGGGTAGC
    AGCAGCCATA ATAGGCCTTC TGGCAGTAAT GTGGACACCC TCCTACGTCT TCGAGGACGG
    CTCCTCCTGG ATCACGAAGC CCTGTCTTGT CTCCTGGTCC TACTTTTTGT GGATGAGCCA
    AAACTCAATA CTAGCCGTCT TCACCGAGTA CTGAGAAATC TCTGCTACCA TGCCCAGACC
    CGCCACTGGG TTATCCGTAG CCTGCTTTCC ATCTTGCAGC GAAGCAGTGA GAGTGAGCTT
    TGCATTGAAA CACCCAAGCT CTCCTCAAGT GAGGAAAAGG GCAAAAAGTC AAGCAAGAGC
    TGTGGGTCAA GCAGCCATGA GAACCGGCCC CTGGACCTGC TACACAAGAT GGAGTCTAAG
    AGTTCCAACC AGCTTTCCTG GCTCTCAGTA TCCATGGATG CAGCCCTAGG CTGCAGGACT
    AATATATTCC AGATCCAGCG TTCAGGGGGT CGCAAACATA CTGAGAAACA TGCGAGTAGT
    GGCTCCACTG TCCATATCCA CCCCCAGGCT GCTCCTGTTG TCTGCAGACA TGTTCTGGAT
    ACACTCATTC AATTGGCCAA GGTGTTTCCC AGTCACTTCA CACAGCAGCG GACCAAAGAA
    ACAAACTGTG AGAGTGATCG GGAGAGGGGC AGTAAGGCCT GCAGCCCATG CTCTTCACAG
    TCCACAAGCA GTGGCATTTG CACAGACTTC TGGGACTTAT TGGTAAAACT GGACAACATG
    AATGTCAGCC GGAAAGGCAA GAACTCCGTG AAGTCAGTGC CAGTGAGCGC TGGTGGTGAG
    GGGGAAACCT CCCCATACAG CCTCGAAGCT TCTCCACTGG GGCAACTCAT GAACATGTTG
    TCACACCCAG TCATCCGCCG GAGCTCTCTC TTAACTGAGA AGCTCCTCAG ACTTCTTTCT
    CTCATCTCAA TTGCTCTCCC AGAAAACAAA GTGTCGGAAG CACAGGCTAA TTCTGGCAGC
    AGTGCTTCCT CCACCACTGT TGCCACTTCA ACCACATCTA CCACCACTAT CAGTGCCACC
    TCCAGCACAT CTACTCTGCC TGCTGCAACC ACCCCTGTCA CTTCTGTTCC AGCCCTGGTT
    GCTGCCACGG CTATATCCAC CATTGCCGTA GCCGCTTCAA CCACAGTGAC TACCCCCACA
    ACTGCTACCA CTACTGTTTC AACTGCTACT ACTACTAAGG CCAGCAAATC TCCAGCTAAA
    GTTGGTGATA TCGGCAGCAG CAGCAGCACA GACTTTAAGA TGGTATCCTC TGGCCTCACT
    GAAAATCAGC TCCAGCTCTC TGTGGAAGTG TTGACATCCC ATTCTTGTTC TGAAGAAGGC
    CTAGAAGATG CAGCAAATGT GTTACTGCAG CTCTCTCGGG GGGACCCTGG GACCCGGGAC
    ACTGTTTTGA AGTTGCTACT GAATGGAGCC CGCCATCTGG GTTATACGCT TTGTAAACAG
    ATAGGAACTC TTCTGGCTGA GCTACGGGAA TACAATCTGG AACAGCAGCG GCGAGCCCAG
    TGTGAAACCC TCTCCCCTGA TGGCATGCCT GAGGAACAGC CACAGACCAC CAAGCTGAAA
    GGCAAAATGC AGAGCAGGTT TGACGTGGCT GAGAATGTGG TAATCGTGGC ATCTCAGAAG
    CGGCCTCTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA
    TCTACCCAGA AGTTCTTCTT GAGGGTACTG CAGGTCATCA TCCAGCTCCG GGATGACACG
    CGCCGTGCTA ACAAGAAGGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA
    GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA
    ATGGTACGTG AGGGTCAGAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCAAGC
    CAGTCAGAGG CATCTGTCCG AAGGGAGGAG TCACCCATGG ATGTGGACCA GCCATCTCCC
    AGTGCTCAAG ATACTCAGTC CATTGGCTCA GATGGAACTC CACAGGGAGA GAAGGAGAAG
    GAGGAGAGGC CACCTGAGTT ACCCCTGCTC AGTGAGCAGC TGAACTTGGA TGAGCTGTGG
    GACATGCTGG GAGAGTGTCT AAAGGAACTG GAGGAATCCC ATGACCAGCA TGCGGTGCTA
    GTGCTACAAC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CAACAGAGCG GGAGAGCAAG
    CCTCCTGTCC GGGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCACCTCCA
    CTCTCCCCTG CCCCTTTAAC CCCAGCCACT CCTTCCTCCC TTGACCCGTT CTTCTCCCGG
    GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTTCTTCGT
    TTTGCAGAGA CTCATCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC CACCCACCTC
    GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT CGATGTCAAG
    CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTGGACGAGG GACTCCGGAA AGAAGACATG
    GCTGTGCACG TTCGTCGTGA TCATGTCTTT GAAGACTCCT ATCGTGAGCT GCACCGGAAA
    TCCCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT
    GCTGGAGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG
    TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGGGTCACAT ACACCATCAA TCCATCTTCT
    CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTTG GACGCATCGT GGCCAAAGCT
    GTGTATGACA ACCGCCTCCT GGAGTGCTAC TTTACTAGGT CCTTCTACAA ACACATTTTG
    GGCAAGTCTG TCAGATATAC AGATATGGAG AGTGAAGACT ACCACTTTTA CCAGGGCCTG
    GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ATCTCACTTT CAGCACTGAG
    GTCCAAGAGT TTGGAGTCTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG
    GTAACAGAGG AAAATAAGAA GGAATATGTA CACCTGGTGT GCCAGATGAG AATGACAGGA
    GCCATCCGCA AACAGCTGGC GGCTTTCTTA GAAGGTTTCT ATGAGATCAT TCCAAAGCGC
    CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTCA TATCAGGACT GCCCACAATC
    GACATTGACG ATTTGAAATC TAACACTGAA TATCACAAGT ACCAGTCCAA CTCTATTCAG
    ATCCAGTGGT TCTGGAGAGC ACTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC
    CAGTTTGTCA CAGGTACTTC CAAGGTGCCC CTGCAAGGTT TTGCTGCCCT TGAAGGCATG
    AATGGCATTC AGAAGTTTCA AATCCATCGA GATGACAGGT CCACAGACCG CCTGCCTTCA
    GCTCACACAT GTTTTAATCA GCTGGACCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC
    CACATGCTAC TATTGGCCAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_010821989.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821989.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820291.1
    CDS734..14008
    Translation

    Target ORF information:

    RefSeq Version XM_010821989.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821989.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_010821994.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
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    2641
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    4141
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    4381
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    4681
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    5281
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    5521
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    5881
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    9061
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    9181
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    9301
    9361
    9421
    9481
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    9661
    9721
    9781
    9841
    9901
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    10201
    10261
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    10381
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    10501
    10561
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    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820296.1
    CDS547..13821
    Translation

    Target ORF information:

    RefSeq Version XM_010821994.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821994.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_015461711.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
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    2281
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    2641
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    9781
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    10501
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    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317197.1
    CDS509..13783
    Translation

    Target ORF information:

    RefSeq Version XM_015461711.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461711.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_024987889.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
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    12061
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    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843657.1
    CDS724..13998
    Translation

    Target ORF information:

    RefSeq Version XM_024987889.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987889.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_010821990.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821990.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
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    2641
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    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820292.1
    CDS370..13644
    Translation

    Target ORF information:

    RefSeq Version XM_010821990.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821990.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_015461710.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
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    3181
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    3421
    3481
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    4021
    4081
    4141
    4201
    4261
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    4381
    4441
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    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
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    5821
    5881
    5941
    6001
    6061
    6121
    6181
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    6301
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    6421
    6481
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    7141
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    9061
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    9301
    9361
    9421
    9481
    9541
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    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
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    11401
    11461
    11521
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    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317196.1
    CDS606..13880
    Translation

    Target ORF information:

    RefSeq Version XM_015461710.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461710.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_015461712.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
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    7621
    7681
    7741
    7801
    7861
    7921
    7981
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    8401
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    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317198.1
    CDS223..13497
    Translation

    Target ORF information:

    RefSeq Version XM_015461712.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461712.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_010821990.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010821990.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820292.1
    CDS370..13644
    Translation

    Target ORF information:

    RefSeq Version XM_010821990.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821990.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_010821994.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010821994.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820296.1
    CDS538..13812
    Translation

    Target ORF information:

    RefSeq Version XM_010821994.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821994.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_015461710.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461710.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317196.1
    CDS597..13871
    Translation

    Target ORF information:

    RefSeq Version XM_015461710.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461710.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_024987890.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843658.1
    CDS178..13452
    Translation

    Target ORF information:

    RefSeq Version XM_024987890.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987890.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_010821992.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    3601
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    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
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    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820294.1
    CDS450..13724
    Translation

    Target ORF information:

    RefSeq Version XM_010821992.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821992.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_015461711.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
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    601
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    721
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    841
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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
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    9301
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    9421
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    9721
    9781
    9841
    9901
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    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317197.1
    CDS500..13774
    Translation

    Target ORF information:

    RefSeq Version XM_015461711.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461711.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_024987891.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
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    2281
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    2641
    2701
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    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843659.1
    CDS487..13761
    Translation

    Target ORF information:

    RefSeq Version XM_024987891.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987891.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_010821995.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821995.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
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    2941
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    4081
    4141
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    4261
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    4381
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    4681
    4741
    4801
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    4981
    5041
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    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
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    5821
    5881
    5941
    6001
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    6181
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    6301
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    7141
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    9061
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    9301
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    9421
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    9721
    9781
    9841
    9901
    9961
    10021
    10081
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    10261
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    10381
    10441
    10501
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    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820297.1
    CDS851..14125
    Translation

    Target ORF information:

    RefSeq Version XM_010821995.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821995.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_010821992.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010821992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820294.1
    CDS441..13715
    Translation

    Target ORF information:

    RefSeq Version XM_010821992.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821992.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48119
    Clone ID Related Accession (Same CDS sequence) XM_010821989.2 , XM_010821994.2 , XM_015461711.1 , XM_024987889.1 , XM_010821990.2 , XM_015461710.1 , XM_015461712.1 , XM_010821990.3 , XM_010821994.3 , XM_015461710.2 , XM_024987890.1 , XM_010821992.2 , XM_015461711.2 , XM_024987891.1 , XM_010821995.2 , XM_010821992.3 , XM_015461712.2
    Accession Version XM_015461712.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461712.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317198.1
    CDS476..13750
    Translation

    Target ORF information:

    RefSeq Version XM_015461712.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461712.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48120
    Clone ID Related Accession (Same CDS sequence) XM_024987893.1 , XM_010821996.2
    Accession Version XM_024987893.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    AAGAGAGCTG AAGATTCTGA TGCTCTGACA GCTGTCAGCA GTCAACTGGA AGGCTCTCCC
    ATGGATACTA GCAGCCTGGC TTCCTGTACC CTGGAGGAGG CTGTGGGTGA CGCCCCAGCA
    ACTGGCAGTT CTGAGCAGCC CACAGCAGGC AGCTCCACTC CTGGGGATGC CCCACCAGTT
    GTGACAGAAG TACAAGGCAG GGGTGATGGG TCAGGGGAAC CTACCCAGCC CCCTGAGGAC
    AGCTCTCCCC CTGCATCCTC TGAGAGTTCT TCCACCAGAG ATTCCGCGGT GGCCATTTCT
    GGAGCAGATT CCCGGGGAAT CCTGGAAGAG CCATTGCCTT CAACAAGCAG TGAAGAAGAA
    GATCCTCTTG CAGGTATTAG TCTCCCCGAA GGTGTGGACC CCTCTTTTCT GGCCGCTCTG
    CCTGATGACA TTCGCCGAGA AGTCCTACAG AACCAGCTGG GCATTCGTCC ACCAACACGA
    ACTGCCCCTT CAACAAATAA CTCAGCTCCT GCAGTGGTGG GGAATCCTGG TGTCACCGAA
    GTGAGCCCTG AGTTCCTGGC TGCTCTGCCT CCAGCCATCC AGGAGGAAGT ACTGGCACAG
    CAGAGAGCTG AGCAACAGCG ACGGGAACTA GCACAAAATG CCAGCTCAGA CACCCCCATG
    GACCCTGTGA CCTTCATCCA GACTCTGCCC TCAGACCTGC GCCGTAGTGT CCTAGAGGAC
    ATGGAGGACA GTGTGTTGGC CGTGATGCCA CCTGACATCG CAGCTGAAGC TCAAGCCCTG
    AGGCGAGAGC AAGAAGCCCG GCAGAGGCAG CTCATGCATG AGCGACTTTT TGGGCACAGC
    AGCACCTCTG CACTCTCTGC CATTCTCCGA AGCCCAGCCT TCACCAGTCG CCTGAGTGGC
    AACCGTGGGG TCCAATACAC TCGCCTTGCT GTGCAAAGAG GTGGCACCTT CCAGATGGGG
    GGTAGCAGCA GCCATAATAG GCCTTCTGGC AGTAATGTGG ACACCCTCCT ACGTCTTCGA
    GGACGGCTCC TCCTGGATCA CGAAGCCCTG TCTTGTCTCC TGGTCCTACT TTTTGTGGAT
    GAGCCAAAAC TCAATACTAG CCGTCTTCAC CGAGTACTGA GAAATCTCTG CTACCATGCC
    CAGACCCGCC ACTGGGTTAT CCGTAGCCTG CTTTCCATCT TGCAGCGAAG CAGTGAGAGT
    GAGCTTTGCA TTGAAACACC CAAGCTCTCC TCAAGTGAGG AAAAGGGCAA AAAGTCAAGC
    AAGAGCTGTG GGTCAAGCAG CCATGAGAAC CGGCCCCTGG ACCTGCTACA CAAGATGGAG
    TCTAAGAGTT CCAACCAGCT TTCCTGGCTC TCAGTATCCA TGGATGCAGC CCTAGGCTGC
    AGGACTAATA TATTCCAGAT CCAGCGTTCA GGGGGTCGCA AACATACTGA GAAACATGCG
    AGTAGTGGCT CCACTGTCCA TATCCACCCC CAGGCTGCTC CTGTTGTCTG CAGACATGTT
    CTGGATACAC TCATTCAATT GGCCAAGGTG TTTCCCAGTC ACTTCACACA GCAGCGGACC
    AAAGAAACAA ACTGTGAGAG TGATCGGGAG AGGGGCAGTA AGGCCTGCAG CCCATGCTCT
    TCACAGTCCA CAAGCAGTGG CATTTGCACA GACTTCTGGG ACTTATTGGT AAAACTGGAC
    AACATGAATG TCAGCCGGAA AGGCAAGAAC TCCGTGAAGT CAGTGCCAGT GAGCGCTGGT
    GGTGAGGGGG AAACCTCCCC ATACAGCCTC GAAGCTTCTC CACTGGGGCA ACTCATGAAC
    ATGTTGTCAC ACCCAGTCAT CCGCCGGAGC TCTCTCTTAA CTGAGAAGCT CCTCAGACTT
    CTTTCTCTCA TCTCAATTGC TCTCCCAGAA AACAAAGTGT CGGAAGCACA GGCTAATTCT
    GGCAGCAGTG CTTCCTCCAC CACTGTTGCC ACTTCAACCA CATCTACCAC CACTATCAGT
    GCCACCTCCA GCACATCTAC TCTGCCTGCT GCAACCACCC CTGTCACTTC TGTTCCAGCC
    CTGGTTGCTG CCACGGCTAT ATCCACCATT GCCGTAGCCG CTTCAACCAC AGTGACTACC
    CCCACAACTG CTACCACTAC TGTTTCAACT GCTACTACTA CTAAGGCCAG CAAATCTCCA
    GCTAAAGTTG GTGATATCGG CAGCAGCAGC AGCACAGACT TTAAGATGGT ATCCTCTGGC
    CTCACTGAAA ATCAGCTCCA GCTCTCTGTG GAAGTGTTGA CATCCCATTC TTGTTCTGAA
    GAAGGCCTAG AAGATGCAGC AAATGTGTTA CTGCAGCTCT CTCGGGGGGA CCCTGGGACC
    CGGGACACTG TTTTGAAGTT GCTACTGAAT GGAGCCCGCC ATCTGGGTTA TACGCTTTGT
    AAACAGATAG GAACTCTTCT GGCTGAGCTA CGGGAATACA ATCTGGAACA GCAGCGGCGA
    GCCCAGTGTG AAACCCTCTC CCCTGATGGC ATGCCTGAGG AACAGCCACA GACCACCAAG
    CTGAAAGGCA AAATGCAGAG CAGGTTTGAC GTGGCTGAGA ATGTGGTAAT CGTGGCATCT
    CAGAAGCGGC CTCTGGGTGG CCGGGAGCTC CAGCTGCCTT CTATGTCCAT GTTGACATCC
    AAGACATCTA CCCAGAAGTT CTTCTTGAGG GTACTGCAGG TCATCATCCA GCTCCGGGAT
    GACACGCGCC GTGCTAACAA GAAGGCCAAG CAGACAGGCA GGCTAGGTTC CTCCGGTTTA
    GGCTCAGCTA GCAGCATCCA GGCAGCTGTT CGGCAGCTGG AGGCTGAGGC TGATGCCATT
    ATACAAATGG TACGTGAGGG TCAGAGGGCG CGGAGACAGC AACAAGCAGC AACGTCGGAG
    TCAAGCCAGT CAGAGGCATC TGTCCGAAGG GAGGAGTCAC CCATGGATGT GGACCAGCCA
    TCTCCCAGTG CTCAAGATAC TCAGTCCATT GGCTCAGATG GAACTCCACA GGGAGAGAAG
    GAGAAGGAGG AGAGGCCACC TGAGTTACCC CTGCTCAGTG AGCAGCTGAA CTTGGATGAG
    CTGTGGGACA TGCTGGGAGA GTGTCTAAAG GAACTGGAGG AATCCCATGA CCAGCATGCG
    GTGCTAGTGC TACAACCTGC TGTCGAGGCC TTCTTTCTGG TCCATGCAAC AGAGCGGGAG
    AGCAAGCCTC CTGTCCGGGA CACCCGTGAG AGCCAGCTGG CACACATCAA GGACGAGCCA
    CCTCCACTCT CCCCTGCCCC TTTAACCCCA GCCACTCCTT CCTCCCTTGA CCCGTTCTTC
    TCCCGGGAGC CCTCATCTAT GCACATCTCC TCAAGCCTGC CCCCTGACAC ACAGAAGTTT
    CTTCGTTTTG CAGAGACTCA TCGCACTGTG TTAAACCAGA TCCTACGGCA GTCCACCACC
    CACCTCGCTG ATGGGCCTTT TGCTGTCCTG GTAGACTACA TTCGTGTCCT CGACTTCGAT
    GTCAAGCGCA AATATTTCCG CCAAGAGCTG GAGCGTTTGG ACGAGGGACT CCGGAAAGAA
    GACATGGCTG TGCACGTTCG TCGTGATCAT GTCTTTGAAG ACTCCTATCG TGAGCTGCAC
    CGGAAATCCC CTGAAGAAAT GAAGAATCGA TTGTATATAG TATTTGAAGG AGAAGAAGGG
    CAGGATGCTG GAGGGCTCCT GCGGGAGTGG TATATGATCA TCTCTCGAGA GATGTTTAAC
    CCTATGTATG CCTTGTTCCG TACCTCACCT GGTGATCGGG TCACATACAC CATCAATCCA
    TCTTCTCACT GCAACCCCAA CCACCTCAGC TACTTCAAGT TTGTTGGACG CATCGTGGCC
    AAAGCTGTGT ATGACAACCG CCTCCTGGAG TGCTACTTTA CTAGGTCCTT CTACAAACAC
    ATTTTGGGCA AGTCTGTCAG ATATACAGAT ATGGAGAGTG AAGACTACCA CTTTTACCAG
    GGCCTGGTTT ATCTGCTGGA AAATGATGTC TCCACACTAG GCTATGATCT CACTTTCAGC
    ACTGAGGTCC AAGAGTTTGG AGTCTGTGAA GTTCGTGACC TCAAACCCAA TGGGGCCAAC
    ATCTTGGTAA CAGAGGAAAA TAAGAAGGAA TATGTACACC TGGTGTGCCA GATGAGAATG
    ACAGGAGCCA TCCGCAAACA GCTGGCGGCT TTCTTAGAAG GTTTCTATGA GATCATTCCA
    AAGCGCCTCA TTTCCATCTT CACTGAGCAG GAGTTAGAGC TGCTCATATC AGGACTGCCC
    ACAATCGACA TTGACGATTT GAAATCTAAC ACTGAATATC ACAAGTACCA GTCCAACTCT
    ATTCAGATCC AGTGGTTCTG GAGAGCACTG CGTTCTTTCG ATCAAGCTGA CCGTGCCAAG
    TTCCTCCAGT TTGTCACAGG TACTTCCAAG GTGCCCCTGC AAGGTTTTGC TGCCCTTGAA
    GGCATGAATG GCATTCAGAA GTTTCAAATC CATCGAGATG ACAGGTCCAC AGACCGCCTG
    CCTTCAGCTC ACACATGTTT TAATCAGCTG GACCTGCCTG CCTATGAGAG CTTTGAGAAG
    CTCCGCCACA TGCTACTATT GGCCATCCAG GAGTGCTCTG AAGGCTTTGG GCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843661.1
    CDS442..13701
    Translation

    Target ORF information:

    RefSeq Version XM_024987893.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987893.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    AAGAGAGCTG AAGATTCTGA TGCTCTGACA GCTGTCAGCA GTCAACTGGA AGGCTCTCCC
    ATGGATACTA GCAGCCTGGC TTCCTGTACC CTGGAGGAGG CTGTGGGTGA CGCCCCAGCA
    ACTGGCAGTT CTGAGCAGCC CACAGCAGGC AGCTCCACTC CTGGGGATGC CCCACCAGTT
    GTGACAGAAG TACAAGGCAG GGGTGATGGG TCAGGGGAAC CTACCCAGCC CCCTGAGGAC
    AGCTCTCCCC CTGCATCCTC TGAGAGTTCT TCCACCAGAG ATTCCGCGGT GGCCATTTCT
    GGAGCAGATT CCCGGGGAAT CCTGGAAGAG CCATTGCCTT CAACAAGCAG TGAAGAAGAA
    GATCCTCTTG CAGGTATTAG TCTCCCCGAA GGTGTGGACC CCTCTTTTCT GGCCGCTCTG
    CCTGATGACA TTCGCCGAGA AGTCCTACAG AACCAGCTGG GCATTCGTCC ACCAACACGA
    ACTGCCCCTT CAACAAATAA CTCAGCTCCT GCAGTGGTGG GGAATCCTGG TGTCACCGAA
    GTGAGCCCTG AGTTCCTGGC TGCTCTGCCT CCAGCCATCC AGGAGGAAGT ACTGGCACAG
    CAGAGAGCTG AGCAACAGCG ACGGGAACTA GCACAAAATG CCAGCTCAGA CACCCCCATG
    GACCCTGTGA CCTTCATCCA GACTCTGCCC TCAGACCTGC GCCGTAGTGT CCTAGAGGAC
    ATGGAGGACA GTGTGTTGGC CGTGATGCCA CCTGACATCG CAGCTGAAGC TCAAGCCCTG
    AGGCGAGAGC AAGAAGCCCG GCAGAGGCAG CTCATGCATG AGCGACTTTT TGGGCACAGC
    AGCACCTCTG CACTCTCTGC CATTCTCCGA AGCCCAGCCT TCACCAGTCG CCTGAGTGGC
    AACCGTGGGG TCCAATACAC TCGCCTTGCT GTGCAAAGAG GTGGCACCTT CCAGATGGGG
    GGTAGCAGCA GCCATAATAG GCCTTCTGGC AGTAATGTGG ACACCCTCCT ACGTCTTCGA
    GGACGGCTCC TCCTGGATCA CGAAGCCCTG TCTTGTCTCC TGGTCCTACT TTTTGTGGAT
    GAGCCAAAAC TCAATACTAG CCGTCTTCAC CGAGTACTGA GAAATCTCTG CTACCATGCC
    CAGACCCGCC ACTGGGTTAT CCGTAGCCTG CTTTCCATCT TGCAGCGAAG CAGTGAGAGT
    GAGCTTTGCA TTGAAACACC CAAGCTCTCC TCAAGTGAGG AAAAGGGCAA AAAGTCAAGC
    AAGAGCTGTG GGTCAAGCAG CCATGAGAAC CGGCCCCTGG ACCTGCTACA CAAGATGGAG
    TCTAAGAGTT CCAACCAGCT TTCCTGGCTC TCAGTATCCA TGGATGCAGC CCTAGGCTGC
    AGGACTAATA TATTCCAGAT CCAGCGTTCA GGGGGTCGCA AACATACTGA GAAACATGCG
    AGTAGTGGCT CCACTGTCCA TATCCACCCC CAGGCTGCTC CTGTTGTCTG CAGACATGTT
    CTGGATACAC TCATTCAATT GGCCAAGGTG TTTCCCAGTC ACTTCACACA GCAGCGGACC
    AAAGAAACAA ACTGTGAGAG TGATCGGGAG AGGGGCAGTA AGGCCTGCAG CCCATGCTCT
    TCACAGTCCA CAAGCAGTGG CATTTGCACA GACTTCTGGG ACTTATTGGT AAAACTGGAC
    AACATGAATG TCAGCCGGAA AGGCAAGAAC TCCGTGAAGT CAGTGCCAGT GAGCGCTGGT
    GGTGAGGGGG AAACCTCCCC ATACAGCCTC GAAGCTTCTC CACTGGGGCA ACTCATGAAC
    ATGTTGTCAC ACCCAGTCAT CCGCCGGAGC TCTCTCTTAA CTGAGAAGCT CCTCAGACTT
    CTTTCTCTCA TCTCAATTGC TCTCCCAGAA AACAAAGTGT CGGAAGCACA GGCTAATTCT
    GGCAGCAGTG CTTCCTCCAC CACTGTTGCC ACTTCAACCA CATCTACCAC CACTATCAGT
    GCCACCTCCA GCACATCTAC TCTGCCTGCT GCAACCACCC CTGTCACTTC TGTTCCAGCC
    CTGGTTGCTG CCACGGCTAT ATCCACCATT GCCGTAGCCG CTTCAACCAC AGTGACTACC
    CCCACAACTG CTACCACTAC TGTTTCAACT GCTACTACTA CTAAGGCCAG CAAATCTCCA
    GCTAAAGTTG GTGATATCGG CAGCAGCAGC AGCACAGACT TTAAGATGGT ATCCTCTGGC
    CTCACTGAAA ATCAGCTCCA GCTCTCTGTG GAAGTGTTGA CATCCCATTC TTGTTCTGAA
    GAAGGCCTAG AAGATGCAGC AAATGTGTTA CTGCAGCTCT CTCGGGGGGA CCCTGGGACC
    CGGGACACTG TTTTGAAGTT GCTACTGAAT GGAGCCCGCC ATCTGGGTTA TACGCTTTGT
    AAACAGATAG GAACTCTTCT GGCTGAGCTA CGGGAATACA ATCTGGAACA GCAGCGGCGA
    GCCCAGTGTG AAACCCTCTC CCCTGATGGC ATGCCTGAGG AACAGCCACA GACCACCAAG
    CTGAAAGGCA AAATGCAGAG CAGGTTTGAC GTGGCTGAGA ATGTGGTAAT CGTGGCATCT
    CAGAAGCGGC CTCTGGGTGG CCGGGAGCTC CAGCTGCCTT CTATGTCCAT GTTGACATCC
    AAGACATCTA CCCAGAAGTT CTTCTTGAGG GTACTGCAGG TCATCATCCA GCTCCGGGAT
    GACACGCGCC GTGCTAACAA GAAGGCCAAG CAGACAGGCA GGCTAGGTTC CTCCGGTTTA
    GGCTCAGCTA GCAGCATCCA GGCAGCTGTT CGGCAGCTGG AGGCTGAGGC TGATGCCATT
    ATACAAATGG TACGTGAGGG TCAGAGGGCG CGGAGACAGC AACAAGCAGC AACGTCGGAG
    TCAAGCCAGT CAGAGGCATC TGTCCGAAGG GAGGAGTCAC CCATGGATGT GGACCAGCCA
    TCTCCCAGTG CTCAAGATAC TCAGTCCATT GGCTCAGATG GAACTCCACA GGGAGAGAAG
    GAGAAGGAGG AGAGGCCACC TGAGTTACCC CTGCTCAGTG AGCAGCTGAA CTTGGATGAG
    CTGTGGGACA TGCTGGGAGA GTGTCTAAAG GAACTGGAGG AATCCCATGA CCAGCATGCG
    GTGCTAGTGC TACAACCTGC TGTCGAGGCC TTCTTTCTGG TCCATGCAAC AGAGCGGGAG
    AGCAAGCCTC CTGTCCGGGA CACCCGTGAG AGCCAGCTGG CACACATCAA GGACGAGCCA
    CCTCCACTCT CCCCTGCCCC TTTAACCCCA GCCACTCCTT CCTCCCTTGA CCCGTTCTTC
    TCCCGGGAGC CCTCATCTAT GCACATCTCC TCAAGCCTGC CCCCTGACAC ACAGAAGTTT
    CTTCGTTTTG CAGAGACTCA TCGCACTGTG TTAAACCAGA TCCTACGGCA GTCCACCACC
    CACCTCGCTG ATGGGCCTTT TGCTGTCCTG GTAGACTACA TTCGTGTCCT CGACTTCGAT
    GTCAAGCGCA AATATTTCCG CCAAGAGCTG GAGCGTTTGG ACGAGGGACT CCGGAAAGAA
    GACATGGCTG TGCACGTTCG TCGTGATCAT GTCTTTGAAG ACTCCTATCG TGAGCTGCAC
    CGGAAATCCC CTGAAGAAAT GAAGAATCGA TTGTATATAG TATTTGAAGG AGAAGAAGGG
    CAGGATGCTG GAGGGCTCCT GCGGGAGTGG TATATGATCA TCTCTCGAGA GATGTTTAAC
    CCTATGTATG CCTTGTTCCG TACCTCACCT GGTGATCGGG TCACATACAC CATCAATCCA
    TCTTCTCACT GCAACCCCAA CCACCTCAGC TACTTCAAGT TTGTTGGACG CATCGTGGCC
    AAAGCTGTGT ATGACAACCG CCTCCTGGAG TGCTACTTTA CTAGGTCCTT CTACAAACAC
    ATTTTGGGCA AGTCTGTCAG ATATACAGAT ATGGAGAGTG AAGACTACCA CTTTTACCAG
    GGCCTGGTTT ATCTGCTGGA AAATGATGTC TCCACACTAG GCTATGATCT CACTTTCAGC
    ACTGAGGTCC AAGAGTTTGG AGTCTGTGAA GTTCGTGACC TCAAACCCAA TGGGGCCAAC
    ATCTTGGTAA CAGAGGAAAA TAAGAAGGAA TATGTACACC TGGTGTGCCA GATGAGAATG
    ACAGGAGCCA TCCGCAAACA GCTGGCGGCT TTCTTAGAAG GTTTCTATGA GATCATTCCA
    AAGCGCCTCA TTTCCATCTT CACTGAGCAG GAGTTAGAGC TGCTCATATC AGGACTGCCC
    ACAATCGACA TTGACGATTT GAAATCTAAC ACTGAATATC ACAAGTACCA GTCCAACTCT
    ATTCAGATCC AGTGGTTCTG GAGAGCACTG CGTTCTTTCG ATCAAGCTGA CCGTGCCAAG
    TTCCTCCAGT TTGTCACAGG TACTTCCAAG GTGCCCCTGC AAGGTTTTGC TGCCCTTGAA
    GGCATGAATG GCATTCAGAA GTTTCAAATC CATCGAGATG ACAGGTCCAC AGACCGCCTG
    CCTTCAGCTC ACACATGTTT TAATCAGCTG GACCTGCCTG CCTATGAGAG CTTTGAGAAG
    CTCCGCCACA TGCTACTATT GGCCATCCAG GAGTGCTCTG AAGGCTTTGG GCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48120
    Clone ID Related Accession (Same CDS sequence) XM_024987893.1 , XM_010821996.2
    Accession Version XM_010821996.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821996.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    AAGAGAGCTG AAGATTCTGA TGCTCTGACA GCTGTCAGCA GTCAACTGGA AGGCTCTCCC
    ATGGATACTA GCAGCCTGGC TTCCTGTACC CTGGAGGAGG CTGTGGGTGA CGCCCCAGCA
    ACTGGCAGTT CTGAGCAGCC CACAGCAGGC AGCTCCACTC CTGGGGATGC CCCACCAGTT
    GTGACAGAAG TACAAGGCAG GGGTGATGGG TCAGGGGAAC CTACCCAGCC CCCTGAGGAC
    AGCTCTCCCC CTGCATCCTC TGAGAGTTCT TCCACCAGAG ATTCCGCGGT GGCCATTTCT
    GGAGCAGATT CCCGGGGAAT CCTGGAAGAG CCATTGCCTT CAACAAGCAG TGAAGAAGAA
    GATCCTCTTG CAGGTATTAG TCTCCCCGAA GGTGTGGACC CCTCTTTTCT GGCCGCTCTG
    CCTGATGACA TTCGCCGAGA AGTCCTACAG AACCAGCTGG GCATTCGTCC ACCAACACGA
    ACTGCCCCTT CAACAAATAA CTCAGCTCCT GCAGTGGTGG GGAATCCTGG TGTCACCGAA
    GTGAGCCCTG AGTTCCTGGC TGCTCTGCCT CCAGCCATCC AGGAGGAAGT ACTGGCACAG
    CAGAGAGCTG AGCAACAGCG ACGGGAACTA GCACAAAATG CCAGCTCAGA CACCCCCATG
    GACCCTGTGA CCTTCATCCA GACTCTGCCC TCAGACCTGC GCCGTAGTGT CCTAGAGGAC
    ATGGAGGACA GTGTGTTGGC CGTGATGCCA CCTGACATCG CAGCTGAAGC TCAAGCCCTG
    AGGCGAGAGC AAGAAGCCCG GCAGAGGCAG CTCATGCATG AGCGACTTTT TGGGCACAGC
    AGCACCTCTG CACTCTCTGC CATTCTCCGA AGCCCAGCCT TCACCAGTCG CCTGAGTGGC
    AACCGTGGGG TCCAATACAC TCGCCTTGCT GTGCAAAGAG GTGGCACCTT CCAGATGGGG
    GGTAGCAGCA GCCATAATAG GCCTTCTGGC AGTAATGTGG ACACCCTCCT ACGTCTTCGA
    GGACGGCTCC TCCTGGATCA CGAAGCCCTG TCTTGTCTCC TGGTCCTACT TTTTGTGGAT
    GAGCCAAAAC TCAATACTAG CCGTCTTCAC CGAGTACTGA GAAATCTCTG CTACCATGCC
    CAGACCCGCC ACTGGGTTAT CCGTAGCCTG CTTTCCATCT TGCAGCGAAG CAGTGAGAGT
    GAGCTTTGCA TTGAAACACC CAAGCTCTCC TCAAGTGAGG AAAAGGGCAA AAAGTCAAGC
    AAGAGCTGTG GGTCAAGCAG CCATGAGAAC CGGCCCCTGG ACCTGCTACA CAAGATGGAG
    TCTAAGAGTT CCAACCAGCT TTCCTGGCTC TCAGTATCCA TGGATGCAGC CCTAGGCTGC
    AGGACTAATA TATTCCAGAT CCAGCGTTCA GGGGGTCGCA AACATACTGA GAAACATGCG
    AGTAGTGGCT CCACTGTCCA TATCCACCCC CAGGCTGCTC CTGTTGTCTG CAGACATGTT
    CTGGATACAC TCATTCAATT GGCCAAGGTG TTTCCCAGTC ACTTCACACA GCAGCGGACC
    AAAGAAACAA ACTGTGAGAG TGATCGGGAG AGGGGCAGTA AGGCCTGCAG CCCATGCTCT
    TCACAGTCCA CAAGCAGTGG CATTTGCACA GACTTCTGGG ACTTATTGGT AAAACTGGAC
    AACATGAATG TCAGCCGGAA AGGCAAGAAC TCCGTGAAGT CAGTGCCAGT GAGCGCTGGT
    GGTGAGGGGG AAACCTCCCC ATACAGCCTC GAAGCTTCTC CACTGGGGCA ACTCATGAAC
    ATGTTGTCAC ACCCAGTCAT CCGCCGGAGC TCTCTCTTAA CTGAGAAGCT CCTCAGACTT
    CTTTCTCTCA TCTCAATTGC TCTCCCAGAA AACAAAGTGT CGGAAGCACA GGCTAATTCT
    GGCAGCAGTG CTTCCTCCAC CACTGTTGCC ACTTCAACCA CATCTACCAC CACTATCAGT
    GCCACCTCCA GCACATCTAC TCTGCCTGCT GCAACCACCC CTGTCACTTC TGTTCCAGCC
    CTGGTTGCTG CCACGGCTAT ATCCACCATT GCCGTAGCCG CTTCAACCAC AGTGACTACC
    CCCACAACTG CTACCACTAC TGTTTCAACT GCTACTACTA CTAAGGCCAG CAAATCTCCA
    GCTAAAGTTG GTGATATCGG CAGCAGCAGC AGCACAGACT TTAAGATGGT ATCCTCTGGC
    CTCACTGAAA ATCAGCTCCA GCTCTCTGTG GAAGTGTTGA CATCCCATTC TTGTTCTGAA
    GAAGGCCTAG AAGATGCAGC AAATGTGTTA CTGCAGCTCT CTCGGGGGGA CCCTGGGACC
    CGGGACACTG TTTTGAAGTT GCTACTGAAT GGAGCCCGCC ATCTGGGTTA TACGCTTTGT
    AAACAGATAG GAACTCTTCT GGCTGAGCTA CGGGAATACA ATCTGGAACA GCAGCGGCGA
    GCCCAGTGTG AAACCCTCTC CCCTGATGGC ATGCCTGAGG AACAGCCACA GACCACCAAG
    CTGAAAGGCA AAATGCAGAG CAGGTTTGAC GTGGCTGAGA ATGTGGTAAT CGTGGCATCT
    CAGAAGCGGC CTCTGGGTGG CCGGGAGCTC CAGCTGCCTT CTATGTCCAT GTTGACATCC
    AAGACATCTA CCCAGAAGTT CTTCTTGAGG GTACTGCAGG TCATCATCCA GCTCCGGGAT
    GACACGCGCC GTGCTAACAA GAAGGCCAAG CAGACAGGCA GGCTAGGTTC CTCCGGTTTA
    GGCTCAGCTA GCAGCATCCA GGCAGCTGTT CGGCAGCTGG AGGCTGAGGC TGATGCCATT
    ATACAAATGG TACGTGAGGG TCAGAGGGCG CGGAGACAGC AACAAGCAGC AACGTCGGAG
    TCAAGCCAGT CAGAGGCATC TGTCCGAAGG GAGGAGTCAC CCATGGATGT GGACCAGCCA
    TCTCCCAGTG CTCAAGATAC TCAGTCCATT GGCTCAGATG GAACTCCACA GGGAGAGAAG
    GAGAAGGAGG AGAGGCCACC TGAGTTACCC CTGCTCAGTG AGCAGCTGAA CTTGGATGAG
    CTGTGGGACA TGCTGGGAGA GTGTCTAAAG GAACTGGAGG AATCCCATGA CCAGCATGCG
    GTGCTAGTGC TACAACCTGC TGTCGAGGCC TTCTTTCTGG TCCATGCAAC AGAGCGGGAG
    AGCAAGCCTC CTGTCCGGGA CACCCGTGAG AGCCAGCTGG CACACATCAA GGACGAGCCA
    CCTCCACTCT CCCCTGCCCC TTTAACCCCA GCCACTCCTT CCTCCCTTGA CCCGTTCTTC
    TCCCGGGAGC CCTCATCTAT GCACATCTCC TCAAGCCTGC CCCCTGACAC ACAGAAGTTT
    CTTCGTTTTG CAGAGACTCA TCGCACTGTG TTAAACCAGA TCCTACGGCA GTCCACCACC
    CACCTCGCTG ATGGGCCTTT TGCTGTCCTG GTAGACTACA TTCGTGTCCT CGACTTCGAT
    GTCAAGCGCA AATATTTCCG CCAAGAGCTG GAGCGTTTGG ACGAGGGACT CCGGAAAGAA
    GACATGGCTG TGCACGTTCG TCGTGATCAT GTCTTTGAAG ACTCCTATCG TGAGCTGCAC
    CGGAAATCCC CTGAAGAAAT GAAGAATCGA TTGTATATAG TATTTGAAGG AGAAGAAGGG
    CAGGATGCTG GAGGGCTCCT GCGGGAGTGG TATATGATCA TCTCTCGAGA GATGTTTAAC
    CCTATGTATG CCTTGTTCCG TACCTCACCT GGTGATCGGG TCACATACAC CATCAATCCA
    TCTTCTCACT GCAACCCCAA CCACCTCAGC TACTTCAAGT TTGTTGGACG CATCGTGGCC
    AAAGCTGTGT ATGACAACCG CCTCCTGGAG TGCTACTTTA CTAGGTCCTT CTACAAACAC
    ATTTTGGGCA AGTCTGTCAG ATATACAGAT ATGGAGAGTG AAGACTACCA CTTTTACCAG
    GGCCTGGTTT ATCTGCTGGA AAATGATGTC TCCACACTAG GCTATGATCT CACTTTCAGC
    ACTGAGGTCC AAGAGTTTGG AGTCTGTGAA GTTCGTGACC TCAAACCCAA TGGGGCCAAC
    ATCTTGGTAA CAGAGGAAAA TAAGAAGGAA TATGTACACC TGGTGTGCCA GATGAGAATG
    ACAGGAGCCA TCCGCAAACA GCTGGCGGCT TTCTTAGAAG GTTTCTATGA GATCATTCCA
    AAGCGCCTCA TTTCCATCTT CACTGAGCAG GAGTTAGAGC TGCTCATATC AGGACTGCCC
    ACAATCGACA TTGACGATTT GAAATCTAAC ACTGAATATC ACAAGTACCA GTCCAACTCT
    ATTCAGATCC AGTGGTTCTG GAGAGCACTG CGTTCTTTCG ATCAAGCTGA CCGTGCCAAG
    TTCCTCCAGT TTGTCACAGG TACTTCCAAG GTGCCCCTGC AAGGTTTTGC TGCCCTTGAA
    GGCATGAATG GCATTCAGAA GTTTCAAATC CATCGAGATG ACAGGTCCAC AGACCGCCTG
    CCTTCAGCTC ACACATGTTT TAATCAGCTG GACCTGCCTG CCTATGAGAG CTTTGAGAAG
    CTCCGCCACA TGCTACTATT GGCCATCCAG GAGTGCTCTG AAGGCTTTGG GCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820298.1
    CDS734..13993
    Translation

    Target ORF information:

    RefSeq Version XM_010821996.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821996.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    AAGAGAGCTG AAGATTCTGA TGCTCTGACA GCTGTCAGCA GTCAACTGGA AGGCTCTCCC
    ATGGATACTA GCAGCCTGGC TTCCTGTACC CTGGAGGAGG CTGTGGGTGA CGCCCCAGCA
    ACTGGCAGTT CTGAGCAGCC CACAGCAGGC AGCTCCACTC CTGGGGATGC CCCACCAGTT
    GTGACAGAAG TACAAGGCAG GGGTGATGGG TCAGGGGAAC CTACCCAGCC CCCTGAGGAC
    AGCTCTCCCC CTGCATCCTC TGAGAGTTCT TCCACCAGAG ATTCCGCGGT GGCCATTTCT
    GGAGCAGATT CCCGGGGAAT CCTGGAAGAG CCATTGCCTT CAACAAGCAG TGAAGAAGAA
    GATCCTCTTG CAGGTATTAG TCTCCCCGAA GGTGTGGACC CCTCTTTTCT GGCCGCTCTG
    CCTGATGACA TTCGCCGAGA AGTCCTACAG AACCAGCTGG GCATTCGTCC ACCAACACGA
    ACTGCCCCTT CAACAAATAA CTCAGCTCCT GCAGTGGTGG GGAATCCTGG TGTCACCGAA
    GTGAGCCCTG AGTTCCTGGC TGCTCTGCCT CCAGCCATCC AGGAGGAAGT ACTGGCACAG
    CAGAGAGCTG AGCAACAGCG ACGGGAACTA GCACAAAATG CCAGCTCAGA CACCCCCATG
    GACCCTGTGA CCTTCATCCA GACTCTGCCC TCAGACCTGC GCCGTAGTGT CCTAGAGGAC
    ATGGAGGACA GTGTGTTGGC CGTGATGCCA CCTGACATCG CAGCTGAAGC TCAAGCCCTG
    AGGCGAGAGC AAGAAGCCCG GCAGAGGCAG CTCATGCATG AGCGACTTTT TGGGCACAGC
    AGCACCTCTG CACTCTCTGC CATTCTCCGA AGCCCAGCCT TCACCAGTCG CCTGAGTGGC
    AACCGTGGGG TCCAATACAC TCGCCTTGCT GTGCAAAGAG GTGGCACCTT CCAGATGGGG
    GGTAGCAGCA GCCATAATAG GCCTTCTGGC AGTAATGTGG ACACCCTCCT ACGTCTTCGA
    GGACGGCTCC TCCTGGATCA CGAAGCCCTG TCTTGTCTCC TGGTCCTACT TTTTGTGGAT
    GAGCCAAAAC TCAATACTAG CCGTCTTCAC CGAGTACTGA GAAATCTCTG CTACCATGCC
    CAGACCCGCC ACTGGGTTAT CCGTAGCCTG CTTTCCATCT TGCAGCGAAG CAGTGAGAGT
    GAGCTTTGCA TTGAAACACC CAAGCTCTCC TCAAGTGAGG AAAAGGGCAA AAAGTCAAGC
    AAGAGCTGTG GGTCAAGCAG CCATGAGAAC CGGCCCCTGG ACCTGCTACA CAAGATGGAG
    TCTAAGAGTT CCAACCAGCT TTCCTGGCTC TCAGTATCCA TGGATGCAGC CCTAGGCTGC
    AGGACTAATA TATTCCAGAT CCAGCGTTCA GGGGGTCGCA AACATACTGA GAAACATGCG
    AGTAGTGGCT CCACTGTCCA TATCCACCCC CAGGCTGCTC CTGTTGTCTG CAGACATGTT
    CTGGATACAC TCATTCAATT GGCCAAGGTG TTTCCCAGTC ACTTCACACA GCAGCGGACC
    AAAGAAACAA ACTGTGAGAG TGATCGGGAG AGGGGCAGTA AGGCCTGCAG CCCATGCTCT
    TCACAGTCCA CAAGCAGTGG CATTTGCACA GACTTCTGGG ACTTATTGGT AAAACTGGAC
    AACATGAATG TCAGCCGGAA AGGCAAGAAC TCCGTGAAGT CAGTGCCAGT GAGCGCTGGT
    GGTGAGGGGG AAACCTCCCC ATACAGCCTC GAAGCTTCTC CACTGGGGCA ACTCATGAAC
    ATGTTGTCAC ACCCAGTCAT CCGCCGGAGC TCTCTCTTAA CTGAGAAGCT CCTCAGACTT
    CTTTCTCTCA TCTCAATTGC TCTCCCAGAA AACAAAGTGT CGGAAGCACA GGCTAATTCT
    GGCAGCAGTG CTTCCTCCAC CACTGTTGCC ACTTCAACCA CATCTACCAC CACTATCAGT
    GCCACCTCCA GCACATCTAC TCTGCCTGCT GCAACCACCC CTGTCACTTC TGTTCCAGCC
    CTGGTTGCTG CCACGGCTAT ATCCACCATT GCCGTAGCCG CTTCAACCAC AGTGACTACC
    CCCACAACTG CTACCACTAC TGTTTCAACT GCTACTACTA CTAAGGCCAG CAAATCTCCA
    GCTAAAGTTG GTGATATCGG CAGCAGCAGC AGCACAGACT TTAAGATGGT ATCCTCTGGC
    CTCACTGAAA ATCAGCTCCA GCTCTCTGTG GAAGTGTTGA CATCCCATTC TTGTTCTGAA
    GAAGGCCTAG AAGATGCAGC AAATGTGTTA CTGCAGCTCT CTCGGGGGGA CCCTGGGACC
    CGGGACACTG TTTTGAAGTT GCTACTGAAT GGAGCCCGCC ATCTGGGTTA TACGCTTTGT
    AAACAGATAG GAACTCTTCT GGCTGAGCTA CGGGAATACA ATCTGGAACA GCAGCGGCGA
    GCCCAGTGTG AAACCCTCTC CCCTGATGGC ATGCCTGAGG AACAGCCACA GACCACCAAG
    CTGAAAGGCA AAATGCAGAG CAGGTTTGAC GTGGCTGAGA ATGTGGTAAT CGTGGCATCT
    CAGAAGCGGC CTCTGGGTGG CCGGGAGCTC CAGCTGCCTT CTATGTCCAT GTTGACATCC
    AAGACATCTA CCCAGAAGTT CTTCTTGAGG GTACTGCAGG TCATCATCCA GCTCCGGGAT
    GACACGCGCC GTGCTAACAA GAAGGCCAAG CAGACAGGCA GGCTAGGTTC CTCCGGTTTA
    GGCTCAGCTA GCAGCATCCA GGCAGCTGTT CGGCAGCTGG AGGCTGAGGC TGATGCCATT
    ATACAAATGG TACGTGAGGG TCAGAGGGCG CGGAGACAGC AACAAGCAGC AACGTCGGAG
    TCAAGCCAGT CAGAGGCATC TGTCCGAAGG GAGGAGTCAC CCATGGATGT GGACCAGCCA
    TCTCCCAGTG CTCAAGATAC TCAGTCCATT GGCTCAGATG GAACTCCACA GGGAGAGAAG
    GAGAAGGAGG AGAGGCCACC TGAGTTACCC CTGCTCAGTG AGCAGCTGAA CTTGGATGAG
    CTGTGGGACA TGCTGGGAGA GTGTCTAAAG GAACTGGAGG AATCCCATGA CCAGCATGCG
    GTGCTAGTGC TACAACCTGC TGTCGAGGCC TTCTTTCTGG TCCATGCAAC AGAGCGGGAG
    AGCAAGCCTC CTGTCCGGGA CACCCGTGAG AGCCAGCTGG CACACATCAA GGACGAGCCA
    CCTCCACTCT CCCCTGCCCC TTTAACCCCA GCCACTCCTT CCTCCCTTGA CCCGTTCTTC
    TCCCGGGAGC CCTCATCTAT GCACATCTCC TCAAGCCTGC CCCCTGACAC ACAGAAGTTT
    CTTCGTTTTG CAGAGACTCA TCGCACTGTG TTAAACCAGA TCCTACGGCA GTCCACCACC
    CACCTCGCTG ATGGGCCTTT TGCTGTCCTG GTAGACTACA TTCGTGTCCT CGACTTCGAT
    GTCAAGCGCA AATATTTCCG CCAAGAGCTG GAGCGTTTGG ACGAGGGACT CCGGAAAGAA
    GACATGGCTG TGCACGTTCG TCGTGATCAT GTCTTTGAAG ACTCCTATCG TGAGCTGCAC
    CGGAAATCCC CTGAAGAAAT GAAGAATCGA TTGTATATAG TATTTGAAGG AGAAGAAGGG
    CAGGATGCTG GAGGGCTCCT GCGGGAGTGG TATATGATCA TCTCTCGAGA GATGTTTAAC
    CCTATGTATG CCTTGTTCCG TACCTCACCT GGTGATCGGG TCACATACAC CATCAATCCA
    TCTTCTCACT GCAACCCCAA CCACCTCAGC TACTTCAAGT TTGTTGGACG CATCGTGGCC
    AAAGCTGTGT ATGACAACCG CCTCCTGGAG TGCTACTTTA CTAGGTCCTT CTACAAACAC
    ATTTTGGGCA AGTCTGTCAG ATATACAGAT ATGGAGAGTG AAGACTACCA CTTTTACCAG
    GGCCTGGTTT ATCTGCTGGA AAATGATGTC TCCACACTAG GCTATGATCT CACTTTCAGC
    ACTGAGGTCC AAGAGTTTGG AGTCTGTGAA GTTCGTGACC TCAAACCCAA TGGGGCCAAC
    ATCTTGGTAA CAGAGGAAAA TAAGAAGGAA TATGTACACC TGGTGTGCCA GATGAGAATG
    ACAGGAGCCA TCCGCAAACA GCTGGCGGCT TTCTTAGAAG GTTTCTATGA GATCATTCCA
    AAGCGCCTCA TTTCCATCTT CACTGAGCAG GAGTTAGAGC TGCTCATATC AGGACTGCCC
    ACAATCGACA TTGACGATTT GAAATCTAAC ACTGAATATC ACAAGTACCA GTCCAACTCT
    ATTCAGATCC AGTGGTTCTG GAGAGCACTG CGTTCTTTCG ATCAAGCTGA CCGTGCCAAG
    TTCCTCCAGT TTGTCACAGG TACTTCCAAG GTGCCCCTGC AAGGTTTTGC TGCCCTTGAA
    GGCATGAATG GCATTCAGAA GTTTCAAATC CATCGAGATG ACAGGTCCAC AGACCGCCTG
    CCTTCAGCTC ACACATGTTT TAATCAGCTG GACCTGCCTG CCTATGAGAG CTTTGAGAAG
    CTCCGCCACA TGCTACTATT GGCCATCCAG GAGTGCTCTG AAGGCTTTGG GCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48121
    Clone ID Related Accession (Same CDS sequence) XM_024987896.1 , XM_010821999.2
    Accession Version XM_024987896.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGTCGGAG TCAAGCCAGT CAGAGGCATC TGTCCGAAGG
    GAGGAGTCAC CCATGGATGT GGACCAGCCA TCTCCCAGTG CTCAAGATAC TCAGTCCATT
    GGCTCAGATG GAACTCCACA GGGAGAGAAG GAGAAGGAGG AGAGGCCACC TGAGTTACCC
    CTGCTCAGTG AGCAGCTGAA CTTGGATGAG CTGTGGGACA TGCTGGGAGA GTGTCTAAAG
    GAACTGGAGG AATCCCATGA CCAGCATGCG GTGCTAGTGC TACAACCTGC TGTCGAGGCC
    TTCTTTCTGG TCCATGCAAC AGAGCGGGAG AGCAAGCCTC CTGTCCGGGA CACCCGTGAG
    AGCCAGCTGG CACACATCAA GGACGAGCCA CCTCCACTCT CCCCTGCCCC TTTAACCCCA
    GCCACTCCTT CCTCCCTTGA CCCGTTCTTC TCCCGGGAGC CCTCATCTAT GCACATCTCC
    TCAAGCCTGC CCCCTGACAC ACAGAAGTTT CTTCGTTTTG CAGAGACTCA TCGCACTGTG
    TTAAACCAGA TCCTACGGCA GTCCACCACC CACCTCGCTG ATGGGCCTTT TGCTGTCCTG
    GTAGACTACA TTCGTGTCCT CGACTTCGAT GTCAAGCGCA AATATTTCCG CCAAGAGCTG
    GAGCGTTTGG ACGAGGGACT CCGGAAAGAA GACATGGCTG TGCACGTTCG TCGTGATCAT
    GTCTTTGAAG ACTCCTATCG TGAGCTGCAC CGGAAATCCC CTGAAGAAAT GAAGAATCGA
    TTGTATATAG TATTTGAAGG AGAAGAAGGG CAGGATGCTG GAGGGCTCCT GCGGGAGTGG
    TATATGATCA TCTCTCGAGA GATGTTTAAC CCTATGTATG CCTTGTTCCG TACCTCACCT
    GGTGATCGGG TCACATACAC CATCAATCCA TCTTCTCACT GCAACCCCAA CCACCTCAGC
    TACTTCAAGT TTGTTGGACG CATCGTGGCC AAAGCTGTGT ATGACAACCG CCTCCTGGAG
    TGCTACTTTA CTAGGTCCTT CTACAAACAC ATTTTGGGCA AGTCTGTCAG ATATACAGAT
    ATGGAGAGTG AAGACTACCA CTTTTACCAG GGCCTGGTTT ATCTGCTGGA AAATGATGTC
    TCCACACTAG GCTATGATCT CACTTTCAGC ACTGAGGTCC AAGAGTTTGG AGTCTGTGAA
    GTTCGTGACC TCAAACCCAA TGGGGCCAAC ATCTTGGTAA CAGAGGAAAA TAAGAAGGAA
    TATGTACACC TGGTGTGCCA GATGAGAATG ACAGGAGCCA TCCGCAAACA GCTGGCGGCT
    TTCTTAGAAG GTTTCTATGA GATCATTCCA AAGCGCCTCA TTTCCATCTT CACTGAGCAG
    GAGTTAGAGC TGCTCATATC AGGACTGCCC ACAATCGACA TTGACGATTT GAAATCTAAC
    ACTGAATATC ACAAGTACCA GTCCAACTCT ATTCAGATCC AGTGGTTCTG GAGAGCACTG
    CGTTCTTTCG ATCAAGCTGA CCGTGCCAAG TTCCTCCAGT TTGTCACAGG TACTTCCAAG
    GTGCCCCTGC AAGGTTTTGC TGCCCTTGAA GGCATGAATG GCATTCAGAA GTTTCAAATC
    CATCGAGATG ACAGGTCCAC AGACCGCCTG CCTTCAGCTC ACACATGTTT TAATCAGCTG
    GACCTGCCTG CCTATGAGAG CTTTGAGAAG CTCCGCCACA TGCTACTATT GGCCATCCAG
    GAGTGCTCTG AAGGCTTTGG GCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843664.1
    CDS442..13671
    Translation

    Target ORF information:

    RefSeq Version XM_024987896.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987896.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGTCGGAG TCAAGCCAGT CAGAGGCATC TGTCCGAAGG
    GAGGAGTCAC CCATGGATGT GGACCAGCCA TCTCCCAGTG CTCAAGATAC TCAGTCCATT
    GGCTCAGATG GAACTCCACA GGGAGAGAAG GAGAAGGAGG AGAGGCCACC TGAGTTACCC
    CTGCTCAGTG AGCAGCTGAA CTTGGATGAG CTGTGGGACA TGCTGGGAGA GTGTCTAAAG
    GAACTGGAGG AATCCCATGA CCAGCATGCG GTGCTAGTGC TACAACCTGC TGTCGAGGCC
    TTCTTTCTGG TCCATGCAAC AGAGCGGGAG AGCAAGCCTC CTGTCCGGGA CACCCGTGAG
    AGCCAGCTGG CACACATCAA GGACGAGCCA CCTCCACTCT CCCCTGCCCC TTTAACCCCA
    GCCACTCCTT CCTCCCTTGA CCCGTTCTTC TCCCGGGAGC CCTCATCTAT GCACATCTCC
    TCAAGCCTGC CCCCTGACAC ACAGAAGTTT CTTCGTTTTG CAGAGACTCA TCGCACTGTG
    TTAAACCAGA TCCTACGGCA GTCCACCACC CACCTCGCTG ATGGGCCTTT TGCTGTCCTG
    GTAGACTACA TTCGTGTCCT CGACTTCGAT GTCAAGCGCA AATATTTCCG CCAAGAGCTG
    GAGCGTTTGG ACGAGGGACT CCGGAAAGAA GACATGGCTG TGCACGTTCG TCGTGATCAT
    GTCTTTGAAG ACTCCTATCG TGAGCTGCAC CGGAAATCCC CTGAAGAAAT GAAGAATCGA
    TTGTATATAG TATTTGAAGG AGAAGAAGGG CAGGATGCTG GAGGGCTCCT GCGGGAGTGG
    TATATGATCA TCTCTCGAGA GATGTTTAAC CCTATGTATG CCTTGTTCCG TACCTCACCT
    GGTGATCGGG TCACATACAC CATCAATCCA TCTTCTCACT GCAACCCCAA CCACCTCAGC
    TACTTCAAGT TTGTTGGACG CATCGTGGCC AAAGCTGTGT ATGACAACCG CCTCCTGGAG
    TGCTACTTTA CTAGGTCCTT CTACAAACAC ATTTTGGGCA AGTCTGTCAG ATATACAGAT
    ATGGAGAGTG AAGACTACCA CTTTTACCAG GGCCTGGTTT ATCTGCTGGA AAATGATGTC
    TCCACACTAG GCTATGATCT CACTTTCAGC ACTGAGGTCC AAGAGTTTGG AGTCTGTGAA
    GTTCGTGACC TCAAACCCAA TGGGGCCAAC ATCTTGGTAA CAGAGGAAAA TAAGAAGGAA
    TATGTACACC TGGTGTGCCA GATGAGAATG ACAGGAGCCA TCCGCAAACA GCTGGCGGCT
    TTCTTAGAAG GTTTCTATGA GATCATTCCA AAGCGCCTCA TTTCCATCTT CACTGAGCAG
    GAGTTAGAGC TGCTCATATC AGGACTGCCC ACAATCGACA TTGACGATTT GAAATCTAAC
    ACTGAATATC ACAAGTACCA GTCCAACTCT ATTCAGATCC AGTGGTTCTG GAGAGCACTG
    CGTTCTTTCG ATCAAGCTGA CCGTGCCAAG TTCCTCCAGT TTGTCACAGG TACTTCCAAG
    GTGCCCCTGC AAGGTTTTGC TGCCCTTGAA GGCATGAATG GCATTCAGAA GTTTCAAATC
    CATCGAGATG ACAGGTCCAC AGACCGCCTG CCTTCAGCTC ACACATGTTT TAATCAGCTG
    GACCTGCCTG CCTATGAGAG CTTTGAGAAG CTCCGCCACA TGCTACTATT GGCCATCCAG
    GAGTGCTCTG AAGGCTTTGG GCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48121
    Clone ID Related Accession (Same CDS sequence) XM_024987896.1 , XM_010821999.2
    Accession Version XM_010821999.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821999.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGTCGGAG TCAAGCCAGT CAGAGGCATC TGTCCGAAGG
    GAGGAGTCAC CCATGGATGT GGACCAGCCA TCTCCCAGTG CTCAAGATAC TCAGTCCATT
    GGCTCAGATG GAACTCCACA GGGAGAGAAG GAGAAGGAGG AGAGGCCACC TGAGTTACCC
    CTGCTCAGTG AGCAGCTGAA CTTGGATGAG CTGTGGGACA TGCTGGGAGA GTGTCTAAAG
    GAACTGGAGG AATCCCATGA CCAGCATGCG GTGCTAGTGC TACAACCTGC TGTCGAGGCC
    TTCTTTCTGG TCCATGCAAC AGAGCGGGAG AGCAAGCCTC CTGTCCGGGA CACCCGTGAG
    AGCCAGCTGG CACACATCAA GGACGAGCCA CCTCCACTCT CCCCTGCCCC TTTAACCCCA
    GCCACTCCTT CCTCCCTTGA CCCGTTCTTC TCCCGGGAGC CCTCATCTAT GCACATCTCC
    TCAAGCCTGC CCCCTGACAC ACAGAAGTTT CTTCGTTTTG CAGAGACTCA TCGCACTGTG
    TTAAACCAGA TCCTACGGCA GTCCACCACC CACCTCGCTG ATGGGCCTTT TGCTGTCCTG
    GTAGACTACA TTCGTGTCCT CGACTTCGAT GTCAAGCGCA AATATTTCCG CCAAGAGCTG
    GAGCGTTTGG ACGAGGGACT CCGGAAAGAA GACATGGCTG TGCACGTTCG TCGTGATCAT
    GTCTTTGAAG ACTCCTATCG TGAGCTGCAC CGGAAATCCC CTGAAGAAAT GAAGAATCGA
    TTGTATATAG TATTTGAAGG AGAAGAAGGG CAGGATGCTG GAGGGCTCCT GCGGGAGTGG
    TATATGATCA TCTCTCGAGA GATGTTTAAC CCTATGTATG CCTTGTTCCG TACCTCACCT
    GGTGATCGGG TCACATACAC CATCAATCCA TCTTCTCACT GCAACCCCAA CCACCTCAGC
    TACTTCAAGT TTGTTGGACG CATCGTGGCC AAAGCTGTGT ATGACAACCG CCTCCTGGAG
    TGCTACTTTA CTAGGTCCTT CTACAAACAC ATTTTGGGCA AGTCTGTCAG ATATACAGAT
    ATGGAGAGTG AAGACTACCA CTTTTACCAG GGCCTGGTTT ATCTGCTGGA AAATGATGTC
    TCCACACTAG GCTATGATCT CACTTTCAGC ACTGAGGTCC AAGAGTTTGG AGTCTGTGAA
    GTTCGTGACC TCAAACCCAA TGGGGCCAAC ATCTTGGTAA CAGAGGAAAA TAAGAAGGAA
    TATGTACACC TGGTGTGCCA GATGAGAATG ACAGGAGCCA TCCGCAAACA GCTGGCGGCT
    TTCTTAGAAG GTTTCTATGA GATCATTCCA AAGCGCCTCA TTTCCATCTT CACTGAGCAG
    GAGTTAGAGC TGCTCATATC AGGACTGCCC ACAATCGACA TTGACGATTT GAAATCTAAC
    ACTGAATATC ACAAGTACCA GTCCAACTCT ATTCAGATCC AGTGGTTCTG GAGAGCACTG
    CGTTCTTTCG ATCAAGCTGA CCGTGCCAAG TTCCTCCAGT TTGTCACAGG TACTTCCAAG
    GTGCCCCTGC AAGGTTTTGC TGCCCTTGAA GGCATGAATG GCATTCAGAA GTTTCAAATC
    CATCGAGATG ACAGGTCCAC AGACCGCCTG CCTTCAGCTC ACACATGTTT TAATCAGCTG
    GACCTGCCTG CCTATGAGAG CTTTGAGAAG CTCCGCCACA TGCTACTATT GGCCATCCAG
    GAGTGCTCTG AAGGCTTTGG GCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820301.1
    CDS735..13964
    Translation

    Target ORF information:

    RefSeq Version XM_010821999.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821999.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGTCGGAG TCAAGCCAGT CAGAGGCATC TGTCCGAAGG
    GAGGAGTCAC CCATGGATGT GGACCAGCCA TCTCCCAGTG CTCAAGATAC TCAGTCCATT
    GGCTCAGATG GAACTCCACA GGGAGAGAAG GAGAAGGAGG AGAGGCCACC TGAGTTACCC
    CTGCTCAGTG AGCAGCTGAA CTTGGATGAG CTGTGGGACA TGCTGGGAGA GTGTCTAAAG
    GAACTGGAGG AATCCCATGA CCAGCATGCG GTGCTAGTGC TACAACCTGC TGTCGAGGCC
    TTCTTTCTGG TCCATGCAAC AGAGCGGGAG AGCAAGCCTC CTGTCCGGGA CACCCGTGAG
    AGCCAGCTGG CACACATCAA GGACGAGCCA CCTCCACTCT CCCCTGCCCC TTTAACCCCA
    GCCACTCCTT CCTCCCTTGA CCCGTTCTTC TCCCGGGAGC CCTCATCTAT GCACATCTCC
    TCAAGCCTGC CCCCTGACAC ACAGAAGTTT CTTCGTTTTG CAGAGACTCA TCGCACTGTG
    TTAAACCAGA TCCTACGGCA GTCCACCACC CACCTCGCTG ATGGGCCTTT TGCTGTCCTG
    GTAGACTACA TTCGTGTCCT CGACTTCGAT GTCAAGCGCA AATATTTCCG CCAAGAGCTG
    GAGCGTTTGG ACGAGGGACT CCGGAAAGAA GACATGGCTG TGCACGTTCG TCGTGATCAT
    GTCTTTGAAG ACTCCTATCG TGAGCTGCAC CGGAAATCCC CTGAAGAAAT GAAGAATCGA
    TTGTATATAG TATTTGAAGG AGAAGAAGGG CAGGATGCTG GAGGGCTCCT GCGGGAGTGG
    TATATGATCA TCTCTCGAGA GATGTTTAAC CCTATGTATG CCTTGTTCCG TACCTCACCT
    GGTGATCGGG TCACATACAC CATCAATCCA TCTTCTCACT GCAACCCCAA CCACCTCAGC
    TACTTCAAGT TTGTTGGACG CATCGTGGCC AAAGCTGTGT ATGACAACCG CCTCCTGGAG
    TGCTACTTTA CTAGGTCCTT CTACAAACAC ATTTTGGGCA AGTCTGTCAG ATATACAGAT
    ATGGAGAGTG AAGACTACCA CTTTTACCAG GGCCTGGTTT ATCTGCTGGA AAATGATGTC
    TCCACACTAG GCTATGATCT CACTTTCAGC ACTGAGGTCC AAGAGTTTGG AGTCTGTGAA
    GTTCGTGACC TCAAACCCAA TGGGGCCAAC ATCTTGGTAA CAGAGGAAAA TAAGAAGGAA
    TATGTACACC TGGTGTGCCA GATGAGAATG ACAGGAGCCA TCCGCAAACA GCTGGCGGCT
    TTCTTAGAAG GTTTCTATGA GATCATTCCA AAGCGCCTCA TTTCCATCTT CACTGAGCAG
    GAGTTAGAGC TGCTCATATC AGGACTGCCC ACAATCGACA TTGACGATTT GAAATCTAAC
    ACTGAATATC ACAAGTACCA GTCCAACTCT ATTCAGATCC AGTGGTTCTG GAGAGCACTG
    CGTTCTTTCG ATCAAGCTGA CCGTGCCAAG TTCCTCCAGT TTGTCACAGG TACTTCCAAG
    GTGCCCCTGC AAGGTTTTGC TGCCCTTGAA GGCATGAATG GCATTCAGAA GTTTCAAATC
    CATCGAGATG ACAGGTCCAC AGACCGCCTG CCTTCAGCTC ACACATGTTT TAATCAGCTG
    GACCTGCCTG CCTATGAGAG CTTTGAGAAG CTCCGCCACA TGCTACTATT GGCCATCCAG
    GAGTGCTCTG AAGGCTTTGG GCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48122
    Clone ID Related Accession (Same CDS sequence) XM_024987897.1
    Accession Version XM_024987897.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
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    9301
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    9781
    9841
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    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGATTCCATG
    AATATCCTGG ACCCTGAGGA TGAGGAGGAG CACACTCAGG AAGAGGACAG CAGTGGCAGT
    AACGAGGATG AGGATGATAG TCAGGATGAA GAGGAGGAGG AGGAGGAAGA TGAGGAAGAT
    GATCAGGAGG ATGATGAAGG TGAAGAGGGA GATGAAGACG ATGACGACGA TGGCTCTGAA
    ATGGAATTGG ATGAGGATTA CCCTGATATG AACGCTTCTC CCTTGGTCCG ATTTGAGCGC
    TTTGACCGGG AGGATGATCT CATCATTGAG TTTGACAACA TGTTCTCCAG TGCTACAGAC
    ATCCCCCCAT CCCCAGGAAA TATCCCTACC ACCCACCCAC TGATGGTACG CCATGCAGAT
    CACAGTTCCC TGACACTGGG CAGTGGCTCC TCTACAACAC GTCTCACCCA GGGCATTGGG
    CGCAGTCAAA GGACCCTGAG ACAGCTGACA GCCAACACAG GCCATACCAT TCACGTTCAC
    TACCCTGGGA ATCGCCAGCC CAATCCTCCT CTTATACTCC AGAGGTTGCT TGGTCCCTCT
    GCTGCTGCTG ACATCCTTCA GCTGAGCAGC AGCCTTCCCC TACAAAGCCG AGGCCGAGCC
    CGCCTCCTAG TGGGCAACGA TGACGTCCAC ATCATCGCCC GGTCTGATGA TGAACTACTG
    GATGACTTTT TCCATGATCA GAGCACAGCT ACCAGCCAAG CAGGGACCCT ATCCAGTATC
    CCTACAGCCC TGACTCGCTG GACAGAGGAA TGCAAAGTTC TTGATGCTGA GAGCATGCAT
    GACTGTGTTT CAGTTGTTAA AGTGCCCATT GTCAATCATC TGGAATTCCT GAGGGATGAG
    GAGCTGGAAG AAAGGAGGGA GAAAAGGAGG AAACAACTAG CTGAGGAAGA AACAAAGATA
    ACCGACAAAG GCAAAGAAGA TAAGGAGAAC AGGGATCAGA GTGCACAGTG TGCTGCATCT
    AAGACAAATG ACTCCACTGA ACAGAACCTC TCAGATGGGA CTCCTATGCC TGACAGCTAC
    CCAGCAACCC CATCTTCAAC TGATGTAGCT ACATCTGAGC CCAGGGAGAC CCTTGTCACT
    CTGCAGCCCT CCCAACAGCA ACCAACACTC CCAACCCCAC CAGCCTTGGG AGAGATTCCT
    CAAGAGCTGC AGTCCCCAAT TGGAGAAGGT GGGAGCTCTA CACAGCTATT GATGCCTGTA
    GAGCCAGAGG AATTGGGTCC CACAAGGCCA AGTGGAGAAG CAGAAACAAC TCAGATGGAG
    CTATCTCCAG CTCCCACTAT AACCTCTCTT TCCCCAGAGA GAGCTGAAGA TTCTGATGCT
    CTGACAGCTG TCAGCAGTCA ACTGGAAGGC TCTCCCATGG ATACTAGCAG CCTGGCTTCC
    TGTACCCTGG AGGAGGCTGT GGGTGACGCC CCAGCAACTG GCAGTTCTGA GCAGCCCACA
    GCAGGCAGCT CCACTCCTGG GGATGCCCCA CCAGTTGTGA CAGAAGTACA AGGCAGGGGT
    GATGGGTCAG GGGAACCTAC CCAGCCCCCT GAGGACAGCT CTCCCCCTGC ATCCTCTGAG
    AGTTCTTCCA CCAGAGATTC CGCGGTGGCC ATTTCTGGAG CAGATTCCCG GGGAATCCTG
    GAAGAGCCAT TGCCTTCAAC AAGCAGTGAA GAAGAAGATC CTCTTGCAGG TATTAGTCTC
    CCCGAAGGTG TGGACCCCTC TTTTCTGGCC GCTCTGCCTG ATGACATTCG CCGAGAAGTC
    CTACAGAACC AGCTGGGCAT TCGTCCACCA ACACGAACTG CCCCTTCAAC AAATAACTCA
    GCTCCTGCAG TGGTGGGGAA TCCTGGTGTC ACCGAAGTGA GCCCTGAGTT CCTGGCTGCT
    CTGCCTCCAG CCATCCAGGA GGAAGTACTG GCACAGCAGA GAGCTGAGCA ACAGCGACGG
    GAACTAGCAC AAAATGCCAG CTCAGACACC CCCATGGACC CTGTGACCTT CATCCAGACT
    CTGCCCTCAG ACCTGCGCCG TAGTGTCCTA GAGGACATGG AGGACAGTGT GTTGGCCGTG
    ATGCCACCTG ACATCGCAGC TGAAGCTCAA GCCCTGAGGC GAGAGCAAGA AGCCCGGCAG
    AGGCAGCTCA TGCATGAGCG ACTTTTTGGG CACAGCAGCA CCTCTGCACT CTCTGCCATT
    CTCCGAAGCC CAGCCTTCAC CAGTCGCCTG AGTGGCAACC GTGGGGTCCA ATACACTCGC
    CTTGCTGTGC AAAGAGGTGG CACCTTCCAG ATGGGGGGTA GCAGCAGCCA TAATAGGCCT
    TCTGGCAGTA ATGTGGACAC CCTCCTACGT CTTCGAGGAC GGCTCCTCCT GGATCACGAA
    GCCCTGTCTT GTCTCCTGGT CCTACTTTTT GTGGATGAGC CAAAACTCAA TACTAGCCGT
    CTTCACCGAG TACTGAGAAA TCTCTGCTAC CATGCCCAGA CCCGCCACTG GGTTATCCGT
    AGCCTGCTTT CCATCTTGCA GCGAAGCAGT GAGAGTGAGC TTTGCATTGA AACACCCAAG
    CTCTCCTCAA GTGAGGAAAA GGGCAAAAAG TCAAGCAAGA GCTGTGGGTC AAGCAGCCAT
    GAGAACCGGC CCCTGGACCT GCTACACAAG ATGGAGTCTA AGAGTTCCAA CCAGCTTTCC
    TGGCTCTCAG TATCCATGGA TGCAGCCCTA GGCTGCAGGA CTAATATATT CCAGATCCAG
    CGTTCAGGGG GTCGCAAACA TACTGAGAAA CATGCGAGTA GTGGCTCCAC TGTCCATATC
    CACCCCCAGG CTGCTCCTGT TGTCTGCAGA CATGTTCTGG ATACACTCAT TCAATTGGCC
    AAGGTGTTTC CCAGTCACTT CACACAGCAG CGGACCAAAG AAACAAACTG TGAGAGTGAT
    CGGGAGAGGG GCAGTAAGGC CTGCAGCCCA TGCTCTTCAC AGTCCACAAG CAGTGGCATT
    TGCACAGACT TCTGGGACTT ATTGGTAAAA CTGGACAACA TGAATGTCAG CCGGAAAGGC
    AAGAACTCCG TGAAGTCAGT GCCAGTGAGC GCTGGTGGTG AGGGGGAAAC CTCCCCATAC
    AGCCTCGAAG CTTCTCCACT GGGGCAACTC ATGAACATGT TGTCACACCC AGTCATCCGC
    CGGAGCTCTC TCTTAACTGA GAAGCTCCTC AGACTTCTTT CTCTCATCTC AATTGCTCTC
    CCAGAAAACA AAGTGTCGGA AGCACAGGCT AATTCTGGCA GCAGTGCTTC CTCCACCACT
    GTTGCCACTT CAACCACATC TACCACCACT ATCAGTGCCA CCTCCAGCAC ATCTACTCTG
    CCTGCTGCAA CCACCCCTGT CACTTCTGTT CCAGCCCTGG TTGCTGCCAC GGCTATATCC
    ACCATTGCCG TAGCCGCTTC AACCACAGTG ACTACCCCCA CAACTGCTAC CACTACTGTT
    TCAACTGCTA CTACTACTAA GGCCAGCAAA TCTCCAGCTA AAGTTGGTGA TATCGGCAGC
    AGCAGCAGCA CAGACTTTAA GATGGTATCC TCTGGCCTCA CTGAAAATCA GCTCCAGCTC
    TCTGTGGAAG TGTTGACATC CCATTCTTGT TCTGAAGAAG GCCTAGAAGA TGCAGCAAAT
    GTGTTACTGC AGCTCTCTCG GGGGGACCCT GGGACCCGGG ACACTGTTTT GAAGTTGCTA
    CTGAATGGAG CCCGCCATCT GGGTTATACG CTTTGTAAAC AGATAGGAAC TCTTCTGGCT
    GAGCTACGGG AATACAATCT GGAACAGCAG CGGCGAGCCC AGTGTGAAAC CCTCTCCCCT
    GATGGCATGC CTGAGGAACA GCCACAGACC ACCAAGCTGA AAGGCAAAAT GCAGAGCAGG
    TTTGACGTGG CTGAGAATGT GGTAATCGTG GCATCTCAGA AGCGGCCTCT GGGTGGCCGG
    GAGCTCCAGC TGCCTTCTAT GTCCATGTTG ACATCCAAGA CATCTACCCA GAAGTTCTTC
    TTGAGGGTAC TGCAGGTCAT CATCCAGCTC CGGGATGACA CGCGCCGTGC TAACAAGAAG
    GCCAAGCAGA CAGGCAGGCT AGGTTCCTCC GGTTTAGGCT CAGCTAGCAG CATCCAGGCA
    GCTGTTCGGC AGCTGGAGGC TGAGGCTGAT GCCATTATAC AAATGGTACG TGAGGGTCAG
    AGGGCGCGGA GACAGCAACA AGCAGCAACG TCGGAGTCAA GCCAGTCAGA GGCATCTGTC
    CGAAGGGAGG AGTCACCCAT GGATGTGGAC CAGCCATCTC CCAGTGCTCA AGATACTCAG
    TCCATTGGCT CAGATGGAAC TCCACAGGGA GAGAAGGAGA AGGAGGAGAG GCCACCTGAG
    TTACCCCTGC TCAGTGAGCA GCTGAACTTG GATGAGCTGT GGGACATGCT GGGAGAGTGT
    CTAAAGGAAC TGGAGGAATC CCATGACCAG CATGCGGTGC TAGTGCTACA ACCTGCTGTC
    GAGGCCTTCT TTCTGGTCCA TGCAACAGAG CGGGAGAGCA AGCCTCCTGT CCGGGACACC
    CGTGAGAGCC AGCTGGCACA CATCAAGGAC GAGCCACCTC CACTCTCCCC TGCCCCTTTA
    ACCCCAGCCA CTCCTTCCTC CCTTGACCCG TTCTTCTCCC GGGAGCCCTC ATCTATGCAC
    ATCTCCTCAA GCCTGCCCCC TGACACACAG AAGTTTCTTC GTTTTGCAGA GACTCATCGC
    ACTGTGTTAA ACCAGATCCT ACGGCAGTCC ACCACCCACC TCGCTGATGG GCCTTTTGCT
    GTCCTGGTAG ACTACATTCG TGTCCTCGAC TTCGATGTCA AGCGCAAATA TTTCCGCCAA
    GAGCTGGAGC GTTTGGACGA GGGACTCCGG AAAGAAGACA TGGCTGTGCA CGTTCGTCGT
    GATCATGTCT TTGAAGACTC CTATCGTGAG CTGCACCGGA AATCCCCTGA AGAAATGAAG
    AATCGATTGT ATATAGTATT TGAAGGAGAA GAAGGGCAGG ATGCTGGAGG GCTCCTGCGG
    GAGTGGTATA TGATCATCTC TCGAGAGATG TTTAACCCTA TGTATGCCTT GTTCCGTACC
    TCACCTGGTG ATCGGGTCAC ATACACCATC AATCCATCTT CTCACTGCAA CCCCAACCAC
    CTCAGCTACT TCAAGTTTGT TGGACGCATC GTGGCCAAAG CTGTGTATGA CAACCGCCTC
    CTGGAGTGCT ACTTTACTAG GTCCTTCTAC AAACACATTT TGGGCAAGTC TGTCAGATAT
    ACAGATATGG AGAGTGAAGA CTACCACTTT TACCAGGGCC TGGTTTATCT GCTGGAAAAT
    GATGTCTCCA CACTAGGCTA TGATCTCACT TTCAGCACTG AGGTCCAAGA GTTTGGAGTC
    TGTGAAGTTC GTGACCTCAA ACCCAATGGG GCCAACATCT TGGTAACAGA GGAAAATAAG
    AAGGAATATG TACACCTGGT GTGCCAGATG AGAATGACAG GAGCCATCCG CAAACAGCTG
    GCGGCTTTCT TAGAAGGTTT CTATGAGATC ATTCCAAAGC GCCTCATTTC CATCTTCACT
    GAGCAGGAGT TAGAGCTGCT CATATCAGGA CTGCCCACAA TCGACATTGA CGATTTGAAA
    TCTAACACTG AATATCACAA GTACCAGTCC AACTCTATTC AGATCCAGTG GTTCTGGAGA
    GCACTGCGTT CTTTCGATCA AGCTGACCGT GCCAAGTTCC TCCAGTTTGT CACAGGTACT
    TCCAAGGTGC CCCTGCAAGG TTTTGCTGCC CTTGAAGGCA TGAATGGCAT TCAGAAGTTT
    CAAATCCATC GAGATGACAG GTCCACAGAC CGCCTGCCTT CAGCTCACAC ATGTTTTAAT
    CAGCTGGACC TGCCTGCCTA TGAGAGCTTT GAGAAGCTCC GCCACATGCT ACTATTGGCC
    ATCCAGGAGT GCTCTGAAGG CTTTGGGCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843665.1
    CDS442..13617
    Translation

    Target ORF information:

    RefSeq Version XM_024987897.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987897.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGATTCCATG
    AATATCCTGG ACCCTGAGGA TGAGGAGGAG CACACTCAGG AAGAGGACAG CAGTGGCAGT
    AACGAGGATG AGGATGATAG TCAGGATGAA GAGGAGGAGG AGGAGGAAGA TGAGGAAGAT
    GATCAGGAGG ATGATGAAGG TGAAGAGGGA GATGAAGACG ATGACGACGA TGGCTCTGAA
    ATGGAATTGG ATGAGGATTA CCCTGATATG AACGCTTCTC CCTTGGTCCG ATTTGAGCGC
    TTTGACCGGG AGGATGATCT CATCATTGAG TTTGACAACA TGTTCTCCAG TGCTACAGAC
    ATCCCCCCAT CCCCAGGAAA TATCCCTACC ACCCACCCAC TGATGGTACG CCATGCAGAT
    CACAGTTCCC TGACACTGGG CAGTGGCTCC TCTACAACAC GTCTCACCCA GGGCATTGGG
    CGCAGTCAAA GGACCCTGAG ACAGCTGACA GCCAACACAG GCCATACCAT TCACGTTCAC
    TACCCTGGGA ATCGCCAGCC CAATCCTCCT CTTATACTCC AGAGGTTGCT TGGTCCCTCT
    GCTGCTGCTG ACATCCTTCA GCTGAGCAGC AGCCTTCCCC TACAAAGCCG AGGCCGAGCC
    CGCCTCCTAG TGGGCAACGA TGACGTCCAC ATCATCGCCC GGTCTGATGA TGAACTACTG
    GATGACTTTT TCCATGATCA GAGCACAGCT ACCAGCCAAG CAGGGACCCT ATCCAGTATC
    CCTACAGCCC TGACTCGCTG GACAGAGGAA TGCAAAGTTC TTGATGCTGA GAGCATGCAT
    GACTGTGTTT CAGTTGTTAA AGTGCCCATT GTCAATCATC TGGAATTCCT GAGGGATGAG
    GAGCTGGAAG AAAGGAGGGA GAAAAGGAGG AAACAACTAG CTGAGGAAGA AACAAAGATA
    ACCGACAAAG GCAAAGAAGA TAAGGAGAAC AGGGATCAGA GTGCACAGTG TGCTGCATCT
    AAGACAAATG ACTCCACTGA ACAGAACCTC TCAGATGGGA CTCCTATGCC TGACAGCTAC
    CCAGCAACCC CATCTTCAAC TGATGTAGCT ACATCTGAGC CCAGGGAGAC CCTTGTCACT
    CTGCAGCCCT CCCAACAGCA ACCAACACTC CCAACCCCAC CAGCCTTGGG AGAGATTCCT
    CAAGAGCTGC AGTCCCCAAT TGGAGAAGGT GGGAGCTCTA CACAGCTATT GATGCCTGTA
    GAGCCAGAGG AATTGGGTCC CACAAGGCCA AGTGGAGAAG CAGAAACAAC TCAGATGGAG
    CTATCTCCAG CTCCCACTAT AACCTCTCTT TCCCCAGAGA GAGCTGAAGA TTCTGATGCT
    CTGACAGCTG TCAGCAGTCA ACTGGAAGGC TCTCCCATGG ATACTAGCAG CCTGGCTTCC
    TGTACCCTGG AGGAGGCTGT GGGTGACGCC CCAGCAACTG GCAGTTCTGA GCAGCCCACA
    GCAGGCAGCT CCACTCCTGG GGATGCCCCA CCAGTTGTGA CAGAAGTACA AGGCAGGGGT
    GATGGGTCAG GGGAACCTAC CCAGCCCCCT GAGGACAGCT CTCCCCCTGC ATCCTCTGAG
    AGTTCTTCCA CCAGAGATTC CGCGGTGGCC ATTTCTGGAG CAGATTCCCG GGGAATCCTG
    GAAGAGCCAT TGCCTTCAAC AAGCAGTGAA GAAGAAGATC CTCTTGCAGG TATTAGTCTC
    CCCGAAGGTG TGGACCCCTC TTTTCTGGCC GCTCTGCCTG ATGACATTCG CCGAGAAGTC
    CTACAGAACC AGCTGGGCAT TCGTCCACCA ACACGAACTG CCCCTTCAAC AAATAACTCA
    GCTCCTGCAG TGGTGGGGAA TCCTGGTGTC ACCGAAGTGA GCCCTGAGTT CCTGGCTGCT
    CTGCCTCCAG CCATCCAGGA GGAAGTACTG GCACAGCAGA GAGCTGAGCA ACAGCGACGG
    GAACTAGCAC AAAATGCCAG CTCAGACACC CCCATGGACC CTGTGACCTT CATCCAGACT
    CTGCCCTCAG ACCTGCGCCG TAGTGTCCTA GAGGACATGG AGGACAGTGT GTTGGCCGTG
    ATGCCACCTG ACATCGCAGC TGAAGCTCAA GCCCTGAGGC GAGAGCAAGA AGCCCGGCAG
    AGGCAGCTCA TGCATGAGCG ACTTTTTGGG CACAGCAGCA CCTCTGCACT CTCTGCCATT
    CTCCGAAGCC CAGCCTTCAC CAGTCGCCTG AGTGGCAACC GTGGGGTCCA ATACACTCGC
    CTTGCTGTGC AAAGAGGTGG CACCTTCCAG ATGGGGGGTA GCAGCAGCCA TAATAGGCCT
    TCTGGCAGTA ATGTGGACAC CCTCCTACGT CTTCGAGGAC GGCTCCTCCT GGATCACGAA
    GCCCTGTCTT GTCTCCTGGT CCTACTTTTT GTGGATGAGC CAAAACTCAA TACTAGCCGT
    CTTCACCGAG TACTGAGAAA TCTCTGCTAC CATGCCCAGA CCCGCCACTG GGTTATCCGT
    AGCCTGCTTT CCATCTTGCA GCGAAGCAGT GAGAGTGAGC TTTGCATTGA AACACCCAAG
    CTCTCCTCAA GTGAGGAAAA GGGCAAAAAG TCAAGCAAGA GCTGTGGGTC AAGCAGCCAT
    GAGAACCGGC CCCTGGACCT GCTACACAAG ATGGAGTCTA AGAGTTCCAA CCAGCTTTCC
    TGGCTCTCAG TATCCATGGA TGCAGCCCTA GGCTGCAGGA CTAATATATT CCAGATCCAG
    CGTTCAGGGG GTCGCAAACA TACTGAGAAA CATGCGAGTA GTGGCTCCAC TGTCCATATC
    CACCCCCAGG CTGCTCCTGT TGTCTGCAGA CATGTTCTGG ATACACTCAT TCAATTGGCC
    AAGGTGTTTC CCAGTCACTT CACACAGCAG CGGACCAAAG AAACAAACTG TGAGAGTGAT
    CGGGAGAGGG GCAGTAAGGC CTGCAGCCCA TGCTCTTCAC AGTCCACAAG CAGTGGCATT
    TGCACAGACT TCTGGGACTT ATTGGTAAAA CTGGACAACA TGAATGTCAG CCGGAAAGGC
    AAGAACTCCG TGAAGTCAGT GCCAGTGAGC GCTGGTGGTG AGGGGGAAAC CTCCCCATAC
    AGCCTCGAAG CTTCTCCACT GGGGCAACTC ATGAACATGT TGTCACACCC AGTCATCCGC
    CGGAGCTCTC TCTTAACTGA GAAGCTCCTC AGACTTCTTT CTCTCATCTC AATTGCTCTC
    CCAGAAAACA AAGTGTCGGA AGCACAGGCT AATTCTGGCA GCAGTGCTTC CTCCACCACT
    GTTGCCACTT CAACCACATC TACCACCACT ATCAGTGCCA CCTCCAGCAC ATCTACTCTG
    CCTGCTGCAA CCACCCCTGT CACTTCTGTT CCAGCCCTGG TTGCTGCCAC GGCTATATCC
    ACCATTGCCG TAGCCGCTTC AACCACAGTG ACTACCCCCA CAACTGCTAC CACTACTGTT
    TCAACTGCTA CTACTACTAA GGCCAGCAAA TCTCCAGCTA AAGTTGGTGA TATCGGCAGC
    AGCAGCAGCA CAGACTTTAA GATGGTATCC TCTGGCCTCA CTGAAAATCA GCTCCAGCTC
    TCTGTGGAAG TGTTGACATC CCATTCTTGT TCTGAAGAAG GCCTAGAAGA TGCAGCAAAT
    GTGTTACTGC AGCTCTCTCG GGGGGACCCT GGGACCCGGG ACACTGTTTT GAAGTTGCTA
    CTGAATGGAG CCCGCCATCT GGGTTATACG CTTTGTAAAC AGATAGGAAC TCTTCTGGCT
    GAGCTACGGG AATACAATCT GGAACAGCAG CGGCGAGCCC AGTGTGAAAC CCTCTCCCCT
    GATGGCATGC CTGAGGAACA GCCACAGACC ACCAAGCTGA AAGGCAAAAT GCAGAGCAGG
    TTTGACGTGG CTGAGAATGT GGTAATCGTG GCATCTCAGA AGCGGCCTCT GGGTGGCCGG
    GAGCTCCAGC TGCCTTCTAT GTCCATGTTG ACATCCAAGA CATCTACCCA GAAGTTCTTC
    TTGAGGGTAC TGCAGGTCAT CATCCAGCTC CGGGATGACA CGCGCCGTGC TAACAAGAAG
    GCCAAGCAGA CAGGCAGGCT AGGTTCCTCC GGTTTAGGCT CAGCTAGCAG CATCCAGGCA
    GCTGTTCGGC AGCTGGAGGC TGAGGCTGAT GCCATTATAC AAATGGTACG TGAGGGTCAG
    AGGGCGCGGA GACAGCAACA AGCAGCAACG TCGGAGTCAA GCCAGTCAGA GGCATCTGTC
    CGAAGGGAGG AGTCACCCAT GGATGTGGAC CAGCCATCTC CCAGTGCTCA AGATACTCAG
    TCCATTGGCT CAGATGGAAC TCCACAGGGA GAGAAGGAGA AGGAGGAGAG GCCACCTGAG
    TTACCCCTGC TCAGTGAGCA GCTGAACTTG GATGAGCTGT GGGACATGCT GGGAGAGTGT
    CTAAAGGAAC TGGAGGAATC CCATGACCAG CATGCGGTGC TAGTGCTACA ACCTGCTGTC
    GAGGCCTTCT TTCTGGTCCA TGCAACAGAG CGGGAGAGCA AGCCTCCTGT CCGGGACACC
    CGTGAGAGCC AGCTGGCACA CATCAAGGAC GAGCCACCTC CACTCTCCCC TGCCCCTTTA
    ACCCCAGCCA CTCCTTCCTC CCTTGACCCG TTCTTCTCCC GGGAGCCCTC ATCTATGCAC
    ATCTCCTCAA GCCTGCCCCC TGACACACAG AAGTTTCTTC GTTTTGCAGA GACTCATCGC
    ACTGTGTTAA ACCAGATCCT ACGGCAGTCC ACCACCCACC TCGCTGATGG GCCTTTTGCT
    GTCCTGGTAG ACTACATTCG TGTCCTCGAC TTCGATGTCA AGCGCAAATA TTTCCGCCAA
    GAGCTGGAGC GTTTGGACGA GGGACTCCGG AAAGAAGACA TGGCTGTGCA CGTTCGTCGT
    GATCATGTCT TTGAAGACTC CTATCGTGAG CTGCACCGGA AATCCCCTGA AGAAATGAAG
    AATCGATTGT ATATAGTATT TGAAGGAGAA GAAGGGCAGG ATGCTGGAGG GCTCCTGCGG
    GAGTGGTATA TGATCATCTC TCGAGAGATG TTTAACCCTA TGTATGCCTT GTTCCGTACC
    TCACCTGGTG ATCGGGTCAC ATACACCATC AATCCATCTT CTCACTGCAA CCCCAACCAC
    CTCAGCTACT TCAAGTTTGT TGGACGCATC GTGGCCAAAG CTGTGTATGA CAACCGCCTC
    CTGGAGTGCT ACTTTACTAG GTCCTTCTAC AAACACATTT TGGGCAAGTC TGTCAGATAT
    ACAGATATGG AGAGTGAAGA CTACCACTTT TACCAGGGCC TGGTTTATCT GCTGGAAAAT
    GATGTCTCCA CACTAGGCTA TGATCTCACT TTCAGCACTG AGGTCCAAGA GTTTGGAGTC
    TGTGAAGTTC GTGACCTCAA ACCCAATGGG GCCAACATCT TGGTAACAGA GGAAAATAAG
    AAGGAATATG TACACCTGGT GTGCCAGATG AGAATGACAG GAGCCATCCG CAAACAGCTG
    GCGGCTTTCT TAGAAGGTTT CTATGAGATC ATTCCAAAGC GCCTCATTTC CATCTTCACT
    GAGCAGGAGT TAGAGCTGCT CATATCAGGA CTGCCCACAA TCGACATTGA CGATTTGAAA
    TCTAACACTG AATATCACAA GTACCAGTCC AACTCTATTC AGATCCAGTG GTTCTGGAGA
    GCACTGCGTT CTTTCGATCA AGCTGACCGT GCCAAGTTCC TCCAGTTTGT CACAGGTACT
    TCCAAGGTGC CCCTGCAAGG TTTTGCTGCC CTTGAAGGCA TGAATGGCAT TCAGAAGTTT
    CAAATCCATC GAGATGACAG GTCCACAGAC CGCCTGCCTT CAGCTCACAC ATGTTTTAAT
    CAGCTGGACC TGCCTGCCTA TGAGAGCTTT GAGAAGCTCC GCCACATGCT ACTATTGGCC
    ATCCAGGAGT GCTCTGAAGG CTTTGGGCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48123
    Clone ID Related Accession (Same CDS sequence) XM_024987898.1
    Accession Version XM_024987898.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    541
    601
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    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
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    2941
    3001
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    5881
    5941
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    6301
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    9301
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    9781
    9841
    9901
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    10021
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    10201
    10261
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    10381
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    10501
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    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGG ATCTGAGTAT GTCTGAAGAG
    GACCAGATGA TGAGAGCGAT TGCCATGTCT CTGGGACAGG ATATCCCAAT GGATCAAAGA
    GCAGAGTCAC CTGAGGAAGT TGCTTGCCGA AAGGAAGAGG AGGAACGGAA AGCTCGAGAA
    AAGCAGGAAG AGGAAGAGGC TAAATGTCTA GAGAAGTTCC AGGATGCTGA TCCATTGGAG
    CAAGATGAGC TCCACACTTT CACAGATACA ATGTTGCCAG GCTGCTTTCA CCTTCTTGAT
    GAGTTGCCAG ACACTGTATA CCGAGTGTGT GATCTGATCA TGACAGCAAT CAAACGAAAT
    GGAGCAGACT ATCGAGACAT GATTCTGAAG CAAGTAGTTA ATCAGGTGTG GGAAGCTGCT
    GATGTATTGA TCAAAGCTGC TCTTCCCCTG ACAACAAGTG ACACAAAAAC TGTATCAGAG
    TGGATCAGTC AGATGGCCAC TCTACCCCAG GCCTCTAATT TGGCCACTAG AATCTTGCTT
    TTAACACTGC TTTTTGAGGA ATTGAAGCTA CCTTGTGCTT GGGTGGTTGA ATCTAGTGGC
    ATCCTTAATG TCCTAATCAA ACTCTTGGAA GTGGTTCAGC CCTGCCTACA GGCCGCCAAG
    GAACAAAAGG AGGTCCAGAC CCCAAAGTGG ATCACTCCAG TGTTGCTCCT GATTGATTTC
    TATGAAAAAA CAGCCATTTC CTCAAAGAGG AGAGCCCAAA TGACTAAGTA TCTACAGTCT
    AACAACAACA ACTGGCGCTG GTTTGATGAC CGCTCTGGAC GTTGGTGTAG TTACAGTGCA
    AGCAACAACA GCACTATTGA TTCTGCCTGG AAATCTGGAG AGACAAGTGT GCGTTTCACT
    GCAGGCCGAA GAAGATACAC TGTCCAGTTC ACTACAATGG TACAGGTTAA TGAGGAAACA
    GGGAACCGGC GTCCTGTGAT GCTGACACTC CTGAGGGTAC CCCGATTGAA TAAAAATTCT
    AAAAACAGCA ATGGACAGGA ACTAGAGAAG ACACTGGAAG AAAGCAAAGA AATGGATATC
    AAACGTAAAG AAAATAAAAC CAGTGATACC CCCTTGGCCC TAGACAGTAC AAATACTGAA
    AAGGAGACAA GCCTGGAAGA AACAAAAATT GGGGAGATCT TGATCCAGGG CCTGACAGAG
    GATATGGTGA CTGTTTTAAT CCGGGCCTGT GTGAGCATGC TAGGCGTCCC TGTGGATCCA
    GACACTTTGC ATGCCACCCT TCGCCTATGC CTGAGGCTCA CCCGAGACCA CAAATATGCC
    ATGATGTTTG CAGAGCTGAA GAGTACTCGC ATGATCTTAA ATTTGACCCA GAGTTCAGGC
    TTCAATGGGT TTACTCCCCT GGTTACCCTT CTCTTAAGAC ACATCATTGA GGACCCCTGC
    ACCCTGCGCC ATACCATGGA AAAGGTTGTT CGCTCAGCAG CTACAAGTGG AGCTGGTAGC
    ACTACTTCTG GTGTTGTATC TGGCAGCCTT GGCTCTCGGG AGATCAACTA CATCCTTCGT
    GTCCTTGGAC CAGCCGCATG CCGCAACCCA GACATTTTCA CAGAAGTGGC CAACTGCTGT
    ATCCGCATTG CCCTTCCAGC CCCTCGCGGC TCAGGAACTG CTTCAGATGA TGAGTTTGAG
    AATCTCAGAA TTAAAGGCCC GAATGCTGTA CAGCTGGTGA AAACAACCCC TTTGAAGCCC
    TCACCTCTGC CTGTCATTCC TGATACTATC AAGGAAGTGA TCTATGACAT GCTAAATGCC
    CTGGCTGCAT ACCATGCTCC AGAGGAAGCA GACAAATCTG ATCCTAAACC AGGGGGTATG
    ACTCAAGAGG TTGGCCAGCT CCTGCAAGAC ATGGGTGATG ACGTATACCA GCAGTACCGG
    TCACTTACCC GTCAGAGCAG TGACTTTGAT GCACAGACAG GATTTTCCAT TAATAGTCAG
    GTCTTTGCTG CAGATGGTTC CTCCACTGAG ACTTCCGCAT CTGGGGTATC CCAAGGAGAA
    GTGTCAACTC CGGAGGAGTC TCGGGATGGG AAGAAAGATA AAGAAGGGGA CCGGGCCTCT
    GAGGAAGGCA AGCAGAAAGG CAAGGGCAGC AAACCTTTAA TGCCCACCTC CACTATTCTT
    CGGCTTCTGG CAGAGTTGGT GAGATCCTAT GTTGGTATTG CTACCCTGAT TGCCAACTAC
    AGCTACACTG TGGGCCAGTC TGAGCTGATC AAAGAGGACT GCAGTGTGCT AGCTTTTGTC
    TTGGACCACC TCCTCCCGCA TACCCAGAAT GCAGAAGACA AAGACACACC GGCGCTGGCC
    CGCCTGTTCC TCGCAAGCCT GGCTGCCTCA GGGAGTGGCA CAGATGCCCA GGTGGCCCTT
    GTGAATGAAG TAAAAGCAGC CCTGGGAAGG GCACTGGCTA TGGCTGAGAG TACAGAGAAA
    CATGCCAGAC TTCAGGCAGT GATGTGTATC ATCAGTACTA TCATGGAGTC CTGCCCCTCC
    ACCTCCAGTT TCTACAGCAG TGCAACAGCC AAGACCCAAC ACAACGGCAT GAACAACATC
    ATTCGACTCT TCCTGAAGAA GGGACTGGTT AATGACCTGG CCAGAGTGCC TCACAGCCTG
    GACCTGTCCA GTCCCAACAT GGCCAACACT GTCAATGCTG CTCTGAAGCC TTTGGAAACA
    CTTTCCCGGA TTGTGAACCA GCCCAGTAGC CTTTTTGGCA GTAAGAGTGC TTCTAGCAAG
    AGCAAGTCTG AGCAGGATGC CCAAGGAGCT GCTCAGGACT CTAATAGCAA CCAACAGGAC
    CCAGGCGAGC CTGGGGAAAC AGAAGTGCAG GAGGAGGATC ATGATGTCAC TCAGACAGAG
    GTGGCAGATG GGGATATCAT GGATGGGGAG GCTGAAACCG ACTCAGTGGT GATTGCTGGG
    CAGCCTGAGG TGCTCAGTTC ACAAGAGATG CAGGTTGAGA ATGAGCTGGA GGACCTGATA
    GATGAGTTGC TTGAGAGGGA TGGCGGATCT GGGAACAGTA CAATTATAGT GAGCAGAAGT
    GGAGAGGATG AATCACAAGA GGACGTGCTG ATGGATGAAG CTCCTTCCAA CCTCAGCCAA
    GCTTCCACCT TGCAGGCCAA CCGAGAAGAT TCCATGAATA TCCTGGACCC TGAGGATGAG
    GAGGAGCACA CTCAGGAAGA GGACAGCAGT GGCAGTAACG AGGATGAGGA TGATAGTCAG
    GATGAAGAGG AGGAGGAGGA GGAAGATGAG GAAGATGATC AGGAGGATGA TGAAGGTGAA
    GAGGGAGATG AAGACGATGA CGACGATGGC TCTGAAATGG AATTGGATGA GGATTACCCT
    GATATGAACG CTTCTCCCTT GGTCCGATTT GAGCGCTTTG ACCGGGAGGA TGATCTCATC
    ATTGAGTTTG ACAACATGTT CTCCAGTGCT ACAGACATCC CCCCATCCCC AGGAAATATC
    CCTACCACCC ACCCACTGAT GGTACGCCAT GCAGATCACA GTTCCCTGAC ACTGGGCAGT
    GGCTCCTCTA CAACACGTCT CACCCAGGGC ATTGGGCGCA GTCAAAGGAC CCTGAGACAG
    CTGACAGCCA ACACAGGCCA TACCATTCAC GTTCACTACC CTGGGAATCG CCAGCCCAAT
    CCTCCTCTTA TACTCCAGAG GTTGCTTGGT CCCTCTGCTG CTGCTGACAT CCTTCAGCTG
    AGCAGCAGCC TTCCCCTACA AAGCCGAGGC CGAGCCCGCC TCCTAGTGGG CAACGATGAC
    GTCCACATCA TCGCCCGGTC TGATGATGAA CTACTGGATG ACTTTTTCCA TGATCAGAGC
    ACAGCTACCA GCCAAGCAGG GACCCTATCC AGTATCCCTA CAGCCCTGAC TCGCTGGACA
    GAGGAATGCA AAGTTCTTGA TGCTGAGAGC ATGCATGACT GTGTTTCAGT TGTTAAAGTG
    CCCATTGTCA ATCATCTGGA ATTCCTGAGG GATGAGGAGC TGGAAGAAAG GAGGGAGAAA
    AGGAGGAAAC AACTAGCTGA GGAAGAAACA AAGATAACCG ACAAAGGCAA AGAAGATAAG
    GAGAACAGGG ATCAGAGTGC ACAGTGTGCT GCATCTAAGA CAAATGACTC CACTGAACAG
    AACCTCTCAG ATGGGACTCC TATGCCTGAC AGCTACCCAG CAACCCCATC TTCAACTGAT
    GTAGCTACAT CTGAGCCCAG GGAGACCCTT GTCACTCTGC AGCCCTCCCA ACAGCAACCA
    ACACTCCCAA CCCCACCAGC CTTGGGAGAG ATTCCTCAAG AGCTGCAGTC CCCAATTGGA
    GAAGGTGGGA GCTCTACACA GCTATTGATG CCTGTAGAGC CAGAGGAATT GGGTCCCACA
    AGGCCAAGTG GAGAAGCAGA AACAACTCAG ATGGAGCTAT CTCCAGCTCC CACTATAACC
    TCTCTTTCCC CAGAGAGAGC TGAAGATTCT GATGCTCTGA CAGCTGTCAG CAGTCAACTG
    GAAGGCTCTC CCATGGATAC TAGCAGCCTG GCTTCCTGTA CCCTGGAGGA GGCTGTGGGT
    GACGCCCCAG CAACTGGCAG TTCTGAGCAG CCCACAGCAG GCAGCTCCAC TCCTGGGGAT
    GCCCCACCAG TTGTGACAGA AGTACAAGGC AGGGGTGATG GGTCAGGGGA ACCTACCCAG
    CCCCCTGAGG ACAGCTCTCC CCCTGCATCC TCTGAGAGTT CTTCCACCAG AGATTCCGCG
    GTGGCCATTT CTGGAGCAGA TTCCCGGGGA ATCCTGGAAG AGCCATTGCC TTCAACAAGC
    AGTGAAGAAG AAGATCCTCT TGCAGGTATT AGTCTCCCCG AAGGTGTGGA CCCCTCTTTT
    CTGGCCGCTC TGCCTGATGA CATTCGCCGA GAAGTCCTAC AGAACCAGCT GGGCATTCGT
    CCACCAACAC GAACTGCCCC TTCAACAAAT AACTCAGCTC CTGCAGTGGT GGGGAATCCT
    GGTGTCACCG AAGTGAGCCC TGAGTTCCTG GCTGCTCTGC CTCCAGCCAT CCAGGAGGAA
    GTACTGGCAC AGCAGAGAGC TGAGCAACAG CGACGGGAAC TAGCACAAAA TGCCAGCTCA
    GACACCCCCA TGGACCCTGT GACCTTCATC CAGACTCTGC CCTCAGACCT GCGCCGTAGT
    GTCCTAGAGG ACATGGAGGA CAGTGTGTTG GCCGTGATGC CACCTGACAT CGCAGCTGAA
    GCTCAAGCCC TGAGGCGAGA GCAAGAAGCC CGGCAGAGGC AGCTCATGCA TGAGCGACTT
    TTTGGGCACA GCAGCACCTC TGCACTCTCT GCCATTCTCC GAAGCCCAGC CTTCACCAGT
    CGCCTGAGTG GCAACCGTGG GGTCCAATAC ACTCGCCTTG CTGTGCAAAG AGGTGGCACC
    TTCCAGATGG GGGGTAGCAG CAGCCATAAT AGGCCTTCTG GCAGTAATGT GGACACCCTC
    CTACGTCTTC GAGGACGGCT CCTCCTGGAT CACGAAGCCC TGTCTTGTCT CCTGGTCCTA
    CTTTTTGTGG ATGAGCCAAA ACTCAATACT AGCCGTCTTC ACCGAGTACT GAGAAATCTC
    TGCTACCATG CCCAGACCCG CCACTGGGTT ATCCGTAGCC TGCTTTCCAT CTTGCAGCGA
    AGCAGTGAGA GTGAGCTTTG CATTGAAACA CCCAAGCTCT CCTCAAGTGA GGAAAAGGGC
    AAAAAGTCAA GCAAGAGCTG TGGGTCAAGC AGCCATGAGA ACCGGCCCCT GGACCTGCTA
    CACAAGATGG AGTCTAAGAG TTCCAACCAG CTTTCCTGGC TCTCAGTATC CATGGATGCA
    GCCCTAGGCT GCAGGACTAA TATATTCCAG ATCCAGCGTT CAGGGGGTCG CAAACATACT
    GAGAAACATG CGAGTAGTGG CTCCACTGTC CATATCCACC CCCAGGCTGC TCCTGTTGTC
    TGCAGACATG TTCTGGATAC ACTCATTCAA TTGGCCAAGG TGTTTCCCAG TCACTTCACA
    CAGCAGCGGA CCAAAGAAAC AAACTGTGAG AGTGATCGGG AGAGGGGCAG TAAGGCCTGC
    AGCCCATGCT CTTCACAGTC CACAAGCAGT GGCATTTGCA CAGACTTCTG GGACTTATTG
    GTAAAACTGG ACAACATGAA TGTCAGCCGG AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA
    GTGAGCGCTG GTGGTGAGGG GGAAACCTCC CCATACAGCC TCGAAGCTTC TCCACTGGGG
    CAACTCATGA ACATGTTGTC ACACCCAGTC ATCCGCCGGA GCTCTCTCTT AACTGAGAAG
    CTCCTCAGAC TTCTTTCTCT CATCTCAATT GCTCTCCCAG AAAACAAAGT GTCGGAAGCA
    CAGGCTAATT CTGGCAGCAG TGCTTCCTCC ACCACTGTTG CCACTTCAAC CACATCTACC
    ACCACTATCA GTGCCACCTC CAGCACATCT ACTCTGCCTG CTGCAACCAC CCCTGTCACT
    TCTGTTCCAG CCCTGGTTGC TGCCACGGCT ATATCCACCA TTGCCGTAGC CGCTTCAACC
    ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTGCTACTAC TACTAAGGCC
    AGCAAATCTC CAGCTAAAGT TGGTGATATC GGCAGCAGCA GCAGCACAGA CTTTAAGATG
    GTATCCTCTG GCCTCACTGA AAATCAGCTC CAGCTCTCTG TGGAAGTGTT GACATCCCAT
    TCTTGTTCTG AAGAAGGCCT AGAAGATGCA GCAAATGTGT TACTGCAGCT CTCTCGGGGG
    GACCCTGGGA CCCGGGACAC TGTTTTGAAG TTGCTACTGA ATGGAGCCCG CCATCTGGGT
    TATACGCTTT GTAAACAGAT AGGAACTCTT CTGGCTGAGC TACGGGAATA CAATCTGGAA
    CAGCAGCGGC GAGCCCAGTG TGAAACCCTC TCCCCTGATG GCATGCCTGA GGAACAGCCA
    CAGACCACCA AGCTGAAAGG CAAAATGCAG AGCAGGTTTG ACGTGGCTGA GAATGTGGTA
    ATCGTGGCAT CTCAGAAGCG GCCTCTGGGT GGCCGGGAGC TCCAGCTGCC TTCTATGTCC
    ATGTTGACAT CCAAGACATC TACCCAGAAG TTCTTCTTGA GGGTACTGCA GGTCATCATC
    CAGCTCCGGG ATGACACGCG CCGTGCTAAC AAGAAGGCCA AGCAGACAGG CAGGCTAGGT
    TCCTCCGGTT TAGGCTCAGC TAGCAGCATC CAGGCAGCTG TTCGGCAGCT GGAGGCTGAG
    GCTGATGCCA TTATACAAAT GGTACGTGAG GGTCAGAGGG CGCGGAGACA GCAACAAGCA
    GCAACGTCGG AGTCAAGCCA GTCAGAGGCA TCTGTCCGAA GGGAGGAGTC ACCCATGGAT
    GTGGACCAGC CATCTCCCAG TGCTCAAGAT ACTCAGTCCA TTGGCTCAGA TGGAACTCCA
    CAGGGAGAGA AGGAGAAGGA GGAGAGGCCA CCTGAGTTAC CCCTGCTCAG TGAGCAGCTG
    AACTTGGATG AGCTGTGGGA CATGCTGGGA GAGTGTCTAA AGGAACTGGA GGAATCCCAT
    GACCAGCATG CGGTGCTAGT GCTACAACCT GCTGTCGAGG CCTTCTTTCT GGTCCATGCA
    ACAGAGCGGG AGAGCAAGCC TCCTGTCCGG GACACCCGTG AGAGCCAGCT GGCACACATC
    AAGGACGAGC CACCTCCACT CTCCCCTGCC CCTTTAACCC CAGCCACTCC TTCCTCCCTT
    GACCCGTTCT TCTCCCGGGA GCCCTCATCT ATGCACATCT CCTCAAGCCT GCCCCCTGAC
    ACACAGAAGT TTCTTCGTTT TGCAGAGACT CATCGCACTG TGTTAAACCA GATCCTACGG
    CAGTCCACCA CCCACCTCGC TGATGGGCCT TTTGCTGTCC TGGTAGACTA CATTCGTGTC
    CTCGACTTCG ATGTCAAGCG CAAATATTTC CGCCAAGAGC TGGAGCGTTT GGACGAGGGA
    CTCCGGAAAG AAGACATGGC TGTGCACGTT CGTCGTGATC ATGTCTTTGA AGACTCCTAT
    CGTGAGCTGC ACCGGAAATC CCCTGAAGAA ATGAAGAATC GATTGTATAT AGTATTTGAA
    GGAGAAGAAG GGCAGGATGC TGGAGGGCTC CTGCGGGAGT GGTATATGAT CATCTCTCGA
    GAGATGTTTA ACCCTATGTA TGCCTTGTTC CGTACCTCAC CTGGTGATCG GGTCACATAC
    ACCATCAATC CATCTTCTCA CTGCAACCCC AACCACCTCA GCTACTTCAA GTTTGTTGGA
    CGCATCGTGG CCAAAGCTGT GTATGACAAC CGCCTCCTGG AGTGCTACTT TACTAGGTCC
    TTCTACAAAC ACATTTTGGG CAAGTCTGTC AGATATACAG ATATGGAGAG TGAAGACTAC
    CACTTTTACC AGGGCCTGGT TTATCTGCTG GAAAATGATG TCTCCACACT AGGCTATGAT
    CTCACTTTCA GCACTGAGGT CCAAGAGTTT GGAGTCTGTG AAGTTCGTGA CCTCAAACCC
    AATGGGGCCA ACATCTTGGT AACAGAGGAA AATAAGAAGG AATATGTACA CCTGGTGTGC
    CAGATGAGAA TGACAGGAGC CATCCGCAAA CAGCTGGCGG CTTTCTTAGA AGGTTTCTAT
    GAGATCATTC CAAAGCGCCT CATTTCCATC TTCACTGAGC AGGAGTTAGA GCTGCTCATA
    TCAGGACTGC CCACAATCGA CATTGACGAT TTGAAATCTA ACACTGAATA TCACAAGTAC
    CAGTCCAACT CTATTCAGAT CCAGTGGTTC TGGAGAGCAC TGCGTTCTTT CGATCAAGCT
    GACCGTGCCA AGTTCCTCCA GTTTGTCACA GGTACTTCCA AGGTGCCCCT GCAAGGTTTT
    GCTGCCCTTG AAGGCATGAA TGGCATTCAG AAGTTTCAAA TCCATCGAGA TGACAGGTCC
    ACAGACCGCC TGCCTTCAGC TCACACATGT TTTAATCAGC TGGACCTGCC TGCCTATGAG
    AGCTTTGAGA AGCTCCGCCA CATGCTACTA TTGGCCATCC AGGAGTGCTC TGAAGGCTTT
    GGGCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843666.1
    CDS442..13593
    Translation

    Target ORF information:

    RefSeq Version XM_024987898.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987898.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGG ATCTGAGTAT GTCTGAAGAG
    GACCAGATGA TGAGAGCGAT TGCCATGTCT CTGGGACAGG ATATCCCAAT GGATCAAAGA
    GCAGAGTCAC CTGAGGAAGT TGCTTGCCGA AAGGAAGAGG AGGAACGGAA AGCTCGAGAA
    AAGCAGGAAG AGGAAGAGGC TAAATGTCTA GAGAAGTTCC AGGATGCTGA TCCATTGGAG
    CAAGATGAGC TCCACACTTT CACAGATACA ATGTTGCCAG GCTGCTTTCA CCTTCTTGAT
    GAGTTGCCAG ACACTGTATA CCGAGTGTGT GATCTGATCA TGACAGCAAT CAAACGAAAT
    GGAGCAGACT ATCGAGACAT GATTCTGAAG CAAGTAGTTA ATCAGGTGTG GGAAGCTGCT
    GATGTATTGA TCAAAGCTGC TCTTCCCCTG ACAACAAGTG ACACAAAAAC TGTATCAGAG
    TGGATCAGTC AGATGGCCAC TCTACCCCAG GCCTCTAATT TGGCCACTAG AATCTTGCTT
    TTAACACTGC TTTTTGAGGA ATTGAAGCTA CCTTGTGCTT GGGTGGTTGA ATCTAGTGGC
    ATCCTTAATG TCCTAATCAA ACTCTTGGAA GTGGTTCAGC CCTGCCTACA GGCCGCCAAG
    GAACAAAAGG AGGTCCAGAC CCCAAAGTGG ATCACTCCAG TGTTGCTCCT GATTGATTTC
    TATGAAAAAA CAGCCATTTC CTCAAAGAGG AGAGCCCAAA TGACTAAGTA TCTACAGTCT
    AACAACAACA ACTGGCGCTG GTTTGATGAC CGCTCTGGAC GTTGGTGTAG TTACAGTGCA
    AGCAACAACA GCACTATTGA TTCTGCCTGG AAATCTGGAG AGACAAGTGT GCGTTTCACT
    GCAGGCCGAA GAAGATACAC TGTCCAGTTC ACTACAATGG TACAGGTTAA TGAGGAAACA
    GGGAACCGGC GTCCTGTGAT GCTGACACTC CTGAGGGTAC CCCGATTGAA TAAAAATTCT
    AAAAACAGCA ATGGACAGGA ACTAGAGAAG ACACTGGAAG AAAGCAAAGA AATGGATATC
    AAACGTAAAG AAAATAAAAC CAGTGATACC CCCTTGGCCC TAGACAGTAC AAATACTGAA
    AAGGAGACAA GCCTGGAAGA AACAAAAATT GGGGAGATCT TGATCCAGGG CCTGACAGAG
    GATATGGTGA CTGTTTTAAT CCGGGCCTGT GTGAGCATGC TAGGCGTCCC TGTGGATCCA
    GACACTTTGC ATGCCACCCT TCGCCTATGC CTGAGGCTCA CCCGAGACCA CAAATATGCC
    ATGATGTTTG CAGAGCTGAA GAGTACTCGC ATGATCTTAA ATTTGACCCA GAGTTCAGGC
    TTCAATGGGT TTACTCCCCT GGTTACCCTT CTCTTAAGAC ACATCATTGA GGACCCCTGC
    ACCCTGCGCC ATACCATGGA AAAGGTTGTT CGCTCAGCAG CTACAAGTGG AGCTGGTAGC
    ACTACTTCTG GTGTTGTATC TGGCAGCCTT GGCTCTCGGG AGATCAACTA CATCCTTCGT
    GTCCTTGGAC CAGCCGCATG CCGCAACCCA GACATTTTCA CAGAAGTGGC CAACTGCTGT
    ATCCGCATTG CCCTTCCAGC CCCTCGCGGC TCAGGAACTG CTTCAGATGA TGAGTTTGAG
    AATCTCAGAA TTAAAGGCCC GAATGCTGTA CAGCTGGTGA AAACAACCCC TTTGAAGCCC
    TCACCTCTGC CTGTCATTCC TGATACTATC AAGGAAGTGA TCTATGACAT GCTAAATGCC
    CTGGCTGCAT ACCATGCTCC AGAGGAAGCA GACAAATCTG ATCCTAAACC AGGGGGTATG
    ACTCAAGAGG TTGGCCAGCT CCTGCAAGAC ATGGGTGATG ACGTATACCA GCAGTACCGG
    TCACTTACCC GTCAGAGCAG TGACTTTGAT GCACAGACAG GATTTTCCAT TAATAGTCAG
    GTCTTTGCTG CAGATGGTTC CTCCACTGAG ACTTCCGCAT CTGGGGTATC CCAAGGAGAA
    GTGTCAACTC CGGAGGAGTC TCGGGATGGG AAGAAAGATA AAGAAGGGGA CCGGGCCTCT
    GAGGAAGGCA AGCAGAAAGG CAAGGGCAGC AAACCTTTAA TGCCCACCTC CACTATTCTT
    CGGCTTCTGG CAGAGTTGGT GAGATCCTAT GTTGGTATTG CTACCCTGAT TGCCAACTAC
    AGCTACACTG TGGGCCAGTC TGAGCTGATC AAAGAGGACT GCAGTGTGCT AGCTTTTGTC
    TTGGACCACC TCCTCCCGCA TACCCAGAAT GCAGAAGACA AAGACACACC GGCGCTGGCC
    CGCCTGTTCC TCGCAAGCCT GGCTGCCTCA GGGAGTGGCA CAGATGCCCA GGTGGCCCTT
    GTGAATGAAG TAAAAGCAGC CCTGGGAAGG GCACTGGCTA TGGCTGAGAG TACAGAGAAA
    CATGCCAGAC TTCAGGCAGT GATGTGTATC ATCAGTACTA TCATGGAGTC CTGCCCCTCC
    ACCTCCAGTT TCTACAGCAG TGCAACAGCC AAGACCCAAC ACAACGGCAT GAACAACATC
    ATTCGACTCT TCCTGAAGAA GGGACTGGTT AATGACCTGG CCAGAGTGCC TCACAGCCTG
    GACCTGTCCA GTCCCAACAT GGCCAACACT GTCAATGCTG CTCTGAAGCC TTTGGAAACA
    CTTTCCCGGA TTGTGAACCA GCCCAGTAGC CTTTTTGGCA GTAAGAGTGC TTCTAGCAAG
    AGCAAGTCTG AGCAGGATGC CCAAGGAGCT GCTCAGGACT CTAATAGCAA CCAACAGGAC
    CCAGGCGAGC CTGGGGAAAC AGAAGTGCAG GAGGAGGATC ATGATGTCAC TCAGACAGAG
    GTGGCAGATG GGGATATCAT GGATGGGGAG GCTGAAACCG ACTCAGTGGT GATTGCTGGG
    CAGCCTGAGG TGCTCAGTTC ACAAGAGATG CAGGTTGAGA ATGAGCTGGA GGACCTGATA
    GATGAGTTGC TTGAGAGGGA TGGCGGATCT GGGAACAGTA CAATTATAGT GAGCAGAAGT
    GGAGAGGATG AATCACAAGA GGACGTGCTG ATGGATGAAG CTCCTTCCAA CCTCAGCCAA
    GCTTCCACCT TGCAGGCCAA CCGAGAAGAT TCCATGAATA TCCTGGACCC TGAGGATGAG
    GAGGAGCACA CTCAGGAAGA GGACAGCAGT GGCAGTAACG AGGATGAGGA TGATAGTCAG
    GATGAAGAGG AGGAGGAGGA GGAAGATGAG GAAGATGATC AGGAGGATGA TGAAGGTGAA
    GAGGGAGATG AAGACGATGA CGACGATGGC TCTGAAATGG AATTGGATGA GGATTACCCT
    GATATGAACG CTTCTCCCTT GGTCCGATTT GAGCGCTTTG ACCGGGAGGA TGATCTCATC
    ATTGAGTTTG ACAACATGTT CTCCAGTGCT ACAGACATCC CCCCATCCCC AGGAAATATC
    CCTACCACCC ACCCACTGAT GGTACGCCAT GCAGATCACA GTTCCCTGAC ACTGGGCAGT
    GGCTCCTCTA CAACACGTCT CACCCAGGGC ATTGGGCGCA GTCAAAGGAC CCTGAGACAG
    CTGACAGCCA ACACAGGCCA TACCATTCAC GTTCACTACC CTGGGAATCG CCAGCCCAAT
    CCTCCTCTTA TACTCCAGAG GTTGCTTGGT CCCTCTGCTG CTGCTGACAT CCTTCAGCTG
    AGCAGCAGCC TTCCCCTACA AAGCCGAGGC CGAGCCCGCC TCCTAGTGGG CAACGATGAC
    GTCCACATCA TCGCCCGGTC TGATGATGAA CTACTGGATG ACTTTTTCCA TGATCAGAGC
    ACAGCTACCA GCCAAGCAGG GACCCTATCC AGTATCCCTA CAGCCCTGAC TCGCTGGACA
    GAGGAATGCA AAGTTCTTGA TGCTGAGAGC ATGCATGACT GTGTTTCAGT TGTTAAAGTG
    CCCATTGTCA ATCATCTGGA ATTCCTGAGG GATGAGGAGC TGGAAGAAAG GAGGGAGAAA
    AGGAGGAAAC AACTAGCTGA GGAAGAAACA AAGATAACCG ACAAAGGCAA AGAAGATAAG
    GAGAACAGGG ATCAGAGTGC ACAGTGTGCT GCATCTAAGA CAAATGACTC CACTGAACAG
    AACCTCTCAG ATGGGACTCC TATGCCTGAC AGCTACCCAG CAACCCCATC TTCAACTGAT
    GTAGCTACAT CTGAGCCCAG GGAGACCCTT GTCACTCTGC AGCCCTCCCA ACAGCAACCA
    ACACTCCCAA CCCCACCAGC CTTGGGAGAG ATTCCTCAAG AGCTGCAGTC CCCAATTGGA
    GAAGGTGGGA GCTCTACACA GCTATTGATG CCTGTAGAGC CAGAGGAATT GGGTCCCACA
    AGGCCAAGTG GAGAAGCAGA AACAACTCAG ATGGAGCTAT CTCCAGCTCC CACTATAACC
    TCTCTTTCCC CAGAGAGAGC TGAAGATTCT GATGCTCTGA CAGCTGTCAG CAGTCAACTG
    GAAGGCTCTC CCATGGATAC TAGCAGCCTG GCTTCCTGTA CCCTGGAGGA GGCTGTGGGT
    GACGCCCCAG CAACTGGCAG TTCTGAGCAG CCCACAGCAG GCAGCTCCAC TCCTGGGGAT
    GCCCCACCAG TTGTGACAGA AGTACAAGGC AGGGGTGATG GGTCAGGGGA ACCTACCCAG
    CCCCCTGAGG ACAGCTCTCC CCCTGCATCC TCTGAGAGTT CTTCCACCAG AGATTCCGCG
    GTGGCCATTT CTGGAGCAGA TTCCCGGGGA ATCCTGGAAG AGCCATTGCC TTCAACAAGC
    AGTGAAGAAG AAGATCCTCT TGCAGGTATT AGTCTCCCCG AAGGTGTGGA CCCCTCTTTT
    CTGGCCGCTC TGCCTGATGA CATTCGCCGA GAAGTCCTAC AGAACCAGCT GGGCATTCGT
    CCACCAACAC GAACTGCCCC TTCAACAAAT AACTCAGCTC CTGCAGTGGT GGGGAATCCT
    GGTGTCACCG AAGTGAGCCC TGAGTTCCTG GCTGCTCTGC CTCCAGCCAT CCAGGAGGAA
    GTACTGGCAC AGCAGAGAGC TGAGCAACAG CGACGGGAAC TAGCACAAAA TGCCAGCTCA
    GACACCCCCA TGGACCCTGT GACCTTCATC CAGACTCTGC CCTCAGACCT GCGCCGTAGT
    GTCCTAGAGG ACATGGAGGA CAGTGTGTTG GCCGTGATGC CACCTGACAT CGCAGCTGAA
    GCTCAAGCCC TGAGGCGAGA GCAAGAAGCC CGGCAGAGGC AGCTCATGCA TGAGCGACTT
    TTTGGGCACA GCAGCACCTC TGCACTCTCT GCCATTCTCC GAAGCCCAGC CTTCACCAGT
    CGCCTGAGTG GCAACCGTGG GGTCCAATAC ACTCGCCTTG CTGTGCAAAG AGGTGGCACC
    TTCCAGATGG GGGGTAGCAG CAGCCATAAT AGGCCTTCTG GCAGTAATGT GGACACCCTC
    CTACGTCTTC GAGGACGGCT CCTCCTGGAT CACGAAGCCC TGTCTTGTCT CCTGGTCCTA
    CTTTTTGTGG ATGAGCCAAA ACTCAATACT AGCCGTCTTC ACCGAGTACT GAGAAATCTC
    TGCTACCATG CCCAGACCCG CCACTGGGTT ATCCGTAGCC TGCTTTCCAT CTTGCAGCGA
    AGCAGTGAGA GTGAGCTTTG CATTGAAACA CCCAAGCTCT CCTCAAGTGA GGAAAAGGGC
    AAAAAGTCAA GCAAGAGCTG TGGGTCAAGC AGCCATGAGA ACCGGCCCCT GGACCTGCTA
    CACAAGATGG AGTCTAAGAG TTCCAACCAG CTTTCCTGGC TCTCAGTATC CATGGATGCA
    GCCCTAGGCT GCAGGACTAA TATATTCCAG ATCCAGCGTT CAGGGGGTCG CAAACATACT
    GAGAAACATG CGAGTAGTGG CTCCACTGTC CATATCCACC CCCAGGCTGC TCCTGTTGTC
    TGCAGACATG TTCTGGATAC ACTCATTCAA TTGGCCAAGG TGTTTCCCAG TCACTTCACA
    CAGCAGCGGA CCAAAGAAAC AAACTGTGAG AGTGATCGGG AGAGGGGCAG TAAGGCCTGC
    AGCCCATGCT CTTCACAGTC CACAAGCAGT GGCATTTGCA CAGACTTCTG GGACTTATTG
    GTAAAACTGG ACAACATGAA TGTCAGCCGG AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA
    GTGAGCGCTG GTGGTGAGGG GGAAACCTCC CCATACAGCC TCGAAGCTTC TCCACTGGGG
    CAACTCATGA ACATGTTGTC ACACCCAGTC ATCCGCCGGA GCTCTCTCTT AACTGAGAAG
    CTCCTCAGAC TTCTTTCTCT CATCTCAATT GCTCTCCCAG AAAACAAAGT GTCGGAAGCA
    CAGGCTAATT CTGGCAGCAG TGCTTCCTCC ACCACTGTTG CCACTTCAAC CACATCTACC
    ACCACTATCA GTGCCACCTC CAGCACATCT ACTCTGCCTG CTGCAACCAC CCCTGTCACT
    TCTGTTCCAG CCCTGGTTGC TGCCACGGCT ATATCCACCA TTGCCGTAGC CGCTTCAACC
    ACAGTGACTA CCCCCACAAC TGCTACCACT ACTGTTTCAA CTGCTACTAC TACTAAGGCC
    AGCAAATCTC CAGCTAAAGT TGGTGATATC GGCAGCAGCA GCAGCACAGA CTTTAAGATG
    GTATCCTCTG GCCTCACTGA AAATCAGCTC CAGCTCTCTG TGGAAGTGTT GACATCCCAT
    TCTTGTTCTG AAGAAGGCCT AGAAGATGCA GCAAATGTGT TACTGCAGCT CTCTCGGGGG
    GACCCTGGGA CCCGGGACAC TGTTTTGAAG TTGCTACTGA ATGGAGCCCG CCATCTGGGT
    TATACGCTTT GTAAACAGAT AGGAACTCTT CTGGCTGAGC TACGGGAATA CAATCTGGAA
    CAGCAGCGGC GAGCCCAGTG TGAAACCCTC TCCCCTGATG GCATGCCTGA GGAACAGCCA
    CAGACCACCA AGCTGAAAGG CAAAATGCAG AGCAGGTTTG ACGTGGCTGA GAATGTGGTA
    ATCGTGGCAT CTCAGAAGCG GCCTCTGGGT GGCCGGGAGC TCCAGCTGCC TTCTATGTCC
    ATGTTGACAT CCAAGACATC TACCCAGAAG TTCTTCTTGA GGGTACTGCA GGTCATCATC
    CAGCTCCGGG ATGACACGCG CCGTGCTAAC AAGAAGGCCA AGCAGACAGG CAGGCTAGGT
    TCCTCCGGTT TAGGCTCAGC TAGCAGCATC CAGGCAGCTG TTCGGCAGCT GGAGGCTGAG
    GCTGATGCCA TTATACAAAT GGTACGTGAG GGTCAGAGGG CGCGGAGACA GCAACAAGCA
    GCAACGTCGG AGTCAAGCCA GTCAGAGGCA TCTGTCCGAA GGGAGGAGTC ACCCATGGAT
    GTGGACCAGC CATCTCCCAG TGCTCAAGAT ACTCAGTCCA TTGGCTCAGA TGGAACTCCA
    CAGGGAGAGA AGGAGAAGGA GGAGAGGCCA CCTGAGTTAC CCCTGCTCAG TGAGCAGCTG
    AACTTGGATG AGCTGTGGGA CATGCTGGGA GAGTGTCTAA AGGAACTGGA GGAATCCCAT
    GACCAGCATG CGGTGCTAGT GCTACAACCT GCTGTCGAGG CCTTCTTTCT GGTCCATGCA
    ACAGAGCGGG AGAGCAAGCC TCCTGTCCGG GACACCCGTG AGAGCCAGCT GGCACACATC
    AAGGACGAGC CACCTCCACT CTCCCCTGCC CCTTTAACCC CAGCCACTCC TTCCTCCCTT
    GACCCGTTCT TCTCCCGGGA GCCCTCATCT ATGCACATCT CCTCAAGCCT GCCCCCTGAC
    ACACAGAAGT TTCTTCGTTT TGCAGAGACT CATCGCACTG TGTTAAACCA GATCCTACGG
    CAGTCCACCA CCCACCTCGC TGATGGGCCT TTTGCTGTCC TGGTAGACTA CATTCGTGTC
    CTCGACTTCG ATGTCAAGCG CAAATATTTC CGCCAAGAGC TGGAGCGTTT GGACGAGGGA
    CTCCGGAAAG AAGACATGGC TGTGCACGTT CGTCGTGATC ATGTCTTTGA AGACTCCTAT
    CGTGAGCTGC ACCGGAAATC CCCTGAAGAA ATGAAGAATC GATTGTATAT AGTATTTGAA
    GGAGAAGAAG GGCAGGATGC TGGAGGGCTC CTGCGGGAGT GGTATATGAT CATCTCTCGA
    GAGATGTTTA ACCCTATGTA TGCCTTGTTC CGTACCTCAC CTGGTGATCG GGTCACATAC
    ACCATCAATC CATCTTCTCA CTGCAACCCC AACCACCTCA GCTACTTCAA GTTTGTTGGA
    CGCATCGTGG CCAAAGCTGT GTATGACAAC CGCCTCCTGG AGTGCTACTT TACTAGGTCC
    TTCTACAAAC ACATTTTGGG CAAGTCTGTC AGATATACAG ATATGGAGAG TGAAGACTAC
    CACTTTTACC AGGGCCTGGT TTATCTGCTG GAAAATGATG TCTCCACACT AGGCTATGAT
    CTCACTTTCA GCACTGAGGT CCAAGAGTTT GGAGTCTGTG AAGTTCGTGA CCTCAAACCC
    AATGGGGCCA ACATCTTGGT AACAGAGGAA AATAAGAAGG AATATGTACA CCTGGTGTGC
    CAGATGAGAA TGACAGGAGC CATCCGCAAA CAGCTGGCGG CTTTCTTAGA AGGTTTCTAT
    GAGATCATTC CAAAGCGCCT CATTTCCATC TTCACTGAGC AGGAGTTAGA GCTGCTCATA
    TCAGGACTGC CCACAATCGA CATTGACGAT TTGAAATCTA ACACTGAATA TCACAAGTAC
    CAGTCCAACT CTATTCAGAT CCAGTGGTTC TGGAGAGCAC TGCGTTCTTT CGATCAAGCT
    GACCGTGCCA AGTTCCTCCA GTTTGTCACA GGTACTTCCA AGGTGCCCCT GCAAGGTTTT
    GCTGCCCTTG AAGGCATGAA TGGCATTCAG AAGTTTCAAA TCCATCGAGA TGACAGGTCC
    ACAGACCGCC TGCCTTCAGC TCACACATGT TTTAATCAGC TGGACCTGCC TGCCTATGAG
    AGCTTTGAGA AGCTCCGCCA CATGCTACTA TTGGCCATCC AGGAGTGCTC TGAAGGCTTT
    GGGCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48124
    Clone ID Related Accession (Same CDS sequence) XM_024987899.1 , XM_010822002.2
    Accession Version XM_024987899.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
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    2641
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    9781
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    10261
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    10381
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    11521
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    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGCAGACATC
    CCCCCATCCC CAGGAAATAT CCCTACCACC CACCCACTGA TGGTACGCCA TGCAGATCAC
    AGTTCCCTGA CACTGGGCAG TGGCTCCTCT ACAACACGTC TCACCCAGGG CATTGGGCGC
    AGTCAAAGGA CCCTGAGACA GCTGACAGCC AACACAGGCC ATACCATTCA CGTTCACTAC
    CCTGGGAATC GCCAGCCCAA TCCTCCTCTT ATACTCCAGA GGTTGCTTGG TCCCTCTGCT
    GCTGCTGACA TCCTTCAGCT GAGCAGCAGC CTTCCCCTAC AAAGCCGAGG CCGAGCCCGC
    CTCCTAGTGG GCAACGATGA CGTCCACATC ATCGCCCGGT CTGATGATGA ACTACTGGAT
    GACTTTTTCC ATGATCAGAG CACAGCTACC AGCCAAGCAG GGACCCTATC CAGTATCCCT
    ACAGCCCTGA CTCGCTGGAC AGAGGAATGC AAAGTTCTTG ATGCTGAGAG CATGCATGAC
    TGTGTTTCAG TTGTTAAAGT GCCCATTGTC AATCATCTGG AATTCCTGAG GGATGAGGAG
    CTGGAAGAAA GGAGGGAGAA AAGGAGGAAA CAACTAGCTG AGGAAGAAAC AAAGATAACC
    GACAAAGGCA AAGAAGATAA GGAGAACAGG GATCAGAGTG CACAGTGTGC TGCATCTAAG
    ACAAATGACT CCACTGAACA GAACCTCTCA GATGGGACTC CTATGCCTGA CAGCTACCCA
    GCAACCCCAT CTTCAACTGA TGTAGCTACA TCTGAGCCCA GGGAGACCCT TGTCACTCTG
    CAGCCCTCCC AACAGCAACC AACACTCCCA ACCCCACCAG CCTTGGGAGA GATTCCTCAA
    GAGCTGCAGT CCCCAATTGG AGAAGGTGGG AGCTCTACAC AGCTATTGAT GCCTGTAGAG
    CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT GGAGAAGCAG AAACAACTCA GATGGAGCTA
    TCTCCAGCTC CCACTATAAC CTCTCTTTCC CCAGAGAGAG CTGAAGATTC TGATGCTCTG
    ACAGCTGTCA GCAGTCAACT GGAAGGCTCT CCCATGGATA CTAGCAGCCT GGCTTCCTGT
    ACCCTGGAGG AGGCTGTGGG TGACGCCCCA GCAACTGGCA GTTCTGAGCA GCCCACAGCA
    GGCAGCTCCA CTCCTGGGGA TGCCCCACCA GTTGTGACAG AAGTACAAGG CAGGGGTGAT
    GGGTCAGGGG AACCTACCCA GCCCCCTGAG GACAGCTCTC CCCCTGCATC CTCTGAGAGT
    TCTTCCACCA GAGATTCCGC GGTGGCCATT TCTGGAGCAG ATTCCCGGGG AATCCTGGAA
    GAGCCATTGC CTTCAACAAG CAGTGAAGAA GAAGATCCTC TTGCAGGTAT TAGTCTCCCC
    GAAGGTGTGG ACCCCTCTTT TCTGGCCGCT CTGCCTGATG ACATTCGCCG AGAAGTCCTA
    CAGAACCAGC TGGGCATTCG TCCACCAACA CGAACTGCCC CTTCAACAAA TAACTCAGCT
    CCTGCAGTGG TGGGGAATCC TGGTGTCACC GAAGTGAGCC CTGAGTTCCT GGCTGCTCTG
    CCTCCAGCCA TCCAGGAGGA AGTACTGGCA CAGCAGAGAG CTGAGCAACA GCGACGGGAA
    CTAGCACAAA ATGCCAGCTC AGACACCCCC ATGGACCCTG TGACCTTCAT CCAGACTCTG
    CCCTCAGACC TGCGCCGTAG TGTCCTAGAG GACATGGAGG ACAGTGTGTT GGCCGTGATG
    CCACCTGACA TCGCAGCTGA AGCTCAAGCC CTGAGGCGAG AGCAAGAAGC CCGGCAGAGG
    CAGCTCATGC ATGAGCGACT TTTTGGGCAC AGCAGCACCT CTGCACTCTC TGCCATTCTC
    CGAAGCCCAG CCTTCACCAG TCGCCTGAGT GGCAACCGTG GGGTCCAATA CACTCGCCTT
    GCTGTGCAAA GAGGTGGCAC CTTCCAGATG GGGGGTAGCA GCAGCCATAA TAGGCCTTCT
    GGCAGTAATG TGGACACCCT CCTACGTCTT CGAGGACGGC TCCTCCTGGA TCACGAAGCC
    CTGTCTTGTC TCCTGGTCCT ACTTTTTGTG GATGAGCCAA AACTCAATAC TAGCCGTCTT
    CACCGAGTAC TGAGAAATCT CTGCTACCAT GCCCAGACCC GCCACTGGGT TATCCGTAGC
    CTGCTTTCCA TCTTGCAGCG AAGCAGTGAG AGTGAGCTTT GCATTGAAAC ACCCAAGCTC
    TCCTCAAGTG AGGAAAAGGG CAAAAAGTCA AGCAAGAGCT GTGGGTCAAG CAGCCATGAG
    AACCGGCCCC TGGACCTGCT ACACAAGATG GAGTCTAAGA GTTCCAACCA GCTTTCCTGG
    CTCTCAGTAT CCATGGATGC AGCCCTAGGC TGCAGGACTA ATATATTCCA GATCCAGCGT
    TCAGGGGGTC GCAAACATAC TGAGAAACAT GCGAGTAGTG GCTCCACTGT CCATATCCAC
    CCCCAGGCTG CTCCTGTTGT CTGCAGACAT GTTCTGGATA CACTCATTCA ATTGGCCAAG
    GTGTTTCCCA GTCACTTCAC ACAGCAGCGG ACCAAAGAAA CAAACTGTGA GAGTGATCGG
    GAGAGGGGCA GTAAGGCCTG CAGCCCATGC TCTTCACAGT CCACAAGCAG TGGCATTTGC
    ACAGACTTCT GGGACTTATT GGTAAAACTG GACAACATGA ATGTCAGCCG GAAAGGCAAG
    AACTCCGTGA AGTCAGTGCC AGTGAGCGCT GGTGGTGAGG GGGAAACCTC CCCATACAGC
    CTCGAAGCTT CTCCACTGGG GCAACTCATG AACATGTTGT CACACCCAGT CATCCGCCGG
    AGCTCTCTCT TAACTGAGAA GCTCCTCAGA CTTCTTTCTC TCATCTCAAT TGCTCTCCCA
    GAAAACAAAG TGTCGGAAGC ACAGGCTAAT TCTGGCAGCA GTGCTTCCTC CACCACTGTT
    GCCACTTCAA CCACATCTAC CACCACTATC AGTGCCACCT CCAGCACATC TACTCTGCCT
    GCTGCAACCA CCCCTGTCAC TTCTGTTCCA GCCCTGGTTG CTGCCACGGC TATATCCACC
    ATTGCCGTAG CCGCTTCAAC CACAGTGACT ACCCCCACAA CTGCTACCAC TACTGTTTCA
    ACTGCTACTA CTACTAAGGC CAGCAAATCT CCAGCTAAAG TTGGTGATAT CGGCAGCAGC
    AGCAGCACAG ACTTTAAGAT GGTATCCTCT GGCCTCACTG AAAATCAGCT CCAGCTCTCT
    GTGGAAGTGT TGACATCCCA TTCTTGTTCT GAAGAAGGCC TAGAAGATGC AGCAAATGTG
    TTACTGCAGC TCTCTCGGGG GGACCCTGGG ACCCGGGACA CTGTTTTGAA GTTGCTACTG
    AATGGAGCCC GCCATCTGGG TTATACGCTT TGTAAACAGA TAGGAACTCT TCTGGCTGAG
    CTACGGGAAT ACAATCTGGA ACAGCAGCGG CGAGCCCAGT GTGAAACCCT CTCCCCTGAT
    GGCATGCCTG AGGAACAGCC ACAGACCACC AAGCTGAAAG GCAAAATGCA GAGCAGGTTT
    GACGTGGCTG AGAATGTGGT AATCGTGGCA TCTCAGAAGC GGCCTCTGGG TGGCCGGGAG
    CTCCAGCTGC CTTCTATGTC CATGTTGACA TCCAAGACAT CTACCCAGAA GTTCTTCTTG
    AGGGTACTGC AGGTCATCAT CCAGCTCCGG GATGACACGC GCCGTGCTAA CAAGAAGGCC
    AAGCAGACAG GCAGGCTAGG TTCCTCCGGT TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT
    GTTCGGCAGC TGGAGGCTGA GGCTGATGCC ATTATACAAA TGGTACGTGA GGGTCAGAGG
    GCGCGGAGAC AGCAACAAGC AGCAACGTCG GAGTCAAGCC AGTCAGAGGC ATCTGTCCGA
    AGGGAGGAGT CACCCATGGA TGTGGACCAG CCATCTCCCA GTGCTCAAGA TACTCAGTCC
    ATTGGCTCAG ATGGAACTCC ACAGGGAGAG AAGGAGAAGG AGGAGAGGCC ACCTGAGTTA
    CCCCTGCTCA GTGAGCAGCT GAACTTGGAT GAGCTGTGGG ACATGCTGGG AGAGTGTCTA
    AAGGAACTGG AGGAATCCCA TGACCAGCAT GCGGTGCTAG TGCTACAACC TGCTGTCGAG
    GCCTTCTTTC TGGTCCATGC AACAGAGCGG GAGAGCAAGC CTCCTGTCCG GGACACCCGT
    GAGAGCCAGC TGGCACACAT CAAGGACGAG CCACCTCCAC TCTCCCCTGC CCCTTTAACC
    CCAGCCACTC CTTCCTCCCT TGACCCGTTC TTCTCCCGGG AGCCCTCATC TATGCACATC
    TCCTCAAGCC TGCCCCCTGA CACACAGAAG TTTCTTCGTT TTGCAGAGAC TCATCGCACT
    GTGTTAAACC AGATCCTACG GCAGTCCACC ACCCACCTCG CTGATGGGCC TTTTGCTGTC
    CTGGTAGACT ACATTCGTGT CCTCGACTTC GATGTCAAGC GCAAATATTT CCGCCAAGAG
    CTGGAGCGTT TGGACGAGGG ACTCCGGAAA GAAGACATGG CTGTGCACGT TCGTCGTGAT
    CATGTCTTTG AAGACTCCTA TCGTGAGCTG CACCGGAAAT CCCCTGAAGA AATGAAGAAT
    CGATTGTATA TAGTATTTGA AGGAGAAGAA GGGCAGGATG CTGGAGGGCT CCTGCGGGAG
    TGGTATATGA TCATCTCTCG AGAGATGTTT AACCCTATGT ATGCCTTGTT CCGTACCTCA
    CCTGGTGATC GGGTCACATA CACCATCAAT CCATCTTCTC ACTGCAACCC CAACCACCTC
    AGCTACTTCA AGTTTGTTGG ACGCATCGTG GCCAAAGCTG TGTATGACAA CCGCCTCCTG
    GAGTGCTACT TTACTAGGTC CTTCTACAAA CACATTTTGG GCAAGTCTGT CAGATATACA
    GATATGGAGA GTGAAGACTA CCACTTTTAC CAGGGCCTGG TTTATCTGCT GGAAAATGAT
    GTCTCCACAC TAGGCTATGA TCTCACTTTC AGCACTGAGG TCCAAGAGTT TGGAGTCTGT
    GAAGTTCGTG ACCTCAAACC CAATGGGGCC AACATCTTGG TAACAGAGGA AAATAAGAAG
    GAATATGTAC ACCTGGTGTG CCAGATGAGA ATGACAGGAG CCATCCGCAA ACAGCTGGCG
    GCTTTCTTAG AAGGTTTCTA TGAGATCATT CCAAAGCGCC TCATTTCCAT CTTCACTGAG
    CAGGAGTTAG AGCTGCTCAT ATCAGGACTG CCCACAATCG ACATTGACGA TTTGAAATCT
    AACACTGAAT ATCACAAGTA CCAGTCCAAC TCTATTCAGA TCCAGTGGTT CTGGAGAGCA
    CTGCGTTCTT TCGATCAAGC TGACCGTGCC AAGTTCCTCC AGTTTGTCAC AGGTACTTCC
    AAGGTGCCCC TGCAAGGTTT TGCTGCCCTT GAAGGCATGA ATGGCATTCA GAAGTTTCAA
    ATCCATCGAG ATGACAGGTC CACAGACCGC CTGCCTTCAG CTCACACATG TTTTAATCAG
    CTGGACCTGC CTGCCTATGA GAGCTTTGAG AAGCTCCGCC ACATGCTACT ATTGGCCATC
    CAGGAGTGCT CTGAAGGCTT TGGGCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843667.1
    CDS443..13315
    Translation

    Target ORF information:

    RefSeq Version XM_024987899.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987899.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGCAGACATC
    CCCCCATCCC CAGGAAATAT CCCTACCACC CACCCACTGA TGGTACGCCA TGCAGATCAC
    AGTTCCCTGA CACTGGGCAG TGGCTCCTCT ACAACACGTC TCACCCAGGG CATTGGGCGC
    AGTCAAAGGA CCCTGAGACA GCTGACAGCC AACACAGGCC ATACCATTCA CGTTCACTAC
    CCTGGGAATC GCCAGCCCAA TCCTCCTCTT ATACTCCAGA GGTTGCTTGG TCCCTCTGCT
    GCTGCTGACA TCCTTCAGCT GAGCAGCAGC CTTCCCCTAC AAAGCCGAGG CCGAGCCCGC
    CTCCTAGTGG GCAACGATGA CGTCCACATC ATCGCCCGGT CTGATGATGA ACTACTGGAT
    GACTTTTTCC ATGATCAGAG CACAGCTACC AGCCAAGCAG GGACCCTATC CAGTATCCCT
    ACAGCCCTGA CTCGCTGGAC AGAGGAATGC AAAGTTCTTG ATGCTGAGAG CATGCATGAC
    TGTGTTTCAG TTGTTAAAGT GCCCATTGTC AATCATCTGG AATTCCTGAG GGATGAGGAG
    CTGGAAGAAA GGAGGGAGAA AAGGAGGAAA CAACTAGCTG AGGAAGAAAC AAAGATAACC
    GACAAAGGCA AAGAAGATAA GGAGAACAGG GATCAGAGTG CACAGTGTGC TGCATCTAAG
    ACAAATGACT CCACTGAACA GAACCTCTCA GATGGGACTC CTATGCCTGA CAGCTACCCA
    GCAACCCCAT CTTCAACTGA TGTAGCTACA TCTGAGCCCA GGGAGACCCT TGTCACTCTG
    CAGCCCTCCC AACAGCAACC AACACTCCCA ACCCCACCAG CCTTGGGAGA GATTCCTCAA
    GAGCTGCAGT CCCCAATTGG AGAAGGTGGG AGCTCTACAC AGCTATTGAT GCCTGTAGAG
    CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT GGAGAAGCAG AAACAACTCA GATGGAGCTA
    TCTCCAGCTC CCACTATAAC CTCTCTTTCC CCAGAGAGAG CTGAAGATTC TGATGCTCTG
    ACAGCTGTCA GCAGTCAACT GGAAGGCTCT CCCATGGATA CTAGCAGCCT GGCTTCCTGT
    ACCCTGGAGG AGGCTGTGGG TGACGCCCCA GCAACTGGCA GTTCTGAGCA GCCCACAGCA
    GGCAGCTCCA CTCCTGGGGA TGCCCCACCA GTTGTGACAG AAGTACAAGG CAGGGGTGAT
    GGGTCAGGGG AACCTACCCA GCCCCCTGAG GACAGCTCTC CCCCTGCATC CTCTGAGAGT
    TCTTCCACCA GAGATTCCGC GGTGGCCATT TCTGGAGCAG ATTCCCGGGG AATCCTGGAA
    GAGCCATTGC CTTCAACAAG CAGTGAAGAA GAAGATCCTC TTGCAGGTAT TAGTCTCCCC
    GAAGGTGTGG ACCCCTCTTT TCTGGCCGCT CTGCCTGATG ACATTCGCCG AGAAGTCCTA
    CAGAACCAGC TGGGCATTCG TCCACCAACA CGAACTGCCC CTTCAACAAA TAACTCAGCT
    CCTGCAGTGG TGGGGAATCC TGGTGTCACC GAAGTGAGCC CTGAGTTCCT GGCTGCTCTG
    CCTCCAGCCA TCCAGGAGGA AGTACTGGCA CAGCAGAGAG CTGAGCAACA GCGACGGGAA
    CTAGCACAAA ATGCCAGCTC AGACACCCCC ATGGACCCTG TGACCTTCAT CCAGACTCTG
    CCCTCAGACC TGCGCCGTAG TGTCCTAGAG GACATGGAGG ACAGTGTGTT GGCCGTGATG
    CCACCTGACA TCGCAGCTGA AGCTCAAGCC CTGAGGCGAG AGCAAGAAGC CCGGCAGAGG
    CAGCTCATGC ATGAGCGACT TTTTGGGCAC AGCAGCACCT CTGCACTCTC TGCCATTCTC
    CGAAGCCCAG CCTTCACCAG TCGCCTGAGT GGCAACCGTG GGGTCCAATA CACTCGCCTT
    GCTGTGCAAA GAGGTGGCAC CTTCCAGATG GGGGGTAGCA GCAGCCATAA TAGGCCTTCT
    GGCAGTAATG TGGACACCCT CCTACGTCTT CGAGGACGGC TCCTCCTGGA TCACGAAGCC
    CTGTCTTGTC TCCTGGTCCT ACTTTTTGTG GATGAGCCAA AACTCAATAC TAGCCGTCTT
    CACCGAGTAC TGAGAAATCT CTGCTACCAT GCCCAGACCC GCCACTGGGT TATCCGTAGC
    CTGCTTTCCA TCTTGCAGCG AAGCAGTGAG AGTGAGCTTT GCATTGAAAC ACCCAAGCTC
    TCCTCAAGTG AGGAAAAGGG CAAAAAGTCA AGCAAGAGCT GTGGGTCAAG CAGCCATGAG
    AACCGGCCCC TGGACCTGCT ACACAAGATG GAGTCTAAGA GTTCCAACCA GCTTTCCTGG
    CTCTCAGTAT CCATGGATGC AGCCCTAGGC TGCAGGACTA ATATATTCCA GATCCAGCGT
    TCAGGGGGTC GCAAACATAC TGAGAAACAT GCGAGTAGTG GCTCCACTGT CCATATCCAC
    CCCCAGGCTG CTCCTGTTGT CTGCAGACAT GTTCTGGATA CACTCATTCA ATTGGCCAAG
    GTGTTTCCCA GTCACTTCAC ACAGCAGCGG ACCAAAGAAA CAAACTGTGA GAGTGATCGG
    GAGAGGGGCA GTAAGGCCTG CAGCCCATGC TCTTCACAGT CCACAAGCAG TGGCATTTGC
    ACAGACTTCT GGGACTTATT GGTAAAACTG GACAACATGA ATGTCAGCCG GAAAGGCAAG
    AACTCCGTGA AGTCAGTGCC AGTGAGCGCT GGTGGTGAGG GGGAAACCTC CCCATACAGC
    CTCGAAGCTT CTCCACTGGG GCAACTCATG AACATGTTGT CACACCCAGT CATCCGCCGG
    AGCTCTCTCT TAACTGAGAA GCTCCTCAGA CTTCTTTCTC TCATCTCAAT TGCTCTCCCA
    GAAAACAAAG TGTCGGAAGC ACAGGCTAAT TCTGGCAGCA GTGCTTCCTC CACCACTGTT
    GCCACTTCAA CCACATCTAC CACCACTATC AGTGCCACCT CCAGCACATC TACTCTGCCT
    GCTGCAACCA CCCCTGTCAC TTCTGTTCCA GCCCTGGTTG CTGCCACGGC TATATCCACC
    ATTGCCGTAG CCGCTTCAAC CACAGTGACT ACCCCCACAA CTGCTACCAC TACTGTTTCA
    ACTGCTACTA CTACTAAGGC CAGCAAATCT CCAGCTAAAG TTGGTGATAT CGGCAGCAGC
    AGCAGCACAG ACTTTAAGAT GGTATCCTCT GGCCTCACTG AAAATCAGCT CCAGCTCTCT
    GTGGAAGTGT TGACATCCCA TTCTTGTTCT GAAGAAGGCC TAGAAGATGC AGCAAATGTG
    TTACTGCAGC TCTCTCGGGG GGACCCTGGG ACCCGGGACA CTGTTTTGAA GTTGCTACTG
    AATGGAGCCC GCCATCTGGG TTATACGCTT TGTAAACAGA TAGGAACTCT TCTGGCTGAG
    CTACGGGAAT ACAATCTGGA ACAGCAGCGG CGAGCCCAGT GTGAAACCCT CTCCCCTGAT
    GGCATGCCTG AGGAACAGCC ACAGACCACC AAGCTGAAAG GCAAAATGCA GAGCAGGTTT
    GACGTGGCTG AGAATGTGGT AATCGTGGCA TCTCAGAAGC GGCCTCTGGG TGGCCGGGAG
    CTCCAGCTGC CTTCTATGTC CATGTTGACA TCCAAGACAT CTACCCAGAA GTTCTTCTTG
    AGGGTACTGC AGGTCATCAT CCAGCTCCGG GATGACACGC GCCGTGCTAA CAAGAAGGCC
    AAGCAGACAG GCAGGCTAGG TTCCTCCGGT TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT
    GTTCGGCAGC TGGAGGCTGA GGCTGATGCC ATTATACAAA TGGTACGTGA GGGTCAGAGG
    GCGCGGAGAC AGCAACAAGC AGCAACGTCG GAGTCAAGCC AGTCAGAGGC ATCTGTCCGA
    AGGGAGGAGT CACCCATGGA TGTGGACCAG CCATCTCCCA GTGCTCAAGA TACTCAGTCC
    ATTGGCTCAG ATGGAACTCC ACAGGGAGAG AAGGAGAAGG AGGAGAGGCC ACCTGAGTTA
    CCCCTGCTCA GTGAGCAGCT GAACTTGGAT GAGCTGTGGG ACATGCTGGG AGAGTGTCTA
    AAGGAACTGG AGGAATCCCA TGACCAGCAT GCGGTGCTAG TGCTACAACC TGCTGTCGAG
    GCCTTCTTTC TGGTCCATGC AACAGAGCGG GAGAGCAAGC CTCCTGTCCG GGACACCCGT
    GAGAGCCAGC TGGCACACAT CAAGGACGAG CCACCTCCAC TCTCCCCTGC CCCTTTAACC
    CCAGCCACTC CTTCCTCCCT TGACCCGTTC TTCTCCCGGG AGCCCTCATC TATGCACATC
    TCCTCAAGCC TGCCCCCTGA CACACAGAAG TTTCTTCGTT TTGCAGAGAC TCATCGCACT
    GTGTTAAACC AGATCCTACG GCAGTCCACC ACCCACCTCG CTGATGGGCC TTTTGCTGTC
    CTGGTAGACT ACATTCGTGT CCTCGACTTC GATGTCAAGC GCAAATATTT CCGCCAAGAG
    CTGGAGCGTT TGGACGAGGG ACTCCGGAAA GAAGACATGG CTGTGCACGT TCGTCGTGAT
    CATGTCTTTG AAGACTCCTA TCGTGAGCTG CACCGGAAAT CCCCTGAAGA AATGAAGAAT
    CGATTGTATA TAGTATTTGA AGGAGAAGAA GGGCAGGATG CTGGAGGGCT CCTGCGGGAG
    TGGTATATGA TCATCTCTCG AGAGATGTTT AACCCTATGT ATGCCTTGTT CCGTACCTCA
    CCTGGTGATC GGGTCACATA CACCATCAAT CCATCTTCTC ACTGCAACCC CAACCACCTC
    AGCTACTTCA AGTTTGTTGG ACGCATCGTG GCCAAAGCTG TGTATGACAA CCGCCTCCTG
    GAGTGCTACT TTACTAGGTC CTTCTACAAA CACATTTTGG GCAAGTCTGT CAGATATACA
    GATATGGAGA GTGAAGACTA CCACTTTTAC CAGGGCCTGG TTTATCTGCT GGAAAATGAT
    GTCTCCACAC TAGGCTATGA TCTCACTTTC AGCACTGAGG TCCAAGAGTT TGGAGTCTGT
    GAAGTTCGTG ACCTCAAACC CAATGGGGCC AACATCTTGG TAACAGAGGA AAATAAGAAG
    GAATATGTAC ACCTGGTGTG CCAGATGAGA ATGACAGGAG CCATCCGCAA ACAGCTGGCG
    GCTTTCTTAG AAGGTTTCTA TGAGATCATT CCAAAGCGCC TCATTTCCAT CTTCACTGAG
    CAGGAGTTAG AGCTGCTCAT ATCAGGACTG CCCACAATCG ACATTGACGA TTTGAAATCT
    AACACTGAAT ATCACAAGTA CCAGTCCAAC TCTATTCAGA TCCAGTGGTT CTGGAGAGCA
    CTGCGTTCTT TCGATCAAGC TGACCGTGCC AAGTTCCTCC AGTTTGTCAC AGGTACTTCC
    AAGGTGCCCC TGCAAGGTTT TGCTGCCCTT GAAGGCATGA ATGGCATTCA GAAGTTTCAA
    ATCCATCGAG ATGACAGGTC CACAGACCGC CTGCCTTCAG CTCACACATG TTTTAATCAG
    CTGGACCTGC CTGCCTATGA GAGCTTTGAG AAGCTCCGCC ACATGCTACT ATTGGCCATC
    CAGGAGTGCT CTGAAGGCTT TGGGCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48124
    Clone ID Related Accession (Same CDS sequence) XM_024987899.1 , XM_010822002.2
    Accession Version XM_010822002.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822002.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGCAGACATC
    CCCCCATCCC CAGGAAATAT CCCTACCACC CACCCACTGA TGGTACGCCA TGCAGATCAC
    AGTTCCCTGA CACTGGGCAG TGGCTCCTCT ACAACACGTC TCACCCAGGG CATTGGGCGC
    AGTCAAAGGA CCCTGAGACA GCTGACAGCC AACACAGGCC ATACCATTCA CGTTCACTAC
    CCTGGGAATC GCCAGCCCAA TCCTCCTCTT ATACTCCAGA GGTTGCTTGG TCCCTCTGCT
    GCTGCTGACA TCCTTCAGCT GAGCAGCAGC CTTCCCCTAC AAAGCCGAGG CCGAGCCCGC
    CTCCTAGTGG GCAACGATGA CGTCCACATC ATCGCCCGGT CTGATGATGA ACTACTGGAT
    GACTTTTTCC ATGATCAGAG CACAGCTACC AGCCAAGCAG GGACCCTATC CAGTATCCCT
    ACAGCCCTGA CTCGCTGGAC AGAGGAATGC AAAGTTCTTG ATGCTGAGAG CATGCATGAC
    TGTGTTTCAG TTGTTAAAGT GCCCATTGTC AATCATCTGG AATTCCTGAG GGATGAGGAG
    CTGGAAGAAA GGAGGGAGAA AAGGAGGAAA CAACTAGCTG AGGAAGAAAC AAAGATAACC
    GACAAAGGCA AAGAAGATAA GGAGAACAGG GATCAGAGTG CACAGTGTGC TGCATCTAAG
    ACAAATGACT CCACTGAACA GAACCTCTCA GATGGGACTC CTATGCCTGA CAGCTACCCA
    GCAACCCCAT CTTCAACTGA TGTAGCTACA TCTGAGCCCA GGGAGACCCT TGTCACTCTG
    CAGCCCTCCC AACAGCAACC AACACTCCCA ACCCCACCAG CCTTGGGAGA GATTCCTCAA
    GAGCTGCAGT CCCCAATTGG AGAAGGTGGG AGCTCTACAC AGCTATTGAT GCCTGTAGAG
    CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT GGAGAAGCAG AAACAACTCA GATGGAGCTA
    TCTCCAGCTC CCACTATAAC CTCTCTTTCC CCAGAGAGAG CTGAAGATTC TGATGCTCTG
    ACAGCTGTCA GCAGTCAACT GGAAGGCTCT CCCATGGATA CTAGCAGCCT GGCTTCCTGT
    ACCCTGGAGG AGGCTGTGGG TGACGCCCCA GCAACTGGCA GTTCTGAGCA GCCCACAGCA
    GGCAGCTCCA CTCCTGGGGA TGCCCCACCA GTTGTGACAG AAGTACAAGG CAGGGGTGAT
    GGGTCAGGGG AACCTACCCA GCCCCCTGAG GACAGCTCTC CCCCTGCATC CTCTGAGAGT
    TCTTCCACCA GAGATTCCGC GGTGGCCATT TCTGGAGCAG ATTCCCGGGG AATCCTGGAA
    GAGCCATTGC CTTCAACAAG CAGTGAAGAA GAAGATCCTC TTGCAGGTAT TAGTCTCCCC
    GAAGGTGTGG ACCCCTCTTT TCTGGCCGCT CTGCCTGATG ACATTCGCCG AGAAGTCCTA
    CAGAACCAGC TGGGCATTCG TCCACCAACA CGAACTGCCC CTTCAACAAA TAACTCAGCT
    CCTGCAGTGG TGGGGAATCC TGGTGTCACC GAAGTGAGCC CTGAGTTCCT GGCTGCTCTG
    CCTCCAGCCA TCCAGGAGGA AGTACTGGCA CAGCAGAGAG CTGAGCAACA GCGACGGGAA
    CTAGCACAAA ATGCCAGCTC AGACACCCCC ATGGACCCTG TGACCTTCAT CCAGACTCTG
    CCCTCAGACC TGCGCCGTAG TGTCCTAGAG GACATGGAGG ACAGTGTGTT GGCCGTGATG
    CCACCTGACA TCGCAGCTGA AGCTCAAGCC CTGAGGCGAG AGCAAGAAGC CCGGCAGAGG
    CAGCTCATGC ATGAGCGACT TTTTGGGCAC AGCAGCACCT CTGCACTCTC TGCCATTCTC
    CGAAGCCCAG CCTTCACCAG TCGCCTGAGT GGCAACCGTG GGGTCCAATA CACTCGCCTT
    GCTGTGCAAA GAGGTGGCAC CTTCCAGATG GGGGGTAGCA GCAGCCATAA TAGGCCTTCT
    GGCAGTAATG TGGACACCCT CCTACGTCTT CGAGGACGGC TCCTCCTGGA TCACGAAGCC
    CTGTCTTGTC TCCTGGTCCT ACTTTTTGTG GATGAGCCAA AACTCAATAC TAGCCGTCTT
    CACCGAGTAC TGAGAAATCT CTGCTACCAT GCCCAGACCC GCCACTGGGT TATCCGTAGC
    CTGCTTTCCA TCTTGCAGCG AAGCAGTGAG AGTGAGCTTT GCATTGAAAC ACCCAAGCTC
    TCCTCAAGTG AGGAAAAGGG CAAAAAGTCA AGCAAGAGCT GTGGGTCAAG CAGCCATGAG
    AACCGGCCCC TGGACCTGCT ACACAAGATG GAGTCTAAGA GTTCCAACCA GCTTTCCTGG
    CTCTCAGTAT CCATGGATGC AGCCCTAGGC TGCAGGACTA ATATATTCCA GATCCAGCGT
    TCAGGGGGTC GCAAACATAC TGAGAAACAT GCGAGTAGTG GCTCCACTGT CCATATCCAC
    CCCCAGGCTG CTCCTGTTGT CTGCAGACAT GTTCTGGATA CACTCATTCA ATTGGCCAAG
    GTGTTTCCCA GTCACTTCAC ACAGCAGCGG ACCAAAGAAA CAAACTGTGA GAGTGATCGG
    GAGAGGGGCA GTAAGGCCTG CAGCCCATGC TCTTCACAGT CCACAAGCAG TGGCATTTGC
    ACAGACTTCT GGGACTTATT GGTAAAACTG GACAACATGA ATGTCAGCCG GAAAGGCAAG
    AACTCCGTGA AGTCAGTGCC AGTGAGCGCT GGTGGTGAGG GGGAAACCTC CCCATACAGC
    CTCGAAGCTT CTCCACTGGG GCAACTCATG AACATGTTGT CACACCCAGT CATCCGCCGG
    AGCTCTCTCT TAACTGAGAA GCTCCTCAGA CTTCTTTCTC TCATCTCAAT TGCTCTCCCA
    GAAAACAAAG TGTCGGAAGC ACAGGCTAAT TCTGGCAGCA GTGCTTCCTC CACCACTGTT
    GCCACTTCAA CCACATCTAC CACCACTATC AGTGCCACCT CCAGCACATC TACTCTGCCT
    GCTGCAACCA CCCCTGTCAC TTCTGTTCCA GCCCTGGTTG CTGCCACGGC TATATCCACC
    ATTGCCGTAG CCGCTTCAAC CACAGTGACT ACCCCCACAA CTGCTACCAC TACTGTTTCA
    ACTGCTACTA CTACTAAGGC CAGCAAATCT CCAGCTAAAG TTGGTGATAT CGGCAGCAGC
    AGCAGCACAG ACTTTAAGAT GGTATCCTCT GGCCTCACTG AAAATCAGCT CCAGCTCTCT
    GTGGAAGTGT TGACATCCCA TTCTTGTTCT GAAGAAGGCC TAGAAGATGC AGCAAATGTG
    TTACTGCAGC TCTCTCGGGG GGACCCTGGG ACCCGGGACA CTGTTTTGAA GTTGCTACTG
    AATGGAGCCC GCCATCTGGG TTATACGCTT TGTAAACAGA TAGGAACTCT TCTGGCTGAG
    CTACGGGAAT ACAATCTGGA ACAGCAGCGG CGAGCCCAGT GTGAAACCCT CTCCCCTGAT
    GGCATGCCTG AGGAACAGCC ACAGACCACC AAGCTGAAAG GCAAAATGCA GAGCAGGTTT
    GACGTGGCTG AGAATGTGGT AATCGTGGCA TCTCAGAAGC GGCCTCTGGG TGGCCGGGAG
    CTCCAGCTGC CTTCTATGTC CATGTTGACA TCCAAGACAT CTACCCAGAA GTTCTTCTTG
    AGGGTACTGC AGGTCATCAT CCAGCTCCGG GATGACACGC GCCGTGCTAA CAAGAAGGCC
    AAGCAGACAG GCAGGCTAGG TTCCTCCGGT TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT
    GTTCGGCAGC TGGAGGCTGA GGCTGATGCC ATTATACAAA TGGTACGTGA GGGTCAGAGG
    GCGCGGAGAC AGCAACAAGC AGCAACGTCG GAGTCAAGCC AGTCAGAGGC ATCTGTCCGA
    AGGGAGGAGT CACCCATGGA TGTGGACCAG CCATCTCCCA GTGCTCAAGA TACTCAGTCC
    ATTGGCTCAG ATGGAACTCC ACAGGGAGAG AAGGAGAAGG AGGAGAGGCC ACCTGAGTTA
    CCCCTGCTCA GTGAGCAGCT GAACTTGGAT GAGCTGTGGG ACATGCTGGG AGAGTGTCTA
    AAGGAACTGG AGGAATCCCA TGACCAGCAT GCGGTGCTAG TGCTACAACC TGCTGTCGAG
    GCCTTCTTTC TGGTCCATGC AACAGAGCGG GAGAGCAAGC CTCCTGTCCG GGACACCCGT
    GAGAGCCAGC TGGCACACAT CAAGGACGAG CCACCTCCAC TCTCCCCTGC CCCTTTAACC
    CCAGCCACTC CTTCCTCCCT TGACCCGTTC TTCTCCCGGG AGCCCTCATC TATGCACATC
    TCCTCAAGCC TGCCCCCTGA CACACAGAAG TTTCTTCGTT TTGCAGAGAC TCATCGCACT
    GTGTTAAACC AGATCCTACG GCAGTCCACC ACCCACCTCG CTGATGGGCC TTTTGCTGTC
    CTGGTAGACT ACATTCGTGT CCTCGACTTC GATGTCAAGC GCAAATATTT CCGCCAAGAG
    CTGGAGCGTT TGGACGAGGG ACTCCGGAAA GAAGACATGG CTGTGCACGT TCGTCGTGAT
    CATGTCTTTG AAGACTCCTA TCGTGAGCTG CACCGGAAAT CCCCTGAAGA AATGAAGAAT
    CGATTGTATA TAGTATTTGA AGGAGAAGAA GGGCAGGATG CTGGAGGGCT CCTGCGGGAG
    TGGTATATGA TCATCTCTCG AGAGATGTTT AACCCTATGT ATGCCTTGTT CCGTACCTCA
    CCTGGTGATC GGGTCACATA CACCATCAAT CCATCTTCTC ACTGCAACCC CAACCACCTC
    AGCTACTTCA AGTTTGTTGG ACGCATCGTG GCCAAAGCTG TGTATGACAA CCGCCTCCTG
    GAGTGCTACT TTACTAGGTC CTTCTACAAA CACATTTTGG GCAAGTCTGT CAGATATACA
    GATATGGAGA GTGAAGACTA CCACTTTTAC CAGGGCCTGG TTTATCTGCT GGAAAATGAT
    GTCTCCACAC TAGGCTATGA TCTCACTTTC AGCACTGAGG TCCAAGAGTT TGGAGTCTGT
    GAAGTTCGTG ACCTCAAACC CAATGGGGCC AACATCTTGG TAACAGAGGA AAATAAGAAG
    GAATATGTAC ACCTGGTGTG CCAGATGAGA ATGACAGGAG CCATCCGCAA ACAGCTGGCG
    GCTTTCTTAG AAGGTTTCTA TGAGATCATT CCAAAGCGCC TCATTTCCAT CTTCACTGAG
    CAGGAGTTAG AGCTGCTCAT ATCAGGACTG CCCACAATCG ACATTGACGA TTTGAAATCT
    AACACTGAAT ATCACAAGTA CCAGTCCAAC TCTATTCAGA TCCAGTGGTT CTGGAGAGCA
    CTGCGTTCTT TCGATCAAGC TGACCGTGCC AAGTTCCTCC AGTTTGTCAC AGGTACTTCC
    AAGGTGCCCC TGCAAGGTTT TGCTGCCCTT GAAGGCATGA ATGGCATTCA GAAGTTTCAA
    ATCCATCGAG ATGACAGGTC CACAGACCGC CTGCCTTCAG CTCACACATG TTTTAATCAG
    CTGGACCTGC CTGCCTATGA GAGCTTTGAG AAGCTCCGCC ACATGCTACT ATTGGCCATC
    CAGGAGTGCT CTGAAGGCTT TGGGCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820304.1
    CDS735..13607
    Translation

    Target ORF information:

    RefSeq Version XM_010822002.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010822002.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACTCCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTTTTGGA ACTGCAGCAG
    ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGATCT GTTGGACCGC
    TTCGATGGGA TTCTGGCAGA TGCTGGACAG ACAGTGGAGA ACATGTCATG GATGCTCGTG
    TGTGATAGGC CGGAAAAAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTACTCA TTGAGTACAG CTTTTCCCGG CATCTGTATA GTTCCATAGA GCACTTGACA
    ACTTTATTGG CTTCTTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAACCT TCTGTATGTG
    TTTAGCAAAA GATCAAATTA TATCACACGT CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AGCATTTGGC AGAGAGCTGG GGTGGTAAGG AGAATGGCTT TGGACTAGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCCACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCTGAGGTC AAAATCGAGA AAAGGACATC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAGTCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAAGCTGT TCAGGCCAGG
    CTACATGCAA TATCTATATT AGTGTTTATT TCTCCTTTTG CAGTGTATTC CAATGCCTTG
    CAGGAGTCAG CAAACAGTAT CTTGTATAAT GGGTTGATAG AGGAGTTGGT AGATGTCCTT
    CAGATAACAG ATAAGCAGCT TATGGAGATA AAAGCTGCTT CTTTACGAAC GTTAACATCA
    ATTGTCCACT TAGAGAGAAC TCCCAAACTC AGCAGTATTA TTGATTGTAC TGGAACTGCC
    TCCTACCATG GATTTTTGCC TGTACTTGTG CGAAACTGTA TCCAGGCCAT GATTGACCCT
    TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG
    GCAAGCTACG ATGCTGGTGG CGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG
    AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCTGTC
    AGAGTGGTTG ACCTTATCAC CAACCTGGAC ATGGCAGCTT TTCAATCCCA TAGTGGACTT
    TCTATCTTCA TTTATAGACT TGAGCATGAA GTTGATTTGT GCCGAAAAGA ATGCCCTTTT
    GTGATCAAGC CAAAGATCCA GAGACCCAGT ACTGCACAAG AAGGAGAAGA AATGGAAACT
    GATATGGATG TTACAGATGT GACTATGGAA AGTAGTCCCA GCTCATCCAT CTCTATGGAG
    CACCGGCTGG ATGTTGAATT AAGGGCATCA AGTTCCAGCA GCAGTAGCAT CTCCTCTGGC
    CCTGGCCCTC GTCCTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGCAGACATC
    CCCCCATCCC CAGGAAATAT CCCTACCACC CACCCACTGA TGGTACGCCA TGCAGATCAC
    AGTTCCCTGA CACTGGGCAG TGGCTCCTCT ACAACACGTC TCACCCAGGG CATTGGGCGC
    AGTCAAAGGA CCCTGAGACA GCTGACAGCC AACACAGGCC ATACCATTCA CGTTCACTAC
    CCTGGGAATC GCCAGCCCAA TCCTCCTCTT ATACTCCAGA GGTTGCTTGG TCCCTCTGCT
    GCTGCTGACA TCCTTCAGCT GAGCAGCAGC CTTCCCCTAC AAAGCCGAGG CCGAGCCCGC
    CTCCTAGTGG GCAACGATGA CGTCCACATC ATCGCCCGGT CTGATGATGA ACTACTGGAT
    GACTTTTTCC ATGATCAGAG CACAGCTACC AGCCAAGCAG GGACCCTATC CAGTATCCCT
    ACAGCCCTGA CTCGCTGGAC AGAGGAATGC AAAGTTCTTG ATGCTGAGAG CATGCATGAC
    TGTGTTTCAG TTGTTAAAGT GCCCATTGTC AATCATCTGG AATTCCTGAG GGATGAGGAG
    CTGGAAGAAA GGAGGGAGAA AAGGAGGAAA CAACTAGCTG AGGAAGAAAC AAAGATAACC
    GACAAAGGCA AAGAAGATAA GGAGAACAGG GATCAGAGTG CACAGTGTGC TGCATCTAAG
    ACAAATGACT CCACTGAACA GAACCTCTCA GATGGGACTC CTATGCCTGA CAGCTACCCA
    GCAACCCCAT CTTCAACTGA TGTAGCTACA TCTGAGCCCA GGGAGACCCT TGTCACTCTG
    CAGCCCTCCC AACAGCAACC AACACTCCCA ACCCCACCAG CCTTGGGAGA GATTCCTCAA
    GAGCTGCAGT CCCCAATTGG AGAAGGTGGG AGCTCTACAC AGCTATTGAT GCCTGTAGAG
    CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT GGAGAAGCAG AAACAACTCA GATGGAGCTA
    TCTCCAGCTC CCACTATAAC CTCTCTTTCC CCAGAGAGAG CTGAAGATTC TGATGCTCTG
    ACAGCTGTCA GCAGTCAACT GGAAGGCTCT CCCATGGATA CTAGCAGCCT GGCTTCCTGT
    ACCCTGGAGG AGGCTGTGGG TGACGCCCCA GCAACTGGCA GTTCTGAGCA GCCCACAGCA
    GGCAGCTCCA CTCCTGGGGA TGCCCCACCA GTTGTGACAG AAGTACAAGG CAGGGGTGAT
    GGGTCAGGGG AACCTACCCA GCCCCCTGAG GACAGCTCTC CCCCTGCATC CTCTGAGAGT
    TCTTCCACCA GAGATTCCGC GGTGGCCATT TCTGGAGCAG ATTCCCGGGG AATCCTGGAA
    GAGCCATTGC CTTCAACAAG CAGTGAAGAA GAAGATCCTC TTGCAGGTAT TAGTCTCCCC
    GAAGGTGTGG ACCCCTCTTT TCTGGCCGCT CTGCCTGATG ACATTCGCCG AGAAGTCCTA
    CAGAACCAGC TGGGCATTCG TCCACCAACA CGAACTGCCC CTTCAACAAA TAACTCAGCT
    CCTGCAGTGG TGGGGAATCC TGGTGTCACC GAAGTGAGCC CTGAGTTCCT GGCTGCTCTG
    CCTCCAGCCA TCCAGGAGGA AGTACTGGCA CAGCAGAGAG CTGAGCAACA GCGACGGGAA
    CTAGCACAAA ATGCCAGCTC AGACACCCCC ATGGACCCTG TGACCTTCAT CCAGACTCTG
    CCCTCAGACC TGCGCCGTAG TGTCCTAGAG GACATGGAGG ACAGTGTGTT GGCCGTGATG
    CCACCTGACA TCGCAGCTGA AGCTCAAGCC CTGAGGCGAG AGCAAGAAGC CCGGCAGAGG
    CAGCTCATGC ATGAGCGACT TTTTGGGCAC AGCAGCACCT CTGCACTCTC TGCCATTCTC
    CGAAGCCCAG CCTTCACCAG TCGCCTGAGT GGCAACCGTG GGGTCCAATA CACTCGCCTT
    GCTGTGCAAA GAGGTGGCAC CTTCCAGATG GGGGGTAGCA GCAGCCATAA TAGGCCTTCT
    GGCAGTAATG TGGACACCCT CCTACGTCTT CGAGGACGGC TCCTCCTGGA TCACGAAGCC
    CTGTCTTGTC TCCTGGTCCT ACTTTTTGTG GATGAGCCAA AACTCAATAC TAGCCGTCTT
    CACCGAGTAC TGAGAAATCT CTGCTACCAT GCCCAGACCC GCCACTGGGT TATCCGTAGC
    CTGCTTTCCA TCTTGCAGCG AAGCAGTGAG AGTGAGCTTT GCATTGAAAC ACCCAAGCTC
    TCCTCAAGTG AGGAAAAGGG CAAAAAGTCA AGCAAGAGCT GTGGGTCAAG CAGCCATGAG
    AACCGGCCCC TGGACCTGCT ACACAAGATG GAGTCTAAGA GTTCCAACCA GCTTTCCTGG
    CTCTCAGTAT CCATGGATGC AGCCCTAGGC TGCAGGACTA ATATATTCCA GATCCAGCGT
    TCAGGGGGTC GCAAACATAC TGAGAAACAT GCGAGTAGTG GCTCCACTGT CCATATCCAC
    CCCCAGGCTG CTCCTGTTGT CTGCAGACAT GTTCTGGATA CACTCATTCA ATTGGCCAAG
    GTGTTTCCCA GTCACTTCAC ACAGCAGCGG ACCAAAGAAA CAAACTGTGA GAGTGATCGG
    GAGAGGGGCA GTAAGGCCTG CAGCCCATGC TCTTCACAGT CCACAAGCAG TGGCATTTGC
    ACAGACTTCT GGGACTTATT GGTAAAACTG GACAACATGA ATGTCAGCCG GAAAGGCAAG
    AACTCCGTGA AGTCAGTGCC AGTGAGCGCT GGTGGTGAGG GGGAAACCTC CCCATACAGC
    CTCGAAGCTT CTCCACTGGG GCAACTCATG AACATGTTGT CACACCCAGT CATCCGCCGG
    AGCTCTCTCT TAACTGAGAA GCTCCTCAGA CTTCTTTCTC TCATCTCAAT TGCTCTCCCA
    GAAAACAAAG TGTCGGAAGC ACAGGCTAAT TCTGGCAGCA GTGCTTCCTC CACCACTGTT
    GCCACTTCAA CCACATCTAC CACCACTATC AGTGCCACCT CCAGCACATC TACTCTGCCT
    GCTGCAACCA CCCCTGTCAC TTCTGTTCCA GCCCTGGTTG CTGCCACGGC TATATCCACC
    ATTGCCGTAG CCGCTTCAAC CACAGTGACT ACCCCCACAA CTGCTACCAC TACTGTTTCA
    ACTGCTACTA CTACTAAGGC CAGCAAATCT CCAGCTAAAG TTGGTGATAT CGGCAGCAGC
    AGCAGCACAG ACTTTAAGAT GGTATCCTCT GGCCTCACTG AAAATCAGCT CCAGCTCTCT
    GTGGAAGTGT TGACATCCCA TTCTTGTTCT GAAGAAGGCC TAGAAGATGC AGCAAATGTG
    TTACTGCAGC TCTCTCGGGG GGACCCTGGG ACCCGGGACA CTGTTTTGAA GTTGCTACTG
    AATGGAGCCC GCCATCTGGG TTATACGCTT TGTAAACAGA TAGGAACTCT TCTGGCTGAG
    CTACGGGAAT ACAATCTGGA ACAGCAGCGG CGAGCCCAGT GTGAAACCCT CTCCCCTGAT
    GGCATGCCTG AGGAACAGCC ACAGACCACC AAGCTGAAAG GCAAAATGCA GAGCAGGTTT
    GACGTGGCTG AGAATGTGGT AATCGTGGCA TCTCAGAAGC GGCCTCTGGG TGGCCGGGAG
    CTCCAGCTGC CTTCTATGTC CATGTTGACA TCCAAGACAT CTACCCAGAA GTTCTTCTTG
    AGGGTACTGC AGGTCATCAT CCAGCTCCGG GATGACACGC GCCGTGCTAA CAAGAAGGCC
    AAGCAGACAG GCAGGCTAGG TTCCTCCGGT TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT
    GTTCGGCAGC TGGAGGCTGA GGCTGATGCC ATTATACAAA TGGTACGTGA GGGTCAGAGG
    GCGCGGAGAC AGCAACAAGC AGCAACGTCG GAGTCAAGCC AGTCAGAGGC ATCTGTCCGA
    AGGGAGGAGT CACCCATGGA TGTGGACCAG CCATCTCCCA GTGCTCAAGA TACTCAGTCC
    ATTGGCTCAG ATGGAACTCC ACAGGGAGAG AAGGAGAAGG AGGAGAGGCC ACCTGAGTTA
    CCCCTGCTCA GTGAGCAGCT GAACTTGGAT GAGCTGTGGG ACATGCTGGG AGAGTGTCTA
    AAGGAACTGG AGGAATCCCA TGACCAGCAT GCGGTGCTAG TGCTACAACC TGCTGTCGAG
    GCCTTCTTTC TGGTCCATGC AACAGAGCGG GAGAGCAAGC CTCCTGTCCG GGACACCCGT
    GAGAGCCAGC TGGCACACAT CAAGGACGAG CCACCTCCAC TCTCCCCTGC CCCTTTAACC
    CCAGCCACTC CTTCCTCCCT TGACCCGTTC TTCTCCCGGG AGCCCTCATC TATGCACATC
    TCCTCAAGCC TGCCCCCTGA CACACAGAAG TTTCTTCGTT TTGCAGAGAC TCATCGCACT
    GTGTTAAACC AGATCCTACG GCAGTCCACC ACCCACCTCG CTGATGGGCC TTTTGCTGTC
    CTGGTAGACT ACATTCGTGT CCTCGACTTC GATGTCAAGC GCAAATATTT CCGCCAAGAG
    CTGGAGCGTT TGGACGAGGG ACTCCGGAAA GAAGACATGG CTGTGCACGT TCGTCGTGAT
    CATGTCTTTG AAGACTCCTA TCGTGAGCTG CACCGGAAAT CCCCTGAAGA AATGAAGAAT
    CGATTGTATA TAGTATTTGA AGGAGAAGAA GGGCAGGATG CTGGAGGGCT CCTGCGGGAG
    TGGTATATGA TCATCTCTCG AGAGATGTTT AACCCTATGT ATGCCTTGTT CCGTACCTCA
    CCTGGTGATC GGGTCACATA CACCATCAAT CCATCTTCTC ACTGCAACCC CAACCACCTC
    AGCTACTTCA AGTTTGTTGG ACGCATCGTG GCCAAAGCTG TGTATGACAA CCGCCTCCTG
    GAGTGCTACT TTACTAGGTC CTTCTACAAA CACATTTTGG GCAAGTCTGT CAGATATACA
    GATATGGAGA GTGAAGACTA CCACTTTTAC CAGGGCCTGG TTTATCTGCT GGAAAATGAT
    GTCTCCACAC TAGGCTATGA TCTCACTTTC AGCACTGAGG TCCAAGAGTT TGGAGTCTGT
    GAAGTTCGTG ACCTCAAACC CAATGGGGCC AACATCTTGG TAACAGAGGA AAATAAGAAG
    GAATATGTAC ACCTGGTGTG CCAGATGAGA ATGACAGGAG CCATCCGCAA ACAGCTGGCG
    GCTTTCTTAG AAGGTTTCTA TGAGATCATT CCAAAGCGCC TCATTTCCAT CTTCACTGAG
    CAGGAGTTAG AGCTGCTCAT ATCAGGACTG CCCACAATCG ACATTGACGA TTTGAAATCT
    AACACTGAAT ATCACAAGTA CCAGTCCAAC TCTATTCAGA TCCAGTGGTT CTGGAGAGCA
    CTGCGTTCTT TCGATCAAGC TGACCGTGCC AAGTTCCTCC AGTTTGTCAC AGGTACTTCC
    AAGGTGCCCC TGCAAGGTTT TGCTGCCCTT GAAGGCATGA ATGGCATTCA GAAGTTTCAA
    ATCCATCGAG ATGACAGGTC CACAGACCGC CTGCCTTCAG CTCACACATG TTTTAATCAG
    CTGGACCTGC CTGCCTATGA GAGCTTTGAG AAGCTCCGCC ACATGCTACT ATTGGCCATC
    CAGGAGTGCT CTGAAGGCTT TGGGCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48125
    Clone ID Related Accession (Same CDS sequence) XM_010822003.2
    Accession Version XM_010822003.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11655bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
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    601
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    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2221
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    2641
    2701
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    4201
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    9781
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    10201
    10261
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    10381
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    10501
    10561
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    10921
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    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGTCTGCTA GTATTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG 
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820305.1
    CDS16..11670
    Translation

    Target ORF information:

    RefSeq Version XM_010822003.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010822003.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGTCTGCTA GTATTGGAGT CCAGTGTATT CCACAACGAG CCGCACTTCT CAAATCCATG 
    TTGAATTTCC TCAAGAAGGC CATTCAAGAC CCTGCTTTCT CAGATGGCAT ACGACATGTG
    ATGGATGGTT CTCTGCCTAC CTCCCTGAAA CACATCATCA GCAATGCAGA ATACTATGGC
    CCATCACTCT TTCTCCTAGC TACTGAAGTG GTGACTGTGT TTGTATTTCA AGAACCATCA
    TTGCTTTCCT CACTCCAGGA CAATGGATTG ACAGATGTCA TGCTACATGC ACTGCTTATC
    AAAGACGTTC CTGCTACCCG TGAAGTTCTT GGCTCCCTCC CAAATGTTTT CAGTGCACTC
    TGCTTGAATG CCCGAGGTCT GCAGTCATTT GTACAGTGTC AGCCTTTTGA ACGTCTCTTC
    AAAGTTCTCC TGTCTCCAGA TTACCTCCCA GCCATGCGGA GAAGGAGGAG TTCTGATCCT
    CTAGGGGATA CTGCCTCCAA CCTAGGAAGT GCTGTAGATG AGCTCATGAG ACATCAGCCT
    ACCCTCAAAA CAGATGCAAC GACTGCGATC ATCAAGTTAC TTGAAGAAAT CTGTAATCTT
    GGAAGAGACC CTAAATACAT CTGTCAGAAG CCATCAATCC AGAAGGCAGA TGGCACTGCC
    GCTGCTCCTC CCCCAAGATC TAACCATGCT GCAGAAGAGG CCTCTAGTGA GGATGAGGAG
    GAAGAGGAAG TACAGGCCAT GCAGAGCTTT AATTCTACCC AGCAGAATGA AACTGAGCCT
    AATCAGCAGG TTGTTGGTAC AGAGGAGCGT ATTCCTATTC CCCTCATGGA TTACATCCTT
    AATGTGATGA AATTTGTGGA ATCTATTCTG AGCAACAATA CAACTGATGA CCACTGCCAG
    GAATTTGTGA ATCAGAAAGG ACTCTTGCCT TTGGTCACCA TTTTGGGTCT TCCCAATCTG
    CCCATTGACT TTCCCACGTC TGCAGCCTGT CAGGCTGTTG CAGGTGTCTG CAAATCCATA
    TTGACACTGT CACATGAACC CAAAGTCCTC CAGGAAGGTC TCCTTCAGTT GGACTCCATT
    CTTTCCTCCT TGGAGCCCTT ACACCGCCCA ATAGAATCCC CCGGGGGCTC AGTGTTGTTG
    CGAGAACTGG CTTGTGCTGG CAATGTTGCT GATGCTACCC TCTCAGCTCA AGCCACACCT
    CTACTACATG CACTCACTGC TGCCCATGCT TACATCATGA TGTTTGTTCA TACTTGCAGG
    GTTGGACAGA GTGAAATTCG TTCCATCTCC ATAAACCAGT GGGGCTCTCA GTTGGGTCTG
    AGTGTTCTGA GCAAGCTGAG CCAGTTATAC TGTTCCCTGG TATGGGAAAG CACTGTCCTC
    CTCTCCCTCT GTACCCCAAA CAGCTTGCCA TCTGGGTGTG AATTTGGCCA GGCAGACATG
    CAGAAACTGG TTCCAAAGGA TGAGAAGACA GGTACGACCC AGGGCGGAAA AAGATCAGAT
    GGGGATCAGG ATGGAACCAC TGGAAGTATG GATGCTTCTA CCCAGGGCTT GTTGGAAGGC
    ATTGGGCTTG ATGGTGACAC ACTGGCTCCT ATGGAGACAG ACGAACCTAC TGCTTCAGAC
    TCTAAGGGCA AATCTAAAAT CACACCTGCC ATGGCTGCCA GAATCAAGCA AATCAAGCCA
    TTGTTGTCAG CTTCCTCTAG ATTAGGCAGA GCACTGGCTG AGCTGTTTGG ACTCCTTGTT
    AAACTTTGTG TGGGGTCTCC TGTCCGCCAG AGAAGAAGCC ATCATGCTGC CAGCACCACC
    ACAGCACCAA CACCTGCTGC TCGATCAACG GCCTCAGCCC TCACTAAGCT CCTCACAAAA
    GGCTTATCAT GGCAGCCTCC ACCATATACG CCTACTCCAC GCTTCAGGCT GACCTTCTTC
    ATCTGTTCAG TTGGTTTTAC ATCCCCAATG CTGTTTGATG AGAGGAAGTA TCCCTACCAC
    CTCATGCTGC AGAAATTTCT CTGCTCTGGA GGCCACAATG CCCTTTTTGA AACATTCAAC
    TGGGCTCTGT CTATGGGAGG AAAAGTTCCT GTTGCTGAGG GATTAGAACA TTCAGACTTA
    CCTGATGGCA CAGGAGAATT CCTAGATGCC TGGCTAATGC TGGTGGAGAA GATGGTAAAT
    CCCACCACTG TGCTTGAATC TCCACATTCA CTGCCTGCCA AATTGCCTGG AGGTGTTCAG
    AACTTTCCAC AGTTCAGTGC CCTCCGCTTC CTTGTGGTAA CTCAGAAGGC AGCATTTACT
    TGCATCAAGA ACCTGTGGAA CCGGAAACCC TTGAAAGTGT ATGGTGGGCG AATGGCTGAG
    TCCATGCTAG CCATCCTGTG CCACATCCTC CGAGGAGAAC CTGTGATTCG AGAGAGACTG
    AGCAAAGAAA AGGAGGGATC TCGAGGAGAA GAGGATACAG GACAAGAAGA AGGTGGCTCT
    CGCCGGGAAC CTCAAGTCAA CCAGCAACAA CTACAACAGC TCATGGACAT GGGCTTCACA
    AGGGAACATG CCATGGAGGC CCTGTTGAAC ACCAGTACCA TGGAGCAGGC CACAGAGTAC
    CTTCTAACCC ACCCTCCCCC GATCATGGGA GGAGTTGTTC GGGATCTGAG TATGTCTGAA
    GAGGACCAGA TGATGAGAGC GATTGCCATG TCTCTGGGAC AGGATATCCC AATGGATCAA
    AGAGCAGAGT CACCTGAGGA AGTTGCTTGC CGAAAGGAAG AGGAGGAACG GAAAGCTCGA
    GAAAAGCAGG AAGAGGAAGA GGCTAAATGT CTAGAGAAGT TCCAGGATGC TGATCCATTG
    GAGCAAGATG AGCTCCACAC TTTCACAGAT ACAATGTTGC CAGGCTGCTT TCACCTTCTT
    GATGAGTTGC CAGACACTGT ATACCGAGTG TGTGATCTGA TCATGACAGC AATCAAACGA
    AATGGAGCAG ACTATCGAGA CATGATTCTG AAGCAAGTAG TTAATCAGGT GTGGGAAGCT
    GCTGATGTAT TGATCAAAGC TGCTCTTCCC CTGACAACAA GTGACACAAA AACTGTATCA
    GAGTGGATCA GTCAGATGGC CACTCTACCC CAGGCCTCTA ATTTGGCCAC TAGAATCTTG
    CTTTTAACAC TGCTTTTTGA GGAATTGAAG CTACCTTGTG CTTGGGTGGT TGAATCTAGT
    GGCATCCTTA ATGTCCTAAT CAAACTCTTG GAAGTGGTTC AGCCCTGCCT ACAGGCCGCC
    AAGGAACAAA AGGAGGTCCA GACCCCAAAG TGGATCACTC CAGTGTTGCT CCTGATTGAT
    TTCTATGAAA AAACAGCCAT TTCCTCAAAG AGGAGAGCCC AAATGACTAA GTATCTACAG
    TCTAACAACA ACAACTGGCG CTGGTTTGAT GACCGCTCTG GACGTTGGTG TAGTTACAGT
    GCAAGCAACA ACAGCACTAT TGATTCTGCC TGGAAATCTG GAGAGACAAG TGTGCGTTTC
    ACTGCAGGCC GAAGAAGATA CACTGTCCAG TTCACTACAA TGGTACAGGT TAATGAGGAA
    ACAGGGAACC GGCGTCCTGT GATGCTGACA CTCCTGAGGG TACCCCGATT GAATAAAAAT
    TCTAAAAACA GCAATGGACA GGAACTAGAG AAGACACTGG AAGAAAGCAA AGAAATGGAT
    ATCAAACGTA AAGAAAATAA AACCAGTGAT ACCCCCTTGG CCCTAGACAG TACAAATACT
    GAAAAGGAGA CAAGCCTGGA AGAAACAAAA ATTGGGGAGA TCTTGATCCA GGGCCTGACA
    GAGGATATGG TGACTGTTTT AATCCGGGCC TGTGTGAGCA TGCTAGGCGT CCCTGTGGAT
    CCAGACACTT TGCATGCCAC CCTTCGCCTA TGCCTGAGGC TCACCCGAGA CCACAAATAT
    GCCATGATGT TTGCAGAGCT GAAGAGTACT CGCATGATCT TAAATTTGAC CCAGAGTTCA
    GGCTTCAATG GGTTTACTCC CCTGGTTACC CTTCTCTTAA GACACATCAT TGAGGACCCC
    TGCACCCTGC GCCATACCAT GGAAAAGGTT GTTCGCTCAG CAGCTACAAG TGGAGCTGGT
    AGCACTACTT CTGGTGTTGT ATCTGGCAGC CTTGGCTCTC GGGAGATCAA CTACATCCTT
    CGTGTCCTTG GACCAGCCGC ATGCCGCAAC CCAGACATTT TCACAGAAGT GGCCAACTGC
    TGTATCCGCA TTGCCCTTCC AGCCCCTCGC GGCTCAGGAA CTGCTTCAGA TGATGAGTTT
    GAGAATCTCA GAATTAAAGG CCCGAATGCT GTACAGCTGG TGAAAACAAC CCCTTTGAAG
    CCCTCACCTC TGCCTGTCAT TCCTGATACT ATCAAGGAAG TGATCTATGA CATGCTAAAT
    GCCCTGGCTG CATACCATGC TCCAGAGGAA GCAGACAAAT CTGATCCTAA ACCAGGGGGT
    ATGACTCAAG AGGTTGGCCA GCTCCTGCAA GACATGGGTG ATGACGTATA CCAGCAGTAC
    CGGTCACTTA CCCGTCAGAG CAGTGACTTT GATGCACAGA CAGGATTTTC CATTAATAGT
    CAGGTCTTTG CTGCAGATGG TTCCTCCACT GAGACTTCCG CATCTGGGGT ATCCCAAGGA
    GAAGTGTCAA CTCCGGAGGA GTCTCGGGAT GGGAAGAAAG ATAAAGAAGG GGACCGGGCC
    TCTGAGGAAG GCAAGCAGAA AGGCAAGGGC AGCAAACCTT TAATGCCCAC CTCCACTATT
    CTTCGGCTTC TGGCAGAGTT GGTGAGATCC TATGTTGGTA TTGCTACCCT GATTGCCAAC
    TACAGCTACA CTGTGGGCCA GTCTGAGCTG ATCAAAGAGG ACTGCAGTGT GCTAGCTTTT
    GTCTTGGACC ACCTCCTCCC GCATACCCAG AATGCAGAAG ACAAAGACAC ACCGGCGCTG
    GCCCGCCTGT TCCTCGCAAG CCTGGCTGCC TCAGGGAGTG GCACAGATGC CCAGGTGGCC
    CTTGTGAATG AAGTAAAAGC AGCCCTGGGA AGGGCACTGG CTATGGCTGA GAGTACAGAG
    AAACATGCCA GACTTCAGGC AGTGATGTGT ATCATCAGTA CTATCATGGA GTCCTGCCCC
    TCCACCTCCA GTTTCTACAG CAGTGCAACA GCCAAGACCC AACACAACGG CATGAACAAC
    ATCATTCGAC TCTTCCTGAA GAAGGGACTG GTTAATGACC TGGCCAGAGT GCCTCACAGC
    CTGGACCTGT CCAGTCCCAA CATGGCCAAC ACTGTCAATG CTGCTCTGAA GCCTTTGGAA
    ACACTTTCCC GGATTGTGAA CCAGCCCAGT AGCCTTTTTG GCAGTAAGAG TGCTTCTAGC
    AAGAGCAAGT CTGAGCAGGA TGCCCAAGGA GCTGCTCAGG ACTCTAATAG CAACCAACAG
    GACCCAGGCG AGCCTGGGGA AACAGAAGTG CAGGAGGAGG ATCATGATGT CACTCAGACA
    GAGGTGGCAG ATGGGGATAT CATGGATGGG GAGGCTGAAA CCGACTCAGT GGTGATTGCT
    GGGCAGCCTG AGGTGCTCAG TTCACAAGAG ATGCAGGTTG AGAATGAGCT GGAGGACCTG
    ATAGATGAGT TGCTTGAGAG GGATGGCGGA TCTGGGAACA GTACAATTAT AGTGAGCAGA
    AGTGGAGAGG ATGAATCACA AGAGGACGTG CTGATGGATG AAGCTCCTTC CAACCTCAGC
    CAAGCTTCCA CCTTGCAGGC CAACCGAGAA GATTCCATGA ATATCCTGGA CCCTGAGGAT
    GAGGAGGAGC ACACTCAGGA AGAGGACAGC AGTGGCAGTA ACGAGGATGA GGATGATAGT
    CAGGATGAAG AGGAGGAGGA GGAGGAAGAT GAGGAAGATG ATCAGGAGGA TGATGAAGGT
    GAAGAGGGAG ATGAAGACGA TGACGACGAT GGCTCTGAAA TGGAATTGGA TGAGGATTAC
    CCTGATATGA ACGCTTCTCC CTTGGTCCGA TTTGAGCGCT TTGACCGGGA GGATGATCTC
    ATCATTGAGT TTGACAACAT GTTCTCCAGT GCTACAGACA TCCCCCCATC CCCAGGAAAT
    ATCCCTACCA CCCACCCACT GATGGTACGC CATGCAGATC ACAGTTCCCT GACACTGGGC
    AGTGGCTCCT CTACAACACG TCTCACCCAG GGCATTGGGC GCAGTCAAAG GACCCTGAGA
    CAGCTGACAG CCAACACAGG CCATACCATT CACGTTCACT ACCCTGGGAA TCGCCAGCCC
    AATCCTCCTC TTATACTCCA GAGGTTGCTT GGTCCCTCTG CTGCTGCTGA CATCCTTCAG
    CTGAGCAGCA GCCTTCCCCT ACAAAGCCGA GGCCGAGCCC GCCTCCTAGT GGGCAACGAT
    GACGTCCACA TCATCGCCCG GTCTGATGAT GAACTACTGG ATGACTTTTT CCATGATCAG
    AGCACAGCTA CCAGCCAAGC AGGGACCCTA TCCAGTATCC CTACAGCCCT GACTCGCTGG
    ACAGAGGAAT GCAAAGTTCT TGATGCTGAG AGCATGCATG ACTGTGTTTC AGTTGTTAAA
    GTGCCCATTG TCAATCATCT GGAATTCCTG AGGGATGAGG AGCTGGAAGA AAGGAGGGAG
    AAAAGGAGGA AACAACTAGC TGAGGAAGAA ACAAAGATAA CCGACAAAGG CAAAGAAGAT
    AAGGAGAACA GGGATCAGAG TGCACAGTGT GCTGCATCTA AGACAAATGA CTCCACTGAA
    CAGAACCTCT CAGATGGGAC TCCTATGCCT GACAGCTACC CAGCAACCCC ATCTTCAACT
    GATGTAGCTA CATCTGAGCC CAGGGAGACC CTTGTCACTC TGCAGCCCTC CCAACAGCAA
    CCAACACTCC CAACCCCACC AGCCTTGGGA GAGATTCCTC AAGAGCTGCA GTCCCCAATT
    GGAGAAGGTG GGAGCTCTAC ACAGCTATTG ATGCCTGTAG AGCCAGAGGA ATTGGGTCCC
    ACAAGGCCAA GTGGAGAAGC AGAAACAACT CAGATGGAGC TATCTCCAGC TCCCACTATA
    ACCTCTCTTT CCCCAGAGAG AGCTGAAGAT TCTGATGCTC TGACAGCTGT CAGCAGTCAA
    CTGGAAGGCT CTCCCATGGA TACTAGCAGC CTGGCTTCCT GTACCCTGGA GGAGGCTGTG
    GGTGACGCCC CAGCAACTGG CAGTTCTGAG CAGCCCACAG CAGGCAGCTC CACTCCTGGG
    GATGCCCCAC CAGTTGTGAC AGAAGTACAA GGCAGGGGTG ATGGGTCAGG GGAACCTACC
    CAGCCCCCTG AGGACAGCTC TCCCCCTGCA TCCTCTGAGA GTTCTTCCAC CAGAGATTCC
    GCGGTGGCCA TTTCTGGAGC AGATTCCCGG GGAATCCTGG AAGAGCCATT GCCTTCAACA
    AGCAGTGAAG AAGAAGATCC TCTTGCAGGT ATTAGTCTCC CCGAAGGTGT GGACCCCTCT
    TTTCTGGCCG CTCTGCCTGA TGACATTCGC CGAGAAGTCC TACAGAACCA GCTGGGCATT
    CGTCCACCAA CACGAACTGC CCCTTCAACA AATAACTCAG CTCCTGCAGT GGTGGGGAAT
    CCTGGTGTCA CCGAAGTGAG CCCTGAGTTC CTGGCTGCTC TGCCTCCAGC CATCCAGGAG
    GAAGTACTGG CACAGCAGAG AGCTGAGCAA CAGCGACGGG AACTAGCACA AAATGCCAGC
    TCAGACACCC CCATGGACCC TGTGACCTTC ATCCAGACTC TGCCCTCAGA CCTGCGCCGT
    AGTGTCCTAG AGGACATGGA GGACAGTGTG TTGGCCGTGA TGCCACCTGA CATCGCAGCT
    GAAGCTCAAG CCCTGAGGCG AGAGCAAGAA GCCCGGCAGA GGCAGCTCAT GCATGAGCGA
    CTTTTTGGGC ACAGCAGCAC CTCTGCACTC TCTGCCATTC TCCGAAGCCC AGCCTTCACC
    AGTCGCCTGA GTGGCAACCG TGGGGTCCAA TACACTCGCC TTGCTGTGCA AAGAGGTGGC
    ACCTTCCAGA TGGGGGGTAG CAGCAGCCAT AATAGGCCTT CTGGCAGTAA TGTGGACACC
    CTCCTACGTC TTCGAGGACG GCTCCTCCTG GATCACGAAG CCCTGTCTTG TCTCCTGGTC
    CTACTTTTTG TGGATGAGCC AAAACTCAAT ACTAGCCGTC TTCACCGAGT ACTGAGAAAT
    CTCTGCTACC ATGCCCAGAC CCGCCACTGG GTTATCCGTA GCCTGCTTTC CATCTTGCAG
    CGAAGCAGTG AGAGTGAGCT TTGCATTGAA ACACCCAAGC TCTCCTCAAG TGAGGAAAAG
    GGCAAAAAGT CAAGCAAGAG CTGTGGGTCA AGCAGCCATG AGAACCGGCC CCTGGACCTG
    CTACACAAGA TGGAGTCTAA GAGTTCCAAC CAGCTTTCCT GGCTCTCAGT ATCCATGGAT
    GCAGCCCTAG GCTGCAGGAC TAATATATTC CAGATCCAGC GTTCAGGGGG TCGCAAACAT
    ACTGAGAAAC ATGCGAGTAG TGGCTCCACT GTCCATATCC ACCCCCAGGC TGCTCCTGTT
    GTCTGCAGAC ATGTTCTGGA TACACTCATT CAATTGGCCA AGGTGTTTCC CAGTCACTTC
    ACACAGCAGC GGACCAAAGA AACAAACTGT GAGAGTGATC GGGAGAGGGG CAGTAAGGCC
    TGCAGCCCAT GCTCTTCACA GTCCACAAGC AGTGGCATTT GCACAGACTT CTGGGACTTA
    TTGGTAAAAC TGGACAACAT GAATGTCAGC CGGAAAGGCA AGAACTCCGT GAAGTCAGTG
    CCAGTGAGCG CTGGTGGTGA GGGGGAAACC TCCCCATACA GCCTCGAAGC TTCTCCACTG
    GGGCAACTCA TGAACATGTT GTCACACCCA GTCATCCGCC GGAGCTCTCT CTTAACTGAG
    AAGCTCCTCA GACTTCTTTC TCTCATCTCA ATTGCTCTCC CAGAAAACAA AGTGTCGGAA
    GCACAGGCTA ATTCTGGCAG CAGTGCTTCC TCCACCACTG TTGCCACTTC AACCACATCT
    ACCACCACTA TCAGTGCCAC CTCCAGCACA TCTACTCTGC CTGCTGCAAC CACCCCTGTC
    ACTTCTGTTC CAGCCCTGGT TGCTGCCACG GCTATATCCA CCATTGCCGT AGCCGCTTCA
    ACCACAGTGA CTACCCCCAC AACTGCTACC ACTACTGTTT CAACTGCTAC TACTACTAAG
    GCCAGCAAAT CTCCAGCTAA AGTTGGTGAT ATCGGCAGCA GCAGCAGCAC AGACTTTAAG
    ATGGTATCCT CTGGCCTCAC TGAAAATCAG CTCCAGCTCT CTGTGGAAGT GTTGACATCC
    CATTCTTGTT CTGAAGAAGG CCTAGAAGAT GCAGCAAATG TGTTACTGCA GCTCTCTCGG
    GGGGACCCTG GGACCCGGGA CACTGTTTTG AAGTTGCTAC TGAATGGAGC CCGCCATCTG
    GGTTATACGC TTTGTAAACA GATAGGAACT CTTCTGGCTG AGCTACGGGA ATACAATCTG
    GAACAGCAGC GGCGAGCCCA GTGTGAAACC CTCTCCCCTG ATGGCATGCC TGAGGAACAG
    CCACAGACCA CCAAGCTGAA AGGCAAAATG CAGAGCAGGT TTGACGTGGC TGAGAATGTG
    GTAATCGTGG CATCTCAGAA GCGGCCTCTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG
    TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT GCAGGTCATC
    ATCCAGCTCC GGGATGACAC GCGCCGTGCT AACAAGAAGG CCAAGCAGAC AGGCAGGCTA
    GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAGA GGGCGCGGAG ACAGCAACAA
    GCAGCAACGT CGGAGTCAAG CCAGTCAGAG GCATCTGTCC GAAGGGAGGA GTCACCCATG
    GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAGT CCATTGGCTC AGATGGAACT
    CCACAGGGAG AGAAGGAGAA GGAGGAGAGG CCACCTGAGT TACCCCTGCT CAGTGAGCAG
    CTGAACTTGG ATGAGCTGTG GGACATGCTG GGAGAGTGTC TAAAGGAACT GGAGGAATCC
    CATGACCAGC ATGCGGTGCT AGTGCTACAA CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT
    GCAACAGAGC GGGAGAGCAA GCCTCCTGTC CGGGACACCC GTGAGAGCCA GCTGGCACAC
    ATCAAGGACG AGCCACCTCC ACTCTCCCCT GCCCCTTTAA CCCCAGCCAC TCCTTCCTCC
    CTTGACCCGT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT
    GACACACAGA AGTTTCTTCG TTTTGCAGAG ACTCATCGCA CTGTGTTAAA CCAGATCCTA
    CGGCAGTCCA CCACCCACCT CGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT
    GTCCTCGACT TCGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTGGACGAG
    GGACTCCGGA AAGAAGACAT GGCTGTGCAC GTTCGTCGTG ATCATGTCTT TGAAGACTCC
    TATCGTGAGC TGCACCGGAA ATCCCCTGAA GAAATGAAGA ATCGATTGTA TATAGTATTT
    GAAGGAGAAG AAGGGCAGGA TGCTGGAGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT
    CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGGGTCACA
    TACACCATCA ATCCATCTTC TCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTT
    GGACGCATCG TGGCCAAAGC TGTGTATGAC AACCGCCTCC TGGAGTGCTA CTTTACTAGG
    TCCTTCTACA AACACATTTT GGGCAAGTCT GTCAGATATA CAGATATGGA GAGTGAAGAC
    TACCACTTTT ACCAGGGCCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT
    GATCTCACTT TCAGCACTGA GGTCCAAGAG TTTGGAGTCT GTGAAGTTCG TGACCTCAAA
    CCCAATGGGG CCAACATCTT GGTAACAGAG GAAAATAAGA AGGAATATGT ACACCTGGTG
    TGCCAGATGA GAATGACAGG AGCCATCCGC AAACAGCTGG CGGCTTTCTT AGAAGGTTTC
    TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTC
    ATATCAGGAC TGCCCACAAT CGACATTGAC GATTTGAAAT CTAACACTGA ATATCACAAG
    TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CACTGCGTTC TTTCGATCAA
    GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTGCC CCTGCAAGGT
    TTTGCTGCCC TTGAAGGCAT GAATGGCATT CAGAAGTTTC AAATCCATCG AGATGACAGG
    TCCACAGACC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGACCT GCCTGCCTAT
    GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTATTGGCCA TCCAGGAGTG CTCTGAAGGC
    TTTGGGCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.