huwe1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following huwe1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the huwe1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa46263 XM_018096571.1
Latest version!
Xenopus tropicalis HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (huwe1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa65588 XM_031892168.1
Latest version!
Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa65589 XM_031892169.1
Latest version!
Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa65590 XM_031892170.1
Latest version!
Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa65591 XM_031892171.1
Latest version!
Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa65592 XM_031892172.1
Latest version!
Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa65593 XM_031892173.1
Latest version!
Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa65594 XM_018096571.2
Latest version!
Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa65595 XM_031892175.1
Latest version!
Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa65596 XM_031892176.1
Latest version!
Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa65597 XM_031892177.1
Latest version!
Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa46263
    Clone ID Related Accession (Same CDS sequence) XM_018096571.1
    Accession Version XM_018096571.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HUWE1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    481
    541
    601
    661
    721
    781
    841
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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
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    3601
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    3901
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    4021
    4081
    4141
    4201
    4261
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    4441
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    4561
    4621
    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
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    8161
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    8281
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    8401
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    8521
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    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
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    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGGTGCTG ATGGATATGG GTTTCACAAG GGAGCATGCT
    CTGGAAGCTC TGCTTAACAC GAGTACTATG GAGCAGGCCA CTGAATATCT CCTCACCCAC
    CCTCCACCTC TTATTGGCGG AGTTGTACGG GACTTAAGTA TGTCAGAGGA AGATCAGATG
    CTGAGAGCCA TTGCCATGTC GCTGGGACAA GATATCCCTA TGGAACAGAG GGCAGAACTG
    CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG GAGGAACGCA AGGCACGAGA AAAACAAGAG
    GAAGAGGAAG CTAAATGCTT GGAAAAGTTT CAAGATGCAG AACCCCTGGA ACAGGATGAG
    CTTCAAGGAT TCACAGACAC CATGCTCCCT GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA
    GACACAGTCT ATAGAGTGTG TGATCTTATA ATGACTGCCA TAAAGCGCAA TGGGGCAGAC
    TATCGAGACA TGATTCTAAA ACAAGTGGTT AACCAGGTAT GGGAAGCCGC TGATGTTCTT
    ATTAAAGCTG CACTGCCGCT GACCACAAGT GACACAAAAA CTGTGTCTGA GTGGATCAGC
    CAAATGGCTA CTCTTCCTCA AGCTTCTAAC TTGGCCACTC GCATCCTTTT GCTCACACTG
    CTGTTTGAGG AGCTAAAACT GCCATGTGCT AGAGTTGTTG AAACAAGTGG AATACTTAAC
    GTACTAATTA AGCTGCTTGA AGTGGTTCAG CCATGTTTGC AGGCAGCCAA GGAGCAAAAG
    GAAGTTCAGA CACCAAAGTG GATTACACCA GTCCTCCTCT TAATAGATTT TTATGAGAAG
    ACCTCAATTT CTTCTAAAAG AAGAATGCAA ATGAACAGGT ACCTTCAAGC TAATGGAAAC
    AACTGGCGAT GGTTTGATGA TCGGTCTGGC CGTTGGTGCA GCTACAGTGC TAGTAACAAC
    AGCACTATTG ATGCAGCCTG GAAAGCAGGA GAAACATGTG TGCGATTTAC AGCTGGCCGT
    CGAAGATATA CTGTGCAGTT TACCACAATG GTTCAGGTAA ATGAAGAGAC CGGCAATAGA
    CGACCTGTCA TGCTGACTCT CTTGCGTGTT CCGAGACTTT TAAAGAGTGG AAAAAATGGA
    AATGGACAGG AACTTGAAAA GACACTTGAG GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA
    GACGAAAAAG TGCCTGAGCC CATTCCAGTT CCTGAAACCT CTTCCACTGA GAAAGATGCA
    CCAACTGAAG AGCCCAAAGT TGGAGACATA GTAATTCAGG GTCTGGCTGA AGAAATGGTG
    ACCGTCCTTA TACGTTCCTG CGTCGGCATG CTTGGTGTTC CTGTTGATCC AGATACTCTT
    CATGCAACCT TGCGTCTTTG CCTGCGTCTC ACACGTGACC ACAAATATGC CATGATGTTT
    GCAGAGCTTA AGAGCACTCG AATGATCCTC AGCTTAACTC AAAGCTCTGG ATTCAATGGC
    TTTACTCCCC TTGTGACGCT CCTATTTAGG CACATCATAG AGGATCCTTG CACCCTCCAG
    CACACCATGG AAAAGGTTGT ACGGTCTGCA GCAACTAGTG GGGCAGGGAG TACCACATCT
    GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT GAAATCAACT ATATACTTAG AGTACTGGGT
    CCAGCAGCTT GTCGAAACCC TGACATTTTC ACTGAAGTGG CAAACAGTTG CATTCGCATT
    GCTCTCCCTG CACCTCGTGG ATCTGGAACT GCCTCTGATG ATGAGTTTGA GAACCTTCGG
    ATCAAAGGTC CTAATGCAGT TCAGTTGGTT AAAACCACCC CTATAAAGCC CTCCACCTTG
    CCAGTCATCC CTGATACAAT TAAGGAAGTA ATCTATGACA TGCTGAATGC ACTGGCAGCT
    TACCACGCCC CTGAGGAAGT ATCTGAAAAG TCTGAGCAAA AACCTGTTGG CATGAACCAG
    GAAGTCAGCC AGATCCTACA GGACATGGGT GATGAGGTTT TCCAGCAATA TCGGGACTTA
    ACCAGACAGG GAAGCGACTT TGATGGTCAG ACCAGTTTTG CCATCAACGC GCGAGTCTTT
    ACTGCTGACA GCACAGCTGC CGAAACACCT CAGTCTGGTA CACCTCAAGG TGAAACTGCC
    TTAACACCTG AAGAAAACCG AGAAGGGTTA AAGAAAGATA AGGAAGGAGA CCGTAGTTCA
    GAAGATGGAA AGCAGAAGGC AAAAGGTAGT AAGCCTTTGC TGCCTACCTC TTCCATTCTT
    CGACTACTTG CTGAACTTGT CAGGTCCTAT GTCGGAATAG CCTCGCTGAT CGCCACCTAC
    AATTACACTG CAGGACAATC TGAACTAATT AAAGAGGATT GCAGTGTATT GGCGTTTGTG
    CTGGATCACC TGCTACCCCA CACACATAAT GCAGAGGACA AGGACACACC AGCCCTAGCC
    CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA GGAAGTGGCA CTGATGCCCA GGTTGCACTA
    GTGAATGAAG TAAAGGCAGC TCTTGGCAGA GCCCTAGCAA TGGCTGAGAG CACGGAGAAG
    CATGCTAGGT TGCAGGCTGT GATGTGCATT ATCAGTACCA TTATGGAGTC ATGTCCATCC
    ACCTCCAGCT TCTACAGCAC AGCAACCACC AAAACCCAGC ACAATGGCAT GAACAACATC
    ATCAGACTCT TTTTGAAAAA GGGGCTGGTT AGTGATTTAG CCAGGGTCCC TCACAGCCTT
    GATTTATCCA GTCCAAATAT GGCAAACACC GTGAATGCAG CACTGAAGCC ACTCGAGACA
    CTGTCTCGCA TTGTCAATCA ACCCAGCAGT TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG
    AATAAATCTG AGGCAGATAC ACATGGAACA GCCCAGGATC CCAACAGTAA TCAGCCAGAT
    ACAGGTGAAA CAGGAACAGA AGTGCAGGAA GAGGACCCAG ATGCTTCTCA TGCTGAAGTA
    GCTGATGGAG ACATCATGGA TGGAGAGACA GAGACTGATG CTGTGGTTAT TGCTGGCCAG
    CCTGAAGTCC TTACTACACA AGAAATGCAG GTAGAGAATG AGCTTGAAGA TCTGATAGAT
    GAACTTCTTG AAAGGGATGG TGCTGCAGGT AACCGGGTCG GAGAAGATGA ATCACAGGAA
    GATGTGCTGA TGGATGAGCC TCCTACTAAC CTCAGCCAAG CCTCCACTCT TCAGGCTAAT
    CGAGAAGACT CTATGAACAT CTTAGACCCA GAGGATGAGG AAGAGCACAC CCAAGAAGAG
    GACAGCAGTG GCAGCAATGA AGATGAAGAT GACAGCCAGG AAGAGGAGGA GGAAGAGGAA
    GATGAGGACG ATCAGGATGA TGAGGAGGGT GAGGAAGGGG ATGAGGATGA TGATGATGAC
    TGTTCAGAGA TGGAACTGGA TGAGGATTAT CCAGACATGA ATGCCTCACC TCTTGTACGC
    TTTGAACGTT TTGACAGAGA AGATGATTTA ATCATAGAGT TTGATAACAT GTTCTCCAGT
    GCCACTGAGA TCCCTCCATC CCCTGGTAAC ATCCCTACTA CACACCCCCT AATGGTGCGA
    CACGCTGATC ACGGCTCCTT GACTCTTGGC AGTGGCACTT CCACTACTCG CCTGGCACAG
    GGCATCGGGC GCAGCCAGAG AACACTCCGA CAACTCACAG CCAACACCGG TCACACAATA
    CATGTTCATT ATCCTGGCAA CAGGCAGCCA AATCCACCTT TAATCCTCCA GAGGTTGCTG
    GGGCCCTCAG CCGCTGCTGA CATCTTGCAA CTAAGCAGCA GTCTTCCTCT GCAGAGTCGT
    GGCCGAGCAC GTCTACTTGT TGGCAACGAT GATGTCCACA TCATAGCACG CTCTGATGAT
    GAGATGCTTG ATGATTTTTT CCATGATCAG AGTACAGCCA CAAGTCAGGC TGGCACACTT
    TCCAGCATTC CAACTGCGCT GACCCGCTGG ACAGAAGAGT GTAAGGTCTT GGATGCTGAA
    AGCATGCATG ACTGTGTATC TGTGGTCAAA GTCCCAATCA TCAATCATTT GGAATCTTTG
    CGTGATGAGG AGCTTGAGGA GCGCAGGGAA AAAAGAAGGA AACAACTAGC AGAAGAGGAG
    GCCAAGATGG CAGAGAAATC CAAGGAGGAG AAGGAAGCAA AGGAGCAAAA TTCACAGGGT
    GCCAATTCAA AGCAGAATAC CCCATTTGAA CTGAGCCTTG CAGATCAAGA TGGAAGTGGT
    CGTCCTGAAA ATTTCCCAAC TGCACCATCT TCTGCAGAAG GAACTCCAAC AAGTCCTGAA
    ATTGTTCGTT TAGATCCCAC CCTAAGGGCT ACTGAGGCTA CTCTGGCAGA GCTGACTCAG
    GGTCTGCCTG CTGATACAGG CACGGAGGAT GTTGGGCAAC AACTCATGCC ATCTCACCAA
    GAGGACTCTG GACCAGGACG GCCAAGTGTA GAGGGGGATG CCACACAAAT GGAGCTATCG
    CCAGCACCCA CTATAACCTC CTTATCACCA GAGCGAGCAG AGGATTCAGA TGCTTTGACA
    GCTGTTAGTA GCCAACTAGA GGGCTCGCCT ATGGACACCA GCAGTCTTGC CTCTGGGCCC
    TTAGAGGATG GCGTAGGGGA GGCACCTGGA ATTGTTAATC CAGAGCAGCC TCTTGTGGTT
    GGCAGTAGCA TTGCTGTTGG ACAGCAACCA GTGGAACCAC CGACGACCTG TGAAACCCAA
    CCAGTAGTTT CTCAACACAG TGATAGCTCA CAAATTGCCC CTTCCTCTGA GTTGCCTTCC
    ACAAGAGAAA GTGCTGTGAC CTTGTTGGCT ACAGATTCTA GTGGTATCCT TGAAGAACCT
    TTGCCTTCTA CAAGCAGTGA AGAGGAAGAC CCATTAGCAG GCATAAATCT TCCAGAGGGT
    GTGGATCCTT CTTTCCTAGC GGCTCTTCCT GAAGACATCC GCAGAGAGGT GCTGCAGAAT
    CAGTTGGGCA TACGGCCGCC ATCGCGACCT CCACCCACTG CTGCCCCAGT TATTCCTCCT
    CCTGTGGTTG CTAATGCTGG TGTCACGGAG GTCAGCCCTG AGTTCTTGGC AGCGTTGCCA
    CCAGCCATTC AGGAAGAGGT CCTAGCACAG CAGCGAGCAG AACAGCAGAG GCGGGAACTT
    GCTCAGACCA CTACGTCTGA TACACCCATG GATCCTGTGA CTTTCATTCA GACGTTACCT
    TCTGATCTAC GTCGTAGTGT GTTAGAAGAC ATGGAGGACA GTGTTTTAGC TGTGATGCCA
    CCTGACATTG CTGCAGAGGC ACAGGCTCTA AGGCGAGAGC AAGAAGCTCG GCAGAGGCAA
    CTAATGCATG AACGACTGTT CGGGCACAGT AGCACATCTG CACTCTCTGC CATCCTTAGG
    AGTCCTGCTT TCACGAGTCG CTTGAGTGGA AACCGAGGTG TTCAGTACAC ACGGTTGGCT
    GTACAGAGAG GGAACACTTT CCAAATGGGA AGCAGCAGTA CACATACCAG ACCAACCGGT
    AGTAATGTTG ACAGCCTACT ACGATTACGA GGCCGACTAT TACTTGATCA TGAAGCCCTC
    TCCTGTCTGC TTGTCCTACT GTTTGTTGAT GAACCCAAGC TAAACACCAG TCGCTTGCAC
    CGTGTTCTAC GCAACCTCTG CTACCATGCA CAAACAAGGA ACTGGGTGAT TAGAAGCTTG
    TTGTCTATAC TGCAGCGTAG CAGTGAAAGT GAGCTTTGTA TAGAAAGCCC AAAGACTTCT
    ATTGGGGGCA CTGATAAACG GGGTGCAAAA ACAAGTCATG ACAACCGCCC TTTAGAATTT
    CTGCATAAGA TGGAATCGAA GAATTCCAAC CAGCTCTCCT GGCTTTCTGT ATCCATGGAC
    GCTGCTCTTG GCTGCCGTAC GAACATCTTC CAAATCCAGA GGTCCCTAGG ACGCAAGCAT
    ACAGAAAAGC ATTCAGCCTG TGGTTCTACT GTCCACATCC ATCCCCAAGC AGCCCCTGTA
    GTGTGTAGAC ATGTGCTTGA CACACTAATA CAGCTAGCTA AGGTGTTTCC CAGTCATTTC
    ACACAGCAGC GAACCAAAGA ATGTAGTGTG GAAAACAGTG ACAGCAGGGA GCGGAGTATA
    AAACTTACTT GTAGTCCGTG TCTTGCACCA TCTAGTAGTA TTGGAGCAAG CAACATCTCC
    ACAGATTTCT GGGACTTGCT AGTCAAGCTA GACAACATGA ATGTGAGCCG GAAGGGGAAA
    ACGTCTATAA AATCTGTGCC AGTGAGCTCT GGTTCTGAAG GCGAGAGCAA TCAGTGCAGC
    CTGGAGGCCT CCCCCTTAGG ACAGCTTATG AATATGCTGT CTCATTCTGT TATTCGCCGC
    AGTTCGCTGC TTACTGAAAA GCTGCTGAGA CTTCTCTCCC TTATCTCTAT TGCCCTTCCT
    GATAATAAGG CTTCTGAAAC AGCAGGCAAT CAGAGCAATT CTATTACCCC AGCACCTGCA
    CCTACGCCTG CAACTGCACC AGCCCCTGTG CCTGTACCTG CTGTAACAGT TCCAGTTGTT
    GTGAGCACAA ACATAGTGAC AACTGTTACA ACCAAAAGTG GAAAATCTCC TGCAAAATCA
    AGTGAAGTCA GCAATGCACC AGATAAGATG GCAGCTTCTG GCCTTACATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952060.1
    CDS694..11424
    Translation

    Target ORF information:

    RefSeq Version XM_018096571.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (huwe1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096571.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGGTGCTG ATGGATATGG GTTTCACAAG GGAGCATGCT
    CTGGAAGCTC TGCTTAACAC GAGTACTATG GAGCAGGCCA CTGAATATCT CCTCACCCAC
    CCTCCACCTC TTATTGGCGG AGTTGTACGG GACTTAAGTA TGTCAGAGGA AGATCAGATG
    CTGAGAGCCA TTGCCATGTC GCTGGGACAA GATATCCCTA TGGAACAGAG GGCAGAACTG
    CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG GAGGAACGCA AGGCACGAGA AAAACAAGAG
    GAAGAGGAAG CTAAATGCTT GGAAAAGTTT CAAGATGCAG AACCCCTGGA ACAGGATGAG
    CTTCAAGGAT TCACAGACAC CATGCTCCCT GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA
    GACACAGTCT ATAGAGTGTG TGATCTTATA ATGACTGCCA TAAAGCGCAA TGGGGCAGAC
    TATCGAGACA TGATTCTAAA ACAAGTGGTT AACCAGGTAT GGGAAGCCGC TGATGTTCTT
    ATTAAAGCTG CACTGCCGCT GACCACAAGT GACACAAAAA CTGTGTCTGA GTGGATCAGC
    CAAATGGCTA CTCTTCCTCA AGCTTCTAAC TTGGCCACTC GCATCCTTTT GCTCACACTG
    CTGTTTGAGG AGCTAAAACT GCCATGTGCT AGAGTTGTTG AAACAAGTGG AATACTTAAC
    GTACTAATTA AGCTGCTTGA AGTGGTTCAG CCATGTTTGC AGGCAGCCAA GGAGCAAAAG
    GAAGTTCAGA CACCAAAGTG GATTACACCA GTCCTCCTCT TAATAGATTT TTATGAGAAG
    ACCTCAATTT CTTCTAAAAG AAGAATGCAA ATGAACAGGT ACCTTCAAGC TAATGGAAAC
    AACTGGCGAT GGTTTGATGA TCGGTCTGGC CGTTGGTGCA GCTACAGTGC TAGTAACAAC
    AGCACTATTG ATGCAGCCTG GAAAGCAGGA GAAACATGTG TGCGATTTAC AGCTGGCCGT
    CGAAGATATA CTGTGCAGTT TACCACAATG GTTCAGGTAA ATGAAGAGAC CGGCAATAGA
    CGACCTGTCA TGCTGACTCT CTTGCGTGTT CCGAGACTTT TAAAGAGTGG AAAAAATGGA
    AATGGACAGG AACTTGAAAA GACACTTGAG GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA
    GACGAAAAAG TGCCTGAGCC CATTCCAGTT CCTGAAACCT CTTCCACTGA GAAAGATGCA
    CCAACTGAAG AGCCCAAAGT TGGAGACATA GTAATTCAGG GTCTGGCTGA AGAAATGGTG
    ACCGTCCTTA TACGTTCCTG CGTCGGCATG CTTGGTGTTC CTGTTGATCC AGATACTCTT
    CATGCAACCT TGCGTCTTTG CCTGCGTCTC ACACGTGACC ACAAATATGC CATGATGTTT
    GCAGAGCTTA AGAGCACTCG AATGATCCTC AGCTTAACTC AAAGCTCTGG ATTCAATGGC
    TTTACTCCCC TTGTGACGCT CCTATTTAGG CACATCATAG AGGATCCTTG CACCCTCCAG
    CACACCATGG AAAAGGTTGT ACGGTCTGCA GCAACTAGTG GGGCAGGGAG TACCACATCT
    GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT GAAATCAACT ATATACTTAG AGTACTGGGT
    CCAGCAGCTT GTCGAAACCC TGACATTTTC ACTGAAGTGG CAAACAGTTG CATTCGCATT
    GCTCTCCCTG CACCTCGTGG ATCTGGAACT GCCTCTGATG ATGAGTTTGA GAACCTTCGG
    ATCAAAGGTC CTAATGCAGT TCAGTTGGTT AAAACCACCC CTATAAAGCC CTCCACCTTG
    CCAGTCATCC CTGATACAAT TAAGGAAGTA ATCTATGACA TGCTGAATGC ACTGGCAGCT
    TACCACGCCC CTGAGGAAGT ATCTGAAAAG TCTGAGCAAA AACCTGTTGG CATGAACCAG
    GAAGTCAGCC AGATCCTACA GGACATGGGT GATGAGGTTT TCCAGCAATA TCGGGACTTA
    ACCAGACAGG GAAGCGACTT TGATGGTCAG ACCAGTTTTG CCATCAACGC GCGAGTCTTT
    ACTGCTGACA GCACAGCTGC CGAAACACCT CAGTCTGGTA CACCTCAAGG TGAAACTGCC
    TTAACACCTG AAGAAAACCG AGAAGGGTTA AAGAAAGATA AGGAAGGAGA CCGTAGTTCA
    GAAGATGGAA AGCAGAAGGC AAAAGGTAGT AAGCCTTTGC TGCCTACCTC TTCCATTCTT
    CGACTACTTG CTGAACTTGT CAGGTCCTAT GTCGGAATAG CCTCGCTGAT CGCCACCTAC
    AATTACACTG CAGGACAATC TGAACTAATT AAAGAGGATT GCAGTGTATT GGCGTTTGTG
    CTGGATCACC TGCTACCCCA CACACATAAT GCAGAGGACA AGGACACACC AGCCCTAGCC
    CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA GGAAGTGGCA CTGATGCCCA GGTTGCACTA
    GTGAATGAAG TAAAGGCAGC TCTTGGCAGA GCCCTAGCAA TGGCTGAGAG CACGGAGAAG
    CATGCTAGGT TGCAGGCTGT GATGTGCATT ATCAGTACCA TTATGGAGTC ATGTCCATCC
    ACCTCCAGCT TCTACAGCAC AGCAACCACC AAAACCCAGC ACAATGGCAT GAACAACATC
    ATCAGACTCT TTTTGAAAAA GGGGCTGGTT AGTGATTTAG CCAGGGTCCC TCACAGCCTT
    GATTTATCCA GTCCAAATAT GGCAAACACC GTGAATGCAG CACTGAAGCC ACTCGAGACA
    CTGTCTCGCA TTGTCAATCA ACCCAGCAGT TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG
    AATAAATCTG AGGCAGATAC ACATGGAACA GCCCAGGATC CCAACAGTAA TCAGCCAGAT
    ACAGGTGAAA CAGGAACAGA AGTGCAGGAA GAGGACCCAG ATGCTTCTCA TGCTGAAGTA
    GCTGATGGAG ACATCATGGA TGGAGAGACA GAGACTGATG CTGTGGTTAT TGCTGGCCAG
    CCTGAAGTCC TTACTACACA AGAAATGCAG GTAGAGAATG AGCTTGAAGA TCTGATAGAT
    GAACTTCTTG AAAGGGATGG TGCTGCAGGT AACCGGGTCG GAGAAGATGA ATCACAGGAA
    GATGTGCTGA TGGATGAGCC TCCTACTAAC CTCAGCCAAG CCTCCACTCT TCAGGCTAAT
    CGAGAAGACT CTATGAACAT CTTAGACCCA GAGGATGAGG AAGAGCACAC CCAAGAAGAG
    GACAGCAGTG GCAGCAATGA AGATGAAGAT GACAGCCAGG AAGAGGAGGA GGAAGAGGAA
    GATGAGGACG ATCAGGATGA TGAGGAGGGT GAGGAAGGGG ATGAGGATGA TGATGATGAC
    TGTTCAGAGA TGGAACTGGA TGAGGATTAT CCAGACATGA ATGCCTCACC TCTTGTACGC
    TTTGAACGTT TTGACAGAGA AGATGATTTA ATCATAGAGT TTGATAACAT GTTCTCCAGT
    GCCACTGAGA TCCCTCCATC CCCTGGTAAC ATCCCTACTA CACACCCCCT AATGGTGCGA
    CACGCTGATC ACGGCTCCTT GACTCTTGGC AGTGGCACTT CCACTACTCG CCTGGCACAG
    GGCATCGGGC GCAGCCAGAG AACACTCCGA CAACTCACAG CCAACACCGG TCACACAATA
    CATGTTCATT ATCCTGGCAA CAGGCAGCCA AATCCACCTT TAATCCTCCA GAGGTTGCTG
    GGGCCCTCAG CCGCTGCTGA CATCTTGCAA CTAAGCAGCA GTCTTCCTCT GCAGAGTCGT
    GGCCGAGCAC GTCTACTTGT TGGCAACGAT GATGTCCACA TCATAGCACG CTCTGATGAT
    GAGATGCTTG ATGATTTTTT CCATGATCAG AGTACAGCCA CAAGTCAGGC TGGCACACTT
    TCCAGCATTC CAACTGCGCT GACCCGCTGG ACAGAAGAGT GTAAGGTCTT GGATGCTGAA
    AGCATGCATG ACTGTGTATC TGTGGTCAAA GTCCCAATCA TCAATCATTT GGAATCTTTG
    CGTGATGAGG AGCTTGAGGA GCGCAGGGAA AAAAGAAGGA AACAACTAGC AGAAGAGGAG
    GCCAAGATGG CAGAGAAATC CAAGGAGGAG AAGGAAGCAA AGGAGCAAAA TTCACAGGGT
    GCCAATTCAA AGCAGAATAC CCCATTTGAA CTGAGCCTTG CAGATCAAGA TGGAAGTGGT
    CGTCCTGAAA ATTTCCCAAC TGCACCATCT TCTGCAGAAG GAACTCCAAC AAGTCCTGAA
    ATTGTTCGTT TAGATCCCAC CCTAAGGGCT ACTGAGGCTA CTCTGGCAGA GCTGACTCAG
    GGTCTGCCTG CTGATACAGG CACGGAGGAT GTTGGGCAAC AACTCATGCC ATCTCACCAA
    GAGGACTCTG GACCAGGACG GCCAAGTGTA GAGGGGGATG CCACACAAAT GGAGCTATCG
    CCAGCACCCA CTATAACCTC CTTATCACCA GAGCGAGCAG AGGATTCAGA TGCTTTGACA
    GCTGTTAGTA GCCAACTAGA GGGCTCGCCT ATGGACACCA GCAGTCTTGC CTCTGGGCCC
    TTAGAGGATG GCGTAGGGGA GGCACCTGGA ATTGTTAATC CAGAGCAGCC TCTTGTGGTT
    GGCAGTAGCA TTGCTGTTGG ACAGCAACCA GTGGAACCAC CGACGACCTG TGAAACCCAA
    CCAGTAGTTT CTCAACACAG TGATAGCTCA CAAATTGCCC CTTCCTCTGA GTTGCCTTCC
    ACAAGAGAAA GTGCTGTGAC CTTGTTGGCT ACAGATTCTA GTGGTATCCT TGAAGAACCT
    TTGCCTTCTA CAAGCAGTGA AGAGGAAGAC CCATTAGCAG GCATAAATCT TCCAGAGGGT
    GTGGATCCTT CTTTCCTAGC GGCTCTTCCT GAAGACATCC GCAGAGAGGT GCTGCAGAAT
    CAGTTGGGCA TACGGCCGCC ATCGCGACCT CCACCCACTG CTGCCCCAGT TATTCCTCCT
    CCTGTGGTTG CTAATGCTGG TGTCACGGAG GTCAGCCCTG AGTTCTTGGC AGCGTTGCCA
    CCAGCCATTC AGGAAGAGGT CCTAGCACAG CAGCGAGCAG AACAGCAGAG GCGGGAACTT
    GCTCAGACCA CTACGTCTGA TACACCCATG GATCCTGTGA CTTTCATTCA GACGTTACCT
    TCTGATCTAC GTCGTAGTGT GTTAGAAGAC ATGGAGGACA GTGTTTTAGC TGTGATGCCA
    CCTGACATTG CTGCAGAGGC ACAGGCTCTA AGGCGAGAGC AAGAAGCTCG GCAGAGGCAA
    CTAATGCATG AACGACTGTT CGGGCACAGT AGCACATCTG CACTCTCTGC CATCCTTAGG
    AGTCCTGCTT TCACGAGTCG CTTGAGTGGA AACCGAGGTG TTCAGTACAC ACGGTTGGCT
    GTACAGAGAG GGAACACTTT CCAAATGGGA AGCAGCAGTA CACATACCAG ACCAACCGGT
    AGTAATGTTG ACAGCCTACT ACGATTACGA GGCCGACTAT TACTTGATCA TGAAGCCCTC
    TCCTGTCTGC TTGTCCTACT GTTTGTTGAT GAACCCAAGC TAAACACCAG TCGCTTGCAC
    CGTGTTCTAC GCAACCTCTG CTACCATGCA CAAACAAGGA ACTGGGTGAT TAGAAGCTTG
    TTGTCTATAC TGCAGCGTAG CAGTGAAAGT GAGCTTTGTA TAGAAAGCCC AAAGACTTCT
    ATTGGGGGCA CTGATAAACG GGGTGCAAAA ACAAGTCATG ACAACCGCCC TTTAGAATTT
    CTGCATAAGA TGGAATCGAA GAATTCCAAC CAGCTCTCCT GGCTTTCTGT ATCCATGGAC
    GCTGCTCTTG GCTGCCGTAC GAACATCTTC CAAATCCAGA GGTCCCTAGG ACGCAAGCAT
    ACAGAAAAGC ATTCAGCCTG TGGTTCTACT GTCCACATCC ATCCCCAAGC AGCCCCTGTA
    GTGTGTAGAC ATGTGCTTGA CACACTAATA CAGCTAGCTA AGGTGTTTCC CAGTCATTTC
    ACACAGCAGC GAACCAAAGA ATGTAGTGTG GAAAACAGTG ACAGCAGGGA GCGGAGTATA
    AAACTTACTT GTAGTCCGTG TCTTGCACCA TCTAGTAGTA TTGGAGCAAG CAACATCTCC
    ACAGATTTCT GGGACTTGCT AGTCAAGCTA GACAACATGA ATGTGAGCCG GAAGGGGAAA
    ACGTCTATAA AATCTGTGCC AGTGAGCTCT GGTTCTGAAG GCGAGAGCAA TCAGTGCAGC
    CTGGAGGCCT CCCCCTTAGG ACAGCTTATG AATATGCTGT CTCATTCTGT TATTCGCCGC
    AGTTCGCTGC TTACTGAAAA GCTGCTGAGA CTTCTCTCCC TTATCTCTAT TGCCCTTCCT
    GATAATAAGG CTTCTGAAAC AGCAGGCAAT CAGAGCAATT CTATTACCCC AGCACCTGCA
    CCTACGCCTG CAACTGCACC AGCCCCTGTG CCTGTACCTG CTGTAACAGT TCCAGTTGTT
    GTGAGCACAA ACATAGTGAC AACTGTTACA ACCAAAAGTG GAAAATCTCC TGCAAAATCA
    AGTGAAGTCA GCAATGCACC AGATAAGATG GCAGCTTCTG GCCTTACATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65588
    Clone ID Related Accession (Same CDS sequence) XM_031892168.1
    Accession Version XM_031892168.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGGTGCTG ATGGATATGG GTTTCACAAG GGAGCATGCT
    CTGGAAGCTC TGCTTAACAC GAGTACTATG GAGCAGGCCA CTGAATATCT CCTCACCCAC
    CCTCCACCTC TTATTGGCGG AGTTGTACGG GACTTAAGTA TGTCAGAGGA AGATCAGATG
    CTGAGAGCCA TTGCCATGTC GCTGGGACAA GATATCCCTA TGGAACAGAG GGCAGAACTG
    CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG GAGGAACGCA AGGCACGAGA AAAACAAGAG
    GAAGAGGAAG CTAAATGCTT GGAAAAGTTT CAAGATGCAG AACCCCTGGA ACAGGATGAG
    CTTCAAGGAT TCACAGACAC CATGCTCCCT GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA
    GACACAGTCT ATAGAGTGTG TGATCTTATA ATGACTGCCA TAAAGCGCAA TGGGGCAGAC
    TATCGAGACA TGATTCTAAA ACAAGTGGTT AACCAGGTAT GGGAAGCCGC TGATGTTCTT
    ATTAAAGCTG CACTGCCGCT GACCACAAGT GACACAAAAA CTGTGTCTGA GTGGATCAGC
    CAAATGGCTA CTCTTCCTCA AGCTTCTAAC TTGGCCACTC GCATCCTTTT GCTCACACTG
    CTGTTTGAGG AGCTAAAACT GCCATGTGCT AGAGTTGTTG AAACAAGTGG AATACTTAAC
    GTACTAATTA AGCTGCTTGA AGTGGTTCAG CCATGTTTGC AGGCAGCCAA GGAGCAAAAG
    GAAGTTCAGA CACCAAAGTG GATTACACCA GTCCTCCTCT TAATAGATTT TTATGAGAAG
    ACCTCAATTT CTTCTAAAAG AAGAATGCAA ATGAACAGGT ACCTTCAAGC TAATGGAAAC
    AACTGGCGAT GGTTTGATGA TCGGTCTGGC CGTTGGTGCA GCTACAGTGC TAGTAACAAC
    AGCACTATTG ATGCAGCCTG GAAAGCAGGA GAAACATGTG TGCGATTTAC AGCTGGCCGT
    CGAAGATATA CTGTGCAGTT TACCACAATG GTTCAGGTAA ATGAAGAGAC CGGCAATAGA
    CGACCTGTCA TGCTGACTCT CTTGCGTGTT CCGAGACTTT TAAAGAGTGG AAAAAATGGA
    AATGGACAGG AACTTGAAAA GACACTTGAG GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA
    GACGAAAAAG TGCCTGAGCC CATTCCAGTT CCTGAAACCT CTTCCACTGA GAAAGATGCA
    CCAACTGAAG AGCCCAAAGT TGGAGACATA GTAATTCAGG GTCTGGCTGA AGAAATGGTG
    ACCGTCCTTA TACGTTCCTG CGTCGGCATG CTTGGTGTTC CTGTTGATCC AGATACTCTT
    CATGCAACCT TGCGTCTTTG CCTGCGTCTC ACACGTGACC ACAAATATGC CATGATGTTT
    GCAGAGCTTA AGAGCACTCG AATGATCCTC AGCTTAACTC AAAGCTCTGG ATTCAATGGC
    TTTACTCCCC TTGTGACGCT CCTATTTAGG CACATCATAG AGGATCCTTG CACCCTCCAG
    CACACCATGG AAAAGGTTGT ACGGTCTGCA GCAACTAGTG GGGCAGGGAG TACCACATCT
    GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT GAAATCAACT ATATACTTAG AGTACTGGGT
    CCAGCAGCTT GTCGAAACCC TGACATTTTC ACTGAAGTGG CAAACAGTTG CATTCGCATT
    GCTCTCCCTG CACCTCGTGG ATCTGGAACT GCCTCTGATG ATGAGTTTGA GAACCTTCGG
    ATCAAAGGTC CTAATGCAGT TCAGTTGGTT AAAACCACCC CTATAAAGCC CTCCACCTTG
    CCAGTCATCC CTGATACAAT TAAGGAAGTA ATCTATGACA TGCTGAATGC ACTGGCAGCT
    TACCACGCCC CTGAGGAAGT ATCTGAAAAG TCTGAGCAAA AACCTGTTGG CATGAACCAG
    GAAGTCAGCC AGATCCTACA GGACATGGGT GATGAGGTTT TCCAGCAATA TCGGGACTTA
    ACCAGACAGG GAAGCGACTT TGATGGTCAG ACCAGTTTTG CCATCAACGC GCGAGTCTTT
    ACTGCTGACA GCACAGCTGC CGAAACACCT CAGTCTGGTA CACCTCAAGG TGAAACTGCC
    TTAACACCTG AAGAAAACCG AGAAGGGTTA AAGAAAGATA AGGAAGGAGA CCGTAGTTCA
    GAAGATGGAA AGCAGAAGGC AAAAGGTAGT AAGCCTTTGC TGCCTACCTC TTCCATTCTT
    CGACTACTTG CTGAACTTGT CAGGTCCTAT GTCGGAATAG CCTCGCTGAT CGCCACCTAC
    AATTACACTG CAGGACAATC TGAACTAATT AAAGAGGATT GCAGTGTATT GGCGTTTGTG
    CTGGATCACC TGCTACCCCA CACACATAAT GCAGAGGACA AGGACACACC AGCCCTAGCC
    CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA GGAAGTGGCA CTGATGCCCA GGTTGCACTA
    GTGAATGAAG TAAAGGCAGC TCTTGGCAGA GCCCTAGCAA TGGCTGAGAG CACGGAGAAG
    CATGCTAGGT TGCAGGCTGT GATGTGCATT ATCAGTACCA TTATGGAGTC ATGTCCATCC
    ACCTCCAGCT TCTACAGCAC AGCAACCACC AAAACCCAGC ACAATGGCAT GAACAACATC
    ATCAGACTCT TTTTGAAAAA GGGGCTGGTT AGTGATTTAG CCAGGGTCCC TCACAGCCTT
    GATTTATCCA GTCCAAATAT GGCAAACACC GTGAATGCAG CACTGAAGCC ACTCGAGACA
    CTGTCTCGCA TTGTCAATCA ACCCAGCAGT TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG
    AATAAATCTG AGGCAGATAC ACATGGAACA GCCCAGGATC CCAACAGTAA TCAGCCAGAT
    ACAGGTGAAA CAGGAACAGA AGTGCAGGAA GAGGACCCAG ATGCTTCTCA TGCTGAAGTA
    GCTGATGGAG ACATCATGGA TGGAGAGACA GAGACTGATG CTGTGGTTAT TGCTGGCCAG
    CCTGAAGTCC TTACTACACA AGAAATGCAG GTAGAGAATG AGCTTGAAGA TCTGATAGAT
    GAACTTCTTG AAAGGGATGG TGCTGCAGGT AACCGGGTCG GAGAAGATGA ATCACAGGAA
    GATGTGCTGA TGGATGAGCC TCCTACTAAC CTCAGCCAAG CCTCCACTCT TCAGGCTAAT
    CGAGAAGACT CTATGAACAT CTTAGACCCA GAGGATGAGG AAGAGCACAC CCAAGAAGAG
    GACAGCAGTG GCAGCAATGA AGATGAAGAT GACAGCCAGG AAGAGGAGGA GGAAGAGGAA
    GATGAGGACG ATCAGGATGA TGAGGAGGGT GAGGAAGGGG ATGAGGATGA TGATGATGAC
    TGTTCAGAGA TGGAACTGGA TGAGGATTAT CCAGACATGA ATGCCTCACC TCTTGTACGC
    TTTGAACGTT TTGACAGAGA AGATGATTTA ATCATAGAGT TTGATAACAT GTTCTCCAGT
    GCCACTGAGA TCCCTCCATC CCCTGGTAAC ATCCCTACTA CACACCCCCT AATGGTGCGA
    CACGCTGATC ACGGCTCCTT GACTCTTGGC AGTGGCACTT CCACTACTCG CCTGGCACAG
    GGCATCGGGC GCAGCCAGAG AACACTCCGA CAACTCACAG CCAACACCGG TCACACAATA
    CATGTTCATT ATCCTGGCAA CAGGCAGCCA AATCCACCTT TAATCCTCCA GAGGTTGCTG
    GGGCCCTCAG CCGCTGCTGA CATCTTGCAA CTAAGCAGCA GTCTTCCTCT GCAGAGTCGT
    GGCCGAGCAC GTCTACTTGT TGGCAACGAT GATGTCCACA TCATAGCACG CTCTGATGAT
    GAGATGCTTG ATGATTTTTT CCATGATCAG AGTACAGCCA CAAGTCAGGC TGGCACACTT
    TCCAGCATTC CAACTGCGCT GACCCGCTGG ACAGAAGAGT GTAAGGTCTT GGATGCTGAA
    AGCATGCATG ACTGTGTATC TGTGGTCAAA GTCCCAATCA TCAATCATTT GGAATCTTTG
    CGTGATGAGG AGCTTGAGGA GCGCAGGGAA AAAAGAAGGA AACAACTAGC AGAAGAGGAG
    GCCAAGATGG CAGAGAAATC CAAGGAGGAG AAGGAAGCAA AGGAGCAAAA TTCACAGGGT
    GCCAATTCAA AGCAGAATAC CCCATTTGAA CTGAGCCTTG CAGATCAAGA TGGAAGTGGT
    CGTCCTGAAA ATTTCCCAAC TGCACCATCT TCTGCAGAAG GAACTCCAAC AAGTCCTGAA
    ATTGTTCGTT TAGATCCCAC CCTAAGGGCT ACTGAGGCTA CTCTGGCAGA GCTGACTCAG
    GGTCTGCCTG CTGATACAGG CACGGAGGAT GTTGGGCAAC AACTCATGCC ATCTCACCAA
    GAGGACTCTG GACCAGGACG GCCAAGTGTA GAGGGGGATG CCACACAAAT GGAGCTATCG
    CCAGCACCCA CTATAACCTC CTTATCACCA GAGCGAGCAG AGGATTCAGA TGCTTTGACA
    GCTGTTAGTA GCCAACTAGA GGGCTCGCCT ATGGACACCA GCAGTCTTGC CTCTGGGCCC
    TTAGAGGATG GCGTAGGGGA GGCACCTGGA ATTGTTAATC CAGAGCAGCC TCTTGTGGTT
    GGCAGTAGCA TTGCTGTTGG ACAGCAACCA GTGGAACCAC CGACGACCTG TGAAACCCAA
    CCAGTAGTTT CTCAACACAG TGATAGCTCA CAAATTGCCC CTTCCTCTGA GTTGCCTTCC
    ACAAGAGAAA GTGCTGTGAC CTTGTTGGCT ACAGATTCTA GTGGTATCCT TGAAGAACCT
    TTGCCTTCTA CAAGCAGTGA AGAGGAAGAC CCATTAGCAG GCATAAATCT TCCAGAGGGT
    GTGGATCCTT CTTTCCTAGC GGCTCTTCCT GAAGACATCC GCAGAGAGGT GCTGCAGAAT
    CAGTTGGGCA TACGGCCGCC ATCGCGACCT CCACCCACTG CTGCCCCAGT TATTCCTCCT
    CCTGTGGTTG CTAATGCTGG TGTCACGGAG GTCAGCCCTG AGTTCTTGGC AGCGTTGCCA
    CCAGCCATTC AGGAAGAGGT CCTAGCACAG CAGCGAGCAG AACAGCAGAG GCGGGAACTT
    GCTCAGACCA CTACGTCTGA TACACCCATG GATCCTGTGA CTTTCATTCA GACGTTACCT
    TCTGATCTAC GTCGTAGTGT GTTAGAAGAC ATGGAGGACA GTGTTTTAGC TGTGATGCCA
    CCTGACATTG CTGCAGAGGC ACAGGCTCTA AGGCGAGAGC AAGAAGCTCG GCAGAGGCAA
    CTAATGCATG AACGACTGTT CGGGCACAGT AGCACATCTG CACTCTCTGC CATCCTTAGG
    AGTCCTGCTT TCACGAGTCG CTTGAGTGGA AACCGAGGTG TTCAGTACAC ACGGTTGGCT
    GTACAGAGAG GGAACACTTT CCAAATGGGA AGCAGCAGTA CACATACCAG ACCAACCGGT
    AGTAATGTTG ACAGCCTACT ACGATTACGA GGCCGACTAT TACTTGATCA TGAAGCCCTC
    TCCTGTCTGC TTGTCCTACT GTTTGTTGAT GAACCCAAGC TAAACACCAG TCGCTTGCAC
    CGTGTTCTAC GCAACCTCTG CTACCATGCA CAAACAAGGA ACTGGGTGAT TAGAAGCTTG
    TTGTCTATAC TGCAGCGTAG CAGTGAAAGT GAGCTTTGTA TAGAAAGCCC AAAGACTTCT
    ATTGGGGGCA CTGATAAACG GGGTGCAAAA ACAAGTCATG ACAACCGCCC TTTAGAATTT
    CTGCATAAGA TGGAATCGAA GAATTCCAAC CAGCTCTCCT GGCTTTCTGT ATCCATGGAC
    GCTGCTCTTG GCTGCCGTAC GAACATCTTC CAAATCCAGA GGTCCCTAGG ACGCAAGCAT
    ACAGAAAAGC ATTCAGCCTG TGGTTCTACT GTCCACATCC ATCCCCAAGC AGCCCCTGTA
    GTGTGTAGAC ATGTGCTTGA CACACTAATA CAGCTAGCTA AGGTGTTTCC CAGTCATTTC
    ACACAGCAGC GAACCAAAGA ATGTAGTGTG GAAAACAGTG ACAGCAGGGA GCGGAGTATA
    AAACTTACTT GTAGTCCGTG TCTTGCACCA TCTAGTAGTA TTGGAGCAAG CAACATCTCC
    ACAGATTTCT GGGACTTGCT AGTCAAGCTA GACAACATGA ATGTGAGCCG GAAGGGGAAA
    ACGTCTATAA AATCTGTGCC AGTGAGCTCT GGTTCTGAAG GCGAGAGCAA TCAGTGCAGC
    CTGGAGGCCT CCCCCTTAGG ACAGCTTATG AATATGCTGT CTCATTCTGT TATTCGCCGC
    AGTTCGCTGC TTACTGAAAA GCTGCTGAGA CTTCTCTCCC TTATCTCTAT TGCCCTTCCT
    GATAATAAGG CTTCTGAAAC AGCAGGCAAT CAGAGCAATT CTATTACCCC AGCACCTGCA
    CCTACGCCTG CAACTGCACC AGCCCCTGTG CCTGTACCTG CTGTAACAGT TCCAGTTGTT
    GTGAGCACAA ACATAGTGAC AACTGTTACA ACCAAAAGTG GAAAATCTCC TGCAAAATCA
    AGTGAAGTCA GCAATGCACC AGATAAGATG GCAGCTTCTG GCCTTACAGA GAAACAGCTG
    CAGTTGTCTG TTGAGGTTCT GACATCACAC TCGTGTTCAG AAGAAGGACT GGAGGATGCC
    GCCAATGTTC TACTTCAGCT TTCCAGAGGC GACCCCAGTA CACGGGACAC TGTACTGAAA
    CTTCTGCTTA GCGGAGCACA TCACTTGGGG TTTACTCTCT GCAAACAGAT AGGTACACTA
    CTAGCAGAGC TCCGGGAATA TAACCTAGAG CAGCAGCGTC GTGCACAGTG TGAAGTTCTT
    TCTCCAGATG GTATGCCTGA GGAGCAACCC CACAGCACAA AGCCAAAAGG CAAAATGCAG
    AGCAGTGTTA GGTCTCTCCC CACCCCACCT ATGCGATCCA GGTTCGACAT TGCAGAGAAT
    GTGGTGATTG TGGCTTCCCA AAAGCGTCCA CTGGGAGGAA GAGAGCTGCA GCTCCCCTCA
    ATGTCTATGC TGACGTCAAA GACTTCCACA CAAAAATTCT TTCTTCGAGT GCTGCAGGTT
    ATCATCCAGC TTCGCGATGA CACTCGCAGA GCCAACAAGA AGGCCAAGCA GACCAGCAGA
    TTAGGCACTG CCAGTCTGGC CTCGGCTAGC AGCATCCAAG CAGCTGTGCA GCAGCTGGAG
    GCTGAGGCTG ATGCCATTAT ACAAATGGTA CGTGAGGGTC AAAGGGCGCG GAGACAGCTG
    CAAACTGCAA CGGCGGAGGG TTCTGCTCGC AGGGAGGAGC AGCCTATGGA TGTTGACCAG
    CCTTCGCCTG CTACACAGGA GCCTCAGTCA GTTGCTTCTG AAGGTTCTCA GGCTGCTGAA
    AAAAAAGAAG AGGAGAGGTT ACCAGAGTTA CCGCTGTTAA GTGAGCAGCT AAGTTTAGAT
    GAGCTGTGGG ACATGTTGGG AGAGTGCCTG AAGGAACTTG AGGAATCTCA TGACCAGCAT
    GCCGTATTAG TGTTACAGCC AGCCGTAGAA GCCTTTTTCC TTGTTCATGC AACAGAGCGG
    GAGAGCAAGC CTCCTGTGCG GGACACTCGG GAAAGCCAGT TATCTCACAT TAAGGATGAA
    CCACCTCCCC TGTCTCCTGC ACCCCTCACT CCAGCCACAC CTTCTTCCTT GGATCCTTTC
    TTCTCTCGTG AACCTTCATC TATGCACATT TCCTCTAGTT TGCCTCCTGA CACTCAGAAG
    TTCCTAAGAT TTGCTGAGAC TCATCGGACT GTATTAAATC AGATCCTTCG CCAGTCCACC
    ACACACCTAG CTGATGGGCC CTTTGCTGTG CTTGTAGACT ATATCAGAGT CCTTGACTTT
    GATGTTAAGC GCAAATATTT CCGACAGGAG TTGGAGCGAC TGGATGAAGG GCTAAGAAAA
    GAGGATATGG CAGTGCATGT GCGGCGAGAT CATGTGTTTG AGGATTCCTA CCGGGAGCTG
    CACCGCAAAT CCCCTGAAGA AATGAAGAAT AGATTGTACA TAGTATTTGA AGGAGAAGAA
    GGGCAGGATG CAGGTGGGCT GCTTCGGGAG TGGTACATGA TTATCTCCAG GGAGATGTTC
    AATCCCATGT ATGCTTTGTT CCGCACTTCC CCAGGTGACC GCGTCACCTA CACTATTAAT
    CCTTCCTCCC ACTGTAACCC TAACCACCTC AGCTACTTCA AGTTTGTGGG CCGGATAGTT
    GCCAAAGCGG TTTATGACAA CCGGCTACTG GAGTGTTACT TCACTAGGTC CTTCTACAAG
    CATATATTGG GCAAGTCTGT CAGATACACA GACATGGAGA GTGAAGATTA TCACTTCTAT
    CAGGGTCTGG TGTACTTGCT TGAAAATGAT GTCTCCACAC TGGGATATGA CTTGACTTTC
    AGCACTGAGG TTCAAGAATT TGGTGTCTGT GAAGTCCGAG ACCTAAAACC AAACGGTGCA
    AACATTCTGG TAACAGAAGA AAACAAAAAG GAGTATGTTC ATCTTGTGTG CCAGATGAAG
    ATGACAGGTG CAATACGTAA GCAATTGGCT GCCTTCCTTG AGGGATTTTA TGAGATAATC
    CCCAAACGTC TGATCTCCAT CTTCACTGAG CAGGAATTGG AACTGCTTAT CTCTGGCCTT
    CCCACCATAG ATATTGATGA CCTAAAATCA AACACTGAGT ATCACAAATA CCAGTCTAAC
    TCCATCCAGA TTCAGTGGTT CTGGAGAGCG TTGCGCTCAT TCGATCAAGC TGACCGGGCA
    AAGTTCCTTC AGTTTGTTAC TGGAACTTCA AAGGTCCCAT TACAGGGTTT TGCTGCGCTG
    GAAGGAATGA ACGGCATCCA GAAATTCCAA ATCCACAGAG ATGATAGATC TACAGACAGG
    CTGCCTTCTG CGCACACGTG CTTCAACCAG TTGGACCTTC CAGCCTATGA GAGTTACGAG
    AAGCTACGTC ACATGCTGCT GTTAGCCATC CAGGAGTGCT CTGAGGGCTT TGGCCTTGCC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748028.1
    CDS671..13813
    Translation

    Target ORF information:

    RefSeq Version XM_031892168.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892168.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGGTGCTG ATGGATATGG GTTTCACAAG GGAGCATGCT
    CTGGAAGCTC TGCTTAACAC GAGTACTATG GAGCAGGCCA CTGAATATCT CCTCACCCAC
    CCTCCACCTC TTATTGGCGG AGTTGTACGG GACTTAAGTA TGTCAGAGGA AGATCAGATG
    CTGAGAGCCA TTGCCATGTC GCTGGGACAA GATATCCCTA TGGAACAGAG GGCAGAACTG
    CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG GAGGAACGCA AGGCACGAGA AAAACAAGAG
    GAAGAGGAAG CTAAATGCTT GGAAAAGTTT CAAGATGCAG AACCCCTGGA ACAGGATGAG
    CTTCAAGGAT TCACAGACAC CATGCTCCCT GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA
    GACACAGTCT ATAGAGTGTG TGATCTTATA ATGACTGCCA TAAAGCGCAA TGGGGCAGAC
    TATCGAGACA TGATTCTAAA ACAAGTGGTT AACCAGGTAT GGGAAGCCGC TGATGTTCTT
    ATTAAAGCTG CACTGCCGCT GACCACAAGT GACACAAAAA CTGTGTCTGA GTGGATCAGC
    CAAATGGCTA CTCTTCCTCA AGCTTCTAAC TTGGCCACTC GCATCCTTTT GCTCACACTG
    CTGTTTGAGG AGCTAAAACT GCCATGTGCT AGAGTTGTTG AAACAAGTGG AATACTTAAC
    GTACTAATTA AGCTGCTTGA AGTGGTTCAG CCATGTTTGC AGGCAGCCAA GGAGCAAAAG
    GAAGTTCAGA CACCAAAGTG GATTACACCA GTCCTCCTCT TAATAGATTT TTATGAGAAG
    ACCTCAATTT CTTCTAAAAG AAGAATGCAA ATGAACAGGT ACCTTCAAGC TAATGGAAAC
    AACTGGCGAT GGTTTGATGA TCGGTCTGGC CGTTGGTGCA GCTACAGTGC TAGTAACAAC
    AGCACTATTG ATGCAGCCTG GAAAGCAGGA GAAACATGTG TGCGATTTAC AGCTGGCCGT
    CGAAGATATA CTGTGCAGTT TACCACAATG GTTCAGGTAA ATGAAGAGAC CGGCAATAGA
    CGACCTGTCA TGCTGACTCT CTTGCGTGTT CCGAGACTTT TAAAGAGTGG AAAAAATGGA
    AATGGACAGG AACTTGAAAA GACACTTGAG GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA
    GACGAAAAAG TGCCTGAGCC CATTCCAGTT CCTGAAACCT CTTCCACTGA GAAAGATGCA
    CCAACTGAAG AGCCCAAAGT TGGAGACATA GTAATTCAGG GTCTGGCTGA AGAAATGGTG
    ACCGTCCTTA TACGTTCCTG CGTCGGCATG CTTGGTGTTC CTGTTGATCC AGATACTCTT
    CATGCAACCT TGCGTCTTTG CCTGCGTCTC ACACGTGACC ACAAATATGC CATGATGTTT
    GCAGAGCTTA AGAGCACTCG AATGATCCTC AGCTTAACTC AAAGCTCTGG ATTCAATGGC
    TTTACTCCCC TTGTGACGCT CCTATTTAGG CACATCATAG AGGATCCTTG CACCCTCCAG
    CACACCATGG AAAAGGTTGT ACGGTCTGCA GCAACTAGTG GGGCAGGGAG TACCACATCT
    GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT GAAATCAACT ATATACTTAG AGTACTGGGT
    CCAGCAGCTT GTCGAAACCC TGACATTTTC ACTGAAGTGG CAAACAGTTG CATTCGCATT
    GCTCTCCCTG CACCTCGTGG ATCTGGAACT GCCTCTGATG ATGAGTTTGA GAACCTTCGG
    ATCAAAGGTC CTAATGCAGT TCAGTTGGTT AAAACCACCC CTATAAAGCC CTCCACCTTG
    CCAGTCATCC CTGATACAAT TAAGGAAGTA ATCTATGACA TGCTGAATGC ACTGGCAGCT
    TACCACGCCC CTGAGGAAGT ATCTGAAAAG TCTGAGCAAA AACCTGTTGG CATGAACCAG
    GAAGTCAGCC AGATCCTACA GGACATGGGT GATGAGGTTT TCCAGCAATA TCGGGACTTA
    ACCAGACAGG GAAGCGACTT TGATGGTCAG ACCAGTTTTG CCATCAACGC GCGAGTCTTT
    ACTGCTGACA GCACAGCTGC CGAAACACCT CAGTCTGGTA CACCTCAAGG TGAAACTGCC
    TTAACACCTG AAGAAAACCG AGAAGGGTTA AAGAAAGATA AGGAAGGAGA CCGTAGTTCA
    GAAGATGGAA AGCAGAAGGC AAAAGGTAGT AAGCCTTTGC TGCCTACCTC TTCCATTCTT
    CGACTACTTG CTGAACTTGT CAGGTCCTAT GTCGGAATAG CCTCGCTGAT CGCCACCTAC
    AATTACACTG CAGGACAATC TGAACTAATT AAAGAGGATT GCAGTGTATT GGCGTTTGTG
    CTGGATCACC TGCTACCCCA CACACATAAT GCAGAGGACA AGGACACACC AGCCCTAGCC
    CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA GGAAGTGGCA CTGATGCCCA GGTTGCACTA
    GTGAATGAAG TAAAGGCAGC TCTTGGCAGA GCCCTAGCAA TGGCTGAGAG CACGGAGAAG
    CATGCTAGGT TGCAGGCTGT GATGTGCATT ATCAGTACCA TTATGGAGTC ATGTCCATCC
    ACCTCCAGCT TCTACAGCAC AGCAACCACC AAAACCCAGC ACAATGGCAT GAACAACATC
    ATCAGACTCT TTTTGAAAAA GGGGCTGGTT AGTGATTTAG CCAGGGTCCC TCACAGCCTT
    GATTTATCCA GTCCAAATAT GGCAAACACC GTGAATGCAG CACTGAAGCC ACTCGAGACA
    CTGTCTCGCA TTGTCAATCA ACCCAGCAGT TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG
    AATAAATCTG AGGCAGATAC ACATGGAACA GCCCAGGATC CCAACAGTAA TCAGCCAGAT
    ACAGGTGAAA CAGGAACAGA AGTGCAGGAA GAGGACCCAG ATGCTTCTCA TGCTGAAGTA
    GCTGATGGAG ACATCATGGA TGGAGAGACA GAGACTGATG CTGTGGTTAT TGCTGGCCAG
    CCTGAAGTCC TTACTACACA AGAAATGCAG GTAGAGAATG AGCTTGAAGA TCTGATAGAT
    GAACTTCTTG AAAGGGATGG TGCTGCAGGT AACCGGGTCG GAGAAGATGA ATCACAGGAA
    GATGTGCTGA TGGATGAGCC TCCTACTAAC CTCAGCCAAG CCTCCACTCT TCAGGCTAAT
    CGAGAAGACT CTATGAACAT CTTAGACCCA GAGGATGAGG AAGAGCACAC CCAAGAAGAG
    GACAGCAGTG GCAGCAATGA AGATGAAGAT GACAGCCAGG AAGAGGAGGA GGAAGAGGAA
    GATGAGGACG ATCAGGATGA TGAGGAGGGT GAGGAAGGGG ATGAGGATGA TGATGATGAC
    TGTTCAGAGA TGGAACTGGA TGAGGATTAT CCAGACATGA ATGCCTCACC TCTTGTACGC
    TTTGAACGTT TTGACAGAGA AGATGATTTA ATCATAGAGT TTGATAACAT GTTCTCCAGT
    GCCACTGAGA TCCCTCCATC CCCTGGTAAC ATCCCTACTA CACACCCCCT AATGGTGCGA
    CACGCTGATC ACGGCTCCTT GACTCTTGGC AGTGGCACTT CCACTACTCG CCTGGCACAG
    GGCATCGGGC GCAGCCAGAG AACACTCCGA CAACTCACAG CCAACACCGG TCACACAATA
    CATGTTCATT ATCCTGGCAA CAGGCAGCCA AATCCACCTT TAATCCTCCA GAGGTTGCTG
    GGGCCCTCAG CCGCTGCTGA CATCTTGCAA CTAAGCAGCA GTCTTCCTCT GCAGAGTCGT
    GGCCGAGCAC GTCTACTTGT TGGCAACGAT GATGTCCACA TCATAGCACG CTCTGATGAT
    GAGATGCTTG ATGATTTTTT CCATGATCAG AGTACAGCCA CAAGTCAGGC TGGCACACTT
    TCCAGCATTC CAACTGCGCT GACCCGCTGG ACAGAAGAGT GTAAGGTCTT GGATGCTGAA
    AGCATGCATG ACTGTGTATC TGTGGTCAAA GTCCCAATCA TCAATCATTT GGAATCTTTG
    CGTGATGAGG AGCTTGAGGA GCGCAGGGAA AAAAGAAGGA AACAACTAGC AGAAGAGGAG
    GCCAAGATGG CAGAGAAATC CAAGGAGGAG AAGGAAGCAA AGGAGCAAAA TTCACAGGGT
    GCCAATTCAA AGCAGAATAC CCCATTTGAA CTGAGCCTTG CAGATCAAGA TGGAAGTGGT
    CGTCCTGAAA ATTTCCCAAC TGCACCATCT TCTGCAGAAG GAACTCCAAC AAGTCCTGAA
    ATTGTTCGTT TAGATCCCAC CCTAAGGGCT ACTGAGGCTA CTCTGGCAGA GCTGACTCAG
    GGTCTGCCTG CTGATACAGG CACGGAGGAT GTTGGGCAAC AACTCATGCC ATCTCACCAA
    GAGGACTCTG GACCAGGACG GCCAAGTGTA GAGGGGGATG CCACACAAAT GGAGCTATCG
    CCAGCACCCA CTATAACCTC CTTATCACCA GAGCGAGCAG AGGATTCAGA TGCTTTGACA
    GCTGTTAGTA GCCAACTAGA GGGCTCGCCT ATGGACACCA GCAGTCTTGC CTCTGGGCCC
    TTAGAGGATG GCGTAGGGGA GGCACCTGGA ATTGTTAATC CAGAGCAGCC TCTTGTGGTT
    GGCAGTAGCA TTGCTGTTGG ACAGCAACCA GTGGAACCAC CGACGACCTG TGAAACCCAA
    CCAGTAGTTT CTCAACACAG TGATAGCTCA CAAATTGCCC CTTCCTCTGA GTTGCCTTCC
    ACAAGAGAAA GTGCTGTGAC CTTGTTGGCT ACAGATTCTA GTGGTATCCT TGAAGAACCT
    TTGCCTTCTA CAAGCAGTGA AGAGGAAGAC CCATTAGCAG GCATAAATCT TCCAGAGGGT
    GTGGATCCTT CTTTCCTAGC GGCTCTTCCT GAAGACATCC GCAGAGAGGT GCTGCAGAAT
    CAGTTGGGCA TACGGCCGCC ATCGCGACCT CCACCCACTG CTGCCCCAGT TATTCCTCCT
    CCTGTGGTTG CTAATGCTGG TGTCACGGAG GTCAGCCCTG AGTTCTTGGC AGCGTTGCCA
    CCAGCCATTC AGGAAGAGGT CCTAGCACAG CAGCGAGCAG AACAGCAGAG GCGGGAACTT
    GCTCAGACCA CTACGTCTGA TACACCCATG GATCCTGTGA CTTTCATTCA GACGTTACCT
    TCTGATCTAC GTCGTAGTGT GTTAGAAGAC ATGGAGGACA GTGTTTTAGC TGTGATGCCA
    CCTGACATTG CTGCAGAGGC ACAGGCTCTA AGGCGAGAGC AAGAAGCTCG GCAGAGGCAA
    CTAATGCATG AACGACTGTT CGGGCACAGT AGCACATCTG CACTCTCTGC CATCCTTAGG
    AGTCCTGCTT TCACGAGTCG CTTGAGTGGA AACCGAGGTG TTCAGTACAC ACGGTTGGCT
    GTACAGAGAG GGAACACTTT CCAAATGGGA AGCAGCAGTA CACATACCAG ACCAACCGGT
    AGTAATGTTG ACAGCCTACT ACGATTACGA GGCCGACTAT TACTTGATCA TGAAGCCCTC
    TCCTGTCTGC TTGTCCTACT GTTTGTTGAT GAACCCAAGC TAAACACCAG TCGCTTGCAC
    CGTGTTCTAC GCAACCTCTG CTACCATGCA CAAACAAGGA ACTGGGTGAT TAGAAGCTTG
    TTGTCTATAC TGCAGCGTAG CAGTGAAAGT GAGCTTTGTA TAGAAAGCCC AAAGACTTCT
    ATTGGGGGCA CTGATAAACG GGGTGCAAAA ACAAGTCATG ACAACCGCCC TTTAGAATTT
    CTGCATAAGA TGGAATCGAA GAATTCCAAC CAGCTCTCCT GGCTTTCTGT ATCCATGGAC
    GCTGCTCTTG GCTGCCGTAC GAACATCTTC CAAATCCAGA GGTCCCTAGG ACGCAAGCAT
    ACAGAAAAGC ATTCAGCCTG TGGTTCTACT GTCCACATCC ATCCCCAAGC AGCCCCTGTA
    GTGTGTAGAC ATGTGCTTGA CACACTAATA CAGCTAGCTA AGGTGTTTCC CAGTCATTTC
    ACACAGCAGC GAACCAAAGA ATGTAGTGTG GAAAACAGTG ACAGCAGGGA GCGGAGTATA
    AAACTTACTT GTAGTCCGTG TCTTGCACCA TCTAGTAGTA TTGGAGCAAG CAACATCTCC
    ACAGATTTCT GGGACTTGCT AGTCAAGCTA GACAACATGA ATGTGAGCCG GAAGGGGAAA
    ACGTCTATAA AATCTGTGCC AGTGAGCTCT GGTTCTGAAG GCGAGAGCAA TCAGTGCAGC
    CTGGAGGCCT CCCCCTTAGG ACAGCTTATG AATATGCTGT CTCATTCTGT TATTCGCCGC
    AGTTCGCTGC TTACTGAAAA GCTGCTGAGA CTTCTCTCCC TTATCTCTAT TGCCCTTCCT
    GATAATAAGG CTTCTGAAAC AGCAGGCAAT CAGAGCAATT CTATTACCCC AGCACCTGCA
    CCTACGCCTG CAACTGCACC AGCCCCTGTG CCTGTACCTG CTGTAACAGT TCCAGTTGTT
    GTGAGCACAA ACATAGTGAC AACTGTTACA ACCAAAAGTG GAAAATCTCC TGCAAAATCA
    AGTGAAGTCA GCAATGCACC AGATAAGATG GCAGCTTCTG GCCTTACAGA GAAACAGCTG
    CAGTTGTCTG TTGAGGTTCT GACATCACAC TCGTGTTCAG AAGAAGGACT GGAGGATGCC
    GCCAATGTTC TACTTCAGCT TTCCAGAGGC GACCCCAGTA CACGGGACAC TGTACTGAAA
    CTTCTGCTTA GCGGAGCACA TCACTTGGGG TTTACTCTCT GCAAACAGAT AGGTACACTA
    CTAGCAGAGC TCCGGGAATA TAACCTAGAG CAGCAGCGTC GTGCACAGTG TGAAGTTCTT
    TCTCCAGATG GTATGCCTGA GGAGCAACCC CACAGCACAA AGCCAAAAGG CAAAATGCAG
    AGCAGTGTTA GGTCTCTCCC CACCCCACCT ATGCGATCCA GGTTCGACAT TGCAGAGAAT
    GTGGTGATTG TGGCTTCCCA AAAGCGTCCA CTGGGAGGAA GAGAGCTGCA GCTCCCCTCA
    ATGTCTATGC TGACGTCAAA GACTTCCACA CAAAAATTCT TTCTTCGAGT GCTGCAGGTT
    ATCATCCAGC TTCGCGATGA CACTCGCAGA GCCAACAAGA AGGCCAAGCA GACCAGCAGA
    TTAGGCACTG CCAGTCTGGC CTCGGCTAGC AGCATCCAAG CAGCTGTGCA GCAGCTGGAG
    GCTGAGGCTG ATGCCATTAT ACAAATGGTA CGTGAGGGTC AAAGGGCGCG GAGACAGCTG
    CAAACTGCAA CGGCGGAGGG TTCTGCTCGC AGGGAGGAGC AGCCTATGGA TGTTGACCAG
    CCTTCGCCTG CTACACAGGA GCCTCAGTCA GTTGCTTCTG AAGGTTCTCA GGCTGCTGAA
    AAAAAAGAAG AGGAGAGGTT ACCAGAGTTA CCGCTGTTAA GTGAGCAGCT AAGTTTAGAT
    GAGCTGTGGG ACATGTTGGG AGAGTGCCTG AAGGAACTTG AGGAATCTCA TGACCAGCAT
    GCCGTATTAG TGTTACAGCC AGCCGTAGAA GCCTTTTTCC TTGTTCATGC AACAGAGCGG
    GAGAGCAAGC CTCCTGTGCG GGACACTCGG GAAAGCCAGT TATCTCACAT TAAGGATGAA
    CCACCTCCCC TGTCTCCTGC ACCCCTCACT CCAGCCACAC CTTCTTCCTT GGATCCTTTC
    TTCTCTCGTG AACCTTCATC TATGCACATT TCCTCTAGTT TGCCTCCTGA CACTCAGAAG
    TTCCTAAGAT TTGCTGAGAC TCATCGGACT GTATTAAATC AGATCCTTCG CCAGTCCACC
    ACACACCTAG CTGATGGGCC CTTTGCTGTG CTTGTAGACT ATATCAGAGT CCTTGACTTT
    GATGTTAAGC GCAAATATTT CCGACAGGAG TTGGAGCGAC TGGATGAAGG GCTAAGAAAA
    GAGGATATGG CAGTGCATGT GCGGCGAGAT CATGTGTTTG AGGATTCCTA CCGGGAGCTG
    CACCGCAAAT CCCCTGAAGA AATGAAGAAT AGATTGTACA TAGTATTTGA AGGAGAAGAA
    GGGCAGGATG CAGGTGGGCT GCTTCGGGAG TGGTACATGA TTATCTCCAG GGAGATGTTC
    AATCCCATGT ATGCTTTGTT CCGCACTTCC CCAGGTGACC GCGTCACCTA CACTATTAAT
    CCTTCCTCCC ACTGTAACCC TAACCACCTC AGCTACTTCA AGTTTGTGGG CCGGATAGTT
    GCCAAAGCGG TTTATGACAA CCGGCTACTG GAGTGTTACT TCACTAGGTC CTTCTACAAG
    CATATATTGG GCAAGTCTGT CAGATACACA GACATGGAGA GTGAAGATTA TCACTTCTAT
    CAGGGTCTGG TGTACTTGCT TGAAAATGAT GTCTCCACAC TGGGATATGA CTTGACTTTC
    AGCACTGAGG TTCAAGAATT TGGTGTCTGT GAAGTCCGAG ACCTAAAACC AAACGGTGCA
    AACATTCTGG TAACAGAAGA AAACAAAAAG GAGTATGTTC ATCTTGTGTG CCAGATGAAG
    ATGACAGGTG CAATACGTAA GCAATTGGCT GCCTTCCTTG AGGGATTTTA TGAGATAATC
    CCCAAACGTC TGATCTCCAT CTTCACTGAG CAGGAATTGG AACTGCTTAT CTCTGGCCTT
    CCCACCATAG ATATTGATGA CCTAAAATCA AACACTGAGT ATCACAAATA CCAGTCTAAC
    TCCATCCAGA TTCAGTGGTT CTGGAGAGCG TTGCGCTCAT TCGATCAAGC TGACCGGGCA
    AAGTTCCTTC AGTTTGTTAC TGGAACTTCA AAGGTCCCAT TACAGGGTTT TGCTGCGCTG
    GAAGGAATGA ACGGCATCCA GAAATTCCAA ATCCACAGAG ATGATAGATC TACAGACAGG
    CTGCCTTCTG CGCACACGTG CTTCAACCAG TTGGACCTTC CAGCCTATGA GAGTTACGAG
    AAGCTACGTC ACATGCTGCT GTTAGCCATC CAGGAGTGCT CTGAGGGCTT TGGCCTTGCC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65589
    Clone ID Related Accession (Same CDS sequence) XM_031892169.1
    Accession Version XM_031892169.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGCTGATG GATATGGGTT TCACAAGGGA GCATGCTCTG
    GAAGCTCTGC TTAACACGAG TACTATGGAG CAGGCCACTG AATATCTCCT CACCCACCCT
    CCACCTCTTA TTGGCGGAGT TGTACGGGAC TTAAGTATGT CAGAGGAAGA TCAGATGCTG
    AGAGCCATTG CCATGTCGCT GGGACAAGAT ATCCCTATGG AACAGAGGGC AGAACTGCCC
    GAGGAAACTG CTCGGCGGAA AGAAGAGGAG GAACGCAAGG CACGAGAAAA ACAAGAGGAA
    GAGGAAGCTA AATGCTTGGA AAAGTTTCAA GATGCAGAAC CCCTGGAACA GGATGAGCTT
    CAAGGATTCA CAGACACCAT GCTCCCTGGC TGCTTCCACT TGCTTGATGA GCTGCCAGAC
    ACAGTCTATA GAGTGTGTGA TCTTATAATG ACTGCCATAA AGCGCAATGG GGCAGACTAT
    CGAGACATGA TTCTAAAACA AGTGGTTAAC CAGGTATGGG AAGCCGCTGA TGTTCTTATT
    AAAGCTGCAC TGCCGCTGAC CACAAGTGAC ACAAAAACTG TGTCTGAGTG GATCAGCCAA
    ATGGCTACTC TTCCTCAAGC TTCTAACTTG GCCACTCGCA TCCTTTTGCT CACACTGCTG
    TTTGAGGAGC TAAAACTGCC ATGTGCTAGA GTTGTTGAAA CAAGTGGAAT ACTTAACGTA
    CTAATTAAGC TGCTTGAAGT GGTTCAGCCA TGTTTGCAGG CAGCCAAGGA GCAAAAGGAA
    GTTCAGACAC CAAAGTGGAT TACACCAGTC CTCCTCTTAA TAGATTTTTA TGAGAAGACC
    TCAATTTCTT CTAAAAGAAG AATGCAAATG AACAGGTACC TTCAAGCTAA TGGAAACAAC
    TGGCGATGGT TTGATGATCG GTCTGGCCGT TGGTGCAGCT ACAGTGCTAG TAACAACAGC
    ACTATTGATG CAGCCTGGAA AGCAGGAGAA ACATGTGTGC GATTTACAGC TGGCCGTCGA
    AGATATACTG TGCAGTTTAC CACAATGGTT CAGGTAAATG AAGAGACCGG CAATAGACGA
    CCTGTCATGC TGACTCTCTT GCGTGTTCCG AGACTTTTAA AGAGTGGAAA AAATGGAAAT
    GGACAGGAAC TTGAAAAGAC ACTTGAGGAG GCAAAGGAGG TTGAAGCAAA GCAAAAAGAC
    GAAAAAGTGC CTGAGCCCAT TCCAGTTCCT GAAACCTCTT CCACTGAGAA AGATGCACCA
    ACTGAAGAGC CCAAAGTTGG AGACATAGTA ATTCAGGGTC TGGCTGAAGA AATGGTGACC
    GTCCTTATAC GTTCCTGCGT CGGCATGCTT GGTGTTCCTG TTGATCCAGA TACTCTTCAT
    GCAACCTTGC GTCTTTGCCT GCGTCTCACA CGTGACCACA AATATGCCAT GATGTTTGCA
    GAGCTTAAGA GCACTCGAAT GATCCTCAGC TTAACTCAAA GCTCTGGATT CAATGGCTTT
    ACTCCCCTTG TGACGCTCCT ATTTAGGCAC ATCATAGAGG ATCCTTGCAC CCTCCAGCAC
    ACCATGGAAA AGGTTGTACG GTCTGCAGCA ACTAGTGGGG CAGGGAGTAC CACATCTGGG
    GTTGTGTCTG GAAGCCTGGG TTCGCGTGAA ATCAACTATA TACTTAGAGT ACTGGGTCCA
    GCAGCTTGTC GAAACCCTGA CATTTTCACT GAAGTGGCAA ACAGTTGCAT TCGCATTGCT
    CTCCCTGCAC CTCGTGGATC TGGAACTGCC TCTGATGATG AGTTTGAGAA CCTTCGGATC
    AAAGGTCCTA ATGCAGTTCA GTTGGTTAAA ACCACCCCTA TAAAGCCCTC CACCTTGCCA
    GTCATCCCTG ATACAATTAA GGAAGTAATC TATGACATGC TGAATGCACT GGCAGCTTAC
    CACGCCCCTG AGGAAGTATC TGAAAAGTCT GAGCAAAAAC CTGTTGGCAT GAACCAGGAA
    GTCAGCCAGA TCCTACAGGA CATGGGTGAT GAGGTTTTCC AGCAATATCG GGACTTAACC
    AGACAGGGAA GCGACTTTGA TGGTCAGACC AGTTTTGCCA TCAACGCGCG AGTCTTTACT
    GCTGACAGCA CAGCTGCCGA AACACCTCAG TCTGGTACAC CTCAAGGTGA AACTGCCTTA
    ACACCTGAAG AAAACCGAGA AGGGTTAAAG AAAGATAAGG AAGGAGACCG TAGTTCAGAA
    GATGGAAAGC AGAAGGCAAA AGGTAGTAAG CCTTTGCTGC CTACCTCTTC CATTCTTCGA
    CTACTTGCTG AACTTGTCAG GTCCTATGTC GGAATAGCCT CGCTGATCGC CACCTACAAT
    TACACTGCAG GACAATCTGA ACTAATTAAA GAGGATTGCA GTGTATTGGC GTTTGTGCTG
    GATCACCTGC TACCCCACAC ACATAATGCA GAGGACAAGG ACACACCAGC CCTAGCCCGC
    CTCTTCTTAG CCAGCCTTGC TGCAGCAGGA AGTGGCACTG ATGCCCAGGT TGCACTAGTG
    AATGAAGTAA AGGCAGCTCT TGGCAGAGCC CTAGCAATGG CTGAGAGCAC GGAGAAGCAT
    GCTAGGTTGC AGGCTGTGAT GTGCATTATC AGTACCATTA TGGAGTCATG TCCATCCACC
    TCCAGCTTCT ACAGCACAGC AACCACCAAA ACCCAGCACA ATGGCATGAA CAACATCATC
    AGACTCTTTT TGAAAAAGGG GCTGGTTAGT GATTTAGCCA GGGTCCCTCA CAGCCTTGAT
    TTATCCAGTC CAAATATGGC AAACACCGTG AATGCAGCAC TGAAGCCACT CGAGACACTG
    TCTCGCATTG TCAATCAACC CAGCAGTTTG TTTGGAAGCA AAGGGACTTC AGGAAAGAAT
    AAATCTGAGG CAGATACACA TGGAACAGCC CAGGATCCCA ACAGTAATCA GCCAGATACA
    GGTGAAACAG GAACAGAAGT GCAGGAAGAG GACCCAGATG CTTCTCATGC TGAAGTAGCT
    GATGGAGACA TCATGGATGG AGAGACAGAG ACTGATGCTG TGGTTATTGC TGGCCAGCCT
    GAAGTCCTTA CTACACAAGA AATGCAGGTA GAGAATGAGC TTGAAGATCT GATAGATGAA
    CTTCTTGAAA GGGATGGTGC TGCAGGTAAC CGGGTCGGAG AAGATGAATC ACAGGAAGAT
    GTGCTGATGG ATGAGCCTCC TACTAACCTC AGCCAAGCCT CCACTCTTCA GGCTAATCGA
    GAAGACTCTA TGAACATCTT AGACCCAGAG GATGAGGAAG AGCACACCCA AGAAGAGGAC
    AGCAGTGGCA GCAATGAAGA TGAAGATGAC AGCCAGGAAG AGGAGGAGGA AGAGGAAGAT
    GAGGACGATC AGGATGATGA GGAGGGTGAG GAAGGGGATG AGGATGATGA TGATGACTGT
    TCAGAGATGG AACTGGATGA GGATTATCCA GACATGAATG CCTCACCTCT TGTACGCTTT
    GAACGTTTTG ACAGAGAAGA TGATTTAATC ATAGAGTTTG ATAACATGTT CTCCAGTGCC
    ACTGAGATCC CTCCATCCCC TGGTAACATC CCTACTACAC ACCCCCTAAT GGTGCGACAC
    GCTGATCACG GCTCCTTGAC TCTTGGCAGT GGCACTTCCA CTACTCGCCT GGCACAGGGC
    ATCGGGCGCA GCCAGAGAAC ACTCCGACAA CTCACAGCCA ACACCGGTCA CACAATACAT
    GTTCATTATC CTGGCAACAG GCAGCCAAAT CCACCTTTAA TCCTCCAGAG GTTGCTGGGG
    CCCTCAGCCG CTGCTGACAT CTTGCAACTA AGCAGCAGTC TTCCTCTGCA GAGTCGTGGC
    CGAGCACGTC TACTTGTTGG CAACGATGAT GTCCACATCA TAGCACGCTC TGATGATGAG
    ATGCTTGATG ATTTTTTCCA TGATCAGAGT ACAGCCACAA GTCAGGCTGG CACACTTTCC
    AGCATTCCAA CTGCGCTGAC CCGCTGGACA GAAGAGTGTA AGGTCTTGGA TGCTGAAAGC
    ATGCATGACT GTGTATCTGT GGTCAAAGTC CCAATCATCA ATCATTTGGA ATCTTTGCGT
    GATGAGGAGC TTGAGGAGCG CAGGGAAAAA AGAAGGAAAC AACTAGCAGA AGAGGAGGCC
    AAGATGGCAG AGAAATCCAA GGAGGAGAAG GAAGCAAAGG AGCAAAATTC ACAGGGTGCC
    AATTCAAAGC AGAATACCCC ATTTGAACTG AGCCTTGCAG ATCAAGATGG AAGTGGTCGT
    CCTGAAAATT TCCCAACTGC ACCATCTTCT GCAGAAGGAA CTCCAACAAG TCCTGAAATT
    GTTCGTTTAG ATCCCACCCT AAGGGCTACT GAGGCTACTC TGGCAGAGCT GACTCAGGGT
    CTGCCTGCTG ATACAGGCAC GGAGGATGTT GGGCAACAAC TCATGCCATC TCACCAAGAG
    GACTCTGGAC CAGGACGGCC AAGTGTAGAG GGGGATGCCA CACAAATGGA GCTATCGCCA
    GCACCCACTA TAACCTCCTT ATCACCAGAG CGAGCAGAGG ATTCAGATGC TTTGACAGCT
    GTTAGTAGCC AACTAGAGGG CTCGCCTATG GACACCAGCA GTCTTGCCTC TGGGCCCTTA
    GAGGATGGCG TAGGGGAGGC ACCTGGAATT GTTAATCCAG AGCAGCCTCT TGTGGTTGGC
    AGTAGCATTG CTGTTGGACA GCAACCAGTG GAACCACCGA CGACCTGTGA AACCCAACCA
    GTAGTTTCTC AACACAGTGA TAGCTCACAA ATTGCCCCTT CCTCTGAGTT GCCTTCCACA
    AGAGAAAGTG CTGTGACCTT GTTGGCTACA GATTCTAGTG GTATCCTTGA AGAACCTTTG
    CCTTCTACAA GCAGTGAAGA GGAAGACCCA TTAGCAGGCA TAAATCTTCC AGAGGGTGTG
    GATCCTTCTT TCCTAGCGGC TCTTCCTGAA GACATCCGCA GAGAGGTGCT GCAGAATCAG
    TTGGGCATAC GGCCGCCATC GCGACCTCCA CCCACTGCTG CCCCAGTTAT TCCTCCTCCT
    GTGGTTGCTA ATGCTGGTGT CACGGAGGTC AGCCCTGAGT TCTTGGCAGC GTTGCCACCA
    GCCATTCAGG AAGAGGTCCT AGCACAGCAG CGAGCAGAAC AGCAGAGGCG GGAACTTGCT
    CAGACCACTA CGTCTGATAC ACCCATGGAT CCTGTGACTT TCATTCAGAC GTTACCTTCT
    GATCTACGTC GTAGTGTGTT AGAAGACATG GAGGACAGTG TTTTAGCTGT GATGCCACCT
    GACATTGCTG CAGAGGCACA GGCTCTAAGG CGAGAGCAAG AAGCTCGGCA GAGGCAACTA
    ATGCATGAAC GACTGTTCGG GCACAGTAGC ACATCTGCAC TCTCTGCCAT CCTTAGGAGT
    CCTGCTTTCA CGAGTCGCTT GAGTGGAAAC CGAGGTGTTC AGTACACACG GTTGGCTGTA
    CAGAGAGGGA ACACTTTCCA AATGGGAAGC AGCAGTACAC ATACCAGACC AACCGGTAGT
    AATGTTGACA GCCTACTACG ATTACGAGGC CGACTATTAC TTGATCATGA AGCCCTCTCC
    TGTCTGCTTG TCCTACTGTT TGTTGATGAA CCCAAGCTAA ACACCAGTCG CTTGCACCGT
    GTTCTACGCA ACCTCTGCTA CCATGCACAA ACAAGGAACT GGGTGATTAG AAGCTTGTTG
    TCTATACTGC AGCGTAGCAG TGAAAGTGAG CTTTGTATAG AAAGCCCAAA GACTTCTATT
    GGGGGCACTG ATAAACGGGG TGCAAAAACA AGTCATGACA ACCGCCCTTT AGAATTTCTG
    CATAAGATGG AATCGAAGAA TTCCAACCAG CTCTCCTGGC TTTCTGTATC CATGGACGCT
    GCTCTTGGCT GCCGTACGAA CATCTTCCAA ATCCAGAGGT CCCTAGGACG CAAGCATACA
    GAAAAGCATT CAGCCTGTGG TTCTACTGTC CACATCCATC CCCAAGCAGC CCCTGTAGTG
    TGTAGACATG TGCTTGACAC ACTAATACAG CTAGCTAAGG TGTTTCCCAG TCATTTCACA
    CAGCAGCGAA CCAAAGAATG TAGTGTGGAA AACAGTGACA GCAGGGAGCG GAGTATAAAA
    CTTACTTGTA GTCCGTGTCT TGCACCATCT AGTAGTATTG GAGCAAGCAA CATCTCCACA
    GATTTCTGGG ACTTGCTAGT CAAGCTAGAC AACATGAATG TGAGCCGGAA GGGGAAAACG
    TCTATAAAAT CTGTGCCAGT GAGCTCTGGT TCTGAAGGCG AGAGCAATCA GTGCAGCCTG
    GAGGCCTCCC CCTTAGGACA GCTTATGAAT ATGCTGTCTC ATTCTGTTAT TCGCCGCAGT
    TCGCTGCTTA CTGAAAAGCT GCTGAGACTT CTCTCCCTTA TCTCTATTGC CCTTCCTGAT
    AATAAGGCTT CTGAAACAGC AGGCAATCAG AGCAATTCTA TTACCCCAGC ACCTGCACCT
    ACGCCTGCAA CTGCACCAGC CCCTGTGCCT GTACCTGCTG TAACAGTTCC AGTTGTTGTG
    AGCACAAACA TAGTGACAAC TGTTACAACC AAAAGTGGAA AATCTCCTGC AAAATCAAGT
    GAAGTCAGCA ATGCACCAGA TAAGATGGCA GCTTCTGGCC TTACAGAGAA ACAGCTGCAG
    TTGTCTGTTG AGGTTCTGAC ATCACACTCG TGTTCAGAAG AAGGACTGGA GGATGCCGCC
    AATGTTCTAC TTCAGCTTTC CAGAGGCGAC CCCAGTACAC GGGACACTGT ACTGAAACTT
    CTGCTTAGCG GAGCACATCA CTTGGGGTTT ACTCTCTGCA AACAGATAGG TACACTACTA
    GCAGAGCTCC GGGAATATAA CCTAGAGCAG CAGCGTCGTG CACAGTGTGA AGTTCTTTCT
    CCAGATGGTA TGCCTGAGGA GCAACCCCAC AGCACAAAGC CAAAAGGCAA AATGCAGAGC
    AGTGTTAGGT CTCTCCCCAC CCCACCTATG CGATCCAGGT TCGACATTGC AGAGAATGTG
    GTGATTGTGG CTTCCCAAAA GCGTCCACTG GGAGGAAGAG AGCTGCAGCT CCCCTCAATG
    TCTATGCTGA CGTCAAAGAC TTCCACACAA AAATTCTTTC TTCGAGTGCT GCAGGTTATC
    ATCCAGCTTC GCGATGACAC TCGCAGAGCC AACAAGAAGG CCAAGCAGAC CAGCAGATTA
    GGCACTGCCA GTCTGGCCTC GGCTAGCAGC ATCCAAGCAG CTGTGCAGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCTGCAA
    ACTGCAACGG CGGAGGGTTC TGCTCGCAGG GAGGAGCAGC CTATGGATGT TGACCAGCCT
    TCGCCTGCTA CACAGGAGCC TCAGTCAGTT GCTTCTGAAG GTTCTCAGGC TGCTGAAAAA
    AAAGAAGAGG AGAGGTTACC AGAGTTACCG CTGTTAAGTG AGCAGCTAAG TTTAGATGAG
    CTGTGGGACA TGTTGGGAGA GTGCCTGAAG GAACTTGAGG AATCTCATGA CCAGCATGCC
    GTATTAGTGT TACAGCCAGC CGTAGAAGCC TTTTTCCTTG TTCATGCAAC AGAGCGGGAG
    AGCAAGCCTC CTGTGCGGGA CACTCGGGAA AGCCAGTTAT CTCACATTAA GGATGAACCA
    CCTCCCCTGT CTCCTGCACC CCTCACTCCA GCCACACCTT CTTCCTTGGA TCCTTTCTTC
    TCTCGTGAAC CTTCATCTAT GCACATTTCC TCTAGTTTGC CTCCTGACAC TCAGAAGTTC
    CTAAGATTTG CTGAGACTCA TCGGACTGTA TTAAATCAGA TCCTTCGCCA GTCCACCACA
    CACCTAGCTG ATGGGCCCTT TGCTGTGCTT GTAGACTATA TCAGAGTCCT TGACTTTGAT
    GTTAAGCGCA AATATTTCCG ACAGGAGTTG GAGCGACTGG ATGAAGGGCT AAGAAAAGAG
    GATATGGCAG TGCATGTGCG GCGAGATCAT GTGTTTGAGG ATTCCTACCG GGAGCTGCAC
    CGCAAATCCC CTGAAGAAAT GAAGAATAGA TTGTACATAG TATTTGAAGG AGAAGAAGGG
    CAGGATGCAG GTGGGCTGCT TCGGGAGTGG TACATGATTA TCTCCAGGGA GATGTTCAAT
    CCCATGTATG CTTTGTTCCG CACTTCCCCA GGTGACCGCG TCACCTACAC TATTAATCCT
    TCCTCCCACT GTAACCCTAA CCACCTCAGC TACTTCAAGT TTGTGGGCCG GATAGTTGCC
    AAAGCGGTTT ATGACAACCG GCTACTGGAG TGTTACTTCA CTAGGTCCTT CTACAAGCAT
    ATATTGGGCA AGTCTGTCAG ATACACAGAC ATGGAGAGTG AAGATTATCA CTTCTATCAG
    GGTCTGGTGT ACTTGCTTGA AAATGATGTC TCCACACTGG GATATGACTT GACTTTCAGC
    ACTGAGGTTC AAGAATTTGG TGTCTGTGAA GTCCGAGACC TAAAACCAAA CGGTGCAAAC
    ATTCTGGTAA CAGAAGAAAA CAAAAAGGAG TATGTTCATC TTGTGTGCCA GATGAAGATG
    ACAGGTGCAA TACGTAAGCA ATTGGCTGCC TTCCTTGAGG GATTTTATGA GATAATCCCC
    AAACGTCTGA TCTCCATCTT CACTGAGCAG GAATTGGAAC TGCTTATCTC TGGCCTTCCC
    ACCATAGATA TTGATGACCT AAAATCAAAC ACTGAGTATC ACAAATACCA GTCTAACTCC
    ATCCAGATTC AGTGGTTCTG GAGAGCGTTG CGCTCATTCG ATCAAGCTGA CCGGGCAAAG
    TTCCTTCAGT TTGTTACTGG AACTTCAAAG GTCCCATTAC AGGGTTTTGC TGCGCTGGAA
    GGAATGAACG GCATCCAGAA ATTCCAAATC CACAGAGATG ATAGATCTAC AGACAGGCTG
    CCTTCTGCGC ACACGTGCTT CAACCAGTTG GACCTTCCAG CCTATGAGAG TTACGAGAAG
    CTACGTCACA TGCTGCTGTT AGCCATCCAG GAGTGCTCTG AGGGCTTTGG CCTTGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748029.1
    CDS671..13810
    Translation

    Target ORF information:

    RefSeq Version XM_031892169.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892169.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGCTGATG GATATGGGTT TCACAAGGGA GCATGCTCTG
    GAAGCTCTGC TTAACACGAG TACTATGGAG CAGGCCACTG AATATCTCCT CACCCACCCT
    CCACCTCTTA TTGGCGGAGT TGTACGGGAC TTAAGTATGT CAGAGGAAGA TCAGATGCTG
    AGAGCCATTG CCATGTCGCT GGGACAAGAT ATCCCTATGG AACAGAGGGC AGAACTGCCC
    GAGGAAACTG CTCGGCGGAA AGAAGAGGAG GAACGCAAGG CACGAGAAAA ACAAGAGGAA
    GAGGAAGCTA AATGCTTGGA AAAGTTTCAA GATGCAGAAC CCCTGGAACA GGATGAGCTT
    CAAGGATTCA CAGACACCAT GCTCCCTGGC TGCTTCCACT TGCTTGATGA GCTGCCAGAC
    ACAGTCTATA GAGTGTGTGA TCTTATAATG ACTGCCATAA AGCGCAATGG GGCAGACTAT
    CGAGACATGA TTCTAAAACA AGTGGTTAAC CAGGTATGGG AAGCCGCTGA TGTTCTTATT
    AAAGCTGCAC TGCCGCTGAC CACAAGTGAC ACAAAAACTG TGTCTGAGTG GATCAGCCAA
    ATGGCTACTC TTCCTCAAGC TTCTAACTTG GCCACTCGCA TCCTTTTGCT CACACTGCTG
    TTTGAGGAGC TAAAACTGCC ATGTGCTAGA GTTGTTGAAA CAAGTGGAAT ACTTAACGTA
    CTAATTAAGC TGCTTGAAGT GGTTCAGCCA TGTTTGCAGG CAGCCAAGGA GCAAAAGGAA
    GTTCAGACAC CAAAGTGGAT TACACCAGTC CTCCTCTTAA TAGATTTTTA TGAGAAGACC
    TCAATTTCTT CTAAAAGAAG AATGCAAATG AACAGGTACC TTCAAGCTAA TGGAAACAAC
    TGGCGATGGT TTGATGATCG GTCTGGCCGT TGGTGCAGCT ACAGTGCTAG TAACAACAGC
    ACTATTGATG CAGCCTGGAA AGCAGGAGAA ACATGTGTGC GATTTACAGC TGGCCGTCGA
    AGATATACTG TGCAGTTTAC CACAATGGTT CAGGTAAATG AAGAGACCGG CAATAGACGA
    CCTGTCATGC TGACTCTCTT GCGTGTTCCG AGACTTTTAA AGAGTGGAAA AAATGGAAAT
    GGACAGGAAC TTGAAAAGAC ACTTGAGGAG GCAAAGGAGG TTGAAGCAAA GCAAAAAGAC
    GAAAAAGTGC CTGAGCCCAT TCCAGTTCCT GAAACCTCTT CCACTGAGAA AGATGCACCA
    ACTGAAGAGC CCAAAGTTGG AGACATAGTA ATTCAGGGTC TGGCTGAAGA AATGGTGACC
    GTCCTTATAC GTTCCTGCGT CGGCATGCTT GGTGTTCCTG TTGATCCAGA TACTCTTCAT
    GCAACCTTGC GTCTTTGCCT GCGTCTCACA CGTGACCACA AATATGCCAT GATGTTTGCA
    GAGCTTAAGA GCACTCGAAT GATCCTCAGC TTAACTCAAA GCTCTGGATT CAATGGCTTT
    ACTCCCCTTG TGACGCTCCT ATTTAGGCAC ATCATAGAGG ATCCTTGCAC CCTCCAGCAC
    ACCATGGAAA AGGTTGTACG GTCTGCAGCA ACTAGTGGGG CAGGGAGTAC CACATCTGGG
    GTTGTGTCTG GAAGCCTGGG TTCGCGTGAA ATCAACTATA TACTTAGAGT ACTGGGTCCA
    GCAGCTTGTC GAAACCCTGA CATTTTCACT GAAGTGGCAA ACAGTTGCAT TCGCATTGCT
    CTCCCTGCAC CTCGTGGATC TGGAACTGCC TCTGATGATG AGTTTGAGAA CCTTCGGATC
    AAAGGTCCTA ATGCAGTTCA GTTGGTTAAA ACCACCCCTA TAAAGCCCTC CACCTTGCCA
    GTCATCCCTG ATACAATTAA GGAAGTAATC TATGACATGC TGAATGCACT GGCAGCTTAC
    CACGCCCCTG AGGAAGTATC TGAAAAGTCT GAGCAAAAAC CTGTTGGCAT GAACCAGGAA
    GTCAGCCAGA TCCTACAGGA CATGGGTGAT GAGGTTTTCC AGCAATATCG GGACTTAACC
    AGACAGGGAA GCGACTTTGA TGGTCAGACC AGTTTTGCCA TCAACGCGCG AGTCTTTACT
    GCTGACAGCA CAGCTGCCGA AACACCTCAG TCTGGTACAC CTCAAGGTGA AACTGCCTTA
    ACACCTGAAG AAAACCGAGA AGGGTTAAAG AAAGATAAGG AAGGAGACCG TAGTTCAGAA
    GATGGAAAGC AGAAGGCAAA AGGTAGTAAG CCTTTGCTGC CTACCTCTTC CATTCTTCGA
    CTACTTGCTG AACTTGTCAG GTCCTATGTC GGAATAGCCT CGCTGATCGC CACCTACAAT
    TACACTGCAG GACAATCTGA ACTAATTAAA GAGGATTGCA GTGTATTGGC GTTTGTGCTG
    GATCACCTGC TACCCCACAC ACATAATGCA GAGGACAAGG ACACACCAGC CCTAGCCCGC
    CTCTTCTTAG CCAGCCTTGC TGCAGCAGGA AGTGGCACTG ATGCCCAGGT TGCACTAGTG
    AATGAAGTAA AGGCAGCTCT TGGCAGAGCC CTAGCAATGG CTGAGAGCAC GGAGAAGCAT
    GCTAGGTTGC AGGCTGTGAT GTGCATTATC AGTACCATTA TGGAGTCATG TCCATCCACC
    TCCAGCTTCT ACAGCACAGC AACCACCAAA ACCCAGCACA ATGGCATGAA CAACATCATC
    AGACTCTTTT TGAAAAAGGG GCTGGTTAGT GATTTAGCCA GGGTCCCTCA CAGCCTTGAT
    TTATCCAGTC CAAATATGGC AAACACCGTG AATGCAGCAC TGAAGCCACT CGAGACACTG
    TCTCGCATTG TCAATCAACC CAGCAGTTTG TTTGGAAGCA AAGGGACTTC AGGAAAGAAT
    AAATCTGAGG CAGATACACA TGGAACAGCC CAGGATCCCA ACAGTAATCA GCCAGATACA
    GGTGAAACAG GAACAGAAGT GCAGGAAGAG GACCCAGATG CTTCTCATGC TGAAGTAGCT
    GATGGAGACA TCATGGATGG AGAGACAGAG ACTGATGCTG TGGTTATTGC TGGCCAGCCT
    GAAGTCCTTA CTACACAAGA AATGCAGGTA GAGAATGAGC TTGAAGATCT GATAGATGAA
    CTTCTTGAAA GGGATGGTGC TGCAGGTAAC CGGGTCGGAG AAGATGAATC ACAGGAAGAT
    GTGCTGATGG ATGAGCCTCC TACTAACCTC AGCCAAGCCT CCACTCTTCA GGCTAATCGA
    GAAGACTCTA TGAACATCTT AGACCCAGAG GATGAGGAAG AGCACACCCA AGAAGAGGAC
    AGCAGTGGCA GCAATGAAGA TGAAGATGAC AGCCAGGAAG AGGAGGAGGA AGAGGAAGAT
    GAGGACGATC AGGATGATGA GGAGGGTGAG GAAGGGGATG AGGATGATGA TGATGACTGT
    TCAGAGATGG AACTGGATGA GGATTATCCA GACATGAATG CCTCACCTCT TGTACGCTTT
    GAACGTTTTG ACAGAGAAGA TGATTTAATC ATAGAGTTTG ATAACATGTT CTCCAGTGCC
    ACTGAGATCC CTCCATCCCC TGGTAACATC CCTACTACAC ACCCCCTAAT GGTGCGACAC
    GCTGATCACG GCTCCTTGAC TCTTGGCAGT GGCACTTCCA CTACTCGCCT GGCACAGGGC
    ATCGGGCGCA GCCAGAGAAC ACTCCGACAA CTCACAGCCA ACACCGGTCA CACAATACAT
    GTTCATTATC CTGGCAACAG GCAGCCAAAT CCACCTTTAA TCCTCCAGAG GTTGCTGGGG
    CCCTCAGCCG CTGCTGACAT CTTGCAACTA AGCAGCAGTC TTCCTCTGCA GAGTCGTGGC
    CGAGCACGTC TACTTGTTGG CAACGATGAT GTCCACATCA TAGCACGCTC TGATGATGAG
    ATGCTTGATG ATTTTTTCCA TGATCAGAGT ACAGCCACAA GTCAGGCTGG CACACTTTCC
    AGCATTCCAA CTGCGCTGAC CCGCTGGACA GAAGAGTGTA AGGTCTTGGA TGCTGAAAGC
    ATGCATGACT GTGTATCTGT GGTCAAAGTC CCAATCATCA ATCATTTGGA ATCTTTGCGT
    GATGAGGAGC TTGAGGAGCG CAGGGAAAAA AGAAGGAAAC AACTAGCAGA AGAGGAGGCC
    AAGATGGCAG AGAAATCCAA GGAGGAGAAG GAAGCAAAGG AGCAAAATTC ACAGGGTGCC
    AATTCAAAGC AGAATACCCC ATTTGAACTG AGCCTTGCAG ATCAAGATGG AAGTGGTCGT
    CCTGAAAATT TCCCAACTGC ACCATCTTCT GCAGAAGGAA CTCCAACAAG TCCTGAAATT
    GTTCGTTTAG ATCCCACCCT AAGGGCTACT GAGGCTACTC TGGCAGAGCT GACTCAGGGT
    CTGCCTGCTG ATACAGGCAC GGAGGATGTT GGGCAACAAC TCATGCCATC TCACCAAGAG
    GACTCTGGAC CAGGACGGCC AAGTGTAGAG GGGGATGCCA CACAAATGGA GCTATCGCCA
    GCACCCACTA TAACCTCCTT ATCACCAGAG CGAGCAGAGG ATTCAGATGC TTTGACAGCT
    GTTAGTAGCC AACTAGAGGG CTCGCCTATG GACACCAGCA GTCTTGCCTC TGGGCCCTTA
    GAGGATGGCG TAGGGGAGGC ACCTGGAATT GTTAATCCAG AGCAGCCTCT TGTGGTTGGC
    AGTAGCATTG CTGTTGGACA GCAACCAGTG GAACCACCGA CGACCTGTGA AACCCAACCA
    GTAGTTTCTC AACACAGTGA TAGCTCACAA ATTGCCCCTT CCTCTGAGTT GCCTTCCACA
    AGAGAAAGTG CTGTGACCTT GTTGGCTACA GATTCTAGTG GTATCCTTGA AGAACCTTTG
    CCTTCTACAA GCAGTGAAGA GGAAGACCCA TTAGCAGGCA TAAATCTTCC AGAGGGTGTG
    GATCCTTCTT TCCTAGCGGC TCTTCCTGAA GACATCCGCA GAGAGGTGCT GCAGAATCAG
    TTGGGCATAC GGCCGCCATC GCGACCTCCA CCCACTGCTG CCCCAGTTAT TCCTCCTCCT
    GTGGTTGCTA ATGCTGGTGT CACGGAGGTC AGCCCTGAGT TCTTGGCAGC GTTGCCACCA
    GCCATTCAGG AAGAGGTCCT AGCACAGCAG CGAGCAGAAC AGCAGAGGCG GGAACTTGCT
    CAGACCACTA CGTCTGATAC ACCCATGGAT CCTGTGACTT TCATTCAGAC GTTACCTTCT
    GATCTACGTC GTAGTGTGTT AGAAGACATG GAGGACAGTG TTTTAGCTGT GATGCCACCT
    GACATTGCTG CAGAGGCACA GGCTCTAAGG CGAGAGCAAG AAGCTCGGCA GAGGCAACTA
    ATGCATGAAC GACTGTTCGG GCACAGTAGC ACATCTGCAC TCTCTGCCAT CCTTAGGAGT
    CCTGCTTTCA CGAGTCGCTT GAGTGGAAAC CGAGGTGTTC AGTACACACG GTTGGCTGTA
    CAGAGAGGGA ACACTTTCCA AATGGGAAGC AGCAGTACAC ATACCAGACC AACCGGTAGT
    AATGTTGACA GCCTACTACG ATTACGAGGC CGACTATTAC TTGATCATGA AGCCCTCTCC
    TGTCTGCTTG TCCTACTGTT TGTTGATGAA CCCAAGCTAA ACACCAGTCG CTTGCACCGT
    GTTCTACGCA ACCTCTGCTA CCATGCACAA ACAAGGAACT GGGTGATTAG AAGCTTGTTG
    TCTATACTGC AGCGTAGCAG TGAAAGTGAG CTTTGTATAG AAAGCCCAAA GACTTCTATT
    GGGGGCACTG ATAAACGGGG TGCAAAAACA AGTCATGACA ACCGCCCTTT AGAATTTCTG
    CATAAGATGG AATCGAAGAA TTCCAACCAG CTCTCCTGGC TTTCTGTATC CATGGACGCT
    GCTCTTGGCT GCCGTACGAA CATCTTCCAA ATCCAGAGGT CCCTAGGACG CAAGCATACA
    GAAAAGCATT CAGCCTGTGG TTCTACTGTC CACATCCATC CCCAAGCAGC CCCTGTAGTG
    TGTAGACATG TGCTTGACAC ACTAATACAG CTAGCTAAGG TGTTTCCCAG TCATTTCACA
    CAGCAGCGAA CCAAAGAATG TAGTGTGGAA AACAGTGACA GCAGGGAGCG GAGTATAAAA
    CTTACTTGTA GTCCGTGTCT TGCACCATCT AGTAGTATTG GAGCAAGCAA CATCTCCACA
    GATTTCTGGG ACTTGCTAGT CAAGCTAGAC AACATGAATG TGAGCCGGAA GGGGAAAACG
    TCTATAAAAT CTGTGCCAGT GAGCTCTGGT TCTGAAGGCG AGAGCAATCA GTGCAGCCTG
    GAGGCCTCCC CCTTAGGACA GCTTATGAAT ATGCTGTCTC ATTCTGTTAT TCGCCGCAGT
    TCGCTGCTTA CTGAAAAGCT GCTGAGACTT CTCTCCCTTA TCTCTATTGC CCTTCCTGAT
    AATAAGGCTT CTGAAACAGC AGGCAATCAG AGCAATTCTA TTACCCCAGC ACCTGCACCT
    ACGCCTGCAA CTGCACCAGC CCCTGTGCCT GTACCTGCTG TAACAGTTCC AGTTGTTGTG
    AGCACAAACA TAGTGACAAC TGTTACAACC AAAAGTGGAA AATCTCCTGC AAAATCAAGT
    GAAGTCAGCA ATGCACCAGA TAAGATGGCA GCTTCTGGCC TTACAGAGAA ACAGCTGCAG
    TTGTCTGTTG AGGTTCTGAC ATCACACTCG TGTTCAGAAG AAGGACTGGA GGATGCCGCC
    AATGTTCTAC TTCAGCTTTC CAGAGGCGAC CCCAGTACAC GGGACACTGT ACTGAAACTT
    CTGCTTAGCG GAGCACATCA CTTGGGGTTT ACTCTCTGCA AACAGATAGG TACACTACTA
    GCAGAGCTCC GGGAATATAA CCTAGAGCAG CAGCGTCGTG CACAGTGTGA AGTTCTTTCT
    CCAGATGGTA TGCCTGAGGA GCAACCCCAC AGCACAAAGC CAAAAGGCAA AATGCAGAGC
    AGTGTTAGGT CTCTCCCCAC CCCACCTATG CGATCCAGGT TCGACATTGC AGAGAATGTG
    GTGATTGTGG CTTCCCAAAA GCGTCCACTG GGAGGAAGAG AGCTGCAGCT CCCCTCAATG
    TCTATGCTGA CGTCAAAGAC TTCCACACAA AAATTCTTTC TTCGAGTGCT GCAGGTTATC
    ATCCAGCTTC GCGATGACAC TCGCAGAGCC AACAAGAAGG CCAAGCAGAC CAGCAGATTA
    GGCACTGCCA GTCTGGCCTC GGCTAGCAGC ATCCAAGCAG CTGTGCAGCA GCTGGAGGCT
    GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCTGCAA
    ACTGCAACGG CGGAGGGTTC TGCTCGCAGG GAGGAGCAGC CTATGGATGT TGACCAGCCT
    TCGCCTGCTA CACAGGAGCC TCAGTCAGTT GCTTCTGAAG GTTCTCAGGC TGCTGAAAAA
    AAAGAAGAGG AGAGGTTACC AGAGTTACCG CTGTTAAGTG AGCAGCTAAG TTTAGATGAG
    CTGTGGGACA TGTTGGGAGA GTGCCTGAAG GAACTTGAGG AATCTCATGA CCAGCATGCC
    GTATTAGTGT TACAGCCAGC CGTAGAAGCC TTTTTCCTTG TTCATGCAAC AGAGCGGGAG
    AGCAAGCCTC CTGTGCGGGA CACTCGGGAA AGCCAGTTAT CTCACATTAA GGATGAACCA
    CCTCCCCTGT CTCCTGCACC CCTCACTCCA GCCACACCTT CTTCCTTGGA TCCTTTCTTC
    TCTCGTGAAC CTTCATCTAT GCACATTTCC TCTAGTTTGC CTCCTGACAC TCAGAAGTTC
    CTAAGATTTG CTGAGACTCA TCGGACTGTA TTAAATCAGA TCCTTCGCCA GTCCACCACA
    CACCTAGCTG ATGGGCCCTT TGCTGTGCTT GTAGACTATA TCAGAGTCCT TGACTTTGAT
    GTTAAGCGCA AATATTTCCG ACAGGAGTTG GAGCGACTGG ATGAAGGGCT AAGAAAAGAG
    GATATGGCAG TGCATGTGCG GCGAGATCAT GTGTTTGAGG ATTCCTACCG GGAGCTGCAC
    CGCAAATCCC CTGAAGAAAT GAAGAATAGA TTGTACATAG TATTTGAAGG AGAAGAAGGG
    CAGGATGCAG GTGGGCTGCT TCGGGAGTGG TACATGATTA TCTCCAGGGA GATGTTCAAT
    CCCATGTATG CTTTGTTCCG CACTTCCCCA GGTGACCGCG TCACCTACAC TATTAATCCT
    TCCTCCCACT GTAACCCTAA CCACCTCAGC TACTTCAAGT TTGTGGGCCG GATAGTTGCC
    AAAGCGGTTT ATGACAACCG GCTACTGGAG TGTTACTTCA CTAGGTCCTT CTACAAGCAT
    ATATTGGGCA AGTCTGTCAG ATACACAGAC ATGGAGAGTG AAGATTATCA CTTCTATCAG
    GGTCTGGTGT ACTTGCTTGA AAATGATGTC TCCACACTGG GATATGACTT GACTTTCAGC
    ACTGAGGTTC AAGAATTTGG TGTCTGTGAA GTCCGAGACC TAAAACCAAA CGGTGCAAAC
    ATTCTGGTAA CAGAAGAAAA CAAAAAGGAG TATGTTCATC TTGTGTGCCA GATGAAGATG
    ACAGGTGCAA TACGTAAGCA ATTGGCTGCC TTCCTTGAGG GATTTTATGA GATAATCCCC
    AAACGTCTGA TCTCCATCTT CACTGAGCAG GAATTGGAAC TGCTTATCTC TGGCCTTCCC
    ACCATAGATA TTGATGACCT AAAATCAAAC ACTGAGTATC ACAAATACCA GTCTAACTCC
    ATCCAGATTC AGTGGTTCTG GAGAGCGTTG CGCTCATTCG ATCAAGCTGA CCGGGCAAAG
    TTCCTTCAGT TTGTTACTGG AACTTCAAAG GTCCCATTAC AGGGTTTTGC TGCGCTGGAA
    GGAATGAACG GCATCCAGAA ATTCCAAATC CACAGAGATG ATAGATCTAC AGACAGGCTG
    CCTTCTGCGC ACACGTGCTT CAACCAGTTG GACCTTCCAG CCTATGAGAG TTACGAGAAG
    CTACGTCACA TGCTGCTGTT AGCCATCCAG GAGTGCTCTG AGGGCTTTGG CCTTGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65590
    Clone ID Related Accession (Same CDS sequence) XM_031892170.1
    Accession Version XM_031892170.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGGTGCTG ATGGATATGG GTTTCACAAG GGAGCATGCT
    CTGGAAGCTC TGCTTAACAC GAGTACTATG GAGCAGGCCA CTGAATATCT CCTCACCCAC
    CCTCCACCTC TTATTGGCGG AGTTGTACGG GACTTAAGTA TGTCAGAGGA AGATCAGATG
    CTGAGAGCCA TTGCCATGTC GCTGGGACAA GATATCCCTA TGGAACAGAG GGCAGAACTG
    CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG GAGGAACGCA AGGCACGAGA AAAACAAGAG
    GAAGAGGAAG CTAAATGCTT GGAAAAGTTT CAAGATGCAG AACCCCTGGA ACAGGATGAG
    CTTCAAGGAT TCACAGACAC CATGCTCCCT GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA
    GACACAGTCT ATAGAGTGTG TGATCTTATA ATGACTGCCA TAAAGCGCAA TGGGGCAGAC
    TATCGAGACA TGATTCTAAA ACAAGTGGTT AACCAGGTAT GGGAAGCCGC TGATGTTCTT
    ATTAAAGCTG CACTGCCGCT GACCACAAGT GACACAAAAA CTGTGTCTGA GTGGATCAGC
    CAAATGGCTA CTCTTCCTCA AGCTTCTAAC TTGGCCACTC GCATCCTTTT GCTCACACTG
    CTGTTTGAGG AGCTAAAACT GCCATGTGCT AGAGTTGTTG AAACAAGTGG AATACTTAAC
    GTACTAATTA AGCTGCTTGA AGTGGTTCAG CCATGTTTGC AGGCAGCCAA GGAGCAAAAG
    GAAGTTCAGA CACCAAAGTG GATTACACCA GTCCTCCTCT TAATAGATTT TTATGAGAAG
    ACCTCAATTT CTTCTAAAAG AAGAATGCAA ATGAACAGGT ACCTTCAAGC TAATGGAAAC
    AACTGGCGAT GGTTTGATGA TCGGTCTGGC CGTTGGTGCA GCTACAGTGC TAGTAACAAC
    AGCACTATTG ATGCAGCCTG GAAAGCAGGA GAAACATGTG TGCGATTTAC AGCTGGCCGT
    CGAAGATATA CTGTGCAGTT TACCACAATG GTTCAGGTAA ATGAAGAGAC CGGCAATAGA
    CGACCTGTCA TGCTGACTCT CTTGCGTGTT CCGAGACTTT TAAAGAGTGG AAAAAATGGA
    AATGGACAGG AACTTGAAAA GACACTTGAG GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA
    GACGAAAAAG TGCCTGAGCC CATTCCAGTT CCTGAAACCT CTTCCACTGA GAAAGATGCA
    CCAACTGAAG AGCCCAAAGT TGGAGACATA GTAATTCAGG GTCTGGCTGA AGAAATGGTG
    ACCGTCCTTA TACGTTCCTG CGTCGGCATG CTTGGTGTTC CTGTTGATCC AGATACTCTT
    CATGCAACCT TGCGTCTTTG CCTGCGTCTC ACACGTGACC ACAAATATGC CATGATGTTT
    GCAGAGCTTA AGAGCACTCG AATGATCCTC AGCTTAACTC AAAGCTCTGG ATTCAATGGC
    TTTACTCCCC TTGTGACGCT CCTATTTAGG CACATCATAG AGGATCCTTG CACCCTCCAG
    CACACCATGG AAAAGGTTGT ACGGTCTGCA GCAACTAGTG GGGCAGGGAG TACCACATCT
    GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT GAAATCAACT ATATACTTAG AGTACTGGGT
    CCAGCAGCTT GTCGAAACCC TGACATTTTC ACTGAAGTGG CAAACAGTTG CATTCGCATT
    GCTCTCCCTG CACCTCGTGG ATCTGGAACT GCCTCTGATG ATGAGTTTGA GAACCTTCGG
    ATCAAAGGTC CTAATGCAGT TCAGTTGGTT AAAACCACCC CTATAAAGCC CTCCACCTTG
    CCAGTCATCC CTGATACAAT TAAGGAAGTA ATCTATGACA TGCTGAATGC ACTGGCAGCT
    TACCACGCCC CTGAGGAAGT ATCTGAAAAG TCTGAGCAAA AACCTGTTGG CATGAACCAG
    GAAGTCAGCC AGATCCTACA GGACATGGGT GATGAGGTTT TCCAGCAATA TCGGGACTTA
    ACCAGACAGG GAAGCGACTT TGATGGTCAG ACCAGTTTTG CCATCAACGC GCGAGTCTTT
    ACTGCTGACA GCACAGCTGC CGAAACACCT CAGTCTGGTA CACCTCAAGG TGAAACTGCC
    TTAACACCTG AAGAAAACCG AGAAGGGTTA AAGAAAGATA AGGAAGGAGA CCGTAGTTCA
    GAAGATGGAA AGCAGAAGGC AAAAGGTAGT AAGCCTTTGC TGCCTACCTC TTCCATTCTT
    CGACTACTTG CTGAACTTGT CAGGTCCTAT GTCGGAATAG CCTCGCTGAT CGCCACCTAC
    AATTACACTG CAGGACAATC TGAACTAATT AAAGAGGATT GCAGTGTATT GGCGTTTGTG
    CTGGATCACC TGCTACCCCA CACACATAAT GCAGAGGACA AGGACACACC AGCCCTAGCC
    CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA GGAAGTGGCA CTGATGCCCA GGTTGCACTA
    GTGAATGAAG TAAAGGCAGC TCTTGGCAGA GCCCTAGCAA TGGCTGAGAG CACGGAGAAG
    CATGCTAGGT TGCAGGCTGT GATGTGCATT ATCAGTACCA TTATGGAGTC ATGTCCATCC
    ACCTCCAGCT TCTACAGCAC AGCAACCACC AAAACCCAGC ACAATGGCAT GAACAACATC
    ATCAGACTCT TTTTGAAAAA GGGGCTGGTT AGTGATTTAG CCAGGGTCCC TCACAGCCTT
    GATTTATCCA GTCCAAATAT GGCAAACACC GTGAATGCAG CACTGAAGCC ACTCGAGACA
    CTGTCTCGCA TTGTCAATCA ACCCAGCAGT TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG
    AATAAATCTG AGGCAGATAC ACATGGAACA GCCCAGGATC CCAACAGTAA TCAGCCAGAT
    ACAGGTGAAA CAGGAACAGA AGTGCAGGAA GAGGACCCAG ATGCTTCTCA TGCTGAAGTA
    GCTGATGGAG ACATCATGGA TGGAGAGACA GAGACTGATG CTGTGGTTAT TGCTGGCCAG
    CCTGAAGTCC TTACTACACA AGAAATGCAG GTAGAGAATG AGCTTGAAGA TCTGATAGAT
    GAACTTCTTG AAAGGGATGG TGCTGCAGGT AACCGGGTCG GAGAAGATGA ATCACAGGAA
    GATGTGCTGA TGGATGAGCC TCCTACTAAC CTCAGCCAAG CCTCCACTCT TCAGGCTAAT
    CGAGAAGACT CTATGAACAT CTTAGACCCA GAGGATGAGG AAGAGCACAC CCAAGAAGAG
    GACAGCAGTG GCAGCAATGA AGATGAAGAT GACAGCCAGG AAGAGGAGGA GGAAGAGGAA
    GATGAGGACG ATCAGGATGA TGAGGAGGGT GAGGAAGGGG ATGAGGATGA TGATGATGAC
    TGTTCAGAGA TGGAACTGGA TGAGGATTAT CCAGACATGA ATGCCTCACC TCTTGTACGC
    TTTGAACGTT TTGACAGAGA AGATGATTTA ATCATAGAGT TTGATAACAT GTTCTCCAGT
    GCCACTGAGA TCCCTCCATC CCCTGGTAAC ATCCCTACTA CACACCCCCT AATGGTGCGA
    CACGCTGATC ACGGCTCCTT GACTCTTGGC AGTGGCACTT CCACTACTCG CCTGGCACAG
    GGCATCGGGC GCAGCCAGAG AACACTCCGA CAACTCACAG CCAACACCGG TCACACAATA
    CATGTTCATT ATCCTGGCAA CAGGCAGCCA AATCCACCTT TAATCCTCCA GAGGTTGCTG
    GGGCCCTCAG CCGCTGCTGA CATCTTGCAA CTAAGCAGCA GTCTTCCTCT GCAGAGTCGT
    GGCCGAGCAC GTCTACTTGT TGGCAACGAT GATGTCCACA TCATAGCACG CTCTGATGAT
    GAGATGCTTG ATGATTTTTT CCATGATCAG AGTACAGCCA CAAGTCAGGC TGGCACACTT
    TCCAGCATTC CAACTGCGCT GACCCGCTGG ACAGAAGAGT GTAAGGTCTT GGATGCTGAA
    AGCATGCATG ACTGTGTATC TGTGGTCAAA GTCCCAATCA TCAATCATTT GGAATCTTTG
    CGTGATGAGG AGCTTGAGGA GCGCAGGGAA AAAAGAAGGA AACAACTAGC AGAAGAGGAG
    GCCAAGATGG CAGAGAAATC CAAGGAGGAG AAGGAAGCAA AGGAGCAAAA TTCACAGGGT
    GCCAATTCAA AGCAGAATAC CCCATTTGAA CTGAGCCTTG CAGATCAAGA TGGAAGTGGT
    CGTCCTGAAA ATTTCCCAAC TGCACCATCT TCTGCAGAAG GAACTCCAAC AAGTCCTGAA
    ATTGTTCGTT TAGATCCCAC CCTAAGGGCT ACTGAGGCTA CTCTGGCAGA GCTGACTCAG
    GGTCTGCCTG CTGATACAGG CACGGAGGAT GTTGGGCAAC AACTCATGCC ATCTCACCAA
    GAGGACTCTG GACCAGGACG GCCAAGTGTA GAGGGGGATG CCACACAAAT GGAGCTATCG
    CCAGCACCCA CTATAACCTC CTTATCACCA GAGCGAGCAG AGGATTCAGA TGCTTTGACA
    GCTGTTAGTA GCCAACTAGA GGGCTCGCCT ATGGACACCA GCAGTCTTGC CTCTGGGCCC
    TTAGAGGATG GCGTAGGGGA GGCACCTGGA ATTGTTAATC CAGAGCAGCC TCTTGTGGTT
    GGCAGTAGCA TTGCTGTTGG ACAGCAACCA GTGGAACCAC CGACGACCTG TGAAACCCAA
    CCAGTAGTTT CTCAACACAG TGATAGCTCA CAAATTGCCC CTTCCTCTGA GTTGCCTTCC
    ACAAGAGAAA GTGCTGTGAC CTTGTTGGCT ACAGATTCTA GTGGTATCCT TGAAGAACCT
    TTGCCTTCTA CAAGCAGTGA AGAGGAAGAC CCATTAGCAG GCATAAATCT TCCAGAGGGT
    GTGGATCCTT CTTTCCTAGC GGCTCTTCCT GAAGACATCC GCAGAGAGGT GCTGCAGAAT
    CAGTTGGGCA TACGGCCGCC ATCGCGACCT CCACCCACTG CTGCCCCAGT TATTCCTCCT
    CCTGTGGTTG CTAATGCTGG TGTCACGGAG GTCAGCCCTG AGTTCTTGGC AGCGTTGCCA
    CCAGCCATTC AGGAAGAGGT CCTAGCACAG CAGCGAGCAG AACAGCAGAG GCGGGAACTT
    GCTCAGACCA CTACGTCTGA TACACCCATG GATCCTGTGA CTTTCATTCA GACGTTACCT
    TCTGATCTAC GTCGTAGTGT GTTAGAAGAC ATGGAGGACA GTGTTTTAGC TGTGATGCCA
    CCTGACATTG CTGCAGAGGC ACAGGCTCTA AGGCGAGAGC AAGAAGCTCG GCAGAGGCAA
    CTAATGCATG AACGACTGTT CGGGCACAGT AGCACATCTG CACTCTCTGC CATCCTTAGG
    AGTCCTGCTT TCACGAGTCG CTTGAGTGGA AACCGAGGTG TTCAGTACAC ACGGTTGGCT
    GTACAGAGAG GGAACACTTT CCAAATGGGA AGCAGCAGTA CACATACCAG ACCAACCGGT
    AGTAATGTTG ACAGCCTACT ACGATTACGA GGCCGACTAT TACTTGATCA TGAAGCCCTC
    TCCTGTCTGC TTGTCCTACT GTTTGTTGAT GAACCCAAGC TAAACACCAG TCGCTTGCAC
    CGTGTTCTAC GCAACCTCTG CTACCATGCA CAAACAAGGA ACTGGGTGAT TAGAAGCTTG
    TTGTCTATAC TGCAGCGTAG CAGTGAAAGT GAGCTTTGTA TAGAAAGCCC AAAGACTTCT
    ATTGGGGGCA CTGATAAACG GGGTGCAAAA ACAAGTCATG ACAACCGCCC TTTAGAATTT
    CTGCATAAGA TGGAATCGAA GAATTCCAAC CAGCTCTCCT GGCTTTCTGT ATCCATGGAC
    GCTGCTCTTG GCTGCCGTAC GAACATCTTC CAAATCCAGA GGTCCCTAGG ACGCAAGCAT
    ACAGAAAAGC ATTCAGCCTG TGGTTCTACT GTCCACATCC ATCCCCAAGC AGCCCCTGTA
    GTGTGTAGAC ATGTGCTTGA CACACTAATA CAGCTAGCTA AGGTGTTTCC CAGTCATTTC
    ACACAGCAGC GAACCAAAGA ATGTAGTGTG GAAAACAGTG ACAGCAGGGA GCGGAGTATA
    AAACTTACTT GTAGTCCGTG TCTTGCACCA TCTAGTAGTA TTGGAGCAAG CAACATCTCC
    ACAGATTTCT GGGACTTGCT AGTCAAGCTA GACAACATGA ATGTGAGCCG GAAGGGGAAA
    ACGTCTATAA AATCTGTGCC AGTGAGCTCT GGTTCTGAAG GCGAGAGCAA TCAGTGCAGC
    CTGGAGGCCT CCCCCTTAGG ACAGCTTATG AATATGCTGT CTCATTCTGT TATTCGCCGC
    AGTTCGCTGC TTACTGAAAA GCTGCTGAGA CTTCTCTCCC TTATCTCTAT TGCCCTTCCT
    GATAATAAGG CTTCTGAAAC AGCAGGCAAT CAGAGCAATT CTATTACCCC AGCACCTGCA
    CCTACGCCTG CAACTGCACC AGCCCCTGTG CCTGTACCTG CTGTAACAGT TCCAGTTGTT
    GTGAGCACAA ACATAGTGAC AACTGTTACA ACCAAAAGTG GAAAATCTCC TGCAAAATCA
    AGTGAAGTCA GCAATGCACC AGATAAGATG GCAGCTTCTG GCCTTACAGA GAAACAGCTG
    CAGTTGTCTG TTGAGGTTCT GACATCACAC TCGTGTTCAG AAGAAGGACT GGAGGATGCC
    GCCAATGTTC TACTTCAGCT TTCCAGAGGC GACCCCAGTA CACGGGACAC TGTACTGAAA
    CTTCTGCTTA GCGGAGCACA TCACTTGGGG TTTACTCTCT GCAAACAGAT AGGTACACTA
    CTAGCAGAGC TCCGGGAATA TAACCTAGAG CAGCAGCGTC GTGCACAGTG TGAAGTTCTT
    TCTCCAGATG GTATGCCTGA GGAGCAACCC CACAGCACAA AGCCAAAAGG CAAAATGCAG
    AGCAGGTCTC TCCCCACCCC ACCTATGCGA TCCAGGTTCG ACATTGCAGA GAATGTGGTG
    ATTGTGGCTT CCCAAAAGCG TCCACTGGGA GGAAGAGAGC TGCAGCTCCC CTCAATGTCT
    ATGCTGACGT CAAAGACTTC CACACAAAAA TTCTTTCTTC GAGTGCTGCA GGTTATCATC
    CAGCTTCGCG ATGACACTCG CAGAGCCAAC AAGAAGGCCA AGCAGACCAG CAGATTAGGC
    ACTGCCAGTC TGGCCTCGGC TAGCAGCATC CAAGCAGCTG TGCAGCAGCT GGAGGCTGAG
    GCTGATGCCA TTATACAAAT GGTACGTGAG GGTCAAAGGG CGCGGAGACA GCTGCAAACT
    GCAACGGCGG AGGGTTCTGC TCGCAGGGAG GAGCAGCCTA TGGATGTTGA CCAGCCTTCG
    CCTGCTACAC AGGAGCCTCA GTCAGTTGCT TCTGAAGGTT CTCAGGCTGC TGAAAAAAAA
    GAAGAGGAGA GGTTACCAGA GTTACCGCTG TTAAGTGAGC AGCTAAGTTT AGATGAGCTG
    TGGGACATGT TGGGAGAGTG CCTGAAGGAA CTTGAGGAAT CTCATGACCA GCATGCCGTA
    TTAGTGTTAC AGCCAGCCGT AGAAGCCTTT TTCCTTGTTC ATGCAACAGA GCGGGAGAGC
    AAGCCTCCTG TGCGGGACAC TCGGGAAAGC CAGTTATCTC ACATTAAGGA TGAACCACCT
    CCCCTGTCTC CTGCACCCCT CACTCCAGCC ACACCTTCTT CCTTGGATCC TTTCTTCTCT
    CGTGAACCTT CATCTATGCA CATTTCCTCT AGTTTGCCTC CTGACACTCA GAAGTTCCTA
    AGATTTGCTG AGACTCATCG GACTGTATTA AATCAGATCC TTCGCCAGTC CACCACACAC
    CTAGCTGATG GGCCCTTTGC TGTGCTTGTA GACTATATCA GAGTCCTTGA CTTTGATGTT
    AAGCGCAAAT ATTTCCGACA GGAGTTGGAG CGACTGGATG AAGGGCTAAG AAAAGAGGAT
    ATGGCAGTGC ATGTGCGGCG AGATCATGTG TTTGAGGATT CCTACCGGGA GCTGCACCGC
    AAATCCCCTG AAGAAATGAA GAATAGATTG TACATAGTAT TTGAAGGAGA AGAAGGGCAG
    GATGCAGGTG GGCTGCTTCG GGAGTGGTAC ATGATTATCT CCAGGGAGAT GTTCAATCCC
    ATGTATGCTT TGTTCCGCAC TTCCCCAGGT GACCGCGTCA CCTACACTAT TAATCCTTCC
    TCCCACTGTA ACCCTAACCA CCTCAGCTAC TTCAAGTTTG TGGGCCGGAT AGTTGCCAAA
    GCGGTTTATG ACAACCGGCT ACTGGAGTGT TACTTCACTA GGTCCTTCTA CAAGCATATA
    TTGGGCAAGT CTGTCAGATA CACAGACATG GAGAGTGAAG ATTATCACTT CTATCAGGGT
    CTGGTGTACT TGCTTGAAAA TGATGTCTCC ACACTGGGAT ATGACTTGAC TTTCAGCACT
    GAGGTTCAAG AATTTGGTGT CTGTGAAGTC CGAGACCTAA AACCAAACGG TGCAAACATT
    CTGGTAACAG AAGAAAACAA AAAGGAGTAT GTTCATCTTG TGTGCCAGAT GAAGATGACA
    GGTGCAATAC GTAAGCAATT GGCTGCCTTC CTTGAGGGAT TTTATGAGAT AATCCCCAAA
    CGTCTGATCT CCATCTTCAC TGAGCAGGAA TTGGAACTGC TTATCTCTGG CCTTCCCACC
    ATAGATATTG ATGACCTAAA ATCAAACACT GAGTATCACA AATACCAGTC TAACTCCATC
    CAGATTCAGT GGTTCTGGAG AGCGTTGCGC TCATTCGATC AAGCTGACCG GGCAAAGTTC
    CTTCAGTTTG TTACTGGAAC TTCAAAGGTC CCATTACAGG GTTTTGCTGC GCTGGAAGGA
    ATGAACGGCA TCCAGAAATT CCAAATCCAC AGAGATGATA GATCTACAGA CAGGCTGCCT
    TCTGCGCACA CGTGCTTCAA CCAGTTGGAC CTTCCAGCCT ATGAGAGTTA CGAGAAGCTA
    CGTCACATGC TGCTGTTAGC CATCCAGGAG TGCTCTGAGG GCTTTGGCCT TGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748030.1
    CDS671..13807
    Translation

    Target ORF information:

    RefSeq Version XM_031892170.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892170.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGGTGCTG ATGGATATGG GTTTCACAAG GGAGCATGCT
    CTGGAAGCTC TGCTTAACAC GAGTACTATG GAGCAGGCCA CTGAATATCT CCTCACCCAC
    CCTCCACCTC TTATTGGCGG AGTTGTACGG GACTTAAGTA TGTCAGAGGA AGATCAGATG
    CTGAGAGCCA TTGCCATGTC GCTGGGACAA GATATCCCTA TGGAACAGAG GGCAGAACTG
    CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG GAGGAACGCA AGGCACGAGA AAAACAAGAG
    GAAGAGGAAG CTAAATGCTT GGAAAAGTTT CAAGATGCAG AACCCCTGGA ACAGGATGAG
    CTTCAAGGAT TCACAGACAC CATGCTCCCT GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA
    GACACAGTCT ATAGAGTGTG TGATCTTATA ATGACTGCCA TAAAGCGCAA TGGGGCAGAC
    TATCGAGACA TGATTCTAAA ACAAGTGGTT AACCAGGTAT GGGAAGCCGC TGATGTTCTT
    ATTAAAGCTG CACTGCCGCT GACCACAAGT GACACAAAAA CTGTGTCTGA GTGGATCAGC
    CAAATGGCTA CTCTTCCTCA AGCTTCTAAC TTGGCCACTC GCATCCTTTT GCTCACACTG
    CTGTTTGAGG AGCTAAAACT GCCATGTGCT AGAGTTGTTG AAACAAGTGG AATACTTAAC
    GTACTAATTA AGCTGCTTGA AGTGGTTCAG CCATGTTTGC AGGCAGCCAA GGAGCAAAAG
    GAAGTTCAGA CACCAAAGTG GATTACACCA GTCCTCCTCT TAATAGATTT TTATGAGAAG
    ACCTCAATTT CTTCTAAAAG AAGAATGCAA ATGAACAGGT ACCTTCAAGC TAATGGAAAC
    AACTGGCGAT GGTTTGATGA TCGGTCTGGC CGTTGGTGCA GCTACAGTGC TAGTAACAAC
    AGCACTATTG ATGCAGCCTG GAAAGCAGGA GAAACATGTG TGCGATTTAC AGCTGGCCGT
    CGAAGATATA CTGTGCAGTT TACCACAATG GTTCAGGTAA ATGAAGAGAC CGGCAATAGA
    CGACCTGTCA TGCTGACTCT CTTGCGTGTT CCGAGACTTT TAAAGAGTGG AAAAAATGGA
    AATGGACAGG AACTTGAAAA GACACTTGAG GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA
    GACGAAAAAG TGCCTGAGCC CATTCCAGTT CCTGAAACCT CTTCCACTGA GAAAGATGCA
    CCAACTGAAG AGCCCAAAGT TGGAGACATA GTAATTCAGG GTCTGGCTGA AGAAATGGTG
    ACCGTCCTTA TACGTTCCTG CGTCGGCATG CTTGGTGTTC CTGTTGATCC AGATACTCTT
    CATGCAACCT TGCGTCTTTG CCTGCGTCTC ACACGTGACC ACAAATATGC CATGATGTTT
    GCAGAGCTTA AGAGCACTCG AATGATCCTC AGCTTAACTC AAAGCTCTGG ATTCAATGGC
    TTTACTCCCC TTGTGACGCT CCTATTTAGG CACATCATAG AGGATCCTTG CACCCTCCAG
    CACACCATGG AAAAGGTTGT ACGGTCTGCA GCAACTAGTG GGGCAGGGAG TACCACATCT
    GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT GAAATCAACT ATATACTTAG AGTACTGGGT
    CCAGCAGCTT GTCGAAACCC TGACATTTTC ACTGAAGTGG CAAACAGTTG CATTCGCATT
    GCTCTCCCTG CACCTCGTGG ATCTGGAACT GCCTCTGATG ATGAGTTTGA GAACCTTCGG
    ATCAAAGGTC CTAATGCAGT TCAGTTGGTT AAAACCACCC CTATAAAGCC CTCCACCTTG
    CCAGTCATCC CTGATACAAT TAAGGAAGTA ATCTATGACA TGCTGAATGC ACTGGCAGCT
    TACCACGCCC CTGAGGAAGT ATCTGAAAAG TCTGAGCAAA AACCTGTTGG CATGAACCAG
    GAAGTCAGCC AGATCCTACA GGACATGGGT GATGAGGTTT TCCAGCAATA TCGGGACTTA
    ACCAGACAGG GAAGCGACTT TGATGGTCAG ACCAGTTTTG CCATCAACGC GCGAGTCTTT
    ACTGCTGACA GCACAGCTGC CGAAACACCT CAGTCTGGTA CACCTCAAGG TGAAACTGCC
    TTAACACCTG AAGAAAACCG AGAAGGGTTA AAGAAAGATA AGGAAGGAGA CCGTAGTTCA
    GAAGATGGAA AGCAGAAGGC AAAAGGTAGT AAGCCTTTGC TGCCTACCTC TTCCATTCTT
    CGACTACTTG CTGAACTTGT CAGGTCCTAT GTCGGAATAG CCTCGCTGAT CGCCACCTAC
    AATTACACTG CAGGACAATC TGAACTAATT AAAGAGGATT GCAGTGTATT GGCGTTTGTG
    CTGGATCACC TGCTACCCCA CACACATAAT GCAGAGGACA AGGACACACC AGCCCTAGCC
    CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA GGAAGTGGCA CTGATGCCCA GGTTGCACTA
    GTGAATGAAG TAAAGGCAGC TCTTGGCAGA GCCCTAGCAA TGGCTGAGAG CACGGAGAAG
    CATGCTAGGT TGCAGGCTGT GATGTGCATT ATCAGTACCA TTATGGAGTC ATGTCCATCC
    ACCTCCAGCT TCTACAGCAC AGCAACCACC AAAACCCAGC ACAATGGCAT GAACAACATC
    ATCAGACTCT TTTTGAAAAA GGGGCTGGTT AGTGATTTAG CCAGGGTCCC TCACAGCCTT
    GATTTATCCA GTCCAAATAT GGCAAACACC GTGAATGCAG CACTGAAGCC ACTCGAGACA
    CTGTCTCGCA TTGTCAATCA ACCCAGCAGT TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG
    AATAAATCTG AGGCAGATAC ACATGGAACA GCCCAGGATC CCAACAGTAA TCAGCCAGAT
    ACAGGTGAAA CAGGAACAGA AGTGCAGGAA GAGGACCCAG ATGCTTCTCA TGCTGAAGTA
    GCTGATGGAG ACATCATGGA TGGAGAGACA GAGACTGATG CTGTGGTTAT TGCTGGCCAG
    CCTGAAGTCC TTACTACACA AGAAATGCAG GTAGAGAATG AGCTTGAAGA TCTGATAGAT
    GAACTTCTTG AAAGGGATGG TGCTGCAGGT AACCGGGTCG GAGAAGATGA ATCACAGGAA
    GATGTGCTGA TGGATGAGCC TCCTACTAAC CTCAGCCAAG CCTCCACTCT TCAGGCTAAT
    CGAGAAGACT CTATGAACAT CTTAGACCCA GAGGATGAGG AAGAGCACAC CCAAGAAGAG
    GACAGCAGTG GCAGCAATGA AGATGAAGAT GACAGCCAGG AAGAGGAGGA GGAAGAGGAA
    GATGAGGACG ATCAGGATGA TGAGGAGGGT GAGGAAGGGG ATGAGGATGA TGATGATGAC
    TGTTCAGAGA TGGAACTGGA TGAGGATTAT CCAGACATGA ATGCCTCACC TCTTGTACGC
    TTTGAACGTT TTGACAGAGA AGATGATTTA ATCATAGAGT TTGATAACAT GTTCTCCAGT
    GCCACTGAGA TCCCTCCATC CCCTGGTAAC ATCCCTACTA CACACCCCCT AATGGTGCGA
    CACGCTGATC ACGGCTCCTT GACTCTTGGC AGTGGCACTT CCACTACTCG CCTGGCACAG
    GGCATCGGGC GCAGCCAGAG AACACTCCGA CAACTCACAG CCAACACCGG TCACACAATA
    CATGTTCATT ATCCTGGCAA CAGGCAGCCA AATCCACCTT TAATCCTCCA GAGGTTGCTG
    GGGCCCTCAG CCGCTGCTGA CATCTTGCAA CTAAGCAGCA GTCTTCCTCT GCAGAGTCGT
    GGCCGAGCAC GTCTACTTGT TGGCAACGAT GATGTCCACA TCATAGCACG CTCTGATGAT
    GAGATGCTTG ATGATTTTTT CCATGATCAG AGTACAGCCA CAAGTCAGGC TGGCACACTT
    TCCAGCATTC CAACTGCGCT GACCCGCTGG ACAGAAGAGT GTAAGGTCTT GGATGCTGAA
    AGCATGCATG ACTGTGTATC TGTGGTCAAA GTCCCAATCA TCAATCATTT GGAATCTTTG
    CGTGATGAGG AGCTTGAGGA GCGCAGGGAA AAAAGAAGGA AACAACTAGC AGAAGAGGAG
    GCCAAGATGG CAGAGAAATC CAAGGAGGAG AAGGAAGCAA AGGAGCAAAA TTCACAGGGT
    GCCAATTCAA AGCAGAATAC CCCATTTGAA CTGAGCCTTG CAGATCAAGA TGGAAGTGGT
    CGTCCTGAAA ATTTCCCAAC TGCACCATCT TCTGCAGAAG GAACTCCAAC AAGTCCTGAA
    ATTGTTCGTT TAGATCCCAC CCTAAGGGCT ACTGAGGCTA CTCTGGCAGA GCTGACTCAG
    GGTCTGCCTG CTGATACAGG CACGGAGGAT GTTGGGCAAC AACTCATGCC ATCTCACCAA
    GAGGACTCTG GACCAGGACG GCCAAGTGTA GAGGGGGATG CCACACAAAT GGAGCTATCG
    CCAGCACCCA CTATAACCTC CTTATCACCA GAGCGAGCAG AGGATTCAGA TGCTTTGACA
    GCTGTTAGTA GCCAACTAGA GGGCTCGCCT ATGGACACCA GCAGTCTTGC CTCTGGGCCC
    TTAGAGGATG GCGTAGGGGA GGCACCTGGA ATTGTTAATC CAGAGCAGCC TCTTGTGGTT
    GGCAGTAGCA TTGCTGTTGG ACAGCAACCA GTGGAACCAC CGACGACCTG TGAAACCCAA
    CCAGTAGTTT CTCAACACAG TGATAGCTCA CAAATTGCCC CTTCCTCTGA GTTGCCTTCC
    ACAAGAGAAA GTGCTGTGAC CTTGTTGGCT ACAGATTCTA GTGGTATCCT TGAAGAACCT
    TTGCCTTCTA CAAGCAGTGA AGAGGAAGAC CCATTAGCAG GCATAAATCT TCCAGAGGGT
    GTGGATCCTT CTTTCCTAGC GGCTCTTCCT GAAGACATCC GCAGAGAGGT GCTGCAGAAT
    CAGTTGGGCA TACGGCCGCC ATCGCGACCT CCACCCACTG CTGCCCCAGT TATTCCTCCT
    CCTGTGGTTG CTAATGCTGG TGTCACGGAG GTCAGCCCTG AGTTCTTGGC AGCGTTGCCA
    CCAGCCATTC AGGAAGAGGT CCTAGCACAG CAGCGAGCAG AACAGCAGAG GCGGGAACTT
    GCTCAGACCA CTACGTCTGA TACACCCATG GATCCTGTGA CTTTCATTCA GACGTTACCT
    TCTGATCTAC GTCGTAGTGT GTTAGAAGAC ATGGAGGACA GTGTTTTAGC TGTGATGCCA
    CCTGACATTG CTGCAGAGGC ACAGGCTCTA AGGCGAGAGC AAGAAGCTCG GCAGAGGCAA
    CTAATGCATG AACGACTGTT CGGGCACAGT AGCACATCTG CACTCTCTGC CATCCTTAGG
    AGTCCTGCTT TCACGAGTCG CTTGAGTGGA AACCGAGGTG TTCAGTACAC ACGGTTGGCT
    GTACAGAGAG GGAACACTTT CCAAATGGGA AGCAGCAGTA CACATACCAG ACCAACCGGT
    AGTAATGTTG ACAGCCTACT ACGATTACGA GGCCGACTAT TACTTGATCA TGAAGCCCTC
    TCCTGTCTGC TTGTCCTACT GTTTGTTGAT GAACCCAAGC TAAACACCAG TCGCTTGCAC
    CGTGTTCTAC GCAACCTCTG CTACCATGCA CAAACAAGGA ACTGGGTGAT TAGAAGCTTG
    TTGTCTATAC TGCAGCGTAG CAGTGAAAGT GAGCTTTGTA TAGAAAGCCC AAAGACTTCT
    ATTGGGGGCA CTGATAAACG GGGTGCAAAA ACAAGTCATG ACAACCGCCC TTTAGAATTT
    CTGCATAAGA TGGAATCGAA GAATTCCAAC CAGCTCTCCT GGCTTTCTGT ATCCATGGAC
    GCTGCTCTTG GCTGCCGTAC GAACATCTTC CAAATCCAGA GGTCCCTAGG ACGCAAGCAT
    ACAGAAAAGC ATTCAGCCTG TGGTTCTACT GTCCACATCC ATCCCCAAGC AGCCCCTGTA
    GTGTGTAGAC ATGTGCTTGA CACACTAATA CAGCTAGCTA AGGTGTTTCC CAGTCATTTC
    ACACAGCAGC GAACCAAAGA ATGTAGTGTG GAAAACAGTG ACAGCAGGGA GCGGAGTATA
    AAACTTACTT GTAGTCCGTG TCTTGCACCA TCTAGTAGTA TTGGAGCAAG CAACATCTCC
    ACAGATTTCT GGGACTTGCT AGTCAAGCTA GACAACATGA ATGTGAGCCG GAAGGGGAAA
    ACGTCTATAA AATCTGTGCC AGTGAGCTCT GGTTCTGAAG GCGAGAGCAA TCAGTGCAGC
    CTGGAGGCCT CCCCCTTAGG ACAGCTTATG AATATGCTGT CTCATTCTGT TATTCGCCGC
    AGTTCGCTGC TTACTGAAAA GCTGCTGAGA CTTCTCTCCC TTATCTCTAT TGCCCTTCCT
    GATAATAAGG CTTCTGAAAC AGCAGGCAAT CAGAGCAATT CTATTACCCC AGCACCTGCA
    CCTACGCCTG CAACTGCACC AGCCCCTGTG CCTGTACCTG CTGTAACAGT TCCAGTTGTT
    GTGAGCACAA ACATAGTGAC AACTGTTACA ACCAAAAGTG GAAAATCTCC TGCAAAATCA
    AGTGAAGTCA GCAATGCACC AGATAAGATG GCAGCTTCTG GCCTTACAGA GAAACAGCTG
    CAGTTGTCTG TTGAGGTTCT GACATCACAC TCGTGTTCAG AAGAAGGACT GGAGGATGCC
    GCCAATGTTC TACTTCAGCT TTCCAGAGGC GACCCCAGTA CACGGGACAC TGTACTGAAA
    CTTCTGCTTA GCGGAGCACA TCACTTGGGG TTTACTCTCT GCAAACAGAT AGGTACACTA
    CTAGCAGAGC TCCGGGAATA TAACCTAGAG CAGCAGCGTC GTGCACAGTG TGAAGTTCTT
    TCTCCAGATG GTATGCCTGA GGAGCAACCC CACAGCACAA AGCCAAAAGG CAAAATGCAG
    AGCAGGTCTC TCCCCACCCC ACCTATGCGA TCCAGGTTCG ACATTGCAGA GAATGTGGTG
    ATTGTGGCTT CCCAAAAGCG TCCACTGGGA GGAAGAGAGC TGCAGCTCCC CTCAATGTCT
    ATGCTGACGT CAAAGACTTC CACACAAAAA TTCTTTCTTC GAGTGCTGCA GGTTATCATC
    CAGCTTCGCG ATGACACTCG CAGAGCCAAC AAGAAGGCCA AGCAGACCAG CAGATTAGGC
    ACTGCCAGTC TGGCCTCGGC TAGCAGCATC CAAGCAGCTG TGCAGCAGCT GGAGGCTGAG
    GCTGATGCCA TTATACAAAT GGTACGTGAG GGTCAAAGGG CGCGGAGACA GCTGCAAACT
    GCAACGGCGG AGGGTTCTGC TCGCAGGGAG GAGCAGCCTA TGGATGTTGA CCAGCCTTCG
    CCTGCTACAC AGGAGCCTCA GTCAGTTGCT TCTGAAGGTT CTCAGGCTGC TGAAAAAAAA
    GAAGAGGAGA GGTTACCAGA GTTACCGCTG TTAAGTGAGC AGCTAAGTTT AGATGAGCTG
    TGGGACATGT TGGGAGAGTG CCTGAAGGAA CTTGAGGAAT CTCATGACCA GCATGCCGTA
    TTAGTGTTAC AGCCAGCCGT AGAAGCCTTT TTCCTTGTTC ATGCAACAGA GCGGGAGAGC
    AAGCCTCCTG TGCGGGACAC TCGGGAAAGC CAGTTATCTC ACATTAAGGA TGAACCACCT
    CCCCTGTCTC CTGCACCCCT CACTCCAGCC ACACCTTCTT CCTTGGATCC TTTCTTCTCT
    CGTGAACCTT CATCTATGCA CATTTCCTCT AGTTTGCCTC CTGACACTCA GAAGTTCCTA
    AGATTTGCTG AGACTCATCG GACTGTATTA AATCAGATCC TTCGCCAGTC CACCACACAC
    CTAGCTGATG GGCCCTTTGC TGTGCTTGTA GACTATATCA GAGTCCTTGA CTTTGATGTT
    AAGCGCAAAT ATTTCCGACA GGAGTTGGAG CGACTGGATG AAGGGCTAAG AAAAGAGGAT
    ATGGCAGTGC ATGTGCGGCG AGATCATGTG TTTGAGGATT CCTACCGGGA GCTGCACCGC
    AAATCCCCTG AAGAAATGAA GAATAGATTG TACATAGTAT TTGAAGGAGA AGAAGGGCAG
    GATGCAGGTG GGCTGCTTCG GGAGTGGTAC ATGATTATCT CCAGGGAGAT GTTCAATCCC
    ATGTATGCTT TGTTCCGCAC TTCCCCAGGT GACCGCGTCA CCTACACTAT TAATCCTTCC
    TCCCACTGTA ACCCTAACCA CCTCAGCTAC TTCAAGTTTG TGGGCCGGAT AGTTGCCAAA
    GCGGTTTATG ACAACCGGCT ACTGGAGTGT TACTTCACTA GGTCCTTCTA CAAGCATATA
    TTGGGCAAGT CTGTCAGATA CACAGACATG GAGAGTGAAG ATTATCACTT CTATCAGGGT
    CTGGTGTACT TGCTTGAAAA TGATGTCTCC ACACTGGGAT ATGACTTGAC TTTCAGCACT
    GAGGTTCAAG AATTTGGTGT CTGTGAAGTC CGAGACCTAA AACCAAACGG TGCAAACATT
    CTGGTAACAG AAGAAAACAA AAAGGAGTAT GTTCATCTTG TGTGCCAGAT GAAGATGACA
    GGTGCAATAC GTAAGCAATT GGCTGCCTTC CTTGAGGGAT TTTATGAGAT AATCCCCAAA
    CGTCTGATCT CCATCTTCAC TGAGCAGGAA TTGGAACTGC TTATCTCTGG CCTTCCCACC
    ATAGATATTG ATGACCTAAA ATCAAACACT GAGTATCACA AATACCAGTC TAACTCCATC
    CAGATTCAGT GGTTCTGGAG AGCGTTGCGC TCATTCGATC AAGCTGACCG GGCAAAGTTC
    CTTCAGTTTG TTACTGGAAC TTCAAAGGTC CCATTACAGG GTTTTGCTGC GCTGGAAGGA
    ATGAACGGCA TCCAGAAATT CCAAATCCAC AGAGATGATA GATCTACAGA CAGGCTGCCT
    TCTGCGCACA CGTGCTTCAA CCAGTTGGAC CTTCCAGCCT ATGAGAGTTA CGAGAAGCTA
    CGTCACATGC TGCTGTTAGC CATCCAGGAG TGCTCTGAGG GCTTTGGCCT TGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65591
    Clone ID Related Accession (Same CDS sequence) XM_031892171.1
    Accession Version XM_031892171.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1321
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    1441
    1501
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    1981
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    12241
    12301
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    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGGTGCTG ATGGATATGG GTTTCACAAG GGAGCATGCT
    CTGGAAGCTC TGCTTAACAC GAGTACTATG GAGCAGGCCA CTGAATATCT CCTCACCCAC
    CCTCCACCTC TTATTGGCGG AGTTGTACGG GACTTAAGTA TGTCAGAGGA AGATCAGATG
    CTGAGAGCCA TTGCCATGTC GCTGGGACAA GATATCCCTA TGGAACAGAG GGCAGAACTG
    CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG GAGGAACGCA AGGCACGAGA AAAACAAGAG
    GAAGAGGAAG CTAAATGCTT GGAAAAGTTT CAAGATGCAG AACCCCTGGA ACAGGATGAG
    CTTCAAGGAT TCACAGACAC CATGCTCCCT GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA
    GACACAGTCT ATAGAGTGTG TGATCTTATA ATGACTGCCA TAAAGCGCAA TGGGGCAGAC
    TATCGAGACA TGATTCTAAA ACAAGTGGTT AACCAGGTAT GGGAAGCCGC TGATGTTCTT
    ATTAAAGCTG CACTGCCGCT GACCACAAGT GACACAAAAA CTGTGTCTGA GTGGATCAGC
    CAAATGGCTA CTCTTCCTCA AGCTTCTAAC TTGGCCACTC GCATCCTTTT GCTCACACTG
    CTGTTTGAGG AGCTAAAACT GCCATGTGCT AGAGTTGTTG AAACAAGTGG AATACTTAAC
    GTACTAATTA AGCTGCTTGA AGTGGTTCAG CCATGTTTGC AGGCAGCCAA GGAGCAAAAG
    GAAGTTCAGA CACCAAAGTG GATTACACCA GTCCTCCTCT TAATAGATTT TTATGAGAAG
    ACCTCAATTT CTTCTAAAAG AAGAATGCAA ATGAACAGGT ACCTTCAAGC TAATGGAAAC
    AACTGGCGAT GGTTTGATGA TCGGTCTGGC CGTTGGTGCA GCTACAGTGC TAGTAACAAC
    AGCACTATTG ATGCAGCCTG GAAAGCAGGA GAAACATGTG TGCGATTTAC AGCTGGCCGT
    CGAAGATATA CTGTGCAGTT TACCACAATG GTTCAGGTAA ATGAAGAGAC CGGCAATAGA
    CGACCTGTCA TGCTGACTCT CTTGCGTGTT CCGAGACTTT TAAAGAGTGG AAAAAATGGA
    AATGGACAGG AACTTGAAAA GACACTTGAG GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA
    GACGAAAAAG TGCCTGAGCC CATTCCAGTT CCTGAAACCT CTTCCACTGA GAAAGATGCA
    CCAACTGAAG AGCCCAAAGT TGGAGACATA GTAATTCAGG GTCTGGCTGA AGAAATGGTG
    ACCGTCCTTA TACGTTCCTG CGTCGGCATG CTTGGTGTTC CTGTTGATCC AGATACTCTT
    CATGCAACCT TGCGTCTTTG CCTGCGTCTC ACACGTGACC ACAAATATGC CATGATGTTT
    GCAGAGCTTA AGAGCACTCG AATGATCCTC AGCTTAACTC AAAGCTCTGG ATTCAATGGC
    TTTACTCCCC TTGTGACGCT CCTATTTAGG CACATCATAG AGGATCCTTG CACCCTCCAG
    CACACCATGG AAAAGGTTGT ACGGTCTGCA GCAACTAGTG GGGCAGGGAG TACCACATCT
    GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT GAAATCAACT ATATACTTAG AGTACTGGGT
    CCAGCAGCTT GTCGAAACCC TGACATTTTC ACTGAAGTGG CAAACAGTTG CATTCGCATT
    GCTCTCCCTG CACCTCGTGG ATCTGGAACT GCCTCTGATG ATGAGTTTGA GAACCTTCGG
    ATCAAAGGTC CTAATGCAGT TCAGTTGGTT AAAACCACCC CTATAAAGCC CTCCACCTTG
    CCAGTCATCC CTGATACAAT TAAGGAAGTA ATCTATGACA TGCTGAATGC ACTGGCAGCT
    TACCACGCCC CTGAGGAAGT ATCTGAAAAG TCTGAGCAAA AACCTGTTGG CATGAACCAG
    GAAGTCAGCC AGATCCTACA GGACATGGGT GATGAGGTTT TCCAGCAATA TCGGGACTTA
    ACCAGACAGG GAAGCGACTT TGATGGTCAG ACCAGTTTTG CCATCAACGC GCGAGTCTTT
    ACTGCTGACA GCACAGCTGC CGAAACACCT CAGTCTGGTA CACCTCAAGG TGAAACTGCC
    TTAACACCTG AAGAAAACCG AGAAGGGTTA AAGAAAGATA AGGAAGGAGA CCGTAGTTCA
    GAAGATGGAA AGCAGAAGGC AAAAGGTAGT AAGCCTTTGC TGCCTACCTC TTCCATTCTT
    CGACTACTTG CTGAACTTGT CAGGTCCTAT GTCGGAATAG CCTCGCTGAT CGCCACCTAC
    AATTACACTG CAGGACAATC TGAACTAATT AAAGAGGATT GCAGTGTATT GGCGTTTGTG
    CTGGATCACC TGCTACCCCA CACACATAAT GCAGAGGACA AGGACACACC AGCCCTAGCC
    CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA GGAAGTGGCA CTGATGCCCA GGTTGCACTA
    GTGAATGAAG TAAAGGCAGC TCTTGGCAGA GCCCTAGCAA TGGCTGAGAG CACGGAGAAG
    CATGCTAGGT TGCAGGCTGT GATGTGCATT ATCAGTACCA TTATGGAGTC ATGTCCATCC
    ACCTCCAGCT TCTACAGCAC AGCAACCACC AAAACCCAGC ACAATGGCAT GAACAACATC
    ATCAGACTCT TTTTGAAAAA GGGGCTGGTT AGTGATTTAG CCAGGGTCCC TCACAGCCTT
    GATTTATCCA GTCCAAATAT GGCAAACACC GTGAATGCAG CACTGAAGCC ACTCGAGACA
    CTGTCTCGCA TTGTCAATCA ACCCAGCAGT TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG
    AATAAATCTG AGGCAGATAC ACATGGAACA GCCCAGGATC CCAACAGTAA TCAGCCAGAT
    ACAGGTGAAA CAGGAACAGA AGTGCAGGAA GAGGACCCAG ATGCTTCTCA TGCTGAAGTA
    GCTGATGGAG ACATCATGGA TGGAGAGACA GAGACTGATG CTGTGGTTAT TGCTGGCCAG
    CCTGAAGTCC TTACTACACA AGAAATGCAG GTAGAGAATG AGCTTGAAGA TCTGATAGAT
    GAACTTCTTG AAAGGGATGG TGCTGCAGGT AACCGGGTCG GAGAAGATGA ATCACAGGAA
    GATGTGCTGA TGGATGAGCC TCCTACTAAC CTCAGCCAAG CCTCCACTCT TCAGGCTAAT
    CGAGAAGACC CAGAGGATGA GGAAGAGCAC ACCCAAGAAG AGGACAGCAG TGGCAGCAAT
    GAAGATGAAG ATGACAGCCA GGAAGAGGAG GAGGAAGAGG AAGATGAGGA CGATCAGGAT
    GATGAGGAGG GTGAGGAAGG GGATGAGGAT GATGATGATG ACTGTTCAGA GATGGAACTG
    GATGAGGATT ATCCAGACAT GAATGCCTCA CCTCTTGTAC GCTTTGAACG TTTTGACAGA
    GAAGATGATT TAATCATAGA GTTTGATAAC ATGTTCTCCA GTGCCACTGA GATCCCTCCA
    TCCCCTGGTA ACATCCCTAC TACACACCCC CTAATGGTGC GACACGCTGA TCACGGCTCC
    TTGACTCTTG GCAGTGGCAC TTCCACTACT CGCCTGGCAC AGGGCATCGG GCGCAGCCAG
    AGAACACTCC GACAACTCAC AGCCAACACC GGTCACACAA TACATGTTCA TTATCCTGGC
    AACAGGCAGC CAAATCCACC TTTAATCCTC CAGAGGTTGC TGGGGCCCTC AGCCGCTGCT
    GACATCTTGC AACTAAGCAG CAGTCTTCCT CTGCAGAGTC GTGGCCGAGC ACGTCTACTT
    GTTGGCAACG ATGATGTCCA CATCATAGCA CGCTCTGATG ATGAGATGCT TGATGATTTT
    TTCCATGATC AGAGTACAGC CACAAGTCAG GCTGGCACAC TTTCCAGCAT TCCAACTGCG
    CTGACCCGCT GGACAGAAGA GTGTAAGGTC TTGGATGCTG AAAGCATGCA TGACTGTGTA
    TCTGTGGTCA AAGTCCCAAT CATCAATCAT TTGGAATCTT TGCGTGATGA GGAGCTTGAG
    GAGCGCAGGG AAAAAAGAAG GAAACAACTA GCAGAAGAGG AGGCCAAGAT GGCAGAGAAA
    TCCAAGGAGG AGAAGGAAGC AAAGGAGCAA AATTCACAGG GTGCCAATTC AAAGCAGAAT
    ACCCCATTTG AACTGAGCCT TGCAGATCAA GATGGAAGTG GTCGTCCTGA AAATTTCCCA
    ACTGCACCAT CTTCTGCAGA AGGAACTCCA ACAAGTCCTG AAATTGTTCG TTTAGATCCC
    ACCCTAAGGG CTACTGAGGC TACTCTGGCA GAGCTGACTC AGGGTCTGCC TGCTGATACA
    GGCACGGAGG ATGTTGGGCA ACAACTCATG CCATCTCACC AAGAGGACTC TGGACCAGGA
    CGGCCAAGTG TAGAGGGGGA TGCCACACAA ATGGAGCTAT CGCCAGCACC CACTATAACC
    TCCTTATCAC CAGAGCGAGC AGAGGATTCA GATGCTTTGA CAGCTGTTAG TAGCCAACTA
    GAGGGCTCGC CTATGGACAC CAGCAGTCTT GCCTCTGGGC CCTTAGAGGA TGGCGTAGGG
    GAGGCACCTG GAATTGTTAA TCCAGAGCAG CCTCTTGTGG TTGGCAGTAG CATTGCTGTT
    GGACAGCAAC CAGTGGAACC ACCGACGACC TGTGAAACCC AACCAGTAGT TTCTCAACAC
    AGTGATAGCT CACAAATTGC CCCTTCCTCT GAGTTGCCTT CCACAAGAGA AAGTGCTGTG
    ACCTTGTTGG CTACAGATTC TAGTGGTATC CTTGAAGAAC CTTTGCCTTC TACAAGCAGT
    GAAGAGGAAG ACCCATTAGC AGGCATAAAT CTTCCAGAGG GTGTGGATCC TTCTTTCCTA
    GCGGCTCTTC CTGAAGACAT CCGCAGAGAG GTGCTGCAGA ATCAGTTGGG CATACGGCCG
    CCATCGCGAC CTCCACCCAC TGCTGCCCCA GTTATTCCTC CTCCTGTGGT TGCTAATGCT
    GGTGTCACGG AGGTCAGCCC TGAGTTCTTG GCAGCGTTGC CACCAGCCAT TCAGGAAGAG
    GTCCTAGCAC AGCAGCGAGC AGAACAGCAG AGGCGGGAAC TTGCTCAGAC CACTACGTCT
    GATACACCCA TGGATCCTGT GACTTTCATT CAGACGTTAC CTTCTGATCT ACGTCGTAGT
    GTGTTAGAAG ACATGGAGGA CAGTGTTTTA GCTGTGATGC CACCTGACAT TGCTGCAGAG
    GCACAGGCTC TAAGGCGAGA GCAAGAAGCT CGGCAGAGGC AACTAATGCA TGAACGACTG
    TTCGGGCACA GTAGCACATC TGCACTCTCT GCCATCCTTA GGAGTCCTGC TTTCACGAGT
    CGCTTGAGTG GAAACCGAGG TGTTCAGTAC ACACGGTTGG CTGTACAGAG AGGGAACACT
    TTCCAAATGG GAAGCAGCAG TACACATACC AGACCAACCG GTAGTAATGT TGACAGCCTA
    CTACGATTAC GAGGCCGACT ATTACTTGAT CATGAAGCCC TCTCCTGTCT GCTTGTCCTA
    CTGTTTGTTG ATGAACCCAA GCTAAACACC AGTCGCTTGC ACCGTGTTCT ACGCAACCTC
    TGCTACCATG CACAAACAAG GAACTGGGTG ATTAGAAGCT TGTTGTCTAT ACTGCAGCGT
    AGCAGTGAAA GTGAGCTTTG TATAGAAAGC CCAAAGACTT CTATTGGGGG CACTGATAAA
    CGGGGTGCAA AAACAAGTCA TGACAACCGC CCTTTAGAAT TTCTGCATAA GATGGAATCG
    AAGAATTCCA ACCAGCTCTC CTGGCTTTCT GTATCCATGG ACGCTGCTCT TGGCTGCCGT
    ACGAACATCT TCCAAATCCA GAGGTCCCTA GGACGCAAGC ATACAGAAAA GCATTCAGCC
    TGTGGTTCTA CTGTCCACAT CCATCCCCAA GCAGCCCCTG TAGTGTGTAG ACATGTGCTT
    GACACACTAA TACAGCTAGC TAAGGTGTTT CCCAGTCATT TCACACAGCA GCGAACCAAA
    GAATGTAGTG TGGAAAACAG TGACAGCAGG GAGCGGAGTA TAAAACTTAC TTGTAGTCCG
    TGTCTTGCAC CATCTAGTAG TATTGGAGCA AGCAACATCT CCACAGATTT CTGGGACTTG
    CTAGTCAAGC TAGACAACAT GAATGTGAGC CGGAAGGGGA AAACGTCTAT AAAATCTGTG
    CCAGTGAGCT CTGGTTCTGA AGGCGAGAGC AATCAGTGCA GCCTGGAGGC CTCCCCCTTA
    GGACAGCTTA TGAATATGCT GTCTCATTCT GTTATTCGCC GCAGTTCGCT GCTTACTGAA
    AAGCTGCTGA GACTTCTCTC CCTTATCTCT ATTGCCCTTC CTGATAATAA GGCTTCTGAA
    ACAGCAGGCA ATCAGAGCAA TTCTATTACC CCAGCACCTG CACCTACGCC TGCAACTGCA
    CCAGCCCCTG TGCCTGTACC TGCTGTAACA GTTCCAGTTG TTGTGAGCAC AAACATAGTG
    ACAACTGTTA CAACCAAAAG TGGAAAATCT CCTGCAAAAT CAAGTGAAGT CAGCAATGCA
    CCAGATAAGA TGGCAGCTTC TGGCCTTACA GAGAAACAGC TGCAGTTGTC TGTTGAGGTT
    CTGACATCAC ACTCGTGTTC AGAAGAAGGA CTGGAGGATG CCGCCAATGT TCTACTTCAG
    CTTTCCAGAG GCGACCCCAG TACACGGGAC ACTGTACTGA AACTTCTGCT TAGCGGAGCA
    CATCACTTGG GGTTTACTCT CTGCAAACAG ATAGGTACAC TACTAGCAGA GCTCCGGGAA
    TATAACCTAG AGCAGCAGCG TCGTGCACAG TGTGAAGTTC TTTCTCCAGA TGGTATGCCT
    GAGGAGCAAC CCCACAGCAC AAAGCCAAAA GGCAAAATGC AGAGCAGTGT TAGGTCTCTC
    CCCACCCCAC CTATGCGATC CAGGTTCGAC ATTGCAGAGA ATGTGGTGAT TGTGGCTTCC
    CAAAAGCGTC CACTGGGAGG AAGAGAGCTG CAGCTCCCCT CAATGTCTAT GCTGACGTCA
    AAGACTTCCA CACAAAAATT CTTTCTTCGA GTGCTGCAGG TTATCATCCA GCTTCGCGAT
    GACACTCGCA GAGCCAACAA GAAGGCCAAG CAGACCAGCA GATTAGGCAC TGCCAGTCTG
    GCCTCGGCTA GCAGCATCCA AGCAGCTGTG CAGCAGCTGG AGGCTGAGGC TGATGCCATT
    ATACAAATGG TACGTGAGGG TCAAAGGGCG CGGAGACAGC TGCAAACTGC AACGGCGGAG
    GGTTCTGCTC GCAGGGAGGA GCAGCCTATG GATGTTGACC AGCCTTCGCC TGCTACACAG
    GAGCCTCAGT CAGTTGCTTC TGAAGGTTCT CAGGCTGCTG AAAAAAAAGA AGAGGAGAGG
    TTACCAGAGT TACCGCTGTT AAGTGAGCAG CTAAGTTTAG ATGAGCTGTG GGACATGTTG
    GGAGAGTGCC TGAAGGAACT TGAGGAATCT CATGACCAGC ATGCCGTATT AGTGTTACAG
    CCAGCCGTAG AAGCCTTTTT CCTTGTTCAT GCAACAGAGC GGGAGAGCAA GCCTCCTGTG
    CGGGACACTC GGGAAAGCCA GTTATCTCAC ATTAAGGATG AACCACCTCC CCTGTCTCCT
    GCACCCCTCA CTCCAGCCAC ACCTTCTTCC TTGGATCCTT TCTTCTCTCG TGAACCTTCA
    TCTATGCACA TTTCCTCTAG TTTGCCTCCT GACACTCAGA AGTTCCTAAG ATTTGCTGAG
    ACTCATCGGA CTGTATTAAA TCAGATCCTT CGCCAGTCCA CCACACACCT AGCTGATGGG
    CCCTTTGCTG TGCTTGTAGA CTATATCAGA GTCCTTGACT TTGATGTTAA GCGCAAATAT
    TTCCGACAGG AGTTGGAGCG ACTGGATGAA GGGCTAAGAA AAGAGGATAT GGCAGTGCAT
    GTGCGGCGAG ATCATGTGTT TGAGGATTCC TACCGGGAGC TGCACCGCAA ATCCCCTGAA
    GAAATGAAGA ATAGATTGTA CATAGTATTT GAAGGAGAAG AAGGGCAGGA TGCAGGTGGG
    CTGCTTCGGG AGTGGTACAT GATTATCTCC AGGGAGATGT TCAATCCCAT GTATGCTTTG
    TTCCGCACTT CCCCAGGTGA CCGCGTCACC TACACTATTA ATCCTTCCTC CCACTGTAAC
    CCTAACCACC TCAGCTACTT CAAGTTTGTG GGCCGGATAG TTGCCAAAGC GGTTTATGAC
    AACCGGCTAC TGGAGTGTTA CTTCACTAGG TCCTTCTACA AGCATATATT GGGCAAGTCT
    GTCAGATACA CAGACATGGA GAGTGAAGAT TATCACTTCT ATCAGGGTCT GGTGTACTTG
    CTTGAAAATG ATGTCTCCAC ACTGGGATAT GACTTGACTT TCAGCACTGA GGTTCAAGAA
    TTTGGTGTCT GTGAAGTCCG AGACCTAAAA CCAAACGGTG CAAACATTCT GGTAACAGAA
    GAAAACAAAA AGGAGTATGT TCATCTTGTG TGCCAGATGA AGATGACAGG TGCAATACGT
    AAGCAATTGG CTGCCTTCCT TGAGGGATTT TATGAGATAA TCCCCAAACG TCTGATCTCC
    ATCTTCACTG AGCAGGAATT GGAACTGCTT ATCTCTGGCC TTCCCACCAT AGATATTGAT
    GACCTAAAAT CAAACACTGA GTATCACAAA TACCAGTCTA ACTCCATCCA GATTCAGTGG
    TTCTGGAGAG CGTTGCGCTC ATTCGATCAA GCTGACCGGG CAAAGTTCCT TCAGTTTGTT
    ACTGGAACTT CAAAGGTCCC ATTACAGGGT TTTGCTGCGC TGGAAGGAAT GAACGGCATC
    CAGAAATTCC AAATCCACAG AGATGATAGA TCTACAGACA GGCTGCCTTC TGCGCACACG
    TGCTTCAACC AGTTGGACCT TCCAGCCTAT GAGAGTTACG AGAAGCTACG TCACATGCTG
    CTGTTAGCCA TCCAGGAGTG CTCTGAGGGC TTTGGCCTTG CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748031.1
    CDS671..13795
    Translation

    Target ORF information:

    RefSeq Version XM_031892171.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892171.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGGTGCTG ATGGATATGG GTTTCACAAG GGAGCATGCT
    CTGGAAGCTC TGCTTAACAC GAGTACTATG GAGCAGGCCA CTGAATATCT CCTCACCCAC
    CCTCCACCTC TTATTGGCGG AGTTGTACGG GACTTAAGTA TGTCAGAGGA AGATCAGATG
    CTGAGAGCCA TTGCCATGTC GCTGGGACAA GATATCCCTA TGGAACAGAG GGCAGAACTG
    CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG GAGGAACGCA AGGCACGAGA AAAACAAGAG
    GAAGAGGAAG CTAAATGCTT GGAAAAGTTT CAAGATGCAG AACCCCTGGA ACAGGATGAG
    CTTCAAGGAT TCACAGACAC CATGCTCCCT GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA
    GACACAGTCT ATAGAGTGTG TGATCTTATA ATGACTGCCA TAAAGCGCAA TGGGGCAGAC
    TATCGAGACA TGATTCTAAA ACAAGTGGTT AACCAGGTAT GGGAAGCCGC TGATGTTCTT
    ATTAAAGCTG CACTGCCGCT GACCACAAGT GACACAAAAA CTGTGTCTGA GTGGATCAGC
    CAAATGGCTA CTCTTCCTCA AGCTTCTAAC TTGGCCACTC GCATCCTTTT GCTCACACTG
    CTGTTTGAGG AGCTAAAACT GCCATGTGCT AGAGTTGTTG AAACAAGTGG AATACTTAAC
    GTACTAATTA AGCTGCTTGA AGTGGTTCAG CCATGTTTGC AGGCAGCCAA GGAGCAAAAG
    GAAGTTCAGA CACCAAAGTG GATTACACCA GTCCTCCTCT TAATAGATTT TTATGAGAAG
    ACCTCAATTT CTTCTAAAAG AAGAATGCAA ATGAACAGGT ACCTTCAAGC TAATGGAAAC
    AACTGGCGAT GGTTTGATGA TCGGTCTGGC CGTTGGTGCA GCTACAGTGC TAGTAACAAC
    AGCACTATTG ATGCAGCCTG GAAAGCAGGA GAAACATGTG TGCGATTTAC AGCTGGCCGT
    CGAAGATATA CTGTGCAGTT TACCACAATG GTTCAGGTAA ATGAAGAGAC CGGCAATAGA
    CGACCTGTCA TGCTGACTCT CTTGCGTGTT CCGAGACTTT TAAAGAGTGG AAAAAATGGA
    AATGGACAGG AACTTGAAAA GACACTTGAG GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA
    GACGAAAAAG TGCCTGAGCC CATTCCAGTT CCTGAAACCT CTTCCACTGA GAAAGATGCA
    CCAACTGAAG AGCCCAAAGT TGGAGACATA GTAATTCAGG GTCTGGCTGA AGAAATGGTG
    ACCGTCCTTA TACGTTCCTG CGTCGGCATG CTTGGTGTTC CTGTTGATCC AGATACTCTT
    CATGCAACCT TGCGTCTTTG CCTGCGTCTC ACACGTGACC ACAAATATGC CATGATGTTT
    GCAGAGCTTA AGAGCACTCG AATGATCCTC AGCTTAACTC AAAGCTCTGG ATTCAATGGC
    TTTACTCCCC TTGTGACGCT CCTATTTAGG CACATCATAG AGGATCCTTG CACCCTCCAG
    CACACCATGG AAAAGGTTGT ACGGTCTGCA GCAACTAGTG GGGCAGGGAG TACCACATCT
    GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT GAAATCAACT ATATACTTAG AGTACTGGGT
    CCAGCAGCTT GTCGAAACCC TGACATTTTC ACTGAAGTGG CAAACAGTTG CATTCGCATT
    GCTCTCCCTG CACCTCGTGG ATCTGGAACT GCCTCTGATG ATGAGTTTGA GAACCTTCGG
    ATCAAAGGTC CTAATGCAGT TCAGTTGGTT AAAACCACCC CTATAAAGCC CTCCACCTTG
    CCAGTCATCC CTGATACAAT TAAGGAAGTA ATCTATGACA TGCTGAATGC ACTGGCAGCT
    TACCACGCCC CTGAGGAAGT ATCTGAAAAG TCTGAGCAAA AACCTGTTGG CATGAACCAG
    GAAGTCAGCC AGATCCTACA GGACATGGGT GATGAGGTTT TCCAGCAATA TCGGGACTTA
    ACCAGACAGG GAAGCGACTT TGATGGTCAG ACCAGTTTTG CCATCAACGC GCGAGTCTTT
    ACTGCTGACA GCACAGCTGC CGAAACACCT CAGTCTGGTA CACCTCAAGG TGAAACTGCC
    TTAACACCTG AAGAAAACCG AGAAGGGTTA AAGAAAGATA AGGAAGGAGA CCGTAGTTCA
    GAAGATGGAA AGCAGAAGGC AAAAGGTAGT AAGCCTTTGC TGCCTACCTC TTCCATTCTT
    CGACTACTTG CTGAACTTGT CAGGTCCTAT GTCGGAATAG CCTCGCTGAT CGCCACCTAC
    AATTACACTG CAGGACAATC TGAACTAATT AAAGAGGATT GCAGTGTATT GGCGTTTGTG
    CTGGATCACC TGCTACCCCA CACACATAAT GCAGAGGACA AGGACACACC AGCCCTAGCC
    CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA GGAAGTGGCA CTGATGCCCA GGTTGCACTA
    GTGAATGAAG TAAAGGCAGC TCTTGGCAGA GCCCTAGCAA TGGCTGAGAG CACGGAGAAG
    CATGCTAGGT TGCAGGCTGT GATGTGCATT ATCAGTACCA TTATGGAGTC ATGTCCATCC
    ACCTCCAGCT TCTACAGCAC AGCAACCACC AAAACCCAGC ACAATGGCAT GAACAACATC
    ATCAGACTCT TTTTGAAAAA GGGGCTGGTT AGTGATTTAG CCAGGGTCCC TCACAGCCTT
    GATTTATCCA GTCCAAATAT GGCAAACACC GTGAATGCAG CACTGAAGCC ACTCGAGACA
    CTGTCTCGCA TTGTCAATCA ACCCAGCAGT TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG
    AATAAATCTG AGGCAGATAC ACATGGAACA GCCCAGGATC CCAACAGTAA TCAGCCAGAT
    ACAGGTGAAA CAGGAACAGA AGTGCAGGAA GAGGACCCAG ATGCTTCTCA TGCTGAAGTA
    GCTGATGGAG ACATCATGGA TGGAGAGACA GAGACTGATG CTGTGGTTAT TGCTGGCCAG
    CCTGAAGTCC TTACTACACA AGAAATGCAG GTAGAGAATG AGCTTGAAGA TCTGATAGAT
    GAACTTCTTG AAAGGGATGG TGCTGCAGGT AACCGGGTCG GAGAAGATGA ATCACAGGAA
    GATGTGCTGA TGGATGAGCC TCCTACTAAC CTCAGCCAAG CCTCCACTCT TCAGGCTAAT
    CGAGAAGACC CAGAGGATGA GGAAGAGCAC ACCCAAGAAG AGGACAGCAG TGGCAGCAAT
    GAAGATGAAG ATGACAGCCA GGAAGAGGAG GAGGAAGAGG AAGATGAGGA CGATCAGGAT
    GATGAGGAGG GTGAGGAAGG GGATGAGGAT GATGATGATG ACTGTTCAGA GATGGAACTG
    GATGAGGATT ATCCAGACAT GAATGCCTCA CCTCTTGTAC GCTTTGAACG TTTTGACAGA
    GAAGATGATT TAATCATAGA GTTTGATAAC ATGTTCTCCA GTGCCACTGA GATCCCTCCA
    TCCCCTGGTA ACATCCCTAC TACACACCCC CTAATGGTGC GACACGCTGA TCACGGCTCC
    TTGACTCTTG GCAGTGGCAC TTCCACTACT CGCCTGGCAC AGGGCATCGG GCGCAGCCAG
    AGAACACTCC GACAACTCAC AGCCAACACC GGTCACACAA TACATGTTCA TTATCCTGGC
    AACAGGCAGC CAAATCCACC TTTAATCCTC CAGAGGTTGC TGGGGCCCTC AGCCGCTGCT
    GACATCTTGC AACTAAGCAG CAGTCTTCCT CTGCAGAGTC GTGGCCGAGC ACGTCTACTT
    GTTGGCAACG ATGATGTCCA CATCATAGCA CGCTCTGATG ATGAGATGCT TGATGATTTT
    TTCCATGATC AGAGTACAGC CACAAGTCAG GCTGGCACAC TTTCCAGCAT TCCAACTGCG
    CTGACCCGCT GGACAGAAGA GTGTAAGGTC TTGGATGCTG AAAGCATGCA TGACTGTGTA
    TCTGTGGTCA AAGTCCCAAT CATCAATCAT TTGGAATCTT TGCGTGATGA GGAGCTTGAG
    GAGCGCAGGG AAAAAAGAAG GAAACAACTA GCAGAAGAGG AGGCCAAGAT GGCAGAGAAA
    TCCAAGGAGG AGAAGGAAGC AAAGGAGCAA AATTCACAGG GTGCCAATTC AAAGCAGAAT
    ACCCCATTTG AACTGAGCCT TGCAGATCAA GATGGAAGTG GTCGTCCTGA AAATTTCCCA
    ACTGCACCAT CTTCTGCAGA AGGAACTCCA ACAAGTCCTG AAATTGTTCG TTTAGATCCC
    ACCCTAAGGG CTACTGAGGC TACTCTGGCA GAGCTGACTC AGGGTCTGCC TGCTGATACA
    GGCACGGAGG ATGTTGGGCA ACAACTCATG CCATCTCACC AAGAGGACTC TGGACCAGGA
    CGGCCAAGTG TAGAGGGGGA TGCCACACAA ATGGAGCTAT CGCCAGCACC CACTATAACC
    TCCTTATCAC CAGAGCGAGC AGAGGATTCA GATGCTTTGA CAGCTGTTAG TAGCCAACTA
    GAGGGCTCGC CTATGGACAC CAGCAGTCTT GCCTCTGGGC CCTTAGAGGA TGGCGTAGGG
    GAGGCACCTG GAATTGTTAA TCCAGAGCAG CCTCTTGTGG TTGGCAGTAG CATTGCTGTT
    GGACAGCAAC CAGTGGAACC ACCGACGACC TGTGAAACCC AACCAGTAGT TTCTCAACAC
    AGTGATAGCT CACAAATTGC CCCTTCCTCT GAGTTGCCTT CCACAAGAGA AAGTGCTGTG
    ACCTTGTTGG CTACAGATTC TAGTGGTATC CTTGAAGAAC CTTTGCCTTC TACAAGCAGT
    GAAGAGGAAG ACCCATTAGC AGGCATAAAT CTTCCAGAGG GTGTGGATCC TTCTTTCCTA
    GCGGCTCTTC CTGAAGACAT CCGCAGAGAG GTGCTGCAGA ATCAGTTGGG CATACGGCCG
    CCATCGCGAC CTCCACCCAC TGCTGCCCCA GTTATTCCTC CTCCTGTGGT TGCTAATGCT
    GGTGTCACGG AGGTCAGCCC TGAGTTCTTG GCAGCGTTGC CACCAGCCAT TCAGGAAGAG
    GTCCTAGCAC AGCAGCGAGC AGAACAGCAG AGGCGGGAAC TTGCTCAGAC CACTACGTCT
    GATACACCCA TGGATCCTGT GACTTTCATT CAGACGTTAC CTTCTGATCT ACGTCGTAGT
    GTGTTAGAAG ACATGGAGGA CAGTGTTTTA GCTGTGATGC CACCTGACAT TGCTGCAGAG
    GCACAGGCTC TAAGGCGAGA GCAAGAAGCT CGGCAGAGGC AACTAATGCA TGAACGACTG
    TTCGGGCACA GTAGCACATC TGCACTCTCT GCCATCCTTA GGAGTCCTGC TTTCACGAGT
    CGCTTGAGTG GAAACCGAGG TGTTCAGTAC ACACGGTTGG CTGTACAGAG AGGGAACACT
    TTCCAAATGG GAAGCAGCAG TACACATACC AGACCAACCG GTAGTAATGT TGACAGCCTA
    CTACGATTAC GAGGCCGACT ATTACTTGAT CATGAAGCCC TCTCCTGTCT GCTTGTCCTA
    CTGTTTGTTG ATGAACCCAA GCTAAACACC AGTCGCTTGC ACCGTGTTCT ACGCAACCTC
    TGCTACCATG CACAAACAAG GAACTGGGTG ATTAGAAGCT TGTTGTCTAT ACTGCAGCGT
    AGCAGTGAAA GTGAGCTTTG TATAGAAAGC CCAAAGACTT CTATTGGGGG CACTGATAAA
    CGGGGTGCAA AAACAAGTCA TGACAACCGC CCTTTAGAAT TTCTGCATAA GATGGAATCG
    AAGAATTCCA ACCAGCTCTC CTGGCTTTCT GTATCCATGG ACGCTGCTCT TGGCTGCCGT
    ACGAACATCT TCCAAATCCA GAGGTCCCTA GGACGCAAGC ATACAGAAAA GCATTCAGCC
    TGTGGTTCTA CTGTCCACAT CCATCCCCAA GCAGCCCCTG TAGTGTGTAG ACATGTGCTT
    GACACACTAA TACAGCTAGC TAAGGTGTTT CCCAGTCATT TCACACAGCA GCGAACCAAA
    GAATGTAGTG TGGAAAACAG TGACAGCAGG GAGCGGAGTA TAAAACTTAC TTGTAGTCCG
    TGTCTTGCAC CATCTAGTAG TATTGGAGCA AGCAACATCT CCACAGATTT CTGGGACTTG
    CTAGTCAAGC TAGACAACAT GAATGTGAGC CGGAAGGGGA AAACGTCTAT AAAATCTGTG
    CCAGTGAGCT CTGGTTCTGA AGGCGAGAGC AATCAGTGCA GCCTGGAGGC CTCCCCCTTA
    GGACAGCTTA TGAATATGCT GTCTCATTCT GTTATTCGCC GCAGTTCGCT GCTTACTGAA
    AAGCTGCTGA GACTTCTCTC CCTTATCTCT ATTGCCCTTC CTGATAATAA GGCTTCTGAA
    ACAGCAGGCA ATCAGAGCAA TTCTATTACC CCAGCACCTG CACCTACGCC TGCAACTGCA
    CCAGCCCCTG TGCCTGTACC TGCTGTAACA GTTCCAGTTG TTGTGAGCAC AAACATAGTG
    ACAACTGTTA CAACCAAAAG TGGAAAATCT CCTGCAAAAT CAAGTGAAGT CAGCAATGCA
    CCAGATAAGA TGGCAGCTTC TGGCCTTACA GAGAAACAGC TGCAGTTGTC TGTTGAGGTT
    CTGACATCAC ACTCGTGTTC AGAAGAAGGA CTGGAGGATG CCGCCAATGT TCTACTTCAG
    CTTTCCAGAG GCGACCCCAG TACACGGGAC ACTGTACTGA AACTTCTGCT TAGCGGAGCA
    CATCACTTGG GGTTTACTCT CTGCAAACAG ATAGGTACAC TACTAGCAGA GCTCCGGGAA
    TATAACCTAG AGCAGCAGCG TCGTGCACAG TGTGAAGTTC TTTCTCCAGA TGGTATGCCT
    GAGGAGCAAC CCCACAGCAC AAAGCCAAAA GGCAAAATGC AGAGCAGTGT TAGGTCTCTC
    CCCACCCCAC CTATGCGATC CAGGTTCGAC ATTGCAGAGA ATGTGGTGAT TGTGGCTTCC
    CAAAAGCGTC CACTGGGAGG AAGAGAGCTG CAGCTCCCCT CAATGTCTAT GCTGACGTCA
    AAGACTTCCA CACAAAAATT CTTTCTTCGA GTGCTGCAGG TTATCATCCA GCTTCGCGAT
    GACACTCGCA GAGCCAACAA GAAGGCCAAG CAGACCAGCA GATTAGGCAC TGCCAGTCTG
    GCCTCGGCTA GCAGCATCCA AGCAGCTGTG CAGCAGCTGG AGGCTGAGGC TGATGCCATT
    ATACAAATGG TACGTGAGGG TCAAAGGGCG CGGAGACAGC TGCAAACTGC AACGGCGGAG
    GGTTCTGCTC GCAGGGAGGA GCAGCCTATG GATGTTGACC AGCCTTCGCC TGCTACACAG
    GAGCCTCAGT CAGTTGCTTC TGAAGGTTCT CAGGCTGCTG AAAAAAAAGA AGAGGAGAGG
    TTACCAGAGT TACCGCTGTT AAGTGAGCAG CTAAGTTTAG ATGAGCTGTG GGACATGTTG
    GGAGAGTGCC TGAAGGAACT TGAGGAATCT CATGACCAGC ATGCCGTATT AGTGTTACAG
    CCAGCCGTAG AAGCCTTTTT CCTTGTTCAT GCAACAGAGC GGGAGAGCAA GCCTCCTGTG
    CGGGACACTC GGGAAAGCCA GTTATCTCAC ATTAAGGATG AACCACCTCC CCTGTCTCCT
    GCACCCCTCA CTCCAGCCAC ACCTTCTTCC TTGGATCCTT TCTTCTCTCG TGAACCTTCA
    TCTATGCACA TTTCCTCTAG TTTGCCTCCT GACACTCAGA AGTTCCTAAG ATTTGCTGAG
    ACTCATCGGA CTGTATTAAA TCAGATCCTT CGCCAGTCCA CCACACACCT AGCTGATGGG
    CCCTTTGCTG TGCTTGTAGA CTATATCAGA GTCCTTGACT TTGATGTTAA GCGCAAATAT
    TTCCGACAGG AGTTGGAGCG ACTGGATGAA GGGCTAAGAA AAGAGGATAT GGCAGTGCAT
    GTGCGGCGAG ATCATGTGTT TGAGGATTCC TACCGGGAGC TGCACCGCAA ATCCCCTGAA
    GAAATGAAGA ATAGATTGTA CATAGTATTT GAAGGAGAAG AAGGGCAGGA TGCAGGTGGG
    CTGCTTCGGG AGTGGTACAT GATTATCTCC AGGGAGATGT TCAATCCCAT GTATGCTTTG
    TTCCGCACTT CCCCAGGTGA CCGCGTCACC TACACTATTA ATCCTTCCTC CCACTGTAAC
    CCTAACCACC TCAGCTACTT CAAGTTTGTG GGCCGGATAG TTGCCAAAGC GGTTTATGAC
    AACCGGCTAC TGGAGTGTTA CTTCACTAGG TCCTTCTACA AGCATATATT GGGCAAGTCT
    GTCAGATACA CAGACATGGA GAGTGAAGAT TATCACTTCT ATCAGGGTCT GGTGTACTTG
    CTTGAAAATG ATGTCTCCAC ACTGGGATAT GACTTGACTT TCAGCACTGA GGTTCAAGAA
    TTTGGTGTCT GTGAAGTCCG AGACCTAAAA CCAAACGGTG CAAACATTCT GGTAACAGAA
    GAAAACAAAA AGGAGTATGT TCATCTTGTG TGCCAGATGA AGATGACAGG TGCAATACGT
    AAGCAATTGG CTGCCTTCCT TGAGGGATTT TATGAGATAA TCCCCAAACG TCTGATCTCC
    ATCTTCACTG AGCAGGAATT GGAACTGCTT ATCTCTGGCC TTCCCACCAT AGATATTGAT
    GACCTAAAAT CAAACACTGA GTATCACAAA TACCAGTCTA ACTCCATCCA GATTCAGTGG
    TTCTGGAGAG CGTTGCGCTC ATTCGATCAA GCTGACCGGG CAAAGTTCCT TCAGTTTGTT
    ACTGGAACTT CAAAGGTCCC ATTACAGGGT TTTGCTGCGC TGGAAGGAAT GAACGGCATC
    CAGAAATTCC AAATCCACAG AGATGATAGA TCTACAGACA GGCTGCCTTC TGCGCACACG
    TGCTTCAACC AGTTGGACCT TCCAGCCTAT GAGAGTTACG AGAAGCTACG TCACATGCTG
    CTGTTAGCCA TCCAGGAGTG CTCTGAGGGC TTTGGCCTTG CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65592
    Clone ID Related Accession (Same CDS sequence) XM_031892172.1
    Accession Version XM_031892172.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
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    1981
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    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGGCGAT ACAGCCTCTA ACCTGGGCAG TGCTGTTGAT
    GAACTGATGA GGCACCAGCC CACGTTAAAA ACAGACGCTA CAACTGCCAT CATTAAGCTC
    TTAGAAGAGA TATGTAACCT TGGTAGGGAC CCAAAATACA TCTGTCAGAA GCCATCTATT
    CAGAAAGCTG ATGGCACGGC TACTGCTCCT CCACCACGTT CCAACCATGC AGCAGAGGAG
    GCTTCTAGTG AGGATGAAGA GGAGGAGGAG GTACAAGCAA TGCAGAGTTT CAGCACAAAT
    CAGCAAAGTG AAGTTGAACC CAGCCAGCAT GTTGTTGGCA CAGAGGAGAG GATTCCTATT
    CCCCTAATGG ATTATATTCT CAATGTGATG AAATTTGTGG AATCAATTCT AAGTAACAAC
    ACAACTGATG ATCACTGCCA GGAATTTGTC AGTCAGAAGG GGCTCCTTCC ACTTGTCACC
    ATCCTTGGGC TTCCCAACCT ACCTATTGAC TTTCCTACCT CTGCTGCTTG CCAAGCTGTG
    GCAGGTGTCT GCAAATCCAT TTTGACTCTT TCTCATGAGC CAAAAGTTCT CCAAGAAGGA
    CTTCTTCAGC TGGATTCAAT TCTTTCATCT CTTGATCCAC TTCATCGGCC CATTGAGGCT
    CCTGGTGGCT CAGTACTGCT TCGAGAGTTG GCATGTGCTG GAAATGTGGC AGATGCTACT
    TTGTCTGCTC AAGCAACTCC ATTATTACAC GCTCTCACTG CAGCCCATGC CTACATCATG
    ATGTTTGTGC ATACATGCAG GGTTGGACAG AGTGAAATCC GTGCCATCTC TGTGAACCAG
    TGGGGATCAC AGCTGGGTCT CAATGTTTTG AACAAGTTGA GCCAGCTCTA CTGCTCCCTT
    GTATGGGAAA GTACAGTTTT GCTGTCACTT TGTACTCCTA ACAGTTTGCC TCCTGGGTGT
    GAGTTTGGCC AAGCAGATAT GCAGAAATTA GCCCCAAAGG AGGAAAAGGC AACCACTGTA
    TTGCCCCAAG GAACCAAAAG ACCTGAAAGT GAGGTTGAAG GCACAGTTGT CAGTGAGGAA
    GCTTCAGGCT TACTTGAAGG AATTGGACTG GATGGAGATA CTTTAGCTCC AATGGAAACT
    GATGAACCCA GCACTTCTGA TGCAAAGGGC AAACCGAAAA TAACCCCAGC TATGGCGGCC
    AGAATCAAGC AAATCAAACC ATTACTATCA GCTTCTTCTA GGTTGGGCCG GGCTTTGGCA
    GAGCTGTTTG GGTTGCTTGT CAAACTGTGT GTAGGTTCCC CTGTTCGGCA AAGGAGAAGT
    CACCATGCTG CCAGCACTAC CACAGCCCCT ACACCAGCTG CTCGCTCCAC AGCCTCTGCC
    CTCACCAAAC TGCTCACAAA AGGGCTGTCT TGGCAGCCTC CACCCTATAC CCCTACTCCT
    AGGTTCAGAT TGACTTTCTT TATCTGCTCC GTTGGGTTCA CATCCCCCAT GCTGTTTGAT
    GAAAGGAAAT ACCCATATCA CCTCATGCTG CAGAAATTCC TTTGCTCTGG TGGGCACAAT
    GCACTCTTTG AGACTTTTAA TTGGGCACTT TCAATGGGTG GAAGAGTTCC TGTTGCTGAG
    GGCCTGGAAC ACAGTGATCT TCCTGATGGT ACTGGAGAGT TTCTAGATGC TTGGCTTATG
    TTAGTGGAAA AGATGGTGAA TCCTACCACT GTGCTGGAAT CTCCTCATTC ACTTCCAACT
    ACAAAGTTAC CTGGAGGGCA GAATAATCCA CAGTTCAGTG CTTTACGCTT CCTTGTAGTC
    ACACAAAAGG CAGCTTTTAC CTGTATCAAA AACCTTTGGA ATCGGAAACC CCTGAAAGTA
    TATGGAGGGC GTATGGCAGA ATCAATGCTT GCAATCTTAT GTCATATACT TCGCGGTGAG
    CCAGTTATCC GTGAGCGTTT GAGCAAGGAG CGAGAGGGGA GCCGTGAAGA GGAGGGGCAG
    CAGGATGAGA GTGGAGCAAG ACGGGAGCCT CAAGTGAACC AACAGCAACT TCAGCAGGTG
    CTGATGGATA TGGGTTTCAC AAGGGAGCAT GCTCTGGAAG CTCTGCTTAA CACGAGTACT
    ATGGAGCAGG CCACTGAATA TCTCCTCACC CACCCTCCAC CTCTTATTGG CGGAGTTGTA
    CGGGACTTAA GTATGTCAGA GGAAGATCAG ATGCTGAGAG CCATTGCCAT GTCGCTGGGA
    CAAGATATCC CTATGGAACA GAGGGCAGAA CTGCCCGAGG AAACTGCTCG GCGGAAAGAA
    GAGGAGGAAC GCAAGGCACG AGAAAAACAA GAGGAAGAGG AAGCTAAATG CTTGGAAAAG
    TTTCAAGATG CAGAACCCCT GGAACAGGAT GAGCTTCAAG GATTCACAGA CACCATGCTC
    CCTGGCTGCT TCCACTTGCT TGATGAGCTG CCAGACACAG TCTATAGAGT GTGTGATCTT
    ATAATGACTG CCATAAAGCG CAATGGGGCA GACTATCGAG ACATGATTCT AAAACAAGTG
    GTTAACCAGG TATGGGAAGC CGCTGATGTT CTTATTAAAG CTGCACTGCC GCTGACCACA
    AGTGACACAA AAACTGTGTC TGAGTGGATC AGCCAAATGG CTACTCTTCC TCAAGCTTCT
    AACTTGGCCA CTCGCATCCT TTTGCTCACA CTGCTGTTTG AGGAGCTAAA ACTGCCATGT
    GCTAGAGTTG TTGAAACAAG TGGAATACTT AACGTACTAA TTAAGCTGCT TGAAGTGGTT
    CAGCCATGTT TGCAGGCAGC CAAGGAGCAA AAGGAAGTTC AGACACCAAA GTGGATTACA
    CCAGTCCTCC TCTTAATAGA TTTTTATGAG AAGACCTCAA TTTCTTCTAA AAGAAGAATG
    CAAATGAACA GGTACCTTCA AGCTAATGGA AACAACTGGC GATGGTTTGA TGATCGGTCT
    GGCCGTTGGT GCAGCTACAG TGCTAGTAAC AACAGCACTA TTGATGCAGC CTGGAAAGCA
    GGAGAAACAT GTGTGCGATT TACAGCTGGC CGTCGAAGAT ATACTGTGCA GTTTACCACA
    ATGGTTCAGG TAAATGAAGA GACCGGCAAT AGACGACCTG TCATGCTGAC TCTCTTGCGT
    GTTCCGAGAC TTTTAAAGAG TGGAAAAAAT GGAAATGGAC AGGAACTTGA AAAGACACTT
    GAGGAGGCAA AGGAGGTTGA AGCAAAGCAA AAAGACGAAA AAGTGCCTGA GCCCATTCCA
    GTTCCTGAAA CCTCTTCCAC TGAGAAAGAT GCACCAACTG AAGAGCCCAA AGTTGGAGAC
    ATAGTAATTC AGGGTCTGGC TGAAGAAATG GTGACCGTCC TTATACGTTC CTGCGTCGGC
    ATGCTTGGTG TTCCTGTTGA TCCAGATACT CTTCATGCAA CCTTGCGTCT TTGCCTGCGT
    CTCACACGTG ACCACAAATA TGCCATGATG TTTGCAGAGC TTAAGAGCAC TCGAATGATC
    CTCAGCTTAA CTCAAAGCTC TGGATTCAAT GGCTTTACTC CCCTTGTGAC GCTCCTATTT
    AGGCACATCA TAGAGGATCC TTGCACCCTC CAGCACACCA TGGAAAAGGT TGTACGGTCT
    GCAGCAACTA GTGGGGCAGG GAGTACCACA TCTGGGGTTG TGTCTGGAAG CCTGGGTTCG
    CGTGAAATCA ACTATATACT TAGAGTACTG GGTCCAGCAG CTTGTCGAAA CCCTGACATT
    TTCACTGAAG TGGCAAACAG TTGCATTCGC ATTGCTCTCC CTGCACCTCG TGGATCTGGA
    ACTGCCTCTG ATGATGAGTT TGAGAACCTT CGGATCAAAG GTCCTAATGC AGTTCAGTTG
    GTTAAAACCA CCCCTATAAA GCCCTCCACC TTGCCAGTCA TCCCTGATAC AATTAAGGAA
    GTAATCTATG ACATGCTGAA TGCACTGGCA GCTTACCACG CCCCTGAGGA AGTATCTGAA
    AAGTCTGAGC AAAAACCTGT TGGCATGAAC CAGGAAGTCA GCCAGATCCT ACAGGACATG
    GGTGATGAGG TTTTCCAGCA ATATCGGGAC TTAACCAGAC AGGGAAGCGA CTTTGATGGT
    CAGACCAGTT TTGCCATCAA CGCGCGAGTC TTTACTGCTG ACAGCACAGC TGCCGAAACA
    CCTCAGTCTG GTACACCTCA AGGTGAAACT GCCTTAACAC CTGAAGAAAA CCGAGAAGGG
    TTAAAGAAAG ATAAGGAAGG AGACCGTAGT TCAGAAGATG GAAAGCAGAA GGCAAAAGGT
    AGTAAGCCTT TGCTGCCTAC CTCTTCCATT CTTCGACTAC TTGCTGAACT TGTCAGGTCC
    TATGTCGGAA TAGCCTCGCT GATCGCCACC TACAATTACA CTGCAGGACA ATCTGAACTA
    ATTAAAGAGG ATTGCAGTGT ATTGGCGTTT GTGCTGGATC ACCTGCTACC CCACACACAT
    AATGCAGAGG ACAAGGACAC ACCAGCCCTA GCCCGCCTCT TCTTAGCCAG CCTTGCTGCA
    GCAGGAAGTG GCACTGATGC CCAGGTTGCA CTAGTGAATG AAGTAAAGGC AGCTCTTGGC
    AGAGCCCTAG CAATGGCTGA GAGCACGGAG AAGCATGCTA GGTTGCAGGC TGTGATGTGC
    ATTATCAGTA CCATTATGGA GTCATGTCCA TCCACCTCCA GCTTCTACAG CACAGCAACC
    ACCAAAACCC AGCACAATGG CATGAACAAC ATCATCAGAC TCTTTTTGAA AAAGGGGCTG
    GTTAGTGATT TAGCCAGGGT CCCTCACAGC CTTGATTTAT CCAGTCCAAA TATGGCAAAC
    ACCGTGAATG CAGCACTGAA GCCACTCGAG ACACTGTCTC GCATTGTCAA TCAACCCAGC
    AGTTTGTTTG GAAGCAAAGG GACTTCAGGA AAGAATAAAT CTGAGGCAGA TACACATGGA
    ACAGCCCAGG ATCCCAACAG TAATCAGCCA GATACAGGTG AAACAGGAAC AGAAGTGCAG
    GAAGAGGACC CAGATGCTTC TCATGCTGAA GTAGCTGATG GAGACATCAT GGATGGAGAG
    ACAGAGACTG ATGCTGTGGT TATTGCTGGC CAGCCTGAAG TCCTTACTAC ACAAGAAATG
    CAGGTAGAGA ATGAGCTTGA AGATCTGATA GATGAACTTC TTGAAAGGGA TGGTGCTGCA
    GGTAACCGGG TCGGAGAAGA TGAATCACAG GAAGATGTGC TGATGGATGA GCCTCCTACT
    AACCTCAGCC AAGCCTCCAC TCTTCAGGCT AATCGAGAAG ACTCTATGAA CATCTTAGAC
    CCAGAGGATG AGGAAGAGCA CACCCAAGAA GAGGACAGCA GTGGCAGCAA TGAAGATGAA
    GATGACAGCC AGGAAGAGGA GGAGGAAGAG GAAGATGAGG ACGATCAGGA TGATGAGGAG
    GGTGAGGAAG GGGATGAGGA TGATGATGAT GACTGTTCAG AGATGGAACT GGATGAGGAT
    TATCCAGACA TGAATGCCTC ACCTCTTGTA CGCTTTGAAC GTTTTGACAG AGAAGATGAT
    TTAATCATAG AGTTTGATAA CATGTTCTCC AGTGCCACTG AGATCCCTCC ATCCCCTGGT
    AACATCCCTA CTACACACCC CCTAATGGTG CGACACGCTG ATCACGGCTC CTTGACTCTT
    GGCAGTGGCA CTTCCACTAC TCGCCTGGCA CAGGGCATCG GGCGCAGCCA GAGAACACTC
    CGACAACTCA CAGCCAACAC CGGTCACACA ATACATGTTC ATTATCCTGG CAACAGGCAG
    CCAAATCCAC CTTTAATCCT CCAGAGGTTG CTGGGGCCCT CAGCCGCTGC TGACATCTTG
    CAACTAAGCA GCAGTCTTCC TCTGCAGAGT CGTGGCCGAG CACGTCTACT TGTTGGCAAC
    GATGATGTCC ACATCATAGC ACGCTCTGAT GATGAGATGC TTGATGATTT TTTCCATGAT
    CAGAGTACAG CCACAAGTCA GGCTGGCACA CTTTCCAGCA TTCCAACTGC GCTGACCCGC
    TGGACAGAAG AGTGTAAGGT CTTGGATGCT GAAAGCATGC ATGACTGTGT ATCTGTGGTC
    AAAGTCCCAA TCATCAATCA TTTGGAATCT TTGCGTGATG AGGAGCTTGA GGAGCGCAGG
    GAAAAAAGAA GGAAACAACT AGCAGAAGAG GAGGCCAAGA TGGCAGAGAA ATCCAAGGAG
    GAGAAGGAAG CAAAGGAGCA AAATTCACAG GGTGCCAATT CAAAGCAGAA TACCCCATTT
    GAACTGAGCC TTGCAGATCA AGATGGAAGT GGTCGTCCTG AAAATTTCCC AACTGCACCA
    TCTTCTGCAG AAGGAACTCC AACAAGTCCT GAAATTGTTC GTTTAGATCC CACCCTAAGG
    GCTACTGAGG CTACTCTGGC AGAGCTGACT CAGGGTCTGC CTGCTGATAC AGGCACGGAG
    GATGTTGGGC AACAACTCAT GCCATCTCAC CAAGAGGACT CTGGACCAGG ACGGCCAAGT
    GTAGAGGGGG ATGCCACACA AATGGAGCTA TCGCCAGCAC CCACTATAAC CTCCTTATCA
    CCAGAGCGAG CAGAGGATTC AGATGCTTTG ACAGCTGTTA GTAGCCAACT AGAGGGCTCG
    CCTATGGACA CCAGCAGTCT TGCCTCTGGG CCCTTAGAGG ATGGCGTAGG GGAGGCACCT
    GGAATTGTTA ATCCAGAGCA GCCTCTTGTG GTTGGCAGTA GCATTGCTGT TGGACAGCAA
    CCAGTGGAAC CACCGACGAC CTGTGAAACC CAACCAGTAG TTTCTCAACA CAGTGATAGC
    TCACAAATTG CCCCTTCCTC TGAGTTGCCT TCCACAAGAG AAAGTGCTGT GACCTTGTTG
    GCTACAGATT CTAGTGGTAT CCTTGAAGAA CCTTTGCCTT CTACAAGCAG TGAAGAGGAA
    GACCCATTAG CAGGCATAAA TCTTCCAGAG GGTGTGGATC CTTCTTTCCT AGCGGCTCTT
    CCTGAAGACA TCCGCAGAGA GGTGCTGCAG AATCAGTTGG GCATACGGCC GCCATCGCGA
    CCTCCACCCA CTGCTGCCCC AGTTATTCCT CCTCCTGTGG TTGCTAATGC TGGTGTCACG
    GAGGTCAGCC CTGAGTTCTT GGCAGCGTTG CCACCAGCCA TTCAGGAAGA GGTCCTAGCA
    CAGCAGCGAG CAGAACAGCA GAGGCGGGAA CTTGCTCAGA CCACTACGTC TGATACACCC
    ATGGATCCTG TGACTTTCAT TCAGACGTTA CCTTCTGATC TACGTCGTAG TGTGTTAGAA
    GACATGGAGG ACAGTGTTTT AGCTGTGATG CCACCTGACA TTGCTGCAGA GGCACAGGCT
    CTAAGGCGAG AGCAAGAAGC TCGGCAGAGG CAACTAATGC ATGAACGACT GTTCGGGCAC
    AGTAGCACAT CTGCACTCTC TGCCATCCTT AGGAGTCCTG CTTTCACGAG TCGCTTGAGT
    GGAAACCGAG GTGTTCAGTA CACACGGTTG GCTGTACAGA GAGGGAACAC TTTCCAAATG
    GGAAGCAGCA GTACACATAC CAGACCAACC GGTAGTAATG TTGACAGCCT ACTACGATTA
    CGAGGCCGAC TATTACTTGA TCATGAAGCC CTCTCCTGTC TGCTTGTCCT ACTGTTTGTT
    GATGAACCCA AGCTAAACAC CAGTCGCTTG CACCGTGTTC TACGCAACCT CTGCTACCAT
    GCACAAACAA GGAACTGGGT GATTAGAAGC TTGTTGTCTA TACTGCAGCG TAGCAGTGAA
    AGTGAGCTTT GTATAGAAAG CCCAAAGACT TCTATTGGGG GCACTGATAA ACGGGGTGCA
    AAAACAAGTC ATGACAACCG CCCTTTAGAA TTTCTGCATA AGATGGAATC GAAGAATTCC
    AACCAGCTCT CCTGGCTTTC TGTATCCATG GACGCTGCTC TTGGCTGCCG TACGAACATC
    TTCCAAATCC AGAGGTCCCT AGGACGCAAG CATACAGAAA AGCATTCAGC CTGTGGTTCT
    ACTGTCCACA TCCATCCCCA AGCAGCCCCT GTAGTGTGTA GACATGTGCT TGACACACTA
    ATACAGCTAG CTAAGGTGTT TCCCAGTCAT TTCACACAGC AGCGAACCAA AGAATGTAGT
    GTGGAAAACA GTGACAGCAG GGAGCGGAGT ATAAAACTTA CTTGTAGTCC GTGTCTTGCA
    CCATCTAGTA GTATTGGAGC AAGCAACATC TCCACAGATT TCTGGGACTT GCTAGTCAAG
    CTAGACAACA TGAATGTGAG CCGGAAGGGG AAAACGTCTA TAAAATCTGT GCCAGTGAGC
    TCTGGTTCTG AAGGCGAGAG CAATCAGTGC AGCCTGGAGG CCTCCCCCTT AGGACAGCTT
    ATGAATATGC TGTCTCATTC TGTTATTCGC CGCAGTTCGC TGCTTACTGA AAAGCTGCTG
    AGACTTCTCT CCCTTATCTC TATTGCCCTT CCTGATAATA AGGCTTCTGA AACAGCAGGC
    AATCAGAGCA ATTCTATTAC CCCAGCACCT GCACCTACGC CTGCAACTGC ACCAGCCCCT
    GTGCCTGTAC CTGCTGTAAC AGTTCCAGTT GTTGTGAGCA CAAACATAGT GACAACTGTT
    ACAACCAAAA GTGGAAAATC TCCTGCAAAA TCAAGTGAAG TCAGCAATGC ACCAGATAAG
    ATGGCAGCTT CTGGCCTTAC AGAGAAACAG CTGCAGTTGT CTGTTGAGGT TCTGACATCA
    CACTCGTGTT CAGAAGAAGG ACTGGAGGAT GCCGCCAATG TTCTACTTCA GCTTTCCAGA
    GGCGACCCCA GTACACGGGA CACTGTACTG AAACTTCTGC TTAGCGGAGC ACATCACTTG
    GGGTTTACTC TCTGCAAACA GATAGGTACA CTACTAGCAG AGCTCCGGGA ATATAACCTA
    GAGCAGCAGC GTCGTGCACA GTGTGAAGTT CTTTCTCCAG ATGGTATGCC TGAGGAGCAA
    CCCCACAGCA CAAAGCCAAA AGGCAAAATG CAGAGCAGTG TTAGGTCTCT CCCCACCCCA
    CCTATGCGAT CCAGGTTCGA CATTGCAGAG AATGTGGTGA TTGTGGCTTC CCAAAAGCGT
    CCACTGGGAG GAAGAGAGCT GCAGCTCCCC TCAATGTCTA TGCTGACGTC AAAGACTTCC
    ACACAAAAAT TCTTTCTTCG AGTGCTGCAG GTTATCATCC AGCTTCGCGA TGACACTCGC
    AGAGCCAACA AGAAGGCCAA GCAGACCAGC AGATTAGGCA CTGCCAGTCT GGCCTCGGCT
    AGCAGCATCC AAGCAGCTGT GCAGCAGCTG GAGGCTGAGG CTGATGCCAT TATACAAATG
    GTACGTGAGG GTCAAAGGGC GCGGAGACAG CTGCAAACTG CAACGGCGGA GGGTTCTGCT
    CGCAGGGAGG AGCAGCCTAT GGATGTTGAC CAGCCTTCGC CTGCTACACA GGAGCCTCAG
    TCAGTTGCTT CTGAAGGTTC TCAGGCTGCT GAAAAAAAAG AAGAGGAGAG GTTACCAGAG
    TTACCGCTGT TAAGTGAGCA GCTAAGTTTA GATGAGCTGT GGGACATGTT GGGAGAGTGC
    CTGAAGGAAC TTGAGGAATC TCATGACCAG CATGCCGTAT TAGTGTTACA GCCAGCCGTA
    GAAGCCTTTT TCCTTGTTCA TGCAACAGAG CGGGAGAGCA AGCCTCCTGT GCGGGACACT
    CGGGAAAGCC AGTTATCTCA CATTAAGGAT GAACCACCTC CCCTGTCTCC TGCACCCCTC
    ACTCCAGCCA CACCTTCTTC CTTGGATCCT TTCTTCTCTC GTGAACCTTC ATCTATGCAC
    ATTTCCTCTA GTTTGCCTCC TGACACTCAG AAGTTCCTAA GATTTGCTGA GACTCATCGG
    ACTGTATTAA ATCAGATCCT TCGCCAGTCC ACCACACACC TAGCTGATGG GCCCTTTGCT
    GTGCTTGTAG ACTATATCAG AGTCCTTGAC TTTGATGTTA AGCGCAAATA TTTCCGACAG
    GAGTTGGAGC GACTGGATGA AGGGCTAAGA AAAGAGGATA TGGCAGTGCA TGTGCGGCGA
    GATCATGTGT TTGAGGATTC CTACCGGGAG CTGCACCGCA AATCCCCTGA AGAAATGAAG
    AATAGATTGT ACATAGTATT TGAAGGAGAA GAAGGGCAGG ATGCAGGTGG GCTGCTTCGG
    GAGTGGTACA TGATTATCTC CAGGGAGATG TTCAATCCCA TGTATGCTTT GTTCCGCACT
    TCCCCAGGTG ACCGCGTCAC CTACACTATT AATCCTTCCT CCCACTGTAA CCCTAACCAC
    CTCAGCTACT TCAAGTTTGT GGGCCGGATA GTTGCCAAAG CGGTTTATGA CAACCGGCTA
    CTGGAGTGTT ACTTCACTAG GTCCTTCTAC AAGCATATAT TGGGCAAGTC TGTCAGATAC
    ACAGACATGG AGAGTGAAGA TTATCACTTC TATCAGGGTC TGGTGTACTT GCTTGAAAAT
    GATGTCTCCA CACTGGGATA TGACTTGACT TTCAGCACTG AGGTTCAAGA ATTTGGTGTC
    TGTGAAGTCC GAGACCTAAA ACCAAACGGT GCAAACATTC TGGTAACAGA AGAAAACAAA
    AAGGAGTATG TTCATCTTGT GTGCCAGATG AAGATGACAG GTGCAATACG TAAGCAATTG
    GCTGCCTTCC TTGAGGGATT TTATGAGATA ATCCCCAAAC GTCTGATCTC CATCTTCACT
    GAGCAGGAAT TGGAACTGCT TATCTCTGGC CTTCCCACCA TAGATATTGA TGACCTAAAA
    TCAAACACTG AGTATCACAA ATACCAGTCT AACTCCATCC AGATTCAGTG GTTCTGGAGA
    GCGTTGCGCT CATTCGATCA AGCTGACCGG GCAAAGTTCC TTCAGTTTGT TACTGGAACT
    TCAAAGGTCC CATTACAGGG TTTTGCTGCG CTGGAAGGAA TGAACGGCAT CCAGAAATTC
    CAAATCCACA GAGATGATAG ATCTACAGAC AGGCTGCCTT CTGCGCACAC GTGCTTCAAC
    CAGTTGGACC TTCCAGCCTA TGAGAGTTAC GAGAAGCTAC GTCACATGCT GCTGTTAGCC
    ATCCAGGAGT GCTCTGAGGG CTTTGGCCTT GCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748032.1
    CDS671..13786
    Translation

    Target ORF information:

    RefSeq Version XM_031892172.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892172.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGGCGAT ACAGCCTCTA ACCTGGGCAG TGCTGTTGAT
    GAACTGATGA GGCACCAGCC CACGTTAAAA ACAGACGCTA CAACTGCCAT CATTAAGCTC
    TTAGAAGAGA TATGTAACCT TGGTAGGGAC CCAAAATACA TCTGTCAGAA GCCATCTATT
    CAGAAAGCTG ATGGCACGGC TACTGCTCCT CCACCACGTT CCAACCATGC AGCAGAGGAG
    GCTTCTAGTG AGGATGAAGA GGAGGAGGAG GTACAAGCAA TGCAGAGTTT CAGCACAAAT
    CAGCAAAGTG AAGTTGAACC CAGCCAGCAT GTTGTTGGCA CAGAGGAGAG GATTCCTATT
    CCCCTAATGG ATTATATTCT CAATGTGATG AAATTTGTGG AATCAATTCT AAGTAACAAC
    ACAACTGATG ATCACTGCCA GGAATTTGTC AGTCAGAAGG GGCTCCTTCC ACTTGTCACC
    ATCCTTGGGC TTCCCAACCT ACCTATTGAC TTTCCTACCT CTGCTGCTTG CCAAGCTGTG
    GCAGGTGTCT GCAAATCCAT TTTGACTCTT TCTCATGAGC CAAAAGTTCT CCAAGAAGGA
    CTTCTTCAGC TGGATTCAAT TCTTTCATCT CTTGATCCAC TTCATCGGCC CATTGAGGCT
    CCTGGTGGCT CAGTACTGCT TCGAGAGTTG GCATGTGCTG GAAATGTGGC AGATGCTACT
    TTGTCTGCTC AAGCAACTCC ATTATTACAC GCTCTCACTG CAGCCCATGC CTACATCATG
    ATGTTTGTGC ATACATGCAG GGTTGGACAG AGTGAAATCC GTGCCATCTC TGTGAACCAG
    TGGGGATCAC AGCTGGGTCT CAATGTTTTG AACAAGTTGA GCCAGCTCTA CTGCTCCCTT
    GTATGGGAAA GTACAGTTTT GCTGTCACTT TGTACTCCTA ACAGTTTGCC TCCTGGGTGT
    GAGTTTGGCC AAGCAGATAT GCAGAAATTA GCCCCAAAGG AGGAAAAGGC AACCACTGTA
    TTGCCCCAAG GAACCAAAAG ACCTGAAAGT GAGGTTGAAG GCACAGTTGT CAGTGAGGAA
    GCTTCAGGCT TACTTGAAGG AATTGGACTG GATGGAGATA CTTTAGCTCC AATGGAAACT
    GATGAACCCA GCACTTCTGA TGCAAAGGGC AAACCGAAAA TAACCCCAGC TATGGCGGCC
    AGAATCAAGC AAATCAAACC ATTACTATCA GCTTCTTCTA GGTTGGGCCG GGCTTTGGCA
    GAGCTGTTTG GGTTGCTTGT CAAACTGTGT GTAGGTTCCC CTGTTCGGCA AAGGAGAAGT
    CACCATGCTG CCAGCACTAC CACAGCCCCT ACACCAGCTG CTCGCTCCAC AGCCTCTGCC
    CTCACCAAAC TGCTCACAAA AGGGCTGTCT TGGCAGCCTC CACCCTATAC CCCTACTCCT
    AGGTTCAGAT TGACTTTCTT TATCTGCTCC GTTGGGTTCA CATCCCCCAT GCTGTTTGAT
    GAAAGGAAAT ACCCATATCA CCTCATGCTG CAGAAATTCC TTTGCTCTGG TGGGCACAAT
    GCACTCTTTG AGACTTTTAA TTGGGCACTT TCAATGGGTG GAAGAGTTCC TGTTGCTGAG
    GGCCTGGAAC ACAGTGATCT TCCTGATGGT ACTGGAGAGT TTCTAGATGC TTGGCTTATG
    TTAGTGGAAA AGATGGTGAA TCCTACCACT GTGCTGGAAT CTCCTCATTC ACTTCCAACT
    ACAAAGTTAC CTGGAGGGCA GAATAATCCA CAGTTCAGTG CTTTACGCTT CCTTGTAGTC
    ACACAAAAGG CAGCTTTTAC CTGTATCAAA AACCTTTGGA ATCGGAAACC CCTGAAAGTA
    TATGGAGGGC GTATGGCAGA ATCAATGCTT GCAATCTTAT GTCATATACT TCGCGGTGAG
    CCAGTTATCC GTGAGCGTTT GAGCAAGGAG CGAGAGGGGA GCCGTGAAGA GGAGGGGCAG
    CAGGATGAGA GTGGAGCAAG ACGGGAGCCT CAAGTGAACC AACAGCAACT TCAGCAGGTG
    CTGATGGATA TGGGTTTCAC AAGGGAGCAT GCTCTGGAAG CTCTGCTTAA CACGAGTACT
    ATGGAGCAGG CCACTGAATA TCTCCTCACC CACCCTCCAC CTCTTATTGG CGGAGTTGTA
    CGGGACTTAA GTATGTCAGA GGAAGATCAG ATGCTGAGAG CCATTGCCAT GTCGCTGGGA
    CAAGATATCC CTATGGAACA GAGGGCAGAA CTGCCCGAGG AAACTGCTCG GCGGAAAGAA
    GAGGAGGAAC GCAAGGCACG AGAAAAACAA GAGGAAGAGG AAGCTAAATG CTTGGAAAAG
    TTTCAAGATG CAGAACCCCT GGAACAGGAT GAGCTTCAAG GATTCACAGA CACCATGCTC
    CCTGGCTGCT TCCACTTGCT TGATGAGCTG CCAGACACAG TCTATAGAGT GTGTGATCTT
    ATAATGACTG CCATAAAGCG CAATGGGGCA GACTATCGAG ACATGATTCT AAAACAAGTG
    GTTAACCAGG TATGGGAAGC CGCTGATGTT CTTATTAAAG CTGCACTGCC GCTGACCACA
    AGTGACACAA AAACTGTGTC TGAGTGGATC AGCCAAATGG CTACTCTTCC TCAAGCTTCT
    AACTTGGCCA CTCGCATCCT TTTGCTCACA CTGCTGTTTG AGGAGCTAAA ACTGCCATGT
    GCTAGAGTTG TTGAAACAAG TGGAATACTT AACGTACTAA TTAAGCTGCT TGAAGTGGTT
    CAGCCATGTT TGCAGGCAGC CAAGGAGCAA AAGGAAGTTC AGACACCAAA GTGGATTACA
    CCAGTCCTCC TCTTAATAGA TTTTTATGAG AAGACCTCAA TTTCTTCTAA AAGAAGAATG
    CAAATGAACA GGTACCTTCA AGCTAATGGA AACAACTGGC GATGGTTTGA TGATCGGTCT
    GGCCGTTGGT GCAGCTACAG TGCTAGTAAC AACAGCACTA TTGATGCAGC CTGGAAAGCA
    GGAGAAACAT GTGTGCGATT TACAGCTGGC CGTCGAAGAT ATACTGTGCA GTTTACCACA
    ATGGTTCAGG TAAATGAAGA GACCGGCAAT AGACGACCTG TCATGCTGAC TCTCTTGCGT
    GTTCCGAGAC TTTTAAAGAG TGGAAAAAAT GGAAATGGAC AGGAACTTGA AAAGACACTT
    GAGGAGGCAA AGGAGGTTGA AGCAAAGCAA AAAGACGAAA AAGTGCCTGA GCCCATTCCA
    GTTCCTGAAA CCTCTTCCAC TGAGAAAGAT GCACCAACTG AAGAGCCCAA AGTTGGAGAC
    ATAGTAATTC AGGGTCTGGC TGAAGAAATG GTGACCGTCC TTATACGTTC CTGCGTCGGC
    ATGCTTGGTG TTCCTGTTGA TCCAGATACT CTTCATGCAA CCTTGCGTCT TTGCCTGCGT
    CTCACACGTG ACCACAAATA TGCCATGATG TTTGCAGAGC TTAAGAGCAC TCGAATGATC
    CTCAGCTTAA CTCAAAGCTC TGGATTCAAT GGCTTTACTC CCCTTGTGAC GCTCCTATTT
    AGGCACATCA TAGAGGATCC TTGCACCCTC CAGCACACCA TGGAAAAGGT TGTACGGTCT
    GCAGCAACTA GTGGGGCAGG GAGTACCACA TCTGGGGTTG TGTCTGGAAG CCTGGGTTCG
    CGTGAAATCA ACTATATACT TAGAGTACTG GGTCCAGCAG CTTGTCGAAA CCCTGACATT
    TTCACTGAAG TGGCAAACAG TTGCATTCGC ATTGCTCTCC CTGCACCTCG TGGATCTGGA
    ACTGCCTCTG ATGATGAGTT TGAGAACCTT CGGATCAAAG GTCCTAATGC AGTTCAGTTG
    GTTAAAACCA CCCCTATAAA GCCCTCCACC TTGCCAGTCA TCCCTGATAC AATTAAGGAA
    GTAATCTATG ACATGCTGAA TGCACTGGCA GCTTACCACG CCCCTGAGGA AGTATCTGAA
    AAGTCTGAGC AAAAACCTGT TGGCATGAAC CAGGAAGTCA GCCAGATCCT ACAGGACATG
    GGTGATGAGG TTTTCCAGCA ATATCGGGAC TTAACCAGAC AGGGAAGCGA CTTTGATGGT
    CAGACCAGTT TTGCCATCAA CGCGCGAGTC TTTACTGCTG ACAGCACAGC TGCCGAAACA
    CCTCAGTCTG GTACACCTCA AGGTGAAACT GCCTTAACAC CTGAAGAAAA CCGAGAAGGG
    TTAAAGAAAG ATAAGGAAGG AGACCGTAGT TCAGAAGATG GAAAGCAGAA GGCAAAAGGT
    AGTAAGCCTT TGCTGCCTAC CTCTTCCATT CTTCGACTAC TTGCTGAACT TGTCAGGTCC
    TATGTCGGAA TAGCCTCGCT GATCGCCACC TACAATTACA CTGCAGGACA ATCTGAACTA
    ATTAAAGAGG ATTGCAGTGT ATTGGCGTTT GTGCTGGATC ACCTGCTACC CCACACACAT
    AATGCAGAGG ACAAGGACAC ACCAGCCCTA GCCCGCCTCT TCTTAGCCAG CCTTGCTGCA
    GCAGGAAGTG GCACTGATGC CCAGGTTGCA CTAGTGAATG AAGTAAAGGC AGCTCTTGGC
    AGAGCCCTAG CAATGGCTGA GAGCACGGAG AAGCATGCTA GGTTGCAGGC TGTGATGTGC
    ATTATCAGTA CCATTATGGA GTCATGTCCA TCCACCTCCA GCTTCTACAG CACAGCAACC
    ACCAAAACCC AGCACAATGG CATGAACAAC ATCATCAGAC TCTTTTTGAA AAAGGGGCTG
    GTTAGTGATT TAGCCAGGGT CCCTCACAGC CTTGATTTAT CCAGTCCAAA TATGGCAAAC
    ACCGTGAATG CAGCACTGAA GCCACTCGAG ACACTGTCTC GCATTGTCAA TCAACCCAGC
    AGTTTGTTTG GAAGCAAAGG GACTTCAGGA AAGAATAAAT CTGAGGCAGA TACACATGGA
    ACAGCCCAGG ATCCCAACAG TAATCAGCCA GATACAGGTG AAACAGGAAC AGAAGTGCAG
    GAAGAGGACC CAGATGCTTC TCATGCTGAA GTAGCTGATG GAGACATCAT GGATGGAGAG
    ACAGAGACTG ATGCTGTGGT TATTGCTGGC CAGCCTGAAG TCCTTACTAC ACAAGAAATG
    CAGGTAGAGA ATGAGCTTGA AGATCTGATA GATGAACTTC TTGAAAGGGA TGGTGCTGCA
    GGTAACCGGG TCGGAGAAGA TGAATCACAG GAAGATGTGC TGATGGATGA GCCTCCTACT
    AACCTCAGCC AAGCCTCCAC TCTTCAGGCT AATCGAGAAG ACTCTATGAA CATCTTAGAC
    CCAGAGGATG AGGAAGAGCA CACCCAAGAA GAGGACAGCA GTGGCAGCAA TGAAGATGAA
    GATGACAGCC AGGAAGAGGA GGAGGAAGAG GAAGATGAGG ACGATCAGGA TGATGAGGAG
    GGTGAGGAAG GGGATGAGGA TGATGATGAT GACTGTTCAG AGATGGAACT GGATGAGGAT
    TATCCAGACA TGAATGCCTC ACCTCTTGTA CGCTTTGAAC GTTTTGACAG AGAAGATGAT
    TTAATCATAG AGTTTGATAA CATGTTCTCC AGTGCCACTG AGATCCCTCC ATCCCCTGGT
    AACATCCCTA CTACACACCC CCTAATGGTG CGACACGCTG ATCACGGCTC CTTGACTCTT
    GGCAGTGGCA CTTCCACTAC TCGCCTGGCA CAGGGCATCG GGCGCAGCCA GAGAACACTC
    CGACAACTCA CAGCCAACAC CGGTCACACA ATACATGTTC ATTATCCTGG CAACAGGCAG
    CCAAATCCAC CTTTAATCCT CCAGAGGTTG CTGGGGCCCT CAGCCGCTGC TGACATCTTG
    CAACTAAGCA GCAGTCTTCC TCTGCAGAGT CGTGGCCGAG CACGTCTACT TGTTGGCAAC
    GATGATGTCC ACATCATAGC ACGCTCTGAT GATGAGATGC TTGATGATTT TTTCCATGAT
    CAGAGTACAG CCACAAGTCA GGCTGGCACA CTTTCCAGCA TTCCAACTGC GCTGACCCGC
    TGGACAGAAG AGTGTAAGGT CTTGGATGCT GAAAGCATGC ATGACTGTGT ATCTGTGGTC
    AAAGTCCCAA TCATCAATCA TTTGGAATCT TTGCGTGATG AGGAGCTTGA GGAGCGCAGG
    GAAAAAAGAA GGAAACAACT AGCAGAAGAG GAGGCCAAGA TGGCAGAGAA ATCCAAGGAG
    GAGAAGGAAG CAAAGGAGCA AAATTCACAG GGTGCCAATT CAAAGCAGAA TACCCCATTT
    GAACTGAGCC TTGCAGATCA AGATGGAAGT GGTCGTCCTG AAAATTTCCC AACTGCACCA
    TCTTCTGCAG AAGGAACTCC AACAAGTCCT GAAATTGTTC GTTTAGATCC CACCCTAAGG
    GCTACTGAGG CTACTCTGGC AGAGCTGACT CAGGGTCTGC CTGCTGATAC AGGCACGGAG
    GATGTTGGGC AACAACTCAT GCCATCTCAC CAAGAGGACT CTGGACCAGG ACGGCCAAGT
    GTAGAGGGGG ATGCCACACA AATGGAGCTA TCGCCAGCAC CCACTATAAC CTCCTTATCA
    CCAGAGCGAG CAGAGGATTC AGATGCTTTG ACAGCTGTTA GTAGCCAACT AGAGGGCTCG
    CCTATGGACA CCAGCAGTCT TGCCTCTGGG CCCTTAGAGG ATGGCGTAGG GGAGGCACCT
    GGAATTGTTA ATCCAGAGCA GCCTCTTGTG GTTGGCAGTA GCATTGCTGT TGGACAGCAA
    CCAGTGGAAC CACCGACGAC CTGTGAAACC CAACCAGTAG TTTCTCAACA CAGTGATAGC
    TCACAAATTG CCCCTTCCTC TGAGTTGCCT TCCACAAGAG AAAGTGCTGT GACCTTGTTG
    GCTACAGATT CTAGTGGTAT CCTTGAAGAA CCTTTGCCTT CTACAAGCAG TGAAGAGGAA
    GACCCATTAG CAGGCATAAA TCTTCCAGAG GGTGTGGATC CTTCTTTCCT AGCGGCTCTT
    CCTGAAGACA TCCGCAGAGA GGTGCTGCAG AATCAGTTGG GCATACGGCC GCCATCGCGA
    CCTCCACCCA CTGCTGCCCC AGTTATTCCT CCTCCTGTGG TTGCTAATGC TGGTGTCACG
    GAGGTCAGCC CTGAGTTCTT GGCAGCGTTG CCACCAGCCA TTCAGGAAGA GGTCCTAGCA
    CAGCAGCGAG CAGAACAGCA GAGGCGGGAA CTTGCTCAGA CCACTACGTC TGATACACCC
    ATGGATCCTG TGACTTTCAT TCAGACGTTA CCTTCTGATC TACGTCGTAG TGTGTTAGAA
    GACATGGAGG ACAGTGTTTT AGCTGTGATG CCACCTGACA TTGCTGCAGA GGCACAGGCT
    CTAAGGCGAG AGCAAGAAGC TCGGCAGAGG CAACTAATGC ATGAACGACT GTTCGGGCAC
    AGTAGCACAT CTGCACTCTC TGCCATCCTT AGGAGTCCTG CTTTCACGAG TCGCTTGAGT
    GGAAACCGAG GTGTTCAGTA CACACGGTTG GCTGTACAGA GAGGGAACAC TTTCCAAATG
    GGAAGCAGCA GTACACATAC CAGACCAACC GGTAGTAATG TTGACAGCCT ACTACGATTA
    CGAGGCCGAC TATTACTTGA TCATGAAGCC CTCTCCTGTC TGCTTGTCCT ACTGTTTGTT
    GATGAACCCA AGCTAAACAC CAGTCGCTTG CACCGTGTTC TACGCAACCT CTGCTACCAT
    GCACAAACAA GGAACTGGGT GATTAGAAGC TTGTTGTCTA TACTGCAGCG TAGCAGTGAA
    AGTGAGCTTT GTATAGAAAG CCCAAAGACT TCTATTGGGG GCACTGATAA ACGGGGTGCA
    AAAACAAGTC ATGACAACCG CCCTTTAGAA TTTCTGCATA AGATGGAATC GAAGAATTCC
    AACCAGCTCT CCTGGCTTTC TGTATCCATG GACGCTGCTC TTGGCTGCCG TACGAACATC
    TTCCAAATCC AGAGGTCCCT AGGACGCAAG CATACAGAAA AGCATTCAGC CTGTGGTTCT
    ACTGTCCACA TCCATCCCCA AGCAGCCCCT GTAGTGTGTA GACATGTGCT TGACACACTA
    ATACAGCTAG CTAAGGTGTT TCCCAGTCAT TTCACACAGC AGCGAACCAA AGAATGTAGT
    GTGGAAAACA GTGACAGCAG GGAGCGGAGT ATAAAACTTA CTTGTAGTCC GTGTCTTGCA
    CCATCTAGTA GTATTGGAGC AAGCAACATC TCCACAGATT TCTGGGACTT GCTAGTCAAG
    CTAGACAACA TGAATGTGAG CCGGAAGGGG AAAACGTCTA TAAAATCTGT GCCAGTGAGC
    TCTGGTTCTG AAGGCGAGAG CAATCAGTGC AGCCTGGAGG CCTCCCCCTT AGGACAGCTT
    ATGAATATGC TGTCTCATTC TGTTATTCGC CGCAGTTCGC TGCTTACTGA AAAGCTGCTG
    AGACTTCTCT CCCTTATCTC TATTGCCCTT CCTGATAATA AGGCTTCTGA AACAGCAGGC
    AATCAGAGCA ATTCTATTAC CCCAGCACCT GCACCTACGC CTGCAACTGC ACCAGCCCCT
    GTGCCTGTAC CTGCTGTAAC AGTTCCAGTT GTTGTGAGCA CAAACATAGT GACAACTGTT
    ACAACCAAAA GTGGAAAATC TCCTGCAAAA TCAAGTGAAG TCAGCAATGC ACCAGATAAG
    ATGGCAGCTT CTGGCCTTAC AGAGAAACAG CTGCAGTTGT CTGTTGAGGT TCTGACATCA
    CACTCGTGTT CAGAAGAAGG ACTGGAGGAT GCCGCCAATG TTCTACTTCA GCTTTCCAGA
    GGCGACCCCA GTACACGGGA CACTGTACTG AAACTTCTGC TTAGCGGAGC ACATCACTTG
    GGGTTTACTC TCTGCAAACA GATAGGTACA CTACTAGCAG AGCTCCGGGA ATATAACCTA
    GAGCAGCAGC GTCGTGCACA GTGTGAAGTT CTTTCTCCAG ATGGTATGCC TGAGGAGCAA
    CCCCACAGCA CAAAGCCAAA AGGCAAAATG CAGAGCAGTG TTAGGTCTCT CCCCACCCCA
    CCTATGCGAT CCAGGTTCGA CATTGCAGAG AATGTGGTGA TTGTGGCTTC CCAAAAGCGT
    CCACTGGGAG GAAGAGAGCT GCAGCTCCCC TCAATGTCTA TGCTGACGTC AAAGACTTCC
    ACACAAAAAT TCTTTCTTCG AGTGCTGCAG GTTATCATCC AGCTTCGCGA TGACACTCGC
    AGAGCCAACA AGAAGGCCAA GCAGACCAGC AGATTAGGCA CTGCCAGTCT GGCCTCGGCT
    AGCAGCATCC AAGCAGCTGT GCAGCAGCTG GAGGCTGAGG CTGATGCCAT TATACAAATG
    GTACGTGAGG GTCAAAGGGC GCGGAGACAG CTGCAAACTG CAACGGCGGA GGGTTCTGCT
    CGCAGGGAGG AGCAGCCTAT GGATGTTGAC CAGCCTTCGC CTGCTACACA GGAGCCTCAG
    TCAGTTGCTT CTGAAGGTTC TCAGGCTGCT GAAAAAAAAG AAGAGGAGAG GTTACCAGAG
    TTACCGCTGT TAAGTGAGCA GCTAAGTTTA GATGAGCTGT GGGACATGTT GGGAGAGTGC
    CTGAAGGAAC TTGAGGAATC TCATGACCAG CATGCCGTAT TAGTGTTACA GCCAGCCGTA
    GAAGCCTTTT TCCTTGTTCA TGCAACAGAG CGGGAGAGCA AGCCTCCTGT GCGGGACACT
    CGGGAAAGCC AGTTATCTCA CATTAAGGAT GAACCACCTC CCCTGTCTCC TGCACCCCTC
    ACTCCAGCCA CACCTTCTTC CTTGGATCCT TTCTTCTCTC GTGAACCTTC ATCTATGCAC
    ATTTCCTCTA GTTTGCCTCC TGACACTCAG AAGTTCCTAA GATTTGCTGA GACTCATCGG
    ACTGTATTAA ATCAGATCCT TCGCCAGTCC ACCACACACC TAGCTGATGG GCCCTTTGCT
    GTGCTTGTAG ACTATATCAG AGTCCTTGAC TTTGATGTTA AGCGCAAATA TTTCCGACAG
    GAGTTGGAGC GACTGGATGA AGGGCTAAGA AAAGAGGATA TGGCAGTGCA TGTGCGGCGA
    GATCATGTGT TTGAGGATTC CTACCGGGAG CTGCACCGCA AATCCCCTGA AGAAATGAAG
    AATAGATTGT ACATAGTATT TGAAGGAGAA GAAGGGCAGG ATGCAGGTGG GCTGCTTCGG
    GAGTGGTACA TGATTATCTC CAGGGAGATG TTCAATCCCA TGTATGCTTT GTTCCGCACT
    TCCCCAGGTG ACCGCGTCAC CTACACTATT AATCCTTCCT CCCACTGTAA CCCTAACCAC
    CTCAGCTACT TCAAGTTTGT GGGCCGGATA GTTGCCAAAG CGGTTTATGA CAACCGGCTA
    CTGGAGTGTT ACTTCACTAG GTCCTTCTAC AAGCATATAT TGGGCAAGTC TGTCAGATAC
    ACAGACATGG AGAGTGAAGA TTATCACTTC TATCAGGGTC TGGTGTACTT GCTTGAAAAT
    GATGTCTCCA CACTGGGATA TGACTTGACT TTCAGCACTG AGGTTCAAGA ATTTGGTGTC
    TGTGAAGTCC GAGACCTAAA ACCAAACGGT GCAAACATTC TGGTAACAGA AGAAAACAAA
    AAGGAGTATG TTCATCTTGT GTGCCAGATG AAGATGACAG GTGCAATACG TAAGCAATTG
    GCTGCCTTCC TTGAGGGATT TTATGAGATA ATCCCCAAAC GTCTGATCTC CATCTTCACT
    GAGCAGGAAT TGGAACTGCT TATCTCTGGC CTTCCCACCA TAGATATTGA TGACCTAAAA
    TCAAACACTG AGTATCACAA ATACCAGTCT AACTCCATCC AGATTCAGTG GTTCTGGAGA
    GCGTTGCGCT CATTCGATCA AGCTGACCGG GCAAAGTTCC TTCAGTTTGT TACTGGAACT
    TCAAAGGTCC CATTACAGGG TTTTGCTGCG CTGGAAGGAA TGAACGGCAT CCAGAAATTC
    CAAATCCACA GAGATGATAG ATCTACAGAC AGGCTGCCTT CTGCGCACAC GTGCTTCAAC
    CAGTTGGACC TTCCAGCCTA TGAGAGTTAC GAGAAGCTAC GTCACATGCT GCTGTTAGCC
    ATCCAGGAGT GCTCTGAGGG CTTTGGCCTT GCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65593
    Clone ID Related Accession (Same CDS sequence) XM_031892173.1
    Accession Version XM_031892173.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
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    1801
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    1981
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    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGGCGAT ACAGCCTCTA ACCTGGGCAG TGCTGTTGAT
    GAACTGATGA GGCACCAGCC CACGTTAAAA ACAGACGCTA CAACTGCCAT CATTAAGCTC
    TTAGAAGAGA TATGTAACCT TGGTAGGGAC CCAAAATACA TCTGTCAGAA GCCATCTATT
    CAGAAAGCTG ATGGCACGGC TACTGCTCCT CCACCACGTT CCAACCATGC AGCAGAGGAG
    GCTTCTAGTG AGGATGAAGA GGAGGAGGAG GTACAAGCAA TGCAGAGTTT CAGCACAAAT
    CAGCAAAGTG AAGTTGAACC CAGCCAGCAT GTTGTTGGCA CAGAGGAGAG GATTCCTATT
    CCCCTAATGG ATTATATTCT CAATGTGATG AAATTTGTGG AATCAATTCT AAGTAACAAC
    ACAACTGATG ATCACTGCCA GGAATTTGTC AGTCAGAAGG GGCTCCTTCC ACTTGTCACC
    ATCCTTGGGC TTCCCAACCT ACCTATTGAC TTTCCTACCT CTGCTGCTTG CCAAGCTGTG
    GCAGGTGTCT GCAAATCCAT TTTGACTCTT TCTCATGAGC CAAAAGTTCT CCAAGAAGGA
    CTTCTTCAGC TGGATTCAAT TCTTTCATCT CTTGATCCAC TTCATCGGCC CATTGAGGCT
    CCTGGTGGCT CAGTACTGCT TCGAGAGTTG GCATGTGCTG GAAATGTGGC AGATGCTACT
    TTGTCTGCTC AAGCAACTCC ATTATTACAC GCTCTCACTG CAGCCCATGC CTACATCATG
    ATGTTTGTGC ATACATGCAG GGTTGGACAG AGTGAAATCC GTGCCATCTC TGTGAACCAG
    TGGGGATCAC AGCTGGGTCT CAATGTTTTG AACAAGTTGA GCCAGCTCTA CTGCTCCCTT
    GTATGGGAAA GTACAGTTTT GCTGTCACTT TGTACTCCTA ACAGTTTGCC TCCTGGGTGT
    GAGTTTGGCC AAGCAGATAT GCAGAAATTA GCCCCAAAGG AGGAAAAGGC AACCACTGTA
    TTGCCCCAAG GAACCAAAAG ACCTGAAAGT GAGGTTGAAG GCACAGTTGT CAGTGAGGAA
    GCTTCAGGCT TACTTGAAGG AATTGGACTG GATGGAGATA CTTTAGCTCC AATGGAAACT
    GATGAACCCA GCACTTCTGA TGCAAAGGGC AAACCGAAAA TAACCCCAGC TATGGCGGCC
    AGAATCAAGC AAATCAAACC ATTACTATCA GCTTCTTCTA GGTTGGGCCG GGCTTTGGCA
    GAGCTGTTTG GGTTGCTTGT CAAACTGTGT GTAGGTTCCC CTGTTCGGCA AAGGAGAAGT
    CACCATGCTG CCAGCACTAC CACAGCCCCT ACACCAGCTG CTCGCTCCAC AGCCTCTGCC
    CTCACCAAAC TGCTCACAAA AGGGCTGTCT TGGCAGCCTC CACCCTATAC CCCTACTCCT
    AGGTTCAGAT TGACTTTCTT TATCTGCTCC GTTGGGTTCA CATCCCCCAT GCTGTTTGAT
    GAAAGGAAAT ACCCATATCA CCTCATGCTG CAGAAATTCC TTTGCTCTGG TGGGCACAAT
    GCACTCTTTG AGACTTTTAA TTGGGCACTT TCAATGGGTG GAAGAGTTCC TGTTGCTGAG
    GGCCTGGAAC ACAGTGATCT TCCTGATGGT ACTGGAGAGT TTCTAGATGC TTGGCTTATG
    TTAGTGGAAA AGATGGTGAA TCCTACCACT GTGCTGGAAT CTCCTCATTC ACTTCCAACT
    ACAAAGTTAC CTGGAGGGCA GAATAATCCA CAGTTCAGTG CTTTACGCTT CCTTGTAGTC
    ACACAAAAGG CAGCTTTTAC CTGTATCAAA AACCTTTGGA ATCGGAAACC CCTGAAAGTA
    TATGGAGGGC GTATGGCAGA ATCAATGCTT GCAATCTTAT GTCATATACT TCGCGGTGAG
    CCAGTTATCC GTGAGCGTTT GAGCAAGGAG CGAGAGGGGA GCCGTGAAGA GGAGGGGCAG
    CAGGATGAGA GTGGAGCAAG ACGGGAGCCT CAAGTGAACC AACAGCAACT TCAGCAGCTG
    ATGGATATGG GTTTCACAAG GGAGCATGCT CTGGAAGCTC TGCTTAACAC GAGTACTATG
    GAGCAGGCCA CTGAATATCT CCTCACCCAC CCTCCACCTC TTATTGGCGG AGTTGTACGG
    GACTTAAGTA TGTCAGAGGA AGATCAGATG CTGAGAGCCA TTGCCATGTC GCTGGGACAA
    GATATCCCTA TGGAACAGAG GGCAGAACTG CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG
    GAGGAACGCA AGGCACGAGA AAAACAAGAG GAAGAGGAAG CTAAATGCTT GGAAAAGTTT
    CAAGATGCAG AACCCCTGGA ACAGGATGAG CTTCAAGGAT TCACAGACAC CATGCTCCCT
    GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA GACACAGTCT ATAGAGTGTG TGATCTTATA
    ATGACTGCCA TAAAGCGCAA TGGGGCAGAC TATCGAGACA TGATTCTAAA ACAAGTGGTT
    AACCAGGTAT GGGAAGCCGC TGATGTTCTT ATTAAAGCTG CACTGCCGCT GACCACAAGT
    GACACAAAAA CTGTGTCTGA GTGGATCAGC CAAATGGCTA CTCTTCCTCA AGCTTCTAAC
    TTGGCCACTC GCATCCTTTT GCTCACACTG CTGTTTGAGG AGCTAAAACT GCCATGTGCT
    AGAGTTGTTG AAACAAGTGG AATACTTAAC GTACTAATTA AGCTGCTTGA AGTGGTTCAG
    CCATGTTTGC AGGCAGCCAA GGAGCAAAAG GAAGTTCAGA CACCAAAGTG GATTACACCA
    GTCCTCCTCT TAATAGATTT TTATGAGAAG ACCTCAATTT CTTCTAAAAG AAGAATGCAA
    ATGAACAGGT ACCTTCAAGC TAATGGAAAC AACTGGCGAT GGTTTGATGA TCGGTCTGGC
    CGTTGGTGCA GCTACAGTGC TAGTAACAAC AGCACTATTG ATGCAGCCTG GAAAGCAGGA
    GAAACATGTG TGCGATTTAC AGCTGGCCGT CGAAGATATA CTGTGCAGTT TACCACAATG
    GTTCAGGTAA ATGAAGAGAC CGGCAATAGA CGACCTGTCA TGCTGACTCT CTTGCGTGTT
    CCGAGACTTT TAAAGAGTGG AAAAAATGGA AATGGACAGG AACTTGAAAA GACACTTGAG
    GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA GACGAAAAAG TGCCTGAGCC CATTCCAGTT
    CCTGAAACCT CTTCCACTGA GAAAGATGCA CCAACTGAAG AGCCCAAAGT TGGAGACATA
    GTAATTCAGG GTCTGGCTGA AGAAATGGTG ACCGTCCTTA TACGTTCCTG CGTCGGCATG
    CTTGGTGTTC CTGTTGATCC AGATACTCTT CATGCAACCT TGCGTCTTTG CCTGCGTCTC
    ACACGTGACC ACAAATATGC CATGATGTTT GCAGAGCTTA AGAGCACTCG AATGATCCTC
    AGCTTAACTC AAAGCTCTGG ATTCAATGGC TTTACTCCCC TTGTGACGCT CCTATTTAGG
    CACATCATAG AGGATCCTTG CACCCTCCAG CACACCATGG AAAAGGTTGT ACGGTCTGCA
    GCAACTAGTG GGGCAGGGAG TACCACATCT GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT
    GAAATCAACT ATATACTTAG AGTACTGGGT CCAGCAGCTT GTCGAAACCC TGACATTTTC
    ACTGAAGTGG CAAACAGTTG CATTCGCATT GCTCTCCCTG CACCTCGTGG ATCTGGAACT
    GCCTCTGATG ATGAGTTTGA GAACCTTCGG ATCAAAGGTC CTAATGCAGT TCAGTTGGTT
    AAAACCACCC CTATAAAGCC CTCCACCTTG CCAGTCATCC CTGATACAAT TAAGGAAGTA
    ATCTATGACA TGCTGAATGC ACTGGCAGCT TACCACGCCC CTGAGGAAGT ATCTGAAAAG
    TCTGAGCAAA AACCTGTTGG CATGAACCAG GAAGTCAGCC AGATCCTACA GGACATGGGT
    GATGAGGTTT TCCAGCAATA TCGGGACTTA ACCAGACAGG GAAGCGACTT TGATGGTCAG
    ACCAGTTTTG CCATCAACGC GCGAGTCTTT ACTGCTGACA GCACAGCTGC CGAAACACCT
    CAGTCTGGTA CACCTCAAGG TGAAACTGCC TTAACACCTG AAGAAAACCG AGAAGGGTTA
    AAGAAAGATA AGGAAGGAGA CCGTAGTTCA GAAGATGGAA AGCAGAAGGC AAAAGGTAGT
    AAGCCTTTGC TGCCTACCTC TTCCATTCTT CGACTACTTG CTGAACTTGT CAGGTCCTAT
    GTCGGAATAG CCTCGCTGAT CGCCACCTAC AATTACACTG CAGGACAATC TGAACTAATT
    AAAGAGGATT GCAGTGTATT GGCGTTTGTG CTGGATCACC TGCTACCCCA CACACATAAT
    GCAGAGGACA AGGACACACC AGCCCTAGCC CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA
    GGAAGTGGCA CTGATGCCCA GGTTGCACTA GTGAATGAAG TAAAGGCAGC TCTTGGCAGA
    GCCCTAGCAA TGGCTGAGAG CACGGAGAAG CATGCTAGGT TGCAGGCTGT GATGTGCATT
    ATCAGTACCA TTATGGAGTC ATGTCCATCC ACCTCCAGCT TCTACAGCAC AGCAACCACC
    AAAACCCAGC ACAATGGCAT GAACAACATC ATCAGACTCT TTTTGAAAAA GGGGCTGGTT
    AGTGATTTAG CCAGGGTCCC TCACAGCCTT GATTTATCCA GTCCAAATAT GGCAAACACC
    GTGAATGCAG CACTGAAGCC ACTCGAGACA CTGTCTCGCA TTGTCAATCA ACCCAGCAGT
    TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG AATAAATCTG AGGCAGATAC ACATGGAACA
    GCCCAGGATC CCAACAGTAA TCAGCCAGAT ACAGGTGAAA CAGGAACAGA AGTGCAGGAA
    GAGGACCCAG ATGCTTCTCA TGCTGAAGTA GCTGATGGAG ACATCATGGA TGGAGAGACA
    GAGACTGATG CTGTGGTTAT TGCTGGCCAG CCTGAAGTCC TTACTACACA AGAAATGCAG
    GTAGAGAATG AGCTTGAAGA TCTGATAGAT GAACTTCTTG AAAGGGATGG TGCTGCAGGT
    AACCGGGTCG GAGAAGATGA ATCACAGGAA GATGTGCTGA TGGATGAGCC TCCTACTAAC
    CTCAGCCAAG CCTCCACTCT TCAGGCTAAT CGAGAAGACT CTATGAACAT CTTAGACCCA
    GAGGATGAGG AAGAGCACAC CCAAGAAGAG GACAGCAGTG GCAGCAATGA AGATGAAGAT
    GACAGCCAGG AAGAGGAGGA GGAAGAGGAA GATGAGGACG ATCAGGATGA TGAGGAGGGT
    GAGGAAGGGG ATGAGGATGA TGATGATGAC TGTTCAGAGA TGGAACTGGA TGAGGATTAT
    CCAGACATGA ATGCCTCACC TCTTGTACGC TTTGAACGTT TTGACAGAGA AGATGATTTA
    ATCATAGAGT TTGATAACAT GTTCTCCAGT GCCACTGAGA TCCCTCCATC CCCTGGTAAC
    ATCCCTACTA CACACCCCCT AATGGTGCGA CACGCTGATC ACGGCTCCTT GACTCTTGGC
    AGTGGCACTT CCACTACTCG CCTGGCACAG GGCATCGGGC GCAGCCAGAG AACACTCCGA
    CAACTCACAG CCAACACCGG TCACACAATA CATGTTCATT ATCCTGGCAA CAGGCAGCCA
    AATCCACCTT TAATCCTCCA GAGGTTGCTG GGGCCCTCAG CCGCTGCTGA CATCTTGCAA
    CTAAGCAGCA GTCTTCCTCT GCAGAGTCGT GGCCGAGCAC GTCTACTTGT TGGCAACGAT
    GATGTCCACA TCATAGCACG CTCTGATGAT GAGATGCTTG ATGATTTTTT CCATGATCAG
    AGTACAGCCA CAAGTCAGGC TGGCACACTT TCCAGCATTC CAACTGCGCT GACCCGCTGG
    ACAGAAGAGT GTAAGGTCTT GGATGCTGAA AGCATGCATG ACTGTGTATC TGTGGTCAAA
    GTCCCAATCA TCAATCATTT GGAATCTTTG CGTGATGAGG AGCTTGAGGA GCGCAGGGAA
    AAAAGAAGGA AACAACTAGC AGAAGAGGAG GCCAAGATGG CAGAGAAATC CAAGGAGGAG
    AAGGAAGCAA AGGAGCAAAA TTCACAGGGT GCCAATTCAA AGCAGAATAC CCCATTTGAA
    CTGAGCCTTG CAGATCAAGA TGGAAGTGGT CGTCCTGAAA ATTTCCCAAC TGCACCATCT
    TCTGCAGAAG GAACTCCAAC AAGTCCTGAA ATTGTTCGTT TAGATCCCAC CCTAAGGGCT
    ACTGAGGCTA CTCTGGCAGA GCTGACTCAG GGTCTGCCTG CTGATACAGG CACGGAGGAT
    GTTGGGCAAC AACTCATGCC ATCTCACCAA GAGGACTCTG GACCAGGACG GCCAAGTGTA
    GAGGGGGATG CCACACAAAT GGAGCTATCG CCAGCACCCA CTATAACCTC CTTATCACCA
    GAGCGAGCAG AGGATTCAGA TGCTTTGACA GCTGTTAGTA GCCAACTAGA GGGCTCGCCT
    ATGGACACCA GCAGTCTTGC CTCTGGGCCC TTAGAGGATG GCGTAGGGGA GGCACCTGGA
    ATTGTTAATC CAGAGCAGCC TCTTGTGGTT GGCAGTAGCA TTGCTGTTGG ACAGCAACCA
    GTGGAACCAC CGACGACCTG TGAAACCCAA CCAGTAGTTT CTCAACACAG TGATAGCTCA
    CAAATTGCCC CTTCCTCTGA GTTGCCTTCC ACAAGAGAAA GTGCTGTGAC CTTGTTGGCT
    ACAGATTCTA GTGGTATCCT TGAAGAACCT TTGCCTTCTA CAAGCAGTGA AGAGGAAGAC
    CCATTAGCAG GCATAAATCT TCCAGAGGGT GTGGATCCTT CTTTCCTAGC GGCTCTTCCT
    GAAGACATCC GCAGAGAGGT GCTGCAGAAT CAGTTGGGCA TACGGCCGCC ATCGCGACCT
    CCACCCACTG CTGCCCCAGT TATTCCTCCT CCTGTGGTTG CTAATGCTGG TGTCACGGAG
    GTCAGCCCTG AGTTCTTGGC AGCGTTGCCA CCAGCCATTC AGGAAGAGGT CCTAGCACAG
    CAGCGAGCAG AACAGCAGAG GCGGGAACTT GCTCAGACCA CTACGTCTGA TACACCCATG
    GATCCTGTGA CTTTCATTCA GACGTTACCT TCTGATCTAC GTCGTAGTGT GTTAGAAGAC
    ATGGAGGACA GTGTTTTAGC TGTGATGCCA CCTGACATTG CTGCAGAGGC ACAGGCTCTA
    AGGCGAGAGC AAGAAGCTCG GCAGAGGCAA CTAATGCATG AACGACTGTT CGGGCACAGT
    AGCACATCTG CACTCTCTGC CATCCTTAGG AGTCCTGCTT TCACGAGTCG CTTGAGTGGA
    AACCGAGGTG TTCAGTACAC ACGGTTGGCT GTACAGAGAG GGAACACTTT CCAAATGGGA
    AGCAGCAGTA CACATACCAG ACCAACCGGT AGTAATGTTG ACAGCCTACT ACGATTACGA
    GGCCGACTAT TACTTGATCA TGAAGCCCTC TCCTGTCTGC TTGTCCTACT GTTTGTTGAT
    GAACCCAAGC TAAACACCAG TCGCTTGCAC CGTGTTCTAC GCAACCTCTG CTACCATGCA
    CAAACAAGGA ACTGGGTGAT TAGAAGCTTG TTGTCTATAC TGCAGCGTAG CAGTGAAAGT
    GAGCTTTGTA TAGAAAGCCC AAAGACTTCT ATTGGGGGCA CTGATAAACG GGGTGCAAAA
    ACAAGTCATG ACAACCGCCC TTTAGAATTT CTGCATAAGA TGGAATCGAA GAATTCCAAC
    CAGCTCTCCT GGCTTTCTGT ATCCATGGAC GCTGCTCTTG GCTGCCGTAC GAACATCTTC
    CAAATCCAGA GGTCCCTAGG ACGCAAGCAT ACAGAAAAGC ATTCAGCCTG TGGTTCTACT
    GTCCACATCC ATCCCCAAGC AGCCCCTGTA GTGTGTAGAC ATGTGCTTGA CACACTAATA
    CAGCTAGCTA AGGTGTTTCC CAGTCATTTC ACACAGCAGC GAACCAAAGA ATGTAGTGTG
    GAAAACAGTG ACAGCAGGGA GCGGAGTATA AAACTTACTT GTAGTCCGTG TCTTGCACCA
    TCTAGTAGTA TTGGAGCAAG CAACATCTCC ACAGATTTCT GGGACTTGCT AGTCAAGCTA
    GACAACATGA ATGTGAGCCG GAAGGGGAAA ACGTCTATAA AATCTGTGCC AGTGAGCTCT
    GGTTCTGAAG GCGAGAGCAA TCAGTGCAGC CTGGAGGCCT CCCCCTTAGG ACAGCTTATG
    AATATGCTGT CTCATTCTGT TATTCGCCGC AGTTCGCTGC TTACTGAAAA GCTGCTGAGA
    CTTCTCTCCC TTATCTCTAT TGCCCTTCCT GATAATAAGG CTTCTGAAAC AGCAGGCAAT
    CAGAGCAATT CTATTACCCC AGCACCTGCA CCTACGCCTG CAACTGCACC AGCCCCTGTG
    CCTGTACCTG CTGTAACAGT TCCAGTTGTT GTGAGCACAA ACATAGTGAC AACTGTTACA
    ACCAAAAGTG GAAAATCTCC TGCAAAATCA AGTGAAGTCA GCAATGCACC AGATAAGATG
    GCAGCTTCTG GCCTTACAGA GAAACAGCTG CAGTTGTCTG TTGAGGTTCT GACATCACAC
    TCGTGTTCAG AAGAAGGACT GGAGGATGCC GCCAATGTTC TACTTCAGCT TTCCAGAGGC
    GACCCCAGTA CACGGGACAC TGTACTGAAA CTTCTGCTTA GCGGAGCACA TCACTTGGGG
    TTTACTCTCT GCAAACAGAT AGGTACACTA CTAGCAGAGC TCCGGGAATA TAACCTAGAG
    CAGCAGCGTC GTGCACAGTG TGAAGTTCTT TCTCCAGATG GTATGCCTGA GGAGCAACCC
    CACAGCACAA AGCCAAAAGG CAAAATGCAG AGCAGTGTTA GGTCTCTCCC CACCCCACCT
    ATGCGATCCA GGTTCGACAT TGCAGAGAAT GTGGTGATTG TGGCTTCCCA AAAGCGTCCA
    CTGGGAGGAA GAGAGCTGCA GCTCCCCTCA ATGTCTATGC TGACGTCAAA GACTTCCACA
    CAAAAATTCT TTCTTCGAGT GCTGCAGGTT ATCATCCAGC TTCGCGATGA CACTCGCAGA
    GCCAACAAGA AGGCCAAGCA GACCAGCAGA TTAGGCACTG CCAGTCTGGC CTCGGCTAGC
    AGCATCCAAG CAGCTGTGCA GCAGCTGGAG GCTGAGGCTG ATGCCATTAT ACAAATGGTA
    CGTGAGGGTC AAAGGGCGCG GAGACAGCTG CAAACTGCAA CGGCGGAGGG TTCTGCTCGC
    AGGGAGGAGC AGCCTATGGA TGTTGACCAG CCTTCGCCTG CTACACAGGA GCCTCAGTCA
    GTTGCTTCTG AAGGTTCTCA GGCTGCTGAA AAAAAAGAAG AGGAGAGGTT ACCAGAGTTA
    CCGCTGTTAA GTGAGCAGCT AAGTTTAGAT GAGCTGTGGG ACATGTTGGG AGAGTGCCTG
    AAGGAACTTG AGGAATCTCA TGACCAGCAT GCCGTATTAG TGTTACAGCC AGCCGTAGAA
    GCCTTTTTCC TTGTTCATGC AACAGAGCGG GAGAGCAAGC CTCCTGTGCG GGACACTCGG
    GAAAGCCAGT TATCTCACAT TAAGGATGAA CCACCTCCCC TGTCTCCTGC ACCCCTCACT
    CCAGCCACAC CTTCTTCCTT GGATCCTTTC TTCTCTCGTG AACCTTCATC TATGCACATT
    TCCTCTAGTT TGCCTCCTGA CACTCAGAAG TTCCTAAGAT TTGCTGAGAC TCATCGGACT
    GTATTAAATC AGATCCTTCG CCAGTCCACC ACACACCTAG CTGATGGGCC CTTTGCTGTG
    CTTGTAGACT ATATCAGAGT CCTTGACTTT GATGTTAAGC GCAAATATTT CCGACAGGAG
    TTGGAGCGAC TGGATGAAGG GCTAAGAAAA GAGGATATGG CAGTGCATGT GCGGCGAGAT
    CATGTGTTTG AGGATTCCTA CCGGGAGCTG CACCGCAAAT CCCCTGAAGA AATGAAGAAT
    AGATTGTACA TAGTATTTGA AGGAGAAGAA GGGCAGGATG CAGGTGGGCT GCTTCGGGAG
    TGGTACATGA TTATCTCCAG GGAGATGTTC AATCCCATGT ATGCTTTGTT CCGCACTTCC
    CCAGGTGACC GCGTCACCTA CACTATTAAT CCTTCCTCCC ACTGTAACCC TAACCACCTC
    AGCTACTTCA AGTTTGTGGG CCGGATAGTT GCCAAAGCGG TTTATGACAA CCGGCTACTG
    GAGTGTTACT TCACTAGGTC CTTCTACAAG CATATATTGG GCAAGTCTGT CAGATACACA
    GACATGGAGA GTGAAGATTA TCACTTCTAT CAGGGTCTGG TGTACTTGCT TGAAAATGAT
    GTCTCCACAC TGGGATATGA CTTGACTTTC AGCACTGAGG TTCAAGAATT TGGTGTCTGT
    GAAGTCCGAG ACCTAAAACC AAACGGTGCA AACATTCTGG TAACAGAAGA AAACAAAAAG
    GAGTATGTTC ATCTTGTGTG CCAGATGAAG ATGACAGGTG CAATACGTAA GCAATTGGCT
    GCCTTCCTTG AGGGATTTTA TGAGATAATC CCCAAACGTC TGATCTCCAT CTTCACTGAG
    CAGGAATTGG AACTGCTTAT CTCTGGCCTT CCCACCATAG ATATTGATGA CCTAAAATCA
    AACACTGAGT ATCACAAATA CCAGTCTAAC TCCATCCAGA TTCAGTGGTT CTGGAGAGCG
    TTGCGCTCAT TCGATCAAGC TGACCGGGCA AAGTTCCTTC AGTTTGTTAC TGGAACTTCA
    AAGGTCCCAT TACAGGGTTT TGCTGCGCTG GAAGGAATGA ACGGCATCCA GAAATTCCAA
    ATCCACAGAG ATGATAGATC TACAGACAGG CTGCCTTCTG CGCACACGTG CTTCAACCAG
    TTGGACCTTC CAGCCTATGA GAGTTACGAG AAGCTACGTC ACATGCTGCT GTTAGCCATC
    CAGGAGTGCT CTGAGGGCTT TGGCCTTGCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748033.1
    CDS671..13783
    Translation

    Target ORF information:

    RefSeq Version XM_031892173.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892173.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGGCGAT ACAGCCTCTA ACCTGGGCAG TGCTGTTGAT
    GAACTGATGA GGCACCAGCC CACGTTAAAA ACAGACGCTA CAACTGCCAT CATTAAGCTC
    TTAGAAGAGA TATGTAACCT TGGTAGGGAC CCAAAATACA TCTGTCAGAA GCCATCTATT
    CAGAAAGCTG ATGGCACGGC TACTGCTCCT CCACCACGTT CCAACCATGC AGCAGAGGAG
    GCTTCTAGTG AGGATGAAGA GGAGGAGGAG GTACAAGCAA TGCAGAGTTT CAGCACAAAT
    CAGCAAAGTG AAGTTGAACC CAGCCAGCAT GTTGTTGGCA CAGAGGAGAG GATTCCTATT
    CCCCTAATGG ATTATATTCT CAATGTGATG AAATTTGTGG AATCAATTCT AAGTAACAAC
    ACAACTGATG ATCACTGCCA GGAATTTGTC AGTCAGAAGG GGCTCCTTCC ACTTGTCACC
    ATCCTTGGGC TTCCCAACCT ACCTATTGAC TTTCCTACCT CTGCTGCTTG CCAAGCTGTG
    GCAGGTGTCT GCAAATCCAT TTTGACTCTT TCTCATGAGC CAAAAGTTCT CCAAGAAGGA
    CTTCTTCAGC TGGATTCAAT TCTTTCATCT CTTGATCCAC TTCATCGGCC CATTGAGGCT
    CCTGGTGGCT CAGTACTGCT TCGAGAGTTG GCATGTGCTG GAAATGTGGC AGATGCTACT
    TTGTCTGCTC AAGCAACTCC ATTATTACAC GCTCTCACTG CAGCCCATGC CTACATCATG
    ATGTTTGTGC ATACATGCAG GGTTGGACAG AGTGAAATCC GTGCCATCTC TGTGAACCAG
    TGGGGATCAC AGCTGGGTCT CAATGTTTTG AACAAGTTGA GCCAGCTCTA CTGCTCCCTT
    GTATGGGAAA GTACAGTTTT GCTGTCACTT TGTACTCCTA ACAGTTTGCC TCCTGGGTGT
    GAGTTTGGCC AAGCAGATAT GCAGAAATTA GCCCCAAAGG AGGAAAAGGC AACCACTGTA
    TTGCCCCAAG GAACCAAAAG ACCTGAAAGT GAGGTTGAAG GCACAGTTGT CAGTGAGGAA
    GCTTCAGGCT TACTTGAAGG AATTGGACTG GATGGAGATA CTTTAGCTCC AATGGAAACT
    GATGAACCCA GCACTTCTGA TGCAAAGGGC AAACCGAAAA TAACCCCAGC TATGGCGGCC
    AGAATCAAGC AAATCAAACC ATTACTATCA GCTTCTTCTA GGTTGGGCCG GGCTTTGGCA
    GAGCTGTTTG GGTTGCTTGT CAAACTGTGT GTAGGTTCCC CTGTTCGGCA AAGGAGAAGT
    CACCATGCTG CCAGCACTAC CACAGCCCCT ACACCAGCTG CTCGCTCCAC AGCCTCTGCC
    CTCACCAAAC TGCTCACAAA AGGGCTGTCT TGGCAGCCTC CACCCTATAC CCCTACTCCT
    AGGTTCAGAT TGACTTTCTT TATCTGCTCC GTTGGGTTCA CATCCCCCAT GCTGTTTGAT
    GAAAGGAAAT ACCCATATCA CCTCATGCTG CAGAAATTCC TTTGCTCTGG TGGGCACAAT
    GCACTCTTTG AGACTTTTAA TTGGGCACTT TCAATGGGTG GAAGAGTTCC TGTTGCTGAG
    GGCCTGGAAC ACAGTGATCT TCCTGATGGT ACTGGAGAGT TTCTAGATGC TTGGCTTATG
    TTAGTGGAAA AGATGGTGAA TCCTACCACT GTGCTGGAAT CTCCTCATTC ACTTCCAACT
    ACAAAGTTAC CTGGAGGGCA GAATAATCCA CAGTTCAGTG CTTTACGCTT CCTTGTAGTC
    ACACAAAAGG CAGCTTTTAC CTGTATCAAA AACCTTTGGA ATCGGAAACC CCTGAAAGTA
    TATGGAGGGC GTATGGCAGA ATCAATGCTT GCAATCTTAT GTCATATACT TCGCGGTGAG
    CCAGTTATCC GTGAGCGTTT GAGCAAGGAG CGAGAGGGGA GCCGTGAAGA GGAGGGGCAG
    CAGGATGAGA GTGGAGCAAG ACGGGAGCCT CAAGTGAACC AACAGCAACT TCAGCAGCTG
    ATGGATATGG GTTTCACAAG GGAGCATGCT CTGGAAGCTC TGCTTAACAC GAGTACTATG
    GAGCAGGCCA CTGAATATCT CCTCACCCAC CCTCCACCTC TTATTGGCGG AGTTGTACGG
    GACTTAAGTA TGTCAGAGGA AGATCAGATG CTGAGAGCCA TTGCCATGTC GCTGGGACAA
    GATATCCCTA TGGAACAGAG GGCAGAACTG CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG
    GAGGAACGCA AGGCACGAGA AAAACAAGAG GAAGAGGAAG CTAAATGCTT GGAAAAGTTT
    CAAGATGCAG AACCCCTGGA ACAGGATGAG CTTCAAGGAT TCACAGACAC CATGCTCCCT
    GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA GACACAGTCT ATAGAGTGTG TGATCTTATA
    ATGACTGCCA TAAAGCGCAA TGGGGCAGAC TATCGAGACA TGATTCTAAA ACAAGTGGTT
    AACCAGGTAT GGGAAGCCGC TGATGTTCTT ATTAAAGCTG CACTGCCGCT GACCACAAGT
    GACACAAAAA CTGTGTCTGA GTGGATCAGC CAAATGGCTA CTCTTCCTCA AGCTTCTAAC
    TTGGCCACTC GCATCCTTTT GCTCACACTG CTGTTTGAGG AGCTAAAACT GCCATGTGCT
    AGAGTTGTTG AAACAAGTGG AATACTTAAC GTACTAATTA AGCTGCTTGA AGTGGTTCAG
    CCATGTTTGC AGGCAGCCAA GGAGCAAAAG GAAGTTCAGA CACCAAAGTG GATTACACCA
    GTCCTCCTCT TAATAGATTT TTATGAGAAG ACCTCAATTT CTTCTAAAAG AAGAATGCAA
    ATGAACAGGT ACCTTCAAGC TAATGGAAAC AACTGGCGAT GGTTTGATGA TCGGTCTGGC
    CGTTGGTGCA GCTACAGTGC TAGTAACAAC AGCACTATTG ATGCAGCCTG GAAAGCAGGA
    GAAACATGTG TGCGATTTAC AGCTGGCCGT CGAAGATATA CTGTGCAGTT TACCACAATG
    GTTCAGGTAA ATGAAGAGAC CGGCAATAGA CGACCTGTCA TGCTGACTCT CTTGCGTGTT
    CCGAGACTTT TAAAGAGTGG AAAAAATGGA AATGGACAGG AACTTGAAAA GACACTTGAG
    GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA GACGAAAAAG TGCCTGAGCC CATTCCAGTT
    CCTGAAACCT CTTCCACTGA GAAAGATGCA CCAACTGAAG AGCCCAAAGT TGGAGACATA
    GTAATTCAGG GTCTGGCTGA AGAAATGGTG ACCGTCCTTA TACGTTCCTG CGTCGGCATG
    CTTGGTGTTC CTGTTGATCC AGATACTCTT CATGCAACCT TGCGTCTTTG CCTGCGTCTC
    ACACGTGACC ACAAATATGC CATGATGTTT GCAGAGCTTA AGAGCACTCG AATGATCCTC
    AGCTTAACTC AAAGCTCTGG ATTCAATGGC TTTACTCCCC TTGTGACGCT CCTATTTAGG
    CACATCATAG AGGATCCTTG CACCCTCCAG CACACCATGG AAAAGGTTGT ACGGTCTGCA
    GCAACTAGTG GGGCAGGGAG TACCACATCT GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT
    GAAATCAACT ATATACTTAG AGTACTGGGT CCAGCAGCTT GTCGAAACCC TGACATTTTC
    ACTGAAGTGG CAAACAGTTG CATTCGCATT GCTCTCCCTG CACCTCGTGG ATCTGGAACT
    GCCTCTGATG ATGAGTTTGA GAACCTTCGG ATCAAAGGTC CTAATGCAGT TCAGTTGGTT
    AAAACCACCC CTATAAAGCC CTCCACCTTG CCAGTCATCC CTGATACAAT TAAGGAAGTA
    ATCTATGACA TGCTGAATGC ACTGGCAGCT TACCACGCCC CTGAGGAAGT ATCTGAAAAG
    TCTGAGCAAA AACCTGTTGG CATGAACCAG GAAGTCAGCC AGATCCTACA GGACATGGGT
    GATGAGGTTT TCCAGCAATA TCGGGACTTA ACCAGACAGG GAAGCGACTT TGATGGTCAG
    ACCAGTTTTG CCATCAACGC GCGAGTCTTT ACTGCTGACA GCACAGCTGC CGAAACACCT
    CAGTCTGGTA CACCTCAAGG TGAAACTGCC TTAACACCTG AAGAAAACCG AGAAGGGTTA
    AAGAAAGATA AGGAAGGAGA CCGTAGTTCA GAAGATGGAA AGCAGAAGGC AAAAGGTAGT
    AAGCCTTTGC TGCCTACCTC TTCCATTCTT CGACTACTTG CTGAACTTGT CAGGTCCTAT
    GTCGGAATAG CCTCGCTGAT CGCCACCTAC AATTACACTG CAGGACAATC TGAACTAATT
    AAAGAGGATT GCAGTGTATT GGCGTTTGTG CTGGATCACC TGCTACCCCA CACACATAAT
    GCAGAGGACA AGGACACACC AGCCCTAGCC CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA
    GGAAGTGGCA CTGATGCCCA GGTTGCACTA GTGAATGAAG TAAAGGCAGC TCTTGGCAGA
    GCCCTAGCAA TGGCTGAGAG CACGGAGAAG CATGCTAGGT TGCAGGCTGT GATGTGCATT
    ATCAGTACCA TTATGGAGTC ATGTCCATCC ACCTCCAGCT TCTACAGCAC AGCAACCACC
    AAAACCCAGC ACAATGGCAT GAACAACATC ATCAGACTCT TTTTGAAAAA GGGGCTGGTT
    AGTGATTTAG CCAGGGTCCC TCACAGCCTT GATTTATCCA GTCCAAATAT GGCAAACACC
    GTGAATGCAG CACTGAAGCC ACTCGAGACA CTGTCTCGCA TTGTCAATCA ACCCAGCAGT
    TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG AATAAATCTG AGGCAGATAC ACATGGAACA
    GCCCAGGATC CCAACAGTAA TCAGCCAGAT ACAGGTGAAA CAGGAACAGA AGTGCAGGAA
    GAGGACCCAG ATGCTTCTCA TGCTGAAGTA GCTGATGGAG ACATCATGGA TGGAGAGACA
    GAGACTGATG CTGTGGTTAT TGCTGGCCAG CCTGAAGTCC TTACTACACA AGAAATGCAG
    GTAGAGAATG AGCTTGAAGA TCTGATAGAT GAACTTCTTG AAAGGGATGG TGCTGCAGGT
    AACCGGGTCG GAGAAGATGA ATCACAGGAA GATGTGCTGA TGGATGAGCC TCCTACTAAC
    CTCAGCCAAG CCTCCACTCT TCAGGCTAAT CGAGAAGACT CTATGAACAT CTTAGACCCA
    GAGGATGAGG AAGAGCACAC CCAAGAAGAG GACAGCAGTG GCAGCAATGA AGATGAAGAT
    GACAGCCAGG AAGAGGAGGA GGAAGAGGAA GATGAGGACG ATCAGGATGA TGAGGAGGGT
    GAGGAAGGGG ATGAGGATGA TGATGATGAC TGTTCAGAGA TGGAACTGGA TGAGGATTAT
    CCAGACATGA ATGCCTCACC TCTTGTACGC TTTGAACGTT TTGACAGAGA AGATGATTTA
    ATCATAGAGT TTGATAACAT GTTCTCCAGT GCCACTGAGA TCCCTCCATC CCCTGGTAAC
    ATCCCTACTA CACACCCCCT AATGGTGCGA CACGCTGATC ACGGCTCCTT GACTCTTGGC
    AGTGGCACTT CCACTACTCG CCTGGCACAG GGCATCGGGC GCAGCCAGAG AACACTCCGA
    CAACTCACAG CCAACACCGG TCACACAATA CATGTTCATT ATCCTGGCAA CAGGCAGCCA
    AATCCACCTT TAATCCTCCA GAGGTTGCTG GGGCCCTCAG CCGCTGCTGA CATCTTGCAA
    CTAAGCAGCA GTCTTCCTCT GCAGAGTCGT GGCCGAGCAC GTCTACTTGT TGGCAACGAT
    GATGTCCACA TCATAGCACG CTCTGATGAT GAGATGCTTG ATGATTTTTT CCATGATCAG
    AGTACAGCCA CAAGTCAGGC TGGCACACTT TCCAGCATTC CAACTGCGCT GACCCGCTGG
    ACAGAAGAGT GTAAGGTCTT GGATGCTGAA AGCATGCATG ACTGTGTATC TGTGGTCAAA
    GTCCCAATCA TCAATCATTT GGAATCTTTG CGTGATGAGG AGCTTGAGGA GCGCAGGGAA
    AAAAGAAGGA AACAACTAGC AGAAGAGGAG GCCAAGATGG CAGAGAAATC CAAGGAGGAG
    AAGGAAGCAA AGGAGCAAAA TTCACAGGGT GCCAATTCAA AGCAGAATAC CCCATTTGAA
    CTGAGCCTTG CAGATCAAGA TGGAAGTGGT CGTCCTGAAA ATTTCCCAAC TGCACCATCT
    TCTGCAGAAG GAACTCCAAC AAGTCCTGAA ATTGTTCGTT TAGATCCCAC CCTAAGGGCT
    ACTGAGGCTA CTCTGGCAGA GCTGACTCAG GGTCTGCCTG CTGATACAGG CACGGAGGAT
    GTTGGGCAAC AACTCATGCC ATCTCACCAA GAGGACTCTG GACCAGGACG GCCAAGTGTA
    GAGGGGGATG CCACACAAAT GGAGCTATCG CCAGCACCCA CTATAACCTC CTTATCACCA
    GAGCGAGCAG AGGATTCAGA TGCTTTGACA GCTGTTAGTA GCCAACTAGA GGGCTCGCCT
    ATGGACACCA GCAGTCTTGC CTCTGGGCCC TTAGAGGATG GCGTAGGGGA GGCACCTGGA
    ATTGTTAATC CAGAGCAGCC TCTTGTGGTT GGCAGTAGCA TTGCTGTTGG ACAGCAACCA
    GTGGAACCAC CGACGACCTG TGAAACCCAA CCAGTAGTTT CTCAACACAG TGATAGCTCA
    CAAATTGCCC CTTCCTCTGA GTTGCCTTCC ACAAGAGAAA GTGCTGTGAC CTTGTTGGCT
    ACAGATTCTA GTGGTATCCT TGAAGAACCT TTGCCTTCTA CAAGCAGTGA AGAGGAAGAC
    CCATTAGCAG GCATAAATCT TCCAGAGGGT GTGGATCCTT CTTTCCTAGC GGCTCTTCCT
    GAAGACATCC GCAGAGAGGT GCTGCAGAAT CAGTTGGGCA TACGGCCGCC ATCGCGACCT
    CCACCCACTG CTGCCCCAGT TATTCCTCCT CCTGTGGTTG CTAATGCTGG TGTCACGGAG
    GTCAGCCCTG AGTTCTTGGC AGCGTTGCCA CCAGCCATTC AGGAAGAGGT CCTAGCACAG
    CAGCGAGCAG AACAGCAGAG GCGGGAACTT GCTCAGACCA CTACGTCTGA TACACCCATG
    GATCCTGTGA CTTTCATTCA GACGTTACCT TCTGATCTAC GTCGTAGTGT GTTAGAAGAC
    ATGGAGGACA GTGTTTTAGC TGTGATGCCA CCTGACATTG CTGCAGAGGC ACAGGCTCTA
    AGGCGAGAGC AAGAAGCTCG GCAGAGGCAA CTAATGCATG AACGACTGTT CGGGCACAGT
    AGCACATCTG CACTCTCTGC CATCCTTAGG AGTCCTGCTT TCACGAGTCG CTTGAGTGGA
    AACCGAGGTG TTCAGTACAC ACGGTTGGCT GTACAGAGAG GGAACACTTT CCAAATGGGA
    AGCAGCAGTA CACATACCAG ACCAACCGGT AGTAATGTTG ACAGCCTACT ACGATTACGA
    GGCCGACTAT TACTTGATCA TGAAGCCCTC TCCTGTCTGC TTGTCCTACT GTTTGTTGAT
    GAACCCAAGC TAAACACCAG TCGCTTGCAC CGTGTTCTAC GCAACCTCTG CTACCATGCA
    CAAACAAGGA ACTGGGTGAT TAGAAGCTTG TTGTCTATAC TGCAGCGTAG CAGTGAAAGT
    GAGCTTTGTA TAGAAAGCCC AAAGACTTCT ATTGGGGGCA CTGATAAACG GGGTGCAAAA
    ACAAGTCATG ACAACCGCCC TTTAGAATTT CTGCATAAGA TGGAATCGAA GAATTCCAAC
    CAGCTCTCCT GGCTTTCTGT ATCCATGGAC GCTGCTCTTG GCTGCCGTAC GAACATCTTC
    CAAATCCAGA GGTCCCTAGG ACGCAAGCAT ACAGAAAAGC ATTCAGCCTG TGGTTCTACT
    GTCCACATCC ATCCCCAAGC AGCCCCTGTA GTGTGTAGAC ATGTGCTTGA CACACTAATA
    CAGCTAGCTA AGGTGTTTCC CAGTCATTTC ACACAGCAGC GAACCAAAGA ATGTAGTGTG
    GAAAACAGTG ACAGCAGGGA GCGGAGTATA AAACTTACTT GTAGTCCGTG TCTTGCACCA
    TCTAGTAGTA TTGGAGCAAG CAACATCTCC ACAGATTTCT GGGACTTGCT AGTCAAGCTA
    GACAACATGA ATGTGAGCCG GAAGGGGAAA ACGTCTATAA AATCTGTGCC AGTGAGCTCT
    GGTTCTGAAG GCGAGAGCAA TCAGTGCAGC CTGGAGGCCT CCCCCTTAGG ACAGCTTATG
    AATATGCTGT CTCATTCTGT TATTCGCCGC AGTTCGCTGC TTACTGAAAA GCTGCTGAGA
    CTTCTCTCCC TTATCTCTAT TGCCCTTCCT GATAATAAGG CTTCTGAAAC AGCAGGCAAT
    CAGAGCAATT CTATTACCCC AGCACCTGCA CCTACGCCTG CAACTGCACC AGCCCCTGTG
    CCTGTACCTG CTGTAACAGT TCCAGTTGTT GTGAGCACAA ACATAGTGAC AACTGTTACA
    ACCAAAAGTG GAAAATCTCC TGCAAAATCA AGTGAAGTCA GCAATGCACC AGATAAGATG
    GCAGCTTCTG GCCTTACAGA GAAACAGCTG CAGTTGTCTG TTGAGGTTCT GACATCACAC
    TCGTGTTCAG AAGAAGGACT GGAGGATGCC GCCAATGTTC TACTTCAGCT TTCCAGAGGC
    GACCCCAGTA CACGGGACAC TGTACTGAAA CTTCTGCTTA GCGGAGCACA TCACTTGGGG
    TTTACTCTCT GCAAACAGAT AGGTACACTA CTAGCAGAGC TCCGGGAATA TAACCTAGAG
    CAGCAGCGTC GTGCACAGTG TGAAGTTCTT TCTCCAGATG GTATGCCTGA GGAGCAACCC
    CACAGCACAA AGCCAAAAGG CAAAATGCAG AGCAGTGTTA GGTCTCTCCC CACCCCACCT
    ATGCGATCCA GGTTCGACAT TGCAGAGAAT GTGGTGATTG TGGCTTCCCA AAAGCGTCCA
    CTGGGAGGAA GAGAGCTGCA GCTCCCCTCA ATGTCTATGC TGACGTCAAA GACTTCCACA
    CAAAAATTCT TTCTTCGAGT GCTGCAGGTT ATCATCCAGC TTCGCGATGA CACTCGCAGA
    GCCAACAAGA AGGCCAAGCA GACCAGCAGA TTAGGCACTG CCAGTCTGGC CTCGGCTAGC
    AGCATCCAAG CAGCTGTGCA GCAGCTGGAG GCTGAGGCTG ATGCCATTAT ACAAATGGTA
    CGTGAGGGTC AAAGGGCGCG GAGACAGCTG CAAACTGCAA CGGCGGAGGG TTCTGCTCGC
    AGGGAGGAGC AGCCTATGGA TGTTGACCAG CCTTCGCCTG CTACACAGGA GCCTCAGTCA
    GTTGCTTCTG AAGGTTCTCA GGCTGCTGAA AAAAAAGAAG AGGAGAGGTT ACCAGAGTTA
    CCGCTGTTAA GTGAGCAGCT AAGTTTAGAT GAGCTGTGGG ACATGTTGGG AGAGTGCCTG
    AAGGAACTTG AGGAATCTCA TGACCAGCAT GCCGTATTAG TGTTACAGCC AGCCGTAGAA
    GCCTTTTTCC TTGTTCATGC AACAGAGCGG GAGAGCAAGC CTCCTGTGCG GGACACTCGG
    GAAAGCCAGT TATCTCACAT TAAGGATGAA CCACCTCCCC TGTCTCCTGC ACCCCTCACT
    CCAGCCACAC CTTCTTCCTT GGATCCTTTC TTCTCTCGTG AACCTTCATC TATGCACATT
    TCCTCTAGTT TGCCTCCTGA CACTCAGAAG TTCCTAAGAT TTGCTGAGAC TCATCGGACT
    GTATTAAATC AGATCCTTCG CCAGTCCACC ACACACCTAG CTGATGGGCC CTTTGCTGTG
    CTTGTAGACT ATATCAGAGT CCTTGACTTT GATGTTAAGC GCAAATATTT CCGACAGGAG
    TTGGAGCGAC TGGATGAAGG GCTAAGAAAA GAGGATATGG CAGTGCATGT GCGGCGAGAT
    CATGTGTTTG AGGATTCCTA CCGGGAGCTG CACCGCAAAT CCCCTGAAGA AATGAAGAAT
    AGATTGTACA TAGTATTTGA AGGAGAAGAA GGGCAGGATG CAGGTGGGCT GCTTCGGGAG
    TGGTACATGA TTATCTCCAG GGAGATGTTC AATCCCATGT ATGCTTTGTT CCGCACTTCC
    CCAGGTGACC GCGTCACCTA CACTATTAAT CCTTCCTCCC ACTGTAACCC TAACCACCTC
    AGCTACTTCA AGTTTGTGGG CCGGATAGTT GCCAAAGCGG TTTATGACAA CCGGCTACTG
    GAGTGTTACT TCACTAGGTC CTTCTACAAG CATATATTGG GCAAGTCTGT CAGATACACA
    GACATGGAGA GTGAAGATTA TCACTTCTAT CAGGGTCTGG TGTACTTGCT TGAAAATGAT
    GTCTCCACAC TGGGATATGA CTTGACTTTC AGCACTGAGG TTCAAGAATT TGGTGTCTGT
    GAAGTCCGAG ACCTAAAACC AAACGGTGCA AACATTCTGG TAACAGAAGA AAACAAAAAG
    GAGTATGTTC ATCTTGTGTG CCAGATGAAG ATGACAGGTG CAATACGTAA GCAATTGGCT
    GCCTTCCTTG AGGGATTTTA TGAGATAATC CCCAAACGTC TGATCTCCAT CTTCACTGAG
    CAGGAATTGG AACTGCTTAT CTCTGGCCTT CCCACCATAG ATATTGATGA CCTAAAATCA
    AACACTGAGT ATCACAAATA CCAGTCTAAC TCCATCCAGA TTCAGTGGTT CTGGAGAGCG
    TTGCGCTCAT TCGATCAAGC TGACCGGGCA AAGTTCCTTC AGTTTGTTAC TGGAACTTCA
    AAGGTCCCAT TACAGGGTTT TGCTGCGCTG GAAGGAATGA ACGGCATCCA GAAATTCCAA
    ATCCACAGAG ATGATAGATC TACAGACAGG CTGCCTTCTG CGCACACGTG CTTCAACCAG
    TTGGACCTTC CAGCCTATGA GAGTTACGAG AAGCTACGTC ACATGCTGCT GTTAGCCATC
    CAGGAGTGCT CTGAGGGCTT TGGCCTTGCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65594
    Clone ID Related Accession (Same CDS sequence) XM_018096571.2
    Accession Version XM_018096571.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096571.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    12661
    12721
    12781
    12841
    12901
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    13021
    13081
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGGTGCTG ATGGATATGG GTTTCACAAG GGAGCATGCT
    CTGGAAGCTC TGCTTAACAC GAGTACTATG GAGCAGGCCA CTGAATATCT CCTCACCCAC
    CCTCCACCTC TTATTGGCGG AGTTGTACGG GACTTAAGTA TGTCAGAGGA AGATCAGATG
    CTGAGAGCCA TTGCCATGTC GCTGGGACAA GATATCCCTA TGGAACAGAG GGCAGAACTG
    CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG GAGGAACGCA AGGCACGAGA AAAACAAGAG
    GAAGAGGAAG CTAAATGCTT GGAAAAGTTT CAAGATGCAG AACCCCTGGA ACAGGATGAG
    CTTCAAGGAT TCACAGACAC CATGCTCCCT GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA
    GACACAGTCT ATAGAGTGTG TGATCTTATA ATGACTGCCA TAAAGCGCAA TGGGGCAGAC
    TATCGAGACA TGATTCTAAA ACAAGTGGTT AACCAGGTAT GGGAAGCCGC TGATGTTCTT
    ATTAAAGCTG CACTGCCGCT GACCACAAGT GACACAAAAA CTGTGTCTGA GTGGATCAGC
    CAAATGGCTA CTCTTCCTCA AGCTTCTAAC TTGGCCACTC GCATCCTTTT GCTCACACTG
    CTGTTTGAGG AGCTAAAACT GCCATGTGCT AGAGTTGTTG AAACAAGTGG AATACTTAAC
    GTACTAATTA AGCTGCTTGA AGTGGTTCAG CCATGTTTGC AGGCAGCCAA GGAGCAAAAG
    GAAGTTCAGA CACCAAAGTG GATTACACCA GTCCTCCTCT TAATAGATTT TTATGAGAAG
    ACCTCAATTT CTTCTAAAAG AAGAATGCAA ATGAACAGGT ACCTTCAAGC TAATGGAAAC
    AACTGGCGAT GGTTTGATGA TCGGTCTGGC CGTTGGTGCA GCTACAGTGC TAGTAACAAC
    AGCACTATTG ATGCAGCCTG GAAAGCAGGA GAAACATGTG TGCGATTTAC AGCTGGCCGT
    CGAAGATATA CTGTGCAGTT TACCACAATG GTTCAGGTAA ATGAAGAGAC CGGCAATAGA
    CGACCTGTCA TGCTGACTCT CTTGCGTGTT CCGAGACTTT TAAAGAGTGG AAAAAATGGA
    AATGGACAGG AACTTGAAAA GACACTTGAG GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA
    GACGAAAAAG TGCCTGAGCC CATTCCAGTT CCTGAAACCT CTTCCACTGA GAAAGATGCA
    CCAACTGAAG AGCCCAAAGT TGGAGACATA GTAATTCAGG GTCTGGCTGA AGAAATGGTG
    ACCGTCCTTA TACGTTCCTG CGTCGGCATG CTTGGTGTTC CTGTTGATCC AGATACTCTT
    CATGCAACCT TGCGTCTTTG CCTGCGTCTC ACACGTGACC ACAAATATGC CATGATGTTT
    GCAGAGCTTA AGAGCACTCG AATGATCCTC AGCTTAACTC AAAGCTCTGG ATTCAATGGC
    TTTACTCCCC TTGTGACGCT CCTATTTAGG CACATCATAG AGGATCCTTG CACCCTCCAG
    CACACCATGG AAAAGGTTGT ACGGTCTGCA GCAACTAGTG GGGCAGGGAG TACCACATCT
    GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT GAAATCAACT ATATACTTAG AGTACTGGGT
    CCAGCAGCTT GTCGAAACCC TGACATTTTC ACTGAAGTGG CAAACAGTTG CATTCGCATT
    GCTCTCCCTG CACCTCGTGG ATCTGGAACT GCCTCTGATG ATGAGTTTGA GAACCTTCGG
    ATCAAAGGTC CTAATGCAGT TCAGTTGGTT AAAACCACCC CTATAAAGCC CTCCACCTTG
    CCAGTCATCC CTGATACAAT TAAGGAAGTA ATCTATGACA TGCTGAATGC ACTGGCAGCT
    TACCACGCCC CTGAGGAAGT ATCTGAAAAG TCTGAGCAAA AACCTGTTGG CATGAACCAG
    GAAGTCAGCC AGATCCTACA GGACATGGGT GATGAGGTTT TCCAGCAATA TCGGGACTTA
    ACCAGACAGG GAAGCGACTT TGATGGTCAG ACCAGTTTTG CCATCAACGC GCGAGTCTTT
    ACTGCTGACA GCACAGCTGC CGAAACACCT CAGTCTGGTA CACCTCAAGG TGAAACTGCC
    TTAACACCTG AAGAAAACCG AGAAGGGTTA AAGAAAGATA AGGAAGGAGA CCGTAGTTCA
    GAAGATGGAA AGCAGAAGGC AAAAGGTAGT AAGCCTTTGC TGCCTACCTC TTCCATTCTT
    CGACTACTTG CTGAACTTGT CAGGTCCTAT GTCGGAATAG CCTCGCTGAT CGCCACCTAC
    AATTACACTG CAGGACAATC TGAACTAATT AAAGAGGATT GCAGTGTATT GGCGTTTGTG
    CTGGATCACC TGCTACCCCA CACACATAAT GCAGAGGACA AGGACACACC AGCCCTAGCC
    CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA GGAAGTGGCA CTGATGCCCA GGTTGCACTA
    GTGAATGAAG TAAAGGCAGC TCTTGGCAGA GCCCTAGCAA TGGCTGAGAG CACGGAGAAG
    CATGCTAGGT TGCAGGCTGT GATGTGCATT ATCAGTACCA TTATGGAGTC ATGTCCATCC
    ACCTCCAGCT TCTACAGCAC AGCAACCACC AAAACCCAGC ACAATGGCAT GAACAACATC
    ATCAGACTCT TTTTGAAAAA GGGGCTGGTT AGTGATTTAG CCAGGGTCCC TCACAGCCTT
    GATTTATCCA GTCCAAATAT GGCAAACACC GTGAATGCAG CACTGAAGCC ACTCGAGACA
    CTGTCTCGCA TTGTCAATCA ACCCAGCAGT TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG
    AATAAATCTG AGGCAGATAC ACATGGAACA GCCCAGGATC CCAACAGTAA TCAGCCAGAT
    ACAGGTGAAA CAGGAACAGA AGTGCAGGAA GAGGACCCAG ATGCTTCTCA TGCTGAAGTA
    GCTGATGGAG ACATCATGGA TGGAGAGACA GAGACTGATG CTGTGGTTAT TGCTGGCCAG
    CCTGAAGTCC TTACTACACA AGAAATGCAG GTAGAGAATG AGCTTGAAGA TCTGATAGAT
    GAACTTCTTG AAAGGGATGG TGCTGCAGGT AACCGGGTCG GAGAAGATGA ATCACAGGAA
    GATGTGCTGA TGGATGAGCC TCCTACTAAC CTCAGCCAAG CCTCCACTCT TCAGGCTAAT
    CGAGAAGACT CTATGAACAT CTTAGACCCA GAGGATGAGG AAGAGCACAC CCAAGAAGAG
    GACAGCAGTG GCAGCAATGA AGATGAAGAT GACAGCCAGG AAGAGGAGGA GGAAGAGGAA
    GATGAGGACG ATCAGGATGA TGAGGAGGGT GAGGAAGGGG ATGAGGATGA TGATGATGAC
    TGTTCAGAGA TGGAACTGGA TGAGGATTAT CCAGACATGA ATGCCTCACC TCTTGTACGC
    TTTGAACGTT TTGACAGAGA AGATGATTTA ATCATAGAGT TTGATAACAT GTTCTCCAGT
    GCCACTGAGA TCCCTCCATC CCCTGGTAAC ATCCCTACTA CACACCCCCT AATGGTGCGA
    CACGCTGATC ACGGCTCCTT GACTCTTGGC AGTGGCACTT CCACTACTCG CCTGGCACAG
    GGCATCGGGC GCAGCCAGAG AACACTCCGA CAACTCACAG CCAACACCGG TCACACAATA
    CATGTTCATT ATCCTGGCAA CAGGCAGCCA AATCCACCTT TAATCCTCCA GAGGTTGCTG
    GGGCCCTCAG CCGCTGCTGA CATCTTGCAA CTAAGCAGCA GTCTTCCTCT GCAGAGTCGT
    GGCCGAGCAC GTCTACTTGT TGGCAACGAT GATGTCCACA TCATAGCACG CTCTGATGAT
    GAGATGCTTG ATGATTTTTT CCATGATCAG AGTACAGCCA CAAGTCAGGC TGGCACACTT
    TCCAGCATTC CAACTGCGCT GACCCGCTGG ACAGAAGAGT GTAAGGTCTT GGATGCTGAA
    AGCATGCATG ACTGTGTATC TGTGGTCAAA GTCCCAATCA TCAATCATTT GGAATCTTTG
    CGTGATGAGG AGCTTGAGGA GCGCAGGGAA AAAAGAAGGA AACAACTAGC AGAAGAGGAG
    GCCAAGATGG CAGAGAAATC CAAGGAGGAG AAGGAAGCAA AGGAGCAAAA TTCACAGGGT
    GCCAATTCAA AGCAGAATAC CCCATTTGAA CTGAGCCTTG CAGATCAAGA TGGAAGTGGT
    CGTCCTGAAA ATTTCCCAAC TGCACCATCT TCTGCAGAAG GAACTCCAAC AAGTCCTGAA
    ATTGTTCGTT TAGATCCCAC CCTAAGGGCT ACTGAGGCTA CTCTGGCAGA GCTGACTCAG
    GGTCTGCCTG CTGATACAGG CACGGAGGAT GTTGGGCAAC AACTCATGCC ATCTCACCAA
    GAGGACTCTG GACCAGGACG GCCAAGTGTA GAGGGGGATG CCACACAAAT GGAGCTATCG
    CCAGCACCCA CTATAACCTC CTTATCACCA GAGCGAGCAG AGGATTCAGA TGCTTTGACA
    GCTGTTAGTA GCCAACTAGA GGGCTCGCCT ATGGACACCA GCAGTCTTGC CTCTGGGCCC
    TTAGAGGATG GCGTAGGGGA GGCACCTGGA ATTGTTAATC CAGAGCAGCC TCTTGTGGTT
    GGCAGTAGCA TTGCTGTTGG ACAGCAACCA GTGGAACCAC CGACGACCTG TGAAACCCAA
    CCAGTAGTTT CTCAACACAG TGATAGCTCA CAAATTGCCC CTTCCTCTGA GTTGCCTTCC
    ACAAGAGAAA GTGCTGTGAC CTTGTTGGCT ACAGATTCTA GTGGTATCCT TGAAGAACCT
    TTGCCTTCTA CAAGCAGTGA AGAGGAAGAC CCATTAGCAG GCATAAATCT TCCAGAGGGT
    GTGGATCCTT CTTTCCTAGC GGCTCTTCCT GAAGACATCC GCAGAGAGGT GCTGCAGAAT
    CAGTTGGGCA TACGGCCGCC ATCGCGACCT CCACCCACTG CTGCCCCAGT TATTCCTCCT
    CCTGTGGTTG CTAATGCTGG TGTCACGGAG GTCAGCCCTG AGTTCTTGGC AGCGTTGCCA
    CCAGCCATTC AGGAAGAGGT CCTAGCACAG CAGCGAGCAG AACAGCAGAG GCGGGAACTT
    GCTCAGACCA CTACGTCTGA TACACCCATG GATCCTGTGA CTTTCATTCA GACGTTACCT
    TCTGATCTAC GTCGTAGTGT GTTAGAAGAC ATGGAGGACA GTGTTTTAGC TGTGATGCCA
    CCTGACATTG CTGCAGAGGC ACAGGCTCTA AGGCGAGAGC AAGAAGCTCG GCAGAGGCAA
    CTAATGCATG AACGACTGTT CGGGCACAGT AGCACATCTG CACTCTCTGC CATCCTTAGG
    AGTCCTGCTT TCACGAGTCG CTTGAGTGGA AACCGAGGTG TTCAGTACAC ACGGTTGGCT
    GTACAGAGAG GGAACACTTT CCAAATGGGA AGCAGCAGTA CACATACCAG ACCAACCGGT
    AGTAATGTTG ACAGCCTACT ACGATTACGA GGCCGACTAT TACTTGATCA TGAAGCCCTC
    TCCTGTCTGC TTGTCCTACT GTTTGTTGAT GAACCCAAGC TAAACACCAG TCGCTTGCAC
    CGTGTTCTAC GCAACCTCTG CTACCATGCA CAAACAAGGA ACTGGGTGAT TAGAAGCTTG
    TTGTCTATAC TGCAGCGTAG CAGTGAAAGT GAGCTTTGTA TAGAAAGCCC AAAGACTTCT
    ATTGGGGGCA CTGATAAACG GGGTGCAAAA ACAAGTCATG ACAACCGCCC TTTAGAATTT
    CTGCATAAGA TGGAATCGAA GAATTCCAAC CAGCTCTCCT GGCTTTCTGT ATCCATGGAC
    GCTGCTCTTG GCTGCCGTAC GAACATCTTC CAAATCCAGA GGTCCCTAGG ACGCAAGCAT
    ACAGAAAAGC ATTCAGCCTG TGGTTCTACT GTCCACATCC ATCCCCAAGC AGCCCCTGTA
    GTGTGTAGAC ATGTGCTTGA CACACTAATA CAGCTAGCTA AGGTGTTTCC CAGTCATTTC
    ACACAGCAGC GAACCAAAGA ATGTAGTGTG GAAAACAGTG ACAGCAGGGA GCGGAGTATA
    AAACTTACTT GTAGTCCGTG TCTTGCACCA TCTAGTAGTA TTGGAGCAAG CAACATCTCC
    ACAGATTTCT GGGACTTGCT AGTCAAGCTA GACAACATGA ATGTGAGCCG GAAGGGGAAA
    ACGTCTATAA AATCTGTGCC AGTGAGCTCT GGTTCTGAAG GCGAGAGCAA TCAGTGCAGC
    CTGGAGGCCT CCCCCTTAGG ACAGCTTATG AATATGCTGT CTCATTCTGT TATTCGCCGC
    AGTTCGCTGC TTACTGAAAA GCTGCTGAGA CTTCTCTCCC TTATCTCTAT TGCCCTTCCT
    GATAATAAGG CTTCTGAAAC AGCAGGCAAT CAGAGCAATT CTATTACCCC AGCACCTGCA
    CCTACGCCTG CAACTGCACC AGCCCCTGTG CCTGTACCTG CTGTAACAGT TCCAGTTGTT
    GTGAGCACAA ACATAGTGAC AACTGTTACA ACCAAAAGTG GAAAATCTCC TGCAAAATCA
    AGTGAAGTCA GCAATGCACC AGATAAGATG GCAGCTTCTG GCCTTACAGA GAAACAGCTG
    CAGTTGTCTG TTGAGGTTCT GACATCACAC TCGTGTTCAG AAGAAGGACT GGAGGATGCC
    GCCAATGTTC TACTTCAGCT TTCCAGAGGC GACCCCAGTA CACGGGACAC TGTACTGAAA
    CTTCTGCTTA GCGGAGCACA TCACTTGGGG TTTACTCTCT GCAAACAGAT AGGTACACTA
    CTAGCAGAGC TCCGGGAATA TAACCTAGAG CAGCAGCGTC GTGCACAGTG TGAAGTTCTT
    TCTCCAGATG GTATGCCTGA GGAGCAACCC CACAGCACAA AGCCAAAAGG CAAAATGCAG
    AGCAGGTTCG ACATTGCAGA GAATGTGGTG ATTGTGGCTT CCCAAAAGCG TCCACTGGGA
    GGAAGAGAGC TGCAGCTCCC CTCAATGTCT ATGCTGACGT CAAAGACTTC CACACAAAAA
    TTCTTTCTTC GAGTGCTGCA GGTTATCATC CAGCTTCGCG ATGACACTCG CAGAGCCAAC
    AAGAAGGCCA AGCAGACCAG CAGATTAGGC ACTGCCAGTC TGGCCTCGGC TAGCAGCATC
    CAAGCAGCTG TGCAGCAGCT GGAGGCTGAG GCTGATGCCA TTATACAAAT GGTACGTGAG
    GGTCAAAGGG CGCGGAGACA GCTGCAAACT GCAACGGCGG AGGGTTCTGC TCGCAGGGAG
    GAGCAGCCTA TGGATGTTGA CCAGCCTTCG CCTGCTACAC AGGAGCCTCA GTCAGTTGCT
    TCTGAAGGTT CTCAGGCTGC TGAAAAAAAA GAAGAGGAGA GGTTACCAGA GTTACCGCTG
    TTAAGTGAGC AGCTAAGTTT AGATGAGCTG TGGGACATGT TGGGAGAGTG CCTGAAGGAA
    CTTGAGGAAT CTCATGACCA GCATGCCGTA TTAGTGTTAC AGCCAGCCGT AGAAGCCTTT
    TTCCTTGTTC ATGCAACAGA GCGGGAGAGC AAGCCTCCTG TGCGGGACAC TCGGGAAAGC
    CAGTTATCTC ACATTAAGGA TGAACCACCT CCCCTGTCTC CTGCACCCCT CACTCCAGCC
    ACACCTTCTT CCTTGGATCC TTTCTTCTCT CGTGAACCTT CATCTATGCA CATTTCCTCT
    AGTTTGCCTC CTGACACTCA GAAGTTCCTA AGATTTGCTG AGACTCATCG GACTGTATTA
    AATCAGATCC TTCGCCAGTC CACCACACAC CTAGCTGATG GGCCCTTTGC TGTGCTTGTA
    GACTATATCA GAGTCCTTGA CTTTGATGTT AAGCGCAAAT ATTTCCGACA GGAGTTGGAG
    CGACTGGATG AAGGGCTAAG AAAAGAGGAT ATGGCAGTGC ATGTGCGGCG AGATCATGTG
    TTTGAGGATT CCTACCGGGA GCTGCACCGC AAATCCCCTG AAGAAATGAA GAATAGATTG
    TACATAGTAT TTGAAGGAGA AGAAGGGCAG GATGCAGGTG GGCTGCTTCG GGAGTGGTAC
    ATGATTATCT CCAGGGAGAT GTTCAATCCC ATGTATGCTT TGTTCCGCAC TTCCCCAGGT
    GACCGCGTCA CCTACACTAT TAATCCTTCC TCCCACTGTA ACCCTAACCA CCTCAGCTAC
    TTCAAGTTTG TGGGCCGGAT AGTTGCCAAA GCGGTTTATG ACAACCGGCT ACTGGAGTGT
    TACTTCACTA GGTCCTTCTA CAAGCATATA TTGGGCAAGT CTGTCAGATA CACAGACATG
    GAGAGTGAAG ATTATCACTT CTATCAGGGT CTGGTGTACT TGCTTGAAAA TGATGTCTCC
    ACACTGGGAT ATGACTTGAC TTTCAGCACT GAGGTTCAAG AATTTGGTGT CTGTGAAGTC
    CGAGACCTAA AACCAAACGG TGCAAACATT CTGGTAACAG AAGAAAACAA AAAGGAGTAT
    GTTCATCTTG TGTGCCAGAT GAAGATGACA GGTGCAATAC GTAAGCAATT GGCTGCCTTC
    CTTGAGGGAT TTTATGAGAT AATCCCCAAA CGTCTGATCT CCATCTTCAC TGAGCAGGAA
    TTGGAACTGC TTATCTCTGG CCTTCCCACC ATAGATATTG ATGACCTAAA ATCAAACACT
    GAGTATCACA AATACCAGTC TAACTCCATC CAGATTCAGT GGTTCTGGAG AGCGTTGCGC
    TCATTCGATC AAGCTGACCG GGCAAAGTTC CTTCAGTTTG TTACTGGAAC TTCAAAGGTC
    CCATTACAGG GTTTTGCTGC GCTGGAAGGA ATGAACGGCA TCCAGAAATT CCAAATCCAC
    AGAGATGATA GATCTACAGA CAGGCTGCCT TCTGCGCACA CGTGCTTCAA CCAGTTGGAC
    CTTCCAGCCT ATGAGAGTTA CGAGAAGCTA CGTCACATGC TGCTGTTAGC CATCCAGGAG
    TGCTCTGAGG GCTTTGGCCT TGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952060.2
    CDS671..13777
    Translation

    Target ORF information:

    RefSeq Version XM_018096571.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096571.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGGTGCTG ATGGATATGG GTTTCACAAG GGAGCATGCT
    CTGGAAGCTC TGCTTAACAC GAGTACTATG GAGCAGGCCA CTGAATATCT CCTCACCCAC
    CCTCCACCTC TTATTGGCGG AGTTGTACGG GACTTAAGTA TGTCAGAGGA AGATCAGATG
    CTGAGAGCCA TTGCCATGTC GCTGGGACAA GATATCCCTA TGGAACAGAG GGCAGAACTG
    CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG GAGGAACGCA AGGCACGAGA AAAACAAGAG
    GAAGAGGAAG CTAAATGCTT GGAAAAGTTT CAAGATGCAG AACCCCTGGA ACAGGATGAG
    CTTCAAGGAT TCACAGACAC CATGCTCCCT GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA
    GACACAGTCT ATAGAGTGTG TGATCTTATA ATGACTGCCA TAAAGCGCAA TGGGGCAGAC
    TATCGAGACA TGATTCTAAA ACAAGTGGTT AACCAGGTAT GGGAAGCCGC TGATGTTCTT
    ATTAAAGCTG CACTGCCGCT GACCACAAGT GACACAAAAA CTGTGTCTGA GTGGATCAGC
    CAAATGGCTA CTCTTCCTCA AGCTTCTAAC TTGGCCACTC GCATCCTTTT GCTCACACTG
    CTGTTTGAGG AGCTAAAACT GCCATGTGCT AGAGTTGTTG AAACAAGTGG AATACTTAAC
    GTACTAATTA AGCTGCTTGA AGTGGTTCAG CCATGTTTGC AGGCAGCCAA GGAGCAAAAG
    GAAGTTCAGA CACCAAAGTG GATTACACCA GTCCTCCTCT TAATAGATTT TTATGAGAAG
    ACCTCAATTT CTTCTAAAAG AAGAATGCAA ATGAACAGGT ACCTTCAAGC TAATGGAAAC
    AACTGGCGAT GGTTTGATGA TCGGTCTGGC CGTTGGTGCA GCTACAGTGC TAGTAACAAC
    AGCACTATTG ATGCAGCCTG GAAAGCAGGA GAAACATGTG TGCGATTTAC AGCTGGCCGT
    CGAAGATATA CTGTGCAGTT TACCACAATG GTTCAGGTAA ATGAAGAGAC CGGCAATAGA
    CGACCTGTCA TGCTGACTCT CTTGCGTGTT CCGAGACTTT TAAAGAGTGG AAAAAATGGA
    AATGGACAGG AACTTGAAAA GACACTTGAG GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA
    GACGAAAAAG TGCCTGAGCC CATTCCAGTT CCTGAAACCT CTTCCACTGA GAAAGATGCA
    CCAACTGAAG AGCCCAAAGT TGGAGACATA GTAATTCAGG GTCTGGCTGA AGAAATGGTG
    ACCGTCCTTA TACGTTCCTG CGTCGGCATG CTTGGTGTTC CTGTTGATCC AGATACTCTT
    CATGCAACCT TGCGTCTTTG CCTGCGTCTC ACACGTGACC ACAAATATGC CATGATGTTT
    GCAGAGCTTA AGAGCACTCG AATGATCCTC AGCTTAACTC AAAGCTCTGG ATTCAATGGC
    TTTACTCCCC TTGTGACGCT CCTATTTAGG CACATCATAG AGGATCCTTG CACCCTCCAG
    CACACCATGG AAAAGGTTGT ACGGTCTGCA GCAACTAGTG GGGCAGGGAG TACCACATCT
    GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT GAAATCAACT ATATACTTAG AGTACTGGGT
    CCAGCAGCTT GTCGAAACCC TGACATTTTC ACTGAAGTGG CAAACAGTTG CATTCGCATT
    GCTCTCCCTG CACCTCGTGG ATCTGGAACT GCCTCTGATG ATGAGTTTGA GAACCTTCGG
    ATCAAAGGTC CTAATGCAGT TCAGTTGGTT AAAACCACCC CTATAAAGCC CTCCACCTTG
    CCAGTCATCC CTGATACAAT TAAGGAAGTA ATCTATGACA TGCTGAATGC ACTGGCAGCT
    TACCACGCCC CTGAGGAAGT ATCTGAAAAG TCTGAGCAAA AACCTGTTGG CATGAACCAG
    GAAGTCAGCC AGATCCTACA GGACATGGGT GATGAGGTTT TCCAGCAATA TCGGGACTTA
    ACCAGACAGG GAAGCGACTT TGATGGTCAG ACCAGTTTTG CCATCAACGC GCGAGTCTTT
    ACTGCTGACA GCACAGCTGC CGAAACACCT CAGTCTGGTA CACCTCAAGG TGAAACTGCC
    TTAACACCTG AAGAAAACCG AGAAGGGTTA AAGAAAGATA AGGAAGGAGA CCGTAGTTCA
    GAAGATGGAA AGCAGAAGGC AAAAGGTAGT AAGCCTTTGC TGCCTACCTC TTCCATTCTT
    CGACTACTTG CTGAACTTGT CAGGTCCTAT GTCGGAATAG CCTCGCTGAT CGCCACCTAC
    AATTACACTG CAGGACAATC TGAACTAATT AAAGAGGATT GCAGTGTATT GGCGTTTGTG
    CTGGATCACC TGCTACCCCA CACACATAAT GCAGAGGACA AGGACACACC AGCCCTAGCC
    CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA GGAAGTGGCA CTGATGCCCA GGTTGCACTA
    GTGAATGAAG TAAAGGCAGC TCTTGGCAGA GCCCTAGCAA TGGCTGAGAG CACGGAGAAG
    CATGCTAGGT TGCAGGCTGT GATGTGCATT ATCAGTACCA TTATGGAGTC ATGTCCATCC
    ACCTCCAGCT TCTACAGCAC AGCAACCACC AAAACCCAGC ACAATGGCAT GAACAACATC
    ATCAGACTCT TTTTGAAAAA GGGGCTGGTT AGTGATTTAG CCAGGGTCCC TCACAGCCTT
    GATTTATCCA GTCCAAATAT GGCAAACACC GTGAATGCAG CACTGAAGCC ACTCGAGACA
    CTGTCTCGCA TTGTCAATCA ACCCAGCAGT TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG
    AATAAATCTG AGGCAGATAC ACATGGAACA GCCCAGGATC CCAACAGTAA TCAGCCAGAT
    ACAGGTGAAA CAGGAACAGA AGTGCAGGAA GAGGACCCAG ATGCTTCTCA TGCTGAAGTA
    GCTGATGGAG ACATCATGGA TGGAGAGACA GAGACTGATG CTGTGGTTAT TGCTGGCCAG
    CCTGAAGTCC TTACTACACA AGAAATGCAG GTAGAGAATG AGCTTGAAGA TCTGATAGAT
    GAACTTCTTG AAAGGGATGG TGCTGCAGGT AACCGGGTCG GAGAAGATGA ATCACAGGAA
    GATGTGCTGA TGGATGAGCC TCCTACTAAC CTCAGCCAAG CCTCCACTCT TCAGGCTAAT
    CGAGAAGACT CTATGAACAT CTTAGACCCA GAGGATGAGG AAGAGCACAC CCAAGAAGAG
    GACAGCAGTG GCAGCAATGA AGATGAAGAT GACAGCCAGG AAGAGGAGGA GGAAGAGGAA
    GATGAGGACG ATCAGGATGA TGAGGAGGGT GAGGAAGGGG ATGAGGATGA TGATGATGAC
    TGTTCAGAGA TGGAACTGGA TGAGGATTAT CCAGACATGA ATGCCTCACC TCTTGTACGC
    TTTGAACGTT TTGACAGAGA AGATGATTTA ATCATAGAGT TTGATAACAT GTTCTCCAGT
    GCCACTGAGA TCCCTCCATC CCCTGGTAAC ATCCCTACTA CACACCCCCT AATGGTGCGA
    CACGCTGATC ACGGCTCCTT GACTCTTGGC AGTGGCACTT CCACTACTCG CCTGGCACAG
    GGCATCGGGC GCAGCCAGAG AACACTCCGA CAACTCACAG CCAACACCGG TCACACAATA
    CATGTTCATT ATCCTGGCAA CAGGCAGCCA AATCCACCTT TAATCCTCCA GAGGTTGCTG
    GGGCCCTCAG CCGCTGCTGA CATCTTGCAA CTAAGCAGCA GTCTTCCTCT GCAGAGTCGT
    GGCCGAGCAC GTCTACTTGT TGGCAACGAT GATGTCCACA TCATAGCACG CTCTGATGAT
    GAGATGCTTG ATGATTTTTT CCATGATCAG AGTACAGCCA CAAGTCAGGC TGGCACACTT
    TCCAGCATTC CAACTGCGCT GACCCGCTGG ACAGAAGAGT GTAAGGTCTT GGATGCTGAA
    AGCATGCATG ACTGTGTATC TGTGGTCAAA GTCCCAATCA TCAATCATTT GGAATCTTTG
    CGTGATGAGG AGCTTGAGGA GCGCAGGGAA AAAAGAAGGA AACAACTAGC AGAAGAGGAG
    GCCAAGATGG CAGAGAAATC CAAGGAGGAG AAGGAAGCAA AGGAGCAAAA TTCACAGGGT
    GCCAATTCAA AGCAGAATAC CCCATTTGAA CTGAGCCTTG CAGATCAAGA TGGAAGTGGT
    CGTCCTGAAA ATTTCCCAAC TGCACCATCT TCTGCAGAAG GAACTCCAAC AAGTCCTGAA
    ATTGTTCGTT TAGATCCCAC CCTAAGGGCT ACTGAGGCTA CTCTGGCAGA GCTGACTCAG
    GGTCTGCCTG CTGATACAGG CACGGAGGAT GTTGGGCAAC AACTCATGCC ATCTCACCAA
    GAGGACTCTG GACCAGGACG GCCAAGTGTA GAGGGGGATG CCACACAAAT GGAGCTATCG
    CCAGCACCCA CTATAACCTC CTTATCACCA GAGCGAGCAG AGGATTCAGA TGCTTTGACA
    GCTGTTAGTA GCCAACTAGA GGGCTCGCCT ATGGACACCA GCAGTCTTGC CTCTGGGCCC
    TTAGAGGATG GCGTAGGGGA GGCACCTGGA ATTGTTAATC CAGAGCAGCC TCTTGTGGTT
    GGCAGTAGCA TTGCTGTTGG ACAGCAACCA GTGGAACCAC CGACGACCTG TGAAACCCAA
    CCAGTAGTTT CTCAACACAG TGATAGCTCA CAAATTGCCC CTTCCTCTGA GTTGCCTTCC
    ACAAGAGAAA GTGCTGTGAC CTTGTTGGCT ACAGATTCTA GTGGTATCCT TGAAGAACCT
    TTGCCTTCTA CAAGCAGTGA AGAGGAAGAC CCATTAGCAG GCATAAATCT TCCAGAGGGT
    GTGGATCCTT CTTTCCTAGC GGCTCTTCCT GAAGACATCC GCAGAGAGGT GCTGCAGAAT
    CAGTTGGGCA TACGGCCGCC ATCGCGACCT CCACCCACTG CTGCCCCAGT TATTCCTCCT
    CCTGTGGTTG CTAATGCTGG TGTCACGGAG GTCAGCCCTG AGTTCTTGGC AGCGTTGCCA
    CCAGCCATTC AGGAAGAGGT CCTAGCACAG CAGCGAGCAG AACAGCAGAG GCGGGAACTT
    GCTCAGACCA CTACGTCTGA TACACCCATG GATCCTGTGA CTTTCATTCA GACGTTACCT
    TCTGATCTAC GTCGTAGTGT GTTAGAAGAC ATGGAGGACA GTGTTTTAGC TGTGATGCCA
    CCTGACATTG CTGCAGAGGC ACAGGCTCTA AGGCGAGAGC AAGAAGCTCG GCAGAGGCAA
    CTAATGCATG AACGACTGTT CGGGCACAGT AGCACATCTG CACTCTCTGC CATCCTTAGG
    AGTCCTGCTT TCACGAGTCG CTTGAGTGGA AACCGAGGTG TTCAGTACAC ACGGTTGGCT
    GTACAGAGAG GGAACACTTT CCAAATGGGA AGCAGCAGTA CACATACCAG ACCAACCGGT
    AGTAATGTTG ACAGCCTACT ACGATTACGA GGCCGACTAT TACTTGATCA TGAAGCCCTC
    TCCTGTCTGC TTGTCCTACT GTTTGTTGAT GAACCCAAGC TAAACACCAG TCGCTTGCAC
    CGTGTTCTAC GCAACCTCTG CTACCATGCA CAAACAAGGA ACTGGGTGAT TAGAAGCTTG
    TTGTCTATAC TGCAGCGTAG CAGTGAAAGT GAGCTTTGTA TAGAAAGCCC AAAGACTTCT
    ATTGGGGGCA CTGATAAACG GGGTGCAAAA ACAAGTCATG ACAACCGCCC TTTAGAATTT
    CTGCATAAGA TGGAATCGAA GAATTCCAAC CAGCTCTCCT GGCTTTCTGT ATCCATGGAC
    GCTGCTCTTG GCTGCCGTAC GAACATCTTC CAAATCCAGA GGTCCCTAGG ACGCAAGCAT
    ACAGAAAAGC ATTCAGCCTG TGGTTCTACT GTCCACATCC ATCCCCAAGC AGCCCCTGTA
    GTGTGTAGAC ATGTGCTTGA CACACTAATA CAGCTAGCTA AGGTGTTTCC CAGTCATTTC
    ACACAGCAGC GAACCAAAGA ATGTAGTGTG GAAAACAGTG ACAGCAGGGA GCGGAGTATA
    AAACTTACTT GTAGTCCGTG TCTTGCACCA TCTAGTAGTA TTGGAGCAAG CAACATCTCC
    ACAGATTTCT GGGACTTGCT AGTCAAGCTA GACAACATGA ATGTGAGCCG GAAGGGGAAA
    ACGTCTATAA AATCTGTGCC AGTGAGCTCT GGTTCTGAAG GCGAGAGCAA TCAGTGCAGC
    CTGGAGGCCT CCCCCTTAGG ACAGCTTATG AATATGCTGT CTCATTCTGT TATTCGCCGC
    AGTTCGCTGC TTACTGAAAA GCTGCTGAGA CTTCTCTCCC TTATCTCTAT TGCCCTTCCT
    GATAATAAGG CTTCTGAAAC AGCAGGCAAT CAGAGCAATT CTATTACCCC AGCACCTGCA
    CCTACGCCTG CAACTGCACC AGCCCCTGTG CCTGTACCTG CTGTAACAGT TCCAGTTGTT
    GTGAGCACAA ACATAGTGAC AACTGTTACA ACCAAAAGTG GAAAATCTCC TGCAAAATCA
    AGTGAAGTCA GCAATGCACC AGATAAGATG GCAGCTTCTG GCCTTACAGA GAAACAGCTG
    CAGTTGTCTG TTGAGGTTCT GACATCACAC TCGTGTTCAG AAGAAGGACT GGAGGATGCC
    GCCAATGTTC TACTTCAGCT TTCCAGAGGC GACCCCAGTA CACGGGACAC TGTACTGAAA
    CTTCTGCTTA GCGGAGCACA TCACTTGGGG TTTACTCTCT GCAAACAGAT AGGTACACTA
    CTAGCAGAGC TCCGGGAATA TAACCTAGAG CAGCAGCGTC GTGCACAGTG TGAAGTTCTT
    TCTCCAGATG GTATGCCTGA GGAGCAACCC CACAGCACAA AGCCAAAAGG CAAAATGCAG
    AGCAGGTTCG ACATTGCAGA GAATGTGGTG ATTGTGGCTT CCCAAAAGCG TCCACTGGGA
    GGAAGAGAGC TGCAGCTCCC CTCAATGTCT ATGCTGACGT CAAAGACTTC CACACAAAAA
    TTCTTTCTTC GAGTGCTGCA GGTTATCATC CAGCTTCGCG ATGACACTCG CAGAGCCAAC
    AAGAAGGCCA AGCAGACCAG CAGATTAGGC ACTGCCAGTC TGGCCTCGGC TAGCAGCATC
    CAAGCAGCTG TGCAGCAGCT GGAGGCTGAG GCTGATGCCA TTATACAAAT GGTACGTGAG
    GGTCAAAGGG CGCGGAGACA GCTGCAAACT GCAACGGCGG AGGGTTCTGC TCGCAGGGAG
    GAGCAGCCTA TGGATGTTGA CCAGCCTTCG CCTGCTACAC AGGAGCCTCA GTCAGTTGCT
    TCTGAAGGTT CTCAGGCTGC TGAAAAAAAA GAAGAGGAGA GGTTACCAGA GTTACCGCTG
    TTAAGTGAGC AGCTAAGTTT AGATGAGCTG TGGGACATGT TGGGAGAGTG CCTGAAGGAA
    CTTGAGGAAT CTCATGACCA GCATGCCGTA TTAGTGTTAC AGCCAGCCGT AGAAGCCTTT
    TTCCTTGTTC ATGCAACAGA GCGGGAGAGC AAGCCTCCTG TGCGGGACAC TCGGGAAAGC
    CAGTTATCTC ACATTAAGGA TGAACCACCT CCCCTGTCTC CTGCACCCCT CACTCCAGCC
    ACACCTTCTT CCTTGGATCC TTTCTTCTCT CGTGAACCTT CATCTATGCA CATTTCCTCT
    AGTTTGCCTC CTGACACTCA GAAGTTCCTA AGATTTGCTG AGACTCATCG GACTGTATTA
    AATCAGATCC TTCGCCAGTC CACCACACAC CTAGCTGATG GGCCCTTTGC TGTGCTTGTA
    GACTATATCA GAGTCCTTGA CTTTGATGTT AAGCGCAAAT ATTTCCGACA GGAGTTGGAG
    CGACTGGATG AAGGGCTAAG AAAAGAGGAT ATGGCAGTGC ATGTGCGGCG AGATCATGTG
    TTTGAGGATT CCTACCGGGA GCTGCACCGC AAATCCCCTG AAGAAATGAA GAATAGATTG
    TACATAGTAT TTGAAGGAGA AGAAGGGCAG GATGCAGGTG GGCTGCTTCG GGAGTGGTAC
    ATGATTATCT CCAGGGAGAT GTTCAATCCC ATGTATGCTT TGTTCCGCAC TTCCCCAGGT
    GACCGCGTCA CCTACACTAT TAATCCTTCC TCCCACTGTA ACCCTAACCA CCTCAGCTAC
    TTCAAGTTTG TGGGCCGGAT AGTTGCCAAA GCGGTTTATG ACAACCGGCT ACTGGAGTGT
    TACTTCACTA GGTCCTTCTA CAAGCATATA TTGGGCAAGT CTGTCAGATA CACAGACATG
    GAGAGTGAAG ATTATCACTT CTATCAGGGT CTGGTGTACT TGCTTGAAAA TGATGTCTCC
    ACACTGGGAT ATGACTTGAC TTTCAGCACT GAGGTTCAAG AATTTGGTGT CTGTGAAGTC
    CGAGACCTAA AACCAAACGG TGCAAACATT CTGGTAACAG AAGAAAACAA AAAGGAGTAT
    GTTCATCTTG TGTGCCAGAT GAAGATGACA GGTGCAATAC GTAAGCAATT GGCTGCCTTC
    CTTGAGGGAT TTTATGAGAT AATCCCCAAA CGTCTGATCT CCATCTTCAC TGAGCAGGAA
    TTGGAACTGC TTATCTCTGG CCTTCCCACC ATAGATATTG ATGACCTAAA ATCAAACACT
    GAGTATCACA AATACCAGTC TAACTCCATC CAGATTCAGT GGTTCTGGAG AGCGTTGCGC
    TCATTCGATC AAGCTGACCG GGCAAAGTTC CTTCAGTTTG TTACTGGAAC TTCAAAGGTC
    CCATTACAGG GTTTTGCTGC GCTGGAAGGA ATGAACGGCA TCCAGAAATT CCAAATCCAC
    AGAGATGATA GATCTACAGA CAGGCTGCCT TCTGCGCACA CGTGCTTCAA CCAGTTGGAC
    CTTCCAGCCT ATGAGAGTTA CGAGAAGCTA CGTCACATGC TGCTGTTAGC CATCCAGGAG
    TGCTCTGAGG GCTTTGGCCT TGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65595
    Clone ID Related Accession (Same CDS sequence) XM_031892175.1
    Accession Version XM_031892175.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    12481
    12541
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    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGGTGCTG ATGGATATGG GTTTCACAAG GGAGCATGCT
    CTGGAAGCTC TGCTTAACAC GAGTACTATG GAGCAGGCCA CTGAATATCT CCTCACCCAC
    CCTCCACCTC TTATTGGCGG AGTTGTACGG GACTTAAGTA TGTCAGAGGA AGATCAGATG
    CTGAGAGCCA TTGCCATGTC GCTGGGACAA GATATCCCTA TGGAACAGAG GGCAGAACTG
    CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG GAGGAACGCA AGGCACGAGA AAAACAAGAG
    GAAGAGGAAG CTAAATGCTT GGAAAAGTTT CAAGATGCAG AACCCCTGGA ACAGGATGAG
    CTTCAAGGAT TCACAGACAC CATGCTCCCT GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA
    GACACAGTCT ATAGAGTGTG TGATCTTATA ATGACTGCCA TAAAGCGCAA TGGGGCAGAC
    TATCGAGACA TGATTCTAAA ACAAGTGGTT AACCAGGTAT GGGAAGCCGC TGATGTTCTT
    ATTAAAGCTG CACTGCCGCT GACCACAAGT GACACAAAAA CTGTGTCTGA GTGGATCAGC
    CAAATGGCTA CTCTTCCTCA AGCTTCTAAC TTGGCCACTC GCATCCTTTT GCTCACACTG
    CTGTTTGAGG AGCTAAAACT GCCATGTGCT AGAGTTGTTG AAACAAGTGG AATACTTAAC
    GTACTAATTA AGCTGCTTGA AGTGGTTCAG CCATGTTTGC AGGCAGCCAA GGAGCAAAAG
    GAAGTTCAGA CACCAAAGTG GATTACACCA GTCCTCCTCT TAATAGATTT TTATGAGAAG
    ACCTCAATTT CTTCTAAAAG AAGAATGCAA ATGAACAGGT ACCTTCAAGC TAATGGAAAC
    AACTGGCGAT GGTTTGATGA TCGGTCTGGC CGTTGGTGCA GCTACAGTGC TAGTAACAAC
    AGCACTATTG ATGCAGCCTG GAAAGCAGGA GAAACATGTG TGCGATTTAC AGCTGGCCGT
    CGAAGATATA CTGTGCAGTT TACCACAATG GTTCAGGTAA ATGAAGAGAC CGGCAATAGA
    CGACCTGTCA TGCTGACTCT CTTGCGTGTT CCGAGACTTT TAAAGAGTGG AAAAAATGGA
    AATGGACAGG AACTTGAAAA GACACTTGAG GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA
    GACGAAAAAG TGCCTGAGCC CATTCCAGTT CCTGAAACCT CTTCCACTGA GAAAGATGCA
    CCAACTGAAG AGCCCAAAGT TGGAGACATA GTAATTCAGG GTCTGGCTGA AGAAATGGTG
    ACCGTCCTTA TACGTTCCTG CGTCGGCATG CTTGGTGTTC CTGTTGATCC AGATACTCTT
    CATGCAACCT TGCGTCTTTG CCTGCGTCTC ACACGTGACC ACAAATATGC CATGATGTTT
    GCAGAGCTTA AGAGCACTCG AATGATCCTC AGCTTAACTC AAAGCTCTGG ATTCAATGGC
    TTTACTCCCC TTGTGACGCT CCTATTTAGG CACATCATAG AGGATCCTTG CACCCTCCAG
    CACACCATGG AAAAGGTTGT ACGGTCTGCA GCAACTAGTG GGGCAGGGAG TACCACATCT
    GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT GAAATCAACT ATATACTTAG AGTACTGGGT
    CCAGCAGCTT GTCGAAACCC TGACATTTTC ACTGAAGTGG CAAACAGTTG CATTCGCATT
    GCTCTCCCTG CACCTCGTGG ATCTGGAACT GCCTCTGATG ATGAGTTTGA GAACCTTCGG
    ATCAAAGGTC CTAATGCAGT TCAGTTGGTT AAAACCACCC CTATAAAGCC CTCCACCTTG
    CCAGTCATCC CTGATACAAT TAAGGAAGTA ATCTATGACA TGCTGAATGC ACTGGCAGCT
    TACCACGCCC CTGAGGAAGT ATCTGAAAAG TCTGAGCAAA AACCTGTTGG CATGAACCAG
    GAAGTCAGCC AGATCCTACA GGACATGGGT GATGAGGTTT TCCAGCAATA TCGGGACTTA
    ACCAGACAGG GAAGCGACTT TGATGGTCAG ACCAGTTTTG CCATCAACGC GCGAGTCTTT
    ACTGCTGACA GCACAGCTGC CGAAACACCT CAGTCTGGTA CACCTCAAGG TGAAACTGCC
    TTAACACCTG AAGAAAACCG AGAAGGGTTA AAGAAAGATA AGGAAGGAGA CCGTAGTTCA
    GAAGATGGAA AGCAGAAGGC AAAAGGTAGT AAGCCTTTGC TGCCTACCTC TTCCATTCTT
    CGACTACTTG CTGAACTTGT CAGGTCCTAT GTCGGAATAG CCTCGCTGAT CGCCACCTAC
    AATTACACTG CAGGACAATC TGAACTAATT AAAGAGGATT GCAGTGTATT GGCGTTTGTG
    CTGGATCACC TGCTACCCCA CACACATAAT GCAGAGGACA AGGACACACC AGCCCTAGCC
    CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA GGAAGTGGCA CTGATGCCCA GGTTGCACTA
    GTGAATGAAG TAAAGGCAGC TCTTGGCAGA GCCCTAGCAA TGGCTGAGAG CACGGAGAAG
    CATGCTAGGT TGCAGGCTGT GATGTGCATT ATCAGTACCA TTATGGAGTC ATGTCCATCC
    ACCTCCAGCT TCTACAGCAC AGCAACCACC AAAACCCAGC ACAATGGCAT GAACAACATC
    ATCAGACTCT TTTTGAAAAA GGGGCTGGTT AGTGATTTAG CCAGGGTCCC TCACAGCCTT
    GATTTATCCA GTCCAAATAT GGCAAACACC GTGAATGCAG CACTGAAGCC ACTCGAGACA
    CTGTCTCGCA TTGTCAATCA ACCCAGCAGT TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG
    AATAAATCTG AGGCAGATAC ACATGGAACA GCCCAGGATC CCAACAGTAA TCAGCCAGAT
    ACAGGTGAAA CAGGAACAGA AGTGCAGGAA GAGGACCCAG ATGCTTCTCA TGCTGAAGTA
    GCTGATGGAG ACATCATGGA TGGAGAGACA GAGACTGATG CTGTGGTTAT TGCTGGCCAG
    CCTGAAGTCC TTACTACACA AGAAATGCAG GTAGAGAATG AGCTTGAAGA TCTGATAGAT
    GAACTTCTTG AAAGGGATGG TGCTGCAGGT AACCGGGTCG GAGAAGATGA ATCACAGGAA
    GATGTGCTGA TGGATGAGCC TCCTACTAAC CTCAGCCAAG CCTCCACTCT TCAGGCTAAT
    CGAGAAGACT CTATGAACAT CTTAGACCCA GAGGATGAGG AAGAGCACAC CCAAGAAGAG
    GACAGCAGTG GCAGCAATGA AGATGAAGAT GACAGCCAGG AAGAGGAGGA GGAAGAGGAA
    GATGAGGACG ATCAGGATGA TGAGGAGGGT GAGGAAGGGG ATGAGGATGA TGATGATGAC
    TGTTCAGAGA TGGAACTGGA TGAGGATTAT CCAGACATGA ATGCCTCACC TCTTGTACGC
    TTTGAACGTT TTGACAGAGA AGATGATTTA ATCATAGAGT TTGATAACAT GTTCTCCAGT
    GCCACTGAGA TCCCTCCATC CCCTGGTAAC ATCCCTACTA CACACCCCCT AATGGTGCGA
    CACGCTGATC ACGGCTCCTT GACTCTTGGC AGTGGCACTT CCACTACTCG CCTGGCACAG
    GGCATCGGGC GCAGCCAGAG AACACTCCGA CAACTCACAG CCAACACCGG TCACACAATA
    CATGTTCATT ATCCTGGCAA CAGGCAGCCA AATCCACCTT TAATCCTCCA GAGGTTGCTG
    GGGCCCTCAG CCGCTGCTGA CATCTTGCAA CTAAGCAGCA GTCTTCCTCT GCAGAGTCGT
    GGCCGAGCAC GTCTACTTGT TGGCAACGAT GATGTCCACA TCATAGCACG CTCTGATGAT
    GAGATGCTTG ATGATTTTTT CCATGATCAG AGTACAGCCA CAAGTCAGGC TGGCACACTT
    TCCAGCATTC CAACTGCGCT GACCCGCTGG ACAGAAGAGT GTAAGGTCTT GGATGCTGAA
    AGCATGCATG ACTGTGTATC TGTGGTCAAA GTCCCAATCA TCAATCATTT GGAATCTTTG
    CGTGATGAGG AGCTTGAGGA GCGCAGGGAA AAAAGAAGGA AACAACTAGC AGAAGAGGAG
    GCCAAGATGG CAGAGAAATC CAAGGAGGAG AAGGAAGCAA AGGAGCAAAA TTCACAGGGT
    GCCAATTCAA AGCAGAATAC CCCATTTGAA CTGAGCCTTG CAGATCAAGA TGGAAGTGGT
    CGTCCTGAAA ATTTCCCAAC TGCACCATCT TCTGCAGAAG GAACTCCAAC AAGTCCTGAA
    ATTGTTCGTT TAGATCCCAC CCTAAGGGCT ACTGAGGCTA CTCTGGCAGA GCTGACTCAG
    GGTCTGCCTG CTGATACAGG CACGGAGGAT GTTGGGCAAC AACTCATGCC ATCTCACCAA
    GAGGACTCTG GACCAGGACG GCCAAGTGTA GAGGGGGATG CCACACAAAT GGAGCTATCG
    CCAGCACCCA CTATAACCTC CTTATCACCA GAGCGAGCAG AGGATTCAGA TGCTTTGACA
    GCTGTTAGTA GCCAACTAGA GGGCTCGCCT ATGGACACCA GCAGTCTTGC CTCTGGGCCC
    TTAGAGGATG GCGTAGGGGA GGCACCTGGA ATTGTTAATC CAGAGCAGCC TCTTGTGGTT
    GGCAGTAGCA TTGCTGTTGG ACAGCAACCA GTGGAACCAC CGACGACCTG TGAAACCCAA
    CCAGTAGTTT CTCAACACAG TGATAGCTCA CAAATTGCCC CTTCCTCTGA GTTGCCTTCC
    ACAAGAGAAA GTGCTGTGAC CTTGTTGGCT ACAGATTCTA GTGGTATCCT TGAAGAACCT
    TTGCCTTCTA CAAGCAGTGA AGAGGAAGAC CCATTAGCAG GCATAAATCT TCCAGAGGGT
    GTGGATCCTT CTTTCCTAGC GGCTCTTCCT GAAGACATCC GCAGAGAGGT GCTGCAGAAT
    CAGTTGGGCA TACGGCCGCC ATCGCGACCT CCACCCACTG CTGCCCCAGT TATTCCTCCT
    CCTGTGGTTG CTAATGCTGG TGTCACGGAG GTCAGCCCTG AGTTCTTGGC AGCGTTGCCA
    CCAGCCATTC AGGAAGAGGT CCTAGCACAG CAGCGAGCAG AACAGCAGAG GCGGGAACTT
    GCTCAGACCA CTACGTCTGA TACACCCATG GATCCTGTGA CTTTCATTCA GACGTTACCT
    TCTGATCTAC GTCGTAGTGT GTTAGAAGAC ATGGAGGACA GTGTTTTAGC TGTGATGCCA
    CCTGACATTG CTGCAGAGGC ACAGGCTCTA AGGCGAGAGC AAGAAGCTCG GCAGAGGCAA
    CTAATGCATG AACGACTGTT CGGGCACAGT AGCACATCTG CACTCTCTGC CATCCTTAGG
    AGTCCTGCTT TCACGAGTCG CTTGAGTGGA AACCGAGGTG TTCAGTACAC ACGGTTGGCT
    GTACAGAGAG GGAACACTTT CCAAATGGGA AGCAGCAGTA CACATACCAG ACCAACCGGT
    AGTAATGTTG ACAGCCTACT ACGATTACGA GGCCGACTAT TACTTGATCA TGAAGCCCTC
    TCCTGTCTGC TTGTCCTACT GTTTGTTGAT GAACCCAAGC TAAACACCAG TCGCTTGCAC
    CGTGTTCTAC GCAACCTCTG CTACCATGCA CAAACAAGGA ACTGGGTGAT TAGAAGCTTG
    TTGTCTATAC TGCAGCGTAG CAGTGAAAGT GAGCTTTGTA TAGAAAGCCC AAAGACTTCT
    ATTGGGGGCA CTGATAAACG GGGTGCAAAA ACAAGTCATG ACAACCGCCC TTTAGAATTT
    CTGCATAAGA TGGAATCGAA GAATTCCAAC CAGCTCTCCT GGCTTTCTGT ATCCATGGAC
    GCTGCTCTTG GCTGCCGTAC GAACATCTTC CAAATCCAGA GGTCCCTAGG ACGCAAGCAT
    ACAGAAAAGC ATTCAGCCTG TGGTTCTACT GTCCACATCC ATCCCCAAGC AGCCCCTGTA
    GTGTGTAGAC ATGTGCTTGA CACACTAATA CAGCTAGCTA AGGTGTTTCC CAGTCATTTC
    ACACAGCAGC GAACCAAAGA ATGTAGTGTG GAAAACAGTG ACAGCAGGGA GCGGAGTATA
    AAACTTACTT GTAGTCCGTG TCTTGCACCA TCTAGTAGTA TTGGAGCAAG CAACATCTCC
    ACAGATTTCT GGGACTTGCT AGTCAAGCTA GACAACATGA ATGTGAGCCG GAAGGGGAAA
    ACGTCTATAA AATCTGTGCC AGTGAGCTCT GGTTCTGAAG GCGAGAGCAA TCAGTGCAGC
    CTGGAGGCCT CCCCCTTAGG ACAGCTTATG AATATGCTGT CTCATTCTGT TATTCGCCGC
    AGTTCGCTGC TTACTGAAAA GCTGCTGAGA CTTCTCTCCC TTATCTCTAT TGCCCTTCCT
    GATAATAAGG CTTCTGAAAC AGCAGGCAAT CAGAGCAATT CTATTACCCC AGCACCTGCA
    CCTACGCCTG CAACTGCACC AGCCCCTGTG CCTGTACCTG CTGTAACAGT TCCAGTTGTT
    GTGAGCACAA ACATAGTGAC AACTGTTACA ACCAAAAGTG GAAAATCTCC TGCAAAATCA
    AGTGAAGTCA GCAATGCACC AGATAAGATG GCAGCTTCTG GCCTTACAGA GAAACAGCTG
    CAGTTGTCTG TTGAGGTTCT GACATCACAC TCGTGTTCAG AAGAAGGACT GGAGGATGCC
    GCCAATGTTC TACTTCAGCT TTCCAGAGGC GACCCCAGTA CACGGGACAC TGTACTGAAA
    CTTCTGCTTA GCGGAGCACA TCACTTGGGG TTTACTCTCT GCAAACAGAT AGGTACACTA
    CTAGCAGAGC TCCGGGAATA TAACCTAGAG CAGCAGCGTC GTGCACAGTG TGAAGTTCTT
    TCTCCAGATG GTATGCCTGA GGAGCAACCC CACAGCACAA AGCCAAAAGG CAAAATGCAG
    AGCAGTGTTA GGTCTCTCCC CACCCCACCT ATGCGATCCA GGTTCGACAT TGCAGAGAAT
    GTGGTGATTG TGGCTTCCCA AAAGCGTCCA CTGGGAGGAA GAGAGCTGCA GCTCCCCTCA
    ATGTCTATGC TGACGTCAAA GACTTCCACA CAAAAATTCT TTCTTCGAGT GCTGCAGGTT
    ATCATCCAGC TTCGCGATGA CACTCGCAGA GCCAACAAGA AGGCCAAGCA GACCAGCAGA
    TTAGGCACTG CCAGTCTGGC CTCGGCTAGC AGCATCCAAG CAGCTGTGCA GCAGCTGGAG
    GCTGAGGCTG ATGCCATTAT ACAAATGGCG GAGGGTTCTG CTCGCAGGGA GGAGCAGCCT
    ATGGATGTTG ACCAGCCTTC GCCTGCTACA CAGGAGCCTC AGTCAGTTGC TTCTGAAGGT
    TCTCAGGCTG CTGAAAAAAA AGAAGAGGAG AGGTTACCAG AGTTACCGCT GTTAAGTGAG
    CAGCTAAGTT TAGATGAGCT GTGGGACATG TTGGGAGAGT GCCTGAAGGA ACTTGAGGAA
    TCTCATGACC AGCATGCCGT ATTAGTGTTA CAGCCAGCCG TAGAAGCCTT TTTCCTTGTT
    CATGCAACAG AGCGGGAGAG CAAGCCTCCT GTGCGGGACA CTCGGGAAAG CCAGTTATCT
    CACATTAAGG ATGAACCACC TCCCCTGTCT CCTGCACCCC TCACTCCAGC CACACCTTCT
    TCCTTGGATC CTTTCTTCTC TCGTGAACCT TCATCTATGC ACATTTCCTC TAGTTTGCCT
    CCTGACACTC AGAAGTTCCT AAGATTTGCT GAGACTCATC GGACTGTATT AAATCAGATC
    CTTCGCCAGT CCACCACACA CCTAGCTGAT GGGCCCTTTG CTGTGCTTGT AGACTATATC
    AGAGTCCTTG ACTTTGATGT TAAGCGCAAA TATTTCCGAC AGGAGTTGGA GCGACTGGAT
    GAAGGGCTAA GAAAAGAGGA TATGGCAGTG CATGTGCGGC GAGATCATGT GTTTGAGGAT
    TCCTACCGGG AGCTGCACCG CAAATCCCCT GAAGAAATGA AGAATAGATT GTACATAGTA
    TTTGAAGGAG AAGAAGGGCA GGATGCAGGT GGGCTGCTTC GGGAGTGGTA CATGATTATC
    TCCAGGGAGA TGTTCAATCC CATGTATGCT TTGTTCCGCA CTTCCCCAGG TGACCGCGTC
    ACCTACACTA TTAATCCTTC CTCCCACTGT AACCCTAACC ACCTCAGCTA CTTCAAGTTT
    GTGGGCCGGA TAGTTGCCAA AGCGGTTTAT GACAACCGGC TACTGGAGTG TTACTTCACT
    AGGTCCTTCT ACAAGCATAT ATTGGGCAAG TCTGTCAGAT ACACAGACAT GGAGAGTGAA
    GATTATCACT TCTATCAGGG TCTGGTGTAC TTGCTTGAAA ATGATGTCTC CACACTGGGA
    TATGACTTGA CTTTCAGCAC TGAGGTTCAA GAATTTGGTG TCTGTGAAGT CCGAGACCTA
    AAACCAAACG GTGCAAACAT TCTGGTAACA GAAGAAAACA AAAAGGAGTA TGTTCATCTT
    GTGTGCCAGA TGAAGATGAC AGGTGCAATA CGTAAGCAAT TGGCTGCCTT CCTTGAGGGA
    TTTTATGAGA TAATCCCCAA ACGTCTGATC TCCATCTTCA CTGAGCAGGA ATTGGAACTG
    CTTATCTCTG GCCTTCCCAC CATAGATATT GATGACCTAA AATCAAACAC TGAGTATCAC
    AAATACCAGT CTAACTCCAT CCAGATTCAG TGGTTCTGGA GAGCGTTGCG CTCATTCGAT
    CAAGCTGACC GGGCAAAGTT CCTTCAGTTT GTTACTGGAA CTTCAAAGGT CCCATTACAG
    GGTTTTGCTG CGCTGGAAGG AATGAACGGC ATCCAGAAAT TCCAAATCCA CAGAGATGAT
    AGATCTACAG ACAGGCTGCC TTCTGCGCAC ACGTGCTTCA ACCAGTTGGA CCTTCCAGCC
    TATGAGAGTT ACGAGAAGCT ACGTCACATG CTGCTGTTAG CCATCCAGGA GTGCTCTGAG
    GGCTTTGGCC TTGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748035.1
    CDS671..13768
    Translation

    Target ORF information:

    RefSeq Version XM_031892175.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892175.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGGTGCTG ATGGATATGG GTTTCACAAG GGAGCATGCT
    CTGGAAGCTC TGCTTAACAC GAGTACTATG GAGCAGGCCA CTGAATATCT CCTCACCCAC
    CCTCCACCTC TTATTGGCGG AGTTGTACGG GACTTAAGTA TGTCAGAGGA AGATCAGATG
    CTGAGAGCCA TTGCCATGTC GCTGGGACAA GATATCCCTA TGGAACAGAG GGCAGAACTG
    CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG GAGGAACGCA AGGCACGAGA AAAACAAGAG
    GAAGAGGAAG CTAAATGCTT GGAAAAGTTT CAAGATGCAG AACCCCTGGA ACAGGATGAG
    CTTCAAGGAT TCACAGACAC CATGCTCCCT GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA
    GACACAGTCT ATAGAGTGTG TGATCTTATA ATGACTGCCA TAAAGCGCAA TGGGGCAGAC
    TATCGAGACA TGATTCTAAA ACAAGTGGTT AACCAGGTAT GGGAAGCCGC TGATGTTCTT
    ATTAAAGCTG CACTGCCGCT GACCACAAGT GACACAAAAA CTGTGTCTGA GTGGATCAGC
    CAAATGGCTA CTCTTCCTCA AGCTTCTAAC TTGGCCACTC GCATCCTTTT GCTCACACTG
    CTGTTTGAGG AGCTAAAACT GCCATGTGCT AGAGTTGTTG AAACAAGTGG AATACTTAAC
    GTACTAATTA AGCTGCTTGA AGTGGTTCAG CCATGTTTGC AGGCAGCCAA GGAGCAAAAG
    GAAGTTCAGA CACCAAAGTG GATTACACCA GTCCTCCTCT TAATAGATTT TTATGAGAAG
    ACCTCAATTT CTTCTAAAAG AAGAATGCAA ATGAACAGGT ACCTTCAAGC TAATGGAAAC
    AACTGGCGAT GGTTTGATGA TCGGTCTGGC CGTTGGTGCA GCTACAGTGC TAGTAACAAC
    AGCACTATTG ATGCAGCCTG GAAAGCAGGA GAAACATGTG TGCGATTTAC AGCTGGCCGT
    CGAAGATATA CTGTGCAGTT TACCACAATG GTTCAGGTAA ATGAAGAGAC CGGCAATAGA
    CGACCTGTCA TGCTGACTCT CTTGCGTGTT CCGAGACTTT TAAAGAGTGG AAAAAATGGA
    AATGGACAGG AACTTGAAAA GACACTTGAG GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA
    GACGAAAAAG TGCCTGAGCC CATTCCAGTT CCTGAAACCT CTTCCACTGA GAAAGATGCA
    CCAACTGAAG AGCCCAAAGT TGGAGACATA GTAATTCAGG GTCTGGCTGA AGAAATGGTG
    ACCGTCCTTA TACGTTCCTG CGTCGGCATG CTTGGTGTTC CTGTTGATCC AGATACTCTT
    CATGCAACCT TGCGTCTTTG CCTGCGTCTC ACACGTGACC ACAAATATGC CATGATGTTT
    GCAGAGCTTA AGAGCACTCG AATGATCCTC AGCTTAACTC AAAGCTCTGG ATTCAATGGC
    TTTACTCCCC TTGTGACGCT CCTATTTAGG CACATCATAG AGGATCCTTG CACCCTCCAG
    CACACCATGG AAAAGGTTGT ACGGTCTGCA GCAACTAGTG GGGCAGGGAG TACCACATCT
    GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT GAAATCAACT ATATACTTAG AGTACTGGGT
    CCAGCAGCTT GTCGAAACCC TGACATTTTC ACTGAAGTGG CAAACAGTTG CATTCGCATT
    GCTCTCCCTG CACCTCGTGG ATCTGGAACT GCCTCTGATG ATGAGTTTGA GAACCTTCGG
    ATCAAAGGTC CTAATGCAGT TCAGTTGGTT AAAACCACCC CTATAAAGCC CTCCACCTTG
    CCAGTCATCC CTGATACAAT TAAGGAAGTA ATCTATGACA TGCTGAATGC ACTGGCAGCT
    TACCACGCCC CTGAGGAAGT ATCTGAAAAG TCTGAGCAAA AACCTGTTGG CATGAACCAG
    GAAGTCAGCC AGATCCTACA GGACATGGGT GATGAGGTTT TCCAGCAATA TCGGGACTTA
    ACCAGACAGG GAAGCGACTT TGATGGTCAG ACCAGTTTTG CCATCAACGC GCGAGTCTTT
    ACTGCTGACA GCACAGCTGC CGAAACACCT CAGTCTGGTA CACCTCAAGG TGAAACTGCC
    TTAACACCTG AAGAAAACCG AGAAGGGTTA AAGAAAGATA AGGAAGGAGA CCGTAGTTCA
    GAAGATGGAA AGCAGAAGGC AAAAGGTAGT AAGCCTTTGC TGCCTACCTC TTCCATTCTT
    CGACTACTTG CTGAACTTGT CAGGTCCTAT GTCGGAATAG CCTCGCTGAT CGCCACCTAC
    AATTACACTG CAGGACAATC TGAACTAATT AAAGAGGATT GCAGTGTATT GGCGTTTGTG
    CTGGATCACC TGCTACCCCA CACACATAAT GCAGAGGACA AGGACACACC AGCCCTAGCC
    CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA GGAAGTGGCA CTGATGCCCA GGTTGCACTA
    GTGAATGAAG TAAAGGCAGC TCTTGGCAGA GCCCTAGCAA TGGCTGAGAG CACGGAGAAG
    CATGCTAGGT TGCAGGCTGT GATGTGCATT ATCAGTACCA TTATGGAGTC ATGTCCATCC
    ACCTCCAGCT TCTACAGCAC AGCAACCACC AAAACCCAGC ACAATGGCAT GAACAACATC
    ATCAGACTCT TTTTGAAAAA GGGGCTGGTT AGTGATTTAG CCAGGGTCCC TCACAGCCTT
    GATTTATCCA GTCCAAATAT GGCAAACACC GTGAATGCAG CACTGAAGCC ACTCGAGACA
    CTGTCTCGCA TTGTCAATCA ACCCAGCAGT TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG
    AATAAATCTG AGGCAGATAC ACATGGAACA GCCCAGGATC CCAACAGTAA TCAGCCAGAT
    ACAGGTGAAA CAGGAACAGA AGTGCAGGAA GAGGACCCAG ATGCTTCTCA TGCTGAAGTA
    GCTGATGGAG ACATCATGGA TGGAGAGACA GAGACTGATG CTGTGGTTAT TGCTGGCCAG
    CCTGAAGTCC TTACTACACA AGAAATGCAG GTAGAGAATG AGCTTGAAGA TCTGATAGAT
    GAACTTCTTG AAAGGGATGG TGCTGCAGGT AACCGGGTCG GAGAAGATGA ATCACAGGAA
    GATGTGCTGA TGGATGAGCC TCCTACTAAC CTCAGCCAAG CCTCCACTCT TCAGGCTAAT
    CGAGAAGACT CTATGAACAT CTTAGACCCA GAGGATGAGG AAGAGCACAC CCAAGAAGAG
    GACAGCAGTG GCAGCAATGA AGATGAAGAT GACAGCCAGG AAGAGGAGGA GGAAGAGGAA
    GATGAGGACG ATCAGGATGA TGAGGAGGGT GAGGAAGGGG ATGAGGATGA TGATGATGAC
    TGTTCAGAGA TGGAACTGGA TGAGGATTAT CCAGACATGA ATGCCTCACC TCTTGTACGC
    TTTGAACGTT TTGACAGAGA AGATGATTTA ATCATAGAGT TTGATAACAT GTTCTCCAGT
    GCCACTGAGA TCCCTCCATC CCCTGGTAAC ATCCCTACTA CACACCCCCT AATGGTGCGA
    CACGCTGATC ACGGCTCCTT GACTCTTGGC AGTGGCACTT CCACTACTCG CCTGGCACAG
    GGCATCGGGC GCAGCCAGAG AACACTCCGA CAACTCACAG CCAACACCGG TCACACAATA
    CATGTTCATT ATCCTGGCAA CAGGCAGCCA AATCCACCTT TAATCCTCCA GAGGTTGCTG
    GGGCCCTCAG CCGCTGCTGA CATCTTGCAA CTAAGCAGCA GTCTTCCTCT GCAGAGTCGT
    GGCCGAGCAC GTCTACTTGT TGGCAACGAT GATGTCCACA TCATAGCACG CTCTGATGAT
    GAGATGCTTG ATGATTTTTT CCATGATCAG AGTACAGCCA CAAGTCAGGC TGGCACACTT
    TCCAGCATTC CAACTGCGCT GACCCGCTGG ACAGAAGAGT GTAAGGTCTT GGATGCTGAA
    AGCATGCATG ACTGTGTATC TGTGGTCAAA GTCCCAATCA TCAATCATTT GGAATCTTTG
    CGTGATGAGG AGCTTGAGGA GCGCAGGGAA AAAAGAAGGA AACAACTAGC AGAAGAGGAG
    GCCAAGATGG CAGAGAAATC CAAGGAGGAG AAGGAAGCAA AGGAGCAAAA TTCACAGGGT
    GCCAATTCAA AGCAGAATAC CCCATTTGAA CTGAGCCTTG CAGATCAAGA TGGAAGTGGT
    CGTCCTGAAA ATTTCCCAAC TGCACCATCT TCTGCAGAAG GAACTCCAAC AAGTCCTGAA
    ATTGTTCGTT TAGATCCCAC CCTAAGGGCT ACTGAGGCTA CTCTGGCAGA GCTGACTCAG
    GGTCTGCCTG CTGATACAGG CACGGAGGAT GTTGGGCAAC AACTCATGCC ATCTCACCAA
    GAGGACTCTG GACCAGGACG GCCAAGTGTA GAGGGGGATG CCACACAAAT GGAGCTATCG
    CCAGCACCCA CTATAACCTC CTTATCACCA GAGCGAGCAG AGGATTCAGA TGCTTTGACA
    GCTGTTAGTA GCCAACTAGA GGGCTCGCCT ATGGACACCA GCAGTCTTGC CTCTGGGCCC
    TTAGAGGATG GCGTAGGGGA GGCACCTGGA ATTGTTAATC CAGAGCAGCC TCTTGTGGTT
    GGCAGTAGCA TTGCTGTTGG ACAGCAACCA GTGGAACCAC CGACGACCTG TGAAACCCAA
    CCAGTAGTTT CTCAACACAG TGATAGCTCA CAAATTGCCC CTTCCTCTGA GTTGCCTTCC
    ACAAGAGAAA GTGCTGTGAC CTTGTTGGCT ACAGATTCTA GTGGTATCCT TGAAGAACCT
    TTGCCTTCTA CAAGCAGTGA AGAGGAAGAC CCATTAGCAG GCATAAATCT TCCAGAGGGT
    GTGGATCCTT CTTTCCTAGC GGCTCTTCCT GAAGACATCC GCAGAGAGGT GCTGCAGAAT
    CAGTTGGGCA TACGGCCGCC ATCGCGACCT CCACCCACTG CTGCCCCAGT TATTCCTCCT
    CCTGTGGTTG CTAATGCTGG TGTCACGGAG GTCAGCCCTG AGTTCTTGGC AGCGTTGCCA
    CCAGCCATTC AGGAAGAGGT CCTAGCACAG CAGCGAGCAG AACAGCAGAG GCGGGAACTT
    GCTCAGACCA CTACGTCTGA TACACCCATG GATCCTGTGA CTTTCATTCA GACGTTACCT
    TCTGATCTAC GTCGTAGTGT GTTAGAAGAC ATGGAGGACA GTGTTTTAGC TGTGATGCCA
    CCTGACATTG CTGCAGAGGC ACAGGCTCTA AGGCGAGAGC AAGAAGCTCG GCAGAGGCAA
    CTAATGCATG AACGACTGTT CGGGCACAGT AGCACATCTG CACTCTCTGC CATCCTTAGG
    AGTCCTGCTT TCACGAGTCG CTTGAGTGGA AACCGAGGTG TTCAGTACAC ACGGTTGGCT
    GTACAGAGAG GGAACACTTT CCAAATGGGA AGCAGCAGTA CACATACCAG ACCAACCGGT
    AGTAATGTTG ACAGCCTACT ACGATTACGA GGCCGACTAT TACTTGATCA TGAAGCCCTC
    TCCTGTCTGC TTGTCCTACT GTTTGTTGAT GAACCCAAGC TAAACACCAG TCGCTTGCAC
    CGTGTTCTAC GCAACCTCTG CTACCATGCA CAAACAAGGA ACTGGGTGAT TAGAAGCTTG
    TTGTCTATAC TGCAGCGTAG CAGTGAAAGT GAGCTTTGTA TAGAAAGCCC AAAGACTTCT
    ATTGGGGGCA CTGATAAACG GGGTGCAAAA ACAAGTCATG ACAACCGCCC TTTAGAATTT
    CTGCATAAGA TGGAATCGAA GAATTCCAAC CAGCTCTCCT GGCTTTCTGT ATCCATGGAC
    GCTGCTCTTG GCTGCCGTAC GAACATCTTC CAAATCCAGA GGTCCCTAGG ACGCAAGCAT
    ACAGAAAAGC ATTCAGCCTG TGGTTCTACT GTCCACATCC ATCCCCAAGC AGCCCCTGTA
    GTGTGTAGAC ATGTGCTTGA CACACTAATA CAGCTAGCTA AGGTGTTTCC CAGTCATTTC
    ACACAGCAGC GAACCAAAGA ATGTAGTGTG GAAAACAGTG ACAGCAGGGA GCGGAGTATA
    AAACTTACTT GTAGTCCGTG TCTTGCACCA TCTAGTAGTA TTGGAGCAAG CAACATCTCC
    ACAGATTTCT GGGACTTGCT AGTCAAGCTA GACAACATGA ATGTGAGCCG GAAGGGGAAA
    ACGTCTATAA AATCTGTGCC AGTGAGCTCT GGTTCTGAAG GCGAGAGCAA TCAGTGCAGC
    CTGGAGGCCT CCCCCTTAGG ACAGCTTATG AATATGCTGT CTCATTCTGT TATTCGCCGC
    AGTTCGCTGC TTACTGAAAA GCTGCTGAGA CTTCTCTCCC TTATCTCTAT TGCCCTTCCT
    GATAATAAGG CTTCTGAAAC AGCAGGCAAT CAGAGCAATT CTATTACCCC AGCACCTGCA
    CCTACGCCTG CAACTGCACC AGCCCCTGTG CCTGTACCTG CTGTAACAGT TCCAGTTGTT
    GTGAGCACAA ACATAGTGAC AACTGTTACA ACCAAAAGTG GAAAATCTCC TGCAAAATCA
    AGTGAAGTCA GCAATGCACC AGATAAGATG GCAGCTTCTG GCCTTACAGA GAAACAGCTG
    CAGTTGTCTG TTGAGGTTCT GACATCACAC TCGTGTTCAG AAGAAGGACT GGAGGATGCC
    GCCAATGTTC TACTTCAGCT TTCCAGAGGC GACCCCAGTA CACGGGACAC TGTACTGAAA
    CTTCTGCTTA GCGGAGCACA TCACTTGGGG TTTACTCTCT GCAAACAGAT AGGTACACTA
    CTAGCAGAGC TCCGGGAATA TAACCTAGAG CAGCAGCGTC GTGCACAGTG TGAAGTTCTT
    TCTCCAGATG GTATGCCTGA GGAGCAACCC CACAGCACAA AGCCAAAAGG CAAAATGCAG
    AGCAGTGTTA GGTCTCTCCC CACCCCACCT ATGCGATCCA GGTTCGACAT TGCAGAGAAT
    GTGGTGATTG TGGCTTCCCA AAAGCGTCCA CTGGGAGGAA GAGAGCTGCA GCTCCCCTCA
    ATGTCTATGC TGACGTCAAA GACTTCCACA CAAAAATTCT TTCTTCGAGT GCTGCAGGTT
    ATCATCCAGC TTCGCGATGA CACTCGCAGA GCCAACAAGA AGGCCAAGCA GACCAGCAGA
    TTAGGCACTG CCAGTCTGGC CTCGGCTAGC AGCATCCAAG CAGCTGTGCA GCAGCTGGAG
    GCTGAGGCTG ATGCCATTAT ACAAATGGCG GAGGGTTCTG CTCGCAGGGA GGAGCAGCCT
    ATGGATGTTG ACCAGCCTTC GCCTGCTACA CAGGAGCCTC AGTCAGTTGC TTCTGAAGGT
    TCTCAGGCTG CTGAAAAAAA AGAAGAGGAG AGGTTACCAG AGTTACCGCT GTTAAGTGAG
    CAGCTAAGTT TAGATGAGCT GTGGGACATG TTGGGAGAGT GCCTGAAGGA ACTTGAGGAA
    TCTCATGACC AGCATGCCGT ATTAGTGTTA CAGCCAGCCG TAGAAGCCTT TTTCCTTGTT
    CATGCAACAG AGCGGGAGAG CAAGCCTCCT GTGCGGGACA CTCGGGAAAG CCAGTTATCT
    CACATTAAGG ATGAACCACC TCCCCTGTCT CCTGCACCCC TCACTCCAGC CACACCTTCT
    TCCTTGGATC CTTTCTTCTC TCGTGAACCT TCATCTATGC ACATTTCCTC TAGTTTGCCT
    CCTGACACTC AGAAGTTCCT AAGATTTGCT GAGACTCATC GGACTGTATT AAATCAGATC
    CTTCGCCAGT CCACCACACA CCTAGCTGAT GGGCCCTTTG CTGTGCTTGT AGACTATATC
    AGAGTCCTTG ACTTTGATGT TAAGCGCAAA TATTTCCGAC AGGAGTTGGA GCGACTGGAT
    GAAGGGCTAA GAAAAGAGGA TATGGCAGTG CATGTGCGGC GAGATCATGT GTTTGAGGAT
    TCCTACCGGG AGCTGCACCG CAAATCCCCT GAAGAAATGA AGAATAGATT GTACATAGTA
    TTTGAAGGAG AAGAAGGGCA GGATGCAGGT GGGCTGCTTC GGGAGTGGTA CATGATTATC
    TCCAGGGAGA TGTTCAATCC CATGTATGCT TTGTTCCGCA CTTCCCCAGG TGACCGCGTC
    ACCTACACTA TTAATCCTTC CTCCCACTGT AACCCTAACC ACCTCAGCTA CTTCAAGTTT
    GTGGGCCGGA TAGTTGCCAA AGCGGTTTAT GACAACCGGC TACTGGAGTG TTACTTCACT
    AGGTCCTTCT ACAAGCATAT ATTGGGCAAG TCTGTCAGAT ACACAGACAT GGAGAGTGAA
    GATTATCACT TCTATCAGGG TCTGGTGTAC TTGCTTGAAA ATGATGTCTC CACACTGGGA
    TATGACTTGA CTTTCAGCAC TGAGGTTCAA GAATTTGGTG TCTGTGAAGT CCGAGACCTA
    AAACCAAACG GTGCAAACAT TCTGGTAACA GAAGAAAACA AAAAGGAGTA TGTTCATCTT
    GTGTGCCAGA TGAAGATGAC AGGTGCAATA CGTAAGCAAT TGGCTGCCTT CCTTGAGGGA
    TTTTATGAGA TAATCCCCAA ACGTCTGATC TCCATCTTCA CTGAGCAGGA ATTGGAACTG
    CTTATCTCTG GCCTTCCCAC CATAGATATT GATGACCTAA AATCAAACAC TGAGTATCAC
    AAATACCAGT CTAACTCCAT CCAGATTCAG TGGTTCTGGA GAGCGTTGCG CTCATTCGAT
    CAAGCTGACC GGGCAAAGTT CCTTCAGTTT GTTACTGGAA CTTCAAAGGT CCCATTACAG
    GGTTTTGCTG CGCTGGAAGG AATGAACGGC ATCCAGAAAT TCCAAATCCA CAGAGATGAT
    AGATCTACAG ACAGGCTGCC TTCTGCGCAC ACGTGCTTCA ACCAGTTGGA CCTTCCAGCC
    TATGAGAGTT ACGAGAAGCT ACGTCACATG CTGCTGTTAG CCATCCAGGA GTGCTCTGAG
    GGCTTTGGCC TTGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65596
    Clone ID Related Accession (Same CDS sequence) XM_031892176.1
    Accession Version XM_031892176.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1321
    1381
    1441
    1501
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    1981
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    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGGTGCTG ATGGATATGG GTTTCACAAG GGAGCATGCT
    CTGGAAGCTC TGCTTAACAC GAGTACTATG GAGCAGGCCA CTGAATATCT CCTCACCCAC
    CCTCCACCTC TTATTGGCGG AGTTGTACGG GAAACTGCTC GGCGGAAAGA AGAGGAGGAA
    CGCAAGGCAC GAGAAAAACA AGAGGAAGAG GAAGCTAAAT GCTTGGAAAA GTTTCAAGAT
    GCAGAACCCC TGGAACAGGA TGAGCTTCAA GGATTCACAG ACACCATGCT CCCTGGCTGC
    TTCCACTTGC TTGATGAGCT GCCAGACACA GTCTATAGAG TGTGTGATCT TATAATGACT
    GCCATAAAGC GCAATGGGGC AGACTATCGA GACATGATTC TAAAACAAGT GGTTAACCAG
    GTATGGGAAG CCGCTGATGT TCTTATTAAA GCTGCACTGC CGCTGACCAC AAGTGACACA
    AAAACTGTGT CTGAGTGGAT CAGCCAAATG GCTACTCTTC CTCAAGCTTC TAACTTGGCC
    ACTCGCATCC TTTTGCTCAC ACTGCTGTTT GAGGAGCTAA AACTGCCATG TGCTAGAGTT
    GTTGAAACAA GTGGAATACT TAACGTACTA ATTAAGCTGC TTGAAGTGGT TCAGCCATGT
    TTGCAGGCAG CCAAGGAGCA AAAGGAAGTT CAGACACCAA AGTGGATTAC ACCAGTCCTC
    CTCTTAATAG ATTTTTATGA GAAGACCTCA ATTTCTTCTA AAAGAAGAAT GCAAATGAAC
    AGGTACCTTC AAGCTAATGG AAACAACTGG CGATGGTTTG ATGATCGGTC TGGCCGTTGG
    TGCAGCTACA GTGCTAGTAA CAACAGCACT ATTGATGCAG CCTGGAAAGC AGGAGAAACA
    TGTGTGCGAT TTACAGCTGG CCGTCGAAGA TATACTGTGC AGTTTACCAC AATGGTTCAG
    GTAAATGAAG AGACCGGCAA TAGACGACCT GTCATGCTGA CTCTCTTGCG TGTTCCGAGA
    CTTTTAAAGA GTGGAAAAAA TGGAAATGGA CAGGAACTTG AAAAGACACT TGAGGAGGCA
    AAGGAGGTTG AAGCAAAGCA AAAAGACGAA AAAGTGCCTG AGCCCATTCC AGTTCCTGAA
    ACCTCTTCCA CTGAGAAAGA TGCACCAACT GAAGAGCCCA AAGTTGGAGA CATAGTAATT
    CAGGGTCTGG CTGAAGAAAT GGTGACCGTC CTTATACGTT CCTGCGTCGG CATGCTTGGT
    GTTCCTGTTG ATCCAGATAC TCTTCATGCA ACCTTGCGTC TTTGCCTGCG TCTCACACGT
    GACCACAAAT ATGCCATGAT GTTTGCAGAG CTTAAGAGCA CTCGAATGAT CCTCAGCTTA
    ACTCAAAGCT CTGGATTCAA TGGCTTTACT CCCCTTGTGA CGCTCCTATT TAGGCACATC
    ATAGAGGATC CTTGCACCCT CCAGCACACC ATGGAAAAGG TTGTACGGTC TGCAGCAACT
    AGTGGGGCAG GGAGTACCAC ATCTGGGGTT GTGTCTGGAA GCCTGGGTTC GCGTGAAATC
    AACTATATAC TTAGAGTACT GGGTCCAGCA GCTTGTCGAA ACCCTGACAT TTTCACTGAA
    GTGGCAAACA GTTGCATTCG CATTGCTCTC CCTGCACCTC GTGGATCTGG AACTGCCTCT
    GATGATGAGT TTGAGAACCT TCGGATCAAA GGTCCTAATG CAGTTCAGTT GGTTAAAACC
    ACCCCTATAA AGCCCTCCAC CTTGCCAGTC ATCCCTGATA CAATTAAGGA AGTAATCTAT
    GACATGCTGA ATGCACTGGC AGCTTACCAC GCCCCTGAGG AAGTATCTGA AAAGTCTGAG
    CAAAAACCTG TTGGCATGAA CCAGGAAGTC AGCCAGATCC TACAGGACAT GGGTGATGAG
    GTTTTCCAGC AATATCGGGA CTTAACCAGA CAGGGAAGCG ACTTTGATGG TCAGACCAGT
    TTTGCCATCA ACGCGCGAGT CTTTACTGCT GACAGCACAG CTGCCGAAAC ACCTCAGTCT
    GGTACACCTC AAGGTGAAAC TGCCTTAACA CCTGAAGAAA ACCGAGAAGG GTTAAAGAAA
    GATAAGGAAG GAGACCGTAG TTCAGAAGAT GGAAAGCAGA AGGCAAAAGG TAGTAAGCCT
    TTGCTGCCTA CCTCTTCCAT TCTTCGACTA CTTGCTGAAC TTGTCAGGTC CTATGTCGGA
    ATAGCCTCGC TGATCGCCAC CTACAATTAC ACTGCAGGAC AATCTGAACT AATTAAAGAG
    GATTGCAGTG TATTGGCGTT TGTGCTGGAT CACCTGCTAC CCCACACACA TAATGCAGAG
    GACAAGGACA CACCAGCCCT AGCCCGCCTC TTCTTAGCCA GCCTTGCTGC AGCAGGAAGT
    GGCACTGATG CCCAGGTTGC ACTAGTGAAT GAAGTAAAGG CAGCTCTTGG CAGAGCCCTA
    GCAATGGCTG AGAGCACGGA GAAGCATGCT AGGTTGCAGG CTGTGATGTG CATTATCAGT
    ACCATTATGG AGTCATGTCC ATCCACCTCC AGCTTCTACA GCACAGCAAC CACCAAAACC
    CAGCACAATG GCATGAACAA CATCATCAGA CTCTTTTTGA AAAAGGGGCT GGTTAGTGAT
    TTAGCCAGGG TCCCTCACAG CCTTGATTTA TCCAGTCCAA ATATGGCAAA CACCGTGAAT
    GCAGCACTGA AGCCACTCGA GACACTGTCT CGCATTGTCA ATCAACCCAG CAGTTTGTTT
    GGAAGCAAAG GGACTTCAGG AAAGAATAAA TCTGAGGCAG ATACACATGG AACAGCCCAG
    GATCCCAACA GTAATCAGCC AGATACAGGT GAAACAGGAA CAGAAGTGCA GGAAGAGGAC
    CCAGATGCTT CTCATGCTGA AGTAGCTGAT GGAGACATCA TGGATGGAGA GACAGAGACT
    GATGCTGTGG TTATTGCTGG CCAGCCTGAA GTCCTTACTA CACAAGAAAT GCAGGTAGAG
    AATGAGCTTG AAGATCTGAT AGATGAACTT CTTGAAAGGG ATGGTGCTGC AGGTAACCGG
    GTCGGAGAAG ATGAATCACA GGAAGATGTG CTGATGGATG AGCCTCCTAC TAACCTCAGC
    CAAGCCTCCA CTCTTCAGGC TAATCGAGAA GACTCTATGA ACATCTTAGA CCCAGAGGAT
    GAGGAAGAGC ACACCCAAGA AGAGGACAGC AGTGGCAGCA ATGAAGATGA AGATGACAGC
    CAGGAAGAGG AGGAGGAAGA GGAAGATGAG GACGATCAGG ATGATGAGGA GGGTGAGGAA
    GGGGATGAGG ATGATGATGA TGACTGTTCA GAGATGGAAC TGGATGAGGA TTATCCAGAC
    ATGAATGCCT CACCTCTTGT ACGCTTTGAA CGTTTTGACA GAGAAGATGA TTTAATCATA
    GAGTTTGATA ACATGTTCTC CAGTGCCACT GAGATCCCTC CATCCCCTGG TAACATCCCT
    ACTACACACC CCCTAATGGT GCGACACGCT GATCACGGCT CCTTGACTCT TGGCAGTGGC
    ACTTCCACTA CTCGCCTGGC ACAGGGCATC GGGCGCAGCC AGAGAACACT CCGACAACTC
    ACAGCCAACA CCGGTCACAC AATACATGTT CATTATCCTG GCAACAGGCA GCCAAATCCA
    CCTTTAATCC TCCAGAGGTT GCTGGGGCCC TCAGCCGCTG CTGACATCTT GCAACTAAGC
    AGCAGTCTTC CTCTGCAGAG TCGTGGCCGA GCACGTCTAC TTGTTGGCAA CGATGATGTC
    CACATCATAG CACGCTCTGA TGATGAGATG CTTGATGATT TTTTCCATGA TCAGAGTACA
    GCCACAAGTC AGGCTGGCAC ACTTTCCAGC ATTCCAACTG CGCTGACCCG CTGGACAGAA
    GAGTGTAAGG TCTTGGATGC TGAAAGCATG CATGACTGTG TATCTGTGGT CAAAGTCCCA
    ATCATCAATC ATTTGGAATC TTTGCGTGAT GAGGAGCTTG AGGAGCGCAG GGAAAAAAGA
    AGGAAACAAC TAGCAGAAGA GGAGGCCAAG ATGGCAGAGA AATCCAAGGA GGAGAAGGAA
    GCAAAGGAGC AAAATTCACA GGGTGCCAAT TCAAAGCAGA ATACCCCATT TGAACTGAGC
    CTTGCAGATC AAGATGGAAG TGGTCGTCCT GAAAATTTCC CAACTGCACC ATCTTCTGCA
    GAAGGAACTC CAACAAGTCC TGAAATTGTT CGTTTAGATC CCACCCTAAG GGCTACTGAG
    GCTACTCTGG CAGAGCTGAC TCAGGGTCTG CCTGCTGATA CAGGCACGGA GGATGTTGGG
    CAACAACTCA TGCCATCTCA CCAAGAGGAC TCTGGACCAG GACGGCCAAG TGTAGAGGGG
    GATGCCACAC AAATGGAGCT ATCGCCAGCA CCCACTATAA CCTCCTTATC ACCAGAGCGA
    GCAGAGGATT CAGATGCTTT GACAGCTGTT AGTAGCCAAC TAGAGGGCTC GCCTATGGAC
    ACCAGCAGTC TTGCCTCTGG GCCCTTAGAG GATGGCGTAG GGGAGGCACC TGGAATTGTT
    AATCCAGAGC AGCCTCTTGT GGTTGGCAGT AGCATTGCTG TTGGACAGCA ACCAGTGGAA
    CCACCGACGA CCTGTGAAAC CCAACCAGTA GTTTCTCAAC ACAGTGATAG CTCACAAATT
    GCCCCTTCCT CTGAGTTGCC TTCCACAAGA GAAAGTGCTG TGACCTTGTT GGCTACAGAT
    TCTAGTGGTA TCCTTGAAGA ACCTTTGCCT TCTACAAGCA GTGAAGAGGA AGACCCATTA
    GCAGGCATAA ATCTTCCAGA GGGTGTGGAT CCTTCTTTCC TAGCGGCTCT TCCTGAAGAC
    ATCCGCAGAG AGGTGCTGCA GAATCAGTTG GGCATACGGC CGCCATCGCG ACCTCCACCC
    ACTGCTGCCC CAGTTATTCC TCCTCCTGTG GTTGCTAATG CTGGTGTCAC GGAGGTCAGC
    CCTGAGTTCT TGGCAGCGTT GCCACCAGCC ATTCAGGAAG AGGTCCTAGC ACAGCAGCGA
    GCAGAACAGC AGAGGCGGGA ACTTGCTCAG ACCACTACGT CTGATACACC CATGGATCCT
    GTGACTTTCA TTCAGACGTT ACCTTCTGAT CTACGTCGTA GTGTGTTAGA AGACATGGAG
    GACAGTGTTT TAGCTGTGAT GCCACCTGAC ATTGCTGCAG AGGCACAGGC TCTAAGGCGA
    GAGCAAGAAG CTCGGCAGAG GCAACTAATG CATGAACGAC TGTTCGGGCA CAGTAGCACA
    TCTGCACTCT CTGCCATCCT TAGGAGTCCT GCTTTCACGA GTCGCTTGAG TGGAAACCGA
    GGTGTTCAGT ACACACGGTT GGCTGTACAG AGAGGGAACA CTTTCCAAAT GGGAAGCAGC
    AGTACACATA CCAGACCAAC CGGTAGTAAT GTTGACAGCC TACTACGATT ACGAGGCCGA
    CTATTACTTG ATCATGAAGC CCTCTCCTGT CTGCTTGTCC TACTGTTTGT TGATGAACCC
    AAGCTAAACA CCAGTCGCTT GCACCGTGTT CTACGCAACC TCTGCTACCA TGCACAAACA
    AGGAACTGGG TGATTAGAAG CTTGTTGTCT ATACTGCAGC GTAGCAGTGA AAGTGAGCTT
    TGTATAGAAA GCCCAAAGAC TTCTATTGGG GGCACTGATA AACGGGGTGC AAAAACAAGT
    CATGACAACC GCCCTTTAGA ATTTCTGCAT AAGATGGAAT CGAAGAATTC CAACCAGCTC
    TCCTGGCTTT CTGTATCCAT GGACGCTGCT CTTGGCTGCC GTACGAACAT CTTCCAAATC
    CAGAGGTCCC TAGGACGCAA GCATACAGAA AAGCATTCAG CCTGTGGTTC TACTGTCCAC
    ATCCATCCCC AAGCAGCCCC TGTAGTGTGT AGACATGTGC TTGACACACT AATACAGCTA
    GCTAAGGTGT TTCCCAGTCA TTTCACACAG CAGCGAACCA AAGAATGTAG TGTGGAAAAC
    AGTGACAGCA GGGAGCGGAG TATAAAACTT ACTTGTAGTC CGTGTCTTGC ACCATCTAGT
    AGTATTGGAG CAAGCAACAT CTCCACAGAT TTCTGGGACT TGCTAGTCAA GCTAGACAAC
    ATGAATGTGA GCCGGAAGGG GAAAACGTCT ATAAAATCTG TGCCAGTGAG CTCTGGTTCT
    GAAGGCGAGA GCAATCAGTG CAGCCTGGAG GCCTCCCCCT TAGGACAGCT TATGAATATG
    CTGTCTCATT CTGTTATTCG CCGCAGTTCG CTGCTTACTG AAAAGCTGCT GAGACTTCTC
    TCCCTTATCT CTATTGCCCT TCCTGATAAT AAGGCTTCTG AAACAGCAGG CAATCAGAGC
    AATTCTATTA CCCCAGCACC TGCACCTACG CCTGCAACTG CACCAGCCCC TGTGCCTGTA
    CCTGCTGTAA CAGTTCCAGT TGTTGTGAGC ACAAACATAG TGACAACTGT TACAACCAAA
    AGTGGAAAAT CTCCTGCAAA ATCAAGTGAA GTCAGCAATG CACCAGATAA GATGGCAGCT
    TCTGGCCTTA CAGAGAAACA GCTGCAGTTG TCTGTTGAGG TTCTGACATC ACACTCGTGT
    TCAGAAGAAG GACTGGAGGA TGCCGCCAAT GTTCTACTTC AGCTTTCCAG AGGCGACCCC
    AGTACACGGG ACACTGTACT GAAACTTCTG CTTAGCGGAG CACATCACTT GGGGTTTACT
    CTCTGCAAAC AGATAGGTAC ACTACTAGCA GAGCTCCGGG AATATAACCT AGAGCAGCAG
    CGTCGTGCAC AGTGTGAAGT TCTTTCTCCA GATGGTATGC CTGAGGAGCA ACCCCACAGC
    ACAAAGCCAA AAGGCAAAAT GCAGAGCAGT GTTAGGTCTC TCCCCACCCC ACCTATGCGA
    TCCAGGTTCG ACATTGCAGA GAATGTGGTG ATTGTGGCTT CCCAAAAGCG TCCACTGGGA
    GGAAGAGAGC TGCAGCTCCC CTCAATGTCT ATGCTGACGT CAAAGACTTC CACACAAAAA
    TTCTTTCTTC GAGTGCTGCA GGTTATCATC CAGCTTCGCG ATGACACTCG CAGAGCCAAC
    AAGAAGGCCA AGCAGACCAG CAGATTAGGC ACTGCCAGTC TGGCCTCGGC TAGCAGCATC
    CAAGCAGCTG TGCAGCAGCT GGAGGCTGAG GCTGATGCCA TTATACAAAT GGTACGTGAG
    GGTCAAAGGG CGCGGAGACA GCTGCAAACT GCAACGGCGG AGGGTTCTGC TCGCAGGGAG
    GAGCAGCCTA TGGATGTTGA CCAGCCTTCG CCTGCTACAC AGGAGCCTCA GTCAGTTGCT
    TCTGAAGGTT CTCAGGCTGC TGAAAAAAAA GAAGAGGAGA GGTTACCAGA GTTACCGCTG
    TTAAGTGAGC AGCTAAGTTT AGATGAGCTG TGGGACATGT TGGGAGAGTG CCTGAAGGAA
    CTTGAGGAAT CTCATGACCA GCATGCCGTA TTAGTGTTAC AGCCAGCCGT AGAAGCCTTT
    TTCCTTGTTC ATGCAACAGA GCGGGAGAGC AAGCCTCCTG TGCGGGACAC TCGGGAAAGC
    CAGTTATCTC ACATTAAGGA TGAACCACCT CCCCTGTCTC CTGCACCCCT CACTCCAGCC
    ACACCTTCTT CCTTGGATCC TTTCTTCTCT CGTGAACCTT CATCTATGCA CATTTCCTCT
    AGTTTGCCTC CTGACACTCA GAAGTTCCTA AGATTTGCTG AGACTCATCG GACTGTATTA
    AATCAGATCC TTCGCCAGTC CACCACACAC CTAGCTGATG GGCCCTTTGC TGTGCTTGTA
    GACTATATCA GAGTCCTTGA CTTTGATGTT AAGCGCAAAT ATTTCCGACA GGAGTTGGAG
    CGACTGGATG AAGGGCTAAG AAAAGAGGAT ATGGCAGTGC ATGTGCGGCG AGATCATGTG
    TTTGAGGATT CCTACCGGGA GCTGCACCGC AAATCCCCTG AAGAAATGAA GAATAGATTG
    TACATAGTAT TTGAAGGAGA AGAAGGGCAG GATGCAGGTG GGCTGCTTCG GGAGTGGTAC
    ATGATTATCT CCAGGGAGAT GTTCAATCCC ATGTATGCTT TGTTCCGCAC TTCCCCAGGT
    GACCGCGTCA CCTACACTAT TAATCCTTCC TCCCACTGTA ACCCTAACCA CCTCAGCTAC
    TTCAAGTTTG TGGGCCGGAT AGTTGCCAAA GCGGTTTATG ACAACCGGCT ACTGGAGTGT
    TACTTCACTA GGTCCTTCTA CAAGCATATA TTGGGCAAGT CTGTCAGATA CACAGACATG
    GAGAGTGAAG ATTATCACTT CTATCAGGGT CTGGTGTACT TGCTTGAAAA TGATGTCTCC
    ACACTGGGAT ATGACTTGAC TTTCAGCACT GAGGTTCAAG AATTTGGTGT CTGTGAAGTC
    CGAGACCTAA AACCAAACGG TGCAAACATT CTGGTAACAG AAGAAAACAA AAAGGAGTAT
    GTTCATCTTG TGTGCCAGAT GAAGATGACA GGTGCAATAC GTAAGCAATT GGCTGCCTTC
    CTTGAGGGAT TTTATGAGAT AATCCCCAAA CGTCTGATCT CCATCTTCAC TGAGCAGGAA
    TTGGAACTGC TTATCTCTGG CCTTCCCACC ATAGATATTG ATGACCTAAA ATCAAACACT
    GAGTATCACA AATACCAGTC TAACTCCATC CAGATTCAGT GGTTCTGGAG AGCGTTGCGC
    TCATTCGATC AAGCTGACCG GGCAAAGTTC CTTCAGTTTG TTACTGGAAC TTCAAAGGTC
    CCATTACAGG GTTTTGCTGC GCTGGAAGGA ATGAACGGCA TCCAGAAATT CCAAATCCAC
    AGAGATGATA GATCTACAGA CAGGCTGCCT TCTGCGCACA CGTGCTTCAA CCAGTTGGAC
    CTTCCAGCCT ATGAGAGTTA CGAGAAGCTA CGTCACATGC TGCTGTTAGC CATCCAGGAG
    TGCTCTGAGG GCTTTGGCCT TGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748036.1
    CDS671..13717
    Translation

    Target ORF information:

    RefSeq Version XM_031892176.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892176.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGCTTTG ACCTCTCTCC GGTCTCCTGC AGGCGATACA
    GCCTCTAACC TGGGCAGTGC TGTTGATGAA CTGATGAGGC ACCAGCCCAC GTTAAAAACA
    GACGCTACAA CTGCCATCAT TAAGCTCTTA GAAGAGATAT GTAACCTTGG TAGGGACCCA
    AAATACATCT GTCAGAAGCC ATCTATTCAG AAAGCTGATG GCACGGCTAC TGCTCCTCCA
    CCACGTTCCA ACCATGCAGC AGAGGAGGCT TCTAGTGAGG ATGAAGAGGA GGAGGAGGTA
    CAAGCAATGC AGAGTTTCAG CACAAATCAG CAAAGTGAAG TTGAACCCAG CCAGCATGTT
    GTTGGCACAG AGGAGAGGAT TCCTATTCCC CTAATGGATT ATATTCTCAA TGTGATGAAA
    TTTGTGGAAT CAATTCTAAG TAACAACACA ACTGATGATC ACTGCCAGGA ATTTGTCAGT
    CAGAAGGGGC TCCTTCCACT TGTCACCATC CTTGGGCTTC CCAACCTACC TATTGACTTT
    CCTACCTCTG CTGCTTGCCA AGCTGTGGCA GGTGTCTGCA AATCCATTTT GACTCTTTCT
    CATGAGCCAA AAGTTCTCCA AGAAGGACTT CTTCAGCTGG ATTCAATTCT TTCATCTCTT
    GATCCACTTC ATCGGCCCAT TGAGGCTCCT GGTGGCTCAG TACTGCTTCG AGAGTTGGCA
    TGTGCTGGAA ATGTGGCAGA TGCTACTTTG TCTGCTCAAG CAACTCCATT ATTACACGCT
    CTCACTGCAG CCCATGCCTA CATCATGATG TTTGTGCATA CATGCAGGGT TGGACAGAGT
    GAAATCCGTG CCATCTCTGT GAACCAGTGG GGATCACAGC TGGGTCTCAA TGTTTTGAAC
    AAGTTGAGCC AGCTCTACTG CTCCCTTGTA TGGGAAAGTA CAGTTTTGCT GTCACTTTGT
    ACTCCTAACA GTTTGCCTCC TGGGTGTGAG TTTGGCCAAG CAGATATGCA GAAATTAGCC
    CCAAAGGAGG AAAAGGCAAC CACTGTATTG CCCCAAGGAA CCAAAAGACC TGAAAGTGAG
    GTTGAAGGCA CAGTTGTCAG TGAGGAAGCT TCAGGCTTAC TTGAAGGAAT TGGACTGGAT
    GGAGATACTT TAGCTCCAAT GGAAACTGAT GAACCCAGCA CTTCTGATGC AAAGGGCAAA
    CCGAAAATAA CCCCAGCTAT GGCGGCCAGA ATCAAGCAAA TCAAACCATT ACTATCAGCT
    TCTTCTAGGT TGGGCCGGGC TTTGGCAGAG CTGTTTGGGT TGCTTGTCAA ACTGTGTGTA
    GGTTCCCCTG TTCGGCAAAG GAGAAGTCAC CATGCTGCCA GCACTACCAC AGCCCCTACA
    CCAGCTGCTC GCTCCACAGC CTCTGCCCTC ACCAAACTGC TCACAAAAGG GCTGTCTTGG
    CAGCCTCCAC CCTATACCCC TACTCCTAGG TTCAGATTGA CTTTCTTTAT CTGCTCCGTT
    GGGTTCACAT CCCCCATGCT GTTTGATGAA AGGAAATACC CATATCACCT CATGCTGCAG
    AAATTCCTTT GCTCTGGTGG GCACAATGCA CTCTTTGAGA CTTTTAATTG GGCACTTTCA
    ATGGGTGGAA GAGTTCCTGT TGCTGAGGGC CTGGAACACA GTGATCTTCC TGATGGTACT
    GGAGAGTTTC TAGATGCTTG GCTTATGTTA GTGGAAAAGA TGGTGAATCC TACCACTGTG
    CTGGAATCTC CTCATTCACT TCCAACTACA AAGTTACCTG GAGGGCAGAA TAATCCACAG
    TTCAGTGCTT TACGCTTCCT TGTAGTCACA CAAAAGGCAG CTTTTACCTG TATCAAAAAC
    CTTTGGAATC GGAAACCCCT GAAAGTATAT GGAGGGCGTA TGGCAGAATC AATGCTTGCA
    ATCTTATGTC ATATACTTCG CGGTGAGCCA GTTATCCGTG AGCGTTTGAG CAAGGAGCGA
    GAGGGGAGCC GTGAAGAGGA GGGGCAGCAG GATGAGAGTG GAGCAAGACG GGAGCCTCAA
    GTGAACCAAC AGCAACTTCA GCAGGTGCTG ATGGATATGG GTTTCACAAG GGAGCATGCT
    CTGGAAGCTC TGCTTAACAC GAGTACTATG GAGCAGGCCA CTGAATATCT CCTCACCCAC
    CCTCCACCTC TTATTGGCGG AGTTGTACGG GAAACTGCTC GGCGGAAAGA AGAGGAGGAA
    CGCAAGGCAC GAGAAAAACA AGAGGAAGAG GAAGCTAAAT GCTTGGAAAA GTTTCAAGAT
    GCAGAACCCC TGGAACAGGA TGAGCTTCAA GGATTCACAG ACACCATGCT CCCTGGCTGC
    TTCCACTTGC TTGATGAGCT GCCAGACACA GTCTATAGAG TGTGTGATCT TATAATGACT
    GCCATAAAGC GCAATGGGGC AGACTATCGA GACATGATTC TAAAACAAGT GGTTAACCAG
    GTATGGGAAG CCGCTGATGT TCTTATTAAA GCTGCACTGC CGCTGACCAC AAGTGACACA
    AAAACTGTGT CTGAGTGGAT CAGCCAAATG GCTACTCTTC CTCAAGCTTC TAACTTGGCC
    ACTCGCATCC TTTTGCTCAC ACTGCTGTTT GAGGAGCTAA AACTGCCATG TGCTAGAGTT
    GTTGAAACAA GTGGAATACT TAACGTACTA ATTAAGCTGC TTGAAGTGGT TCAGCCATGT
    TTGCAGGCAG CCAAGGAGCA AAAGGAAGTT CAGACACCAA AGTGGATTAC ACCAGTCCTC
    CTCTTAATAG ATTTTTATGA GAAGACCTCA ATTTCTTCTA AAAGAAGAAT GCAAATGAAC
    AGGTACCTTC AAGCTAATGG AAACAACTGG CGATGGTTTG ATGATCGGTC TGGCCGTTGG
    TGCAGCTACA GTGCTAGTAA CAACAGCACT ATTGATGCAG CCTGGAAAGC AGGAGAAACA
    TGTGTGCGAT TTACAGCTGG CCGTCGAAGA TATACTGTGC AGTTTACCAC AATGGTTCAG
    GTAAATGAAG AGACCGGCAA TAGACGACCT GTCATGCTGA CTCTCTTGCG TGTTCCGAGA
    CTTTTAAAGA GTGGAAAAAA TGGAAATGGA CAGGAACTTG AAAAGACACT TGAGGAGGCA
    AAGGAGGTTG AAGCAAAGCA AAAAGACGAA AAAGTGCCTG AGCCCATTCC AGTTCCTGAA
    ACCTCTTCCA CTGAGAAAGA TGCACCAACT GAAGAGCCCA AAGTTGGAGA CATAGTAATT
    CAGGGTCTGG CTGAAGAAAT GGTGACCGTC CTTATACGTT CCTGCGTCGG CATGCTTGGT
    GTTCCTGTTG ATCCAGATAC TCTTCATGCA ACCTTGCGTC TTTGCCTGCG TCTCACACGT
    GACCACAAAT ATGCCATGAT GTTTGCAGAG CTTAAGAGCA CTCGAATGAT CCTCAGCTTA
    ACTCAAAGCT CTGGATTCAA TGGCTTTACT CCCCTTGTGA CGCTCCTATT TAGGCACATC
    ATAGAGGATC CTTGCACCCT CCAGCACACC ATGGAAAAGG TTGTACGGTC TGCAGCAACT
    AGTGGGGCAG GGAGTACCAC ATCTGGGGTT GTGTCTGGAA GCCTGGGTTC GCGTGAAATC
    AACTATATAC TTAGAGTACT GGGTCCAGCA GCTTGTCGAA ACCCTGACAT TTTCACTGAA
    GTGGCAAACA GTTGCATTCG CATTGCTCTC CCTGCACCTC GTGGATCTGG AACTGCCTCT
    GATGATGAGT TTGAGAACCT TCGGATCAAA GGTCCTAATG CAGTTCAGTT GGTTAAAACC
    ACCCCTATAA AGCCCTCCAC CTTGCCAGTC ATCCCTGATA CAATTAAGGA AGTAATCTAT
    GACATGCTGA ATGCACTGGC AGCTTACCAC GCCCCTGAGG AAGTATCTGA AAAGTCTGAG
    CAAAAACCTG TTGGCATGAA CCAGGAAGTC AGCCAGATCC TACAGGACAT GGGTGATGAG
    GTTTTCCAGC AATATCGGGA CTTAACCAGA CAGGGAAGCG ACTTTGATGG TCAGACCAGT
    TTTGCCATCA ACGCGCGAGT CTTTACTGCT GACAGCACAG CTGCCGAAAC ACCTCAGTCT
    GGTACACCTC AAGGTGAAAC TGCCTTAACA CCTGAAGAAA ACCGAGAAGG GTTAAAGAAA
    GATAAGGAAG GAGACCGTAG TTCAGAAGAT GGAAAGCAGA AGGCAAAAGG TAGTAAGCCT
    TTGCTGCCTA CCTCTTCCAT TCTTCGACTA CTTGCTGAAC TTGTCAGGTC CTATGTCGGA
    ATAGCCTCGC TGATCGCCAC CTACAATTAC ACTGCAGGAC AATCTGAACT AATTAAAGAG
    GATTGCAGTG TATTGGCGTT TGTGCTGGAT CACCTGCTAC CCCACACACA TAATGCAGAG
    GACAAGGACA CACCAGCCCT AGCCCGCCTC TTCTTAGCCA GCCTTGCTGC AGCAGGAAGT
    GGCACTGATG CCCAGGTTGC ACTAGTGAAT GAAGTAAAGG CAGCTCTTGG CAGAGCCCTA
    GCAATGGCTG AGAGCACGGA GAAGCATGCT AGGTTGCAGG CTGTGATGTG CATTATCAGT
    ACCATTATGG AGTCATGTCC ATCCACCTCC AGCTTCTACA GCACAGCAAC CACCAAAACC
    CAGCACAATG GCATGAACAA CATCATCAGA CTCTTTTTGA AAAAGGGGCT GGTTAGTGAT
    TTAGCCAGGG TCCCTCACAG CCTTGATTTA TCCAGTCCAA ATATGGCAAA CACCGTGAAT
    GCAGCACTGA AGCCACTCGA GACACTGTCT CGCATTGTCA ATCAACCCAG CAGTTTGTTT
    GGAAGCAAAG GGACTTCAGG AAAGAATAAA TCTGAGGCAG ATACACATGG AACAGCCCAG
    GATCCCAACA GTAATCAGCC AGATACAGGT GAAACAGGAA CAGAAGTGCA GGAAGAGGAC
    CCAGATGCTT CTCATGCTGA AGTAGCTGAT GGAGACATCA TGGATGGAGA GACAGAGACT
    GATGCTGTGG TTATTGCTGG CCAGCCTGAA GTCCTTACTA CACAAGAAAT GCAGGTAGAG
    AATGAGCTTG AAGATCTGAT AGATGAACTT CTTGAAAGGG ATGGTGCTGC AGGTAACCGG
    GTCGGAGAAG ATGAATCACA GGAAGATGTG CTGATGGATG AGCCTCCTAC TAACCTCAGC
    CAAGCCTCCA CTCTTCAGGC TAATCGAGAA GACTCTATGA ACATCTTAGA CCCAGAGGAT
    GAGGAAGAGC ACACCCAAGA AGAGGACAGC AGTGGCAGCA ATGAAGATGA AGATGACAGC
    CAGGAAGAGG AGGAGGAAGA GGAAGATGAG GACGATCAGG ATGATGAGGA GGGTGAGGAA
    GGGGATGAGG ATGATGATGA TGACTGTTCA GAGATGGAAC TGGATGAGGA TTATCCAGAC
    ATGAATGCCT CACCTCTTGT ACGCTTTGAA CGTTTTGACA GAGAAGATGA TTTAATCATA
    GAGTTTGATA ACATGTTCTC CAGTGCCACT GAGATCCCTC CATCCCCTGG TAACATCCCT
    ACTACACACC CCCTAATGGT GCGACACGCT GATCACGGCT CCTTGACTCT TGGCAGTGGC
    ACTTCCACTA CTCGCCTGGC ACAGGGCATC GGGCGCAGCC AGAGAACACT CCGACAACTC
    ACAGCCAACA CCGGTCACAC AATACATGTT CATTATCCTG GCAACAGGCA GCCAAATCCA
    CCTTTAATCC TCCAGAGGTT GCTGGGGCCC TCAGCCGCTG CTGACATCTT GCAACTAAGC
    AGCAGTCTTC CTCTGCAGAG TCGTGGCCGA GCACGTCTAC TTGTTGGCAA CGATGATGTC
    CACATCATAG CACGCTCTGA TGATGAGATG CTTGATGATT TTTTCCATGA TCAGAGTACA
    GCCACAAGTC AGGCTGGCAC ACTTTCCAGC ATTCCAACTG CGCTGACCCG CTGGACAGAA
    GAGTGTAAGG TCTTGGATGC TGAAAGCATG CATGACTGTG TATCTGTGGT CAAAGTCCCA
    ATCATCAATC ATTTGGAATC TTTGCGTGAT GAGGAGCTTG AGGAGCGCAG GGAAAAAAGA
    AGGAAACAAC TAGCAGAAGA GGAGGCCAAG ATGGCAGAGA AATCCAAGGA GGAGAAGGAA
    GCAAAGGAGC AAAATTCACA GGGTGCCAAT TCAAAGCAGA ATACCCCATT TGAACTGAGC
    CTTGCAGATC AAGATGGAAG TGGTCGTCCT GAAAATTTCC CAACTGCACC ATCTTCTGCA
    GAAGGAACTC CAACAAGTCC TGAAATTGTT CGTTTAGATC CCACCCTAAG GGCTACTGAG
    GCTACTCTGG CAGAGCTGAC TCAGGGTCTG CCTGCTGATA CAGGCACGGA GGATGTTGGG
    CAACAACTCA TGCCATCTCA CCAAGAGGAC TCTGGACCAG GACGGCCAAG TGTAGAGGGG
    GATGCCACAC AAATGGAGCT ATCGCCAGCA CCCACTATAA CCTCCTTATC ACCAGAGCGA
    GCAGAGGATT CAGATGCTTT GACAGCTGTT AGTAGCCAAC TAGAGGGCTC GCCTATGGAC
    ACCAGCAGTC TTGCCTCTGG GCCCTTAGAG GATGGCGTAG GGGAGGCACC TGGAATTGTT
    AATCCAGAGC AGCCTCTTGT GGTTGGCAGT AGCATTGCTG TTGGACAGCA ACCAGTGGAA
    CCACCGACGA CCTGTGAAAC CCAACCAGTA GTTTCTCAAC ACAGTGATAG CTCACAAATT
    GCCCCTTCCT CTGAGTTGCC TTCCACAAGA GAAAGTGCTG TGACCTTGTT GGCTACAGAT
    TCTAGTGGTA TCCTTGAAGA ACCTTTGCCT TCTACAAGCA GTGAAGAGGA AGACCCATTA
    GCAGGCATAA ATCTTCCAGA GGGTGTGGAT CCTTCTTTCC TAGCGGCTCT TCCTGAAGAC
    ATCCGCAGAG AGGTGCTGCA GAATCAGTTG GGCATACGGC CGCCATCGCG ACCTCCACCC
    ACTGCTGCCC CAGTTATTCC TCCTCCTGTG GTTGCTAATG CTGGTGTCAC GGAGGTCAGC
    CCTGAGTTCT TGGCAGCGTT GCCACCAGCC ATTCAGGAAG AGGTCCTAGC ACAGCAGCGA
    GCAGAACAGC AGAGGCGGGA ACTTGCTCAG ACCACTACGT CTGATACACC CATGGATCCT
    GTGACTTTCA TTCAGACGTT ACCTTCTGAT CTACGTCGTA GTGTGTTAGA AGACATGGAG
    GACAGTGTTT TAGCTGTGAT GCCACCTGAC ATTGCTGCAG AGGCACAGGC TCTAAGGCGA
    GAGCAAGAAG CTCGGCAGAG GCAACTAATG CATGAACGAC TGTTCGGGCA CAGTAGCACA
    TCTGCACTCT CTGCCATCCT TAGGAGTCCT GCTTTCACGA GTCGCTTGAG TGGAAACCGA
    GGTGTTCAGT ACACACGGTT GGCTGTACAG AGAGGGAACA CTTTCCAAAT GGGAAGCAGC
    AGTACACATA CCAGACCAAC CGGTAGTAAT GTTGACAGCC TACTACGATT ACGAGGCCGA
    CTATTACTTG ATCATGAAGC CCTCTCCTGT CTGCTTGTCC TACTGTTTGT TGATGAACCC
    AAGCTAAACA CCAGTCGCTT GCACCGTGTT CTACGCAACC TCTGCTACCA TGCACAAACA
    AGGAACTGGG TGATTAGAAG CTTGTTGTCT ATACTGCAGC GTAGCAGTGA AAGTGAGCTT
    TGTATAGAAA GCCCAAAGAC TTCTATTGGG GGCACTGATA AACGGGGTGC AAAAACAAGT
    CATGACAACC GCCCTTTAGA ATTTCTGCAT AAGATGGAAT CGAAGAATTC CAACCAGCTC
    TCCTGGCTTT CTGTATCCAT GGACGCTGCT CTTGGCTGCC GTACGAACAT CTTCCAAATC
    CAGAGGTCCC TAGGACGCAA GCATACAGAA AAGCATTCAG CCTGTGGTTC TACTGTCCAC
    ATCCATCCCC AAGCAGCCCC TGTAGTGTGT AGACATGTGC TTGACACACT AATACAGCTA
    GCTAAGGTGT TTCCCAGTCA TTTCACACAG CAGCGAACCA AAGAATGTAG TGTGGAAAAC
    AGTGACAGCA GGGAGCGGAG TATAAAACTT ACTTGTAGTC CGTGTCTTGC ACCATCTAGT
    AGTATTGGAG CAAGCAACAT CTCCACAGAT TTCTGGGACT TGCTAGTCAA GCTAGACAAC
    ATGAATGTGA GCCGGAAGGG GAAAACGTCT ATAAAATCTG TGCCAGTGAG CTCTGGTTCT
    GAAGGCGAGA GCAATCAGTG CAGCCTGGAG GCCTCCCCCT TAGGACAGCT TATGAATATG
    CTGTCTCATT CTGTTATTCG CCGCAGTTCG CTGCTTACTG AAAAGCTGCT GAGACTTCTC
    TCCCTTATCT CTATTGCCCT TCCTGATAAT AAGGCTTCTG AAACAGCAGG CAATCAGAGC
    AATTCTATTA CCCCAGCACC TGCACCTACG CCTGCAACTG CACCAGCCCC TGTGCCTGTA
    CCTGCTGTAA CAGTTCCAGT TGTTGTGAGC ACAAACATAG TGACAACTGT TACAACCAAA
    AGTGGAAAAT CTCCTGCAAA ATCAAGTGAA GTCAGCAATG CACCAGATAA GATGGCAGCT
    TCTGGCCTTA CAGAGAAACA GCTGCAGTTG TCTGTTGAGG TTCTGACATC ACACTCGTGT
    TCAGAAGAAG GACTGGAGGA TGCCGCCAAT GTTCTACTTC AGCTTTCCAG AGGCGACCCC
    AGTACACGGG ACACTGTACT GAAACTTCTG CTTAGCGGAG CACATCACTT GGGGTTTACT
    CTCTGCAAAC AGATAGGTAC ACTACTAGCA GAGCTCCGGG AATATAACCT AGAGCAGCAG
    CGTCGTGCAC AGTGTGAAGT TCTTTCTCCA GATGGTATGC CTGAGGAGCA ACCCCACAGC
    ACAAAGCCAA AAGGCAAAAT GCAGAGCAGT GTTAGGTCTC TCCCCACCCC ACCTATGCGA
    TCCAGGTTCG ACATTGCAGA GAATGTGGTG ATTGTGGCTT CCCAAAAGCG TCCACTGGGA
    GGAAGAGAGC TGCAGCTCCC CTCAATGTCT ATGCTGACGT CAAAGACTTC CACACAAAAA
    TTCTTTCTTC GAGTGCTGCA GGTTATCATC CAGCTTCGCG ATGACACTCG CAGAGCCAAC
    AAGAAGGCCA AGCAGACCAG CAGATTAGGC ACTGCCAGTC TGGCCTCGGC TAGCAGCATC
    CAAGCAGCTG TGCAGCAGCT GGAGGCTGAG GCTGATGCCA TTATACAAAT GGTACGTGAG
    GGTCAAAGGG CGCGGAGACA GCTGCAAACT GCAACGGCGG AGGGTTCTGC TCGCAGGGAG
    GAGCAGCCTA TGGATGTTGA CCAGCCTTCG CCTGCTACAC AGGAGCCTCA GTCAGTTGCT
    TCTGAAGGTT CTCAGGCTGC TGAAAAAAAA GAAGAGGAGA GGTTACCAGA GTTACCGCTG
    TTAAGTGAGC AGCTAAGTTT AGATGAGCTG TGGGACATGT TGGGAGAGTG CCTGAAGGAA
    CTTGAGGAAT CTCATGACCA GCATGCCGTA TTAGTGTTAC AGCCAGCCGT AGAAGCCTTT
    TTCCTTGTTC ATGCAACAGA GCGGGAGAGC AAGCCTCCTG TGCGGGACAC TCGGGAAAGC
    CAGTTATCTC ACATTAAGGA TGAACCACCT CCCCTGTCTC CTGCACCCCT CACTCCAGCC
    ACACCTTCTT CCTTGGATCC TTTCTTCTCT CGTGAACCTT CATCTATGCA CATTTCCTCT
    AGTTTGCCTC CTGACACTCA GAAGTTCCTA AGATTTGCTG AGACTCATCG GACTGTATTA
    AATCAGATCC TTCGCCAGTC CACCACACAC CTAGCTGATG GGCCCTTTGC TGTGCTTGTA
    GACTATATCA GAGTCCTTGA CTTTGATGTT AAGCGCAAAT ATTTCCGACA GGAGTTGGAG
    CGACTGGATG AAGGGCTAAG AAAAGAGGAT ATGGCAGTGC ATGTGCGGCG AGATCATGTG
    TTTGAGGATT CCTACCGGGA GCTGCACCGC AAATCCCCTG AAGAAATGAA GAATAGATTG
    TACATAGTAT TTGAAGGAGA AGAAGGGCAG GATGCAGGTG GGCTGCTTCG GGAGTGGTAC
    ATGATTATCT CCAGGGAGAT GTTCAATCCC ATGTATGCTT TGTTCCGCAC TTCCCCAGGT
    GACCGCGTCA CCTACACTAT TAATCCTTCC TCCCACTGTA ACCCTAACCA CCTCAGCTAC
    TTCAAGTTTG TGGGCCGGAT AGTTGCCAAA GCGGTTTATG ACAACCGGCT ACTGGAGTGT
    TACTTCACTA GGTCCTTCTA CAAGCATATA TTGGGCAAGT CTGTCAGATA CACAGACATG
    GAGAGTGAAG ATTATCACTT CTATCAGGGT CTGGTGTACT TGCTTGAAAA TGATGTCTCC
    ACACTGGGAT ATGACTTGAC TTTCAGCACT GAGGTTCAAG AATTTGGTGT CTGTGAAGTC
    CGAGACCTAA AACCAAACGG TGCAAACATT CTGGTAACAG AAGAAAACAA AAAGGAGTAT
    GTTCATCTTG TGTGCCAGAT GAAGATGACA GGTGCAATAC GTAAGCAATT GGCTGCCTTC
    CTTGAGGGAT TTTATGAGAT AATCCCCAAA CGTCTGATCT CCATCTTCAC TGAGCAGGAA
    TTGGAACTGC TTATCTCTGG CCTTCCCACC ATAGATATTG ATGACCTAAA ATCAAACACT
    GAGTATCACA AATACCAGTC TAACTCCATC CAGATTCAGT GGTTCTGGAG AGCGTTGCGC
    TCATTCGATC AAGCTGACCG GGCAAAGTTC CTTCAGTTTG TTACTGGAAC TTCAAAGGTC
    CCATTACAGG GTTTTGCTGC GCTGGAAGGA ATGAACGGCA TCCAGAAATT CCAAATCCAC
    AGAGATGATA GATCTACAGA CAGGCTGCCT TCTGCGCACA CGTGCTTCAA CCAGTTGGAC
    CTTCCAGCCT ATGAGAGTTA CGAGAAGCTA CGTCACATGC TGCTGTTAGC CATCCAGGAG
    TGCTCTGAGG GCTTTGGCCT TGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65597
    Clone ID Related Accession (Same CDS sequence) XM_031892177.1
    Accession Version XM_031892177.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
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    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
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    1801
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    1981
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    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGGCGAT ACAGCCTCTA ACCTGGGCAG TGCTGTTGAT
    GAACTGATGA GGCACCAGCC CACGTTAAAA ACAGACGCTA CAACTGCCAT CATTAAGCTC
    TTAGAAGAGA TATGTAACCT TGGTAGGGAC CCAAAATACA TCTGTCAGAA GCCATCTATT
    CAGAAAGCTG ATGGCACGGC TACTGCTCCT CCACCACGTT CCAACCATGC AGCAGAGGAG
    GCTTCTAGTG AGGATGAAGA GGAGGAGGAG GTACAAGCAA TGCAGAGTTT CAGCACAAAT
    CAGCAAAGTG AAGTTGAACC CAGCCAGCAT GTTGTTGGCA CAGAGGAGAG GATTCCTATT
    CCCCTAATGG ATTATATTCT CAATGTGATG AAATTTGTGG AATCAATTCT AAGTAACAAC
    ACAACTGATG ATCACTGCCA GGAATTTGTC AGTCAGAAGG GGCTCCTTCC ACTTGTCACC
    ATCCTTGGGC TTCCCAACCT ACCTATTGAC TTTCCTACCT CTGCTGCTTG CCAAGCTGTG
    GCAGGTGTCT GCAAATCCAT TTTGACTCTT TCTCATGAGC CAAAAGTTCT CCAAGAAGGA
    CTTCTTCAGC TGGATTCAAT TCTTTCATCT CTTGATCCAC TTCATCGGCC CATTGAGGCT
    CCTGGTGGCT CAGTACTGCT TCGAGAGTTG GCATGTGCTG GAAATGTGGC AGATGCTACT
    TTGTCTGCTC AAGCAACTCC ATTATTACAC GCTCTCACTG CAGCCCATGC CTACATCATG
    ATGTTTGTGC ATACATGCAG GGTTGGACAG AGTGAAATCC GTGCCATCTC TGTGAACCAG
    TGGGGATCAC AGCTGGGTCT CAATGTTTTG AACAAGTTGA GCCAGCTCTA CTGCTCCCTT
    GTATGGGAAA GTACAGTTTT GCTGTCACTT TGTACTCCTA ACAGTTTGCC TCCTGGGTGT
    GAGTTTGGCC AAGCAGATAT GCAGAAATTA GCCCCAAAGG AGGAAAAGGC AACCACTGTA
    TTGCCCCAAG GAACCAAAAG ACCTGAAAGT GAGGTTGAAG GCACAGTTGT CAGTGAGGAA
    GCTTCAGGCT TACTTGAAGG AATTGGACTG GATGGAGATA CTTTAGCTCC AATGGAAACT
    GATGAACCCA GCACTTCTGA TGCAAAGGGC AAACCGAAAA TAACCCCAGC TATGGCGGCC
    AGAATCAAGC AAATCAAACC ATTACTATCA GCTTCTTCTA GGTTGGGCCG GGCTTTGGCA
    GAGCTGTTTG GGTTGCTTGT CAAACTGTGT GTAGGTTCCC CTGTTCGGCA AAGGAGAAGT
    CACCATGCTG CCAGCACTAC CACAGCCCCT ACACCAGCTG CTCGCTCCAC AGCCTCTGCC
    CTCACCAAAC TGCTCACAAA AGGGCTGTCT TGGCAGCCTC CACCCTATAC CCCTACTCCT
    AGGTTCAGAT TGACTTTCTT TATCTGCTCC GTTGGGTTCA CATCCCCCAT GCTGTTTGAT
    GAAAGGAAAT ACCCATATCA CCTCATGCTG CAGAAATTCC TTTGCTCTGG TGGGCACAAT
    GCACTCTTTG AGACTTTTAA TTGGGCACTT TCAATGGGTG GAAGAGTTCC TGTTGCTGAG
    GGCCTGGAAC ACAGTGATCT TCCTGATGGT ACTGGAGAGT TTCTAGATGC TTGGCTTATG
    TTAGTGGAAA AGATGGTGAA TCCTACCACT GTGCTGGAAT CTCCTCATTC ACTTCCAACT
    ACAAAGTTAC CTGGAGGGCA GAATAATCCA CAGTTCAGTG CTTTACGCTT CCTTGTAGTC
    ACACAAAAGG CAGCTTTTAC CTGTATCAAA AACCTTTGGA ATCGGAAACC CCTGAAAGTA
    TATGGAGGGC GTATGGCAGA ATCAATGCTT GCAATCTTAT GTCATATACT TCGCGGTGAG
    CCAGTTATCC GTGAGCGTTT GAGCAAGGAG CGAGAGGGGA GCCGTGAAGA GGAGGGGCAG
    CAGGATGAGA GTGGAGCAAG ACGGGAGCCT CAAGTGAACC AACAGCAACT TCAGCAGCTG
    ATGGATATGG GTTTCACAAG GGAGCATGCT CTGGAAGCTC TGCTTAACAC GAGTACTATG
    GAGCAGGCCA CTGAATATCT CCTCACCCAC CCTCCACCTC TTATTGGCGG AGTTGTACGG
    GACTTAAGTA TGTCAGAGGA AGATCAGATG CTGAGAGCCA TTGCCATGTC GCTGGGACAA
    GATATCCCTA TGGAACAGAG GGCAGAACTG CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG
    GAGGAACGCA AGGCACGAGA AAAACAAGAG GAAGAGGAAG CTAAATGCTT GGAAAAGTTT
    CAAGATGCAG AACCCCTGGA ACAGGATGAG CTTCAAGGAT TCACAGACAC CATGCTCCCT
    GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA GACACAGTCT ATAGAGTGTG TGATCTTATA
    ATGACTGCCA TAAAGCGCAA TGGGGCAGAC TATCGAGACA TGATTCTAAA ACAAGTGGTT
    AACCAGGTAT GGGAAGCCGC TGATGTTCTT ATTAAAGCTG CACTGCCGCT GACCACAAGT
    GACACAAAAA CTGTGTCTGA GTGGATCAGC CAAATGGCTA CTCTTCCTCA AGCTTCTAAC
    TTGGCCACTC GCATCCTTTT GCTCACACTG CTGTTTGAGG AGCTAAAACT GCCATGTGCT
    AGAGTTGTTG AAACAAGTGG AATACTTAAC GTACTAATTA AGCTGCTTGA AGTGGTTCAG
    CCATGTTTGC AGGCAGCCAA GGAGCAAAAG GAAGTTCAGA CACCAAAGTG GATTACACCA
    GTCCTCCTCT TAATAGATTT TTATGAGAAG ACCTCAATTT CTTCTAAAAG AAGAATGCAA
    ATGAACAGGT ACCTTCAAGC TAATGGAAAC AACTGGCGAT GGTTTGATGA TCGGTCTGGC
    CGTTGGTGCA GCTACAGTGC TAGTAACAAC AGCACTATTG ATGCAGCCTG GAAAGCAGGA
    GAAACATGTG TGCGATTTAC AGCTGGCCGT CGAAGATATA CTGTGCAGTT TACCACAATG
    GTTCAGGTAA ATGAAGAGAC CGGCAATAGA CGACCTGTCA TGCTGACTCT CTTGCGTGTT
    CCGAGACTTT TAAAGAGTGG AAAAAATGGA AATGGACAGG AACTTGAAAA GACACTTGAG
    GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA GACGAAAAAG TGCCTGAGCC CATTCCAGTT
    CCTGAAACCT CTTCCACTGA GAAAGATGCA CCAACTGAAG AGCCCAAAGT TGGAGACATA
    GTAATTCAGG GTCTGGCTGA AGAAATGGTG ACCGTCCTTA TACGTTCCTG CGTCGGCATG
    CTTGGTGTTC CTGTTGATCC AGATACTCTT CATGCAACCT TGCGTCTTTG CCTGCGTCTC
    ACACGTGACC ACAAATATGC CATGATGTTT GCAGAGCTTA AGAGCACTCG AATGATCCTC
    AGCTTAACTC AAAGCTCTGG ATTCAATGGC TTTACTCCCC TTGTGACGCT CCTATTTAGG
    CACATCATAG AGGATCCTTG CACCCTCCAG CACACCATGG AAAAGGTTGT ACGGTCTGCA
    GCAACTAGTG GGGCAGGGAG TACCACATCT GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT
    GAAATCAACT ATATACTTAG AGTACTGGGT CCAGCAGCTT GTCGAAACCC TGACATTTTC
    ACTGAAGTGG CAAACAGTTG CATTCGCATT GCTCTCCCTG CACCTCGTGG ATCTGGAACT
    GCCTCTGATG ATGAGTTTGA GAACCTTCGG ATCAAAGGTC CTAATGCAGT TCAGTTGGTT
    AAAACCACCC CTATAAAGCC CTCCACCTTG CCAGTCATCC CTGATACAAT TAAGGAAGTA
    ATCTATGACA TGCTGAATGC ACTGGCAGCT TACCACGCCC CTGAGGAAGT ATCTGAAAAG
    TCTGAGCAAA AACCTGTTGG CATGAACCAG GAAGTCAGCC AGATCCTACA GGACATGGGT
    GATGAGGTTT TCCAGCAATA TCGGGACTTA ACCAGACAGG GAAGCGACTT TGATGGTCAG
    ACCAGTTTTG CCATCAACGC GCGAGTCTTT ACTGCTGACA GCACAGCTGC CGAAACACCT
    CAGTCTGGTA CACCTCAAGG TGAAACTGCC TTAACACCTG AAGAAAACCG AGAAGGGTTA
    AAGAAAGATA AGGAAGGAGA CCGTAGTTCA GAAGATGGAA AGCAGAAGGC AAAAGGTAGT
    AAGCCTTTGC TGCCTACCTC TTCCATTCTT CGACTACTTG CTGAACTTGT CAGGTCCTAT
    GTCGGAATAG CCTCGCTGAT CGCCACCTAC AATTACACTG CAGGACAATC TGAACTAATT
    AAAGAGGATT GCAGTGTATT GGCGTTTGTG CTGGATCACC TGCTACCCCA CACACATAAT
    GCAGAGGACA AGGACACACC AGCCCTAGCC CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA
    GGAAGTGGCA CTGATGCCCA GGTTGCACTA GTGAATGAAG TAAAGGCAGC TCTTGGCAGA
    GCCCTAGCAA TGGCTGAGAG CACGGAGAAG CATGCTAGGT TGCAGGCTGT GATGTGCATT
    ATCAGTACCA TTATGGAGTC ATGTCCATCC ACCTCCAGCT TCTACAGCAC AGCAACCACC
    AAAACCCAGC ACAATGGCAT GAACAACATC ATCAGACTCT TTTTGAAAAA GGGGCTGGTT
    AGTGATTTAG CCAGGGTCCC TCACAGCCTT GATTTATCCA GTCCAAATAT GGCAAACACC
    GTGAATGCAG CACTGAAGCC ACTCGAGACA CTGTCTCGCA TTGTCAATCA ACCCAGCAGT
    TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG AATAAATCTG AGGCAGATAC ACATGGAACA
    GCCCAGGATC CCAACAGTAA TCAGCCAGAT ACAGGTGAAA CAGGAACAGA AGTGCAGGAA
    GAGGACCCAG ATGCTTCTCA TGCTGAAGTA GCTGATGGAG ACATCATGGA TGGAGAGACA
    GAGACTGATG CTGTGGTTAT TGCTGGCCAG CCTGAAGTCC TTACTACACA AGAAATGCAG
    GTAGAGAATG AGCTTGAAGA TCTGATAGAT GAACTTCTTG AAAGGGATGG TGCTGCAGGT
    AACCGGGTCG GAGAAGATGA ATCACAGGAA GATGTGCTGA TGGATGAGCC TCCTACTAAC
    CTCAGCCAAG CCTCCACTCT TCAGGCTAAT CGAGAAGACT CTATGAACAT CTTAGACCCA
    GAGGATGAGG AAGAGCACAC CCAAGAAGAG GACAGCAGTG GCAGCAATGA AGATGAAGAT
    GACAGCCAGG AAGAGGAGGA GGAAGAGGAA GATGAGGACG ATCAGGATGA TGAGGAGGGT
    GAGGAAGGGG ATGAGGATGA TGATGATGAC TGTTCAGAGA TGGAACTGGA TGAGGATTAT
    CCAGACATGA ATGCCTCACC TCTTGTACGC TTTGAACGTT TTGACAGAGA AGATGATTTA
    ATCATAGAGT TTGATAACAT GTTCTCCAGT GCCACTGAGA TCCCTCCATC CCCTGGTAAC
    ATCCCTACTA CACACCCCCT AATGGTGCGA CACGCTGATC ACGGCTCCTT GACTCTTGGC
    AGTGGCACTT CCACTACTCG CCTGGCACAG GGCATCGGGC GCAGCCAGAG AACACTCCGA
    CAACTCACAG CCAACACCGG TCACACAATA CATGTTCATT ATCCTGGCAA CAGGCAGCCA
    AATCCACCTT TAATCCTCCA GAGGTTGCTG GGGCCCTCAG CCGCTGCTGA CATCTTGCAA
    CTAAGCAGCA GTCTTCCTCT GCAGAGTCGT GGCCGAGCAC GTCTACTTGT TGGCAACGAT
    GATGTCCACA TCATAGCACG CTCTGATGAT GAGATGCTTG ATGATTTTTT CCATGATCAG
    AGTACAGCCA CAAGTCAGGC TGGCACACTT TCCAGCATTC CAACTGCGCT GACCCGCTGG
    ACAGAAGAGT GTAAGGTCTT GGATGCTGAA AGCATGCATG ACTGTGTATC TGTGGTCAAA
    GTCCCAATCA TCAATCATTT GGAATCTTTG CGTGATGAGG AGCTTGAGGA GCGCAGGGAA
    AAAAGAAGGA AACAACTAGC AGAAGAGGAG GCCAAGATGG CAGAGAAATC CAAGGAGGAG
    AAGGAAGCAA AGGAGCAAAA TTCACAGGGT GCCAATTCAA AGCAGAATAC CCCATTTGAA
    CTGAGCCTTG CAGATCAAGA TGGAAGTGGT CGTCCTGAAA ATTTCCCAAC TGCACCATCT
    TCTGCAGAAG GAACTCCAAC AAGTCCTGAA ATTGTTCGTT TAGATCCCAC CCTAAGGGCT
    ACTGAGGCTA CTCTGGCAGA GCTGACTCAG GGTCTGCCTG CTGATACAGG CACGGAGGAT
    GTTGGGCAAC AACTCATGCC ATCTCACCAA GAGGACTCTG GACCAGGACG GCCAAGTGTA
    GAGGGGGATG CCACACAAAT GGAGCTATCG CCAGCACCCA CTATAACCTC CTTATCACCA
    GAGCGAGCAG AGGATTCAGA TGCTTTGACA GCTGTTAGTA GCCAACTAGA GGGCTCGCCT
    ATGGACACCA GCAGTCTTGC CTCTGGGCCC TTAGAGGATG GCGTAGGGGA GGCACCTGGA
    ATTGTTAATC CAGAGCAGCC TCTTGTGGTT GGCAGTAGCA TTGCTGTTGG ACAGCAACCA
    GTGGAACCAC CGACGACCTG TGAAACCCAA CCAGTAGTTT CTCAACACAG TGATAGCTCA
    CAAATTGCCC CTTCCTCTGA GTTGCCTTCC ACAAGAGAAA GTGCTGTGAC CTTGTTGGCT
    ACAGATTCTA GTGGTATCCT TGAAGAACCT TTGCCTTCTA CAAGCAGTGA AGAGGAAGAC
    CCATTAGCAG GCATAAATCT TCCAGAGGGT GTGGATCCTT CTTTCCTAGC GGCTCTTCCT
    GAAGACATCC GCAGAGAGGT GCTGCAGAAT CAGTTGGGCA TACGGCCGCC ATCGCGACCT
    CCACCCACTG CTGCCCCAGT TATTCCTCCT CCTGTGGTTG CTAATGCTGG TGTCACGGAG
    GTCAGCCCTG AGTTCTTGGC AGCGTTGCCA CCAGCCATTC AGGAAGAGGT CCTAGCACAG
    CAGCGAGCAG AACAGCAGAG GCGGGAACTT GCTCAGACCA CTACGTCTGA TACACCCATG
    GATCCTGTGA CTTTCATTCA GACGTTACCT TCTGATCTAC GTCGTAGTGT GTTAGAAGAC
    ATGGAGGACA GTGTTTTAGC TGTGATGCCA CCTGACATTG CTGCAGAGGC ACAGGCTCTA
    AGGCGAGAGC AAGAAGCTCG GCAGAGGCAA CTAATGCATG AACGACTGTT CGGGCACAGT
    AGCACATCTG CACTCTCTGC CATCCTTAGG AGTCCTGCTT TCACGAGTCG CTTGAGTGGA
    AACCGAGGTG TTCAGTACAC ACGGTTGGCT GTACAGAGAG GGAACACTTT CCAAATGGGA
    AGCAGCAGTA CACATACCAG ACCAACCGGT AGTAATGTTG ACAGCCTACT ACGATTACGA
    GGCCGACTAT TACTTGATCA TGAAGCCCTC TCCTGTCTGC TTGTCCTACT GTTTGTTGAT
    GAACCCAAGC TAAACACCAG TCGCTTGCAC CGTGTTCTAC GCAACCTCTG CTACCATGCA
    CAAACAAGGA ACTGGGTGAT TAGAAGCTTG TTGTCTATAC TGCAGCGTAG CAGTGAAAGT
    GAGCTTTGTA TAGAAAGCCC AAAGACTTCT ATTGGGGGCA CTGATAAACG GGGTGCAAAA
    ACAAGTCATG ACAACCGCCC TTTAGAATTT CTGCATAAGA TGGAATCGAA GAATTCCAAC
    CAGCTCTCCT GGCTTTCTGT ATCCATGGAC GCTGCTCTTG GCTGCCGTAC GAACATCTTC
    CAAATCCAGA GGTCCCTAGG ACGCAAGCAT ACAGAAAAGC ATTCAGCCTG TGGTTCTACT
    GTCCACATCC ATCCCCAAGC AGCCCCTGTA GTGTGTAGAC ATGTGCTTGA CACACTAATA
    CAGCTAGCTA AGGTGTTTCC CAGTCATTTC ACACAGCAGC GAACCAAAGA ATGTAGTGTG
    GAAAACAGTG ACAGCAGGGA GCGGAGTATA AAACTTACTT GTAGTCCGTG TCTTGCACCA
    TCTAGTAGTA TTGGAGCAAG CAACATCTCC ACAGATTTCT GGGACTTGCT AGTCAAGCTA
    GACAACATGA ATGTGAGCCG GAAGGGGAAA ACGTCTATAA AATCTGTGCC AGTGAGCTCT
    GGTTCTGAAG GCGAGAGCAA TCAGTGCAGC CTGGAGGCCT CCCCCTTAGG ACAGCTTATG
    AATATGCTGT CTCATTCTGT TATTCGCCGC AGTTCGCTGC TTACTGAAAA GCTGCTGAGA
    CTTCTCTCCC TTATCTCTAT TGCCCTTCCT GATAATAAGG CTTCTGAAAC AGCAGGCAAT
    CAGAGCAATT CTATTACCCC AGCACCTGCA CCTACGCCTG CAACTGCACC AGCCCCTGTG
    CCTGTACCTG CTGTAACAGT TCCAGTTGTT GTGAGCACAA ACATAGTGAC AACTGTTACA
    ACCAAAAGTG GAAAATCTCC TGCAAAATCA AGTGAAGTCA GCAATGCACC AGATAAGATG
    GCAGCTTCTG GCCTTACAGA GAAACAGCTG CAGTTGTCTG TTGAGGTTCT GACATCACAC
    TCGTGTTCAG AAGAAGGACT GGAGGATGCC GCCAATGTTC TACTTCAGCT TTCCAGAGGC
    GACCCCAGTA CACGGGACAC TGTACTGAAA CTTCTGCTTA GCGGAGCACA TCACTTGGGG
    TTTACTCTCT GCAAACAGAT AGGTACACTA CTAGCAGAGC TCCGGGAATA TAACCTAGAG
    CAGCAGCGTC GTGCACAGTG TGAAGTTCTT TCTCCAGATG GTATGCCTGA GGAGCAACCC
    CACAGCACAA AGCCAAAAGG CAAAATGCAG AGCAGGTTCG ACATTGCAGA GAATGTGGTG
    ATTGTGGCTT CCCAAAAGCG TCCACTGGGA GGAAGAGAGC TGCAGCTCCC CTCAATGTCT
    ATGCTGACGT CAAAGACTTC CACACAAAAA TTCTTTCTTC GAGTGCTGCA GGTTATCATC
    CAGCTTCGCG ATGACACTCG CAGAGCCAAC AAGAAGGCCA AGCAGACCAG CAGATTAGGC
    ACTGCCAGTC TGGCCTCGGC TAGCAGCATC CAAGCAGCTG TGCAGCAGCT GGAGGCTGAG
    GCTGATGCCA TTATACAAAT GGTACGTGAG GGTCAAAGGG CGCGGAGACA GCTGCAAACT
    GCAACGGCGG AGGGTTCTGC TCGCAGGGAG GAGCAGCCTA TGGATGTTGA CCAGCCTTCG
    CCTGCTACAC AGGAGCCTCA GTCAGTTGCT TCTGAAGGTT CTCAGGCTGC TGAAAAAAAA
    GAAGAGGAGA GGTTACCAGA GTTACCGCTG TTAAGTGAGC AGCTAAGTTT AGATGAGCTG
    TGGGACATGT TGGGAGAGTG CCTGAAGGAA CTTGAGGAAT CTCATGACCA GCATGCCGTA
    TTAGTGTTAC AGCCAGCCGT AGAAGCCTTT TTCCTTGTTC ATGCAACAGA GCGGGAGAGC
    AAGCCTCCTG TGCGGGACAC TCGGGAAAGC CAGTTATCTC ACATTAAGGA TGAACCACCT
    CCCCTGTCTC CTGCACCCCT CACTCCAGCC ACACCTTCTT CCTTGGATCC TTTCTTCTCT
    CGTGAACCTT CATCTATGCA CATTTCCTCT AGTTTGCCTC CTGACACTCA GAAGTTCCTA
    AGATTTGCTG AGACTCATCG GACTGTATTA AATCAGATCC TTCGCCAGTC CACCACACAC
    CTAGCTGATG GGCCCTTTGC TGTGCTTGTA GACTATATCA GAGTCCTTGA CTTTGATGTT
    AAGCGCAAAT ATTTCCGACA GGAGTTGGAG CGACTGGATG AAGGGCTAAG AAAAGAGGAT
    ATGGCAGTGC ATGTGCGGCG AGATCATGTG TTTGAGGATT CCTACCGGGA GCTGCACCGC
    AAATCCCCTG AAGAAATGAA GAATAGATTG TACATAGTAT TTGAAGGAGA AGAAGGGCAG
    GATGCAGGTG GGCTGCTTCG GGAGTGGTAC ATGATTATCT CCAGGGAGAT GTTCAATCCC
    ATGTATGCTT TGTTCCGCAC TTCCCCAGGT GACCGCGTCA CCTACACTAT TAATCCTTCC
    TCCCACTGTA ACCCTAACCA CCTCAGCTAC TTCAAGTTTG TGGGCCGGAT AGTTGCCAAA
    GCGGTTTATG ACAACCGGCT ACTGGAGTGT TACTTCACTA GGTCCTTCTA CAAGCATATA
    TTGGGCAAGT CTGTCAGATA CACAGACATG GAGAGTGAAG ATTATCACTT CTATCAGGGT
    CTGGTGTACT TGCTTGAAAA TGATGTCTCC ACACTGGGAT ATGACTTGAC TTTCAGCACT
    GAGGTTCAAG AATTTGGTGT CTGTGAAGTC CGAGACCTAA AACCAAACGG TGCAAACATT
    CTGGTAACAG AAGAAAACAA AAAGGAGTAT GTTCATCTTG TGTGCCAGAT GAAGATGACA
    GGTGCAATAC GTAAGCAATT GGCTGCCTTC CTTGAGGGAT TTTATGAGAT AATCCCCAAA
    CGTCTGATCT CCATCTTCAC TGAGCAGGAA TTGGAACTGC TTATCTCTGG CCTTCCCACC
    ATAGATATTG ATGACCTAAA ATCAAACACT GAGTATCACA AATACCAGTC TAACTCCATC
    CAGATTCAGT GGTTCTGGAG AGCGTTGCGC TCATTCGATC AAGCTGACCG GGCAAAGTTC
    CTTCAGTTTG TTACTGGAAC TTCAAAGGTC CCATTACAGG GTTTTGCTGC GCTGGAAGGA
    ATGAACGGCA TCCAGAAATT CCAAATCCAC AGAGATGATA GATCTACAGA CAGGCTGCCT
    TCTGCGCACA CGTGCTTCAA CCAGTTGGAC CTTCCAGCCT ATGAGAGTTA CGAGAAGCTA
    CGTCACATGC TGCTGTTAGC CATCCAGGAG TGCTCTGAGG GCTTTGGCCT TGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748037.1
    CDS671..13747
    Translation

    Target ORF information:

    RefSeq Version XM_031892177.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892177.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    ATGAAAGTGG ACAGAACGAA GCTGAAGAAA ACGCCAACAG AGGCGCCTGC AGATTGCAGG 
    TCTCTGATAG ACAAACTGAA AGTCTGCAGT GATGAACAGC TTCTGCTGGA ACTACAGCAA
    ATCAAAACAT GGAACATTGG AAAGTGCGAG CTGTATCATT GGGTAGACTT GTTAGATCGC
    TTTGATGGCA TTCTAGCAGA TGCTGGACAA ACGGTAGAGA ACATGTCCTG GATGCTGGTG
    TGCGACAGAC CTGAGAGGGA ACAGCTAAAG GCTCTTCTGC TAGCAGTGTT AAACTTCACT
    GCTTTGCTTA TTGAGTACAG CTTCTCACGG CACCTGTACA GTTCCATCGA GCACCTTACA
    ACTCTGCTTG CTTCTTCTGA TATGCAAGTG GTCCTGGCTG TGCTAAATCT TCTCTACGTT
    TTTAGTAAGA GGTCCAACTA CATCACACGG CTAGCCTCTG ACAAGAGAAC ACCTTTACTG
    TCAAGGCTTC AGCACCTTGC AGAGAGCTGG GGAGGGAAAG AGAATGGCTT TGGACTTGCT
    GAATGTTGTA AAGATATGCA CATGTCGAAA TACCCACCAA GCGCCACAAC TTTACATTTT
    GAGTTTTATG CAGAACCTGG ATCCGAGGTG AAAGTTGAAA AAAGGGCATC CAGCAACACA
    CTACATTATA TACACATTGA ACAATTAGAT AAGATTTCTG AAAGTCCCTC TGAGATAATG
    GAATCTCTCA CCAAAATGTA CAGCATTCCC AAGGATAAAC AGATGCTTTT GTTTACACAC
    ATACGACTAG CACATGGCTT TTCTAATCAC AAGAAGCGAC TACAAGCTGT GCAGGCTAGA
    CTGCATGCCA TCTCTATACT AGTATATTCT AATGCCTTGC AAGAGTCTGC AAACAGCATC
    CTCTACAATG GTTTGATAGA GGAGTTAGTG GACGTGCTCC AAATAACAGA CAAGCAACTT
    ATGGAAATTA AAGCTGCATC ACTAAGGACC TTGACCTCTA TTGTACACTT AGAAAGAACC
    CCAAAGCTGA GCAGCATCAT TGACTGCACT GGGACTGCCT CTTACCATGG TTTCTTACCA
    GTCTTAGTCC GAAACTGCAT TCAAGCAATG ATAGATCCAA CCATGGAGCC ATACCCTCAC
    CAGTTTGCAA CAGCTCTTTT TTCCTTCCTG TATCACCTCG CCAGTTACGA TGCTGGTGGA
    GAAGCTCTTG TTTCCTGTGG AATGATGGAA GCGCTTCTTA AGGTTATTAA ATTCCTTGGA
    GATGAACAGG ACCAGATTAC GTTTGTGACG CGAGCTGTGA GGGTTGTTGA CCTTATTACA
    AACTTAGATA TGGCAGCATT TCAGTCCCAC AGTGGGTTGA CCATTTTTAT TTACCGACTA
    GAGCATGAAG TTGACCTATG CAGAAAAGAG TGCCCTTTTG TTATCAAGCC AAAAATACAG
    CGTCCCACCA ATGTTCAGGA AGGGGAAGAG ATGGAGACAG ATATGGAAGT GTCAGATGTT
    GCTATGGAGA GCTGTCCTGG TCCTTCGACC TCCACCGATC ACCGGCCTTC CCTGAGCACA
    AGTAGTAGCT CCAGTAGTAT AGTGGTGTCA CGAACAGGTG TGCAGTGTAT TCCTCAAAGG
    GCTGCTCTAC TAAAATCAAT GCTTAATTTC TTGAAAAAAG CAATCCAAGA TCCAGCTTTC
    TCAGATGGCA TCCGTCATGT TATGGATGGA ACTCTTCCTA CATCATTGAA ACACATAATC
    AGCAATGCTG AATACTATGG CCCTTCTTTG TTCCTGCTTG CTACTGAAGT GGTGACAGTT
    TTTGTTTTCC AAGAGCCCTC ACTGCTTTCT TCTCTTCAGG ACAACGGCTT AACAGATGTG
    ATGCTCCACG CTCTCCTTAT AAAAGATGTT CCTGCTACCC GAGAAGTTCT TGGATCTCTT
    CCTAATGTCT TCAGTGCTCT CTGTTTAAAT GCCCGAGGTC TGCAGTCCTT TGTGCAGTGC
    CAACCCTTTG AGCGATTGTT CAAAGTGTTG CTCTCACCTG ATTACCTTCC TGCTATGAGG
    CGACGGAGAA GCTCAGACCC TCTAGGCGAT ACAGCCTCTA ACCTGGGCAG TGCTGTTGAT
    GAACTGATGA GGCACCAGCC CACGTTAAAA ACAGACGCTA CAACTGCCAT CATTAAGCTC
    TTAGAAGAGA TATGTAACCT TGGTAGGGAC CCAAAATACA TCTGTCAGAA GCCATCTATT
    CAGAAAGCTG ATGGCACGGC TACTGCTCCT CCACCACGTT CCAACCATGC AGCAGAGGAG
    GCTTCTAGTG AGGATGAAGA GGAGGAGGAG GTACAAGCAA TGCAGAGTTT CAGCACAAAT
    CAGCAAAGTG AAGTTGAACC CAGCCAGCAT GTTGTTGGCA CAGAGGAGAG GATTCCTATT
    CCCCTAATGG ATTATATTCT CAATGTGATG AAATTTGTGG AATCAATTCT AAGTAACAAC
    ACAACTGATG ATCACTGCCA GGAATTTGTC AGTCAGAAGG GGCTCCTTCC ACTTGTCACC
    ATCCTTGGGC TTCCCAACCT ACCTATTGAC TTTCCTACCT CTGCTGCTTG CCAAGCTGTG
    GCAGGTGTCT GCAAATCCAT TTTGACTCTT TCTCATGAGC CAAAAGTTCT CCAAGAAGGA
    CTTCTTCAGC TGGATTCAAT TCTTTCATCT CTTGATCCAC TTCATCGGCC CATTGAGGCT
    CCTGGTGGCT CAGTACTGCT TCGAGAGTTG GCATGTGCTG GAAATGTGGC AGATGCTACT
    TTGTCTGCTC AAGCAACTCC ATTATTACAC GCTCTCACTG CAGCCCATGC CTACATCATG
    ATGTTTGTGC ATACATGCAG GGTTGGACAG AGTGAAATCC GTGCCATCTC TGTGAACCAG
    TGGGGATCAC AGCTGGGTCT CAATGTTTTG AACAAGTTGA GCCAGCTCTA CTGCTCCCTT
    GTATGGGAAA GTACAGTTTT GCTGTCACTT TGTACTCCTA ACAGTTTGCC TCCTGGGTGT
    GAGTTTGGCC AAGCAGATAT GCAGAAATTA GCCCCAAAGG AGGAAAAGGC AACCACTGTA
    TTGCCCCAAG GAACCAAAAG ACCTGAAAGT GAGGTTGAAG GCACAGTTGT CAGTGAGGAA
    GCTTCAGGCT TACTTGAAGG AATTGGACTG GATGGAGATA CTTTAGCTCC AATGGAAACT
    GATGAACCCA GCACTTCTGA TGCAAAGGGC AAACCGAAAA TAACCCCAGC TATGGCGGCC
    AGAATCAAGC AAATCAAACC ATTACTATCA GCTTCTTCTA GGTTGGGCCG GGCTTTGGCA
    GAGCTGTTTG GGTTGCTTGT CAAACTGTGT GTAGGTTCCC CTGTTCGGCA AAGGAGAAGT
    CACCATGCTG CCAGCACTAC CACAGCCCCT ACACCAGCTG CTCGCTCCAC AGCCTCTGCC
    CTCACCAAAC TGCTCACAAA AGGGCTGTCT TGGCAGCCTC CACCCTATAC CCCTACTCCT
    AGGTTCAGAT TGACTTTCTT TATCTGCTCC GTTGGGTTCA CATCCCCCAT GCTGTTTGAT
    GAAAGGAAAT ACCCATATCA CCTCATGCTG CAGAAATTCC TTTGCTCTGG TGGGCACAAT
    GCACTCTTTG AGACTTTTAA TTGGGCACTT TCAATGGGTG GAAGAGTTCC TGTTGCTGAG
    GGCCTGGAAC ACAGTGATCT TCCTGATGGT ACTGGAGAGT TTCTAGATGC TTGGCTTATG
    TTAGTGGAAA AGATGGTGAA TCCTACCACT GTGCTGGAAT CTCCTCATTC ACTTCCAACT
    ACAAAGTTAC CTGGAGGGCA GAATAATCCA CAGTTCAGTG CTTTACGCTT CCTTGTAGTC
    ACACAAAAGG CAGCTTTTAC CTGTATCAAA AACCTTTGGA ATCGGAAACC CCTGAAAGTA
    TATGGAGGGC GTATGGCAGA ATCAATGCTT GCAATCTTAT GTCATATACT TCGCGGTGAG
    CCAGTTATCC GTGAGCGTTT GAGCAAGGAG CGAGAGGGGA GCCGTGAAGA GGAGGGGCAG
    CAGGATGAGA GTGGAGCAAG ACGGGAGCCT CAAGTGAACC AACAGCAACT TCAGCAGCTG
    ATGGATATGG GTTTCACAAG GGAGCATGCT CTGGAAGCTC TGCTTAACAC GAGTACTATG
    GAGCAGGCCA CTGAATATCT CCTCACCCAC CCTCCACCTC TTATTGGCGG AGTTGTACGG
    GACTTAAGTA TGTCAGAGGA AGATCAGATG CTGAGAGCCA TTGCCATGTC GCTGGGACAA
    GATATCCCTA TGGAACAGAG GGCAGAACTG CCCGAGGAAA CTGCTCGGCG GAAAGAAGAG
    GAGGAACGCA AGGCACGAGA AAAACAAGAG GAAGAGGAAG CTAAATGCTT GGAAAAGTTT
    CAAGATGCAG AACCCCTGGA ACAGGATGAG CTTCAAGGAT TCACAGACAC CATGCTCCCT
    GGCTGCTTCC ACTTGCTTGA TGAGCTGCCA GACACAGTCT ATAGAGTGTG TGATCTTATA
    ATGACTGCCA TAAAGCGCAA TGGGGCAGAC TATCGAGACA TGATTCTAAA ACAAGTGGTT
    AACCAGGTAT GGGAAGCCGC TGATGTTCTT ATTAAAGCTG CACTGCCGCT GACCACAAGT
    GACACAAAAA CTGTGTCTGA GTGGATCAGC CAAATGGCTA CTCTTCCTCA AGCTTCTAAC
    TTGGCCACTC GCATCCTTTT GCTCACACTG CTGTTTGAGG AGCTAAAACT GCCATGTGCT
    AGAGTTGTTG AAACAAGTGG AATACTTAAC GTACTAATTA AGCTGCTTGA AGTGGTTCAG
    CCATGTTTGC AGGCAGCCAA GGAGCAAAAG GAAGTTCAGA CACCAAAGTG GATTACACCA
    GTCCTCCTCT TAATAGATTT TTATGAGAAG ACCTCAATTT CTTCTAAAAG AAGAATGCAA
    ATGAACAGGT ACCTTCAAGC TAATGGAAAC AACTGGCGAT GGTTTGATGA TCGGTCTGGC
    CGTTGGTGCA GCTACAGTGC TAGTAACAAC AGCACTATTG ATGCAGCCTG GAAAGCAGGA
    GAAACATGTG TGCGATTTAC AGCTGGCCGT CGAAGATATA CTGTGCAGTT TACCACAATG
    GTTCAGGTAA ATGAAGAGAC CGGCAATAGA CGACCTGTCA TGCTGACTCT CTTGCGTGTT
    CCGAGACTTT TAAAGAGTGG AAAAAATGGA AATGGACAGG AACTTGAAAA GACACTTGAG
    GAGGCAAAGG AGGTTGAAGC AAAGCAAAAA GACGAAAAAG TGCCTGAGCC CATTCCAGTT
    CCTGAAACCT CTTCCACTGA GAAAGATGCA CCAACTGAAG AGCCCAAAGT TGGAGACATA
    GTAATTCAGG GTCTGGCTGA AGAAATGGTG ACCGTCCTTA TACGTTCCTG CGTCGGCATG
    CTTGGTGTTC CTGTTGATCC AGATACTCTT CATGCAACCT TGCGTCTTTG CCTGCGTCTC
    ACACGTGACC ACAAATATGC CATGATGTTT GCAGAGCTTA AGAGCACTCG AATGATCCTC
    AGCTTAACTC AAAGCTCTGG ATTCAATGGC TTTACTCCCC TTGTGACGCT CCTATTTAGG
    CACATCATAG AGGATCCTTG CACCCTCCAG CACACCATGG AAAAGGTTGT ACGGTCTGCA
    GCAACTAGTG GGGCAGGGAG TACCACATCT GGGGTTGTGT CTGGAAGCCT GGGTTCGCGT
    GAAATCAACT ATATACTTAG AGTACTGGGT CCAGCAGCTT GTCGAAACCC TGACATTTTC
    ACTGAAGTGG CAAACAGTTG CATTCGCATT GCTCTCCCTG CACCTCGTGG ATCTGGAACT
    GCCTCTGATG ATGAGTTTGA GAACCTTCGG ATCAAAGGTC CTAATGCAGT TCAGTTGGTT
    AAAACCACCC CTATAAAGCC CTCCACCTTG CCAGTCATCC CTGATACAAT TAAGGAAGTA
    ATCTATGACA TGCTGAATGC ACTGGCAGCT TACCACGCCC CTGAGGAAGT ATCTGAAAAG
    TCTGAGCAAA AACCTGTTGG CATGAACCAG GAAGTCAGCC AGATCCTACA GGACATGGGT
    GATGAGGTTT TCCAGCAATA TCGGGACTTA ACCAGACAGG GAAGCGACTT TGATGGTCAG
    ACCAGTTTTG CCATCAACGC GCGAGTCTTT ACTGCTGACA GCACAGCTGC CGAAACACCT
    CAGTCTGGTA CACCTCAAGG TGAAACTGCC TTAACACCTG AAGAAAACCG AGAAGGGTTA
    AAGAAAGATA AGGAAGGAGA CCGTAGTTCA GAAGATGGAA AGCAGAAGGC AAAAGGTAGT
    AAGCCTTTGC TGCCTACCTC TTCCATTCTT CGACTACTTG CTGAACTTGT CAGGTCCTAT
    GTCGGAATAG CCTCGCTGAT CGCCACCTAC AATTACACTG CAGGACAATC TGAACTAATT
    AAAGAGGATT GCAGTGTATT GGCGTTTGTG CTGGATCACC TGCTACCCCA CACACATAAT
    GCAGAGGACA AGGACACACC AGCCCTAGCC CGCCTCTTCT TAGCCAGCCT TGCTGCAGCA
    GGAAGTGGCA CTGATGCCCA GGTTGCACTA GTGAATGAAG TAAAGGCAGC TCTTGGCAGA
    GCCCTAGCAA TGGCTGAGAG CACGGAGAAG CATGCTAGGT TGCAGGCTGT GATGTGCATT
    ATCAGTACCA TTATGGAGTC ATGTCCATCC ACCTCCAGCT TCTACAGCAC AGCAACCACC
    AAAACCCAGC ACAATGGCAT GAACAACATC ATCAGACTCT TTTTGAAAAA GGGGCTGGTT
    AGTGATTTAG CCAGGGTCCC TCACAGCCTT GATTTATCCA GTCCAAATAT GGCAAACACC
    GTGAATGCAG CACTGAAGCC ACTCGAGACA CTGTCTCGCA TTGTCAATCA ACCCAGCAGT
    TTGTTTGGAA GCAAAGGGAC TTCAGGAAAG AATAAATCTG AGGCAGATAC ACATGGAACA
    GCCCAGGATC CCAACAGTAA TCAGCCAGAT ACAGGTGAAA CAGGAACAGA AGTGCAGGAA
    GAGGACCCAG ATGCTTCTCA TGCTGAAGTA GCTGATGGAG ACATCATGGA TGGAGAGACA
    GAGACTGATG CTGTGGTTAT TGCTGGCCAG CCTGAAGTCC TTACTACACA AGAAATGCAG
    GTAGAGAATG AGCTTGAAGA TCTGATAGAT GAACTTCTTG AAAGGGATGG TGCTGCAGGT
    AACCGGGTCG GAGAAGATGA ATCACAGGAA GATGTGCTGA TGGATGAGCC TCCTACTAAC
    CTCAGCCAAG CCTCCACTCT TCAGGCTAAT CGAGAAGACT CTATGAACAT CTTAGACCCA
    GAGGATGAGG AAGAGCACAC CCAAGAAGAG GACAGCAGTG GCAGCAATGA AGATGAAGAT
    GACAGCCAGG AAGAGGAGGA GGAAGAGGAA GATGAGGACG ATCAGGATGA TGAGGAGGGT
    GAGGAAGGGG ATGAGGATGA TGATGATGAC TGTTCAGAGA TGGAACTGGA TGAGGATTAT
    CCAGACATGA ATGCCTCACC TCTTGTACGC TTTGAACGTT TTGACAGAGA AGATGATTTA
    ATCATAGAGT TTGATAACAT GTTCTCCAGT GCCACTGAGA TCCCTCCATC CCCTGGTAAC
    ATCCCTACTA CACACCCCCT AATGGTGCGA CACGCTGATC ACGGCTCCTT GACTCTTGGC
    AGTGGCACTT CCACTACTCG CCTGGCACAG GGCATCGGGC GCAGCCAGAG AACACTCCGA
    CAACTCACAG CCAACACCGG TCACACAATA CATGTTCATT ATCCTGGCAA CAGGCAGCCA
    AATCCACCTT TAATCCTCCA GAGGTTGCTG GGGCCCTCAG CCGCTGCTGA CATCTTGCAA
    CTAAGCAGCA GTCTTCCTCT GCAGAGTCGT GGCCGAGCAC GTCTACTTGT TGGCAACGAT
    GATGTCCACA TCATAGCACG CTCTGATGAT GAGATGCTTG ATGATTTTTT CCATGATCAG
    AGTACAGCCA CAAGTCAGGC TGGCACACTT TCCAGCATTC CAACTGCGCT GACCCGCTGG
    ACAGAAGAGT GTAAGGTCTT GGATGCTGAA AGCATGCATG ACTGTGTATC TGTGGTCAAA
    GTCCCAATCA TCAATCATTT GGAATCTTTG CGTGATGAGG AGCTTGAGGA GCGCAGGGAA
    AAAAGAAGGA AACAACTAGC AGAAGAGGAG GCCAAGATGG CAGAGAAATC CAAGGAGGAG
    AAGGAAGCAA AGGAGCAAAA TTCACAGGGT GCCAATTCAA AGCAGAATAC CCCATTTGAA
    CTGAGCCTTG CAGATCAAGA TGGAAGTGGT CGTCCTGAAA ATTTCCCAAC TGCACCATCT
    TCTGCAGAAG GAACTCCAAC AAGTCCTGAA ATTGTTCGTT TAGATCCCAC CCTAAGGGCT
    ACTGAGGCTA CTCTGGCAGA GCTGACTCAG GGTCTGCCTG CTGATACAGG CACGGAGGAT
    GTTGGGCAAC AACTCATGCC ATCTCACCAA GAGGACTCTG GACCAGGACG GCCAAGTGTA
    GAGGGGGATG CCACACAAAT GGAGCTATCG CCAGCACCCA CTATAACCTC CTTATCACCA
    GAGCGAGCAG AGGATTCAGA TGCTTTGACA GCTGTTAGTA GCCAACTAGA GGGCTCGCCT
    ATGGACACCA GCAGTCTTGC CTCTGGGCCC TTAGAGGATG GCGTAGGGGA GGCACCTGGA
    ATTGTTAATC CAGAGCAGCC TCTTGTGGTT GGCAGTAGCA TTGCTGTTGG ACAGCAACCA
    GTGGAACCAC CGACGACCTG TGAAACCCAA CCAGTAGTTT CTCAACACAG TGATAGCTCA
    CAAATTGCCC CTTCCTCTGA GTTGCCTTCC ACAAGAGAAA GTGCTGTGAC CTTGTTGGCT
    ACAGATTCTA GTGGTATCCT TGAAGAACCT TTGCCTTCTA CAAGCAGTGA AGAGGAAGAC
    CCATTAGCAG GCATAAATCT TCCAGAGGGT GTGGATCCTT CTTTCCTAGC GGCTCTTCCT
    GAAGACATCC GCAGAGAGGT GCTGCAGAAT CAGTTGGGCA TACGGCCGCC ATCGCGACCT
    CCACCCACTG CTGCCCCAGT TATTCCTCCT CCTGTGGTTG CTAATGCTGG TGTCACGGAG
    GTCAGCCCTG AGTTCTTGGC AGCGTTGCCA CCAGCCATTC AGGAAGAGGT CCTAGCACAG
    CAGCGAGCAG AACAGCAGAG GCGGGAACTT GCTCAGACCA CTACGTCTGA TACACCCATG
    GATCCTGTGA CTTTCATTCA GACGTTACCT TCTGATCTAC GTCGTAGTGT GTTAGAAGAC
    ATGGAGGACA GTGTTTTAGC TGTGATGCCA CCTGACATTG CTGCAGAGGC ACAGGCTCTA
    AGGCGAGAGC AAGAAGCTCG GCAGAGGCAA CTAATGCATG AACGACTGTT CGGGCACAGT
    AGCACATCTG CACTCTCTGC CATCCTTAGG AGTCCTGCTT TCACGAGTCG CTTGAGTGGA
    AACCGAGGTG TTCAGTACAC ACGGTTGGCT GTACAGAGAG GGAACACTTT CCAAATGGGA
    AGCAGCAGTA CACATACCAG ACCAACCGGT AGTAATGTTG ACAGCCTACT ACGATTACGA
    GGCCGACTAT TACTTGATCA TGAAGCCCTC TCCTGTCTGC TTGTCCTACT GTTTGTTGAT
    GAACCCAAGC TAAACACCAG TCGCTTGCAC CGTGTTCTAC GCAACCTCTG CTACCATGCA
    CAAACAAGGA ACTGGGTGAT TAGAAGCTTG TTGTCTATAC TGCAGCGTAG CAGTGAAAGT
    GAGCTTTGTA TAGAAAGCCC AAAGACTTCT ATTGGGGGCA CTGATAAACG GGGTGCAAAA
    ACAAGTCATG ACAACCGCCC TTTAGAATTT CTGCATAAGA TGGAATCGAA GAATTCCAAC
    CAGCTCTCCT GGCTTTCTGT ATCCATGGAC GCTGCTCTTG GCTGCCGTAC GAACATCTTC
    CAAATCCAGA GGTCCCTAGG ACGCAAGCAT ACAGAAAAGC ATTCAGCCTG TGGTTCTACT
    GTCCACATCC ATCCCCAAGC AGCCCCTGTA GTGTGTAGAC ATGTGCTTGA CACACTAATA
    CAGCTAGCTA AGGTGTTTCC CAGTCATTTC ACACAGCAGC GAACCAAAGA ATGTAGTGTG
    GAAAACAGTG ACAGCAGGGA GCGGAGTATA AAACTTACTT GTAGTCCGTG TCTTGCACCA
    TCTAGTAGTA TTGGAGCAAG CAACATCTCC ACAGATTTCT GGGACTTGCT AGTCAAGCTA
    GACAACATGA ATGTGAGCCG GAAGGGGAAA ACGTCTATAA AATCTGTGCC AGTGAGCTCT
    GGTTCTGAAG GCGAGAGCAA TCAGTGCAGC CTGGAGGCCT CCCCCTTAGG ACAGCTTATG
    AATATGCTGT CTCATTCTGT TATTCGCCGC AGTTCGCTGC TTACTGAAAA GCTGCTGAGA
    CTTCTCTCCC TTATCTCTAT TGCCCTTCCT GATAATAAGG CTTCTGAAAC AGCAGGCAAT
    CAGAGCAATT CTATTACCCC AGCACCTGCA CCTACGCCTG CAACTGCACC AGCCCCTGTG
    CCTGTACCTG CTGTAACAGT TCCAGTTGTT GTGAGCACAA ACATAGTGAC AACTGTTACA
    ACCAAAAGTG GAAAATCTCC TGCAAAATCA AGTGAAGTCA GCAATGCACC AGATAAGATG
    GCAGCTTCTG GCCTTACAGA GAAACAGCTG CAGTTGTCTG TTGAGGTTCT GACATCACAC
    TCGTGTTCAG AAGAAGGACT GGAGGATGCC GCCAATGTTC TACTTCAGCT TTCCAGAGGC
    GACCCCAGTA CACGGGACAC TGTACTGAAA CTTCTGCTTA GCGGAGCACA TCACTTGGGG
    TTTACTCTCT GCAAACAGAT AGGTACACTA CTAGCAGAGC TCCGGGAATA TAACCTAGAG
    CAGCAGCGTC GTGCACAGTG TGAAGTTCTT TCTCCAGATG GTATGCCTGA GGAGCAACCC
    CACAGCACAA AGCCAAAAGG CAAAATGCAG AGCAGGTTCG ACATTGCAGA GAATGTGGTG
    ATTGTGGCTT CCCAAAAGCG TCCACTGGGA GGAAGAGAGC TGCAGCTCCC CTCAATGTCT
    ATGCTGACGT CAAAGACTTC CACACAAAAA TTCTTTCTTC GAGTGCTGCA GGTTATCATC
    CAGCTTCGCG ATGACACTCG CAGAGCCAAC AAGAAGGCCA AGCAGACCAG CAGATTAGGC
    ACTGCCAGTC TGGCCTCGGC TAGCAGCATC CAAGCAGCTG TGCAGCAGCT GGAGGCTGAG
    GCTGATGCCA TTATACAAAT GGTACGTGAG GGTCAAAGGG CGCGGAGACA GCTGCAAACT
    GCAACGGCGG AGGGTTCTGC TCGCAGGGAG GAGCAGCCTA TGGATGTTGA CCAGCCTTCG
    CCTGCTACAC AGGAGCCTCA GTCAGTTGCT TCTGAAGGTT CTCAGGCTGC TGAAAAAAAA
    GAAGAGGAGA GGTTACCAGA GTTACCGCTG TTAAGTGAGC AGCTAAGTTT AGATGAGCTG
    TGGGACATGT TGGGAGAGTG CCTGAAGGAA CTTGAGGAAT CTCATGACCA GCATGCCGTA
    TTAGTGTTAC AGCCAGCCGT AGAAGCCTTT TTCCTTGTTC ATGCAACAGA GCGGGAGAGC
    AAGCCTCCTG TGCGGGACAC TCGGGAAAGC CAGTTATCTC ACATTAAGGA TGAACCACCT
    CCCCTGTCTC CTGCACCCCT CACTCCAGCC ACACCTTCTT CCTTGGATCC TTTCTTCTCT
    CGTGAACCTT CATCTATGCA CATTTCCTCT AGTTTGCCTC CTGACACTCA GAAGTTCCTA
    AGATTTGCTG AGACTCATCG GACTGTATTA AATCAGATCC TTCGCCAGTC CACCACACAC
    CTAGCTGATG GGCCCTTTGC TGTGCTTGTA GACTATATCA GAGTCCTTGA CTTTGATGTT
    AAGCGCAAAT ATTTCCGACA GGAGTTGGAG CGACTGGATG AAGGGCTAAG AAAAGAGGAT
    ATGGCAGTGC ATGTGCGGCG AGATCATGTG TTTGAGGATT CCTACCGGGA GCTGCACCGC
    AAATCCCCTG AAGAAATGAA GAATAGATTG TACATAGTAT TTGAAGGAGA AGAAGGGCAG
    GATGCAGGTG GGCTGCTTCG GGAGTGGTAC ATGATTATCT CCAGGGAGAT GTTCAATCCC
    ATGTATGCTT TGTTCCGCAC TTCCCCAGGT GACCGCGTCA CCTACACTAT TAATCCTTCC
    TCCCACTGTA ACCCTAACCA CCTCAGCTAC TTCAAGTTTG TGGGCCGGAT AGTTGCCAAA
    GCGGTTTATG ACAACCGGCT ACTGGAGTGT TACTTCACTA GGTCCTTCTA CAAGCATATA
    TTGGGCAAGT CTGTCAGATA CACAGACATG GAGAGTGAAG ATTATCACTT CTATCAGGGT
    CTGGTGTACT TGCTTGAAAA TGATGTCTCC ACACTGGGAT ATGACTTGAC TTTCAGCACT
    GAGGTTCAAG AATTTGGTGT CTGTGAAGTC CGAGACCTAA AACCAAACGG TGCAAACATT
    CTGGTAACAG AAGAAAACAA AAAGGAGTAT GTTCATCTTG TGTGCCAGAT GAAGATGACA
    GGTGCAATAC GTAAGCAATT GGCTGCCTTC CTTGAGGGAT TTTATGAGAT AATCCCCAAA
    CGTCTGATCT CCATCTTCAC TGAGCAGGAA TTGGAACTGC TTATCTCTGG CCTTCCCACC
    ATAGATATTG ATGACCTAAA ATCAAACACT GAGTATCACA AATACCAGTC TAACTCCATC
    CAGATTCAGT GGTTCTGGAG AGCGTTGCGC TCATTCGATC AAGCTGACCG GGCAAAGTTC
    CTTCAGTTTG TTACTGGAAC TTCAAAGGTC CCATTACAGG GTTTTGCTGC GCTGGAAGGA
    ATGAACGGCA TCCAGAAATT CCAAATCCAC AGAGATGATA GATCTACAGA CAGGCTGCCT
    TCTGCGCACA CGTGCTTCAA CCAGTTGGAC CTTCCAGCCT ATGAGAGTTA CGAGAAGCTA
    CGTCACATGC TGCTGTTAGC CATCCAGGAG TGCTCTGAGG GCTTTGGCCT TGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.