HUWE1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_005261965.3	XP_005262022.1	E3 ubiquitin-protein ligase HUWE1 isoform X12
XM_017029205.1	XP_016884694.1	E3 ubiquitin-protein ligase HUWE1 isoform X11
XM_017029191.1	XP_016884680.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_017029193.1	XP_016884682.1	E3 ubiquitin-protein ligase HUWE1 isoform X3
XM_017029194.1	XP_016884683.1	E3 ubiquitin-protein ligase HUWE1 isoform X4
XM_017029195.1	XP_016884684.1	E3 ubiquitin-protein ligase HUWE1 isoform X5
XM_017029196.1	XP_016884685.1	E3 ubiquitin-protein ligase HUWE1 isoform X6
XM_017029199.1	XP_016884688.1	E3 ubiquitin-protein ligase HUWE1 isoform X8
XM_017029201.1	XP_016884690.1	E3 ubiquitin-protein ligase HUWE1 isoform X8
XM_017029203.1	XP_016884692.1	E3 ubiquitin-protein ligase HUWE1 isoform X8
XM_011530747.1	XP_011529049.1	E3 ubiquitin-protein ligase HUWE1 isoform X9
XM_017029200.1	XP_016884689.1	E3 ubiquitin-protein ligase HUWE1 isoform X8
XM_005261965.4	XP_005262022.1	E3 ubiquitin-protein ligase HUWE1 isoform X11
XM_017029192.1	XP_016884681.1	E3 ubiquitin-protein ligase HUWE1 isoform X2
XM_017029202.1	XP_016884691.1	E3 ubiquitin-protein ligase HUWE1 isoform X8
XM_017029198.1	XP_016884687.1	E3 ubiquitin-protein ligase HUWE1 isoform X8
XM_017029204.1	XP_016884693.1	E3 ubiquitin-protein ligase HUWE1 isoform X10
XM_017029206.1	XP_016884695.1	E3 ubiquitin-protein ligase HUWE1 isoform X12
NM_031407.6	NP_113584.3	E3 ubiquitin-protein ligase HUWE1
XM_017029197.1	XP_016884686.1	E3 ubiquitin-protein ligase HUWE1 isoform X7
XM_017029198.2	XP_016884687.1	E3 ubiquitin-protein ligase HUWE1 isoform X8
XM_024452322.1	XP_024308090.1	E3 ubiquitin-protein ligase HUWE1 isoform X9
XM_011530751.2	XP_011529053.1	E3 ubiquitin-protein ligase HUWE1 isoform X9
NM_031407.7	NP_113584.3	E3 ubiquitin-protein ligase HUWE1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23451	NM_031407.7 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu23451	XM_005261965.4 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu63653	XM_011530751.2 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu63653	XM_024452322.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu63653	XM_011530747.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99112	XM_017029191.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99113	XM_017029192.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99114	XM_017029193.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99115	XM_017029194.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99116	XM_017029195.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99117	XM_017029196.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99118	XM_017029197.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99119	XM_017029198.2 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99119	XM_017029199.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99119	XM_017029200.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99119	XM_017029201.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99119	XM_017029203.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99119	XM_017029202.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99120	XM_017029204.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99121	XM_017029205.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99122	XM_017029206.1 Latest version!	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu23451	XM_005261965.3	Homo sapiens HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu99119	XM_017029198.1	Homo sapiens HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu23451	NM_031407.6	Homo sapiens HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

HUWE1 ( NM_031407.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_113584.3 Homo sapiens HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), mRNA.

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CloneID	OHu23451
Clone ID Related Accession (Same CDS sequence)	XM_005261965.3 , XM_005261965.4 , NM_031407.6 , NM_031407.7
Accession Version	NM_031407.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG334536.1, DQ097177.1, BX323845.7, AY772009.1, AC231658.3, AB002310.3 and R60532.1. This sequence is a reference standard in the RefSeqGene project. On Aug 20, 2013 this sequence version replaced NM_031407.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ097177.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAATCAGC AAACAGTATC 
TTGTATAATG GCTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACGGA TAAGCAGCTT 
ATGGAGATTA AAGCAGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT 
CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCA 
GTGCTTGTAA GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATACCCTCAC 
CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC 
GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGATA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT 
GAGCATGAAG TAGATTTGTG CCGAAAAGAA TGTCCGTTTG TGATCAAGCC AAAGATCCAG 
AGACCCAATA CTACACAAGA AGGAGAGGAA ATGGAAACTG ATATGGATGG AGTCCAGTGT 
ATTCCACAAC GAGCAGCACT TCTGAAATCC ATGTTGAATT TCCTCAAGAA GGCCATCCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTCTGCC TACCTCCCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGCCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAACCA TCACTGCTCT CCTCACTCCA GGACAATGGA 
TTGACAGATG TCATGCTGCA TGCACTGCTT ATCAAAGATG TTCCTGCTAC CCGTGAAGTC 
CTTGGCTCCC TCCCAAATGT ATTCAGTGCA CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTTCAGT GTCAGCCTTT TGAACGCCTC TTCAAAGTTC TTCTGTCTCC AGATTACCTC 
CCAGCCATGC GGAGGAGGAG AAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTCG ATGAGCTCAT GAGACATCAG CCCACCCTTA AAACAGATGC AACGACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGGG ACCCCAAATA CATCTGTCAG 
AAGCCATCAA TCCAGAAGGC AGATGGCACT GCCACTGCTC CTCCCCCAAG GTCTAATCAT 
GCCGCAGAAG AAGCCTCTAG TGAGGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTA CCCAGCAAAA TGAAACTGAG CCTAATCAGC AGGTTGTTGG TACAGAGGAA 
CGTATTCCTA TTCCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
CTGAGCAACA ATACAACAGA TGACCACTGC CAGGAATTTG TGAATCAGAA AGGACTGTTG 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACAC TGTCACATGA ACCCAAAGTC 
CTTCAAGAGG GTCTCCTTCA GTTGGACTCC ATCCTCTCCT CCCTGGAGCC CTTACACCGC 
CCCATTGAAT CCCCTGGGGG CTCAGTGTTG TTGCGAGAAC TGGCTTGCGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC CCAGGCCACA CCTCTGCTGC ATGCACTCAC TGCTGCCCAT 
GCCTACATCA TGATGTTTGT TCATACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCCATC 
TCCGTAAACC AGTGGGGCTC TCAATTGGGT CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA 
TACTGTTCCC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TGTGTACCCC AAACAGCCTA 
CCATCTGGGT GTGAATTTGG CCAGGCAGAT ATGCAGAAAC TGGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGGGAAC AGGATGGAGC AGCTGGAAGT 
ATGGATGCTT CTACCCAGGG CTTATTAGAA GGCATTGGGC TAGATGGTGA CACATTGGCT 
CCCATGGAGA CAGATGAACC TACTGCTTCA GACTCTAAGG GCAAATCTAA AATCACACCA 
GCAATGGCTG CCAGAATTAA GCAAATCAAG CCTTTGTTAT CAGCTTCCTC CAGATTAGGC 
CGAGCACTTG CTGAGCTATT TGGACTTCTT GTTAAACTTT GTGTGGGATC TCCTGTCCGC 
CAGAGAAGGA GCCATCATGC TGCCAGCACC ACTACAGCAC CGACACCTGC CGCGCGATCA 
ACAGCCTCAG CTCTCACTAA GCTCTTGACT AAGGGGTTAT CTTGGCAGCC CCCACCATAT 
ACACCTACTC CCCGATTCAG GCTGACATTC TTCATCTGTT CAGTTGGTTT CACATCCCCA 
ATGCTGTTTG ATGAGAGGAA GTATCCCTAC CACCTCATGC TGCAAAAATT TCTCTGCTCC 
GGAGGCCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TGTCCATGGG AGGTAAAGTT 
CCTGTTTCTG AGGGATTGGA ACACTCAGAC TTGCCTGATG GCACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTGGTGGA GAAGATGGTG AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGCTGCCTG CCAAATTGCC TGGAGGTGTC CAGAACTTTC CCCAGTTCAG TGCACTGCGC 
TTCCTTGTGG TAACTCAGAA AGCAGCCTTT ACTTGCATCA AAAACTTATG GAACCGGAAA 
CCCCTGAAGG TATATGGTGG ACGAATGGCT GAATCGATGC TGGCCATTCT ATGCCACATC 
CTCCGAGGAG AACCTGTGAT TCGAGAGAGA CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA 
GAAGAGGATA CAGGGCAAGA GGAAGGTGGC TCCCGCCGGG AACCTCAAGT CAACCAGCAA 
CAACTGCAAC AGCTCATGGA CATGGGCTTC ACAAGGGAAC ATGCAATGGA GGCACTGTTG 
AACACCAGCA CCATGGAGCA GGCCACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATG 
GGAGGAGTTG TTCGGGATCT CAGCATGTCT GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA 
TGTCTAGAGA AGTTCCAGGA TGCTGACCCG TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACTATGT TGCCAGGCTG CTTCCACCTT CTTGATGAGC TGCCAGACAC AGTATACCGT 
GTGTGTGACC TGATCATGAC AGCAATCAAA CGTAATGGAG CAGATTATCG TGACATGATT 
CTGAAGCAAG TAGTCAATCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCCCTGACAA CAAGTGACAC AAAAACCGTG TCAGAGTGGA TAAGTCAGAT GGCCACACTG 
CCCCAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CGCTACTTTT TGAGGAGTTG 
AAGCTACCTT GTGCTTGGGT GGTTGAATCA AGTGGCATCC TTAATGTCCT AATCAAACTC 
TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA GCCAAGGAGC AGAAGGAAGT CCAGACCCCA 
AAGTGGATCA CACCAGTGTT GCTCCTGATT GATTTCTATG AAAAGACAGC CATCTCCTCA 
AAAAGGAGAG CCCAGATGAC TAAGTACCTG CAATCCAACA GCAACAACTG GCGCTGGTTT 
GATGATCGCT CTGGGCGTTG GTGTAGTTAC AGTGCAAGCA ACAATAGCAC TATTGATTCT 
GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA TTCACTGCAG GCCGAAGAAG ATACACGGTC 
CAATTCACTA CAATGGTGCA GGTTAATGAG GAAACAGGGA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTACCTCG GCTGAATAAA AATTCAAAAA ACAGCAATGG ACAGGAACTA 
GAGAAGACGC TGGAAGAAAG CAAAGAAATG GATATCAAAC GTAAAGAAAA TAAAGGCAAT 
GATACCCCTT TGGCCCTAGA GAGTACAAAC ACTGAAAAGG AGACAAGCCT GGAGGAAACA 
AAAATCGGGG AGATCCTGAT CCAGGGCTTG ACAGAAGATA TGGTGACTGT TTTAATCCGG 
GCCTGCGTGA GCATGCTGGG AGTCCCTGTG GACCCAGATA CTTTGCATGC CACCCTTCGT 
CTCTGTCTGA GGCTCACCCG GGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACCCGCATGA TCTTGAATTT GACCCAGAGC TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC 
ACCCTTCTCT TAAGACACAT CATTGAGGAC CCCTGTACCC TTCGTCATAC CATGGAAAAG 
GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT GGTAGCACTA CCTCTGGTGT TGTGTCTGGC 
AGCCTCGGCT CTCGGGAGAT CAACTACATC CTTCGTGTCC TTGGGCCAGC CGCATGCCGC 
AATCCAGACA TATTCACAGA AGTGGCCAAC TGCTGTATCC GCATCGCCCT TCCTGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGCCCTAAT 
GCTGTACAGC TGGTGAAGAC CACCCCTTTG AAGCCCTCAC CTCTGCCTGT CATCCCTGAT 
ACTATCAAGG AAGTGATCTA TGATATGCTG AATGCTCTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGATA AATCTGATCC TAAACCTGGG GTTATGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGATGATGT ATACCAGCAG TACCGGTCAC TTACGCGTCA GAGCAGTGAC 
TTTGATACGC AGTCAGGTTT TTCCATTAAT AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC 
ACTGAGACTT CCGCATCTGG GACCTCCCAA GGAGAGGCTT CAACTCCAGA GGAGTCTCGA 
GATGGGAAGA AAGATAAAGA AGGGGACCGG GCCTCTGAGG AAGGCAAACA GAAAGGCAAG 
GGCAGCAAAC CTTTAATGCC TACCTCCACT ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG 
TCCTATGTTG GTATTGCTAC CCTGATTGCC AACTACAGCT ACACTGTGGG CCAGTCTGAA 
CTGATCAAAG AGGACTGCAG TGTGCTAGCT TTTGTTCTGG ACCACCTGCT CCCACATACC 
CAGAATGCAG AAGACAAGGA CACCCCTGCC TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT 
GCTGCAGGGA GTGGCACAGA TGCCCAGGTG GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT 
GGACGGGCAC TGGCTATGGC TGAGAGTACA GAGAAACATG CCAGGCTTCA GGCAGTGATG 
TGTATCATCA GTACTATCAT GGAGTCCTGC CCCTCCACCT CCAGCTTCTA CAGCAGTGCC 
ACAGCGAAGA CCCAGCACAA TGGCATGAAC AACATCATTC GGCTTTTCCT GAAGAAGGGA 
CTGGTTAATG ACCTGGCCAG AGTACCTCAC AGCTTAGACC TGTCCAGTCC CAACATGGCC 
AACACAGTCA ATGCTGCTCT GAAGCCTTTG GAAACACTTT CCCGGATTGT GAACCAGCCC 
AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT AGCAAGAACA AGTCTGAGCA GGATGCCCAA 
GGAGCCTCTC AAGATTCCAG TAGCAACCAG CAGGACCCAG GCGAGCCTGG GGAAGCAGAA 
GTGCAGGAGG AGGATCATGA TGTCACTCAG ACAGAGGTGG CAGATGGGGA TATCATGGAT 
GGGGAGGCTG AAACCGACTC AGTGGTGATT GCTGGGCAGC CTGAGGTGCT CAGTTCACAA 
GAGATGCAGG TTGAGAATGA GCTGGAGGAC CTGATAGATG AGTTGCTTGA GAGGGATGGC 
GGATCTGGGA ACAGTACAAT TATAGTGAGC AGAAGTGGAG AGGATGAATC ACAAGAGGAC 
GTGCTGATGG ATGAAGCTCC TTCCAACCTC AGCCAAGCTT CCACCTTGCA GGCCAACCGA 
GAAGATTCCA TGAATATCCT GGACCCTGAG GATGAGGAGG AGCACACTCA GGAAGAGGAC 
AGCAGTGGCA GTAACGAGGA TGAGGATGAT AGTCAGGATG AAGAGGAGGA GGAGGAGGAA 
GATGAGGAAG ATGATCAGGA GGATGATGAA GGTGAAGAGG GAGATGAAGA CGATGACGAC 
GATGGCTCTG AGATGGAATT GGATGAGGAT TATCCTGATA TGAACGCTTC TCCCTTGGTC 
CGATTTGAGC GCTTTGACCG GGAGGATGAT CTCATCATTG AGTTTGACAA CATGTTCTCC 
AGTGCTACAG ACATCCCCCC ATCCCCAGGA AATATCCCTA CCACCCATCC ACTGATGGTG 
CGCCATGCAG ACCACAGTTC TCTGACACTG GGCAGTGGCT CTTCAACAAC TCGTCTCACC 
CAGGGCATCG GGCGCAGTCA GAGGACCCTA AGGCAGCTGA CGGCCAATAC TGGCCACACC 
ATTCATGTTC ACTACCCTGG GAATCGCCAG CCCAACCCTC CTCTTATACT GCAGAGGTTG 
CTTGGTCCCT CAGCTGCTGC TGACATCCTT CAGCTGAGCA GCAGCCTTCC CCTACAAAGC 
CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC GATGACGTCC ACATCATCGC CCGTTCTGAT 
GATGAGCTGC TGGATGACTT TTTCCATGAT CAGAGCACAG CTACCAGCCA AGCAGGAACC 
CTGTCCAGCA TCCCCACAGC CCTGACCCGC TGGACAGAAG AATGCAAAGT TCTCGATGCT 
GAGAGCATGC ATGACTGTGT TTCAGTGGTT AAAGTGTCCA TTGTCAATCA CCTGGAATTC 
CTGAGGGATG AGGAGCTGGA AGAAAGGCGA GAGAAGCGCA GGAAACAACT GGCTGAGGAA 
GAAACAAAGA TAACTGATAA AGGCAAAGAA GATAAGGAGA ACAGGGATCA GAGTGCACAG 
TGTACTGCAT CTAAGTCAAA TGACTCCACT GAACAGAATC TCTCAGATGG GACGCCTATG 
CCTGACAGCT ACCCAACAAC CCCATCTTCA ACTGATGCAG CTACATCTGA GTCCAAGGAG 
ACCCTTGGCA CTCTGCAATC CTCACAACAG CAACCAACAC TCCCAACCCC ACCAGCTTTG 
GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA GCTGGAGAAG GGGGCAGCTC TACACAGCTA 
TTGATGCCTG TAGAGCCAGA GGAATTGGGT CCCACAAGGC CAAGTGGGGA AGCAGAAACA 
ACTCAGATGG AGTTATCCCC AGCTCCCACT ATAACCTCAC TTTCCCCAGA GAGAGCTGAG 
GATTCTGATG CACTGACGGC TGTCAGCAGT CAGCTAGAAG GCTCTCCTAT GGATACAAGC 
AGCCTGGCTT CCTGTACCTT AGAGGAGGCT GTGGGTGACA CTTCAGCAGC TGGCAGTTCT 
GAGCAGCCCA GAGCAGGCAG CTCCACTCCT GGGGATGCCC CACCAGCTGT GGCGGAAGTG 
CAAGGCAGGA GTGATGGGTC AGGGGAATCT GCCCAGCCAC CTGAGGACAG CTCCCCACCT 
GCATCCTCTG AGAGCTCTTC CACCAGAGAT TCTGCCGTGG CCATTTCTGG AGCAGATTCC 
CGAGGAATCC TAGAAGAGCC GTTGCCTTCA ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG 
GGTATCAGTC TCCCTGAAGG TGTGGACCCC TCTTTTCTGG CTGCCCTGCC TGATGACATC 
CGTCGGGAAG TTCTACAGAA CCAGCTAGGC ATTCGTCCAC CAACCCGGAC TGCCCCCTCC 
ACAAATAGCT CAGCGCCTGC AGTGGTGGGG AATCCTGGTG TGACTGAAGT GAGCCCTGAG 
TTTCTGGCTG CCCTGCCTCC AGCCATTCAG GAGGAAGTAC TGGCACAGCA GAGAGCTGAG 
CAGCAGCGAC GAGAACTAGC ACAGAATGCC AGCTCAGACA CCCCTATGGA CCCTGTGACC 
TTCATCCAGA CTCTGCCCTC AGACCTGCGC CGTAGTGTCC TAGAGGATAT GGAGGACAGT 
GTGTTAGCTG TGATGCCACC TGACATTGCA GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA 
GAAGCCCGGC AGCGACAGCT CATGCATGAG CGTCTGTTTG GGCACAGTAG CACCTCCGCA 
CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC 
CAGTATACTC GCCTTGCTGT GCAGAGAGGT GGCACCTTCC AGATGGGGGG TAGCAGCAGC 
CATAACAGGC CTTCTGGCAG TAATGTAGAT ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT 
CTGGACCACG AAGCCCTTTC TTGTCTCTTG GTCCTACTTT TTGTGGATGA GCCAAAGCTC 
AATACTAGCC GTCTACACCG AGTACTGAGA AATCTCTGCT ACCATGCCCA GACCCGCCAC 
TGGGTCATCC GCAGTCTGCT CTCCATCTTG CAGCGCAGCA GTGAGAGTGA GCTATGCATT 
GAAACACCCA AACTCACTAC AAGTGAGGAA AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG 
TCAAGTAGCC ATGAGAACCG TCCCCTGGAC CTGCTACACA AGATGGAGTC AAAGAGCTCC 
AACCAGCTTT CCTGGCTCTC AGTATCCATG GATGCAGCCC TAGGCTGCAG GACTAATATA 
TTTCAGATCC AGCGTTCAGG GGGGCGTAAA CATACCGAGA AGCATGCAAG CGGTGGCTCC 
ACCGTCCACA TCCATCCCCA AGCTGCTCCT GTTGTCTGCA GACACGTTTT GGATACACTC 
ATTCAATTGG CCAAGGTATT TCCCAGCCAC TTCACACAGC AGCGGACCAA AGAAACAAAC 
TGTGAGAGTG ATCGGGAAAG GGGCAATAAG GCCTGTAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TTTGCACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTC 
AGCCGGAAAG GCAAGAACTC CGTGAAGTCA GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA 
ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAC 
CCAGTCATCC GCCGGAGCTC TCTCTTAACT GAGAAACTCC TCAGACTCCT TTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAGGTGTCA GAAGCACAGG CTAATTCTGG CAGCGGTGCT 
TCCTCCACCA CCACTGCCAC CTCAACCACA TCTACCACCA CCACCACTGC CGCCTCCACC 
ACGCCCACAC CCCCTACTGC ACCCACCCCT GTCACTTCTG CTCCAGCCCT GGTTGCTGCC 
ACGGCTATTT CCACCATTGT CGTAGCTGCT TCGACCACAG TGACTACCCC CACGACTGCT 
ACCACTACTG TTTCAATTTC TCCCACTACT AAGGGCAGCA AATCTCCAGC GAAGGTGAGT 
GATGGGGGCA GCAGCAGTAC AGACTTTAAG ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAGGAAGG CTTAGAGGAT 
GCAGCCAACG TACTACTGCA GCTCTCCCGG GGGGACTCTG GGACCCGGGA CACTGTTCTC 
AAGCTGCTAC TGAATGGAGC CCGCCATCTG GGTTATACCC TTTGTAAACA AATAGGTACC 
CTGCTGGCCG AGCTGCGGGA ATACAACCTC GAGCAGCAGC GGCGAGCCCA ATGTGAAACC 
CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG CCACAGACCA CCAAGCTGAA GGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAGAATGTG GTAATTGTGG CATCTCAGAA GCGACCTTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTCC GGGACGACAC GCGCCGGGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTAG CCAGTCAGAG 
GCGTCTGTCC GGAGGGAGGA ATCACCCATG GATGTGGACC AGCCATCTCC CAGTGCTCAA 
GATACTCAAT CCATTGCCTC CGATGGAACC CCACAGGGGG AGAAGGAAAA GGAAGAAAGA 
CCACCTGAGT TACCCCTGCT CAGCGAGCAG CTGAGTTTGG ACGAGCTGTG GGACATGCTT 
GGGGAGTGTC TAAAGGAACT AGAGGAATCC CATGACCAGC ATGCGGTGCT AGTGCTACAG 
CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT GCCACAGAGC GGGAGAGCAA GCCTCCTGTC 
CGAGACACCC GTGAGAGCCA GCTGGCACAC ATCAAGGACG AGCCTCCTCC ACTCTCCCCT 
GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC CTTGACCCAT TCTTCTCCCG GGAGCCCTCA 
TCTATGCACA TCTCCTCAAG CCTGCCCCCT GACACACAGA AGTTCCTTCG CTTTGCAGAG 
ACTCACCGCA CTGTGTTAAA CCAGATCCTA CGGCAGTCCA CGACCCACCT TGCTGATGGG 
CCTTTTGCTG TCCTGGTAGA CTACATTCGT GTCCTCGACT TTGATGTCAA GCGCAAATAT 
TTCCGCCAAG AGCTGGAGCG TTTAGATGAG GGGCTCCGGA AAGAAGACAT GGCTGTGCAT 
GTCCGTCGTG ACCATGTGTT TGAAGACTCC TATCGTGAGC TGCATCGCAA ATCCCCCGAA 
GAAATGAAGA ATCGATTGTA TATAGTATTT GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG 
CTCCTGCGGG AGTGGTATAT GATCATCTCT CGAGAGATGT TTAACCCTAT GTATGCCTTG 
TTCCGTACCT CACCTGGTGA TCGAGTCACC TACACCATCA ATCCATCTTC CCACTGCAAC 
CCCAACCACC TCAGCTACTT CAAGTTTGTC GGACGCATTG TGGCCAAAGC TGTATATGAC 
AACCGTCTTC TGGAGTGCTA CTTTACTCGA TCCTTTTACA AACACATCTT GGGCAAGTCA 
GTCAGATATA CAGATATGGA GAGTGAAGAT TACCACTTCT ACCAAGGTCT GGTTTATCTG 
CTGGAAAATG ATGTCTCCAC ACTAGGCTAT GACCTCACCT TCAGCACTGA GGTCCAAGAG 
TTTGGAGTTT GTGAAGTTCG TGACCTCAAA CCCAATGGGG CCAACATCTT GGTAACAGAG 
GAGAATAAGA AGGAGTATGT ACACCTGGTA TGCCAGATGA GAATGACAGG AGCCATCCGC 
AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC TATGAGATCA TTCCAAAGCG CCTCATTTCC 
ATCTTCACTG AGCAGGAGTT AGAGCTGCTT ATATCAGGAC TGCCCACCAT TGACATCGAT 
GATCTGAAAT CCAACACTGA ATACCACAAG TACCAGTCCA ACTCTATTCA GATCCAGTGG 
TTCTGGAGAG CATTGCGTTC TTTCGATCAA GCTGACCGTG CCAAGTTCCT CCAGTTTGTC 
ACAGGTACTT CCAAGGTACC CCTGCAAGGC TTTGCTGCCC TCGAAGGCAT GAATGGCATT 
CAGAAGTTTC AGATCCATCG AGATGACAGG TCCACAGATC GCCTGCCTTC AGCTCACACA 
TGTTTTAATC AGCTGGATCT GCCTGCCTAT GAGAGCTTTG AGAAGCTCCG CCACATGCTA 
CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113584.3
CDS	404..13528
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	NM_031407.6
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031407.6

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAATCAGC AAACAGTATC 
TTGTATAATG GCTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACGGA TAAGCAGCTT 
ATGGAGATTA AAGCAGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT 
CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCA 
GTGCTTGTAA GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATACCCTCAC 
CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC 
GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGATA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT 
GAGCATGAAG TAGATTTGTG CCGAAAAGAA TGTCCGTTTG TGATCAAGCC AAAGATCCAG 
AGACCCAATA CTACACAAGA AGGAGAGGAA ATGGAAACTG ATATGGATGG AGTCCAGTGT 
ATTCCACAAC GAGCAGCACT TCTGAAATCC ATGTTGAATT TCCTCAAGAA GGCCATCCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTCTGCC TACCTCCCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGCCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAACCA TCACTGCTCT CCTCACTCCA GGACAATGGA 
TTGACAGATG TCATGCTGCA TGCACTGCTT ATCAAAGATG TTCCTGCTAC CCGTGAAGTC 
CTTGGCTCCC TCCCAAATGT ATTCAGTGCA CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTTCAGT GTCAGCCTTT TGAACGCCTC TTCAAAGTTC TTCTGTCTCC AGATTACCTC 
CCAGCCATGC GGAGGAGGAG AAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTCG ATGAGCTCAT GAGACATCAG CCCACCCTTA AAACAGATGC AACGACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGGG ACCCCAAATA CATCTGTCAG 
AAGCCATCAA TCCAGAAGGC AGATGGCACT GCCACTGCTC CTCCCCCAAG GTCTAATCAT 
GCCGCAGAAG AAGCCTCTAG TGAGGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTA CCCAGCAAAA TGAAACTGAG CCTAATCAGC AGGTTGTTGG TACAGAGGAA 
CGTATTCCTA TTCCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
CTGAGCAACA ATACAACAGA TGACCACTGC CAGGAATTTG TGAATCAGAA AGGACTGTTG 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACAC TGTCACATGA ACCCAAAGTC 
CTTCAAGAGG GTCTCCTTCA GTTGGACTCC ATCCTCTCCT CCCTGGAGCC CTTACACCGC 
CCCATTGAAT CCCCTGGGGG CTCAGTGTTG TTGCGAGAAC TGGCTTGCGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC CCAGGCCACA CCTCTGCTGC ATGCACTCAC TGCTGCCCAT 
GCCTACATCA TGATGTTTGT TCATACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCCATC 
TCCGTAAACC AGTGGGGCTC TCAATTGGGT CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA 
TACTGTTCCC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TGTGTACCCC AAACAGCCTA 
CCATCTGGGT GTGAATTTGG CCAGGCAGAT ATGCAGAAAC TGGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGGGAAC AGGATGGAGC AGCTGGAAGT 
ATGGATGCTT CTACCCAGGG CTTATTAGAA GGCATTGGGC TAGATGGTGA CACATTGGCT 
CCCATGGAGA CAGATGAACC TACTGCTTCA GACTCTAAGG GCAAATCTAA AATCACACCA 
GCAATGGCTG CCAGAATTAA GCAAATCAAG CCTTTGTTAT CAGCTTCCTC CAGATTAGGC 
CGAGCACTTG CTGAGCTATT TGGACTTCTT GTTAAACTTT GTGTGGGATC TCCTGTCCGC 
CAGAGAAGGA GCCATCATGC TGCCAGCACC ACTACAGCAC CGACACCTGC CGCGCGATCA 
ACAGCCTCAG CTCTCACTAA GCTCTTGACT AAGGGGTTAT CTTGGCAGCC CCCACCATAT 
ACACCTACTC CCCGATTCAG GCTGACATTC TTCATCTGTT CAGTTGGTTT CACATCCCCA 
ATGCTGTTTG ATGAGAGGAA GTATCCCTAC CACCTCATGC TGCAAAAATT TCTCTGCTCC 
GGAGGCCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TGTCCATGGG AGGTAAAGTT 
CCTGTTTCTG AGGGATTGGA ACACTCAGAC TTGCCTGATG GCACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTGGTGGA GAAGATGGTG AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGCTGCCTG CCAAATTGCC TGGAGGTGTC CAGAACTTTC CCCAGTTCAG TGCACTGCGC 
TTCCTTGTGG TAACTCAGAA AGCAGCCTTT ACTTGCATCA AAAACTTATG GAACCGGAAA 
CCCCTGAAGG TATATGGTGG ACGAATGGCT GAATCGATGC TGGCCATTCT ATGCCACATC 
CTCCGAGGAG AACCTGTGAT TCGAGAGAGA CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA 
GAAGAGGATA CAGGGCAAGA GGAAGGTGGC TCCCGCCGGG AACCTCAAGT CAACCAGCAA 
CAACTGCAAC AGCTCATGGA CATGGGCTTC ACAAGGGAAC ATGCAATGGA GGCACTGTTG 
AACACCAGCA CCATGGAGCA GGCCACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATG 
GGAGGAGTTG TTCGGGATCT CAGCATGTCT GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA 
TGTCTAGAGA AGTTCCAGGA TGCTGACCCG TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACTATGT TGCCAGGCTG CTTCCACCTT CTTGATGAGC TGCCAGACAC AGTATACCGT 
GTGTGTGACC TGATCATGAC AGCAATCAAA CGTAATGGAG CAGATTATCG TGACATGATT 
CTGAAGCAAG TAGTCAATCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCCCTGACAA CAAGTGACAC AAAAACCGTG TCAGAGTGGA TAAGTCAGAT GGCCACACTG 
CCCCAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CGCTACTTTT TGAGGAGTTG 
AAGCTACCTT GTGCTTGGGT GGTTGAATCA AGTGGCATCC TTAATGTCCT AATCAAACTC 
TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA GCCAAGGAGC AGAAGGAAGT CCAGACCCCA 
AAGTGGATCA CACCAGTGTT GCTCCTGATT GATTTCTATG AAAAGACAGC CATCTCCTCA 
AAAAGGAGAG CCCAGATGAC TAAGTACCTG CAATCCAACA GCAACAACTG GCGCTGGTTT 
GATGATCGCT CTGGGCGTTG GTGTAGTTAC AGTGCAAGCA ACAATAGCAC TATTGATTCT 
GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA TTCACTGCAG GCCGAAGAAG ATACACGGTC 
CAATTCACTA CAATGGTGCA GGTTAATGAG GAAACAGGGA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTACCTCG GCTGAATAAA AATTCAAAAA ACAGCAATGG ACAGGAACTA 
GAGAAGACGC TGGAAGAAAG CAAAGAAATG GATATCAAAC GTAAAGAAAA TAAAGGCAAT 
GATACCCCTT TGGCCCTAGA GAGTACAAAC ACTGAAAAGG AGACAAGCCT GGAGGAAACA 
AAAATCGGGG AGATCCTGAT CCAGGGCTTG ACAGAAGATA TGGTGACTGT TTTAATCCGG 
GCCTGCGTGA GCATGCTGGG AGTCCCTGTG GACCCAGATA CTTTGCATGC CACCCTTCGT 
CTCTGTCTGA GGCTCACCCG GGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACCCGCATGA TCTTGAATTT GACCCAGAGC TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC 
ACCCTTCTCT TAAGACACAT CATTGAGGAC CCCTGTACCC TTCGTCATAC CATGGAAAAG 
GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT GGTAGCACTA CCTCTGGTGT TGTGTCTGGC 
AGCCTCGGCT CTCGGGAGAT CAACTACATC CTTCGTGTCC TTGGGCCAGC CGCATGCCGC 
AATCCAGACA TATTCACAGA AGTGGCCAAC TGCTGTATCC GCATCGCCCT TCCTGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGCCCTAAT 
GCTGTACAGC TGGTGAAGAC CACCCCTTTG AAGCCCTCAC CTCTGCCTGT CATCCCTGAT 
ACTATCAAGG AAGTGATCTA TGATATGCTG AATGCTCTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGATA AATCTGATCC TAAACCTGGG GTTATGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGATGATGT ATACCAGCAG TACCGGTCAC TTACGCGTCA GAGCAGTGAC 
TTTGATACGC AGTCAGGTTT TTCCATTAAT AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC 
ACTGAGACTT CCGCATCTGG GACCTCCCAA GGAGAGGCTT CAACTCCAGA GGAGTCTCGA 
GATGGGAAGA AAGATAAAGA AGGGGACCGG GCCTCTGAGG AAGGCAAACA GAAAGGCAAG 
GGCAGCAAAC CTTTAATGCC TACCTCCACT ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG 
TCCTATGTTG GTATTGCTAC CCTGATTGCC AACTACAGCT ACACTGTGGG CCAGTCTGAA 
CTGATCAAAG AGGACTGCAG TGTGCTAGCT TTTGTTCTGG ACCACCTGCT CCCACATACC 
CAGAATGCAG AAGACAAGGA CACCCCTGCC TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT 
GCTGCAGGGA GTGGCACAGA TGCCCAGGTG GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT 
GGACGGGCAC TGGCTATGGC TGAGAGTACA GAGAAACATG CCAGGCTTCA GGCAGTGATG 
TGTATCATCA GTACTATCAT GGAGTCCTGC CCCTCCACCT CCAGCTTCTA CAGCAGTGCC 
ACAGCGAAGA CCCAGCACAA TGGCATGAAC AACATCATTC GGCTTTTCCT GAAGAAGGGA 
CTGGTTAATG ACCTGGCCAG AGTACCTCAC AGCTTAGACC TGTCCAGTCC CAACATGGCC 
AACACAGTCA ATGCTGCTCT GAAGCCTTTG GAAACACTTT CCCGGATTGT GAACCAGCCC 
AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT AGCAAGAACA AGTCTGAGCA GGATGCCCAA 
GGAGCCTCTC AAGATTCCAG TAGCAACCAG CAGGACCCAG GCGAGCCTGG GGAAGCAGAA 
GTGCAGGAGG AGGATCATGA TGTCACTCAG ACAGAGGTGG CAGATGGGGA TATCATGGAT 
GGGGAGGCTG AAACCGACTC AGTGGTGATT GCTGGGCAGC CTGAGGTGCT CAGTTCACAA 
GAGATGCAGG TTGAGAATGA GCTGGAGGAC CTGATAGATG AGTTGCTTGA GAGGGATGGC 
GGATCTGGGA ACAGTACAAT TATAGTGAGC AGAAGTGGAG AGGATGAATC ACAAGAGGAC 
GTGCTGATGG ATGAAGCTCC TTCCAACCTC AGCCAAGCTT CCACCTTGCA GGCCAACCGA 
GAAGATTCCA TGAATATCCT GGACCCTGAG GATGAGGAGG AGCACACTCA GGAAGAGGAC 
AGCAGTGGCA GTAACGAGGA TGAGGATGAT AGTCAGGATG AAGAGGAGGA GGAGGAGGAA 
GATGAGGAAG ATGATCAGGA GGATGATGAA GGTGAAGAGG GAGATGAAGA CGATGACGAC 
GATGGCTCTG AGATGGAATT GGATGAGGAT TATCCTGATA TGAACGCTTC TCCCTTGGTC 
CGATTTGAGC GCTTTGACCG GGAGGATGAT CTCATCATTG AGTTTGACAA CATGTTCTCC 
AGTGCTACAG ACATCCCCCC ATCCCCAGGA AATATCCCTA CCACCCATCC ACTGATGGTG 
CGCCATGCAG ACCACAGTTC TCTGACACTG GGCAGTGGCT CTTCAACAAC TCGTCTCACC 
CAGGGCATCG GGCGCAGTCA GAGGACCCTA AGGCAGCTGA CGGCCAATAC TGGCCACACC 
ATTCATGTTC ACTACCCTGG GAATCGCCAG CCCAACCCTC CTCTTATACT GCAGAGGTTG 
CTTGGTCCCT CAGCTGCTGC TGACATCCTT CAGCTGAGCA GCAGCCTTCC CCTACAAAGC 
CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC GATGACGTCC ACATCATCGC CCGTTCTGAT 
GATGAGCTGC TGGATGACTT TTTCCATGAT CAGAGCACAG CTACCAGCCA AGCAGGAACC 
CTGTCCAGCA TCCCCACAGC CCTGACCCGC TGGACAGAAG AATGCAAAGT TCTCGATGCT 
GAGAGCATGC ATGACTGTGT TTCAGTGGTT AAAGTGTCCA TTGTCAATCA CCTGGAATTC 
CTGAGGGATG AGGAGCTGGA AGAAAGGCGA GAGAAGCGCA GGAAACAACT GGCTGAGGAA 
GAAACAAAGA TAACTGATAA AGGCAAAGAA GATAAGGAGA ACAGGGATCA GAGTGCACAG 
TGTACTGCAT CTAAGTCAAA TGACTCCACT GAACAGAATC TCTCAGATGG GACGCCTATG 
CCTGACAGCT ACCCAACAAC CCCATCTTCA ACTGATGCAG CTACATCTGA GTCCAAGGAG 
ACCCTTGGCA CTCTGCAATC CTCACAACAG CAACCAACAC TCCCAACCCC ACCAGCTTTG 
GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA GCTGGAGAAG GGGGCAGCTC TACACAGCTA 
TTGATGCCTG TAGAGCCAGA GGAATTGGGT CCCACAAGGC CAAGTGGGGA AGCAGAAACA 
ACTCAGATGG AGTTATCCCC AGCTCCCACT ATAACCTCAC TTTCCCCAGA GAGAGCTGAG 
GATTCTGATG CACTGACGGC TGTCAGCAGT CAGCTAGAAG GCTCTCCTAT GGATACAAGC 
AGCCTGGCTT CCTGTACCTT AGAGGAGGCT GTGGGTGACA CTTCAGCAGC TGGCAGTTCT 
GAGCAGCCCA GAGCAGGCAG CTCCACTCCT GGGGATGCCC CACCAGCTGT GGCGGAAGTG 
CAAGGCAGGA GTGATGGGTC AGGGGAATCT GCCCAGCCAC CTGAGGACAG CTCCCCACCT 
GCATCCTCTG AGAGCTCTTC CACCAGAGAT TCTGCCGTGG CCATTTCTGG AGCAGATTCC 
CGAGGAATCC TAGAAGAGCC GTTGCCTTCA ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG 
GGTATCAGTC TCCCTGAAGG TGTGGACCCC TCTTTTCTGG CTGCCCTGCC TGATGACATC 
CGTCGGGAAG TTCTACAGAA CCAGCTAGGC ATTCGTCCAC CAACCCGGAC TGCCCCCTCC 
ACAAATAGCT CAGCGCCTGC AGTGGTGGGG AATCCTGGTG TGACTGAAGT GAGCCCTGAG 
TTTCTGGCTG CCCTGCCTCC AGCCATTCAG GAGGAAGTAC TGGCACAGCA GAGAGCTGAG 
CAGCAGCGAC GAGAACTAGC ACAGAATGCC AGCTCAGACA CCCCTATGGA CCCTGTGACC 
TTCATCCAGA CTCTGCCCTC AGACCTGCGC CGTAGTGTCC TAGAGGATAT GGAGGACAGT 
GTGTTAGCTG TGATGCCACC TGACATTGCA GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA 
GAAGCCCGGC AGCGACAGCT CATGCATGAG CGTCTGTTTG GGCACAGTAG CACCTCCGCA 
CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC 
CAGTATACTC GCCTTGCTGT GCAGAGAGGT GGCACCTTCC AGATGGGGGG TAGCAGCAGC 
CATAACAGGC CTTCTGGCAG TAATGTAGAT ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT 
CTGGACCACG AAGCCCTTTC TTGTCTCTTG GTCCTACTTT TTGTGGATGA GCCAAAGCTC 
AATACTAGCC GTCTACACCG AGTACTGAGA AATCTCTGCT ACCATGCCCA GACCCGCCAC 
TGGGTCATCC GCAGTCTGCT CTCCATCTTG CAGCGCAGCA GTGAGAGTGA GCTATGCATT 
GAAACACCCA AACTCACTAC AAGTGAGGAA AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG 
TCAAGTAGCC ATGAGAACCG TCCCCTGGAC CTGCTACACA AGATGGAGTC AAAGAGCTCC 
AACCAGCTTT CCTGGCTCTC AGTATCCATG GATGCAGCCC TAGGCTGCAG GACTAATATA 
TTTCAGATCC AGCGTTCAGG GGGGCGTAAA CATACCGAGA AGCATGCAAG CGGTGGCTCC 
ACCGTCCACA TCCATCCCCA AGCTGCTCCT GTTGTCTGCA GACACGTTTT GGATACACTC 
ATTCAATTGG CCAAGGTATT TCCCAGCCAC TTCACACAGC AGCGGACCAA AGAAACAAAC 
TGTGAGAGTG ATCGGGAAAG GGGCAATAAG GCCTGTAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TTTGCACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTC 
AGCCGGAAAG GCAAGAACTC CGTGAAGTCA GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA 
ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAC 
CCAGTCATCC GCCGGAGCTC TCTCTTAACT GAGAAACTCC TCAGACTCCT TTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAGGTGTCA GAAGCACAGG CTAATTCTGG CAGCGGTGCT 
TCCTCCACCA CCACTGCCAC CTCAACCACA TCTACCACCA CCACCACTGC CGCCTCCACC 
ACGCCCACAC CCCCTACTGC ACCCACCCCT GTCACTTCTG CTCCAGCCCT GGTTGCTGCC 
ACGGCTATTT CCACCATTGT CGTAGCTGCT TCGACCACAG TGACTACCCC CACGACTGCT 
ACCACTACTG TTTCAATTTC TCCCACTACT AAGGGCAGCA AATCTCCAGC GAAGGTGAGT 
GATGGGGGCA GCAGCAGTAC AGACTTTAAG ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAGGAAGG CTTAGAGGAT 
GCAGCCAACG TACTACTGCA GCTCTCCCGG GGGGACTCTG GGACCCGGGA CACTGTTCTC 
AAGCTGCTAC TGAATGGAGC CCGCCATCTG GGTTATACCC TTTGTAAACA AATAGGTACC 
CTGCTGGCCG AGCTGCGGGA ATACAACCTC GAGCAGCAGC GGCGAGCCCA ATGTGAAACC 
CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG CCACAGACCA CCAAGCTGAA GGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAGAATGTG GTAATTGTGG CATCTCAGAA GCGACCTTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTCC GGGACGACAC GCGCCGGGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTAG CCAGTCAGAG 
GCGTCTGTCC GGAGGGAGGA ATCACCCATG GATGTGGACC AGCCATCTCC CAGTGCTCAA 
GATACTCAAT CCATTGCCTC CGATGGAACC CCACAGGGGG AGAAGGAAAA GGAAGAAAGA 
CCACCTGAGT TACCCCTGCT CAGCGAGCAG CTGAGTTTGG ACGAGCTGTG GGACATGCTT 
GGGGAGTGTC TAAAGGAACT AGAGGAATCC CATGACCAGC ATGCGGTGCT AGTGCTACAG 
CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT GCCACAGAGC GGGAGAGCAA GCCTCCTGTC 
CGAGACACCC GTGAGAGCCA GCTGGCACAC ATCAAGGACG AGCCTCCTCC ACTCTCCCCT 
GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC CTTGACCCAT TCTTCTCCCG GGAGCCCTCA 
TCTATGCACA TCTCCTCAAG CCTGCCCCCT GACACACAGA AGTTCCTTCG CTTTGCAGAG 
ACTCACCGCA CTGTGTTAAA CCAGATCCTA CGGCAGTCCA CGACCCACCT TGCTGATGGG 
CCTTTTGCTG TCCTGGTAGA CTACATTCGT GTCCTCGACT TTGATGTCAA GCGCAAATAT 
TTCCGCCAAG AGCTGGAGCG TTTAGATGAG GGGCTCCGGA AAGAAGACAT GGCTGTGCAT 
GTCCGTCGTG ACCATGTGTT TGAAGACTCC TATCGTGAGC TGCATCGCAA ATCCCCCGAA 
GAAATGAAGA ATCGATTGTA TATAGTATTT GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG 
CTCCTGCGGG AGTGGTATAT GATCATCTCT CGAGAGATGT TTAACCCTAT GTATGCCTTG 
TTCCGTACCT CACCTGGTGA TCGAGTCACC TACACCATCA ATCCATCTTC CCACTGCAAC 
CCCAACCACC TCAGCTACTT CAAGTTTGTC GGACGCATTG TGGCCAAAGC TGTATATGAC 
AACCGTCTTC TGGAGTGCTA CTTTACTCGA TCCTTTTACA AACACATCTT GGGCAAGTCA 
GTCAGATATA CAGATATGGA GAGTGAAGAT TACCACTTCT ACCAAGGTCT GGTTTATCTG 
CTGGAAAATG ATGTCTCCAC ACTAGGCTAT GACCTCACCT TCAGCACTGA GGTCCAAGAG 
TTTGGAGTTT GTGAAGTTCG TGACCTCAAA CCCAATGGGG CCAACATCTT GGTAACAGAG 
GAGAATAAGA AGGAGTATGT ACACCTGGTA TGCCAGATGA GAATGACAGG AGCCATCCGC 
AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC TATGAGATCA TTCCAAAGCG CCTCATTTCC 
ATCTTCACTG AGCAGGAGTT AGAGCTGCTT ATATCAGGAC TGCCCACCAT TGACATCGAT 
GATCTGAAAT CCAACACTGA ATACCACAAG TACCAGTCCA ACTCTATTCA GATCCAGTGG 
TTCTGGAGAG CATTGCGTTC TTTCGATCAA GCTGACCGTG CCAAGTTCCT CCAGTTTGTC 
ACAGGTACTT CCAAGGTACC CCTGCAAGGC TTTGCTGCCC TCGAAGGCAT GAATGGCATT 
CAGAAGTTTC AGATCCATCG AGATGACAGG TCCACAGATC GCCTGCCTTC AGCTCACACA 
TGTTTTAATC AGCTGGATCT GCCTGCCTAT GAGAGCTTTG AGAAGCTCCG CCACATGCTA 
CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( NM_031407.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_113584.3 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), mRNA.

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CloneID	OHu23451
Clone ID Related Accession (Same CDS sequence)	XM_005261965.3 , XM_005261965.4 , NM_031407.6 , NM_031407.7
Accession Version	NM_031407.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ097177.1, BX323845.7, AY772009.1, AC231658.3, AB002310.3 and R60532.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_031407.6. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ097177.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262854.11/ ENSP00000262854.6 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAATCAGC AAACAGTATC 
TTGTATAATG GCTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACGGA TAAGCAGCTT 
ATGGAGATTA AAGCAGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT 
CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCA 
GTGCTTGTAA GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATACCCTCAC 
CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC 
GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGATA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT 
GAGCATGAAG TAGATTTGTG CCGAAAAGAA TGTCCGTTTG TGATCAAGCC AAAGATCCAG 
AGACCCAATA CTACACAAGA AGGAGAGGAA ATGGAAACTG ATATGGATGG AGTCCAGTGT 
ATTCCACAAC GAGCAGCACT TCTGAAATCC ATGTTGAATT TCCTCAAGAA GGCCATCCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTCTGCC TACCTCCCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGCCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAACCA TCACTGCTCT CCTCACTCCA GGACAATGGA 
TTGACAGATG TCATGCTGCA TGCACTGCTT ATCAAAGATG TTCCTGCTAC CCGTGAAGTC 
CTTGGCTCCC TCCCAAATGT ATTCAGTGCA CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTTCAGT GTCAGCCTTT TGAACGCCTC TTCAAAGTTC TTCTGTCTCC AGATTACCTC 
CCAGCCATGC GGAGGAGGAG AAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTCG ATGAGCTCAT GAGACATCAG CCCACCCTTA AAACAGATGC AACGACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGGG ACCCCAAATA CATCTGTCAG 
AAGCCATCAA TCCAGAAGGC AGATGGCACT GCCACTGCTC CTCCCCCAAG GTCTAATCAT 
GCCGCAGAAG AAGCCTCTAG TGAGGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTA CCCAGCAAAA TGAAACTGAG CCTAATCAGC AGGTTGTTGG TACAGAGGAA 
CGTATTCCTA TTCCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
CTGAGCAACA ATACAACAGA TGACCACTGC CAGGAATTTG TGAATCAGAA AGGACTGTTG 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACAC TGTCACATGA ACCCAAAGTC 
CTTCAAGAGG GTCTCCTTCA GTTGGACTCC ATCCTCTCCT CCCTGGAGCC CTTACACCGC 
CCCATTGAAT CCCCTGGGGG CTCAGTGTTG TTGCGAGAAC TGGCTTGCGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC CCAGGCCACA CCTCTGCTGC ATGCACTCAC TGCTGCCCAT 
GCCTACATCA TGATGTTTGT TCATACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCCATC 
TCCGTAAACC AGTGGGGCTC TCAATTGGGT CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA 
TACTGTTCCC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TGTGTACCCC AAACAGCCTA 
CCATCTGGGT GTGAATTTGG CCAGGCAGAT ATGCAGAAAC TGGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGGGAAC AGGATGGAGC AGCTGGAAGT 
ATGGATGCTT CTACCCAGGG CTTATTAGAA GGCATTGGGC TAGATGGTGA CACATTGGCT 
CCCATGGAGA CAGATGAACC TACTGCTTCA GACTCTAAGG GCAAATCTAA AATCACACCA 
GCAATGGCTG CCAGAATTAA GCAAATCAAG CCTTTGTTAT CAGCTTCCTC CAGATTAGGC 
CGAGCACTTG CTGAGCTATT TGGACTTCTT GTTAAACTTT GTGTGGGATC TCCTGTCCGC 
CAGAGAAGGA GCCATCATGC TGCCAGCACC ACTACAGCAC CGACACCTGC CGCGCGATCA 
ACAGCCTCAG CTCTCACTAA GCTCTTGACT AAGGGGTTAT CTTGGCAGCC CCCACCATAT 
ACACCTACTC CCCGATTCAG GCTGACATTC TTCATCTGTT CAGTTGGTTT CACATCCCCA 
ATGCTGTTTG ATGAGAGGAA GTATCCCTAC CACCTCATGC TGCAAAAATT TCTCTGCTCC 
GGAGGCCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TGTCCATGGG AGGTAAAGTT 
CCTGTTTCTG AGGGATTGGA ACACTCAGAC TTGCCTGATG GCACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTGGTGGA GAAGATGGTG AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGCTGCCTG CCAAATTGCC TGGAGGTGTC CAGAACTTTC CCCAGTTCAG TGCACTGCGC 
TTCCTTGTGG TAACTCAGAA AGCAGCCTTT ACTTGCATCA AAAACTTATG GAACCGGAAA 
CCCCTGAAGG TATATGGTGG ACGAATGGCT GAATCGATGC TGGCCATTCT ATGCCACATC 
CTCCGAGGAG AACCTGTGAT TCGAGAGAGA CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA 
GAAGAGGATA CAGGGCAAGA GGAAGGTGGC TCCCGCCGGG AACCTCAAGT CAACCAGCAA 
CAACTGCAAC AGCTCATGGA CATGGGCTTC ACAAGGGAAC ATGCAATGGA GGCACTGTTG 
AACACCAGCA CCATGGAGCA GGCCACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATG 
GGAGGAGTTG TTCGGGATCT CAGCATGTCT GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA 
TGTCTAGAGA AGTTCCAGGA TGCTGACCCG TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACTATGT TGCCAGGCTG CTTCCACCTT CTTGATGAGC TGCCAGACAC AGTATACCGT 
GTGTGTGACC TGATCATGAC AGCAATCAAA CGTAATGGAG CAGATTATCG TGACATGATT 
CTGAAGCAAG TAGTCAATCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCCCTGACAA CAAGTGACAC AAAAACCGTG TCAGAGTGGA TAAGTCAGAT GGCCACACTG 
CCCCAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CGCTACTTTT TGAGGAGTTG 
AAGCTACCTT GTGCTTGGGT GGTTGAATCA AGTGGCATCC TTAATGTCCT AATCAAACTC 
TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA GCCAAGGAGC AGAAGGAAGT CCAGACCCCA 
AAGTGGATCA CACCAGTGTT GCTCCTGATT GATTTCTATG AAAAGACAGC CATCTCCTCA 
AAAAGGAGAG CCCAGATGAC TAAGTACCTG CAATCCAACA GCAACAACTG GCGCTGGTTT 
GATGATCGCT CTGGGCGTTG GTGTAGTTAC AGTGCAAGCA ACAATAGCAC TATTGATTCT 
GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA TTCACTGCAG GCCGAAGAAG ATACACGGTC 
CAATTCACTA CAATGGTGCA GGTTAATGAG GAAACAGGGA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTACCTCG GCTGAATAAA AATTCAAAAA ACAGCAATGG ACAGGAACTA 
GAGAAGACGC TGGAAGAAAG CAAAGAAATG GATATCAAAC GTAAAGAAAA TAAAGGCAAT 
GATACCCCTT TGGCCCTAGA GAGTACAAAC ACTGAAAAGG AGACAAGCCT GGAGGAAACA 
AAAATCGGGG AGATCCTGAT CCAGGGCTTG ACAGAAGATA TGGTGACTGT TTTAATCCGG 
GCCTGCGTGA GCATGCTGGG AGTCCCTGTG GACCCAGATA CTTTGCATGC CACCCTTCGT 
CTCTGTCTGA GGCTCACCCG GGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACCCGCATGA TCTTGAATTT GACCCAGAGC TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC 
ACCCTTCTCT TAAGACACAT CATTGAGGAC CCCTGTACCC TTCGTCATAC CATGGAAAAG 
GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT GGTAGCACTA CCTCTGGTGT TGTGTCTGGC 
AGCCTCGGCT CTCGGGAGAT CAACTACATC CTTCGTGTCC TTGGGCCAGC CGCATGCCGC 
AATCCAGACA TATTCACAGA AGTGGCCAAC TGCTGTATCC GCATCGCCCT TCCTGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGCCCTAAT 
GCTGTACAGC TGGTGAAGAC CACCCCTTTG AAGCCCTCAC CTCTGCCTGT CATCCCTGAT 
ACTATCAAGG AAGTGATCTA TGATATGCTG AATGCTCTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGATA AATCTGATCC TAAACCTGGG GTTATGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGATGATGT ATACCAGCAG TACCGGTCAC TTACGCGTCA GAGCAGTGAC 
TTTGATACGC AGTCAGGTTT TTCCATTAAT AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC 
ACTGAGACTT CCGCATCTGG GACCTCCCAA GGAGAGGCTT CAACTCCAGA GGAGTCTCGA 
GATGGGAAGA AAGATAAAGA AGGGGACCGG GCCTCTGAGG AAGGCAAACA GAAAGGCAAG 
GGCAGCAAAC CTTTAATGCC TACCTCCACT ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG 
TCCTATGTTG GTATTGCTAC CCTGATTGCC AACTACAGCT ACACTGTGGG CCAGTCTGAA 
CTGATCAAAG AGGACTGCAG TGTGCTAGCT TTTGTTCTGG ACCACCTGCT CCCACATACC 
CAGAATGCAG AAGACAAGGA CACCCCTGCC TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT 
GCTGCAGGGA GTGGCACAGA TGCCCAGGTG GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT 
GGACGGGCAC TGGCTATGGC TGAGAGTACA GAGAAACATG CCAGGCTTCA GGCAGTGATG 
TGTATCATCA GTACTATCAT GGAGTCCTGC CCCTCCACCT CCAGCTTCTA CAGCAGTGCC 
ACAGCGAAGA CCCAGCACAA TGGCATGAAC AACATCATTC GGCTTTTCCT GAAGAAGGGA 
CTGGTTAATG ACCTGGCCAG AGTACCTCAC AGCTTAGACC TGTCCAGTCC CAACATGGCC 
AACACAGTCA ATGCTGCTCT GAAGCCTTTG GAAACACTTT CCCGGATTGT GAACCAGCCC 
AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT AGCAAGAACA AGTCTGAGCA GGATGCCCAA 
GGAGCCTCTC AAGATTCCAG TAGCAACCAG CAGGACCCAG GCGAGCCTGG GGAAGCAGAA 
GTGCAGGAGG AGGATCATGA TGTCACTCAG ACAGAGGTGG CAGATGGGGA TATCATGGAT 
GGGGAGGCTG AAACCGACTC AGTGGTGATT GCTGGGCAGC CTGAGGTGCT CAGTTCACAA 
GAGATGCAGG TTGAGAATGA GCTGGAGGAC CTGATAGATG AGTTGCTTGA GAGGGATGGC 
GGATCTGGGA ACAGTACAAT TATAGTGAGC AGAAGTGGAG AGGATGAATC ACAAGAGGAC 
GTGCTGATGG ATGAAGCTCC TTCCAACCTC AGCCAAGCTT CCACCTTGCA GGCCAACCGA 
GAAGATTCCA TGAATATCCT GGACCCTGAG GATGAGGAGG AGCACACTCA GGAAGAGGAC 
AGCAGTGGCA GTAACGAGGA TGAGGATGAT AGTCAGGATG AAGAGGAGGA GGAGGAGGAA 
GATGAGGAAG ATGATCAGGA GGATGATGAA GGTGAAGAGG GAGATGAAGA CGATGACGAC 
GATGGCTCTG AGATGGAATT GGATGAGGAT TATCCTGATA TGAACGCTTC TCCCTTGGTC 
CGATTTGAGC GCTTTGACCG GGAGGATGAT CTCATCATTG AGTTTGACAA CATGTTCTCC 
AGTGCTACAG ACATCCCCCC ATCCCCAGGA AATATCCCTA CCACCCATCC ACTGATGGTG 
CGCCATGCAG ACCACAGTTC TCTGACACTG GGCAGTGGCT CTTCAACAAC TCGTCTCACC 
CAGGGCATCG GGCGCAGTCA GAGGACCCTA AGGCAGCTGA CGGCCAATAC TGGCCACACC 
ATTCATGTTC ACTACCCTGG GAATCGCCAG CCCAACCCTC CTCTTATACT GCAGAGGTTG 
CTTGGTCCCT CAGCTGCTGC TGACATCCTT CAGCTGAGCA GCAGCCTTCC CCTACAAAGC 
CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC GATGACGTCC ACATCATCGC CCGTTCTGAT 
GATGAGCTGC TGGATGACTT TTTCCATGAT CAGAGCACAG CTACCAGCCA AGCAGGAACC 
CTGTCCAGCA TCCCCACAGC CCTGACCCGC TGGACAGAAG AATGCAAAGT TCTCGATGCT 
GAGAGCATGC ATGACTGTGT TTCAGTGGTT AAAGTGTCCA TTGTCAATCA CCTGGAATTC 
CTGAGGGATG AGGAGCTGGA AGAAAGGCGA GAGAAGCGCA GGAAACAACT GGCTGAGGAA 
GAAACAAAGA TAACTGATAA AGGCAAAGAA GATAAGGAGA ACAGGGATCA GAGTGCACAG 
TGTACTGCAT CTAAGTCAAA TGACTCCACT GAACAGAATC TCTCAGATGG GACGCCTATG 
CCTGACAGCT ACCCAACAAC CCCATCTTCA ACTGATGCAG CTACATCTGA GTCCAAGGAG 
ACCCTTGGCA CTCTGCAATC CTCACAACAG CAACCAACAC TCCCAACCCC ACCAGCTTTG 
GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA GCTGGAGAAG GGGGCAGCTC TACACAGCTA 
TTGATGCCTG TAGAGCCAGA GGAATTGGGT CCCACAAGGC CAAGTGGGGA AGCAGAAACA 
ACTCAGATGG AGTTATCCCC AGCTCCCACT ATAACCTCAC TTTCCCCAGA GAGAGCTGAG 
GATTCTGATG CACTGACGGC TGTCAGCAGT CAGCTAGAAG GCTCTCCTAT GGATACAAGC 
AGCCTGGCTT CCTGTACCTT AGAGGAGGCT GTGGGTGACA CTTCAGCAGC TGGCAGTTCT 
GAGCAGCCCA GAGCAGGCAG CTCCACTCCT GGGGATGCCC CACCAGCTGT GGCGGAAGTG 
CAAGGCAGGA GTGATGGGTC AGGGGAATCT GCCCAGCCAC CTGAGGACAG CTCCCCACCT 
GCATCCTCTG AGAGCTCTTC CACCAGAGAT TCTGCCGTGG CCATTTCTGG AGCAGATTCC 
CGAGGAATCC TAGAAGAGCC GTTGCCTTCA ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG 
GGTATCAGTC TCCCTGAAGG TGTGGACCCC TCTTTTCTGG CTGCCCTGCC TGATGACATC 
CGTCGGGAAG TTCTACAGAA CCAGCTAGGC ATTCGTCCAC CAACCCGGAC TGCCCCCTCC 
ACAAATAGCT CAGCGCCTGC AGTGGTGGGG AATCCTGGTG TGACTGAAGT GAGCCCTGAG 
TTTCTGGCTG CCCTGCCTCC AGCCATTCAG GAGGAAGTAC TGGCACAGCA GAGAGCTGAG 
CAGCAGCGAC GAGAACTAGC ACAGAATGCC AGCTCAGACA CCCCTATGGA CCCTGTGACC 
TTCATCCAGA CTCTGCCCTC AGACCTGCGC CGTAGTGTCC TAGAGGATAT GGAGGACAGT 
GTGTTAGCTG TGATGCCACC TGACATTGCA GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA 
GAAGCCCGGC AGCGACAGCT CATGCATGAG CGTCTGTTTG GGCACAGTAG CACCTCCGCA 
CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC 
CAGTATACTC GCCTTGCTGT GCAGAGAGGT GGCACCTTCC AGATGGGGGG TAGCAGCAGC 
CATAACAGGC CTTCTGGCAG TAATGTAGAT ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT 
CTGGACCACG AAGCCCTTTC TTGTCTCTTG GTCCTACTTT TTGTGGATGA GCCAAAGCTC 
AATACTAGCC GTCTACACCG AGTACTGAGA AATCTCTGCT ACCATGCCCA GACCCGCCAC 
TGGGTCATCC GCAGTCTGCT CTCCATCTTG CAGCGCAGCA GTGAGAGTGA GCTATGCATT 
GAAACACCCA AACTCACTAC AAGTGAGGAA AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG 
TCAAGTAGCC ATGAGAACCG TCCCCTGGAC CTGCTACACA AGATGGAGTC AAAGAGCTCC 
AACCAGCTTT CCTGGCTCTC AGTATCCATG GATGCAGCCC TAGGCTGCAG GACTAATATA 
TTTCAGATCC AGCGTTCAGG GGGGCGTAAA CATACCGAGA AGCATGCAAG CGGTGGCTCC 
ACCGTCCACA TCCATCCCCA AGCTGCTCCT GTTGTCTGCA GACACGTTTT GGATACACTC 
ATTCAATTGG CCAAGGTATT TCCCAGCCAC TTCACACAGC AGCGGACCAA AGAAACAAAC 
TGTGAGAGTG ATCGGGAAAG GGGCAATAAG GCCTGTAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TTTGCACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTC 
AGCCGGAAAG GCAAGAACTC CGTGAAGTCA GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA 
ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAC 
CCAGTCATCC GCCGGAGCTC TCTCTTAACT GAGAAACTCC TCAGACTCCT TTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAGGTGTCA GAAGCACAGG CTAATTCTGG CAGCGGTGCT 
TCCTCCACCA CCACTGCCAC CTCAACCACA TCTACCACCA CCACCACTGC CGCCTCCACC 
ACGCCCACAC CCCCTACTGC ACCCACCCCT GTCACTTCTG CTCCAGCCCT GGTTGCTGCC 
ACGGCTATTT CCACCATTGT CGTAGCTGCT TCGACCACAG TGACTACCCC CACGACTGCT 
ACCACTACTG TTTCAATTTC TCCCACTACT AAGGGCAGCA AATCTCCAGC GAAGGTGAGT 
GATGGGGGCA GCAGCAGTAC AGACTTTAAG ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAGGAAGG CTTAGAGGAT 
GCAGCCAACG TACTACTGCA GCTCTCCCGG GGGGACTCTG GGACCCGGGA CACTGTTCTC 
AAGCTGCTAC TGAATGGAGC CCGCCATCTG GGTTATACCC TTTGTAAACA AATAGGTACC 
CTGCTGGCCG AGCTGCGGGA ATACAACCTC GAGCAGCAGC GGCGAGCCCA ATGTGAAACC 
CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG CCACAGACCA CCAAGCTGAA GGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAGAATGTG GTAATTGTGG CATCTCAGAA GCGACCTTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTCC GGGACGACAC GCGCCGGGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTAG CCAGTCAGAG 
GCGTCTGTCC GGAGGGAGGA ATCACCCATG GATGTGGACC AGCCATCTCC CAGTGCTCAA 
GATACTCAAT CCATTGCCTC CGATGGAACC CCACAGGGGG AGAAGGAAAA GGAAGAAAGA 
CCACCTGAGT TACCCCTGCT CAGCGAGCAG CTGAGTTTGG ACGAGCTGTG GGACATGCTT 
GGGGAGTGTC TAAAGGAACT AGAGGAATCC CATGACCAGC ATGCGGTGCT AGTGCTACAG 
CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT GCCACAGAGC GGGAGAGCAA GCCTCCTGTC 
CGAGACACCC GTGAGAGCCA GCTGGCACAC ATCAAGGACG AGCCTCCTCC ACTCTCCCCT 
GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC CTTGACCCAT TCTTCTCCCG GGAGCCCTCA 
TCTATGCACA TCTCCTCAAG CCTGCCCCCT GACACACAGA AGTTCCTTCG CTTTGCAGAG 
ACTCACCGCA CTGTGTTAAA CCAGATCCTA CGGCAGTCCA CGACCCACCT TGCTGATGGG 
CCTTTTGCTG TCCTGGTAGA CTACATTCGT GTCCTCGACT TTGATGTCAA GCGCAAATAT 
TTCCGCCAAG AGCTGGAGCG TTTAGATGAG GGGCTCCGGA AAGAAGACAT GGCTGTGCAT 
GTCCGTCGTG ACCATGTGTT TGAAGACTCC TATCGTGAGC TGCATCGCAA ATCCCCCGAA 
GAAATGAAGA ATCGATTGTA TATAGTATTT GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG 
CTCCTGCGGG AGTGGTATAT GATCATCTCT CGAGAGATGT TTAACCCTAT GTATGCCTTG 
TTCCGTACCT CACCTGGTGA TCGAGTCACC TACACCATCA ATCCATCTTC CCACTGCAAC 
CCCAACCACC TCAGCTACTT CAAGTTTGTC GGACGCATTG TGGCCAAAGC TGTATATGAC 
AACCGTCTTC TGGAGTGCTA CTTTACTCGA TCCTTTTACA AACACATCTT GGGCAAGTCA 
GTCAGATATA CAGATATGGA GAGTGAAGAT TACCACTTCT ACCAAGGTCT GGTTTATCTG 
CTGGAAAATG ATGTCTCCAC ACTAGGCTAT GACCTCACCT TCAGCACTGA GGTCCAAGAG 
TTTGGAGTTT GTGAAGTTCG TGACCTCAAA CCCAATGGGG CCAACATCTT GGTAACAGAG 
GAGAATAAGA AGGAGTATGT ACACCTGGTA TGCCAGATGA GAATGACAGG AGCCATCCGC 
AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC TATGAGATCA TTCCAAAGCG CCTCATTTCC 
ATCTTCACTG AGCAGGAGTT AGAGCTGCTT ATATCAGGAC TGCCCACCAT TGACATCGAT 
GATCTGAAAT CCAACACTGA ATACCACAAG TACCAGTCCA ACTCTATTCA GATCCAGTGG 
TTCTGGAGAG CATTGCGTTC TTTCGATCAA GCTGACCGTG CCAAGTTCCT CCAGTTTGTC 
ACAGGTACTT CCAAGGTACC CCTGCAAGGC TTTGCTGCCC TCGAAGGCAT GAATGGCATT 
CAGAAGTTTC AGATCCATCG AGATGACAGG TCCACAGATC GCCTGCCTTC AGCTCACACA 
TGTTTTAATC AGCTGGATCT GCCTGCCTAT GAGAGCTTTG AGAAGCTCCG CCACATGCTA 
CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113584.3
CDS	394..13518
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	NM_031407.7
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031407.7

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAATCAGC AAACAGTATC 
TTGTATAATG GCTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACGGA TAAGCAGCTT 
ATGGAGATTA AAGCAGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT 
CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCA 
GTGCTTGTAA GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATACCCTCAC 
CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC 
GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGATA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT 
GAGCATGAAG TAGATTTGTG CCGAAAAGAA TGTCCGTTTG TGATCAAGCC AAAGATCCAG 
AGACCCAATA CTACACAAGA AGGAGAGGAA ATGGAAACTG ATATGGATGG AGTCCAGTGT 
ATTCCACAAC GAGCAGCACT TCTGAAATCC ATGTTGAATT TCCTCAAGAA GGCCATCCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTCTGCC TACCTCCCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGCCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAACCA TCACTGCTCT CCTCACTCCA GGACAATGGA 
TTGACAGATG TCATGCTGCA TGCACTGCTT ATCAAAGATG TTCCTGCTAC CCGTGAAGTC 
CTTGGCTCCC TCCCAAATGT ATTCAGTGCA CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTTCAGT GTCAGCCTTT TGAACGCCTC TTCAAAGTTC TTCTGTCTCC AGATTACCTC 
CCAGCCATGC GGAGGAGGAG AAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTCG ATGAGCTCAT GAGACATCAG CCCACCCTTA AAACAGATGC AACGACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGGG ACCCCAAATA CATCTGTCAG 
AAGCCATCAA TCCAGAAGGC AGATGGCACT GCCACTGCTC CTCCCCCAAG GTCTAATCAT 
GCCGCAGAAG AAGCCTCTAG TGAGGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTA CCCAGCAAAA TGAAACTGAG CCTAATCAGC AGGTTGTTGG TACAGAGGAA 
CGTATTCCTA TTCCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
CTGAGCAACA ATACAACAGA TGACCACTGC CAGGAATTTG TGAATCAGAA AGGACTGTTG 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACAC TGTCACATGA ACCCAAAGTC 
CTTCAAGAGG GTCTCCTTCA GTTGGACTCC ATCCTCTCCT CCCTGGAGCC CTTACACCGC 
CCCATTGAAT CCCCTGGGGG CTCAGTGTTG TTGCGAGAAC TGGCTTGCGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC CCAGGCCACA CCTCTGCTGC ATGCACTCAC TGCTGCCCAT 
GCCTACATCA TGATGTTTGT TCATACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCCATC 
TCCGTAAACC AGTGGGGCTC TCAATTGGGT CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA 
TACTGTTCCC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TGTGTACCCC AAACAGCCTA 
CCATCTGGGT GTGAATTTGG CCAGGCAGAT ATGCAGAAAC TGGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGGGAAC AGGATGGAGC AGCTGGAAGT 
ATGGATGCTT CTACCCAGGG CTTATTAGAA GGCATTGGGC TAGATGGTGA CACATTGGCT 
CCCATGGAGA CAGATGAACC TACTGCTTCA GACTCTAAGG GCAAATCTAA AATCACACCA 
GCAATGGCTG CCAGAATTAA GCAAATCAAG CCTTTGTTAT CAGCTTCCTC CAGATTAGGC 
CGAGCACTTG CTGAGCTATT TGGACTTCTT GTTAAACTTT GTGTGGGATC TCCTGTCCGC 
CAGAGAAGGA GCCATCATGC TGCCAGCACC ACTACAGCAC CGACACCTGC CGCGCGATCA 
ACAGCCTCAG CTCTCACTAA GCTCTTGACT AAGGGGTTAT CTTGGCAGCC CCCACCATAT 
ACACCTACTC CCCGATTCAG GCTGACATTC TTCATCTGTT CAGTTGGTTT CACATCCCCA 
ATGCTGTTTG ATGAGAGGAA GTATCCCTAC CACCTCATGC TGCAAAAATT TCTCTGCTCC 
GGAGGCCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TGTCCATGGG AGGTAAAGTT 
CCTGTTTCTG AGGGATTGGA ACACTCAGAC TTGCCTGATG GCACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTGGTGGA GAAGATGGTG AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGCTGCCTG CCAAATTGCC TGGAGGTGTC CAGAACTTTC CCCAGTTCAG TGCACTGCGC 
TTCCTTGTGG TAACTCAGAA AGCAGCCTTT ACTTGCATCA AAAACTTATG GAACCGGAAA 
CCCCTGAAGG TATATGGTGG ACGAATGGCT GAATCGATGC TGGCCATTCT ATGCCACATC 
CTCCGAGGAG AACCTGTGAT TCGAGAGAGA CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA 
GAAGAGGATA CAGGGCAAGA GGAAGGTGGC TCCCGCCGGG AACCTCAAGT CAACCAGCAA 
CAACTGCAAC AGCTCATGGA CATGGGCTTC ACAAGGGAAC ATGCAATGGA GGCACTGTTG 
AACACCAGCA CCATGGAGCA GGCCACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATG 
GGAGGAGTTG TTCGGGATCT CAGCATGTCT GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA 
TGTCTAGAGA AGTTCCAGGA TGCTGACCCG TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACTATGT TGCCAGGCTG CTTCCACCTT CTTGATGAGC TGCCAGACAC AGTATACCGT 
GTGTGTGACC TGATCATGAC AGCAATCAAA CGTAATGGAG CAGATTATCG TGACATGATT 
CTGAAGCAAG TAGTCAATCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCCCTGACAA CAAGTGACAC AAAAACCGTG TCAGAGTGGA TAAGTCAGAT GGCCACACTG 
CCCCAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CGCTACTTTT TGAGGAGTTG 
AAGCTACCTT GTGCTTGGGT GGTTGAATCA AGTGGCATCC TTAATGTCCT AATCAAACTC 
TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA GCCAAGGAGC AGAAGGAAGT CCAGACCCCA 
AAGTGGATCA CACCAGTGTT GCTCCTGATT GATTTCTATG AAAAGACAGC CATCTCCTCA 
AAAAGGAGAG CCCAGATGAC TAAGTACCTG CAATCCAACA GCAACAACTG GCGCTGGTTT 
GATGATCGCT CTGGGCGTTG GTGTAGTTAC AGTGCAAGCA ACAATAGCAC TATTGATTCT 
GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA TTCACTGCAG GCCGAAGAAG ATACACGGTC 
CAATTCACTA CAATGGTGCA GGTTAATGAG GAAACAGGGA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTACCTCG GCTGAATAAA AATTCAAAAA ACAGCAATGG ACAGGAACTA 
GAGAAGACGC TGGAAGAAAG CAAAGAAATG GATATCAAAC GTAAAGAAAA TAAAGGCAAT 
GATACCCCTT TGGCCCTAGA GAGTACAAAC ACTGAAAAGG AGACAAGCCT GGAGGAAACA 
AAAATCGGGG AGATCCTGAT CCAGGGCTTG ACAGAAGATA TGGTGACTGT TTTAATCCGG 
GCCTGCGTGA GCATGCTGGG AGTCCCTGTG GACCCAGATA CTTTGCATGC CACCCTTCGT 
CTCTGTCTGA GGCTCACCCG GGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACCCGCATGA TCTTGAATTT GACCCAGAGC TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC 
ACCCTTCTCT TAAGACACAT CATTGAGGAC CCCTGTACCC TTCGTCATAC CATGGAAAAG 
GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT GGTAGCACTA CCTCTGGTGT TGTGTCTGGC 
AGCCTCGGCT CTCGGGAGAT CAACTACATC CTTCGTGTCC TTGGGCCAGC CGCATGCCGC 
AATCCAGACA TATTCACAGA AGTGGCCAAC TGCTGTATCC GCATCGCCCT TCCTGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGCCCTAAT 
GCTGTACAGC TGGTGAAGAC CACCCCTTTG AAGCCCTCAC CTCTGCCTGT CATCCCTGAT 
ACTATCAAGG AAGTGATCTA TGATATGCTG AATGCTCTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGATA AATCTGATCC TAAACCTGGG GTTATGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGATGATGT ATACCAGCAG TACCGGTCAC TTACGCGTCA GAGCAGTGAC 
TTTGATACGC AGTCAGGTTT TTCCATTAAT AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC 
ACTGAGACTT CCGCATCTGG GACCTCCCAA GGAGAGGCTT CAACTCCAGA GGAGTCTCGA 
GATGGGAAGA AAGATAAAGA AGGGGACCGG GCCTCTGAGG AAGGCAAACA GAAAGGCAAG 
GGCAGCAAAC CTTTAATGCC TACCTCCACT ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG 
TCCTATGTTG GTATTGCTAC CCTGATTGCC AACTACAGCT ACACTGTGGG CCAGTCTGAA 
CTGATCAAAG AGGACTGCAG TGTGCTAGCT TTTGTTCTGG ACCACCTGCT CCCACATACC 
CAGAATGCAG AAGACAAGGA CACCCCTGCC TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT 
GCTGCAGGGA GTGGCACAGA TGCCCAGGTG GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT 
GGACGGGCAC TGGCTATGGC TGAGAGTACA GAGAAACATG CCAGGCTTCA GGCAGTGATG 
TGTATCATCA GTACTATCAT GGAGTCCTGC CCCTCCACCT CCAGCTTCTA CAGCAGTGCC 
ACAGCGAAGA CCCAGCACAA TGGCATGAAC AACATCATTC GGCTTTTCCT GAAGAAGGGA 
CTGGTTAATG ACCTGGCCAG AGTACCTCAC AGCTTAGACC TGTCCAGTCC CAACATGGCC 
AACACAGTCA ATGCTGCTCT GAAGCCTTTG GAAACACTTT CCCGGATTGT GAACCAGCCC 
AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT AGCAAGAACA AGTCTGAGCA GGATGCCCAA 
GGAGCCTCTC AAGATTCCAG TAGCAACCAG CAGGACCCAG GCGAGCCTGG GGAAGCAGAA 
GTGCAGGAGG AGGATCATGA TGTCACTCAG ACAGAGGTGG CAGATGGGGA TATCATGGAT 
GGGGAGGCTG AAACCGACTC AGTGGTGATT GCTGGGCAGC CTGAGGTGCT CAGTTCACAA 
GAGATGCAGG TTGAGAATGA GCTGGAGGAC CTGATAGATG AGTTGCTTGA GAGGGATGGC 
GGATCTGGGA ACAGTACAAT TATAGTGAGC AGAAGTGGAG AGGATGAATC ACAAGAGGAC 
GTGCTGATGG ATGAAGCTCC TTCCAACCTC AGCCAAGCTT CCACCTTGCA GGCCAACCGA 
GAAGATTCCA TGAATATCCT GGACCCTGAG GATGAGGAGG AGCACACTCA GGAAGAGGAC 
AGCAGTGGCA GTAACGAGGA TGAGGATGAT AGTCAGGATG AAGAGGAGGA GGAGGAGGAA 
GATGAGGAAG ATGATCAGGA GGATGATGAA GGTGAAGAGG GAGATGAAGA CGATGACGAC 
GATGGCTCTG AGATGGAATT GGATGAGGAT TATCCTGATA TGAACGCTTC TCCCTTGGTC 
CGATTTGAGC GCTTTGACCG GGAGGATGAT CTCATCATTG AGTTTGACAA CATGTTCTCC 
AGTGCTACAG ACATCCCCCC ATCCCCAGGA AATATCCCTA CCACCCATCC ACTGATGGTG 
CGCCATGCAG ACCACAGTTC TCTGACACTG GGCAGTGGCT CTTCAACAAC TCGTCTCACC 
CAGGGCATCG GGCGCAGTCA GAGGACCCTA AGGCAGCTGA CGGCCAATAC TGGCCACACC 
ATTCATGTTC ACTACCCTGG GAATCGCCAG CCCAACCCTC CTCTTATACT GCAGAGGTTG 
CTTGGTCCCT CAGCTGCTGC TGACATCCTT CAGCTGAGCA GCAGCCTTCC CCTACAAAGC 
CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC GATGACGTCC ACATCATCGC CCGTTCTGAT 
GATGAGCTGC TGGATGACTT TTTCCATGAT CAGAGCACAG CTACCAGCCA AGCAGGAACC 
CTGTCCAGCA TCCCCACAGC CCTGACCCGC TGGACAGAAG AATGCAAAGT TCTCGATGCT 
GAGAGCATGC ATGACTGTGT TTCAGTGGTT AAAGTGTCCA TTGTCAATCA CCTGGAATTC 
CTGAGGGATG AGGAGCTGGA AGAAAGGCGA GAGAAGCGCA GGAAACAACT GGCTGAGGAA 
GAAACAAAGA TAACTGATAA AGGCAAAGAA GATAAGGAGA ACAGGGATCA GAGTGCACAG 
TGTACTGCAT CTAAGTCAAA TGACTCCACT GAACAGAATC TCTCAGATGG GACGCCTATG 
CCTGACAGCT ACCCAACAAC CCCATCTTCA ACTGATGCAG CTACATCTGA GTCCAAGGAG 
ACCCTTGGCA CTCTGCAATC CTCACAACAG CAACCAACAC TCCCAACCCC ACCAGCTTTG 
GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA GCTGGAGAAG GGGGCAGCTC TACACAGCTA 
TTGATGCCTG TAGAGCCAGA GGAATTGGGT CCCACAAGGC CAAGTGGGGA AGCAGAAACA 
ACTCAGATGG AGTTATCCCC AGCTCCCACT ATAACCTCAC TTTCCCCAGA GAGAGCTGAG 
GATTCTGATG CACTGACGGC TGTCAGCAGT CAGCTAGAAG GCTCTCCTAT GGATACAAGC 
AGCCTGGCTT CCTGTACCTT AGAGGAGGCT GTGGGTGACA CTTCAGCAGC TGGCAGTTCT 
GAGCAGCCCA GAGCAGGCAG CTCCACTCCT GGGGATGCCC CACCAGCTGT GGCGGAAGTG 
CAAGGCAGGA GTGATGGGTC AGGGGAATCT GCCCAGCCAC CTGAGGACAG CTCCCCACCT 
GCATCCTCTG AGAGCTCTTC CACCAGAGAT TCTGCCGTGG CCATTTCTGG AGCAGATTCC 
CGAGGAATCC TAGAAGAGCC GTTGCCTTCA ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG 
GGTATCAGTC TCCCTGAAGG TGTGGACCCC TCTTTTCTGG CTGCCCTGCC TGATGACATC 
CGTCGGGAAG TTCTACAGAA CCAGCTAGGC ATTCGTCCAC CAACCCGGAC TGCCCCCTCC 
ACAAATAGCT CAGCGCCTGC AGTGGTGGGG AATCCTGGTG TGACTGAAGT GAGCCCTGAG 
TTTCTGGCTG CCCTGCCTCC AGCCATTCAG GAGGAAGTAC TGGCACAGCA GAGAGCTGAG 
CAGCAGCGAC GAGAACTAGC ACAGAATGCC AGCTCAGACA CCCCTATGGA CCCTGTGACC 
TTCATCCAGA CTCTGCCCTC AGACCTGCGC CGTAGTGTCC TAGAGGATAT GGAGGACAGT 
GTGTTAGCTG TGATGCCACC TGACATTGCA GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA 
GAAGCCCGGC AGCGACAGCT CATGCATGAG CGTCTGTTTG GGCACAGTAG CACCTCCGCA 
CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC 
CAGTATACTC GCCTTGCTGT GCAGAGAGGT GGCACCTTCC AGATGGGGGG TAGCAGCAGC 
CATAACAGGC CTTCTGGCAG TAATGTAGAT ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT 
CTGGACCACG AAGCCCTTTC TTGTCTCTTG GTCCTACTTT TTGTGGATGA GCCAAAGCTC 
AATACTAGCC GTCTACACCG AGTACTGAGA AATCTCTGCT ACCATGCCCA GACCCGCCAC 
TGGGTCATCC GCAGTCTGCT CTCCATCTTG CAGCGCAGCA GTGAGAGTGA GCTATGCATT 
GAAACACCCA AACTCACTAC AAGTGAGGAA AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG 
TCAAGTAGCC ATGAGAACCG TCCCCTGGAC CTGCTACACA AGATGGAGTC AAAGAGCTCC 
AACCAGCTTT CCTGGCTCTC AGTATCCATG GATGCAGCCC TAGGCTGCAG GACTAATATA 
TTTCAGATCC AGCGTTCAGG GGGGCGTAAA CATACCGAGA AGCATGCAAG CGGTGGCTCC 
ACCGTCCACA TCCATCCCCA AGCTGCTCCT GTTGTCTGCA GACACGTTTT GGATACACTC 
ATTCAATTGG CCAAGGTATT TCCCAGCCAC TTCACACAGC AGCGGACCAA AGAAACAAAC 
TGTGAGAGTG ATCGGGAAAG GGGCAATAAG GCCTGTAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TTTGCACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTC 
AGCCGGAAAG GCAAGAACTC CGTGAAGTCA GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA 
ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAC 
CCAGTCATCC GCCGGAGCTC TCTCTTAACT GAGAAACTCC TCAGACTCCT TTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAGGTGTCA GAAGCACAGG CTAATTCTGG CAGCGGTGCT 
TCCTCCACCA CCACTGCCAC CTCAACCACA TCTACCACCA CCACCACTGC CGCCTCCACC 
ACGCCCACAC CCCCTACTGC ACCCACCCCT GTCACTTCTG CTCCAGCCCT GGTTGCTGCC 
ACGGCTATTT CCACCATTGT CGTAGCTGCT TCGACCACAG TGACTACCCC CACGACTGCT 
ACCACTACTG TTTCAATTTC TCCCACTACT AAGGGCAGCA AATCTCCAGC GAAGGTGAGT 
GATGGGGGCA GCAGCAGTAC AGACTTTAAG ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAGGAAGG CTTAGAGGAT 
GCAGCCAACG TACTACTGCA GCTCTCCCGG GGGGACTCTG GGACCCGGGA CACTGTTCTC 
AAGCTGCTAC TGAATGGAGC CCGCCATCTG GGTTATACCC TTTGTAAACA AATAGGTACC 
CTGCTGGCCG AGCTGCGGGA ATACAACCTC GAGCAGCAGC GGCGAGCCCA ATGTGAAACC 
CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG CCACAGACCA CCAAGCTGAA GGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAGAATGTG GTAATTGTGG CATCTCAGAA GCGACCTTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTCC GGGACGACAC GCGCCGGGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTAG CCAGTCAGAG 
GCGTCTGTCC GGAGGGAGGA ATCACCCATG GATGTGGACC AGCCATCTCC CAGTGCTCAA 
GATACTCAAT CCATTGCCTC CGATGGAACC CCACAGGGGG AGAAGGAAAA GGAAGAAAGA 
CCACCTGAGT TACCCCTGCT CAGCGAGCAG CTGAGTTTGG ACGAGCTGTG GGACATGCTT 
GGGGAGTGTC TAAAGGAACT AGAGGAATCC CATGACCAGC ATGCGGTGCT AGTGCTACAG 
CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT GCCACAGAGC GGGAGAGCAA GCCTCCTGTC 
CGAGACACCC GTGAGAGCCA GCTGGCACAC ATCAAGGACG AGCCTCCTCC ACTCTCCCCT 
GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC CTTGACCCAT TCTTCTCCCG GGAGCCCTCA 
TCTATGCACA TCTCCTCAAG CCTGCCCCCT GACACACAGA AGTTCCTTCG CTTTGCAGAG 
ACTCACCGCA CTGTGTTAAA CCAGATCCTA CGGCAGTCCA CGACCCACCT TGCTGATGGG 
CCTTTTGCTG TCCTGGTAGA CTACATTCGT GTCCTCGACT TTGATGTCAA GCGCAAATAT 
TTCCGCCAAG AGCTGGAGCG TTTAGATGAG GGGCTCCGGA AAGAAGACAT GGCTGTGCAT 
GTCCGTCGTG ACCATGTGTT TGAAGACTCC TATCGTGAGC TGCATCGCAA ATCCCCCGAA 
GAAATGAAGA ATCGATTGTA TATAGTATTT GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG 
CTCCTGCGGG AGTGGTATAT GATCATCTCT CGAGAGATGT TTAACCCTAT GTATGCCTTG 
TTCCGTACCT CACCTGGTGA TCGAGTCACC TACACCATCA ATCCATCTTC CCACTGCAAC 
CCCAACCACC TCAGCTACTT CAAGTTTGTC GGACGCATTG TGGCCAAAGC TGTATATGAC 
AACCGTCTTC TGGAGTGCTA CTTTACTCGA TCCTTTTACA AACACATCTT GGGCAAGTCA 
GTCAGATATA CAGATATGGA GAGTGAAGAT TACCACTTCT ACCAAGGTCT GGTTTATCTG 
CTGGAAAATG ATGTCTCCAC ACTAGGCTAT GACCTCACCT TCAGCACTGA GGTCCAAGAG 
TTTGGAGTTT GTGAAGTTCG TGACCTCAAA CCCAATGGGG CCAACATCTT GGTAACAGAG 
GAGAATAAGA AGGAGTATGT ACACCTGGTA TGCCAGATGA GAATGACAGG AGCCATCCGC 
AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC TATGAGATCA TTCCAAAGCG CCTCATTTCC 
ATCTTCACTG AGCAGGAGTT AGAGCTGCTT ATATCAGGAC TGCCCACCAT TGACATCGAT 
GATCTGAAAT CCAACACTGA ATACCACAAG TACCAGTCCA ACTCTATTCA GATCCAGTGG 
TTCTGGAGAG CATTGCGTTC TTTCGATCAA GCTGACCGTG CCAAGTTCCT CCAGTTTGTC 
ACAGGTACTT CCAAGGTACC CCTGCAAGGC TTTGCTGCCC TCGAAGGCAT GAATGGCATT 
CAGAAGTTTC AGATCCATCG AGATGACAGG TCCACAGATC GCCTGCCTTC AGCTCACACA 
TGTTTTAATC AGCTGGATCT GCCTGCCTAT GAGAGCTTTG AGAAGCTCCG CCACATGCTA 
CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_005261965.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005262022.1 Homo sapiens HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X18, mRNA.

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CloneID	OHu23451
Clone ID Related Accession (Same CDS sequence)	XM_005261965.3 , XM_005261965.4 , NM_031407.6 , NM_031407.7
Accession Version	XM_005261965.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005261965.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAATCAGC AAACAGTATC 
TTGTATAATG GCTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACGGA TAAGCAGCTT 
ATGGAGATTA AAGCAGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT 
CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCA 
GTGCTTGTAA GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATACCCTCAC 
CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC 
GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGATA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT 
GAGCATGAAG TAGATTTGTG CCGAAAAGAA TGTCCGTTTG TGATCAAGCC AAAGATCCAG 
AGACCCAATA CTACACAAGA AGGAGAGGAA ATGGAAACTG ATATGGATGG AGTCCAGTGT 
ATTCCACAAC GAGCAGCACT TCTGAAATCC ATGTTGAATT TCCTCAAGAA GGCCATCCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTCTGCC TACCTCCCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGCCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAACCA TCACTGCTCT CCTCACTCCA GGACAATGGA 
TTGACAGATG TCATGCTGCA TGCACTGCTT ATCAAAGATG TTCCTGCTAC CCGTGAAGTC 
CTTGGCTCCC TCCCAAATGT ATTCAGTGCA CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTTCAGT GTCAGCCTTT TGAACGCCTC TTCAAAGTTC TTCTGTCTCC AGATTACCTC 
CCAGCCATGC GGAGGAGGAG AAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTCG ATGAGCTCAT GAGACATCAG CCCACCCTTA AAACAGATGC AACGACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGGG ACCCCAAATA CATCTGTCAG 
AAGCCATCAA TCCAGAAGGC AGATGGCACT GCCACTGCTC CTCCCCCAAG GTCTAATCAT 
GCCGCAGAAG AAGCCTCTAG TGAGGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTA CCCAGCAAAA TGAAACTGAG CCTAATCAGC AGGTTGTTGG TACAGAGGAA 
CGTATTCCTA TTCCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
CTGAGCAACA ATACAACAGA TGACCACTGC CAGGAATTTG TGAATCAGAA AGGACTGTTG 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACAC TGTCACATGA ACCCAAAGTC 
CTTCAAGAGG GTCTCCTTCA GTTGGACTCC ATCCTCTCCT CCCTGGAGCC CTTACACCGC 
CCCATTGAAT CCCCTGGGGG CTCAGTGTTG TTGCGAGAAC TGGCTTGCGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC CCAGGCCACA CCTCTGCTGC ATGCACTCAC TGCTGCCCAT 
GCCTACATCA TGATGTTTGT TCATACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCCATC 
TCCGTAAACC AGTGGGGCTC TCAATTGGGT CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA 
TACTGTTCCC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TGTGTACCCC AAACAGCCTA 
CCATCTGGGT GTGAATTTGG CCAGGCAGAT ATGCAGAAAC TGGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGGGAAC AGGATGGAGC AGCTGGAAGT 
ATGGATGCTT CTACCCAGGG CTTATTAGAA GGCATTGGGC TAGATGGTGA CACATTGGCT 
CCCATGGAGA CAGATGAACC TACTGCTTCA GACTCTAAGG GCAAATCTAA AATCACACCA 
GCAATGGCTG CCAGAATTAA GCAAATCAAG CCTTTGTTAT CAGCTTCCTC CAGATTAGGC 
CGAGCACTTG CTGAGCTATT TGGACTTCTT GTTAAACTTT GTGTGGGATC TCCTGTCCGC 
CAGAGAAGGA GCCATCATGC TGCCAGCACC ACTACAGCAC CGACACCTGC CGCGCGATCA 
ACAGCCTCAG CTCTCACTAA GCTCTTGACT AAGGGGTTAT CTTGGCAGCC CCCACCATAT 
ACACCTACTC CCCGATTCAG GCTGACATTC TTCATCTGTT CAGTTGGTTT CACATCCCCA 
ATGCTGTTTG ATGAGAGGAA GTATCCCTAC CACCTCATGC TGCAAAAATT TCTCTGCTCC 
GGAGGCCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TGTCCATGGG AGGTAAAGTT 
CCTGTTTCTG AGGGATTGGA ACACTCAGAC TTGCCTGATG GCACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTGGTGGA GAAGATGGTG AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGCTGCCTG CCAAATTGCC TGGAGGTGTC CAGAACTTTC CCCAGTTCAG TGCACTGCGC 
TTCCTTGTGG TAACTCAGAA AGCAGCCTTT ACTTGCATCA AAAACTTATG GAACCGGAAA 
CCCCTGAAGG TATATGGTGG ACGAATGGCT GAATCGATGC TGGCCATTCT ATGCCACATC 
CTCCGAGGAG AACCTGTGAT TCGAGAGAGA CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA 
GAAGAGGATA CAGGGCAAGA GGAAGGTGGC TCCCGCCGGG AACCTCAAGT CAACCAGCAA 
CAACTGCAAC AGCTCATGGA CATGGGCTTC ACAAGGGAAC ATGCAATGGA GGCACTGTTG 
AACACCAGCA CCATGGAGCA GGCCACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATG 
GGAGGAGTTG TTCGGGATCT CAGCATGTCT GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA 
TGTCTAGAGA AGTTCCAGGA TGCTGACCCG TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACTATGT TGCCAGGCTG CTTCCACCTT CTTGATGAGC TGCCAGACAC AGTATACCGT 
GTGTGTGACC TGATCATGAC AGCAATCAAA CGTAATGGAG CAGATTATCG TGACATGATT 
CTGAAGCAAG TAGTCAATCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCCCTGACAA CAAGTGACAC AAAAACCGTG TCAGAGTGGA TAAGTCAGAT GGCCACACTG 
CCCCAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CGCTACTTTT TGAGGAGTTG 
AAGCTACCTT GTGCTTGGGT GGTTGAATCA AGTGGCATCC TTAATGTCCT AATCAAACTC 
TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA GCCAAGGAGC AGAAGGAAGT CCAGACCCCA 
AAGTGGATCA CACCAGTGTT GCTCCTGATT GATTTCTATG AAAAGACAGC CATCTCCTCA 
AAAAGGAGAG CCCAGATGAC TAAGTACCTG CAATCCAACA GCAACAACTG GCGCTGGTTT 
GATGATCGCT CTGGGCGTTG GTGTAGTTAC AGTGCAAGCA ACAATAGCAC TATTGATTCT 
GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA TTCACTGCAG GCCGAAGAAG ATACACGGTC 
CAATTCACTA CAATGGTGCA GGTTAATGAG GAAACAGGGA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTACCTCG GCTGAATAAA AATTCAAAAA ACAGCAATGG ACAGGAACTA 
GAGAAGACGC TGGAAGAAAG CAAAGAAATG GATATCAAAC GTAAAGAAAA TAAAGGCAAT 
GATACCCCTT TGGCCCTAGA GAGTACAAAC ACTGAAAAGG AGACAAGCCT GGAGGAAACA 
AAAATCGGGG AGATCCTGAT CCAGGGCTTG ACAGAAGATA TGGTGACTGT TTTAATCCGG 
GCCTGCGTGA GCATGCTGGG AGTCCCTGTG GACCCAGATA CTTTGCATGC CACCCTTCGT 
CTCTGTCTGA GGCTCACCCG GGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACCCGCATGA TCTTGAATTT GACCCAGAGC TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC 
ACCCTTCTCT TAAGACACAT CATTGAGGAC CCCTGTACCC TTCGTCATAC CATGGAAAAG 
GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT GGTAGCACTA CCTCTGGTGT TGTGTCTGGC 
AGCCTCGGCT CTCGGGAGAT CAACTACATC CTTCGTGTCC TTGGGCCAGC CGCATGCCGC 
AATCCAGACA TATTCACAGA AGTGGCCAAC TGCTGTATCC GCATCGCCCT TCCTGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGCCCTAAT 
GCTGTACAGC TGGTGAAGAC CACCCCTTTG AAGCCCTCAC CTCTGCCTGT CATCCCTGAT 
ACTATCAAGG AAGTGATCTA TGATATGCTG AATGCTCTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGATA AATCTGATCC TAAACCTGGG GTTATGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGATGATGT ATACCAGCAG TACCGGTCAC TTACGCGTCA GAGCAGTGAC 
TTTGATACGC AGTCAGGTTT TTCCATTAAT AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC 
ACTGAGACTT CCGCATCTGG GACCTCCCAA GGAGAGGCTT CAACTCCAGA GGAGTCTCGA 
GATGGGAAGA AAGATAAAGA AGGGGACCGG GCCTCTGAGG AAGGCAAACA GAAAGGCAAG 
GGCAGCAAAC CTTTAATGCC TACCTCCACT ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG 
TCCTATGTTG GTATTGCTAC CCTGATTGCC AACTACAGCT ACACTGTGGG CCAGTCTGAA 
CTGATCAAAG AGGACTGCAG TGTGCTAGCT TTTGTTCTGG ACCACCTGCT CCCACATACC 
CAGAATGCAG AAGACAAGGA CACCCCTGCC TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT 
GCTGCAGGGA GTGGCACAGA TGCCCAGGTG GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT 
GGACGGGCAC TGGCTATGGC TGAGAGTACA GAGAAACATG CCAGGCTTCA GGCAGTGATG 
TGTATCATCA GTACTATCAT GGAGTCCTGC CCCTCCACCT CCAGCTTCTA CAGCAGTGCC 
ACAGCGAAGA CCCAGCACAA TGGCATGAAC AACATCATTC GGCTTTTCCT GAAGAAGGGA 
CTGGTTAATG ACCTGGCCAG AGTACCTCAC AGCTTAGACC TGTCCAGTCC CAACATGGCC 
AACACAGTCA ATGCTGCTCT GAAGCCTTTG GAAACACTTT CCCGGATTGT GAACCAGCCC 
AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT AGCAAGAACA AGTCTGAGCA GGATGCCCAA 
GGAGCCTCTC AAGATTCCAG TAGCAACCAG CAGGACCCAG GCGAGCCTGG GGAAGCAGAA 
GTGCAGGAGG AGGATCATGA TGTCACTCAG ACAGAGGTGG CAGATGGGGA TATCATGGAT 
GGGGAGGCTG AAACCGACTC AGTGGTGATT GCTGGGCAGC CTGAGGTGCT CAGTTCACAA 
GAGATGCAGG TTGAGAATGA GCTGGAGGAC CTGATAGATG AGTTGCTTGA GAGGGATGGC 
GGATCTGGGA ACAGTACAAT TATAGTGAGC AGAAGTGGAG AGGATGAATC ACAAGAGGAC 
GTGCTGATGG ATGAAGCTCC TTCCAACCTC AGCCAAGCTT CCACCTTGCA GGCCAACCGA 
GAAGATTCCA TGAATATCCT GGACCCTGAG GATGAGGAGG AGCACACTCA GGAAGAGGAC 
AGCAGTGGCA GTAACGAGGA TGAGGATGAT AGTCAGGATG AAGAGGAGGA GGAGGAGGAA 
GATGAGGAAG ATGATCAGGA GGATGATGAA GGTGAAGAGG GAGATGAAGA CGATGACGAC 
GATGGCTCTG AGATGGAATT GGATGAGGAT TATCCTGATA TGAACGCTTC TCCCTTGGTC 
CGATTTGAGC GCTTTGACCG GGAGGATGAT CTCATCATTG AGTTTGACAA CATGTTCTCC 
AGTGCTACAG ACATCCCCCC ATCCCCAGGA AATATCCCTA CCACCCATCC ACTGATGGTG 
CGCCATGCAG ACCACAGTTC TCTGACACTG GGCAGTGGCT CTTCAACAAC TCGTCTCACC 
CAGGGCATCG GGCGCAGTCA GAGGACCCTA AGGCAGCTGA CGGCCAATAC TGGCCACACC 
ATTCATGTTC ACTACCCTGG GAATCGCCAG CCCAACCCTC CTCTTATACT GCAGAGGTTG 
CTTGGTCCCT CAGCTGCTGC TGACATCCTT CAGCTGAGCA GCAGCCTTCC CCTACAAAGC 
CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC GATGACGTCC ACATCATCGC CCGTTCTGAT 
GATGAGCTGC TGGATGACTT TTTCCATGAT CAGAGCACAG CTACCAGCCA AGCAGGAACC 
CTGTCCAGCA TCCCCACAGC CCTGACCCGC TGGACAGAAG AATGCAAAGT TCTCGATGCT 
GAGAGCATGC ATGACTGTGT TTCAGTGGTT AAAGTGTCCA TTGTCAATCA CCTGGAATTC 
CTGAGGGATG AGGAGCTGGA AGAAAGGCGA GAGAAGCGCA GGAAACAACT GGCTGAGGAA 
GAAACAAAGA TAACTGATAA AGGCAAAGAA GATAAGGAGA ACAGGGATCA GAGTGCACAG 
TGTACTGCAT CTAAGTCAAA TGACTCCACT GAACAGAATC TCTCAGATGG GACGCCTATG 
CCTGACAGCT ACCCAACAAC CCCATCTTCA ACTGATGCAG CTACATCTGA GTCCAAGGAG 
ACCCTTGGCA CTCTGCAATC CTCACAACAG CAACCAACAC TCCCAACCCC ACCAGCTTTG 
GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA GCTGGAGAAG GGGGCAGCTC TACACAGCTA 
TTGATGCCTG TAGAGCCAGA GGAATTGGGT CCCACAAGGC CAAGTGGGGA AGCAGAAACA 
ACTCAGATGG AGTTATCCCC AGCTCCCACT ATAACCTCAC TTTCCCCAGA GAGAGCTGAG 
GATTCTGATG CACTGACGGC TGTCAGCAGT CAGCTAGAAG GCTCTCCTAT GGATACAAGC 
AGCCTGGCTT CCTGTACCTT AGAGGAGGCT GTGGGTGACA CTTCAGCAGC TGGCAGTTCT 
GAGCAGCCCA GAGCAGGCAG CTCCACTCCT GGGGATGCCC CACCAGCTGT GGCGGAAGTG 
CAAGGCAGGA GTGATGGGTC AGGGGAATCT GCCCAGCCAC CTGAGGACAG CTCCCCACCT 
GCATCCTCTG AGAGCTCTTC CACCAGAGAT TCTGCCGTGG CCATTTCTGG AGCAGATTCC 
CGAGGAATCC TAGAAGAGCC GTTGCCTTCA ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG 
GGTATCAGTC TCCCTGAAGG TGTGGACCCC TCTTTTCTGG CTGCCCTGCC TGATGACATC 
CGTCGGGAAG TTCTACAGAA CCAGCTAGGC ATTCGTCCAC CAACCCGGAC TGCCCCCTCC 
ACAAATAGCT CAGCGCCTGC AGTGGTGGGG AATCCTGGTG TGACTGAAGT GAGCCCTGAG 
TTTCTGGCTG CCCTGCCTCC AGCCATTCAG GAGGAAGTAC TGGCACAGCA GAGAGCTGAG 
CAGCAGCGAC GAGAACTAGC ACAGAATGCC AGCTCAGACA CCCCTATGGA CCCTGTGACC 
TTCATCCAGA CTCTGCCCTC AGACCTGCGC CGTAGTGTCC TAGAGGATAT GGAGGACAGT 
GTGTTAGCTG TGATGCCACC TGACATTGCA GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA 
GAAGCCCGGC AGCGACAGCT CATGCATGAG CGTCTGTTTG GGCACAGTAG CACCTCCGCA 
CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC 
CAGTATACTC GCCTTGCTGT GCAGAGAGGT GGCACCTTCC AGATGGGGGG TAGCAGCAGC 
CATAACAGGC CTTCTGGCAG TAATGTAGAT ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT 
CTGGACCACG AAGCCCTTTC TTGTCTCTTG GTCCTACTTT TTGTGGATGA GCCAAAGCTC 
AATACTAGCC GTCTACACCG AGTACTGAGA AATCTCTGCT ACCATGCCCA GACCCGCCAC 
TGGGTCATCC GCAGTCTGCT CTCCATCTTG CAGCGCAGCA GTGAGAGTGA GCTATGCATT 
GAAACACCCA AACTCACTAC AAGTGAGGAA AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG 
TCAAGTAGCC ATGAGAACCG TCCCCTGGAC CTGCTACACA AGATGGAGTC AAAGAGCTCC 
AACCAGCTTT CCTGGCTCTC AGTATCCATG GATGCAGCCC TAGGCTGCAG GACTAATATA 
TTTCAGATCC AGCGTTCAGG GGGGCGTAAA CATACCGAGA AGCATGCAAG CGGTGGCTCC 
ACCGTCCACA TCCATCCCCA AGCTGCTCCT GTTGTCTGCA GACACGTTTT GGATACACTC 
ATTCAATTGG CCAAGGTATT TCCCAGCCAC TTCACACAGC AGCGGACCAA AGAAACAAAC 
TGTGAGAGTG ATCGGGAAAG GGGCAATAAG GCCTGTAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TTTGCACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTC 
AGCCGGAAAG GCAAGAACTC CGTGAAGTCA GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA 
ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAC 
CCAGTCATCC GCCGGAGCTC TCTCTTAACT GAGAAACTCC TCAGACTCCT TTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAGGTGTCA GAAGCACAGG CTAATTCTGG CAGCGGTGCT 
TCCTCCACCA CCACTGCCAC CTCAACCACA TCTACCACCA CCACCACTGC CGCCTCCACC 
ACGCCCACAC CCCCTACTGC ACCCACCCCT GTCACTTCTG CTCCAGCCCT GGTTGCTGCC 
ACGGCTATTT CCACCATTGT CGTAGCTGCT TCGACCACAG TGACTACCCC CACGACTGCT 
ACCACTACTG TTTCAATTTC TCCCACTACT AAGGGCAGCA AATCTCCAGC GAAGGTGAGT 
GATGGGGGCA GCAGCAGTAC AGACTTTAAG ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAGGAAGG CTTAGAGGAT 
GCAGCCAACG TACTACTGCA GCTCTCCCGG GGGGACTCTG GGACCCGGGA CACTGTTCTC 
AAGCTGCTAC TGAATGGAGC CCGCCATCTG GGTTATACCC TTTGTAAACA AATAGGTACC 
CTGCTGGCCG AGCTGCGGGA ATACAACCTC GAGCAGCAGC GGCGAGCCCA ATGTGAAACC 
CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG CCACAGACCA CCAAGCTGAA GGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAGAATGTG GTAATTGTGG CATCTCAGAA GCGACCTTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTCC GGGACGACAC GCGCCGGGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTAG CCAGTCAGAG 
GCGTCTGTCC GGAGGGAGGA ATCACCCATG GATGTGGACC AGCCATCTCC CAGTGCTCAA 
GATACTCAAT CCATTGCCTC CGATGGAACC CCACAGGGGG AGAAGGAAAA GGAAGAAAGA 
CCACCTGAGT TACCCCTGCT CAGCGAGCAG CTGAGTTTGG ACGAGCTGTG GGACATGCTT 
GGGGAGTGTC TAAAGGAACT AGAGGAATCC CATGACCAGC ATGCGGTGCT AGTGCTACAG 
CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT GCCACAGAGC GGGAGAGCAA GCCTCCTGTC 
CGAGACACCC GTGAGAGCCA GCTGGCACAC ATCAAGGACG AGCCTCCTCC ACTCTCCCCT 
GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC CTTGACCCAT TCTTCTCCCG GGAGCCCTCA 
TCTATGCACA TCTCCTCAAG CCTGCCCCCT GACACACAGA AGTTCCTTCG CTTTGCAGAG 
ACTCACCGCA CTGTGTTAAA CCAGATCCTA CGGCAGTCCA CGACCCACCT TGCTGATGGG 
CCTTTTGCTG TCCTGGTAGA CTACATTCGT GTCCTCGACT TTGATGTCAA GCGCAAATAT 
TTCCGCCAAG AGCTGGAGCG TTTAGATGAG GGGCTCCGGA AAGAAGACAT GGCTGTGCAT 
GTCCGTCGTG ACCATGTGTT TGAAGACTCC TATCGTGAGC TGCATCGCAA ATCCCCCGAA 
GAAATGAAGA ATCGATTGTA TATAGTATTT GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG 
CTCCTGCGGG AGTGGTATAT GATCATCTCT CGAGAGATGT TTAACCCTAT GTATGCCTTG 
TTCCGTACCT CACCTGGTGA TCGAGTCACC TACACCATCA ATCCATCTTC CCACTGCAAC 
CCCAACCACC TCAGCTACTT CAAGTTTGTC GGACGCATTG TGGCCAAAGC TGTATATGAC 
AACCGTCTTC TGGAGTGCTA CTTTACTCGA TCCTTTTACA AACACATCTT GGGCAAGTCA 
GTCAGATATA CAGATATGGA GAGTGAAGAT TACCACTTCT ACCAAGGTCT GGTTTATCTG 
CTGGAAAATG ATGTCTCCAC ACTAGGCTAT GACCTCACCT TCAGCACTGA GGTCCAAGAG 
TTTGGAGTTT GTGAAGTTCG TGACCTCAAA CCCAATGGGG CCAACATCTT GGTAACAGAG 
GAGAATAAGA AGGAGTATGT ACACCTGGTA TGCCAGATGA GAATGACAGG AGCCATCCGC 
AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC TATGAGATCA TTCCAAAGCG CCTCATTTCC 
ATCTTCACTG AGCAGGAGTT AGAGCTGCTT ATATCAGGAC TGCCCACCAT TGACATCGAT 
GATCTGAAAT CCAACACTGA ATACCACAAG TACCAGTCCA ACTCTATTCA GATCCAGTGG 
TTCTGGAGAG CATTGCGTTC TTTCGATCAA GCTGACCGTG CCAAGTTCCT CCAGTTTGTC 
ACAGGTACTT CCAAGGTACC CCTGCAAGGC TTTGCTGCCC TCGAAGGCAT GAATGGCATT 
CAGAAGTTTC AGATCCATCG AGATGACAGG TCCACAGATC GCCTGCCTTC AGCTCACACA 
TGTTTTAATC AGCTGGATCT GCCTGCCTAT GAGAGCTTTG AGAAGCTCCG CCACATGCTA 
CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005262022.1
CDS	471..13595
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_005261965.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005261965.3

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAATCAGC AAACAGTATC 
TTGTATAATG GCTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACGGA TAAGCAGCTT 
ATGGAGATTA AAGCAGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT 
CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCA 
GTGCTTGTAA GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATACCCTCAC 
CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC 
GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGATA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT 
GAGCATGAAG TAGATTTGTG CCGAAAAGAA TGTCCGTTTG TGATCAAGCC AAAGATCCAG 
AGACCCAATA CTACACAAGA AGGAGAGGAA ATGGAAACTG ATATGGATGG AGTCCAGTGT 
ATTCCACAAC GAGCAGCACT TCTGAAATCC ATGTTGAATT TCCTCAAGAA GGCCATCCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTCTGCC TACCTCCCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGCCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAACCA TCACTGCTCT CCTCACTCCA GGACAATGGA 
TTGACAGATG TCATGCTGCA TGCACTGCTT ATCAAAGATG TTCCTGCTAC CCGTGAAGTC 
CTTGGCTCCC TCCCAAATGT ATTCAGTGCA CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTTCAGT GTCAGCCTTT TGAACGCCTC TTCAAAGTTC TTCTGTCTCC AGATTACCTC 
CCAGCCATGC GGAGGAGGAG AAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTCG ATGAGCTCAT GAGACATCAG CCCACCCTTA AAACAGATGC AACGACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGGG ACCCCAAATA CATCTGTCAG 
AAGCCATCAA TCCAGAAGGC AGATGGCACT GCCACTGCTC CTCCCCCAAG GTCTAATCAT 
GCCGCAGAAG AAGCCTCTAG TGAGGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTA CCCAGCAAAA TGAAACTGAG CCTAATCAGC AGGTTGTTGG TACAGAGGAA 
CGTATTCCTA TTCCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
CTGAGCAACA ATACAACAGA TGACCACTGC CAGGAATTTG TGAATCAGAA AGGACTGTTG 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACAC TGTCACATGA ACCCAAAGTC 
CTTCAAGAGG GTCTCCTTCA GTTGGACTCC ATCCTCTCCT CCCTGGAGCC CTTACACCGC 
CCCATTGAAT CCCCTGGGGG CTCAGTGTTG TTGCGAGAAC TGGCTTGCGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC CCAGGCCACA CCTCTGCTGC ATGCACTCAC TGCTGCCCAT 
GCCTACATCA TGATGTTTGT TCATACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCCATC 
TCCGTAAACC AGTGGGGCTC TCAATTGGGT CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA 
TACTGTTCCC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TGTGTACCCC AAACAGCCTA 
CCATCTGGGT GTGAATTTGG CCAGGCAGAT ATGCAGAAAC TGGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGGGAAC AGGATGGAGC AGCTGGAAGT 
ATGGATGCTT CTACCCAGGG CTTATTAGAA GGCATTGGGC TAGATGGTGA CACATTGGCT 
CCCATGGAGA CAGATGAACC TACTGCTTCA GACTCTAAGG GCAAATCTAA AATCACACCA 
GCAATGGCTG CCAGAATTAA GCAAATCAAG CCTTTGTTAT CAGCTTCCTC CAGATTAGGC 
CGAGCACTTG CTGAGCTATT TGGACTTCTT GTTAAACTTT GTGTGGGATC TCCTGTCCGC 
CAGAGAAGGA GCCATCATGC TGCCAGCACC ACTACAGCAC CGACACCTGC CGCGCGATCA 
ACAGCCTCAG CTCTCACTAA GCTCTTGACT AAGGGGTTAT CTTGGCAGCC CCCACCATAT 
ACACCTACTC CCCGATTCAG GCTGACATTC TTCATCTGTT CAGTTGGTTT CACATCCCCA 
ATGCTGTTTG ATGAGAGGAA GTATCCCTAC CACCTCATGC TGCAAAAATT TCTCTGCTCC 
GGAGGCCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TGTCCATGGG AGGTAAAGTT 
CCTGTTTCTG AGGGATTGGA ACACTCAGAC TTGCCTGATG GCACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTGGTGGA GAAGATGGTG AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGCTGCCTG CCAAATTGCC TGGAGGTGTC CAGAACTTTC CCCAGTTCAG TGCACTGCGC 
TTCCTTGTGG TAACTCAGAA AGCAGCCTTT ACTTGCATCA AAAACTTATG GAACCGGAAA 
CCCCTGAAGG TATATGGTGG ACGAATGGCT GAATCGATGC TGGCCATTCT ATGCCACATC 
CTCCGAGGAG AACCTGTGAT TCGAGAGAGA CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA 
GAAGAGGATA CAGGGCAAGA GGAAGGTGGC TCCCGCCGGG AACCTCAAGT CAACCAGCAA 
CAACTGCAAC AGCTCATGGA CATGGGCTTC ACAAGGGAAC ATGCAATGGA GGCACTGTTG 
AACACCAGCA CCATGGAGCA GGCCACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATG 
GGAGGAGTTG TTCGGGATCT CAGCATGTCT GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA 
TGTCTAGAGA AGTTCCAGGA TGCTGACCCG TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACTATGT TGCCAGGCTG CTTCCACCTT CTTGATGAGC TGCCAGACAC AGTATACCGT 
GTGTGTGACC TGATCATGAC AGCAATCAAA CGTAATGGAG CAGATTATCG TGACATGATT 
CTGAAGCAAG TAGTCAATCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCCCTGACAA CAAGTGACAC AAAAACCGTG TCAGAGTGGA TAAGTCAGAT GGCCACACTG 
CCCCAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CGCTACTTTT TGAGGAGTTG 
AAGCTACCTT GTGCTTGGGT GGTTGAATCA AGTGGCATCC TTAATGTCCT AATCAAACTC 
TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA GCCAAGGAGC AGAAGGAAGT CCAGACCCCA 
AAGTGGATCA CACCAGTGTT GCTCCTGATT GATTTCTATG AAAAGACAGC CATCTCCTCA 
AAAAGGAGAG CCCAGATGAC TAAGTACCTG CAATCCAACA GCAACAACTG GCGCTGGTTT 
GATGATCGCT CTGGGCGTTG GTGTAGTTAC AGTGCAAGCA ACAATAGCAC TATTGATTCT 
GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA TTCACTGCAG GCCGAAGAAG ATACACGGTC 
CAATTCACTA CAATGGTGCA GGTTAATGAG GAAACAGGGA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTACCTCG GCTGAATAAA AATTCAAAAA ACAGCAATGG ACAGGAACTA 
GAGAAGACGC TGGAAGAAAG CAAAGAAATG GATATCAAAC GTAAAGAAAA TAAAGGCAAT 
GATACCCCTT TGGCCCTAGA GAGTACAAAC ACTGAAAAGG AGACAAGCCT GGAGGAAACA 
AAAATCGGGG AGATCCTGAT CCAGGGCTTG ACAGAAGATA TGGTGACTGT TTTAATCCGG 
GCCTGCGTGA GCATGCTGGG AGTCCCTGTG GACCCAGATA CTTTGCATGC CACCCTTCGT 
CTCTGTCTGA GGCTCACCCG GGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACCCGCATGA TCTTGAATTT GACCCAGAGC TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC 
ACCCTTCTCT TAAGACACAT CATTGAGGAC CCCTGTACCC TTCGTCATAC CATGGAAAAG 
GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT GGTAGCACTA CCTCTGGTGT TGTGTCTGGC 
AGCCTCGGCT CTCGGGAGAT CAACTACATC CTTCGTGTCC TTGGGCCAGC CGCATGCCGC 
AATCCAGACA TATTCACAGA AGTGGCCAAC TGCTGTATCC GCATCGCCCT TCCTGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGCCCTAAT 
GCTGTACAGC TGGTGAAGAC CACCCCTTTG AAGCCCTCAC CTCTGCCTGT CATCCCTGAT 
ACTATCAAGG AAGTGATCTA TGATATGCTG AATGCTCTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGATA AATCTGATCC TAAACCTGGG GTTATGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGATGATGT ATACCAGCAG TACCGGTCAC TTACGCGTCA GAGCAGTGAC 
TTTGATACGC AGTCAGGTTT TTCCATTAAT AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC 
ACTGAGACTT CCGCATCTGG GACCTCCCAA GGAGAGGCTT CAACTCCAGA GGAGTCTCGA 
GATGGGAAGA AAGATAAAGA AGGGGACCGG GCCTCTGAGG AAGGCAAACA GAAAGGCAAG 
GGCAGCAAAC CTTTAATGCC TACCTCCACT ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG 
TCCTATGTTG GTATTGCTAC CCTGATTGCC AACTACAGCT ACACTGTGGG CCAGTCTGAA 
CTGATCAAAG AGGACTGCAG TGTGCTAGCT TTTGTTCTGG ACCACCTGCT CCCACATACC 
CAGAATGCAG AAGACAAGGA CACCCCTGCC TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT 
GCTGCAGGGA GTGGCACAGA TGCCCAGGTG GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT 
GGACGGGCAC TGGCTATGGC TGAGAGTACA GAGAAACATG CCAGGCTTCA GGCAGTGATG 
TGTATCATCA GTACTATCAT GGAGTCCTGC CCCTCCACCT CCAGCTTCTA CAGCAGTGCC 
ACAGCGAAGA CCCAGCACAA TGGCATGAAC AACATCATTC GGCTTTTCCT GAAGAAGGGA 
CTGGTTAATG ACCTGGCCAG AGTACCTCAC AGCTTAGACC TGTCCAGTCC CAACATGGCC 
AACACAGTCA ATGCTGCTCT GAAGCCTTTG GAAACACTTT CCCGGATTGT GAACCAGCCC 
AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT AGCAAGAACA AGTCTGAGCA GGATGCCCAA 
GGAGCCTCTC AAGATTCCAG TAGCAACCAG CAGGACCCAG GCGAGCCTGG GGAAGCAGAA 
GTGCAGGAGG AGGATCATGA TGTCACTCAG ACAGAGGTGG CAGATGGGGA TATCATGGAT 
GGGGAGGCTG AAACCGACTC AGTGGTGATT GCTGGGCAGC CTGAGGTGCT CAGTTCACAA 
GAGATGCAGG TTGAGAATGA GCTGGAGGAC CTGATAGATG AGTTGCTTGA GAGGGATGGC 
GGATCTGGGA ACAGTACAAT TATAGTGAGC AGAAGTGGAG AGGATGAATC ACAAGAGGAC 
GTGCTGATGG ATGAAGCTCC TTCCAACCTC AGCCAAGCTT CCACCTTGCA GGCCAACCGA 
GAAGATTCCA TGAATATCCT GGACCCTGAG GATGAGGAGG AGCACACTCA GGAAGAGGAC 
AGCAGTGGCA GTAACGAGGA TGAGGATGAT AGTCAGGATG AAGAGGAGGA GGAGGAGGAA 
GATGAGGAAG ATGATCAGGA GGATGATGAA GGTGAAGAGG GAGATGAAGA CGATGACGAC 
GATGGCTCTG AGATGGAATT GGATGAGGAT TATCCTGATA TGAACGCTTC TCCCTTGGTC 
CGATTTGAGC GCTTTGACCG GGAGGATGAT CTCATCATTG AGTTTGACAA CATGTTCTCC 
AGTGCTACAG ACATCCCCCC ATCCCCAGGA AATATCCCTA CCACCCATCC ACTGATGGTG 
CGCCATGCAG ACCACAGTTC TCTGACACTG GGCAGTGGCT CTTCAACAAC TCGTCTCACC 
CAGGGCATCG GGCGCAGTCA GAGGACCCTA AGGCAGCTGA CGGCCAATAC TGGCCACACC 
ATTCATGTTC ACTACCCTGG GAATCGCCAG CCCAACCCTC CTCTTATACT GCAGAGGTTG 
CTTGGTCCCT CAGCTGCTGC TGACATCCTT CAGCTGAGCA GCAGCCTTCC CCTACAAAGC 
CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC GATGACGTCC ACATCATCGC CCGTTCTGAT 
GATGAGCTGC TGGATGACTT TTTCCATGAT CAGAGCACAG CTACCAGCCA AGCAGGAACC 
CTGTCCAGCA TCCCCACAGC CCTGACCCGC TGGACAGAAG AATGCAAAGT TCTCGATGCT 
GAGAGCATGC ATGACTGTGT TTCAGTGGTT AAAGTGTCCA TTGTCAATCA CCTGGAATTC 
CTGAGGGATG AGGAGCTGGA AGAAAGGCGA GAGAAGCGCA GGAAACAACT GGCTGAGGAA 
GAAACAAAGA TAACTGATAA AGGCAAAGAA GATAAGGAGA ACAGGGATCA GAGTGCACAG 
TGTACTGCAT CTAAGTCAAA TGACTCCACT GAACAGAATC TCTCAGATGG GACGCCTATG 
CCTGACAGCT ACCCAACAAC CCCATCTTCA ACTGATGCAG CTACATCTGA GTCCAAGGAG 
ACCCTTGGCA CTCTGCAATC CTCACAACAG CAACCAACAC TCCCAACCCC ACCAGCTTTG 
GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA GCTGGAGAAG GGGGCAGCTC TACACAGCTA 
TTGATGCCTG TAGAGCCAGA GGAATTGGGT CCCACAAGGC CAAGTGGGGA AGCAGAAACA 
ACTCAGATGG AGTTATCCCC AGCTCCCACT ATAACCTCAC TTTCCCCAGA GAGAGCTGAG 
GATTCTGATG CACTGACGGC TGTCAGCAGT CAGCTAGAAG GCTCTCCTAT GGATACAAGC 
AGCCTGGCTT CCTGTACCTT AGAGGAGGCT GTGGGTGACA CTTCAGCAGC TGGCAGTTCT 
GAGCAGCCCA GAGCAGGCAG CTCCACTCCT GGGGATGCCC CACCAGCTGT GGCGGAAGTG 
CAAGGCAGGA GTGATGGGTC AGGGGAATCT GCCCAGCCAC CTGAGGACAG CTCCCCACCT 
GCATCCTCTG AGAGCTCTTC CACCAGAGAT TCTGCCGTGG CCATTTCTGG AGCAGATTCC 
CGAGGAATCC TAGAAGAGCC GTTGCCTTCA ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG 
GGTATCAGTC TCCCTGAAGG TGTGGACCCC TCTTTTCTGG CTGCCCTGCC TGATGACATC 
CGTCGGGAAG TTCTACAGAA CCAGCTAGGC ATTCGTCCAC CAACCCGGAC TGCCCCCTCC 
ACAAATAGCT CAGCGCCTGC AGTGGTGGGG AATCCTGGTG TGACTGAAGT GAGCCCTGAG 
TTTCTGGCTG CCCTGCCTCC AGCCATTCAG GAGGAAGTAC TGGCACAGCA GAGAGCTGAG 
CAGCAGCGAC GAGAACTAGC ACAGAATGCC AGCTCAGACA CCCCTATGGA CCCTGTGACC 
TTCATCCAGA CTCTGCCCTC AGACCTGCGC CGTAGTGTCC TAGAGGATAT GGAGGACAGT 
GTGTTAGCTG TGATGCCACC TGACATTGCA GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA 
GAAGCCCGGC AGCGACAGCT CATGCATGAG CGTCTGTTTG GGCACAGTAG CACCTCCGCA 
CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC 
CAGTATACTC GCCTTGCTGT GCAGAGAGGT GGCACCTTCC AGATGGGGGG TAGCAGCAGC 
CATAACAGGC CTTCTGGCAG TAATGTAGAT ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT 
CTGGACCACG AAGCCCTTTC TTGTCTCTTG GTCCTACTTT TTGTGGATGA GCCAAAGCTC 
AATACTAGCC GTCTACACCG AGTACTGAGA AATCTCTGCT ACCATGCCCA GACCCGCCAC 
TGGGTCATCC GCAGTCTGCT CTCCATCTTG CAGCGCAGCA GTGAGAGTGA GCTATGCATT 
GAAACACCCA AACTCACTAC AAGTGAGGAA AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG 
TCAAGTAGCC ATGAGAACCG TCCCCTGGAC CTGCTACACA AGATGGAGTC AAAGAGCTCC 
AACCAGCTTT CCTGGCTCTC AGTATCCATG GATGCAGCCC TAGGCTGCAG GACTAATATA 
TTTCAGATCC AGCGTTCAGG GGGGCGTAAA CATACCGAGA AGCATGCAAG CGGTGGCTCC 
ACCGTCCACA TCCATCCCCA AGCTGCTCCT GTTGTCTGCA GACACGTTTT GGATACACTC 
ATTCAATTGG CCAAGGTATT TCCCAGCCAC TTCACACAGC AGCGGACCAA AGAAACAAAC 
TGTGAGAGTG ATCGGGAAAG GGGCAATAAG GCCTGTAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TTTGCACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTC 
AGCCGGAAAG GCAAGAACTC CGTGAAGTCA GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA 
ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAC 
CCAGTCATCC GCCGGAGCTC TCTCTTAACT GAGAAACTCC TCAGACTCCT TTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAGGTGTCA GAAGCACAGG CTAATTCTGG CAGCGGTGCT 
TCCTCCACCA CCACTGCCAC CTCAACCACA TCTACCACCA CCACCACTGC CGCCTCCACC 
ACGCCCACAC CCCCTACTGC ACCCACCCCT GTCACTTCTG CTCCAGCCCT GGTTGCTGCC 
ACGGCTATTT CCACCATTGT CGTAGCTGCT TCGACCACAG TGACTACCCC CACGACTGCT 
ACCACTACTG TTTCAATTTC TCCCACTACT AAGGGCAGCA AATCTCCAGC GAAGGTGAGT 
GATGGGGGCA GCAGCAGTAC AGACTTTAAG ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAGGAAGG CTTAGAGGAT 
GCAGCCAACG TACTACTGCA GCTCTCCCGG GGGGACTCTG GGACCCGGGA CACTGTTCTC 
AAGCTGCTAC TGAATGGAGC CCGCCATCTG GGTTATACCC TTTGTAAACA AATAGGTACC 
CTGCTGGCCG AGCTGCGGGA ATACAACCTC GAGCAGCAGC GGCGAGCCCA ATGTGAAACC 
CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG CCACAGACCA CCAAGCTGAA GGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAGAATGTG GTAATTGTGG CATCTCAGAA GCGACCTTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTCC GGGACGACAC GCGCCGGGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTAG CCAGTCAGAG 
GCGTCTGTCC GGAGGGAGGA ATCACCCATG GATGTGGACC AGCCATCTCC CAGTGCTCAA 
GATACTCAAT CCATTGCCTC CGATGGAACC CCACAGGGGG AGAAGGAAAA GGAAGAAAGA 
CCACCTGAGT TACCCCTGCT CAGCGAGCAG CTGAGTTTGG ACGAGCTGTG GGACATGCTT 
GGGGAGTGTC TAAAGGAACT AGAGGAATCC CATGACCAGC ATGCGGTGCT AGTGCTACAG 
CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT GCCACAGAGC GGGAGAGCAA GCCTCCTGTC 
CGAGACACCC GTGAGAGCCA GCTGGCACAC ATCAAGGACG AGCCTCCTCC ACTCTCCCCT 
GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC CTTGACCCAT TCTTCTCCCG GGAGCCCTCA 
TCTATGCACA TCTCCTCAAG CCTGCCCCCT GACACACAGA AGTTCCTTCG CTTTGCAGAG 
ACTCACCGCA CTGTGTTAAA CCAGATCCTA CGGCAGTCCA CGACCCACCT TGCTGATGGG 
CCTTTTGCTG TCCTGGTAGA CTACATTCGT GTCCTCGACT TTGATGTCAA GCGCAAATAT 
TTCCGCCAAG AGCTGGAGCG TTTAGATGAG GGGCTCCGGA AAGAAGACAT GGCTGTGCAT 
GTCCGTCGTG ACCATGTGTT TGAAGACTCC TATCGTGAGC TGCATCGCAA ATCCCCCGAA 
GAAATGAAGA ATCGATTGTA TATAGTATTT GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG 
CTCCTGCGGG AGTGGTATAT GATCATCTCT CGAGAGATGT TTAACCCTAT GTATGCCTTG 
TTCCGTACCT CACCTGGTGA TCGAGTCACC TACACCATCA ATCCATCTTC CCACTGCAAC 
CCCAACCACC TCAGCTACTT CAAGTTTGTC GGACGCATTG TGGCCAAAGC TGTATATGAC 
AACCGTCTTC TGGAGTGCTA CTTTACTCGA TCCTTTTACA AACACATCTT GGGCAAGTCA 
GTCAGATATA CAGATATGGA GAGTGAAGAT TACCACTTCT ACCAAGGTCT GGTTTATCTG 
CTGGAAAATG ATGTCTCCAC ACTAGGCTAT GACCTCACCT TCAGCACTGA GGTCCAAGAG 
TTTGGAGTTT GTGAAGTTCG TGACCTCAAA CCCAATGGGG CCAACATCTT GGTAACAGAG 
GAGAATAAGA AGGAGTATGT ACACCTGGTA TGCCAGATGA GAATGACAGG AGCCATCCGC 
AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC TATGAGATCA TTCCAAAGCG CCTCATTTCC 
ATCTTCACTG AGCAGGAGTT AGAGCTGCTT ATATCAGGAC TGCCCACCAT TGACATCGAT 
GATCTGAAAT CCAACACTGA ATACCACAAG TACCAGTCCA ACTCTATTCA GATCCAGTGG 
TTCTGGAGAG CATTGCGTTC TTTCGATCAA GCTGACCGTG CCAAGTTCCT CCAGTTTGTC 
ACAGGTACTT CCAAGGTACC CCTGCAAGGC TTTGCTGCCC TCGAAGGCAT GAATGGCATT 
CAGAAGTTTC AGATCCATCG AGATGACAGG TCCACAGATC GCCTGCCTTC AGCTCACACA 
TGTTTTAATC AGCTGGATCT GCCTGCCTAT GAGAGCTTTG AGAAGCTCCG CCACATGCTA 
CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_005261965.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005262022.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X18, mRNA.

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CloneID	OHu23451
Clone ID Related Accession (Same CDS sequence)	XM_005261965.3 , XM_005261965.4 , NM_031407.6 , NM_031407.7
Accession Version	XM_005261965.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005261965.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAATCAGC AAACAGTATC 
TTGTATAATG GCTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACGGA TAAGCAGCTT 
ATGGAGATTA AAGCAGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT 
CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCA 
GTGCTTGTAA GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATACCCTCAC 
CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC 
GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGATA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT 
GAGCATGAAG TAGATTTGTG CCGAAAAGAA TGTCCGTTTG TGATCAAGCC AAAGATCCAG 
AGACCCAATA CTACACAAGA AGGAGAGGAA ATGGAAACTG ATATGGATGG AGTCCAGTGT 
ATTCCACAAC GAGCAGCACT TCTGAAATCC ATGTTGAATT TCCTCAAGAA GGCCATCCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTCTGCC TACCTCCCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGCCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAACCA TCACTGCTCT CCTCACTCCA GGACAATGGA 
TTGACAGATG TCATGCTGCA TGCACTGCTT ATCAAAGATG TTCCTGCTAC CCGTGAAGTC 
CTTGGCTCCC TCCCAAATGT ATTCAGTGCA CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTTCAGT GTCAGCCTTT TGAACGCCTC TTCAAAGTTC TTCTGTCTCC AGATTACCTC 
CCAGCCATGC GGAGGAGGAG AAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTCG ATGAGCTCAT GAGACATCAG CCCACCCTTA AAACAGATGC AACGACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGGG ACCCCAAATA CATCTGTCAG 
AAGCCATCAA TCCAGAAGGC AGATGGCACT GCCACTGCTC CTCCCCCAAG GTCTAATCAT 
GCCGCAGAAG AAGCCTCTAG TGAGGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTA CCCAGCAAAA TGAAACTGAG CCTAATCAGC AGGTTGTTGG TACAGAGGAA 
CGTATTCCTA TTCCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
CTGAGCAACA ATACAACAGA TGACCACTGC CAGGAATTTG TGAATCAGAA AGGACTGTTG 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACAC TGTCACATGA ACCCAAAGTC 
CTTCAAGAGG GTCTCCTTCA GTTGGACTCC ATCCTCTCCT CCCTGGAGCC CTTACACCGC 
CCCATTGAAT CCCCTGGGGG CTCAGTGTTG TTGCGAGAAC TGGCTTGCGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC CCAGGCCACA CCTCTGCTGC ATGCACTCAC TGCTGCCCAT 
GCCTACATCA TGATGTTTGT TCATACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCCATC 
TCCGTAAACC AGTGGGGCTC TCAATTGGGT CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA 
TACTGTTCCC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TGTGTACCCC AAACAGCCTA 
CCATCTGGGT GTGAATTTGG CCAGGCAGAT ATGCAGAAAC TGGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGGGAAC AGGATGGAGC AGCTGGAAGT 
ATGGATGCTT CTACCCAGGG CTTATTAGAA GGCATTGGGC TAGATGGTGA CACATTGGCT 
CCCATGGAGA CAGATGAACC TACTGCTTCA GACTCTAAGG GCAAATCTAA AATCACACCA 
GCAATGGCTG CCAGAATTAA GCAAATCAAG CCTTTGTTAT CAGCTTCCTC CAGATTAGGC 
CGAGCACTTG CTGAGCTATT TGGACTTCTT GTTAAACTTT GTGTGGGATC TCCTGTCCGC 
CAGAGAAGGA GCCATCATGC TGCCAGCACC ACTACAGCAC CGACACCTGC CGCGCGATCA 
ACAGCCTCAG CTCTCACTAA GCTCTTGACT AAGGGGTTAT CTTGGCAGCC CCCACCATAT 
ACACCTACTC CCCGATTCAG GCTGACATTC TTCATCTGTT CAGTTGGTTT CACATCCCCA 
ATGCTGTTTG ATGAGAGGAA GTATCCCTAC CACCTCATGC TGCAAAAATT TCTCTGCTCC 
GGAGGCCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TGTCCATGGG AGGTAAAGTT 
CCTGTTTCTG AGGGATTGGA ACACTCAGAC TTGCCTGATG GCACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTGGTGGA GAAGATGGTG AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGCTGCCTG CCAAATTGCC TGGAGGTGTC CAGAACTTTC CCCAGTTCAG TGCACTGCGC 
TTCCTTGTGG TAACTCAGAA AGCAGCCTTT ACTTGCATCA AAAACTTATG GAACCGGAAA 
CCCCTGAAGG TATATGGTGG ACGAATGGCT GAATCGATGC TGGCCATTCT ATGCCACATC 
CTCCGAGGAG AACCTGTGAT TCGAGAGAGA CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA 
GAAGAGGATA CAGGGCAAGA GGAAGGTGGC TCCCGCCGGG AACCTCAAGT CAACCAGCAA 
CAACTGCAAC AGCTCATGGA CATGGGCTTC ACAAGGGAAC ATGCAATGGA GGCACTGTTG 
AACACCAGCA CCATGGAGCA GGCCACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATG 
GGAGGAGTTG TTCGGGATCT CAGCATGTCT GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA 
TGTCTAGAGA AGTTCCAGGA TGCTGACCCG TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACTATGT TGCCAGGCTG CTTCCACCTT CTTGATGAGC TGCCAGACAC AGTATACCGT 
GTGTGTGACC TGATCATGAC AGCAATCAAA CGTAATGGAG CAGATTATCG TGACATGATT 
CTGAAGCAAG TAGTCAATCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCCCTGACAA CAAGTGACAC AAAAACCGTG TCAGAGTGGA TAAGTCAGAT GGCCACACTG 
CCCCAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CGCTACTTTT TGAGGAGTTG 
AAGCTACCTT GTGCTTGGGT GGTTGAATCA AGTGGCATCC TTAATGTCCT AATCAAACTC 
TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA GCCAAGGAGC AGAAGGAAGT CCAGACCCCA 
AAGTGGATCA CACCAGTGTT GCTCCTGATT GATTTCTATG AAAAGACAGC CATCTCCTCA 
AAAAGGAGAG CCCAGATGAC TAAGTACCTG CAATCCAACA GCAACAACTG GCGCTGGTTT 
GATGATCGCT CTGGGCGTTG GTGTAGTTAC AGTGCAAGCA ACAATAGCAC TATTGATTCT 
GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA TTCACTGCAG GCCGAAGAAG ATACACGGTC 
CAATTCACTA CAATGGTGCA GGTTAATGAG GAAACAGGGA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTACCTCG GCTGAATAAA AATTCAAAAA ACAGCAATGG ACAGGAACTA 
GAGAAGACGC TGGAAGAAAG CAAAGAAATG GATATCAAAC GTAAAGAAAA TAAAGGCAAT 
GATACCCCTT TGGCCCTAGA GAGTACAAAC ACTGAAAAGG AGACAAGCCT GGAGGAAACA 
AAAATCGGGG AGATCCTGAT CCAGGGCTTG ACAGAAGATA TGGTGACTGT TTTAATCCGG 
GCCTGCGTGA GCATGCTGGG AGTCCCTGTG GACCCAGATA CTTTGCATGC CACCCTTCGT 
CTCTGTCTGA GGCTCACCCG GGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACCCGCATGA TCTTGAATTT GACCCAGAGC TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC 
ACCCTTCTCT TAAGACACAT CATTGAGGAC CCCTGTACCC TTCGTCATAC CATGGAAAAG 
GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT GGTAGCACTA CCTCTGGTGT TGTGTCTGGC 
AGCCTCGGCT CTCGGGAGAT CAACTACATC CTTCGTGTCC TTGGGCCAGC CGCATGCCGC 
AATCCAGACA TATTCACAGA AGTGGCCAAC TGCTGTATCC GCATCGCCCT TCCTGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGCCCTAAT 
GCTGTACAGC TGGTGAAGAC CACCCCTTTG AAGCCCTCAC CTCTGCCTGT CATCCCTGAT 
ACTATCAAGG AAGTGATCTA TGATATGCTG AATGCTCTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGATA AATCTGATCC TAAACCTGGG GTTATGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGATGATGT ATACCAGCAG TACCGGTCAC TTACGCGTCA GAGCAGTGAC 
TTTGATACGC AGTCAGGTTT TTCCATTAAT AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC 
ACTGAGACTT CCGCATCTGG GACCTCCCAA GGAGAGGCTT CAACTCCAGA GGAGTCTCGA 
GATGGGAAGA AAGATAAAGA AGGGGACCGG GCCTCTGAGG AAGGCAAACA GAAAGGCAAG 
GGCAGCAAAC CTTTAATGCC TACCTCCACT ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG 
TCCTATGTTG GTATTGCTAC CCTGATTGCC AACTACAGCT ACACTGTGGG CCAGTCTGAA 
CTGATCAAAG AGGACTGCAG TGTGCTAGCT TTTGTTCTGG ACCACCTGCT CCCACATACC 
CAGAATGCAG AAGACAAGGA CACCCCTGCC TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT 
GCTGCAGGGA GTGGCACAGA TGCCCAGGTG GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT 
GGACGGGCAC TGGCTATGGC TGAGAGTACA GAGAAACATG CCAGGCTTCA GGCAGTGATG 
TGTATCATCA GTACTATCAT GGAGTCCTGC CCCTCCACCT CCAGCTTCTA CAGCAGTGCC 
ACAGCGAAGA CCCAGCACAA TGGCATGAAC AACATCATTC GGCTTTTCCT GAAGAAGGGA 
CTGGTTAATG ACCTGGCCAG AGTACCTCAC AGCTTAGACC TGTCCAGTCC CAACATGGCC 
AACACAGTCA ATGCTGCTCT GAAGCCTTTG GAAACACTTT CCCGGATTGT GAACCAGCCC 
AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT AGCAAGAACA AGTCTGAGCA GGATGCCCAA 
GGAGCCTCTC AAGATTCCAG TAGCAACCAG CAGGACCCAG GCGAGCCTGG GGAAGCAGAA 
GTGCAGGAGG AGGATCATGA TGTCACTCAG ACAGAGGTGG CAGATGGGGA TATCATGGAT 
GGGGAGGCTG AAACCGACTC AGTGGTGATT GCTGGGCAGC CTGAGGTGCT CAGTTCACAA 
GAGATGCAGG TTGAGAATGA GCTGGAGGAC CTGATAGATG AGTTGCTTGA GAGGGATGGC 
GGATCTGGGA ACAGTACAAT TATAGTGAGC AGAAGTGGAG AGGATGAATC ACAAGAGGAC 
GTGCTGATGG ATGAAGCTCC TTCCAACCTC AGCCAAGCTT CCACCTTGCA GGCCAACCGA 
GAAGATTCCA TGAATATCCT GGACCCTGAG GATGAGGAGG AGCACACTCA GGAAGAGGAC 
AGCAGTGGCA GTAACGAGGA TGAGGATGAT AGTCAGGATG AAGAGGAGGA GGAGGAGGAA 
GATGAGGAAG ATGATCAGGA GGATGATGAA GGTGAAGAGG GAGATGAAGA CGATGACGAC 
GATGGCTCTG AGATGGAATT GGATGAGGAT TATCCTGATA TGAACGCTTC TCCCTTGGTC 
CGATTTGAGC GCTTTGACCG GGAGGATGAT CTCATCATTG AGTTTGACAA CATGTTCTCC 
AGTGCTACAG ACATCCCCCC ATCCCCAGGA AATATCCCTA CCACCCATCC ACTGATGGTG 
CGCCATGCAG ACCACAGTTC TCTGACACTG GGCAGTGGCT CTTCAACAAC TCGTCTCACC 
CAGGGCATCG GGCGCAGTCA GAGGACCCTA AGGCAGCTGA CGGCCAATAC TGGCCACACC 
ATTCATGTTC ACTACCCTGG GAATCGCCAG CCCAACCCTC CTCTTATACT GCAGAGGTTG 
CTTGGTCCCT CAGCTGCTGC TGACATCCTT CAGCTGAGCA GCAGCCTTCC CCTACAAAGC 
CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC GATGACGTCC ACATCATCGC CCGTTCTGAT 
GATGAGCTGC TGGATGACTT TTTCCATGAT CAGAGCACAG CTACCAGCCA AGCAGGAACC 
CTGTCCAGCA TCCCCACAGC CCTGACCCGC TGGACAGAAG AATGCAAAGT TCTCGATGCT 
GAGAGCATGC ATGACTGTGT TTCAGTGGTT AAAGTGTCCA TTGTCAATCA CCTGGAATTC 
CTGAGGGATG AGGAGCTGGA AGAAAGGCGA GAGAAGCGCA GGAAACAACT GGCTGAGGAA 
GAAACAAAGA TAACTGATAA AGGCAAAGAA GATAAGGAGA ACAGGGATCA GAGTGCACAG 
TGTACTGCAT CTAAGTCAAA TGACTCCACT GAACAGAATC TCTCAGATGG GACGCCTATG 
CCTGACAGCT ACCCAACAAC CCCATCTTCA ACTGATGCAG CTACATCTGA GTCCAAGGAG 
ACCCTTGGCA CTCTGCAATC CTCACAACAG CAACCAACAC TCCCAACCCC ACCAGCTTTG 
GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA GCTGGAGAAG GGGGCAGCTC TACACAGCTA 
TTGATGCCTG TAGAGCCAGA GGAATTGGGT CCCACAAGGC CAAGTGGGGA AGCAGAAACA 
ACTCAGATGG AGTTATCCCC AGCTCCCACT ATAACCTCAC TTTCCCCAGA GAGAGCTGAG 
GATTCTGATG CACTGACGGC TGTCAGCAGT CAGCTAGAAG GCTCTCCTAT GGATACAAGC 
AGCCTGGCTT CCTGTACCTT AGAGGAGGCT GTGGGTGACA CTTCAGCAGC TGGCAGTTCT 
GAGCAGCCCA GAGCAGGCAG CTCCACTCCT GGGGATGCCC CACCAGCTGT GGCGGAAGTG 
CAAGGCAGGA GTGATGGGTC AGGGGAATCT GCCCAGCCAC CTGAGGACAG CTCCCCACCT 
GCATCCTCTG AGAGCTCTTC CACCAGAGAT TCTGCCGTGG CCATTTCTGG AGCAGATTCC 
CGAGGAATCC TAGAAGAGCC GTTGCCTTCA ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG 
GGTATCAGTC TCCCTGAAGG TGTGGACCCC TCTTTTCTGG CTGCCCTGCC TGATGACATC 
CGTCGGGAAG TTCTACAGAA CCAGCTAGGC ATTCGTCCAC CAACCCGGAC TGCCCCCTCC 
ACAAATAGCT CAGCGCCTGC AGTGGTGGGG AATCCTGGTG TGACTGAAGT GAGCCCTGAG 
TTTCTGGCTG CCCTGCCTCC AGCCATTCAG GAGGAAGTAC TGGCACAGCA GAGAGCTGAG 
CAGCAGCGAC GAGAACTAGC ACAGAATGCC AGCTCAGACA CCCCTATGGA CCCTGTGACC 
TTCATCCAGA CTCTGCCCTC AGACCTGCGC CGTAGTGTCC TAGAGGATAT GGAGGACAGT 
GTGTTAGCTG TGATGCCACC TGACATTGCA GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA 
GAAGCCCGGC AGCGACAGCT CATGCATGAG CGTCTGTTTG GGCACAGTAG CACCTCCGCA 
CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC 
CAGTATACTC GCCTTGCTGT GCAGAGAGGT GGCACCTTCC AGATGGGGGG TAGCAGCAGC 
CATAACAGGC CTTCTGGCAG TAATGTAGAT ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT 
CTGGACCACG AAGCCCTTTC TTGTCTCTTG GTCCTACTTT TTGTGGATGA GCCAAAGCTC 
AATACTAGCC GTCTACACCG AGTACTGAGA AATCTCTGCT ACCATGCCCA GACCCGCCAC 
TGGGTCATCC GCAGTCTGCT CTCCATCTTG CAGCGCAGCA GTGAGAGTGA GCTATGCATT 
GAAACACCCA AACTCACTAC AAGTGAGGAA AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG 
TCAAGTAGCC ATGAGAACCG TCCCCTGGAC CTGCTACACA AGATGGAGTC AAAGAGCTCC 
AACCAGCTTT CCTGGCTCTC AGTATCCATG GATGCAGCCC TAGGCTGCAG GACTAATATA 
TTTCAGATCC AGCGTTCAGG GGGGCGTAAA CATACCGAGA AGCATGCAAG CGGTGGCTCC 
ACCGTCCACA TCCATCCCCA AGCTGCTCCT GTTGTCTGCA GACACGTTTT GGATACACTC 
ATTCAATTGG CCAAGGTATT TCCCAGCCAC TTCACACAGC AGCGGACCAA AGAAACAAAC 
TGTGAGAGTG ATCGGGAAAG GGGCAATAAG GCCTGTAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TTTGCACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTC 
AGCCGGAAAG GCAAGAACTC CGTGAAGTCA GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA 
ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAC 
CCAGTCATCC GCCGGAGCTC TCTCTTAACT GAGAAACTCC TCAGACTCCT TTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAGGTGTCA GAAGCACAGG CTAATTCTGG CAGCGGTGCT 
TCCTCCACCA CCACTGCCAC CTCAACCACA TCTACCACCA CCACCACTGC CGCCTCCACC 
ACGCCCACAC CCCCTACTGC ACCCACCCCT GTCACTTCTG CTCCAGCCCT GGTTGCTGCC 
ACGGCTATTT CCACCATTGT CGTAGCTGCT TCGACCACAG TGACTACCCC CACGACTGCT 
ACCACTACTG TTTCAATTTC TCCCACTACT AAGGGCAGCA AATCTCCAGC GAAGGTGAGT 
GATGGGGGCA GCAGCAGTAC AGACTTTAAG ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAGGAAGG CTTAGAGGAT 
GCAGCCAACG TACTACTGCA GCTCTCCCGG GGGGACTCTG GGACCCGGGA CACTGTTCTC 
AAGCTGCTAC TGAATGGAGC CCGCCATCTG GGTTATACCC TTTGTAAACA AATAGGTACC 
CTGCTGGCCG AGCTGCGGGA ATACAACCTC GAGCAGCAGC GGCGAGCCCA ATGTGAAACC 
CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG CCACAGACCA CCAAGCTGAA GGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAGAATGTG GTAATTGTGG CATCTCAGAA GCGACCTTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTCC GGGACGACAC GCGCCGGGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTAG CCAGTCAGAG 
GCGTCTGTCC GGAGGGAGGA ATCACCCATG GATGTGGACC AGCCATCTCC CAGTGCTCAA 
GATACTCAAT CCATTGCCTC CGATGGAACC CCACAGGGGG AGAAGGAAAA GGAAGAAAGA 
CCACCTGAGT TACCCCTGCT CAGCGAGCAG CTGAGTTTGG ACGAGCTGTG GGACATGCTT 
GGGGAGTGTC TAAAGGAACT AGAGGAATCC CATGACCAGC ATGCGGTGCT AGTGCTACAG 
CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT GCCACAGAGC GGGAGAGCAA GCCTCCTGTC 
CGAGACACCC GTGAGAGCCA GCTGGCACAC ATCAAGGACG AGCCTCCTCC ACTCTCCCCT 
GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC CTTGACCCAT TCTTCTCCCG GGAGCCCTCA 
TCTATGCACA TCTCCTCAAG CCTGCCCCCT GACACACAGA AGTTCCTTCG CTTTGCAGAG 
ACTCACCGCA CTGTGTTAAA CCAGATCCTA CGGCAGTCCA CGACCCACCT TGCTGATGGG 
CCTTTTGCTG TCCTGGTAGA CTACATTCGT GTCCTCGACT TTGATGTCAA GCGCAAATAT 
TTCCGCCAAG AGCTGGAGCG TTTAGATGAG GGGCTCCGGA AAGAAGACAT GGCTGTGCAT 
GTCCGTCGTG ACCATGTGTT TGAAGACTCC TATCGTGAGC TGCATCGCAA ATCCCCCGAA 
GAAATGAAGA ATCGATTGTA TATAGTATTT GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG 
CTCCTGCGGG AGTGGTATAT GATCATCTCT CGAGAGATGT TTAACCCTAT GTATGCCTTG 
TTCCGTACCT CACCTGGTGA TCGAGTCACC TACACCATCA ATCCATCTTC CCACTGCAAC 
CCCAACCACC TCAGCTACTT CAAGTTTGTC GGACGCATTG TGGCCAAAGC TGTATATGAC 
AACCGTCTTC TGGAGTGCTA CTTTACTCGA TCCTTTTACA AACACATCTT GGGCAAGTCA 
GTCAGATATA CAGATATGGA GAGTGAAGAT TACCACTTCT ACCAAGGTCT GGTTTATCTG 
CTGGAAAATG ATGTCTCCAC ACTAGGCTAT GACCTCACCT TCAGCACTGA GGTCCAAGAG 
TTTGGAGTTT GTGAAGTTCG TGACCTCAAA CCCAATGGGG CCAACATCTT GGTAACAGAG 
GAGAATAAGA AGGAGTATGT ACACCTGGTA TGCCAGATGA GAATGACAGG AGCCATCCGC 
AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC TATGAGATCA TTCCAAAGCG CCTCATTTCC 
ATCTTCACTG AGCAGGAGTT AGAGCTGCTT ATATCAGGAC TGCCCACCAT TGACATCGAT 
GATCTGAAAT CCAACACTGA ATACCACAAG TACCAGTCCA ACTCTATTCA GATCCAGTGG 
TTCTGGAGAG CATTGCGTTC TTTCGATCAA GCTGACCGTG CCAAGTTCCT CCAGTTTGTC 
ACAGGTACTT CCAAGGTACC CCTGCAAGGC TTTGCTGCCC TCGAAGGCAT GAATGGCATT 
CAGAAGTTTC AGATCCATCG AGATGACAGG TCCACAGATC GCCTGCCTTC AGCTCACACA 
TGTTTTAATC AGCTGGATCT GCCTGCCTAT GAGAGCTTTG AGAAGCTCCG CCACATGCTA 
CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005262022.1
CDS	433..13557
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_005261965.4
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005261965.4

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAATCAGC AAACAGTATC 
TTGTATAATG GCTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACGGA TAAGCAGCTT 
ATGGAGATTA AAGCAGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT 
CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCA 
GTGCTTGTAA GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATACCCTCAC 
CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC 
GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGATA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT 
GAGCATGAAG TAGATTTGTG CCGAAAAGAA TGTCCGTTTG TGATCAAGCC AAAGATCCAG 
AGACCCAATA CTACACAAGA AGGAGAGGAA ATGGAAACTG ATATGGATGG AGTCCAGTGT 
ATTCCACAAC GAGCAGCACT TCTGAAATCC ATGTTGAATT TCCTCAAGAA GGCCATCCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTCTGCC TACCTCCCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGCCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAACCA TCACTGCTCT CCTCACTCCA GGACAATGGA 
TTGACAGATG TCATGCTGCA TGCACTGCTT ATCAAAGATG TTCCTGCTAC CCGTGAAGTC 
CTTGGCTCCC TCCCAAATGT ATTCAGTGCA CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTTCAGT GTCAGCCTTT TGAACGCCTC TTCAAAGTTC TTCTGTCTCC AGATTACCTC 
CCAGCCATGC GGAGGAGGAG AAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTCG ATGAGCTCAT GAGACATCAG CCCACCCTTA AAACAGATGC AACGACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGGG ACCCCAAATA CATCTGTCAG 
AAGCCATCAA TCCAGAAGGC AGATGGCACT GCCACTGCTC CTCCCCCAAG GTCTAATCAT 
GCCGCAGAAG AAGCCTCTAG TGAGGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTA CCCAGCAAAA TGAAACTGAG CCTAATCAGC AGGTTGTTGG TACAGAGGAA 
CGTATTCCTA TTCCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
CTGAGCAACA ATACAACAGA TGACCACTGC CAGGAATTTG TGAATCAGAA AGGACTGTTG 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACAC TGTCACATGA ACCCAAAGTC 
CTTCAAGAGG GTCTCCTTCA GTTGGACTCC ATCCTCTCCT CCCTGGAGCC CTTACACCGC 
CCCATTGAAT CCCCTGGGGG CTCAGTGTTG TTGCGAGAAC TGGCTTGCGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC CCAGGCCACA CCTCTGCTGC ATGCACTCAC TGCTGCCCAT 
GCCTACATCA TGATGTTTGT TCATACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCCATC 
TCCGTAAACC AGTGGGGCTC TCAATTGGGT CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA 
TACTGTTCCC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TGTGTACCCC AAACAGCCTA 
CCATCTGGGT GTGAATTTGG CCAGGCAGAT ATGCAGAAAC TGGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGGGAAC AGGATGGAGC AGCTGGAAGT 
ATGGATGCTT CTACCCAGGG CTTATTAGAA GGCATTGGGC TAGATGGTGA CACATTGGCT 
CCCATGGAGA CAGATGAACC TACTGCTTCA GACTCTAAGG GCAAATCTAA AATCACACCA 
GCAATGGCTG CCAGAATTAA GCAAATCAAG CCTTTGTTAT CAGCTTCCTC CAGATTAGGC 
CGAGCACTTG CTGAGCTATT TGGACTTCTT GTTAAACTTT GTGTGGGATC TCCTGTCCGC 
CAGAGAAGGA GCCATCATGC TGCCAGCACC ACTACAGCAC CGACACCTGC CGCGCGATCA 
ACAGCCTCAG CTCTCACTAA GCTCTTGACT AAGGGGTTAT CTTGGCAGCC CCCACCATAT 
ACACCTACTC CCCGATTCAG GCTGACATTC TTCATCTGTT CAGTTGGTTT CACATCCCCA 
ATGCTGTTTG ATGAGAGGAA GTATCCCTAC CACCTCATGC TGCAAAAATT TCTCTGCTCC 
GGAGGCCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TGTCCATGGG AGGTAAAGTT 
CCTGTTTCTG AGGGATTGGA ACACTCAGAC TTGCCTGATG GCACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTGGTGGA GAAGATGGTG AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGCTGCCTG CCAAATTGCC TGGAGGTGTC CAGAACTTTC CCCAGTTCAG TGCACTGCGC 
TTCCTTGTGG TAACTCAGAA AGCAGCCTTT ACTTGCATCA AAAACTTATG GAACCGGAAA 
CCCCTGAAGG TATATGGTGG ACGAATGGCT GAATCGATGC TGGCCATTCT ATGCCACATC 
CTCCGAGGAG AACCTGTGAT TCGAGAGAGA CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA 
GAAGAGGATA CAGGGCAAGA GGAAGGTGGC TCCCGCCGGG AACCTCAAGT CAACCAGCAA 
CAACTGCAAC AGCTCATGGA CATGGGCTTC ACAAGGGAAC ATGCAATGGA GGCACTGTTG 
AACACCAGCA CCATGGAGCA GGCCACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATG 
GGAGGAGTTG TTCGGGATCT CAGCATGTCT GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA 
TGTCTAGAGA AGTTCCAGGA TGCTGACCCG TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACTATGT TGCCAGGCTG CTTCCACCTT CTTGATGAGC TGCCAGACAC AGTATACCGT 
GTGTGTGACC TGATCATGAC AGCAATCAAA CGTAATGGAG CAGATTATCG TGACATGATT 
CTGAAGCAAG TAGTCAATCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCCCTGACAA CAAGTGACAC AAAAACCGTG TCAGAGTGGA TAAGTCAGAT GGCCACACTG 
CCCCAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CGCTACTTTT TGAGGAGTTG 
AAGCTACCTT GTGCTTGGGT GGTTGAATCA AGTGGCATCC TTAATGTCCT AATCAAACTC 
TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA GCCAAGGAGC AGAAGGAAGT CCAGACCCCA 
AAGTGGATCA CACCAGTGTT GCTCCTGATT GATTTCTATG AAAAGACAGC CATCTCCTCA 
AAAAGGAGAG CCCAGATGAC TAAGTACCTG CAATCCAACA GCAACAACTG GCGCTGGTTT 
GATGATCGCT CTGGGCGTTG GTGTAGTTAC AGTGCAAGCA ACAATAGCAC TATTGATTCT 
GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA TTCACTGCAG GCCGAAGAAG ATACACGGTC 
CAATTCACTA CAATGGTGCA GGTTAATGAG GAAACAGGGA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTACCTCG GCTGAATAAA AATTCAAAAA ACAGCAATGG ACAGGAACTA 
GAGAAGACGC TGGAAGAAAG CAAAGAAATG GATATCAAAC GTAAAGAAAA TAAAGGCAAT 
GATACCCCTT TGGCCCTAGA GAGTACAAAC ACTGAAAAGG AGACAAGCCT GGAGGAAACA 
AAAATCGGGG AGATCCTGAT CCAGGGCTTG ACAGAAGATA TGGTGACTGT TTTAATCCGG 
GCCTGCGTGA GCATGCTGGG AGTCCCTGTG GACCCAGATA CTTTGCATGC CACCCTTCGT 
CTCTGTCTGA GGCTCACCCG GGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACCCGCATGA TCTTGAATTT GACCCAGAGC TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC 
ACCCTTCTCT TAAGACACAT CATTGAGGAC CCCTGTACCC TTCGTCATAC CATGGAAAAG 
GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT GGTAGCACTA CCTCTGGTGT TGTGTCTGGC 
AGCCTCGGCT CTCGGGAGAT CAACTACATC CTTCGTGTCC TTGGGCCAGC CGCATGCCGC 
AATCCAGACA TATTCACAGA AGTGGCCAAC TGCTGTATCC GCATCGCCCT TCCTGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGCCCTAAT 
GCTGTACAGC TGGTGAAGAC CACCCCTTTG AAGCCCTCAC CTCTGCCTGT CATCCCTGAT 
ACTATCAAGG AAGTGATCTA TGATATGCTG AATGCTCTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGATA AATCTGATCC TAAACCTGGG GTTATGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGATGATGT ATACCAGCAG TACCGGTCAC TTACGCGTCA GAGCAGTGAC 
TTTGATACGC AGTCAGGTTT TTCCATTAAT AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC 
ACTGAGACTT CCGCATCTGG GACCTCCCAA GGAGAGGCTT CAACTCCAGA GGAGTCTCGA 
GATGGGAAGA AAGATAAAGA AGGGGACCGG GCCTCTGAGG AAGGCAAACA GAAAGGCAAG 
GGCAGCAAAC CTTTAATGCC TACCTCCACT ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG 
TCCTATGTTG GTATTGCTAC CCTGATTGCC AACTACAGCT ACACTGTGGG CCAGTCTGAA 
CTGATCAAAG AGGACTGCAG TGTGCTAGCT TTTGTTCTGG ACCACCTGCT CCCACATACC 
CAGAATGCAG AAGACAAGGA CACCCCTGCC TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT 
GCTGCAGGGA GTGGCACAGA TGCCCAGGTG GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT 
GGACGGGCAC TGGCTATGGC TGAGAGTACA GAGAAACATG CCAGGCTTCA GGCAGTGATG 
TGTATCATCA GTACTATCAT GGAGTCCTGC CCCTCCACCT CCAGCTTCTA CAGCAGTGCC 
ACAGCGAAGA CCCAGCACAA TGGCATGAAC AACATCATTC GGCTTTTCCT GAAGAAGGGA 
CTGGTTAATG ACCTGGCCAG AGTACCTCAC AGCTTAGACC TGTCCAGTCC CAACATGGCC 
AACACAGTCA ATGCTGCTCT GAAGCCTTTG GAAACACTTT CCCGGATTGT GAACCAGCCC 
AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT AGCAAGAACA AGTCTGAGCA GGATGCCCAA 
GGAGCCTCTC AAGATTCCAG TAGCAACCAG CAGGACCCAG GCGAGCCTGG GGAAGCAGAA 
GTGCAGGAGG AGGATCATGA TGTCACTCAG ACAGAGGTGG CAGATGGGGA TATCATGGAT 
GGGGAGGCTG AAACCGACTC AGTGGTGATT GCTGGGCAGC CTGAGGTGCT CAGTTCACAA 
GAGATGCAGG TTGAGAATGA GCTGGAGGAC CTGATAGATG AGTTGCTTGA GAGGGATGGC 
GGATCTGGGA ACAGTACAAT TATAGTGAGC AGAAGTGGAG AGGATGAATC ACAAGAGGAC 
GTGCTGATGG ATGAAGCTCC TTCCAACCTC AGCCAAGCTT CCACCTTGCA GGCCAACCGA 
GAAGATTCCA TGAATATCCT GGACCCTGAG GATGAGGAGG AGCACACTCA GGAAGAGGAC 
AGCAGTGGCA GTAACGAGGA TGAGGATGAT AGTCAGGATG AAGAGGAGGA GGAGGAGGAA 
GATGAGGAAG ATGATCAGGA GGATGATGAA GGTGAAGAGG GAGATGAAGA CGATGACGAC 
GATGGCTCTG AGATGGAATT GGATGAGGAT TATCCTGATA TGAACGCTTC TCCCTTGGTC 
CGATTTGAGC GCTTTGACCG GGAGGATGAT CTCATCATTG AGTTTGACAA CATGTTCTCC 
AGTGCTACAG ACATCCCCCC ATCCCCAGGA AATATCCCTA CCACCCATCC ACTGATGGTG 
CGCCATGCAG ACCACAGTTC TCTGACACTG GGCAGTGGCT CTTCAACAAC TCGTCTCACC 
CAGGGCATCG GGCGCAGTCA GAGGACCCTA AGGCAGCTGA CGGCCAATAC TGGCCACACC 
ATTCATGTTC ACTACCCTGG GAATCGCCAG CCCAACCCTC CTCTTATACT GCAGAGGTTG 
CTTGGTCCCT CAGCTGCTGC TGACATCCTT CAGCTGAGCA GCAGCCTTCC CCTACAAAGC 
CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC GATGACGTCC ACATCATCGC CCGTTCTGAT 
GATGAGCTGC TGGATGACTT TTTCCATGAT CAGAGCACAG CTACCAGCCA AGCAGGAACC 
CTGTCCAGCA TCCCCACAGC CCTGACCCGC TGGACAGAAG AATGCAAAGT TCTCGATGCT 
GAGAGCATGC ATGACTGTGT TTCAGTGGTT AAAGTGTCCA TTGTCAATCA CCTGGAATTC 
CTGAGGGATG AGGAGCTGGA AGAAAGGCGA GAGAAGCGCA GGAAACAACT GGCTGAGGAA 
GAAACAAAGA TAACTGATAA AGGCAAAGAA GATAAGGAGA ACAGGGATCA GAGTGCACAG 
TGTACTGCAT CTAAGTCAAA TGACTCCACT GAACAGAATC TCTCAGATGG GACGCCTATG 
CCTGACAGCT ACCCAACAAC CCCATCTTCA ACTGATGCAG CTACATCTGA GTCCAAGGAG 
ACCCTTGGCA CTCTGCAATC CTCACAACAG CAACCAACAC TCCCAACCCC ACCAGCTTTG 
GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA GCTGGAGAAG GGGGCAGCTC TACACAGCTA 
TTGATGCCTG TAGAGCCAGA GGAATTGGGT CCCACAAGGC CAAGTGGGGA AGCAGAAACA 
ACTCAGATGG AGTTATCCCC AGCTCCCACT ATAACCTCAC TTTCCCCAGA GAGAGCTGAG 
GATTCTGATG CACTGACGGC TGTCAGCAGT CAGCTAGAAG GCTCTCCTAT GGATACAAGC 
AGCCTGGCTT CCTGTACCTT AGAGGAGGCT GTGGGTGACA CTTCAGCAGC TGGCAGTTCT 
GAGCAGCCCA GAGCAGGCAG CTCCACTCCT GGGGATGCCC CACCAGCTGT GGCGGAAGTG 
CAAGGCAGGA GTGATGGGTC AGGGGAATCT GCCCAGCCAC CTGAGGACAG CTCCCCACCT 
GCATCCTCTG AGAGCTCTTC CACCAGAGAT TCTGCCGTGG CCATTTCTGG AGCAGATTCC 
CGAGGAATCC TAGAAGAGCC GTTGCCTTCA ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG 
GGTATCAGTC TCCCTGAAGG TGTGGACCCC TCTTTTCTGG CTGCCCTGCC TGATGACATC 
CGTCGGGAAG TTCTACAGAA CCAGCTAGGC ATTCGTCCAC CAACCCGGAC TGCCCCCTCC 
ACAAATAGCT CAGCGCCTGC AGTGGTGGGG AATCCTGGTG TGACTGAAGT GAGCCCTGAG 
TTTCTGGCTG CCCTGCCTCC AGCCATTCAG GAGGAAGTAC TGGCACAGCA GAGAGCTGAG 
CAGCAGCGAC GAGAACTAGC ACAGAATGCC AGCTCAGACA CCCCTATGGA CCCTGTGACC 
TTCATCCAGA CTCTGCCCTC AGACCTGCGC CGTAGTGTCC TAGAGGATAT GGAGGACAGT 
GTGTTAGCTG TGATGCCACC TGACATTGCA GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA 
GAAGCCCGGC AGCGACAGCT CATGCATGAG CGTCTGTTTG GGCACAGTAG CACCTCCGCA 
CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC 
CAGTATACTC GCCTTGCTGT GCAGAGAGGT GGCACCTTCC AGATGGGGGG TAGCAGCAGC 
CATAACAGGC CTTCTGGCAG TAATGTAGAT ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT 
CTGGACCACG AAGCCCTTTC TTGTCTCTTG GTCCTACTTT TTGTGGATGA GCCAAAGCTC 
AATACTAGCC GTCTACACCG AGTACTGAGA AATCTCTGCT ACCATGCCCA GACCCGCCAC 
TGGGTCATCC GCAGTCTGCT CTCCATCTTG CAGCGCAGCA GTGAGAGTGA GCTATGCATT 
GAAACACCCA AACTCACTAC AAGTGAGGAA AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG 
TCAAGTAGCC ATGAGAACCG TCCCCTGGAC CTGCTACACA AGATGGAGTC AAAGAGCTCC 
AACCAGCTTT CCTGGCTCTC AGTATCCATG GATGCAGCCC TAGGCTGCAG GACTAATATA 
TTTCAGATCC AGCGTTCAGG GGGGCGTAAA CATACCGAGA AGCATGCAAG CGGTGGCTCC 
ACCGTCCACA TCCATCCCCA AGCTGCTCCT GTTGTCTGCA GACACGTTTT GGATACACTC 
ATTCAATTGG CCAAGGTATT TCCCAGCCAC TTCACACAGC AGCGGACCAA AGAAACAAAC 
TGTGAGAGTG ATCGGGAAAG GGGCAATAAG GCCTGTAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TTTGCACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTC 
AGCCGGAAAG GCAAGAACTC CGTGAAGTCA GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA 
ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAC 
CCAGTCATCC GCCGGAGCTC TCTCTTAACT GAGAAACTCC TCAGACTCCT TTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAGGTGTCA GAAGCACAGG CTAATTCTGG CAGCGGTGCT 
TCCTCCACCA CCACTGCCAC CTCAACCACA TCTACCACCA CCACCACTGC CGCCTCCACC 
ACGCCCACAC CCCCTACTGC ACCCACCCCT GTCACTTCTG CTCCAGCCCT GGTTGCTGCC 
ACGGCTATTT CCACCATTGT CGTAGCTGCT TCGACCACAG TGACTACCCC CACGACTGCT 
ACCACTACTG TTTCAATTTC TCCCACTACT AAGGGCAGCA AATCTCCAGC GAAGGTGAGT 
GATGGGGGCA GCAGCAGTAC AGACTTTAAG ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAGGAAGG CTTAGAGGAT 
GCAGCCAACG TACTACTGCA GCTCTCCCGG GGGGACTCTG GGACCCGGGA CACTGTTCTC 
AAGCTGCTAC TGAATGGAGC CCGCCATCTG GGTTATACCC TTTGTAAACA AATAGGTACC 
CTGCTGGCCG AGCTGCGGGA ATACAACCTC GAGCAGCAGC GGCGAGCCCA ATGTGAAACC 
CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG CCACAGACCA CCAAGCTGAA GGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAGAATGTG GTAATTGTGG CATCTCAGAA GCGACCTTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTCC GGGACGACAC GCGCCGGGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTAG CCAGTCAGAG 
GCGTCTGTCC GGAGGGAGGA ATCACCCATG GATGTGGACC AGCCATCTCC CAGTGCTCAA 
GATACTCAAT CCATTGCCTC CGATGGAACC CCACAGGGGG AGAAGGAAAA GGAAGAAAGA 
CCACCTGAGT TACCCCTGCT CAGCGAGCAG CTGAGTTTGG ACGAGCTGTG GGACATGCTT 
GGGGAGTGTC TAAAGGAACT AGAGGAATCC CATGACCAGC ATGCGGTGCT AGTGCTACAG 
CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT GCCACAGAGC GGGAGAGCAA GCCTCCTGTC 
CGAGACACCC GTGAGAGCCA GCTGGCACAC ATCAAGGACG AGCCTCCTCC ACTCTCCCCT 
GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC CTTGACCCAT TCTTCTCCCG GGAGCCCTCA 
TCTATGCACA TCTCCTCAAG CCTGCCCCCT GACACACAGA AGTTCCTTCG CTTTGCAGAG 
ACTCACCGCA CTGTGTTAAA CCAGATCCTA CGGCAGTCCA CGACCCACCT TGCTGATGGG 
CCTTTTGCTG TCCTGGTAGA CTACATTCGT GTCCTCGACT TTGATGTCAA GCGCAAATAT 
TTCCGCCAAG AGCTGGAGCG TTTAGATGAG GGGCTCCGGA AAGAAGACAT GGCTGTGCAT 
GTCCGTCGTG ACCATGTGTT TGAAGACTCC TATCGTGAGC TGCATCGCAA ATCCCCCGAA 
GAAATGAAGA ATCGATTGTA TATAGTATTT GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG 
CTCCTGCGGG AGTGGTATAT GATCATCTCT CGAGAGATGT TTAACCCTAT GTATGCCTTG 
TTCCGTACCT CACCTGGTGA TCGAGTCACC TACACCATCA ATCCATCTTC CCACTGCAAC 
CCCAACCACC TCAGCTACTT CAAGTTTGTC GGACGCATTG TGGCCAAAGC TGTATATGAC 
AACCGTCTTC TGGAGTGCTA CTTTACTCGA TCCTTTTACA AACACATCTT GGGCAAGTCA 
GTCAGATATA CAGATATGGA GAGTGAAGAT TACCACTTCT ACCAAGGTCT GGTTTATCTG 
CTGGAAAATG ATGTCTCCAC ACTAGGCTAT GACCTCACCT TCAGCACTGA GGTCCAAGAG 
TTTGGAGTTT GTGAAGTTCG TGACCTCAAA CCCAATGGGG CCAACATCTT GGTAACAGAG 
GAGAATAAGA AGGAGTATGT ACACCTGGTA TGCCAGATGA GAATGACAGG AGCCATCCGC 
AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC TATGAGATCA TTCCAAAGCG CCTCATTTCC 
ATCTTCACTG AGCAGGAGTT AGAGCTGCTT ATATCAGGAC TGCCCACCAT TGACATCGAT 
GATCTGAAAT CCAACACTGA ATACCACAAG TACCAGTCCA ACTCTATTCA GATCCAGTGG 
TTCTGGAGAG CATTGCGTTC TTTCGATCAA GCTGACCGTG CCAAGTTCCT CCAGTTTGTC 
ACAGGTACTT CCAAGGTACC CCTGCAAGGC TTTGCTGCCC TCGAAGGCAT GAATGGCATT 
CAGAAGTTTC AGATCCATCG AGATGACAGG TCCACAGATC GCCTGCCTTC AGCTCACACA 
TGTTTTAATC AGCTGGATCT GCCTGCCTAT GAGAGCTTTG AGAAGCTCCG CCACATGCTA 
CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_011530747.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529049.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X14, mRNA.

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CloneID	OHu63653
Clone ID Related Accession (Same CDS sequence)	XM_011530747.1 , XM_024452322.1 , XM_011530751.2
Accession Version	XM_011530747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAATCAGC AAACAGTATC 
TTGTATAATG GCTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACGGA TAAGCAGCTT 
ATGGAGATTA AAGCAGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT 
CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCA 
GTGCTTGTAA GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATACCCTCAC 
CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC 
GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGATA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT 
GAGCATGAAG TAGATTTGTG CCGAAAAGAA TGTCCGTTTG TGATCAAGCC AAAGATCCAG 
AGACCCAATA CTACACAAGA AGGAGAGGAA ATGGAAACTG ATATGGATGT TGCAGATGTG 
GCTATGGAAA GCAGTCCAGG CTCATCCATC TCTATGGAGC ACAGGCTGGA TGTTGAATTA 
AGGGCATCAG GTTCCAGCAG CAGCACTAAC ATCTCTTCTG GCCCCAGCCC TGGTCCCAGT 
CCCGGCCCCG GCACCGGCCC TGGCCCCGGC CCCGGCCCCG GCCCCGGCCC TGGCCCCGGC 
CCCGGCCCCG GTCCTGGTCC CGGCCCTGGC CCCGGCCCTG GCCCTGGCCC CCGTCCTGGA 
GTCCAGTGTA TTCCACAACG AGCAGCACTT CTGAAATCCA TGTTGAATTT CCTCAAGAAG 
GCCATCCAAG ACCCTGCTTT CTCAGATGGC ATACGACATG TGATGGATGG TTCTCTGCCT 
ACCTCCCTGA AACACATCAT CAGCAATGCA GAATACTATG GCCCATCACT CTTCCTCCTA 
GCTACTGAAG TGGTGACTGT GTTTGTATTT CAAGAACCAT CACTGCTCTC CTCACTCCAG 
GACAATGGAT TGACAGATGT CATGCTGCAT GCACTGCTTA TCAAAGATGT TCCTGCTACC 
CGTGAAGTCC TTGGCTCCCT CCCAAATGTA TTCAGTGCAC TCTGTTTGAA TGCCCGAGGT 
CTTCAGTCTT TTGTTCAGTG TCAGCCTTTT GAACGCCTCT TCAAAGTTCT TCTGTCTCCA 
GATTACCTCC CAGCCATGCG GAGGAGGAGA AGTTCTGATC CCCTTGGGGA TACTGCATCC 
AACCTGGGGA GTGCTGTCGA TGAGCTCATG AGACATCAGC CCACCCTTAA AACAGATGCA 
ACGACTGCCA TCATCAAGTT ACTTGAAGAA ATCTGTAATC TTGGAAGGGA CCCCAAATAC 
ATCTGTCAGA AGCCATCAAT CCAGAAGGCA GATGGCACTG CCACTGCTCC TCCCCCAAGG 
TCTAATCATG CCGCAGAAGA AGCCTCTAGT GAGGATGAGG AGGAAGAGGA AGTACAGGCC 
ATGCAGAGCT TTAATTCTAC CCAGCAAAAT GAAACTGAGC CTAATCAGCA GGTTGTTGGT 
ACAGAGGAAC GTATTCCTAT TCCCCTCATG GATTACATCC TTAATGTGAT GAAATTTGTG 
GAATCTATTC TGAGCAACAA TACAACAGAT GACCACTGCC AGGAATTTGT GAATCAGAAA 
GGACTGTTGC CTTTGGTTAC CATTTTGGGT CTTCCCAATC TGCCCATTGA CTTTCCCACA 
TCTGCTGCCT GTCAGGCTGT TGCAGGTGTC TGCAAATCCA TATTGACACT GTCACATGAA 
CCCAAAGTCC TTCAAGAGGG TCTCCTTCAG TTGGACTCCA TCCTCTCCTC CCTGGAGCCC 
TTACACCGCC CCATTGAATC CCCTGGGGGC TCAGTGTTGT TGCGAGAACT GGCTTGCGCA 
GGCAATGTTG CTGATGCTAC CCTCTCAGCC CAGGCCACAC CTCTGCTGCA TGCACTCACT 
GCTGCCCATG CCTACATCAT GATGTTTGTT CATACTTGCA GAGTTGGACA GAGTGAAATT 
CGTTCCATCT CCGTAAACCA GTGGGGCTCT CAATTGGGTC TGAGTGTTTT GAGCAAGCTG 
AGCCAGTTAT ACTGTTCCCT GGTGTGGGAA AGCACTGTCC TCCTCTCTCT GTGTACCCCA 
AACAGCCTAC CATCTGGGTG TGAATTTGGC CAGGCAGATA TGCAGAAACT GGTTCCAAAG 
GATGAGAAGG CAGGTACGAC CCAGGGCGGA AAAAGATCAG ATGGGGAACA GGATGGAGCA 
GCTGGAAGTA TGGATGCTTC TACCCAGGGC TTATTAGAAG GCATTGGGCT AGATGGTGAC 
ACATTGGCTC CCATGGAGAC AGATGAACCT ACTGCTTCAG ACTCTAAGGG CAAATCTAAA 
ATCACACCAG CAATGGCTGC CAGAATTAAG CAAATCAAGC CTTTGTTATC AGCTTCCTCC 
AGATTAGGCC GAGCACTTGC TGAGCTATTT GGACTTCTTG TTAAACTTTG TGTGGGATCT 
CCTGTCCGCC AGAGAAGGAG CCATCATGCT GCCAGCACCA CTACAGCACC GACACCTGCC 
GCGCGATCAA CAGCCTCAGC TCTCACTAAG CTCTTGACTA AGGGGTTATC TTGGCAGCCC 
CCACCATATA CACCTACTCC CCGATTCAGG CTGACATTCT TCATCTGTTC AGTTGGTTTC 
ACATCCCCAA TGCTGTTTGA TGAGAGGAAG TATCCCTACC ACCTCATGCT GCAAAAATTT 
CTCTGCTCCG GAGGCCACAA TGCTCTTTTT GAAACTTTCA ACTGGGCTCT GTCCATGGGA 
GGTAAAGTTC CTGTTTCTGA GGGATTGGAA CACTCAGACT TGCCTGATGG CACAGGAGAA 
TTCCTAGATG CCTGGCTTAT GCTGGTGGAG AAGATGGTGA ATCCCACCAC GGTGCTTGAA 
TCTCCACATT CGCTGCCTGC CAAATTGCCT GGAGGTGTCC AGAACTTTCC CCAGTTCAGT 
GCACTGCGCT TCCTTGTGGT AACTCAGAAA GCAGCCTTTA CTTGCATCAA AAACTTATGG 
AACCGGAAAC CCCTGAAGGT ATATGGTGGA CGAATGGCTG AATCGATGCT GGCCATTCTA 
TGCCACATCC TCCGAGGAGA ACCTGTGATT CGAGAGAGAC TAAGCAAGGA GAAGGAGGGG 
TCTCGAGGAG AAGAGGATAC AGGGCAAGAG GAAGGTGGCT CCCGCCGGGA ACCTCAAGTC 
AACCAGCAAC AACTGCAACA GCTCATGGAC ATGGGCTTCA CAAGGGAACA TGCAATGGAG 
GCACTGTTGA ACACCAGCAC CATGGAGCAG GCCACAGAGT ACCTTTTAAC CCACCCTCCT 
CCAATCATGG GAGGAGTTGT TCGGGATCTC AGCATGTCTG AAGAGGACCA GATGATGAGA 
GCAATTGCTA TGTCTCTGGG ACAGGATATT CCAATGGATC AAAGGGCAGA GTCACCTGAG 
GAAGTTGCTT GCCGGAAGGA GGAAGAGGAA CGGAAAGCTC GGGAAAAGCA GGAGGAGGAA 
GAGGCTAAAT GTCTAGAGAA GTTCCAGGAT GCTGACCCGT TGGAACAAGA TGAGCTCCAC 
ACTTTCACAG ATACTATGTT GCCAGGCTGC TTCCACCTTC TTGATGAGCT GCCAGACACA 
GTATACCGTG TGTGTGACCT GATCATGACA GCAATCAAAC GTAATGGAGC AGATTATCGT 
GACATGATTC TGAAGCAAGT AGTCAATCAG GTGTGGGAAG CTGCTGATGT ATTGATCAAA 
GCTGCTCTTC CCCTGACAAC AAGTGACACA AAAACCGTGT CAGAGTGGAT AAGTCAGATG 
GCCACACTGC CCCAGGCCTC CAATTTGGCT ACTAGAATCT TGCTTTTAAC GCTACTTTTT 
GAGGAGTTGA AGCTACCTTG TGCTTGGGTG GTTGAATCAA GTGGCATCCT TAATGTCCTA 
ATCAAACTCT TGGAAGTGGT TCAGCCCTGC CTCCAGGCAG CCAAGGAGCA GAAGGAAGTC 
CAGACCCCAA AGTGGATCAC ACCAGTGTTG CTCCTGATTG ATTTCTATGA AAAGACAGCC 
ATCTCCTCAA AAAGGAGAGC CCAGATGACT AAGTACCTGC AATCCAACAG CAACAACTGG 
CGCTGGTTTG ATGATCGCTC TGGGCGTTGG TGTAGTTACA GTGCAAGCAA CAATAGCACT 
ATTGATTCTG CCTGGAAATC TGGAGAGACA AGCGTGCGAT TCACTGCAGG CCGAAGAAGA 
TACACGGTCC AATTCACTAC AATGGTGCAG GTTAATGAGG AAACAGGGAA CCGACGCCCT 
GTGATGCTGA CTCTCCTCAG GGTACCTCGG CTGAATAAAA ATTCAAAAAA CAGCAATGGA 
CAGGAACTAG AGAAGACGCT GGAAGAAAGC AAAGAAATGG ATATCAAACG TAAAGAAAAT 
AAAGGCAATG ATACCCCTTT GGCCCTAGAG AGTACAAACA CTGAAAAGGA GACAAGCCTG 
GAGGAAACAA AAATCGGGGA GATCCTGATC CAGGGCTTGA CAGAAGATAT GGTGACTGTT 
TTAATCCGGG CCTGCGTGAG CATGCTGGGA GTCCCTGTGG ACCCAGATAC TTTGCATGCC 
ACCCTTCGTC TCTGTCTGAG GCTCACCCGG GACCACAAAT ATGCCATGAT GTTTGCAGAA 
CTGAAGAGTA CCCGCATGAT CTTGAATTTG ACCCAGAGCT CAGGCTTCAA TGGGTTTACT 
CCCCTGGTCA CCCTTCTCTT AAGACACATC ATTGAGGACC CCTGTACCCT TCGTCATACC 
ATGGAAAAGG TTGTTCGCTC AGCAGCTACA AGTGGAGCTG GTAGCACTAC CTCTGGTGTT 
GTGTCTGGCA GCCTCGGCTC TCGGGAGATC AACTACATCC TTCGTGTCCT TGGGCCAGCC 
GCATGCCGCA ATCCAGACAT ATTCACAGAA GTGGCCAACT GCTGTATCCG CATCGCCCTT 
CCTGCCCCTC GAGGCTCAGG AACTGCTTCA GATGATGAAT TTGAGAATCT TAGAATTAAA 
GGCCCTAATG CTGTACAGCT GGTGAAGACC ACCCCTTTGA AGCCCTCACC TCTGCCTGTC 
ATCCCTGATA CTATCAAGGA AGTGATCTAT GATATGCTGA ATGCTCTGGC TGCATACCAT 
GCTCCAGAGG AAGCAGATAA ATCTGATCCT AAACCTGGGG TTATGACCCA AGAGGTTGGC 
CAGCTCCTGC AAGACATGGG TGATGATGTA TACCAGCAGT ACCGGTCACT TACGCGTCAG 
AGCAGTGACT TTGATACGCA GTCAGGTTTT TCCATTAATA GTCAGGTCTT TGCTGCAGAT 
GGTGCCTCCA CTGAGACTTC CGCATCTGGG ACCTCCCAAG GAGAGGCTTC AACTCCAGAG 
GAGTCTCGAG ATGGGAAGAA AGATAAAGAA GGGGACCGGG CCTCTGAGGA AGGCAAACAG 
AAAGGCAAGG GCAGCAAACC TTTAATGCCT ACCTCCACTA TCCTTCGTCT TCTGGCAGAG 
TTGGTGAGGT CCTATGTTGG TATTGCTACC CTGATTGCCA ACTACAGCTA CACTGTGGGC 
CAGTCTGAAC TGATCAAAGA GGACTGCAGT GTGCTAGCTT TTGTTCTGGA CCACCTGCTC 
CCACATACCC AGAATGCAGA AGACAAGGAC ACCCCTGCCT TGGCCCGCCT GTTCCTCGCA 
AGCCTGGCTG CTGCAGGGAG TGGCACAGAT GCCCAGGTGG CCCTAGTGAA TGAAGTAAAA 
GCAGCCCTTG GACGGGCACT GGCTATGGCT GAGAGTACAG AGAAACATGC CAGGCTTCAG 
GCAGTGATGT GTATCATCAG TACTATCATG GAGTCCTGCC CCTCCACCTC CAGCTTCTAC 
AGCAGTGCCA CAGCGAAGAC CCAGCACAAT GGCATGAACA ACATCATTCG GCTTTTCCTG 
AAGAAGGGAC TGGTTAATGA CCTGGCCAGA GTACCTCACA GCTTAGACCT GTCCAGTCCC 
AACATGGCCA ACACAGTCAA TGCTGCTCTG AAGCCTTTGG AAACACTTTC CCGGATTGTG 
AACCAGCCCA GTAGCCTTTT TGGCAGCAAG AGTGCTTCTA GCAAGAACAA GTCTGAGCAG 
GATGCCCAAG GAGCCTCTCA AGATTCCAGT AGCAACCAGC AGGACCCAGG CGAGCCTGGG 
GAAGCAGAAG TGCAGGAGGA GGATCATGAT GTCACTCAGA CAGAGGTGGC AGATGGGGAT 
ATCATGGATG GGGAGGCTGA AACCGACTCA GTGGTGATTG CTGGGCAGCC TGAGGTGCTC 
AGTTCACAAG AGATGCAGGT TGAGAATGAG CTGGAGGACC TGATAGATGA GTTGCTTGAG 
AGGGATGGCG GATCTGGGAA CAGTACAATT ATAGTGAGCA GAAGTGGAGA GGATGAATCA 
CAAGAGGACG TGCTGATGGA TGAAGCTCCT TCCAACCTCA GCCAAGCTTC CACCTTGCAG 
GCCAACCGAG AAGATTCCAT GAATATCCTG GACCCTGAGG ATGAGGAGGA GCACACTCAG 
GAAGAGGACA GCAGTGGCAG TAACGAGGAT GAGGATGATA GTCAGGATGA AGAGGAGGAG 
GAGGAGGAAG ATGAGGAAGA TGATCAGGAG GATGATGAAG GTGAAGAGGG AGATGAAGAC 
GATGACGACG ATGGCTCTGA GATGGAATTG GATGAGGATT ATCCTGATAT GAACGCTTCT 
CCCTTGGTCC GATTTGAGCG CTTTGACCGG GAGGATGATC TCATCATTGA GTTTGACAAC 
ATGTTCTCCA GTGCTACAGA CATCCCCCCA TCCCCAGGAA ATATCCCTAC CACCCATCCA 
CTGATGGTGC GCCATGCAGA CCACAGTTCT CTGACACTGG GCAGTGGCTC TTCAACAACT 
CGTCTCACCC AGGGCATCGG GCGCAGTCAG AGGACCCTAA GGCAGCTGAC GGCCAATACT 
GGCCACACCA TTCATGTTCA CTACCCTGGG AATCGCCAGC CCAACCCTCC TCTTATACTG 
CAGAGGTTGC TTGGTCCCTC AGCTGCTGCT GACATCCTTC AGCTGAGCAG CAGCCTTCCC 
CTACAAAGCC GGGGTCGGGC CCGCCTCCTG GTAGGCAACG ATGACGTCCA CATCATCGCC 
CGTTCTGATG ATGAGCTGCT GGATGACTTT TTCCATGATC AGAGCACAGC TACCAGCCAA 
GCAGGAACCC TGTCCAGCAT CCCCACAGCC CTGACCCGCT GGACAGAAGA ATGCAAAGTT 
CTCGATGCTG AGAGCATGCA TGACTGTGTT TCAGTGGTTA AAGTGTCCAT TGTCAATCAC 
CTGGAATTCC TGAGGGATGA GGAGCTGGAA GAAAGGCGAG AGAAGCGCAG GAAACAACTG 
GCTGAGGAAG AAACAAAGAT AACTGATAAA GGCAAAGAAG ATAAGGAGAA CAGGGATCAG 
AGTGCACAGT GTACTGCATC TAAGTCAAAT GACTCCACTG AACAGAATCT CTCAGATGGG 
ACGCCTATGC CTGACAGCTA CCCAACAACC CCATCTTCAA CTGATGCAGC TACATCTGAG 
TCCAAGGAGA CCCTTGGCAC TCTGCAATCC TCACAACAGC AACCAACACT CCCAACCCCA 
CCAGCTTTGG GAGAGGTTCC TCAGGAGCTG CAGTCTCCAG CTGGAGAAGG GGGCAGCTCT 
ACACAGCTAT TGATGCCTGT AGAGCCAGAG GAATTGGGTC CCACAAGGCC AAGTGGGGAA 
GCAGAAACAA CTCAGATGGA GTTATCCCCA GCTCCCACTA TAACCTCACT TTCCCCAGAG 
AGAGCTGAGG ATTCTGATGC ACTGACGGCT GTCAGCAGTC AGCTAGAAGG CTCTCCTATG 
GATACAAGCA GCCTGGCTTC CTGTACCTTA GAGGAGGCTG TGGGTGACAC TTCAGCAGCT 
GGCAGTTCTG AGCAGCCCAG AGCAGGCAGC TCCACTCCTG GGGATGCCCC ACCAGCTGTG 
GCGGAAGTGC AAGGCAGGAG TGATGGGTCA GGGGAATCTG CCCAGCCACC TGAGGACAGC 
TCCCCACCTG CATCCTCTGA GAGCTCTTCC ACCAGAGATT CTGCCGTGGC CATTTCTGGA 
GCAGATTCCC GAGGAATCCT AGAAGAGCCG TTGCCTTCAA CAAGCAGTGA AGAAGAAGAT 
CCCCTTGCGG GTATCAGTCT CCCTGAAGGT GTGGACCCCT CTTTTCTGGC TGCCCTGCCT 
GATGACATCC GTCGGGAAGT TCTACAGAAC CAGCTAGGCA TTCGTCCACC AACCCGGACT 
GCCCCCTCCA CAAATAGCTC AGCGCCTGCA GTGGTGGGGA ATCCTGGTGT GACTGAAGTG 
AGCCCTGAGT TTCTGGCTGC CCTGCCTCCA GCCATTCAGG AGGAAGTACT GGCACAGCAG 
AGAGCTGAGC AGCAGCGACG AGAACTAGCA CAGAATGCCA GCTCAGACAC CCCTATGGAC 
CCTGTGACCT TCATCCAGAC TCTGCCCTCA GACCTGCGCC GTAGTGTCCT AGAGGATATG 
GAGGACAGTG TGTTAGCTGT GATGCCACCT GACATTGCAG CTGAGGCTCA AGCCCTGAGA 
CGAGAGCAAG AAGCCCGGCA GCGACAGCTC ATGCATGAGC GTCTGTTTGG GCACAGTAGC 
ACCTCCGCAC TCTCTGCTAT TCTCCGAAGC CCGGCTTTCA CCAGTCGCTT AAGTGGCAAC 
CGTGGGGTCC AGTATACTCG CCTTGCTGTG CAGAGAGGTG GCACCTTCCA GATGGGGGGT 
AGCAGCAGCC ATAACAGGCC TTCTGGCAGT AATGTAGATA CTCTCCTCCG CCTCCGAGGA 
CGGCTCCTTC TGGACCACGA AGCCCTTTCT TGTCTCTTGG TCCTACTTTT TGTGGATGAG 
CCAAAGCTCA ATACTAGCCG TCTACACCGA GTACTGAGAA ATCTCTGCTA CCATGCCCAG 
ACCCGCCACT GGGTCATCCG CAGTCTGCTC TCCATCTTGC AGCGCAGCAG TGAGAGTGAG 
CTATGCATTG AAACACCCAA ACTCACTACA AGTGAGGAAA AGGGCAAAAA GTCGAGCAAG 
AGCTGTGGGT CAAGTAGCCA TGAGAACCGT CCCCTGGACC TGCTACACAA GATGGAGTCA 
AAGAGCTCCA ACCAGCTTTC CTGGCTCTCA GTATCCATGG ATGCAGCCCT AGGCTGCAGG 
ACTAATATAT TTCAGATCCA GCGTTCAGGG GGGCGTAAAC ATACCGAGAA GCATGCAAGC 
GGTGGCTCCA CCGTCCACAT CCATCCCCAA GCTGCTCCTG TTGTCTGCAG ACACGTTTTG 
GATACACTCA TTCAATTGGC CAAGGTATTT CCCAGCCACT TCACACAGCA GCGGACCAAA 
GAAACAAACT GTGAGAGTGA TCGGGAAAGG GGCAATAAGG CCTGTAGCCC ATGCTCCTCA 
CAGTCCTCCA GCAGTGGCAT TTGCACAGAC TTCTGGGACT TATTGGTAAA ACTGGACAAC 
ATGAATGTCA GCCGGAAAGG CAAGAACTCC GTGAAGTCAG TGCCAGTGAG CGCTGGCGGT 
GAGGGGGAAA CCTCTCCATA CAGCCTCGAG GCCTCTCCAC TGGGGCAGCT CATGAACATG 
TTGTCACACC CAGTCATCCG CCGGAGCTCT CTCTTAACTG AGAAACTCCT CAGACTCCTT 
TCTCTCATCT CAATTGCTCT CCCAGAAAAC AAGGTGTCAG AAGCACAGGC TAATTCTGGC 
AGCGGTGCTT CCTCCACCAC CACTGCCACC TCAACCACAT CTACCACCAC CACCACTGCC 
GCCTCCACCA CGCCCACACC CCCTACTGCA CCCACCCCTG TCACTTCTGC TCCAGCCCTG 
GTTGCTGCCA CGGCTATTTC CACCATTGTC GTAGCTGCTT CGACCACAGT GACTACCCCC 
ACGACTGCTA CCACTACTGT TTCAATTTCT CCCACTACTA AGGGCAGCAA ATCTCCAGCG 
AAGGTGAGTG ATGGGGGCAG CAGCAGTACA GACTTTAAGA TGGTGTCCTC TGGCCTCACT 
GAAAACCAGC TACAGCTCTC TGTAGAGGTG TTGACATCCC ACTCTTGTTC TGAGGAAGGC 
TTAGAGGATG CAGCCAACGT ACTACTGCAG CTCTCCCGGG GGGACTCTGG GACCCGGGAC 
ACTGTTCTCA AGCTGCTACT GAATGGAGCC CGCCATCTGG GTTATACCCT TTGTAAACAA 
ATAGGTACCC TGCTGGCCGA GCTGCGGGAA TACAACCTCG AGCAGCAGCG GCGAGCCCAA 
TGTGAAACCC TCTCTCCTGA TGGCCTGCCT GAGGAGCAGC CACAGACCAC CAAGCTGAAG 
GGCAAAATGC AGAGCAGGTT TGACATGGCT GAGAATGTGG TAATTGTGGC ATCTCAGAAG 
CGACCTTTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA 
TCTACCCAGA AGTTCTTCTT GAGGGTACTA CAGGTCATCA TCCAGCTCCG GGACGACACG 
CGCCGGGCTA ACAAGAAAGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA 
GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA 
ATGGTACGTG AGGGTCAAAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCTAGC 
CAGTCAGAGG CGTCTGTCCG GAGGGAGGAA TCACCCATGG ATGTGGACCA GCCATCTCCC 
AGTGCTCAAG ATACTCAATC CATTGCCTCC GATGGAACCC CACAGGGGGA GAAGGAAAAG 
GAAGAAAGAC CACCTGAGTT ACCCCTGCTC AGCGAGCAGC TGAGTTTGGA CGAGCTGTGG 
GACATGCTTG GGGAGTGTCT AAAGGAACTA GAGGAATCCC ATGACCAGCA TGCGGTGCTA 
GTGCTACAGC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CCACAGAGCG GGAGAGCAAG 
CCTCCTGTCC GAGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCTCCTCCA 
CTCTCCCCTG CCCCCTTAAC CCCAGCCACG CCTTCCTCCC TTGACCCATT CTTCTCCCGG 
GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTCCTTCGC 
TTTGCAGAGA CTCACCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC GACCCACCTT 
GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT TGATGTCAAG 
CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTAGATGAGG GGCTCCGGAA AGAAGACATG 
GCTGTGCATG TCCGTCGTGA CCATGTGTTT GAAGACTCCT ATCGTGAGCT GCATCGCAAA 
TCCCCCGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT 
GCTGGTGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG 
TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGAGTCACCT ACACCATCAA TCCATCTTCC 
CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTCG GACGCATTGT GGCCAAAGCT 
GTATATGACA ACCGTCTTCT GGAGTGCTAC TTTACTCGAT CCTTTTACAA ACACATCTTG 
GGCAAGTCAG TCAGATATAC AGATATGGAG AGTGAAGATT ACCACTTCTA CCAAGGTCTG 
GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ACCTCACCTT CAGCACTGAG 
GTCCAAGAGT TTGGAGTTTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG 
GTAACAGAGG AGAATAAGAA GGAGTATGTA CACCTGGTAT GCCAGATGAG AATGACAGGA 
GCCATCCGCA AGCAGTTGGC GGCTTTCTTA GAAGGCTTCT ATGAGATCAT TCCAAAGCGC 
CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTTA TATCAGGACT GCCCACCATT 
GACATCGATG ATCTGAAATC CAACACTGAA TACCACAAGT ACCAGTCCAA CTCTATTCAG 
ATCCAGTGGT TCTGGAGAGC ATTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC 
CAGTTTGTCA CAGGTACTTC CAAGGTACCC CTGCAAGGCT TTGCTGCCCT CGAAGGCATG 
AATGGCATTC AGAAGTTTCA GATCCATCGA GATGACAGGT CCACAGATCG CCTGCCTTCA 
GCTCACACAT GTTTTAATCA GCTGGATCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC 
CACATGCTAC TGTTGGCTAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529049.1
CDS	398..13771
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_011530747.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530747.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAATCAGC AAACAGTATC 
TTGTATAATG GCTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACGGA TAAGCAGCTT 
ATGGAGATTA AAGCAGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT 
CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCA 
GTGCTTGTAA GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATACCCTCAC 
CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC 
GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGATA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT 
GAGCATGAAG TAGATTTGTG CCGAAAAGAA TGTCCGTTTG TGATCAAGCC AAAGATCCAG 
AGACCCAATA CTACACAAGA AGGAGAGGAA ATGGAAACTG ATATGGATGT TGCAGATGTG 
GCTATGGAAA GCAGTCCAGG CTCATCCATC TCTATGGAGC ACAGGCTGGA TGTTGAATTA 
AGGGCATCAG GTTCCAGCAG CAGCACTAAC ATCTCTTCTG GCCCCAGCCC TGGTCCCAGT 
CCCGGCCCCG GCACCGGCCC TGGCCCCGGC CCCGGCCCCG GCCCCGGCCC TGGCCCCGGC 
CCCGGCCCCG GTCCTGGTCC CGGCCCTGGC CCCGGCCCTG GCCCTGGCCC CCGTCCTGGA 
GTCCAGTGTA TTCCACAACG AGCAGCACTT CTGAAATCCA TGTTGAATTT CCTCAAGAAG 
GCCATCCAAG ACCCTGCTTT CTCAGATGGC ATACGACATG TGATGGATGG TTCTCTGCCT 
ACCTCCCTGA AACACATCAT CAGCAATGCA GAATACTATG GCCCATCACT CTTCCTCCTA 
GCTACTGAAG TGGTGACTGT GTTTGTATTT CAAGAACCAT CACTGCTCTC CTCACTCCAG 
GACAATGGAT TGACAGATGT CATGCTGCAT GCACTGCTTA TCAAAGATGT TCCTGCTACC 
CGTGAAGTCC TTGGCTCCCT CCCAAATGTA TTCAGTGCAC TCTGTTTGAA TGCCCGAGGT 
CTTCAGTCTT TTGTTCAGTG TCAGCCTTTT GAACGCCTCT TCAAAGTTCT TCTGTCTCCA 
GATTACCTCC CAGCCATGCG GAGGAGGAGA AGTTCTGATC CCCTTGGGGA TACTGCATCC 
AACCTGGGGA GTGCTGTCGA TGAGCTCATG AGACATCAGC CCACCCTTAA AACAGATGCA 
ACGACTGCCA TCATCAAGTT ACTTGAAGAA ATCTGTAATC TTGGAAGGGA CCCCAAATAC 
ATCTGTCAGA AGCCATCAAT CCAGAAGGCA GATGGCACTG CCACTGCTCC TCCCCCAAGG 
TCTAATCATG CCGCAGAAGA AGCCTCTAGT GAGGATGAGG AGGAAGAGGA AGTACAGGCC 
ATGCAGAGCT TTAATTCTAC CCAGCAAAAT GAAACTGAGC CTAATCAGCA GGTTGTTGGT 
ACAGAGGAAC GTATTCCTAT TCCCCTCATG GATTACATCC TTAATGTGAT GAAATTTGTG 
GAATCTATTC TGAGCAACAA TACAACAGAT GACCACTGCC AGGAATTTGT GAATCAGAAA 
GGACTGTTGC CTTTGGTTAC CATTTTGGGT CTTCCCAATC TGCCCATTGA CTTTCCCACA 
TCTGCTGCCT GTCAGGCTGT TGCAGGTGTC TGCAAATCCA TATTGACACT GTCACATGAA 
CCCAAAGTCC TTCAAGAGGG TCTCCTTCAG TTGGACTCCA TCCTCTCCTC CCTGGAGCCC 
TTACACCGCC CCATTGAATC CCCTGGGGGC TCAGTGTTGT TGCGAGAACT GGCTTGCGCA 
GGCAATGTTG CTGATGCTAC CCTCTCAGCC CAGGCCACAC CTCTGCTGCA TGCACTCACT 
GCTGCCCATG CCTACATCAT GATGTTTGTT CATACTTGCA GAGTTGGACA GAGTGAAATT 
CGTTCCATCT CCGTAAACCA GTGGGGCTCT CAATTGGGTC TGAGTGTTTT GAGCAAGCTG 
AGCCAGTTAT ACTGTTCCCT GGTGTGGGAA AGCACTGTCC TCCTCTCTCT GTGTACCCCA 
AACAGCCTAC CATCTGGGTG TGAATTTGGC CAGGCAGATA TGCAGAAACT GGTTCCAAAG 
GATGAGAAGG CAGGTACGAC CCAGGGCGGA AAAAGATCAG ATGGGGAACA GGATGGAGCA 
GCTGGAAGTA TGGATGCTTC TACCCAGGGC TTATTAGAAG GCATTGGGCT AGATGGTGAC 
ACATTGGCTC CCATGGAGAC AGATGAACCT ACTGCTTCAG ACTCTAAGGG CAAATCTAAA 
ATCACACCAG CAATGGCTGC CAGAATTAAG CAAATCAAGC CTTTGTTATC AGCTTCCTCC 
AGATTAGGCC GAGCACTTGC TGAGCTATTT GGACTTCTTG TTAAACTTTG TGTGGGATCT 
CCTGTCCGCC AGAGAAGGAG CCATCATGCT GCCAGCACCA CTACAGCACC GACACCTGCC 
GCGCGATCAA CAGCCTCAGC TCTCACTAAG CTCTTGACTA AGGGGTTATC TTGGCAGCCC 
CCACCATATA CACCTACTCC CCGATTCAGG CTGACATTCT TCATCTGTTC AGTTGGTTTC 
ACATCCCCAA TGCTGTTTGA TGAGAGGAAG TATCCCTACC ACCTCATGCT GCAAAAATTT 
CTCTGCTCCG GAGGCCACAA TGCTCTTTTT GAAACTTTCA ACTGGGCTCT GTCCATGGGA 
GGTAAAGTTC CTGTTTCTGA GGGATTGGAA CACTCAGACT TGCCTGATGG CACAGGAGAA 
TTCCTAGATG CCTGGCTTAT GCTGGTGGAG AAGATGGTGA ATCCCACCAC GGTGCTTGAA 
TCTCCACATT CGCTGCCTGC CAAATTGCCT GGAGGTGTCC AGAACTTTCC CCAGTTCAGT 
GCACTGCGCT TCCTTGTGGT AACTCAGAAA GCAGCCTTTA CTTGCATCAA AAACTTATGG 
AACCGGAAAC CCCTGAAGGT ATATGGTGGA CGAATGGCTG AATCGATGCT GGCCATTCTA 
TGCCACATCC TCCGAGGAGA ACCTGTGATT CGAGAGAGAC TAAGCAAGGA GAAGGAGGGG 
TCTCGAGGAG AAGAGGATAC AGGGCAAGAG GAAGGTGGCT CCCGCCGGGA ACCTCAAGTC 
AACCAGCAAC AACTGCAACA GCTCATGGAC ATGGGCTTCA CAAGGGAACA TGCAATGGAG 
GCACTGTTGA ACACCAGCAC CATGGAGCAG GCCACAGAGT ACCTTTTAAC CCACCCTCCT 
CCAATCATGG GAGGAGTTGT TCGGGATCTC AGCATGTCTG AAGAGGACCA GATGATGAGA 
GCAATTGCTA TGTCTCTGGG ACAGGATATT CCAATGGATC AAAGGGCAGA GTCACCTGAG 
GAAGTTGCTT GCCGGAAGGA GGAAGAGGAA CGGAAAGCTC GGGAAAAGCA GGAGGAGGAA 
GAGGCTAAAT GTCTAGAGAA GTTCCAGGAT GCTGACCCGT TGGAACAAGA TGAGCTCCAC 
ACTTTCACAG ATACTATGTT GCCAGGCTGC TTCCACCTTC TTGATGAGCT GCCAGACACA 
GTATACCGTG TGTGTGACCT GATCATGACA GCAATCAAAC GTAATGGAGC AGATTATCGT 
GACATGATTC TGAAGCAAGT AGTCAATCAG GTGTGGGAAG CTGCTGATGT ATTGATCAAA 
GCTGCTCTTC CCCTGACAAC AAGTGACACA AAAACCGTGT CAGAGTGGAT AAGTCAGATG 
GCCACACTGC CCCAGGCCTC CAATTTGGCT ACTAGAATCT TGCTTTTAAC GCTACTTTTT 
GAGGAGTTGA AGCTACCTTG TGCTTGGGTG GTTGAATCAA GTGGCATCCT TAATGTCCTA 
ATCAAACTCT TGGAAGTGGT TCAGCCCTGC CTCCAGGCAG CCAAGGAGCA GAAGGAAGTC 
CAGACCCCAA AGTGGATCAC ACCAGTGTTG CTCCTGATTG ATTTCTATGA AAAGACAGCC 
ATCTCCTCAA AAAGGAGAGC CCAGATGACT AAGTACCTGC AATCCAACAG CAACAACTGG 
CGCTGGTTTG ATGATCGCTC TGGGCGTTGG TGTAGTTACA GTGCAAGCAA CAATAGCACT 
ATTGATTCTG CCTGGAAATC TGGAGAGACA AGCGTGCGAT TCACTGCAGG CCGAAGAAGA 
TACACGGTCC AATTCACTAC AATGGTGCAG GTTAATGAGG AAACAGGGAA CCGACGCCCT 
GTGATGCTGA CTCTCCTCAG GGTACCTCGG CTGAATAAAA ATTCAAAAAA CAGCAATGGA 
CAGGAACTAG AGAAGACGCT GGAAGAAAGC AAAGAAATGG ATATCAAACG TAAAGAAAAT 
AAAGGCAATG ATACCCCTTT GGCCCTAGAG AGTACAAACA CTGAAAAGGA GACAAGCCTG 
GAGGAAACAA AAATCGGGGA GATCCTGATC CAGGGCTTGA CAGAAGATAT GGTGACTGTT 
TTAATCCGGG CCTGCGTGAG CATGCTGGGA GTCCCTGTGG ACCCAGATAC TTTGCATGCC 
ACCCTTCGTC TCTGTCTGAG GCTCACCCGG GACCACAAAT ATGCCATGAT GTTTGCAGAA 
CTGAAGAGTA CCCGCATGAT CTTGAATTTG ACCCAGAGCT CAGGCTTCAA TGGGTTTACT 
CCCCTGGTCA CCCTTCTCTT AAGACACATC ATTGAGGACC CCTGTACCCT TCGTCATACC 
ATGGAAAAGG TTGTTCGCTC AGCAGCTACA AGTGGAGCTG GTAGCACTAC CTCTGGTGTT 
GTGTCTGGCA GCCTCGGCTC TCGGGAGATC AACTACATCC TTCGTGTCCT TGGGCCAGCC 
GCATGCCGCA ATCCAGACAT ATTCACAGAA GTGGCCAACT GCTGTATCCG CATCGCCCTT 
CCTGCCCCTC GAGGCTCAGG AACTGCTTCA GATGATGAAT TTGAGAATCT TAGAATTAAA 
GGCCCTAATG CTGTACAGCT GGTGAAGACC ACCCCTTTGA AGCCCTCACC TCTGCCTGTC 
ATCCCTGATA CTATCAAGGA AGTGATCTAT GATATGCTGA ATGCTCTGGC TGCATACCAT 
GCTCCAGAGG AAGCAGATAA ATCTGATCCT AAACCTGGGG TTATGACCCA AGAGGTTGGC 
CAGCTCCTGC AAGACATGGG TGATGATGTA TACCAGCAGT ACCGGTCACT TACGCGTCAG 
AGCAGTGACT TTGATACGCA GTCAGGTTTT TCCATTAATA GTCAGGTCTT TGCTGCAGAT 
GGTGCCTCCA CTGAGACTTC CGCATCTGGG ACCTCCCAAG GAGAGGCTTC AACTCCAGAG 
GAGTCTCGAG ATGGGAAGAA AGATAAAGAA GGGGACCGGG CCTCTGAGGA AGGCAAACAG 
AAAGGCAAGG GCAGCAAACC TTTAATGCCT ACCTCCACTA TCCTTCGTCT TCTGGCAGAG 
TTGGTGAGGT CCTATGTTGG TATTGCTACC CTGATTGCCA ACTACAGCTA CACTGTGGGC 
CAGTCTGAAC TGATCAAAGA GGACTGCAGT GTGCTAGCTT TTGTTCTGGA CCACCTGCTC 
CCACATACCC AGAATGCAGA AGACAAGGAC ACCCCTGCCT TGGCCCGCCT GTTCCTCGCA 
AGCCTGGCTG CTGCAGGGAG TGGCACAGAT GCCCAGGTGG CCCTAGTGAA TGAAGTAAAA 
GCAGCCCTTG GACGGGCACT GGCTATGGCT GAGAGTACAG AGAAACATGC CAGGCTTCAG 
GCAGTGATGT GTATCATCAG TACTATCATG GAGTCCTGCC CCTCCACCTC CAGCTTCTAC 
AGCAGTGCCA CAGCGAAGAC CCAGCACAAT GGCATGAACA ACATCATTCG GCTTTTCCTG 
AAGAAGGGAC TGGTTAATGA CCTGGCCAGA GTACCTCACA GCTTAGACCT GTCCAGTCCC 
AACATGGCCA ACACAGTCAA TGCTGCTCTG AAGCCTTTGG AAACACTTTC CCGGATTGTG 
AACCAGCCCA GTAGCCTTTT TGGCAGCAAG AGTGCTTCTA GCAAGAACAA GTCTGAGCAG 
GATGCCCAAG GAGCCTCTCA AGATTCCAGT AGCAACCAGC AGGACCCAGG CGAGCCTGGG 
GAAGCAGAAG TGCAGGAGGA GGATCATGAT GTCACTCAGA CAGAGGTGGC AGATGGGGAT 
ATCATGGATG GGGAGGCTGA AACCGACTCA GTGGTGATTG CTGGGCAGCC TGAGGTGCTC 
AGTTCACAAG AGATGCAGGT TGAGAATGAG CTGGAGGACC TGATAGATGA GTTGCTTGAG 
AGGGATGGCG GATCTGGGAA CAGTACAATT ATAGTGAGCA GAAGTGGAGA GGATGAATCA 
CAAGAGGACG TGCTGATGGA TGAAGCTCCT TCCAACCTCA GCCAAGCTTC CACCTTGCAG 
GCCAACCGAG AAGATTCCAT GAATATCCTG GACCCTGAGG ATGAGGAGGA GCACACTCAG 
GAAGAGGACA GCAGTGGCAG TAACGAGGAT GAGGATGATA GTCAGGATGA AGAGGAGGAG 
GAGGAGGAAG ATGAGGAAGA TGATCAGGAG GATGATGAAG GTGAAGAGGG AGATGAAGAC 
GATGACGACG ATGGCTCTGA GATGGAATTG GATGAGGATT ATCCTGATAT GAACGCTTCT 
CCCTTGGTCC GATTTGAGCG CTTTGACCGG GAGGATGATC TCATCATTGA GTTTGACAAC 
ATGTTCTCCA GTGCTACAGA CATCCCCCCA TCCCCAGGAA ATATCCCTAC CACCCATCCA 
CTGATGGTGC GCCATGCAGA CCACAGTTCT CTGACACTGG GCAGTGGCTC TTCAACAACT 
CGTCTCACCC AGGGCATCGG GCGCAGTCAG AGGACCCTAA GGCAGCTGAC GGCCAATACT 
GGCCACACCA TTCATGTTCA CTACCCTGGG AATCGCCAGC CCAACCCTCC TCTTATACTG 
CAGAGGTTGC TTGGTCCCTC AGCTGCTGCT GACATCCTTC AGCTGAGCAG CAGCCTTCCC 
CTACAAAGCC GGGGTCGGGC CCGCCTCCTG GTAGGCAACG ATGACGTCCA CATCATCGCC 
CGTTCTGATG ATGAGCTGCT GGATGACTTT TTCCATGATC AGAGCACAGC TACCAGCCAA 
GCAGGAACCC TGTCCAGCAT CCCCACAGCC CTGACCCGCT GGACAGAAGA ATGCAAAGTT 
CTCGATGCTG AGAGCATGCA TGACTGTGTT TCAGTGGTTA AAGTGTCCAT TGTCAATCAC 
CTGGAATTCC TGAGGGATGA GGAGCTGGAA GAAAGGCGAG AGAAGCGCAG GAAACAACTG 
GCTGAGGAAG AAACAAAGAT AACTGATAAA GGCAAAGAAG ATAAGGAGAA CAGGGATCAG 
AGTGCACAGT GTACTGCATC TAAGTCAAAT GACTCCACTG AACAGAATCT CTCAGATGGG 
ACGCCTATGC CTGACAGCTA CCCAACAACC CCATCTTCAA CTGATGCAGC TACATCTGAG 
TCCAAGGAGA CCCTTGGCAC TCTGCAATCC TCACAACAGC AACCAACACT CCCAACCCCA 
CCAGCTTTGG GAGAGGTTCC TCAGGAGCTG CAGTCTCCAG CTGGAGAAGG GGGCAGCTCT 
ACACAGCTAT TGATGCCTGT AGAGCCAGAG GAATTGGGTC CCACAAGGCC AAGTGGGGAA 
GCAGAAACAA CTCAGATGGA GTTATCCCCA GCTCCCACTA TAACCTCACT TTCCCCAGAG 
AGAGCTGAGG ATTCTGATGC ACTGACGGCT GTCAGCAGTC AGCTAGAAGG CTCTCCTATG 
GATACAAGCA GCCTGGCTTC CTGTACCTTA GAGGAGGCTG TGGGTGACAC TTCAGCAGCT 
GGCAGTTCTG AGCAGCCCAG AGCAGGCAGC TCCACTCCTG GGGATGCCCC ACCAGCTGTG 
GCGGAAGTGC AAGGCAGGAG TGATGGGTCA GGGGAATCTG CCCAGCCACC TGAGGACAGC 
TCCCCACCTG CATCCTCTGA GAGCTCTTCC ACCAGAGATT CTGCCGTGGC CATTTCTGGA 
GCAGATTCCC GAGGAATCCT AGAAGAGCCG TTGCCTTCAA CAAGCAGTGA AGAAGAAGAT 
CCCCTTGCGG GTATCAGTCT CCCTGAAGGT GTGGACCCCT CTTTTCTGGC TGCCCTGCCT 
GATGACATCC GTCGGGAAGT TCTACAGAAC CAGCTAGGCA TTCGTCCACC AACCCGGACT 
GCCCCCTCCA CAAATAGCTC AGCGCCTGCA GTGGTGGGGA ATCCTGGTGT GACTGAAGTG 
AGCCCTGAGT TTCTGGCTGC CCTGCCTCCA GCCATTCAGG AGGAAGTACT GGCACAGCAG 
AGAGCTGAGC AGCAGCGACG AGAACTAGCA CAGAATGCCA GCTCAGACAC CCCTATGGAC 
CCTGTGACCT TCATCCAGAC TCTGCCCTCA GACCTGCGCC GTAGTGTCCT AGAGGATATG 
GAGGACAGTG TGTTAGCTGT GATGCCACCT GACATTGCAG CTGAGGCTCA AGCCCTGAGA 
CGAGAGCAAG AAGCCCGGCA GCGACAGCTC ATGCATGAGC GTCTGTTTGG GCACAGTAGC 
ACCTCCGCAC TCTCTGCTAT TCTCCGAAGC CCGGCTTTCA CCAGTCGCTT AAGTGGCAAC 
CGTGGGGTCC AGTATACTCG CCTTGCTGTG CAGAGAGGTG GCACCTTCCA GATGGGGGGT 
AGCAGCAGCC ATAACAGGCC TTCTGGCAGT AATGTAGATA CTCTCCTCCG CCTCCGAGGA 
CGGCTCCTTC TGGACCACGA AGCCCTTTCT TGTCTCTTGG TCCTACTTTT TGTGGATGAG 
CCAAAGCTCA ATACTAGCCG TCTACACCGA GTACTGAGAA ATCTCTGCTA CCATGCCCAG 
ACCCGCCACT GGGTCATCCG CAGTCTGCTC TCCATCTTGC AGCGCAGCAG TGAGAGTGAG 
CTATGCATTG AAACACCCAA ACTCACTACA AGTGAGGAAA AGGGCAAAAA GTCGAGCAAG 
AGCTGTGGGT CAAGTAGCCA TGAGAACCGT CCCCTGGACC TGCTACACAA GATGGAGTCA 
AAGAGCTCCA ACCAGCTTTC CTGGCTCTCA GTATCCATGG ATGCAGCCCT AGGCTGCAGG 
ACTAATATAT TTCAGATCCA GCGTTCAGGG GGGCGTAAAC ATACCGAGAA GCATGCAAGC 
GGTGGCTCCA CCGTCCACAT CCATCCCCAA GCTGCTCCTG TTGTCTGCAG ACACGTTTTG 
GATACACTCA TTCAATTGGC CAAGGTATTT CCCAGCCACT TCACACAGCA GCGGACCAAA 
GAAACAAACT GTGAGAGTGA TCGGGAAAGG GGCAATAAGG CCTGTAGCCC ATGCTCCTCA 
CAGTCCTCCA GCAGTGGCAT TTGCACAGAC TTCTGGGACT TATTGGTAAA ACTGGACAAC 
ATGAATGTCA GCCGGAAAGG CAAGAACTCC GTGAAGTCAG TGCCAGTGAG CGCTGGCGGT 
GAGGGGGAAA CCTCTCCATA CAGCCTCGAG GCCTCTCCAC TGGGGCAGCT CATGAACATG 
TTGTCACACC CAGTCATCCG CCGGAGCTCT CTCTTAACTG AGAAACTCCT CAGACTCCTT 
TCTCTCATCT CAATTGCTCT CCCAGAAAAC AAGGTGTCAG AAGCACAGGC TAATTCTGGC 
AGCGGTGCTT CCTCCACCAC CACTGCCACC TCAACCACAT CTACCACCAC CACCACTGCC 
GCCTCCACCA CGCCCACACC CCCTACTGCA CCCACCCCTG TCACTTCTGC TCCAGCCCTG 
GTTGCTGCCA CGGCTATTTC CACCATTGTC GTAGCTGCTT CGACCACAGT GACTACCCCC 
ACGACTGCTA CCACTACTGT TTCAATTTCT CCCACTACTA AGGGCAGCAA ATCTCCAGCG 
AAGGTGAGTG ATGGGGGCAG CAGCAGTACA GACTTTAAGA TGGTGTCCTC TGGCCTCACT 
GAAAACCAGC TACAGCTCTC TGTAGAGGTG TTGACATCCC ACTCTTGTTC TGAGGAAGGC 
TTAGAGGATG CAGCCAACGT ACTACTGCAG CTCTCCCGGG GGGACTCTGG GACCCGGGAC 
ACTGTTCTCA AGCTGCTACT GAATGGAGCC CGCCATCTGG GTTATACCCT TTGTAAACAA 
ATAGGTACCC TGCTGGCCGA GCTGCGGGAA TACAACCTCG AGCAGCAGCG GCGAGCCCAA 
TGTGAAACCC TCTCTCCTGA TGGCCTGCCT GAGGAGCAGC CACAGACCAC CAAGCTGAAG 
GGCAAAATGC AGAGCAGGTT TGACATGGCT GAGAATGTGG TAATTGTGGC ATCTCAGAAG 
CGACCTTTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA 
TCTACCCAGA AGTTCTTCTT GAGGGTACTA CAGGTCATCA TCCAGCTCCG GGACGACACG 
CGCCGGGCTA ACAAGAAAGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA 
GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA 
ATGGTACGTG AGGGTCAAAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCTAGC 
CAGTCAGAGG CGTCTGTCCG GAGGGAGGAA TCACCCATGG ATGTGGACCA GCCATCTCCC 
AGTGCTCAAG ATACTCAATC CATTGCCTCC GATGGAACCC CACAGGGGGA GAAGGAAAAG 
GAAGAAAGAC CACCTGAGTT ACCCCTGCTC AGCGAGCAGC TGAGTTTGGA CGAGCTGTGG 
GACATGCTTG GGGAGTGTCT AAAGGAACTA GAGGAATCCC ATGACCAGCA TGCGGTGCTA 
GTGCTACAGC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CCACAGAGCG GGAGAGCAAG 
CCTCCTGTCC GAGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCTCCTCCA 
CTCTCCCCTG CCCCCTTAAC CCCAGCCACG CCTTCCTCCC TTGACCCATT CTTCTCCCGG 
GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTCCTTCGC 
TTTGCAGAGA CTCACCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC GACCCACCTT 
GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT TGATGTCAAG 
CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTAGATGAGG GGCTCCGGAA AGAAGACATG 
GCTGTGCATG TCCGTCGTGA CCATGTGTTT GAAGACTCCT ATCGTGAGCT GCATCGCAAA 
TCCCCCGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT 
GCTGGTGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG 
TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGAGTCACCT ACACCATCAA TCCATCTTCC 
CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTCG GACGCATTGT GGCCAAAGCT 
GTATATGACA ACCGTCTTCT GGAGTGCTAC TTTACTCGAT CCTTTTACAA ACACATCTTG 
GGCAAGTCAG TCAGATATAC AGATATGGAG AGTGAAGATT ACCACTTCTA CCAAGGTCTG 
GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ACCTCACCTT CAGCACTGAG 
GTCCAAGAGT TTGGAGTTTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG 
GTAACAGAGG AGAATAAGAA GGAGTATGTA CACCTGGTAT GCCAGATGAG AATGACAGGA 
GCCATCCGCA AGCAGTTGGC GGCTTTCTTA GAAGGCTTCT ATGAGATCAT TCCAAAGCGC 
CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTTA TATCAGGACT GCCCACCATT 
GACATCGATG ATCTGAAATC CAACACTGAA TACCACAAGT ACCAGTCCAA CTCTATTCAG 
ATCCAGTGGT TCTGGAGAGC ATTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC 
CAGTTTGTCA CAGGTACTTC CAAGGTACCC CTGCAAGGCT TTGCTGCCCT CGAAGGCATG 
AATGGCATTC AGAAGTTTCA GATCCATCGA GATGACAGGT CCACAGATCG CCTGCCTTCA 
GCTCACACAT GTTTTAATCA GCTGGATCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC 
CACATGCTAC TGTTGGCTAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_024452322.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308090.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X15, mRNA.

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CloneID	OHu63653
Clone ID Related Accession (Same CDS sequence)	XM_011530747.1 , XM_024452322.1 , XM_011530751.2
Accession Version	XM_024452322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAATCAGC AAACAGTATC 
TTGTATAATG GCTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACGGA TAAGCAGCTT 
ATGGAGATTA AAGCAGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT 
CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCA 
GTGCTTGTAA GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATACCCTCAC 
CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC 
GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGATA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT 
GAGCATGAAG TAGATTTGTG CCGAAAAGAA TGTCCGTTTG TGATCAAGCC AAAGATCCAG 
AGACCCAATA CTACACAAGA AGGAGAGGAA ATGGAAACTG ATATGGATGT TGCAGATGTG 
GCTATGGAAA GCAGTCCAGG CTCATCCATC TCTATGGAGC ACAGGCTGGA TGTTGAATTA 
AGGGCATCAG GTTCCAGCAG CAGCACTAAC ATCTCTTCTG GCCCCAGCCC TGGTCCCAGT 
CCCGGCCCCG GCACCGGCCC TGGCCCCGGC CCCGGCCCCG GCCCCGGCCC TGGCCCCGGC 
CCCGGCCCCG GTCCTGGTCC CGGCCCTGGC CCCGGCCCTG GCCCTGGCCC CCGTCCTGGA 
GTCCAGTGTA TTCCACAACG AGCAGCACTT CTGAAATCCA TGTTGAATTT CCTCAAGAAG 
GCCATCCAAG ACCCTGCTTT CTCAGATGGC ATACGACATG TGATGGATGG TTCTCTGCCT 
ACCTCCCTGA AACACATCAT CAGCAATGCA GAATACTATG GCCCATCACT CTTCCTCCTA 
GCTACTGAAG TGGTGACTGT GTTTGTATTT CAAGAACCAT CACTGCTCTC CTCACTCCAG 
GACAATGGAT TGACAGATGT CATGCTGCAT GCACTGCTTA TCAAAGATGT TCCTGCTACC 
CGTGAAGTCC TTGGCTCCCT CCCAAATGTA TTCAGTGCAC TCTGTTTGAA TGCCCGAGGT 
CTTCAGTCTT TTGTTCAGTG TCAGCCTTTT GAACGCCTCT TCAAAGTTCT TCTGTCTCCA 
GATTACCTCC CAGCCATGCG GAGGAGGAGA AGTTCTGATC CCCTTGGGGA TACTGCATCC 
AACCTGGGGA GTGCTGTCGA TGAGCTCATG AGACATCAGC CCACCCTTAA AACAGATGCA 
ACGACTGCCA TCATCAAGTT ACTTGAAGAA ATCTGTAATC TTGGAAGGGA CCCCAAATAC 
ATCTGTCAGA AGCCATCAAT CCAGAAGGCA GATGGCACTG CCACTGCTCC TCCCCCAAGG 
TCTAATCATG CCGCAGAAGA AGCCTCTAGT GAGGATGAGG AGGAAGAGGA AGTACAGGCC 
ATGCAGAGCT TTAATTCTAC CCAGCAAAAT GAAACTGAGC CTAATCAGCA GGTTGTTGGT 
ACAGAGGAAC GTATTCCTAT TCCCCTCATG GATTACATCC TTAATGTGAT GAAATTTGTG 
GAATCTATTC TGAGCAACAA TACAACAGAT GACCACTGCC AGGAATTTGT GAATCAGAAA 
GGACTGTTGC CTTTGGTTAC CATTTTGGGT CTTCCCAATC TGCCCATTGA CTTTCCCACA 
TCTGCTGCCT GTCAGGCTGT TGCAGGTGTC TGCAAATCCA TATTGACACT GTCACATGAA 
CCCAAAGTCC TTCAAGAGGG TCTCCTTCAG TTGGACTCCA TCCTCTCCTC CCTGGAGCCC 
TTACACCGCC CCATTGAATC CCCTGGGGGC TCAGTGTTGT TGCGAGAACT GGCTTGCGCA 
GGCAATGTTG CTGATGCTAC CCTCTCAGCC CAGGCCACAC CTCTGCTGCA TGCACTCACT 
GCTGCCCATG CCTACATCAT GATGTTTGTT CATACTTGCA GAGTTGGACA GAGTGAAATT 
CGTTCCATCT CCGTAAACCA GTGGGGCTCT CAATTGGGTC TGAGTGTTTT GAGCAAGCTG 
AGCCAGTTAT ACTGTTCCCT GGTGTGGGAA AGCACTGTCC TCCTCTCTCT GTGTACCCCA 
AACAGCCTAC CATCTGGGTG TGAATTTGGC CAGGCAGATA TGCAGAAACT GGTTCCAAAG 
GATGAGAAGG CAGGTACGAC CCAGGGCGGA AAAAGATCAG ATGGGGAACA GGATGGAGCA 
GCTGGAAGTA TGGATGCTTC TACCCAGGGC TTATTAGAAG GCATTGGGCT AGATGGTGAC 
ACATTGGCTC CCATGGAGAC AGATGAACCT ACTGCTTCAG ACTCTAAGGG CAAATCTAAA 
ATCACACCAG CAATGGCTGC CAGAATTAAG CAAATCAAGC CTTTGTTATC AGCTTCCTCC 
AGATTAGGCC GAGCACTTGC TGAGCTATTT GGACTTCTTG TTAAACTTTG TGTGGGATCT 
CCTGTCCGCC AGAGAAGGAG CCATCATGCT GCCAGCACCA CTACAGCACC GACACCTGCC 
GCGCGATCAA CAGCCTCAGC TCTCACTAAG CTCTTGACTA AGGGGTTATC TTGGCAGCCC 
CCACCATATA CACCTACTCC CCGATTCAGG CTGACATTCT TCATCTGTTC AGTTGGTTTC 
ACATCCCCAA TGCTGTTTGA TGAGAGGAAG TATCCCTACC ACCTCATGCT GCAAAAATTT 
CTCTGCTCCG GAGGCCACAA TGCTCTTTTT GAAACTTTCA ACTGGGCTCT GTCCATGGGA 
GGTAAAGTTC CTGTTTCTGA GGGATTGGAA CACTCAGACT TGCCTGATGG CACAGGAGAA 
TTCCTAGATG CCTGGCTTAT GCTGGTGGAG AAGATGGTGA ATCCCACCAC GGTGCTTGAA 
TCTCCACATT CGCTGCCTGC CAAATTGCCT GGAGGTGTCC AGAACTTTCC CCAGTTCAGT 
GCACTGCGCT TCCTTGTGGT AACTCAGAAA GCAGCCTTTA CTTGCATCAA AAACTTATGG 
AACCGGAAAC CCCTGAAGGT ATATGGTGGA CGAATGGCTG AATCGATGCT GGCCATTCTA 
TGCCACATCC TCCGAGGAGA ACCTGTGATT CGAGAGAGAC TAAGCAAGGA GAAGGAGGGG 
TCTCGAGGAG AAGAGGATAC AGGGCAAGAG GAAGGTGGCT CCCGCCGGGA ACCTCAAGTC 
AACCAGCAAC AACTGCAACA GCTCATGGAC ATGGGCTTCA CAAGGGAACA TGCAATGGAG 
GCACTGTTGA ACACCAGCAC CATGGAGCAG GCCACAGAGT ACCTTTTAAC CCACCCTCCT 
CCAATCATGG GAGGAGTTGT TCGGGATCTC AGCATGTCTG AAGAGGACCA GATGATGAGA 
GCAATTGCTA TGTCTCTGGG ACAGGATATT CCAATGGATC AAAGGGCAGA GTCACCTGAG 
GAAGTTGCTT GCCGGAAGGA GGAAGAGGAA CGGAAAGCTC GGGAAAAGCA GGAGGAGGAA 
GAGGCTAAAT GTCTAGAGAA GTTCCAGGAT GCTGACCCGT TGGAACAAGA TGAGCTCCAC 
ACTTTCACAG ATACTATGTT GCCAGGCTGC TTCCACCTTC TTGATGAGCT GCCAGACACA 
GTATACCGTG TGTGTGACCT GATCATGACA GCAATCAAAC GTAATGGAGC AGATTATCGT 
GACATGATTC TGAAGCAAGT AGTCAATCAG GTGTGGGAAG CTGCTGATGT ATTGATCAAA 
GCTGCTCTTC CCCTGACAAC AAGTGACACA AAAACCGTGT CAGAGTGGAT AAGTCAGATG 
GCCACACTGC CCCAGGCCTC CAATTTGGCT ACTAGAATCT TGCTTTTAAC GCTACTTTTT 
GAGGAGTTGA AGCTACCTTG TGCTTGGGTG GTTGAATCAA GTGGCATCCT TAATGTCCTA 
ATCAAACTCT TGGAAGTGGT TCAGCCCTGC CTCCAGGCAG CCAAGGAGCA GAAGGAAGTC 
CAGACCCCAA AGTGGATCAC ACCAGTGTTG CTCCTGATTG ATTTCTATGA AAAGACAGCC 
ATCTCCTCAA AAAGGAGAGC CCAGATGACT AAGTACCTGC AATCCAACAG CAACAACTGG 
CGCTGGTTTG ATGATCGCTC TGGGCGTTGG TGTAGTTACA GTGCAAGCAA CAATAGCACT 
ATTGATTCTG CCTGGAAATC TGGAGAGACA AGCGTGCGAT TCACTGCAGG CCGAAGAAGA 
TACACGGTCC AATTCACTAC AATGGTGCAG GTTAATGAGG AAACAGGGAA CCGACGCCCT 
GTGATGCTGA CTCTCCTCAG GGTACCTCGG CTGAATAAAA ATTCAAAAAA CAGCAATGGA 
CAGGAACTAG AGAAGACGCT GGAAGAAAGC AAAGAAATGG ATATCAAACG TAAAGAAAAT 
AAAGGCAATG ATACCCCTTT GGCCCTAGAG AGTACAAACA CTGAAAAGGA GACAAGCCTG 
GAGGAAACAA AAATCGGGGA GATCCTGATC CAGGGCTTGA CAGAAGATAT GGTGACTGTT 
TTAATCCGGG CCTGCGTGAG CATGCTGGGA GTCCCTGTGG ACCCAGATAC TTTGCATGCC 
ACCCTTCGTC TCTGTCTGAG GCTCACCCGG GACCACAAAT ATGCCATGAT GTTTGCAGAA 
CTGAAGAGTA CCCGCATGAT CTTGAATTTG ACCCAGAGCT CAGGCTTCAA TGGGTTTACT 
CCCCTGGTCA CCCTTCTCTT AAGACACATC ATTGAGGACC CCTGTACCCT TCGTCATACC 
ATGGAAAAGG TTGTTCGCTC AGCAGCTACA AGTGGAGCTG GTAGCACTAC CTCTGGTGTT 
GTGTCTGGCA GCCTCGGCTC TCGGGAGATC AACTACATCC TTCGTGTCCT TGGGCCAGCC 
GCATGCCGCA ATCCAGACAT ATTCACAGAA GTGGCCAACT GCTGTATCCG CATCGCCCTT 
CCTGCCCCTC GAGGCTCAGG AACTGCTTCA GATGATGAAT TTGAGAATCT TAGAATTAAA 
GGCCCTAATG CTGTACAGCT GGTGAAGACC ACCCCTTTGA AGCCCTCACC TCTGCCTGTC 
ATCCCTGATA CTATCAAGGA AGTGATCTAT GATATGCTGA ATGCTCTGGC TGCATACCAT 
GCTCCAGAGG AAGCAGATAA ATCTGATCCT AAACCTGGGG TTATGACCCA AGAGGTTGGC 
CAGCTCCTGC AAGACATGGG TGATGATGTA TACCAGCAGT ACCGGTCACT TACGCGTCAG 
AGCAGTGACT TTGATACGCA GTCAGGTTTT TCCATTAATA GTCAGGTCTT TGCTGCAGAT 
GGTGCCTCCA CTGAGACTTC CGCATCTGGG ACCTCCCAAG GAGAGGCTTC AACTCCAGAG 
GAGTCTCGAG ATGGGAAGAA AGATAAAGAA GGGGACCGGG CCTCTGAGGA AGGCAAACAG 
AAAGGCAAGG GCAGCAAACC TTTAATGCCT ACCTCCACTA TCCTTCGTCT TCTGGCAGAG 
TTGGTGAGGT CCTATGTTGG TATTGCTACC CTGATTGCCA ACTACAGCTA CACTGTGGGC 
CAGTCTGAAC TGATCAAAGA GGACTGCAGT GTGCTAGCTT TTGTTCTGGA CCACCTGCTC 
CCACATACCC AGAATGCAGA AGACAAGGAC ACCCCTGCCT TGGCCCGCCT GTTCCTCGCA 
AGCCTGGCTG CTGCAGGGAG TGGCACAGAT GCCCAGGTGG CCCTAGTGAA TGAAGTAAAA 
GCAGCCCTTG GACGGGCACT GGCTATGGCT GAGAGTACAG AGAAACATGC CAGGCTTCAG 
GCAGTGATGT GTATCATCAG TACTATCATG GAGTCCTGCC CCTCCACCTC CAGCTTCTAC 
AGCAGTGCCA CAGCGAAGAC CCAGCACAAT GGCATGAACA ACATCATTCG GCTTTTCCTG 
AAGAAGGGAC TGGTTAATGA CCTGGCCAGA GTACCTCACA GCTTAGACCT GTCCAGTCCC 
AACATGGCCA ACACAGTCAA TGCTGCTCTG AAGCCTTTGG AAACACTTTC CCGGATTGTG 
AACCAGCCCA GTAGCCTTTT TGGCAGCAAG AGTGCTTCTA GCAAGAACAA GTCTGAGCAG 
GATGCCCAAG GAGCCTCTCA AGATTCCAGT AGCAACCAGC AGGACCCAGG CGAGCCTGGG 
GAAGCAGAAG TGCAGGAGGA GGATCATGAT GTCACTCAGA CAGAGGTGGC AGATGGGGAT 
ATCATGGATG GGGAGGCTGA AACCGACTCA GTGGTGATTG CTGGGCAGCC TGAGGTGCTC 
AGTTCACAAG AGATGCAGGT TGAGAATGAG CTGGAGGACC TGATAGATGA GTTGCTTGAG 
AGGGATGGCG GATCTGGGAA CAGTACAATT ATAGTGAGCA GAAGTGGAGA GGATGAATCA 
CAAGAGGACG TGCTGATGGA TGAAGCTCCT TCCAACCTCA GCCAAGCTTC CACCTTGCAG 
GCCAACCGAG AAGATTCCAT GAATATCCTG GACCCTGAGG ATGAGGAGGA GCACACTCAG 
GAAGAGGACA GCAGTGGCAG TAACGAGGAT GAGGATGATA GTCAGGATGA AGAGGAGGAG 
GAGGAGGAAG ATGAGGAAGA TGATCAGGAG GATGATGAAG GTGAAGAGGG AGATGAAGAC 
GATGACGACG ATGGCTCTGA GATGGAATTG GATGAGGATT ATCCTGATAT GAACGCTTCT 
CCCTTGGTCC GATTTGAGCG CTTTGACCGG GAGGATGATC TCATCATTGA GTTTGACAAC 
ATGTTCTCCA GTGCTACAGA CATCCCCCCA TCCCCAGGAA ATATCCCTAC CACCCATCCA 
CTGATGGTGC GCCATGCAGA CCACAGTTCT CTGACACTGG GCAGTGGCTC TTCAACAACT 
CGTCTCACCC AGGGCATCGG GCGCAGTCAG AGGACCCTAA GGCAGCTGAC GGCCAATACT 
GGCCACACCA TTCATGTTCA CTACCCTGGG AATCGCCAGC CCAACCCTCC TCTTATACTG 
CAGAGGTTGC TTGGTCCCTC AGCTGCTGCT GACATCCTTC AGCTGAGCAG CAGCCTTCCC 
CTACAAAGCC GGGGTCGGGC CCGCCTCCTG GTAGGCAACG ATGACGTCCA CATCATCGCC 
CGTTCTGATG ATGAGCTGCT GGATGACTTT TTCCATGATC AGAGCACAGC TACCAGCCAA 
GCAGGAACCC TGTCCAGCAT CCCCACAGCC CTGACCCGCT GGACAGAAGA ATGCAAAGTT 
CTCGATGCTG AGAGCATGCA TGACTGTGTT TCAGTGGTTA AAGTGTCCAT TGTCAATCAC 
CTGGAATTCC TGAGGGATGA GGAGCTGGAA GAAAGGCGAG AGAAGCGCAG GAAACAACTG 
GCTGAGGAAG AAACAAAGAT AACTGATAAA GGCAAAGAAG ATAAGGAGAA CAGGGATCAG 
AGTGCACAGT GTACTGCATC TAAGTCAAAT GACTCCACTG AACAGAATCT CTCAGATGGG 
ACGCCTATGC CTGACAGCTA CCCAACAACC CCATCTTCAA CTGATGCAGC TACATCTGAG 
TCCAAGGAGA CCCTTGGCAC TCTGCAATCC TCACAACAGC AACCAACACT CCCAACCCCA 
CCAGCTTTGG GAGAGGTTCC TCAGGAGCTG CAGTCTCCAG CTGGAGAAGG GGGCAGCTCT 
ACACAGCTAT TGATGCCTGT AGAGCCAGAG GAATTGGGTC CCACAAGGCC AAGTGGGGAA 
GCAGAAACAA CTCAGATGGA GTTATCCCCA GCTCCCACTA TAACCTCACT TTCCCCAGAG 
AGAGCTGAGG ATTCTGATGC ACTGACGGCT GTCAGCAGTC AGCTAGAAGG CTCTCCTATG 
GATACAAGCA GCCTGGCTTC CTGTACCTTA GAGGAGGCTG TGGGTGACAC TTCAGCAGCT 
GGCAGTTCTG AGCAGCCCAG AGCAGGCAGC TCCACTCCTG GGGATGCCCC ACCAGCTGTG 
GCGGAAGTGC AAGGCAGGAG TGATGGGTCA GGGGAATCTG CCCAGCCACC TGAGGACAGC 
TCCCCACCTG CATCCTCTGA GAGCTCTTCC ACCAGAGATT CTGCCGTGGC CATTTCTGGA 
GCAGATTCCC GAGGAATCCT AGAAGAGCCG TTGCCTTCAA CAAGCAGTGA AGAAGAAGAT 
CCCCTTGCGG GTATCAGTCT CCCTGAAGGT GTGGACCCCT CTTTTCTGGC TGCCCTGCCT 
GATGACATCC GTCGGGAAGT TCTACAGAAC CAGCTAGGCA TTCGTCCACC AACCCGGACT 
GCCCCCTCCA CAAATAGCTC AGCGCCTGCA GTGGTGGGGA ATCCTGGTGT GACTGAAGTG 
AGCCCTGAGT TTCTGGCTGC CCTGCCTCCA GCCATTCAGG AGGAAGTACT GGCACAGCAG 
AGAGCTGAGC AGCAGCGACG AGAACTAGCA CAGAATGCCA GCTCAGACAC CCCTATGGAC 
CCTGTGACCT TCATCCAGAC TCTGCCCTCA GACCTGCGCC GTAGTGTCCT AGAGGATATG 
GAGGACAGTG TGTTAGCTGT GATGCCACCT GACATTGCAG CTGAGGCTCA AGCCCTGAGA 
CGAGAGCAAG AAGCCCGGCA GCGACAGCTC ATGCATGAGC GTCTGTTTGG GCACAGTAGC 
ACCTCCGCAC TCTCTGCTAT TCTCCGAAGC CCGGCTTTCA CCAGTCGCTT AAGTGGCAAC 
CGTGGGGTCC AGTATACTCG CCTTGCTGTG CAGAGAGGTG GCACCTTCCA GATGGGGGGT 
AGCAGCAGCC ATAACAGGCC TTCTGGCAGT AATGTAGATA CTCTCCTCCG CCTCCGAGGA 
CGGCTCCTTC TGGACCACGA AGCCCTTTCT TGTCTCTTGG TCCTACTTTT TGTGGATGAG 
CCAAAGCTCA ATACTAGCCG TCTACACCGA GTACTGAGAA ATCTCTGCTA CCATGCCCAG 
ACCCGCCACT GGGTCATCCG CAGTCTGCTC TCCATCTTGC AGCGCAGCAG TGAGAGTGAG 
CTATGCATTG AAACACCCAA ACTCACTACA AGTGAGGAAA AGGGCAAAAA GTCGAGCAAG 
AGCTGTGGGT CAAGTAGCCA TGAGAACCGT CCCCTGGACC TGCTACACAA GATGGAGTCA 
AAGAGCTCCA ACCAGCTTTC CTGGCTCTCA GTATCCATGG ATGCAGCCCT AGGCTGCAGG 
ACTAATATAT TTCAGATCCA GCGTTCAGGG GGGCGTAAAC ATACCGAGAA GCATGCAAGC 
GGTGGCTCCA CCGTCCACAT CCATCCCCAA GCTGCTCCTG TTGTCTGCAG ACACGTTTTG 
GATACACTCA TTCAATTGGC CAAGGTATTT CCCAGCCACT TCACACAGCA GCGGACCAAA 
GAAACAAACT GTGAGAGTGA TCGGGAAAGG GGCAATAAGG CCTGTAGCCC ATGCTCCTCA 
CAGTCCTCCA GCAGTGGCAT TTGCACAGAC TTCTGGGACT TATTGGTAAA ACTGGACAAC 
ATGAATGTCA GCCGGAAAGG CAAGAACTCC GTGAAGTCAG TGCCAGTGAG CGCTGGCGGT 
GAGGGGGAAA CCTCTCCATA CAGCCTCGAG GCCTCTCCAC TGGGGCAGCT CATGAACATG 
TTGTCACACC CAGTCATCCG CCGGAGCTCT CTCTTAACTG AGAAACTCCT CAGACTCCTT 
TCTCTCATCT CAATTGCTCT CCCAGAAAAC AAGGTGTCAG AAGCACAGGC TAATTCTGGC 
AGCGGTGCTT CCTCCACCAC CACTGCCACC TCAACCACAT CTACCACCAC CACCACTGCC 
GCCTCCACCA CGCCCACACC CCCTACTGCA CCCACCCCTG TCACTTCTGC TCCAGCCCTG 
GTTGCTGCCA CGGCTATTTC CACCATTGTC GTAGCTGCTT CGACCACAGT GACTACCCCC 
ACGACTGCTA CCACTACTGT TTCAATTTCT CCCACTACTA AGGGCAGCAA ATCTCCAGCG 
AAGGTGAGTG ATGGGGGCAG CAGCAGTACA GACTTTAAGA TGGTGTCCTC TGGCCTCACT 
GAAAACCAGC TACAGCTCTC TGTAGAGGTG TTGACATCCC ACTCTTGTTC TGAGGAAGGC 
TTAGAGGATG CAGCCAACGT ACTACTGCAG CTCTCCCGGG GGGACTCTGG GACCCGGGAC 
ACTGTTCTCA AGCTGCTACT GAATGGAGCC CGCCATCTGG GTTATACCCT TTGTAAACAA 
ATAGGTACCC TGCTGGCCGA GCTGCGGGAA TACAACCTCG AGCAGCAGCG GCGAGCCCAA 
TGTGAAACCC TCTCTCCTGA TGGCCTGCCT GAGGAGCAGC CACAGACCAC CAAGCTGAAG 
GGCAAAATGC AGAGCAGGTT TGACATGGCT GAGAATGTGG TAATTGTGGC ATCTCAGAAG 
CGACCTTTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA 
TCTACCCAGA AGTTCTTCTT GAGGGTACTA CAGGTCATCA TCCAGCTCCG GGACGACACG 
CGCCGGGCTA ACAAGAAAGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA 
GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA 
ATGGTACGTG AGGGTCAAAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCTAGC 
CAGTCAGAGG CGTCTGTCCG GAGGGAGGAA TCACCCATGG ATGTGGACCA GCCATCTCCC 
AGTGCTCAAG ATACTCAATC CATTGCCTCC GATGGAACCC CACAGGGGGA GAAGGAAAAG 
GAAGAAAGAC CACCTGAGTT ACCCCTGCTC AGCGAGCAGC TGAGTTTGGA CGAGCTGTGG 
GACATGCTTG GGGAGTGTCT AAAGGAACTA GAGGAATCCC ATGACCAGCA TGCGGTGCTA 
GTGCTACAGC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CCACAGAGCG GGAGAGCAAG 
CCTCCTGTCC GAGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCTCCTCCA 
CTCTCCCCTG CCCCCTTAAC CCCAGCCACG CCTTCCTCCC TTGACCCATT CTTCTCCCGG 
GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTCCTTCGC 
TTTGCAGAGA CTCACCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC GACCCACCTT 
GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT TGATGTCAAG 
CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTAGATGAGG GGCTCCGGAA AGAAGACATG 
GCTGTGCATG TCCGTCGTGA CCATGTGTTT GAAGACTCCT ATCGTGAGCT GCATCGCAAA 
TCCCCCGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT 
GCTGGTGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG 
TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGAGTCACCT ACACCATCAA TCCATCTTCC 
CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTCG GACGCATTGT GGCCAAAGCT 
GTATATGACA ACCGTCTTCT GGAGTGCTAC TTTACTCGAT CCTTTTACAA ACACATCTTG 
GGCAAGTCAG TCAGATATAC AGATATGGAG AGTGAAGATT ACCACTTCTA CCAAGGTCTG 
GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ACCTCACCTT CAGCACTGAG 
GTCCAAGAGT TTGGAGTTTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG 
GTAACAGAGG AGAATAAGAA GGAGTATGTA CACCTGGTAT GCCAGATGAG AATGACAGGA 
GCCATCCGCA AGCAGTTGGC GGCTTTCTTA GAAGGCTTCT ATGAGATCAT TCCAAAGCGC 
CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTTA TATCAGGACT GCCCACCATT 
GACATCGATG ATCTGAAATC CAACACTGAA TACCACAAGT ACCAGTCCAA CTCTATTCAG 
ATCCAGTGGT TCTGGAGAGC ATTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC 
CAGTTTGTCA CAGGTACTTC CAAGGTACCC CTGCAAGGCT TTGCTGCCCT CGAAGGCATG 
AATGGCATTC AGAAGTTTCA GATCCATCGA GATGACAGGT CCACAGATCG CCTGCCTTCA 
GCTCACACAT GTTTTAATCA GCTGGATCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC 
CACATGCTAC TGTTGGCTAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308090.1
CDS	270..13643
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_024452322.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452322.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAATCAGC AAACAGTATC 
TTGTATAATG GCTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACGGA TAAGCAGCTT 
ATGGAGATTA AAGCAGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT 
CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCA 
GTGCTTGTAA GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATACCCTCAC 
CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC 
GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGATA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT 
GAGCATGAAG TAGATTTGTG CCGAAAAGAA TGTCCGTTTG TGATCAAGCC AAAGATCCAG 
AGACCCAATA CTACACAAGA AGGAGAGGAA ATGGAAACTG ATATGGATGT TGCAGATGTG 
GCTATGGAAA GCAGTCCAGG CTCATCCATC TCTATGGAGC ACAGGCTGGA TGTTGAATTA 
AGGGCATCAG GTTCCAGCAG CAGCACTAAC ATCTCTTCTG GCCCCAGCCC TGGTCCCAGT 
CCCGGCCCCG GCACCGGCCC TGGCCCCGGC CCCGGCCCCG GCCCCGGCCC TGGCCCCGGC 
CCCGGCCCCG GTCCTGGTCC CGGCCCTGGC CCCGGCCCTG GCCCTGGCCC CCGTCCTGGA 
GTCCAGTGTA TTCCACAACG AGCAGCACTT CTGAAATCCA TGTTGAATTT CCTCAAGAAG 
GCCATCCAAG ACCCTGCTTT CTCAGATGGC ATACGACATG TGATGGATGG TTCTCTGCCT 
ACCTCCCTGA AACACATCAT CAGCAATGCA GAATACTATG GCCCATCACT CTTCCTCCTA 
GCTACTGAAG TGGTGACTGT GTTTGTATTT CAAGAACCAT CACTGCTCTC CTCACTCCAG 
GACAATGGAT TGACAGATGT CATGCTGCAT GCACTGCTTA TCAAAGATGT TCCTGCTACC 
CGTGAAGTCC TTGGCTCCCT CCCAAATGTA TTCAGTGCAC TCTGTTTGAA TGCCCGAGGT 
CTTCAGTCTT TTGTTCAGTG TCAGCCTTTT GAACGCCTCT TCAAAGTTCT TCTGTCTCCA 
GATTACCTCC CAGCCATGCG GAGGAGGAGA AGTTCTGATC CCCTTGGGGA TACTGCATCC 
AACCTGGGGA GTGCTGTCGA TGAGCTCATG AGACATCAGC CCACCCTTAA AACAGATGCA 
ACGACTGCCA TCATCAAGTT ACTTGAAGAA ATCTGTAATC TTGGAAGGGA CCCCAAATAC 
ATCTGTCAGA AGCCATCAAT CCAGAAGGCA GATGGCACTG CCACTGCTCC TCCCCCAAGG 
TCTAATCATG CCGCAGAAGA AGCCTCTAGT GAGGATGAGG AGGAAGAGGA AGTACAGGCC 
ATGCAGAGCT TTAATTCTAC CCAGCAAAAT GAAACTGAGC CTAATCAGCA GGTTGTTGGT 
ACAGAGGAAC GTATTCCTAT TCCCCTCATG GATTACATCC TTAATGTGAT GAAATTTGTG 
GAATCTATTC TGAGCAACAA TACAACAGAT GACCACTGCC AGGAATTTGT GAATCAGAAA 
GGACTGTTGC CTTTGGTTAC CATTTTGGGT CTTCCCAATC TGCCCATTGA CTTTCCCACA 
TCTGCTGCCT GTCAGGCTGT TGCAGGTGTC TGCAAATCCA TATTGACACT GTCACATGAA 
CCCAAAGTCC TTCAAGAGGG TCTCCTTCAG TTGGACTCCA TCCTCTCCTC CCTGGAGCCC 
TTACACCGCC CCATTGAATC CCCTGGGGGC TCAGTGTTGT TGCGAGAACT GGCTTGCGCA 
GGCAATGTTG CTGATGCTAC CCTCTCAGCC CAGGCCACAC CTCTGCTGCA TGCACTCACT 
GCTGCCCATG CCTACATCAT GATGTTTGTT CATACTTGCA GAGTTGGACA GAGTGAAATT 
CGTTCCATCT CCGTAAACCA GTGGGGCTCT CAATTGGGTC TGAGTGTTTT GAGCAAGCTG 
AGCCAGTTAT ACTGTTCCCT GGTGTGGGAA AGCACTGTCC TCCTCTCTCT GTGTACCCCA 
AACAGCCTAC CATCTGGGTG TGAATTTGGC CAGGCAGATA TGCAGAAACT GGTTCCAAAG 
GATGAGAAGG CAGGTACGAC CCAGGGCGGA AAAAGATCAG ATGGGGAACA GGATGGAGCA 
GCTGGAAGTA TGGATGCTTC TACCCAGGGC TTATTAGAAG GCATTGGGCT AGATGGTGAC 
ACATTGGCTC CCATGGAGAC AGATGAACCT ACTGCTTCAG ACTCTAAGGG CAAATCTAAA 
ATCACACCAG CAATGGCTGC CAGAATTAAG CAAATCAAGC CTTTGTTATC AGCTTCCTCC 
AGATTAGGCC GAGCACTTGC TGAGCTATTT GGACTTCTTG TTAAACTTTG TGTGGGATCT 
CCTGTCCGCC AGAGAAGGAG CCATCATGCT GCCAGCACCA CTACAGCACC GACACCTGCC 
GCGCGATCAA CAGCCTCAGC TCTCACTAAG CTCTTGACTA AGGGGTTATC TTGGCAGCCC 
CCACCATATA CACCTACTCC CCGATTCAGG CTGACATTCT TCATCTGTTC AGTTGGTTTC 
ACATCCCCAA TGCTGTTTGA TGAGAGGAAG TATCCCTACC ACCTCATGCT GCAAAAATTT 
CTCTGCTCCG GAGGCCACAA TGCTCTTTTT GAAACTTTCA ACTGGGCTCT GTCCATGGGA 
GGTAAAGTTC CTGTTTCTGA GGGATTGGAA CACTCAGACT TGCCTGATGG CACAGGAGAA 
TTCCTAGATG CCTGGCTTAT GCTGGTGGAG AAGATGGTGA ATCCCACCAC GGTGCTTGAA 
TCTCCACATT CGCTGCCTGC CAAATTGCCT GGAGGTGTCC AGAACTTTCC CCAGTTCAGT 
GCACTGCGCT TCCTTGTGGT AACTCAGAAA GCAGCCTTTA CTTGCATCAA AAACTTATGG 
AACCGGAAAC CCCTGAAGGT ATATGGTGGA CGAATGGCTG AATCGATGCT GGCCATTCTA 
TGCCACATCC TCCGAGGAGA ACCTGTGATT CGAGAGAGAC TAAGCAAGGA GAAGGAGGGG 
TCTCGAGGAG AAGAGGATAC AGGGCAAGAG GAAGGTGGCT CCCGCCGGGA ACCTCAAGTC 
AACCAGCAAC AACTGCAACA GCTCATGGAC ATGGGCTTCA CAAGGGAACA TGCAATGGAG 
GCACTGTTGA ACACCAGCAC CATGGAGCAG GCCACAGAGT ACCTTTTAAC CCACCCTCCT 
CCAATCATGG GAGGAGTTGT TCGGGATCTC AGCATGTCTG AAGAGGACCA GATGATGAGA 
GCAATTGCTA TGTCTCTGGG ACAGGATATT CCAATGGATC AAAGGGCAGA GTCACCTGAG 
GAAGTTGCTT GCCGGAAGGA GGAAGAGGAA CGGAAAGCTC GGGAAAAGCA GGAGGAGGAA 
GAGGCTAAAT GTCTAGAGAA GTTCCAGGAT GCTGACCCGT TGGAACAAGA TGAGCTCCAC 
ACTTTCACAG ATACTATGTT GCCAGGCTGC TTCCACCTTC TTGATGAGCT GCCAGACACA 
GTATACCGTG TGTGTGACCT GATCATGACA GCAATCAAAC GTAATGGAGC AGATTATCGT 
GACATGATTC TGAAGCAAGT AGTCAATCAG GTGTGGGAAG CTGCTGATGT ATTGATCAAA 
GCTGCTCTTC CCCTGACAAC AAGTGACACA AAAACCGTGT CAGAGTGGAT AAGTCAGATG 
GCCACACTGC CCCAGGCCTC CAATTTGGCT ACTAGAATCT TGCTTTTAAC GCTACTTTTT 
GAGGAGTTGA AGCTACCTTG TGCTTGGGTG GTTGAATCAA GTGGCATCCT TAATGTCCTA 
ATCAAACTCT TGGAAGTGGT TCAGCCCTGC CTCCAGGCAG CCAAGGAGCA GAAGGAAGTC 
CAGACCCCAA AGTGGATCAC ACCAGTGTTG CTCCTGATTG ATTTCTATGA AAAGACAGCC 
ATCTCCTCAA AAAGGAGAGC CCAGATGACT AAGTACCTGC AATCCAACAG CAACAACTGG 
CGCTGGTTTG ATGATCGCTC TGGGCGTTGG TGTAGTTACA GTGCAAGCAA CAATAGCACT 
ATTGATTCTG CCTGGAAATC TGGAGAGACA AGCGTGCGAT TCACTGCAGG CCGAAGAAGA 
TACACGGTCC AATTCACTAC AATGGTGCAG GTTAATGAGG AAACAGGGAA CCGACGCCCT 
GTGATGCTGA CTCTCCTCAG GGTACCTCGG CTGAATAAAA ATTCAAAAAA CAGCAATGGA 
CAGGAACTAG AGAAGACGCT GGAAGAAAGC AAAGAAATGG ATATCAAACG TAAAGAAAAT 
AAAGGCAATG ATACCCCTTT GGCCCTAGAG AGTACAAACA CTGAAAAGGA GACAAGCCTG 
GAGGAAACAA AAATCGGGGA GATCCTGATC CAGGGCTTGA CAGAAGATAT GGTGACTGTT 
TTAATCCGGG CCTGCGTGAG CATGCTGGGA GTCCCTGTGG ACCCAGATAC TTTGCATGCC 
ACCCTTCGTC TCTGTCTGAG GCTCACCCGG GACCACAAAT ATGCCATGAT GTTTGCAGAA 
CTGAAGAGTA CCCGCATGAT CTTGAATTTG ACCCAGAGCT CAGGCTTCAA TGGGTTTACT 
CCCCTGGTCA CCCTTCTCTT AAGACACATC ATTGAGGACC CCTGTACCCT TCGTCATACC 
ATGGAAAAGG TTGTTCGCTC AGCAGCTACA AGTGGAGCTG GTAGCACTAC CTCTGGTGTT 
GTGTCTGGCA GCCTCGGCTC TCGGGAGATC AACTACATCC TTCGTGTCCT TGGGCCAGCC 
GCATGCCGCA ATCCAGACAT ATTCACAGAA GTGGCCAACT GCTGTATCCG CATCGCCCTT 
CCTGCCCCTC GAGGCTCAGG AACTGCTTCA GATGATGAAT TTGAGAATCT TAGAATTAAA 
GGCCCTAATG CTGTACAGCT GGTGAAGACC ACCCCTTTGA AGCCCTCACC TCTGCCTGTC 
ATCCCTGATA CTATCAAGGA AGTGATCTAT GATATGCTGA ATGCTCTGGC TGCATACCAT 
GCTCCAGAGG AAGCAGATAA ATCTGATCCT AAACCTGGGG TTATGACCCA AGAGGTTGGC 
CAGCTCCTGC AAGACATGGG TGATGATGTA TACCAGCAGT ACCGGTCACT TACGCGTCAG 
AGCAGTGACT TTGATACGCA GTCAGGTTTT TCCATTAATA GTCAGGTCTT TGCTGCAGAT 
GGTGCCTCCA CTGAGACTTC CGCATCTGGG ACCTCCCAAG GAGAGGCTTC AACTCCAGAG 
GAGTCTCGAG ATGGGAAGAA AGATAAAGAA GGGGACCGGG CCTCTGAGGA AGGCAAACAG 
AAAGGCAAGG GCAGCAAACC TTTAATGCCT ACCTCCACTA TCCTTCGTCT TCTGGCAGAG 
TTGGTGAGGT CCTATGTTGG TATTGCTACC CTGATTGCCA ACTACAGCTA CACTGTGGGC 
CAGTCTGAAC TGATCAAAGA GGACTGCAGT GTGCTAGCTT TTGTTCTGGA CCACCTGCTC 
CCACATACCC AGAATGCAGA AGACAAGGAC ACCCCTGCCT TGGCCCGCCT GTTCCTCGCA 
AGCCTGGCTG CTGCAGGGAG TGGCACAGAT GCCCAGGTGG CCCTAGTGAA TGAAGTAAAA 
GCAGCCCTTG GACGGGCACT GGCTATGGCT GAGAGTACAG AGAAACATGC CAGGCTTCAG 
GCAGTGATGT GTATCATCAG TACTATCATG GAGTCCTGCC CCTCCACCTC CAGCTTCTAC 
AGCAGTGCCA CAGCGAAGAC CCAGCACAAT GGCATGAACA ACATCATTCG GCTTTTCCTG 
AAGAAGGGAC TGGTTAATGA CCTGGCCAGA GTACCTCACA GCTTAGACCT GTCCAGTCCC 
AACATGGCCA ACACAGTCAA TGCTGCTCTG AAGCCTTTGG AAACACTTTC CCGGATTGTG 
AACCAGCCCA GTAGCCTTTT TGGCAGCAAG AGTGCTTCTA GCAAGAACAA GTCTGAGCAG 
GATGCCCAAG GAGCCTCTCA AGATTCCAGT AGCAACCAGC AGGACCCAGG CGAGCCTGGG 
GAAGCAGAAG TGCAGGAGGA GGATCATGAT GTCACTCAGA CAGAGGTGGC AGATGGGGAT 
ATCATGGATG GGGAGGCTGA AACCGACTCA GTGGTGATTG CTGGGCAGCC TGAGGTGCTC 
AGTTCACAAG AGATGCAGGT TGAGAATGAG CTGGAGGACC TGATAGATGA GTTGCTTGAG 
AGGGATGGCG GATCTGGGAA CAGTACAATT ATAGTGAGCA GAAGTGGAGA GGATGAATCA 
CAAGAGGACG TGCTGATGGA TGAAGCTCCT TCCAACCTCA GCCAAGCTTC CACCTTGCAG 
GCCAACCGAG AAGATTCCAT GAATATCCTG GACCCTGAGG ATGAGGAGGA GCACACTCAG 
GAAGAGGACA GCAGTGGCAG TAACGAGGAT GAGGATGATA GTCAGGATGA AGAGGAGGAG 
GAGGAGGAAG ATGAGGAAGA TGATCAGGAG GATGATGAAG GTGAAGAGGG AGATGAAGAC 
GATGACGACG ATGGCTCTGA GATGGAATTG GATGAGGATT ATCCTGATAT GAACGCTTCT 
CCCTTGGTCC GATTTGAGCG CTTTGACCGG GAGGATGATC TCATCATTGA GTTTGACAAC 
ATGTTCTCCA GTGCTACAGA CATCCCCCCA TCCCCAGGAA ATATCCCTAC CACCCATCCA 
CTGATGGTGC GCCATGCAGA CCACAGTTCT CTGACACTGG GCAGTGGCTC TTCAACAACT 
CGTCTCACCC AGGGCATCGG GCGCAGTCAG AGGACCCTAA GGCAGCTGAC GGCCAATACT 
GGCCACACCA TTCATGTTCA CTACCCTGGG AATCGCCAGC CCAACCCTCC TCTTATACTG 
CAGAGGTTGC TTGGTCCCTC AGCTGCTGCT GACATCCTTC AGCTGAGCAG CAGCCTTCCC 
CTACAAAGCC GGGGTCGGGC CCGCCTCCTG GTAGGCAACG ATGACGTCCA CATCATCGCC 
CGTTCTGATG ATGAGCTGCT GGATGACTTT TTCCATGATC AGAGCACAGC TACCAGCCAA 
GCAGGAACCC TGTCCAGCAT CCCCACAGCC CTGACCCGCT GGACAGAAGA ATGCAAAGTT 
CTCGATGCTG AGAGCATGCA TGACTGTGTT TCAGTGGTTA AAGTGTCCAT TGTCAATCAC 
CTGGAATTCC TGAGGGATGA GGAGCTGGAA GAAAGGCGAG AGAAGCGCAG GAAACAACTG 
GCTGAGGAAG AAACAAAGAT AACTGATAAA GGCAAAGAAG ATAAGGAGAA CAGGGATCAG 
AGTGCACAGT GTACTGCATC TAAGTCAAAT GACTCCACTG AACAGAATCT CTCAGATGGG 
ACGCCTATGC CTGACAGCTA CCCAACAACC CCATCTTCAA CTGATGCAGC TACATCTGAG 
TCCAAGGAGA CCCTTGGCAC TCTGCAATCC TCACAACAGC AACCAACACT CCCAACCCCA 
CCAGCTTTGG GAGAGGTTCC TCAGGAGCTG CAGTCTCCAG CTGGAGAAGG GGGCAGCTCT 
ACACAGCTAT TGATGCCTGT AGAGCCAGAG GAATTGGGTC CCACAAGGCC AAGTGGGGAA 
GCAGAAACAA CTCAGATGGA GTTATCCCCA GCTCCCACTA TAACCTCACT TTCCCCAGAG 
AGAGCTGAGG ATTCTGATGC ACTGACGGCT GTCAGCAGTC AGCTAGAAGG CTCTCCTATG 
GATACAAGCA GCCTGGCTTC CTGTACCTTA GAGGAGGCTG TGGGTGACAC TTCAGCAGCT 
GGCAGTTCTG AGCAGCCCAG AGCAGGCAGC TCCACTCCTG GGGATGCCCC ACCAGCTGTG 
GCGGAAGTGC AAGGCAGGAG TGATGGGTCA GGGGAATCTG CCCAGCCACC TGAGGACAGC 
TCCCCACCTG CATCCTCTGA GAGCTCTTCC ACCAGAGATT CTGCCGTGGC CATTTCTGGA 
GCAGATTCCC GAGGAATCCT AGAAGAGCCG TTGCCTTCAA CAAGCAGTGA AGAAGAAGAT 
CCCCTTGCGG GTATCAGTCT CCCTGAAGGT GTGGACCCCT CTTTTCTGGC TGCCCTGCCT 
GATGACATCC GTCGGGAAGT TCTACAGAAC CAGCTAGGCA TTCGTCCACC AACCCGGACT 
GCCCCCTCCA CAAATAGCTC AGCGCCTGCA GTGGTGGGGA ATCCTGGTGT GACTGAAGTG 
AGCCCTGAGT TTCTGGCTGC CCTGCCTCCA GCCATTCAGG AGGAAGTACT GGCACAGCAG 
AGAGCTGAGC AGCAGCGACG AGAACTAGCA CAGAATGCCA GCTCAGACAC CCCTATGGAC 
CCTGTGACCT TCATCCAGAC TCTGCCCTCA GACCTGCGCC GTAGTGTCCT AGAGGATATG 
GAGGACAGTG TGTTAGCTGT GATGCCACCT GACATTGCAG CTGAGGCTCA AGCCCTGAGA 
CGAGAGCAAG AAGCCCGGCA GCGACAGCTC ATGCATGAGC GTCTGTTTGG GCACAGTAGC 
ACCTCCGCAC TCTCTGCTAT TCTCCGAAGC CCGGCTTTCA CCAGTCGCTT AAGTGGCAAC 
CGTGGGGTCC AGTATACTCG CCTTGCTGTG CAGAGAGGTG GCACCTTCCA GATGGGGGGT 
AGCAGCAGCC ATAACAGGCC TTCTGGCAGT AATGTAGATA CTCTCCTCCG CCTCCGAGGA 
CGGCTCCTTC TGGACCACGA AGCCCTTTCT TGTCTCTTGG TCCTACTTTT TGTGGATGAG 
CCAAAGCTCA ATACTAGCCG TCTACACCGA GTACTGAGAA ATCTCTGCTA CCATGCCCAG 
ACCCGCCACT GGGTCATCCG CAGTCTGCTC TCCATCTTGC AGCGCAGCAG TGAGAGTGAG 
CTATGCATTG AAACACCCAA ACTCACTACA AGTGAGGAAA AGGGCAAAAA GTCGAGCAAG 
AGCTGTGGGT CAAGTAGCCA TGAGAACCGT CCCCTGGACC TGCTACACAA GATGGAGTCA 
AAGAGCTCCA ACCAGCTTTC CTGGCTCTCA GTATCCATGG ATGCAGCCCT AGGCTGCAGG 
ACTAATATAT TTCAGATCCA GCGTTCAGGG GGGCGTAAAC ATACCGAGAA GCATGCAAGC 
GGTGGCTCCA CCGTCCACAT CCATCCCCAA GCTGCTCCTG TTGTCTGCAG ACACGTTTTG 
GATACACTCA TTCAATTGGC CAAGGTATTT CCCAGCCACT TCACACAGCA GCGGACCAAA 
GAAACAAACT GTGAGAGTGA TCGGGAAAGG GGCAATAAGG CCTGTAGCCC ATGCTCCTCA 
CAGTCCTCCA GCAGTGGCAT TTGCACAGAC TTCTGGGACT TATTGGTAAA ACTGGACAAC 
ATGAATGTCA GCCGGAAAGG CAAGAACTCC GTGAAGTCAG TGCCAGTGAG CGCTGGCGGT 
GAGGGGGAAA CCTCTCCATA CAGCCTCGAG GCCTCTCCAC TGGGGCAGCT CATGAACATG 
TTGTCACACC CAGTCATCCG CCGGAGCTCT CTCTTAACTG AGAAACTCCT CAGACTCCTT 
TCTCTCATCT CAATTGCTCT CCCAGAAAAC AAGGTGTCAG AAGCACAGGC TAATTCTGGC 
AGCGGTGCTT CCTCCACCAC CACTGCCACC TCAACCACAT CTACCACCAC CACCACTGCC 
GCCTCCACCA CGCCCACACC CCCTACTGCA CCCACCCCTG TCACTTCTGC TCCAGCCCTG 
GTTGCTGCCA CGGCTATTTC CACCATTGTC GTAGCTGCTT CGACCACAGT GACTACCCCC 
ACGACTGCTA CCACTACTGT TTCAATTTCT CCCACTACTA AGGGCAGCAA ATCTCCAGCG 
AAGGTGAGTG ATGGGGGCAG CAGCAGTACA GACTTTAAGA TGGTGTCCTC TGGCCTCACT 
GAAAACCAGC TACAGCTCTC TGTAGAGGTG TTGACATCCC ACTCTTGTTC TGAGGAAGGC 
TTAGAGGATG CAGCCAACGT ACTACTGCAG CTCTCCCGGG GGGACTCTGG GACCCGGGAC 
ACTGTTCTCA AGCTGCTACT GAATGGAGCC CGCCATCTGG GTTATACCCT TTGTAAACAA 
ATAGGTACCC TGCTGGCCGA GCTGCGGGAA TACAACCTCG AGCAGCAGCG GCGAGCCCAA 
TGTGAAACCC TCTCTCCTGA TGGCCTGCCT GAGGAGCAGC CACAGACCAC CAAGCTGAAG 
GGCAAAATGC AGAGCAGGTT TGACATGGCT GAGAATGTGG TAATTGTGGC ATCTCAGAAG 
CGACCTTTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA 
TCTACCCAGA AGTTCTTCTT GAGGGTACTA CAGGTCATCA TCCAGCTCCG GGACGACACG 
CGCCGGGCTA ACAAGAAAGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA 
GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA 
ATGGTACGTG AGGGTCAAAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCTAGC 
CAGTCAGAGG CGTCTGTCCG GAGGGAGGAA TCACCCATGG ATGTGGACCA GCCATCTCCC 
AGTGCTCAAG ATACTCAATC CATTGCCTCC GATGGAACCC CACAGGGGGA GAAGGAAAAG 
GAAGAAAGAC CACCTGAGTT ACCCCTGCTC AGCGAGCAGC TGAGTTTGGA CGAGCTGTGG 
GACATGCTTG GGGAGTGTCT AAAGGAACTA GAGGAATCCC ATGACCAGCA TGCGGTGCTA 
GTGCTACAGC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CCACAGAGCG GGAGAGCAAG 
CCTCCTGTCC GAGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCTCCTCCA 
CTCTCCCCTG CCCCCTTAAC CCCAGCCACG CCTTCCTCCC TTGACCCATT CTTCTCCCGG 
GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTCCTTCGC 
TTTGCAGAGA CTCACCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC GACCCACCTT 
GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT TGATGTCAAG 
CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTAGATGAGG GGCTCCGGAA AGAAGACATG 
GCTGTGCATG TCCGTCGTGA CCATGTGTTT GAAGACTCCT ATCGTGAGCT GCATCGCAAA 
TCCCCCGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT 
GCTGGTGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG 
TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGAGTCACCT ACACCATCAA TCCATCTTCC 
CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTCG GACGCATTGT GGCCAAAGCT 
GTATATGACA ACCGTCTTCT GGAGTGCTAC TTTACTCGAT CCTTTTACAA ACACATCTTG 
GGCAAGTCAG TCAGATATAC AGATATGGAG AGTGAAGATT ACCACTTCTA CCAAGGTCTG 
GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ACCTCACCTT CAGCACTGAG 
GTCCAAGAGT TTGGAGTTTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG 
GTAACAGAGG AGAATAAGAA GGAGTATGTA CACCTGGTAT GCCAGATGAG AATGACAGGA 
GCCATCCGCA AGCAGTTGGC GGCTTTCTTA GAAGGCTTCT ATGAGATCAT TCCAAAGCGC 
CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTTA TATCAGGACT GCCCACCATT 
GACATCGATG ATCTGAAATC CAACACTGAA TACCACAAGT ACCAGTCCAA CTCTATTCAG 
ATCCAGTGGT TCTGGAGAGC ATTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC 
CAGTTTGTCA CAGGTACTTC CAAGGTACCC CTGCAAGGCT TTGCTGCCCT CGAAGGCATG 
AATGGCATTC AGAAGTTTCA GATCCATCGA GATGACAGGT CCACAGATCG CCTGCCTTCA 
GCTCACACAT GTTTTAATCA GCTGGATCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC 
CACATGCTAC TGTTGGCTAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_011530751.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529053.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X16, mRNA.

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CloneID	OHu63653
Clone ID Related Accession (Same CDS sequence)	XM_011530747.1 , XM_024452322.1 , XM_011530751.2
Accession Version	XM_011530751.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530751.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAATCAGC AAACAGTATC 
TTGTATAATG GCTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACGGA TAAGCAGCTT 
ATGGAGATTA AAGCAGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT 
CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCA 
GTGCTTGTAA GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATACCCTCAC 
CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC 
GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGATA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT 
GAGCATGAAG TAGATTTGTG CCGAAAAGAA TGTCCGTTTG TGATCAAGCC AAAGATCCAG 
AGACCCAATA CTACACAAGA AGGAGAGGAA ATGGAAACTG ATATGGATGT TGCAGATGTG 
GCTATGGAAA GCAGTCCAGG CTCATCCATC TCTATGGAGC ACAGGCTGGA TGTTGAATTA 
AGGGCATCAG GTTCCAGCAG CAGCACTAAC ATCTCTTCTG GCCCCAGCCC TGGTCCCAGT 
CCCGGCCCCG GCACCGGCCC TGGCCCCGGC CCCGGCCCCG GCCCCGGCCC TGGCCCCGGC 
CCCGGCCCCG GTCCTGGTCC CGGCCCTGGC CCCGGCCCTG GCCCTGGCCC CCGTCCTGGA 
GTCCAGTGTA TTCCACAACG AGCAGCACTT CTGAAATCCA TGTTGAATTT CCTCAAGAAG 
GCCATCCAAG ACCCTGCTTT CTCAGATGGC ATACGACATG TGATGGATGG TTCTCTGCCT 
ACCTCCCTGA AACACATCAT CAGCAATGCA GAATACTATG GCCCATCACT CTTCCTCCTA 
GCTACTGAAG TGGTGACTGT GTTTGTATTT CAAGAACCAT CACTGCTCTC CTCACTCCAG 
GACAATGGAT TGACAGATGT CATGCTGCAT GCACTGCTTA TCAAAGATGT TCCTGCTACC 
CGTGAAGTCC TTGGCTCCCT CCCAAATGTA TTCAGTGCAC TCTGTTTGAA TGCCCGAGGT 
CTTCAGTCTT TTGTTCAGTG TCAGCCTTTT GAACGCCTCT TCAAAGTTCT TCTGTCTCCA 
GATTACCTCC CAGCCATGCG GAGGAGGAGA AGTTCTGATC CCCTTGGGGA TACTGCATCC 
AACCTGGGGA GTGCTGTCGA TGAGCTCATG AGACATCAGC CCACCCTTAA AACAGATGCA 
ACGACTGCCA TCATCAAGTT ACTTGAAGAA ATCTGTAATC TTGGAAGGGA CCCCAAATAC 
ATCTGTCAGA AGCCATCAAT CCAGAAGGCA GATGGCACTG CCACTGCTCC TCCCCCAAGG 
TCTAATCATG CCGCAGAAGA AGCCTCTAGT GAGGATGAGG AGGAAGAGGA AGTACAGGCC 
ATGCAGAGCT TTAATTCTAC CCAGCAAAAT GAAACTGAGC CTAATCAGCA GGTTGTTGGT 
ACAGAGGAAC GTATTCCTAT TCCCCTCATG GATTACATCC TTAATGTGAT GAAATTTGTG 
GAATCTATTC TGAGCAACAA TACAACAGAT GACCACTGCC AGGAATTTGT GAATCAGAAA 
GGACTGTTGC CTTTGGTTAC CATTTTGGGT CTTCCCAATC TGCCCATTGA CTTTCCCACA 
TCTGCTGCCT GTCAGGCTGT TGCAGGTGTC TGCAAATCCA TATTGACACT GTCACATGAA 
CCCAAAGTCC TTCAAGAGGG TCTCCTTCAG TTGGACTCCA TCCTCTCCTC CCTGGAGCCC 
TTACACCGCC CCATTGAATC CCCTGGGGGC TCAGTGTTGT TGCGAGAACT GGCTTGCGCA 
GGCAATGTTG CTGATGCTAC CCTCTCAGCC CAGGCCACAC CTCTGCTGCA TGCACTCACT 
GCTGCCCATG CCTACATCAT GATGTTTGTT CATACTTGCA GAGTTGGACA GAGTGAAATT 
CGTTCCATCT CCGTAAACCA GTGGGGCTCT CAATTGGGTC TGAGTGTTTT GAGCAAGCTG 
AGCCAGTTAT ACTGTTCCCT GGTGTGGGAA AGCACTGTCC TCCTCTCTCT GTGTACCCCA 
AACAGCCTAC CATCTGGGTG TGAATTTGGC CAGGCAGATA TGCAGAAACT GGTTCCAAAG 
GATGAGAAGG CAGGTACGAC CCAGGGCGGA AAAAGATCAG ATGGGGAACA GGATGGAGCA 
GCTGGAAGTA TGGATGCTTC TACCCAGGGC TTATTAGAAG GCATTGGGCT AGATGGTGAC 
ACATTGGCTC CCATGGAGAC AGATGAACCT ACTGCTTCAG ACTCTAAGGG CAAATCTAAA 
ATCACACCAG CAATGGCTGC CAGAATTAAG CAAATCAAGC CTTTGTTATC AGCTTCCTCC 
AGATTAGGCC GAGCACTTGC TGAGCTATTT GGACTTCTTG TTAAACTTTG TGTGGGATCT 
CCTGTCCGCC AGAGAAGGAG CCATCATGCT GCCAGCACCA CTACAGCACC GACACCTGCC 
GCGCGATCAA CAGCCTCAGC TCTCACTAAG CTCTTGACTA AGGGGTTATC TTGGCAGCCC 
CCACCATATA CACCTACTCC CCGATTCAGG CTGACATTCT TCATCTGTTC AGTTGGTTTC 
ACATCCCCAA TGCTGTTTGA TGAGAGGAAG TATCCCTACC ACCTCATGCT GCAAAAATTT 
CTCTGCTCCG GAGGCCACAA TGCTCTTTTT GAAACTTTCA ACTGGGCTCT GTCCATGGGA 
GGTAAAGTTC CTGTTTCTGA GGGATTGGAA CACTCAGACT TGCCTGATGG CACAGGAGAA 
TTCCTAGATG CCTGGCTTAT GCTGGTGGAG AAGATGGTGA ATCCCACCAC GGTGCTTGAA 
TCTCCACATT CGCTGCCTGC CAAATTGCCT GGAGGTGTCC AGAACTTTCC CCAGTTCAGT 
GCACTGCGCT TCCTTGTGGT AACTCAGAAA GCAGCCTTTA CTTGCATCAA AAACTTATGG 
AACCGGAAAC CCCTGAAGGT ATATGGTGGA CGAATGGCTG AATCGATGCT GGCCATTCTA 
TGCCACATCC TCCGAGGAGA ACCTGTGATT CGAGAGAGAC TAAGCAAGGA GAAGGAGGGG 
TCTCGAGGAG AAGAGGATAC AGGGCAAGAG GAAGGTGGCT CCCGCCGGGA ACCTCAAGTC 
AACCAGCAAC AACTGCAACA GCTCATGGAC ATGGGCTTCA CAAGGGAACA TGCAATGGAG 
GCACTGTTGA ACACCAGCAC CATGGAGCAG GCCACAGAGT ACCTTTTAAC CCACCCTCCT 
CCAATCATGG GAGGAGTTGT TCGGGATCTC AGCATGTCTG AAGAGGACCA GATGATGAGA 
GCAATTGCTA TGTCTCTGGG ACAGGATATT CCAATGGATC AAAGGGCAGA GTCACCTGAG 
GAAGTTGCTT GCCGGAAGGA GGAAGAGGAA CGGAAAGCTC GGGAAAAGCA GGAGGAGGAA 
GAGGCTAAAT GTCTAGAGAA GTTCCAGGAT GCTGACCCGT TGGAACAAGA TGAGCTCCAC 
ACTTTCACAG ATACTATGTT GCCAGGCTGC TTCCACCTTC TTGATGAGCT GCCAGACACA 
GTATACCGTG TGTGTGACCT GATCATGACA GCAATCAAAC GTAATGGAGC AGATTATCGT 
GACATGATTC TGAAGCAAGT AGTCAATCAG GTGTGGGAAG CTGCTGATGT ATTGATCAAA 
GCTGCTCTTC CCCTGACAAC AAGTGACACA AAAACCGTGT CAGAGTGGAT AAGTCAGATG 
GCCACACTGC CCCAGGCCTC CAATTTGGCT ACTAGAATCT TGCTTTTAAC GCTACTTTTT 
GAGGAGTTGA AGCTACCTTG TGCTTGGGTG GTTGAATCAA GTGGCATCCT TAATGTCCTA 
ATCAAACTCT TGGAAGTGGT TCAGCCCTGC CTCCAGGCAG CCAAGGAGCA GAAGGAAGTC 
CAGACCCCAA AGTGGATCAC ACCAGTGTTG CTCCTGATTG ATTTCTATGA AAAGACAGCC 
ATCTCCTCAA AAAGGAGAGC CCAGATGACT AAGTACCTGC AATCCAACAG CAACAACTGG 
CGCTGGTTTG ATGATCGCTC TGGGCGTTGG TGTAGTTACA GTGCAAGCAA CAATAGCACT 
ATTGATTCTG CCTGGAAATC TGGAGAGACA AGCGTGCGAT TCACTGCAGG CCGAAGAAGA 
TACACGGTCC AATTCACTAC AATGGTGCAG GTTAATGAGG AAACAGGGAA CCGACGCCCT 
GTGATGCTGA CTCTCCTCAG GGTACCTCGG CTGAATAAAA ATTCAAAAAA CAGCAATGGA 
CAGGAACTAG AGAAGACGCT GGAAGAAAGC AAAGAAATGG ATATCAAACG TAAAGAAAAT 
AAAGGCAATG ATACCCCTTT GGCCCTAGAG AGTACAAACA CTGAAAAGGA GACAAGCCTG 
GAGGAAACAA AAATCGGGGA GATCCTGATC CAGGGCTTGA CAGAAGATAT GGTGACTGTT 
TTAATCCGGG CCTGCGTGAG CATGCTGGGA GTCCCTGTGG ACCCAGATAC TTTGCATGCC 
ACCCTTCGTC TCTGTCTGAG GCTCACCCGG GACCACAAAT ATGCCATGAT GTTTGCAGAA 
CTGAAGAGTA CCCGCATGAT CTTGAATTTG ACCCAGAGCT CAGGCTTCAA TGGGTTTACT 
CCCCTGGTCA CCCTTCTCTT AAGACACATC ATTGAGGACC CCTGTACCCT TCGTCATACC 
ATGGAAAAGG TTGTTCGCTC AGCAGCTACA AGTGGAGCTG GTAGCACTAC CTCTGGTGTT 
GTGTCTGGCA GCCTCGGCTC TCGGGAGATC AACTACATCC TTCGTGTCCT TGGGCCAGCC 
GCATGCCGCA ATCCAGACAT ATTCACAGAA GTGGCCAACT GCTGTATCCG CATCGCCCTT 
CCTGCCCCTC GAGGCTCAGG AACTGCTTCA GATGATGAAT TTGAGAATCT TAGAATTAAA 
GGCCCTAATG CTGTACAGCT GGTGAAGACC ACCCCTTTGA AGCCCTCACC TCTGCCTGTC 
ATCCCTGATA CTATCAAGGA AGTGATCTAT GATATGCTGA ATGCTCTGGC TGCATACCAT 
GCTCCAGAGG AAGCAGATAA ATCTGATCCT AAACCTGGGG TTATGACCCA AGAGGTTGGC 
CAGCTCCTGC AAGACATGGG TGATGATGTA TACCAGCAGT ACCGGTCACT TACGCGTCAG 
AGCAGTGACT TTGATACGCA GTCAGGTTTT TCCATTAATA GTCAGGTCTT TGCTGCAGAT 
GGTGCCTCCA CTGAGACTTC CGCATCTGGG ACCTCCCAAG GAGAGGCTTC AACTCCAGAG 
GAGTCTCGAG ATGGGAAGAA AGATAAAGAA GGGGACCGGG CCTCTGAGGA AGGCAAACAG 
AAAGGCAAGG GCAGCAAACC TTTAATGCCT ACCTCCACTA TCCTTCGTCT TCTGGCAGAG 
TTGGTGAGGT CCTATGTTGG TATTGCTACC CTGATTGCCA ACTACAGCTA CACTGTGGGC 
CAGTCTGAAC TGATCAAAGA GGACTGCAGT GTGCTAGCTT TTGTTCTGGA CCACCTGCTC 
CCACATACCC AGAATGCAGA AGACAAGGAC ACCCCTGCCT TGGCCCGCCT GTTCCTCGCA 
AGCCTGGCTG CTGCAGGGAG TGGCACAGAT GCCCAGGTGG CCCTAGTGAA TGAAGTAAAA 
GCAGCCCTTG GACGGGCACT GGCTATGGCT GAGAGTACAG AGAAACATGC CAGGCTTCAG 
GCAGTGATGT GTATCATCAG TACTATCATG GAGTCCTGCC CCTCCACCTC CAGCTTCTAC 
AGCAGTGCCA CAGCGAAGAC CCAGCACAAT GGCATGAACA ACATCATTCG GCTTTTCCTG 
AAGAAGGGAC TGGTTAATGA CCTGGCCAGA GTACCTCACA GCTTAGACCT GTCCAGTCCC 
AACATGGCCA ACACAGTCAA TGCTGCTCTG AAGCCTTTGG AAACACTTTC CCGGATTGTG 
AACCAGCCCA GTAGCCTTTT TGGCAGCAAG AGTGCTTCTA GCAAGAACAA GTCTGAGCAG 
GATGCCCAAG GAGCCTCTCA AGATTCCAGT AGCAACCAGC AGGACCCAGG CGAGCCTGGG 
GAAGCAGAAG TGCAGGAGGA GGATCATGAT GTCACTCAGA CAGAGGTGGC AGATGGGGAT 
ATCATGGATG GGGAGGCTGA AACCGACTCA GTGGTGATTG CTGGGCAGCC TGAGGTGCTC 
AGTTCACAAG AGATGCAGGT TGAGAATGAG CTGGAGGACC TGATAGATGA GTTGCTTGAG 
AGGGATGGCG GATCTGGGAA CAGTACAATT ATAGTGAGCA GAAGTGGAGA GGATGAATCA 
CAAGAGGACG TGCTGATGGA TGAAGCTCCT TCCAACCTCA GCCAAGCTTC CACCTTGCAG 
GCCAACCGAG AAGATTCCAT GAATATCCTG GACCCTGAGG ATGAGGAGGA GCACACTCAG 
GAAGAGGACA GCAGTGGCAG TAACGAGGAT GAGGATGATA GTCAGGATGA AGAGGAGGAG 
GAGGAGGAAG ATGAGGAAGA TGATCAGGAG GATGATGAAG GTGAAGAGGG AGATGAAGAC 
GATGACGACG ATGGCTCTGA GATGGAATTG GATGAGGATT ATCCTGATAT GAACGCTTCT 
CCCTTGGTCC GATTTGAGCG CTTTGACCGG GAGGATGATC TCATCATTGA GTTTGACAAC 
ATGTTCTCCA GTGCTACAGA CATCCCCCCA TCCCCAGGAA ATATCCCTAC CACCCATCCA 
CTGATGGTGC GCCATGCAGA CCACAGTTCT CTGACACTGG GCAGTGGCTC TTCAACAACT 
CGTCTCACCC AGGGCATCGG GCGCAGTCAG AGGACCCTAA GGCAGCTGAC GGCCAATACT 
GGCCACACCA TTCATGTTCA CTACCCTGGG AATCGCCAGC CCAACCCTCC TCTTATACTG 
CAGAGGTTGC TTGGTCCCTC AGCTGCTGCT GACATCCTTC AGCTGAGCAG CAGCCTTCCC 
CTACAAAGCC GGGGTCGGGC CCGCCTCCTG GTAGGCAACG ATGACGTCCA CATCATCGCC 
CGTTCTGATG ATGAGCTGCT GGATGACTTT TTCCATGATC AGAGCACAGC TACCAGCCAA 
GCAGGAACCC TGTCCAGCAT CCCCACAGCC CTGACCCGCT GGACAGAAGA ATGCAAAGTT 
CTCGATGCTG AGAGCATGCA TGACTGTGTT TCAGTGGTTA AAGTGTCCAT TGTCAATCAC 
CTGGAATTCC TGAGGGATGA GGAGCTGGAA GAAAGGCGAG AGAAGCGCAG GAAACAACTG 
GCTGAGGAAG AAACAAAGAT AACTGATAAA GGCAAAGAAG ATAAGGAGAA CAGGGATCAG 
AGTGCACAGT GTACTGCATC TAAGTCAAAT GACTCCACTG AACAGAATCT CTCAGATGGG 
ACGCCTATGC CTGACAGCTA CCCAACAACC CCATCTTCAA CTGATGCAGC TACATCTGAG 
TCCAAGGAGA CCCTTGGCAC TCTGCAATCC TCACAACAGC AACCAACACT CCCAACCCCA 
CCAGCTTTGG GAGAGGTTCC TCAGGAGCTG CAGTCTCCAG CTGGAGAAGG GGGCAGCTCT 
ACACAGCTAT TGATGCCTGT AGAGCCAGAG GAATTGGGTC CCACAAGGCC AAGTGGGGAA 
GCAGAAACAA CTCAGATGGA GTTATCCCCA GCTCCCACTA TAACCTCACT TTCCCCAGAG 
AGAGCTGAGG ATTCTGATGC ACTGACGGCT GTCAGCAGTC AGCTAGAAGG CTCTCCTATG 
GATACAAGCA GCCTGGCTTC CTGTACCTTA GAGGAGGCTG TGGGTGACAC TTCAGCAGCT 
GGCAGTTCTG AGCAGCCCAG AGCAGGCAGC TCCACTCCTG GGGATGCCCC ACCAGCTGTG 
GCGGAAGTGC AAGGCAGGAG TGATGGGTCA GGGGAATCTG CCCAGCCACC TGAGGACAGC 
TCCCCACCTG CATCCTCTGA GAGCTCTTCC ACCAGAGATT CTGCCGTGGC CATTTCTGGA 
GCAGATTCCC GAGGAATCCT AGAAGAGCCG TTGCCTTCAA CAAGCAGTGA AGAAGAAGAT 
CCCCTTGCGG GTATCAGTCT CCCTGAAGGT GTGGACCCCT CTTTTCTGGC TGCCCTGCCT 
GATGACATCC GTCGGGAAGT TCTACAGAAC CAGCTAGGCA TTCGTCCACC AACCCGGACT 
GCCCCCTCCA CAAATAGCTC AGCGCCTGCA GTGGTGGGGA ATCCTGGTGT GACTGAAGTG 
AGCCCTGAGT TTCTGGCTGC CCTGCCTCCA GCCATTCAGG AGGAAGTACT GGCACAGCAG 
AGAGCTGAGC AGCAGCGACG AGAACTAGCA CAGAATGCCA GCTCAGACAC CCCTATGGAC 
CCTGTGACCT TCATCCAGAC TCTGCCCTCA GACCTGCGCC GTAGTGTCCT AGAGGATATG 
GAGGACAGTG TGTTAGCTGT GATGCCACCT GACATTGCAG CTGAGGCTCA AGCCCTGAGA 
CGAGAGCAAG AAGCCCGGCA GCGACAGCTC ATGCATGAGC GTCTGTTTGG GCACAGTAGC 
ACCTCCGCAC TCTCTGCTAT TCTCCGAAGC CCGGCTTTCA CCAGTCGCTT AAGTGGCAAC 
CGTGGGGTCC AGTATACTCG CCTTGCTGTG CAGAGAGGTG GCACCTTCCA GATGGGGGGT 
AGCAGCAGCC ATAACAGGCC TTCTGGCAGT AATGTAGATA CTCTCCTCCG CCTCCGAGGA 
CGGCTCCTTC TGGACCACGA AGCCCTTTCT TGTCTCTTGG TCCTACTTTT TGTGGATGAG 
CCAAAGCTCA ATACTAGCCG TCTACACCGA GTACTGAGAA ATCTCTGCTA CCATGCCCAG 
ACCCGCCACT GGGTCATCCG CAGTCTGCTC TCCATCTTGC AGCGCAGCAG TGAGAGTGAG 
CTATGCATTG AAACACCCAA ACTCACTACA AGTGAGGAAA AGGGCAAAAA GTCGAGCAAG 
AGCTGTGGGT CAAGTAGCCA TGAGAACCGT CCCCTGGACC TGCTACACAA GATGGAGTCA 
AAGAGCTCCA ACCAGCTTTC CTGGCTCTCA GTATCCATGG ATGCAGCCCT AGGCTGCAGG 
ACTAATATAT TTCAGATCCA GCGTTCAGGG GGGCGTAAAC ATACCGAGAA GCATGCAAGC 
GGTGGCTCCA CCGTCCACAT CCATCCCCAA GCTGCTCCTG TTGTCTGCAG ACACGTTTTG 
GATACACTCA TTCAATTGGC CAAGGTATTT CCCAGCCACT TCACACAGCA GCGGACCAAA 
GAAACAAACT GTGAGAGTGA TCGGGAAAGG GGCAATAAGG CCTGTAGCCC ATGCTCCTCA 
CAGTCCTCCA GCAGTGGCAT TTGCACAGAC TTCTGGGACT TATTGGTAAA ACTGGACAAC 
ATGAATGTCA GCCGGAAAGG CAAGAACTCC GTGAAGTCAG TGCCAGTGAG CGCTGGCGGT 
GAGGGGGAAA CCTCTCCATA CAGCCTCGAG GCCTCTCCAC TGGGGCAGCT CATGAACATG 
TTGTCACACC CAGTCATCCG CCGGAGCTCT CTCTTAACTG AGAAACTCCT CAGACTCCTT 
TCTCTCATCT CAATTGCTCT CCCAGAAAAC AAGGTGTCAG AAGCACAGGC TAATTCTGGC 
AGCGGTGCTT CCTCCACCAC CACTGCCACC TCAACCACAT CTACCACCAC CACCACTGCC 
GCCTCCACCA CGCCCACACC CCCTACTGCA CCCACCCCTG TCACTTCTGC TCCAGCCCTG 
GTTGCTGCCA CGGCTATTTC CACCATTGTC GTAGCTGCTT CGACCACAGT GACTACCCCC 
ACGACTGCTA CCACTACTGT TTCAATTTCT CCCACTACTA AGGGCAGCAA ATCTCCAGCG 
AAGGTGAGTG ATGGGGGCAG CAGCAGTACA GACTTTAAGA TGGTGTCCTC TGGCCTCACT 
GAAAACCAGC TACAGCTCTC TGTAGAGGTG TTGACATCCC ACTCTTGTTC TGAGGAAGGC 
TTAGAGGATG CAGCCAACGT ACTACTGCAG CTCTCCCGGG GGGACTCTGG GACCCGGGAC 
ACTGTTCTCA AGCTGCTACT GAATGGAGCC CGCCATCTGG GTTATACCCT TTGTAAACAA 
ATAGGTACCC TGCTGGCCGA GCTGCGGGAA TACAACCTCG AGCAGCAGCG GCGAGCCCAA 
TGTGAAACCC TCTCTCCTGA TGGCCTGCCT GAGGAGCAGC CACAGACCAC CAAGCTGAAG 
GGCAAAATGC AGAGCAGGTT TGACATGGCT GAGAATGTGG TAATTGTGGC ATCTCAGAAG 
CGACCTTTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA 
TCTACCCAGA AGTTCTTCTT GAGGGTACTA CAGGTCATCA TCCAGCTCCG GGACGACACG 
CGCCGGGCTA ACAAGAAAGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA 
GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA 
ATGGTACGTG AGGGTCAAAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCTAGC 
CAGTCAGAGG CGTCTGTCCG GAGGGAGGAA TCACCCATGG ATGTGGACCA GCCATCTCCC 
AGTGCTCAAG ATACTCAATC CATTGCCTCC GATGGAACCC CACAGGGGGA GAAGGAAAAG 
GAAGAAAGAC CACCTGAGTT ACCCCTGCTC AGCGAGCAGC TGAGTTTGGA CGAGCTGTGG 
GACATGCTTG GGGAGTGTCT AAAGGAACTA GAGGAATCCC ATGACCAGCA TGCGGTGCTA 
GTGCTACAGC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CCACAGAGCG GGAGAGCAAG 
CCTCCTGTCC GAGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCTCCTCCA 
CTCTCCCCTG CCCCCTTAAC CCCAGCCACG CCTTCCTCCC TTGACCCATT CTTCTCCCGG 
GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTCCTTCGC 
TTTGCAGAGA CTCACCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC GACCCACCTT 
GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT TGATGTCAAG 
CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTAGATGAGG GGCTCCGGAA AGAAGACATG 
GCTGTGCATG TCCGTCGTGA CCATGTGTTT GAAGACTCCT ATCGTGAGCT GCATCGCAAA 
TCCCCCGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT 
GCTGGTGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG 
TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGAGTCACCT ACACCATCAA TCCATCTTCC 
CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTCG GACGCATTGT GGCCAAAGCT 
GTATATGACA ACCGTCTTCT GGAGTGCTAC TTTACTCGAT CCTTTTACAA ACACATCTTG 
GGCAAGTCAG TCAGATATAC AGATATGGAG AGTGAAGATT ACCACTTCTA CCAAGGTCTG 
GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ACCTCACCTT CAGCACTGAG 
GTCCAAGAGT TTGGAGTTTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG 
GTAACAGAGG AGAATAAGAA GGAGTATGTA CACCTGGTAT GCCAGATGAG AATGACAGGA 
GCCATCCGCA AGCAGTTGGC GGCTTTCTTA GAAGGCTTCT ATGAGATCAT TCCAAAGCGC 
CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTTA TATCAGGACT GCCCACCATT 
GACATCGATG ATCTGAAATC CAACACTGAA TACCACAAGT ACCAGTCCAA CTCTATTCAG 
ATCCAGTGGT TCTGGAGAGC ATTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC 
CAGTTTGTCA CAGGTACTTC CAAGGTACCC CTGCAAGGCT TTGCTGCCCT CGAAGGCATG 
AATGGCATTC AGAAGTTTCA GATCCATCGA GATGACAGGT CCACAGATCG CCTGCCTTCA 
GCTCACACAT GTTTTAATCA GCTGGATCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC 
CACATGCTAC TGTTGGCTAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529053.1
CDS	266..13639
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_011530751.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530751.2

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAATCAGC AAACAGTATC 
TTGTATAATG GCTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACGGA TAAGCAGCTT 
ATGGAGATTA AAGCAGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT 
CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CCTACCATGG ATTTTTGCCA 
GTGCTTGTAA GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATACCCTCAC 
CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT 
GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC 
GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTTATCACC 
AACCTGGATA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT 
GAGCATGAAG TAGATTTGTG CCGAAAAGAA TGTCCGTTTG TGATCAAGCC AAAGATCCAG 
AGACCCAATA CTACACAAGA AGGAGAGGAA ATGGAAACTG ATATGGATGT TGCAGATGTG 
GCTATGGAAA GCAGTCCAGG CTCATCCATC TCTATGGAGC ACAGGCTGGA TGTTGAATTA 
AGGGCATCAG GTTCCAGCAG CAGCACTAAC ATCTCTTCTG GCCCCAGCCC TGGTCCCAGT 
CCCGGCCCCG GCACCGGCCC TGGCCCCGGC CCCGGCCCCG GCCCCGGCCC TGGCCCCGGC 
CCCGGCCCCG GTCCTGGTCC CGGCCCTGGC CCCGGCCCTG GCCCTGGCCC CCGTCCTGGA 
GTCCAGTGTA TTCCACAACG AGCAGCACTT CTGAAATCCA TGTTGAATTT CCTCAAGAAG 
GCCATCCAAG ACCCTGCTTT CTCAGATGGC ATACGACATG TGATGGATGG TTCTCTGCCT 
ACCTCCCTGA AACACATCAT CAGCAATGCA GAATACTATG GCCCATCACT CTTCCTCCTA 
GCTACTGAAG TGGTGACTGT GTTTGTATTT CAAGAACCAT CACTGCTCTC CTCACTCCAG 
GACAATGGAT TGACAGATGT CATGCTGCAT GCACTGCTTA TCAAAGATGT TCCTGCTACC 
CGTGAAGTCC TTGGCTCCCT CCCAAATGTA TTCAGTGCAC TCTGTTTGAA TGCCCGAGGT 
CTTCAGTCTT TTGTTCAGTG TCAGCCTTTT GAACGCCTCT TCAAAGTTCT TCTGTCTCCA 
GATTACCTCC CAGCCATGCG GAGGAGGAGA AGTTCTGATC CCCTTGGGGA TACTGCATCC 
AACCTGGGGA GTGCTGTCGA TGAGCTCATG AGACATCAGC CCACCCTTAA AACAGATGCA 
ACGACTGCCA TCATCAAGTT ACTTGAAGAA ATCTGTAATC TTGGAAGGGA CCCCAAATAC 
ATCTGTCAGA AGCCATCAAT CCAGAAGGCA GATGGCACTG CCACTGCTCC TCCCCCAAGG 
TCTAATCATG CCGCAGAAGA AGCCTCTAGT GAGGATGAGG AGGAAGAGGA AGTACAGGCC 
ATGCAGAGCT TTAATTCTAC CCAGCAAAAT GAAACTGAGC CTAATCAGCA GGTTGTTGGT 
ACAGAGGAAC GTATTCCTAT TCCCCTCATG GATTACATCC TTAATGTGAT GAAATTTGTG 
GAATCTATTC TGAGCAACAA TACAACAGAT GACCACTGCC AGGAATTTGT GAATCAGAAA 
GGACTGTTGC CTTTGGTTAC CATTTTGGGT CTTCCCAATC TGCCCATTGA CTTTCCCACA 
TCTGCTGCCT GTCAGGCTGT TGCAGGTGTC TGCAAATCCA TATTGACACT GTCACATGAA 
CCCAAAGTCC TTCAAGAGGG TCTCCTTCAG TTGGACTCCA TCCTCTCCTC CCTGGAGCCC 
TTACACCGCC CCATTGAATC CCCTGGGGGC TCAGTGTTGT TGCGAGAACT GGCTTGCGCA 
GGCAATGTTG CTGATGCTAC CCTCTCAGCC CAGGCCACAC CTCTGCTGCA TGCACTCACT 
GCTGCCCATG CCTACATCAT GATGTTTGTT CATACTTGCA GAGTTGGACA GAGTGAAATT 
CGTTCCATCT CCGTAAACCA GTGGGGCTCT CAATTGGGTC TGAGTGTTTT GAGCAAGCTG 
AGCCAGTTAT ACTGTTCCCT GGTGTGGGAA AGCACTGTCC TCCTCTCTCT GTGTACCCCA 
AACAGCCTAC CATCTGGGTG TGAATTTGGC CAGGCAGATA TGCAGAAACT GGTTCCAAAG 
GATGAGAAGG CAGGTACGAC CCAGGGCGGA AAAAGATCAG ATGGGGAACA GGATGGAGCA 
GCTGGAAGTA TGGATGCTTC TACCCAGGGC TTATTAGAAG GCATTGGGCT AGATGGTGAC 
ACATTGGCTC CCATGGAGAC AGATGAACCT ACTGCTTCAG ACTCTAAGGG CAAATCTAAA 
ATCACACCAG CAATGGCTGC CAGAATTAAG CAAATCAAGC CTTTGTTATC AGCTTCCTCC 
AGATTAGGCC GAGCACTTGC TGAGCTATTT GGACTTCTTG TTAAACTTTG TGTGGGATCT 
CCTGTCCGCC AGAGAAGGAG CCATCATGCT GCCAGCACCA CTACAGCACC GACACCTGCC 
GCGCGATCAA CAGCCTCAGC TCTCACTAAG CTCTTGACTA AGGGGTTATC TTGGCAGCCC 
CCACCATATA CACCTACTCC CCGATTCAGG CTGACATTCT TCATCTGTTC AGTTGGTTTC 
ACATCCCCAA TGCTGTTTGA TGAGAGGAAG TATCCCTACC ACCTCATGCT GCAAAAATTT 
CTCTGCTCCG GAGGCCACAA TGCTCTTTTT GAAACTTTCA ACTGGGCTCT GTCCATGGGA 
GGTAAAGTTC CTGTTTCTGA GGGATTGGAA CACTCAGACT TGCCTGATGG CACAGGAGAA 
TTCCTAGATG CCTGGCTTAT GCTGGTGGAG AAGATGGTGA ATCCCACCAC GGTGCTTGAA 
TCTCCACATT CGCTGCCTGC CAAATTGCCT GGAGGTGTCC AGAACTTTCC CCAGTTCAGT 
GCACTGCGCT TCCTTGTGGT AACTCAGAAA GCAGCCTTTA CTTGCATCAA AAACTTATGG 
AACCGGAAAC CCCTGAAGGT ATATGGTGGA CGAATGGCTG AATCGATGCT GGCCATTCTA 
TGCCACATCC TCCGAGGAGA ACCTGTGATT CGAGAGAGAC TAAGCAAGGA GAAGGAGGGG 
TCTCGAGGAG AAGAGGATAC AGGGCAAGAG GAAGGTGGCT CCCGCCGGGA ACCTCAAGTC 
AACCAGCAAC AACTGCAACA GCTCATGGAC ATGGGCTTCA CAAGGGAACA TGCAATGGAG 
GCACTGTTGA ACACCAGCAC CATGGAGCAG GCCACAGAGT ACCTTTTAAC CCACCCTCCT 
CCAATCATGG GAGGAGTTGT TCGGGATCTC AGCATGTCTG AAGAGGACCA GATGATGAGA 
GCAATTGCTA TGTCTCTGGG ACAGGATATT CCAATGGATC AAAGGGCAGA GTCACCTGAG 
GAAGTTGCTT GCCGGAAGGA GGAAGAGGAA CGGAAAGCTC GGGAAAAGCA GGAGGAGGAA 
GAGGCTAAAT GTCTAGAGAA GTTCCAGGAT GCTGACCCGT TGGAACAAGA TGAGCTCCAC 
ACTTTCACAG ATACTATGTT GCCAGGCTGC TTCCACCTTC TTGATGAGCT GCCAGACACA 
GTATACCGTG TGTGTGACCT GATCATGACA GCAATCAAAC GTAATGGAGC AGATTATCGT 
GACATGATTC TGAAGCAAGT AGTCAATCAG GTGTGGGAAG CTGCTGATGT ATTGATCAAA 
GCTGCTCTTC CCCTGACAAC AAGTGACACA AAAACCGTGT CAGAGTGGAT AAGTCAGATG 
GCCACACTGC CCCAGGCCTC CAATTTGGCT ACTAGAATCT TGCTTTTAAC GCTACTTTTT 
GAGGAGTTGA AGCTACCTTG TGCTTGGGTG GTTGAATCAA GTGGCATCCT TAATGTCCTA 
ATCAAACTCT TGGAAGTGGT TCAGCCCTGC CTCCAGGCAG CCAAGGAGCA GAAGGAAGTC 
CAGACCCCAA AGTGGATCAC ACCAGTGTTG CTCCTGATTG ATTTCTATGA AAAGACAGCC 
ATCTCCTCAA AAAGGAGAGC CCAGATGACT AAGTACCTGC AATCCAACAG CAACAACTGG 
CGCTGGTTTG ATGATCGCTC TGGGCGTTGG TGTAGTTACA GTGCAAGCAA CAATAGCACT 
ATTGATTCTG CCTGGAAATC TGGAGAGACA AGCGTGCGAT TCACTGCAGG CCGAAGAAGA 
TACACGGTCC AATTCACTAC AATGGTGCAG GTTAATGAGG AAACAGGGAA CCGACGCCCT 
GTGATGCTGA CTCTCCTCAG GGTACCTCGG CTGAATAAAA ATTCAAAAAA CAGCAATGGA 
CAGGAACTAG AGAAGACGCT GGAAGAAAGC AAAGAAATGG ATATCAAACG TAAAGAAAAT 
AAAGGCAATG ATACCCCTTT GGCCCTAGAG AGTACAAACA CTGAAAAGGA GACAAGCCTG 
GAGGAAACAA AAATCGGGGA GATCCTGATC CAGGGCTTGA CAGAAGATAT GGTGACTGTT 
TTAATCCGGG CCTGCGTGAG CATGCTGGGA GTCCCTGTGG ACCCAGATAC TTTGCATGCC 
ACCCTTCGTC TCTGTCTGAG GCTCACCCGG GACCACAAAT ATGCCATGAT GTTTGCAGAA 
CTGAAGAGTA CCCGCATGAT CTTGAATTTG ACCCAGAGCT CAGGCTTCAA TGGGTTTACT 
CCCCTGGTCA CCCTTCTCTT AAGACACATC ATTGAGGACC CCTGTACCCT TCGTCATACC 
ATGGAAAAGG TTGTTCGCTC AGCAGCTACA AGTGGAGCTG GTAGCACTAC CTCTGGTGTT 
GTGTCTGGCA GCCTCGGCTC TCGGGAGATC AACTACATCC TTCGTGTCCT TGGGCCAGCC 
GCATGCCGCA ATCCAGACAT ATTCACAGAA GTGGCCAACT GCTGTATCCG CATCGCCCTT 
CCTGCCCCTC GAGGCTCAGG AACTGCTTCA GATGATGAAT TTGAGAATCT TAGAATTAAA 
GGCCCTAATG CTGTACAGCT GGTGAAGACC ACCCCTTTGA AGCCCTCACC TCTGCCTGTC 
ATCCCTGATA CTATCAAGGA AGTGATCTAT GATATGCTGA ATGCTCTGGC TGCATACCAT 
GCTCCAGAGG AAGCAGATAA ATCTGATCCT AAACCTGGGG TTATGACCCA AGAGGTTGGC 
CAGCTCCTGC AAGACATGGG TGATGATGTA TACCAGCAGT ACCGGTCACT TACGCGTCAG 
AGCAGTGACT TTGATACGCA GTCAGGTTTT TCCATTAATA GTCAGGTCTT TGCTGCAGAT 
GGTGCCTCCA CTGAGACTTC CGCATCTGGG ACCTCCCAAG GAGAGGCTTC AACTCCAGAG 
GAGTCTCGAG ATGGGAAGAA AGATAAAGAA GGGGACCGGG CCTCTGAGGA AGGCAAACAG 
AAAGGCAAGG GCAGCAAACC TTTAATGCCT ACCTCCACTA TCCTTCGTCT TCTGGCAGAG 
TTGGTGAGGT CCTATGTTGG TATTGCTACC CTGATTGCCA ACTACAGCTA CACTGTGGGC 
CAGTCTGAAC TGATCAAAGA GGACTGCAGT GTGCTAGCTT TTGTTCTGGA CCACCTGCTC 
CCACATACCC AGAATGCAGA AGACAAGGAC ACCCCTGCCT TGGCCCGCCT GTTCCTCGCA 
AGCCTGGCTG CTGCAGGGAG TGGCACAGAT GCCCAGGTGG CCCTAGTGAA TGAAGTAAAA 
GCAGCCCTTG GACGGGCACT GGCTATGGCT GAGAGTACAG AGAAACATGC CAGGCTTCAG 
GCAGTGATGT GTATCATCAG TACTATCATG GAGTCCTGCC CCTCCACCTC CAGCTTCTAC 
AGCAGTGCCA CAGCGAAGAC CCAGCACAAT GGCATGAACA ACATCATTCG GCTTTTCCTG 
AAGAAGGGAC TGGTTAATGA CCTGGCCAGA GTACCTCACA GCTTAGACCT GTCCAGTCCC 
AACATGGCCA ACACAGTCAA TGCTGCTCTG AAGCCTTTGG AAACACTTTC CCGGATTGTG 
AACCAGCCCA GTAGCCTTTT TGGCAGCAAG AGTGCTTCTA GCAAGAACAA GTCTGAGCAG 
GATGCCCAAG GAGCCTCTCA AGATTCCAGT AGCAACCAGC AGGACCCAGG CGAGCCTGGG 
GAAGCAGAAG TGCAGGAGGA GGATCATGAT GTCACTCAGA CAGAGGTGGC AGATGGGGAT 
ATCATGGATG GGGAGGCTGA AACCGACTCA GTGGTGATTG CTGGGCAGCC TGAGGTGCTC 
AGTTCACAAG AGATGCAGGT TGAGAATGAG CTGGAGGACC TGATAGATGA GTTGCTTGAG 
AGGGATGGCG GATCTGGGAA CAGTACAATT ATAGTGAGCA GAAGTGGAGA GGATGAATCA 
CAAGAGGACG TGCTGATGGA TGAAGCTCCT TCCAACCTCA GCCAAGCTTC CACCTTGCAG 
GCCAACCGAG AAGATTCCAT GAATATCCTG GACCCTGAGG ATGAGGAGGA GCACACTCAG 
GAAGAGGACA GCAGTGGCAG TAACGAGGAT GAGGATGATA GTCAGGATGA AGAGGAGGAG 
GAGGAGGAAG ATGAGGAAGA TGATCAGGAG GATGATGAAG GTGAAGAGGG AGATGAAGAC 
GATGACGACG ATGGCTCTGA GATGGAATTG GATGAGGATT ATCCTGATAT GAACGCTTCT 
CCCTTGGTCC GATTTGAGCG CTTTGACCGG GAGGATGATC TCATCATTGA GTTTGACAAC 
ATGTTCTCCA GTGCTACAGA CATCCCCCCA TCCCCAGGAA ATATCCCTAC CACCCATCCA 
CTGATGGTGC GCCATGCAGA CCACAGTTCT CTGACACTGG GCAGTGGCTC TTCAACAACT 
CGTCTCACCC AGGGCATCGG GCGCAGTCAG AGGACCCTAA GGCAGCTGAC GGCCAATACT 
GGCCACACCA TTCATGTTCA CTACCCTGGG AATCGCCAGC CCAACCCTCC TCTTATACTG 
CAGAGGTTGC TTGGTCCCTC AGCTGCTGCT GACATCCTTC AGCTGAGCAG CAGCCTTCCC 
CTACAAAGCC GGGGTCGGGC CCGCCTCCTG GTAGGCAACG ATGACGTCCA CATCATCGCC 
CGTTCTGATG ATGAGCTGCT GGATGACTTT TTCCATGATC AGAGCACAGC TACCAGCCAA 
GCAGGAACCC TGTCCAGCAT CCCCACAGCC CTGACCCGCT GGACAGAAGA ATGCAAAGTT 
CTCGATGCTG AGAGCATGCA TGACTGTGTT TCAGTGGTTA AAGTGTCCAT TGTCAATCAC 
CTGGAATTCC TGAGGGATGA GGAGCTGGAA GAAAGGCGAG AGAAGCGCAG GAAACAACTG 
GCTGAGGAAG AAACAAAGAT AACTGATAAA GGCAAAGAAG ATAAGGAGAA CAGGGATCAG 
AGTGCACAGT GTACTGCATC TAAGTCAAAT GACTCCACTG AACAGAATCT CTCAGATGGG 
ACGCCTATGC CTGACAGCTA CCCAACAACC CCATCTTCAA CTGATGCAGC TACATCTGAG 
TCCAAGGAGA CCCTTGGCAC TCTGCAATCC TCACAACAGC AACCAACACT CCCAACCCCA 
CCAGCTTTGG GAGAGGTTCC TCAGGAGCTG CAGTCTCCAG CTGGAGAAGG GGGCAGCTCT 
ACACAGCTAT TGATGCCTGT AGAGCCAGAG GAATTGGGTC CCACAAGGCC AAGTGGGGAA 
GCAGAAACAA CTCAGATGGA GTTATCCCCA GCTCCCACTA TAACCTCACT TTCCCCAGAG 
AGAGCTGAGG ATTCTGATGC ACTGACGGCT GTCAGCAGTC AGCTAGAAGG CTCTCCTATG 
GATACAAGCA GCCTGGCTTC CTGTACCTTA GAGGAGGCTG TGGGTGACAC TTCAGCAGCT 
GGCAGTTCTG AGCAGCCCAG AGCAGGCAGC TCCACTCCTG GGGATGCCCC ACCAGCTGTG 
GCGGAAGTGC AAGGCAGGAG TGATGGGTCA GGGGAATCTG CCCAGCCACC TGAGGACAGC 
TCCCCACCTG CATCCTCTGA GAGCTCTTCC ACCAGAGATT CTGCCGTGGC CATTTCTGGA 
GCAGATTCCC GAGGAATCCT AGAAGAGCCG TTGCCTTCAA CAAGCAGTGA AGAAGAAGAT 
CCCCTTGCGG GTATCAGTCT CCCTGAAGGT GTGGACCCCT CTTTTCTGGC TGCCCTGCCT 
GATGACATCC GTCGGGAAGT TCTACAGAAC CAGCTAGGCA TTCGTCCACC AACCCGGACT 
GCCCCCTCCA CAAATAGCTC AGCGCCTGCA GTGGTGGGGA ATCCTGGTGT GACTGAAGTG 
AGCCCTGAGT TTCTGGCTGC CCTGCCTCCA GCCATTCAGG AGGAAGTACT GGCACAGCAG 
AGAGCTGAGC AGCAGCGACG AGAACTAGCA CAGAATGCCA GCTCAGACAC CCCTATGGAC 
CCTGTGACCT TCATCCAGAC TCTGCCCTCA GACCTGCGCC GTAGTGTCCT AGAGGATATG 
GAGGACAGTG TGTTAGCTGT GATGCCACCT GACATTGCAG CTGAGGCTCA AGCCCTGAGA 
CGAGAGCAAG AAGCCCGGCA GCGACAGCTC ATGCATGAGC GTCTGTTTGG GCACAGTAGC 
ACCTCCGCAC TCTCTGCTAT TCTCCGAAGC CCGGCTTTCA CCAGTCGCTT AAGTGGCAAC 
CGTGGGGTCC AGTATACTCG CCTTGCTGTG CAGAGAGGTG GCACCTTCCA GATGGGGGGT 
AGCAGCAGCC ATAACAGGCC TTCTGGCAGT AATGTAGATA CTCTCCTCCG CCTCCGAGGA 
CGGCTCCTTC TGGACCACGA AGCCCTTTCT TGTCTCTTGG TCCTACTTTT TGTGGATGAG 
CCAAAGCTCA ATACTAGCCG TCTACACCGA GTACTGAGAA ATCTCTGCTA CCATGCCCAG 
ACCCGCCACT GGGTCATCCG CAGTCTGCTC TCCATCTTGC AGCGCAGCAG TGAGAGTGAG 
CTATGCATTG AAACACCCAA ACTCACTACA AGTGAGGAAA AGGGCAAAAA GTCGAGCAAG 
AGCTGTGGGT CAAGTAGCCA TGAGAACCGT CCCCTGGACC TGCTACACAA GATGGAGTCA 
AAGAGCTCCA ACCAGCTTTC CTGGCTCTCA GTATCCATGG ATGCAGCCCT AGGCTGCAGG 
ACTAATATAT TTCAGATCCA GCGTTCAGGG GGGCGTAAAC ATACCGAGAA GCATGCAAGC 
GGTGGCTCCA CCGTCCACAT CCATCCCCAA GCTGCTCCTG TTGTCTGCAG ACACGTTTTG 
GATACACTCA TTCAATTGGC CAAGGTATTT CCCAGCCACT TCACACAGCA GCGGACCAAA 
GAAACAAACT GTGAGAGTGA TCGGGAAAGG GGCAATAAGG CCTGTAGCCC ATGCTCCTCA 
CAGTCCTCCA GCAGTGGCAT TTGCACAGAC TTCTGGGACT TATTGGTAAA ACTGGACAAC 
ATGAATGTCA GCCGGAAAGG CAAGAACTCC GTGAAGTCAG TGCCAGTGAG CGCTGGCGGT 
GAGGGGGAAA CCTCTCCATA CAGCCTCGAG GCCTCTCCAC TGGGGCAGCT CATGAACATG 
TTGTCACACC CAGTCATCCG CCGGAGCTCT CTCTTAACTG AGAAACTCCT CAGACTCCTT 
TCTCTCATCT CAATTGCTCT CCCAGAAAAC AAGGTGTCAG AAGCACAGGC TAATTCTGGC 
AGCGGTGCTT CCTCCACCAC CACTGCCACC TCAACCACAT CTACCACCAC CACCACTGCC 
GCCTCCACCA CGCCCACACC CCCTACTGCA CCCACCCCTG TCACTTCTGC TCCAGCCCTG 
GTTGCTGCCA CGGCTATTTC CACCATTGTC GTAGCTGCTT CGACCACAGT GACTACCCCC 
ACGACTGCTA CCACTACTGT TTCAATTTCT CCCACTACTA AGGGCAGCAA ATCTCCAGCG 
AAGGTGAGTG ATGGGGGCAG CAGCAGTACA GACTTTAAGA TGGTGTCCTC TGGCCTCACT 
GAAAACCAGC TACAGCTCTC TGTAGAGGTG TTGACATCCC ACTCTTGTTC TGAGGAAGGC 
TTAGAGGATG CAGCCAACGT ACTACTGCAG CTCTCCCGGG GGGACTCTGG GACCCGGGAC 
ACTGTTCTCA AGCTGCTACT GAATGGAGCC CGCCATCTGG GTTATACCCT TTGTAAACAA 
ATAGGTACCC TGCTGGCCGA GCTGCGGGAA TACAACCTCG AGCAGCAGCG GCGAGCCCAA 
TGTGAAACCC TCTCTCCTGA TGGCCTGCCT GAGGAGCAGC CACAGACCAC CAAGCTGAAG 
GGCAAAATGC AGAGCAGGTT TGACATGGCT GAGAATGTGG TAATTGTGGC ATCTCAGAAG 
CGACCTTTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA 
TCTACCCAGA AGTTCTTCTT GAGGGTACTA CAGGTCATCA TCCAGCTCCG GGACGACACG 
CGCCGGGCTA ACAAGAAAGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA 
GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA 
ATGGTACGTG AGGGTCAAAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCTAGC 
CAGTCAGAGG CGTCTGTCCG GAGGGAGGAA TCACCCATGG ATGTGGACCA GCCATCTCCC 
AGTGCTCAAG ATACTCAATC CATTGCCTCC GATGGAACCC CACAGGGGGA GAAGGAAAAG 
GAAGAAAGAC CACCTGAGTT ACCCCTGCTC AGCGAGCAGC TGAGTTTGGA CGAGCTGTGG 
GACATGCTTG GGGAGTGTCT AAAGGAACTA GAGGAATCCC ATGACCAGCA TGCGGTGCTA 
GTGCTACAGC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CCACAGAGCG GGAGAGCAAG 
CCTCCTGTCC GAGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCTCCTCCA 
CTCTCCCCTG CCCCCTTAAC CCCAGCCACG CCTTCCTCCC TTGACCCATT CTTCTCCCGG 
GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTCCTTCGC 
TTTGCAGAGA CTCACCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC GACCCACCTT 
GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT TGATGTCAAG 
CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTAGATGAGG GGCTCCGGAA AGAAGACATG 
GCTGTGCATG TCCGTCGTGA CCATGTGTTT GAAGACTCCT ATCGTGAGCT GCATCGCAAA 
TCCCCCGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT 
GCTGGTGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG 
TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGAGTCACCT ACACCATCAA TCCATCTTCC 
CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTCG GACGCATTGT GGCCAAAGCT 
GTATATGACA ACCGTCTTCT GGAGTGCTAC TTTACTCGAT CCTTTTACAA ACACATCTTG 
GGCAAGTCAG TCAGATATAC AGATATGGAG AGTGAAGATT ACCACTTCTA CCAAGGTCTG 
GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ACCTCACCTT CAGCACTGAG 
GTCCAAGAGT TTGGAGTTTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG 
GTAACAGAGG AGAATAAGAA GGAGTATGTA CACCTGGTAT GCCAGATGAG AATGACAGGA 
GCCATCCGCA AGCAGTTGGC GGCTTTCTTA GAAGGCTTCT ATGAGATCAT TCCAAAGCGC 
CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTTA TATCAGGACT GCCCACCATT 
GACATCGATG ATCTGAAATC CAACACTGAA TACCACAAGT ACCAGTCCAA CTCTATTCAG 
ATCCAGTGGT TCTGGAGAGC ATTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC 
CAGTTTGTCA CAGGTACTTC CAAGGTACCC CTGCAAGGCT TTGCTGCCCT CGAAGGCATG 
AATGGCATTC AGAAGTTTCA GATCCATCGA GATGACAGGT CCACAGATCG CCTGCCTTCA 
GCTCACACAT GTTTTAATCA GCTGGATCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC 
CACATGCTAC TGTTGGCTAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029191.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884680.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X1, mRNA.

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CloneID	OHu99112
Clone ID Related Accession (Same CDS sequence)	XM_017029191.1
Accession Version	XM_017029191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GTTGCAGATG TGGCTATGGA AAGCAGTCCA GGCTCATCCA TCTCTATGGA GCACAGGCTG 
GATGTTGAAT TAAGGGCATC AGGTTCCAGC AGCAGCACTA ACATCTCTTC TGGCCCCAGC 
CCTGGTCCCA GTCCCGGCCC CGGCACCGGC CCTGGCCCCG GCCCCGGCCC CGGCCCCGGC 
CCTGGCCCCG GCCCCGGCCC CGGTCCTGGT CCCGGCCCTG GCCCCGGCCC TGGCCCTGGC 
CCCCGTCCTG GAGTCCAGTG TATTCCACAA CGAGCAGCAC TTCTGAAATC CATGTTGAAT 
TTCCTCAAGA AGGCCATCCA AGACCCTGCT TTCTCAGATG GCATACGACA TGTGATGGAT 
GGTTCTCTGC CTACCTCCCT GAAACACATC ATCAGCAATG CAGAATACTA TGGCCCATCA 
CTCTTCCTCC TAGCTACTGA AGTGGTGACT GTGTTTGTAT TTCAAGAACC ATCACTGCTC 
TCCTCACTCC AGGACAATGG ATTGACAGAT GTCATGCTGC ATGCACTGCT TATCAAAGAT 
GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC CTCCCAAATG TATTCAGTGC ACTCTGTTTG 
AATGCCCGAG GTCTTCAGTC TTTTGTTCAG TGTCAGCCTT TTGAACGCCT CTTCAAAGTT 
CTTCTGTCTC CAGATTACCT CCCAGCCATG CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG 
GATACTGCAT CCAACCTGGG GAGTGCTGTC GATGAGCTCA TGAGACATCA GCCCACCCTT 
AAAACAGATG CAACGACTGC CATCATCAAG TTACTTGAAG AAATCTGTAA TCTTGGAAGG 
GACCCCAAAT ACATCTGTCA GAAGCCATCA ATCCAGAAGG CAGATGGCAC TGCCACTGCT 
CCTCCCCCAA GGTCTAATCA TGCCGCAGAA GAAGCCTCTA GTGAGGATGA GGAGGAAGAG 
GAAGTACAGG CCATGCAGAG CTTTAATTCT ACCCAGCAAA ATGAAACTGA GCCTAATCAG 
CAGGTTGTTG GTACAGAGGA ACGTATTCCT ATTCCCCTCA TGGATTACAT CCTTAATGTG 
ATGAAATTTG TGGAATCTAT TCTGAGCAAC AATACAACAG ATGACCACTG CCAGGAATTT 
GTGAATCAGA AAGGACTGTT GCCTTTGGTT ACCATTTTGG GTCTTCCCAA TCTGCCCATT 
GACTTTCCCA CATCTGCTGC CTGTCAGGCT GTTGCAGGTG TCTGCAAATC CATATTGACA 
CTGTCACATG AACCCAAAGT CCTTCAAGAG GGTCTCCTTC AGTTGGACTC CATCCTCTCC 
TCCCTGGAGC CCTTACACCG CCCCATTGAA TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA 
CTGGCTTGCG CAGGCAATGT TGCTGATGCT ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG 
CATGCACTCA CTGCTGCCCA TGCCTACATC ATGATGTTTG TTCATACTTG CAGAGTTGGA 
CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC CAGTGGGGCT CTCAATTGGG TCTGAGTGTT 
TTGAGCAAGC TGAGCCAGTT ATACTGTTCC CTGGTGTGGG AAAGCACTGT CCTCCTCTCT 
CTGTGTACCC CAAACAGCCT ACCATCTGGG TGTGAATTTG GCCAGGCAGA TATGCAGAAA 
CTGGTTCCAA AGGATGAGAA GGCAGGTACG ACCCAGGGCG GAAAAAGATC AGATGGGGAA 
CAGGATGGAG CAGCTGGAAG TATGGATGCT TCTACCCAGG GCTTATTAGA AGGCATTGGG 
CTAGATGGTG ACACATTGGC TCCCATGGAG ACAGATGAAC CTACTGCTTC AGACTCTAAG 
GGCAAATCTA AAATCACACC AGCAATGGCT GCCAGAATTA AGCAAATCAA GCCTTTGTTA 
TCAGCTTCCT CCAGATTAGG CCGAGCACTT GCTGAGCTAT TTGGACTTCT TGTTAAACTT 
TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG AGCCATCATG CTGCCAGCAC CACTACAGCA 
CCGACACCTG CCGCGCGATC AACAGCCTCA GCTCTCACTA AGCTCTTGAC TAAGGGGTTA 
TCTTGGCAGC CCCCACCATA TACACCTACT CCCCGATTCA GGCTGACATT CTTCATCTGT 
TCAGTTGGTT TCACATCCCC AATGCTGTTT GATGAGAGGA AGTATCCCTA CCACCTCATG 
CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC AATGCTCTTT TTGAAACTTT CAACTGGGCT 
CTGTCCATGG GAGGTAAAGT TCCTGTTTCT GAGGGATTGG AACACTCAGA CTTGCCTGAT 
GGCACAGGAG AATTCCTAGA TGCCTGGCTT ATGCTGGTGG AGAAGATGGT GAATCCCACC 
ACGGTGCTTG AATCTCCACA TTCGCTGCCT GCCAAATTGC CTGGAGGTGT CCAGAACTTT 
CCCCAGTTCA GTGCACTGCG CTTCCTTGTG GTAACTCAGA AAGCAGCCTT TACTTGCATC 
AAAAACTTAT GGAACCGGAA ACCCCTGAAG GTATATGGTG GACGAATGGC TGAATCGATG 
CTGGCCATTC TATGCCACAT CCTCCGAGGA GAACCTGTGA TTCGAGAGAG ACTAAGCAAG 
GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG 
GAACCTCAAG TCAACCAGCA ACAACTGCAA CAGCTCATGG ACATGGGCTT CACAAGGGAA 
CATGCAATGG AGGCACTGTT GAACACCAGC ACCATGGAGC AGGCCACAGA GTACCTTTTA 
ACCCACCCTC CTCCAATCAT GGGAGGAGTT GTTCGGGATC TCAGCATGTC TGAAGAGGAC 
CAGATGATGA GAGCAATTGC TATGTCTCTG GGACAGGATA TTCCAATGGA TCAAAGGGCA 
GAGTCACCTG AGGAAGTTGC TTGCCGGAAG GAGGAAGAGG AACGGAAAGC TCGGGAAAAG 
CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG AAGTTCCAGG ATGCTGACCC GTTGGAACAA 
GATGAGCTCC ACACTTTCAC AGATACTATG TTGCCAGGCT GCTTCCACCT TCTTGATGAG 
CTGCCAGACA CAGTATACCG TGTGTGTGAC CTGATCATGA CAGCAATCAA ACGTAATGGA 
GCAGATTATC GTGACATGAT TCTGAAGCAA GTAGTCAATC AGGTGTGGGA AGCTGCTGAT 
GTATTGATCA AAGCTGCTCT TCCCCTGACA ACAAGTGACA CAAAAACCGT GTCAGAGTGG 
ATAAGTCAGA TGGCCACACT GCCCCAGGCC TCCAATTTGG CTACTAGAAT CTTGCTTTTA 
ACGCTACTTT TTGAGGAGTT GAAGCTACCT TGTGCTTGGG TGGTTGAATC AAGTGGCATC 
CTTAATGTCC TAATCAAACT CTTGGAAGTG GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG 
CAGAAGGAAG TCCAGACCCC AAAGTGGATC ACACCAGTGT TGCTCCTGAT TGATTTCTAT 
GAAAAGACAG CCATCTCCTC AAAAAGGAGA GCCCAGATGA CTAAGTACCT GCAATCCAAC 
AGCAACAACT GGCGCTGGTT TGATGATCGC TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC 
AACAATAGCA CTATTGATTC TGCCTGGAAA TCTGGAGAGA CAAGCGTGCG ATTCACTGCA 
GGCCGAAGAA GATACACGGT CCAATTCACT ACAATGGTGC AGGTTAATGA GGAAACAGGG 
AACCGACGCC CTGTGATGCT GACTCTCCTC AGGGTACCTC GGCTGAATAA AAATTCAAAA 
AACAGCAATG GACAGGAACT AGAGAAGACG CTGGAAGAAA GCAAAGAAAT GGATATCAAA 
CGTAAAGAAA ATAAAGGCAA TGATACCCCT TTGGCCCTAG AGAGTACAAA CACTGAAAAG 
GAGACAAGCC TGGAGGAAAC AAAAATCGGG GAGATCCTGA TCCAGGGCTT GACAGAAGAT 
ATGGTGACTG TTTTAATCCG GGCCTGCGTG AGCATGCTGG GAGTCCCTGT GGACCCAGAT 
ACTTTGCATG CCACCCTTCG TCTCTGTCTG AGGCTCACCC GGGACCACAA ATATGCCATG 
ATGTTTGCAG AACTGAAGAG TACCCGCATG ATCTTGAATT TGACCCAGAG CTCAGGCTTC 
AATGGGTTTA CTCCCCTGGT CACCCTTCTC TTAAGACACA TCATTGAGGA CCCCTGTACC 
CTTCGTCATA CCATGGAAAA GGTTGTTCGC TCAGCAGCTA CAAGTGGAGC TGGTAGCACT 
ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC TCTCGGGAGA TCAACTACAT CCTTCGTGTC 
CTTGGGCCAG CCGCATGCCG CAATCCAGAC ATATTCACAG AAGTGGCCAA CTGCTGTATC 
CGCATCGCCC TTCCTGCCCC TCGAGGCTCA GGAACTGCTT CAGATGATGA ATTTGAGAAT 
CTTAGAATTA AAGGCCCTAA TGCTGTACAG CTGGTGAAGA CCACCCCTTT GAAGCCCTCA 
CCTCTGCCTG TCATCCCTGA TACTATCAAG GAAGTGATCT ATGATATGCT GAATGCTCTG 
GCTGCATACC ATGCTCCAGA GGAAGCAGAT AAATCTGATC CTAAACCTGG GGTTATGACC 
CAAGAGGTTG GCCAGCTCCT GCAAGACATG GGTGATGATG TATACCAGCA GTACCGGTCA 
CTTACGCGTC AGAGCAGTGA CTTTGATACG CAGTCAGGTT TTTCCATTAA TAGTCAGGTC 
TTTGCTGCAG ATGGTGCCTC CACTGAGACT TCCGCATCTG GGACCTCCCA AGGAGAGGCT 
TCAACTCCAG AGGAGTCTCG AGATGGGAAG AAAGATAAAG AAGGGGACCG GGCCTCTGAG 
GAAGGCAAAC AGAAAGGCAA GGGCAGCAAA CCTTTAATGC CTACCTCCAC TATCCTTCGT 
CTTCTGGCAG AGTTGGTGAG GTCCTATGTT GGTATTGCTA CCCTGATTGC CAACTACAGC 
TACACTGTGG GCCAGTCTGA ACTGATCAAA GAGGACTGCA GTGTGCTAGC TTTTGTTCTG 
GACCACCTGC TCCCACATAC CCAGAATGCA GAAGACAAGG ACACCCCTGC CTTGGCCCGC 
CTGTTCCTCG CAAGCCTGGC TGCTGCAGGG AGTGGCACAG ATGCCCAGGT GGCCCTAGTG 
AATGAAGTAA AAGCAGCCCT TGGACGGGCA CTGGCTATGG CTGAGAGTAC AGAGAAACAT 
GCCAGGCTTC AGGCAGTGAT GTGTATCATC AGTACTATCA TGGAGTCCTG CCCCTCCACC 
TCCAGCTTCT ACAGCAGTGC CACAGCGAAG ACCCAGCACA ATGGCATGAA CAACATCATT 
CGGCTTTTCC TGAAGAAGGG ACTGGTTAAT GACCTGGCCA GAGTACCTCA CAGCTTAGAC 
CTGTCCAGTC CCAACATGGC CAACACAGTC AATGCTGCTC TGAAGCCTTT GGAAACACTT 
TCCCGGATTG TGAACCAGCC CAGTAGCCTT TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC 
AAGTCTGAGC AGGATGCCCA AGGAGCCTCT CAAGATTCCA GTAGCAACCA GCAGGACCCA 
GGCGAGCCTG GGGAAGCAGA AGTGCAGGAG GAGGATCATG ATGTCACTCA GACAGAGGTG 
GCAGATGGGG ATATCATGGA TGGGGAGGCT GAAACCGACT CAGTGGTGAT TGCTGGGCAG 
CCTGAGGTGC TCAGTTCACA AGAGATGCAG GTTGAGAATG AGCTGGAGGA CCTGATAGAT 
GAGTTGCTTG AGAGGGATGG CGGATCTGGG AACAGTACAA TTATAGTGAG CAGAAGTGGA 
GAGGATGAAT CACAAGAGGA CGTGCTGATG GATGAAGCTC CTTCCAACCT CAGCCAAGCT 
TCCACCTTGC AGGCCAACCG AGAAGATTCC ATGAATATCC TGGACCCTGA GGATGAGGAG 
GAGCACACTC AGGAAGAGGA CAGCAGTGGC AGTAACGAGG ATGAGGATGA TAGTCAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGAGGAA GATGATCAGG AGGATGATGA AGGTGAAGAG 
GGAGATGAAG ACGATGACGA CGATGGCTCT GAGATGGAAT TGGATGAGGA TTATCCTGAT 
ATGAACGCTT CTCCCTTGGT CCGATTTGAG CGCTTTGACC GGGAGGATGA TCTCATCATT 
GAGTTTGACA ACATGTTCTC CAGTGCTACA GACATCCCCC CATCCCCAGG AAATATCCCT 
ACCACCCATC CACTGATGGT GCGCCATGCA GACCACAGTT CTCTGACACT GGGCAGTGGC 
TCTTCAACAA CTCGTCTCAC CCAGGGCATC GGGCGCAGTC AGAGGACCCT AAGGCAGCTG 
ACGGCCAATA CTGGCCACAC CATTCATGTT CACTACCCTG GGAATCGCCA GCCCAACCCT 
CCTCTTATAC TGCAGAGGTT GCTTGGTCCC TCAGCTGCTG CTGACATCCT TCAGCTGAGC 
AGCAGCCTTC CCCTACAAAG CCGGGGTCGG GCCCGCCTCC TGGTAGGCAA CGATGACGTC 
CACATCATCG CCCGTTCTGA TGATGAGCTG CTGGATGACT TTTTCCATGA TCAGAGCACA 
GCTACCAGCC AAGCAGGAAC CCTGTCCAGC ATCCCCACAG CCCTGACCCG CTGGACAGAA 
GAATGCAAAG TTCTCGATGC TGAGAGCATG CATGACTGTG TTTCAGTGGT TAAAGTGTCC 
ATTGTCAATC ACCTGGAATT CCTGAGGGAT GAGGAGCTGG AAGAAAGGCG AGAGAAGCGC 
AGGAAACAAC TGGCTGAGGA AGAAACAAAG ATAACTGATA AAGGCAAAGA AGATAAGGAG 
AACAGGGATC AGAGTGCACA GTGTACTGCA TCTAAGTCAA ATGACTCCAC TGAACAGAAT 
CTCTCAGATG GGACGCCTAT GCCTGACAGC TACCCAACAA CCCCATCTTC AACTGATGCA 
GCTACATCTG AGTCCAAGGA GACCCTTGGC ACTCTGCAAT CCTCACAACA GCAACCAACA 
CTCCCAACCC CACCAGCTTT GGGAGAGGTT CCTCAGGAGC TGCAGTCTCC AGCTGGAGAA 
GGGGGCAGCT CTACACAGCT ATTGATGCCT GTAGAGCCAG AGGAATTGGG TCCCACAAGG 
CCAAGTGGGG AAGCAGAAAC AACTCAGATG GAGTTATCCC CAGCTCCCAC TATAACCTCA 
CTTTCCCCAG AGAGAGCTGA GGATTCTGAT GCACTGACGG CTGTCAGCAG TCAGCTAGAA 
GGCTCTCCTA TGGATACAAG CAGCCTGGCT TCCTGTACCT TAGAGGAGGC TGTGGGTGAC 
ACTTCAGCAG CTGGCAGTTC TGAGCAGCCC AGAGCAGGCA GCTCCACTCC TGGGGATGCC 
CCACCAGCTG TGGCGGAAGT GCAAGGCAGG AGTGATGGGT CAGGGGAATC TGCCCAGCCA 
CCTGAGGACA GCTCCCCACC TGCATCCTCT GAGAGCTCTT CCACCAGAGA TTCTGCCGTG 
GCCATTTCTG GAGCAGATTC CCGAGGAATC CTAGAAGAGC CGTTGCCTTC AACAAGCAGT 
GAAGAAGAAG ATCCCCTTGC GGGTATCAGT CTCCCTGAAG GTGTGGACCC CTCTTTTCTG 
GCTGCCCTGC CTGATGACAT CCGTCGGGAA GTTCTACAGA ACCAGCTAGG CATTCGTCCA 
CCAACCCGGA CTGCCCCCTC CACAAATAGC TCAGCGCCTG CAGTGGTGGG GAATCCTGGT 
GTGACTGAAG TGAGCCCTGA GTTTCTGGCT GCCCTGCCTC CAGCCATTCA GGAGGAAGTA 
CTGGCACAGC AGAGAGCTGA GCAGCAGCGA CGAGAACTAG CACAGAATGC CAGCTCAGAC 
ACCCCTATGG ACCCTGTGAC CTTCATCCAG ACTCTGCCCT CAGACCTGCG CCGTAGTGTC 
CTAGAGGATA TGGAGGACAG TGTGTTAGCT GTGATGCCAC CTGACATTGC AGCTGAGGCT 
CAAGCCCTGA GACGAGAGCA AGAAGCCCGG CAGCGACAGC TCATGCATGA GCGTCTGTTT 
GGGCACAGTA GCACCTCCGC ACTCTCTGCT ATTCTCCGAA GCCCGGCTTT CACCAGTCGC 
TTAAGTGGCA ACCGTGGGGT CCAGTATACT CGCCTTGCTG TGCAGAGAGG TGGCACCTTC 
CAGATGGGGG GTAGCAGCAG CCATAACAGG CCTTCTGGCA GTAATGTAGA TACTCTCCTC 
CGCCTCCGAG GACGGCTCCT TCTGGACCAC GAAGCCCTTT CTTGTCTCTT GGTCCTACTT 
TTTGTGGATG AGCCAAAGCT CAATACTAGC CGTCTACACC GAGTACTGAG AAATCTCTGC 
TACCATGCCC AGACCCGCCA CTGGGTCATC CGCAGTCTGC TCTCCATCTT GCAGCGCAGC 
AGTGAGAGTG AGCTATGCAT TGAAACACCC AAACTCACTA CAAGTGAGGA AAAGGGCAAA 
AAGTCGAGCA AGAGCTGTGG GTCAAGTAGC CATGAGAACC GTCCCCTGGA CCTGCTACAC 
AAGATGGAGT CAAAGAGCTC CAACCAGCTT TCCTGGCTCT CAGTATCCAT GGATGCAGCC 
CTAGGCTGCA GGACTAATAT ATTTCAGATC CAGCGTTCAG GGGGGCGTAA ACATACCGAG 
AAGCATGCAA GCGGTGGCTC CACCGTCCAC ATCCATCCCC AAGCTGCTCC TGTTGTCTGC 
AGACACGTTT TGGATACACT CATTCAATTG GCCAAGGTAT TTCCCAGCCA CTTCACACAG 
CAGCGGACCA AAGAAACAAA CTGTGAGAGT GATCGGGAAA GGGGCAATAA GGCCTGTAGC 
CCATGCTCCT CACAGTCCTC CAGCAGTGGC ATTTGCACAG ACTTCTGGGA CTTATTGGTA 
AAACTGGACA ACATGAATGT CAGCCGGAAA GGCAAGAACT CCGTGAAGTC AGTGCCAGTG 
AGCGCTGGCG GTGAGGGGGA AACCTCTCCA TACAGCCTCG AGGCCTCTCC ACTGGGGCAG 
CTCATGAACA TGTTGTCACA CCCAGTCATC CGCCGGAGCT CTCTCTTAAC TGAGAAACTC 
CTCAGACTCC TTTCTCTCAT CTCAATTGCT CTCCCAGAAA ACAAGGTGTC AGAAGCACAG 
GCTAATTCTG GCAGCGGTGC TTCCTCCACC ACCACTGCCA CCTCAACCAC ATCTACCACC 
ACCACCACTG CCGCCTCCAC CACGCCCACA CCCCCTACTG CACCCACCCC TGTCACTTCT 
GCTCCAGCCC TGGTTGCTGC CACGGCTATT TCCACCATTG TCGTAGCTGC TTCGACCACA 
GTGACTACCC CCACGACTGC TACCACTACT GTTTCAATTT CTCCCACTAC TAAGGGCAGC 
AAATCTCCAG CGAAGGTGAG TGATGGGGGC AGCAGCAGTA CAGACTTTAA GATGGTGTCC 
TCTGGCCTCA CTGAAAACCA GCTACAGCTC TCTGTAGAGG TGTTGACATC CCACTCTTGT 
TCTGAGGAAG GCTTAGAGGA TGCAGCCAAC GTACTACTGC AGCTCTCCCG GGGGGACTCT 
GGGACCCGGG ACACTGTTCT CAAGCTGCTA CTGAATGGAG CCCGCCATCT GGGTTATACC 
CTTTGTAAAC AAATAGGTAC CCTGCTGGCC GAGCTGCGGG AATACAACCT CGAGCAGCAG 
CGGCGAGCCC AATGTGAAAC CCTCTCTCCT GATGGCCTGC CTGAGGAGCA GCCACAGACC 
ACCAAGCTGA AGGGCAAAAT GCAGAGCAGG TTTGACATGG CTGAGAATGT GGTAATTGTG 
GCATCTCAGA AGCGACCTTT GGGTGGCCGG GAGCTCCAGC TGCCTTCTAT GTCCATGTTG 
ACATCCAAGA CATCTACCCA GAAGTTCTTC TTGAGGGTAC TACAGGTCAT CATCCAGCTC 
CGGGACGACA CGCGCCGGGC TAACAAGAAA GCCAAGCAGA CAGGCAGGCT AGGTTCCTCC 
GGTTTAGGCT CAGCTAGCAG CATCCAGGCA GCTGTTCGGC AGCTGGAGGC TGAGGCTGAT 
GCCATTATAC AAATGGTACG TGAGGGTCAA AGGGCGCGGA GACAGCAACA AGCAGCAACG 
TCGGAGTCTA GCCAGTCAGA GGCGTCTGTC CGGAGGGAGG AATCACCCAT GGATGTGGAC 
CAGCCATCTC CCAGTGCTCA AGATACTCAA TCCATTGCCT CCGATGGAAC CCCACAGGGG 
GAGAAGGAAA AGGAAGAAAG ACCACCTGAG TTACCCCTGC TCAGCGAGCA GCTGAGTTTG 
GACGAGCTGT GGGACATGCT TGGGGAGTGT CTAAAGGAAC TAGAGGAATC CCATGACCAG 
CATGCGGTGC TAGTGCTACA GCCTGCTGTC GAGGCCTTCT TTCTGGTCCA TGCCACAGAG 
CGGGAGAGCA AGCCTCCTGT CCGAGACACC CGTGAGAGCC AGCTGGCACA CATCAAGGAC 
GAGCCTCCTC CACTCTCCCC TGCCCCCTTA ACCCCAGCCA CGCCTTCCTC CCTTGACCCA 
TTCTTCTCCC GGGAGCCCTC ATCTATGCAC ATCTCCTCAA GCCTGCCCCC TGACACACAG 
AAGTTCCTTC GCTTTGCAGA GACTCACCGC ACTGTGTTAA ACCAGATCCT ACGGCAGTCC 
ACGACCCACC TTGCTGATGG GCCTTTTGCT GTCCTGGTAG ACTACATTCG TGTCCTCGAC 
TTTGATGTCA AGCGCAAATA TTTCCGCCAA GAGCTGGAGC GTTTAGATGA GGGGCTCCGG 
AAAGAAGACA TGGCTGTGCA TGTCCGTCGT GACCATGTGT TTGAAGACTC CTATCGTGAG 
CTGCATCGCA AATCCCCCGA AGAAATGAAG AATCGATTGT ATATAGTATT TGAAGGAGAA 
GAAGGGCAGG ATGCTGGTGG GCTCCTGCGG GAGTGGTATA TGATCATCTC TCGAGAGATG 
TTTAACCCTA TGTATGCCTT GTTCCGTACC TCACCTGGTG ATCGAGTCAC CTACACCATC 
AATCCATCTT CCCACTGCAA CCCCAACCAC CTCAGCTACT TCAAGTTTGT CGGACGCATT 
GTGGCCAAAG CTGTATATGA CAACCGTCTT CTGGAGTGCT ACTTTACTCG ATCCTTTTAC 
AAACACATCT TGGGCAAGTC AGTCAGATAT ACAGATATGG AGAGTGAAGA TTACCACTTC 
TACCAAGGTC TGGTTTATCT GCTGGAAAAT GATGTCTCCA CACTAGGCTA TGACCTCACC 
TTCAGCACTG AGGTCCAAGA GTTTGGAGTT TGTGAAGTTC GTGACCTCAA ACCCAATGGG 
GCCAACATCT TGGTAACAGA GGAGAATAAG AAGGAGTATG TACACCTGGT ATGCCAGATG 
AGAATGACAG GAGCCATCCG CAAGCAGTTG GCGGCTTTCT TAGAAGGCTT CTATGAGATC 
ATTCCAAAGC GCCTCATTTC CATCTTCACT GAGCAGGAGT TAGAGCTGCT TATATCAGGA 
CTGCCCACCA TTGACATCGA TGATCTGAAA TCCAACACTG AATACCACAA GTACCAGTCC 
AACTCTATTC AGATCCAGTG GTTCTGGAGA GCATTGCGTT CTTTCGATCA AGCTGACCGT 
GCCAAGTTCC TCCAGTTTGT CACAGGTACT TCCAAGGTAC CCCTGCAAGG CTTTGCTGCC 
CTCGAAGGCA TGAATGGCAT TCAGAAGTTT CAGATCCATC GAGATGACAG GTCCACAGAT 
CGCCTGCCTT CAGCTCACAC ATGTTTTAAT CAGCTGGATC TGCCTGCCTA TGAGAGCTTT 
GAGAAGCTCC GCCACATGCT ACTGTTGGCT ATCCAGGAGT GCTCTGAAGG CTTTGGGCTG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884680.1
CDS	3018..16523
Translation	MDPFISRREQKCMEIGTPRKNGVLVLRSSEAVYHLTTPFQIVCLHLPLSKSKPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029191.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029191.1

ORF Insert Sequence:

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GTTGCAGATG TGGCTATGGA AAGCAGTCCA GGCTCATCCA TCTCTATGGA GCACAGGCTG 
GATGTTGAAT TAAGGGCATC AGGTTCCAGC AGCAGCACTA ACATCTCTTC TGGCCCCAGC 
CCTGGTCCCA GTCCCGGCCC CGGCACCGGC CCTGGCCCCG GCCCCGGCCC CGGCCCCGGC 
CCTGGCCCCG GCCCCGGCCC CGGTCCTGGT CCCGGCCCTG GCCCCGGCCC TGGCCCTGGC 
CCCCGTCCTG GAGTCCAGTG TATTCCACAA CGAGCAGCAC TTCTGAAATC CATGTTGAAT 
TTCCTCAAGA AGGCCATCCA AGACCCTGCT TTCTCAGATG GCATACGACA TGTGATGGAT 
GGTTCTCTGC CTACCTCCCT GAAACACATC ATCAGCAATG CAGAATACTA TGGCCCATCA 
CTCTTCCTCC TAGCTACTGA AGTGGTGACT GTGTTTGTAT TTCAAGAACC ATCACTGCTC 
TCCTCACTCC AGGACAATGG ATTGACAGAT GTCATGCTGC ATGCACTGCT TATCAAAGAT 
GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC CTCCCAAATG TATTCAGTGC ACTCTGTTTG 
AATGCCCGAG GTCTTCAGTC TTTTGTTCAG TGTCAGCCTT TTGAACGCCT CTTCAAAGTT 
CTTCTGTCTC CAGATTACCT CCCAGCCATG CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG 
GATACTGCAT CCAACCTGGG GAGTGCTGTC GATGAGCTCA TGAGACATCA GCCCACCCTT 
AAAACAGATG CAACGACTGC CATCATCAAG TTACTTGAAG AAATCTGTAA TCTTGGAAGG 
GACCCCAAAT ACATCTGTCA GAAGCCATCA ATCCAGAAGG CAGATGGCAC TGCCACTGCT 
CCTCCCCCAA GGTCTAATCA TGCCGCAGAA GAAGCCTCTA GTGAGGATGA GGAGGAAGAG 
GAAGTACAGG CCATGCAGAG CTTTAATTCT ACCCAGCAAA ATGAAACTGA GCCTAATCAG 
CAGGTTGTTG GTACAGAGGA ACGTATTCCT ATTCCCCTCA TGGATTACAT CCTTAATGTG 
ATGAAATTTG TGGAATCTAT TCTGAGCAAC AATACAACAG ATGACCACTG CCAGGAATTT 
GTGAATCAGA AAGGACTGTT GCCTTTGGTT ACCATTTTGG GTCTTCCCAA TCTGCCCATT 
GACTTTCCCA CATCTGCTGC CTGTCAGGCT GTTGCAGGTG TCTGCAAATC CATATTGACA 
CTGTCACATG AACCCAAAGT CCTTCAAGAG GGTCTCCTTC AGTTGGACTC CATCCTCTCC 
TCCCTGGAGC CCTTACACCG CCCCATTGAA TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA 
CTGGCTTGCG CAGGCAATGT TGCTGATGCT ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG 
CATGCACTCA CTGCTGCCCA TGCCTACATC ATGATGTTTG TTCATACTTG CAGAGTTGGA 
CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC CAGTGGGGCT CTCAATTGGG TCTGAGTGTT 
TTGAGCAAGC TGAGCCAGTT ATACTGTTCC CTGGTGTGGG AAAGCACTGT CCTCCTCTCT 
CTGTGTACCC CAAACAGCCT ACCATCTGGG TGTGAATTTG GCCAGGCAGA TATGCAGAAA 
CTGGTTCCAA AGGATGAGAA GGCAGGTACG ACCCAGGGCG GAAAAAGATC AGATGGGGAA 
CAGGATGGAG CAGCTGGAAG TATGGATGCT TCTACCCAGG GCTTATTAGA AGGCATTGGG 
CTAGATGGTG ACACATTGGC TCCCATGGAG ACAGATGAAC CTACTGCTTC AGACTCTAAG 
GGCAAATCTA AAATCACACC AGCAATGGCT GCCAGAATTA AGCAAATCAA GCCTTTGTTA 
TCAGCTTCCT CCAGATTAGG CCGAGCACTT GCTGAGCTAT TTGGACTTCT TGTTAAACTT 
TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG AGCCATCATG CTGCCAGCAC CACTACAGCA 
CCGACACCTG CCGCGCGATC AACAGCCTCA GCTCTCACTA AGCTCTTGAC TAAGGGGTTA 
TCTTGGCAGC CCCCACCATA TACACCTACT CCCCGATTCA GGCTGACATT CTTCATCTGT 
TCAGTTGGTT TCACATCCCC AATGCTGTTT GATGAGAGGA AGTATCCCTA CCACCTCATG 
CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC AATGCTCTTT TTGAAACTTT CAACTGGGCT 
CTGTCCATGG GAGGTAAAGT TCCTGTTTCT GAGGGATTGG AACACTCAGA CTTGCCTGAT 
GGCACAGGAG AATTCCTAGA TGCCTGGCTT ATGCTGGTGG AGAAGATGGT GAATCCCACC 
ACGGTGCTTG AATCTCCACA TTCGCTGCCT GCCAAATTGC CTGGAGGTGT CCAGAACTTT 
CCCCAGTTCA GTGCACTGCG CTTCCTTGTG GTAACTCAGA AAGCAGCCTT TACTTGCATC 
AAAAACTTAT GGAACCGGAA ACCCCTGAAG GTATATGGTG GACGAATGGC TGAATCGATG 
CTGGCCATTC TATGCCACAT CCTCCGAGGA GAACCTGTGA TTCGAGAGAG ACTAAGCAAG 
GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG 
GAACCTCAAG TCAACCAGCA ACAACTGCAA CAGCTCATGG ACATGGGCTT CACAAGGGAA 
CATGCAATGG AGGCACTGTT GAACACCAGC ACCATGGAGC AGGCCACAGA GTACCTTTTA 
ACCCACCCTC CTCCAATCAT GGGAGGAGTT GTTCGGGATC TCAGCATGTC TGAAGAGGAC 
CAGATGATGA GAGCAATTGC TATGTCTCTG GGACAGGATA TTCCAATGGA TCAAAGGGCA 
GAGTCACCTG AGGAAGTTGC TTGCCGGAAG GAGGAAGAGG AACGGAAAGC TCGGGAAAAG 
CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG AAGTTCCAGG ATGCTGACCC GTTGGAACAA 
GATGAGCTCC ACACTTTCAC AGATACTATG TTGCCAGGCT GCTTCCACCT TCTTGATGAG 
CTGCCAGACA CAGTATACCG TGTGTGTGAC CTGATCATGA CAGCAATCAA ACGTAATGGA 
GCAGATTATC GTGACATGAT TCTGAAGCAA GTAGTCAATC AGGTGTGGGA AGCTGCTGAT 
GTATTGATCA AAGCTGCTCT TCCCCTGACA ACAAGTGACA CAAAAACCGT GTCAGAGTGG 
ATAAGTCAGA TGGCCACACT GCCCCAGGCC TCCAATTTGG CTACTAGAAT CTTGCTTTTA 
ACGCTACTTT TTGAGGAGTT GAAGCTACCT TGTGCTTGGG TGGTTGAATC AAGTGGCATC 
CTTAATGTCC TAATCAAACT CTTGGAAGTG GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG 
CAGAAGGAAG TCCAGACCCC AAAGTGGATC ACACCAGTGT TGCTCCTGAT TGATTTCTAT 
GAAAAGACAG CCATCTCCTC AAAAAGGAGA GCCCAGATGA CTAAGTACCT GCAATCCAAC 
AGCAACAACT GGCGCTGGTT TGATGATCGC TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC 
AACAATAGCA CTATTGATTC TGCCTGGAAA TCTGGAGAGA CAAGCGTGCG ATTCACTGCA 
GGCCGAAGAA GATACACGGT CCAATTCACT ACAATGGTGC AGGTTAATGA GGAAACAGGG 
AACCGACGCC CTGTGATGCT GACTCTCCTC AGGGTACCTC GGCTGAATAA AAATTCAAAA 
AACAGCAATG GACAGGAACT AGAGAAGACG CTGGAAGAAA GCAAAGAAAT GGATATCAAA 
CGTAAAGAAA ATAAAGGCAA TGATACCCCT TTGGCCCTAG AGAGTACAAA CACTGAAAAG 
GAGACAAGCC TGGAGGAAAC AAAAATCGGG GAGATCCTGA TCCAGGGCTT GACAGAAGAT 
ATGGTGACTG TTTTAATCCG GGCCTGCGTG AGCATGCTGG GAGTCCCTGT GGACCCAGAT 
ACTTTGCATG CCACCCTTCG TCTCTGTCTG AGGCTCACCC GGGACCACAA ATATGCCATG 
ATGTTTGCAG AACTGAAGAG TACCCGCATG ATCTTGAATT TGACCCAGAG CTCAGGCTTC 
AATGGGTTTA CTCCCCTGGT CACCCTTCTC TTAAGACACA TCATTGAGGA CCCCTGTACC 
CTTCGTCATA CCATGGAAAA GGTTGTTCGC TCAGCAGCTA CAAGTGGAGC TGGTAGCACT 
ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC TCTCGGGAGA TCAACTACAT CCTTCGTGTC 
CTTGGGCCAG CCGCATGCCG CAATCCAGAC ATATTCACAG AAGTGGCCAA CTGCTGTATC 
CGCATCGCCC TTCCTGCCCC TCGAGGCTCA GGAACTGCTT CAGATGATGA ATTTGAGAAT 
CTTAGAATTA AAGGCCCTAA TGCTGTACAG CTGGTGAAGA CCACCCCTTT GAAGCCCTCA 
CCTCTGCCTG TCATCCCTGA TACTATCAAG GAAGTGATCT ATGATATGCT GAATGCTCTG 
GCTGCATACC ATGCTCCAGA GGAAGCAGAT AAATCTGATC CTAAACCTGG GGTTATGACC 
CAAGAGGTTG GCCAGCTCCT GCAAGACATG GGTGATGATG TATACCAGCA GTACCGGTCA 
CTTACGCGTC AGAGCAGTGA CTTTGATACG CAGTCAGGTT TTTCCATTAA TAGTCAGGTC 
TTTGCTGCAG ATGGTGCCTC CACTGAGACT TCCGCATCTG GGACCTCCCA AGGAGAGGCT 
TCAACTCCAG AGGAGTCTCG AGATGGGAAG AAAGATAAAG AAGGGGACCG GGCCTCTGAG 
GAAGGCAAAC AGAAAGGCAA GGGCAGCAAA CCTTTAATGC CTACCTCCAC TATCCTTCGT 
CTTCTGGCAG AGTTGGTGAG GTCCTATGTT GGTATTGCTA CCCTGATTGC CAACTACAGC 
TACACTGTGG GCCAGTCTGA ACTGATCAAA GAGGACTGCA GTGTGCTAGC TTTTGTTCTG 
GACCACCTGC TCCCACATAC CCAGAATGCA GAAGACAAGG ACACCCCTGC CTTGGCCCGC 
CTGTTCCTCG CAAGCCTGGC TGCTGCAGGG AGTGGCACAG ATGCCCAGGT GGCCCTAGTG 
AATGAAGTAA AAGCAGCCCT TGGACGGGCA CTGGCTATGG CTGAGAGTAC AGAGAAACAT 
GCCAGGCTTC AGGCAGTGAT GTGTATCATC AGTACTATCA TGGAGTCCTG CCCCTCCACC 
TCCAGCTTCT ACAGCAGTGC CACAGCGAAG ACCCAGCACA ATGGCATGAA CAACATCATT 
CGGCTTTTCC TGAAGAAGGG ACTGGTTAAT GACCTGGCCA GAGTACCTCA CAGCTTAGAC 
CTGTCCAGTC CCAACATGGC CAACACAGTC AATGCTGCTC TGAAGCCTTT GGAAACACTT 
TCCCGGATTG TGAACCAGCC CAGTAGCCTT TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC 
AAGTCTGAGC AGGATGCCCA AGGAGCCTCT CAAGATTCCA GTAGCAACCA GCAGGACCCA 
GGCGAGCCTG GGGAAGCAGA AGTGCAGGAG GAGGATCATG ATGTCACTCA GACAGAGGTG 
GCAGATGGGG ATATCATGGA TGGGGAGGCT GAAACCGACT CAGTGGTGAT TGCTGGGCAG 
CCTGAGGTGC TCAGTTCACA AGAGATGCAG GTTGAGAATG AGCTGGAGGA CCTGATAGAT 
GAGTTGCTTG AGAGGGATGG CGGATCTGGG AACAGTACAA TTATAGTGAG CAGAAGTGGA 
GAGGATGAAT CACAAGAGGA CGTGCTGATG GATGAAGCTC CTTCCAACCT CAGCCAAGCT 
TCCACCTTGC AGGCCAACCG AGAAGATTCC ATGAATATCC TGGACCCTGA GGATGAGGAG 
GAGCACACTC AGGAAGAGGA CAGCAGTGGC AGTAACGAGG ATGAGGATGA TAGTCAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGAGGAA GATGATCAGG AGGATGATGA AGGTGAAGAG 
GGAGATGAAG ACGATGACGA CGATGGCTCT GAGATGGAAT TGGATGAGGA TTATCCTGAT 
ATGAACGCTT CTCCCTTGGT CCGATTTGAG CGCTTTGACC GGGAGGATGA TCTCATCATT 
GAGTTTGACA ACATGTTCTC CAGTGCTACA GACATCCCCC CATCCCCAGG AAATATCCCT 
ACCACCCATC CACTGATGGT GCGCCATGCA GACCACAGTT CTCTGACACT GGGCAGTGGC 
TCTTCAACAA CTCGTCTCAC CCAGGGCATC GGGCGCAGTC AGAGGACCCT AAGGCAGCTG 
ACGGCCAATA CTGGCCACAC CATTCATGTT CACTACCCTG GGAATCGCCA GCCCAACCCT 
CCTCTTATAC TGCAGAGGTT GCTTGGTCCC TCAGCTGCTG CTGACATCCT TCAGCTGAGC 
AGCAGCCTTC CCCTACAAAG CCGGGGTCGG GCCCGCCTCC TGGTAGGCAA CGATGACGTC 
CACATCATCG CCCGTTCTGA TGATGAGCTG CTGGATGACT TTTTCCATGA TCAGAGCACA 
GCTACCAGCC AAGCAGGAAC CCTGTCCAGC ATCCCCACAG CCCTGACCCG CTGGACAGAA 
GAATGCAAAG TTCTCGATGC TGAGAGCATG CATGACTGTG TTTCAGTGGT TAAAGTGTCC 
ATTGTCAATC ACCTGGAATT CCTGAGGGAT GAGGAGCTGG AAGAAAGGCG AGAGAAGCGC 
AGGAAACAAC TGGCTGAGGA AGAAACAAAG ATAACTGATA AAGGCAAAGA AGATAAGGAG 
AACAGGGATC AGAGTGCACA GTGTACTGCA TCTAAGTCAA ATGACTCCAC TGAACAGAAT 
CTCTCAGATG GGACGCCTAT GCCTGACAGC TACCCAACAA CCCCATCTTC AACTGATGCA 
GCTACATCTG AGTCCAAGGA GACCCTTGGC ACTCTGCAAT CCTCACAACA GCAACCAACA 
CTCCCAACCC CACCAGCTTT GGGAGAGGTT CCTCAGGAGC TGCAGTCTCC AGCTGGAGAA 
GGGGGCAGCT CTACACAGCT ATTGATGCCT GTAGAGCCAG AGGAATTGGG TCCCACAAGG 
CCAAGTGGGG AAGCAGAAAC AACTCAGATG GAGTTATCCC CAGCTCCCAC TATAACCTCA 
CTTTCCCCAG AGAGAGCTGA GGATTCTGAT GCACTGACGG CTGTCAGCAG TCAGCTAGAA 
GGCTCTCCTA TGGATACAAG CAGCCTGGCT TCCTGTACCT TAGAGGAGGC TGTGGGTGAC 
ACTTCAGCAG CTGGCAGTTC TGAGCAGCCC AGAGCAGGCA GCTCCACTCC TGGGGATGCC 
CCACCAGCTG TGGCGGAAGT GCAAGGCAGG AGTGATGGGT CAGGGGAATC TGCCCAGCCA 
CCTGAGGACA GCTCCCCACC TGCATCCTCT GAGAGCTCTT CCACCAGAGA TTCTGCCGTG 
GCCATTTCTG GAGCAGATTC CCGAGGAATC CTAGAAGAGC CGTTGCCTTC AACAAGCAGT 
GAAGAAGAAG ATCCCCTTGC GGGTATCAGT CTCCCTGAAG GTGTGGACCC CTCTTTTCTG 
GCTGCCCTGC CTGATGACAT CCGTCGGGAA GTTCTACAGA ACCAGCTAGG CATTCGTCCA 
CCAACCCGGA CTGCCCCCTC CACAAATAGC TCAGCGCCTG CAGTGGTGGG GAATCCTGGT 
GTGACTGAAG TGAGCCCTGA GTTTCTGGCT GCCCTGCCTC CAGCCATTCA GGAGGAAGTA 
CTGGCACAGC AGAGAGCTGA GCAGCAGCGA CGAGAACTAG CACAGAATGC CAGCTCAGAC 
ACCCCTATGG ACCCTGTGAC CTTCATCCAG ACTCTGCCCT CAGACCTGCG CCGTAGTGTC 
CTAGAGGATA TGGAGGACAG TGTGTTAGCT GTGATGCCAC CTGACATTGC AGCTGAGGCT 
CAAGCCCTGA GACGAGAGCA AGAAGCCCGG CAGCGACAGC TCATGCATGA GCGTCTGTTT 
GGGCACAGTA GCACCTCCGC ACTCTCTGCT ATTCTCCGAA GCCCGGCTTT CACCAGTCGC 
TTAAGTGGCA ACCGTGGGGT CCAGTATACT CGCCTTGCTG TGCAGAGAGG TGGCACCTTC 
CAGATGGGGG GTAGCAGCAG CCATAACAGG CCTTCTGGCA GTAATGTAGA TACTCTCCTC 
CGCCTCCGAG GACGGCTCCT TCTGGACCAC GAAGCCCTTT CTTGTCTCTT GGTCCTACTT 
TTTGTGGATG AGCCAAAGCT CAATACTAGC CGTCTACACC GAGTACTGAG AAATCTCTGC 
TACCATGCCC AGACCCGCCA CTGGGTCATC CGCAGTCTGC TCTCCATCTT GCAGCGCAGC 
AGTGAGAGTG AGCTATGCAT TGAAACACCC AAACTCACTA CAAGTGAGGA AAAGGGCAAA 
AAGTCGAGCA AGAGCTGTGG GTCAAGTAGC CATGAGAACC GTCCCCTGGA CCTGCTACAC 
AAGATGGAGT CAAAGAGCTC CAACCAGCTT TCCTGGCTCT CAGTATCCAT GGATGCAGCC 
CTAGGCTGCA GGACTAATAT ATTTCAGATC CAGCGTTCAG GGGGGCGTAA ACATACCGAG 
AAGCATGCAA GCGGTGGCTC CACCGTCCAC ATCCATCCCC AAGCTGCTCC TGTTGTCTGC 
AGACACGTTT TGGATACACT CATTCAATTG GCCAAGGTAT TTCCCAGCCA CTTCACACAG 
CAGCGGACCA AAGAAACAAA CTGTGAGAGT GATCGGGAAA GGGGCAATAA GGCCTGTAGC 
CCATGCTCCT CACAGTCCTC CAGCAGTGGC ATTTGCACAG ACTTCTGGGA CTTATTGGTA 
AAACTGGACA ACATGAATGT CAGCCGGAAA GGCAAGAACT CCGTGAAGTC AGTGCCAGTG 
AGCGCTGGCG GTGAGGGGGA AACCTCTCCA TACAGCCTCG AGGCCTCTCC ACTGGGGCAG 
CTCATGAACA TGTTGTCACA CCCAGTCATC CGCCGGAGCT CTCTCTTAAC TGAGAAACTC 
CTCAGACTCC TTTCTCTCAT CTCAATTGCT CTCCCAGAAA ACAAGGTGTC AGAAGCACAG 
GCTAATTCTG GCAGCGGTGC TTCCTCCACC ACCACTGCCA CCTCAACCAC ATCTACCACC 
ACCACCACTG CCGCCTCCAC CACGCCCACA CCCCCTACTG CACCCACCCC TGTCACTTCT 
GCTCCAGCCC TGGTTGCTGC CACGGCTATT TCCACCATTG TCGTAGCTGC TTCGACCACA 
GTGACTACCC CCACGACTGC TACCACTACT GTTTCAATTT CTCCCACTAC TAAGGGCAGC 
AAATCTCCAG CGAAGGTGAG TGATGGGGGC AGCAGCAGTA CAGACTTTAA GATGGTGTCC 
TCTGGCCTCA CTGAAAACCA GCTACAGCTC TCTGTAGAGG TGTTGACATC CCACTCTTGT 
TCTGAGGAAG GCTTAGAGGA TGCAGCCAAC GTACTACTGC AGCTCTCCCG GGGGGACTCT 
GGGACCCGGG ACACTGTTCT CAAGCTGCTA CTGAATGGAG CCCGCCATCT GGGTTATACC 
CTTTGTAAAC AAATAGGTAC CCTGCTGGCC GAGCTGCGGG AATACAACCT CGAGCAGCAG 
CGGCGAGCCC AATGTGAAAC CCTCTCTCCT GATGGCCTGC CTGAGGAGCA GCCACAGACC 
ACCAAGCTGA AGGGCAAAAT GCAGAGCAGG TTTGACATGG CTGAGAATGT GGTAATTGTG 
GCATCTCAGA AGCGACCTTT GGGTGGCCGG GAGCTCCAGC TGCCTTCTAT GTCCATGTTG 
ACATCCAAGA CATCTACCCA GAAGTTCTTC TTGAGGGTAC TACAGGTCAT CATCCAGCTC 
CGGGACGACA CGCGCCGGGC TAACAAGAAA GCCAAGCAGA CAGGCAGGCT AGGTTCCTCC 
GGTTTAGGCT CAGCTAGCAG CATCCAGGCA GCTGTTCGGC AGCTGGAGGC TGAGGCTGAT 
GCCATTATAC AAATGGTACG TGAGGGTCAA AGGGCGCGGA GACAGCAACA AGCAGCAACG 
TCGGAGTCTA GCCAGTCAGA GGCGTCTGTC CGGAGGGAGG AATCACCCAT GGATGTGGAC 
CAGCCATCTC CCAGTGCTCA AGATACTCAA TCCATTGCCT CCGATGGAAC CCCACAGGGG 
GAGAAGGAAA AGGAAGAAAG ACCACCTGAG TTACCCCTGC TCAGCGAGCA GCTGAGTTTG 
GACGAGCTGT GGGACATGCT TGGGGAGTGT CTAAAGGAAC TAGAGGAATC CCATGACCAG 
CATGCGGTGC TAGTGCTACA GCCTGCTGTC GAGGCCTTCT TTCTGGTCCA TGCCACAGAG 
CGGGAGAGCA AGCCTCCTGT CCGAGACACC CGTGAGAGCC AGCTGGCACA CATCAAGGAC 
GAGCCTCCTC CACTCTCCCC TGCCCCCTTA ACCCCAGCCA CGCCTTCCTC CCTTGACCCA 
TTCTTCTCCC GGGAGCCCTC ATCTATGCAC ATCTCCTCAA GCCTGCCCCC TGACACACAG 
AAGTTCCTTC GCTTTGCAGA GACTCACCGC ACTGTGTTAA ACCAGATCCT ACGGCAGTCC 
ACGACCCACC TTGCTGATGG GCCTTTTGCT GTCCTGGTAG ACTACATTCG TGTCCTCGAC 
TTTGATGTCA AGCGCAAATA TTTCCGCCAA GAGCTGGAGC GTTTAGATGA GGGGCTCCGG 
AAAGAAGACA TGGCTGTGCA TGTCCGTCGT GACCATGTGT TTGAAGACTC CTATCGTGAG 
CTGCATCGCA AATCCCCCGA AGAAATGAAG AATCGATTGT ATATAGTATT TGAAGGAGAA 
GAAGGGCAGG ATGCTGGTGG GCTCCTGCGG GAGTGGTATA TGATCATCTC TCGAGAGATG 
TTTAACCCTA TGTATGCCTT GTTCCGTACC TCACCTGGTG ATCGAGTCAC CTACACCATC 
AATCCATCTT CCCACTGCAA CCCCAACCAC CTCAGCTACT TCAAGTTTGT CGGACGCATT 
GTGGCCAAAG CTGTATATGA CAACCGTCTT CTGGAGTGCT ACTTTACTCG ATCCTTTTAC 
AAACACATCT TGGGCAAGTC AGTCAGATAT ACAGATATGG AGAGTGAAGA TTACCACTTC 
TACCAAGGTC TGGTTTATCT GCTGGAAAAT GATGTCTCCA CACTAGGCTA TGACCTCACC 
TTCAGCACTG AGGTCCAAGA GTTTGGAGTT TGTGAAGTTC GTGACCTCAA ACCCAATGGG 
GCCAACATCT TGGTAACAGA GGAGAATAAG AAGGAGTATG TACACCTGGT ATGCCAGATG 
AGAATGACAG GAGCCATCCG CAAGCAGTTG GCGGCTTTCT TAGAAGGCTT CTATGAGATC 
ATTCCAAAGC GCCTCATTTC CATCTTCACT GAGCAGGAGT TAGAGCTGCT TATATCAGGA 
CTGCCCACCA TTGACATCGA TGATCTGAAA TCCAACACTG AATACCACAA GTACCAGTCC 
AACTCTATTC AGATCCAGTG GTTCTGGAGA GCATTGCGTT CTTTCGATCA AGCTGACCGT 
GCCAAGTTCC TCCAGTTTGT CACAGGTACT TCCAAGGTAC CCCTGCAAGG CTTTGCTGCC 
CTCGAAGGCA TGAATGGCAT TCAGAAGTTT CAGATCCATC GAGATGACAG GTCCACAGAT 
CGCCTGCCTT CAGCTCACAC ATGTTTTAAT CAGCTGGATC TGCCTGCCTA TGAGAGCTTT 
GAGAAGCTCC GCCACATGCT ACTGTTGGCT ATCCAGGAGT GCTCTGAAGG CTTTGGGCTG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029192.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884681.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X2, mRNA.

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CloneID	OHu99113
Clone ID Related Accession (Same CDS sequence)	XM_017029192.1
Accession Version	XM_017029192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GTTGCAGATG TGGCTATGGA AAGCAGTCCA GGCTCATCCA TCTCTATGGA GCACAGGCTG 
GATGTTGAAT TAAGGGCATC AGGTTCCAGC AGCAGCACTA ACATCTCTTC TGGCCCCAGC 
CCTGGTCCCA GTCCCGGCCC CGGCACCGGC CCTGGCCCCG GCCCCGGCCC CGGCCCCGGC 
CCTGGCCCCG GCCCCGGCCC CGGTCCTGGT CCCGGCCCTG GCCCCGGCCC TGGCCCTGGC 
CCCCGTCCTG GAGTCCAGTG TATTCCACAA CGAGCAGCAC TTCTGAAATC CATGTTGAAT 
TTCCTCAAGA AGGCCATCCA AGACCCTGCT TTCTCAGATG GCATACGACA TGTGATGGAT 
GGTTCTCTGC CTACCTCCCT GAAACACATC ATCAGCAATG CAGAATACTA TGGCCCATCA 
CTCTTCCTCC TAGCTACTGA AGTGGTGACT GTGTTTGTAT TTCAAGAACC ATCACTGCTC 
TCCTCACTCC AGGACAATGG ATTGACAGAT GTCATGCTGC ATGCACTGCT TATCAAAGAT 
GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC CTCCCAAATG TATTCAGTGC ACTCTGTTTG 
AATGCCCGAG GTCTTCAGTC TTTTGTTCAG TGTCAGCCTT TTGAACGCCT CTTCAAAGTT 
CTTCTGTCTC CAGATTACCT CCCAGCCATG CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG 
GATACTGCAT CCAACCTGGG GAGTGCTGTC GATGAGCTCA TGAGACATCA GCCCACCCTT 
AAAACAGATG CAACGACTGC CATCATCAAG TTACTTGAAG AAATCTGTAA TCTTGGAAGG 
GACCCCAAAT ACATCTGTCA GAAGCCATCA ATCCAGAAGG CAGATGGCAC TGCCACTGCT 
CCTCCCCCAA GGTCTAATCA TGCCGCAGAA GAAGCCTCTA GTGAGGATGA GGAGGAAGAG 
GAAGTACAGG CCATGCAGAG CTTTAATTCT ACCCAGCAAA ATGAAACTGA GCCTAATCAG 
CAGGTTGTTG GTACAGAGGA ACGTATTCCT ATTCCCCTCA TGGATTACAT CCTTAATGTG 
ATGAAATTTG TGGAATCTAT TCTGAGCAAC AATACAACAG ATGACCACTG CCAGGAATTT 
GTGAATCAGA AAGGACTGTT GCCTTTGGTT ACCATTTTGG GTCTTCCCAA TCTGCCCATT 
GACTTTCCCA CATCTGCTGC CTGTCAGGCT GTTGCAGGTG TCTGCAAATC CATATTGACA 
CTGTCACATG AACCCAAAGT CCTTCAAGAG GGTCTCCTTC AGTTGGACTC CATCCTCTCC 
TCCCTGGAGC CCTTACACCG CCCCATTGAA TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA 
CTGGCTTGCG CAGGCAATGT TGCTGATGCT ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG 
CATGCACTCA CTGCTGCCCA TGCCTACATC ATGATGTTTG TTCATACTTG CAGAGTTGGA 
CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC CAGTGGGGCT CTCAATTGGG TCTGAGTGTT 
TTGAGCAAGC TGAGCCAGTT ATACTGTTCC CTGGTGTGGG AAAGCACTGT CCTCCTCTCT 
CTGTGTACCC CAAACAGCCT ACCATCTGGG TGTGAATTTG GCCAGGCAGA TATGCAGAAA 
CTGGTTCCAA AGGATGAGAA GGCAGGTACG ACCCAGGGCG GAAAAAGATC AGATGGGGAA 
CAGGATGGAG CAGCTGGAAG TATGGATGCT TCTACCCAGG GCTTATTAGA AGGCATTGGG 
CTAGATGGTG ACACATTGGC TCCCATGGAG ACAGATGAAC CTACTGCTTC AGACTCTAAG 
GGCAAATCTA AAATCACACC AGCAATGGCT GCCAGAATTA AGCAAATCAA GCCTTTGTTA 
TCAGCTTCCT CCAGATTAGG CCGAGCACTT GCTGAGCTAT TTGGACTTCT TGTTAAACTT 
TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG AGCCATCATG CTGCCAGCAC CACTACAGCA 
CCGACACCTG CCGCGCGATC AACAGCCTCA GCTCTCACTA AGCTCTTGAC TAAGGGGTTA 
TCTTGGCAGC CCCCACCATA TACACCTACT CCCCGATTCA GGCTGACATT CTTCATCTGT 
TCAGTTGGTT TCACATCCCC AATGCTGTTT GATGAGAGGA AGTATCCCTA CCACCTCATG 
CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC AATGCTCTTT TTGAAACTTT CAACTGGGCT 
CTGTCCATGG GAGGTAAAGT TCCTGTTTCT GAGGGATTGG AACACTCAGA CTTGCCTGAT 
GGCACAGGAG AATTCCTAGA TGCCTGGCTT ATGCTGGTGG AGAAGATGGT GAATCCCACC 
ACGGTGCTTG AATCTCCACA TTCGCTGCCT GCCAAATTGC CTGGAGGTGT CCAGAACTTT 
CCCCAGTTCA GTGCACTGCG CTTCCTTGTG GTAACTCAGA AAGCAGCCTT TACTTGCATC 
AAAAACTTAT GGAACCGGAA ACCCCTGAAG GTATATGGTG GACGAATGGC TGAATCGATG 
CTGGCCATTC TATGCCACAT CCTCCGAGGA GAACCTGTGA TTCGAGAGAG ACTAAGCAAG 
GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG 
GAACCTCAAG TCAACCAGCA ACAACTGCAA CAGCTCATGG ACATGGGCTT CACAAGGGAA 
CATGCAATGG AGGCACTGTT GAACACCAGC ACCATGGAGC AGGCCACAGA GTACCTTTTA 
ACCCACCCTC CTCCAATCAT GGGAGGAGTT GTTCGGGATC TCAGCATGTC TGAAGAGGAC 
CAGATGATGA GAGCAATTGC TATGTCTCTG GGACAGGATA TTCCAATGGA TCAAAGGGCA 
GAGTCACCTG AGGAAGTTGC TTGCCGGAAG GAGGAAGAGG AACGGAAAGC TCGGGAAAAG 
CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG AAGTTCCAGG ATGCTGACCC GTTGGAACAA 
GATGAGCTCC ACACTTTCAC AGATACTATG TTGCCAGGCT GCTTCCACCT TCTTGATGAG 
CTGCCAGACA CAGTATACCG TGTGTGTGAC CTGATCATGA CAGCAATCAA ACGTAATGGA 
GCAGATTATC GTGACATGAT TCTGAAGCAA GTAGTCAATC AGGTGTGGGA AGCTGCTGAT 
GTATTGATCA AAGCTGCTCT TCCCCTGACA ACAAGTGACA CAAAAACCGT GTCAGAGTGG 
ATAAGTCAGA TGGCCACACT GCCCCAGGCC TCCAATTTGG CTACTAGAAT CTTGCTTTTA 
ACGCTACTTT TTGAGGAGTT GAAGCTACCT TGTGCTTGGG TGGTTGAATC AAGTGGCATC 
CTTAATGTCC TAATCAAACT CTTGGAAGTG GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG 
CAGAAGGAAG TCCAGACCCC AAAGTGGATC ACACCAGTGT TGCTCCTGAT TGATTTCTAT 
GAAAAGACAG CCATCTCCTC AAAAAGGAGA GCCCAGATGA CTAAGTACCT GCAATCCAAC 
AGCAACAACT GGCGCTGGTT TGATGATCGC TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC 
AACAATAGCA CTATTGATTC TGCCTGGAAA TCTGGAGAGA CAAGCGTGCG ATTCACTGCA 
GGCCGAAGAA GATACACGGT CCAATTCACT ACAATGGTGC AGGTTAATGA GGAAACAGGG 
AACCGACGCC CTGTGATGCT GACTCTCCTC AGGGTACCTC GGCTGAATAA AAATTCAAAA 
AACAGCAATG GACAGGAACT AGAGAAGACG CTGGAAGAAA GCAAAGAAAT GGATATCAAA 
CGTAAAGAAA ATAAAGGCAA TGATACCCCT TTGGCCCTAG AGAGTACAAA CACTGAAAAG 
GAGACAAGCC TGGAGGAAAC AAAAATCGGG GAGATCCTGA TCCAGGGCTT GACAGAAGAT 
ATGGTGACTG TTTTAATCCG GGCCTGCGTG AGCATGCTGG GAGTCCCTGT GGACCCAGAT 
ACTTTGCATG CCACCCTTCG TCTCTGTCTG AGGCTCACCC GGGACCACAA ATATGCCATG 
ATGTTTGCAG AACTGAAGAG TACCCGCATG ATCTTGAATT TGACCCAGAG CTCAGGCTTC 
AATGGGTTTA CTCCCCTGGT CACCCTTCTC TTAAGACACA TCATTGAGGA CCCCTGTACC 
CTTCGTCATA CCATGGAAAA GGTTGTTCGC TCAGCAGCTA CAAGTGGAGC TGGTAGCACT 
ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC TCTCGGGAGA TCAACTACAT CCTTCGTGTC 
CTTGGGCCAG CCGCATGCCG CAATCCAGAC ATATTCACAG AAGTGGCCAA CTGCTGTATC 
CGCATCGCCC TTCCTGCCCC TCGAGGCTCA GGAACTGCTT CAGATGATGA ATTTGAGAAT 
CTTAGAATTA AAGGCCCTAA TGCTGTACAG CTGGTGAAGA CCACCCCTTT GAAGCCCTCA 
CCTCTGCCTG TCATCCCTGA TACTATCAAG GAAGTGATCT ATGATATGCT GAATGCTCTG 
GCTGCATACC ATGCTCCAGA GGAAGATAAA TCTGATCCTA AACCTGGGGT TATGACCCAA 
GAGGTTGGCC AGCTCCTGCA AGACATGGGT GATGATGTAT ACCAGCAGTA CCGGTCACTT 
ACGCGTCAGA GCAGTGACTT TGATACGCAG TCAGGTTTTT CCATTAATAG TCAGGTCTTT 
GCTGCAGATG GTGCCTCCAC TGAGACTTCC GCATCTGGGA CCTCCCAAGG AGAGGCTTCA 
ACTCCAGAGG AGTCTCGAGA TGGGAAGAAA GATAAAGAAG GGGACCGGGC CTCTGAGGAA 
GGCAAACAGA AAGGCAAGGG CAGCAAACCT TTAATGCCTA CCTCCACTAT CCTTCGTCTT 
CTGGCAGAGT TGGTGAGGTC CTATGTTGGT ATTGCTACCC TGATTGCCAA CTACAGCTAC 
ACTGTGGGCC AGTCTGAACT GATCAAAGAG GACTGCAGTG TGCTAGCTTT TGTTCTGGAC 
CACCTGCTCC CACATACCCA GAATGCAGAA GACAAGGACA CCCCTGCCTT GGCCCGCCTG 
TTCCTCGCAA GCCTGGCTGC TGCAGGGAGT GGCACAGATG CCCAGGTGGC CCTAGTGAAT 
GAAGTAAAAG CAGCCCTTGG ACGGGCACTG GCTATGGCTG AGAGTACAGA GAAACATGCC 
AGGCTTCAGG CAGTGATGTG TATCATCAGT ACTATCATGG AGTCCTGCCC CTCCACCTCC 
AGCTTCTACA GCAGTGCCAC AGCGAAGACC CAGCACAATG GCATGAACAA CATCATTCGG 
CTTTTCCTGA AGAAGGGACT GGTTAATGAC CTGGCCAGAG TACCTCACAG CTTAGACCTG 
TCCAGTCCCA ACATGGCCAA CACAGTCAAT GCTGCTCTGA AGCCTTTGGA AACACTTTCC 
CGGATTGTGA ACCAGCCCAG TAGCCTTTTT GGCAGCAAGA GTGCTTCTAG CAAGAACAAG 
TCTGAGCAGG ATGCCCAAGG AGCCTCTCAA GATTCCAGTA GCAACCAGCA GGACCCAGGC 
GAGCCTGGGG AAGCAGAAGT GCAGGAGGAG GATCATGATG TCACTCAGAC AGAGGTGGCA 
GATGGGGATA TCATGGATGG GGAGGCTGAA ACCGACTCAG TGGTGATTGC TGGGCAGCCT 
GAGGTGCTCA GTTCACAAGA GATGCAGGTT GAGAATGAGC TGGAGGACCT GATAGATGAG 
TTGCTTGAGA GGGATGGCGG ATCTGGGAAC AGTACAATTA TAGTGAGCAG AAGTGGAGAG 
GATGAATCAC AAGAGGACGT GCTGATGGAT GAAGCTCCTT CCAACCTCAG CCAAGCTTCC 
ACCTTGCAGG CCAACCGAGA AGATTCCATG AATATCCTGG ACCCTGAGGA TGAGGAGGAG 
CACACTCAGG AAGAGGACAG CAGTGGCAGT AACGAGGATG AGGATGATAG TCAGGATGAA 
GAGGAGGAGG AGGAGGAAGA TGAGGAAGAT GATCAGGAGG ATGATGAAGG TGAAGAGGGA 
GATGAAGACG ATGACGACGA TGGCTCTGAG ATGGAATTGG ATGAGGATTA TCCTGATATG 
AACGCTTCTC CCTTGGTCCG ATTTGAGCGC TTTGACCGGG AGGATGATCT CATCATTGAG 
TTTGACAACA TGTTCTCCAG TGCTACAGAC ATCCCCCCAT CCCCAGGAAA TATCCCTACC 
ACCCATCCAC TGATGGTGCG CCATGCAGAC CACAGTTCTC TGACACTGGG CAGTGGCTCT 
TCAACAACTC GTCTCACCCA GGGCATCGGG CGCAGTCAGA GGACCCTAAG GCAGCTGACG 
GCCAATACTG GCCACACCAT TCATGTTCAC TACCCTGGGA ATCGCCAGCC CAACCCTCCT 
CTTATACTGC AGAGGTTGCT TGGTCCCTCA GCTGCTGCTG ACATCCTTCA GCTGAGCAGC 
AGCCTTCCCC TACAAAGCCG GGGTCGGGCC CGCCTCCTGG TAGGCAACGA TGACGTCCAC 
ATCATCGCCC GTTCTGATGA TGAGCTGCTG GATGACTTTT TCCATGATCA GAGCACAGCT 
ACCAGCCAAG CAGGAACCCT GTCCAGCATC CCCACAGCCC TGACCCGCTG GACAGAAGAA 
TGCAAAGTTC TCGATGCTGA GAGCATGCAT GACTGTGTTT CAGTGGTTAA AGTGTCCATT 
GTCAATCACC TGGAATTCCT GAGGGATGAG GAGCTGGAAG AAAGGCGAGA GAAGCGCAGG 
AAACAACTGG CTGAGGAAGA AACAAAGATA ACTGATAAAG GCAAAGAAGA TAAGGAGAAC 
AGGGATCAGA GTGCACAGTG TACTGCATCT AAGTCAAATG ACTCCACTGA ACAGAATCTC 
TCAGATGGGA CGCCTATGCC TGACAGCTAC CCAACAACCC CATCTTCAAC TGATGCAGCT 
ACATCTGAGT CCAAGGAGAC CCTTGGCACT CTGCAATCCT CACAACAGCA ACCAACACTC 
CCAACCCCAC CAGCTTTGGG AGAGGTTCCT CAGGAGCTGC AGTCTCCAGC TGGAGAAGGG 
GGCAGCTCTA CACAGCTATT GATGCCTGTA GAGCCAGAGG AATTGGGTCC CACAAGGCCA 
AGTGGGGAAG CAGAAACAAC TCAGATGGAG TTATCCCCAG CTCCCACTAT AACCTCACTT 
TCCCCAGAGA GAGCTGAGGA TTCTGATGCA CTGACGGCTG TCAGCAGTCA GCTAGAAGGC 
TCTCCTATGG ATACAAGCAG CCTGGCTTCC TGTACCTTAG AGGAGGCTGT GGGTGACACT 
TCAGCAGCTG GCAGTTCTGA GCAGCCCAGA GCAGGCAGCT CCACTCCTGG GGATGCCCCA 
CCAGCTGTGG CGGAAGTGCA AGGCAGGAGT GATGGGTCAG GGGAATCTGC CCAGCCACCT 
GAGGACAGCT CCCCACCTGC ATCCTCTGAG AGCTCTTCCA CCAGAGATTC TGCCGTGGCC 
ATTTCTGGAG CAGATTCCCG AGGAATCCTA GAAGAGCCGT TGCCTTCAAC AAGCAGTGAA 
GAAGAAGATC CCCTTGCGGG TATCAGTCTC CCTGAAGGTG TGGACCCCTC TTTTCTGGCT 
GCCCTGCCTG ATGACATCCG TCGGGAAGTT CTACAGAACC AGCTAGGCAT TCGTCCACCA 
ACCCGGACTG CCCCCTCCAC AAATAGCTCA GCGCCTGCAG TGGTGGGGAA TCCTGGTGTG 
ACTGAAGTGA GCCCTGAGTT TCTGGCTGCC CTGCCTCCAG CCATTCAGGA GGAAGTACTG 
GCACAGCAGA GAGCTGAGCA GCAGCGACGA GAACTAGCAC AGAATGCCAG CTCAGACACC 
CCTATGGACC CTGTGACCTT CATCCAGACT CTGCCCTCAG ACCTGCGCCG TAGTGTCCTA 
GAGGATATGG AGGACAGTGT GTTAGCTGTG ATGCCACCTG ACATTGCAGC TGAGGCTCAA 
GCCCTGAGAC GAGAGCAAGA AGCCCGGCAG CGACAGCTCA TGCATGAGCG TCTGTTTGGG 
CACAGTAGCA CCTCCGCACT CTCTGCTATT CTCCGAAGCC CGGCTTTCAC CAGTCGCTTA 
AGTGGCAACC GTGGGGTCCA GTATACTCGC CTTGCTGTGC AGAGAGGTGG CACCTTCCAG 
ATGGGGGGTA GCAGCAGCCA TAACAGGCCT TCTGGCAGTA ATGTAGATAC TCTCCTCCGC 
CTCCGAGGAC GGCTCCTTCT GGACCACGAA GCCCTTTCTT GTCTCTTGGT CCTACTTTTT 
GTGGATGAGC CAAAGCTCAA TACTAGCCGT CTACACCGAG TACTGAGAAA TCTCTGCTAC 
CATGCCCAGA CCCGCCACTG GGTCATCCGC AGTCTGCTCT CCATCTTGCA GCGCAGCAGT 
GAGAGTGAGC TATGCATTGA AACACCCAAA CTCACTACAA GTGAGGAAAA GGGCAAAAAG 
TCGAGCAAGA GCTGTGGGTC AAGTAGCCAT GAGAACCGTC CCCTGGACCT GCTACACAAG 
ATGGAGTCAA AGAGCTCCAA CCAGCTTTCC TGGCTCTCAG TATCCATGGA TGCAGCCCTA 
GGCTGCAGGA CTAATATATT TCAGATCCAG CGTTCAGGGG GGCGTAAACA TACCGAGAAG 
CATGCAAGCG GTGGCTCCAC CGTCCACATC CATCCCCAAG CTGCTCCTGT TGTCTGCAGA 
CACGTTTTGG ATACACTCAT TCAATTGGCC AAGGTATTTC CCAGCCACTT CACACAGCAG 
CGGACCAAAG AAACAAACTG TGAGAGTGAT CGGGAAAGGG GCAATAAGGC CTGTAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATT TGCACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTCAG CCGGAAAGGC AAGAACTCCG TGAAGTCAGT GCCAGTGAGC 
GCTGGCGGTG AGGGGGAAAC CTCTCCATAC AGCCTCGAGG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACACCC AGTCATCCGC CGGAGCTCTC TCTTAACTGA GAAACTCCTC 
AGACTCCTTT CTCTCATCTC AATTGCTCTC CCAGAAAACA AGGTGTCAGA AGCACAGGCT 
AATTCTGGCA GCGGTGCTTC CTCCACCACC ACTGCCACCT CAACCACATC TACCACCACC 
ACCACTGCCG CCTCCACCAC GCCCACACCC CCTACTGCAC CCACCCCTGT CACTTCTGCT 
CCAGCCCTGG TTGCTGCCAC GGCTATTTCC ACCATTGTCG TAGCTGCTTC GACCACAGTG 
ACTACCCCCA CGACTGCTAC CACTACTGTT TCAATTTCTC CCACTACTAA GGGCAGCAAA 
TCTCCAGCGA AGGTGAGTGA TGGGGGCAGC AGCAGTACAG ACTTTAAGAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAGGAAGGCT TAGAGGATGC AGCCAACGTA CTACTGCAGC TCTCCCGGGG GGACTCTGGG 
ACCCGGGACA CTGTTCTCAA GCTGCTACTG AATGGAGCCC GCCATCTGGG TTATACCCTT 
TGTAAACAAA TAGGTACCCT GCTGGCCGAG CTGCGGGAAT ACAACCTCGA GCAGCAGCGG 
CGAGCCCAAT GTGAAACCCT CTCTCCTGAT GGCCTGCCTG AGGAGCAGCC ACAGACCACC 
AAGCTGAAGG GCAAAATGCA GAGCAGGTTT GACATGGCTG AGAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCTTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTCCGG 
GACGACACGC GCCGGGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTAGCC AGTCAGAGGC GTCTGTCCGG AGGGAGGAAT CACCCATGGA TGTGGACCAG 
CCATCTCCCA GTGCTCAAGA TACTCAATCC ATTGCCTCCG ATGGAACCCC ACAGGGGGAG 
AAGGAAAAGG AAGAAAGACC ACCTGAGTTA CCCCTGCTCA GCGAGCAGCT GAGTTTGGAC 
GAGCTGTGGG ACATGCTTGG GGAGTGTCTA AAGGAACTAG AGGAATCCCA TGACCAGCAT 
GCGGTGCTAG TGCTACAGCC TGCTGTCGAG GCCTTCTTTC TGGTCCATGC CACAGAGCGG 
GAGAGCAAGC CTCCTGTCCG AGACACCCGT GAGAGCCAGC TGGCACACAT CAAGGACGAG 
CCTCCTCCAC TCTCCCCTGC CCCCTTAACC CCAGCCACGC CTTCCTCCCT TGACCCATTC 
TTCTCCCGGG AGCCCTCATC TATGCACATC TCCTCAAGCC TGCCCCCTGA CACACAGAAG 
TTCCTTCGCT TTGCAGAGAC TCACCGCACT GTGTTAAACC AGATCCTACG GCAGTCCACG 
ACCCACCTTG CTGATGGGCC TTTTGCTGTC CTGGTAGACT ACATTCGTGT CCTCGACTTT 
GATGTCAAGC GCAAATATTT CCGCCAAGAG CTGGAGCGTT TAGATGAGGG GCTCCGGAAA 
GAAGACATGG CTGTGCATGT CCGTCGTGAC CATGTGTTTG AAGACTCCTA TCGTGAGCTG 
CATCGCAAAT CCCCCGAAGA AATGAAGAAT CGATTGTATA TAGTATTTGA AGGAGAAGAA 
GGGCAGGATG CTGGTGGGCT CCTGCGGGAG TGGTATATGA TCATCTCTCG AGAGATGTTT 
AACCCTATGT ATGCCTTGTT CCGTACCTCA CCTGGTGATC GAGTCACCTA CACCATCAAT 
CCATCTTCCC ACTGCAACCC CAACCACCTC AGCTACTTCA AGTTTGTCGG ACGCATTGTG 
GCCAAAGCTG TATATGACAA CCGTCTTCTG GAGTGCTACT TTACTCGATC CTTTTACAAA 
CACATCTTGG GCAAGTCAGT CAGATATACA GATATGGAGA GTGAAGATTA CCACTTCTAC 
CAAGGTCTGG TTTATCTGCT GGAAAATGAT GTCTCCACAC TAGGCTATGA CCTCACCTTC 
AGCACTGAGG TCCAAGAGTT TGGAGTTTGT GAAGTTCGTG ACCTCAAACC CAATGGGGCC 
AACATCTTGG TAACAGAGGA GAATAAGAAG GAGTATGTAC ACCTGGTATG CCAGATGAGA 
ATGACAGGAG CCATCCGCAA GCAGTTGGCG GCTTTCTTAG AAGGCTTCTA TGAGATCATT 
CCAAAGCGCC TCATTTCCAT CTTCACTGAG CAGGAGTTAG AGCTGCTTAT ATCAGGACTG 
CCCACCATTG ACATCGATGA TCTGAAATCC AACACTGAAT ACCACAAGTA CCAGTCCAAC 
TCTATTCAGA TCCAGTGGTT CTGGAGAGCA TTGCGTTCTT TCGATCAAGC TGACCGTGCC 
AAGTTCCTCC AGTTTGTCAC AGGTACTTCC AAGGTACCCC TGCAAGGCTT TGCTGCCCTC 
GAAGGCATGA ATGGCATTCA GAAGTTTCAG ATCCATCGAG ATGACAGGTC CACAGATCGC 
CTGCCTTCAG CTCACACATG TTTTAATCAG CTGGATCTGC CTGCCTATGA GAGCTTTGAG 
AAGCTCCGCC ACATGCTACT GTTGGCTATC CAGGAGTGCT CTGAAGGCTT TGGGCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884681.1
CDS	3018..16520
Translation	MDPFISRREQKCMEIGTPRKNGVLVLRSSEAVYHLTTPFQIVCLHLPLSKSKPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEDKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029192.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029192.1

ORF Insert Sequence:

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GTTGCAGATG TGGCTATGGA AAGCAGTCCA GGCTCATCCA TCTCTATGGA GCACAGGCTG 
GATGTTGAAT TAAGGGCATC AGGTTCCAGC AGCAGCACTA ACATCTCTTC TGGCCCCAGC 
CCTGGTCCCA GTCCCGGCCC CGGCACCGGC CCTGGCCCCG GCCCCGGCCC CGGCCCCGGC 
CCTGGCCCCG GCCCCGGCCC CGGTCCTGGT CCCGGCCCTG GCCCCGGCCC TGGCCCTGGC 
CCCCGTCCTG GAGTCCAGTG TATTCCACAA CGAGCAGCAC TTCTGAAATC CATGTTGAAT 
TTCCTCAAGA AGGCCATCCA AGACCCTGCT TTCTCAGATG GCATACGACA TGTGATGGAT 
GGTTCTCTGC CTACCTCCCT GAAACACATC ATCAGCAATG CAGAATACTA TGGCCCATCA 
CTCTTCCTCC TAGCTACTGA AGTGGTGACT GTGTTTGTAT TTCAAGAACC ATCACTGCTC 
TCCTCACTCC AGGACAATGG ATTGACAGAT GTCATGCTGC ATGCACTGCT TATCAAAGAT 
GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC CTCCCAAATG TATTCAGTGC ACTCTGTTTG 
AATGCCCGAG GTCTTCAGTC TTTTGTTCAG TGTCAGCCTT TTGAACGCCT CTTCAAAGTT 
CTTCTGTCTC CAGATTACCT CCCAGCCATG CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG 
GATACTGCAT CCAACCTGGG GAGTGCTGTC GATGAGCTCA TGAGACATCA GCCCACCCTT 
AAAACAGATG CAACGACTGC CATCATCAAG TTACTTGAAG AAATCTGTAA TCTTGGAAGG 
GACCCCAAAT ACATCTGTCA GAAGCCATCA ATCCAGAAGG CAGATGGCAC TGCCACTGCT 
CCTCCCCCAA GGTCTAATCA TGCCGCAGAA GAAGCCTCTA GTGAGGATGA GGAGGAAGAG 
GAAGTACAGG CCATGCAGAG CTTTAATTCT ACCCAGCAAA ATGAAACTGA GCCTAATCAG 
CAGGTTGTTG GTACAGAGGA ACGTATTCCT ATTCCCCTCA TGGATTACAT CCTTAATGTG 
ATGAAATTTG TGGAATCTAT TCTGAGCAAC AATACAACAG ATGACCACTG CCAGGAATTT 
GTGAATCAGA AAGGACTGTT GCCTTTGGTT ACCATTTTGG GTCTTCCCAA TCTGCCCATT 
GACTTTCCCA CATCTGCTGC CTGTCAGGCT GTTGCAGGTG TCTGCAAATC CATATTGACA 
CTGTCACATG AACCCAAAGT CCTTCAAGAG GGTCTCCTTC AGTTGGACTC CATCCTCTCC 
TCCCTGGAGC CCTTACACCG CCCCATTGAA TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA 
CTGGCTTGCG CAGGCAATGT TGCTGATGCT ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG 
CATGCACTCA CTGCTGCCCA TGCCTACATC ATGATGTTTG TTCATACTTG CAGAGTTGGA 
CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC CAGTGGGGCT CTCAATTGGG TCTGAGTGTT 
TTGAGCAAGC TGAGCCAGTT ATACTGTTCC CTGGTGTGGG AAAGCACTGT CCTCCTCTCT 
CTGTGTACCC CAAACAGCCT ACCATCTGGG TGTGAATTTG GCCAGGCAGA TATGCAGAAA 
CTGGTTCCAA AGGATGAGAA GGCAGGTACG ACCCAGGGCG GAAAAAGATC AGATGGGGAA 
CAGGATGGAG CAGCTGGAAG TATGGATGCT TCTACCCAGG GCTTATTAGA AGGCATTGGG 
CTAGATGGTG ACACATTGGC TCCCATGGAG ACAGATGAAC CTACTGCTTC AGACTCTAAG 
GGCAAATCTA AAATCACACC AGCAATGGCT GCCAGAATTA AGCAAATCAA GCCTTTGTTA 
TCAGCTTCCT CCAGATTAGG CCGAGCACTT GCTGAGCTAT TTGGACTTCT TGTTAAACTT 
TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG AGCCATCATG CTGCCAGCAC CACTACAGCA 
CCGACACCTG CCGCGCGATC AACAGCCTCA GCTCTCACTA AGCTCTTGAC TAAGGGGTTA 
TCTTGGCAGC CCCCACCATA TACACCTACT CCCCGATTCA GGCTGACATT CTTCATCTGT 
TCAGTTGGTT TCACATCCCC AATGCTGTTT GATGAGAGGA AGTATCCCTA CCACCTCATG 
CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC AATGCTCTTT TTGAAACTTT CAACTGGGCT 
CTGTCCATGG GAGGTAAAGT TCCTGTTTCT GAGGGATTGG AACACTCAGA CTTGCCTGAT 
GGCACAGGAG AATTCCTAGA TGCCTGGCTT ATGCTGGTGG AGAAGATGGT GAATCCCACC 
ACGGTGCTTG AATCTCCACA TTCGCTGCCT GCCAAATTGC CTGGAGGTGT CCAGAACTTT 
CCCCAGTTCA GTGCACTGCG CTTCCTTGTG GTAACTCAGA AAGCAGCCTT TACTTGCATC 
AAAAACTTAT GGAACCGGAA ACCCCTGAAG GTATATGGTG GACGAATGGC TGAATCGATG 
CTGGCCATTC TATGCCACAT CCTCCGAGGA GAACCTGTGA TTCGAGAGAG ACTAAGCAAG 
GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG 
GAACCTCAAG TCAACCAGCA ACAACTGCAA CAGCTCATGG ACATGGGCTT CACAAGGGAA 
CATGCAATGG AGGCACTGTT GAACACCAGC ACCATGGAGC AGGCCACAGA GTACCTTTTA 
ACCCACCCTC CTCCAATCAT GGGAGGAGTT GTTCGGGATC TCAGCATGTC TGAAGAGGAC 
CAGATGATGA GAGCAATTGC TATGTCTCTG GGACAGGATA TTCCAATGGA TCAAAGGGCA 
GAGTCACCTG AGGAAGTTGC TTGCCGGAAG GAGGAAGAGG AACGGAAAGC TCGGGAAAAG 
CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG AAGTTCCAGG ATGCTGACCC GTTGGAACAA 
GATGAGCTCC ACACTTTCAC AGATACTATG TTGCCAGGCT GCTTCCACCT TCTTGATGAG 
CTGCCAGACA CAGTATACCG TGTGTGTGAC CTGATCATGA CAGCAATCAA ACGTAATGGA 
GCAGATTATC GTGACATGAT TCTGAAGCAA GTAGTCAATC AGGTGTGGGA AGCTGCTGAT 
GTATTGATCA AAGCTGCTCT TCCCCTGACA ACAAGTGACA CAAAAACCGT GTCAGAGTGG 
ATAAGTCAGA TGGCCACACT GCCCCAGGCC TCCAATTTGG CTACTAGAAT CTTGCTTTTA 
ACGCTACTTT TTGAGGAGTT GAAGCTACCT TGTGCTTGGG TGGTTGAATC AAGTGGCATC 
CTTAATGTCC TAATCAAACT CTTGGAAGTG GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG 
CAGAAGGAAG TCCAGACCCC AAAGTGGATC ACACCAGTGT TGCTCCTGAT TGATTTCTAT 
GAAAAGACAG CCATCTCCTC AAAAAGGAGA GCCCAGATGA CTAAGTACCT GCAATCCAAC 
AGCAACAACT GGCGCTGGTT TGATGATCGC TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC 
AACAATAGCA CTATTGATTC TGCCTGGAAA TCTGGAGAGA CAAGCGTGCG ATTCACTGCA 
GGCCGAAGAA GATACACGGT CCAATTCACT ACAATGGTGC AGGTTAATGA GGAAACAGGG 
AACCGACGCC CTGTGATGCT GACTCTCCTC AGGGTACCTC GGCTGAATAA AAATTCAAAA 
AACAGCAATG GACAGGAACT AGAGAAGACG CTGGAAGAAA GCAAAGAAAT GGATATCAAA 
CGTAAAGAAA ATAAAGGCAA TGATACCCCT TTGGCCCTAG AGAGTACAAA CACTGAAAAG 
GAGACAAGCC TGGAGGAAAC AAAAATCGGG GAGATCCTGA TCCAGGGCTT GACAGAAGAT 
ATGGTGACTG TTTTAATCCG GGCCTGCGTG AGCATGCTGG GAGTCCCTGT GGACCCAGAT 
ACTTTGCATG CCACCCTTCG TCTCTGTCTG AGGCTCACCC GGGACCACAA ATATGCCATG 
ATGTTTGCAG AACTGAAGAG TACCCGCATG ATCTTGAATT TGACCCAGAG CTCAGGCTTC 
AATGGGTTTA CTCCCCTGGT CACCCTTCTC TTAAGACACA TCATTGAGGA CCCCTGTACC 
CTTCGTCATA CCATGGAAAA GGTTGTTCGC TCAGCAGCTA CAAGTGGAGC TGGTAGCACT 
ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC TCTCGGGAGA TCAACTACAT CCTTCGTGTC 
CTTGGGCCAG CCGCATGCCG CAATCCAGAC ATATTCACAG AAGTGGCCAA CTGCTGTATC 
CGCATCGCCC TTCCTGCCCC TCGAGGCTCA GGAACTGCTT CAGATGATGA ATTTGAGAAT 
CTTAGAATTA AAGGCCCTAA TGCTGTACAG CTGGTGAAGA CCACCCCTTT GAAGCCCTCA 
CCTCTGCCTG TCATCCCTGA TACTATCAAG GAAGTGATCT ATGATATGCT GAATGCTCTG 
GCTGCATACC ATGCTCCAGA GGAAGATAAA TCTGATCCTA AACCTGGGGT TATGACCCAA 
GAGGTTGGCC AGCTCCTGCA AGACATGGGT GATGATGTAT ACCAGCAGTA CCGGTCACTT 
ACGCGTCAGA GCAGTGACTT TGATACGCAG TCAGGTTTTT CCATTAATAG TCAGGTCTTT 
GCTGCAGATG GTGCCTCCAC TGAGACTTCC GCATCTGGGA CCTCCCAAGG AGAGGCTTCA 
ACTCCAGAGG AGTCTCGAGA TGGGAAGAAA GATAAAGAAG GGGACCGGGC CTCTGAGGAA 
GGCAAACAGA AAGGCAAGGG CAGCAAACCT TTAATGCCTA CCTCCACTAT CCTTCGTCTT 
CTGGCAGAGT TGGTGAGGTC CTATGTTGGT ATTGCTACCC TGATTGCCAA CTACAGCTAC 
ACTGTGGGCC AGTCTGAACT GATCAAAGAG GACTGCAGTG TGCTAGCTTT TGTTCTGGAC 
CACCTGCTCC CACATACCCA GAATGCAGAA GACAAGGACA CCCCTGCCTT GGCCCGCCTG 
TTCCTCGCAA GCCTGGCTGC TGCAGGGAGT GGCACAGATG CCCAGGTGGC CCTAGTGAAT 
GAAGTAAAAG CAGCCCTTGG ACGGGCACTG GCTATGGCTG AGAGTACAGA GAAACATGCC 
AGGCTTCAGG CAGTGATGTG TATCATCAGT ACTATCATGG AGTCCTGCCC CTCCACCTCC 
AGCTTCTACA GCAGTGCCAC AGCGAAGACC CAGCACAATG GCATGAACAA CATCATTCGG 
CTTTTCCTGA AGAAGGGACT GGTTAATGAC CTGGCCAGAG TACCTCACAG CTTAGACCTG 
TCCAGTCCCA ACATGGCCAA CACAGTCAAT GCTGCTCTGA AGCCTTTGGA AACACTTTCC 
CGGATTGTGA ACCAGCCCAG TAGCCTTTTT GGCAGCAAGA GTGCTTCTAG CAAGAACAAG 
TCTGAGCAGG ATGCCCAAGG AGCCTCTCAA GATTCCAGTA GCAACCAGCA GGACCCAGGC 
GAGCCTGGGG AAGCAGAAGT GCAGGAGGAG GATCATGATG TCACTCAGAC AGAGGTGGCA 
GATGGGGATA TCATGGATGG GGAGGCTGAA ACCGACTCAG TGGTGATTGC TGGGCAGCCT 
GAGGTGCTCA GTTCACAAGA GATGCAGGTT GAGAATGAGC TGGAGGACCT GATAGATGAG 
TTGCTTGAGA GGGATGGCGG ATCTGGGAAC AGTACAATTA TAGTGAGCAG AAGTGGAGAG 
GATGAATCAC AAGAGGACGT GCTGATGGAT GAAGCTCCTT CCAACCTCAG CCAAGCTTCC 
ACCTTGCAGG CCAACCGAGA AGATTCCATG AATATCCTGG ACCCTGAGGA TGAGGAGGAG 
CACACTCAGG AAGAGGACAG CAGTGGCAGT AACGAGGATG AGGATGATAG TCAGGATGAA 
GAGGAGGAGG AGGAGGAAGA TGAGGAAGAT GATCAGGAGG ATGATGAAGG TGAAGAGGGA 
GATGAAGACG ATGACGACGA TGGCTCTGAG ATGGAATTGG ATGAGGATTA TCCTGATATG 
AACGCTTCTC CCTTGGTCCG ATTTGAGCGC TTTGACCGGG AGGATGATCT CATCATTGAG 
TTTGACAACA TGTTCTCCAG TGCTACAGAC ATCCCCCCAT CCCCAGGAAA TATCCCTACC 
ACCCATCCAC TGATGGTGCG CCATGCAGAC CACAGTTCTC TGACACTGGG CAGTGGCTCT 
TCAACAACTC GTCTCACCCA GGGCATCGGG CGCAGTCAGA GGACCCTAAG GCAGCTGACG 
GCCAATACTG GCCACACCAT TCATGTTCAC TACCCTGGGA ATCGCCAGCC CAACCCTCCT 
CTTATACTGC AGAGGTTGCT TGGTCCCTCA GCTGCTGCTG ACATCCTTCA GCTGAGCAGC 
AGCCTTCCCC TACAAAGCCG GGGTCGGGCC CGCCTCCTGG TAGGCAACGA TGACGTCCAC 
ATCATCGCCC GTTCTGATGA TGAGCTGCTG GATGACTTTT TCCATGATCA GAGCACAGCT 
ACCAGCCAAG CAGGAACCCT GTCCAGCATC CCCACAGCCC TGACCCGCTG GACAGAAGAA 
TGCAAAGTTC TCGATGCTGA GAGCATGCAT GACTGTGTTT CAGTGGTTAA AGTGTCCATT 
GTCAATCACC TGGAATTCCT GAGGGATGAG GAGCTGGAAG AAAGGCGAGA GAAGCGCAGG 
AAACAACTGG CTGAGGAAGA AACAAAGATA ACTGATAAAG GCAAAGAAGA TAAGGAGAAC 
AGGGATCAGA GTGCACAGTG TACTGCATCT AAGTCAAATG ACTCCACTGA ACAGAATCTC 
TCAGATGGGA CGCCTATGCC TGACAGCTAC CCAACAACCC CATCTTCAAC TGATGCAGCT 
ACATCTGAGT CCAAGGAGAC CCTTGGCACT CTGCAATCCT CACAACAGCA ACCAACACTC 
CCAACCCCAC CAGCTTTGGG AGAGGTTCCT CAGGAGCTGC AGTCTCCAGC TGGAGAAGGG 
GGCAGCTCTA CACAGCTATT GATGCCTGTA GAGCCAGAGG AATTGGGTCC CACAAGGCCA 
AGTGGGGAAG CAGAAACAAC TCAGATGGAG TTATCCCCAG CTCCCACTAT AACCTCACTT 
TCCCCAGAGA GAGCTGAGGA TTCTGATGCA CTGACGGCTG TCAGCAGTCA GCTAGAAGGC 
TCTCCTATGG ATACAAGCAG CCTGGCTTCC TGTACCTTAG AGGAGGCTGT GGGTGACACT 
TCAGCAGCTG GCAGTTCTGA GCAGCCCAGA GCAGGCAGCT CCACTCCTGG GGATGCCCCA 
CCAGCTGTGG CGGAAGTGCA AGGCAGGAGT GATGGGTCAG GGGAATCTGC CCAGCCACCT 
GAGGACAGCT CCCCACCTGC ATCCTCTGAG AGCTCTTCCA CCAGAGATTC TGCCGTGGCC 
ATTTCTGGAG CAGATTCCCG AGGAATCCTA GAAGAGCCGT TGCCTTCAAC AAGCAGTGAA 
GAAGAAGATC CCCTTGCGGG TATCAGTCTC CCTGAAGGTG TGGACCCCTC TTTTCTGGCT 
GCCCTGCCTG ATGACATCCG TCGGGAAGTT CTACAGAACC AGCTAGGCAT TCGTCCACCA 
ACCCGGACTG CCCCCTCCAC AAATAGCTCA GCGCCTGCAG TGGTGGGGAA TCCTGGTGTG 
ACTGAAGTGA GCCCTGAGTT TCTGGCTGCC CTGCCTCCAG CCATTCAGGA GGAAGTACTG 
GCACAGCAGA GAGCTGAGCA GCAGCGACGA GAACTAGCAC AGAATGCCAG CTCAGACACC 
CCTATGGACC CTGTGACCTT CATCCAGACT CTGCCCTCAG ACCTGCGCCG TAGTGTCCTA 
GAGGATATGG AGGACAGTGT GTTAGCTGTG ATGCCACCTG ACATTGCAGC TGAGGCTCAA 
GCCCTGAGAC GAGAGCAAGA AGCCCGGCAG CGACAGCTCA TGCATGAGCG TCTGTTTGGG 
CACAGTAGCA CCTCCGCACT CTCTGCTATT CTCCGAAGCC CGGCTTTCAC CAGTCGCTTA 
AGTGGCAACC GTGGGGTCCA GTATACTCGC CTTGCTGTGC AGAGAGGTGG CACCTTCCAG 
ATGGGGGGTA GCAGCAGCCA TAACAGGCCT TCTGGCAGTA ATGTAGATAC TCTCCTCCGC 
CTCCGAGGAC GGCTCCTTCT GGACCACGAA GCCCTTTCTT GTCTCTTGGT CCTACTTTTT 
GTGGATGAGC CAAAGCTCAA TACTAGCCGT CTACACCGAG TACTGAGAAA TCTCTGCTAC 
CATGCCCAGA CCCGCCACTG GGTCATCCGC AGTCTGCTCT CCATCTTGCA GCGCAGCAGT 
GAGAGTGAGC TATGCATTGA AACACCCAAA CTCACTACAA GTGAGGAAAA GGGCAAAAAG 
TCGAGCAAGA GCTGTGGGTC AAGTAGCCAT GAGAACCGTC CCCTGGACCT GCTACACAAG 
ATGGAGTCAA AGAGCTCCAA CCAGCTTTCC TGGCTCTCAG TATCCATGGA TGCAGCCCTA 
GGCTGCAGGA CTAATATATT TCAGATCCAG CGTTCAGGGG GGCGTAAACA TACCGAGAAG 
CATGCAAGCG GTGGCTCCAC CGTCCACATC CATCCCCAAG CTGCTCCTGT TGTCTGCAGA 
CACGTTTTGG ATACACTCAT TCAATTGGCC AAGGTATTTC CCAGCCACTT CACACAGCAG 
CGGACCAAAG AAACAAACTG TGAGAGTGAT CGGGAAAGGG GCAATAAGGC CTGTAGCCCA 
TGCTCCTCAC AGTCCTCCAG CAGTGGCATT TGCACAGACT TCTGGGACTT ATTGGTAAAA 
CTGGACAACA TGAATGTCAG CCGGAAAGGC AAGAACTCCG TGAAGTCAGT GCCAGTGAGC 
GCTGGCGGTG AGGGGGAAAC CTCTCCATAC AGCCTCGAGG CCTCTCCACT GGGGCAGCTC 
ATGAACATGT TGTCACACCC AGTCATCCGC CGGAGCTCTC TCTTAACTGA GAAACTCCTC 
AGACTCCTTT CTCTCATCTC AATTGCTCTC CCAGAAAACA AGGTGTCAGA AGCACAGGCT 
AATTCTGGCA GCGGTGCTTC CTCCACCACC ACTGCCACCT CAACCACATC TACCACCACC 
ACCACTGCCG CCTCCACCAC GCCCACACCC CCTACTGCAC CCACCCCTGT CACTTCTGCT 
CCAGCCCTGG TTGCTGCCAC GGCTATTTCC ACCATTGTCG TAGCTGCTTC GACCACAGTG 
ACTACCCCCA CGACTGCTAC CACTACTGTT TCAATTTCTC CCACTACTAA GGGCAGCAAA 
TCTCCAGCGA AGGTGAGTGA TGGGGGCAGC AGCAGTACAG ACTTTAAGAT GGTGTCCTCT 
GGCCTCACTG AAAACCAGCT ACAGCTCTCT GTAGAGGTGT TGACATCCCA CTCTTGTTCT 
GAGGAAGGCT TAGAGGATGC AGCCAACGTA CTACTGCAGC TCTCCCGGGG GGACTCTGGG 
ACCCGGGACA CTGTTCTCAA GCTGCTACTG AATGGAGCCC GCCATCTGGG TTATACCCTT 
TGTAAACAAA TAGGTACCCT GCTGGCCGAG CTGCGGGAAT ACAACCTCGA GCAGCAGCGG 
CGAGCCCAAT GTGAAACCCT CTCTCCTGAT GGCCTGCCTG AGGAGCAGCC ACAGACCACC 
AAGCTGAAGG GCAAAATGCA GAGCAGGTTT GACATGGCTG AGAATGTGGT AATTGTGGCA 
TCTCAGAAGC GACCTTTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA 
TCCAAGACAT CTACCCAGAA GTTCTTCTTG AGGGTACTAC AGGTCATCAT CCAGCTCCGG 
GACGACACGC GCCGGGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCCTCCGGT 
TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC 
ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG 
GAGTCTAGCC AGTCAGAGGC GTCTGTCCGG AGGGAGGAAT CACCCATGGA TGTGGACCAG 
CCATCTCCCA GTGCTCAAGA TACTCAATCC ATTGCCTCCG ATGGAACCCC ACAGGGGGAG 
AAGGAAAAGG AAGAAAGACC ACCTGAGTTA CCCCTGCTCA GCGAGCAGCT GAGTTTGGAC 
GAGCTGTGGG ACATGCTTGG GGAGTGTCTA AAGGAACTAG AGGAATCCCA TGACCAGCAT 
GCGGTGCTAG TGCTACAGCC TGCTGTCGAG GCCTTCTTTC TGGTCCATGC CACAGAGCGG 
GAGAGCAAGC CTCCTGTCCG AGACACCCGT GAGAGCCAGC TGGCACACAT CAAGGACGAG 
CCTCCTCCAC TCTCCCCTGC CCCCTTAACC CCAGCCACGC CTTCCTCCCT TGACCCATTC 
TTCTCCCGGG AGCCCTCATC TATGCACATC TCCTCAAGCC TGCCCCCTGA CACACAGAAG 
TTCCTTCGCT TTGCAGAGAC TCACCGCACT GTGTTAAACC AGATCCTACG GCAGTCCACG 
ACCCACCTTG CTGATGGGCC TTTTGCTGTC CTGGTAGACT ACATTCGTGT CCTCGACTTT 
GATGTCAAGC GCAAATATTT CCGCCAAGAG CTGGAGCGTT TAGATGAGGG GCTCCGGAAA 
GAAGACATGG CTGTGCATGT CCGTCGTGAC CATGTGTTTG AAGACTCCTA TCGTGAGCTG 
CATCGCAAAT CCCCCGAAGA AATGAAGAAT CGATTGTATA TAGTATTTGA AGGAGAAGAA 
GGGCAGGATG CTGGTGGGCT CCTGCGGGAG TGGTATATGA TCATCTCTCG AGAGATGTTT 
AACCCTATGT ATGCCTTGTT CCGTACCTCA CCTGGTGATC GAGTCACCTA CACCATCAAT 
CCATCTTCCC ACTGCAACCC CAACCACCTC AGCTACTTCA AGTTTGTCGG ACGCATTGTG 
GCCAAAGCTG TATATGACAA CCGTCTTCTG GAGTGCTACT TTACTCGATC CTTTTACAAA 
CACATCTTGG GCAAGTCAGT CAGATATACA GATATGGAGA GTGAAGATTA CCACTTCTAC 
CAAGGTCTGG TTTATCTGCT GGAAAATGAT GTCTCCACAC TAGGCTATGA CCTCACCTTC 
AGCACTGAGG TCCAAGAGTT TGGAGTTTGT GAAGTTCGTG ACCTCAAACC CAATGGGGCC 
AACATCTTGG TAACAGAGGA GAATAAGAAG GAGTATGTAC ACCTGGTATG CCAGATGAGA 
ATGACAGGAG CCATCCGCAA GCAGTTGGCG GCTTTCTTAG AAGGCTTCTA TGAGATCATT 
CCAAAGCGCC TCATTTCCAT CTTCACTGAG CAGGAGTTAG AGCTGCTTAT ATCAGGACTG 
CCCACCATTG ACATCGATGA TCTGAAATCC AACACTGAAT ACCACAAGTA CCAGTCCAAC 
TCTATTCAGA TCCAGTGGTT CTGGAGAGCA TTGCGTTCTT TCGATCAAGC TGACCGTGCC 
AAGTTCCTCC AGTTTGTCAC AGGTACTTCC AAGGTACCCC TGCAAGGCTT TGCTGCCCTC 
GAAGGCATGA ATGGCATTCA GAAGTTTCAG ATCCATCGAG ATGACAGGTC CACAGATCGC 
CTGCCTTCAG CTCACACATG TTTTAATCAG CTGGATCTGC CTGCCTATGA GAGCTTTGAG 
AAGCTCCGCC ACATGCTACT GTTGGCTATC CAGGAGTGCT CTGAAGGCTT TGGGCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029193.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884682.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X3, mRNA.

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CloneID	OHu99114
Clone ID Related Accession (Same CDS sequence)	XM_017029193.1
Accession Version	XM_017029193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTATTC CAATGCCTTG CAGGAATCAG CAAACAGTAT CTTGTATAAT 
GGCTTGATAG AGGAGTTGGT AGATGTCCTT CAGATAACGG ATAAGCAGCT TATGGAGATT 
AAAGCAGCTT CTTTACGAAC ATTAACATCA ATTGTCCACT TGGAGAGAAC TCCCAAACTC 
AGCAGTATTA TTGACTGTAC TGGAACTGCC TCCTACCATG GATTTTTGCC AGTGCTTGTA 
AGGAACTGTA TCCAGGCCAT GATTGATCCT TCCATGGATC CATACCCTCA CCAGTTTGCC 
ACTGCTCTCT TCTCTTTTTT ATACCATCTG GCCAGCTACG ATGCTGGTGG TGAAGCCTTG 
GTCTCCTGTG GAATGATGGA AGCCTTATTG AAGGTCATAA AGTTTCTTGG CGATGAACAG 
GACCAGATAA CATTTGTCAC CAGAGCCGTC AGAGTGGTTG ACCTTATCAC CAACCTGGAT 
ATGGCAGCTT TTCAATCCCA TAGTGGACTT TCTATCTTCA TTTATAGACT TGAGCATGAA 
GTAGATTTGT GCCGAAAAGA ATGTCCGTTT GTGATCAAGC CAAAGATCCA GAGACCCAAT 
ACTACACAAG AAGGAGAGGA AATGGAAACT GATATGGATG TTGCAGATGT GGCTATGGAA 
AGCAGTCCAG GCTCATCCAT CTCTATGGAG CACAGGCTGG ATGTTGAATT AAGGGCATCA 
GGTTCCAGCA GCAGCACTAA CATCTCTTCT GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC 
GGCACCGGCC CTGGCCCCGG CCCCGGCCCC GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC 
GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT 
ATTCCACAAC GAGCAGCACT TCTGAAATCC ATGTTGAATT TCCTCAAGAA GGCCATCCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTCTGCC TACCTCCCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGCCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAACCA TCACTGCTCT CCTCACTCCA GGACAATGGA 
TTGACAGATG TCATGCTGCA TGCACTGCTT ATCAAAGATG TTCCTGCTAC CCGTGAAGTC 
CTTGGCTCCC TCCCAAATGT ATTCAGTGCA CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTTCAGT GTCAGCCTTT TGAACGCCTC TTCAAAGTTC TTCTGTCTCC AGATTACCTC 
CCAGCCATGC GGAGGAGGAG AAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTCG ATGAGCTCAT GAGACATCAG CCCACCCTTA AAACAGATGC AACGACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGGG ACCCCAAATA CATCTGTCAG 
AAGCCATCAA TCCAGAAGGC AGATGGCACT GCCACTGCTC CTCCCCCAAG GTCTAATCAT 
GCCGCAGAAG AAGCCTCTAG TGAGGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTA CCCAGCAAAA TGAAACTGAG CCTAATCAGC AGGTTGTTGG TACAGAGGAA 
CGTATTCCTA TTCCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
CTGAGCAACA ATACAACAGA TGACCACTGC CAGGAATTTG TGAATCAGAA AGGACTGTTG 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACAC TGTCACATGA ACCCAAAGTC 
CTTCAAGAGG GTCTCCTTCA GTTGGACTCC ATCCTCTCCT CCCTGGAGCC CTTACACCGC 
CCCATTGAAT CCCCTGGGGG CTCAGTGTTG TTGCGAGAAC TGGCTTGCGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC CCAGGCCACA CCTCTGCTGC ATGCACTCAC TGCTGCCCAT 
GCCTACATCA TGATGTTTGT TCATACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCCATC 
TCCGTAAACC AGTGGGGCTC TCAATTGGGT CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA 
TACTGTTCCC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TGTGTACCCC AAACAGCCTA 
CCATCTGGGT GTGAATTTGG CCAGGCAGAT ATGCAGAAAC TGGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGGGAAC AGGATGGAGC AGCTGGAAGT 
ATGGATGCTT CTACCCAGGG CTTATTAGAA GGCATTGGGC TAGATGGTGA CACATTGGCT 
CCCATGGAGA CAGATGAACC TACTGCTTCA GACTCTAAGG GCAAATCTAA AATCACACCA 
GCAATGGCTG CCAGAATTAA GCAAATCAAG CCTTTGTTAT CAGCTTCCTC CAGATTAGGC 
CGAGCACTTG CTGAGCTATT TGGACTTCTT GTTAAACTTT GTGTGGGATC TCCTGTCCGC 
CAGAGAAGGA GCCATCATGC TGCCAGCACC ACTACAGCAC CGACACCTGC CGCGCGATCA 
ACAGCCTCAG CTCTCACTAA GCTCTTGACT AAGGGGTTAT CTTGGCAGCC CCCACCATAT 
ACACCTACTC CCCGATTCAG GCTGACATTC TTCATCTGTT CAGTTGGTTT CACATCCCCA 
ATGCTGTTTG ATGAGAGGAA GTATCCCTAC CACCTCATGC TGCAAAAATT TCTCTGCTCC 
GGAGGCCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TGTCCATGGG AGGTAAAGTT 
CCTGTTTCTG AGGGATTGGA ACACTCAGAC TTGCCTGATG GCACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTGGTGGA GAAGATGGTG AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGCTGCCTG CCAAATTGCC TGGAGGTGTC CAGAACTTTC CCCAGTTCAG TGCACTGCGC 
TTCCTTGTGG TAACTCAGAA AGCAGCCTTT ACTTGCATCA AAAACTTATG GAACCGGAAA 
CCCCTGAAGG TATATGGTGG ACGAATGGCT GAATCGATGC TGGCCATTCT ATGCCACATC 
CTCCGAGGAG AACCTGTGAT TCGAGAGAGA CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA 
GAAGAGGATA CAGGGCAAGA GGAAGGTGGC TCCCGCCGGG AACCTCAAGT CAACCAGCAA 
CAACTGCAAC AGCTCATGGA CATGGGCTTC ACAAGGGAAC ATGCAATGGA GGCACTGTTG 
AACACCAGCA CCATGGAGCA GGCCACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATG 
GGAGGAGTTG TTCGGGATCT CAGCATGTCT GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA 
TGTCTAGAGA AGTTCCAGGA TGCTGACCCG TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACTATGT TGCCAGGCTG CTTCCACCTT CTTGATGAGC TGCCAGACAC AGTATACCGT 
GTGTGTGACC TGATCATGAC AGCAATCAAA CGTAATGGAG CAGATTATCG TGACATGATT 
CTGAAGCAAG TAGTCAATCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCCCTGACAA CAAGTGACAC AAAAACCGTG TCAGAGTGGA TAAGTCAGAT GGCCACACTG 
CCCCAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CGCTACTTTT TGAGGAGTTG 
AAGCTACCTT GTGCTTGGGT GGTTGAATCA AGTGGCATCC TTAATGTCCT AATCAAACTC 
TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA GCCAAGGAGC AGAAGGAAGT CCAGACCCCA 
AAGTGGATCA CACCAGTGTT GCTCCTGATT GATTTCTATG AAAAGACAGC CATCTCCTCA 
AAAAGGAGAG CCCAGATGAC TAAGTACCTG CAATCCAACA GCAACAACTG GCGCTGGTTT 
GATGATCGCT CTGGGCGTTG GTGTAGTTAC AGTGCAAGCA ACAATAGCAC TATTGATTCT 
GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA TTCACTGCAG GCCGAAGAAG ATACACGGTC 
CAATTCACTA CAATGGTGCA GGTTAATGAG GAAACAGGGA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTACCTCG GCTGAATAAA AATTCAAAAA ACAGCAATGG ACAGGAACTA 
GAGAAGACGC TGGAAGAAAG CAAAGAAATG GATATCAAAC GTAAAGAAAA TAAAGGCAAT 
GATACCCCTT TGGCCCTAGA GAGTACAAAC ACTGAAAAGG AGACAAGCCT GGAGGAAACA 
AAAATCGGGG AGATCCTGAT CCAGGGCTTG ACAGAAGATA TGGTGACTGT TTTAATCCGG 
GCCTGCGTGA GCATGCTGGG AGTCCCTGTG GACCCAGATA CTTTGCATGC CACCCTTCGT 
CTCTGTCTGA GGCTCACCCG GGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACCCGCATGA TCTTGAATTT GACCCAGAGC TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC 
ACCCTTCTCT TAAGACACAT CATTGAGGAC CCCTGTACCC TTCGTCATAC CATGGAAAAG 
GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT GGTAGCACTA CCTCTGGTGT TGTGTCTGGC 
AGCCTCGGCT CTCGGGAGAT CAACTACATC CTTCGTGTCC TTGGGCCAGC CGCATGCCGC 
AATCCAGACA TATTCACAGA AGTGGCCAAC TGCTGTATCC GCATCGCCCT TCCTGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGCCCTAAT 
GCTGTACAGC TGGTGAAGAC CACCCCTTTG AAGCCCTCAC CTCTGCCTGT CATCCCTGAT 
ACTATCAAGG AAGTGATCTA TGATATGCTG AATGCTCTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGATA AATCTGATCC TAAACCTGGG GTTATGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGATGATGT ATACCAGCAG TACCGGTCAC TTACGCGTCA GAGCAGTGAC 
TTTGATACGC AGTCAGGTTT TTCCATTAAT AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC 
ACTGAGACTT CCGCATCTGG GACCTCCCAA GGAGAGGCTT CAACTCCAGA GGAGTCTCGA 
GATGGGAAGA AAGATAAAGA AGGGGACCGG GCCTCTGAGG AAGGCAAACA GAAAGGCAAG 
GGCAGCAAAC CTTTAATGCC TACCTCCACT ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG 
TCCTATGTTG GTATTGCTAC CCTGATTGCC AACTACAGCT ACACTGTGGG CCAGTCTGAA 
CTGATCAAAG AGGACTGCAG TGTGCTAGCT TTTGTTCTGG ACCACCTGCT CCCACATACC 
CAGAATGCAG AAGACAAGGA CACCCCTGCC TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT 
GCTGCAGGGA GTGGCACAGA TGCCCAGGTG GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT 
GGACGGGCAC TGGCTATGGC TGAGAGTACA GAGAAACATG CCAGGCTTCA GGCAGTGATG 
TGTATCATCA GTACTATCAT GGAGTCCTGC CCCTCCACCT CCAGCTTCTA CAGCAGTGCC 
ACAGCGAAGA CCCAGCACAA TGGCATGAAC AACATCATTC GGCTTTTCCT GAAGAAGGGA 
CTGGTTAATG ACCTGGCCAG AGTACCTCAC AGCTTAGACC TGTCCAGTCC CAACATGGCC 
AACACAGTCA ATGCTGCTCT GAAGCCTTTG GAAACACTTT CCCGGATTGT GAACCAGCCC 
AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT AGCAAGAACA AGTCTGAGCA GGATGCCCAA 
GGAGCCTCTC AAGATTCCAG TAGCAACCAG CAGGACCCAG GCGAGCCTGG GGAAGCAGAA 
GTGCAGGAGG AGGATCATGA TGTCACTCAG ACAGAGGTGG CAGATGGGGA TATCATGGAT 
GGGGAGGCTG AAACCGACTC AGTGGTGATT GCTGGGCAGC CTGAGGTGCT CAGTTCACAA 
GAGATGCAGG TTGAGAATGA GCTGGAGGAC CTGATAGATG AGTTGCTTGA GAGGGATGGC 
GGATCTGGGA ACAGTACAAT TATAGTGAGC AGAAGTGGAG AGGATGAATC ACAAGAGGAC 
GTGCTGATGG ATGAAGCTCC TTCCAACCTC AGCCAAGCTT CCACCTTGCA GGCCAACCGA 
GAAGATTCCA TGAATATCCT GGACCCTGAG GATGAGGAGG AGCACACTCA GGAAGAGGAC 
AGCAGTGGCA GTAACGAGGA TGAGGATGAT AGTCAGGATG AAGAGGAGGA GGAGGAGGAA 
GATGAGGAAG ATGATCAGGA GGATGATGAA GGTGAAGAGG GAGATGAAGA CGATGACGAC 
GATGGCTCTG AGATGGAATT GGATGAGGAT TATCCTGATA TGAACGCTTC TCCCTTGGTC 
CGATTTGAGC GCTTTGACCG GGAGGATGAT CTCATCATTG AGTTTGACAA CATGTTCTCC 
AGTGCTACAG ACATCCCCCC ATCCCCAGGA AATATCCCTA CCACCCATCC ACTGATGGTG 
CGCCATGCAG ACCACAGTTC TCTGACACTG GGCAGTGGCT CTTCAACAAC TCGTCTCACC 
CAGGGCATCG GGCGCAGTCA GAGGACCCTA AGGCAGCTGA CGGCCAATAC TGGCCACACC 
ATTCATGTTC ACTACCCTGG GAATCGCCAG CCCAACCCTC CTCTTATACT GCAGAGGTTG 
CTTGGTCCCT CAGCTGCTGC TGACATCCTT CAGCTGAGCA GCAGCCTTCC CCTACAAAGC 
CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC GATGACGTCC ACATCATCGC CCGTTCTGAT 
GATGAGCTGC TGGATGACTT TTTCCATGAT CAGAGCACAG CTACCAGCCA AGCAGGAACC 
CTGTCCAGCA TCCCCACAGC CCTGACCCGC TGGACAGAAG AATGCAAAGT TCTCGATGCT 
GAGAGCATGC ATGACTGTGT TTCAGTGGTT AAAGTGTCCA TTGTCAATCA CCTGGAATTC 
CTGAGGGATG AGGAGCTGGA AGAAAGGCGA GAGAAGCGCA GGAAACAACT GGCTGAGGAA 
GAAACAAAGA TAACTGATAA AGGCAAAGAA GATAAGGAGA ACAGGGATCA GAGTGCACAG 
TGTACTGCAT CTAAGTCAAA TGACTCCACT GAACAGAATC TCTCAGATGG GACGCCTATG 
CCTGACAGCT ACCCAACAAC CCCATCTTCA ACTGATGCAG CTACATCTGA GTCCAAGGAG 
ACCCTTGGCA CTCTGCAATC CTCACAACAG CAACCAACAC TCCCAACCCC ACCAGCTTTG 
GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA GCTGGAGAAG GGGGCAGCTC TACACAGCTA 
TTGATGCCTG TAGAGCCAGA GGAATTGGGT CCCACAAGGC CAAGTGGGGA AGCAGAAACA 
ACTCAGATGG AGTTATCCCC AGCTCCCACT ATAACCTCAC TTTCCCCAGA GAGAGCTGAG 
GATTCTGATG CACTGACGGC TGTCAGCAGT CAGCTAGAAG GCTCTCCTAT GGATACAAGC 
AGCCTGGCTT CCTGTACCTT AGAGGAGGCT GTGGGTGACA CTTCAGCAGC TGGCAGTTCT 
GAGCAGCCCA GAGCAGGCAG CTCCACTCCT GGGGATGCCC CACCAGCTGT GGCGGAAGTG 
CAAGGCAGGA GTGATGGGTC AGGGGAATCT GCCCAGCCAC CTGAGGACAG CTCCCCACCT 
GCATCCTCTG AGAGCTCTTC CACCAGAGAT TCTGCCGTGG CCATTTCTGG AGCAGATTCC 
CGAGGAATCC TAGAAGAGCC GTTGCCTTCA ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG 
GGTATCAGTC TCCCTGAAGG TGTGGACCCC TCTTTTCTGG CTGCCCTGCC TGATGACATC 
CGTCGGGAAG TTCTACAGAA CCAGCTAGGC ATTCGTCCAC CAACCCGGAC TGCCCCCTCC 
ACAAATAGCT CAGCGCCTGC AGTGGTGGGG AATCCTGGTG TGACTGAAGT GAGCCCTGAG 
TTTCTGGCTG CCCTGCCTCC AGCCATTCAG GAGGAAGTAC TGGCACAGCA GAGAGCTGAG 
CAGCAGCGAC GAGAACTAGC ACAGAATGCC AGCTCAGACA CCCCTATGGA CCCTGTGACC 
TTCATCCAGA CTCTGCCCTC AGACCTGCGC CGTAGTGTCC TAGAGGATAT GGAGGACAGT 
GTGTTAGCTG TGATGCCACC TGACATTGCA GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA 
GAAGCCCGGC AGCGACAGCT CATGCATGAG CGTCTGTTTG GGCACAGTAG CACCTCCGCA 
CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC 
CAGTATACTC GCCTTGCTGT GCAGAGAGGT GGCACCTTCC AGATGGGGGG TAGCAGCAGC 
CATAACAGGC CTTCTGGCAG TAATGTAGAT ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT 
CTGGACCACG AAGCCCTTTC TTGTCTCTTG GTCCTACTTT TTGTGGATGA GCCAAAGCTC 
AATACTAGCC GTCTACACCG AGTACTGAGA AATCTCTGCT ACCATGCCCA GACCCGCCAC 
TGGGTCATCC GCAGTCTGCT CTCCATCTTG CAGCGCAGCA GTGAGAGTGA GCTATGCATT 
GAAACACCCA AACTCACTAC AAGTGAGGAA AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG 
TCAAGTAGCC ATGAGAACCG TCCCCTGGAC CTGCTACACA AGATGGAGTC AAAGAGCTCC 
AACCAGCTTT CCTGGCTCTC AGTATCCATG GATGCAGCCC TAGGCTGCAG GACTAATATA 
TTTCAGATCC AGCGTTCAGG GGGGCGTAAA CATACCGAGA AGCATGCAAG CGGTGGCTCC 
ACCGTCCACA TCCATCCCCA AGCTGCTCCT GTTGTCTGCA GACACGTTTT GGATACACTC 
ATTCAATTGG CCAAGGTATT TCCCAGCCAC TTCACACAGC AGCGGACCAA AGAAACAAAC 
TGTGAGAGTG ATCGGGAAAG GGGCAATAAG GCCTGTAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TTTGCACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTC 
AGCCGGAAAG GCAAGAACTC CGTGAAGTCA GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA 
ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAC 
CCAGTCATCC GCCGGAGCTC TCTCTTAACT GAGAAACTCC TCAGACTCCT TTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAGGTGTCA GAAGCACAGG CTAATTCTGG CAGCGGTGCT 
TCCTCCACCA CCACTGCCAC CTCAACCACA TCTACCACCA CCACCACTGC CGCCTCCACC 
ACGCCCACAC CCCCTACTGC ACCCACCCCT GTCACTTCTG CTCCAGCCCT GGTTGCTGCC 
ACGGCTATTT CCACCATTGT CGTAGCTGCT TCGACCACAG TGACTACCCC CACGACTGCT 
ACCACTACTG TTTCAATTTC TCCCACTACT AAGGGCAGCA AATCTCCAGC GAAGGTGAGT 
GATGGGGGCA GCAGCAGTAC AGACTTTAAG ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAGGAAGG CTTAGAGGAT 
GCAGCCAACG TACTACTGCA GCTCTCCCGG GGGGACTCTG GGACCCGGGA CACTGTTCTC 
AAGCTGCTAC TGAATGGAGC CCGCCATCTG GGTTATACCC TTTGTAAACA AATAGGTACC 
CTGCTGGCCG AGCTGCGGGA ATACAACCTC GAGCAGCAGC GGCGAGCCCA ATGTGAAACC 
CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG CCACAGACCA CCAAGCTGAA GGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAGAATGTG GTAATTGTGG CATCTCAGAA GCGACCTTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTCC GGGACGACAC GCGCCGGGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTAG CCAGTCAGAG 
GCGTCTGTCC GGAGGGAGGA ATCACCCATG GATGTGGACC AGCCATCTCC CAGTGCTCAA 
GATACTCAAT CCATTGCCTC CGATGGAACC CCACAGGGGG AGAAGGAAAA GGAAGAAAGA 
CCACCTGAGT TACCCCTGCT CAGCGAGCAG CTGAGTTTGG ACGAGCTGTG GGACATGCTT 
GGGGAGTGTC TAAAGGAACT AGAGGAATCC CATGACCAGC ATGCGGTGCT AGTGCTACAG 
CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT GCCACAGAGC GGGAGAGCAA GCCTCCTGTC 
CGAGACACCC GTGAGAGCCA GCTGGCACAC ATCAAGGACG AGCCTCCTCC ACTCTCCCCT 
GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC CTTGACCCAT TCTTCTCCCG GGAGCCCTCA 
TCTATGCACA TCTCCTCAAG CCTGCCCCCT GACACACAGA AGTTCCTTCG CTTTGCAGAG 
ACTCACCGCA CTGTGTTAAA CCAGATCCTA CGGCAGTCCA CGACCCACCT TGCTGATGGG 
CCTTTTGCTG TCCTGGTAGA CTACATTCGT GTCCTCGACT TTGATGTCAA GCGCAAATAT 
TTCCGCCAAG AGCTGGAGCG TTTAGATGAG GGGCTCCGGA AAGAAGACAT GGCTGTGCAT 
GTCCGTCGTG ACCATGTGTT TGAAGACTCC TATCGTGAGC TGCATCGCAA ATCCCCCGAA 
GAAATGAAGA ATCGATTGTA TATAGTATTT GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG 
CTCCTGCGGG AGTGGTATAT GATCATCTCT CGAGAGATGT TTAACCCTAT GTATGCCTTG 
TTCCGTACCT CACCTGGTGA TCGAGTCACC TACACCATCA ATCCATCTTC CCACTGCAAC 
CCCAACCACC TCAGCTACTT CAAGTTTGTC GGACGCATTG TGGCCAAAGC TGTATATGAC 
AACCGTCTTC TGGAGTGCTA CTTTACTCGA TCCTTTTACA AACACATCTT GGGCAAGTCA 
GTCAGATATA CAGATATGGA GAGTGAAGAT TACCACTTCT ACCAAGGTCT GGTTTATCTG 
CTGGAAAATG ATGTCTCCAC ACTAGGCTAT GACCTCACCT TCAGCACTGA GGTCCAAGAG 
TTTGGAGTTT GTGAAGTTCG TGACCTCAAA CCCAATGGGG CCAACATCTT GGTAACAGAG 
GAGAATAAGA AGGAGTATGT ACACCTGGTA TGCCAGATGA GAATGACAGG AGCCATCCGC 
AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC TATGAGATCA TTCCAAAGCG CCTCATTTCC 
ATCTTCACTG AGCAGGAGTT AGAGCTGCTT ATATCAGGAC TGCCCACCAT TGACATCGAT 
GATCTGAAAT CCAACACTGA ATACCACAAG TACCAGTCCA ACTCTATTCA GATCCAGTGG 
TTCTGGAGAG CATTGCGTTC TTTCGATCAA GCTGACCGTG CCAAGTTCCT CCAGTTTGTC 
ACAGGTACTT CCAAGGTACC CCTGCAAGGC TTTGCTGCCC TCGAAGGCAT GAATGGCATT 
CAGAAGTTTC AGATCCATCG AGATGACAGG TCCACAGATC GCCTGCCTTC AGCTCACACA 
TGTTTTAATC AGCTGGATCT GCCTGCCTAT GAGAGCTTTG AGAAGCTCCG CCACATGCTA 
CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884682.1
CDS	3018..16502
Translation	MDPFISRREQKCMEIGTPRKNGVLVLRSSEAVYHLTTPFQIVCLHLPLSKSKPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029193.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029193.1

ORF Insert Sequence:

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTATTC CAATGCCTTG CAGGAATCAG CAAACAGTAT CTTGTATAAT 
GGCTTGATAG AGGAGTTGGT AGATGTCCTT CAGATAACGG ATAAGCAGCT TATGGAGATT 
AAAGCAGCTT CTTTACGAAC ATTAACATCA ATTGTCCACT TGGAGAGAAC TCCCAAACTC 
AGCAGTATTA TTGACTGTAC TGGAACTGCC TCCTACCATG GATTTTTGCC AGTGCTTGTA 
AGGAACTGTA TCCAGGCCAT GATTGATCCT TCCATGGATC CATACCCTCA CCAGTTTGCC 
ACTGCTCTCT TCTCTTTTTT ATACCATCTG GCCAGCTACG ATGCTGGTGG TGAAGCCTTG 
GTCTCCTGTG GAATGATGGA AGCCTTATTG AAGGTCATAA AGTTTCTTGG CGATGAACAG 
GACCAGATAA CATTTGTCAC CAGAGCCGTC AGAGTGGTTG ACCTTATCAC CAACCTGGAT 
ATGGCAGCTT TTCAATCCCA TAGTGGACTT TCTATCTTCA TTTATAGACT TGAGCATGAA 
GTAGATTTGT GCCGAAAAGA ATGTCCGTTT GTGATCAAGC CAAAGATCCA GAGACCCAAT 
ACTACACAAG AAGGAGAGGA AATGGAAACT GATATGGATG TTGCAGATGT GGCTATGGAA 
AGCAGTCCAG GCTCATCCAT CTCTATGGAG CACAGGCTGG ATGTTGAATT AAGGGCATCA 
GGTTCCAGCA GCAGCACTAA CATCTCTTCT GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC 
GGCACCGGCC CTGGCCCCGG CCCCGGCCCC GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC 
GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT 
ATTCCACAAC GAGCAGCACT TCTGAAATCC ATGTTGAATT TCCTCAAGAA GGCCATCCAA 
GACCCTGCTT TCTCAGATGG CATACGACAT GTGATGGATG GTTCTCTGCC TACCTCCCTG 
AAACACATCA TCAGCAATGC AGAATACTAT GGCCCATCAC TCTTCCTCCT AGCTACTGAA 
GTGGTGACTG TGTTTGTATT TCAAGAACCA TCACTGCTCT CCTCACTCCA GGACAATGGA 
TTGACAGATG TCATGCTGCA TGCACTGCTT ATCAAAGATG TTCCTGCTAC CCGTGAAGTC 
CTTGGCTCCC TCCCAAATGT ATTCAGTGCA CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT 
TTTGTTCAGT GTCAGCCTTT TGAACGCCTC TTCAAAGTTC TTCTGTCTCC AGATTACCTC 
CCAGCCATGC GGAGGAGGAG AAGTTCTGAT CCCCTTGGGG ATACTGCATC CAACCTGGGG 
AGTGCTGTCG ATGAGCTCAT GAGACATCAG CCCACCCTTA AAACAGATGC AACGACTGCC 
ATCATCAAGT TACTTGAAGA AATCTGTAAT CTTGGAAGGG ACCCCAAATA CATCTGTCAG 
AAGCCATCAA TCCAGAAGGC AGATGGCACT GCCACTGCTC CTCCCCCAAG GTCTAATCAT 
GCCGCAGAAG AAGCCTCTAG TGAGGATGAG GAGGAAGAGG AAGTACAGGC CATGCAGAGC 
TTTAATTCTA CCCAGCAAAA TGAAACTGAG CCTAATCAGC AGGTTGTTGG TACAGAGGAA 
CGTATTCCTA TTCCCCTCAT GGATTACATC CTTAATGTGA TGAAATTTGT GGAATCTATT 
CTGAGCAACA ATACAACAGA TGACCACTGC CAGGAATTTG TGAATCAGAA AGGACTGTTG 
CCTTTGGTTA CCATTTTGGG TCTTCCCAAT CTGCCCATTG ACTTTCCCAC ATCTGCTGCC 
TGTCAGGCTG TTGCAGGTGT CTGCAAATCC ATATTGACAC TGTCACATGA ACCCAAAGTC 
CTTCAAGAGG GTCTCCTTCA GTTGGACTCC ATCCTCTCCT CCCTGGAGCC CTTACACCGC 
CCCATTGAAT CCCCTGGGGG CTCAGTGTTG TTGCGAGAAC TGGCTTGCGC AGGCAATGTT 
GCTGATGCTA CCCTCTCAGC CCAGGCCACA CCTCTGCTGC ATGCACTCAC TGCTGCCCAT 
GCCTACATCA TGATGTTTGT TCATACTTGC AGAGTTGGAC AGAGTGAAAT TCGTTCCATC 
TCCGTAAACC AGTGGGGCTC TCAATTGGGT CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA 
TACTGTTCCC TGGTGTGGGA AAGCACTGTC CTCCTCTCTC TGTGTACCCC AAACAGCCTA 
CCATCTGGGT GTGAATTTGG CCAGGCAGAT ATGCAGAAAC TGGTTCCAAA GGATGAGAAG 
GCAGGTACGA CCCAGGGCGG AAAAAGATCA GATGGGGAAC AGGATGGAGC AGCTGGAAGT 
ATGGATGCTT CTACCCAGGG CTTATTAGAA GGCATTGGGC TAGATGGTGA CACATTGGCT 
CCCATGGAGA CAGATGAACC TACTGCTTCA GACTCTAAGG GCAAATCTAA AATCACACCA 
GCAATGGCTG CCAGAATTAA GCAAATCAAG CCTTTGTTAT CAGCTTCCTC CAGATTAGGC 
CGAGCACTTG CTGAGCTATT TGGACTTCTT GTTAAACTTT GTGTGGGATC TCCTGTCCGC 
CAGAGAAGGA GCCATCATGC TGCCAGCACC ACTACAGCAC CGACACCTGC CGCGCGATCA 
ACAGCCTCAG CTCTCACTAA GCTCTTGACT AAGGGGTTAT CTTGGCAGCC CCCACCATAT 
ACACCTACTC CCCGATTCAG GCTGACATTC TTCATCTGTT CAGTTGGTTT CACATCCCCA 
ATGCTGTTTG ATGAGAGGAA GTATCCCTAC CACCTCATGC TGCAAAAATT TCTCTGCTCC 
GGAGGCCACA ATGCTCTTTT TGAAACTTTC AACTGGGCTC TGTCCATGGG AGGTAAAGTT 
CCTGTTTCTG AGGGATTGGA ACACTCAGAC TTGCCTGATG GCACAGGAGA ATTCCTAGAT 
GCCTGGCTTA TGCTGGTGGA GAAGATGGTG AATCCCACCA CGGTGCTTGA ATCTCCACAT 
TCGCTGCCTG CCAAATTGCC TGGAGGTGTC CAGAACTTTC CCCAGTTCAG TGCACTGCGC 
TTCCTTGTGG TAACTCAGAA AGCAGCCTTT ACTTGCATCA AAAACTTATG GAACCGGAAA 
CCCCTGAAGG TATATGGTGG ACGAATGGCT GAATCGATGC TGGCCATTCT ATGCCACATC 
CTCCGAGGAG AACCTGTGAT TCGAGAGAGA CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA 
GAAGAGGATA CAGGGCAAGA GGAAGGTGGC TCCCGCCGGG AACCTCAAGT CAACCAGCAA 
CAACTGCAAC AGCTCATGGA CATGGGCTTC ACAAGGGAAC ATGCAATGGA GGCACTGTTG 
AACACCAGCA CCATGGAGCA GGCCACAGAG TACCTTTTAA CCCACCCTCC TCCAATCATG 
GGAGGAGTTG TTCGGGATCT CAGCATGTCT GAAGAGGACC AGATGATGAG AGCAATTGCT 
ATGTCTCTGG GACAGGATAT TCCAATGGAT CAAAGGGCAG AGTCACCTGA GGAAGTTGCT 
TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA 
TGTCTAGAGA AGTTCCAGGA TGCTGACCCG TTGGAACAAG ATGAGCTCCA CACTTTCACA 
GATACTATGT TGCCAGGCTG CTTCCACCTT CTTGATGAGC TGCCAGACAC AGTATACCGT 
GTGTGTGACC TGATCATGAC AGCAATCAAA CGTAATGGAG CAGATTATCG TGACATGATT 
CTGAAGCAAG TAGTCAATCA GGTGTGGGAA GCTGCTGATG TATTGATCAA AGCTGCTCTT 
CCCCTGACAA CAAGTGACAC AAAAACCGTG TCAGAGTGGA TAAGTCAGAT GGCCACACTG 
CCCCAGGCCT CCAATTTGGC TACTAGAATC TTGCTTTTAA CGCTACTTTT TGAGGAGTTG 
AAGCTACCTT GTGCTTGGGT GGTTGAATCA AGTGGCATCC TTAATGTCCT AATCAAACTC 
TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA GCCAAGGAGC AGAAGGAAGT CCAGACCCCA 
AAGTGGATCA CACCAGTGTT GCTCCTGATT GATTTCTATG AAAAGACAGC CATCTCCTCA 
AAAAGGAGAG CCCAGATGAC TAAGTACCTG CAATCCAACA GCAACAACTG GCGCTGGTTT 
GATGATCGCT CTGGGCGTTG GTGTAGTTAC AGTGCAAGCA ACAATAGCAC TATTGATTCT 
GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA TTCACTGCAG GCCGAAGAAG ATACACGGTC 
CAATTCACTA CAATGGTGCA GGTTAATGAG GAAACAGGGA ACCGACGCCC TGTGATGCTG 
ACTCTCCTCA GGGTACCTCG GCTGAATAAA AATTCAAAAA ACAGCAATGG ACAGGAACTA 
GAGAAGACGC TGGAAGAAAG CAAAGAAATG GATATCAAAC GTAAAGAAAA TAAAGGCAAT 
GATACCCCTT TGGCCCTAGA GAGTACAAAC ACTGAAAAGG AGACAAGCCT GGAGGAAACA 
AAAATCGGGG AGATCCTGAT CCAGGGCTTG ACAGAAGATA TGGTGACTGT TTTAATCCGG 
GCCTGCGTGA GCATGCTGGG AGTCCCTGTG GACCCAGATA CTTTGCATGC CACCCTTCGT 
CTCTGTCTGA GGCTCACCCG GGACCACAAA TATGCCATGA TGTTTGCAGA ACTGAAGAGT 
ACCCGCATGA TCTTGAATTT GACCCAGAGC TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC 
ACCCTTCTCT TAAGACACAT CATTGAGGAC CCCTGTACCC TTCGTCATAC CATGGAAAAG 
GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT GGTAGCACTA CCTCTGGTGT TGTGTCTGGC 
AGCCTCGGCT CTCGGGAGAT CAACTACATC CTTCGTGTCC TTGGGCCAGC CGCATGCCGC 
AATCCAGACA TATTCACAGA AGTGGCCAAC TGCTGTATCC GCATCGCCCT TCCTGCCCCT 
CGAGGCTCAG GAACTGCTTC AGATGATGAA TTTGAGAATC TTAGAATTAA AGGCCCTAAT 
GCTGTACAGC TGGTGAAGAC CACCCCTTTG AAGCCCTCAC CTCTGCCTGT CATCCCTGAT 
ACTATCAAGG AAGTGATCTA TGATATGCTG AATGCTCTGG CTGCATACCA TGCTCCAGAG 
GAAGCAGATA AATCTGATCC TAAACCTGGG GTTATGACCC AAGAGGTTGG CCAGCTCCTG 
CAAGACATGG GTGATGATGT ATACCAGCAG TACCGGTCAC TTACGCGTCA GAGCAGTGAC 
TTTGATACGC AGTCAGGTTT TTCCATTAAT AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC 
ACTGAGACTT CCGCATCTGG GACCTCCCAA GGAGAGGCTT CAACTCCAGA GGAGTCTCGA 
GATGGGAAGA AAGATAAAGA AGGGGACCGG GCCTCTGAGG AAGGCAAACA GAAAGGCAAG 
GGCAGCAAAC CTTTAATGCC TACCTCCACT ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG 
TCCTATGTTG GTATTGCTAC CCTGATTGCC AACTACAGCT ACACTGTGGG CCAGTCTGAA 
CTGATCAAAG AGGACTGCAG TGTGCTAGCT TTTGTTCTGG ACCACCTGCT CCCACATACC 
CAGAATGCAG AAGACAAGGA CACCCCTGCC TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT 
GCTGCAGGGA GTGGCACAGA TGCCCAGGTG GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT 
GGACGGGCAC TGGCTATGGC TGAGAGTACA GAGAAACATG CCAGGCTTCA GGCAGTGATG 
TGTATCATCA GTACTATCAT GGAGTCCTGC CCCTCCACCT CCAGCTTCTA CAGCAGTGCC 
ACAGCGAAGA CCCAGCACAA TGGCATGAAC AACATCATTC GGCTTTTCCT GAAGAAGGGA 
CTGGTTAATG ACCTGGCCAG AGTACCTCAC AGCTTAGACC TGTCCAGTCC CAACATGGCC 
AACACAGTCA ATGCTGCTCT GAAGCCTTTG GAAACACTTT CCCGGATTGT GAACCAGCCC 
AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT AGCAAGAACA AGTCTGAGCA GGATGCCCAA 
GGAGCCTCTC AAGATTCCAG TAGCAACCAG CAGGACCCAG GCGAGCCTGG GGAAGCAGAA 
GTGCAGGAGG AGGATCATGA TGTCACTCAG ACAGAGGTGG CAGATGGGGA TATCATGGAT 
GGGGAGGCTG AAACCGACTC AGTGGTGATT GCTGGGCAGC CTGAGGTGCT CAGTTCACAA 
GAGATGCAGG TTGAGAATGA GCTGGAGGAC CTGATAGATG AGTTGCTTGA GAGGGATGGC 
GGATCTGGGA ACAGTACAAT TATAGTGAGC AGAAGTGGAG AGGATGAATC ACAAGAGGAC 
GTGCTGATGG ATGAAGCTCC TTCCAACCTC AGCCAAGCTT CCACCTTGCA GGCCAACCGA 
GAAGATTCCA TGAATATCCT GGACCCTGAG GATGAGGAGG AGCACACTCA GGAAGAGGAC 
AGCAGTGGCA GTAACGAGGA TGAGGATGAT AGTCAGGATG AAGAGGAGGA GGAGGAGGAA 
GATGAGGAAG ATGATCAGGA GGATGATGAA GGTGAAGAGG GAGATGAAGA CGATGACGAC 
GATGGCTCTG AGATGGAATT GGATGAGGAT TATCCTGATA TGAACGCTTC TCCCTTGGTC 
CGATTTGAGC GCTTTGACCG GGAGGATGAT CTCATCATTG AGTTTGACAA CATGTTCTCC 
AGTGCTACAG ACATCCCCCC ATCCCCAGGA AATATCCCTA CCACCCATCC ACTGATGGTG 
CGCCATGCAG ACCACAGTTC TCTGACACTG GGCAGTGGCT CTTCAACAAC TCGTCTCACC 
CAGGGCATCG GGCGCAGTCA GAGGACCCTA AGGCAGCTGA CGGCCAATAC TGGCCACACC 
ATTCATGTTC ACTACCCTGG GAATCGCCAG CCCAACCCTC CTCTTATACT GCAGAGGTTG 
CTTGGTCCCT CAGCTGCTGC TGACATCCTT CAGCTGAGCA GCAGCCTTCC CCTACAAAGC 
CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC GATGACGTCC ACATCATCGC CCGTTCTGAT 
GATGAGCTGC TGGATGACTT TTTCCATGAT CAGAGCACAG CTACCAGCCA AGCAGGAACC 
CTGTCCAGCA TCCCCACAGC CCTGACCCGC TGGACAGAAG AATGCAAAGT TCTCGATGCT 
GAGAGCATGC ATGACTGTGT TTCAGTGGTT AAAGTGTCCA TTGTCAATCA CCTGGAATTC 
CTGAGGGATG AGGAGCTGGA AGAAAGGCGA GAGAAGCGCA GGAAACAACT GGCTGAGGAA 
GAAACAAAGA TAACTGATAA AGGCAAAGAA GATAAGGAGA ACAGGGATCA GAGTGCACAG 
TGTACTGCAT CTAAGTCAAA TGACTCCACT GAACAGAATC TCTCAGATGG GACGCCTATG 
CCTGACAGCT ACCCAACAAC CCCATCTTCA ACTGATGCAG CTACATCTGA GTCCAAGGAG 
ACCCTTGGCA CTCTGCAATC CTCACAACAG CAACCAACAC TCCCAACCCC ACCAGCTTTG 
GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA GCTGGAGAAG GGGGCAGCTC TACACAGCTA 
TTGATGCCTG TAGAGCCAGA GGAATTGGGT CCCACAAGGC CAAGTGGGGA AGCAGAAACA 
ACTCAGATGG AGTTATCCCC AGCTCCCACT ATAACCTCAC TTTCCCCAGA GAGAGCTGAG 
GATTCTGATG CACTGACGGC TGTCAGCAGT CAGCTAGAAG GCTCTCCTAT GGATACAAGC 
AGCCTGGCTT CCTGTACCTT AGAGGAGGCT GTGGGTGACA CTTCAGCAGC TGGCAGTTCT 
GAGCAGCCCA GAGCAGGCAG CTCCACTCCT GGGGATGCCC CACCAGCTGT GGCGGAAGTG 
CAAGGCAGGA GTGATGGGTC AGGGGAATCT GCCCAGCCAC CTGAGGACAG CTCCCCACCT 
GCATCCTCTG AGAGCTCTTC CACCAGAGAT TCTGCCGTGG CCATTTCTGG AGCAGATTCC 
CGAGGAATCC TAGAAGAGCC GTTGCCTTCA ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG 
GGTATCAGTC TCCCTGAAGG TGTGGACCCC TCTTTTCTGG CTGCCCTGCC TGATGACATC 
CGTCGGGAAG TTCTACAGAA CCAGCTAGGC ATTCGTCCAC CAACCCGGAC TGCCCCCTCC 
ACAAATAGCT CAGCGCCTGC AGTGGTGGGG AATCCTGGTG TGACTGAAGT GAGCCCTGAG 
TTTCTGGCTG CCCTGCCTCC AGCCATTCAG GAGGAAGTAC TGGCACAGCA GAGAGCTGAG 
CAGCAGCGAC GAGAACTAGC ACAGAATGCC AGCTCAGACA CCCCTATGGA CCCTGTGACC 
TTCATCCAGA CTCTGCCCTC AGACCTGCGC CGTAGTGTCC TAGAGGATAT GGAGGACAGT 
GTGTTAGCTG TGATGCCACC TGACATTGCA GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA 
GAAGCCCGGC AGCGACAGCT CATGCATGAG CGTCTGTTTG GGCACAGTAG CACCTCCGCA 
CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC 
CAGTATACTC GCCTTGCTGT GCAGAGAGGT GGCACCTTCC AGATGGGGGG TAGCAGCAGC 
CATAACAGGC CTTCTGGCAG TAATGTAGAT ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT 
CTGGACCACG AAGCCCTTTC TTGTCTCTTG GTCCTACTTT TTGTGGATGA GCCAAAGCTC 
AATACTAGCC GTCTACACCG AGTACTGAGA AATCTCTGCT ACCATGCCCA GACCCGCCAC 
TGGGTCATCC GCAGTCTGCT CTCCATCTTG CAGCGCAGCA GTGAGAGTGA GCTATGCATT 
GAAACACCCA AACTCACTAC AAGTGAGGAA AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG 
TCAAGTAGCC ATGAGAACCG TCCCCTGGAC CTGCTACACA AGATGGAGTC AAAGAGCTCC 
AACCAGCTTT CCTGGCTCTC AGTATCCATG GATGCAGCCC TAGGCTGCAG GACTAATATA 
TTTCAGATCC AGCGTTCAGG GGGGCGTAAA CATACCGAGA AGCATGCAAG CGGTGGCTCC 
ACCGTCCACA TCCATCCCCA AGCTGCTCCT GTTGTCTGCA GACACGTTTT GGATACACTC 
ATTCAATTGG CCAAGGTATT TCCCAGCCAC TTCACACAGC AGCGGACCAA AGAAACAAAC 
TGTGAGAGTG ATCGGGAAAG GGGCAATAAG GCCTGTAGCC CATGCTCCTC ACAGTCCTCC 
AGCAGTGGCA TTTGCACAGA CTTCTGGGAC TTATTGGTAA AACTGGACAA CATGAATGTC 
AGCCGGAAAG GCAAGAACTC CGTGAAGTCA GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA 
ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA CTGGGGCAGC TCATGAACAT GTTGTCACAC 
CCAGTCATCC GCCGGAGCTC TCTCTTAACT GAGAAACTCC TCAGACTCCT TTCTCTCATC 
TCAATTGCTC TCCCAGAAAA CAAGGTGTCA GAAGCACAGG CTAATTCTGG CAGCGGTGCT 
TCCTCCACCA CCACTGCCAC CTCAACCACA TCTACCACCA CCACCACTGC CGCCTCCACC 
ACGCCCACAC CCCCTACTGC ACCCACCCCT GTCACTTCTG CTCCAGCCCT GGTTGCTGCC 
ACGGCTATTT CCACCATTGT CGTAGCTGCT TCGACCACAG TGACTACCCC CACGACTGCT 
ACCACTACTG TTTCAATTTC TCCCACTACT AAGGGCAGCA AATCTCCAGC GAAGGTGAGT 
GATGGGGGCA GCAGCAGTAC AGACTTTAAG ATGGTGTCCT CTGGCCTCAC TGAAAACCAG 
CTACAGCTCT CTGTAGAGGT GTTGACATCC CACTCTTGTT CTGAGGAAGG CTTAGAGGAT 
GCAGCCAACG TACTACTGCA GCTCTCCCGG GGGGACTCTG GGACCCGGGA CACTGTTCTC 
AAGCTGCTAC TGAATGGAGC CCGCCATCTG GGTTATACCC TTTGTAAACA AATAGGTACC 
CTGCTGGCCG AGCTGCGGGA ATACAACCTC GAGCAGCAGC GGCGAGCCCA ATGTGAAACC 
CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG CCACAGACCA CCAAGCTGAA GGGCAAAATG 
CAGAGCAGGT TTGACATGGC TGAGAATGTG GTAATTGTGG CATCTCAGAA GCGACCTTTG 
GGTGGCCGGG AGCTCCAGCT GCCTTCTATG TCCATGTTGA CATCCAAGAC ATCTACCCAG 
AAGTTCTTCT TGAGGGTACT ACAGGTCATC ATCCAGCTCC GGGACGACAC GCGCCGGGCT 
AACAAGAAAG CCAAGCAGAC AGGCAGGCTA GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC 
ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT GAGGCTGATG CCATTATACA AATGGTACGT 
GAGGGTCAAA GGGCGCGGAG ACAGCAACAA GCAGCAACGT CGGAGTCTAG CCAGTCAGAG 
GCGTCTGTCC GGAGGGAGGA ATCACCCATG GATGTGGACC AGCCATCTCC CAGTGCTCAA 
GATACTCAAT CCATTGCCTC CGATGGAACC CCACAGGGGG AGAAGGAAAA GGAAGAAAGA 
CCACCTGAGT TACCCCTGCT CAGCGAGCAG CTGAGTTTGG ACGAGCTGTG GGACATGCTT 
GGGGAGTGTC TAAAGGAACT AGAGGAATCC CATGACCAGC ATGCGGTGCT AGTGCTACAG 
CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT GCCACAGAGC GGGAGAGCAA GCCTCCTGTC 
CGAGACACCC GTGAGAGCCA GCTGGCACAC ATCAAGGACG AGCCTCCTCC ACTCTCCCCT 
GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC CTTGACCCAT TCTTCTCCCG GGAGCCCTCA 
TCTATGCACA TCTCCTCAAG CCTGCCCCCT GACACACAGA AGTTCCTTCG CTTTGCAGAG 
ACTCACCGCA CTGTGTTAAA CCAGATCCTA CGGCAGTCCA CGACCCACCT TGCTGATGGG 
CCTTTTGCTG TCCTGGTAGA CTACATTCGT GTCCTCGACT TTGATGTCAA GCGCAAATAT 
TTCCGCCAAG AGCTGGAGCG TTTAGATGAG GGGCTCCGGA AAGAAGACAT GGCTGTGCAT 
GTCCGTCGTG ACCATGTGTT TGAAGACTCC TATCGTGAGC TGCATCGCAA ATCCCCCGAA 
GAAATGAAGA ATCGATTGTA TATAGTATTT GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG 
CTCCTGCGGG AGTGGTATAT GATCATCTCT CGAGAGATGT TTAACCCTAT GTATGCCTTG 
TTCCGTACCT CACCTGGTGA TCGAGTCACC TACACCATCA ATCCATCTTC CCACTGCAAC 
CCCAACCACC TCAGCTACTT CAAGTTTGTC GGACGCATTG TGGCCAAAGC TGTATATGAC 
AACCGTCTTC TGGAGTGCTA CTTTACTCGA TCCTTTTACA AACACATCTT GGGCAAGTCA 
GTCAGATATA CAGATATGGA GAGTGAAGAT TACCACTTCT ACCAAGGTCT GGTTTATCTG 
CTGGAAAATG ATGTCTCCAC ACTAGGCTAT GACCTCACCT TCAGCACTGA GGTCCAAGAG 
TTTGGAGTTT GTGAAGTTCG TGACCTCAAA CCCAATGGGG CCAACATCTT GGTAACAGAG 
GAGAATAAGA AGGAGTATGT ACACCTGGTA TGCCAGATGA GAATGACAGG AGCCATCCGC 
AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC TATGAGATCA TTCCAAAGCG CCTCATTTCC 
ATCTTCACTG AGCAGGAGTT AGAGCTGCTT ATATCAGGAC TGCCCACCAT TGACATCGAT 
GATCTGAAAT CCAACACTGA ATACCACAAG TACCAGTCCA ACTCTATTCA GATCCAGTGG 
TTCTGGAGAG CATTGCGTTC TTTCGATCAA GCTGACCGTG CCAAGTTCCT CCAGTTTGTC 
ACAGGTACTT CCAAGGTACC CCTGCAAGGC TTTGCTGCCC TCGAAGGCAT GAATGGCATT 
CAGAAGTTTC AGATCCATCG AGATGACAGG TCCACAGATC GCCTGCCTTC AGCTCACACA 
TGTTTTAATC AGCTGGATCT GCCTGCCTAT GAGAGCTTTG AGAAGCTCCG CCACATGCTA 
CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029194.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884683.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X4, mRNA.

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CloneID	OHu99115
Clone ID Related Accession (Same CDS sequence)	XM_017029194.1
Accession Version	XM_017029194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GTTGCAGATG TGGCTATGGA AAGCAGTCCA GGCTCATCCA TCTCTATGGA GCACAGGCTG 
GATGTTGAAT TAAGGGCATC AGGTTCCAGC AGCAGCACTA ACATCTCTTC TGGCCCCAGC 
CCTGGTCCCA GTCCCGGCCC CGGCACCGGC CCTGGCCCCG GCCCCGGCCC CGGCCCCGGC 
CCTGGCCCCG GCCCCGGCCC CGGTCCTGGT CCCGGCCCTG GCCCCGGCCC TGGCCCTGGC 
CCCCGTCCTG GAGTCCAGTG TATTCCACAA CGAGCAGCAC TTCTGAAATC CATGTTGAAT 
TTCCTCAAGA AGGCCATCCA AGACCCTGCT TTCTCAGATG GCATACGACA TGTGATGGAT 
GGTTCTCTGC CTACCTCCCT GAAACACATC ATCAGCAATG CAGAATACTA TGGCCCATCA 
CTCTTCCTCC TAGCTACTGA AGTGGTGACT GTGTTTGTAT TTCAAGAACC ATCACTGCTC 
TCCTCACTCC AGGACAATGG ATTGACAGAT GTCATGCTGC ATGCACTGCT TATCAAAGAT 
GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC CTCCCAAATG TATTCAGTGC ACTCTGTTTG 
AATGCCCGAG GTCTTCAGTC TTTTGTTCAG TGTCAGCCTT TTGAACGCCT CTTCAAAGTT 
CTTCTGTCTC CAGATTACCT CCCAGCCATG CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG 
GATACTGCAT CCAACCTGGG GAGTGCTGTC GATGAGCTCA TGAGACATCA GCCCACCCTT 
AAAACAGATG CAACGACTGC CATCATCAAG TTACTTGAAG AAATCTGTAA TCTTGGAAGG 
GACCCCAAAT ACATCTGTCA GAAGCCATCA ATCCAGAAGG CAGATGGCAC TGCCACTGCT 
CCTCCCCCAA GGTCTAATCA TGCCGCAGAA GAAGCCTCTA GTGAGGATGA GGAGGAAGAG 
GAAGTACAGG CCATGCAGAG CTTTAATTCT ACCCAGCAAA ATGAAACTGA GCCTAATCAG 
CAGGTTGTTG GTACAGAGGA ACGTATTCCT ATTCCCCTCA TGGATTACAT CCTTAATGTG 
ATGAAATTTG TGGAATCTAT TCTGAGCAAC AATACAACAG ATGACCACTG CCAGGAATTT 
GTGAATCAGA AAGGACTGTT GCCTTTGGTT ACCATTTTGG GTCTTCCCAA TCTGCCCATT 
GACTTTCCCA CATCTGCTGC CTGTCAGGCT GTTGCAGGTG TCTGCAAATC CATATTGACA 
CTGTCACATG AACCCAAAGT CCTTCAAGAG GGTCTCCTTC AGTTGGACTC CATCCTCTCC 
TCCCTGGAGC CCTTACACCG CCCCATTGAA TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA 
CTGGCTTGCG CAGGCAATGT TGCTGATGCT ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG 
CATGCACTCA CTGCTGCCCA TGCCTACATC ATGATGTTTG TTCATACTTG CAGAGTTGGA 
CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC CAGTGGGGCT CTCAATTGGG TCTGAGTGTT 
TTGAGCAAGC TGAGCCAGTT ATACTGTTCC CTGGTGTGGG AAAGCACTGT CCTCCTCTCT 
CTGTGTACCC CAAACAGCCT ACCATCTGGG TGTGAATTTG GCCAGGCAGA TATGCAGAAA 
CTGGTTCCAA AGGATGAGAA GGCAGGTACG ACCCAGGGCG GAAAAAGATC AGATGGGGAA 
CAGGATGGAG CAGCTGGAAG TATGGATGCT TCTACCCAGG GCTTATTAGA AGGCATTGGG 
CTAGATGGTG ACACATTGGC TCCCATGGAG ACAGATGAAC CTACTGCTTC AGACTCTAAG 
GGCAAATCTA AAATCACACC AGCAATGGCT GCCAGAATTA AGCAAATCAA GCCTTTGTTA 
TCAGCTTCCT CCAGATTAGG CCGAGCACTT GCTGAGCTAT TTGGACTTCT TGTTAAACTT 
TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG AGCCATCATG CTGCCAGCAC CACTACAGCA 
CCGACACCTG CCGCGCGATC AACAGCCTCA GCTCTCACTA AGCTCTTGAC TAAGGGGTTA 
TCTTGGCAGC CCCCACCATA TACACCTACT CCCCGATTCA GGCTGACATT CTTCATCTGT 
TCAGTTGGTT TCACATCCCC AATGCTGTTT GATGAGAGGA AGTATCCCTA CCACCTCATG 
CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC AATGCTCTTT TTGAAACTTT CAACTGGGCT 
CTGTCCATGG GAGGTAAAGT TCCTGTTTCT GAGGGATTGG AACACTCAGA CTTGCCTGAT 
GGCACAGGAG AATTCCTAGA TGCCTGGCTT ATGCTGGTGG AGAAGATGGT GAATCCCACC 
ACGGTGCTTG AATCTCCACA TTCGCTGCCT GCCAAATTGC CTGGAGGTGT CCAGAACTTT 
CCCCAGTTCA GTGCACTGCG CTTCCTTGTG GTAACTCAGA AAGCAGCCTT TACTTGCATC 
AAAAACTTAT GGAACCGGAA ACCCCTGAAG GTATATGGTG GACGAATGGC TGAATCGATG 
CTGGCCATTC TATGCCACAT CCTCCGAGGA GAACCTGTGA TTCGAGAGAG ACTAAGCAAG 
GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG 
GAACCTCAAG TCAACCAGCA ACAACTGCAA CAGCTCATGG ACATGGGCTT CACAAGGGAA 
CATGCAATGG AGGCACTGTT GAACACCAGC ACCATGGAGC AGGCCACAGA GTACCTTTTA 
ACCCACCCTC CTCCAATCAT GGGAGGAGTT GTTCGGGATC TCAGCATGTC TGAAGAGGAC 
CAGATGATGA GAGCAATTGC TATGTCTCTG GGACAGGATA TTCCAATGGA TCAAAGGGCA 
GAGTCACCTG AGGAAGTTGC TTGCCGGAAG GAGGAAGAGG AACGGAAAGC TCGGGAAAAG 
CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG AAGTTCCAGG ATGCTGACCC GTTGGAACAA 
GATGAGCTCC ACACTTTCAC AGATACTATG TTGCCAGGCT GCTTCCACCT TCTTGATGAG 
CTGCCAGACA CAGTATACCG TGTGTGTGAC CTGATCATGA CAGCAATCAA ACGTAATGGA 
GCAGATTATC GTGACATGAT TCTGAAGCAA GTAGTCAATC AGGTGTGGGA AGCTGCTGAT 
GTATTGATCA AAGCTGCTCT TCCCCTGACA ACAAGTGACA CAAAAACCGT GTCAGAGTGG 
ATAAGTCAGA TGGCCACACT GCCCCAGGCC TCCAATTTGG CTACTAGAAT CTTGCTTTTA 
ACGCTACTTT TTGAGGAGTT GAAGCTACCT TGTGCTTGGG TGGTTGAATC AAGTGGCATC 
CTTAATGTCC TAATCAAACT CTTGGAAGTG GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG 
CAGAAGGAAG TCCAGACCCC AAAGTGGATC ACACCAGTGT TGCTCCTGAT TGATTTCTAT 
GAAAAGACAG CCATCTCCTC AAAAAGGAGA GCCCAGATGA CTAAGTACCT GCAATCCAAC 
AGCAACAACT GGCGCTGGTT TGATGATCGC TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC 
AACAATAGCA CTATTGATTC TGCCTGGAAA TCTGGAGAGA CAAGCGTGCG ATTCACTGCA 
GGCCGAAGAA GATACACGGT CCAATTCACT ACAATGGTGC AGGTTAATGA GGAAACAGGG 
AACCGACGCC CTGTGATGCT GACTCTCCTC AGGGTACCTC GGCTGAATAA AAATTCAAAA 
AACAGCAATG GACAGGAACT AGAGAAGACG CTGGAAGAAA GCAAAGAAAT GGATATCAAA 
CGTAAAGAAA ATAAAGGCAA TGATACCCCT TTGGCCCTAG AGAGTACAAA CACTGAAAAG 
GAGACAAGCC TGGAGGAAAC AAAAATCGGG GAGATCCTGA TCCAGGGCTT GACAGAAGAT 
ATGGTGACTG TTTTAATCCG GGCCTGCGTG AGCATGCTGG GAGTCCCTGT GGACCCAGAT 
ACTTTGCATG CCACCCTTCG TCTCTGTCTG AGGCTCACCC GGGACCACAA ATATGCCATG 
ATGTTTGCAG AACTGAAGAG TACCCGCATG ATCTTGAATT TGACCCAGAG CTCAGGCTTC 
AATGGGTTTA CTCCCCTGGT CACCCTTCTC TTAAGACACA TCATTGAGGA CCCCTGTACC 
CTTCGTCATA CCATGGAAAA GGTTGTTCGC TCAGCAGCTA CAAGTGGAGC TGGTAGCACT 
ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC TCTCGGGAGA TCAACTACAT CCTTCGTGTC 
CTTGGGCCAG CCGCATGCCG CAATCCAGAC ATATTCACAG AAGTGGCCAA CTGCTGTATC 
CGCATCGCCC TTCCTGCCCC TCGAGGCTCA GGAACTGCTT CAGATGATGA ATTTGAGAAT 
CTTAGAATTA AAGGCCCTAA TGCTGTACAG CTGGTGAAGA CCACCCCTTT GAAGCCCTCA 
CCTCTGCCTG TCATCCCTGA TACTATCAAG GAAGTGATCT ATGATATGCT GAATGCTCTG 
GCTGCATACC ATGCTCCAGA GGAAGCAGAT AAATCTGATC CTAAACCTGG GGTTATGACC 
CAAGAGGTTG GCCAGCTCCT GCAAGACATG GGTGATGATG TATACCAGCA GTACCGGTCA 
CTTACGCGTC AGAGCAGTGA CTTTGATACG CAGTCAGGTT TTTCCATTAA TAGTCAGGTC 
TTTGCTGCAG ATGGTGCCTC CACTGAGACT TCCGCATCTG GGACCTCCCA AGGAGAGGCT 
TCAACTCCAG AGGAGTCTCG AGATGGGAAG AAAGATAAAG AAGGGGACCG GGCCTCTGAG 
GAAGGCAAAC AGAAAGGCAA GGGCAGCAAA CCTTTAATGC CTACCTCCAC TATCCTTCGT 
CTTCTGGCAG AGTTGGTGAG GTCCTATGTT GGTATTGCTA CCCTGATTGC CAACTACAGC 
TACACTGTGG GCCAGTCTGA ACTGATCAAA GAGGACTGCA GTGTGCTAGC TTTTGTTCTG 
GACCACCTGC TCCCACATAC CCAGAATGCA GAAGACAAGG ACACCCCTGC CTTGGCCCGC 
CTGTTCCTCG CAAGCCTGGC TGCTGCAGGG AGTGGCACAG ATGCCCAGGT GGCCCTAGTG 
AATGAAGTAA AAGCAGCCCT TGGACGGGCA CTGGCTATGG CTGAGAGTAC AGAGAAACAT 
GCCAGGCTTC AGGCAGTGAT GTGTATCATC AGTACTATCA TGGAGTCCTG CCCCTCCACC 
TCCAGCTTCT ACAGCAGTGC CACAGCGAAG ACCCAGCACA ATGGCATGAA CAACATCATT 
CGGCTTTTCC TGAAGAAGGG ACTGGTTAAT GACCTGGCCA GAGTACCTCA CAGCTTAGAC 
CTGTCCAGTC CCAACATGGC CAACACAGTC AATGCTGCTC TGAAGCCTTT GGAAACACTT 
TCCCGGATTG TGAACCAGCC CAGTAGCCTT TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC 
AAGTCTGAGC AGGATGCCCA AGGAGCCTCT CAAGATTCCA GTAGCAACCA GCAGGACCCA 
GGCGAGCCTG GGGAAGCAGA AGTGCAGGAG GAGGATCATG ATGTCACTCA GACAGAGGTG 
GCAGATGGGG ATATCATGGA TGGGGAGGCT GAAACCGACT CAGTGGTGAT TGCTGGGCAG 
CCTGAGGTGC TCAGTTCACA AGAGATGCAG GTTGAGAATG AGCTGGAGGA CCTGATAGAT 
GAGTTGCTTG AGAGGGATGG CGGATCTGGG AACAGTACAA TTATAGTGAG CAGAAGTGGA 
GAGGATGAAT CACAAGAGGA CGTGCTGATG GATGAAGCTC CTTCCAACCT CAGCCAAGCT 
TCCACCTTGC AGGCCAACCG AGAAGATTCC ATGAATATCC TGGACCCTGA GGATGAGGAG 
GAGCACACTC AGGAAGAGGA CAGCAGTGGC AGTAACGAGG ATGAGGATGA TAGTCAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGAGGAA GATGATCAGG AGGATGATGA AGGTGAAGAG 
GGAGATGAAG ACGATGACGA CGATGGCTCT GAGATGGAAT TGGATGAGGA TTATCCTGAT 
ATGAACGCTT CTCCCTTGGT CCGATTTGAG CGCTTTGACC GGGAGGATGA TCTCATCATT 
GAGTTTGACA ACATGTTCTC CAGTGCTACA GACATCCCCC CATCCCCAGG AAATATCCCT 
ACCACCCATC CACTGATGGT GCGCCATGCA GACCACAGTT CTCTGACACT GGGCAGTGGC 
TCTTCAACAA CTCGTCTCAC CCAGGGCATC GGGCGCAGTC AGAGGACCCT AAGGCAGCTG 
ACGGCCAATA CTGGCCACAC CATTCATGTT CACTACCCTG GGAATCGCCA GCCCAACCCT 
CCTCTTATAC TGCAGAGGTT GCTTGGTCCC TCAGCTGCTG CTGACATCCT TCAGCTGAGC 
AGCAGCCTTC CCCTACAAAG CCGGGGTCGG GCCCGCCTCC TGGTAGGCAA CGATGACGTC 
CACATCATCG CCCGTTCTGA TGATGAGCTG CTGGATGACT TTTTCCATGA TCAGAGCACA 
GCTACCAGCC AAGCAGGAAC CCTGTCCAGC ATCCCCACAG CCCTGACCCG CTGGACAGAA 
GAATGCAAAG TTCTCGATGC TGAGAGCATG CATGACTGTG TTTCAGTGGT TAAAGTGTCC 
ATTGTCAATC ACCTGGAATT CCTGAGGGAT GAGGAGCTGG AAGAAAGGCG AGAGAAGCGC 
AGGAAACAAC TGGCTGAGGA AGAAACAAAG ATAACTGATA AAGGCAAAGA AGATAAGGAG 
AACAGGGATC AGAGTGCACA GTGTACTGCA TCTAAGTCAA ATGACTCCAC TGAACAGAAT 
CTCTCAGATG GGACGCCTAT GCCTGACAGC TACCCAACAA CCCCATCTTC AACTGATGCA 
GCTACATCTG AGTCCAAGGA GACCCTTGGC ACTCTGCAAT CCTCACAACA GCAACCAACA 
CTCCCAACCC CACCAGCTTT GGGAGAGGTT CCTCAGGAGC TGCAGTCTCC AGCTGGAGAA 
GGGGGCAGCT CTACACAGCT ATTGATGCCT GTAGAGCCAG AGGAATTGGG TCCCACAAGG 
CCAAGTGGGG AAGCAGAAAC AACTCAGATG GAGTTATCCC CAGCTCCCAC TATAACCTCA 
CTTTCCCCAG AGAGAGCTGA GGATTCTGAT GCACTGACGG CTGTCAGCAG TCAGCTAGAA 
GGCTCTCCTA TGGATACAAG CAGCCTGGCT TCCTGTACCT TAGAGGAGGC TGTGGGTGAC 
ACTTCAGCAG CTGGCAGTTC TGAGCAGCCC AGAGCAGGCA GCTCCACTCC TGGGGATGCC 
CCACCAGCTG TGGCGGAAGT GCAAGGCAGG AGTGATGGGT CAGGGGAATC TGCCCAGCCA 
CCTGAGGACA GCTCCCCACC TGCATCCTCT GAGAGCTCTT CCACCAGAGA TTCTGCCGTG 
GCCATTTCTG GAGCAGATTC CCGAGGAATC CTAGAAGAGC CGTTGCCTTC AACAAGCAGT 
GAAGAAGAAG ATCCCCTTGC GGGTATCAGT CTCCCTGAAG GTGTGGACCC CTCTTTTCTG 
GCTGCCCTGC CTGATGACAT CCGTCGGGAA GTTCTACAGA ACCAGCTAGG CATTCGTCCA 
CCAACCCGGA CTGCCCCCTC CACAAATAGC TCAGCGCCTG CAGTGGTGGG GAATCCTGGT 
GTGACTGAAG TGAGCCCTGA GTTTCTGGCT GCCCTGCCTC CAGCCATTCA GGAGGAAGTA 
CTGGCACAGC AGAGAGCTGA GCAGCAGCGA CGAGAACTAG CACAGAATGC CAGCTCAGAC 
ACCCCTATGG ACCCTGTGAC CTTCATCCAG ACTCTGCCCT CAGACCTGCG CCGTAGTGTC 
CTAGAGGATA TGGAGGACAG TGTGTTAGCT GTGATGCCAC CTGACATTGC AGCTGAGGCT 
CAAGCCCTGA GACGAGAGCA AGAAGCCCGG CAGCGACAGC TCATGCATGA GCGTCTGTTT 
GGGCACAGTA GCACCTCCGC ACTCTCTGCT ATTCTCCGAA GCCCGGCTTT CACCAGTCGC 
TTAAGTGGCA ACCGTGGGGT CCAGTATACT CGCCTTGCTG TGCAGAGAGG TGGCACCTTC 
CAGATGGGGG GTAGCAGCAG CCATAACAGG CCTTCTGGCA GTAATGTAGA TACTCTCCTC 
CGCCTCCGAG GACGGCTCCT TCTGGACCAC GAAGCCCTTT CTTGTCTCTT GGTCCTACTT 
TTTGTGGATG AGCCAAAGCT CAATACTAGC CGTCTACACC GAGTACTGAG AAATCTCTGC 
TACCATGCCC AGACCCGCCA CTGGGTCATC CGCAGTCTGC TCTCCATCTT GCAGCGCAGC 
AGTGAGAGTG AGCTATGCAT TGAAACACCC AAACTCACTA CAAGTGAGGA AAAGGGCAAA 
AAGTCGAGCA AGAGCTGTGG GTCAAGTAGC CATGAGAACC GTCCCCTGGA CCTGCTACAC 
AAGATGGAGT CAAAGAGCTC CAACCAGCTT TCCTGGCTCT CAGTATCCAT GGATGCAGCC 
CTAGGCTGCA GGACTAATAT ATTTCAGATC CAGCGTTCAG GGGGGCGTAA ACATACCGAG 
AAGCATGCAA GCGGTGGCTC CACCGTCCAC ATCCATCCCC AAGCTGCTCC TGTTGTCTGC 
AGACACGTTT TGGATACACT CATTCAATTG GCCAAGGTAT TTCCCAGCCA CTTCACACAG 
CAGCGGACCA AAGAAACAAA CTGTGAGAGT GATCGGGAAA GGGGCAATAA GGCCTGTAGC 
CCATGCTCCT CACAGTCCTC CAGCAGTGGC ATTTGCACAG ACTTCTGGGA CTTATTGGTA 
AAACTGGACA ACATGAATGT CAGCCGGAAA GGCAAGAACT CCGTGAAGTC AGTGCCAGTG 
AGCGCTGGCG GTGAGGGGGA AACCTCTCCA TACAGCCTCG AGGCCTCTCC ACTGGGGCAG 
CTCATGAACA TGTTGTCACA CCCAGTCATC CGCCGGAGCT CTCTCTTAAC TGAGAAACTC 
CTCAGACTCC TTTCTCTCAT CTCAATTGCT CTCCCAGAAA ACAAGGTGTC AGAAGCACAG 
GCTAATTCTG GCAGCGGTGC TTCCTCCACC ACCACTGCCA CCTCAACCAC ATCTACCACC 
ACCACCACTG CCGCCTCCAC CACGCCCACA CCCCCTACTG CACCCACCCC TGTCACTTCT 
GCTCCAGCCC TGGTTGCTGC CACGGCTATT TCCACCATTG TCGTAGCTGC TTCGACCACA 
GTGACTACCC CCACGACTGC TACCACTACT GTTTCAATTT CTCCCACTAC TAAGGGCAGC 
AAATCTCCAG CGAAGGTGAG TGATGGGGGC AGCAGCAGTA CAGACTTTAA GATGGTGTCC 
TCTGGCCTCA CTGAAAACCA GCTACAGCTC TCTGTAGAGG TGTTGACATC CCACTCTTGT 
TCTGAGGAAG GCTTAGAGGA TGCAGCCAAC GTACTACTGC AGCTCTCCCG GGGGGACTCT 
GGGACCCGGG ACACTGTTCT CAAGCTGCTA CTGAATGGAG CCCGCCATCT GGGTTATACC 
CTTTGTAAAC AAATAGGTAC CCTGCTGGCC GAGCTGCGGG AATACAACCT CGAGCAGCAG 
CGGCGAGCCC AATGTGAAAC CCTCTCTCCT GATGGCCTGC CTGAGGAGCA GCCACAGACC 
ACCAAGCTGA AGGGCAAAAT GCAGAGCAGG TTTGACATGG CTGAGAATGT GGTAATTGTG 
GCATCTCAGA AGCGACCTTT GGGTGGCCGG GAGCTCCAGC TGCCTTCTAT GTCCATGTTG 
ACATCCAAGA CATCTACCCA GAAGTTCTTC TTGAGGGTAC TACAGGTCAT CATCCAGCTC 
CGGGACGACA CGCGCCGGGC TAACAAGAAA GCCAAGCAGA CAGGCAGGCT AGGTTCCTCC 
GGTTTAGGCT CAGCTAGCAG CATCCAGGCA GCTGTTCGGC AGCTGGAGGC TGAGGCTGAT 
GCCATTATAC AAATGTCGGA GTCTAGCCAG TCAGAGGCGT CTGTCCGGAG GGAGGAATCA 
CCCATGGATG TGGACCAGCC ATCTCCCAGT GCTCAAGATA CTCAATCCAT TGCCTCCGAT 
GGAACCCCAC AGGGGGAGAA GGAAAAGGAA GAAAGACCAC CTGAGTTACC CCTGCTCAGC 
GAGCAGCTGA GTTTGGACGA GCTGTGGGAC ATGCTTGGGG AGTGTCTAAA GGAACTAGAG 
GAATCCCATG ACCAGCATGC GGTGCTAGTG CTACAGCCTG CTGTCGAGGC CTTCTTTCTG 
GTCCATGCCA CAGAGCGGGA GAGCAAGCCT CCTGTCCGAG ACACCCGTGA GAGCCAGCTG 
GCACACATCA AGGACGAGCC TCCTCCACTC TCCCCTGCCC CCTTAACCCC AGCCACGCCT 
TCCTCCCTTG ACCCATTCTT CTCCCGGGAG CCCTCATCTA TGCACATCTC CTCAAGCCTG 
CCCCCTGACA CACAGAAGTT CCTTCGCTTT GCAGAGACTC ACCGCACTGT GTTAAACCAG 
ATCCTACGGC AGTCCACGAC CCACCTTGCT GATGGGCCTT TTGCTGTCCT GGTAGACTAC 
ATTCGTGTCC TCGACTTTGA TGTCAAGCGC AAATATTTCC GCCAAGAGCT GGAGCGTTTA 
GATGAGGGGC TCCGGAAAGA AGACATGGCT GTGCATGTCC GTCGTGACCA TGTGTTTGAA 
GACTCCTATC GTGAGCTGCA TCGCAAATCC CCCGAAGAAA TGAAGAATCG ATTGTATATA 
GTATTTGAAG GAGAAGAAGG GCAGGATGCT GGTGGGCTCC TGCGGGAGTG GTATATGATC 
ATCTCTCGAG AGATGTTTAA CCCTATGTAT GCCTTGTTCC GTACCTCACC TGGTGATCGA 
GTCACCTACA CCATCAATCC ATCTTCCCAC TGCAACCCCA ACCACCTCAG CTACTTCAAG 
TTTGTCGGAC GCATTGTGGC CAAAGCTGTA TATGACAACC GTCTTCTGGA GTGCTACTTT 
ACTCGATCCT TTTACAAACA CATCTTGGGC AAGTCAGTCA GATATACAGA TATGGAGAGT 
GAAGATTACC ACTTCTACCA AGGTCTGGTT TATCTGCTGG AAAATGATGT CTCCACACTA 
GGCTATGACC TCACCTTCAG CACTGAGGTC CAAGAGTTTG GAGTTTGTGA AGTTCGTGAC 
CTCAAACCCA ATGGGGCCAA CATCTTGGTA ACAGAGGAGA ATAAGAAGGA GTATGTACAC 
CTGGTATGCC AGATGAGAAT GACAGGAGCC ATCCGCAAGC AGTTGGCGGC TTTCTTAGAA 
GGCTTCTATG AGATCATTCC AAAGCGCCTC ATTTCCATCT TCACTGAGCA GGAGTTAGAG 
CTGCTTATAT CAGGACTGCC CACCATTGAC ATCGATGATC TGAAATCCAA CACTGAATAC 
CACAAGTACC AGTCCAACTC TATTCAGATC CAGTGGTTCT GGAGAGCATT GCGTTCTTTC 
GATCAAGCTG ACCGTGCCAA GTTCCTCCAG TTTGTCACAG GTACTTCCAA GGTACCCCTG 
CAAGGCTTTG CTGCCCTCGA AGGCATGAAT GGCATTCAGA AGTTTCAGAT CCATCGAGAT 
GACAGGTCCA CAGATCGCCT GCCTTCAGCT CACACATGTT TTAATCAGCT GGATCTGCCT 
GCCTATGAGA GCTTTGAGAA GCTCCGCCAC ATGCTACTGT TGGCTATCCA GGAGTGCTCT 
GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884683.1
CDS	3018..16478
Translation	MDPFISRREQKCMEIGTPRKNGVLVLRSSEAVYHLTTPFQIVCLHLPLSKSKPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029194.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029194.1

ORF Insert Sequence:

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GTTGCAGATG TGGCTATGGA AAGCAGTCCA GGCTCATCCA TCTCTATGGA GCACAGGCTG 
GATGTTGAAT TAAGGGCATC AGGTTCCAGC AGCAGCACTA ACATCTCTTC TGGCCCCAGC 
CCTGGTCCCA GTCCCGGCCC CGGCACCGGC CCTGGCCCCG GCCCCGGCCC CGGCCCCGGC 
CCTGGCCCCG GCCCCGGCCC CGGTCCTGGT CCCGGCCCTG GCCCCGGCCC TGGCCCTGGC 
CCCCGTCCTG GAGTCCAGTG TATTCCACAA CGAGCAGCAC TTCTGAAATC CATGTTGAAT 
TTCCTCAAGA AGGCCATCCA AGACCCTGCT TTCTCAGATG GCATACGACA TGTGATGGAT 
GGTTCTCTGC CTACCTCCCT GAAACACATC ATCAGCAATG CAGAATACTA TGGCCCATCA 
CTCTTCCTCC TAGCTACTGA AGTGGTGACT GTGTTTGTAT TTCAAGAACC ATCACTGCTC 
TCCTCACTCC AGGACAATGG ATTGACAGAT GTCATGCTGC ATGCACTGCT TATCAAAGAT 
GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC CTCCCAAATG TATTCAGTGC ACTCTGTTTG 
AATGCCCGAG GTCTTCAGTC TTTTGTTCAG TGTCAGCCTT TTGAACGCCT CTTCAAAGTT 
CTTCTGTCTC CAGATTACCT CCCAGCCATG CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG 
GATACTGCAT CCAACCTGGG GAGTGCTGTC GATGAGCTCA TGAGACATCA GCCCACCCTT 
AAAACAGATG CAACGACTGC CATCATCAAG TTACTTGAAG AAATCTGTAA TCTTGGAAGG 
GACCCCAAAT ACATCTGTCA GAAGCCATCA ATCCAGAAGG CAGATGGCAC TGCCACTGCT 
CCTCCCCCAA GGTCTAATCA TGCCGCAGAA GAAGCCTCTA GTGAGGATGA GGAGGAAGAG 
GAAGTACAGG CCATGCAGAG CTTTAATTCT ACCCAGCAAA ATGAAACTGA GCCTAATCAG 
CAGGTTGTTG GTACAGAGGA ACGTATTCCT ATTCCCCTCA TGGATTACAT CCTTAATGTG 
ATGAAATTTG TGGAATCTAT TCTGAGCAAC AATACAACAG ATGACCACTG CCAGGAATTT 
GTGAATCAGA AAGGACTGTT GCCTTTGGTT ACCATTTTGG GTCTTCCCAA TCTGCCCATT 
GACTTTCCCA CATCTGCTGC CTGTCAGGCT GTTGCAGGTG TCTGCAAATC CATATTGACA 
CTGTCACATG AACCCAAAGT CCTTCAAGAG GGTCTCCTTC AGTTGGACTC CATCCTCTCC 
TCCCTGGAGC CCTTACACCG CCCCATTGAA TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA 
CTGGCTTGCG CAGGCAATGT TGCTGATGCT ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG 
CATGCACTCA CTGCTGCCCA TGCCTACATC ATGATGTTTG TTCATACTTG CAGAGTTGGA 
CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC CAGTGGGGCT CTCAATTGGG TCTGAGTGTT 
TTGAGCAAGC TGAGCCAGTT ATACTGTTCC CTGGTGTGGG AAAGCACTGT CCTCCTCTCT 
CTGTGTACCC CAAACAGCCT ACCATCTGGG TGTGAATTTG GCCAGGCAGA TATGCAGAAA 
CTGGTTCCAA AGGATGAGAA GGCAGGTACG ACCCAGGGCG GAAAAAGATC AGATGGGGAA 
CAGGATGGAG CAGCTGGAAG TATGGATGCT TCTACCCAGG GCTTATTAGA AGGCATTGGG 
CTAGATGGTG ACACATTGGC TCCCATGGAG ACAGATGAAC CTACTGCTTC AGACTCTAAG 
GGCAAATCTA AAATCACACC AGCAATGGCT GCCAGAATTA AGCAAATCAA GCCTTTGTTA 
TCAGCTTCCT CCAGATTAGG CCGAGCACTT GCTGAGCTAT TTGGACTTCT TGTTAAACTT 
TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG AGCCATCATG CTGCCAGCAC CACTACAGCA 
CCGACACCTG CCGCGCGATC AACAGCCTCA GCTCTCACTA AGCTCTTGAC TAAGGGGTTA 
TCTTGGCAGC CCCCACCATA TACACCTACT CCCCGATTCA GGCTGACATT CTTCATCTGT 
TCAGTTGGTT TCACATCCCC AATGCTGTTT GATGAGAGGA AGTATCCCTA CCACCTCATG 
CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC AATGCTCTTT TTGAAACTTT CAACTGGGCT 
CTGTCCATGG GAGGTAAAGT TCCTGTTTCT GAGGGATTGG AACACTCAGA CTTGCCTGAT 
GGCACAGGAG AATTCCTAGA TGCCTGGCTT ATGCTGGTGG AGAAGATGGT GAATCCCACC 
ACGGTGCTTG AATCTCCACA TTCGCTGCCT GCCAAATTGC CTGGAGGTGT CCAGAACTTT 
CCCCAGTTCA GTGCACTGCG CTTCCTTGTG GTAACTCAGA AAGCAGCCTT TACTTGCATC 
AAAAACTTAT GGAACCGGAA ACCCCTGAAG GTATATGGTG GACGAATGGC TGAATCGATG 
CTGGCCATTC TATGCCACAT CCTCCGAGGA GAACCTGTGA TTCGAGAGAG ACTAAGCAAG 
GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG 
GAACCTCAAG TCAACCAGCA ACAACTGCAA CAGCTCATGG ACATGGGCTT CACAAGGGAA 
CATGCAATGG AGGCACTGTT GAACACCAGC ACCATGGAGC AGGCCACAGA GTACCTTTTA 
ACCCACCCTC CTCCAATCAT GGGAGGAGTT GTTCGGGATC TCAGCATGTC TGAAGAGGAC 
CAGATGATGA GAGCAATTGC TATGTCTCTG GGACAGGATA TTCCAATGGA TCAAAGGGCA 
GAGTCACCTG AGGAAGTTGC TTGCCGGAAG GAGGAAGAGG AACGGAAAGC TCGGGAAAAG 
CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG AAGTTCCAGG ATGCTGACCC GTTGGAACAA 
GATGAGCTCC ACACTTTCAC AGATACTATG TTGCCAGGCT GCTTCCACCT TCTTGATGAG 
CTGCCAGACA CAGTATACCG TGTGTGTGAC CTGATCATGA CAGCAATCAA ACGTAATGGA 
GCAGATTATC GTGACATGAT TCTGAAGCAA GTAGTCAATC AGGTGTGGGA AGCTGCTGAT 
GTATTGATCA AAGCTGCTCT TCCCCTGACA ACAAGTGACA CAAAAACCGT GTCAGAGTGG 
ATAAGTCAGA TGGCCACACT GCCCCAGGCC TCCAATTTGG CTACTAGAAT CTTGCTTTTA 
ACGCTACTTT TTGAGGAGTT GAAGCTACCT TGTGCTTGGG TGGTTGAATC AAGTGGCATC 
CTTAATGTCC TAATCAAACT CTTGGAAGTG GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG 
CAGAAGGAAG TCCAGACCCC AAAGTGGATC ACACCAGTGT TGCTCCTGAT TGATTTCTAT 
GAAAAGACAG CCATCTCCTC AAAAAGGAGA GCCCAGATGA CTAAGTACCT GCAATCCAAC 
AGCAACAACT GGCGCTGGTT TGATGATCGC TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC 
AACAATAGCA CTATTGATTC TGCCTGGAAA TCTGGAGAGA CAAGCGTGCG ATTCACTGCA 
GGCCGAAGAA GATACACGGT CCAATTCACT ACAATGGTGC AGGTTAATGA GGAAACAGGG 
AACCGACGCC CTGTGATGCT GACTCTCCTC AGGGTACCTC GGCTGAATAA AAATTCAAAA 
AACAGCAATG GACAGGAACT AGAGAAGACG CTGGAAGAAA GCAAAGAAAT GGATATCAAA 
CGTAAAGAAA ATAAAGGCAA TGATACCCCT TTGGCCCTAG AGAGTACAAA CACTGAAAAG 
GAGACAAGCC TGGAGGAAAC AAAAATCGGG GAGATCCTGA TCCAGGGCTT GACAGAAGAT 
ATGGTGACTG TTTTAATCCG GGCCTGCGTG AGCATGCTGG GAGTCCCTGT GGACCCAGAT 
ACTTTGCATG CCACCCTTCG TCTCTGTCTG AGGCTCACCC GGGACCACAA ATATGCCATG 
ATGTTTGCAG AACTGAAGAG TACCCGCATG ATCTTGAATT TGACCCAGAG CTCAGGCTTC 
AATGGGTTTA CTCCCCTGGT CACCCTTCTC TTAAGACACA TCATTGAGGA CCCCTGTACC 
CTTCGTCATA CCATGGAAAA GGTTGTTCGC TCAGCAGCTA CAAGTGGAGC TGGTAGCACT 
ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC TCTCGGGAGA TCAACTACAT CCTTCGTGTC 
CTTGGGCCAG CCGCATGCCG CAATCCAGAC ATATTCACAG AAGTGGCCAA CTGCTGTATC 
CGCATCGCCC TTCCTGCCCC TCGAGGCTCA GGAACTGCTT CAGATGATGA ATTTGAGAAT 
CTTAGAATTA AAGGCCCTAA TGCTGTACAG CTGGTGAAGA CCACCCCTTT GAAGCCCTCA 
CCTCTGCCTG TCATCCCTGA TACTATCAAG GAAGTGATCT ATGATATGCT GAATGCTCTG 
GCTGCATACC ATGCTCCAGA GGAAGCAGAT AAATCTGATC CTAAACCTGG GGTTATGACC 
CAAGAGGTTG GCCAGCTCCT GCAAGACATG GGTGATGATG TATACCAGCA GTACCGGTCA 
CTTACGCGTC AGAGCAGTGA CTTTGATACG CAGTCAGGTT TTTCCATTAA TAGTCAGGTC 
TTTGCTGCAG ATGGTGCCTC CACTGAGACT TCCGCATCTG GGACCTCCCA AGGAGAGGCT 
TCAACTCCAG AGGAGTCTCG AGATGGGAAG AAAGATAAAG AAGGGGACCG GGCCTCTGAG 
GAAGGCAAAC AGAAAGGCAA GGGCAGCAAA CCTTTAATGC CTACCTCCAC TATCCTTCGT 
CTTCTGGCAG AGTTGGTGAG GTCCTATGTT GGTATTGCTA CCCTGATTGC CAACTACAGC 
TACACTGTGG GCCAGTCTGA ACTGATCAAA GAGGACTGCA GTGTGCTAGC TTTTGTTCTG 
GACCACCTGC TCCCACATAC CCAGAATGCA GAAGACAAGG ACACCCCTGC CTTGGCCCGC 
CTGTTCCTCG CAAGCCTGGC TGCTGCAGGG AGTGGCACAG ATGCCCAGGT GGCCCTAGTG 
AATGAAGTAA AAGCAGCCCT TGGACGGGCA CTGGCTATGG CTGAGAGTAC AGAGAAACAT 
GCCAGGCTTC AGGCAGTGAT GTGTATCATC AGTACTATCA TGGAGTCCTG CCCCTCCACC 
TCCAGCTTCT ACAGCAGTGC CACAGCGAAG ACCCAGCACA ATGGCATGAA CAACATCATT 
CGGCTTTTCC TGAAGAAGGG ACTGGTTAAT GACCTGGCCA GAGTACCTCA CAGCTTAGAC 
CTGTCCAGTC CCAACATGGC CAACACAGTC AATGCTGCTC TGAAGCCTTT GGAAACACTT 
TCCCGGATTG TGAACCAGCC CAGTAGCCTT TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC 
AAGTCTGAGC AGGATGCCCA AGGAGCCTCT CAAGATTCCA GTAGCAACCA GCAGGACCCA 
GGCGAGCCTG GGGAAGCAGA AGTGCAGGAG GAGGATCATG ATGTCACTCA GACAGAGGTG 
GCAGATGGGG ATATCATGGA TGGGGAGGCT GAAACCGACT CAGTGGTGAT TGCTGGGCAG 
CCTGAGGTGC TCAGTTCACA AGAGATGCAG GTTGAGAATG AGCTGGAGGA CCTGATAGAT 
GAGTTGCTTG AGAGGGATGG CGGATCTGGG AACAGTACAA TTATAGTGAG CAGAAGTGGA 
GAGGATGAAT CACAAGAGGA CGTGCTGATG GATGAAGCTC CTTCCAACCT CAGCCAAGCT 
TCCACCTTGC AGGCCAACCG AGAAGATTCC ATGAATATCC TGGACCCTGA GGATGAGGAG 
GAGCACACTC AGGAAGAGGA CAGCAGTGGC AGTAACGAGG ATGAGGATGA TAGTCAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGAGGAA GATGATCAGG AGGATGATGA AGGTGAAGAG 
GGAGATGAAG ACGATGACGA CGATGGCTCT GAGATGGAAT TGGATGAGGA TTATCCTGAT 
ATGAACGCTT CTCCCTTGGT CCGATTTGAG CGCTTTGACC GGGAGGATGA TCTCATCATT 
GAGTTTGACA ACATGTTCTC CAGTGCTACA GACATCCCCC CATCCCCAGG AAATATCCCT 
ACCACCCATC CACTGATGGT GCGCCATGCA GACCACAGTT CTCTGACACT GGGCAGTGGC 
TCTTCAACAA CTCGTCTCAC CCAGGGCATC GGGCGCAGTC AGAGGACCCT AAGGCAGCTG 
ACGGCCAATA CTGGCCACAC CATTCATGTT CACTACCCTG GGAATCGCCA GCCCAACCCT 
CCTCTTATAC TGCAGAGGTT GCTTGGTCCC TCAGCTGCTG CTGACATCCT TCAGCTGAGC 
AGCAGCCTTC CCCTACAAAG CCGGGGTCGG GCCCGCCTCC TGGTAGGCAA CGATGACGTC 
CACATCATCG CCCGTTCTGA TGATGAGCTG CTGGATGACT TTTTCCATGA TCAGAGCACA 
GCTACCAGCC AAGCAGGAAC CCTGTCCAGC ATCCCCACAG CCCTGACCCG CTGGACAGAA 
GAATGCAAAG TTCTCGATGC TGAGAGCATG CATGACTGTG TTTCAGTGGT TAAAGTGTCC 
ATTGTCAATC ACCTGGAATT CCTGAGGGAT GAGGAGCTGG AAGAAAGGCG AGAGAAGCGC 
AGGAAACAAC TGGCTGAGGA AGAAACAAAG ATAACTGATA AAGGCAAAGA AGATAAGGAG 
AACAGGGATC AGAGTGCACA GTGTACTGCA TCTAAGTCAA ATGACTCCAC TGAACAGAAT 
CTCTCAGATG GGACGCCTAT GCCTGACAGC TACCCAACAA CCCCATCTTC AACTGATGCA 
GCTACATCTG AGTCCAAGGA GACCCTTGGC ACTCTGCAAT CCTCACAACA GCAACCAACA 
CTCCCAACCC CACCAGCTTT GGGAGAGGTT CCTCAGGAGC TGCAGTCTCC AGCTGGAGAA 
GGGGGCAGCT CTACACAGCT ATTGATGCCT GTAGAGCCAG AGGAATTGGG TCCCACAAGG 
CCAAGTGGGG AAGCAGAAAC AACTCAGATG GAGTTATCCC CAGCTCCCAC TATAACCTCA 
CTTTCCCCAG AGAGAGCTGA GGATTCTGAT GCACTGACGG CTGTCAGCAG TCAGCTAGAA 
GGCTCTCCTA TGGATACAAG CAGCCTGGCT TCCTGTACCT TAGAGGAGGC TGTGGGTGAC 
ACTTCAGCAG CTGGCAGTTC TGAGCAGCCC AGAGCAGGCA GCTCCACTCC TGGGGATGCC 
CCACCAGCTG TGGCGGAAGT GCAAGGCAGG AGTGATGGGT CAGGGGAATC TGCCCAGCCA 
CCTGAGGACA GCTCCCCACC TGCATCCTCT GAGAGCTCTT CCACCAGAGA TTCTGCCGTG 
GCCATTTCTG GAGCAGATTC CCGAGGAATC CTAGAAGAGC CGTTGCCTTC AACAAGCAGT 
GAAGAAGAAG ATCCCCTTGC GGGTATCAGT CTCCCTGAAG GTGTGGACCC CTCTTTTCTG 
GCTGCCCTGC CTGATGACAT CCGTCGGGAA GTTCTACAGA ACCAGCTAGG CATTCGTCCA 
CCAACCCGGA CTGCCCCCTC CACAAATAGC TCAGCGCCTG CAGTGGTGGG GAATCCTGGT 
GTGACTGAAG TGAGCCCTGA GTTTCTGGCT GCCCTGCCTC CAGCCATTCA GGAGGAAGTA 
CTGGCACAGC AGAGAGCTGA GCAGCAGCGA CGAGAACTAG CACAGAATGC CAGCTCAGAC 
ACCCCTATGG ACCCTGTGAC CTTCATCCAG ACTCTGCCCT CAGACCTGCG CCGTAGTGTC 
CTAGAGGATA TGGAGGACAG TGTGTTAGCT GTGATGCCAC CTGACATTGC AGCTGAGGCT 
CAAGCCCTGA GACGAGAGCA AGAAGCCCGG CAGCGACAGC TCATGCATGA GCGTCTGTTT 
GGGCACAGTA GCACCTCCGC ACTCTCTGCT ATTCTCCGAA GCCCGGCTTT CACCAGTCGC 
TTAAGTGGCA ACCGTGGGGT CCAGTATACT CGCCTTGCTG TGCAGAGAGG TGGCACCTTC 
CAGATGGGGG GTAGCAGCAG CCATAACAGG CCTTCTGGCA GTAATGTAGA TACTCTCCTC 
CGCCTCCGAG GACGGCTCCT TCTGGACCAC GAAGCCCTTT CTTGTCTCTT GGTCCTACTT 
TTTGTGGATG AGCCAAAGCT CAATACTAGC CGTCTACACC GAGTACTGAG AAATCTCTGC 
TACCATGCCC AGACCCGCCA CTGGGTCATC CGCAGTCTGC TCTCCATCTT GCAGCGCAGC 
AGTGAGAGTG AGCTATGCAT TGAAACACCC AAACTCACTA CAAGTGAGGA AAAGGGCAAA 
AAGTCGAGCA AGAGCTGTGG GTCAAGTAGC CATGAGAACC GTCCCCTGGA CCTGCTACAC 
AAGATGGAGT CAAAGAGCTC CAACCAGCTT TCCTGGCTCT CAGTATCCAT GGATGCAGCC 
CTAGGCTGCA GGACTAATAT ATTTCAGATC CAGCGTTCAG GGGGGCGTAA ACATACCGAG 
AAGCATGCAA GCGGTGGCTC CACCGTCCAC ATCCATCCCC AAGCTGCTCC TGTTGTCTGC 
AGACACGTTT TGGATACACT CATTCAATTG GCCAAGGTAT TTCCCAGCCA CTTCACACAG 
CAGCGGACCA AAGAAACAAA CTGTGAGAGT GATCGGGAAA GGGGCAATAA GGCCTGTAGC 
CCATGCTCCT CACAGTCCTC CAGCAGTGGC ATTTGCACAG ACTTCTGGGA CTTATTGGTA 
AAACTGGACA ACATGAATGT CAGCCGGAAA GGCAAGAACT CCGTGAAGTC AGTGCCAGTG 
AGCGCTGGCG GTGAGGGGGA AACCTCTCCA TACAGCCTCG AGGCCTCTCC ACTGGGGCAG 
CTCATGAACA TGTTGTCACA CCCAGTCATC CGCCGGAGCT CTCTCTTAAC TGAGAAACTC 
CTCAGACTCC TTTCTCTCAT CTCAATTGCT CTCCCAGAAA ACAAGGTGTC AGAAGCACAG 
GCTAATTCTG GCAGCGGTGC TTCCTCCACC ACCACTGCCA CCTCAACCAC ATCTACCACC 
ACCACCACTG CCGCCTCCAC CACGCCCACA CCCCCTACTG CACCCACCCC TGTCACTTCT 
GCTCCAGCCC TGGTTGCTGC CACGGCTATT TCCACCATTG TCGTAGCTGC TTCGACCACA 
GTGACTACCC CCACGACTGC TACCACTACT GTTTCAATTT CTCCCACTAC TAAGGGCAGC 
AAATCTCCAG CGAAGGTGAG TGATGGGGGC AGCAGCAGTA CAGACTTTAA GATGGTGTCC 
TCTGGCCTCA CTGAAAACCA GCTACAGCTC TCTGTAGAGG TGTTGACATC CCACTCTTGT 
TCTGAGGAAG GCTTAGAGGA TGCAGCCAAC GTACTACTGC AGCTCTCCCG GGGGGACTCT 
GGGACCCGGG ACACTGTTCT CAAGCTGCTA CTGAATGGAG CCCGCCATCT GGGTTATACC 
CTTTGTAAAC AAATAGGTAC CCTGCTGGCC GAGCTGCGGG AATACAACCT CGAGCAGCAG 
CGGCGAGCCC AATGTGAAAC CCTCTCTCCT GATGGCCTGC CTGAGGAGCA GCCACAGACC 
ACCAAGCTGA AGGGCAAAAT GCAGAGCAGG TTTGACATGG CTGAGAATGT GGTAATTGTG 
GCATCTCAGA AGCGACCTTT GGGTGGCCGG GAGCTCCAGC TGCCTTCTAT GTCCATGTTG 
ACATCCAAGA CATCTACCCA GAAGTTCTTC TTGAGGGTAC TACAGGTCAT CATCCAGCTC 
CGGGACGACA CGCGCCGGGC TAACAAGAAA GCCAAGCAGA CAGGCAGGCT AGGTTCCTCC 
GGTTTAGGCT CAGCTAGCAG CATCCAGGCA GCTGTTCGGC AGCTGGAGGC TGAGGCTGAT 
GCCATTATAC AAATGTCGGA GTCTAGCCAG TCAGAGGCGT CTGTCCGGAG GGAGGAATCA 
CCCATGGATG TGGACCAGCC ATCTCCCAGT GCTCAAGATA CTCAATCCAT TGCCTCCGAT 
GGAACCCCAC AGGGGGAGAA GGAAAAGGAA GAAAGACCAC CTGAGTTACC CCTGCTCAGC 
GAGCAGCTGA GTTTGGACGA GCTGTGGGAC ATGCTTGGGG AGTGTCTAAA GGAACTAGAG 
GAATCCCATG ACCAGCATGC GGTGCTAGTG CTACAGCCTG CTGTCGAGGC CTTCTTTCTG 
GTCCATGCCA CAGAGCGGGA GAGCAAGCCT CCTGTCCGAG ACACCCGTGA GAGCCAGCTG 
GCACACATCA AGGACGAGCC TCCTCCACTC TCCCCTGCCC CCTTAACCCC AGCCACGCCT 
TCCTCCCTTG ACCCATTCTT CTCCCGGGAG CCCTCATCTA TGCACATCTC CTCAAGCCTG 
CCCCCTGACA CACAGAAGTT CCTTCGCTTT GCAGAGACTC ACCGCACTGT GTTAAACCAG 
ATCCTACGGC AGTCCACGAC CCACCTTGCT GATGGGCCTT TTGCTGTCCT GGTAGACTAC 
ATTCGTGTCC TCGACTTTGA TGTCAAGCGC AAATATTTCC GCCAAGAGCT GGAGCGTTTA 
GATGAGGGGC TCCGGAAAGA AGACATGGCT GTGCATGTCC GTCGTGACCA TGTGTTTGAA 
GACTCCTATC GTGAGCTGCA TCGCAAATCC CCCGAAGAAA TGAAGAATCG ATTGTATATA 
GTATTTGAAG GAGAAGAAGG GCAGGATGCT GGTGGGCTCC TGCGGGAGTG GTATATGATC 
ATCTCTCGAG AGATGTTTAA CCCTATGTAT GCCTTGTTCC GTACCTCACC TGGTGATCGA 
GTCACCTACA CCATCAATCC ATCTTCCCAC TGCAACCCCA ACCACCTCAG CTACTTCAAG 
TTTGTCGGAC GCATTGTGGC CAAAGCTGTA TATGACAACC GTCTTCTGGA GTGCTACTTT 
ACTCGATCCT TTTACAAACA CATCTTGGGC AAGTCAGTCA GATATACAGA TATGGAGAGT 
GAAGATTACC ACTTCTACCA AGGTCTGGTT TATCTGCTGG AAAATGATGT CTCCACACTA 
GGCTATGACC TCACCTTCAG CACTGAGGTC CAAGAGTTTG GAGTTTGTGA AGTTCGTGAC 
CTCAAACCCA ATGGGGCCAA CATCTTGGTA ACAGAGGAGA ATAAGAAGGA GTATGTACAC 
CTGGTATGCC AGATGAGAAT GACAGGAGCC ATCCGCAAGC AGTTGGCGGC TTTCTTAGAA 
GGCTTCTATG AGATCATTCC AAAGCGCCTC ATTTCCATCT TCACTGAGCA GGAGTTAGAG 
CTGCTTATAT CAGGACTGCC CACCATTGAC ATCGATGATC TGAAATCCAA CACTGAATAC 
CACAAGTACC AGTCCAACTC TATTCAGATC CAGTGGTTCT GGAGAGCATT GCGTTCTTTC 
GATCAAGCTG ACCGTGCCAA GTTCCTCCAG TTTGTCACAG GTACTTCCAA GGTACCCCTG 
CAAGGCTTTG CTGCCCTCGA AGGCATGAAT GGCATTCAGA AGTTTCAGAT CCATCGAGAT 
GACAGGTCCA CAGATCGCCT GCCTTCAGCT CACACATGTT TTAATCAGCT GGATCTGCCT 
GCCTATGAGA GCTTTGAGAA GCTCCGCCAC ATGCTACTGT TGGCTATCCA GGAGTGCTCT 
GAAGGCTTTG GGCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029195.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884684.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X5, mRNA.

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CloneID	OHu99116
Clone ID Related Accession (Same CDS sequence)	XM_017029195.1
Accession Version	XM_017029195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GTTGCAGATG TGGCTATGGA AAGCAGTCCA GGCTCATCCA TCTCTATGGA GCACAGGCTG 
GATGTTGAAT TAAGGGCATC AGGTTCCAGC AGCAGCACTA ACATCTCTTC TGGCCCCAGC 
CCTGGTCCCA GTCCCGGCCC CGGCACCGGC CCTGGCCCCG GCCCCGGCCC CGGCCCCGGC 
CCTGGCCCCG GCCCCGGCCC CGGTCCTGGT CCCGGCCCTG GCCCCGGCCC TGGCCCTGGC 
CCCCGTCCTG GAGTCCAGTG TATTCCACAA CGAGCAGCAC TTCTGAAATC CATGTTGAAT 
TTCCTCAAGA AGGCCATCCA AGACCCTGCT TTCTCAGATG GCATACGACA TGTGATGGAT 
GGTTCTCTGC CTACCTCCCT GAAACACATC ATCAGCAATG CAGAATACTA TGGCCCATCA 
CTCTTCCTCC TAGCTACTGA AGTGGTGACT GTGTTTGTAT TTCAAGAACC ATCACTGCTC 
TCCTCACTCC AGGACAATGG ATTGACAGAT GTCATGCTGC ATGCACTGCT TATCAAAGAT 
GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC CTCCCAAATG TATTCAGTGC ACTCTGTTTG 
AATGCCCGAG GTCTTCAGTC TTTTGTTCAG TGTCAGCCTT TTGAACGCCT CTTCAAAGTT 
CTTCTGTCTC CAGATTACCT CCCAGCCATG CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG 
GATACTGCAT CCAACCTGGG GAGTGCTGTC GATGAGCTCA TGAGACATCA GCCCACCCTT 
AAAACAGATG CAACGACTGC CATCATCAAG TTACTTGAAG AAATCTGTAA TCTTGGAAGG 
GACCCCAAAT ACATCTGTCA GAAGCCATCA ATCCAGAAGG CAGATGGCAC TGCCACTGCT 
CCTCCCCCAA GGTCTAATCA TGCCGCAGAA GAAGCCTCTA GTGAGGATGA GGAGGAAGAG 
GAAGTACAGG CCATGCAGAG CTTTAATTCT ACCCAGCAAA ATGAAACTGA GCCTAATCAG 
CAGGTTGTTG GTACAGAGGA ACGTATTCCT ATTCCCCTCA TGGATTACAT CCTTAATGTG 
ATGAAATTTG TGGAATCTAT TCTGAGCAAC AATACAACAG ATGACCACTG CCAGGAATTT 
GTGAATCAGA AAGGACTGTT GCCTTTGGTT ACCATTTTGG GTCTTCCCAA TCTGCCCATT 
GACTTTCCCA CATCTGCTGC CTGTCAGGCT GTTGCAGGTG TCTGCAAATC CATATTGACA 
CTGTCACATG AACCCAAAGT CCTTCAAGAG GGTCTCCTTC AGTTGGACTC CATCCTCTCC 
TCCCTGGAGC CCTTACACCG CCCCATTGAA TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA 
CTGGCTTGCG CAGGCAATGT TGCTGATGCT ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG 
CATGCACTCA CTGCTGCCCA TGCCTACATC ATGATGTTTG TTCATACTTG CAGAGTTGGA 
CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC CAGTGGGGCT CTCAATTGGG TCTGAGTGTT 
TTGAGCAAGC TGAGCCAGTT ATACTGTTCC CTGGTGTGGG AAAGCACTGT CCTCCTCTCT 
CTGTGTACCC CAAACAGCCT ACCATCTGGG TGTGAATTTG GCCAGGCAGA TATGCAGAAA 
CTGGTTCCAA AGGATGAGAA GGCAGGTACG ACCCAGGGCG GAAAAAGATC AGATGGGGAA 
CAGGATGGAG CAGCTGGAAG TATGGATGCT TCTACCCAGG GCTTATTAGA AGGCATTGGG 
CTAGATGGTG ACACATTGGC TCCCATGGAG ACAGATGAAC CTACTGCTTC AGACTCTAAG 
GGCAAATCTA AAATCACACC AGCAATGGCT GCCAGAATTA AGCAAATCAA GCCTTTGTTA 
TCAGCTTCCT CCAGATTAGG CCGAGCACTT GCTGAGCTAT TTGGACTTCT TGTTAAACTT 
TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG AGCCATCATG CTGCCAGCAC CACTACAGCA 
CCGACACCTG CCGCGCGATC AACAGCCTCA GCTCTCACTA AGCTCTTGAC TAAGGGGTTA 
TCTTGGCAGC CCCCACCATA TACACCTACT CCCCGATTCA GGCTGACATT CTTCATCTGT 
TCAGTTGGTT TCACATCCCC AATGCTGTTT GATGAGAGGA AGTATCCCTA CCACCTCATG 
CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC AATGCTCTTT TTGAAACTTT CAACTGGGCT 
CTGTCCATGG GAGGTAAAGT TCCTGTTTCT GAGGGATTGG AACACTCAGA CTTGCCTGAT 
GGCACAGGAG AATTCCTAGA TGCCTGGCTT ATGCTGGTGG AGAAGATGGT GAATCCCACC 
ACGGTGCTTG AATCTCCACA TTCGCTGCCT GCCAAATTGC CTGGAGGTGT CCAGAACTTT 
CCCCAGTTCA GTGCACTGCG CTTCCTTGTG GTAACTCAGA AAGCAGCCTT TACTTGCATC 
AAAAACTTAT GGAACCGGAA ACCCCTGAAG GTATATGGTG GACGAATGGC TGAATCGATG 
CTGGCCATTC TATGCCACAT CCTCCGAGGA GAACCTGTGA TTCGAGAGAG ACTAAGCAAG 
GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG 
GAACCTCAAG TCAACCAGCA ACAACTGCAA CAGCTCATGG ACATGGGCTT CACAAGGGAA 
CATGCAATGG AGGCACTGTT GAACACCAGC ACCATGGAGC AGGCCACAGA GTACCTTTTA 
ACCCACCCTC CTCCAATCAT GGGAGGAGTT GTTCGGGATC TCAGCATGTC TGAAGAGGAC 
CAGATGATGA GAGCAATTGC TATGTCTCTG GGACAGGATA TTCCAATGGA TCAAAGGGCA 
GAGTCACCTG AGGAAGTTGC TTGCCGGAAG GAGGAAGAGG AACGGAAAGC TCGGGAAAAG 
CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG AAGTTCCAGG ATGCTGACCC GTTGGAACAA 
GATGAGCTCC ACACTTTCAC AGATACTATG TTGCCAGGCT GCTTCCACCT TCTTGATGAG 
CTGCCAGACA CAGTATACCG TGTGTGTGAC CTGATCATGA CAGCAATCAA ACGTAATGGA 
GCAGATTATC GTGACATGAT TCTGAAGCAA GTAGTCAATC AGGTGTGGGA AGCTGCTGAT 
GTATTGATCA AAGCTGCTCT TCCCCTGACA ACAAGTGACA CAAAAACCGT GTCAGAGTGG 
ATAAGTCAGA TGGCCACACT GCCCCAGGCC TCCAATTTGG CTACTAGAAT CTTGCTTTTA 
ACGCTACTTT TTGAGGAGTT GAAGCTACCT TGTGCTTGGG TGGTTGAATC AAGTGGCATC 
CTTAATGTCC TAATCAAACT CTTGGAAGTG GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG 
CAGAAGGAAG TCCAGACCCC AAAGTGGATC ACACCAGTGT TGCTCCTGAT TGATTTCTAT 
GAAAAGACAG CCATCTCCTC AAAAAGGAGA GCCCAGATGA CTAAGTACCT GCAATCCAAC 
AGCAACAACT GGCGCTGGTT TGATGATCGC TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC 
AACAATAGCA CTATTGATTC TGCCTGGAAA TCTGGAGAGA CAAGCGTGCG ATTCACTGCA 
GGCCGAAGAA GATACACGGT CCAATTCACT ACAATGGTGC AGGTTAATGA GGAAACAGGG 
AACCGACGCC CTGTGATGCT GACTCTCCTC AGGGTACCTC GGCTGAATAA AAATTCAAAA 
AACAGCAATG GACAGGAACT AGAGAAGACG CTGGAAGAAA GCAAAGAAAT GGATATCAAA 
CGTAAAGAAA ATAAAGGCAA TGATACCCCT TTGGCCCTAG AGAGTACAAA CACTGAAAAG 
GAGACAAGCC TGGAGGAAAC AAAAATCGGG GAGATCCTGA TCCAGGGCTT GACAGAAGAT 
ATGGTGACTG TTTTAATCCG GGCCTGCGTG AGCATGCTGG GAGTCCCTGT GGACCCAGAT 
ACTTTGCATG CCACCCTTCG TCTCTGTCTG AGGCTCACCC GGGACCACAA ATATGCCATG 
ATGTTTGCAG AACTGAAGAG TACCCGCATG ATCTTGAATT TGACCCAGAG CTCAGGCTTC 
AATGGGTTTA CTCCCCTGGT CACCCTTCTC TTAAGACACA TCATTGAGGA CCCCTGTACC 
CTTCGTCATA CCATGGAAAA GGTTGTTCGC TCAGCAGCTA CAAGTGGAGC TGGTAGCACT 
ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC TCTCGGGAGA TCAACTACAT CCTTCGTGTC 
CTTGGGCCAG CCGCATGCCG CAATCCAGAC ATATTCACAG AAGTGGCCAA CTGCTGTATC 
CGCATCGCCC TTCCTGCCCC TCGAGGCTCA GGAACTGCTT CAGATGATGA ATTTGAGAAT 
CTTAGAATTA AAGGCCCTAA TGCTGTACAG CTGGTGAAGA CCACCCCTTT GAAGCCCTCA 
CCTCTGCCTG TCATCCCTGA TACTATCAAG GAAGTGATCT ATGATATGCT GAATGCTCTG 
GCTGCATACC ATGCTCCAGA GGAAGCAGAT AAATCTGATC CTAAACCTGG GGTTATGACC 
CAAGAGGTTG GCCAGCTCCT GCAAGACATG GGTGATGATG TATACCAGCA GTACCGGTCA 
CTTACGCGTC AGAGCAGTGA CTTTGATACG CAGTCAGGTT TTTCCATTAA TAGTCAGGTC 
TTTGCTGCAG ATGGTGCCTC CACTGAGACT TCCGCATCTG GGACCTCCCA AGGAGAGGCT 
TCAACTCCAG AGGAGTCTCG AGATGGGAAG AAAGATAAAG AAGGGGACCG GGCCTCTGAG 
GAAGGCAAAC AGAAAGGCAA GGGCAGCAAA CCTTTAATGC CTACCTCCAC TATCCTTCGT 
CTTCTGGCAG AGTTGGTGAG GTCCTATGTT GGTATTGCTA CCCTGATTGC CAACTACAGC 
TACACTGTGG GCCAGTCTGA ACTGATCAAA GAGGACTGCA GTGTGCTAGC TTTTGTTCTG 
GACCACCTGC TCCCACATAC CCAGAATGCA GAAGACAAGG ACACCCCTGC CTTGGCCCGC 
CTGTTCCTCG CAAGCCTGGC TGCTGCAGGG AGTGGCACAG ATGCCCAGGT GGCCCTAGTG 
AATGAAGTAA AAGCAGCCCT TGGACGGGCA CTGGCTATGG CTGAGAGTAC AGAGAAACAT 
GCCAGGCTTC AGGCAGTGAT GTGTATCATC AGTACTATCA TGGAGTCCTG CCCCTCCACC 
TCCAGCTTCT ACAGCAGTGC CACAGCGAAG ACCCAGCACA ATGGCATGAA CAACATCATT 
CGGCTTTTCC TGAAGAAGGG ACTGGTTAAT GACCTGGCCA GAGTACCTCA CAGCTTAGAC 
CTGTCCAGTC CCAACATGGC CAACACAGTC AATGCTGCTC TGAAGCCTTT GGAAACACTT 
TCCCGGATTG TGAACCAGCC CAGTAGCCTT TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC 
AAGTCTGAGC AGGATGCCCA AGGAGCCTCT CAAGATTCCA GTAGCAACCA GCAGGACCCA 
GGCGAGCCTG GGGAAGCAGA AGTGCAGGAG GAGGATCATG ATGTCACTCA GACAGAGGTG 
GCAGATGGGG ATATCATGGA TGGGGAGGCT GAAACCGACT CAGTGGTGAT TGCTGGGCAG 
CCTGAGGTGC TCAGTTCACA AGAGATGCAG GTTGAGAATG AGCTGGAGGA CCTGATAGAT 
GAGTTGCTTG AGAGGGATGG CGGATCTGGG AACAGTACAA TTATAGTGAG CAGAAGTGGA 
GAGGATGAAT CACAAGAGGA CGTGCTGATG GATGAAGCTC CTTCCAACCT CAGCCAAGCT 
TCCACCTTGC AGGCCAACCG AGAAGATTCC ATGAATATCC TGGACCCTGA GGATGAGGAG 
GAGCACACTC AGGAAGAGGA CAGCAGTGGC AGTAACGAGG ATGAGGATGA TAGTCAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGAGGAA GATGATCAGG AGGATGATGA AGGTGAAGAG 
GGAGATGAAG ACGATGACGA CGATGGCTCT GAGATGGAAT TGGATGAGGA TTATCCTGAT 
ATGAACGCTT CTCCCTTGGT CCGATTTGAG CGCTTTGACC GGGAGGATGA TCTCATCATT 
GAGTTTGACA ACATGTTCTC CAGTGCTACA GACATCCCCC CATCCCCAGG AAATATCCCT 
ACCACCCATC CACTGATGGT GCGCCATGCA GACCACAGTT CTCTGACACT GGGCAGTGGC 
TCTTCAACAA CTCGTCTCAC CCAGGGCATC GGGCGCAGTC AGAGGACCCT AAGGCAGCTG 
ACGGCCAATA CTGGCCACAC CATTCATGTT CACTACCCTG GGAATCGCCA GCCCAACCCT 
CCTCTTATAC TGCAGAGGTT GCTTGGTCCC TCAGCTGCTG CTGACATCCT TCAGCTGAGC 
AGCAGCCTTC CCCTACAAAG CCGGGGTCGG GCCCGCCTCC TGGTAGGCAA CGATGACGTC 
CACATCATCG CCCGTTCTGA TGATGAGCTG CTGGATGACT TTTTCCATGA TCAGAGCACA 
GCTACCAGCC AAGCAGGAAC CCTGTCCAGC ATCCCCACAG CCCTGACCCG CTGGACAGAA 
GAATGCAAAG TTCTCGATGC TGAGAGCATG CATGACTGTG TTTCAGTGGT TAAAGTGTCC 
ATTGTCAATC ACCTGGAATT CCTGAGGGAT GAGGAGCTGG AAGAAAGGCG AGAGAAGCGC 
AGGAAACAAC TGGCTGAGGA AGAAACAAAG ATAACTGATA AAGGCAAAGA AGATAAGGAG 
AACAGGGATC AGAGTGCACA GTGTACTGCA TCTAAGTCAA ATGACTCCAC TGAACAGAAT 
CTCTCAGATG GGACGCCTAT GCCTGACAGC TACCCAACAA CCCCATCTTC AACTGATGCA 
GCTACATCTG AGTCCAAGGA GACCCTTGGC ACTCTGCAAT CCTCACAACA GCAACCAACA 
CTCCCAACCC CACCAGCTTT GGGAGAGGTT CCTCAGGAGC TGCAGTCTCC AGCTGGAGAA 
GGGGGCAGCT CTACACAGCT ATTGATGCCT GTAGAGCCAG AGGAATTGGG TCCCACAAGG 
CCAAGTGGGG AAGCAGAAAC AACTCAGATG GAGTTATCCC CAGCTCCCAC TATAACCTCA 
CTTTCCCCAG AGAGAGCTGA GGATTCTGAT GCACTGACGG CTGTCAGCAG TCAGCTAGAA 
GGCTCTCCTA TGGATACAAG CAGCCTGGCT TCCTGTACCT TAGAGGAGGC TGTGGGTGAC 
ACTTCAGCAG CTGGCAGTTC TGAGCAGCCC AGAGCAGGCA GCTCCACTCC TGGGGATGCC 
CCACCAGCTG TGGCGGAAGT GCAAGGCAGG AGTGATGGGT CAGGGGAATC TGCCCAGCCA 
CCTGAGGACA GCTCCCCACC TGCATCCTCT GAGAGCTCTT CCACCAGAGA TTCTGCCGTG 
GCCATTTCTG GAGCAGATTC CCGAGGAATC CTAGAAGAGC CGTTGCCTTC AACAAGCAGT 
GAAGAAGAAG ATCCCCTTGC GGGTATCAGT CTCCCTGAAG GTGTGGACCC CTCTTTTCTG 
GCTGCCCTGC CTGATGACAT CCGTCGGGAA GTTCTACAGA ACCAGCTAGG CATTCGTCCA 
CCAACCCGGA CTGCCCCCTC CACAAATAGC TCAGCGCCTG CAGTGGTGGG GAATCCTGGT 
GTGACTGAAG TACTGGCACA GCAGAGAGCT GAGCAGCAGC GACGAGAACT AGCACAGAAT 
GCCAGCTCAG ACACCCCTAT GGACCCTGTG ACCTTCATCC AGACTCTGCC CTCAGACCTG 
CGCCGTAGTG TCCTAGAGGA TATGGAGGAC AGTGTGTTAG CTGTGATGCC ACCTGACATT 
GCAGCTGAGG CTCAAGCCCT GAGACGAGAG CAAGAAGCCC GGCAGCGACA GCTCATGCAT 
GAGCGTCTGT TTGGGCACAG TAGCACCTCC GCACTCTCTG CTATTCTCCG AAGCCCGGCT 
TTCACCAGTC GCTTAAGTGG CAACCGTGGG GTCCAGTATA CTCGCCTTGC TGTGCAGAGA 
GGTGGCACCT TCCAGATGGG GGGTAGCAGC AGCCATAACA GGCCTTCTGG CAGTAATGTA 
GATACTCTCC TCCGCCTCCG AGGACGGCTC CTTCTGGACC ACGAAGCCCT TTCTTGTCTC 
TTGGTCCTAC TTTTTGTGGA TGAGCCAAAG CTCAATACTA GCCGTCTACA CCGAGTACTG 
AGAAATCTCT GCTACCATGC CCAGACCCGC CACTGGGTCA TCCGCAGTCT GCTCTCCATC 
TTGCAGCGCA GCAGTGAGAG TGAGCTATGC ATTGAAACAC CCAAACTCAC TACAAGTGAG 
GAAAAGGGCA AAAAGTCGAG CAAGAGCTGT GGGTCAAGTA GCCATGAGAA CCGTCCCCTG 
GACCTGCTAC ACAAGATGGA GTCAAAGAGC TCCAACCAGC TTTCCTGGCT CTCAGTATCC 
ATGGATGCAG CCCTAGGCTG CAGGACTAAT ATATTTCAGA TCCAGCGTTC AGGGGGGCGT 
AAACATACCG AGAAGCATGC AAGCGGTGGC TCCACCGTCC ACATCCATCC CCAAGCTGCT 
CCTGTTGTCT GCAGACACGT TTTGGATACA CTCATTCAAT TGGCCAAGGT ATTTCCCAGC 
CACTTCACAC AGCAGCGGAC CAAAGAAACA AACTGTGAGA GTGATCGGGA AAGGGGCAAT 
AAGGCCTGTA GCCCATGCTC CTCACAGTCC TCCAGCAGTG GCATTTGCAC AGACTTCTGG 
GACTTATTGG TAAAACTGGA CAACATGAAT GTCAGCCGGA AAGGCAAGAA CTCCGTGAAG 
TCAGTGCCAG TGAGCGCTGG CGGTGAGGGG GAAACCTCTC CATACAGCCT CGAGGCCTCT 
CCACTGGGGC AGCTCATGAA CATGTTGTCA CACCCAGTCA TCCGCCGGAG CTCTCTCTTA 
ACTGAGAAAC TCCTCAGACT CCTTTCTCTC ATCTCAATTG CTCTCCCAGA AAACAAGGTG 
TCAGAAGCAC AGGCTAATTC TGGCAGCGGT GCTTCCTCCA CCACCACTGC CACCTCAACC 
ACATCTACCA CCACCACCAC TGCCGCCTCC ACCACGCCCA CACCCCCTAC TGCACCCACC 
CCTGTCACTT CTGCTCCAGC CCTGGTTGCT GCCACGGCTA TTTCCACCAT TGTCGTAGCT 
GCTTCGACCA CAGTGACTAC CCCCACGACT GCTACCACTA CTGTTTCAAT TTCTCCCACT 
ACTAAGGGCA GCAAATCTCC AGCGAAGGTG AGTGATGGGG GCAGCAGCAG TACAGACTTT 
AAGATGGTGT CCTCTGGCCT CACTGAAAAC CAGCTACAGC TCTCTGTAGA GGTGTTGACA 
TCCCACTCTT GTTCTGAGGA AGGCTTAGAG GATGCAGCCA ACGTACTACT GCAGCTCTCC 
CGGGGGGACT CTGGGACCCG GGACACTGTT CTCAAGCTGC TACTGAATGG AGCCCGCCAT 
CTGGGTTATA CCCTTTGTAA ACAAATAGGT ACCCTGCTGG CCGAGCTGCG GGAATACAAC 
CTCGAGCAGC AGCGGCGAGC CCAATGTGAA ACCCTCTCTC CTGATGGCCT GCCTGAGGAG 
CAGCCACAGA CCACCAAGCT GAAGGGCAAA ATGCAGAGCA GGTTTGACAT GGCTGAGAAT 
GTGGTAATTG TGGCATCTCA GAAGCGACCT TTGGGTGGCC GGGAGCTCCA GCTGCCTTCT 
ATGTCCATGT TGACATCCAA GACATCTACC CAGAAGTTCT TCTTGAGGGT ACTACAGGTC 
ATCATCCAGC TCCGGGACGA CACGCGCCGG GCTAACAAGA AAGCCAAGCA GACAGGCAGG 
CTAGGTTCCT CCGGTTTAGG CTCAGCTAGC AGCATCCAGG CAGCTGTTCG GCAGCTGGAG 
GCTGAGGCTG ATGCCATTAT ACAAATGGTA CGTGAGGGTC AAAGGGCGCG GAGACAGCAA 
CAAGCAGCAA CGTCGGAGTC TAGCCAGTCA GAGGCGTCTG TCCGGAGGGA GGAATCACCC 
ATGGATGTGG ACCAGCCATC TCCCAGTGCT CAAGATACTC AATCCATTGC CTCCGATGGA 
ACCCCACAGG GGGAGAAGGA AAAGGAAGAA AGACCACCTG AGTTACCCCT GCTCAGCGAG 
CAGCTGAGTT TGGACGAGCT GTGGGACATG CTTGGGGAGT GTCTAAAGGA ACTAGAGGAA 
TCCCATGACC AGCATGCGGT GCTAGTGCTA CAGCCTGCTG TCGAGGCCTT CTTTCTGGTC 
CATGCCACAG AGCGGGAGAG CAAGCCTCCT GTCCGAGACA CCCGTGAGAG CCAGCTGGCA 
CACATCAAGG ACGAGCCTCC TCCACTCTCC CCTGCCCCCT TAACCCCAGC CACGCCTTCC 
TCCCTTGACC CATTCTTCTC CCGGGAGCCC TCATCTATGC ACATCTCCTC AAGCCTGCCC 
CCTGACACAC AGAAGTTCCT TCGCTTTGCA GAGACTCACC GCACTGTGTT AAACCAGATC 
CTACGGCAGT CCACGACCCA CCTTGCTGAT GGGCCTTTTG CTGTCCTGGT AGACTACATT 
CGTGTCCTCG ACTTTGATGT CAAGCGCAAA TATTTCCGCC AAGAGCTGGA GCGTTTAGAT 
GAGGGGCTCC GGAAAGAAGA CATGGCTGTG CATGTCCGTC GTGACCATGT GTTTGAAGAC 
TCCTATCGTG AGCTGCATCG CAAATCCCCC GAAGAAATGA AGAATCGATT GTATATAGTA 
TTTGAAGGAG AAGAAGGGCA GGATGCTGGT GGGCTCCTGC GGGAGTGGTA TATGATCATC 
TCTCGAGAGA TGTTTAACCC TATGTATGCC TTGTTCCGTA CCTCACCTGG TGATCGAGTC 
ACCTACACCA TCAATCCATC TTCCCACTGC AACCCCAACC ACCTCAGCTA CTTCAAGTTT 
GTCGGACGCA TTGTGGCCAA AGCTGTATAT GACAACCGTC TTCTGGAGTG CTACTTTACT 
CGATCCTTTT ACAAACACAT CTTGGGCAAG TCAGTCAGAT ATACAGATAT GGAGAGTGAA 
GATTACCACT TCTACCAAGG TCTGGTTTAT CTGCTGGAAA ATGATGTCTC CACACTAGGC 
TATGACCTCA CCTTCAGCAC TGAGGTCCAA GAGTTTGGAG TTTGTGAAGT TCGTGACCTC 
AAACCCAATG GGGCCAACAT CTTGGTAACA GAGGAGAATA AGAAGGAGTA TGTACACCTG 
GTATGCCAGA TGAGAATGAC AGGAGCCATC CGCAAGCAGT TGGCGGCTTT CTTAGAAGGC 
TTCTATGAGA TCATTCCAAA GCGCCTCATT TCCATCTTCA CTGAGCAGGA GTTAGAGCTG 
CTTATATCAG GACTGCCCAC CATTGACATC GATGATCTGA AATCCAACAC TGAATACCAC 
AAGTACCAGT CCAACTCTAT TCAGATCCAG TGGTTCTGGA GAGCATTGCG TTCTTTCGAT 
CAAGCTGACC GTGCCAAGTT CCTCCAGTTT GTCACAGGTA CTTCCAAGGT ACCCCTGCAA 
GGCTTTGCTG CCCTCGAAGG CATGAATGGC ATTCAGAAGT TTCAGATCCA TCGAGATGAC 
AGGTCCACAG ATCGCCTGCC TTCAGCTCAC ACATGTTTTA ATCAGCTGGA TCTGCCTGCC 
TATGAGAGCT TTGAGAAGCT CCGCCACATG CTACTGTTGG CTATCCAGGA GTGCTCTGAA 
GGCTTTGGGC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884684.1
CDS	3018..16475
Translation	MDPFISRREQKCMEIGTPRKNGVLVLRSSEAVYHLTTPFQIVCLHLPLSKSKPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029195.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029195.1

ORF Insert Sequence:

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GTTGCAGATG TGGCTATGGA AAGCAGTCCA GGCTCATCCA TCTCTATGGA GCACAGGCTG 
GATGTTGAAT TAAGGGCATC AGGTTCCAGC AGCAGCACTA ACATCTCTTC TGGCCCCAGC 
CCTGGTCCCA GTCCCGGCCC CGGCACCGGC CCTGGCCCCG GCCCCGGCCC CGGCCCCGGC 
CCTGGCCCCG GCCCCGGCCC CGGTCCTGGT CCCGGCCCTG GCCCCGGCCC TGGCCCTGGC 
CCCCGTCCTG GAGTCCAGTG TATTCCACAA CGAGCAGCAC TTCTGAAATC CATGTTGAAT 
TTCCTCAAGA AGGCCATCCA AGACCCTGCT TTCTCAGATG GCATACGACA TGTGATGGAT 
GGTTCTCTGC CTACCTCCCT GAAACACATC ATCAGCAATG CAGAATACTA TGGCCCATCA 
CTCTTCCTCC TAGCTACTGA AGTGGTGACT GTGTTTGTAT TTCAAGAACC ATCACTGCTC 
TCCTCACTCC AGGACAATGG ATTGACAGAT GTCATGCTGC ATGCACTGCT TATCAAAGAT 
GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC CTCCCAAATG TATTCAGTGC ACTCTGTTTG 
AATGCCCGAG GTCTTCAGTC TTTTGTTCAG TGTCAGCCTT TTGAACGCCT CTTCAAAGTT 
CTTCTGTCTC CAGATTACCT CCCAGCCATG CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG 
GATACTGCAT CCAACCTGGG GAGTGCTGTC GATGAGCTCA TGAGACATCA GCCCACCCTT 
AAAACAGATG CAACGACTGC CATCATCAAG TTACTTGAAG AAATCTGTAA TCTTGGAAGG 
GACCCCAAAT ACATCTGTCA GAAGCCATCA ATCCAGAAGG CAGATGGCAC TGCCACTGCT 
CCTCCCCCAA GGTCTAATCA TGCCGCAGAA GAAGCCTCTA GTGAGGATGA GGAGGAAGAG 
GAAGTACAGG CCATGCAGAG CTTTAATTCT ACCCAGCAAA ATGAAACTGA GCCTAATCAG 
CAGGTTGTTG GTACAGAGGA ACGTATTCCT ATTCCCCTCA TGGATTACAT CCTTAATGTG 
ATGAAATTTG TGGAATCTAT TCTGAGCAAC AATACAACAG ATGACCACTG CCAGGAATTT 
GTGAATCAGA AAGGACTGTT GCCTTTGGTT ACCATTTTGG GTCTTCCCAA TCTGCCCATT 
GACTTTCCCA CATCTGCTGC CTGTCAGGCT GTTGCAGGTG TCTGCAAATC CATATTGACA 
CTGTCACATG AACCCAAAGT CCTTCAAGAG GGTCTCCTTC AGTTGGACTC CATCCTCTCC 
TCCCTGGAGC CCTTACACCG CCCCATTGAA TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA 
CTGGCTTGCG CAGGCAATGT TGCTGATGCT ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG 
CATGCACTCA CTGCTGCCCA TGCCTACATC ATGATGTTTG TTCATACTTG CAGAGTTGGA 
CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC CAGTGGGGCT CTCAATTGGG TCTGAGTGTT 
TTGAGCAAGC TGAGCCAGTT ATACTGTTCC CTGGTGTGGG AAAGCACTGT CCTCCTCTCT 
CTGTGTACCC CAAACAGCCT ACCATCTGGG TGTGAATTTG GCCAGGCAGA TATGCAGAAA 
CTGGTTCCAA AGGATGAGAA GGCAGGTACG ACCCAGGGCG GAAAAAGATC AGATGGGGAA 
CAGGATGGAG CAGCTGGAAG TATGGATGCT TCTACCCAGG GCTTATTAGA AGGCATTGGG 
CTAGATGGTG ACACATTGGC TCCCATGGAG ACAGATGAAC CTACTGCTTC AGACTCTAAG 
GGCAAATCTA AAATCACACC AGCAATGGCT GCCAGAATTA AGCAAATCAA GCCTTTGTTA 
TCAGCTTCCT CCAGATTAGG CCGAGCACTT GCTGAGCTAT TTGGACTTCT TGTTAAACTT 
TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG AGCCATCATG CTGCCAGCAC CACTACAGCA 
CCGACACCTG CCGCGCGATC AACAGCCTCA GCTCTCACTA AGCTCTTGAC TAAGGGGTTA 
TCTTGGCAGC CCCCACCATA TACACCTACT CCCCGATTCA GGCTGACATT CTTCATCTGT 
TCAGTTGGTT TCACATCCCC AATGCTGTTT GATGAGAGGA AGTATCCCTA CCACCTCATG 
CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC AATGCTCTTT TTGAAACTTT CAACTGGGCT 
CTGTCCATGG GAGGTAAAGT TCCTGTTTCT GAGGGATTGG AACACTCAGA CTTGCCTGAT 
GGCACAGGAG AATTCCTAGA TGCCTGGCTT ATGCTGGTGG AGAAGATGGT GAATCCCACC 
ACGGTGCTTG AATCTCCACA TTCGCTGCCT GCCAAATTGC CTGGAGGTGT CCAGAACTTT 
CCCCAGTTCA GTGCACTGCG CTTCCTTGTG GTAACTCAGA AAGCAGCCTT TACTTGCATC 
AAAAACTTAT GGAACCGGAA ACCCCTGAAG GTATATGGTG GACGAATGGC TGAATCGATG 
CTGGCCATTC TATGCCACAT CCTCCGAGGA GAACCTGTGA TTCGAGAGAG ACTAAGCAAG 
GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG 
GAACCTCAAG TCAACCAGCA ACAACTGCAA CAGCTCATGG ACATGGGCTT CACAAGGGAA 
CATGCAATGG AGGCACTGTT GAACACCAGC ACCATGGAGC AGGCCACAGA GTACCTTTTA 
ACCCACCCTC CTCCAATCAT GGGAGGAGTT GTTCGGGATC TCAGCATGTC TGAAGAGGAC 
CAGATGATGA GAGCAATTGC TATGTCTCTG GGACAGGATA TTCCAATGGA TCAAAGGGCA 
GAGTCACCTG AGGAAGTTGC TTGCCGGAAG GAGGAAGAGG AACGGAAAGC TCGGGAAAAG 
CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG AAGTTCCAGG ATGCTGACCC GTTGGAACAA 
GATGAGCTCC ACACTTTCAC AGATACTATG TTGCCAGGCT GCTTCCACCT TCTTGATGAG 
CTGCCAGACA CAGTATACCG TGTGTGTGAC CTGATCATGA CAGCAATCAA ACGTAATGGA 
GCAGATTATC GTGACATGAT TCTGAAGCAA GTAGTCAATC AGGTGTGGGA AGCTGCTGAT 
GTATTGATCA AAGCTGCTCT TCCCCTGACA ACAAGTGACA CAAAAACCGT GTCAGAGTGG 
ATAAGTCAGA TGGCCACACT GCCCCAGGCC TCCAATTTGG CTACTAGAAT CTTGCTTTTA 
ACGCTACTTT TTGAGGAGTT GAAGCTACCT TGTGCTTGGG TGGTTGAATC AAGTGGCATC 
CTTAATGTCC TAATCAAACT CTTGGAAGTG GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG 
CAGAAGGAAG TCCAGACCCC AAAGTGGATC ACACCAGTGT TGCTCCTGAT TGATTTCTAT 
GAAAAGACAG CCATCTCCTC AAAAAGGAGA GCCCAGATGA CTAAGTACCT GCAATCCAAC 
AGCAACAACT GGCGCTGGTT TGATGATCGC TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC 
AACAATAGCA CTATTGATTC TGCCTGGAAA TCTGGAGAGA CAAGCGTGCG ATTCACTGCA 
GGCCGAAGAA GATACACGGT CCAATTCACT ACAATGGTGC AGGTTAATGA GGAAACAGGG 
AACCGACGCC CTGTGATGCT GACTCTCCTC AGGGTACCTC GGCTGAATAA AAATTCAAAA 
AACAGCAATG GACAGGAACT AGAGAAGACG CTGGAAGAAA GCAAAGAAAT GGATATCAAA 
CGTAAAGAAA ATAAAGGCAA TGATACCCCT TTGGCCCTAG AGAGTACAAA CACTGAAAAG 
GAGACAAGCC TGGAGGAAAC AAAAATCGGG GAGATCCTGA TCCAGGGCTT GACAGAAGAT 
ATGGTGACTG TTTTAATCCG GGCCTGCGTG AGCATGCTGG GAGTCCCTGT GGACCCAGAT 
ACTTTGCATG CCACCCTTCG TCTCTGTCTG AGGCTCACCC GGGACCACAA ATATGCCATG 
ATGTTTGCAG AACTGAAGAG TACCCGCATG ATCTTGAATT TGACCCAGAG CTCAGGCTTC 
AATGGGTTTA CTCCCCTGGT CACCCTTCTC TTAAGACACA TCATTGAGGA CCCCTGTACC 
CTTCGTCATA CCATGGAAAA GGTTGTTCGC TCAGCAGCTA CAAGTGGAGC TGGTAGCACT 
ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC TCTCGGGAGA TCAACTACAT CCTTCGTGTC 
CTTGGGCCAG CCGCATGCCG CAATCCAGAC ATATTCACAG AAGTGGCCAA CTGCTGTATC 
CGCATCGCCC TTCCTGCCCC TCGAGGCTCA GGAACTGCTT CAGATGATGA ATTTGAGAAT 
CTTAGAATTA AAGGCCCTAA TGCTGTACAG CTGGTGAAGA CCACCCCTTT GAAGCCCTCA 
CCTCTGCCTG TCATCCCTGA TACTATCAAG GAAGTGATCT ATGATATGCT GAATGCTCTG 
GCTGCATACC ATGCTCCAGA GGAAGCAGAT AAATCTGATC CTAAACCTGG GGTTATGACC 
CAAGAGGTTG GCCAGCTCCT GCAAGACATG GGTGATGATG TATACCAGCA GTACCGGTCA 
CTTACGCGTC AGAGCAGTGA CTTTGATACG CAGTCAGGTT TTTCCATTAA TAGTCAGGTC 
TTTGCTGCAG ATGGTGCCTC CACTGAGACT TCCGCATCTG GGACCTCCCA AGGAGAGGCT 
TCAACTCCAG AGGAGTCTCG AGATGGGAAG AAAGATAAAG AAGGGGACCG GGCCTCTGAG 
GAAGGCAAAC AGAAAGGCAA GGGCAGCAAA CCTTTAATGC CTACCTCCAC TATCCTTCGT 
CTTCTGGCAG AGTTGGTGAG GTCCTATGTT GGTATTGCTA CCCTGATTGC CAACTACAGC 
TACACTGTGG GCCAGTCTGA ACTGATCAAA GAGGACTGCA GTGTGCTAGC TTTTGTTCTG 
GACCACCTGC TCCCACATAC CCAGAATGCA GAAGACAAGG ACACCCCTGC CTTGGCCCGC 
CTGTTCCTCG CAAGCCTGGC TGCTGCAGGG AGTGGCACAG ATGCCCAGGT GGCCCTAGTG 
AATGAAGTAA AAGCAGCCCT TGGACGGGCA CTGGCTATGG CTGAGAGTAC AGAGAAACAT 
GCCAGGCTTC AGGCAGTGAT GTGTATCATC AGTACTATCA TGGAGTCCTG CCCCTCCACC 
TCCAGCTTCT ACAGCAGTGC CACAGCGAAG ACCCAGCACA ATGGCATGAA CAACATCATT 
CGGCTTTTCC TGAAGAAGGG ACTGGTTAAT GACCTGGCCA GAGTACCTCA CAGCTTAGAC 
CTGTCCAGTC CCAACATGGC CAACACAGTC AATGCTGCTC TGAAGCCTTT GGAAACACTT 
TCCCGGATTG TGAACCAGCC CAGTAGCCTT TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC 
AAGTCTGAGC AGGATGCCCA AGGAGCCTCT CAAGATTCCA GTAGCAACCA GCAGGACCCA 
GGCGAGCCTG GGGAAGCAGA AGTGCAGGAG GAGGATCATG ATGTCACTCA GACAGAGGTG 
GCAGATGGGG ATATCATGGA TGGGGAGGCT GAAACCGACT CAGTGGTGAT TGCTGGGCAG 
CCTGAGGTGC TCAGTTCACA AGAGATGCAG GTTGAGAATG AGCTGGAGGA CCTGATAGAT 
GAGTTGCTTG AGAGGGATGG CGGATCTGGG AACAGTACAA TTATAGTGAG CAGAAGTGGA 
GAGGATGAAT CACAAGAGGA CGTGCTGATG GATGAAGCTC CTTCCAACCT CAGCCAAGCT 
TCCACCTTGC AGGCCAACCG AGAAGATTCC ATGAATATCC TGGACCCTGA GGATGAGGAG 
GAGCACACTC AGGAAGAGGA CAGCAGTGGC AGTAACGAGG ATGAGGATGA TAGTCAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGAGGAA GATGATCAGG AGGATGATGA AGGTGAAGAG 
GGAGATGAAG ACGATGACGA CGATGGCTCT GAGATGGAAT TGGATGAGGA TTATCCTGAT 
ATGAACGCTT CTCCCTTGGT CCGATTTGAG CGCTTTGACC GGGAGGATGA TCTCATCATT 
GAGTTTGACA ACATGTTCTC CAGTGCTACA GACATCCCCC CATCCCCAGG AAATATCCCT 
ACCACCCATC CACTGATGGT GCGCCATGCA GACCACAGTT CTCTGACACT GGGCAGTGGC 
TCTTCAACAA CTCGTCTCAC CCAGGGCATC GGGCGCAGTC AGAGGACCCT AAGGCAGCTG 
ACGGCCAATA CTGGCCACAC CATTCATGTT CACTACCCTG GGAATCGCCA GCCCAACCCT 
CCTCTTATAC TGCAGAGGTT GCTTGGTCCC TCAGCTGCTG CTGACATCCT TCAGCTGAGC 
AGCAGCCTTC CCCTACAAAG CCGGGGTCGG GCCCGCCTCC TGGTAGGCAA CGATGACGTC 
CACATCATCG CCCGTTCTGA TGATGAGCTG CTGGATGACT TTTTCCATGA TCAGAGCACA 
GCTACCAGCC AAGCAGGAAC CCTGTCCAGC ATCCCCACAG CCCTGACCCG CTGGACAGAA 
GAATGCAAAG TTCTCGATGC TGAGAGCATG CATGACTGTG TTTCAGTGGT TAAAGTGTCC 
ATTGTCAATC ACCTGGAATT CCTGAGGGAT GAGGAGCTGG AAGAAAGGCG AGAGAAGCGC 
AGGAAACAAC TGGCTGAGGA AGAAACAAAG ATAACTGATA AAGGCAAAGA AGATAAGGAG 
AACAGGGATC AGAGTGCACA GTGTACTGCA TCTAAGTCAA ATGACTCCAC TGAACAGAAT 
CTCTCAGATG GGACGCCTAT GCCTGACAGC TACCCAACAA CCCCATCTTC AACTGATGCA 
GCTACATCTG AGTCCAAGGA GACCCTTGGC ACTCTGCAAT CCTCACAACA GCAACCAACA 
CTCCCAACCC CACCAGCTTT GGGAGAGGTT CCTCAGGAGC TGCAGTCTCC AGCTGGAGAA 
GGGGGCAGCT CTACACAGCT ATTGATGCCT GTAGAGCCAG AGGAATTGGG TCCCACAAGG 
CCAAGTGGGG AAGCAGAAAC AACTCAGATG GAGTTATCCC CAGCTCCCAC TATAACCTCA 
CTTTCCCCAG AGAGAGCTGA GGATTCTGAT GCACTGACGG CTGTCAGCAG TCAGCTAGAA 
GGCTCTCCTA TGGATACAAG CAGCCTGGCT TCCTGTACCT TAGAGGAGGC TGTGGGTGAC 
ACTTCAGCAG CTGGCAGTTC TGAGCAGCCC AGAGCAGGCA GCTCCACTCC TGGGGATGCC 
CCACCAGCTG TGGCGGAAGT GCAAGGCAGG AGTGATGGGT CAGGGGAATC TGCCCAGCCA 
CCTGAGGACA GCTCCCCACC TGCATCCTCT GAGAGCTCTT CCACCAGAGA TTCTGCCGTG 
GCCATTTCTG GAGCAGATTC CCGAGGAATC CTAGAAGAGC CGTTGCCTTC AACAAGCAGT 
GAAGAAGAAG ATCCCCTTGC GGGTATCAGT CTCCCTGAAG GTGTGGACCC CTCTTTTCTG 
GCTGCCCTGC CTGATGACAT CCGTCGGGAA GTTCTACAGA ACCAGCTAGG CATTCGTCCA 
CCAACCCGGA CTGCCCCCTC CACAAATAGC TCAGCGCCTG CAGTGGTGGG GAATCCTGGT 
GTGACTGAAG TACTGGCACA GCAGAGAGCT GAGCAGCAGC GACGAGAACT AGCACAGAAT 
GCCAGCTCAG ACACCCCTAT GGACCCTGTG ACCTTCATCC AGACTCTGCC CTCAGACCTG 
CGCCGTAGTG TCCTAGAGGA TATGGAGGAC AGTGTGTTAG CTGTGATGCC ACCTGACATT 
GCAGCTGAGG CTCAAGCCCT GAGACGAGAG CAAGAAGCCC GGCAGCGACA GCTCATGCAT 
GAGCGTCTGT TTGGGCACAG TAGCACCTCC GCACTCTCTG CTATTCTCCG AAGCCCGGCT 
TTCACCAGTC GCTTAAGTGG CAACCGTGGG GTCCAGTATA CTCGCCTTGC TGTGCAGAGA 
GGTGGCACCT TCCAGATGGG GGGTAGCAGC AGCCATAACA GGCCTTCTGG CAGTAATGTA 
GATACTCTCC TCCGCCTCCG AGGACGGCTC CTTCTGGACC ACGAAGCCCT TTCTTGTCTC 
TTGGTCCTAC TTTTTGTGGA TGAGCCAAAG CTCAATACTA GCCGTCTACA CCGAGTACTG 
AGAAATCTCT GCTACCATGC CCAGACCCGC CACTGGGTCA TCCGCAGTCT GCTCTCCATC 
TTGCAGCGCA GCAGTGAGAG TGAGCTATGC ATTGAAACAC CCAAACTCAC TACAAGTGAG 
GAAAAGGGCA AAAAGTCGAG CAAGAGCTGT GGGTCAAGTA GCCATGAGAA CCGTCCCCTG 
GACCTGCTAC ACAAGATGGA GTCAAAGAGC TCCAACCAGC TTTCCTGGCT CTCAGTATCC 
ATGGATGCAG CCCTAGGCTG CAGGACTAAT ATATTTCAGA TCCAGCGTTC AGGGGGGCGT 
AAACATACCG AGAAGCATGC AAGCGGTGGC TCCACCGTCC ACATCCATCC CCAAGCTGCT 
CCTGTTGTCT GCAGACACGT TTTGGATACA CTCATTCAAT TGGCCAAGGT ATTTCCCAGC 
CACTTCACAC AGCAGCGGAC CAAAGAAACA AACTGTGAGA GTGATCGGGA AAGGGGCAAT 
AAGGCCTGTA GCCCATGCTC CTCACAGTCC TCCAGCAGTG GCATTTGCAC AGACTTCTGG 
GACTTATTGG TAAAACTGGA CAACATGAAT GTCAGCCGGA AAGGCAAGAA CTCCGTGAAG 
TCAGTGCCAG TGAGCGCTGG CGGTGAGGGG GAAACCTCTC CATACAGCCT CGAGGCCTCT 
CCACTGGGGC AGCTCATGAA CATGTTGTCA CACCCAGTCA TCCGCCGGAG CTCTCTCTTA 
ACTGAGAAAC TCCTCAGACT CCTTTCTCTC ATCTCAATTG CTCTCCCAGA AAACAAGGTG 
TCAGAAGCAC AGGCTAATTC TGGCAGCGGT GCTTCCTCCA CCACCACTGC CACCTCAACC 
ACATCTACCA CCACCACCAC TGCCGCCTCC ACCACGCCCA CACCCCCTAC TGCACCCACC 
CCTGTCACTT CTGCTCCAGC CCTGGTTGCT GCCACGGCTA TTTCCACCAT TGTCGTAGCT 
GCTTCGACCA CAGTGACTAC CCCCACGACT GCTACCACTA CTGTTTCAAT TTCTCCCACT 
ACTAAGGGCA GCAAATCTCC AGCGAAGGTG AGTGATGGGG GCAGCAGCAG TACAGACTTT 
AAGATGGTGT CCTCTGGCCT CACTGAAAAC CAGCTACAGC TCTCTGTAGA GGTGTTGACA 
TCCCACTCTT GTTCTGAGGA AGGCTTAGAG GATGCAGCCA ACGTACTACT GCAGCTCTCC 
CGGGGGGACT CTGGGACCCG GGACACTGTT CTCAAGCTGC TACTGAATGG AGCCCGCCAT 
CTGGGTTATA CCCTTTGTAA ACAAATAGGT ACCCTGCTGG CCGAGCTGCG GGAATACAAC 
CTCGAGCAGC AGCGGCGAGC CCAATGTGAA ACCCTCTCTC CTGATGGCCT GCCTGAGGAG 
CAGCCACAGA CCACCAAGCT GAAGGGCAAA ATGCAGAGCA GGTTTGACAT GGCTGAGAAT 
GTGGTAATTG TGGCATCTCA GAAGCGACCT TTGGGTGGCC GGGAGCTCCA GCTGCCTTCT 
ATGTCCATGT TGACATCCAA GACATCTACC CAGAAGTTCT TCTTGAGGGT ACTACAGGTC 
ATCATCCAGC TCCGGGACGA CACGCGCCGG GCTAACAAGA AAGCCAAGCA GACAGGCAGG 
CTAGGTTCCT CCGGTTTAGG CTCAGCTAGC AGCATCCAGG CAGCTGTTCG GCAGCTGGAG 
GCTGAGGCTG ATGCCATTAT ACAAATGGTA CGTGAGGGTC AAAGGGCGCG GAGACAGCAA 
CAAGCAGCAA CGTCGGAGTC TAGCCAGTCA GAGGCGTCTG TCCGGAGGGA GGAATCACCC 
ATGGATGTGG ACCAGCCATC TCCCAGTGCT CAAGATACTC AATCCATTGC CTCCGATGGA 
ACCCCACAGG GGGAGAAGGA AAAGGAAGAA AGACCACCTG AGTTACCCCT GCTCAGCGAG 
CAGCTGAGTT TGGACGAGCT GTGGGACATG CTTGGGGAGT GTCTAAAGGA ACTAGAGGAA 
TCCCATGACC AGCATGCGGT GCTAGTGCTA CAGCCTGCTG TCGAGGCCTT CTTTCTGGTC 
CATGCCACAG AGCGGGAGAG CAAGCCTCCT GTCCGAGACA CCCGTGAGAG CCAGCTGGCA 
CACATCAAGG ACGAGCCTCC TCCACTCTCC CCTGCCCCCT TAACCCCAGC CACGCCTTCC 
TCCCTTGACC CATTCTTCTC CCGGGAGCCC TCATCTATGC ACATCTCCTC AAGCCTGCCC 
CCTGACACAC AGAAGTTCCT TCGCTTTGCA GAGACTCACC GCACTGTGTT AAACCAGATC 
CTACGGCAGT CCACGACCCA CCTTGCTGAT GGGCCTTTTG CTGTCCTGGT AGACTACATT 
CGTGTCCTCG ACTTTGATGT CAAGCGCAAA TATTTCCGCC AAGAGCTGGA GCGTTTAGAT 
GAGGGGCTCC GGAAAGAAGA CATGGCTGTG CATGTCCGTC GTGACCATGT GTTTGAAGAC 
TCCTATCGTG AGCTGCATCG CAAATCCCCC GAAGAAATGA AGAATCGATT GTATATAGTA 
TTTGAAGGAG AAGAAGGGCA GGATGCTGGT GGGCTCCTGC GGGAGTGGTA TATGATCATC 
TCTCGAGAGA TGTTTAACCC TATGTATGCC TTGTTCCGTA CCTCACCTGG TGATCGAGTC 
ACCTACACCA TCAATCCATC TTCCCACTGC AACCCCAACC ACCTCAGCTA CTTCAAGTTT 
GTCGGACGCA TTGTGGCCAA AGCTGTATAT GACAACCGTC TTCTGGAGTG CTACTTTACT 
CGATCCTTTT ACAAACACAT CTTGGGCAAG TCAGTCAGAT ATACAGATAT GGAGAGTGAA 
GATTACCACT TCTACCAAGG TCTGGTTTAT CTGCTGGAAA ATGATGTCTC CACACTAGGC 
TATGACCTCA CCTTCAGCAC TGAGGTCCAA GAGTTTGGAG TTTGTGAAGT TCGTGACCTC 
AAACCCAATG GGGCCAACAT CTTGGTAACA GAGGAGAATA AGAAGGAGTA TGTACACCTG 
GTATGCCAGA TGAGAATGAC AGGAGCCATC CGCAAGCAGT TGGCGGCTTT CTTAGAAGGC 
TTCTATGAGA TCATTCCAAA GCGCCTCATT TCCATCTTCA CTGAGCAGGA GTTAGAGCTG 
CTTATATCAG GACTGCCCAC CATTGACATC GATGATCTGA AATCCAACAC TGAATACCAC 
AAGTACCAGT CCAACTCTAT TCAGATCCAG TGGTTCTGGA GAGCATTGCG TTCTTTCGAT 
CAAGCTGACC GTGCCAAGTT CCTCCAGTTT GTCACAGGTA CTTCCAAGGT ACCCCTGCAA 
GGCTTTGCTG CCCTCGAAGG CATGAATGGC ATTCAGAAGT TTCAGATCCA TCGAGATGAC 
AGGTCCACAG ATCGCCTGCC TTCAGCTCAC ACATGTTTTA ATCAGCTGGA TCTGCCTGCC 
TATGAGAGCT TTGAGAAGCT CCGCCACATG CTACTGTTGG CTATCCAGGA GTGCTCTGAA 
GGCTTTGGGC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029196.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884685.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X6, mRNA.

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CloneID	OHu99117
Clone ID Related Accession (Same CDS sequence)	XM_017029196.1
Accession Version	XM_017029196.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GTTGCAGATG TGGCTATGGA AAGCAGTCCA GGCTCATCCA TCTCTATGGA GCACAGGCTG 
GATGTTGAAT TAAGGGCATC AGGTTCCAGC AGCAGCACTA ACATCTCTTC TGGCCCCAGC 
CCTGGTCCCA GTCCCGGCCC CGGCACCGGC CCTGGCCCCG GCCCCGGCCC CGGCCCCGGC 
CCTGGCCCCG GCCCCGGCCC CGGTCCTGGT CCCGGCCCTG GCCCCGGCCC TGGCCCTGGC 
CCCCGTCCTG GAGTCCAGTG TATTCCACAA CGAGCAGCAC TTCTGAAATC CATGTTGAAT 
TTCCTCAAGA AGGCCATCCA AGACCCTGCT TTCTCAGATG GCATACGACA TGTGATGGAT 
GGTTCTCTGC CTACCTCCCT GAAACACATC ATCAGCAATG CAGAATACTA TGGCCCATCA 
CTCTTCCTCC TAGCTACTGA AGTGGTGACT GTGTTTGTAT TTCAAGAACC ATCACTGCTC 
TCCTCACTCC AGGACAATGG ATTGACAGAT GTCATGCTGC ATGCACTGCT TATCAAAGAT 
GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC CTCCCAAATG TATTCAGTGC ACTCTGTTTG 
AATGCCCGAG GTCTTCAGTC TTTTGTTCAG TGTCAGCCTT TTGAACGCCT CTTCAAAGTT 
CTTCTGTCTC CAGATTACCT CCCAGCCATG CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG 
GATACTGCAT CCAACCTGGG GAGTGCTGTC GATGAGCTCA TGAGACATCA GCCCACCCTT 
AAAACAGATG CAACGACTGC CATCATCAAG TTACTTGAAG AAATCTGTAA TCTTGGAAGG 
GACCCCAAAT ACATCTGTCA GAAGCCATCA ATCCAGAAGG CAGATGGCAC TGCCACTGCT 
CCTCCCCCAA GGTCTAATCA TGCCGCAGAA GAAGCCTCTA GTGAGGATGA GGAGGAAGAG 
GAAGTACAGG CCATGCAGAG CTTTAATTCT ACCCAGCAAA ATGAAACTGA GCCTAATCAG 
CAGGTTGTTG GTACAGAGGA ACGTATTCCT ATTCCCCTCA TGGATTACAT CCTTAATGTG 
ATGAAATTTG TGGAATCTAT TCTGAGCAAC AATACAACAG ATGACCACTG CCAGGAATTT 
GTGAATCAGA AAGGACTGTT GCCTTTGGTT ACCATTTTGG GTCTTCCCAA TCTGCCCATT 
GACTTTCCCA CATCTGCTGC CTGTCAGGCT GTTGCAGGTG TCTGCAAATC CATATTGACA 
CTGTCACATG AACCCAAAGT CCTTCAAGAG GGTCTCCTTC AGTTGGACTC CATCCTCTCC 
TCCCTGGAGC CCTTACACCG CCCCATTGAA TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA 
CTGGCTTGCG CAGGCAATGT TGCTGATGCT ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG 
CATGCACTCA CTGCTGCCCA TGCCTACATC ATGATGTTTG TTCATACTTG CAGAGTTGGA 
CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC CAGTGGGGCT CTCAATTGGG TCTGAGTGTT 
TTGAGCAAGC TGAGCCAGTT ATACTGTTCC CTGGTGTGGG AAAGCACTGT CCTCCTCTCT 
CTGTGTACCC CAAACAGCCT ACCATCTGGG TGTGAATTTG GCCAGGCAGA TATGCAGAAA 
CTGGTTCCAA AGGATGAGAA GGCAGGTACG ACCCAGGGCG GAAAAAGATC AGATGGGGAA 
CAGGATGGAG CAGCTGGAAG TATGGATGCT TCTACCCAGG GCTTATTAGA AGGCATTGGG 
CTAGATGGTG ACACATTGGC TCCCATGGAG ACAGATGAAC CTACTGCTTC AGACTCTAAG 
GGCAAATCTA AAATCACACC AGCAATGGCT GCCAGAATTA AGCAAATCAA GCCTTTGTTA 
TCAGCTTCCT CCAGATTAGG CCGAGCACTT GCTGAGCTAT TTGGACTTCT TGTTAAACTT 
TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG AGCCATCATG CTGCCAGCAC CACTACAGCA 
CCGACACCTG CCGCGCGATC AACAGCCTCA GCTCTCACTA AGCTCTTGAC TAAGGGGTTA 
TCTTGGCAGC CCCCACCATA TACACCTACT CCCCGATTCA GGCTGACATT CTTCATCTGT 
TCAGTTGGTT TCACATCCCC AATGCTGTTT GATGAGAGGA AGTATCCCTA CCACCTCATG 
CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC AATGCTCTTT TTGAAACTTT CAACTGGGCT 
CTGTCCATGG GAGGTAAAGT TCCTGTTTCT GAGGGATTGG AACACTCAGA CTTGCCTGAT 
GGCACAGGAG AATTCCTAGA TGCCTGGCTT ATGCTGGTGG AGAAGATGGT GAATCCCACC 
ACGGTGCTTG AATCTCCACA TTCGCTGCCT GCCAAATTGC CTGGAGGTGT CCAGAACTTT 
CCCCAGTTCA GTGCACTGCG CTTCCTTGTG GTAACTCAGA AAGCAGCCTT TACTTGCATC 
AAAAACTTAT GGAACCGGAA ACCCCTGAAG GTATATGGTG GACGAATGGC TGAATCGATG 
CTGGCCATTC TATGCCACAT CCTCCGAGGA GAACCTGTGA TTCGAGAGAG ACTAAGCAAG 
GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG 
GAACCTCAAG TCAACCAGCA ACAACTGCAA CAGCTCATGG ACATGGGCTT CACAAGGGAA 
CATGCAATGG AGGCACTGTT GAACACCAGC ACCATGGAGC AGGCCACAGA GTACCTTTTA 
ACCCACCCTC CTCCAATCAT GGGAGGAGTT GTTCGGGATC TCAGCATGTC TGAAGAGGAC 
CAGATGATGA GAGCAATTGC TATGTCTCTG GGACAGGATA TTCCAATGGA TCAAAGGGCA 
GAGTCACCTG AGGAAGTTGC TTGCCGGAAG GAGGAAGAGG AACGGAAAGC TCGGGAAAAG 
CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG AAGTTCCAGG ATGCTGACCC GTTGGAACAA 
GATGAGCTCC ACACTTTCAC AGATACTATG TTGCCAGGCT GCTTCCACCT TCTTGATGAG 
CTGCCAGACA CAGTATACCG TGTGTGTGAC CTGATCATGA CAGCAATCAA ACGTAATGGA 
GCAGATTATC GTGACATGAT TCTGAAGCAA GTAGTCAATC AGGTGTGGGA AGCTGCTGAT 
GTATTGATCA AAGCTGCTCT TCCCCTGACA ACAAGTGACA CAAAAACCGT GTCAGAGTGG 
ATAAGTCAGA TGGCCACACT GCCCCAGGCC TCCAATTTGG CTACTAGAAT CTTGCTTTTA 
ACGCTACTTT TTGAGGAGTT GAAGCTACCT TGTGCTTGGG TGGTTGAATC AAGTGGCATC 
CTTAATGTCC TAATCAAACT CTTGGAAGTG GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG 
CAGAAGGAAG TCCAGACCCC AAAGTGGATC ACACCAGTGT TGCTCCTGAT TGATTTCTAT 
GAAAAGACAG CCATCTCCTC AAAAAGGAGA GCCCAGATGA CTAAGTACCT GCAATCCAAC 
AGCAACAACT GGCGCTGGTT TGATGATCGC TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC 
AACAATAGCA CTATTGATTC TGCCTGGAAA TCTGGAGAGA CAAGCGTGCG ATTCACTGCA 
GGCCGAAGAA GATACACGGT CCAATTCACT ACAATGGTGC AGGTTAATGA GGAAACAGGG 
AACCGACGCC CTGTGATGCT GACTCTCCTC AGGGTACCTC GGCTGAATAA AAATTCAAAA 
AACAGCAATG GACAGGAACT AGAGAAGACG CTGGAAGAAA GCAAAGAAAT GGATATCAAA 
CGTAAAGAAA ATAAAGGCAA TGATACCCCT TTGGCCCTAG AGAGTACAAA CACTGAAAAG 
GAGACAAGCC TGGAGGAAAC AAAAATCGGG GAGATCCTGA TCCAGGGCTT GACAGAAGAT 
ATGGTGACTG TTTTAATCCG GGCCTGCGTG AGCATGCTGG GAGTCCCTGT GGACCCAGAT 
ACTTTGCATG CCACCCTTCG TCTCTGTCTG AGGCTCACCC GGGACCACAA ATATGCCATG 
ATGTTTGCAG AACTGAAGAG TACCCGCATG ATCTTGAATT TGACCCAGAG CTCAGGCTTC 
AATGGGTTTA CTCCCCTGGT CACCCTTCTC TTAAGACACA TCATTGAGGA CCCCTGTACC 
CTTCGTCATA CCATGGAAAA GGTTGTTCGC TCAGCAGCTA CAAGTGGAGC TGGTAGCACT 
ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC TCTCGGGAGA TCAACTACAT CCTTCGTGTC 
CTTGGGCCAG CCGCATGCCG CAATCCAGAC ATATTCACAG AAGTGGCCAA CTGCTGTATC 
CGCATCGCCC TTCCTGCCCC TCGAGGCTCA GGAACTGCTT CAGATGATGA ATTTGAGAAT 
CTTAGAATTA AAGGCCCTAA TGCTGTACAG CTGGTGAAGA CCACCCCTTT GAAGCCCTCA 
CCTCTGCCTG TCATCCCTGA TACTATCAAG GAAGTGATCT ATGATATGCT GAATGCTCTG 
GCTGCATACC ATGCTCCAGA GGAAGATAAA TCTGATCCTA AACCTGGGGT TATGACCCAA 
GAGGTTGGCC AGCTCCTGCA AGACATGGGT GATGATGTAT ACCAGCAGTA CCGGTCACTT 
ACGCGTCAGA GCAGTGACTT TGATACGCAG TCAGGTTTTT CCATTAATAG TCAGGTCTTT 
GCTGCAGATG GTGCCTCCAC TGAGACTTCC GCATCTGGGA CCTCCCAAGG AGAGGCTTCA 
ACTCCAGAGG AGTCTCGAGA TGGGAAGAAA GATAAAGAAG GGGACCGGGC CTCTGAGGAA 
GGCAAACAGA AAGGCAAGGG CAGCAAACCT TTAATGCCTA CCTCCACTAT CCTTCGTCTT 
CTGGCAGAGT TGGTGAGGTC CTATGTTGGT ATTGCTACCC TGATTGCCAA CTACAGCTAC 
ACTGTGGGCC AGTCTGAACT GATCAAAGAG GACTGCAGTG TGCTAGCTTT TGTTCTGGAC 
CACCTGCTCC CACATACCCA GAATGCAGAA GACAAGGACA CCCCTGCCTT GGCCCGCCTG 
TTCCTCGCAA GCCTGGCTGC TGCAGGGAGT GGCACAGATG CCCAGGTGGC CCTAGTGAAT 
GAAGTAAAAG CAGCCCTTGG ACGGGCACTG GCTATGGCTG AGAGTACAGA GAAACATGCC 
AGGCTTCAGG CAGTGATGTG TATCATCAGT ACTATCATGG AGTCCTGCCC CTCCACCTCC 
AGCTTCTACA GCAGTGCCAC AGCGAAGACC CAGCACAATG GCATGAACAA CATCATTCGG 
CTTTTCCTGA AGAAGGGACT GGTTAATGAC CTGGCCAGAG TACCTCACAG CTTAGACCTG 
TCCAGTCCCA ACATGGCCAA CACAGTCAAT GCTGCTCTGA AGCCTTTGGA AACACTTTCC 
CGGATTGTGA ACCAGCCCAG TAGCCTTTTT GGCAGCAAGA GTGCTTCTAG CAAGAACAAG 
TCTGAGCAGG ATGCCCAAGG AGCCTCTCAA GATTCCAGTA GCAACCAGCA GGACCCAGGC 
GAGCCTGGGG AAGCAGAAGT GCAGGAGGAG GATCATGATG TCACTCAGAC AGAGGTGGCA 
GATGGGGATA TCATGGATGG GGAGGCTGAA ACCGACTCAG TGGTGATTGC TGGGCAGCCT 
GAGGTGCTCA GTTCACAAGA GATGCAGGTT GAGAATGAGC TGGAGGACCT GATAGATGAG 
TTGCTTGAGA GGGATGGCGG ATCTGGGAAC AGTACAATTA TAGTGAGCAG AAGTGGAGAG 
GATGAATCAC AAGAGGACGT GCTGATGGAT GAAGCTCCTT CCAACCTCAG CCAAGCTTCC 
ACCTTGCAGG CCAACCGAGA AGATTCCATG AATATCCTGG ACCCTGAGGA TGAGGAGGAG 
CACACTCAGG AAGAGGACAG CAGTGGCAGT AACGAGGATG AGGATGATAG TCAGGATGAA 
GAGGAGGAGG AGGAGGAAGA TGAGGAAGAT GATCAGGAGG ATGATGAAGG TGAAGAGGGA 
GATGAAGACG ATGACGACGA TGGCTCTGAG ATGGAATTGG ATGAGGATTA TCCTGATATG 
AACGCTTCTC CCTTGGTCCG ATTTGAGCGC TTTGACCGGG AGGATGATCT CATCATTGAG 
TTTGACAACA TGTTCTCCAG TGCTACAGAC ATCCCCCCAT CCCCAGGAAA TATCCCTACC 
ACCCATCCAC TGATGGTGCG CCATGCAGAC CACAGTTCTC TGACACTGGG CAGTGGCTCT 
TCAACAACTC GTCTCACCCA GGGCATCGGG CGCAGTCAGA GGACCCTAAG GCAGCTGACG 
GCCAATACTG GCCACACCAT TCATGTTCAC TACCCTGGGA ATCGCCAGCC CAACCCTCCT 
CTTATACTGC AGAGGTTGCT TGGTCCCTCA GCTGCTGCTG ACATCCTTCA GCTGAGCAGC 
AGCCTTCCCC TACAAAGCCG GGGTCGGGCC CGCCTCCTGG TAGGCAACGA TGACGTCCAC 
ATCATCGCCC GTTCTGATGA TGAGCTGCTG GATGACTTTT TCCATGATCA GAGCACAGCT 
ACCAGCCAAG CAGGAACCCT GTCCAGCATC CCCACAGCCC TGACCCGCTG GACAGAAGAA 
TGCAAAGTTC TCGATGCTGA GAGCATGCAT GACTGTGTTT CAGTGGTTAA AGTGTCCATT 
GTCAATCACC TGGAATTCCT GAGGGATGAG GAGCTGGAAG AAAGGCGAGA GAAGCGCAGG 
AAACAACTGG CTGAGGAAGA AACAAAGATA ACTGATAAAG GCAAAGAAGA TAAGGAGAAC 
AGGGATCAGA GTGCACAGTG TACTGCATCT AAGTCAAATG ACTCCACTGA ACAGAATCTC 
TCAGATGGGA CGCCTATGCC TGACAGCTAC CCAACAACCC CATCTTCAAC TGATGCAGCT 
ACATCTGAGT CCAAGGAGAC CCTTGGCACT CTGCAATCCT CACAACAGCA ACCAACACTC 
CCAACCCCAC CAGCTTTGGG AGAGGTTCCT CAGGAGCTGC AGTCTCCAGC TGGAGAAGGG 
GGCAGCTCTA CACAGCTATT GATGCCTGTA GAGCCAGAGG AATTGGGTCC CACAAGGCCA 
AGTGGGGAAG CAGAAACAAC TCAGATGGAG TTATCCCCAG CTCCCACTAT AACCTCACTT 
TCCCCAGAGA GAGCTGAGGA TTCTGATGCA CTGACGGCTG TCAGCAGTCA GCTAGAAGGC 
TCTCCTATGG ATACAAGCAG CCTGGCTTCC TGTACCTTAG AGGAGGCTGT GGGTGACACT 
TCAGCAGCTG GCAGTTCTGA GCAGCCCAGA GCAGGCAGCT CCACTCCTGG GGATGCCCCA 
CCAGCTGTGG CGGAAGTGCA AGGCAGGAGT GATGGGTCAG GGGAATCTGC CCAGCCACCT 
GAGGACAGCT CCCCACCTGC ATCCTCTGAG AGCTCTTCCA CCAGAGATTC TGCCGTGGCC 
ATTTCTGGAG CAGATTCCCG AGGAATCCTA GAAGAGCCGT TGCCTTCAAC AAGCAGTGAA 
GAAGAAGATC CCCTTGCGGG TATCAGTCTC CCTGAAGGTG TGGACCCCTC TTTTCTGGCT 
GCCCTGCCTG ATGACATCCG TCGGGAAGTT CTACAGAACC AGCTAGGCAT TCGTCCACCA 
ACCCGGACTG CCCCCTCCAC AAATAGCTCA GCGCCTGCAG TGGTGGGGAA TCCTGGTGTG 
ACTGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884685.1
CDS	3018..16472
Translation	MDPFISRREQKCMEIGTPRKNGVLVLRSSEAVYHLTTPFQIVCLHLPLSKSKPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEDKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029196.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029196.1

ORF Insert Sequence:

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GTTGCAGATG TGGCTATGGA AAGCAGTCCA GGCTCATCCA TCTCTATGGA GCACAGGCTG 
GATGTTGAAT TAAGGGCATC AGGTTCCAGC AGCAGCACTA ACATCTCTTC TGGCCCCAGC 
CCTGGTCCCA GTCCCGGCCC CGGCACCGGC CCTGGCCCCG GCCCCGGCCC CGGCCCCGGC 
CCTGGCCCCG GCCCCGGCCC CGGTCCTGGT CCCGGCCCTG GCCCCGGCCC TGGCCCTGGC 
CCCCGTCCTG GAGTCCAGTG TATTCCACAA CGAGCAGCAC TTCTGAAATC CATGTTGAAT 
TTCCTCAAGA AGGCCATCCA AGACCCTGCT TTCTCAGATG GCATACGACA TGTGATGGAT 
GGTTCTCTGC CTACCTCCCT GAAACACATC ATCAGCAATG CAGAATACTA TGGCCCATCA 
CTCTTCCTCC TAGCTACTGA AGTGGTGACT GTGTTTGTAT TTCAAGAACC ATCACTGCTC 
TCCTCACTCC AGGACAATGG ATTGACAGAT GTCATGCTGC ATGCACTGCT TATCAAAGAT 
GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC CTCCCAAATG TATTCAGTGC ACTCTGTTTG 
AATGCCCGAG GTCTTCAGTC TTTTGTTCAG TGTCAGCCTT TTGAACGCCT CTTCAAAGTT 
CTTCTGTCTC CAGATTACCT CCCAGCCATG CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG 
GATACTGCAT CCAACCTGGG GAGTGCTGTC GATGAGCTCA TGAGACATCA GCCCACCCTT 
AAAACAGATG CAACGACTGC CATCATCAAG TTACTTGAAG AAATCTGTAA TCTTGGAAGG 
GACCCCAAAT ACATCTGTCA GAAGCCATCA ATCCAGAAGG CAGATGGCAC TGCCACTGCT 
CCTCCCCCAA GGTCTAATCA TGCCGCAGAA GAAGCCTCTA GTGAGGATGA GGAGGAAGAG 
GAAGTACAGG CCATGCAGAG CTTTAATTCT ACCCAGCAAA ATGAAACTGA GCCTAATCAG 
CAGGTTGTTG GTACAGAGGA ACGTATTCCT ATTCCCCTCA TGGATTACAT CCTTAATGTG 
ATGAAATTTG TGGAATCTAT TCTGAGCAAC AATACAACAG ATGACCACTG CCAGGAATTT 
GTGAATCAGA AAGGACTGTT GCCTTTGGTT ACCATTTTGG GTCTTCCCAA TCTGCCCATT 
GACTTTCCCA CATCTGCTGC CTGTCAGGCT GTTGCAGGTG TCTGCAAATC CATATTGACA 
CTGTCACATG AACCCAAAGT CCTTCAAGAG GGTCTCCTTC AGTTGGACTC CATCCTCTCC 
TCCCTGGAGC CCTTACACCG CCCCATTGAA TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA 
CTGGCTTGCG CAGGCAATGT TGCTGATGCT ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG 
CATGCACTCA CTGCTGCCCA TGCCTACATC ATGATGTTTG TTCATACTTG CAGAGTTGGA 
CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC CAGTGGGGCT CTCAATTGGG TCTGAGTGTT 
TTGAGCAAGC TGAGCCAGTT ATACTGTTCC CTGGTGTGGG AAAGCACTGT CCTCCTCTCT 
CTGTGTACCC CAAACAGCCT ACCATCTGGG TGTGAATTTG GCCAGGCAGA TATGCAGAAA 
CTGGTTCCAA AGGATGAGAA GGCAGGTACG ACCCAGGGCG GAAAAAGATC AGATGGGGAA 
CAGGATGGAG CAGCTGGAAG TATGGATGCT TCTACCCAGG GCTTATTAGA AGGCATTGGG 
CTAGATGGTG ACACATTGGC TCCCATGGAG ACAGATGAAC CTACTGCTTC AGACTCTAAG 
GGCAAATCTA AAATCACACC AGCAATGGCT GCCAGAATTA AGCAAATCAA GCCTTTGTTA 
TCAGCTTCCT CCAGATTAGG CCGAGCACTT GCTGAGCTAT TTGGACTTCT TGTTAAACTT 
TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG AGCCATCATG CTGCCAGCAC CACTACAGCA 
CCGACACCTG CCGCGCGATC AACAGCCTCA GCTCTCACTA AGCTCTTGAC TAAGGGGTTA 
TCTTGGCAGC CCCCACCATA TACACCTACT CCCCGATTCA GGCTGACATT CTTCATCTGT 
TCAGTTGGTT TCACATCCCC AATGCTGTTT GATGAGAGGA AGTATCCCTA CCACCTCATG 
CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC AATGCTCTTT TTGAAACTTT CAACTGGGCT 
CTGTCCATGG GAGGTAAAGT TCCTGTTTCT GAGGGATTGG AACACTCAGA CTTGCCTGAT 
GGCACAGGAG AATTCCTAGA TGCCTGGCTT ATGCTGGTGG AGAAGATGGT GAATCCCACC 
ACGGTGCTTG AATCTCCACA TTCGCTGCCT GCCAAATTGC CTGGAGGTGT CCAGAACTTT 
CCCCAGTTCA GTGCACTGCG CTTCCTTGTG GTAACTCAGA AAGCAGCCTT TACTTGCATC 
AAAAACTTAT GGAACCGGAA ACCCCTGAAG GTATATGGTG GACGAATGGC TGAATCGATG 
CTGGCCATTC TATGCCACAT CCTCCGAGGA GAACCTGTGA TTCGAGAGAG ACTAAGCAAG 
GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG 
GAACCTCAAG TCAACCAGCA ACAACTGCAA CAGCTCATGG ACATGGGCTT CACAAGGGAA 
CATGCAATGG AGGCACTGTT GAACACCAGC ACCATGGAGC AGGCCACAGA GTACCTTTTA 
ACCCACCCTC CTCCAATCAT GGGAGGAGTT GTTCGGGATC TCAGCATGTC TGAAGAGGAC 
CAGATGATGA GAGCAATTGC TATGTCTCTG GGACAGGATA TTCCAATGGA TCAAAGGGCA 
GAGTCACCTG AGGAAGTTGC TTGCCGGAAG GAGGAAGAGG AACGGAAAGC TCGGGAAAAG 
CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG AAGTTCCAGG ATGCTGACCC GTTGGAACAA 
GATGAGCTCC ACACTTTCAC AGATACTATG TTGCCAGGCT GCTTCCACCT TCTTGATGAG 
CTGCCAGACA CAGTATACCG TGTGTGTGAC CTGATCATGA CAGCAATCAA ACGTAATGGA 
GCAGATTATC GTGACATGAT TCTGAAGCAA GTAGTCAATC AGGTGTGGGA AGCTGCTGAT 
GTATTGATCA AAGCTGCTCT TCCCCTGACA ACAAGTGACA CAAAAACCGT GTCAGAGTGG 
ATAAGTCAGA TGGCCACACT GCCCCAGGCC TCCAATTTGG CTACTAGAAT CTTGCTTTTA 
ACGCTACTTT TTGAGGAGTT GAAGCTACCT TGTGCTTGGG TGGTTGAATC AAGTGGCATC 
CTTAATGTCC TAATCAAACT CTTGGAAGTG GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG 
CAGAAGGAAG TCCAGACCCC AAAGTGGATC ACACCAGTGT TGCTCCTGAT TGATTTCTAT 
GAAAAGACAG CCATCTCCTC AAAAAGGAGA GCCCAGATGA CTAAGTACCT GCAATCCAAC 
AGCAACAACT GGCGCTGGTT TGATGATCGC TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC 
AACAATAGCA CTATTGATTC TGCCTGGAAA TCTGGAGAGA CAAGCGTGCG ATTCACTGCA 
GGCCGAAGAA GATACACGGT CCAATTCACT ACAATGGTGC AGGTTAATGA GGAAACAGGG 
AACCGACGCC CTGTGATGCT GACTCTCCTC AGGGTACCTC GGCTGAATAA AAATTCAAAA 
AACAGCAATG GACAGGAACT AGAGAAGACG CTGGAAGAAA GCAAAGAAAT GGATATCAAA 
CGTAAAGAAA ATAAAGGCAA TGATACCCCT TTGGCCCTAG AGAGTACAAA CACTGAAAAG 
GAGACAAGCC TGGAGGAAAC AAAAATCGGG GAGATCCTGA TCCAGGGCTT GACAGAAGAT 
ATGGTGACTG TTTTAATCCG GGCCTGCGTG AGCATGCTGG GAGTCCCTGT GGACCCAGAT 
ACTTTGCATG CCACCCTTCG TCTCTGTCTG AGGCTCACCC GGGACCACAA ATATGCCATG 
ATGTTTGCAG AACTGAAGAG TACCCGCATG ATCTTGAATT TGACCCAGAG CTCAGGCTTC 
AATGGGTTTA CTCCCCTGGT CACCCTTCTC TTAAGACACA TCATTGAGGA CCCCTGTACC 
CTTCGTCATA CCATGGAAAA GGTTGTTCGC TCAGCAGCTA CAAGTGGAGC TGGTAGCACT 
ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC TCTCGGGAGA TCAACTACAT CCTTCGTGTC 
CTTGGGCCAG CCGCATGCCG CAATCCAGAC ATATTCACAG AAGTGGCCAA CTGCTGTATC 
CGCATCGCCC TTCCTGCCCC TCGAGGCTCA GGAACTGCTT CAGATGATGA ATTTGAGAAT 
CTTAGAATTA AAGGCCCTAA TGCTGTACAG CTGGTGAAGA CCACCCCTTT GAAGCCCTCA 
CCTCTGCCTG TCATCCCTGA TACTATCAAG GAAGTGATCT ATGATATGCT GAATGCTCTG 
GCTGCATACC ATGCTCCAGA GGAAGATAAA TCTGATCCTA AACCTGGGGT TATGACCCAA 
GAGGTTGGCC AGCTCCTGCA AGACATGGGT GATGATGTAT ACCAGCAGTA CCGGTCACTT 
ACGCGTCAGA GCAGTGACTT TGATACGCAG TCAGGTTTTT CCATTAATAG TCAGGTCTTT 
GCTGCAGATG GTGCCTCCAC TGAGACTTCC GCATCTGGGA CCTCCCAAGG AGAGGCTTCA 
ACTCCAGAGG AGTCTCGAGA TGGGAAGAAA GATAAAGAAG GGGACCGGGC CTCTGAGGAA 
GGCAAACAGA AAGGCAAGGG CAGCAAACCT TTAATGCCTA CCTCCACTAT CCTTCGTCTT 
CTGGCAGAGT TGGTGAGGTC CTATGTTGGT ATTGCTACCC TGATTGCCAA CTACAGCTAC 
ACTGTGGGCC AGTCTGAACT GATCAAAGAG GACTGCAGTG TGCTAGCTTT TGTTCTGGAC 
CACCTGCTCC CACATACCCA GAATGCAGAA GACAAGGACA CCCCTGCCTT GGCCCGCCTG 
TTCCTCGCAA GCCTGGCTGC TGCAGGGAGT GGCACAGATG CCCAGGTGGC CCTAGTGAAT 
GAAGTAAAAG CAGCCCTTGG ACGGGCACTG GCTATGGCTG AGAGTACAGA GAAACATGCC 
AGGCTTCAGG CAGTGATGTG TATCATCAGT ACTATCATGG AGTCCTGCCC CTCCACCTCC 
AGCTTCTACA GCAGTGCCAC AGCGAAGACC CAGCACAATG GCATGAACAA CATCATTCGG 
CTTTTCCTGA AGAAGGGACT GGTTAATGAC CTGGCCAGAG TACCTCACAG CTTAGACCTG 
TCCAGTCCCA ACATGGCCAA CACAGTCAAT GCTGCTCTGA AGCCTTTGGA AACACTTTCC 
CGGATTGTGA ACCAGCCCAG TAGCCTTTTT GGCAGCAAGA GTGCTTCTAG CAAGAACAAG 
TCTGAGCAGG ATGCCCAAGG AGCCTCTCAA GATTCCAGTA GCAACCAGCA GGACCCAGGC 
GAGCCTGGGG AAGCAGAAGT GCAGGAGGAG GATCATGATG TCACTCAGAC AGAGGTGGCA 
GATGGGGATA TCATGGATGG GGAGGCTGAA ACCGACTCAG TGGTGATTGC TGGGCAGCCT 
GAGGTGCTCA GTTCACAAGA GATGCAGGTT GAGAATGAGC TGGAGGACCT GATAGATGAG 
TTGCTTGAGA GGGATGGCGG ATCTGGGAAC AGTACAATTA TAGTGAGCAG AAGTGGAGAG 
GATGAATCAC AAGAGGACGT GCTGATGGAT GAAGCTCCTT CCAACCTCAG CCAAGCTTCC 
ACCTTGCAGG CCAACCGAGA AGATTCCATG AATATCCTGG ACCCTGAGGA TGAGGAGGAG 
CACACTCAGG AAGAGGACAG CAGTGGCAGT AACGAGGATG AGGATGATAG TCAGGATGAA 
GAGGAGGAGG AGGAGGAAGA TGAGGAAGAT GATCAGGAGG ATGATGAAGG TGAAGAGGGA 
GATGAAGACG ATGACGACGA TGGCTCTGAG ATGGAATTGG ATGAGGATTA TCCTGATATG 
AACGCTTCTC CCTTGGTCCG ATTTGAGCGC TTTGACCGGG AGGATGATCT CATCATTGAG 
TTTGACAACA TGTTCTCCAG TGCTACAGAC ATCCCCCCAT CCCCAGGAAA TATCCCTACC 
ACCCATCCAC TGATGGTGCG CCATGCAGAC CACAGTTCTC TGACACTGGG CAGTGGCTCT 
TCAACAACTC GTCTCACCCA GGGCATCGGG CGCAGTCAGA GGACCCTAAG GCAGCTGACG 
GCCAATACTG GCCACACCAT TCATGTTCAC TACCCTGGGA ATCGCCAGCC CAACCCTCCT 
CTTATACTGC AGAGGTTGCT TGGTCCCTCA GCTGCTGCTG ACATCCTTCA GCTGAGCAGC 
AGCCTTCCCC TACAAAGCCG GGGTCGGGCC CGCCTCCTGG TAGGCAACGA TGACGTCCAC 
ATCATCGCCC GTTCTGATGA TGAGCTGCTG GATGACTTTT TCCATGATCA GAGCACAGCT 
ACCAGCCAAG CAGGAACCCT GTCCAGCATC CCCACAGCCC TGACCCGCTG GACAGAAGAA 
TGCAAAGTTC TCGATGCTGA GAGCATGCAT GACTGTGTTT CAGTGGTTAA AGTGTCCATT 
GTCAATCACC TGGAATTCCT GAGGGATGAG GAGCTGGAAG AAAGGCGAGA GAAGCGCAGG 
AAACAACTGG CTGAGGAAGA AACAAAGATA ACTGATAAAG GCAAAGAAGA TAAGGAGAAC 
AGGGATCAGA GTGCACAGTG TACTGCATCT AAGTCAAATG ACTCCACTGA ACAGAATCTC 
TCAGATGGGA CGCCTATGCC TGACAGCTAC CCAACAACCC CATCTTCAAC TGATGCAGCT 
ACATCTGAGT CCAAGGAGAC CCTTGGCACT CTGCAATCCT CACAACAGCA ACCAACACTC 
CCAACCCCAC CAGCTTTGGG AGAGGTTCCT CAGGAGCTGC AGTCTCCAGC TGGAGAAGGG 
GGCAGCTCTA CACAGCTATT GATGCCTGTA GAGCCAGAGG AATTGGGTCC CACAAGGCCA 
AGTGGGGAAG CAGAAACAAC TCAGATGGAG TTATCCCCAG CTCCCACTAT AACCTCACTT 
TCCCCAGAGA GAGCTGAGGA TTCTGATGCA CTGACGGCTG TCAGCAGTCA GCTAGAAGGC 
TCTCCTATGG ATACAAGCAG CCTGGCTTCC TGTACCTTAG AGGAGGCTGT GGGTGACACT 
TCAGCAGCTG GCAGTTCTGA GCAGCCCAGA GCAGGCAGCT CCACTCCTGG GGATGCCCCA 
CCAGCTGTGG CGGAAGTGCA AGGCAGGAGT GATGGGTCAG GGGAATCTGC CCAGCCACCT 
GAGGACAGCT CCCCACCTGC ATCCTCTGAG AGCTCTTCCA CCAGAGATTC TGCCGTGGCC 
ATTTCTGGAG CAGATTCCCG AGGAATCCTA GAAGAGCCGT TGCCTTCAAC AAGCAGTGAA 
GAAGAAGATC CCCTTGCGGG TATCAGTCTC CCTGAAGGTG TGGACCCCTC TTTTCTGGCT 
GCCCTGCCTG ATGACATCCG TCGGGAAGTT CTACAGAACC AGCTAGGCAT TCGTCCACCA 
ACCCGGACTG CCCCCTCCAC AAATAGCTCA GCGCCTGCAG TGGTGGGGAA TCCTGGTGTG 
ACTGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029197.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884686.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X7, mRNA.

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CloneID	OHu99118
Clone ID Related Accession (Same CDS sequence)	XM_017029197.1
Accession Version	XM_017029197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GTTGCAGATG TGGCTATGGA AAGCAGTCCA GGCTCATCCA TCTCTATGGA GCACAGGCTG 
GATGTTGAAT TAAGGGCATC AGGTTCCAGC AGCAGCACTA ACATCTCTTC TGGCCCCAGC 
CCTGGTCCCA GTCCCGGCCC CGGCACCGGC CCTGGCCCCG GCCCCGGCCC CGGCCCCGGC 
CCTGGCCCCG GCCCCGGCCC CGGTCCTGGT CCCGGCCCTG GCCCCGGCCC TGGCCCTGGC 
CCCCGTCCTG GAGTCCAGTG TATTCCACAA CGAGCAGCAC TTCTGAAATC CATGTTGAAT 
TTCCTCAAGA AGGCCATCCA AGACCCTGCT TTCTCAGATG GCATACGACA TGTGATGGAT 
GGTTCTCTGC CTACCTCCCT GAAACACATC ATCAGCAATG CAGAATACTA TGGCCCATCA 
CTCTTCCTCC TAGCTACTGA AGTGGTGACT GTGTTTGTAT TTCAAGAACC ATCACTGCTC 
TCCTCACTCC AGGACAATGG ATTGACAGAT GTCATGCTGC ATGCACTGCT TATCAAAGAT 
GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC CTCCCAAATG TATTCAGTGC ACTCTGTTTG 
AATGCCCGAG GTCTTCAGTC TTTTGTTCAG TGTCAGCCTT TTGAACGCCT CTTCAAAGTT 
CTTCTGTCTC CAGATTACCT CCCAGCCATG CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG 
GATACTGCAT CCAACCTGGG GAGTGCTGTC GATGAGCTCA TGAGACATCA GCCCACCCTT 
AAAACAGATG CAACGACTGC CATCATCAAG TTACTTGAAG AAATCTGTAA TCTTGGAAGG 
GACCCCAAAT ACATCTGTCA GAAGCCATCA ATCCAGAAGG CAGATGGCAC TGCCACTGCT 
CCTCCCCCAA GGTCTAATCA TGCCGCAGAA GAAGCCTCTA GTGAGGATGA GGAGGAAGAG 
GAAGTACAGG CCATGCAGAG CTTTAATTCT ACCCAGCAAA ATGAAACTGA GCCTAATCAG 
CAGGTTGTTG GTACAGAGGA ACGTATTCCT ATTCCCCTCA TGGATTACAT CCTTAATGTG 
ATGAAATTTG TGGAATCTAT TCTGAGCAAC AATACAACAG ATGACCACTG CCAGGAATTT 
GTGAATCAGA AAGGACTGTT GCCTTTGGTT ACCATTTTGG GTCTTCCCAA TCTGCCCATT 
GACTTTCCCA CATCTGCTGC CTGTCAGGCT GTTGCAGGTG TCTGCAAATC CATATTGACA 
CTGTCACATG AACCCAAAGT CCTTCAAGAG GGTCTCCTTC AGTTGGACTC CATCCTCTCC 
TCCCTGGAGC CCTTACACCG CCCCATTGAA TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA 
CTGGCTTGCG CAGGCAATGT TGCTGATGCT ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG 
CATGCACTCA CTGCTGCCCA TGCCTACATC ATGATGTTTG TTCATACTTG CAGAGTTGGA 
CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC CAGTGGGGCT CTCAATTGGG TCTGAGTGTT 
TTGAGCAAGC TGAGCCAGTT ATACTGTTCC CTGGTGTGGG AAAGCACTGT CCTCCTCTCT 
CTGTGTACCC CAAACAGCCT ACCATCTGGG TGTGAATTTG GCCAGGCAGA TATGCAGAAA 
CTGGTTCCAA AGGATGAGAA GGCAGGTACG ACCCAGGGCG GAAAAAGATC AGATGGGGAA 
CAGGATGGAG CAGCTGGAAG TATGGATGCT TCTACCCAGG GCTTATTAGA AGGCATTGGG 
CTAGATGGTG ACACATTGGC TCCCATGGAG ACAGATGAAC CTACTGCTTC AGACTCTAAG 
GGCAAATCTA AAATCACACC AGCAATGGCT GCCAGAATTA AGCAAATCAA GCCTTTGTTA 
TCAGCTTCCT CCAGATTAGG CCGAGCACTT GCTGAGCTAT TTGGACTTCT TGTTAAACTT 
TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG AGCCATCATG CTGCCAGCAC CACTACAGCA 
CCGACACCTG CCGCGCGATC AACAGCCTCA GCTCTCACTA AGCTCTTGAC TAAGGGGTTA 
TCTTGGCAGC CCCCACCATA TACACCTACT CCCCGATTCA GGCTGACATT CTTCATCTGT 
TCAGTTGGTT TCACATCCCC AATGCTGTTT GATGAGAGGA AGTATCCCTA CCACCTCATG 
CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC AATGCTCTTT TTGAAACTTT CAACTGGGCT 
CTGTCCATGG GAGGTAAAGT TCCTGTTTCT GAGGGATTGG AACACTCAGA CTTGCCTGAT 
GGCACAGGAG AATTCCTAGA TGCCTGGCTT ATGCTGGTGG AGAAGATGGT GAATCCCACC 
ACGGTGCTTG AATCTCCACA TTCGCTGCCT GCCAAATTGC CTGGAGGTGT CCAGAACTTT 
CCCCAGTTCA GTGCACTGCG CTTCCTTGTG GTAACTCAGA AAGCAGCCTT TACTTGCATC 
AAAAACTTAT GGAACCGGAA ACCCCTGAAG GTATATGGTG GACGAATGGC TGAATCGATG 
CTGGCCATTC TATGCCACAT CCTCCGAGGA GAACCTGTGA TTCGAGAGAG ACTAAGCAAG 
GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG 
GAACCTCAAG TCAACCAGCA ACAACTGCAA CAGCTCATGG ACATGGGCTT CACAAGGGAA 
CATGCAATGG AGGCACTGTT GAACACCAGC ACCATGGAGC AGGCCACAGA GTACCTTTTA 
ACCCACCCTC CTCCAATCAT GGGAGGAGTT GTTCGGGATC TCAGCATGTC TGAAGAGGAC 
CAGATGATGA GAGCAATTGC TATGTCTCTG GGACAGGATA TTCCAATGGA TCAAAGGGCA 
GAGTCACCTG AGGAAGTTGC TTGCCGGAAG GAGGAAGAGG AACGGAAAGC TCGGGAAAAG 
CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG AAGTTCCAGG ATGCTGACCC GTTGGAACAA 
GATGAGCTCC ACACTTTCAC AGATACTATG TTGCCAGGCT GCTTCCACCT TCTTGATGAG 
CTGCCAGACA CAGTATACCG TGTGTGTGAC CTGATCATGA CAGCAATCAA ACGTAATGGA 
GCAGATTATC GTGACATGAT TCTGAAGCAA GTAGTCAATC AGGTGTGGGA AGCTGCTGAT 
GTATTGATCA AAGCTGCTCT TCCCCTGACA ACAAGTGACA CAAAAACCGT GTCAGAGTGG 
ATAAGTCAGA TGGCCACACT GCCCCAGGCC TCCAATTTGG CTACTAGAAT CTTGCTTTTA 
ACGCTACTTT TTGAGGAGTT GAAGCTACCT TGTGCTTGGG TGGTTGAATC AAGTGGCATC 
CTTAATGTCC TAATCAAACT CTTGGAAGTG GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG 
CAGAAGGAAG TCCAGACCCC AAAGTGGATC ACACCAGTGT TGCTCCTGAT TGATTTCTAT 
GAAAAGACAG CCATCTCCTC AAAAAGGAGA GCCCAGATGA CTAAGTACCT GCAATCCAAC 
AGCAACAACT GGCGCTGGTT TGATGATCGC TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC 
AACAATAGCA CTATTGATTC TGCCTGGAAA TCTGGAGAGA CAAGCGTGCG ATTCACTGCA 
GGCCGAAGAA GATACACGGT CCAATTCACT ACAATGGTGC AGGTTAATGA GGAAACAGGG 
AACCGACGCC CTGTGATGCT GACTCTCCTC AGGGTACCTC GGCTGAATAA AAATTCAAAA 
AACAGCAATG GACAGGAACT AGAGAAGACG CTGGAAGAAA GCAAAGAAAT GGATATCAAA 
CGTAAAGAAA ATAAAGGCAA TGATACCCCT TTGGCCCTAG AGAGTACAAA CACTGAAAAG 
GAGACAAGCC TGGAGGAAAC AAAAATCGGG GAGATCCTGA TCCAGGGCTT GACAGAAGAT 
ATGGTGACTG TTTTAATCCG GGCCTGCGTG AGCATGCTGG GAGTCCCTGT GGACCCAGAT 
ACTTTGCATG CCACCCTTCG TCTCTGTCTG AGGCTCACCC GGGACCACAA ATATGCCATG 
ATGTTTGCAG AACTGAAGAG TACCCGCATG ATCTTGAATT TGACCCAGAG CTCAGGCTTC 
AATGGGTTTA CTCCCCTGGT CACCCTTCTC TTAAGACACA TCATTGAGGA CCCCTGTACC 
CTTCGTCATA CCATGGAAAA GGTTGTTCGC TCAGCAGCTA CAAGTGGAGC TGGTAGCACT 
ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC TCTCGGGAGA TCAACTACAT CCTTCGTGTC 
CTTGGGCCAG CCGCATGCCG CAATCCAGAC ATATTCACAG AAGTGGCCAA CTGCTGTATC 
CGCATCGCCC TTCCTGCCCC TCGAGGCTCA GGAACTGCTT CAGATGATGA ATTTGAGAAT 
CTTAGAATTA AAGGCCCTAA TGCTGTACAG CTGGTGAAGA CCACCCCTTT GAAGCCCTCA 
CCTCTGCCTG TCATCCCTGA TACTATCAAG GAAGTGATCT ATGATATGCT GAATGCTCTG 
GCTGCATACC ATGCTCCAGA GGAAGCAGAT AAATCTGATC CTAAACCTGG GGTTATGACC 
CAAGAGGTTG GCCAGCTCCT GCAAGACATG GGTGATGATG TATACCAGCA GTACCGGTCA 
CTTACGCGTC AGAGCAGTGA CTTTGATACG CAGTCAGGTT TTTCCATTAA TAGTCAGGTC 
TTTGCTGCAG ATGGTGCCTC CACTGAGACT TCCGCATCTG GGACCTCCCA AGGAGAGGCT 
TCAACTCCAG AGGAGTCTCG AGATGGGAAG AAAGATAAAG AAGGGGACCG GGCCTCTGAG 
GAAGGCAAAC AGAAAGGCAA GGGCAGCAAA CCTTTAATGC CTACCTCCAC TATCCTTCGT 
CTTCTGGCAG AGTTGGTGAG GTCCTATGTT GGTATTGCTA CCCTGATTGC CAACTACAGC 
TACACTGTGG GCCAGTCTGA ACTGATCAAA GAGGACTGCA GTGTGCTAGC TTTTGTTCTG 
GACCACCTGC TCCCACATAC CCAGAATGCA GAAGACAAGG ACACCCCTGC CTTGGCCCGC 
CTGTTCCTCG CAAGCCTGGC TGCTGCAGGG AGTGGCACAG ATGCCCAGGT GGCCCTAGTG 
AATGAAGTAA AAGCAGCCCT TGGACGGGCA CTGGCTATGG CTGAGAGTAC AGAGAAACAT 
GCCAGGCTTC AGGCAGTGAT GTGTATCATC AGTACTATCA TGGAGTCCTG CCCCTCCACC 
TCCAGCTTCT ACAGCAGTGC CACAGCGAAG ACCCAGCACA ATGGCATGAA CAACATCATT 
CGGCTTTTCC TGAAGAAGGG ACTGGTTAAT GACCTGGCCA GAGTACCTCA CAGCTTAGAC 
CTGTCCAGTC CCAACATGGC CAACACAGTC AATGCTGCTC TGAAGCCTTT GGAAACACTT 
TCCCGGATTG TGAACCAGCC CAGTAGCCTT TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC 
AAGTCTGAGC AGGATGCCCA AGGAGCCTCT CAAGATTCCA GTAGCAACCA GCAGGACCCA 
GGCGAGCCTG GGGAAGCAGA AGTGCAGGAG GAGGATCATG ATGTCACTCA GACAGAGGTG 
GCAGATGGGG ATATCATGGA TGGGGAGGCT GAAACCGACT CAGTGGTGAT TGCTGGGCAG 
CCTGAGGTGC TCAGTTCACA AGAGATGCAG GTTGAGAATG AGCTGGAGGA CCTGATAGAT 
GAGTTGCTTG AGAGGGATGG CGGATCTGGG AACAGTACAA TTATAGATTC CATGAATATC 
CTGGACCCTG AGGATGAGGA GGAGCACACT CAGGAAGAGG ACAGCAGTGG CAGTAACGAG 
GATGAGGATG ATAGTCAGGA TGAAGAGGAG GAGGAGGAGG AAGATGAGGA AGATGATCAG 
GAGGATGATG AAGGTGAAGA GGGAGATGAA GACGATGACG ACGATGGCTC TGAGATGGAA 
TTGGATGAGG ATTATCCTGA TATGAACGCT TCTCCCTTGG TCCGATTTGA GCGCTTTGAC 
CGGGAGGATG ATCTCATCAT TGAGTTTGAC AACATGTTCT CCAGTGCTAC AGACATCCCC 
CCATCCCCAG GAAATATCCC TACCACCCAT CCACTGATGG TGCGCCATGC AGACCACAGT 
TCTCTGACAC TGGGCAGTGG CTCTTCAACA ACTCGTCTCA CCCAGGGCAT CGGGCGCAGT 
CAGAGGACCC TAAGGCAGCT GACGGCCAAT ACTGGCCACA CCATTCATGT TCACTACCCT 
GGGAATCGCC AGCCCAACCC TCCTCTTATA CTGCAGAGGT TGCTTGGTCC CTCAGCTGCT 
GCTGACATCC TTCAGCTGAG CAGCAGCCTT CCCCTACAAA GCCGGGGTCG GGCCCGCCTC 
CTGGTAGGCA ACGATGACGT CCACATCATC GCCCGTTCTG ATGATGAGCT GCTGGATGAC 
TTTTTCCATG ATCAGAGCAC AGCTACCAGC CAAGCAGGAA CCCTGTCCAG CATCCCCACA 
GCCCTGACCC GCTGGACAGA AGAATGCAAA GTTCTCGATG CTGAGAGCAT GCATGACTGT 
GTTTCAGTGG TTAAAGTGTC CATTGTCAAT CACCTGGAAT TCCTGAGGGA TGAGGAGCTG 
GAAGAAAGGC GAGAGAAGCG CAGGAAACAA CTGGCTGAGG AAGAAACAAA GATAACTGAT 
AAAGGCAAAG AAGATAAGGA GAACAGGGAT CAGAGTGCAC AGTGTACTGC ATCTAAGTCA 
AATGACTCCA CTGAACAGAA TCTCTCAGAT GGGACGCCTA TGCCTGACAG CTACCCAACA 
ACCCCATCTT CAACTGATGC AGCTACATCT GAGTCCAAGG AGACCCTTGG CACTCTGCAA 
TCCTCACAAC AGCAACCAAC ACTCCCAACC CCACCAGCTT TGGGAGAGGT TCCTCAGGAG 
CTGCAGTCTC CAGCTGGAGA AGGGGGCAGC TCTACACAGC TATTGATGCC TGTAGAGCCA 
GAGGAATTGG GTCCCACAAG GCCAAGTGGG GAAGCAGAAA CAACTCAGAT GGAGTTATCC 
CCAGCTCCCA CTATAACCTC ACTTTCCCCA GAGAGAGCTG AGGATTCTGA TGCACTGACG 
GCTGTCAGCA GTCAGCTAGA AGGCTCTCCT ATGGATACAA GCAGCCTGGC TTCCTGTACC 
TTAGAGGAGG CTGTGGGTGA CACTTCAGCA GCTGGCAGTT CTGAGCAGCC CAGAGCAGGC 
AGCTCCACTC CTGGGGATGC CCCACCAGCT GTGGCGGAAG TGCAAGGCAG GAGTGATGGG 
TCAGGGGAAT CTGCCCAGCC ACCTGAGGAC AGCTCCCCAC CTGCATCCTC TGAGAGCTCT 
TCCACCAGAG ATTCTGCCGT GGCCATTTCT GGAGCAGATT CCCGAGGAAT CCTAGAAGAG 
CCGTTGCCTT CAACAAGCAG TGAAGAAGAA GATCCCCTTG CGGGTATCAG TCTCCCTGAA 
GGTGTGGACC CCTCTTTTCT GGCTGCCCTG CCTGATGACA TCCGTCGGGA AGTTCTACAG 
AACCAGCTAG GCATTCGTCC ACCAACCCGG ACTGCCCCCT CCACAAATAG CTCAGCGCCT 
GCAGTGGTGG GGAATCCTGG TGTGACTGAA GTGAGCCCTG AGTTTCTGGC TGCCCTGCCT 
CCAGCCATTC AGGAGGAAGT ACTGGCACAG CAGAGAGCTG AGCAGCAGCG ACGAGAACTA 
GCACAGAATG CCAGCTCAGA CACCCCTATG GACCCTGTGA CCTTCATCCA GACTCTGCCC 
TCAGACCTGC GCCGTAGTGT CCTAGAGGAT ATGGAGGACA GTGTGTTAGC TGTGATGCCA 
CCTGACATTG CAGCTGAGGC TCAAGCCCTG AGACGAGAGC AAGAAGCCCG GCAGCGACAG 
CTCATGCATG AGCGTCTGTT TGGGCACAGT AGCACCTCCG CACTCTCTGC TATTCTCCGA 
AGCCCGGCTT TCACCAGTCG CTTAAGTGGC AACCGTGGGG TCCAGTATAC TCGCCTTGCT 
GTGCAGAGAG GTGGCACCTT CCAGATGGGG GGTAGCAGCA GCCATAACAG GCCTTCTGGC 
AGTAATGTAG ATACTCTCCT CCGCCTCCGA GGACGGCTCC TTCTGGACCA CGAAGCCCTT 
TCTTGTCTCT TGGTCCTACT TTTTGTGGAT GAGCCAAAGC TCAATACTAG CCGTCTACAC 
CGAGTACTGA GAAATCTCTG CTACCATGCC CAGACCCGCC ACTGGGTCAT CCGCAGTCTG 
CTCTCCATCT TGCAGCGCAG CAGTGAGAGT GAGCTATGCA TTGAAACACC CAAACTCACT 
ACAAGTGAGG AAAAGGGCAA AAAGTCGAGC AAGAGCTGTG GGTCAAGTAG CCATGAGAAC 
CGTCCCCTGG ACCTGCTACA CAAGATGGAG TCAAAGAGCT CCAACCAGCT TTCCTGGCTC 
TCAGTATCCA TGGATGCAGC CCTAGGCTGC AGGACTAATA TATTTCAGAT CCAGCGTTCA 
GGGGGGCGTA AACATACCGA GAAGCATGCA AGCGGTGGCT CCACCGTCCA CATCCATCCC 
CAAGCTGCTC CTGTTGTCTG CAGACACGTT TTGGATACAC TCATTCAATT GGCCAAGGTA 
TTTCCCAGCC ACTTCACACA GCAGCGGACC AAAGAAACAA ACTGTGAGAG TGATCGGGAA 
AGGGGCAATA AGGCCTGTAG CCCATGCTCC TCACAGTCCT CCAGCAGTGG CATTTGCACA 
GACTTCTGGG ACTTATTGGT AAAACTGGAC AACATGAATG TCAGCCGGAA AGGCAAGAAC 
TCCGTGAAGT CAGTGCCAGT GAGCGCTGGC GGTGAGGGGG AAACCTCTCC ATACAGCCTC 
GAGGCCTCTC CACTGGGGCA GCTCATGAAC ATGTTGTCAC ACCCAGTCAT CCGCCGGAGC 
TCTCTCTTAA CTGAGAAACT CCTCAGACTC CTTTCTCTCA TCTCAATTGC TCTCCCAGAA 
AACAAGGTGT CAGAAGCACA GGCTAATTCT GGCAGCGGTG CTTCCTCCAC CACCACTGCC 
ACCTCAACCA CATCTACCAC CACCACCACT GCCGCCTCCA CCACGCCCAC ACCCCCTACT 
GCACCCACCC CTGTCACTTC TGCTCCAGCC CTGGTTGCTG CCACGGCTAT TTCCACCATT 
GTCGTAGCTG CTTCGACCAC AGTGACTACC CCCACGACTG CTACCACTAC TGTTTCAATT 
TCTCCCACTA CTAAGGGCAG CAAATCTCCA GCGAAGGTGA GTGATGGGGG CAGCAGCAGT 
ACAGACTTTA AGATGGTGTC CTCTGGCCTC ACTGAAAACC AGCTACAGCT CTCTGTAGAG 
GTGTTGACAT CCCACTCTTG TTCTGAGGAA GGCTTAGAGG ATGCAGCCAA CGTACTACTG 
CAGCTCTCCC GGGGGGACTC TGGGACCCGG GACACTGTTC TCAAGCTGCT ACTGAATGGA 
GCCCGCCATC TGGGTTATAC CCTTTGTAAA CAAATAGGTA CCCTGCTGGC CGAGCTGCGG 
GAATACAACC TCGAGCAGCA GCGGCGAGCC CAATGTGAAA CCCTCTCTCC TGATGGCCTG 
CCTGAGGAGC AGCCACAGAC CACCAAGCTG AAGGGCAAAA TGCAGAGCAG GTTTGACATG 
GCTGAGAATG TGGTAATTGT GGCATCTCAG AAGCGACCTT TGGGTGGCCG GGAGCTCCAG 
CTGCCTTCTA TGTCCATGTT GACATCCAAG ACATCTACCC AGAAGTTCTT CTTGAGGGTA 
CTACAGGTCA TCATCCAGCT CCGGGACGAC ACGCGCCGGG CTAACAAGAA AGCCAAGCAG 
ACAGGCAGGC TAGGTTCCTC CGGTTTAGGC TCAGCTAGCA GCATCCAGGC AGCTGTTCGG 
CAGCTGGAGG CTGAGGCTGA TGCCATTATA CAAATGGTAC GTGAGGGTCA AAGGGCGCGG 
AGACAGCAAC AAGCAGCAAC GTCGGAGTCT AGCCAGTCAG AGGCGTCTGT CCGGAGGGAG 
GAATCACCCA TGGATGTGGA CCAGCCATCT CCCAGTGCTC AAGATACTCA ATCCATTGCC 
TCCGATGGAA CCCCACAGGG GGAGAAGGAA AAGGAAGAAA GACCACCTGA GTTACCCCTG 
CTCAGCGAGC AGCTGAGTTT GGACGAGCTG TGGGACATGC TTGGGGAGTG TCTAAAGGAA 
CTAGAGGAAT CCCATGACCA GCATGCGGTG CTAGTGCTAC AGCCTGCTGT CGAGGCCTTC 
TTTCTGGTCC ATGCCACAGA GCGGGAGAGC AAGCCTCCTG TCCGAGACAC CCGTGAGAGC 
CAGCTGGCAC ACATCAAGGA CGAGCCTCCT CCACTCTCCC CTGCCCCCTT AACCCCAGCC 
ACGCCTTCCT CCCTTGACCC ATTCTTCTCC CGGGAGCCCT CATCTATGCA CATCTCCTCA 
AGCCTGCCCC CTGACACACA GAAGTTCCTT CGCTTTGCAG AGACTCACCG CACTGTGTTA 
AACCAGATCC TACGGCAGTC CACGACCCAC CTTGCTGATG GGCCTTTTGC TGTCCTGGTA 
GACTACATTC GTGTCCTCGA CTTTGATGTC AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG 
CGTTTAGATG AGGGGCTCCG GAAAGAAGAC ATGGCTGTGC ATGTCCGTCG TGACCATGTG 
TTTGAAGACT CCTATCGTGA GCTGCATCGC AAATCCCCCG AAGAAATGAA GAATCGATTG 
TATATAGTAT TTGAAGGAGA AGAAGGGCAG GATGCTGGTG GGCTCCTGCG GGAGTGGTAT 
ATGATCATCT CTCGAGAGAT GTTTAACCCT ATGTATGCCT TGTTCCGTAC CTCACCTGGT 
GATCGAGTCA CCTACACCAT CAATCCATCT TCCCACTGCA ACCCCAACCA CCTCAGCTAC 
TTCAAGTTTG TCGGACGCAT TGTGGCCAAA GCTGTATATG ACAACCGTCT TCTGGAGTGC 
TACTTTACTC GATCCTTTTA CAAACACATC TTGGGCAAGT CAGTCAGATA TACAGATATG 
GAGAGTGAAG ATTACCACTT CTACCAAGGT CTGGTTTATC TGCTGGAAAA TGATGTCTCC 
ACACTAGGCT ATGACCTCAC CTTCAGCACT GAGGTCCAAG AGTTTGGAGT TTGTGAAGTT 
CGTGACCTCA AACCCAATGG GGCCAACATC TTGGTAACAG AGGAGAATAA GAAGGAGTAT 
GTACACCTGG TATGCCAGAT GAGAATGACA GGAGCCATCC GCAAGCAGTT GGCGGCTTTC 
TTAGAAGGCT TCTATGAGAT CATTCCAAAG CGCCTCATTT CCATCTTCAC TGAGCAGGAG 
TTAGAGCTGC TTATATCAGG ACTGCCCACC ATTGACATCG ATGATCTGAA ATCCAACACT 
GAATACCACA AGTACCAGTC CAACTCTATT CAGATCCAGT GGTTCTGGAG AGCATTGCGT 
TCTTTCGATC AAGCTGACCG TGCCAAGTTC CTCCAGTTTG TCACAGGTAC TTCCAAGGTA 
CCCCTGCAAG GCTTTGCTGC CCTCGAAGGC ATGAATGGCA TTCAGAAGTT TCAGATCCAT 
CGAGATGACA GGTCCACAGA TCGCCTGCCT TCAGCTCACA CATGTTTTAA TCAGCTGGAT 
CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC CGCCACATGC TACTGTTGGC TATCCAGGAG 
TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884686.1
CDS	3018..16424
Translation	MDPFISRREQKCMEIGTPRKNGVLVLRSSEAVYHLTTPFQIVCLHLPLSKSKPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029197.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029197.1

ORF Insert Sequence:

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GTTGCAGATG TGGCTATGGA AAGCAGTCCA GGCTCATCCA TCTCTATGGA GCACAGGCTG 
GATGTTGAAT TAAGGGCATC AGGTTCCAGC AGCAGCACTA ACATCTCTTC TGGCCCCAGC 
CCTGGTCCCA GTCCCGGCCC CGGCACCGGC CCTGGCCCCG GCCCCGGCCC CGGCCCCGGC 
CCTGGCCCCG GCCCCGGCCC CGGTCCTGGT CCCGGCCCTG GCCCCGGCCC TGGCCCTGGC 
CCCCGTCCTG GAGTCCAGTG TATTCCACAA CGAGCAGCAC TTCTGAAATC CATGTTGAAT 
TTCCTCAAGA AGGCCATCCA AGACCCTGCT TTCTCAGATG GCATACGACA TGTGATGGAT 
GGTTCTCTGC CTACCTCCCT GAAACACATC ATCAGCAATG CAGAATACTA TGGCCCATCA 
CTCTTCCTCC TAGCTACTGA AGTGGTGACT GTGTTTGTAT TTCAAGAACC ATCACTGCTC 
TCCTCACTCC AGGACAATGG ATTGACAGAT GTCATGCTGC ATGCACTGCT TATCAAAGAT 
GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC CTCCCAAATG TATTCAGTGC ACTCTGTTTG 
AATGCCCGAG GTCTTCAGTC TTTTGTTCAG TGTCAGCCTT TTGAACGCCT CTTCAAAGTT 
CTTCTGTCTC CAGATTACCT CCCAGCCATG CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG 
GATACTGCAT CCAACCTGGG GAGTGCTGTC GATGAGCTCA TGAGACATCA GCCCACCCTT 
AAAACAGATG CAACGACTGC CATCATCAAG TTACTTGAAG AAATCTGTAA TCTTGGAAGG 
GACCCCAAAT ACATCTGTCA GAAGCCATCA ATCCAGAAGG CAGATGGCAC TGCCACTGCT 
CCTCCCCCAA GGTCTAATCA TGCCGCAGAA GAAGCCTCTA GTGAGGATGA GGAGGAAGAG 
GAAGTACAGG CCATGCAGAG CTTTAATTCT ACCCAGCAAA ATGAAACTGA GCCTAATCAG 
CAGGTTGTTG GTACAGAGGA ACGTATTCCT ATTCCCCTCA TGGATTACAT CCTTAATGTG 
ATGAAATTTG TGGAATCTAT TCTGAGCAAC AATACAACAG ATGACCACTG CCAGGAATTT 
GTGAATCAGA AAGGACTGTT GCCTTTGGTT ACCATTTTGG GTCTTCCCAA TCTGCCCATT 
GACTTTCCCA CATCTGCTGC CTGTCAGGCT GTTGCAGGTG TCTGCAAATC CATATTGACA 
CTGTCACATG AACCCAAAGT CCTTCAAGAG GGTCTCCTTC AGTTGGACTC CATCCTCTCC 
TCCCTGGAGC CCTTACACCG CCCCATTGAA TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA 
CTGGCTTGCG CAGGCAATGT TGCTGATGCT ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG 
CATGCACTCA CTGCTGCCCA TGCCTACATC ATGATGTTTG TTCATACTTG CAGAGTTGGA 
CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC CAGTGGGGCT CTCAATTGGG TCTGAGTGTT 
TTGAGCAAGC TGAGCCAGTT ATACTGTTCC CTGGTGTGGG AAAGCACTGT CCTCCTCTCT 
CTGTGTACCC CAAACAGCCT ACCATCTGGG TGTGAATTTG GCCAGGCAGA TATGCAGAAA 
CTGGTTCCAA AGGATGAGAA GGCAGGTACG ACCCAGGGCG GAAAAAGATC AGATGGGGAA 
CAGGATGGAG CAGCTGGAAG TATGGATGCT TCTACCCAGG GCTTATTAGA AGGCATTGGG 
CTAGATGGTG ACACATTGGC TCCCATGGAG ACAGATGAAC CTACTGCTTC AGACTCTAAG 
GGCAAATCTA AAATCACACC AGCAATGGCT GCCAGAATTA AGCAAATCAA GCCTTTGTTA 
TCAGCTTCCT CCAGATTAGG CCGAGCACTT GCTGAGCTAT TTGGACTTCT TGTTAAACTT 
TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG AGCCATCATG CTGCCAGCAC CACTACAGCA 
CCGACACCTG CCGCGCGATC AACAGCCTCA GCTCTCACTA AGCTCTTGAC TAAGGGGTTA 
TCTTGGCAGC CCCCACCATA TACACCTACT CCCCGATTCA GGCTGACATT CTTCATCTGT 
TCAGTTGGTT TCACATCCCC AATGCTGTTT GATGAGAGGA AGTATCCCTA CCACCTCATG 
CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC AATGCTCTTT TTGAAACTTT CAACTGGGCT 
CTGTCCATGG GAGGTAAAGT TCCTGTTTCT GAGGGATTGG AACACTCAGA CTTGCCTGAT 
GGCACAGGAG AATTCCTAGA TGCCTGGCTT ATGCTGGTGG AGAAGATGGT GAATCCCACC 
ACGGTGCTTG AATCTCCACA TTCGCTGCCT GCCAAATTGC CTGGAGGTGT CCAGAACTTT 
CCCCAGTTCA GTGCACTGCG CTTCCTTGTG GTAACTCAGA AAGCAGCCTT TACTTGCATC 
AAAAACTTAT GGAACCGGAA ACCCCTGAAG GTATATGGTG GACGAATGGC TGAATCGATG 
CTGGCCATTC TATGCCACAT CCTCCGAGGA GAACCTGTGA TTCGAGAGAG ACTAAGCAAG 
GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG 
GAACCTCAAG TCAACCAGCA ACAACTGCAA CAGCTCATGG ACATGGGCTT CACAAGGGAA 
CATGCAATGG AGGCACTGTT GAACACCAGC ACCATGGAGC AGGCCACAGA GTACCTTTTA 
ACCCACCCTC CTCCAATCAT GGGAGGAGTT GTTCGGGATC TCAGCATGTC TGAAGAGGAC 
CAGATGATGA GAGCAATTGC TATGTCTCTG GGACAGGATA TTCCAATGGA TCAAAGGGCA 
GAGTCACCTG AGGAAGTTGC TTGCCGGAAG GAGGAAGAGG AACGGAAAGC TCGGGAAAAG 
CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG AAGTTCCAGG ATGCTGACCC GTTGGAACAA 
GATGAGCTCC ACACTTTCAC AGATACTATG TTGCCAGGCT GCTTCCACCT TCTTGATGAG 
CTGCCAGACA CAGTATACCG TGTGTGTGAC CTGATCATGA CAGCAATCAA ACGTAATGGA 
GCAGATTATC GTGACATGAT TCTGAAGCAA GTAGTCAATC AGGTGTGGGA AGCTGCTGAT 
GTATTGATCA AAGCTGCTCT TCCCCTGACA ACAAGTGACA CAAAAACCGT GTCAGAGTGG 
ATAAGTCAGA TGGCCACACT GCCCCAGGCC TCCAATTTGG CTACTAGAAT CTTGCTTTTA 
ACGCTACTTT TTGAGGAGTT GAAGCTACCT TGTGCTTGGG TGGTTGAATC AAGTGGCATC 
CTTAATGTCC TAATCAAACT CTTGGAAGTG GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG 
CAGAAGGAAG TCCAGACCCC AAAGTGGATC ACACCAGTGT TGCTCCTGAT TGATTTCTAT 
GAAAAGACAG CCATCTCCTC AAAAAGGAGA GCCCAGATGA CTAAGTACCT GCAATCCAAC 
AGCAACAACT GGCGCTGGTT TGATGATCGC TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC 
AACAATAGCA CTATTGATTC TGCCTGGAAA TCTGGAGAGA CAAGCGTGCG ATTCACTGCA 
GGCCGAAGAA GATACACGGT CCAATTCACT ACAATGGTGC AGGTTAATGA GGAAACAGGG 
AACCGACGCC CTGTGATGCT GACTCTCCTC AGGGTACCTC GGCTGAATAA AAATTCAAAA 
AACAGCAATG GACAGGAACT AGAGAAGACG CTGGAAGAAA GCAAAGAAAT GGATATCAAA 
CGTAAAGAAA ATAAAGGCAA TGATACCCCT TTGGCCCTAG AGAGTACAAA CACTGAAAAG 
GAGACAAGCC TGGAGGAAAC AAAAATCGGG GAGATCCTGA TCCAGGGCTT GACAGAAGAT 
ATGGTGACTG TTTTAATCCG GGCCTGCGTG AGCATGCTGG GAGTCCCTGT GGACCCAGAT 
ACTTTGCATG CCACCCTTCG TCTCTGTCTG AGGCTCACCC GGGACCACAA ATATGCCATG 
ATGTTTGCAG AACTGAAGAG TACCCGCATG ATCTTGAATT TGACCCAGAG CTCAGGCTTC 
AATGGGTTTA CTCCCCTGGT CACCCTTCTC TTAAGACACA TCATTGAGGA CCCCTGTACC 
CTTCGTCATA CCATGGAAAA GGTTGTTCGC TCAGCAGCTA CAAGTGGAGC TGGTAGCACT 
ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC TCTCGGGAGA TCAACTACAT CCTTCGTGTC 
CTTGGGCCAG CCGCATGCCG CAATCCAGAC ATATTCACAG AAGTGGCCAA CTGCTGTATC 
CGCATCGCCC TTCCTGCCCC TCGAGGCTCA GGAACTGCTT CAGATGATGA ATTTGAGAAT 
CTTAGAATTA AAGGCCCTAA TGCTGTACAG CTGGTGAAGA CCACCCCTTT GAAGCCCTCA 
CCTCTGCCTG TCATCCCTGA TACTATCAAG GAAGTGATCT ATGATATGCT GAATGCTCTG 
GCTGCATACC ATGCTCCAGA GGAAGCAGAT AAATCTGATC CTAAACCTGG GGTTATGACC 
CAAGAGGTTG GCCAGCTCCT GCAAGACATG GGTGATGATG TATACCAGCA GTACCGGTCA 
CTTACGCGTC AGAGCAGTGA CTTTGATACG CAGTCAGGTT TTTCCATTAA TAGTCAGGTC 
TTTGCTGCAG ATGGTGCCTC CACTGAGACT TCCGCATCTG GGACCTCCCA AGGAGAGGCT 
TCAACTCCAG AGGAGTCTCG AGATGGGAAG AAAGATAAAG AAGGGGACCG GGCCTCTGAG 
GAAGGCAAAC AGAAAGGCAA GGGCAGCAAA CCTTTAATGC CTACCTCCAC TATCCTTCGT 
CTTCTGGCAG AGTTGGTGAG GTCCTATGTT GGTATTGCTA CCCTGATTGC CAACTACAGC 
TACACTGTGG GCCAGTCTGA ACTGATCAAA GAGGACTGCA GTGTGCTAGC TTTTGTTCTG 
GACCACCTGC TCCCACATAC CCAGAATGCA GAAGACAAGG ACACCCCTGC CTTGGCCCGC 
CTGTTCCTCG CAAGCCTGGC TGCTGCAGGG AGTGGCACAG ATGCCCAGGT GGCCCTAGTG 
AATGAAGTAA AAGCAGCCCT TGGACGGGCA CTGGCTATGG CTGAGAGTAC AGAGAAACAT 
GCCAGGCTTC AGGCAGTGAT GTGTATCATC AGTACTATCA TGGAGTCCTG CCCCTCCACC 
TCCAGCTTCT ACAGCAGTGC CACAGCGAAG ACCCAGCACA ATGGCATGAA CAACATCATT 
CGGCTTTTCC TGAAGAAGGG ACTGGTTAAT GACCTGGCCA GAGTACCTCA CAGCTTAGAC 
CTGTCCAGTC CCAACATGGC CAACACAGTC AATGCTGCTC TGAAGCCTTT GGAAACACTT 
TCCCGGATTG TGAACCAGCC CAGTAGCCTT TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC 
AAGTCTGAGC AGGATGCCCA AGGAGCCTCT CAAGATTCCA GTAGCAACCA GCAGGACCCA 
GGCGAGCCTG GGGAAGCAGA AGTGCAGGAG GAGGATCATG ATGTCACTCA GACAGAGGTG 
GCAGATGGGG ATATCATGGA TGGGGAGGCT GAAACCGACT CAGTGGTGAT TGCTGGGCAG 
CCTGAGGTGC TCAGTTCACA AGAGATGCAG GTTGAGAATG AGCTGGAGGA CCTGATAGAT 
GAGTTGCTTG AGAGGGATGG CGGATCTGGG AACAGTACAA TTATAGATTC CATGAATATC 
CTGGACCCTG AGGATGAGGA GGAGCACACT CAGGAAGAGG ACAGCAGTGG CAGTAACGAG 
GATGAGGATG ATAGTCAGGA TGAAGAGGAG GAGGAGGAGG AAGATGAGGA AGATGATCAG 
GAGGATGATG AAGGTGAAGA GGGAGATGAA GACGATGACG ACGATGGCTC TGAGATGGAA 
TTGGATGAGG ATTATCCTGA TATGAACGCT TCTCCCTTGG TCCGATTTGA GCGCTTTGAC 
CGGGAGGATG ATCTCATCAT TGAGTTTGAC AACATGTTCT CCAGTGCTAC AGACATCCCC 
CCATCCCCAG GAAATATCCC TACCACCCAT CCACTGATGG TGCGCCATGC AGACCACAGT 
TCTCTGACAC TGGGCAGTGG CTCTTCAACA ACTCGTCTCA CCCAGGGCAT CGGGCGCAGT 
CAGAGGACCC TAAGGCAGCT GACGGCCAAT ACTGGCCACA CCATTCATGT TCACTACCCT 
GGGAATCGCC AGCCCAACCC TCCTCTTATA CTGCAGAGGT TGCTTGGTCC CTCAGCTGCT 
GCTGACATCC TTCAGCTGAG CAGCAGCCTT CCCCTACAAA GCCGGGGTCG GGCCCGCCTC 
CTGGTAGGCA ACGATGACGT CCACATCATC GCCCGTTCTG ATGATGAGCT GCTGGATGAC 
TTTTTCCATG ATCAGAGCAC AGCTACCAGC CAAGCAGGAA CCCTGTCCAG CATCCCCACA 
GCCCTGACCC GCTGGACAGA AGAATGCAAA GTTCTCGATG CTGAGAGCAT GCATGACTGT 
GTTTCAGTGG TTAAAGTGTC CATTGTCAAT CACCTGGAAT TCCTGAGGGA TGAGGAGCTG 
GAAGAAAGGC GAGAGAAGCG CAGGAAACAA CTGGCTGAGG AAGAAACAAA GATAACTGAT 
AAAGGCAAAG AAGATAAGGA GAACAGGGAT CAGAGTGCAC AGTGTACTGC ATCTAAGTCA 
AATGACTCCA CTGAACAGAA TCTCTCAGAT GGGACGCCTA TGCCTGACAG CTACCCAACA 
ACCCCATCTT CAACTGATGC AGCTACATCT GAGTCCAAGG AGACCCTTGG CACTCTGCAA 
TCCTCACAAC AGCAACCAAC ACTCCCAACC CCACCAGCTT TGGGAGAGGT TCCTCAGGAG 
CTGCAGTCTC CAGCTGGAGA AGGGGGCAGC TCTACACAGC TATTGATGCC TGTAGAGCCA 
GAGGAATTGG GTCCCACAAG GCCAAGTGGG GAAGCAGAAA CAACTCAGAT GGAGTTATCC 
CCAGCTCCCA CTATAACCTC ACTTTCCCCA GAGAGAGCTG AGGATTCTGA TGCACTGACG 
GCTGTCAGCA GTCAGCTAGA AGGCTCTCCT ATGGATACAA GCAGCCTGGC TTCCTGTACC 
TTAGAGGAGG CTGTGGGTGA CACTTCAGCA GCTGGCAGTT CTGAGCAGCC CAGAGCAGGC 
AGCTCCACTC CTGGGGATGC CCCACCAGCT GTGGCGGAAG TGCAAGGCAG GAGTGATGGG 
TCAGGGGAAT CTGCCCAGCC ACCTGAGGAC AGCTCCCCAC CTGCATCCTC TGAGAGCTCT 
TCCACCAGAG ATTCTGCCGT GGCCATTTCT GGAGCAGATT CCCGAGGAAT CCTAGAAGAG 
CCGTTGCCTT CAACAAGCAG TGAAGAAGAA GATCCCCTTG CGGGTATCAG TCTCCCTGAA 
GGTGTGGACC CCTCTTTTCT GGCTGCCCTG CCTGATGACA TCCGTCGGGA AGTTCTACAG 
AACCAGCTAG GCATTCGTCC ACCAACCCGG ACTGCCCCCT CCACAAATAG CTCAGCGCCT 
GCAGTGGTGG GGAATCCTGG TGTGACTGAA GTGAGCCCTG AGTTTCTGGC TGCCCTGCCT 
CCAGCCATTC AGGAGGAAGT ACTGGCACAG CAGAGAGCTG AGCAGCAGCG ACGAGAACTA 
GCACAGAATG CCAGCTCAGA CACCCCTATG GACCCTGTGA CCTTCATCCA GACTCTGCCC 
TCAGACCTGC GCCGTAGTGT CCTAGAGGAT ATGGAGGACA GTGTGTTAGC TGTGATGCCA 
CCTGACATTG CAGCTGAGGC TCAAGCCCTG AGACGAGAGC AAGAAGCCCG GCAGCGACAG 
CTCATGCATG AGCGTCTGTT TGGGCACAGT AGCACCTCCG CACTCTCTGC TATTCTCCGA 
AGCCCGGCTT TCACCAGTCG CTTAAGTGGC AACCGTGGGG TCCAGTATAC TCGCCTTGCT 
GTGCAGAGAG GTGGCACCTT CCAGATGGGG GGTAGCAGCA GCCATAACAG GCCTTCTGGC 
AGTAATGTAG ATACTCTCCT CCGCCTCCGA GGACGGCTCC TTCTGGACCA CGAAGCCCTT 
TCTTGTCTCT TGGTCCTACT TTTTGTGGAT GAGCCAAAGC TCAATACTAG CCGTCTACAC 
CGAGTACTGA GAAATCTCTG CTACCATGCC CAGACCCGCC ACTGGGTCAT CCGCAGTCTG 
CTCTCCATCT TGCAGCGCAG CAGTGAGAGT GAGCTATGCA TTGAAACACC CAAACTCACT 
ACAAGTGAGG AAAAGGGCAA AAAGTCGAGC AAGAGCTGTG GGTCAAGTAG CCATGAGAAC 
CGTCCCCTGG ACCTGCTACA CAAGATGGAG TCAAAGAGCT CCAACCAGCT TTCCTGGCTC 
TCAGTATCCA TGGATGCAGC CCTAGGCTGC AGGACTAATA TATTTCAGAT CCAGCGTTCA 
GGGGGGCGTA AACATACCGA GAAGCATGCA AGCGGTGGCT CCACCGTCCA CATCCATCCC 
CAAGCTGCTC CTGTTGTCTG CAGACACGTT TTGGATACAC TCATTCAATT GGCCAAGGTA 
TTTCCCAGCC ACTTCACACA GCAGCGGACC AAAGAAACAA ACTGTGAGAG TGATCGGGAA 
AGGGGCAATA AGGCCTGTAG CCCATGCTCC TCACAGTCCT CCAGCAGTGG CATTTGCACA 
GACTTCTGGG ACTTATTGGT AAAACTGGAC AACATGAATG TCAGCCGGAA AGGCAAGAAC 
TCCGTGAAGT CAGTGCCAGT GAGCGCTGGC GGTGAGGGGG AAACCTCTCC ATACAGCCTC 
GAGGCCTCTC CACTGGGGCA GCTCATGAAC ATGTTGTCAC ACCCAGTCAT CCGCCGGAGC 
TCTCTCTTAA CTGAGAAACT CCTCAGACTC CTTTCTCTCA TCTCAATTGC TCTCCCAGAA 
AACAAGGTGT CAGAAGCACA GGCTAATTCT GGCAGCGGTG CTTCCTCCAC CACCACTGCC 
ACCTCAACCA CATCTACCAC CACCACCACT GCCGCCTCCA CCACGCCCAC ACCCCCTACT 
GCACCCACCC CTGTCACTTC TGCTCCAGCC CTGGTTGCTG CCACGGCTAT TTCCACCATT 
GTCGTAGCTG CTTCGACCAC AGTGACTACC CCCACGACTG CTACCACTAC TGTTTCAATT 
TCTCCCACTA CTAAGGGCAG CAAATCTCCA GCGAAGGTGA GTGATGGGGG CAGCAGCAGT 
ACAGACTTTA AGATGGTGTC CTCTGGCCTC ACTGAAAACC AGCTACAGCT CTCTGTAGAG 
GTGTTGACAT CCCACTCTTG TTCTGAGGAA GGCTTAGAGG ATGCAGCCAA CGTACTACTG 
CAGCTCTCCC GGGGGGACTC TGGGACCCGG GACACTGTTC TCAAGCTGCT ACTGAATGGA 
GCCCGCCATC TGGGTTATAC CCTTTGTAAA CAAATAGGTA CCCTGCTGGC CGAGCTGCGG 
GAATACAACC TCGAGCAGCA GCGGCGAGCC CAATGTGAAA CCCTCTCTCC TGATGGCCTG 
CCTGAGGAGC AGCCACAGAC CACCAAGCTG AAGGGCAAAA TGCAGAGCAG GTTTGACATG 
GCTGAGAATG TGGTAATTGT GGCATCTCAG AAGCGACCTT TGGGTGGCCG GGAGCTCCAG 
CTGCCTTCTA TGTCCATGTT GACATCCAAG ACATCTACCC AGAAGTTCTT CTTGAGGGTA 
CTACAGGTCA TCATCCAGCT CCGGGACGAC ACGCGCCGGG CTAACAAGAA AGCCAAGCAG 
ACAGGCAGGC TAGGTTCCTC CGGTTTAGGC TCAGCTAGCA GCATCCAGGC AGCTGTTCGG 
CAGCTGGAGG CTGAGGCTGA TGCCATTATA CAAATGGTAC GTGAGGGTCA AAGGGCGCGG 
AGACAGCAAC AAGCAGCAAC GTCGGAGTCT AGCCAGTCAG AGGCGTCTGT CCGGAGGGAG 
GAATCACCCA TGGATGTGGA CCAGCCATCT CCCAGTGCTC AAGATACTCA ATCCATTGCC 
TCCGATGGAA CCCCACAGGG GGAGAAGGAA AAGGAAGAAA GACCACCTGA GTTACCCCTG 
CTCAGCGAGC AGCTGAGTTT GGACGAGCTG TGGGACATGC TTGGGGAGTG TCTAAAGGAA 
CTAGAGGAAT CCCATGACCA GCATGCGGTG CTAGTGCTAC AGCCTGCTGT CGAGGCCTTC 
TTTCTGGTCC ATGCCACAGA GCGGGAGAGC AAGCCTCCTG TCCGAGACAC CCGTGAGAGC 
CAGCTGGCAC ACATCAAGGA CGAGCCTCCT CCACTCTCCC CTGCCCCCTT AACCCCAGCC 
ACGCCTTCCT CCCTTGACCC ATTCTTCTCC CGGGAGCCCT CATCTATGCA CATCTCCTCA 
AGCCTGCCCC CTGACACACA GAAGTTCCTT CGCTTTGCAG AGACTCACCG CACTGTGTTA 
AACCAGATCC TACGGCAGTC CACGACCCAC CTTGCTGATG GGCCTTTTGC TGTCCTGGTA 
GACTACATTC GTGTCCTCGA CTTTGATGTC AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG 
CGTTTAGATG AGGGGCTCCG GAAAGAAGAC ATGGCTGTGC ATGTCCGTCG TGACCATGTG 
TTTGAAGACT CCTATCGTGA GCTGCATCGC AAATCCCCCG AAGAAATGAA GAATCGATTG 
TATATAGTAT TTGAAGGAGA AGAAGGGCAG GATGCTGGTG GGCTCCTGCG GGAGTGGTAT 
ATGATCATCT CTCGAGAGAT GTTTAACCCT ATGTATGCCT TGTTCCGTAC CTCACCTGGT 
GATCGAGTCA CCTACACCAT CAATCCATCT TCCCACTGCA ACCCCAACCA CCTCAGCTAC 
TTCAAGTTTG TCGGACGCAT TGTGGCCAAA GCTGTATATG ACAACCGTCT TCTGGAGTGC 
TACTTTACTC GATCCTTTTA CAAACACATC TTGGGCAAGT CAGTCAGATA TACAGATATG 
GAGAGTGAAG ATTACCACTT CTACCAAGGT CTGGTTTATC TGCTGGAAAA TGATGTCTCC 
ACACTAGGCT ATGACCTCAC CTTCAGCACT GAGGTCCAAG AGTTTGGAGT TTGTGAAGTT 
CGTGACCTCA AACCCAATGG GGCCAACATC TTGGTAACAG AGGAGAATAA GAAGGAGTAT 
GTACACCTGG TATGCCAGAT GAGAATGACA GGAGCCATCC GCAAGCAGTT GGCGGCTTTC 
TTAGAAGGCT TCTATGAGAT CATTCCAAAG CGCCTCATTT CCATCTTCAC TGAGCAGGAG 
TTAGAGCTGC TTATATCAGG ACTGCCCACC ATTGACATCG ATGATCTGAA ATCCAACACT 
GAATACCACA AGTACCAGTC CAACTCTATT CAGATCCAGT GGTTCTGGAG AGCATTGCGT 
TCTTTCGATC AAGCTGACCG TGCCAAGTTC CTCCAGTTTG TCACAGGTAC TTCCAAGGTA 
CCCCTGCAAG GCTTTGCTGC CCTCGAAGGC ATGAATGGCA TTCAGAAGTT TCAGATCCAT 
CGAGATGACA GGTCCACAGA TCGCCTGCCT TCAGCTCACA CATGTTTTAA TCAGCTGGAT 
CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC CGCCACATGC TACTGTTGGC TATCCAGGAG 
TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029199.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884688.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X9, mRNA.

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CloneID	OHu99119
Clone ID Related Accession (Same CDS sequence)	XM_017029199.1 , XM_017029201.1 , XM_017029203.1 , XM_017029200.1 , XM_017029202.1 , XM_017029198.1 , XM_017029198.2
Accession Version	XM_017029199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTTTGTT TCTCCTTTTG CAGTGTATTC CAATGCCTTG 
CAGGAATCAG CAAACAGTAT CTTGTATAAT GGCTTGATAG AGGAGTTGGT AGATGTCCTT 
CAGATAACGG ATAAGCAGCT TATGGAGATT AAAGCAGCTT CTTTACGAAC ATTAACATCA 
ATTGTCCACT TGGAGAGAAC TCCCAAACTC AGCAGTATTA TTGACTGTAC TGGAACTGCC 
TCCTACCATG GATTTTTGCC AGTGCTTGTA AGGAACTGTA TCCAGGCCAT GATTGATCCT 
TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG 
GCCAGCTACG ATGCTGGTGG TGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG 
AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCCGTC 
AGAGTGGTTG ACCTTATCAC CAACCTGGAT ATGGCAGCTT TTCAATCCCA TAGTGGACTT 
TCTATCTTCA TTTATAGACT TGAGCATGAA GTAGATTTGT GCCGAAAAGA ATGTCCGTTT 
GTGATCAAGC CAAAGATCCA GAGACCCAAT ACTACACAAG AAGGAGAGGA AATGGAAACT 
GATATGGATG TTGCAGATGT GGCTATGGAA AGCAGTCCAG GCTCATCCAT CTCTATGGAG 
CACAGGCTGG ATGTTGAATT AAGGGCATCA GGTTCCAGCA GCAGCACTAA CATCTCTTCT 
GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC GGCACCGGCC CTGGCCCCGG CCCCGGCCCC 
GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT 
GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT ATTCCACAAC GAGCAGCACT TCTGAAATCC 
ATGTTGAATT TCCTCAAGAA GGCCATCCAA GACCCTGCTT TCTCAGATGG CATACGACAT 
GTGATGGATG GTTCTCTGCC TACCTCCCTG AAACACATCA TCAGCAATGC AGAATACTAT 
GGCCCATCAC TCTTCCTCCT AGCTACTGAA GTGGTGACTG TGTTTGTATT TCAAGAACCA 
TCACTGCTCT CCTCACTCCA GGACAATGGA TTGACAGATG TCATGCTGCA TGCACTGCTT 
ATCAAAGATG TTCCTGCTAC CCGTGAAGTC CTTGGCTCCC TCCCAAATGT ATTCAGTGCA 
CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT TTTGTTCAGT GTCAGCCTTT TGAACGCCTC 
TTCAAAGTTC TTCTGTCTCC AGATTACCTC CCAGCCATGC GGAGGAGGAG AAGTTCTGAT 
CCCCTTGGGG ATACTGCATC CAACCTGGGG AGTGCTGTCG ATGAGCTCAT GAGACATCAG 
CCCACCCTTA AAACAGATGC AACGACTGCC ATCATCAAGT TACTTGAAGA AATCTGTAAT 
CTTGGAAGGG ACCCCAAATA CATCTGTCAG AAGCCATCAA TCCAGAAGGC AGATGGCACT 
GCCACTGCTC CTCCCCCAAG GTCTAATCAT GCCGCAGAAG AAGCCTCTAG TGAGGATGAG 
GAGGAAGAGG AAGTACAGGC CATGCAGAGC TTTAATTCTA CCCAGCAAAA TGAAACTGAG 
CCTAATCAGC AGGTTGTTGG TACAGAGGAA CGTATTCCTA TTCCCCTCAT GGATTACATC 
CTTAATGTGA TGAAATTTGT GGAATCTATT CTGAGCAACA ATACAACAGA TGACCACTGC 
CAGGAATTTG TGAATCAGAA AGGACTGTTG CCTTTGGTTA CCATTTTGGG TCTTCCCAAT 
CTGCCCATTG ACTTTCCCAC ATCTGCTGCC TGTCAGGCTG TTGCAGGTGT CTGCAAATCC 
ATATTGACAC TGTCACATGA ACCCAAAGTC CTTCAAGAGG GTCTCCTTCA GTTGGACTCC 
ATCCTCTCCT CCCTGGAGCC CTTACACCGC CCCATTGAAT CCCCTGGGGG CTCAGTGTTG 
TTGCGAGAAC TGGCTTGCGC AGGCAATGTT GCTGATGCTA CCCTCTCAGC CCAGGCCACA 
CCTCTGCTGC ATGCACTCAC TGCTGCCCAT GCCTACATCA TGATGTTTGT TCATACTTGC 
AGAGTTGGAC AGAGTGAAAT TCGTTCCATC TCCGTAAACC AGTGGGGCTC TCAATTGGGT 
CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA TACTGTTCCC TGGTGTGGGA AAGCACTGTC 
CTCCTCTCTC TGTGTACCCC AAACAGCCTA CCATCTGGGT GTGAATTTGG CCAGGCAGAT 
ATGCAGAAAC TGGTTCCAAA GGATGAGAAG GCAGGTACGA CCCAGGGCGG AAAAAGATCA 
GATGGGGAAC AGGATGGAGC AGCTGGAAGT ATGGATGCTT CTACCCAGGG CTTATTAGAA 
GGCATTGGGC TAGATGGTGA CACATTGGCT CCCATGGAGA CAGATGAACC TACTGCTTCA 
GACTCTAAGG GCAAATCTAA AATCACACCA GCAATGGCTG CCAGAATTAA GCAAATCAAG 
CCTTTGTTAT CAGCTTCCTC CAGATTAGGC CGAGCACTTG CTGAGCTATT TGGACTTCTT 
GTTAAACTTT GTGTGGGATC TCCTGTCCGC CAGAGAAGGA GCCATCATGC TGCCAGCACC 
ACTACAGCAC CGACACCTGC CGCGCGATCA ACAGCCTCAG CTCTCACTAA GCTCTTGACT 
AAGGGGTTAT CTTGGCAGCC CCCACCATAT ACACCTACTC CCCGATTCAG GCTGACATTC 
TTCATCTGTT CAGTTGGTTT CACATCCCCA ATGCTGTTTG ATGAGAGGAA GTATCCCTAC 
CACCTCATGC TGCAAAAATT TCTCTGCTCC GGAGGCCACA ATGCTCTTTT TGAAACTTTC 
AACTGGGCTC TGTCCATGGG AGGTAAAGTT CCTGTTTCTG AGGGATTGGA ACACTCAGAC 
TTGCCTGATG GCACAGGAGA ATTCCTAGAT GCCTGGCTTA TGCTGGTGGA GAAGATGGTG 
AATCCCACCA CGGTGCTTGA ATCTCCACAT TCGCTGCCTG CCAAATTGCC TGGAGGTGTC 
CAGAACTTTC CCCAGTTCAG TGCACTGCGC TTCCTTGTGG TAACTCAGAA AGCAGCCTTT 
ACTTGCATCA AAAACTTATG GAACCGGAAA CCCCTGAAGG TATATGGTGG ACGAATGGCT 
GAATCGATGC TGGCCATTCT ATGCCACATC CTCCGAGGAG AACCTGTGAT TCGAGAGAGA 
CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA GAAGAGGATA CAGGGCAAGA GGAAGGTGGC 
TCCCGCCGGG AACCTCAAGT CAACCAGCAA CAACTGCAAC AGCTCATGGA CATGGGCTTC 
ACAAGGGAAC ATGCAATGGA GGCACTGTTG AACACCAGCA CCATGGAGCA GGCCACAGAG 
TACCTTTTAA CCCACCCTCC TCCAATCATG GGAGGAGTTG TTCGGGATCT CAGCATGTCT 
GAAGAGGACC AGATGATGAG AGCAATTGCT ATGTCTCTGG GACAGGATAT TCCAATGGAT 
CAAAGGGCAG AGTCACCTGA GGAAGTTGCT TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT 
CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA TGTCTAGAGA AGTTCCAGGA TGCTGACCCG 
TTGGAACAAG ATGAGCTCCA CACTTTCACA GATACTATGT TGCCAGGCTG CTTCCACCTT 
CTTGATGAGC TGCCAGACAC AGTATACCGT GTGTGTGACC TGATCATGAC AGCAATCAAA 
CGTAATGGAG CAGATTATCG TGACATGATT CTGAAGCAAG TAGTCAATCA GGTGTGGGAA 
GCTGCTGATG TATTGATCAA AGCTGCTCTT CCCCTGACAA CAAGTGACAC AAAAACCGTG 
TCAGAGTGGA TAAGTCAGAT GGCCACACTG CCCCAGGCCT CCAATTTGGC TACTAGAATC 
TTGCTTTTAA CGCTACTTTT TGAGGAGTTG AAGCTACCTT GTGCTTGGGT GGTTGAATCA 
AGTGGCATCC TTAATGTCCT AATCAAACTC TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA 
GCCAAGGAGC AGAAGGAAGT CCAGACCCCA AAGTGGATCA CACCAGTGTT GCTCCTGATT 
GATTTCTATG AAAAGACAGC CATCTCCTCA AAAAGGAGAG CCCAGATGAC TAAGTACCTG 
CAATCCAACA GCAACAACTG GCGCTGGTTT GATGATCGCT CTGGGCGTTG GTGTAGTTAC 
AGTGCAAGCA ACAATAGCAC TATTGATTCT GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA 
TTCACTGCAG GCCGAAGAAG ATACACGGTC CAATTCACTA CAATGGTGCA GGTTAATGAG 
GAAACAGGGA ACCGACGCCC TGTGATGCTG ACTCTCCTCA GGGTACCTCG GCTGAATAAA 
AATTCAAAAA ACAGCAATGG ACAGGAACTA GAGAAGACGC TGGAAGAAAG CAAAGAAATG 
GATATCAAAC GTAAAGAAAA TAAAGGCAAT GATACCCCTT TGGCCCTAGA GAGTACAAAC 
ACTGAAAAGG AGACAAGCCT GGAGGAAACA AAAATCGGGG AGATCCTGAT CCAGGGCTTG 
ACAGAAGATA TGGTGACTGT TTTAATCCGG GCCTGCGTGA GCATGCTGGG AGTCCCTGTG 
GACCCAGATA CTTTGCATGC CACCCTTCGT CTCTGTCTGA GGCTCACCCG GGACCACAAA 
TATGCCATGA TGTTTGCAGA ACTGAAGAGT ACCCGCATGA TCTTGAATTT GACCCAGAGC 
TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC ACCCTTCTCT TAAGACACAT CATTGAGGAC 
CCCTGTACCC TTCGTCATAC CATGGAAAAG GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT 
GGTAGCACTA CCTCTGGTGT TGTGTCTGGC AGCCTCGGCT CTCGGGAGAT CAACTACATC 
CTTCGTGTCC TTGGGCCAGC CGCATGCCGC AATCCAGACA TATTCACAGA AGTGGCCAAC 
TGCTGTATCC GCATCGCCCT TCCTGCCCCT CGAGGCTCAG GAACTGCTTC AGATGATGAA 
TTTGAGAATC TTAGAATTAA AGGCCCTAAT GCTGTACAGC TGGTGAAGAC CACCCCTTTG 
AAGCCCTCAC CTCTGCCTGT CATCCCTGAT ACTATCAAGG AAGTGATCTA TGATATGCTG 
AATGCTCTGG CTGCATACCA TGCTCCAGAG GAAGCAGATA AATCTGATCC TAAACCTGGG 
GTTATGACCC AAGAGGTTGG CCAGCTCCTG CAAGACATGG GTGATGATGT ATACCAGCAG 
TACCGGTCAC TTACGCGTCA GAGCAGTGAC TTTGATACGC AGTCAGGTTT TTCCATTAAT 
AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC ACTGAGACTT CCGCATCTGG GACCTCCCAA 
GGAGAGGCTT CAACTCCAGA GGAGTCTCGA GATGGGAAGA AAGATAAAGA AGGGGACCGG 
GCCTCTGAGG AAGGCAAACA GAAAGGCAAG GGCAGCAAAC CTTTAATGCC TACCTCCACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCC 
AACTACAGCT ACACTGTGGG CCAGTCTGAA CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTTCTGG ACCACCTGCT CCCACATACC CAGAATGCAG AAGACAAGGA CACCCCTGCC 
TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT GCTGCAGGGA GTGGCACAGA TGCCCAGGTG 
GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT GGACGGGCAC TGGCTATGGC TGAGAGTACA 
GAGAAACATG CCAGGCTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAGTCCTGC 
CCCTCCACCT CCAGCTTCTA CAGCAGTGCC ACAGCGAAGA CCCAGCACAA TGGCATGAAC 
AACATCATTC GGCTTTTCCT GAAGAAGGGA CTGGTTAATG ACCTGGCCAG AGTACCTCAC 
AGCTTAGACC TGTCCAGTCC CAACATGGCC AACACAGTCA ATGCTGCTCT GAAGCCTTTG 
GAAACACTTT CCCGGATTGT GAACCAGCCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCCCAA GGAGCCTCTC AAGATTCCAG TAGCAACCAG 
CAGGACCCAG GCGAGCCTGG GGAAGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCTGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAGAATGA GCTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AGCACACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAGG GAGATGAAGA CGATGACGAC GATGGCTCTG AGATGGAATT GGATGAGGAT 
TATCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATCCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCATCC ACTGATGGTG CGCCATGCAG ACCACAGTTC TCTGACACTG 
GGCAGTGGCT CTTCAACAAC TCGTCTCACC CAGGGCATCG GGCGCAGTCA GAGGACCCTA 
AGGCAGCTGA CGGCCAATAC TGGCCACACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCCAACCCTC CTCTTATACT GCAGAGGTTG CTTGGTCCCT CAGCTGCTGC TGACATCCTT 
CAGCTGAGCA GCAGCCTTCC CCTACAAAGC CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC 
GATGACGTCC ACATCATCGC CCGTTCTGAT GATGAGCTGC TGGATGACTT TTTCCATGAT 
CAGAGCACAG CTACCAGCCA AGCAGGAACC CTGTCCAGCA TCCCCACAGC CCTGACCCGC 
TGGACAGAAG AATGCAAAGT TCTCGATGCT GAGAGCATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGTCCA TTGTCAATCA CCTGGAATTC CTGAGGGATG AGGAGCTGGA AGAAAGGCGA 
GAGAAGCGCA GGAAACAACT GGCTGAGGAA GAAACAAAGA TAACTGATAA AGGCAAAGAA 
GATAAGGAGA ACAGGGATCA GAGTGCACAG TGTACTGCAT CTAAGTCAAA TGACTCCACT 
GAACAGAATC TCTCAGATGG GACGCCTATG CCTGACAGCT ACCCAACAAC CCCATCTTCA 
ACTGATGCAG CTACATCTGA GTCCAAGGAG ACCCTTGGCA CTCTGCAATC CTCACAACAG 
CAACCAACAC TCCCAACCCC ACCAGCTTTG GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA 
GCTGGAGAAG GGGGCAGCTC TACACAGCTA TTGATGCCTG TAGAGCCAGA GGAATTGGGT 
CCCACAAGGC CAAGTGGGGA AGCAGAAACA ACTCAGATGG AGTTATCCCC AGCTCCCACT 
ATAACCTCAC TTTCCCCAGA GAGAGCTGAG GATTCTGATG CACTGACGGC TGTCAGCAGT 
CAGCTAGAAG GCTCTCCTAT GGATACAAGC AGCCTGGCTT CCTGTACCTT AGAGGAGGCT 
GTGGGTGACA CTTCAGCAGC TGGCAGTTCT GAGCAGCCCA GAGCAGGCAG CTCCACTCCT 
GGGGATGCCC CACCAGCTGT GGCGGAAGTG CAAGGCAGGA GTGATGGGTC AGGGGAATCT 
GCCCAGCCAC CTGAGGACAG CTCCCCACCT GCATCCTCTG AGAGCTCTTC CACCAGAGAT 
TCTGCCGTGG CCATTTCTGG AGCAGATTCC CGAGGAATCC TAGAAGAGCC GTTGCCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG GGTATCAGTC TCCCTGAAGG TGTGGACCCC 
TCTTTTCTGG CTGCCCTGCC TGATGACATC CGTCGGGAAG TTCTACAGAA CCAGCTAGGC 
ATTCGTCCAC CAACCCGGAC TGCCCCCTCC ACAAATAGCT CAGCGCCTGC AGTGGTGGGG 
AATCCTGGTG TGACTGAAGT GAGCCCTGAG TTTCTGGCTG CCCTGCCTCC AGCCATTCAG 
GAGGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884688.1
CDS	398..13792
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029199.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029199.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTTTGTT TCTCCTTTTG CAGTGTATTC CAATGCCTTG 
CAGGAATCAG CAAACAGTAT CTTGTATAAT GGCTTGATAG AGGAGTTGGT AGATGTCCTT 
CAGATAACGG ATAAGCAGCT TATGGAGATT AAAGCAGCTT CTTTACGAAC ATTAACATCA 
ATTGTCCACT TGGAGAGAAC TCCCAAACTC AGCAGTATTA TTGACTGTAC TGGAACTGCC 
TCCTACCATG GATTTTTGCC AGTGCTTGTA AGGAACTGTA TCCAGGCCAT GATTGATCCT 
TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG 
GCCAGCTACG ATGCTGGTGG TGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG 
AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCCGTC 
AGAGTGGTTG ACCTTATCAC CAACCTGGAT ATGGCAGCTT TTCAATCCCA TAGTGGACTT 
TCTATCTTCA TTTATAGACT TGAGCATGAA GTAGATTTGT GCCGAAAAGA ATGTCCGTTT 
GTGATCAAGC CAAAGATCCA GAGACCCAAT ACTACACAAG AAGGAGAGGA AATGGAAACT 
GATATGGATG TTGCAGATGT GGCTATGGAA AGCAGTCCAG GCTCATCCAT CTCTATGGAG 
CACAGGCTGG ATGTTGAATT AAGGGCATCA GGTTCCAGCA GCAGCACTAA CATCTCTTCT 
GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC GGCACCGGCC CTGGCCCCGG CCCCGGCCCC 
GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT 
GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT ATTCCACAAC GAGCAGCACT TCTGAAATCC 
ATGTTGAATT TCCTCAAGAA GGCCATCCAA GACCCTGCTT TCTCAGATGG CATACGACAT 
GTGATGGATG GTTCTCTGCC TACCTCCCTG AAACACATCA TCAGCAATGC AGAATACTAT 
GGCCCATCAC TCTTCCTCCT AGCTACTGAA GTGGTGACTG TGTTTGTATT TCAAGAACCA 
TCACTGCTCT CCTCACTCCA GGACAATGGA TTGACAGATG TCATGCTGCA TGCACTGCTT 
ATCAAAGATG TTCCTGCTAC CCGTGAAGTC CTTGGCTCCC TCCCAAATGT ATTCAGTGCA 
CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT TTTGTTCAGT GTCAGCCTTT TGAACGCCTC 
TTCAAAGTTC TTCTGTCTCC AGATTACCTC CCAGCCATGC GGAGGAGGAG AAGTTCTGAT 
CCCCTTGGGG ATACTGCATC CAACCTGGGG AGTGCTGTCG ATGAGCTCAT GAGACATCAG 
CCCACCCTTA AAACAGATGC AACGACTGCC ATCATCAAGT TACTTGAAGA AATCTGTAAT 
CTTGGAAGGG ACCCCAAATA CATCTGTCAG AAGCCATCAA TCCAGAAGGC AGATGGCACT 
GCCACTGCTC CTCCCCCAAG GTCTAATCAT GCCGCAGAAG AAGCCTCTAG TGAGGATGAG 
GAGGAAGAGG AAGTACAGGC CATGCAGAGC TTTAATTCTA CCCAGCAAAA TGAAACTGAG 
CCTAATCAGC AGGTTGTTGG TACAGAGGAA CGTATTCCTA TTCCCCTCAT GGATTACATC 
CTTAATGTGA TGAAATTTGT GGAATCTATT CTGAGCAACA ATACAACAGA TGACCACTGC 
CAGGAATTTG TGAATCAGAA AGGACTGTTG CCTTTGGTTA CCATTTTGGG TCTTCCCAAT 
CTGCCCATTG ACTTTCCCAC ATCTGCTGCC TGTCAGGCTG TTGCAGGTGT CTGCAAATCC 
ATATTGACAC TGTCACATGA ACCCAAAGTC CTTCAAGAGG GTCTCCTTCA GTTGGACTCC 
ATCCTCTCCT CCCTGGAGCC CTTACACCGC CCCATTGAAT CCCCTGGGGG CTCAGTGTTG 
TTGCGAGAAC TGGCTTGCGC AGGCAATGTT GCTGATGCTA CCCTCTCAGC CCAGGCCACA 
CCTCTGCTGC ATGCACTCAC TGCTGCCCAT GCCTACATCA TGATGTTTGT TCATACTTGC 
AGAGTTGGAC AGAGTGAAAT TCGTTCCATC TCCGTAAACC AGTGGGGCTC TCAATTGGGT 
CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA TACTGTTCCC TGGTGTGGGA AAGCACTGTC 
CTCCTCTCTC TGTGTACCCC AAACAGCCTA CCATCTGGGT GTGAATTTGG CCAGGCAGAT 
ATGCAGAAAC TGGTTCCAAA GGATGAGAAG GCAGGTACGA CCCAGGGCGG AAAAAGATCA 
GATGGGGAAC AGGATGGAGC AGCTGGAAGT ATGGATGCTT CTACCCAGGG CTTATTAGAA 
GGCATTGGGC TAGATGGTGA CACATTGGCT CCCATGGAGA CAGATGAACC TACTGCTTCA 
GACTCTAAGG GCAAATCTAA AATCACACCA GCAATGGCTG CCAGAATTAA GCAAATCAAG 
CCTTTGTTAT CAGCTTCCTC CAGATTAGGC CGAGCACTTG CTGAGCTATT TGGACTTCTT 
GTTAAACTTT GTGTGGGATC TCCTGTCCGC CAGAGAAGGA GCCATCATGC TGCCAGCACC 
ACTACAGCAC CGACACCTGC CGCGCGATCA ACAGCCTCAG CTCTCACTAA GCTCTTGACT 
AAGGGGTTAT CTTGGCAGCC CCCACCATAT ACACCTACTC CCCGATTCAG GCTGACATTC 
TTCATCTGTT CAGTTGGTTT CACATCCCCA ATGCTGTTTG ATGAGAGGAA GTATCCCTAC 
CACCTCATGC TGCAAAAATT TCTCTGCTCC GGAGGCCACA ATGCTCTTTT TGAAACTTTC 
AACTGGGCTC TGTCCATGGG AGGTAAAGTT CCTGTTTCTG AGGGATTGGA ACACTCAGAC 
TTGCCTGATG GCACAGGAGA ATTCCTAGAT GCCTGGCTTA TGCTGGTGGA GAAGATGGTG 
AATCCCACCA CGGTGCTTGA ATCTCCACAT TCGCTGCCTG CCAAATTGCC TGGAGGTGTC 
CAGAACTTTC CCCAGTTCAG TGCACTGCGC TTCCTTGTGG TAACTCAGAA AGCAGCCTTT 
ACTTGCATCA AAAACTTATG GAACCGGAAA CCCCTGAAGG TATATGGTGG ACGAATGGCT 
GAATCGATGC TGGCCATTCT ATGCCACATC CTCCGAGGAG AACCTGTGAT TCGAGAGAGA 
CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA GAAGAGGATA CAGGGCAAGA GGAAGGTGGC 
TCCCGCCGGG AACCTCAAGT CAACCAGCAA CAACTGCAAC AGCTCATGGA CATGGGCTTC 
ACAAGGGAAC ATGCAATGGA GGCACTGTTG AACACCAGCA CCATGGAGCA GGCCACAGAG 
TACCTTTTAA CCCACCCTCC TCCAATCATG GGAGGAGTTG TTCGGGATCT CAGCATGTCT 
GAAGAGGACC AGATGATGAG AGCAATTGCT ATGTCTCTGG GACAGGATAT TCCAATGGAT 
CAAAGGGCAG AGTCACCTGA GGAAGTTGCT TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT 
CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA TGTCTAGAGA AGTTCCAGGA TGCTGACCCG 
TTGGAACAAG ATGAGCTCCA CACTTTCACA GATACTATGT TGCCAGGCTG CTTCCACCTT 
CTTGATGAGC TGCCAGACAC AGTATACCGT GTGTGTGACC TGATCATGAC AGCAATCAAA 
CGTAATGGAG CAGATTATCG TGACATGATT CTGAAGCAAG TAGTCAATCA GGTGTGGGAA 
GCTGCTGATG TATTGATCAA AGCTGCTCTT CCCCTGACAA CAAGTGACAC AAAAACCGTG 
TCAGAGTGGA TAAGTCAGAT GGCCACACTG CCCCAGGCCT CCAATTTGGC TACTAGAATC 
TTGCTTTTAA CGCTACTTTT TGAGGAGTTG AAGCTACCTT GTGCTTGGGT GGTTGAATCA 
AGTGGCATCC TTAATGTCCT AATCAAACTC TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA 
GCCAAGGAGC AGAAGGAAGT CCAGACCCCA AAGTGGATCA CACCAGTGTT GCTCCTGATT 
GATTTCTATG AAAAGACAGC CATCTCCTCA AAAAGGAGAG CCCAGATGAC TAAGTACCTG 
CAATCCAACA GCAACAACTG GCGCTGGTTT GATGATCGCT CTGGGCGTTG GTGTAGTTAC 
AGTGCAAGCA ACAATAGCAC TATTGATTCT GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA 
TTCACTGCAG GCCGAAGAAG ATACACGGTC CAATTCACTA CAATGGTGCA GGTTAATGAG 
GAAACAGGGA ACCGACGCCC TGTGATGCTG ACTCTCCTCA GGGTACCTCG GCTGAATAAA 
AATTCAAAAA ACAGCAATGG ACAGGAACTA GAGAAGACGC TGGAAGAAAG CAAAGAAATG 
GATATCAAAC GTAAAGAAAA TAAAGGCAAT GATACCCCTT TGGCCCTAGA GAGTACAAAC 
ACTGAAAAGG AGACAAGCCT GGAGGAAACA AAAATCGGGG AGATCCTGAT CCAGGGCTTG 
ACAGAAGATA TGGTGACTGT TTTAATCCGG GCCTGCGTGA GCATGCTGGG AGTCCCTGTG 
GACCCAGATA CTTTGCATGC CACCCTTCGT CTCTGTCTGA GGCTCACCCG GGACCACAAA 
TATGCCATGA TGTTTGCAGA ACTGAAGAGT ACCCGCATGA TCTTGAATTT GACCCAGAGC 
TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC ACCCTTCTCT TAAGACACAT CATTGAGGAC 
CCCTGTACCC TTCGTCATAC CATGGAAAAG GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT 
GGTAGCACTA CCTCTGGTGT TGTGTCTGGC AGCCTCGGCT CTCGGGAGAT CAACTACATC 
CTTCGTGTCC TTGGGCCAGC CGCATGCCGC AATCCAGACA TATTCACAGA AGTGGCCAAC 
TGCTGTATCC GCATCGCCCT TCCTGCCCCT CGAGGCTCAG GAACTGCTTC AGATGATGAA 
TTTGAGAATC TTAGAATTAA AGGCCCTAAT GCTGTACAGC TGGTGAAGAC CACCCCTTTG 
AAGCCCTCAC CTCTGCCTGT CATCCCTGAT ACTATCAAGG AAGTGATCTA TGATATGCTG 
AATGCTCTGG CTGCATACCA TGCTCCAGAG GAAGCAGATA AATCTGATCC TAAACCTGGG 
GTTATGACCC AAGAGGTTGG CCAGCTCCTG CAAGACATGG GTGATGATGT ATACCAGCAG 
TACCGGTCAC TTACGCGTCA GAGCAGTGAC TTTGATACGC AGTCAGGTTT TTCCATTAAT 
AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC ACTGAGACTT CCGCATCTGG GACCTCCCAA 
GGAGAGGCTT CAACTCCAGA GGAGTCTCGA GATGGGAAGA AAGATAAAGA AGGGGACCGG 
GCCTCTGAGG AAGGCAAACA GAAAGGCAAG GGCAGCAAAC CTTTAATGCC TACCTCCACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCC 
AACTACAGCT ACACTGTGGG CCAGTCTGAA CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTTCTGG ACCACCTGCT CCCACATACC CAGAATGCAG AAGACAAGGA CACCCCTGCC 
TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT GCTGCAGGGA GTGGCACAGA TGCCCAGGTG 
GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT GGACGGGCAC TGGCTATGGC TGAGAGTACA 
GAGAAACATG CCAGGCTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAGTCCTGC 
CCCTCCACCT CCAGCTTCTA CAGCAGTGCC ACAGCGAAGA CCCAGCACAA TGGCATGAAC 
AACATCATTC GGCTTTTCCT GAAGAAGGGA CTGGTTAATG ACCTGGCCAG AGTACCTCAC 
AGCTTAGACC TGTCCAGTCC CAACATGGCC AACACAGTCA ATGCTGCTCT GAAGCCTTTG 
GAAACACTTT CCCGGATTGT GAACCAGCCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCCCAA GGAGCCTCTC AAGATTCCAG TAGCAACCAG 
CAGGACCCAG GCGAGCCTGG GGAAGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCTGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAGAATGA GCTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AGCACACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAGG GAGATGAAGA CGATGACGAC GATGGCTCTG AGATGGAATT GGATGAGGAT 
TATCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATCCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCATCC ACTGATGGTG CGCCATGCAG ACCACAGTTC TCTGACACTG 
GGCAGTGGCT CTTCAACAAC TCGTCTCACC CAGGGCATCG GGCGCAGTCA GAGGACCCTA 
AGGCAGCTGA CGGCCAATAC TGGCCACACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCCAACCCTC CTCTTATACT GCAGAGGTTG CTTGGTCCCT CAGCTGCTGC TGACATCCTT 
CAGCTGAGCA GCAGCCTTCC CCTACAAAGC CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC 
GATGACGTCC ACATCATCGC CCGTTCTGAT GATGAGCTGC TGGATGACTT TTTCCATGAT 
CAGAGCACAG CTACCAGCCA AGCAGGAACC CTGTCCAGCA TCCCCACAGC CCTGACCCGC 
TGGACAGAAG AATGCAAAGT TCTCGATGCT GAGAGCATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGTCCA TTGTCAATCA CCTGGAATTC CTGAGGGATG AGGAGCTGGA AGAAAGGCGA 
GAGAAGCGCA GGAAACAACT GGCTGAGGAA GAAACAAAGA TAACTGATAA AGGCAAAGAA 
GATAAGGAGA ACAGGGATCA GAGTGCACAG TGTACTGCAT CTAAGTCAAA TGACTCCACT 
GAACAGAATC TCTCAGATGG GACGCCTATG CCTGACAGCT ACCCAACAAC CCCATCTTCA 
ACTGATGCAG CTACATCTGA GTCCAAGGAG ACCCTTGGCA CTCTGCAATC CTCACAACAG 
CAACCAACAC TCCCAACCCC ACCAGCTTTG GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA 
GCTGGAGAAG GGGGCAGCTC TACACAGCTA TTGATGCCTG TAGAGCCAGA GGAATTGGGT 
CCCACAAGGC CAAGTGGGGA AGCAGAAACA ACTCAGATGG AGTTATCCCC AGCTCCCACT 
ATAACCTCAC TTTCCCCAGA GAGAGCTGAG GATTCTGATG CACTGACGGC TGTCAGCAGT 
CAGCTAGAAG GCTCTCCTAT GGATACAAGC AGCCTGGCTT CCTGTACCTT AGAGGAGGCT 
GTGGGTGACA CTTCAGCAGC TGGCAGTTCT GAGCAGCCCA GAGCAGGCAG CTCCACTCCT 
GGGGATGCCC CACCAGCTGT GGCGGAAGTG CAAGGCAGGA GTGATGGGTC AGGGGAATCT 
GCCCAGCCAC CTGAGGACAG CTCCCCACCT GCATCCTCTG AGAGCTCTTC CACCAGAGAT 
TCTGCCGTGG CCATTTCTGG AGCAGATTCC CGAGGAATCC TAGAAGAGCC GTTGCCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG GGTATCAGTC TCCCTGAAGG TGTGGACCCC 
TCTTTTCTGG CTGCCCTGCC TGATGACATC CGTCGGGAAG TTCTACAGAA CCAGCTAGGC 
ATTCGTCCAC CAACCCGGAC TGCCCCCTCC ACAAATAGCT CAGCGCCTGC AGTGGTGGGG 
AATCCTGGTG TGACTGAAGT GAGCCCTGAG TTTCTGGCTG CCCTGCCTCC AGCCATTCAG 
GAGGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029201.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884690.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X11, mRNA.

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CloneID	OHu99119
Clone ID Related Accession (Same CDS sequence)	XM_017029199.1 , XM_017029201.1 , XM_017029203.1 , XM_017029200.1 , XM_017029202.1 , XM_017029198.1 , XM_017029198.2
Accession Version	XM_017029201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTTTGTT TCTCCTTTTG CAGTGTATTC CAATGCCTTG 
CAGGAATCAG CAAACAGTAT CTTGTATAAT GGCTTGATAG AGGAGTTGGT AGATGTCCTT 
CAGATAACGG ATAAGCAGCT TATGGAGATT AAAGCAGCTT CTTTACGAAC ATTAACATCA 
ATTGTCCACT TGGAGAGAAC TCCCAAACTC AGCAGTATTA TTGACTGTAC TGGAACTGCC 
TCCTACCATG GATTTTTGCC AGTGCTTGTA AGGAACTGTA TCCAGGCCAT GATTGATCCT 
TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG 
GCCAGCTACG ATGCTGGTGG TGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG 
AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCCGTC 
AGAGTGGTTG ACCTTATCAC CAACCTGGAT ATGGCAGCTT TTCAATCCCA TAGTGGACTT 
TCTATCTTCA TTTATAGACT TGAGCATGAA GTAGATTTGT GCCGAAAAGA ATGTCCGTTT 
GTGATCAAGC CAAAGATCCA GAGACCCAAT ACTACACAAG AAGGAGAGGA AATGGAAACT 
GATATGGATG TTGCAGATGT GGCTATGGAA AGCAGTCCAG GCTCATCCAT CTCTATGGAG 
CACAGGCTGG ATGTTGAATT AAGGGCATCA GGTTCCAGCA GCAGCACTAA CATCTCTTCT 
GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC GGCACCGGCC CTGGCCCCGG CCCCGGCCCC 
GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT 
GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT ATTCCACAAC GAGCAGCACT TCTGAAATCC 
ATGTTGAATT TCCTCAAGAA GGCCATCCAA GACCCTGCTT TCTCAGATGG CATACGACAT 
GTGATGGATG GTTCTCTGCC TACCTCCCTG AAACACATCA TCAGCAATGC AGAATACTAT 
GGCCCATCAC TCTTCCTCCT AGCTACTGAA GTGGTGACTG TGTTTGTATT TCAAGAACCA 
TCACTGCTCT CCTCACTCCA GGACAATGGA TTGACAGATG TCATGCTGCA TGCACTGCTT 
ATCAAAGATG TTCCTGCTAC CCGTGAAGTC CTTGGCTCCC TCCCAAATGT ATTCAGTGCA 
CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT TTTGTTCAGT GTCAGCCTTT TGAACGCCTC 
TTCAAAGTTC TTCTGTCTCC AGATTACCTC CCAGCCATGC GGAGGAGGAG AAGTTCTGAT 
CCCCTTGGGG ATACTGCATC CAACCTGGGG AGTGCTGTCG ATGAGCTCAT GAGACATCAG 
CCCACCCTTA AAACAGATGC AACGACTGCC ATCATCAAGT TACTTGAAGA AATCTGTAAT 
CTTGGAAGGG ACCCCAAATA CATCTGTCAG AAGCCATCAA TCCAGAAGGC AGATGGCACT 
GCCACTGCTC CTCCCCCAAG GTCTAATCAT GCCGCAGAAG AAGCCTCTAG TGAGGATGAG 
GAGGAAGAGG AAGTACAGGC CATGCAGAGC TTTAATTCTA CCCAGCAAAA TGAAACTGAG 
CCTAATCAGC AGGTTGTTGG TACAGAGGAA CGTATTCCTA TTCCCCTCAT GGATTACATC 
CTTAATGTGA TGAAATTTGT GGAATCTATT CTGAGCAACA ATACAACAGA TGACCACTGC 
CAGGAATTTG TGAATCAGAA AGGACTGTTG CCTTTGGTTA CCATTTTGGG TCTTCCCAAT 
CTGCCCATTG ACTTTCCCAC ATCTGCTGCC TGTCAGGCTG TTGCAGGTGT CTGCAAATCC 
ATATTGACAC TGTCACATGA ACCCAAAGTC CTTCAAGAGG GTCTCCTTCA GTTGGACTCC 
ATCCTCTCCT CCCTGGAGCC CTTACACCGC CCCATTGAAT CCCCTGGGGG CTCAGTGTTG 
TTGCGAGAAC TGGCTTGCGC AGGCAATGTT GCTGATGCTA CCCTCTCAGC CCAGGCCACA 
CCTCTGCTGC ATGCACTCAC TGCTGCCCAT GCCTACATCA TGATGTTTGT TCATACTTGC 
AGAGTTGGAC AGAGTGAAAT TCGTTCCATC TCCGTAAACC AGTGGGGCTC TCAATTGGGT 
CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA TACTGTTCCC TGGTGTGGGA AAGCACTGTC 
CTCCTCTCTC TGTGTACCCC AAACAGCCTA CCATCTGGGT GTGAATTTGG CCAGGCAGAT 
ATGCAGAAAC TGGTTCCAAA GGATGAGAAG GCAGGTACGA CCCAGGGCGG AAAAAGATCA 
GATGGGGAAC AGGATGGAGC AGCTGGAAGT ATGGATGCTT CTACCCAGGG CTTATTAGAA 
GGCATTGGGC TAGATGGTGA CACATTGGCT CCCATGGAGA CAGATGAACC TACTGCTTCA 
GACTCTAAGG GCAAATCTAA AATCACACCA GCAATGGCTG CCAGAATTAA GCAAATCAAG 
CCTTTGTTAT CAGCTTCCTC CAGATTAGGC CGAGCACTTG CTGAGCTATT TGGACTTCTT 
GTTAAACTTT GTGTGGGATC TCCTGTCCGC CAGAGAAGGA GCCATCATGC TGCCAGCACC 
ACTACAGCAC CGACACCTGC CGCGCGATCA ACAGCCTCAG CTCTCACTAA GCTCTTGACT 
AAGGGGTTAT CTTGGCAGCC CCCACCATAT ACACCTACTC CCCGATTCAG GCTGACATTC 
TTCATCTGTT CAGTTGGTTT CACATCCCCA ATGCTGTTTG ATGAGAGGAA GTATCCCTAC 
CACCTCATGC TGCAAAAATT TCTCTGCTCC GGAGGCCACA ATGCTCTTTT TGAAACTTTC 
AACTGGGCTC TGTCCATGGG AGGTAAAGTT CCTGTTTCTG AGGGATTGGA ACACTCAGAC 
TTGCCTGATG GCACAGGAGA ATTCCTAGAT GCCTGGCTTA TGCTGGTGGA GAAGATGGTG 
AATCCCACCA CGGTGCTTGA ATCTCCACAT TCGCTGCCTG CCAAATTGCC TGGAGGTGTC 
CAGAACTTTC CCCAGTTCAG TGCACTGCGC TTCCTTGTGG TAACTCAGAA AGCAGCCTTT 
ACTTGCATCA AAAACTTATG GAACCGGAAA CCCCTGAAGG TATATGGTGG ACGAATGGCT 
GAATCGATGC TGGCCATTCT ATGCCACATC CTCCGAGGAG AACCTGTGAT TCGAGAGAGA 
CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA GAAGAGGATA CAGGGCAAGA GGAAGGTGGC 
TCCCGCCGGG AACCTCAAGT CAACCAGCAA CAACTGCAAC AGCTCATGGA CATGGGCTTC 
ACAAGGGAAC ATGCAATGGA GGCACTGTTG AACACCAGCA CCATGGAGCA GGCCACAGAG 
TACCTTTTAA CCCACCCTCC TCCAATCATG GGAGGAGTTG TTCGGGATCT CAGCATGTCT 
GAAGAGGACC AGATGATGAG AGCAATTGCT ATGTCTCTGG GACAGGATAT TCCAATGGAT 
CAAAGGGCAG AGTCACCTGA GGAAGTTGCT TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT 
CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA TGTCTAGAGA AGTTCCAGGA TGCTGACCCG 
TTGGAACAAG ATGAGCTCCA CACTTTCACA GATACTATGT TGCCAGGCTG CTTCCACCTT 
CTTGATGAGC TGCCAGACAC AGTATACCGT GTGTGTGACC TGATCATGAC AGCAATCAAA 
CGTAATGGAG CAGATTATCG TGACATGATT CTGAAGCAAG TAGTCAATCA GGTGTGGGAA 
GCTGCTGATG TATTGATCAA AGCTGCTCTT CCCCTGACAA CAAGTGACAC AAAAACCGTG 
TCAGAGTGGA TAAGTCAGAT GGCCACACTG CCCCAGGCCT CCAATTTGGC TACTAGAATC 
TTGCTTTTAA CGCTACTTTT TGAGGAGTTG AAGCTACCTT GTGCTTGGGT GGTTGAATCA 
AGTGGCATCC TTAATGTCCT AATCAAACTC TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA 
GCCAAGGAGC AGAAGGAAGT CCAGACCCCA AAGTGGATCA CACCAGTGTT GCTCCTGATT 
GATTTCTATG AAAAGACAGC CATCTCCTCA AAAAGGAGAG CCCAGATGAC TAAGTACCTG 
CAATCCAACA GCAACAACTG GCGCTGGTTT GATGATCGCT CTGGGCGTTG GTGTAGTTAC 
AGTGCAAGCA ACAATAGCAC TATTGATTCT GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA 
TTCACTGCAG GCCGAAGAAG ATACACGGTC CAATTCACTA CAATGGTGCA GGTTAATGAG 
GAAACAGGGA ACCGACGCCC TGTGATGCTG ACTCTCCTCA GGGTACCTCG GCTGAATAAA 
AATTCAAAAA ACAGCAATGG ACAGGAACTA GAGAAGACGC TGGAAGAAAG CAAAGAAATG 
GATATCAAAC GTAAAGAAAA TAAAGGCAAT GATACCCCTT TGGCCCTAGA GAGTACAAAC 
ACTGAAAAGG AGACAAGCCT GGAGGAAACA AAAATCGGGG AGATCCTGAT CCAGGGCTTG 
ACAGAAGATA TGGTGACTGT TTTAATCCGG GCCTGCGTGA GCATGCTGGG AGTCCCTGTG 
GACCCAGATA CTTTGCATGC CACCCTTCGT CTCTGTCTGA GGCTCACCCG GGACCACAAA 
TATGCCATGA TGTTTGCAGA ACTGAAGAGT ACCCGCATGA TCTTGAATTT GACCCAGAGC 
TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC ACCCTTCTCT TAAGACACAT CATTGAGGAC 
CCCTGTACCC TTCGTCATAC CATGGAAAAG GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT 
GGTAGCACTA CCTCTGGTGT TGTGTCTGGC AGCCTCGGCT CTCGGGAGAT CAACTACATC 
CTTCGTGTCC TTGGGCCAGC CGCATGCCGC AATCCAGACA TATTCACAGA AGTGGCCAAC 
TGCTGTATCC GCATCGCCCT TCCTGCCCCT CGAGGCTCAG GAACTGCTTC AGATGATGAA 
TTTGAGAATC TTAGAATTAA AGGCCCTAAT GCTGTACAGC TGGTGAAGAC CACCCCTTTG 
AAGCCCTCAC CTCTGCCTGT CATCCCTGAT ACTATCAAGG AAGTGATCTA TGATATGCTG 
AATGCTCTGG CTGCATACCA TGCTCCAGAG GAAGCAGATA AATCTGATCC TAAACCTGGG 
GTTATGACCC AAGAGGTTGG CCAGCTCCTG CAAGACATGG GTGATGATGT ATACCAGCAG 
TACCGGTCAC TTACGCGTCA GAGCAGTGAC TTTGATACGC AGTCAGGTTT TTCCATTAAT 
AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC ACTGAGACTT CCGCATCTGG GACCTCCCAA 
GGAGAGGCTT CAACTCCAGA GGAGTCTCGA GATGGGAAGA AAGATAAAGA AGGGGACCGG 
GCCTCTGAGG AAGGCAAACA GAAAGGCAAG GGCAGCAAAC CTTTAATGCC TACCTCCACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCC 
AACTACAGCT ACACTGTGGG CCAGTCTGAA CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTTCTGG ACCACCTGCT CCCACATACC CAGAATGCAG AAGACAAGGA CACCCCTGCC 
TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT GCTGCAGGGA GTGGCACAGA TGCCCAGGTG 
GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT GGACGGGCAC TGGCTATGGC TGAGAGTACA 
GAGAAACATG CCAGGCTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAGTCCTGC 
CCCTCCACCT CCAGCTTCTA CAGCAGTGCC ACAGCGAAGA CCCAGCACAA TGGCATGAAC 
AACATCATTC GGCTTTTCCT GAAGAAGGGA CTGGTTAATG ACCTGGCCAG AGTACCTCAC 
AGCTTAGACC TGTCCAGTCC CAACATGGCC AACACAGTCA ATGCTGCTCT GAAGCCTTTG 
GAAACACTTT CCCGGATTGT GAACCAGCCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCCCAA GGAGCCTCTC AAGATTCCAG TAGCAACCAG 
CAGGACCCAG GCGAGCCTGG GGAAGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCTGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAGAATGA GCTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AGCACACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAGG GAGATGAAGA CGATGACGAC GATGGCTCTG AGATGGAATT GGATGAGGAT 
TATCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATCCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCATCC ACTGATGGTG CGCCATGCAG ACCACAGTTC TCTGACACTG 
GGCAGTGGCT CTTCAACAAC TCGTCTCACC CAGGGCATCG GGCGCAGTCA GAGGACCCTA 
AGGCAGCTGA CGGCCAATAC TGGCCACACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCCAACCCTC CTCTTATACT GCAGAGGTTG CTTGGTCCCT CAGCTGCTGC TGACATCCTT 
CAGCTGAGCA GCAGCCTTCC CCTACAAAGC CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC 
GATGACGTCC ACATCATCGC CCGTTCTGAT GATGAGCTGC TGGATGACTT TTTCCATGAT 
CAGAGCACAG CTACCAGCCA AGCAGGAACC CTGTCCAGCA TCCCCACAGC CCTGACCCGC 
TGGACAGAAG AATGCAAAGT TCTCGATGCT GAGAGCATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGTCCA TTGTCAATCA CCTGGAATTC CTGAGGGATG AGGAGCTGGA AGAAAGGCGA 
GAGAAGCGCA GGAAACAACT GGCTGAGGAA GAAACAAAGA TAACTGATAA AGGCAAAGAA 
GATAAGGAGA ACAGGGATCA GAGTGCACAG TGTACTGCAT CTAAGTCAAA TGACTCCACT 
GAACAGAATC TCTCAGATGG GACGCCTATG CCTGACAGCT ACCCAACAAC CCCATCTTCA 
ACTGATGCAG CTACATCTGA GTCCAAGGAG ACCCTTGGCA CTCTGCAATC CTCACAACAG 
CAACCAACAC TCCCAACCCC ACCAGCTTTG GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA 
GCTGGAGAAG GGGGCAGCTC TACACAGCTA TTGATGCCTG TAGAGCCAGA GGAATTGGGT 
CCCACAAGGC CAAGTGGGGA AGCAGAAACA ACTCAGATGG AGTTATCCCC AGCTCCCACT 
ATAACCTCAC TTTCCCCAGA GAGAGCTGAG GATTCTGATG CACTGACGGC TGTCAGCAGT 
CAGCTAGAAG GCTCTCCTAT GGATACAAGC AGCCTGGCTT CCTGTACCTT AGAGGAGGCT 
GTGGGTGACA CTTCAGCAGC TGGCAGTTCT GAGCAGCCCA GAGCAGGCAG CTCCACTCCT 
GGGGATGCCC CACCAGCTGT GGCGGAAGTG CAAGGCAGGA GTGATGGGTC AGGGGAATCT 
GCCCAGCCAC CTGAGGACAG CTCCCCACCT GCATCCTCTG AGAGCTCTTC CACCAGAGAT 
TCTGCCGTGG CCATTTCTGG AGCAGATTCC CGAGGAATCC TAGAAGAGCC GTTGCCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG GGTATCAGTC TCCCTGAAGG TGTGGACCCC 
TCTTTTCTGG CTGCCCTGCC TGATGACATC CGTCGGGAAG TTCTACAGAA CCAGCTAGGC 
ATTCGTCCAC CAACCCGGAC TGCCCCCTCC ACAAATAGCT CAGCGCCTGC AGTGGTGGGG 
AATCCTGGTG TGACTGAAGT GAGCCCTGAG TTTCTGGCTG CCCTGCCTCC AGCCATTCAG 
GAGGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884690.1
CDS	285..13679
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029201.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029201.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTTTGTT TCTCCTTTTG CAGTGTATTC CAATGCCTTG 
CAGGAATCAG CAAACAGTAT CTTGTATAAT GGCTTGATAG AGGAGTTGGT AGATGTCCTT 
CAGATAACGG ATAAGCAGCT TATGGAGATT AAAGCAGCTT CTTTACGAAC ATTAACATCA 
ATTGTCCACT TGGAGAGAAC TCCCAAACTC AGCAGTATTA TTGACTGTAC TGGAACTGCC 
TCCTACCATG GATTTTTGCC AGTGCTTGTA AGGAACTGTA TCCAGGCCAT GATTGATCCT 
TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG 
GCCAGCTACG ATGCTGGTGG TGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG 
AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCCGTC 
AGAGTGGTTG ACCTTATCAC CAACCTGGAT ATGGCAGCTT TTCAATCCCA TAGTGGACTT 
TCTATCTTCA TTTATAGACT TGAGCATGAA GTAGATTTGT GCCGAAAAGA ATGTCCGTTT 
GTGATCAAGC CAAAGATCCA GAGACCCAAT ACTACACAAG AAGGAGAGGA AATGGAAACT 
GATATGGATG TTGCAGATGT GGCTATGGAA AGCAGTCCAG GCTCATCCAT CTCTATGGAG 
CACAGGCTGG ATGTTGAATT AAGGGCATCA GGTTCCAGCA GCAGCACTAA CATCTCTTCT 
GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC GGCACCGGCC CTGGCCCCGG CCCCGGCCCC 
GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT 
GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT ATTCCACAAC GAGCAGCACT TCTGAAATCC 
ATGTTGAATT TCCTCAAGAA GGCCATCCAA GACCCTGCTT TCTCAGATGG CATACGACAT 
GTGATGGATG GTTCTCTGCC TACCTCCCTG AAACACATCA TCAGCAATGC AGAATACTAT 
GGCCCATCAC TCTTCCTCCT AGCTACTGAA GTGGTGACTG TGTTTGTATT TCAAGAACCA 
TCACTGCTCT CCTCACTCCA GGACAATGGA TTGACAGATG TCATGCTGCA TGCACTGCTT 
ATCAAAGATG TTCCTGCTAC CCGTGAAGTC CTTGGCTCCC TCCCAAATGT ATTCAGTGCA 
CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT TTTGTTCAGT GTCAGCCTTT TGAACGCCTC 
TTCAAAGTTC TTCTGTCTCC AGATTACCTC CCAGCCATGC GGAGGAGGAG AAGTTCTGAT 
CCCCTTGGGG ATACTGCATC CAACCTGGGG AGTGCTGTCG ATGAGCTCAT GAGACATCAG 
CCCACCCTTA AAACAGATGC AACGACTGCC ATCATCAAGT TACTTGAAGA AATCTGTAAT 
CTTGGAAGGG ACCCCAAATA CATCTGTCAG AAGCCATCAA TCCAGAAGGC AGATGGCACT 
GCCACTGCTC CTCCCCCAAG GTCTAATCAT GCCGCAGAAG AAGCCTCTAG TGAGGATGAG 
GAGGAAGAGG AAGTACAGGC CATGCAGAGC TTTAATTCTA CCCAGCAAAA TGAAACTGAG 
CCTAATCAGC AGGTTGTTGG TACAGAGGAA CGTATTCCTA TTCCCCTCAT GGATTACATC 
CTTAATGTGA TGAAATTTGT GGAATCTATT CTGAGCAACA ATACAACAGA TGACCACTGC 
CAGGAATTTG TGAATCAGAA AGGACTGTTG CCTTTGGTTA CCATTTTGGG TCTTCCCAAT 
CTGCCCATTG ACTTTCCCAC ATCTGCTGCC TGTCAGGCTG TTGCAGGTGT CTGCAAATCC 
ATATTGACAC TGTCACATGA ACCCAAAGTC CTTCAAGAGG GTCTCCTTCA GTTGGACTCC 
ATCCTCTCCT CCCTGGAGCC CTTACACCGC CCCATTGAAT CCCCTGGGGG CTCAGTGTTG 
TTGCGAGAAC TGGCTTGCGC AGGCAATGTT GCTGATGCTA CCCTCTCAGC CCAGGCCACA 
CCTCTGCTGC ATGCACTCAC TGCTGCCCAT GCCTACATCA TGATGTTTGT TCATACTTGC 
AGAGTTGGAC AGAGTGAAAT TCGTTCCATC TCCGTAAACC AGTGGGGCTC TCAATTGGGT 
CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA TACTGTTCCC TGGTGTGGGA AAGCACTGTC 
CTCCTCTCTC TGTGTACCCC AAACAGCCTA CCATCTGGGT GTGAATTTGG CCAGGCAGAT 
ATGCAGAAAC TGGTTCCAAA GGATGAGAAG GCAGGTACGA CCCAGGGCGG AAAAAGATCA 
GATGGGGAAC AGGATGGAGC AGCTGGAAGT ATGGATGCTT CTACCCAGGG CTTATTAGAA 
GGCATTGGGC TAGATGGTGA CACATTGGCT CCCATGGAGA CAGATGAACC TACTGCTTCA 
GACTCTAAGG GCAAATCTAA AATCACACCA GCAATGGCTG CCAGAATTAA GCAAATCAAG 
CCTTTGTTAT CAGCTTCCTC CAGATTAGGC CGAGCACTTG CTGAGCTATT TGGACTTCTT 
GTTAAACTTT GTGTGGGATC TCCTGTCCGC CAGAGAAGGA GCCATCATGC TGCCAGCACC 
ACTACAGCAC CGACACCTGC CGCGCGATCA ACAGCCTCAG CTCTCACTAA GCTCTTGACT 
AAGGGGTTAT CTTGGCAGCC CCCACCATAT ACACCTACTC CCCGATTCAG GCTGACATTC 
TTCATCTGTT CAGTTGGTTT CACATCCCCA ATGCTGTTTG ATGAGAGGAA GTATCCCTAC 
CACCTCATGC TGCAAAAATT TCTCTGCTCC GGAGGCCACA ATGCTCTTTT TGAAACTTTC 
AACTGGGCTC TGTCCATGGG AGGTAAAGTT CCTGTTTCTG AGGGATTGGA ACACTCAGAC 
TTGCCTGATG GCACAGGAGA ATTCCTAGAT GCCTGGCTTA TGCTGGTGGA GAAGATGGTG 
AATCCCACCA CGGTGCTTGA ATCTCCACAT TCGCTGCCTG CCAAATTGCC TGGAGGTGTC 
CAGAACTTTC CCCAGTTCAG TGCACTGCGC TTCCTTGTGG TAACTCAGAA AGCAGCCTTT 
ACTTGCATCA AAAACTTATG GAACCGGAAA CCCCTGAAGG TATATGGTGG ACGAATGGCT 
GAATCGATGC TGGCCATTCT ATGCCACATC CTCCGAGGAG AACCTGTGAT TCGAGAGAGA 
CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA GAAGAGGATA CAGGGCAAGA GGAAGGTGGC 
TCCCGCCGGG AACCTCAAGT CAACCAGCAA CAACTGCAAC AGCTCATGGA CATGGGCTTC 
ACAAGGGAAC ATGCAATGGA GGCACTGTTG AACACCAGCA CCATGGAGCA GGCCACAGAG 
TACCTTTTAA CCCACCCTCC TCCAATCATG GGAGGAGTTG TTCGGGATCT CAGCATGTCT 
GAAGAGGACC AGATGATGAG AGCAATTGCT ATGTCTCTGG GACAGGATAT TCCAATGGAT 
CAAAGGGCAG AGTCACCTGA GGAAGTTGCT TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT 
CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA TGTCTAGAGA AGTTCCAGGA TGCTGACCCG 
TTGGAACAAG ATGAGCTCCA CACTTTCACA GATACTATGT TGCCAGGCTG CTTCCACCTT 
CTTGATGAGC TGCCAGACAC AGTATACCGT GTGTGTGACC TGATCATGAC AGCAATCAAA 
CGTAATGGAG CAGATTATCG TGACATGATT CTGAAGCAAG TAGTCAATCA GGTGTGGGAA 
GCTGCTGATG TATTGATCAA AGCTGCTCTT CCCCTGACAA CAAGTGACAC AAAAACCGTG 
TCAGAGTGGA TAAGTCAGAT GGCCACACTG CCCCAGGCCT CCAATTTGGC TACTAGAATC 
TTGCTTTTAA CGCTACTTTT TGAGGAGTTG AAGCTACCTT GTGCTTGGGT GGTTGAATCA 
AGTGGCATCC TTAATGTCCT AATCAAACTC TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA 
GCCAAGGAGC AGAAGGAAGT CCAGACCCCA AAGTGGATCA CACCAGTGTT GCTCCTGATT 
GATTTCTATG AAAAGACAGC CATCTCCTCA AAAAGGAGAG CCCAGATGAC TAAGTACCTG 
CAATCCAACA GCAACAACTG GCGCTGGTTT GATGATCGCT CTGGGCGTTG GTGTAGTTAC 
AGTGCAAGCA ACAATAGCAC TATTGATTCT GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA 
TTCACTGCAG GCCGAAGAAG ATACACGGTC CAATTCACTA CAATGGTGCA GGTTAATGAG 
GAAACAGGGA ACCGACGCCC TGTGATGCTG ACTCTCCTCA GGGTACCTCG GCTGAATAAA 
AATTCAAAAA ACAGCAATGG ACAGGAACTA GAGAAGACGC TGGAAGAAAG CAAAGAAATG 
GATATCAAAC GTAAAGAAAA TAAAGGCAAT GATACCCCTT TGGCCCTAGA GAGTACAAAC 
ACTGAAAAGG AGACAAGCCT GGAGGAAACA AAAATCGGGG AGATCCTGAT CCAGGGCTTG 
ACAGAAGATA TGGTGACTGT TTTAATCCGG GCCTGCGTGA GCATGCTGGG AGTCCCTGTG 
GACCCAGATA CTTTGCATGC CACCCTTCGT CTCTGTCTGA GGCTCACCCG GGACCACAAA 
TATGCCATGA TGTTTGCAGA ACTGAAGAGT ACCCGCATGA TCTTGAATTT GACCCAGAGC 
TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC ACCCTTCTCT TAAGACACAT CATTGAGGAC 
CCCTGTACCC TTCGTCATAC CATGGAAAAG GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT 
GGTAGCACTA CCTCTGGTGT TGTGTCTGGC AGCCTCGGCT CTCGGGAGAT CAACTACATC 
CTTCGTGTCC TTGGGCCAGC CGCATGCCGC AATCCAGACA TATTCACAGA AGTGGCCAAC 
TGCTGTATCC GCATCGCCCT TCCTGCCCCT CGAGGCTCAG GAACTGCTTC AGATGATGAA 
TTTGAGAATC TTAGAATTAA AGGCCCTAAT GCTGTACAGC TGGTGAAGAC CACCCCTTTG 
AAGCCCTCAC CTCTGCCTGT CATCCCTGAT ACTATCAAGG AAGTGATCTA TGATATGCTG 
AATGCTCTGG CTGCATACCA TGCTCCAGAG GAAGCAGATA AATCTGATCC TAAACCTGGG 
GTTATGACCC AAGAGGTTGG CCAGCTCCTG CAAGACATGG GTGATGATGT ATACCAGCAG 
TACCGGTCAC TTACGCGTCA GAGCAGTGAC TTTGATACGC AGTCAGGTTT TTCCATTAAT 
AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC ACTGAGACTT CCGCATCTGG GACCTCCCAA 
GGAGAGGCTT CAACTCCAGA GGAGTCTCGA GATGGGAAGA AAGATAAAGA AGGGGACCGG 
GCCTCTGAGG AAGGCAAACA GAAAGGCAAG GGCAGCAAAC CTTTAATGCC TACCTCCACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCC 
AACTACAGCT ACACTGTGGG CCAGTCTGAA CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTTCTGG ACCACCTGCT CCCACATACC CAGAATGCAG AAGACAAGGA CACCCCTGCC 
TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT GCTGCAGGGA GTGGCACAGA TGCCCAGGTG 
GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT GGACGGGCAC TGGCTATGGC TGAGAGTACA 
GAGAAACATG CCAGGCTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAGTCCTGC 
CCCTCCACCT CCAGCTTCTA CAGCAGTGCC ACAGCGAAGA CCCAGCACAA TGGCATGAAC 
AACATCATTC GGCTTTTCCT GAAGAAGGGA CTGGTTAATG ACCTGGCCAG AGTACCTCAC 
AGCTTAGACC TGTCCAGTCC CAACATGGCC AACACAGTCA ATGCTGCTCT GAAGCCTTTG 
GAAACACTTT CCCGGATTGT GAACCAGCCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCCCAA GGAGCCTCTC AAGATTCCAG TAGCAACCAG 
CAGGACCCAG GCGAGCCTGG GGAAGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCTGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAGAATGA GCTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AGCACACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAGG GAGATGAAGA CGATGACGAC GATGGCTCTG AGATGGAATT GGATGAGGAT 
TATCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATCCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCATCC ACTGATGGTG CGCCATGCAG ACCACAGTTC TCTGACACTG 
GGCAGTGGCT CTTCAACAAC TCGTCTCACC CAGGGCATCG GGCGCAGTCA GAGGACCCTA 
AGGCAGCTGA CGGCCAATAC TGGCCACACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCCAACCCTC CTCTTATACT GCAGAGGTTG CTTGGTCCCT CAGCTGCTGC TGACATCCTT 
CAGCTGAGCA GCAGCCTTCC CCTACAAAGC CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC 
GATGACGTCC ACATCATCGC CCGTTCTGAT GATGAGCTGC TGGATGACTT TTTCCATGAT 
CAGAGCACAG CTACCAGCCA AGCAGGAACC CTGTCCAGCA TCCCCACAGC CCTGACCCGC 
TGGACAGAAG AATGCAAAGT TCTCGATGCT GAGAGCATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGTCCA TTGTCAATCA CCTGGAATTC CTGAGGGATG AGGAGCTGGA AGAAAGGCGA 
GAGAAGCGCA GGAAACAACT GGCTGAGGAA GAAACAAAGA TAACTGATAA AGGCAAAGAA 
GATAAGGAGA ACAGGGATCA GAGTGCACAG TGTACTGCAT CTAAGTCAAA TGACTCCACT 
GAACAGAATC TCTCAGATGG GACGCCTATG CCTGACAGCT ACCCAACAAC CCCATCTTCA 
ACTGATGCAG CTACATCTGA GTCCAAGGAG ACCCTTGGCA CTCTGCAATC CTCACAACAG 
CAACCAACAC TCCCAACCCC ACCAGCTTTG GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA 
GCTGGAGAAG GGGGCAGCTC TACACAGCTA TTGATGCCTG TAGAGCCAGA GGAATTGGGT 
CCCACAAGGC CAAGTGGGGA AGCAGAAACA ACTCAGATGG AGTTATCCCC AGCTCCCACT 
ATAACCTCAC TTTCCCCAGA GAGAGCTGAG GATTCTGATG CACTGACGGC TGTCAGCAGT 
CAGCTAGAAG GCTCTCCTAT GGATACAAGC AGCCTGGCTT CCTGTACCTT AGAGGAGGCT 
GTGGGTGACA CTTCAGCAGC TGGCAGTTCT GAGCAGCCCA GAGCAGGCAG CTCCACTCCT 
GGGGATGCCC CACCAGCTGT GGCGGAAGTG CAAGGCAGGA GTGATGGGTC AGGGGAATCT 
GCCCAGCCAC CTGAGGACAG CTCCCCACCT GCATCCTCTG AGAGCTCTTC CACCAGAGAT 
TCTGCCGTGG CCATTTCTGG AGCAGATTCC CGAGGAATCC TAGAAGAGCC GTTGCCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG GGTATCAGTC TCCCTGAAGG TGTGGACCCC 
TCTTTTCTGG CTGCCCTGCC TGATGACATC CGTCGGGAAG TTCTACAGAA CCAGCTAGGC 
ATTCGTCCAC CAACCCGGAC TGCCCCCTCC ACAAATAGCT CAGCGCCTGC AGTGGTGGGG 
AATCCTGGTG TGACTGAAGT GAGCCCTGAG TTTCTGGCTG CCCTGCCTCC AGCCATTCAG 
GAGGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029203.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884692.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X13, mRNA.

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CloneID	OHu99119
Clone ID Related Accession (Same CDS sequence)	XM_017029199.1 , XM_017029201.1 , XM_017029203.1 , XM_017029200.1 , XM_017029202.1 , XM_017029198.1 , XM_017029198.2
Accession Version	XM_017029203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTTTGTT TCTCCTTTTG CAGTGTATTC CAATGCCTTG 
CAGGAATCAG CAAACAGTAT CTTGTATAAT GGCTTGATAG AGGAGTTGGT AGATGTCCTT 
CAGATAACGG ATAAGCAGCT TATGGAGATT AAAGCAGCTT CTTTACGAAC ATTAACATCA 
ATTGTCCACT TGGAGAGAAC TCCCAAACTC AGCAGTATTA TTGACTGTAC TGGAACTGCC 
TCCTACCATG GATTTTTGCC AGTGCTTGTA AGGAACTGTA TCCAGGCCAT GATTGATCCT 
TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG 
GCCAGCTACG ATGCTGGTGG TGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG 
AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCCGTC 
AGAGTGGTTG ACCTTATCAC CAACCTGGAT ATGGCAGCTT TTCAATCCCA TAGTGGACTT 
TCTATCTTCA TTTATAGACT TGAGCATGAA GTAGATTTGT GCCGAAAAGA ATGTCCGTTT 
GTGATCAAGC CAAAGATCCA GAGACCCAAT ACTACACAAG AAGGAGAGGA AATGGAAACT 
GATATGGATG TTGCAGATGT GGCTATGGAA AGCAGTCCAG GCTCATCCAT CTCTATGGAG 
CACAGGCTGG ATGTTGAATT AAGGGCATCA GGTTCCAGCA GCAGCACTAA CATCTCTTCT 
GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC GGCACCGGCC CTGGCCCCGG CCCCGGCCCC 
GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT 
GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT ATTCCACAAC GAGCAGCACT TCTGAAATCC 
ATGTTGAATT TCCTCAAGAA GGCCATCCAA GACCCTGCTT TCTCAGATGG CATACGACAT 
GTGATGGATG GTTCTCTGCC TACCTCCCTG AAACACATCA TCAGCAATGC AGAATACTAT 
GGCCCATCAC TCTTCCTCCT AGCTACTGAA GTGGTGACTG TGTTTGTATT TCAAGAACCA 
TCACTGCTCT CCTCACTCCA GGACAATGGA TTGACAGATG TCATGCTGCA TGCACTGCTT 
ATCAAAGATG TTCCTGCTAC CCGTGAAGTC CTTGGCTCCC TCCCAAATGT ATTCAGTGCA 
CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT TTTGTTCAGT GTCAGCCTTT TGAACGCCTC 
TTCAAAGTTC TTCTGTCTCC AGATTACCTC CCAGCCATGC GGAGGAGGAG AAGTTCTGAT 
CCCCTTGGGG ATACTGCATC CAACCTGGGG AGTGCTGTCG ATGAGCTCAT GAGACATCAG 
CCCACCCTTA AAACAGATGC AACGACTGCC ATCATCAAGT TACTTGAAGA AATCTGTAAT 
CTTGGAAGGG ACCCCAAATA CATCTGTCAG AAGCCATCAA TCCAGAAGGC AGATGGCACT 
GCCACTGCTC CTCCCCCAAG GTCTAATCAT GCCGCAGAAG AAGCCTCTAG TGAGGATGAG 
GAGGAAGAGG AAGTACAGGC CATGCAGAGC TTTAATTCTA CCCAGCAAAA TGAAACTGAG 
CCTAATCAGC AGGTTGTTGG TACAGAGGAA CGTATTCCTA TTCCCCTCAT GGATTACATC 
CTTAATGTGA TGAAATTTGT GGAATCTATT CTGAGCAACA ATACAACAGA TGACCACTGC 
CAGGAATTTG TGAATCAGAA AGGACTGTTG CCTTTGGTTA CCATTTTGGG TCTTCCCAAT 
CTGCCCATTG ACTTTCCCAC ATCTGCTGCC TGTCAGGCTG TTGCAGGTGT CTGCAAATCC 
ATATTGACAC TGTCACATGA ACCCAAAGTC CTTCAAGAGG GTCTCCTTCA GTTGGACTCC 
ATCCTCTCCT CCCTGGAGCC CTTACACCGC CCCATTGAAT CCCCTGGGGG CTCAGTGTTG 
TTGCGAGAAC TGGCTTGCGC AGGCAATGTT GCTGATGCTA CCCTCTCAGC CCAGGCCACA 
CCTCTGCTGC ATGCACTCAC TGCTGCCCAT GCCTACATCA TGATGTTTGT TCATACTTGC 
AGAGTTGGAC AGAGTGAAAT TCGTTCCATC TCCGTAAACC AGTGGGGCTC TCAATTGGGT 
CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA TACTGTTCCC TGGTGTGGGA AAGCACTGTC 
CTCCTCTCTC TGTGTACCCC AAACAGCCTA CCATCTGGGT GTGAATTTGG CCAGGCAGAT 
ATGCAGAAAC TGGTTCCAAA GGATGAGAAG GCAGGTACGA CCCAGGGCGG AAAAAGATCA 
GATGGGGAAC AGGATGGAGC AGCTGGAAGT ATGGATGCTT CTACCCAGGG CTTATTAGAA 
GGCATTGGGC TAGATGGTGA CACATTGGCT CCCATGGAGA CAGATGAACC TACTGCTTCA 
GACTCTAAGG GCAAATCTAA AATCACACCA GCAATGGCTG CCAGAATTAA GCAAATCAAG 
CCTTTGTTAT CAGCTTCCTC CAGATTAGGC CGAGCACTTG CTGAGCTATT TGGACTTCTT 
GTTAAACTTT GTGTGGGATC TCCTGTCCGC CAGAGAAGGA GCCATCATGC TGCCAGCACC 
ACTACAGCAC CGACACCTGC CGCGCGATCA ACAGCCTCAG CTCTCACTAA GCTCTTGACT 
AAGGGGTTAT CTTGGCAGCC CCCACCATAT ACACCTACTC CCCGATTCAG GCTGACATTC 
TTCATCTGTT CAGTTGGTTT CACATCCCCA ATGCTGTTTG ATGAGAGGAA GTATCCCTAC 
CACCTCATGC TGCAAAAATT TCTCTGCTCC GGAGGCCACA ATGCTCTTTT TGAAACTTTC 
AACTGGGCTC TGTCCATGGG AGGTAAAGTT CCTGTTTCTG AGGGATTGGA ACACTCAGAC 
TTGCCTGATG GCACAGGAGA ATTCCTAGAT GCCTGGCTTA TGCTGGTGGA GAAGATGGTG 
AATCCCACCA CGGTGCTTGA ATCTCCACAT TCGCTGCCTG CCAAATTGCC TGGAGGTGTC 
CAGAACTTTC CCCAGTTCAG TGCACTGCGC TTCCTTGTGG TAACTCAGAA AGCAGCCTTT 
ACTTGCATCA AAAACTTATG GAACCGGAAA CCCCTGAAGG TATATGGTGG ACGAATGGCT 
GAATCGATGC TGGCCATTCT ATGCCACATC CTCCGAGGAG AACCTGTGAT TCGAGAGAGA 
CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA GAAGAGGATA CAGGGCAAGA GGAAGGTGGC 
TCCCGCCGGG AACCTCAAGT CAACCAGCAA CAACTGCAAC AGCTCATGGA CATGGGCTTC 
ACAAGGGAAC ATGCAATGGA GGCACTGTTG AACACCAGCA CCATGGAGCA GGCCACAGAG 
TACCTTTTAA CCCACCCTCC TCCAATCATG GGAGGAGTTG TTCGGGATCT CAGCATGTCT 
GAAGAGGACC AGATGATGAG AGCAATTGCT ATGTCTCTGG GACAGGATAT TCCAATGGAT 
CAAAGGGCAG AGTCACCTGA GGAAGTTGCT TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT 
CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA TGTCTAGAGA AGTTCCAGGA TGCTGACCCG 
TTGGAACAAG ATGAGCTCCA CACTTTCACA GATACTATGT TGCCAGGCTG CTTCCACCTT 
CTTGATGAGC TGCCAGACAC AGTATACCGT GTGTGTGACC TGATCATGAC AGCAATCAAA 
CGTAATGGAG CAGATTATCG TGACATGATT CTGAAGCAAG TAGTCAATCA GGTGTGGGAA 
GCTGCTGATG TATTGATCAA AGCTGCTCTT CCCCTGACAA CAAGTGACAC AAAAACCGTG 
TCAGAGTGGA TAAGTCAGAT GGCCACACTG CCCCAGGCCT CCAATTTGGC TACTAGAATC 
TTGCTTTTAA CGCTACTTTT TGAGGAGTTG AAGCTACCTT GTGCTTGGGT GGTTGAATCA 
AGTGGCATCC TTAATGTCCT AATCAAACTC TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA 
GCCAAGGAGC AGAAGGAAGT CCAGACCCCA AAGTGGATCA CACCAGTGTT GCTCCTGATT 
GATTTCTATG AAAAGACAGC CATCTCCTCA AAAAGGAGAG CCCAGATGAC TAAGTACCTG 
CAATCCAACA GCAACAACTG GCGCTGGTTT GATGATCGCT CTGGGCGTTG GTGTAGTTAC 
AGTGCAAGCA ACAATAGCAC TATTGATTCT GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA 
TTCACTGCAG GCCGAAGAAG ATACACGGTC CAATTCACTA CAATGGTGCA GGTTAATGAG 
GAAACAGGGA ACCGACGCCC TGTGATGCTG ACTCTCCTCA GGGTACCTCG GCTGAATAAA 
AATTCAAAAA ACAGCAATGG ACAGGAACTA GAGAAGACGC TGGAAGAAAG CAAAGAAATG 
GATATCAAAC GTAAAGAAAA TAAAGGCAAT GATACCCCTT TGGCCCTAGA GAGTACAAAC 
ACTGAAAAGG AGACAAGCCT GGAGGAAACA AAAATCGGGG AGATCCTGAT CCAGGGCTTG 
ACAGAAGATA TGGTGACTGT TTTAATCCGG GCCTGCGTGA GCATGCTGGG AGTCCCTGTG 
GACCCAGATA CTTTGCATGC CACCCTTCGT CTCTGTCTGA GGCTCACCCG GGACCACAAA 
TATGCCATGA TGTTTGCAGA ACTGAAGAGT ACCCGCATGA TCTTGAATTT GACCCAGAGC 
TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC ACCCTTCTCT TAAGACACAT CATTGAGGAC 
CCCTGTACCC TTCGTCATAC CATGGAAAAG GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT 
GGTAGCACTA CCTCTGGTGT TGTGTCTGGC AGCCTCGGCT CTCGGGAGAT CAACTACATC 
CTTCGTGTCC TTGGGCCAGC CGCATGCCGC AATCCAGACA TATTCACAGA AGTGGCCAAC 
TGCTGTATCC GCATCGCCCT TCCTGCCCCT CGAGGCTCAG GAACTGCTTC AGATGATGAA 
TTTGAGAATC TTAGAATTAA AGGCCCTAAT GCTGTACAGC TGGTGAAGAC CACCCCTTTG 
AAGCCCTCAC CTCTGCCTGT CATCCCTGAT ACTATCAAGG AAGTGATCTA TGATATGCTG 
AATGCTCTGG CTGCATACCA TGCTCCAGAG GAAGCAGATA AATCTGATCC TAAACCTGGG 
GTTATGACCC AAGAGGTTGG CCAGCTCCTG CAAGACATGG GTGATGATGT ATACCAGCAG 
TACCGGTCAC TTACGCGTCA GAGCAGTGAC TTTGATACGC AGTCAGGTTT TTCCATTAAT 
AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC ACTGAGACTT CCGCATCTGG GACCTCCCAA 
GGAGAGGCTT CAACTCCAGA GGAGTCTCGA GATGGGAAGA AAGATAAAGA AGGGGACCGG 
GCCTCTGAGG AAGGCAAACA GAAAGGCAAG GGCAGCAAAC CTTTAATGCC TACCTCCACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCC 
AACTACAGCT ACACTGTGGG CCAGTCTGAA CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTTCTGG ACCACCTGCT CCCACATACC CAGAATGCAG AAGACAAGGA CACCCCTGCC 
TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT GCTGCAGGGA GTGGCACAGA TGCCCAGGTG 
GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT GGACGGGCAC TGGCTATGGC TGAGAGTACA 
GAGAAACATG CCAGGCTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAGTCCTGC 
CCCTCCACCT CCAGCTTCTA CAGCAGTGCC ACAGCGAAGA CCCAGCACAA TGGCATGAAC 
AACATCATTC GGCTTTTCCT GAAGAAGGGA CTGGTTAATG ACCTGGCCAG AGTACCTCAC 
AGCTTAGACC TGTCCAGTCC CAACATGGCC AACACAGTCA ATGCTGCTCT GAAGCCTTTG 
GAAACACTTT CCCGGATTGT GAACCAGCCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCCCAA GGAGCCTCTC AAGATTCCAG TAGCAACCAG 
CAGGACCCAG GCGAGCCTGG GGAAGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCTGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAGAATGA GCTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AGCACACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAGG GAGATGAAGA CGATGACGAC GATGGCTCTG AGATGGAATT GGATGAGGAT 
TATCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATCCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCATCC ACTGATGGTG CGCCATGCAG ACCACAGTTC TCTGACACTG 
GGCAGTGGCT CTTCAACAAC TCGTCTCACC CAGGGCATCG GGCGCAGTCA GAGGACCCTA 
AGGCAGCTGA CGGCCAATAC TGGCCACACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCCAACCCTC CTCTTATACT GCAGAGGTTG CTTGGTCCCT CAGCTGCTGC TGACATCCTT 
CAGCTGAGCA GCAGCCTTCC CCTACAAAGC CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC 
GATGACGTCC ACATCATCGC CCGTTCTGAT GATGAGCTGC TGGATGACTT TTTCCATGAT 
CAGAGCACAG CTACCAGCCA AGCAGGAACC CTGTCCAGCA TCCCCACAGC CCTGACCCGC 
TGGACAGAAG AATGCAAAGT TCTCGATGCT GAGAGCATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGTCCA TTGTCAATCA CCTGGAATTC CTGAGGGATG AGGAGCTGGA AGAAAGGCGA 
GAGAAGCGCA GGAAACAACT GGCTGAGGAA GAAACAAAGA TAACTGATAA AGGCAAAGAA 
GATAAGGAGA ACAGGGATCA GAGTGCACAG TGTACTGCAT CTAAGTCAAA TGACTCCACT 
GAACAGAATC TCTCAGATGG GACGCCTATG CCTGACAGCT ACCCAACAAC CCCATCTTCA 
ACTGATGCAG CTACATCTGA GTCCAAGGAG ACCCTTGGCA CTCTGCAATC CTCACAACAG 
CAACCAACAC TCCCAACCCC ACCAGCTTTG GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA 
GCTGGAGAAG GGGGCAGCTC TACACAGCTA TTGATGCCTG TAGAGCCAGA GGAATTGGGT 
CCCACAAGGC CAAGTGGGGA AGCAGAAACA ACTCAGATGG AGTTATCCCC AGCTCCCACT 
ATAACCTCAC TTTCCCCAGA GAGAGCTGAG GATTCTGATG CACTGACGGC TGTCAGCAGT 
CAGCTAGAAG GCTCTCCTAT GGATACAAGC AGCCTGGCTT CCTGTACCTT AGAGGAGGCT 
GTGGGTGACA CTTCAGCAGC TGGCAGTTCT GAGCAGCCCA GAGCAGGCAG CTCCACTCCT 
GGGGATGCCC CACCAGCTGT GGCGGAAGTG CAAGGCAGGA GTGATGGGTC AGGGGAATCT 
GCCCAGCCAC CTGAGGACAG CTCCCCACCT GCATCCTCTG AGAGCTCTTC CACCAGAGAT 
TCTGCCGTGG CCATTTCTGG AGCAGATTCC CGAGGAATCC TAGAAGAGCC GTTGCCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG GGTATCAGTC TCCCTGAAGG TGTGGACCCC 
TCTTTTCTGG CTGCCCTGCC TGATGACATC CGTCGGGAAG TTCTACAGAA CCAGCTAGGC 
ATTCGTCCAC CAACCCGGAC TGCCCCCTCC ACAAATAGCT CAGCGCCTGC AGTGGTGGGG 
AATCCTGGTG TGACTGAAGT GAGCCCTGAG TTTCTGGCTG CCCTGCCTCC AGCCATTCAG 
GAGGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884692.1
CDS	267..13661
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029203.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029203.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTTTGTT TCTCCTTTTG CAGTGTATTC CAATGCCTTG 
CAGGAATCAG CAAACAGTAT CTTGTATAAT GGCTTGATAG AGGAGTTGGT AGATGTCCTT 
CAGATAACGG ATAAGCAGCT TATGGAGATT AAAGCAGCTT CTTTACGAAC ATTAACATCA 
ATTGTCCACT TGGAGAGAAC TCCCAAACTC AGCAGTATTA TTGACTGTAC TGGAACTGCC 
TCCTACCATG GATTTTTGCC AGTGCTTGTA AGGAACTGTA TCCAGGCCAT GATTGATCCT 
TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG 
GCCAGCTACG ATGCTGGTGG TGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG 
AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCCGTC 
AGAGTGGTTG ACCTTATCAC CAACCTGGAT ATGGCAGCTT TTCAATCCCA TAGTGGACTT 
TCTATCTTCA TTTATAGACT TGAGCATGAA GTAGATTTGT GCCGAAAAGA ATGTCCGTTT 
GTGATCAAGC CAAAGATCCA GAGACCCAAT ACTACACAAG AAGGAGAGGA AATGGAAACT 
GATATGGATG TTGCAGATGT GGCTATGGAA AGCAGTCCAG GCTCATCCAT CTCTATGGAG 
CACAGGCTGG ATGTTGAATT AAGGGCATCA GGTTCCAGCA GCAGCACTAA CATCTCTTCT 
GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC GGCACCGGCC CTGGCCCCGG CCCCGGCCCC 
GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT 
GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT ATTCCACAAC GAGCAGCACT TCTGAAATCC 
ATGTTGAATT TCCTCAAGAA GGCCATCCAA GACCCTGCTT TCTCAGATGG CATACGACAT 
GTGATGGATG GTTCTCTGCC TACCTCCCTG AAACACATCA TCAGCAATGC AGAATACTAT 
GGCCCATCAC TCTTCCTCCT AGCTACTGAA GTGGTGACTG TGTTTGTATT TCAAGAACCA 
TCACTGCTCT CCTCACTCCA GGACAATGGA TTGACAGATG TCATGCTGCA TGCACTGCTT 
ATCAAAGATG TTCCTGCTAC CCGTGAAGTC CTTGGCTCCC TCCCAAATGT ATTCAGTGCA 
CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT TTTGTTCAGT GTCAGCCTTT TGAACGCCTC 
TTCAAAGTTC TTCTGTCTCC AGATTACCTC CCAGCCATGC GGAGGAGGAG AAGTTCTGAT 
CCCCTTGGGG ATACTGCATC CAACCTGGGG AGTGCTGTCG ATGAGCTCAT GAGACATCAG 
CCCACCCTTA AAACAGATGC AACGACTGCC ATCATCAAGT TACTTGAAGA AATCTGTAAT 
CTTGGAAGGG ACCCCAAATA CATCTGTCAG AAGCCATCAA TCCAGAAGGC AGATGGCACT 
GCCACTGCTC CTCCCCCAAG GTCTAATCAT GCCGCAGAAG AAGCCTCTAG TGAGGATGAG 
GAGGAAGAGG AAGTACAGGC CATGCAGAGC TTTAATTCTA CCCAGCAAAA TGAAACTGAG 
CCTAATCAGC AGGTTGTTGG TACAGAGGAA CGTATTCCTA TTCCCCTCAT GGATTACATC 
CTTAATGTGA TGAAATTTGT GGAATCTATT CTGAGCAACA ATACAACAGA TGACCACTGC 
CAGGAATTTG TGAATCAGAA AGGACTGTTG CCTTTGGTTA CCATTTTGGG TCTTCCCAAT 
CTGCCCATTG ACTTTCCCAC ATCTGCTGCC TGTCAGGCTG TTGCAGGTGT CTGCAAATCC 
ATATTGACAC TGTCACATGA ACCCAAAGTC CTTCAAGAGG GTCTCCTTCA GTTGGACTCC 
ATCCTCTCCT CCCTGGAGCC CTTACACCGC CCCATTGAAT CCCCTGGGGG CTCAGTGTTG 
TTGCGAGAAC TGGCTTGCGC AGGCAATGTT GCTGATGCTA CCCTCTCAGC CCAGGCCACA 
CCTCTGCTGC ATGCACTCAC TGCTGCCCAT GCCTACATCA TGATGTTTGT TCATACTTGC 
AGAGTTGGAC AGAGTGAAAT TCGTTCCATC TCCGTAAACC AGTGGGGCTC TCAATTGGGT 
CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA TACTGTTCCC TGGTGTGGGA AAGCACTGTC 
CTCCTCTCTC TGTGTACCCC AAACAGCCTA CCATCTGGGT GTGAATTTGG CCAGGCAGAT 
ATGCAGAAAC TGGTTCCAAA GGATGAGAAG GCAGGTACGA CCCAGGGCGG AAAAAGATCA 
GATGGGGAAC AGGATGGAGC AGCTGGAAGT ATGGATGCTT CTACCCAGGG CTTATTAGAA 
GGCATTGGGC TAGATGGTGA CACATTGGCT CCCATGGAGA CAGATGAACC TACTGCTTCA 
GACTCTAAGG GCAAATCTAA AATCACACCA GCAATGGCTG CCAGAATTAA GCAAATCAAG 
CCTTTGTTAT CAGCTTCCTC CAGATTAGGC CGAGCACTTG CTGAGCTATT TGGACTTCTT 
GTTAAACTTT GTGTGGGATC TCCTGTCCGC CAGAGAAGGA GCCATCATGC TGCCAGCACC 
ACTACAGCAC CGACACCTGC CGCGCGATCA ACAGCCTCAG CTCTCACTAA GCTCTTGACT 
AAGGGGTTAT CTTGGCAGCC CCCACCATAT ACACCTACTC CCCGATTCAG GCTGACATTC 
TTCATCTGTT CAGTTGGTTT CACATCCCCA ATGCTGTTTG ATGAGAGGAA GTATCCCTAC 
CACCTCATGC TGCAAAAATT TCTCTGCTCC GGAGGCCACA ATGCTCTTTT TGAAACTTTC 
AACTGGGCTC TGTCCATGGG AGGTAAAGTT CCTGTTTCTG AGGGATTGGA ACACTCAGAC 
TTGCCTGATG GCACAGGAGA ATTCCTAGAT GCCTGGCTTA TGCTGGTGGA GAAGATGGTG 
AATCCCACCA CGGTGCTTGA ATCTCCACAT TCGCTGCCTG CCAAATTGCC TGGAGGTGTC 
CAGAACTTTC CCCAGTTCAG TGCACTGCGC TTCCTTGTGG TAACTCAGAA AGCAGCCTTT 
ACTTGCATCA AAAACTTATG GAACCGGAAA CCCCTGAAGG TATATGGTGG ACGAATGGCT 
GAATCGATGC TGGCCATTCT ATGCCACATC CTCCGAGGAG AACCTGTGAT TCGAGAGAGA 
CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA GAAGAGGATA CAGGGCAAGA GGAAGGTGGC 
TCCCGCCGGG AACCTCAAGT CAACCAGCAA CAACTGCAAC AGCTCATGGA CATGGGCTTC 
ACAAGGGAAC ATGCAATGGA GGCACTGTTG AACACCAGCA CCATGGAGCA GGCCACAGAG 
TACCTTTTAA CCCACCCTCC TCCAATCATG GGAGGAGTTG TTCGGGATCT CAGCATGTCT 
GAAGAGGACC AGATGATGAG AGCAATTGCT ATGTCTCTGG GACAGGATAT TCCAATGGAT 
CAAAGGGCAG AGTCACCTGA GGAAGTTGCT TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT 
CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA TGTCTAGAGA AGTTCCAGGA TGCTGACCCG 
TTGGAACAAG ATGAGCTCCA CACTTTCACA GATACTATGT TGCCAGGCTG CTTCCACCTT 
CTTGATGAGC TGCCAGACAC AGTATACCGT GTGTGTGACC TGATCATGAC AGCAATCAAA 
CGTAATGGAG CAGATTATCG TGACATGATT CTGAAGCAAG TAGTCAATCA GGTGTGGGAA 
GCTGCTGATG TATTGATCAA AGCTGCTCTT CCCCTGACAA CAAGTGACAC AAAAACCGTG 
TCAGAGTGGA TAAGTCAGAT GGCCACACTG CCCCAGGCCT CCAATTTGGC TACTAGAATC 
TTGCTTTTAA CGCTACTTTT TGAGGAGTTG AAGCTACCTT GTGCTTGGGT GGTTGAATCA 
AGTGGCATCC TTAATGTCCT AATCAAACTC TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA 
GCCAAGGAGC AGAAGGAAGT CCAGACCCCA AAGTGGATCA CACCAGTGTT GCTCCTGATT 
GATTTCTATG AAAAGACAGC CATCTCCTCA AAAAGGAGAG CCCAGATGAC TAAGTACCTG 
CAATCCAACA GCAACAACTG GCGCTGGTTT GATGATCGCT CTGGGCGTTG GTGTAGTTAC 
AGTGCAAGCA ACAATAGCAC TATTGATTCT GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA 
TTCACTGCAG GCCGAAGAAG ATACACGGTC CAATTCACTA CAATGGTGCA GGTTAATGAG 
GAAACAGGGA ACCGACGCCC TGTGATGCTG ACTCTCCTCA GGGTACCTCG GCTGAATAAA 
AATTCAAAAA ACAGCAATGG ACAGGAACTA GAGAAGACGC TGGAAGAAAG CAAAGAAATG 
GATATCAAAC GTAAAGAAAA TAAAGGCAAT GATACCCCTT TGGCCCTAGA GAGTACAAAC 
ACTGAAAAGG AGACAAGCCT GGAGGAAACA AAAATCGGGG AGATCCTGAT CCAGGGCTTG 
ACAGAAGATA TGGTGACTGT TTTAATCCGG GCCTGCGTGA GCATGCTGGG AGTCCCTGTG 
GACCCAGATA CTTTGCATGC CACCCTTCGT CTCTGTCTGA GGCTCACCCG GGACCACAAA 
TATGCCATGA TGTTTGCAGA ACTGAAGAGT ACCCGCATGA TCTTGAATTT GACCCAGAGC 
TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC ACCCTTCTCT TAAGACACAT CATTGAGGAC 
CCCTGTACCC TTCGTCATAC CATGGAAAAG GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT 
GGTAGCACTA CCTCTGGTGT TGTGTCTGGC AGCCTCGGCT CTCGGGAGAT CAACTACATC 
CTTCGTGTCC TTGGGCCAGC CGCATGCCGC AATCCAGACA TATTCACAGA AGTGGCCAAC 
TGCTGTATCC GCATCGCCCT TCCTGCCCCT CGAGGCTCAG GAACTGCTTC AGATGATGAA 
TTTGAGAATC TTAGAATTAA AGGCCCTAAT GCTGTACAGC TGGTGAAGAC CACCCCTTTG 
AAGCCCTCAC CTCTGCCTGT CATCCCTGAT ACTATCAAGG AAGTGATCTA TGATATGCTG 
AATGCTCTGG CTGCATACCA TGCTCCAGAG GAAGCAGATA AATCTGATCC TAAACCTGGG 
GTTATGACCC AAGAGGTTGG CCAGCTCCTG CAAGACATGG GTGATGATGT ATACCAGCAG 
TACCGGTCAC TTACGCGTCA GAGCAGTGAC TTTGATACGC AGTCAGGTTT TTCCATTAAT 
AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC ACTGAGACTT CCGCATCTGG GACCTCCCAA 
GGAGAGGCTT CAACTCCAGA GGAGTCTCGA GATGGGAAGA AAGATAAAGA AGGGGACCGG 
GCCTCTGAGG AAGGCAAACA GAAAGGCAAG GGCAGCAAAC CTTTAATGCC TACCTCCACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCC 
AACTACAGCT ACACTGTGGG CCAGTCTGAA CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTTCTGG ACCACCTGCT CCCACATACC CAGAATGCAG AAGACAAGGA CACCCCTGCC 
TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT GCTGCAGGGA GTGGCACAGA TGCCCAGGTG 
GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT GGACGGGCAC TGGCTATGGC TGAGAGTACA 
GAGAAACATG CCAGGCTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAGTCCTGC 
CCCTCCACCT CCAGCTTCTA CAGCAGTGCC ACAGCGAAGA CCCAGCACAA TGGCATGAAC 
AACATCATTC GGCTTTTCCT GAAGAAGGGA CTGGTTAATG ACCTGGCCAG AGTACCTCAC 
AGCTTAGACC TGTCCAGTCC CAACATGGCC AACACAGTCA ATGCTGCTCT GAAGCCTTTG 
GAAACACTTT CCCGGATTGT GAACCAGCCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCCCAA GGAGCCTCTC AAGATTCCAG TAGCAACCAG 
CAGGACCCAG GCGAGCCTGG GGAAGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCTGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAGAATGA GCTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AGCACACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAGG GAGATGAAGA CGATGACGAC GATGGCTCTG AGATGGAATT GGATGAGGAT 
TATCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATCCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCATCC ACTGATGGTG CGCCATGCAG ACCACAGTTC TCTGACACTG 
GGCAGTGGCT CTTCAACAAC TCGTCTCACC CAGGGCATCG GGCGCAGTCA GAGGACCCTA 
AGGCAGCTGA CGGCCAATAC TGGCCACACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCCAACCCTC CTCTTATACT GCAGAGGTTG CTTGGTCCCT CAGCTGCTGC TGACATCCTT 
CAGCTGAGCA GCAGCCTTCC CCTACAAAGC CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC 
GATGACGTCC ACATCATCGC CCGTTCTGAT GATGAGCTGC TGGATGACTT TTTCCATGAT 
CAGAGCACAG CTACCAGCCA AGCAGGAACC CTGTCCAGCA TCCCCACAGC CCTGACCCGC 
TGGACAGAAG AATGCAAAGT TCTCGATGCT GAGAGCATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGTCCA TTGTCAATCA CCTGGAATTC CTGAGGGATG AGGAGCTGGA AGAAAGGCGA 
GAGAAGCGCA GGAAACAACT GGCTGAGGAA GAAACAAAGA TAACTGATAA AGGCAAAGAA 
GATAAGGAGA ACAGGGATCA GAGTGCACAG TGTACTGCAT CTAAGTCAAA TGACTCCACT 
GAACAGAATC TCTCAGATGG GACGCCTATG CCTGACAGCT ACCCAACAAC CCCATCTTCA 
ACTGATGCAG CTACATCTGA GTCCAAGGAG ACCCTTGGCA CTCTGCAATC CTCACAACAG 
CAACCAACAC TCCCAACCCC ACCAGCTTTG GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA 
GCTGGAGAAG GGGGCAGCTC TACACAGCTA TTGATGCCTG TAGAGCCAGA GGAATTGGGT 
CCCACAAGGC CAAGTGGGGA AGCAGAAACA ACTCAGATGG AGTTATCCCC AGCTCCCACT 
ATAACCTCAC TTTCCCCAGA GAGAGCTGAG GATTCTGATG CACTGACGGC TGTCAGCAGT 
CAGCTAGAAG GCTCTCCTAT GGATACAAGC AGCCTGGCTT CCTGTACCTT AGAGGAGGCT 
GTGGGTGACA CTTCAGCAGC TGGCAGTTCT GAGCAGCCCA GAGCAGGCAG CTCCACTCCT 
GGGGATGCCC CACCAGCTGT GGCGGAAGTG CAAGGCAGGA GTGATGGGTC AGGGGAATCT 
GCCCAGCCAC CTGAGGACAG CTCCCCACCT GCATCCTCTG AGAGCTCTTC CACCAGAGAT 
TCTGCCGTGG CCATTTCTGG AGCAGATTCC CGAGGAATCC TAGAAGAGCC GTTGCCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG GGTATCAGTC TCCCTGAAGG TGTGGACCCC 
TCTTTTCTGG CTGCCCTGCC TGATGACATC CGTCGGGAAG TTCTACAGAA CCAGCTAGGC 
ATTCGTCCAC CAACCCGGAC TGCCCCCTCC ACAAATAGCT CAGCGCCTGC AGTGGTGGGG 
AATCCTGGTG TGACTGAAGT GAGCCCTGAG TTTCTGGCTG CCCTGCCTCC AGCCATTCAG 
GAGGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029200.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884689.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X10, mRNA.

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CloneID	OHu99119
Clone ID Related Accession (Same CDS sequence)	XM_017029199.1 , XM_017029201.1 , XM_017029203.1 , XM_017029200.1 , XM_017029202.1 , XM_017029198.1 , XM_017029198.2
Accession Version	XM_017029200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTTTGTT TCTCCTTTTG CAGTGTATTC CAATGCCTTG 
CAGGAATCAG CAAACAGTAT CTTGTATAAT GGCTTGATAG AGGAGTTGGT AGATGTCCTT 
CAGATAACGG ATAAGCAGCT TATGGAGATT AAAGCAGCTT CTTTACGAAC ATTAACATCA 
ATTGTCCACT TGGAGAGAAC TCCCAAACTC AGCAGTATTA TTGACTGTAC TGGAACTGCC 
TCCTACCATG GATTTTTGCC AGTGCTTGTA AGGAACTGTA TCCAGGCCAT GATTGATCCT 
TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG 
GCCAGCTACG ATGCTGGTGG TGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG 
AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCCGTC 
AGAGTGGTTG ACCTTATCAC CAACCTGGAT ATGGCAGCTT TTCAATCCCA TAGTGGACTT 
TCTATCTTCA TTTATAGACT TGAGCATGAA GTAGATTTGT GCCGAAAAGA ATGTCCGTTT 
GTGATCAAGC CAAAGATCCA GAGACCCAAT ACTACACAAG AAGGAGAGGA AATGGAAACT 
GATATGGATG TTGCAGATGT GGCTATGGAA AGCAGTCCAG GCTCATCCAT CTCTATGGAG 
CACAGGCTGG ATGTTGAATT AAGGGCATCA GGTTCCAGCA GCAGCACTAA CATCTCTTCT 
GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC GGCACCGGCC CTGGCCCCGG CCCCGGCCCC 
GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT 
GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT ATTCCACAAC GAGCAGCACT TCTGAAATCC 
ATGTTGAATT TCCTCAAGAA GGCCATCCAA GACCCTGCTT TCTCAGATGG CATACGACAT 
GTGATGGATG GTTCTCTGCC TACCTCCCTG AAACACATCA TCAGCAATGC AGAATACTAT 
GGCCCATCAC TCTTCCTCCT AGCTACTGAA GTGGTGACTG TGTTTGTATT TCAAGAACCA 
TCACTGCTCT CCTCACTCCA GGACAATGGA TTGACAGATG TCATGCTGCA TGCACTGCTT 
ATCAAAGATG TTCCTGCTAC CCGTGAAGTC CTTGGCTCCC TCCCAAATGT ATTCAGTGCA 
CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT TTTGTTCAGT GTCAGCCTTT TGAACGCCTC 
TTCAAAGTTC TTCTGTCTCC AGATTACCTC CCAGCCATGC GGAGGAGGAG AAGTTCTGAT 
CCCCTTGGGG ATACTGCATC CAACCTGGGG AGTGCTGTCG ATGAGCTCAT GAGACATCAG 
CCCACCCTTA AAACAGATGC AACGACTGCC ATCATCAAGT TACTTGAAGA AATCTGTAAT 
CTTGGAAGGG ACCCCAAATA CATCTGTCAG AAGCCATCAA TCCAGAAGGC AGATGGCACT 
GCCACTGCTC CTCCCCCAAG GTCTAATCAT GCCGCAGAAG AAGCCTCTAG TGAGGATGAG 
GAGGAAGAGG AAGTACAGGC CATGCAGAGC TTTAATTCTA CCCAGCAAAA TGAAACTGAG 
CCTAATCAGC AGGTTGTTGG TACAGAGGAA CGTATTCCTA TTCCCCTCAT GGATTACATC 
CTTAATGTGA TGAAATTTGT GGAATCTATT CTGAGCAACA ATACAACAGA TGACCACTGC 
CAGGAATTTG TGAATCAGAA AGGACTGTTG CCTTTGGTTA CCATTTTGGG TCTTCCCAAT 
CTGCCCATTG ACTTTCCCAC ATCTGCTGCC TGTCAGGCTG TTGCAGGTGT CTGCAAATCC 
ATATTGACAC TGTCACATGA ACCCAAAGTC CTTCAAGAGG GTCTCCTTCA GTTGGACTCC 
ATCCTCTCCT CCCTGGAGCC CTTACACCGC CCCATTGAAT CCCCTGGGGG CTCAGTGTTG 
TTGCGAGAAC TGGCTTGCGC AGGCAATGTT GCTGATGCTA CCCTCTCAGC CCAGGCCACA 
CCTCTGCTGC ATGCACTCAC TGCTGCCCAT GCCTACATCA TGATGTTTGT TCATACTTGC 
AGAGTTGGAC AGAGTGAAAT TCGTTCCATC TCCGTAAACC AGTGGGGCTC TCAATTGGGT 
CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA TACTGTTCCC TGGTGTGGGA AAGCACTGTC 
CTCCTCTCTC TGTGTACCCC AAACAGCCTA CCATCTGGGT GTGAATTTGG CCAGGCAGAT 
ATGCAGAAAC TGGTTCCAAA GGATGAGAAG GCAGGTACGA CCCAGGGCGG AAAAAGATCA 
GATGGGGAAC AGGATGGAGC AGCTGGAAGT ATGGATGCTT CTACCCAGGG CTTATTAGAA 
GGCATTGGGC TAGATGGTGA CACATTGGCT CCCATGGAGA CAGATGAACC TACTGCTTCA 
GACTCTAAGG GCAAATCTAA AATCACACCA GCAATGGCTG CCAGAATTAA GCAAATCAAG 
CCTTTGTTAT CAGCTTCCTC CAGATTAGGC CGAGCACTTG CTGAGCTATT TGGACTTCTT 
GTTAAACTTT GTGTGGGATC TCCTGTCCGC CAGAGAAGGA GCCATCATGC TGCCAGCACC 
ACTACAGCAC CGACACCTGC CGCGCGATCA ACAGCCTCAG CTCTCACTAA GCTCTTGACT 
AAGGGGTTAT CTTGGCAGCC CCCACCATAT ACACCTACTC CCCGATTCAG GCTGACATTC 
TTCATCTGTT CAGTTGGTTT CACATCCCCA ATGCTGTTTG ATGAGAGGAA GTATCCCTAC 
CACCTCATGC TGCAAAAATT TCTCTGCTCC GGAGGCCACA ATGCTCTTTT TGAAACTTTC 
AACTGGGCTC TGTCCATGGG AGGTAAAGTT CCTGTTTCTG AGGGATTGGA ACACTCAGAC 
TTGCCTGATG GCACAGGAGA ATTCCTAGAT GCCTGGCTTA TGCTGGTGGA GAAGATGGTG 
AATCCCACCA CGGTGCTTGA ATCTCCACAT TCGCTGCCTG CCAAATTGCC TGGAGGTGTC 
CAGAACTTTC CCCAGTTCAG TGCACTGCGC TTCCTTGTGG TAACTCAGAA AGCAGCCTTT 
ACTTGCATCA AAAACTTATG GAACCGGAAA CCCCTGAAGG TATATGGTGG ACGAATGGCT 
GAATCGATGC TGGCCATTCT ATGCCACATC CTCCGAGGAG AACCTGTGAT TCGAGAGAGA 
CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA GAAGAGGATA CAGGGCAAGA GGAAGGTGGC 
TCCCGCCGGG AACCTCAAGT CAACCAGCAA CAACTGCAAC AGCTCATGGA CATGGGCTTC 
ACAAGGGAAC ATGCAATGGA GGCACTGTTG AACACCAGCA CCATGGAGCA GGCCACAGAG 
TACCTTTTAA CCCACCCTCC TCCAATCATG GGAGGAGTTG TTCGGGATCT CAGCATGTCT 
GAAGAGGACC AGATGATGAG AGCAATTGCT ATGTCTCTGG GACAGGATAT TCCAATGGAT 
CAAAGGGCAG AGTCACCTGA GGAAGTTGCT TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT 
CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA TGTCTAGAGA AGTTCCAGGA TGCTGACCCG 
TTGGAACAAG ATGAGCTCCA CACTTTCACA GATACTATGT TGCCAGGCTG CTTCCACCTT 
CTTGATGAGC TGCCAGACAC AGTATACCGT GTGTGTGACC TGATCATGAC AGCAATCAAA 
CGTAATGGAG CAGATTATCG TGACATGATT CTGAAGCAAG TAGTCAATCA GGTGTGGGAA 
GCTGCTGATG TATTGATCAA AGCTGCTCTT CCCCTGACAA CAAGTGACAC AAAAACCGTG 
TCAGAGTGGA TAAGTCAGAT GGCCACACTG CCCCAGGCCT CCAATTTGGC TACTAGAATC 
TTGCTTTTAA CGCTACTTTT TGAGGAGTTG AAGCTACCTT GTGCTTGGGT GGTTGAATCA 
AGTGGCATCC TTAATGTCCT AATCAAACTC TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA 
GCCAAGGAGC AGAAGGAAGT CCAGACCCCA AAGTGGATCA CACCAGTGTT GCTCCTGATT 
GATTTCTATG AAAAGACAGC CATCTCCTCA AAAAGGAGAG CCCAGATGAC TAAGTACCTG 
CAATCCAACA GCAACAACTG GCGCTGGTTT GATGATCGCT CTGGGCGTTG GTGTAGTTAC 
AGTGCAAGCA ACAATAGCAC TATTGATTCT GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA 
TTCACTGCAG GCCGAAGAAG ATACACGGTC CAATTCACTA CAATGGTGCA GGTTAATGAG 
GAAACAGGGA ACCGACGCCC TGTGATGCTG ACTCTCCTCA GGGTACCTCG GCTGAATAAA 
AATTCAAAAA ACAGCAATGG ACAGGAACTA GAGAAGACGC TGGAAGAAAG CAAAGAAATG 
GATATCAAAC GTAAAGAAAA TAAAGGCAAT GATACCCCTT TGGCCCTAGA GAGTACAAAC 
ACTGAAAAGG AGACAAGCCT GGAGGAAACA AAAATCGGGG AGATCCTGAT CCAGGGCTTG 
ACAGAAGATA TGGTGACTGT TTTAATCCGG GCCTGCGTGA GCATGCTGGG AGTCCCTGTG 
GACCCAGATA CTTTGCATGC CACCCTTCGT CTCTGTCTGA GGCTCACCCG GGACCACAAA 
TATGCCATGA TGTTTGCAGA ACTGAAGAGT ACCCGCATGA TCTTGAATTT GACCCAGAGC 
TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC ACCCTTCTCT TAAGACACAT CATTGAGGAC 
CCCTGTACCC TTCGTCATAC CATGGAAAAG GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT 
GGTAGCACTA CCTCTGGTGT TGTGTCTGGC AGCCTCGGCT CTCGGGAGAT CAACTACATC 
CTTCGTGTCC TTGGGCCAGC CGCATGCCGC AATCCAGACA TATTCACAGA AGTGGCCAAC 
TGCTGTATCC GCATCGCCCT TCCTGCCCCT CGAGGCTCAG GAACTGCTTC AGATGATGAA 
TTTGAGAATC TTAGAATTAA AGGCCCTAAT GCTGTACAGC TGGTGAAGAC CACCCCTTTG 
AAGCCCTCAC CTCTGCCTGT CATCCCTGAT ACTATCAAGG AAGTGATCTA TGATATGCTG 
AATGCTCTGG CTGCATACCA TGCTCCAGAG GAAGCAGATA AATCTGATCC TAAACCTGGG 
GTTATGACCC AAGAGGTTGG CCAGCTCCTG CAAGACATGG GTGATGATGT ATACCAGCAG 
TACCGGTCAC TTACGCGTCA GAGCAGTGAC TTTGATACGC AGTCAGGTTT TTCCATTAAT 
AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC ACTGAGACTT CCGCATCTGG GACCTCCCAA 
GGAGAGGCTT CAACTCCAGA GGAGTCTCGA GATGGGAAGA AAGATAAAGA AGGGGACCGG 
GCCTCTGAGG AAGGCAAACA GAAAGGCAAG GGCAGCAAAC CTTTAATGCC TACCTCCACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCC 
AACTACAGCT ACACTGTGGG CCAGTCTGAA CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTTCTGG ACCACCTGCT CCCACATACC CAGAATGCAG AAGACAAGGA CACCCCTGCC 
TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT GCTGCAGGGA GTGGCACAGA TGCCCAGGTG 
GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT GGACGGGCAC TGGCTATGGC TGAGAGTACA 
GAGAAACATG CCAGGCTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAGTCCTGC 
CCCTCCACCT CCAGCTTCTA CAGCAGTGCC ACAGCGAAGA CCCAGCACAA TGGCATGAAC 
AACATCATTC GGCTTTTCCT GAAGAAGGGA CTGGTTAATG ACCTGGCCAG AGTACCTCAC 
AGCTTAGACC TGTCCAGTCC CAACATGGCC AACACAGTCA ATGCTGCTCT GAAGCCTTTG 
GAAACACTTT CCCGGATTGT GAACCAGCCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCCCAA GGAGCCTCTC AAGATTCCAG TAGCAACCAG 
CAGGACCCAG GCGAGCCTGG GGAAGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCTGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAGAATGA GCTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AGCACACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAGG GAGATGAAGA CGATGACGAC GATGGCTCTG AGATGGAATT GGATGAGGAT 
TATCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATCCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCATCC ACTGATGGTG CGCCATGCAG ACCACAGTTC TCTGACACTG 
GGCAGTGGCT CTTCAACAAC TCGTCTCACC CAGGGCATCG GGCGCAGTCA GAGGACCCTA 
AGGCAGCTGA CGGCCAATAC TGGCCACACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCCAACCCTC CTCTTATACT GCAGAGGTTG CTTGGTCCCT CAGCTGCTGC TGACATCCTT 
CAGCTGAGCA GCAGCCTTCC CCTACAAAGC CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC 
GATGACGTCC ACATCATCGC CCGTTCTGAT GATGAGCTGC TGGATGACTT TTTCCATGAT 
CAGAGCACAG CTACCAGCCA AGCAGGAACC CTGTCCAGCA TCCCCACAGC CCTGACCCGC 
TGGACAGAAG AATGCAAAGT TCTCGATGCT GAGAGCATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGTCCA TTGTCAATCA CCTGGAATTC CTGAGGGATG AGGAGCTGGA AGAAAGGCGA 
GAGAAGCGCA GGAAACAACT GGCTGAGGAA GAAACAAAGA TAACTGATAA AGGCAAAGAA 
GATAAGGAGA ACAGGGATCA GAGTGCACAG TGTACTGCAT CTAAGTCAAA TGACTCCACT 
GAACAGAATC TCTCAGATGG GACGCCTATG CCTGACAGCT ACCCAACAAC CCCATCTTCA 
ACTGATGCAG CTACATCTGA GTCCAAGGAG ACCCTTGGCA CTCTGCAATC CTCACAACAG 
CAACCAACAC TCCCAACCCC ACCAGCTTTG GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA 
GCTGGAGAAG GGGGCAGCTC TACACAGCTA TTGATGCCTG TAGAGCCAGA GGAATTGGGT 
CCCACAAGGC CAAGTGGGGA AGCAGAAACA ACTCAGATGG AGTTATCCCC AGCTCCCACT 
ATAACCTCAC TTTCCCCAGA GAGAGCTGAG GATTCTGATG CACTGACGGC TGTCAGCAGT 
CAGCTAGAAG GCTCTCCTAT GGATACAAGC AGCCTGGCTT CCTGTACCTT AGAGGAGGCT 
GTGGGTGACA CTTCAGCAGC TGGCAGTTCT GAGCAGCCCA GAGCAGGCAG CTCCACTCCT 
GGGGATGCCC CACCAGCTGT GGCGGAAGTG CAAGGCAGGA GTGATGGGTC AGGGGAATCT 
GCCCAGCCAC CTGAGGACAG CTCCCCACCT GCATCCTCTG AGAGCTCTTC CACCAGAGAT 
TCTGCCGTGG CCATTTCTGG AGCAGATTCC CGAGGAATCC TAGAAGAGCC GTTGCCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG GGTATCAGTC TCCCTGAAGG TGTGGACCCC 
TCTTTTCTGG CTGCCCTGCC TGATGACATC CGTCGGGAAG TTCTACAGAA CCAGCTAGGC 
ATTCGTCCAC CAACCCGGAC TGCCCCCTCC ACAAATAGCT CAGCGCCTGC AGTGGTGGGG 
AATCCTGGTG TGACTGAAGT GAGCCCTGAG TTTCTGGCTG CCCTGCCTCC AGCCATTCAG 
GAGGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884689.1
CDS	268..13662
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029200.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029200.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTTTGTT TCTCCTTTTG CAGTGTATTC CAATGCCTTG 
CAGGAATCAG CAAACAGTAT CTTGTATAAT GGCTTGATAG AGGAGTTGGT AGATGTCCTT 
CAGATAACGG ATAAGCAGCT TATGGAGATT AAAGCAGCTT CTTTACGAAC ATTAACATCA 
ATTGTCCACT TGGAGAGAAC TCCCAAACTC AGCAGTATTA TTGACTGTAC TGGAACTGCC 
TCCTACCATG GATTTTTGCC AGTGCTTGTA AGGAACTGTA TCCAGGCCAT GATTGATCCT 
TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG 
GCCAGCTACG ATGCTGGTGG TGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG 
AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCCGTC 
AGAGTGGTTG ACCTTATCAC CAACCTGGAT ATGGCAGCTT TTCAATCCCA TAGTGGACTT 
TCTATCTTCA TTTATAGACT TGAGCATGAA GTAGATTTGT GCCGAAAAGA ATGTCCGTTT 
GTGATCAAGC CAAAGATCCA GAGACCCAAT ACTACACAAG AAGGAGAGGA AATGGAAACT 
GATATGGATG TTGCAGATGT GGCTATGGAA AGCAGTCCAG GCTCATCCAT CTCTATGGAG 
CACAGGCTGG ATGTTGAATT AAGGGCATCA GGTTCCAGCA GCAGCACTAA CATCTCTTCT 
GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC GGCACCGGCC CTGGCCCCGG CCCCGGCCCC 
GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT 
GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT ATTCCACAAC GAGCAGCACT TCTGAAATCC 
ATGTTGAATT TCCTCAAGAA GGCCATCCAA GACCCTGCTT TCTCAGATGG CATACGACAT 
GTGATGGATG GTTCTCTGCC TACCTCCCTG AAACACATCA TCAGCAATGC AGAATACTAT 
GGCCCATCAC TCTTCCTCCT AGCTACTGAA GTGGTGACTG TGTTTGTATT TCAAGAACCA 
TCACTGCTCT CCTCACTCCA GGACAATGGA TTGACAGATG TCATGCTGCA TGCACTGCTT 
ATCAAAGATG TTCCTGCTAC CCGTGAAGTC CTTGGCTCCC TCCCAAATGT ATTCAGTGCA 
CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT TTTGTTCAGT GTCAGCCTTT TGAACGCCTC 
TTCAAAGTTC TTCTGTCTCC AGATTACCTC CCAGCCATGC GGAGGAGGAG AAGTTCTGAT 
CCCCTTGGGG ATACTGCATC CAACCTGGGG AGTGCTGTCG ATGAGCTCAT GAGACATCAG 
CCCACCCTTA AAACAGATGC AACGACTGCC ATCATCAAGT TACTTGAAGA AATCTGTAAT 
CTTGGAAGGG ACCCCAAATA CATCTGTCAG AAGCCATCAA TCCAGAAGGC AGATGGCACT 
GCCACTGCTC CTCCCCCAAG GTCTAATCAT GCCGCAGAAG AAGCCTCTAG TGAGGATGAG 
GAGGAAGAGG AAGTACAGGC CATGCAGAGC TTTAATTCTA CCCAGCAAAA TGAAACTGAG 
CCTAATCAGC AGGTTGTTGG TACAGAGGAA CGTATTCCTA TTCCCCTCAT GGATTACATC 
CTTAATGTGA TGAAATTTGT GGAATCTATT CTGAGCAACA ATACAACAGA TGACCACTGC 
CAGGAATTTG TGAATCAGAA AGGACTGTTG CCTTTGGTTA CCATTTTGGG TCTTCCCAAT 
CTGCCCATTG ACTTTCCCAC ATCTGCTGCC TGTCAGGCTG TTGCAGGTGT CTGCAAATCC 
ATATTGACAC TGTCACATGA ACCCAAAGTC CTTCAAGAGG GTCTCCTTCA GTTGGACTCC 
ATCCTCTCCT CCCTGGAGCC CTTACACCGC CCCATTGAAT CCCCTGGGGG CTCAGTGTTG 
TTGCGAGAAC TGGCTTGCGC AGGCAATGTT GCTGATGCTA CCCTCTCAGC CCAGGCCACA 
CCTCTGCTGC ATGCACTCAC TGCTGCCCAT GCCTACATCA TGATGTTTGT TCATACTTGC 
AGAGTTGGAC AGAGTGAAAT TCGTTCCATC TCCGTAAACC AGTGGGGCTC TCAATTGGGT 
CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA TACTGTTCCC TGGTGTGGGA AAGCACTGTC 
CTCCTCTCTC TGTGTACCCC AAACAGCCTA CCATCTGGGT GTGAATTTGG CCAGGCAGAT 
ATGCAGAAAC TGGTTCCAAA GGATGAGAAG GCAGGTACGA CCCAGGGCGG AAAAAGATCA 
GATGGGGAAC AGGATGGAGC AGCTGGAAGT ATGGATGCTT CTACCCAGGG CTTATTAGAA 
GGCATTGGGC TAGATGGTGA CACATTGGCT CCCATGGAGA CAGATGAACC TACTGCTTCA 
GACTCTAAGG GCAAATCTAA AATCACACCA GCAATGGCTG CCAGAATTAA GCAAATCAAG 
CCTTTGTTAT CAGCTTCCTC CAGATTAGGC CGAGCACTTG CTGAGCTATT TGGACTTCTT 
GTTAAACTTT GTGTGGGATC TCCTGTCCGC CAGAGAAGGA GCCATCATGC TGCCAGCACC 
ACTACAGCAC CGACACCTGC CGCGCGATCA ACAGCCTCAG CTCTCACTAA GCTCTTGACT 
AAGGGGTTAT CTTGGCAGCC CCCACCATAT ACACCTACTC CCCGATTCAG GCTGACATTC 
TTCATCTGTT CAGTTGGTTT CACATCCCCA ATGCTGTTTG ATGAGAGGAA GTATCCCTAC 
CACCTCATGC TGCAAAAATT TCTCTGCTCC GGAGGCCACA ATGCTCTTTT TGAAACTTTC 
AACTGGGCTC TGTCCATGGG AGGTAAAGTT CCTGTTTCTG AGGGATTGGA ACACTCAGAC 
TTGCCTGATG GCACAGGAGA ATTCCTAGAT GCCTGGCTTA TGCTGGTGGA GAAGATGGTG 
AATCCCACCA CGGTGCTTGA ATCTCCACAT TCGCTGCCTG CCAAATTGCC TGGAGGTGTC 
CAGAACTTTC CCCAGTTCAG TGCACTGCGC TTCCTTGTGG TAACTCAGAA AGCAGCCTTT 
ACTTGCATCA AAAACTTATG GAACCGGAAA CCCCTGAAGG TATATGGTGG ACGAATGGCT 
GAATCGATGC TGGCCATTCT ATGCCACATC CTCCGAGGAG AACCTGTGAT TCGAGAGAGA 
CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA GAAGAGGATA CAGGGCAAGA GGAAGGTGGC 
TCCCGCCGGG AACCTCAAGT CAACCAGCAA CAACTGCAAC AGCTCATGGA CATGGGCTTC 
ACAAGGGAAC ATGCAATGGA GGCACTGTTG AACACCAGCA CCATGGAGCA GGCCACAGAG 
TACCTTTTAA CCCACCCTCC TCCAATCATG GGAGGAGTTG TTCGGGATCT CAGCATGTCT 
GAAGAGGACC AGATGATGAG AGCAATTGCT ATGTCTCTGG GACAGGATAT TCCAATGGAT 
CAAAGGGCAG AGTCACCTGA GGAAGTTGCT TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT 
CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA TGTCTAGAGA AGTTCCAGGA TGCTGACCCG 
TTGGAACAAG ATGAGCTCCA CACTTTCACA GATACTATGT TGCCAGGCTG CTTCCACCTT 
CTTGATGAGC TGCCAGACAC AGTATACCGT GTGTGTGACC TGATCATGAC AGCAATCAAA 
CGTAATGGAG CAGATTATCG TGACATGATT CTGAAGCAAG TAGTCAATCA GGTGTGGGAA 
GCTGCTGATG TATTGATCAA AGCTGCTCTT CCCCTGACAA CAAGTGACAC AAAAACCGTG 
TCAGAGTGGA TAAGTCAGAT GGCCACACTG CCCCAGGCCT CCAATTTGGC TACTAGAATC 
TTGCTTTTAA CGCTACTTTT TGAGGAGTTG AAGCTACCTT GTGCTTGGGT GGTTGAATCA 
AGTGGCATCC TTAATGTCCT AATCAAACTC TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA 
GCCAAGGAGC AGAAGGAAGT CCAGACCCCA AAGTGGATCA CACCAGTGTT GCTCCTGATT 
GATTTCTATG AAAAGACAGC CATCTCCTCA AAAAGGAGAG CCCAGATGAC TAAGTACCTG 
CAATCCAACA GCAACAACTG GCGCTGGTTT GATGATCGCT CTGGGCGTTG GTGTAGTTAC 
AGTGCAAGCA ACAATAGCAC TATTGATTCT GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA 
TTCACTGCAG GCCGAAGAAG ATACACGGTC CAATTCACTA CAATGGTGCA GGTTAATGAG 
GAAACAGGGA ACCGACGCCC TGTGATGCTG ACTCTCCTCA GGGTACCTCG GCTGAATAAA 
AATTCAAAAA ACAGCAATGG ACAGGAACTA GAGAAGACGC TGGAAGAAAG CAAAGAAATG 
GATATCAAAC GTAAAGAAAA TAAAGGCAAT GATACCCCTT TGGCCCTAGA GAGTACAAAC 
ACTGAAAAGG AGACAAGCCT GGAGGAAACA AAAATCGGGG AGATCCTGAT CCAGGGCTTG 
ACAGAAGATA TGGTGACTGT TTTAATCCGG GCCTGCGTGA GCATGCTGGG AGTCCCTGTG 
GACCCAGATA CTTTGCATGC CACCCTTCGT CTCTGTCTGA GGCTCACCCG GGACCACAAA 
TATGCCATGA TGTTTGCAGA ACTGAAGAGT ACCCGCATGA TCTTGAATTT GACCCAGAGC 
TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC ACCCTTCTCT TAAGACACAT CATTGAGGAC 
CCCTGTACCC TTCGTCATAC CATGGAAAAG GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT 
GGTAGCACTA CCTCTGGTGT TGTGTCTGGC AGCCTCGGCT CTCGGGAGAT CAACTACATC 
CTTCGTGTCC TTGGGCCAGC CGCATGCCGC AATCCAGACA TATTCACAGA AGTGGCCAAC 
TGCTGTATCC GCATCGCCCT TCCTGCCCCT CGAGGCTCAG GAACTGCTTC AGATGATGAA 
TTTGAGAATC TTAGAATTAA AGGCCCTAAT GCTGTACAGC TGGTGAAGAC CACCCCTTTG 
AAGCCCTCAC CTCTGCCTGT CATCCCTGAT ACTATCAAGG AAGTGATCTA TGATATGCTG 
AATGCTCTGG CTGCATACCA TGCTCCAGAG GAAGCAGATA AATCTGATCC TAAACCTGGG 
GTTATGACCC AAGAGGTTGG CCAGCTCCTG CAAGACATGG GTGATGATGT ATACCAGCAG 
TACCGGTCAC TTACGCGTCA GAGCAGTGAC TTTGATACGC AGTCAGGTTT TTCCATTAAT 
AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC ACTGAGACTT CCGCATCTGG GACCTCCCAA 
GGAGAGGCTT CAACTCCAGA GGAGTCTCGA GATGGGAAGA AAGATAAAGA AGGGGACCGG 
GCCTCTGAGG AAGGCAAACA GAAAGGCAAG GGCAGCAAAC CTTTAATGCC TACCTCCACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCC 
AACTACAGCT ACACTGTGGG CCAGTCTGAA CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTTCTGG ACCACCTGCT CCCACATACC CAGAATGCAG AAGACAAGGA CACCCCTGCC 
TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT GCTGCAGGGA GTGGCACAGA TGCCCAGGTG 
GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT GGACGGGCAC TGGCTATGGC TGAGAGTACA 
GAGAAACATG CCAGGCTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAGTCCTGC 
CCCTCCACCT CCAGCTTCTA CAGCAGTGCC ACAGCGAAGA CCCAGCACAA TGGCATGAAC 
AACATCATTC GGCTTTTCCT GAAGAAGGGA CTGGTTAATG ACCTGGCCAG AGTACCTCAC 
AGCTTAGACC TGTCCAGTCC CAACATGGCC AACACAGTCA ATGCTGCTCT GAAGCCTTTG 
GAAACACTTT CCCGGATTGT GAACCAGCCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCCCAA GGAGCCTCTC AAGATTCCAG TAGCAACCAG 
CAGGACCCAG GCGAGCCTGG GGAAGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCTGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAGAATGA GCTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AGCACACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAGG GAGATGAAGA CGATGACGAC GATGGCTCTG AGATGGAATT GGATGAGGAT 
TATCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATCCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCATCC ACTGATGGTG CGCCATGCAG ACCACAGTTC TCTGACACTG 
GGCAGTGGCT CTTCAACAAC TCGTCTCACC CAGGGCATCG GGCGCAGTCA GAGGACCCTA 
AGGCAGCTGA CGGCCAATAC TGGCCACACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCCAACCCTC CTCTTATACT GCAGAGGTTG CTTGGTCCCT CAGCTGCTGC TGACATCCTT 
CAGCTGAGCA GCAGCCTTCC CCTACAAAGC CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC 
GATGACGTCC ACATCATCGC CCGTTCTGAT GATGAGCTGC TGGATGACTT TTTCCATGAT 
CAGAGCACAG CTACCAGCCA AGCAGGAACC CTGTCCAGCA TCCCCACAGC CCTGACCCGC 
TGGACAGAAG AATGCAAAGT TCTCGATGCT GAGAGCATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGTCCA TTGTCAATCA CCTGGAATTC CTGAGGGATG AGGAGCTGGA AGAAAGGCGA 
GAGAAGCGCA GGAAACAACT GGCTGAGGAA GAAACAAAGA TAACTGATAA AGGCAAAGAA 
GATAAGGAGA ACAGGGATCA GAGTGCACAG TGTACTGCAT CTAAGTCAAA TGACTCCACT 
GAACAGAATC TCTCAGATGG GACGCCTATG CCTGACAGCT ACCCAACAAC CCCATCTTCA 
ACTGATGCAG CTACATCTGA GTCCAAGGAG ACCCTTGGCA CTCTGCAATC CTCACAACAG 
CAACCAACAC TCCCAACCCC ACCAGCTTTG GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA 
GCTGGAGAAG GGGGCAGCTC TACACAGCTA TTGATGCCTG TAGAGCCAGA GGAATTGGGT 
CCCACAAGGC CAAGTGGGGA AGCAGAAACA ACTCAGATGG AGTTATCCCC AGCTCCCACT 
ATAACCTCAC TTTCCCCAGA GAGAGCTGAG GATTCTGATG CACTGACGGC TGTCAGCAGT 
CAGCTAGAAG GCTCTCCTAT GGATACAAGC AGCCTGGCTT CCTGTACCTT AGAGGAGGCT 
GTGGGTGACA CTTCAGCAGC TGGCAGTTCT GAGCAGCCCA GAGCAGGCAG CTCCACTCCT 
GGGGATGCCC CACCAGCTGT GGCGGAAGTG CAAGGCAGGA GTGATGGGTC AGGGGAATCT 
GCCCAGCCAC CTGAGGACAG CTCCCCACCT GCATCCTCTG AGAGCTCTTC CACCAGAGAT 
TCTGCCGTGG CCATTTCTGG AGCAGATTCC CGAGGAATCC TAGAAGAGCC GTTGCCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG GGTATCAGTC TCCCTGAAGG TGTGGACCCC 
TCTTTTCTGG CTGCCCTGCC TGATGACATC CGTCGGGAAG TTCTACAGAA CCAGCTAGGC 
ATTCGTCCAC CAACCCGGAC TGCCCCCTCC ACAAATAGCT CAGCGCCTGC AGTGGTGGGG 
AATCCTGGTG TGACTGAAGT GAGCCCTGAG TTTCTGGCTG CCCTGCCTCC AGCCATTCAG 
GAGGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029202.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884691.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X12, mRNA.

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CloneID	OHu99119
Clone ID Related Accession (Same CDS sequence)	XM_017029199.1 , XM_017029201.1 , XM_017029203.1 , XM_017029200.1 , XM_017029202.1 , XM_017029198.1 , XM_017029198.2
Accession Version	XM_017029202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTTTGTT TCTCCTTTTG CAGTGTATTC CAATGCCTTG 
CAGGAATCAG CAAACAGTAT CTTGTATAAT GGCTTGATAG AGGAGTTGGT AGATGTCCTT 
CAGATAACGG ATAAGCAGCT TATGGAGATT AAAGCAGCTT CTTTACGAAC ATTAACATCA 
ATTGTCCACT TGGAGAGAAC TCCCAAACTC AGCAGTATTA TTGACTGTAC TGGAACTGCC 
TCCTACCATG GATTTTTGCC AGTGCTTGTA AGGAACTGTA TCCAGGCCAT GATTGATCCT 
TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG 
GCCAGCTACG ATGCTGGTGG TGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG 
AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCCGTC 
AGAGTGGTTG ACCTTATCAC CAACCTGGAT ATGGCAGCTT TTCAATCCCA TAGTGGACTT 
TCTATCTTCA TTTATAGACT TGAGCATGAA GTAGATTTGT GCCGAAAAGA ATGTCCGTTT 
GTGATCAAGC CAAAGATCCA GAGACCCAAT ACTACACAAG AAGGAGAGGA AATGGAAACT 
GATATGGATG TTGCAGATGT GGCTATGGAA AGCAGTCCAG GCTCATCCAT CTCTATGGAG 
CACAGGCTGG ATGTTGAATT AAGGGCATCA GGTTCCAGCA GCAGCACTAA CATCTCTTCT 
GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC GGCACCGGCC CTGGCCCCGG CCCCGGCCCC 
GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT 
GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT ATTCCACAAC GAGCAGCACT TCTGAAATCC 
ATGTTGAATT TCCTCAAGAA GGCCATCCAA GACCCTGCTT TCTCAGATGG CATACGACAT 
GTGATGGATG GTTCTCTGCC TACCTCCCTG AAACACATCA TCAGCAATGC AGAATACTAT 
GGCCCATCAC TCTTCCTCCT AGCTACTGAA GTGGTGACTG TGTTTGTATT TCAAGAACCA 
TCACTGCTCT CCTCACTCCA GGACAATGGA TTGACAGATG TCATGCTGCA TGCACTGCTT 
ATCAAAGATG TTCCTGCTAC CCGTGAAGTC CTTGGCTCCC TCCCAAATGT ATTCAGTGCA 
CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT TTTGTTCAGT GTCAGCCTTT TGAACGCCTC 
TTCAAAGTTC TTCTGTCTCC AGATTACCTC CCAGCCATGC GGAGGAGGAG AAGTTCTGAT 
CCCCTTGGGG ATACTGCATC CAACCTGGGG AGTGCTGTCG ATGAGCTCAT GAGACATCAG 
CCCACCCTTA AAACAGATGC AACGACTGCC ATCATCAAGT TACTTGAAGA AATCTGTAAT 
CTTGGAAGGG ACCCCAAATA CATCTGTCAG AAGCCATCAA TCCAGAAGGC AGATGGCACT 
GCCACTGCTC CTCCCCCAAG GTCTAATCAT GCCGCAGAAG AAGCCTCTAG TGAGGATGAG 
GAGGAAGAGG AAGTACAGGC CATGCAGAGC TTTAATTCTA CCCAGCAAAA TGAAACTGAG 
CCTAATCAGC AGGTTGTTGG TACAGAGGAA CGTATTCCTA TTCCCCTCAT GGATTACATC 
CTTAATGTGA TGAAATTTGT GGAATCTATT CTGAGCAACA ATACAACAGA TGACCACTGC 
CAGGAATTTG TGAATCAGAA AGGACTGTTG CCTTTGGTTA CCATTTTGGG TCTTCCCAAT 
CTGCCCATTG ACTTTCCCAC ATCTGCTGCC TGTCAGGCTG TTGCAGGTGT CTGCAAATCC 
ATATTGACAC TGTCACATGA ACCCAAAGTC CTTCAAGAGG GTCTCCTTCA GTTGGACTCC 
ATCCTCTCCT CCCTGGAGCC CTTACACCGC CCCATTGAAT CCCCTGGGGG CTCAGTGTTG 
TTGCGAGAAC TGGCTTGCGC AGGCAATGTT GCTGATGCTA CCCTCTCAGC CCAGGCCACA 
CCTCTGCTGC ATGCACTCAC TGCTGCCCAT GCCTACATCA TGATGTTTGT TCATACTTGC 
AGAGTTGGAC AGAGTGAAAT TCGTTCCATC TCCGTAAACC AGTGGGGCTC TCAATTGGGT 
CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA TACTGTTCCC TGGTGTGGGA AAGCACTGTC 
CTCCTCTCTC TGTGTACCCC AAACAGCCTA CCATCTGGGT GTGAATTTGG CCAGGCAGAT 
ATGCAGAAAC TGGTTCCAAA GGATGAGAAG GCAGGTACGA CCCAGGGCGG AAAAAGATCA 
GATGGGGAAC AGGATGGAGC AGCTGGAAGT ATGGATGCTT CTACCCAGGG CTTATTAGAA 
GGCATTGGGC TAGATGGTGA CACATTGGCT CCCATGGAGA CAGATGAACC TACTGCTTCA 
GACTCTAAGG GCAAATCTAA AATCACACCA GCAATGGCTG CCAGAATTAA GCAAATCAAG 
CCTTTGTTAT CAGCTTCCTC CAGATTAGGC CGAGCACTTG CTGAGCTATT TGGACTTCTT 
GTTAAACTTT GTGTGGGATC TCCTGTCCGC CAGAGAAGGA GCCATCATGC TGCCAGCACC 
ACTACAGCAC CGACACCTGC CGCGCGATCA ACAGCCTCAG CTCTCACTAA GCTCTTGACT 
AAGGGGTTAT CTTGGCAGCC CCCACCATAT ACACCTACTC CCCGATTCAG GCTGACATTC 
TTCATCTGTT CAGTTGGTTT CACATCCCCA ATGCTGTTTG ATGAGAGGAA GTATCCCTAC 
CACCTCATGC TGCAAAAATT TCTCTGCTCC GGAGGCCACA ATGCTCTTTT TGAAACTTTC 
AACTGGGCTC TGTCCATGGG AGGTAAAGTT CCTGTTTCTG AGGGATTGGA ACACTCAGAC 
TTGCCTGATG GCACAGGAGA ATTCCTAGAT GCCTGGCTTA TGCTGGTGGA GAAGATGGTG 
AATCCCACCA CGGTGCTTGA ATCTCCACAT TCGCTGCCTG CCAAATTGCC TGGAGGTGTC 
CAGAACTTTC CCCAGTTCAG TGCACTGCGC TTCCTTGTGG TAACTCAGAA AGCAGCCTTT 
ACTTGCATCA AAAACTTATG GAACCGGAAA CCCCTGAAGG TATATGGTGG ACGAATGGCT 
GAATCGATGC TGGCCATTCT ATGCCACATC CTCCGAGGAG AACCTGTGAT TCGAGAGAGA 
CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA GAAGAGGATA CAGGGCAAGA GGAAGGTGGC 
TCCCGCCGGG AACCTCAAGT CAACCAGCAA CAACTGCAAC AGCTCATGGA CATGGGCTTC 
ACAAGGGAAC ATGCAATGGA GGCACTGTTG AACACCAGCA CCATGGAGCA GGCCACAGAG 
TACCTTTTAA CCCACCCTCC TCCAATCATG GGAGGAGTTG TTCGGGATCT CAGCATGTCT 
GAAGAGGACC AGATGATGAG AGCAATTGCT ATGTCTCTGG GACAGGATAT TCCAATGGAT 
CAAAGGGCAG AGTCACCTGA GGAAGTTGCT TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT 
CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA TGTCTAGAGA AGTTCCAGGA TGCTGACCCG 
TTGGAACAAG ATGAGCTCCA CACTTTCACA GATACTATGT TGCCAGGCTG CTTCCACCTT 
CTTGATGAGC TGCCAGACAC AGTATACCGT GTGTGTGACC TGATCATGAC AGCAATCAAA 
CGTAATGGAG CAGATTATCG TGACATGATT CTGAAGCAAG TAGTCAATCA GGTGTGGGAA 
GCTGCTGATG TATTGATCAA AGCTGCTCTT CCCCTGACAA CAAGTGACAC AAAAACCGTG 
TCAGAGTGGA TAAGTCAGAT GGCCACACTG CCCCAGGCCT CCAATTTGGC TACTAGAATC 
TTGCTTTTAA CGCTACTTTT TGAGGAGTTG AAGCTACCTT GTGCTTGGGT GGTTGAATCA 
AGTGGCATCC TTAATGTCCT AATCAAACTC TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA 
GCCAAGGAGC AGAAGGAAGT CCAGACCCCA AAGTGGATCA CACCAGTGTT GCTCCTGATT 
GATTTCTATG AAAAGACAGC CATCTCCTCA AAAAGGAGAG CCCAGATGAC TAAGTACCTG 
CAATCCAACA GCAACAACTG GCGCTGGTTT GATGATCGCT CTGGGCGTTG GTGTAGTTAC 
AGTGCAAGCA ACAATAGCAC TATTGATTCT GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA 
TTCACTGCAG GCCGAAGAAG ATACACGGTC CAATTCACTA CAATGGTGCA GGTTAATGAG 
GAAACAGGGA ACCGACGCCC TGTGATGCTG ACTCTCCTCA GGGTACCTCG GCTGAATAAA 
AATTCAAAAA ACAGCAATGG ACAGGAACTA GAGAAGACGC TGGAAGAAAG CAAAGAAATG 
GATATCAAAC GTAAAGAAAA TAAAGGCAAT GATACCCCTT TGGCCCTAGA GAGTACAAAC 
ACTGAAAAGG AGACAAGCCT GGAGGAAACA AAAATCGGGG AGATCCTGAT CCAGGGCTTG 
ACAGAAGATA TGGTGACTGT TTTAATCCGG GCCTGCGTGA GCATGCTGGG AGTCCCTGTG 
GACCCAGATA CTTTGCATGC CACCCTTCGT CTCTGTCTGA GGCTCACCCG GGACCACAAA 
TATGCCATGA TGTTTGCAGA ACTGAAGAGT ACCCGCATGA TCTTGAATTT GACCCAGAGC 
TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC ACCCTTCTCT TAAGACACAT CATTGAGGAC 
CCCTGTACCC TTCGTCATAC CATGGAAAAG GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT 
GGTAGCACTA CCTCTGGTGT TGTGTCTGGC AGCCTCGGCT CTCGGGAGAT CAACTACATC 
CTTCGTGTCC TTGGGCCAGC CGCATGCCGC AATCCAGACA TATTCACAGA AGTGGCCAAC 
TGCTGTATCC GCATCGCCCT TCCTGCCCCT CGAGGCTCAG GAACTGCTTC AGATGATGAA 
TTTGAGAATC TTAGAATTAA AGGCCCTAAT GCTGTACAGC TGGTGAAGAC CACCCCTTTG 
AAGCCCTCAC CTCTGCCTGT CATCCCTGAT ACTATCAAGG AAGTGATCTA TGATATGCTG 
AATGCTCTGG CTGCATACCA TGCTCCAGAG GAAGCAGATA AATCTGATCC TAAACCTGGG 
GTTATGACCC AAGAGGTTGG CCAGCTCCTG CAAGACATGG GTGATGATGT ATACCAGCAG 
TACCGGTCAC TTACGCGTCA GAGCAGTGAC TTTGATACGC AGTCAGGTTT TTCCATTAAT 
AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC ACTGAGACTT CCGCATCTGG GACCTCCCAA 
GGAGAGGCTT CAACTCCAGA GGAGTCTCGA GATGGGAAGA AAGATAAAGA AGGGGACCGG 
GCCTCTGAGG AAGGCAAACA GAAAGGCAAG GGCAGCAAAC CTTTAATGCC TACCTCCACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCC 
AACTACAGCT ACACTGTGGG CCAGTCTGAA CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTTCTGG ACCACCTGCT CCCACATACC CAGAATGCAG AAGACAAGGA CACCCCTGCC 
TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT GCTGCAGGGA GTGGCACAGA TGCCCAGGTG 
GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT GGACGGGCAC TGGCTATGGC TGAGAGTACA 
GAGAAACATG CCAGGCTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAGTCCTGC 
CCCTCCACCT CCAGCTTCTA CAGCAGTGCC ACAGCGAAGA CCCAGCACAA TGGCATGAAC 
AACATCATTC GGCTTTTCCT GAAGAAGGGA CTGGTTAATG ACCTGGCCAG AGTACCTCAC 
AGCTTAGACC TGTCCAGTCC CAACATGGCC AACACAGTCA ATGCTGCTCT GAAGCCTTTG 
GAAACACTTT CCCGGATTGT GAACCAGCCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCCCAA GGAGCCTCTC AAGATTCCAG TAGCAACCAG 
CAGGACCCAG GCGAGCCTGG GGAAGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCTGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAGAATGA GCTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AGCACACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAGG GAGATGAAGA CGATGACGAC GATGGCTCTG AGATGGAATT GGATGAGGAT 
TATCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATCCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCATCC ACTGATGGTG CGCCATGCAG ACCACAGTTC TCTGACACTG 
GGCAGTGGCT CTTCAACAAC TCGTCTCACC CAGGGCATCG GGCGCAGTCA GAGGACCCTA 
AGGCAGCTGA CGGCCAATAC TGGCCACACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCCAACCCTC CTCTTATACT GCAGAGGTTG CTTGGTCCCT CAGCTGCTGC TGACATCCTT 
CAGCTGAGCA GCAGCCTTCC CCTACAAAGC CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC 
GATGACGTCC ACATCATCGC CCGTTCTGAT GATGAGCTGC TGGATGACTT TTTCCATGAT 
CAGAGCACAG CTACCAGCCA AGCAGGAACC CTGTCCAGCA TCCCCACAGC CCTGACCCGC 
TGGACAGAAG AATGCAAAGT TCTCGATGCT GAGAGCATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGTCCA TTGTCAATCA CCTGGAATTC CTGAGGGATG AGGAGCTGGA AGAAAGGCGA 
GAGAAGCGCA GGAAACAACT GGCTGAGGAA GAAACAAAGA TAACTGATAA AGGCAAAGAA 
GATAAGGAGA ACAGGGATCA GAGTGCACAG TGTACTGCAT CTAAGTCAAA TGACTCCACT 
GAACAGAATC TCTCAGATGG GACGCCTATG CCTGACAGCT ACCCAACAAC CCCATCTTCA 
ACTGATGCAG CTACATCTGA GTCCAAGGAG ACCCTTGGCA CTCTGCAATC CTCACAACAG 
CAACCAACAC TCCCAACCCC ACCAGCTTTG GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA 
GCTGGAGAAG GGGGCAGCTC TACACAGCTA TTGATGCCTG TAGAGCCAGA GGAATTGGGT 
CCCACAAGGC CAAGTGGGGA AGCAGAAACA ACTCAGATGG AGTTATCCCC AGCTCCCACT 
ATAACCTCAC TTTCCCCAGA GAGAGCTGAG GATTCTGATG CACTGACGGC TGTCAGCAGT 
CAGCTAGAAG GCTCTCCTAT GGATACAAGC AGCCTGGCTT CCTGTACCTT AGAGGAGGCT 
GTGGGTGACA CTTCAGCAGC TGGCAGTTCT GAGCAGCCCA GAGCAGGCAG CTCCACTCCT 
GGGGATGCCC CACCAGCTGT GGCGGAAGTG CAAGGCAGGA GTGATGGGTC AGGGGAATCT 
GCCCAGCCAC CTGAGGACAG CTCCCCACCT GCATCCTCTG AGAGCTCTTC CACCAGAGAT 
TCTGCCGTGG CCATTTCTGG AGCAGATTCC CGAGGAATCC TAGAAGAGCC GTTGCCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG GGTATCAGTC TCCCTGAAGG TGTGGACCCC 
TCTTTTCTGG CTGCCCTGCC TGATGACATC CGTCGGGAAG TTCTACAGAA CCAGCTAGGC 
ATTCGTCCAC CAACCCGGAC TGCCCCCTCC ACAAATAGCT CAGCGCCTGC AGTGGTGGGG 
AATCCTGGTG TGACTGAAGT GAGCCCTGAG TTTCTGGCTG CCCTGCCTCC AGCCATTCAG 
GAGGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884691.1
CDS	261..13655
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029202.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029202.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTTTGTT TCTCCTTTTG CAGTGTATTC CAATGCCTTG 
CAGGAATCAG CAAACAGTAT CTTGTATAAT GGCTTGATAG AGGAGTTGGT AGATGTCCTT 
CAGATAACGG ATAAGCAGCT TATGGAGATT AAAGCAGCTT CTTTACGAAC ATTAACATCA 
ATTGTCCACT TGGAGAGAAC TCCCAAACTC AGCAGTATTA TTGACTGTAC TGGAACTGCC 
TCCTACCATG GATTTTTGCC AGTGCTTGTA AGGAACTGTA TCCAGGCCAT GATTGATCCT 
TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG 
GCCAGCTACG ATGCTGGTGG TGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG 
AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCCGTC 
AGAGTGGTTG ACCTTATCAC CAACCTGGAT ATGGCAGCTT TTCAATCCCA TAGTGGACTT 
TCTATCTTCA TTTATAGACT TGAGCATGAA GTAGATTTGT GCCGAAAAGA ATGTCCGTTT 
GTGATCAAGC CAAAGATCCA GAGACCCAAT ACTACACAAG AAGGAGAGGA AATGGAAACT 
GATATGGATG TTGCAGATGT GGCTATGGAA AGCAGTCCAG GCTCATCCAT CTCTATGGAG 
CACAGGCTGG ATGTTGAATT AAGGGCATCA GGTTCCAGCA GCAGCACTAA CATCTCTTCT 
GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC GGCACCGGCC CTGGCCCCGG CCCCGGCCCC 
GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT 
GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT ATTCCACAAC GAGCAGCACT TCTGAAATCC 
ATGTTGAATT TCCTCAAGAA GGCCATCCAA GACCCTGCTT TCTCAGATGG CATACGACAT 
GTGATGGATG GTTCTCTGCC TACCTCCCTG AAACACATCA TCAGCAATGC AGAATACTAT 
GGCCCATCAC TCTTCCTCCT AGCTACTGAA GTGGTGACTG TGTTTGTATT TCAAGAACCA 
TCACTGCTCT CCTCACTCCA GGACAATGGA TTGACAGATG TCATGCTGCA TGCACTGCTT 
ATCAAAGATG TTCCTGCTAC CCGTGAAGTC CTTGGCTCCC TCCCAAATGT ATTCAGTGCA 
CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT TTTGTTCAGT GTCAGCCTTT TGAACGCCTC 
TTCAAAGTTC TTCTGTCTCC AGATTACCTC CCAGCCATGC GGAGGAGGAG AAGTTCTGAT 
CCCCTTGGGG ATACTGCATC CAACCTGGGG AGTGCTGTCG ATGAGCTCAT GAGACATCAG 
CCCACCCTTA AAACAGATGC AACGACTGCC ATCATCAAGT TACTTGAAGA AATCTGTAAT 
CTTGGAAGGG ACCCCAAATA CATCTGTCAG AAGCCATCAA TCCAGAAGGC AGATGGCACT 
GCCACTGCTC CTCCCCCAAG GTCTAATCAT GCCGCAGAAG AAGCCTCTAG TGAGGATGAG 
GAGGAAGAGG AAGTACAGGC CATGCAGAGC TTTAATTCTA CCCAGCAAAA TGAAACTGAG 
CCTAATCAGC AGGTTGTTGG TACAGAGGAA CGTATTCCTA TTCCCCTCAT GGATTACATC 
CTTAATGTGA TGAAATTTGT GGAATCTATT CTGAGCAACA ATACAACAGA TGACCACTGC 
CAGGAATTTG TGAATCAGAA AGGACTGTTG CCTTTGGTTA CCATTTTGGG TCTTCCCAAT 
CTGCCCATTG ACTTTCCCAC ATCTGCTGCC TGTCAGGCTG TTGCAGGTGT CTGCAAATCC 
ATATTGACAC TGTCACATGA ACCCAAAGTC CTTCAAGAGG GTCTCCTTCA GTTGGACTCC 
ATCCTCTCCT CCCTGGAGCC CTTACACCGC CCCATTGAAT CCCCTGGGGG CTCAGTGTTG 
TTGCGAGAAC TGGCTTGCGC AGGCAATGTT GCTGATGCTA CCCTCTCAGC CCAGGCCACA 
CCTCTGCTGC ATGCACTCAC TGCTGCCCAT GCCTACATCA TGATGTTTGT TCATACTTGC 
AGAGTTGGAC AGAGTGAAAT TCGTTCCATC TCCGTAAACC AGTGGGGCTC TCAATTGGGT 
CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA TACTGTTCCC TGGTGTGGGA AAGCACTGTC 
CTCCTCTCTC TGTGTACCCC AAACAGCCTA CCATCTGGGT GTGAATTTGG CCAGGCAGAT 
ATGCAGAAAC TGGTTCCAAA GGATGAGAAG GCAGGTACGA CCCAGGGCGG AAAAAGATCA 
GATGGGGAAC AGGATGGAGC AGCTGGAAGT ATGGATGCTT CTACCCAGGG CTTATTAGAA 
GGCATTGGGC TAGATGGTGA CACATTGGCT CCCATGGAGA CAGATGAACC TACTGCTTCA 
GACTCTAAGG GCAAATCTAA AATCACACCA GCAATGGCTG CCAGAATTAA GCAAATCAAG 
CCTTTGTTAT CAGCTTCCTC CAGATTAGGC CGAGCACTTG CTGAGCTATT TGGACTTCTT 
GTTAAACTTT GTGTGGGATC TCCTGTCCGC CAGAGAAGGA GCCATCATGC TGCCAGCACC 
ACTACAGCAC CGACACCTGC CGCGCGATCA ACAGCCTCAG CTCTCACTAA GCTCTTGACT 
AAGGGGTTAT CTTGGCAGCC CCCACCATAT ACACCTACTC CCCGATTCAG GCTGACATTC 
TTCATCTGTT CAGTTGGTTT CACATCCCCA ATGCTGTTTG ATGAGAGGAA GTATCCCTAC 
CACCTCATGC TGCAAAAATT TCTCTGCTCC GGAGGCCACA ATGCTCTTTT TGAAACTTTC 
AACTGGGCTC TGTCCATGGG AGGTAAAGTT CCTGTTTCTG AGGGATTGGA ACACTCAGAC 
TTGCCTGATG GCACAGGAGA ATTCCTAGAT GCCTGGCTTA TGCTGGTGGA GAAGATGGTG 
AATCCCACCA CGGTGCTTGA ATCTCCACAT TCGCTGCCTG CCAAATTGCC TGGAGGTGTC 
CAGAACTTTC CCCAGTTCAG TGCACTGCGC TTCCTTGTGG TAACTCAGAA AGCAGCCTTT 
ACTTGCATCA AAAACTTATG GAACCGGAAA CCCCTGAAGG TATATGGTGG ACGAATGGCT 
GAATCGATGC TGGCCATTCT ATGCCACATC CTCCGAGGAG AACCTGTGAT TCGAGAGAGA 
CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA GAAGAGGATA CAGGGCAAGA GGAAGGTGGC 
TCCCGCCGGG AACCTCAAGT CAACCAGCAA CAACTGCAAC AGCTCATGGA CATGGGCTTC 
ACAAGGGAAC ATGCAATGGA GGCACTGTTG AACACCAGCA CCATGGAGCA GGCCACAGAG 
TACCTTTTAA CCCACCCTCC TCCAATCATG GGAGGAGTTG TTCGGGATCT CAGCATGTCT 
GAAGAGGACC AGATGATGAG AGCAATTGCT ATGTCTCTGG GACAGGATAT TCCAATGGAT 
CAAAGGGCAG AGTCACCTGA GGAAGTTGCT TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT 
CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA TGTCTAGAGA AGTTCCAGGA TGCTGACCCG 
TTGGAACAAG ATGAGCTCCA CACTTTCACA GATACTATGT TGCCAGGCTG CTTCCACCTT 
CTTGATGAGC TGCCAGACAC AGTATACCGT GTGTGTGACC TGATCATGAC AGCAATCAAA 
CGTAATGGAG CAGATTATCG TGACATGATT CTGAAGCAAG TAGTCAATCA GGTGTGGGAA 
GCTGCTGATG TATTGATCAA AGCTGCTCTT CCCCTGACAA CAAGTGACAC AAAAACCGTG 
TCAGAGTGGA TAAGTCAGAT GGCCACACTG CCCCAGGCCT CCAATTTGGC TACTAGAATC 
TTGCTTTTAA CGCTACTTTT TGAGGAGTTG AAGCTACCTT GTGCTTGGGT GGTTGAATCA 
AGTGGCATCC TTAATGTCCT AATCAAACTC TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA 
GCCAAGGAGC AGAAGGAAGT CCAGACCCCA AAGTGGATCA CACCAGTGTT GCTCCTGATT 
GATTTCTATG AAAAGACAGC CATCTCCTCA AAAAGGAGAG CCCAGATGAC TAAGTACCTG 
CAATCCAACA GCAACAACTG GCGCTGGTTT GATGATCGCT CTGGGCGTTG GTGTAGTTAC 
AGTGCAAGCA ACAATAGCAC TATTGATTCT GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA 
TTCACTGCAG GCCGAAGAAG ATACACGGTC CAATTCACTA CAATGGTGCA GGTTAATGAG 
GAAACAGGGA ACCGACGCCC TGTGATGCTG ACTCTCCTCA GGGTACCTCG GCTGAATAAA 
AATTCAAAAA ACAGCAATGG ACAGGAACTA GAGAAGACGC TGGAAGAAAG CAAAGAAATG 
GATATCAAAC GTAAAGAAAA TAAAGGCAAT GATACCCCTT TGGCCCTAGA GAGTACAAAC 
ACTGAAAAGG AGACAAGCCT GGAGGAAACA AAAATCGGGG AGATCCTGAT CCAGGGCTTG 
ACAGAAGATA TGGTGACTGT TTTAATCCGG GCCTGCGTGA GCATGCTGGG AGTCCCTGTG 
GACCCAGATA CTTTGCATGC CACCCTTCGT CTCTGTCTGA GGCTCACCCG GGACCACAAA 
TATGCCATGA TGTTTGCAGA ACTGAAGAGT ACCCGCATGA TCTTGAATTT GACCCAGAGC 
TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC ACCCTTCTCT TAAGACACAT CATTGAGGAC 
CCCTGTACCC TTCGTCATAC CATGGAAAAG GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT 
GGTAGCACTA CCTCTGGTGT TGTGTCTGGC AGCCTCGGCT CTCGGGAGAT CAACTACATC 
CTTCGTGTCC TTGGGCCAGC CGCATGCCGC AATCCAGACA TATTCACAGA AGTGGCCAAC 
TGCTGTATCC GCATCGCCCT TCCTGCCCCT CGAGGCTCAG GAACTGCTTC AGATGATGAA 
TTTGAGAATC TTAGAATTAA AGGCCCTAAT GCTGTACAGC TGGTGAAGAC CACCCCTTTG 
AAGCCCTCAC CTCTGCCTGT CATCCCTGAT ACTATCAAGG AAGTGATCTA TGATATGCTG 
AATGCTCTGG CTGCATACCA TGCTCCAGAG GAAGCAGATA AATCTGATCC TAAACCTGGG 
GTTATGACCC AAGAGGTTGG CCAGCTCCTG CAAGACATGG GTGATGATGT ATACCAGCAG 
TACCGGTCAC TTACGCGTCA GAGCAGTGAC TTTGATACGC AGTCAGGTTT TTCCATTAAT 
AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC ACTGAGACTT CCGCATCTGG GACCTCCCAA 
GGAGAGGCTT CAACTCCAGA GGAGTCTCGA GATGGGAAGA AAGATAAAGA AGGGGACCGG 
GCCTCTGAGG AAGGCAAACA GAAAGGCAAG GGCAGCAAAC CTTTAATGCC TACCTCCACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCC 
AACTACAGCT ACACTGTGGG CCAGTCTGAA CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTTCTGG ACCACCTGCT CCCACATACC CAGAATGCAG AAGACAAGGA CACCCCTGCC 
TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT GCTGCAGGGA GTGGCACAGA TGCCCAGGTG 
GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT GGACGGGCAC TGGCTATGGC TGAGAGTACA 
GAGAAACATG CCAGGCTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAGTCCTGC 
CCCTCCACCT CCAGCTTCTA CAGCAGTGCC ACAGCGAAGA CCCAGCACAA TGGCATGAAC 
AACATCATTC GGCTTTTCCT GAAGAAGGGA CTGGTTAATG ACCTGGCCAG AGTACCTCAC 
AGCTTAGACC TGTCCAGTCC CAACATGGCC AACACAGTCA ATGCTGCTCT GAAGCCTTTG 
GAAACACTTT CCCGGATTGT GAACCAGCCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCCCAA GGAGCCTCTC AAGATTCCAG TAGCAACCAG 
CAGGACCCAG GCGAGCCTGG GGAAGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCTGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAGAATGA GCTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AGCACACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAGG GAGATGAAGA CGATGACGAC GATGGCTCTG AGATGGAATT GGATGAGGAT 
TATCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATCCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCATCC ACTGATGGTG CGCCATGCAG ACCACAGTTC TCTGACACTG 
GGCAGTGGCT CTTCAACAAC TCGTCTCACC CAGGGCATCG GGCGCAGTCA GAGGACCCTA 
AGGCAGCTGA CGGCCAATAC TGGCCACACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCCAACCCTC CTCTTATACT GCAGAGGTTG CTTGGTCCCT CAGCTGCTGC TGACATCCTT 
CAGCTGAGCA GCAGCCTTCC CCTACAAAGC CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC 
GATGACGTCC ACATCATCGC CCGTTCTGAT GATGAGCTGC TGGATGACTT TTTCCATGAT 
CAGAGCACAG CTACCAGCCA AGCAGGAACC CTGTCCAGCA TCCCCACAGC CCTGACCCGC 
TGGACAGAAG AATGCAAAGT TCTCGATGCT GAGAGCATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGTCCA TTGTCAATCA CCTGGAATTC CTGAGGGATG AGGAGCTGGA AGAAAGGCGA 
GAGAAGCGCA GGAAACAACT GGCTGAGGAA GAAACAAAGA TAACTGATAA AGGCAAAGAA 
GATAAGGAGA ACAGGGATCA GAGTGCACAG TGTACTGCAT CTAAGTCAAA TGACTCCACT 
GAACAGAATC TCTCAGATGG GACGCCTATG CCTGACAGCT ACCCAACAAC CCCATCTTCA 
ACTGATGCAG CTACATCTGA GTCCAAGGAG ACCCTTGGCA CTCTGCAATC CTCACAACAG 
CAACCAACAC TCCCAACCCC ACCAGCTTTG GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA 
GCTGGAGAAG GGGGCAGCTC TACACAGCTA TTGATGCCTG TAGAGCCAGA GGAATTGGGT 
CCCACAAGGC CAAGTGGGGA AGCAGAAACA ACTCAGATGG AGTTATCCCC AGCTCCCACT 
ATAACCTCAC TTTCCCCAGA GAGAGCTGAG GATTCTGATG CACTGACGGC TGTCAGCAGT 
CAGCTAGAAG GCTCTCCTAT GGATACAAGC AGCCTGGCTT CCTGTACCTT AGAGGAGGCT 
GTGGGTGACA CTTCAGCAGC TGGCAGTTCT GAGCAGCCCA GAGCAGGCAG CTCCACTCCT 
GGGGATGCCC CACCAGCTGT GGCGGAAGTG CAAGGCAGGA GTGATGGGTC AGGGGAATCT 
GCCCAGCCAC CTGAGGACAG CTCCCCACCT GCATCCTCTG AGAGCTCTTC CACCAGAGAT 
TCTGCCGTGG CCATTTCTGG AGCAGATTCC CGAGGAATCC TAGAAGAGCC GTTGCCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG GGTATCAGTC TCCCTGAAGG TGTGGACCCC 
TCTTTTCTGG CTGCCCTGCC TGATGACATC CGTCGGGAAG TTCTACAGAA CCAGCTAGGC 
ATTCGTCCAC CAACCCGGAC TGCCCCCTCC ACAAATAGCT CAGCGCCTGC AGTGGTGGGG 
AATCCTGGTG TGACTGAAGT GAGCCCTGAG TTTCTGGCTG CCCTGCCTCC AGCCATTCAG 
GAGGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029198.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884687.1 Homo sapiens HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X8, mRNA.

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CloneID	OHu99119
Clone ID Related Accession (Same CDS sequence)	XM_017029199.1 , XM_017029201.1 , XM_017029203.1 , XM_017029200.1 , XM_017029202.1 , XM_017029198.1 , XM_017029198.2
Accession Version	XM_017029198.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTTTGTT TCTCCTTTTG CAGTGTATTC CAATGCCTTG 
CAGGAATCAG CAAACAGTAT CTTGTATAAT GGCTTGATAG AGGAGTTGGT AGATGTCCTT 
CAGATAACGG ATAAGCAGCT TATGGAGATT AAAGCAGCTT CTTTACGAAC ATTAACATCA 
ATTGTCCACT TGGAGAGAAC TCCCAAACTC AGCAGTATTA TTGACTGTAC TGGAACTGCC 
TCCTACCATG GATTTTTGCC AGTGCTTGTA AGGAACTGTA TCCAGGCCAT GATTGATCCT 
TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG 
GCCAGCTACG ATGCTGGTGG TGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG 
AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCCGTC 
AGAGTGGTTG ACCTTATCAC CAACCTGGAT ATGGCAGCTT TTCAATCCCA TAGTGGACTT 
TCTATCTTCA TTTATAGACT TGAGCATGAA GTAGATTTGT GCCGAAAAGA ATGTCCGTTT 
GTGATCAAGC CAAAGATCCA GAGACCCAAT ACTACACAAG AAGGAGAGGA AATGGAAACT 
GATATGGATG TTGCAGATGT GGCTATGGAA AGCAGTCCAG GCTCATCCAT CTCTATGGAG 
CACAGGCTGG ATGTTGAATT AAGGGCATCA GGTTCCAGCA GCAGCACTAA CATCTCTTCT 
GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC GGCACCGGCC CTGGCCCCGG CCCCGGCCCC 
GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT 
GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT ATTCCACAAC GAGCAGCACT TCTGAAATCC 
ATGTTGAATT TCCTCAAGAA GGCCATCCAA GACCCTGCTT TCTCAGATGG CATACGACAT 
GTGATGGATG GTTCTCTGCC TACCTCCCTG AAACACATCA TCAGCAATGC AGAATACTAT 
GGCCCATCAC TCTTCCTCCT AGCTACTGAA GTGGTGACTG TGTTTGTATT TCAAGAACCA 
TCACTGCTCT CCTCACTCCA GGACAATGGA TTGACAGATG TCATGCTGCA TGCACTGCTT 
ATCAAAGATG TTCCTGCTAC CCGTGAAGTC CTTGGCTCCC TCCCAAATGT ATTCAGTGCA 
CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT TTTGTTCAGT GTCAGCCTTT TGAACGCCTC 
TTCAAAGTTC TTCTGTCTCC AGATTACCTC CCAGCCATGC GGAGGAGGAG AAGTTCTGAT 
CCCCTTGGGG ATACTGCATC CAACCTGGGG AGTGCTGTCG ATGAGCTCAT GAGACATCAG 
CCCACCCTTA AAACAGATGC AACGACTGCC ATCATCAAGT TACTTGAAGA AATCTGTAAT 
CTTGGAAGGG ACCCCAAATA CATCTGTCAG AAGCCATCAA TCCAGAAGGC AGATGGCACT 
GCCACTGCTC CTCCCCCAAG GTCTAATCAT GCCGCAGAAG AAGCCTCTAG TGAGGATGAG 
GAGGAAGAGG AAGTACAGGC CATGCAGAGC TTTAATTCTA CCCAGCAAAA TGAAACTGAG 
CCTAATCAGC AGGTTGTTGG TACAGAGGAA CGTATTCCTA TTCCCCTCAT GGATTACATC 
CTTAATGTGA TGAAATTTGT GGAATCTATT CTGAGCAACA ATACAACAGA TGACCACTGC 
CAGGAATTTG TGAATCAGAA AGGACTGTTG CCTTTGGTTA CCATTTTGGG TCTTCCCAAT 
CTGCCCATTG ACTTTCCCAC ATCTGCTGCC TGTCAGGCTG TTGCAGGTGT CTGCAAATCC 
ATATTGACAC TGTCACATGA ACCCAAAGTC CTTCAAGAGG GTCTCCTTCA GTTGGACTCC 
ATCCTCTCCT CCCTGGAGCC CTTACACCGC CCCATTGAAT CCCCTGGGGG CTCAGTGTTG 
TTGCGAGAAC TGGCTTGCGC AGGCAATGTT GCTGATGCTA CCCTCTCAGC CCAGGCCACA 
CCTCTGCTGC ATGCACTCAC TGCTGCCCAT GCCTACATCA TGATGTTTGT TCATACTTGC 
AGAGTTGGAC AGAGTGAAAT TCGTTCCATC TCCGTAAACC AGTGGGGCTC TCAATTGGGT 
CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA TACTGTTCCC TGGTGTGGGA AAGCACTGTC 
CTCCTCTCTC TGTGTACCCC AAACAGCCTA CCATCTGGGT GTGAATTTGG CCAGGCAGAT 
ATGCAGAAAC TGGTTCCAAA GGATGAGAAG GCAGGTACGA CCCAGGGCGG AAAAAGATCA 
GATGGGGAAC AGGATGGAGC AGCTGGAAGT ATGGATGCTT CTACCCAGGG CTTATTAGAA 
GGCATTGGGC TAGATGGTGA CACATTGGCT CCCATGGAGA CAGATGAACC TACTGCTTCA 
GACTCTAAGG GCAAATCTAA AATCACACCA GCAATGGCTG CCAGAATTAA GCAAATCAAG 
CCTTTGTTAT CAGCTTCCTC CAGATTAGGC CGAGCACTTG CTGAGCTATT TGGACTTCTT 
GTTAAACTTT GTGTGGGATC TCCTGTCCGC CAGAGAAGGA GCCATCATGC TGCCAGCACC 
ACTACAGCAC CGACACCTGC CGCGCGATCA ACAGCCTCAG CTCTCACTAA GCTCTTGACT 
AAGGGGTTAT CTTGGCAGCC CCCACCATAT ACACCTACTC CCCGATTCAG GCTGACATTC 
TTCATCTGTT CAGTTGGTTT CACATCCCCA ATGCTGTTTG ATGAGAGGAA GTATCCCTAC 
CACCTCATGC TGCAAAAATT TCTCTGCTCC GGAGGCCACA ATGCTCTTTT TGAAACTTTC 
AACTGGGCTC TGTCCATGGG AGGTAAAGTT CCTGTTTCTG AGGGATTGGA ACACTCAGAC 
TTGCCTGATG GCACAGGAGA ATTCCTAGAT GCCTGGCTTA TGCTGGTGGA GAAGATGGTG 
AATCCCACCA CGGTGCTTGA ATCTCCACAT TCGCTGCCTG CCAAATTGCC TGGAGGTGTC 
CAGAACTTTC CCCAGTTCAG TGCACTGCGC TTCCTTGTGG TAACTCAGAA AGCAGCCTTT 
ACTTGCATCA AAAACTTATG GAACCGGAAA CCCCTGAAGG TATATGGTGG ACGAATGGCT 
GAATCGATGC TGGCCATTCT ATGCCACATC CTCCGAGGAG AACCTGTGAT TCGAGAGAGA 
CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA GAAGAGGATA CAGGGCAAGA GGAAGGTGGC 
TCCCGCCGGG AACCTCAAGT CAACCAGCAA CAACTGCAAC AGCTCATGGA CATGGGCTTC 
ACAAGGGAAC ATGCAATGGA GGCACTGTTG AACACCAGCA CCATGGAGCA GGCCACAGAG 
TACCTTTTAA CCCACCCTCC TCCAATCATG GGAGGAGTTG TTCGGGATCT CAGCATGTCT 
GAAGAGGACC AGATGATGAG AGCAATTGCT ATGTCTCTGG GACAGGATAT TCCAATGGAT 
CAAAGGGCAG AGTCACCTGA GGAAGTTGCT TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT 
CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA TGTCTAGAGA AGTTCCAGGA TGCTGACCCG 
TTGGAACAAG ATGAGCTCCA CACTTTCACA GATACTATGT TGCCAGGCTG CTTCCACCTT 
CTTGATGAGC TGCCAGACAC AGTATACCGT GTGTGTGACC TGATCATGAC AGCAATCAAA 
CGTAATGGAG CAGATTATCG TGACATGATT CTGAAGCAAG TAGTCAATCA GGTGTGGGAA 
GCTGCTGATG TATTGATCAA AGCTGCTCTT CCCCTGACAA CAAGTGACAC AAAAACCGTG 
TCAGAGTGGA TAAGTCAGAT GGCCACACTG CCCCAGGCCT CCAATTTGGC TACTAGAATC 
TTGCTTTTAA CGCTACTTTT TGAGGAGTTG AAGCTACCTT GTGCTTGGGT GGTTGAATCA 
AGTGGCATCC TTAATGTCCT AATCAAACTC TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA 
GCCAAGGAGC AGAAGGAAGT CCAGACCCCA AAGTGGATCA CACCAGTGTT GCTCCTGATT 
GATTTCTATG AAAAGACAGC CATCTCCTCA AAAAGGAGAG CCCAGATGAC TAAGTACCTG 
CAATCCAACA GCAACAACTG GCGCTGGTTT GATGATCGCT CTGGGCGTTG GTGTAGTTAC 
AGTGCAAGCA ACAATAGCAC TATTGATTCT GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA 
TTCACTGCAG GCCGAAGAAG ATACACGGTC CAATTCACTA CAATGGTGCA GGTTAATGAG 
GAAACAGGGA ACCGACGCCC TGTGATGCTG ACTCTCCTCA GGGTACCTCG GCTGAATAAA 
AATTCAAAAA ACAGCAATGG ACAGGAACTA GAGAAGACGC TGGAAGAAAG CAAAGAAATG 
GATATCAAAC GTAAAGAAAA TAAAGGCAAT GATACCCCTT TGGCCCTAGA GAGTACAAAC 
ACTGAAAAGG AGACAAGCCT GGAGGAAACA AAAATCGGGG AGATCCTGAT CCAGGGCTTG 
ACAGAAGATA TGGTGACTGT TTTAATCCGG GCCTGCGTGA GCATGCTGGG AGTCCCTGTG 
GACCCAGATA CTTTGCATGC CACCCTTCGT CTCTGTCTGA GGCTCACCCG GGACCACAAA 
TATGCCATGA TGTTTGCAGA ACTGAAGAGT ACCCGCATGA TCTTGAATTT GACCCAGAGC 
TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC ACCCTTCTCT TAAGACACAT CATTGAGGAC 
CCCTGTACCC TTCGTCATAC CATGGAAAAG GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT 
GGTAGCACTA CCTCTGGTGT TGTGTCTGGC AGCCTCGGCT CTCGGGAGAT CAACTACATC 
CTTCGTGTCC TTGGGCCAGC CGCATGCCGC AATCCAGACA TATTCACAGA AGTGGCCAAC 
TGCTGTATCC GCATCGCCCT TCCTGCCCCT CGAGGCTCAG GAACTGCTTC AGATGATGAA 
TTTGAGAATC TTAGAATTAA AGGCCCTAAT GCTGTACAGC TGGTGAAGAC CACCCCTTTG 
AAGCCCTCAC CTCTGCCTGT CATCCCTGAT ACTATCAAGG AAGTGATCTA TGATATGCTG 
AATGCTCTGG CTGCATACCA TGCTCCAGAG GAAGCAGATA AATCTGATCC TAAACCTGGG 
GTTATGACCC AAGAGGTTGG CCAGCTCCTG CAAGACATGG GTGATGATGT ATACCAGCAG 
TACCGGTCAC TTACGCGTCA GAGCAGTGAC TTTGATACGC AGTCAGGTTT TTCCATTAAT 
AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC ACTGAGACTT CCGCATCTGG GACCTCCCAA 
GGAGAGGCTT CAACTCCAGA GGAGTCTCGA GATGGGAAGA AAGATAAAGA AGGGGACCGG 
GCCTCTGAGG AAGGCAAACA GAAAGGCAAG GGCAGCAAAC CTTTAATGCC TACCTCCACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCC 
AACTACAGCT ACACTGTGGG CCAGTCTGAA CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTTCTGG ACCACCTGCT CCCACATACC CAGAATGCAG AAGACAAGGA CACCCCTGCC 
TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT GCTGCAGGGA GTGGCACAGA TGCCCAGGTG 
GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT GGACGGGCAC TGGCTATGGC TGAGAGTACA 
GAGAAACATG CCAGGCTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAGTCCTGC 
CCCTCCACCT CCAGCTTCTA CAGCAGTGCC ACAGCGAAGA CCCAGCACAA TGGCATGAAC 
AACATCATTC GGCTTTTCCT GAAGAAGGGA CTGGTTAATG ACCTGGCCAG AGTACCTCAC 
AGCTTAGACC TGTCCAGTCC CAACATGGCC AACACAGTCA ATGCTGCTCT GAAGCCTTTG 
GAAACACTTT CCCGGATTGT GAACCAGCCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCCCAA GGAGCCTCTC AAGATTCCAG TAGCAACCAG 
CAGGACCCAG GCGAGCCTGG GGAAGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCTGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAGAATGA GCTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AGCACACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAGG GAGATGAAGA CGATGACGAC GATGGCTCTG AGATGGAATT GGATGAGGAT 
TATCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATCCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCATCC ACTGATGGTG CGCCATGCAG ACCACAGTTC TCTGACACTG 
GGCAGTGGCT CTTCAACAAC TCGTCTCACC CAGGGCATCG GGCGCAGTCA GAGGACCCTA 
AGGCAGCTGA CGGCCAATAC TGGCCACACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCCAACCCTC CTCTTATACT GCAGAGGTTG CTTGGTCCCT CAGCTGCTGC TGACATCCTT 
CAGCTGAGCA GCAGCCTTCC CCTACAAAGC CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC 
GATGACGTCC ACATCATCGC CCGTTCTGAT GATGAGCTGC TGGATGACTT TTTCCATGAT 
CAGAGCACAG CTACCAGCCA AGCAGGAACC CTGTCCAGCA TCCCCACAGC CCTGACCCGC 
TGGACAGAAG AATGCAAAGT TCTCGATGCT GAGAGCATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGTCCA TTGTCAATCA CCTGGAATTC CTGAGGGATG AGGAGCTGGA AGAAAGGCGA 
GAGAAGCGCA GGAAACAACT GGCTGAGGAA GAAACAAAGA TAACTGATAA AGGCAAAGAA 
GATAAGGAGA ACAGGGATCA GAGTGCACAG TGTACTGCAT CTAAGTCAAA TGACTCCACT 
GAACAGAATC TCTCAGATGG GACGCCTATG CCTGACAGCT ACCCAACAAC CCCATCTTCA 
ACTGATGCAG CTACATCTGA GTCCAAGGAG ACCCTTGGCA CTCTGCAATC CTCACAACAG 
CAACCAACAC TCCCAACCCC ACCAGCTTTG GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA 
GCTGGAGAAG GGGGCAGCTC TACACAGCTA TTGATGCCTG TAGAGCCAGA GGAATTGGGT 
CCCACAAGGC CAAGTGGGGA AGCAGAAACA ACTCAGATGG AGTTATCCCC AGCTCCCACT 
ATAACCTCAC TTTCCCCAGA GAGAGCTGAG GATTCTGATG CACTGACGGC TGTCAGCAGT 
CAGCTAGAAG GCTCTCCTAT GGATACAAGC AGCCTGGCTT CCTGTACCTT AGAGGAGGCT 
GTGGGTGACA CTTCAGCAGC TGGCAGTTCT GAGCAGCCCA GAGCAGGCAG CTCCACTCCT 
GGGGATGCCC CACCAGCTGT GGCGGAAGTG CAAGGCAGGA GTGATGGGTC AGGGGAATCT 
GCCCAGCCAC CTGAGGACAG CTCCCCACCT GCATCCTCTG AGAGCTCTTC CACCAGAGAT 
TCTGCCGTGG CCATTTCTGG AGCAGATTCC CGAGGAATCC TAGAAGAGCC GTTGCCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG GGTATCAGTC TCCCTGAAGG TGTGGACCCC 
TCTTTTCTGG CTGCCCTGCC TGATGACATC CGTCGGGAAG TTCTACAGAA CCAGCTAGGC 
ATTCGTCCAC CAACCCGGAC TGCCCCCTCC ACAAATAGCT CAGCGCCTGC AGTGGTGGGG 
AATCCTGGTG TGACTGAAGT GAGCCCTGAG TTTCTGGCTG CCCTGCCTCC AGCCATTCAG 
GAGGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884687.1
CDS	471..13865
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029198.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029198.1

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTTTGTT TCTCCTTTTG CAGTGTATTC CAATGCCTTG 
CAGGAATCAG CAAACAGTAT CTTGTATAAT GGCTTGATAG AGGAGTTGGT AGATGTCCTT 
CAGATAACGG ATAAGCAGCT TATGGAGATT AAAGCAGCTT CTTTACGAAC ATTAACATCA 
ATTGTCCACT TGGAGAGAAC TCCCAAACTC AGCAGTATTA TTGACTGTAC TGGAACTGCC 
TCCTACCATG GATTTTTGCC AGTGCTTGTA AGGAACTGTA TCCAGGCCAT GATTGATCCT 
TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG 
GCCAGCTACG ATGCTGGTGG TGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG 
AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCCGTC 
AGAGTGGTTG ACCTTATCAC CAACCTGGAT ATGGCAGCTT TTCAATCCCA TAGTGGACTT 
TCTATCTTCA TTTATAGACT TGAGCATGAA GTAGATTTGT GCCGAAAAGA ATGTCCGTTT 
GTGATCAAGC CAAAGATCCA GAGACCCAAT ACTACACAAG AAGGAGAGGA AATGGAAACT 
GATATGGATG TTGCAGATGT GGCTATGGAA AGCAGTCCAG GCTCATCCAT CTCTATGGAG 
CACAGGCTGG ATGTTGAATT AAGGGCATCA GGTTCCAGCA GCAGCACTAA CATCTCTTCT 
GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC GGCACCGGCC CTGGCCCCGG CCCCGGCCCC 
GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT 
GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT ATTCCACAAC GAGCAGCACT TCTGAAATCC 
ATGTTGAATT TCCTCAAGAA GGCCATCCAA GACCCTGCTT TCTCAGATGG CATACGACAT 
GTGATGGATG GTTCTCTGCC TACCTCCCTG AAACACATCA TCAGCAATGC AGAATACTAT 
GGCCCATCAC TCTTCCTCCT AGCTACTGAA GTGGTGACTG TGTTTGTATT TCAAGAACCA 
TCACTGCTCT CCTCACTCCA GGACAATGGA TTGACAGATG TCATGCTGCA TGCACTGCTT 
ATCAAAGATG TTCCTGCTAC CCGTGAAGTC CTTGGCTCCC TCCCAAATGT ATTCAGTGCA 
CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT TTTGTTCAGT GTCAGCCTTT TGAACGCCTC 
TTCAAAGTTC TTCTGTCTCC AGATTACCTC CCAGCCATGC GGAGGAGGAG AAGTTCTGAT 
CCCCTTGGGG ATACTGCATC CAACCTGGGG AGTGCTGTCG ATGAGCTCAT GAGACATCAG 
CCCACCCTTA AAACAGATGC AACGACTGCC ATCATCAAGT TACTTGAAGA AATCTGTAAT 
CTTGGAAGGG ACCCCAAATA CATCTGTCAG AAGCCATCAA TCCAGAAGGC AGATGGCACT 
GCCACTGCTC CTCCCCCAAG GTCTAATCAT GCCGCAGAAG AAGCCTCTAG TGAGGATGAG 
GAGGAAGAGG AAGTACAGGC CATGCAGAGC TTTAATTCTA CCCAGCAAAA TGAAACTGAG 
CCTAATCAGC AGGTTGTTGG TACAGAGGAA CGTATTCCTA TTCCCCTCAT GGATTACATC 
CTTAATGTGA TGAAATTTGT GGAATCTATT CTGAGCAACA ATACAACAGA TGACCACTGC 
CAGGAATTTG TGAATCAGAA AGGACTGTTG CCTTTGGTTA CCATTTTGGG TCTTCCCAAT 
CTGCCCATTG ACTTTCCCAC ATCTGCTGCC TGTCAGGCTG TTGCAGGTGT CTGCAAATCC 
ATATTGACAC TGTCACATGA ACCCAAAGTC CTTCAAGAGG GTCTCCTTCA GTTGGACTCC 
ATCCTCTCCT CCCTGGAGCC CTTACACCGC CCCATTGAAT CCCCTGGGGG CTCAGTGTTG 
TTGCGAGAAC TGGCTTGCGC AGGCAATGTT GCTGATGCTA CCCTCTCAGC CCAGGCCACA 
CCTCTGCTGC ATGCACTCAC TGCTGCCCAT GCCTACATCA TGATGTTTGT TCATACTTGC 
AGAGTTGGAC AGAGTGAAAT TCGTTCCATC TCCGTAAACC AGTGGGGCTC TCAATTGGGT 
CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA TACTGTTCCC TGGTGTGGGA AAGCACTGTC 
CTCCTCTCTC TGTGTACCCC AAACAGCCTA CCATCTGGGT GTGAATTTGG CCAGGCAGAT 
ATGCAGAAAC TGGTTCCAAA GGATGAGAAG GCAGGTACGA CCCAGGGCGG AAAAAGATCA 
GATGGGGAAC AGGATGGAGC AGCTGGAAGT ATGGATGCTT CTACCCAGGG CTTATTAGAA 
GGCATTGGGC TAGATGGTGA CACATTGGCT CCCATGGAGA CAGATGAACC TACTGCTTCA 
GACTCTAAGG GCAAATCTAA AATCACACCA GCAATGGCTG CCAGAATTAA GCAAATCAAG 
CCTTTGTTAT CAGCTTCCTC CAGATTAGGC CGAGCACTTG CTGAGCTATT TGGACTTCTT 
GTTAAACTTT GTGTGGGATC TCCTGTCCGC CAGAGAAGGA GCCATCATGC TGCCAGCACC 
ACTACAGCAC CGACACCTGC CGCGCGATCA ACAGCCTCAG CTCTCACTAA GCTCTTGACT 
AAGGGGTTAT CTTGGCAGCC CCCACCATAT ACACCTACTC CCCGATTCAG GCTGACATTC 
TTCATCTGTT CAGTTGGTTT CACATCCCCA ATGCTGTTTG ATGAGAGGAA GTATCCCTAC 
CACCTCATGC TGCAAAAATT TCTCTGCTCC GGAGGCCACA ATGCTCTTTT TGAAACTTTC 
AACTGGGCTC TGTCCATGGG AGGTAAAGTT CCTGTTTCTG AGGGATTGGA ACACTCAGAC 
TTGCCTGATG GCACAGGAGA ATTCCTAGAT GCCTGGCTTA TGCTGGTGGA GAAGATGGTG 
AATCCCACCA CGGTGCTTGA ATCTCCACAT TCGCTGCCTG CCAAATTGCC TGGAGGTGTC 
CAGAACTTTC CCCAGTTCAG TGCACTGCGC TTCCTTGTGG TAACTCAGAA AGCAGCCTTT 
ACTTGCATCA AAAACTTATG GAACCGGAAA CCCCTGAAGG TATATGGTGG ACGAATGGCT 
GAATCGATGC TGGCCATTCT ATGCCACATC CTCCGAGGAG AACCTGTGAT TCGAGAGAGA 
CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA GAAGAGGATA CAGGGCAAGA GGAAGGTGGC 
TCCCGCCGGG AACCTCAAGT CAACCAGCAA CAACTGCAAC AGCTCATGGA CATGGGCTTC 
ACAAGGGAAC ATGCAATGGA GGCACTGTTG AACACCAGCA CCATGGAGCA GGCCACAGAG 
TACCTTTTAA CCCACCCTCC TCCAATCATG GGAGGAGTTG TTCGGGATCT CAGCATGTCT 
GAAGAGGACC AGATGATGAG AGCAATTGCT ATGTCTCTGG GACAGGATAT TCCAATGGAT 
CAAAGGGCAG AGTCACCTGA GGAAGTTGCT TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT 
CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA TGTCTAGAGA AGTTCCAGGA TGCTGACCCG 
TTGGAACAAG ATGAGCTCCA CACTTTCACA GATACTATGT TGCCAGGCTG CTTCCACCTT 
CTTGATGAGC TGCCAGACAC AGTATACCGT GTGTGTGACC TGATCATGAC AGCAATCAAA 
CGTAATGGAG CAGATTATCG TGACATGATT CTGAAGCAAG TAGTCAATCA GGTGTGGGAA 
GCTGCTGATG TATTGATCAA AGCTGCTCTT CCCCTGACAA CAAGTGACAC AAAAACCGTG 
TCAGAGTGGA TAAGTCAGAT GGCCACACTG CCCCAGGCCT CCAATTTGGC TACTAGAATC 
TTGCTTTTAA CGCTACTTTT TGAGGAGTTG AAGCTACCTT GTGCTTGGGT GGTTGAATCA 
AGTGGCATCC TTAATGTCCT AATCAAACTC TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA 
GCCAAGGAGC AGAAGGAAGT CCAGACCCCA AAGTGGATCA CACCAGTGTT GCTCCTGATT 
GATTTCTATG AAAAGACAGC CATCTCCTCA AAAAGGAGAG CCCAGATGAC TAAGTACCTG 
CAATCCAACA GCAACAACTG GCGCTGGTTT GATGATCGCT CTGGGCGTTG GTGTAGTTAC 
AGTGCAAGCA ACAATAGCAC TATTGATTCT GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA 
TTCACTGCAG GCCGAAGAAG ATACACGGTC CAATTCACTA CAATGGTGCA GGTTAATGAG 
GAAACAGGGA ACCGACGCCC TGTGATGCTG ACTCTCCTCA GGGTACCTCG GCTGAATAAA 
AATTCAAAAA ACAGCAATGG ACAGGAACTA GAGAAGACGC TGGAAGAAAG CAAAGAAATG 
GATATCAAAC GTAAAGAAAA TAAAGGCAAT GATACCCCTT TGGCCCTAGA GAGTACAAAC 
ACTGAAAAGG AGACAAGCCT GGAGGAAACA AAAATCGGGG AGATCCTGAT CCAGGGCTTG 
ACAGAAGATA TGGTGACTGT TTTAATCCGG GCCTGCGTGA GCATGCTGGG AGTCCCTGTG 
GACCCAGATA CTTTGCATGC CACCCTTCGT CTCTGTCTGA GGCTCACCCG GGACCACAAA 
TATGCCATGA TGTTTGCAGA ACTGAAGAGT ACCCGCATGA TCTTGAATTT GACCCAGAGC 
TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC ACCCTTCTCT TAAGACACAT CATTGAGGAC 
CCCTGTACCC TTCGTCATAC CATGGAAAAG GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT 
GGTAGCACTA CCTCTGGTGT TGTGTCTGGC AGCCTCGGCT CTCGGGAGAT CAACTACATC 
CTTCGTGTCC TTGGGCCAGC CGCATGCCGC AATCCAGACA TATTCACAGA AGTGGCCAAC 
TGCTGTATCC GCATCGCCCT TCCTGCCCCT CGAGGCTCAG GAACTGCTTC AGATGATGAA 
TTTGAGAATC TTAGAATTAA AGGCCCTAAT GCTGTACAGC TGGTGAAGAC CACCCCTTTG 
AAGCCCTCAC CTCTGCCTGT CATCCCTGAT ACTATCAAGG AAGTGATCTA TGATATGCTG 
AATGCTCTGG CTGCATACCA TGCTCCAGAG GAAGCAGATA AATCTGATCC TAAACCTGGG 
GTTATGACCC AAGAGGTTGG CCAGCTCCTG CAAGACATGG GTGATGATGT ATACCAGCAG 
TACCGGTCAC TTACGCGTCA GAGCAGTGAC TTTGATACGC AGTCAGGTTT TTCCATTAAT 
AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC ACTGAGACTT CCGCATCTGG GACCTCCCAA 
GGAGAGGCTT CAACTCCAGA GGAGTCTCGA GATGGGAAGA AAGATAAAGA AGGGGACCGG 
GCCTCTGAGG AAGGCAAACA GAAAGGCAAG GGCAGCAAAC CTTTAATGCC TACCTCCACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCC 
AACTACAGCT ACACTGTGGG CCAGTCTGAA CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTTCTGG ACCACCTGCT CCCACATACC CAGAATGCAG AAGACAAGGA CACCCCTGCC 
TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT GCTGCAGGGA GTGGCACAGA TGCCCAGGTG 
GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT GGACGGGCAC TGGCTATGGC TGAGAGTACA 
GAGAAACATG CCAGGCTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAGTCCTGC 
CCCTCCACCT CCAGCTTCTA CAGCAGTGCC ACAGCGAAGA CCCAGCACAA TGGCATGAAC 
AACATCATTC GGCTTTTCCT GAAGAAGGGA CTGGTTAATG ACCTGGCCAG AGTACCTCAC 
AGCTTAGACC TGTCCAGTCC CAACATGGCC AACACAGTCA ATGCTGCTCT GAAGCCTTTG 
GAAACACTTT CCCGGATTGT GAACCAGCCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCCCAA GGAGCCTCTC AAGATTCCAG TAGCAACCAG 
CAGGACCCAG GCGAGCCTGG GGAAGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCTGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAGAATGA GCTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AGCACACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAGG GAGATGAAGA CGATGACGAC GATGGCTCTG AGATGGAATT GGATGAGGAT 
TATCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATCCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCATCC ACTGATGGTG CGCCATGCAG ACCACAGTTC TCTGACACTG 
GGCAGTGGCT CTTCAACAAC TCGTCTCACC CAGGGCATCG GGCGCAGTCA GAGGACCCTA 
AGGCAGCTGA CGGCCAATAC TGGCCACACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCCAACCCTC CTCTTATACT GCAGAGGTTG CTTGGTCCCT CAGCTGCTGC TGACATCCTT 
CAGCTGAGCA GCAGCCTTCC CCTACAAAGC CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC 
GATGACGTCC ACATCATCGC CCGTTCTGAT GATGAGCTGC TGGATGACTT TTTCCATGAT 
CAGAGCACAG CTACCAGCCA AGCAGGAACC CTGTCCAGCA TCCCCACAGC CCTGACCCGC 
TGGACAGAAG AATGCAAAGT TCTCGATGCT GAGAGCATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGTCCA TTGTCAATCA CCTGGAATTC CTGAGGGATG AGGAGCTGGA AGAAAGGCGA 
GAGAAGCGCA GGAAACAACT GGCTGAGGAA GAAACAAAGA TAACTGATAA AGGCAAAGAA 
GATAAGGAGA ACAGGGATCA GAGTGCACAG TGTACTGCAT CTAAGTCAAA TGACTCCACT 
GAACAGAATC TCTCAGATGG GACGCCTATG CCTGACAGCT ACCCAACAAC CCCATCTTCA 
ACTGATGCAG CTACATCTGA GTCCAAGGAG ACCCTTGGCA CTCTGCAATC CTCACAACAG 
CAACCAACAC TCCCAACCCC ACCAGCTTTG GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA 
GCTGGAGAAG GGGGCAGCTC TACACAGCTA TTGATGCCTG TAGAGCCAGA GGAATTGGGT 
CCCACAAGGC CAAGTGGGGA AGCAGAAACA ACTCAGATGG AGTTATCCCC AGCTCCCACT 
ATAACCTCAC TTTCCCCAGA GAGAGCTGAG GATTCTGATG CACTGACGGC TGTCAGCAGT 
CAGCTAGAAG GCTCTCCTAT GGATACAAGC AGCCTGGCTT CCTGTACCTT AGAGGAGGCT 
GTGGGTGACA CTTCAGCAGC TGGCAGTTCT GAGCAGCCCA GAGCAGGCAG CTCCACTCCT 
GGGGATGCCC CACCAGCTGT GGCGGAAGTG CAAGGCAGGA GTGATGGGTC AGGGGAATCT 
GCCCAGCCAC CTGAGGACAG CTCCCCACCT GCATCCTCTG AGAGCTCTTC CACCAGAGAT 
TCTGCCGTGG CCATTTCTGG AGCAGATTCC CGAGGAATCC TAGAAGAGCC GTTGCCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG GGTATCAGTC TCCCTGAAGG TGTGGACCCC 
TCTTTTCTGG CTGCCCTGCC TGATGACATC CGTCGGGAAG TTCTACAGAA CCAGCTAGGC 
ATTCGTCCAC CAACCCGGAC TGCCCCCTCC ACAAATAGCT CAGCGCCTGC AGTGGTGGGG 
AATCCTGGTG TGACTGAAGT GAGCCCTGAG TTTCTGGCTG CCCTGCCTCC AGCCATTCAG 
GAGGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029198.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884687.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X8, mRNA.

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CloneID	OHu99119
Clone ID Related Accession (Same CDS sequence)	XM_017029199.1 , XM_017029201.1 , XM_017029203.1 , XM_017029200.1 , XM_017029202.1 , XM_017029198.1 , XM_017029198.2
Accession Version	XM_017029198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTTTGTT TCTCCTTTTG CAGTGTATTC CAATGCCTTG 
CAGGAATCAG CAAACAGTAT CTTGTATAAT GGCTTGATAG AGGAGTTGGT AGATGTCCTT 
CAGATAACGG ATAAGCAGCT TATGGAGATT AAAGCAGCTT CTTTACGAAC ATTAACATCA 
ATTGTCCACT TGGAGAGAAC TCCCAAACTC AGCAGTATTA TTGACTGTAC TGGAACTGCC 
TCCTACCATG GATTTTTGCC AGTGCTTGTA AGGAACTGTA TCCAGGCCAT GATTGATCCT 
TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG 
GCCAGCTACG ATGCTGGTGG TGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG 
AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCCGTC 
AGAGTGGTTG ACCTTATCAC CAACCTGGAT ATGGCAGCTT TTCAATCCCA TAGTGGACTT 
TCTATCTTCA TTTATAGACT TGAGCATGAA GTAGATTTGT GCCGAAAAGA ATGTCCGTTT 
GTGATCAAGC CAAAGATCCA GAGACCCAAT ACTACACAAG AAGGAGAGGA AATGGAAACT 
GATATGGATG TTGCAGATGT GGCTATGGAA AGCAGTCCAG GCTCATCCAT CTCTATGGAG 
CACAGGCTGG ATGTTGAATT AAGGGCATCA GGTTCCAGCA GCAGCACTAA CATCTCTTCT 
GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC GGCACCGGCC CTGGCCCCGG CCCCGGCCCC 
GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT 
GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT ATTCCACAAC GAGCAGCACT TCTGAAATCC 
ATGTTGAATT TCCTCAAGAA GGCCATCCAA GACCCTGCTT TCTCAGATGG CATACGACAT 
GTGATGGATG GTTCTCTGCC TACCTCCCTG AAACACATCA TCAGCAATGC AGAATACTAT 
GGCCCATCAC TCTTCCTCCT AGCTACTGAA GTGGTGACTG TGTTTGTATT TCAAGAACCA 
TCACTGCTCT CCTCACTCCA GGACAATGGA TTGACAGATG TCATGCTGCA TGCACTGCTT 
ATCAAAGATG TTCCTGCTAC CCGTGAAGTC CTTGGCTCCC TCCCAAATGT ATTCAGTGCA 
CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT TTTGTTCAGT GTCAGCCTTT TGAACGCCTC 
TTCAAAGTTC TTCTGTCTCC AGATTACCTC CCAGCCATGC GGAGGAGGAG AAGTTCTGAT 
CCCCTTGGGG ATACTGCATC CAACCTGGGG AGTGCTGTCG ATGAGCTCAT GAGACATCAG 
CCCACCCTTA AAACAGATGC AACGACTGCC ATCATCAAGT TACTTGAAGA AATCTGTAAT 
CTTGGAAGGG ACCCCAAATA CATCTGTCAG AAGCCATCAA TCCAGAAGGC AGATGGCACT 
GCCACTGCTC CTCCCCCAAG GTCTAATCAT GCCGCAGAAG AAGCCTCTAG TGAGGATGAG 
GAGGAAGAGG AAGTACAGGC CATGCAGAGC TTTAATTCTA CCCAGCAAAA TGAAACTGAG 
CCTAATCAGC AGGTTGTTGG TACAGAGGAA CGTATTCCTA TTCCCCTCAT GGATTACATC 
CTTAATGTGA TGAAATTTGT GGAATCTATT CTGAGCAACA ATACAACAGA TGACCACTGC 
CAGGAATTTG TGAATCAGAA AGGACTGTTG CCTTTGGTTA CCATTTTGGG TCTTCCCAAT 
CTGCCCATTG ACTTTCCCAC ATCTGCTGCC TGTCAGGCTG TTGCAGGTGT CTGCAAATCC 
ATATTGACAC TGTCACATGA ACCCAAAGTC CTTCAAGAGG GTCTCCTTCA GTTGGACTCC 
ATCCTCTCCT CCCTGGAGCC CTTACACCGC CCCATTGAAT CCCCTGGGGG CTCAGTGTTG 
TTGCGAGAAC TGGCTTGCGC AGGCAATGTT GCTGATGCTA CCCTCTCAGC CCAGGCCACA 
CCTCTGCTGC ATGCACTCAC TGCTGCCCAT GCCTACATCA TGATGTTTGT TCATACTTGC 
AGAGTTGGAC AGAGTGAAAT TCGTTCCATC TCCGTAAACC AGTGGGGCTC TCAATTGGGT 
CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA TACTGTTCCC TGGTGTGGGA AAGCACTGTC 
CTCCTCTCTC TGTGTACCCC AAACAGCCTA CCATCTGGGT GTGAATTTGG CCAGGCAGAT 
ATGCAGAAAC TGGTTCCAAA GGATGAGAAG GCAGGTACGA CCCAGGGCGG AAAAAGATCA 
GATGGGGAAC AGGATGGAGC AGCTGGAAGT ATGGATGCTT CTACCCAGGG CTTATTAGAA 
GGCATTGGGC TAGATGGTGA CACATTGGCT CCCATGGAGA CAGATGAACC TACTGCTTCA 
GACTCTAAGG GCAAATCTAA AATCACACCA GCAATGGCTG CCAGAATTAA GCAAATCAAG 
CCTTTGTTAT CAGCTTCCTC CAGATTAGGC CGAGCACTTG CTGAGCTATT TGGACTTCTT 
GTTAAACTTT GTGTGGGATC TCCTGTCCGC CAGAGAAGGA GCCATCATGC TGCCAGCACC 
ACTACAGCAC CGACACCTGC CGCGCGATCA ACAGCCTCAG CTCTCACTAA GCTCTTGACT 
AAGGGGTTAT CTTGGCAGCC CCCACCATAT ACACCTACTC CCCGATTCAG GCTGACATTC 
TTCATCTGTT CAGTTGGTTT CACATCCCCA ATGCTGTTTG ATGAGAGGAA GTATCCCTAC 
CACCTCATGC TGCAAAAATT TCTCTGCTCC GGAGGCCACA ATGCTCTTTT TGAAACTTTC 
AACTGGGCTC TGTCCATGGG AGGTAAAGTT CCTGTTTCTG AGGGATTGGA ACACTCAGAC 
TTGCCTGATG GCACAGGAGA ATTCCTAGAT GCCTGGCTTA TGCTGGTGGA GAAGATGGTG 
AATCCCACCA CGGTGCTTGA ATCTCCACAT TCGCTGCCTG CCAAATTGCC TGGAGGTGTC 
CAGAACTTTC CCCAGTTCAG TGCACTGCGC TTCCTTGTGG TAACTCAGAA AGCAGCCTTT 
ACTTGCATCA AAAACTTATG GAACCGGAAA CCCCTGAAGG TATATGGTGG ACGAATGGCT 
GAATCGATGC TGGCCATTCT ATGCCACATC CTCCGAGGAG AACCTGTGAT TCGAGAGAGA 
CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA GAAGAGGATA CAGGGCAAGA GGAAGGTGGC 
TCCCGCCGGG AACCTCAAGT CAACCAGCAA CAACTGCAAC AGCTCATGGA CATGGGCTTC 
ACAAGGGAAC ATGCAATGGA GGCACTGTTG AACACCAGCA CCATGGAGCA GGCCACAGAG 
TACCTTTTAA CCCACCCTCC TCCAATCATG GGAGGAGTTG TTCGGGATCT CAGCATGTCT 
GAAGAGGACC AGATGATGAG AGCAATTGCT ATGTCTCTGG GACAGGATAT TCCAATGGAT 
CAAAGGGCAG AGTCACCTGA GGAAGTTGCT TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT 
CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA TGTCTAGAGA AGTTCCAGGA TGCTGACCCG 
TTGGAACAAG ATGAGCTCCA CACTTTCACA GATACTATGT TGCCAGGCTG CTTCCACCTT 
CTTGATGAGC TGCCAGACAC AGTATACCGT GTGTGTGACC TGATCATGAC AGCAATCAAA 
CGTAATGGAG CAGATTATCG TGACATGATT CTGAAGCAAG TAGTCAATCA GGTGTGGGAA 
GCTGCTGATG TATTGATCAA AGCTGCTCTT CCCCTGACAA CAAGTGACAC AAAAACCGTG 
TCAGAGTGGA TAAGTCAGAT GGCCACACTG CCCCAGGCCT CCAATTTGGC TACTAGAATC 
TTGCTTTTAA CGCTACTTTT TGAGGAGTTG AAGCTACCTT GTGCTTGGGT GGTTGAATCA 
AGTGGCATCC TTAATGTCCT AATCAAACTC TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA 
GCCAAGGAGC AGAAGGAAGT CCAGACCCCA AAGTGGATCA CACCAGTGTT GCTCCTGATT 
GATTTCTATG AAAAGACAGC CATCTCCTCA AAAAGGAGAG CCCAGATGAC TAAGTACCTG 
CAATCCAACA GCAACAACTG GCGCTGGTTT GATGATCGCT CTGGGCGTTG GTGTAGTTAC 
AGTGCAAGCA ACAATAGCAC TATTGATTCT GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA 
TTCACTGCAG GCCGAAGAAG ATACACGGTC CAATTCACTA CAATGGTGCA GGTTAATGAG 
GAAACAGGGA ACCGACGCCC TGTGATGCTG ACTCTCCTCA GGGTACCTCG GCTGAATAAA 
AATTCAAAAA ACAGCAATGG ACAGGAACTA GAGAAGACGC TGGAAGAAAG CAAAGAAATG 
GATATCAAAC GTAAAGAAAA TAAAGGCAAT GATACCCCTT TGGCCCTAGA GAGTACAAAC 
ACTGAAAAGG AGACAAGCCT GGAGGAAACA AAAATCGGGG AGATCCTGAT CCAGGGCTTG 
ACAGAAGATA TGGTGACTGT TTTAATCCGG GCCTGCGTGA GCATGCTGGG AGTCCCTGTG 
GACCCAGATA CTTTGCATGC CACCCTTCGT CTCTGTCTGA GGCTCACCCG GGACCACAAA 
TATGCCATGA TGTTTGCAGA ACTGAAGAGT ACCCGCATGA TCTTGAATTT GACCCAGAGC 
TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC ACCCTTCTCT TAAGACACAT CATTGAGGAC 
CCCTGTACCC TTCGTCATAC CATGGAAAAG GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT 
GGTAGCACTA CCTCTGGTGT TGTGTCTGGC AGCCTCGGCT CTCGGGAGAT CAACTACATC 
CTTCGTGTCC TTGGGCCAGC CGCATGCCGC AATCCAGACA TATTCACAGA AGTGGCCAAC 
TGCTGTATCC GCATCGCCCT TCCTGCCCCT CGAGGCTCAG GAACTGCTTC AGATGATGAA 
TTTGAGAATC TTAGAATTAA AGGCCCTAAT GCTGTACAGC TGGTGAAGAC CACCCCTTTG 
AAGCCCTCAC CTCTGCCTGT CATCCCTGAT ACTATCAAGG AAGTGATCTA TGATATGCTG 
AATGCTCTGG CTGCATACCA TGCTCCAGAG GAAGCAGATA AATCTGATCC TAAACCTGGG 
GTTATGACCC AAGAGGTTGG CCAGCTCCTG CAAGACATGG GTGATGATGT ATACCAGCAG 
TACCGGTCAC TTACGCGTCA GAGCAGTGAC TTTGATACGC AGTCAGGTTT TTCCATTAAT 
AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC ACTGAGACTT CCGCATCTGG GACCTCCCAA 
GGAGAGGCTT CAACTCCAGA GGAGTCTCGA GATGGGAAGA AAGATAAAGA AGGGGACCGG 
GCCTCTGAGG AAGGCAAACA GAAAGGCAAG GGCAGCAAAC CTTTAATGCC TACCTCCACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCC 
AACTACAGCT ACACTGTGGG CCAGTCTGAA CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTTCTGG ACCACCTGCT CCCACATACC CAGAATGCAG AAGACAAGGA CACCCCTGCC 
TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT GCTGCAGGGA GTGGCACAGA TGCCCAGGTG 
GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT GGACGGGCAC TGGCTATGGC TGAGAGTACA 
GAGAAACATG CCAGGCTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAGTCCTGC 
CCCTCCACCT CCAGCTTCTA CAGCAGTGCC ACAGCGAAGA CCCAGCACAA TGGCATGAAC 
AACATCATTC GGCTTTTCCT GAAGAAGGGA CTGGTTAATG ACCTGGCCAG AGTACCTCAC 
AGCTTAGACC TGTCCAGTCC CAACATGGCC AACACAGTCA ATGCTGCTCT GAAGCCTTTG 
GAAACACTTT CCCGGATTGT GAACCAGCCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCCCAA GGAGCCTCTC AAGATTCCAG TAGCAACCAG 
CAGGACCCAG GCGAGCCTGG GGAAGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCTGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAGAATGA GCTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AGCACACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAGG GAGATGAAGA CGATGACGAC GATGGCTCTG AGATGGAATT GGATGAGGAT 
TATCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATCCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCATCC ACTGATGGTG CGCCATGCAG ACCACAGTTC TCTGACACTG 
GGCAGTGGCT CTTCAACAAC TCGTCTCACC CAGGGCATCG GGCGCAGTCA GAGGACCCTA 
AGGCAGCTGA CGGCCAATAC TGGCCACACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCCAACCCTC CTCTTATACT GCAGAGGTTG CTTGGTCCCT CAGCTGCTGC TGACATCCTT 
CAGCTGAGCA GCAGCCTTCC CCTACAAAGC CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC 
GATGACGTCC ACATCATCGC CCGTTCTGAT GATGAGCTGC TGGATGACTT TTTCCATGAT 
CAGAGCACAG CTACCAGCCA AGCAGGAACC CTGTCCAGCA TCCCCACAGC CCTGACCCGC 
TGGACAGAAG AATGCAAAGT TCTCGATGCT GAGAGCATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGTCCA TTGTCAATCA CCTGGAATTC CTGAGGGATG AGGAGCTGGA AGAAAGGCGA 
GAGAAGCGCA GGAAACAACT GGCTGAGGAA GAAACAAAGA TAACTGATAA AGGCAAAGAA 
GATAAGGAGA ACAGGGATCA GAGTGCACAG TGTACTGCAT CTAAGTCAAA TGACTCCACT 
GAACAGAATC TCTCAGATGG GACGCCTATG CCTGACAGCT ACCCAACAAC CCCATCTTCA 
ACTGATGCAG CTACATCTGA GTCCAAGGAG ACCCTTGGCA CTCTGCAATC CTCACAACAG 
CAACCAACAC TCCCAACCCC ACCAGCTTTG GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA 
GCTGGAGAAG GGGGCAGCTC TACACAGCTA TTGATGCCTG TAGAGCCAGA GGAATTGGGT 
CCCACAAGGC CAAGTGGGGA AGCAGAAACA ACTCAGATGG AGTTATCCCC AGCTCCCACT 
ATAACCTCAC TTTCCCCAGA GAGAGCTGAG GATTCTGATG CACTGACGGC TGTCAGCAGT 
CAGCTAGAAG GCTCTCCTAT GGATACAAGC AGCCTGGCTT CCTGTACCTT AGAGGAGGCT 
GTGGGTGACA CTTCAGCAGC TGGCAGTTCT GAGCAGCCCA GAGCAGGCAG CTCCACTCCT 
GGGGATGCCC CACCAGCTGT GGCGGAAGTG CAAGGCAGGA GTGATGGGTC AGGGGAATCT 
GCCCAGCCAC CTGAGGACAG CTCCCCACCT GCATCCTCTG AGAGCTCTTC CACCAGAGAT 
TCTGCCGTGG CCATTTCTGG AGCAGATTCC CGAGGAATCC TAGAAGAGCC GTTGCCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG GGTATCAGTC TCCCTGAAGG TGTGGACCCC 
TCTTTTCTGG CTGCCCTGCC TGATGACATC CGTCGGGAAG TTCTACAGAA CCAGCTAGGC 
ATTCGTCCAC CAACCCGGAC TGCCCCCTCC ACAAATAGCT CAGCGCCTGC AGTGGTGGGG 
AATCCTGGTG TGACTGAAGT GAGCCCTGAG TTTCTGGCTG CCCTGCCTCC AGCCATTCAG 
GAGGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884687.1
CDS	433..13827
Translation	MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029198.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029198.2

ORF Insert Sequence:

ATGAAAGTAG ACAGGACTAA ACTGAAGAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TTCTCTTGGA ACTGCAGCAG 
ATCAAAACAT GGAACATTGG AAAGTGCGAG TTATATCACT GGGTGGACCT GTTGGACCGC 
TTCGATGGAA TACTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTCGTA 
TGTGATAGGC CAGAAAGAGA GCAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA 
GCCTTGCTCA TTGAGTACAG CTTTTCCCGG CATCTGTACA GTTCCATAGA GCATTTGACA 
ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTCAATCT CCTATATGTA 
TTTAGCAAAA GATCAAACTA CATCACTCGT CTGGGATCTG ACAAGAGGAC CCCGCTGCTA 
ACTCGGCTAC AACATTTGGC AGAGAGCTGG GGTGGAAAGG AGAATGGCTT TGGACTTGCA 
GAATGTTGCA GAGACTTGCA TATGATGAAA TATCCACCCA GTGCAACTAC ACTACACTTT 
GAATTCTATG CAGATCCTGG GGCCGAGGTC AAAATTGAGA AAAGGACAAC TAGTAACACA 
CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG 
GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACACAC 
ATACGACTGG CCCATGGCTT TTCTAATCAC AGGAAGCGAT TGCAGGCAGT TCAGGCCAGA 
CTGCATGCAA TATCTATATT AGTGTTTGTT TCTCCTTTTG CAGTGTATTC CAATGCCTTG 
CAGGAATCAG CAAACAGTAT CTTGTATAAT GGCTTGATAG AGGAGTTGGT AGATGTCCTT 
CAGATAACGG ATAAGCAGCT TATGGAGATT AAAGCAGCTT CTTTACGAAC ATTAACATCA 
ATTGTCCACT TGGAGAGAAC TCCCAAACTC AGCAGTATTA TTGACTGTAC TGGAACTGCC 
TCCTACCATG GATTTTTGCC AGTGCTTGTA AGGAACTGTA TCCAGGCCAT GATTGATCCT 
TCCATGGATC CATACCCTCA CCAGTTTGCC ACTGCTCTCT TCTCTTTTTT ATACCATCTG 
GCCAGCTACG ATGCTGGTGG TGAAGCCTTG GTCTCCTGTG GAATGATGGA AGCCTTATTG 
AAGGTCATAA AGTTTCTTGG CGATGAACAG GACCAGATAA CATTTGTCAC CAGAGCCGTC 
AGAGTGGTTG ACCTTATCAC CAACCTGGAT ATGGCAGCTT TTCAATCCCA TAGTGGACTT 
TCTATCTTCA TTTATAGACT TGAGCATGAA GTAGATTTGT GCCGAAAAGA ATGTCCGTTT 
GTGATCAAGC CAAAGATCCA GAGACCCAAT ACTACACAAG AAGGAGAGGA AATGGAAACT 
GATATGGATG TTGCAGATGT GGCTATGGAA AGCAGTCCAG GCTCATCCAT CTCTATGGAG 
CACAGGCTGG ATGTTGAATT AAGGGCATCA GGTTCCAGCA GCAGCACTAA CATCTCTTCT 
GGCCCCAGCC CTGGTCCCAG TCCCGGCCCC GGCACCGGCC CTGGCCCCGG CCCCGGCCCC 
GGCCCCGGCC CTGGCCCCGG CCCCGGCCCC GGTCCTGGTC CCGGCCCTGG CCCCGGCCCT 
GGCCCTGGCC CCCGTCCTGG AGTCCAGTGT ATTCCACAAC GAGCAGCACT TCTGAAATCC 
ATGTTGAATT TCCTCAAGAA GGCCATCCAA GACCCTGCTT TCTCAGATGG CATACGACAT 
GTGATGGATG GTTCTCTGCC TACCTCCCTG AAACACATCA TCAGCAATGC AGAATACTAT 
GGCCCATCAC TCTTCCTCCT AGCTACTGAA GTGGTGACTG TGTTTGTATT TCAAGAACCA 
TCACTGCTCT CCTCACTCCA GGACAATGGA TTGACAGATG TCATGCTGCA TGCACTGCTT 
ATCAAAGATG TTCCTGCTAC CCGTGAAGTC CTTGGCTCCC TCCCAAATGT ATTCAGTGCA 
CTCTGTTTGA ATGCCCGAGG TCTTCAGTCT TTTGTTCAGT GTCAGCCTTT TGAACGCCTC 
TTCAAAGTTC TTCTGTCTCC AGATTACCTC CCAGCCATGC GGAGGAGGAG AAGTTCTGAT 
CCCCTTGGGG ATACTGCATC CAACCTGGGG AGTGCTGTCG ATGAGCTCAT GAGACATCAG 
CCCACCCTTA AAACAGATGC AACGACTGCC ATCATCAAGT TACTTGAAGA AATCTGTAAT 
CTTGGAAGGG ACCCCAAATA CATCTGTCAG AAGCCATCAA TCCAGAAGGC AGATGGCACT 
GCCACTGCTC CTCCCCCAAG GTCTAATCAT GCCGCAGAAG AAGCCTCTAG TGAGGATGAG 
GAGGAAGAGG AAGTACAGGC CATGCAGAGC TTTAATTCTA CCCAGCAAAA TGAAACTGAG 
CCTAATCAGC AGGTTGTTGG TACAGAGGAA CGTATTCCTA TTCCCCTCAT GGATTACATC 
CTTAATGTGA TGAAATTTGT GGAATCTATT CTGAGCAACA ATACAACAGA TGACCACTGC 
CAGGAATTTG TGAATCAGAA AGGACTGTTG CCTTTGGTTA CCATTTTGGG TCTTCCCAAT 
CTGCCCATTG ACTTTCCCAC ATCTGCTGCC TGTCAGGCTG TTGCAGGTGT CTGCAAATCC 
ATATTGACAC TGTCACATGA ACCCAAAGTC CTTCAAGAGG GTCTCCTTCA GTTGGACTCC 
ATCCTCTCCT CCCTGGAGCC CTTACACCGC CCCATTGAAT CCCCTGGGGG CTCAGTGTTG 
TTGCGAGAAC TGGCTTGCGC AGGCAATGTT GCTGATGCTA CCCTCTCAGC CCAGGCCACA 
CCTCTGCTGC ATGCACTCAC TGCTGCCCAT GCCTACATCA TGATGTTTGT TCATACTTGC 
AGAGTTGGAC AGAGTGAAAT TCGTTCCATC TCCGTAAACC AGTGGGGCTC TCAATTGGGT 
CTGAGTGTTT TGAGCAAGCT GAGCCAGTTA TACTGTTCCC TGGTGTGGGA AAGCACTGTC 
CTCCTCTCTC TGTGTACCCC AAACAGCCTA CCATCTGGGT GTGAATTTGG CCAGGCAGAT 
ATGCAGAAAC TGGTTCCAAA GGATGAGAAG GCAGGTACGA CCCAGGGCGG AAAAAGATCA 
GATGGGGAAC AGGATGGAGC AGCTGGAAGT ATGGATGCTT CTACCCAGGG CTTATTAGAA 
GGCATTGGGC TAGATGGTGA CACATTGGCT CCCATGGAGA CAGATGAACC TACTGCTTCA 
GACTCTAAGG GCAAATCTAA AATCACACCA GCAATGGCTG CCAGAATTAA GCAAATCAAG 
CCTTTGTTAT CAGCTTCCTC CAGATTAGGC CGAGCACTTG CTGAGCTATT TGGACTTCTT 
GTTAAACTTT GTGTGGGATC TCCTGTCCGC CAGAGAAGGA GCCATCATGC TGCCAGCACC 
ACTACAGCAC CGACACCTGC CGCGCGATCA ACAGCCTCAG CTCTCACTAA GCTCTTGACT 
AAGGGGTTAT CTTGGCAGCC CCCACCATAT ACACCTACTC CCCGATTCAG GCTGACATTC 
TTCATCTGTT CAGTTGGTTT CACATCCCCA ATGCTGTTTG ATGAGAGGAA GTATCCCTAC 
CACCTCATGC TGCAAAAATT TCTCTGCTCC GGAGGCCACA ATGCTCTTTT TGAAACTTTC 
AACTGGGCTC TGTCCATGGG AGGTAAAGTT CCTGTTTCTG AGGGATTGGA ACACTCAGAC 
TTGCCTGATG GCACAGGAGA ATTCCTAGAT GCCTGGCTTA TGCTGGTGGA GAAGATGGTG 
AATCCCACCA CGGTGCTTGA ATCTCCACAT TCGCTGCCTG CCAAATTGCC TGGAGGTGTC 
CAGAACTTTC CCCAGTTCAG TGCACTGCGC TTCCTTGTGG TAACTCAGAA AGCAGCCTTT 
ACTTGCATCA AAAACTTATG GAACCGGAAA CCCCTGAAGG TATATGGTGG ACGAATGGCT 
GAATCGATGC TGGCCATTCT ATGCCACATC CTCCGAGGAG AACCTGTGAT TCGAGAGAGA 
CTAAGCAAGG AGAAGGAGGG GTCTCGAGGA GAAGAGGATA CAGGGCAAGA GGAAGGTGGC 
TCCCGCCGGG AACCTCAAGT CAACCAGCAA CAACTGCAAC AGCTCATGGA CATGGGCTTC 
ACAAGGGAAC ATGCAATGGA GGCACTGTTG AACACCAGCA CCATGGAGCA GGCCACAGAG 
TACCTTTTAA CCCACCCTCC TCCAATCATG GGAGGAGTTG TTCGGGATCT CAGCATGTCT 
GAAGAGGACC AGATGATGAG AGCAATTGCT ATGTCTCTGG GACAGGATAT TCCAATGGAT 
CAAAGGGCAG AGTCACCTGA GGAAGTTGCT TGCCGGAAGG AGGAAGAGGA ACGGAAAGCT 
CGGGAAAAGC AGGAGGAGGA AGAGGCTAAA TGTCTAGAGA AGTTCCAGGA TGCTGACCCG 
TTGGAACAAG ATGAGCTCCA CACTTTCACA GATACTATGT TGCCAGGCTG CTTCCACCTT 
CTTGATGAGC TGCCAGACAC AGTATACCGT GTGTGTGACC TGATCATGAC AGCAATCAAA 
CGTAATGGAG CAGATTATCG TGACATGATT CTGAAGCAAG TAGTCAATCA GGTGTGGGAA 
GCTGCTGATG TATTGATCAA AGCTGCTCTT CCCCTGACAA CAAGTGACAC AAAAACCGTG 
TCAGAGTGGA TAAGTCAGAT GGCCACACTG CCCCAGGCCT CCAATTTGGC TACTAGAATC 
TTGCTTTTAA CGCTACTTTT TGAGGAGTTG AAGCTACCTT GTGCTTGGGT GGTTGAATCA 
AGTGGCATCC TTAATGTCCT AATCAAACTC TTGGAAGTGG TTCAGCCCTG CCTCCAGGCA 
GCCAAGGAGC AGAAGGAAGT CCAGACCCCA AAGTGGATCA CACCAGTGTT GCTCCTGATT 
GATTTCTATG AAAAGACAGC CATCTCCTCA AAAAGGAGAG CCCAGATGAC TAAGTACCTG 
CAATCCAACA GCAACAACTG GCGCTGGTTT GATGATCGCT CTGGGCGTTG GTGTAGTTAC 
AGTGCAAGCA ACAATAGCAC TATTGATTCT GCCTGGAAAT CTGGAGAGAC AAGCGTGCGA 
TTCACTGCAG GCCGAAGAAG ATACACGGTC CAATTCACTA CAATGGTGCA GGTTAATGAG 
GAAACAGGGA ACCGACGCCC TGTGATGCTG ACTCTCCTCA GGGTACCTCG GCTGAATAAA 
AATTCAAAAA ACAGCAATGG ACAGGAACTA GAGAAGACGC TGGAAGAAAG CAAAGAAATG 
GATATCAAAC GTAAAGAAAA TAAAGGCAAT GATACCCCTT TGGCCCTAGA GAGTACAAAC 
ACTGAAAAGG AGACAAGCCT GGAGGAAACA AAAATCGGGG AGATCCTGAT CCAGGGCTTG 
ACAGAAGATA TGGTGACTGT TTTAATCCGG GCCTGCGTGA GCATGCTGGG AGTCCCTGTG 
GACCCAGATA CTTTGCATGC CACCCTTCGT CTCTGTCTGA GGCTCACCCG GGACCACAAA 
TATGCCATGA TGTTTGCAGA ACTGAAGAGT ACCCGCATGA TCTTGAATTT GACCCAGAGC 
TCAGGCTTCA ATGGGTTTAC TCCCCTGGTC ACCCTTCTCT TAAGACACAT CATTGAGGAC 
CCCTGTACCC TTCGTCATAC CATGGAAAAG GTTGTTCGCT CAGCAGCTAC AAGTGGAGCT 
GGTAGCACTA CCTCTGGTGT TGTGTCTGGC AGCCTCGGCT CTCGGGAGAT CAACTACATC 
CTTCGTGTCC TTGGGCCAGC CGCATGCCGC AATCCAGACA TATTCACAGA AGTGGCCAAC 
TGCTGTATCC GCATCGCCCT TCCTGCCCCT CGAGGCTCAG GAACTGCTTC AGATGATGAA 
TTTGAGAATC TTAGAATTAA AGGCCCTAAT GCTGTACAGC TGGTGAAGAC CACCCCTTTG 
AAGCCCTCAC CTCTGCCTGT CATCCCTGAT ACTATCAAGG AAGTGATCTA TGATATGCTG 
AATGCTCTGG CTGCATACCA TGCTCCAGAG GAAGCAGATA AATCTGATCC TAAACCTGGG 
GTTATGACCC AAGAGGTTGG CCAGCTCCTG CAAGACATGG GTGATGATGT ATACCAGCAG 
TACCGGTCAC TTACGCGTCA GAGCAGTGAC TTTGATACGC AGTCAGGTTT TTCCATTAAT 
AGTCAGGTCT TTGCTGCAGA TGGTGCCTCC ACTGAGACTT CCGCATCTGG GACCTCCCAA 
GGAGAGGCTT CAACTCCAGA GGAGTCTCGA GATGGGAAGA AAGATAAAGA AGGGGACCGG 
GCCTCTGAGG AAGGCAAACA GAAAGGCAAG GGCAGCAAAC CTTTAATGCC TACCTCCACT 
ATCCTTCGTC TTCTGGCAGA GTTGGTGAGG TCCTATGTTG GTATTGCTAC CCTGATTGCC 
AACTACAGCT ACACTGTGGG CCAGTCTGAA CTGATCAAAG AGGACTGCAG TGTGCTAGCT 
TTTGTTCTGG ACCACCTGCT CCCACATACC CAGAATGCAG AAGACAAGGA CACCCCTGCC 
TTGGCCCGCC TGTTCCTCGC AAGCCTGGCT GCTGCAGGGA GTGGCACAGA TGCCCAGGTG 
GCCCTAGTGA ATGAAGTAAA AGCAGCCCTT GGACGGGCAC TGGCTATGGC TGAGAGTACA 
GAGAAACATG CCAGGCTTCA GGCAGTGATG TGTATCATCA GTACTATCAT GGAGTCCTGC 
CCCTCCACCT CCAGCTTCTA CAGCAGTGCC ACAGCGAAGA CCCAGCACAA TGGCATGAAC 
AACATCATTC GGCTTTTCCT GAAGAAGGGA CTGGTTAATG ACCTGGCCAG AGTACCTCAC 
AGCTTAGACC TGTCCAGTCC CAACATGGCC AACACAGTCA ATGCTGCTCT GAAGCCTTTG 
GAAACACTTT CCCGGATTGT GAACCAGCCC AGTAGCCTTT TTGGCAGCAA GAGTGCTTCT 
AGCAAGAACA AGTCTGAGCA GGATGCCCAA GGAGCCTCTC AAGATTCCAG TAGCAACCAG 
CAGGACCCAG GCGAGCCTGG GGAAGCAGAA GTGCAGGAGG AGGATCATGA TGTCACTCAG 
ACAGAGGTGG CAGATGGGGA TATCATGGAT GGGGAGGCTG AAACCGACTC AGTGGTGATT 
GCTGGGCAGC CTGAGGTGCT CAGTTCACAA GAGATGCAGG TTGAGAATGA GCTGGAGGAC 
CTGATAGATG AGTTGCTTGA GAGGGATGGC GGATCTGGGA ACAGTACAAT TATAGTGAGC 
AGAAGTGGAG AGGATGAATC ACAAGAGGAC GTGCTGATGG ATGAAGCTCC TTCCAACCTC 
AGCCAAGCTT CCACCTTGCA GGCCAACCGA GAAGATTCCA TGAATATCCT GGACCCTGAG 
GATGAGGAGG AGCACACTCA GGAAGAGGAC AGCAGTGGCA GTAACGAGGA TGAGGATGAT 
AGTCAGGATG AAGAGGAGGA GGAGGAGGAA GATGAGGAAG ATGATCAGGA GGATGATGAA 
GGTGAAGAGG GAGATGAAGA CGATGACGAC GATGGCTCTG AGATGGAATT GGATGAGGAT 
TATCCTGATA TGAACGCTTC TCCCTTGGTC CGATTTGAGC GCTTTGACCG GGAGGATGAT 
CTCATCATTG AGTTTGACAA CATGTTCTCC AGTGCTACAG ACATCCCCCC ATCCCCAGGA 
AATATCCCTA CCACCCATCC ACTGATGGTG CGCCATGCAG ACCACAGTTC TCTGACACTG 
GGCAGTGGCT CTTCAACAAC TCGTCTCACC CAGGGCATCG GGCGCAGTCA GAGGACCCTA 
AGGCAGCTGA CGGCCAATAC TGGCCACACC ATTCATGTTC ACTACCCTGG GAATCGCCAG 
CCCAACCCTC CTCTTATACT GCAGAGGTTG CTTGGTCCCT CAGCTGCTGC TGACATCCTT 
CAGCTGAGCA GCAGCCTTCC CCTACAAAGC CGGGGTCGGG CCCGCCTCCT GGTAGGCAAC 
GATGACGTCC ACATCATCGC CCGTTCTGAT GATGAGCTGC TGGATGACTT TTTCCATGAT 
CAGAGCACAG CTACCAGCCA AGCAGGAACC CTGTCCAGCA TCCCCACAGC CCTGACCCGC 
TGGACAGAAG AATGCAAAGT TCTCGATGCT GAGAGCATGC ATGACTGTGT TTCAGTGGTT 
AAAGTGTCCA TTGTCAATCA CCTGGAATTC CTGAGGGATG AGGAGCTGGA AGAAAGGCGA 
GAGAAGCGCA GGAAACAACT GGCTGAGGAA GAAACAAAGA TAACTGATAA AGGCAAAGAA 
GATAAGGAGA ACAGGGATCA GAGTGCACAG TGTACTGCAT CTAAGTCAAA TGACTCCACT 
GAACAGAATC TCTCAGATGG GACGCCTATG CCTGACAGCT ACCCAACAAC CCCATCTTCA 
ACTGATGCAG CTACATCTGA GTCCAAGGAG ACCCTTGGCA CTCTGCAATC CTCACAACAG 
CAACCAACAC TCCCAACCCC ACCAGCTTTG GGAGAGGTTC CTCAGGAGCT GCAGTCTCCA 
GCTGGAGAAG GGGGCAGCTC TACACAGCTA TTGATGCCTG TAGAGCCAGA GGAATTGGGT 
CCCACAAGGC CAAGTGGGGA AGCAGAAACA ACTCAGATGG AGTTATCCCC AGCTCCCACT 
ATAACCTCAC TTTCCCCAGA GAGAGCTGAG GATTCTGATG CACTGACGGC TGTCAGCAGT 
CAGCTAGAAG GCTCTCCTAT GGATACAAGC AGCCTGGCTT CCTGTACCTT AGAGGAGGCT 
GTGGGTGACA CTTCAGCAGC TGGCAGTTCT GAGCAGCCCA GAGCAGGCAG CTCCACTCCT 
GGGGATGCCC CACCAGCTGT GGCGGAAGTG CAAGGCAGGA GTGATGGGTC AGGGGAATCT 
GCCCAGCCAC CTGAGGACAG CTCCCCACCT GCATCCTCTG AGAGCTCTTC CACCAGAGAT 
TCTGCCGTGG CCATTTCTGG AGCAGATTCC CGAGGAATCC TAGAAGAGCC GTTGCCTTCA 
ACAAGCAGTG AAGAAGAAGA TCCCCTTGCG GGTATCAGTC TCCCTGAAGG TGTGGACCCC 
TCTTTTCTGG CTGCCCTGCC TGATGACATC CGTCGGGAAG TTCTACAGAA CCAGCTAGGC 
ATTCGTCCAC CAACCCGGAC TGCCCCCTCC ACAAATAGCT CAGCGCCTGC AGTGGTGGGG 
AATCCTGGTG TGACTGAAGT GAGCCCTGAG TTTCTGGCTG CCCTGCCTCC AGCCATTCAG 
GAGGAAGTAC TGGCACAGCA GAGAGCTGAG CAGCAGCGAC GAGAACTAGC ACAGAATGCC 
AGCTCAGACA CCCCTATGGA CCCTGTGACC TTCATCCAGA CTCTGCCCTC AGACCTGCGC 
CGTAGTGTCC TAGAGGATAT GGAGGACAGT GTGTTAGCTG TGATGCCACC TGACATTGCA 
GCTGAGGCTC AAGCCCTGAG ACGAGAGCAA GAAGCCCGGC AGCGACAGCT CATGCATGAG 
CGTCTGTTTG GGCACAGTAG CACCTCCGCA CTCTCTGCTA TTCTCCGAAG CCCGGCTTTC 
ACCAGTCGCT TAAGTGGCAA CCGTGGGGTC CAGTATACTC GCCTTGCTGT GCAGAGAGGT 
GGCACCTTCC AGATGGGGGG TAGCAGCAGC CATAACAGGC CTTCTGGCAG TAATGTAGAT 
ACTCTCCTCC GCCTCCGAGG ACGGCTCCTT CTGGACCACG AAGCCCTTTC TTGTCTCTTG 
GTCCTACTTT TTGTGGATGA GCCAAAGCTC AATACTAGCC GTCTACACCG AGTACTGAGA 
AATCTCTGCT ACCATGCCCA GACCCGCCAC TGGGTCATCC GCAGTCTGCT CTCCATCTTG 
CAGCGCAGCA GTGAGAGTGA GCTATGCATT GAAACACCCA AACTCACTAC AAGTGAGGAA 
AAGGGCAAAA AGTCGAGCAA GAGCTGTGGG TCAAGTAGCC ATGAGAACCG TCCCCTGGAC 
CTGCTACACA AGATGGAGTC AAAGAGCTCC AACCAGCTTT CCTGGCTCTC AGTATCCATG 
GATGCAGCCC TAGGCTGCAG GACTAATATA TTTCAGATCC AGCGTTCAGG GGGGCGTAAA 
CATACCGAGA AGCATGCAAG CGGTGGCTCC ACCGTCCACA TCCATCCCCA AGCTGCTCCT 
GTTGTCTGCA GACACGTTTT GGATACACTC ATTCAATTGG CCAAGGTATT TCCCAGCCAC 
TTCACACAGC AGCGGACCAA AGAAACAAAC TGTGAGAGTG ATCGGGAAAG GGGCAATAAG 
GCCTGTAGCC CATGCTCCTC ACAGTCCTCC AGCAGTGGCA TTTGCACAGA CTTCTGGGAC 
TTATTGGTAA AACTGGACAA CATGAATGTC AGCCGGAAAG GCAAGAACTC CGTGAAGTCA 
GTGCCAGTGA GCGCTGGCGG TGAGGGGGAA ACCTCTCCAT ACAGCCTCGA GGCCTCTCCA 
CTGGGGCAGC TCATGAACAT GTTGTCACAC CCAGTCATCC GCCGGAGCTC TCTCTTAACT 
GAGAAACTCC TCAGACTCCT TTCTCTCATC TCAATTGCTC TCCCAGAAAA CAAGGTGTCA 
GAAGCACAGG CTAATTCTGG CAGCGGTGCT TCCTCCACCA CCACTGCCAC CTCAACCACA 
TCTACCACCA CCACCACTGC CGCCTCCACC ACGCCCACAC CCCCTACTGC ACCCACCCCT 
GTCACTTCTG CTCCAGCCCT GGTTGCTGCC ACGGCTATTT CCACCATTGT CGTAGCTGCT 
TCGACCACAG TGACTACCCC CACGACTGCT ACCACTACTG TTTCAATTTC TCCCACTACT 
AAGGGCAGCA AATCTCCAGC GAAGGTGAGT GATGGGGGCA GCAGCAGTAC AGACTTTAAG 
ATGGTGTCCT CTGGCCTCAC TGAAAACCAG CTACAGCTCT CTGTAGAGGT GTTGACATCC 
CACTCTTGTT CTGAGGAAGG CTTAGAGGAT GCAGCCAACG TACTACTGCA GCTCTCCCGG 
GGGGACTCTG GGACCCGGGA CACTGTTCTC AAGCTGCTAC TGAATGGAGC CCGCCATCTG 
GGTTATACCC TTTGTAAACA AATAGGTACC CTGCTGGCCG AGCTGCGGGA ATACAACCTC 
GAGCAGCAGC GGCGAGCCCA ATGTGAAACC CTCTCTCCTG ATGGCCTGCC TGAGGAGCAG 
CCACAGACCA CCAAGCTGAA GGGCAAAATG CAGAGCAGGT TTGACATGGC TGAGAATGTG 
GTAATTGTGG CATCTCAGAA GCGACCTTTG GGTGGCCGGG AGCTCCAGCT GCCTTCTATG 
TCCATGTTGA CATCCAAGAC ATCTACCCAG AAGTTCTTCT TGAGGGTACT ACAGGTCATC 
ATCCAGCTCC GGGACGACAC GCGCCGGGCT AACAAGAAAG CCAAGCAGAC AGGCAGGCTA 
GGTTCCTCCG GTTTAGGCTC AGCTAGCAGC ATCCAGGCAG CTGTTCGGCA GCTGGAGGCT 
GAGGCTGATG CCATTATACA AATGGTACGT GAGGGTCAAA GGGCGCGGAG ACAGCAACAA 
GCAGCAACGT CGGAGTCTAG CCAGTCAGAG GCGTCTGTCC GGAGGGAGGA ATCACCCATG 
GATGTGGACC AGCCATCTCC CAGTGCTCAA GATACTCAAT CCATTGCCTC CGATGGAACC 
CCACAGGGGG AGAAGGAAAA GGAAGAAAGA CCACCTGAGT TACCCCTGCT CAGCGAGCAG 
CTGAGTTTGG ACGAGCTGTG GGACATGCTT GGGGAGTGTC TAAAGGAACT AGAGGAATCC 
CATGACCAGC ATGCGGTGCT AGTGCTACAG CCTGCTGTCG AGGCCTTCTT TCTGGTCCAT 
GCCACAGAGC GGGAGAGCAA GCCTCCTGTC CGAGACACCC GTGAGAGCCA GCTGGCACAC 
ATCAAGGACG AGCCTCCTCC ACTCTCCCCT GCCCCCTTAA CCCCAGCCAC GCCTTCCTCC 
CTTGACCCAT TCTTCTCCCG GGAGCCCTCA TCTATGCACA TCTCCTCAAG CCTGCCCCCT 
GACACACAGA AGTTCCTTCG CTTTGCAGAG ACTCACCGCA CTGTGTTAAA CCAGATCCTA 
CGGCAGTCCA CGACCCACCT TGCTGATGGG CCTTTTGCTG TCCTGGTAGA CTACATTCGT 
GTCCTCGACT TTGATGTCAA GCGCAAATAT TTCCGCCAAG AGCTGGAGCG TTTAGATGAG 
GGGCTCCGGA AAGAAGACAT GGCTGTGCAT GTCCGTCGTG ACCATGTGTT TGAAGACTCC 
TATCGTGAGC TGCATCGCAA ATCCCCCGAA GAAATGAAGA ATCGATTGTA TATAGTATTT 
GAAGGAGAAG AAGGGCAGGA TGCTGGTGGG CTCCTGCGGG AGTGGTATAT GATCATCTCT 
CGAGAGATGT TTAACCCTAT GTATGCCTTG TTCCGTACCT CACCTGGTGA TCGAGTCACC 
TACACCATCA ATCCATCTTC CCACTGCAAC CCCAACCACC TCAGCTACTT CAAGTTTGTC 
GGACGCATTG TGGCCAAAGC TGTATATGAC AACCGTCTTC TGGAGTGCTA CTTTACTCGA 
TCCTTTTACA AACACATCTT GGGCAAGTCA GTCAGATATA CAGATATGGA GAGTGAAGAT 
TACCACTTCT ACCAAGGTCT GGTTTATCTG CTGGAAAATG ATGTCTCCAC ACTAGGCTAT 
GACCTCACCT TCAGCACTGA GGTCCAAGAG TTTGGAGTTT GTGAAGTTCG TGACCTCAAA 
CCCAATGGGG CCAACATCTT GGTAACAGAG GAGAATAAGA AGGAGTATGT ACACCTGGTA 
TGCCAGATGA GAATGACAGG AGCCATCCGC AAGCAGTTGG CGGCTTTCTT AGAAGGCTTC 
TATGAGATCA TTCCAAAGCG CCTCATTTCC ATCTTCACTG AGCAGGAGTT AGAGCTGCTT 
ATATCAGGAC TGCCCACCAT TGACATCGAT GATCTGAAAT CCAACACTGA ATACCACAAG 
TACCAGTCCA ACTCTATTCA GATCCAGTGG TTCTGGAGAG CATTGCGTTC TTTCGATCAA 
GCTGACCGTG CCAAGTTCCT CCAGTTTGTC ACAGGTACTT CCAAGGTACC CCTGCAAGGC 
TTTGCTGCCC TCGAAGGCAT GAATGGCATT CAGAAGTTTC AGATCCATCG AGATGACAGG 
TCCACAGATC GCCTGCCTTC AGCTCACACA TGTTTTAATC AGCTGGATCT GCCTGCCTAT 
GAGAGCTTTG AGAAGCTCCG CCACATGCTA CTGTTGGCTA TCCAGGAGTG CTCTGAAGGC 
TTTGGGCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029204.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884693.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X17, mRNA.

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CloneID	OHu99120
Clone ID Related Accession (Same CDS sequence)	XM_017029204.1
Accession Version	XM_017029204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GGAGTCCAGT GTATTCCACA ACGAGCAGCA CTTCTGAAAT CCATGTTGAA TTTCCTCAAG 
AAGGCCATCC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTCTG 
CCTACCTCCC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGCCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAAC CATCACTGCT CTCCTCACTC 
CAGGACAATG GATTGACAGA TGTCATGCTG CATGCACTGC TTATCAAAGA TGTTCCTGCT 
ACCCGTGAAG TCCTTGGCTC CCTCCCAAAT GTATTCAGTG CACTCTGTTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTTCA GTGTCAGCCT TTTGAACGCC TCTTCAAAGT TCTTCTGTCT 
CCAGATTACC TCCCAGCCAT GCGGAGGAGG AGAAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT CGATGAGCTC ATGAGACATC AGCCCACCCT TAAAACAGAT 
GCAACGACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG GGACCCCAAA 
TACATCTGTC AGAAGCCATC AATCCAGAAG GCAGATGGCA CTGCCACTGC TCCTCCCCCA 
AGGTCTAATC ATGCCGCAGA AGAAGCCTCT AGTGAGGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TACCCAGCAA AATGAAACTG AGCCTAATCA GCAGGTTGTT 
GGTACAGAGG AACGTATTCC TATTCCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTCTGAGCAA CAATACAACA GATGACCACT GCCAGGAATT TGTGAATCAG 
AAAGGACTGT TGCCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC ACTGTCACAT 
GAACCCAAAG TCCTTCAAGA GGGTCTCCTT CAGTTGGACT CCATCCTCTC CTCCCTGGAG 
CCCTTACACC GCCCCATTGA ATCCCCTGGG GGCTCAGTGT TGTTGCGAGA ACTGGCTTGC 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCCCAGGCCA CACCTCTGCT GCATGCACTC 
ACTGCTGCCC ATGCCTACAT CATGATGTTT GTTCATACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCCA TCTCCGTAAA CCAGTGGGGC TCTCAATTGG GTCTGAGTGT TTTGAGCAAG 
CTGAGCCAGT TATACTGTTC CCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTGTGTACC 
CCAAACAGCC TACCATCTGG GTGTGAATTT GGCCAGGCAG ATATGCAGAA ACTGGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGGGA ACAGGATGGA 
GCAGCTGGAA GTATGGATGC TTCTACCCAG GGCTTATTAG AAGGCATTGG GCTAGATGGT 
GACACATTGG CTCCCATGGA GACAGATGAA CCTACTGCTT CAGACTCTAA GGGCAAATCT 
AAAATCACAC CAGCAATGGC TGCCAGAATT AAGCAAATCA AGCCTTTGTT ATCAGCTTCC 
TCCAGATTAG GCCGAGCACT TGCTGAGCTA TTTGGACTTC TTGTTAAACT TTGTGTGGGA 
TCTCCTGTCC GCCAGAGAAG GAGCCATCAT GCTGCCAGCA CCACTACAGC ACCGACACCT 
GCCGCGCGAT CAACAGCCTC AGCTCTCACT AAGCTCTTGA CTAAGGGGTT ATCTTGGCAG 
CCCCCACCAT ATACACCTAC TCCCCGATTC AGGCTGACAT TCTTCATCTG TTCAGTTGGT 
TTCACATCCC CAATGCTGTT TGATGAGAGG AAGTATCCCT ACCACCTCAT GCTGCAAAAA 
TTTCTCTGCT CCGGAGGCCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTGTCCATG 
GGAGGTAAAG TTCCTGTTTC TGAGGGATTG GAACACTCAG ACTTGCCTGA TGGCACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTGGTG GAGAAGATGG TGAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGCTGCC TGCCAAATTG CCTGGAGGTG TCCAGAACTT TCCCCAGTTC 
AGTGCACTGC GCTTCCTTGT GGTAACTCAG AAAGCAGCCT TTACTTGCAT CAAAAACTTA 
TGGAACCGGA AACCCCTGAA GGTATATGGT GGACGAATGG CTGAATCGAT GCTGGCCATT 
CTATGCCACA TCCTCCGAGG AGAACCTGTG ATTCGAGAGA GACTAAGCAA GGAGAAGGAG 
GGGTCTCGAG GAGAAGAGGA TACAGGGCAA GAGGAAGGTG GCTCCCGCCG GGAACCTCAA 
GTCAACCAGC AACAACTGCA ACAGCTCATG GACATGGGCT TCACAAGGGA ACATGCAATG 
GAGGCACTGT TGAACACCAG CACCATGGAG CAGGCCACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TGGGAGGAGT TGTTCGGGAT CTCAGCATGT CTGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAG GAACGGAAAG CTCGGGAAAA GCAGGAGGAG 
GAAGAGGCTA AATGTCTAGA GAAGTTCCAG GATGCTGACC CGTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACTAT GTTGCCAGGC TGCTTCCACC TTCTTGATGA GCTGCCAGAC 
ACAGTATACC GTGTGTGTGA CCTGATCATG ACAGCAATCA AACGTAATGG AGCAGATTAT 
CGTGACATGA TTCTGAAGCA AGTAGTCAAT CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCCCTGAC AACAAGTGAC ACAAAAACCG TGTCAGAGTG GATAAGTCAG 
ATGGCCACAC TGCCCCAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACGCTACTT 
TTTGAGGAGT TGAAGCTACC TTGTGCTTGG GTGGTTGAAT CAAGTGGCAT CCTTAATGTC 
CTAATCAAAC TCTTGGAAGT GGTTCAGCCC TGCCTCCAGG CAGCCAAGGA GCAGAAGGAA 
GTCCAGACCC CAAAGTGGAT CACACCAGTG TTGCTCCTGA TTGATTTCTA TGAAAAGACA 
GCCATCTCCT CAAAAAGGAG AGCCCAGATG ACTAAGTACC TGCAATCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGATCG CTCTGGGCGT TGGTGTAGTT ACAGTGCAAG CAACAATAGC 
ACTATTGATT CTGCCTGGAA ATCTGGAGAG ACAAGCGTGC GATTCACTGC AGGCCGAAGA 
AGATACACGG TCCAATTCAC TACAATGGTG CAGGTTAATG AGGAAACAGG GAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTACCT CGGCTGAATA AAAATTCAAA AAACAGCAAT 
GGACAGGAAC TAGAGAAGAC GCTGGAAGAA AGCAAAGAAA TGGATATCAA ACGTAAAGAA 
AATAAAGGCA ATGATACCCC TTTGGCCCTA GAGAGTACAA ACACTGAAAA GGAGACAAGC 
CTGGAGGAAA CAAAAATCGG GGAGATCCTG ATCCAGGGCT TGACAGAAGA TATGGTGACT 
GTTTTAATCC GGGCCTGCGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA TACTTTGCAT 
GCCACCCTTC GTCTCTGTCT GAGGCTCACC CGGGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACCCGCAT GATCTTGAAT TTGACCCAGA GCTCAGGCTT CAATGGGTTT 
ACTCCCCTGG TCACCCTTCT CTTAAGACAC ATCATTGAGG ACCCCTGTAC CCTTCGTCAT 
ACCATGGAAA AGGTTGTTCG CTCAGCAGCT ACAAGTGGAG CTGGTAGCAC TACCTCTGGT 
GTTGTGTCTG GCAGCCTCGG CTCTCGGGAG ATCAACTACA TCCTTCGTGT CCTTGGGCCA 
GCCGCATGCC GCAATCCAGA CATATTCACA GAAGTGGCCA ACTGCTGTAT CCGCATCGCC 
CTTCCTGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGCCCTA ATGCTGTACA GCTGGTGAAG ACCACCCCTT TGAAGCCCTC ACCTCTGCCT 
GTCATCCCTG ATACTATCAA GGAAGTGATC TATGATATGC TGAATGCTCT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA TAAATCTGAT CCTAAACCTG GGGTTATGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGATGAT GTATACCAGC AGTACCGGTC ACTTACGCGT 
CAGAGCAGTG ACTTTGATAC GCAGTCAGGT TTTTCCATTA ATAGTCAGGT CTTTGCTGCA 
GATGGTGCCT CCACTGAGAC TTCCGCATCT GGGACCTCCC AAGGAGAGGC TTCAACTCCA 
GAGGAGTCTC GAGATGGGAA GAAAGATAAA GAAGGGGACC GGGCCTCTGA GGAAGGCAAA 
CAGAAAGGCA AGGGCAGCAA ACCTTTAATG CCTACCTCCA CTATCCTTCG TCTTCTGGCA 
GAGTTGGTGA GGTCCTATGT TGGTATTGCT ACCCTGATTG CCAACTACAG CTACACTGTG 
GGCCAGTCTG AACTGATCAA AGAGGACTGC AGTGTGCTAG CTTTTGTTCT GGACCACCTG 
CTCCCACATA CCCAGAATGC AGAAGACAAG GACACCCCTG CCTTGGCCCG CCTGTTCCTC 
GCAAGCCTGG CTGCTGCAGG GAGTGGCACA GATGCCCAGG TGGCCCTAGT GAATGAAGTA 
AAAGCAGCCC TTGGACGGGC ACTGGCTATG GCTGAGAGTA CAGAGAAACA TGCCAGGCTT 
CAGGCAGTGA TGTGTATCAT CAGTACTATC ATGGAGTCCT GCCCCTCCAC CTCCAGCTTC 
TACAGCAGTG CCACAGCGAA GACCCAGCAC AATGGCATGA ACAACATCAT TCGGCTTTTC 
CTGAAGAAGG GACTGGTTAA TGACCTGGCC AGAGTACCTC ACAGCTTAGA CCTGTCCAGT 
CCCAACATGG CCAACACAGT CAATGCTGCT CTGAAGCCTT TGGAAACACT TTCCCGGATT 
GTGAACCAGC CCAGTAGCCT TTTTGGCAGC AAGAGTGCTT CTAGCAAGAA CAAGTCTGAG 
CAGGATGCCC AAGGAGCCTC TCAAGATTCC AGTAGCAACC AGCAGGACCC AGGCGAGCCT 
GGGGAAGCAG AAGTGCAGGA GGAGGATCAT GATGTCACTC AGACAGAGGT GGCAGATGGG 
GATATCATGG ATGGGGAGGC TGAAACCGAC TCAGTGGTGA TTGCTGGGCA GCCTGAGGTG 
CTCAGTTCAC AAGAGATGCA GGTTGAGAAT GAGCTGGAGG ACCTGATAGA TGAGTTGCTT 
GAGAGGGATG GCGGATCTGG GAACAGTACA ATTATAGTGA GCAGAAGTGG AGAGGATGAA 
TCACAAGAGG ACGTGCTGAT GGATGAAGCT CCTTCCAACC TCAGCCAAGC TTCCACCTTG 
CAGGCCAACC GAGAAGATTC CATGAATATC CTGGACCCTG AGGATGAGGA GGAGCACACT 
CAGGAAGAGG ACAGCAGTGG CAGTAACGAG GATGAGGATG ATAGTCAGGA TGAAGAGGAG 
GAGGAGGAGG AAGATGAGGA AGATGATCAG GAGGATGATG AAGGTGAAGA GGGAGATGAA 
GACGATGACG ACGATGGCTC TGAGATGGAA TTGGATGAGG ATTATCCTGA TATGAACGCT 
TCTCCCTTGG TCCGATTTGA GCGCTTTGAC CGGGAGGATG ATCTCATCAT TGAGTTTGAC 
AACATGTTCT CCAGTGCTAC AGACATCCCC CCATCCCCAG GAAATATCCC TACCACCCAT 
CCACTGATGG TGCGCCATGC AGACCACAGT TCTCTGACAC TGGGCAGTGG CTCTTCAACA 
ACTCGTCTCA CCCAGGGCAT CGGGCGCAGT CAGAGGACCC TAAGGCAGCT GACGGCCAAT 
ACTGGCCACA CCATTCATGT TCACTACCCT GGGAATCGCC AGCCCAACCC TCCTCTTATA 
CTGCAGAGGT TGCTTGGTCC CTCAGCTGCT GCTGACATCC TTCAGCTGAG CAGCAGCCTT 
CCCCTACAAA GCCGGGGTCG GGCCCGCCTC CTGGTAGGCA ACGATGACGT CCACATCATC 
GCCCGTTCTG ATGATGAGCT GCTGGATGAC TTTTTCCATG ATCAGAGCAC AGCTACCAGC 
CAAGCAGGAA CCCTGTCCAG CATCCCCACA GCCCTGACCC GCTGGACAGA AGAATGCAAA 
GTTCTCGATG CTGAGAGCAT GCATGACTGT GTTTCAGTGG TTAAAGTGTC CATTGTCAAT 
CACCTGGAAT TCCTGAGGGA TGAGGAGCTG GAAGAAAGGC GAGAGAAGCG CAGGAAACAA 
CTGGCTGAGG AAGAAACAAA GATAACTGAT AAAGGCAAAG AAGATAAGGA GAACAGGGAT 
CAGAGTGCAC AGTGTACTGC ATCTAAGTCA AATGACTCCA CTGAACAGAA TCTCTCAGAT 
GGGACGCCTA TGCCTGACAG CTACCCAACA ACCCCATCTT CAACTGATGC AGCTACATCT 
GAGTCCAAGG AGACCCTTGG CACTCTGCAA TCCTCACAAC AGCAACCAAC ACTCCCAACC 
CCACCAGCTT TGGGAGAGGT TCCTCAGGAG CTGCAGTCTC CAGCTGGAGA AGGGGGCAGC 
TCTACACAGC TATTGATGCC TGTAGAGCCA GAGGAATTGG GTCCCACAAG GCCAAGTGGG 
GAAGCAGAAA CAACTCAGAT GGAGTTATCC CCAGCTCCCA CTATAACCTC ACTTTCCCCA 
GAGAGAGCTG AGGATTCTGA TGCACTGACG GCTGTCAGCA GTCAGCTAGA AGGCTCTCCT 
ATGGATACAA GCAGCCTGGC TTCCTGTACC TTAGAGGAGG CTGTGGGTGA CACTTCAGCA 
GCTGGCAGTT CTGAGCAGCC CAGAGCAGGC AGCTCCACTC CTGGGGATGC CCCACCAGCT 
GTGGCGGAAG TGCAAGGCAG GAGTGATGGG TCAGGGGAAT CTGCCCAGCC ACCTGAGGAC 
AGCTCCCCAC CTGCATCCTC TGAGAGCTCT TCCACCAGAG ATTCTGCCGT GGCCATTTCT 
GGAGCAGATT CCCGAGGAAT CCTAGAAGAG CCGTTGCCTT CAACAAGCAG TGAAGAAGAA 
GATCCCCTTG CGGGTATCAG TCTCCCTGAA GGTGTGGACC CCTCTTTTCT GGCTGCCCTG 
CCTGATGACA TCCGTCGGGA AGTTCTACAG AACCAGCTAG GCATTCGTCC ACCAACCCGG 
ACTGCCCCCT CCACAAATAG CTCAGCGCCT GCAGTGGTGG GGAATCCTGG TGTGACTGAA 
GTGAGCCCTG AGTTTCTGGC TGCCCTGCCT CCAGCCATTC AGGAGGAAGT ACTGGCACAG 
CAGAGAGCTG AGCAGCAGCG ACGAGAACTA GCACAGAATG CCAGCTCAGA CACCCCTATG 
GACCCTGTGA CCTTCATCCA GACTCTGCCC TCAGACCTGC GCCGTAGTGT CCTAGAGGAT 
ATGGAGGACA GTGTGTTAGC TGTGATGCCA CCTGACATTG CAGCTGAGGC TCAAGCCCTG 
AGACGAGAGC AAGAAGCCCG GCAGCGACAG CTCATGCATG AGCGTCTGTT TGGGCACAGT 
AGCACCTCCG CACTCTCTGC TATTCTCCGA AGCCCGGCTT TCACCAGTCG CTTAAGTGGC 
AACCGTGGGG TCCAGTATAC TCGCCTTGCT GTGCAGAGAG GTGGCACCTT CCAGATGGGG 
GGTAGCAGCA GCCATAACAG GCCTTCTGGC AGTAATGTAG ATACTCTCCT CCGCCTCCGA 
GGACGGCTCC TTCTGGACCA CGAAGCCCTT TCTTGTCTCT TGGTCCTACT TTTTGTGGAT 
GAGCCAAAGC TCAATACTAG CCGTCTACAC CGAGTACTGA GAAATCTCTG CTACCATGCC 
CAGACCCGCC ACTGGGTCAT CCGCAGTCTG CTCTCCATCT TGCAGCGCAG CAGTGAGAGT 
GAGCTATGCA TTGAAACACC CAAACTCACT ACAAGTGAGG AAAAGGGCAA AAAGTCGAGC 
AAGAGCTGTG GGTCAAGTAG CCATGAGAAC CGTCCCCTGG ACCTGCTACA CAAGATGGAG 
TCAAAGAGCT CCAACCAGCT TTCCTGGCTC TCAGTATCCA TGGATGCAGC CCTAGGCTGC 
AGGACTAATA TATTTCAGAT CCAGCGTTCA GGGGGGCGTA AACATACCGA GAAGCATGCA 
AGCGGTGGCT CCACCGTCCA CATCCATCCC CAAGCTGCTC CTGTTGTCTG CAGACACGTT 
TTGGATACAC TCATTCAATT GGCCAAGGTA TTTCCCAGCC ACTTCACACA GCAGCGGACC 
AAAGAAACAA ACTGTGAGAG TGATCGGGAA AGGGGCAATA AGGCCTGTAG CCCATGCTCC 
TCACAGTCCT CCAGCAGTGG CATTTGCACA GACTTCTGGG ACTTATTGGT AAAACTGGAC 
AACATGAATG TCAGCCGGAA AGGCAAGAAC TCCGTGAAGT CAGTGCCAGT GAGCGCTGGC 
GGTGAGGGGG AAACCTCTCC ATACAGCCTC GAGGCCTCTC CACTGGGGCA GCTCATGAAC 
ATGTTGTCAC ACCCAGTCAT CCGCCGGAGC TCTCTCTTAA CTGAGAAACT CCTCAGACTC 
CTTTCTCTCA TCTCAATTGC TCTCCCAGAA AACAAGGTGT CAGAAGCACA GGCTAATTCT 
GGCAGCGGTG CTTCCTCCAC CACCACTGCC ACCTCAACCA CATCTACCAC CACCACCACT 
GCCGCCTCCA CCACGCCCAC ACCCCCTACT GCACCCACCC CTGTCACTTC TGCTCCAGCC 
CTGGTTGCTG CCACGGCTAT TTCCACCATT GTCGTAGCTG CTTCGACCAC AGTGACTACC 
CCCACGACTG CTACCACTAC TGTTTCAATT TCTCCCACTA CTAAGGGCAG CAAATCTCCA 
GCGAAGGTGA GTGATGGGGG CAGCAGCAGT ACAGACTTTA AGATGGTGTC CTCTGGCCTC 
ACTGAAAACC AGCTACAGCT CTCTGTAGAG GTGTTGACAT CCCACTCTTG TTCTGAGGAA 
GGCTTAGAGG ATGCAGCCAA CGTACTACTG CAGCTCTCCC GGGGGGACTC TGGGACCCGG 
GACACTGTTC TCAAGCTGCT ACTGAATGGA GCCCGCCATC TGGGTTATAC CCTTTGTAAA 
CAAATAGGTA CCCTGCTGGC CGAGCTGCGG GAATACAACC TCGAGCAGCA GCGGCGAGCC 
CAATGTGAAA CCCTCTCTCC TGATGGCCTG CCTGAGGAGC AGCCACAGAC CACCAAGCTG 
AAGGGCAAAA TGCAGAGCAG GTTTGACATG GCTGAGAATG TGGTAATTGT GGCATCTCAG 
AAGCGACCTT TGGGTGGCCG GGAGCTCCAG CTGCCTTCTA TGTCCATGTT GACATCCAAG 
ACATCTACCC AGAAGTTCTT CTTGAGGGTA CTACAGGTCA TCATCCAGCT CCGGGACGAC 
ACGCGCCGGG CTAACAAGAA AGCCAAGCAG ACAGGCAGGC TAGGTTCCTC CGGTTTAGGC 
TCAGCTAGCA GCATCCAGGC AGCTGTTCGG CAGCTGGAGG CTGAGGCTGA TGCCATTATA 
CAAATGGTAC GTGAGGGTCA AAGGGCGCGG AGACAGCAAC AAGCAGCAAC GTCGGAGTCT 
AGCCAGTCAG AGGCGTCTGT CCGGAGGGAG GAATCACCCA TGGATGTGGA CCAGCCATCT 
CCCAGTGCTC AAGATACTCA ATCCATTGCC TCCGATGGAA CCCCACAGGG GGAGAAGGAA 
AAGGAAGAAA GACCACCTGA GTTACCCCTG CTCAGCGAGC AGCTGAGTTT GGACGAGCTG 
TGGGACATGC TTGGGGAGTG TCTAAAGGAA CTAGAGGAAT CCCATGACCA GCATGCGGTG 
CTAGTGCTAC AGCCTGCTGT CGAGGCCTTC TTTCTGGTCC ATGCCACAGA GCGGGAGAGC 
AAGCCTCCTG TCCGAGACAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA CGAGCCTCCT 
CCACTCTCCC CTGCCCCCTT AACCCCAGCC ACGCCTTCCT CCCTTGACCC ATTCTTCTCC 
CGGGAGCCCT CATCTATGCA CATCTCCTCA AGCCTGCCCC CTGACACACA GAAGTTCCTT 
CGCTTTGCAG AGACTCACCG CACTGTGTTA AACCAGATCC TACGGCAGTC CACGACCCAC 
CTTGCTGATG GGCCTTTTGC TGTCCTGGTA GACTACATTC GTGTCCTCGA CTTTGATGTC 
AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG CGTTTAGATG AGGGGCTCCG GAAAGAAGAC 
ATGGCTGTGC ATGTCCGTCG TGACCATGTG TTTGAAGACT CCTATCGTGA GCTGCATCGC 
AAATCCCCCG AAGAAATGAA GAATCGATTG TATATAGTAT TTGAAGGAGA AGAAGGGCAG 
GATGCTGGTG GGCTCCTGCG GGAGTGGTAT ATGATCATCT CTCGAGAGAT GTTTAACCCT 
ATGTATGCCT TGTTCCGTAC CTCACCTGGT GATCGAGTCA CCTACACCAT CAATCCATCT 
TCCCACTGCA ACCCCAACCA CCTCAGCTAC TTCAAGTTTG TCGGACGCAT TGTGGCCAAA 
GCTGTATATG ACAACCGTCT TCTGGAGTGC TACTTTACTC GATCCTTTTA CAAACACATC 
TTGGGCAAGT CAGTCAGATA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAAGGT 
CTGGTTTATC TGCTGGAAAA TGATGTCTCC ACACTAGGCT ATGACCTCAC CTTCAGCACT 
GAGGTCCAAG AGTTTGGAGT TTGTGAAGTT CGTGACCTCA AACCCAATGG GGCCAACATC 
TTGGTAACAG AGGAGAATAA GAAGGAGTAT GTACACCTGG TATGCCAGAT GAGAATGACA 
GGAGCCATCC GCAAGCAGTT GGCGGCTTTC TTAGAAGGCT TCTATGAGAT CATTCCAAAG 
CGCCTCATTT CCATCTTCAC TGAGCAGGAG TTAGAGCTGC TTATATCAGG ACTGCCCACC 
ATTGACATCG ATGATCTGAA ATCCAACACT GAATACCACA AGTACCAGTC CAACTCTATT 
CAGATCCAGT GGTTCTGGAG AGCATTGCGT TCTTTCGATC AAGCTGACCG TGCCAAGTTC 
CTCCAGTTTG TCACAGGTAC TTCCAAGGTA CCCCTGCAAG GCTTTGCTGC CCTCGAAGGC 
ATGAATGGCA TTCAGAAGTT TCAGATCCAT CGAGATGACA GGTCCACAGA TCGCCTGCCT 
TCAGCTCACA CATGTTTTAA TCAGCTGGAT CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC 
CGCCACATGC TACTGTTGGC TATCCAGGAG TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884693.1
CDS	3017..16273
Translation	MDPFISRREQKCMEIGTPRKNGVLVLRSSEAVYHLTTPFQIVCLHLPLSKSKPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029204.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029204.1

ORF Insert Sequence:

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GGAGTCCAGT GTATTCCACA ACGAGCAGCA CTTCTGAAAT CCATGTTGAA TTTCCTCAAG 
AAGGCCATCC AAGACCCTGC TTTCTCAGAT GGCATACGAC ATGTGATGGA TGGTTCTCTG 
CCTACCTCCC TGAAACACAT CATCAGCAAT GCAGAATACT ATGGCCCATC ACTCTTCCTC 
CTAGCTACTG AAGTGGTGAC TGTGTTTGTA TTTCAAGAAC CATCACTGCT CTCCTCACTC 
CAGGACAATG GATTGACAGA TGTCATGCTG CATGCACTGC TTATCAAAGA TGTTCCTGCT 
ACCCGTGAAG TCCTTGGCTC CCTCCCAAAT GTATTCAGTG CACTCTGTTT GAATGCCCGA 
GGTCTTCAGT CTTTTGTTCA GTGTCAGCCT TTTGAACGCC TCTTCAAAGT TCTTCTGTCT 
CCAGATTACC TCCCAGCCAT GCGGAGGAGG AGAAGTTCTG ATCCCCTTGG GGATACTGCA 
TCCAACCTGG GGAGTGCTGT CGATGAGCTC ATGAGACATC AGCCCACCCT TAAAACAGAT 
GCAACGACTG CCATCATCAA GTTACTTGAA GAAATCTGTA ATCTTGGAAG GGACCCCAAA 
TACATCTGTC AGAAGCCATC AATCCAGAAG GCAGATGGCA CTGCCACTGC TCCTCCCCCA 
AGGTCTAATC ATGCCGCAGA AGAAGCCTCT AGTGAGGATG AGGAGGAAGA GGAAGTACAG 
GCCATGCAGA GCTTTAATTC TACCCAGCAA AATGAAACTG AGCCTAATCA GCAGGTTGTT 
GGTACAGAGG AACGTATTCC TATTCCCCTC ATGGATTACA TCCTTAATGT GATGAAATTT 
GTGGAATCTA TTCTGAGCAA CAATACAACA GATGACCACT GCCAGGAATT TGTGAATCAG 
AAAGGACTGT TGCCTTTGGT TACCATTTTG GGTCTTCCCA ATCTGCCCAT TGACTTTCCC 
ACATCTGCTG CCTGTCAGGC TGTTGCAGGT GTCTGCAAAT CCATATTGAC ACTGTCACAT 
GAACCCAAAG TCCTTCAAGA GGGTCTCCTT CAGTTGGACT CCATCCTCTC CTCCCTGGAG 
CCCTTACACC GCCCCATTGA ATCCCCTGGG GGCTCAGTGT TGTTGCGAGA ACTGGCTTGC 
GCAGGCAATG TTGCTGATGC TACCCTCTCA GCCCAGGCCA CACCTCTGCT GCATGCACTC 
ACTGCTGCCC ATGCCTACAT CATGATGTTT GTTCATACTT GCAGAGTTGG ACAGAGTGAA 
ATTCGTTCCA TCTCCGTAAA CCAGTGGGGC TCTCAATTGG GTCTGAGTGT TTTGAGCAAG 
CTGAGCCAGT TATACTGTTC CCTGGTGTGG GAAAGCACTG TCCTCCTCTC TCTGTGTACC 
CCAAACAGCC TACCATCTGG GTGTGAATTT GGCCAGGCAG ATATGCAGAA ACTGGTTCCA 
AAGGATGAGA AGGCAGGTAC GACCCAGGGC GGAAAAAGAT CAGATGGGGA ACAGGATGGA 
GCAGCTGGAA GTATGGATGC TTCTACCCAG GGCTTATTAG AAGGCATTGG GCTAGATGGT 
GACACATTGG CTCCCATGGA GACAGATGAA CCTACTGCTT CAGACTCTAA GGGCAAATCT 
AAAATCACAC CAGCAATGGC TGCCAGAATT AAGCAAATCA AGCCTTTGTT ATCAGCTTCC 
TCCAGATTAG GCCGAGCACT TGCTGAGCTA TTTGGACTTC TTGTTAAACT TTGTGTGGGA 
TCTCCTGTCC GCCAGAGAAG GAGCCATCAT GCTGCCAGCA CCACTACAGC ACCGACACCT 
GCCGCGCGAT CAACAGCCTC AGCTCTCACT AAGCTCTTGA CTAAGGGGTT ATCTTGGCAG 
CCCCCACCAT ATACACCTAC TCCCCGATTC AGGCTGACAT TCTTCATCTG TTCAGTTGGT 
TTCACATCCC CAATGCTGTT TGATGAGAGG AAGTATCCCT ACCACCTCAT GCTGCAAAAA 
TTTCTCTGCT CCGGAGGCCA CAATGCTCTT TTTGAAACTT TCAACTGGGC TCTGTCCATG 
GGAGGTAAAG TTCCTGTTTC TGAGGGATTG GAACACTCAG ACTTGCCTGA TGGCACAGGA 
GAATTCCTAG ATGCCTGGCT TATGCTGGTG GAGAAGATGG TGAATCCCAC CACGGTGCTT 
GAATCTCCAC ATTCGCTGCC TGCCAAATTG CCTGGAGGTG TCCAGAACTT TCCCCAGTTC 
AGTGCACTGC GCTTCCTTGT GGTAACTCAG AAAGCAGCCT TTACTTGCAT CAAAAACTTA 
TGGAACCGGA AACCCCTGAA GGTATATGGT GGACGAATGG CTGAATCGAT GCTGGCCATT 
CTATGCCACA TCCTCCGAGG AGAACCTGTG ATTCGAGAGA GACTAAGCAA GGAGAAGGAG 
GGGTCTCGAG GAGAAGAGGA TACAGGGCAA GAGGAAGGTG GCTCCCGCCG GGAACCTCAA 
GTCAACCAGC AACAACTGCA ACAGCTCATG GACATGGGCT TCACAAGGGA ACATGCAATG 
GAGGCACTGT TGAACACCAG CACCATGGAG CAGGCCACAG AGTACCTTTT AACCCACCCT 
CCTCCAATCA TGGGAGGAGT TGTTCGGGAT CTCAGCATGT CTGAAGAGGA CCAGATGATG 
AGAGCAATTG CTATGTCTCT GGGACAGGAT ATTCCAATGG ATCAAAGGGC AGAGTCACCT 
GAGGAAGTTG CTTGCCGGAA GGAGGAAGAG GAACGGAAAG CTCGGGAAAA GCAGGAGGAG 
GAAGAGGCTA AATGTCTAGA GAAGTTCCAG GATGCTGACC CGTTGGAACA AGATGAGCTC 
CACACTTTCA CAGATACTAT GTTGCCAGGC TGCTTCCACC TTCTTGATGA GCTGCCAGAC 
ACAGTATACC GTGTGTGTGA CCTGATCATG ACAGCAATCA AACGTAATGG AGCAGATTAT 
CGTGACATGA TTCTGAAGCA AGTAGTCAAT CAGGTGTGGG AAGCTGCTGA TGTATTGATC 
AAAGCTGCTC TTCCCCTGAC AACAAGTGAC ACAAAAACCG TGTCAGAGTG GATAAGTCAG 
ATGGCCACAC TGCCCCAGGC CTCCAATTTG GCTACTAGAA TCTTGCTTTT AACGCTACTT 
TTTGAGGAGT TGAAGCTACC TTGTGCTTGG GTGGTTGAAT CAAGTGGCAT CCTTAATGTC 
CTAATCAAAC TCTTGGAAGT GGTTCAGCCC TGCCTCCAGG CAGCCAAGGA GCAGAAGGAA 
GTCCAGACCC CAAAGTGGAT CACACCAGTG TTGCTCCTGA TTGATTTCTA TGAAAAGACA 
GCCATCTCCT CAAAAAGGAG AGCCCAGATG ACTAAGTACC TGCAATCCAA CAGCAACAAC 
TGGCGCTGGT TTGATGATCG CTCTGGGCGT TGGTGTAGTT ACAGTGCAAG CAACAATAGC 
ACTATTGATT CTGCCTGGAA ATCTGGAGAG ACAAGCGTGC GATTCACTGC AGGCCGAAGA 
AGATACACGG TCCAATTCAC TACAATGGTG CAGGTTAATG AGGAAACAGG GAACCGACGC 
CCTGTGATGC TGACTCTCCT CAGGGTACCT CGGCTGAATA AAAATTCAAA AAACAGCAAT 
GGACAGGAAC TAGAGAAGAC GCTGGAAGAA AGCAAAGAAA TGGATATCAA ACGTAAAGAA 
AATAAAGGCA ATGATACCCC TTTGGCCCTA GAGAGTACAA ACACTGAAAA GGAGACAAGC 
CTGGAGGAAA CAAAAATCGG GGAGATCCTG ATCCAGGGCT TGACAGAAGA TATGGTGACT 
GTTTTAATCC GGGCCTGCGT GAGCATGCTG GGAGTCCCTG TGGACCCAGA TACTTTGCAT 
GCCACCCTTC GTCTCTGTCT GAGGCTCACC CGGGACCACA AATATGCCAT GATGTTTGCA 
GAACTGAAGA GTACCCGCAT GATCTTGAAT TTGACCCAGA GCTCAGGCTT CAATGGGTTT 
ACTCCCCTGG TCACCCTTCT CTTAAGACAC ATCATTGAGG ACCCCTGTAC CCTTCGTCAT 
ACCATGGAAA AGGTTGTTCG CTCAGCAGCT ACAAGTGGAG CTGGTAGCAC TACCTCTGGT 
GTTGTGTCTG GCAGCCTCGG CTCTCGGGAG ATCAACTACA TCCTTCGTGT CCTTGGGCCA 
GCCGCATGCC GCAATCCAGA CATATTCACA GAAGTGGCCA ACTGCTGTAT CCGCATCGCC 
CTTCCTGCCC CTCGAGGCTC AGGAACTGCT TCAGATGATG AATTTGAGAA TCTTAGAATT 
AAAGGCCCTA ATGCTGTACA GCTGGTGAAG ACCACCCCTT TGAAGCCCTC ACCTCTGCCT 
GTCATCCCTG ATACTATCAA GGAAGTGATC TATGATATGC TGAATGCTCT GGCTGCATAC 
CATGCTCCAG AGGAAGCAGA TAAATCTGAT CCTAAACCTG GGGTTATGAC CCAAGAGGTT 
GGCCAGCTCC TGCAAGACAT GGGTGATGAT GTATACCAGC AGTACCGGTC ACTTACGCGT 
CAGAGCAGTG ACTTTGATAC GCAGTCAGGT TTTTCCATTA ATAGTCAGGT CTTTGCTGCA 
GATGGTGCCT CCACTGAGAC TTCCGCATCT GGGACCTCCC AAGGAGAGGC TTCAACTCCA 
GAGGAGTCTC GAGATGGGAA GAAAGATAAA GAAGGGGACC GGGCCTCTGA GGAAGGCAAA 
CAGAAAGGCA AGGGCAGCAA ACCTTTAATG CCTACCTCCA CTATCCTTCG TCTTCTGGCA 
GAGTTGGTGA GGTCCTATGT TGGTATTGCT ACCCTGATTG CCAACTACAG CTACACTGTG 
GGCCAGTCTG AACTGATCAA AGAGGACTGC AGTGTGCTAG CTTTTGTTCT GGACCACCTG 
CTCCCACATA CCCAGAATGC AGAAGACAAG GACACCCCTG CCTTGGCCCG CCTGTTCCTC 
GCAAGCCTGG CTGCTGCAGG GAGTGGCACA GATGCCCAGG TGGCCCTAGT GAATGAAGTA 
AAAGCAGCCC TTGGACGGGC ACTGGCTATG GCTGAGAGTA CAGAGAAACA TGCCAGGCTT 
CAGGCAGTGA TGTGTATCAT CAGTACTATC ATGGAGTCCT GCCCCTCCAC CTCCAGCTTC 
TACAGCAGTG CCACAGCGAA GACCCAGCAC AATGGCATGA ACAACATCAT TCGGCTTTTC 
CTGAAGAAGG GACTGGTTAA TGACCTGGCC AGAGTACCTC ACAGCTTAGA CCTGTCCAGT 
CCCAACATGG CCAACACAGT CAATGCTGCT CTGAAGCCTT TGGAAACACT TTCCCGGATT 
GTGAACCAGC CCAGTAGCCT TTTTGGCAGC AAGAGTGCTT CTAGCAAGAA CAAGTCTGAG 
CAGGATGCCC AAGGAGCCTC TCAAGATTCC AGTAGCAACC AGCAGGACCC AGGCGAGCCT 
GGGGAAGCAG AAGTGCAGGA GGAGGATCAT GATGTCACTC AGACAGAGGT GGCAGATGGG 
GATATCATGG ATGGGGAGGC TGAAACCGAC TCAGTGGTGA TTGCTGGGCA GCCTGAGGTG 
CTCAGTTCAC AAGAGATGCA GGTTGAGAAT GAGCTGGAGG ACCTGATAGA TGAGTTGCTT 
GAGAGGGATG GCGGATCTGG GAACAGTACA ATTATAGTGA GCAGAAGTGG AGAGGATGAA 
TCACAAGAGG ACGTGCTGAT GGATGAAGCT CCTTCCAACC TCAGCCAAGC TTCCACCTTG 
CAGGCCAACC GAGAAGATTC CATGAATATC CTGGACCCTG AGGATGAGGA GGAGCACACT 
CAGGAAGAGG ACAGCAGTGG CAGTAACGAG GATGAGGATG ATAGTCAGGA TGAAGAGGAG 
GAGGAGGAGG AAGATGAGGA AGATGATCAG GAGGATGATG AAGGTGAAGA GGGAGATGAA 
GACGATGACG ACGATGGCTC TGAGATGGAA TTGGATGAGG ATTATCCTGA TATGAACGCT 
TCTCCCTTGG TCCGATTTGA GCGCTTTGAC CGGGAGGATG ATCTCATCAT TGAGTTTGAC 
AACATGTTCT CCAGTGCTAC AGACATCCCC CCATCCCCAG GAAATATCCC TACCACCCAT 
CCACTGATGG TGCGCCATGC AGACCACAGT TCTCTGACAC TGGGCAGTGG CTCTTCAACA 
ACTCGTCTCA CCCAGGGCAT CGGGCGCAGT CAGAGGACCC TAAGGCAGCT GACGGCCAAT 
ACTGGCCACA CCATTCATGT TCACTACCCT GGGAATCGCC AGCCCAACCC TCCTCTTATA 
CTGCAGAGGT TGCTTGGTCC CTCAGCTGCT GCTGACATCC TTCAGCTGAG CAGCAGCCTT 
CCCCTACAAA GCCGGGGTCG GGCCCGCCTC CTGGTAGGCA ACGATGACGT CCACATCATC 
GCCCGTTCTG ATGATGAGCT GCTGGATGAC TTTTTCCATG ATCAGAGCAC AGCTACCAGC 
CAAGCAGGAA CCCTGTCCAG CATCCCCACA GCCCTGACCC GCTGGACAGA AGAATGCAAA 
GTTCTCGATG CTGAGAGCAT GCATGACTGT GTTTCAGTGG TTAAAGTGTC CATTGTCAAT 
CACCTGGAAT TCCTGAGGGA TGAGGAGCTG GAAGAAAGGC GAGAGAAGCG CAGGAAACAA 
CTGGCTGAGG AAGAAACAAA GATAACTGAT AAAGGCAAAG AAGATAAGGA GAACAGGGAT 
CAGAGTGCAC AGTGTACTGC ATCTAAGTCA AATGACTCCA CTGAACAGAA TCTCTCAGAT 
GGGACGCCTA TGCCTGACAG CTACCCAACA ACCCCATCTT CAACTGATGC AGCTACATCT 
GAGTCCAAGG AGACCCTTGG CACTCTGCAA TCCTCACAAC AGCAACCAAC ACTCCCAACC 
CCACCAGCTT TGGGAGAGGT TCCTCAGGAG CTGCAGTCTC CAGCTGGAGA AGGGGGCAGC 
TCTACACAGC TATTGATGCC TGTAGAGCCA GAGGAATTGG GTCCCACAAG GCCAAGTGGG 
GAAGCAGAAA CAACTCAGAT GGAGTTATCC CCAGCTCCCA CTATAACCTC ACTTTCCCCA 
GAGAGAGCTG AGGATTCTGA TGCACTGACG GCTGTCAGCA GTCAGCTAGA AGGCTCTCCT 
ATGGATACAA GCAGCCTGGC TTCCTGTACC TTAGAGGAGG CTGTGGGTGA CACTTCAGCA 
GCTGGCAGTT CTGAGCAGCC CAGAGCAGGC AGCTCCACTC CTGGGGATGC CCCACCAGCT 
GTGGCGGAAG TGCAAGGCAG GAGTGATGGG TCAGGGGAAT CTGCCCAGCC ACCTGAGGAC 
AGCTCCCCAC CTGCATCCTC TGAGAGCTCT TCCACCAGAG ATTCTGCCGT GGCCATTTCT 
GGAGCAGATT CCCGAGGAAT CCTAGAAGAG CCGTTGCCTT CAACAAGCAG TGAAGAAGAA 
GATCCCCTTG CGGGTATCAG TCTCCCTGAA GGTGTGGACC CCTCTTTTCT GGCTGCCCTG 
CCTGATGACA TCCGTCGGGA AGTTCTACAG AACCAGCTAG GCATTCGTCC ACCAACCCGG 
ACTGCCCCCT CCACAAATAG CTCAGCGCCT GCAGTGGTGG GGAATCCTGG TGTGACTGAA 
GTGAGCCCTG AGTTTCTGGC TGCCCTGCCT CCAGCCATTC AGGAGGAAGT ACTGGCACAG 
CAGAGAGCTG AGCAGCAGCG ACGAGAACTA GCACAGAATG CCAGCTCAGA CACCCCTATG 
GACCCTGTGA CCTTCATCCA GACTCTGCCC TCAGACCTGC GCCGTAGTGT CCTAGAGGAT 
ATGGAGGACA GTGTGTTAGC TGTGATGCCA CCTGACATTG CAGCTGAGGC TCAAGCCCTG 
AGACGAGAGC AAGAAGCCCG GCAGCGACAG CTCATGCATG AGCGTCTGTT TGGGCACAGT 
AGCACCTCCG CACTCTCTGC TATTCTCCGA AGCCCGGCTT TCACCAGTCG CTTAAGTGGC 
AACCGTGGGG TCCAGTATAC TCGCCTTGCT GTGCAGAGAG GTGGCACCTT CCAGATGGGG 
GGTAGCAGCA GCCATAACAG GCCTTCTGGC AGTAATGTAG ATACTCTCCT CCGCCTCCGA 
GGACGGCTCC TTCTGGACCA CGAAGCCCTT TCTTGTCTCT TGGTCCTACT TTTTGTGGAT 
GAGCCAAAGC TCAATACTAG CCGTCTACAC CGAGTACTGA GAAATCTCTG CTACCATGCC 
CAGACCCGCC ACTGGGTCAT CCGCAGTCTG CTCTCCATCT TGCAGCGCAG CAGTGAGAGT 
GAGCTATGCA TTGAAACACC CAAACTCACT ACAAGTGAGG AAAAGGGCAA AAAGTCGAGC 
AAGAGCTGTG GGTCAAGTAG CCATGAGAAC CGTCCCCTGG ACCTGCTACA CAAGATGGAG 
TCAAAGAGCT CCAACCAGCT TTCCTGGCTC TCAGTATCCA TGGATGCAGC CCTAGGCTGC 
AGGACTAATA TATTTCAGAT CCAGCGTTCA GGGGGGCGTA AACATACCGA GAAGCATGCA 
AGCGGTGGCT CCACCGTCCA CATCCATCCC CAAGCTGCTC CTGTTGTCTG CAGACACGTT 
TTGGATACAC TCATTCAATT GGCCAAGGTA TTTCCCAGCC ACTTCACACA GCAGCGGACC 
AAAGAAACAA ACTGTGAGAG TGATCGGGAA AGGGGCAATA AGGCCTGTAG CCCATGCTCC 
TCACAGTCCT CCAGCAGTGG CATTTGCACA GACTTCTGGG ACTTATTGGT AAAACTGGAC 
AACATGAATG TCAGCCGGAA AGGCAAGAAC TCCGTGAAGT CAGTGCCAGT GAGCGCTGGC 
GGTGAGGGGG AAACCTCTCC ATACAGCCTC GAGGCCTCTC CACTGGGGCA GCTCATGAAC 
ATGTTGTCAC ACCCAGTCAT CCGCCGGAGC TCTCTCTTAA CTGAGAAACT CCTCAGACTC 
CTTTCTCTCA TCTCAATTGC TCTCCCAGAA AACAAGGTGT CAGAAGCACA GGCTAATTCT 
GGCAGCGGTG CTTCCTCCAC CACCACTGCC ACCTCAACCA CATCTACCAC CACCACCACT 
GCCGCCTCCA CCACGCCCAC ACCCCCTACT GCACCCACCC CTGTCACTTC TGCTCCAGCC 
CTGGTTGCTG CCACGGCTAT TTCCACCATT GTCGTAGCTG CTTCGACCAC AGTGACTACC 
CCCACGACTG CTACCACTAC TGTTTCAATT TCTCCCACTA CTAAGGGCAG CAAATCTCCA 
GCGAAGGTGA GTGATGGGGG CAGCAGCAGT ACAGACTTTA AGATGGTGTC CTCTGGCCTC 
ACTGAAAACC AGCTACAGCT CTCTGTAGAG GTGTTGACAT CCCACTCTTG TTCTGAGGAA 
GGCTTAGAGG ATGCAGCCAA CGTACTACTG CAGCTCTCCC GGGGGGACTC TGGGACCCGG 
GACACTGTTC TCAAGCTGCT ACTGAATGGA GCCCGCCATC TGGGTTATAC CCTTTGTAAA 
CAAATAGGTA CCCTGCTGGC CGAGCTGCGG GAATACAACC TCGAGCAGCA GCGGCGAGCC 
CAATGTGAAA CCCTCTCTCC TGATGGCCTG CCTGAGGAGC AGCCACAGAC CACCAAGCTG 
AAGGGCAAAA TGCAGAGCAG GTTTGACATG GCTGAGAATG TGGTAATTGT GGCATCTCAG 
AAGCGACCTT TGGGTGGCCG GGAGCTCCAG CTGCCTTCTA TGTCCATGTT GACATCCAAG 
ACATCTACCC AGAAGTTCTT CTTGAGGGTA CTACAGGTCA TCATCCAGCT CCGGGACGAC 
ACGCGCCGGG CTAACAAGAA AGCCAAGCAG ACAGGCAGGC TAGGTTCCTC CGGTTTAGGC 
TCAGCTAGCA GCATCCAGGC AGCTGTTCGG CAGCTGGAGG CTGAGGCTGA TGCCATTATA 
CAAATGGTAC GTGAGGGTCA AAGGGCGCGG AGACAGCAAC AAGCAGCAAC GTCGGAGTCT 
AGCCAGTCAG AGGCGTCTGT CCGGAGGGAG GAATCACCCA TGGATGTGGA CCAGCCATCT 
CCCAGTGCTC AAGATACTCA ATCCATTGCC TCCGATGGAA CCCCACAGGG GGAGAAGGAA 
AAGGAAGAAA GACCACCTGA GTTACCCCTG CTCAGCGAGC AGCTGAGTTT GGACGAGCTG 
TGGGACATGC TTGGGGAGTG TCTAAAGGAA CTAGAGGAAT CCCATGACCA GCATGCGGTG 
CTAGTGCTAC AGCCTGCTGT CGAGGCCTTC TTTCTGGTCC ATGCCACAGA GCGGGAGAGC 
AAGCCTCCTG TCCGAGACAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA CGAGCCTCCT 
CCACTCTCCC CTGCCCCCTT AACCCCAGCC ACGCCTTCCT CCCTTGACCC ATTCTTCTCC 
CGGGAGCCCT CATCTATGCA CATCTCCTCA AGCCTGCCCC CTGACACACA GAAGTTCCTT 
CGCTTTGCAG AGACTCACCG CACTGTGTTA AACCAGATCC TACGGCAGTC CACGACCCAC 
CTTGCTGATG GGCCTTTTGC TGTCCTGGTA GACTACATTC GTGTCCTCGA CTTTGATGTC 
AAGCGCAAAT ATTTCCGCCA AGAGCTGGAG CGTTTAGATG AGGGGCTCCG GAAAGAAGAC 
ATGGCTGTGC ATGTCCGTCG TGACCATGTG TTTGAAGACT CCTATCGTGA GCTGCATCGC 
AAATCCCCCG AAGAAATGAA GAATCGATTG TATATAGTAT TTGAAGGAGA AGAAGGGCAG 
GATGCTGGTG GGCTCCTGCG GGAGTGGTAT ATGATCATCT CTCGAGAGAT GTTTAACCCT 
ATGTATGCCT TGTTCCGTAC CTCACCTGGT GATCGAGTCA CCTACACCAT CAATCCATCT 
TCCCACTGCA ACCCCAACCA CCTCAGCTAC TTCAAGTTTG TCGGACGCAT TGTGGCCAAA 
GCTGTATATG ACAACCGTCT TCTGGAGTGC TACTTTACTC GATCCTTTTA CAAACACATC 
TTGGGCAAGT CAGTCAGATA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAAGGT 
CTGGTTTATC TGCTGGAAAA TGATGTCTCC ACACTAGGCT ATGACCTCAC CTTCAGCACT 
GAGGTCCAAG AGTTTGGAGT TTGTGAAGTT CGTGACCTCA AACCCAATGG GGCCAACATC 
TTGGTAACAG AGGAGAATAA GAAGGAGTAT GTACACCTGG TATGCCAGAT GAGAATGACA 
GGAGCCATCC GCAAGCAGTT GGCGGCTTTC TTAGAAGGCT TCTATGAGAT CATTCCAAAG 
CGCCTCATTT CCATCTTCAC TGAGCAGGAG TTAGAGCTGC TTATATCAGG ACTGCCCACC 
ATTGACATCG ATGATCTGAA ATCCAACACT GAATACCACA AGTACCAGTC CAACTCTATT 
CAGATCCAGT GGTTCTGGAG AGCATTGCGT TCTTTCGATC AAGCTGACCG TGCCAAGTTC 
CTCCAGTTTG TCACAGGTAC TTCCAAGGTA CCCCTGCAAG GCTTTGCTGC CCTCGAAGGC 
ATGAATGGCA TTCAGAAGTT TCAGATCCAT CGAGATGACA GGTCCACAGA TCGCCTGCCT 
TCAGCTCACA CATGTTTTAA TCAGCTGGAT CTGCCTGCCT ATGAGAGCTT TGAGAAGCTC 
CGCCACATGC TACTGTTGGC TATCCAGGAG TGCTCTGAAG GCTTTGGGCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029205.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884694.1 Homo sapiens HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X17, mRNA.

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CloneID	OHu99121
Clone ID Related Accession (Same CDS sequence)	XM_017029205.1
Accession Version	XM_017029205.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTATTC CAATGCCTTG CAGGAATCAG CAAACAGTAT CTTGTATAAT 
GGCTTGATAG AGGAGTTGGT AGATGTCCTT CAGATAACGG ATAAGCAGCT TATGGAGATT 
AAAGCAGCTT CTTTACGAAC ATTAACATCA ATTGTCCACT TGGAGAGAAC TCCCAAACTC 
AGCAGTATTA TTGACTGTAC TGGAACTGCC TCCTACCATG GATTTTTGCC AGTGCTTGTA 
AGGAACTGTA TCCAGGCCAT GATTGATCCT TCCATGGATC CATACCCTCA CCAGTTTGCC 
ACTGCTCTCT TCTCTTTTTT ATACCATCTG GCCAGCTACG ATGCTGGTGG TGAAGCCTTG 
GTCTCCTGTG GAATGATGGA AGCCTTATTG AAGGTCATAA AGTTTCTTGG CGATGAACAG 
GACCAGATAA CATTTGTCAC CAGAGCCGTC AGAGTGGTTG ACCTTATCAC CAACCTGGAT 
ATGGCAGCTT TTCAATCCCA TAGTGGACTT TCTATCTTCA TTTATAGACT TGAGCATGAA 
GTAGATTTGT GCCGAAAAGA ATGTCCGTTT GTGATCAAGC CAAAGATCCA GAGACCCAAT 
ACTACACAAG AAGGAGAGGA AATGGAAACT GATATGGATG GAGTCCAGTG TATTCCACAA 
CGAGCAGCAC TTCTGAAATC CATGTTGAAT TTCCTCAAGA AGGCCATCCA AGACCCTGCT 
TTCTCAGATG GCATACGACA TGTGATGGAT GGTTCTCTGC CTACCTCCCT GAAACACATC 
ATCAGCAATG CAGAATACTA TGGCCCATCA CTCTTCCTCC TAGCTACTGA AGTGGTGACT 
GTGTTTGTAT TTCAAGAACC ATCACTGCTC TCCTCACTCC AGGACAATGG ATTGACAGAT 
GTCATGCTGC ATGCACTGCT TATCAAAGAT GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC 
CTCCCAAATG TATTCAGTGC ACTCTGTTTG AATGCCCGAG GTCTTCAGTC TTTTGTTCAG 
TGTCAGCCTT TTGAACGCCT CTTCAAAGTT CTTCTGTCTC CAGATTACCT CCCAGCCATG 
CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG GATACTGCAT CCAACCTGGG GAGTGCTGTC 
GATGAGCTCA TGAGACATCA GCCCACCCTT AAAACAGATG CAACGACTGC CATCATCAAG 
TTACTTGAAG AAATCTGTAA TCTTGGAAGG GACCCCAAAT ACATCTGTCA GAAGCCATCA 
ATCCAGAAGG CAGATGGCAC TGCCACTGCT CCTCCCCCAA GGTCTAATCA TGCCGCAGAA 
GAAGCCTCTA GTGAGGATGA GGAGGAAGAG GAAGTACAGG CCATGCAGAG CTTTAATTCT 
ACCCAGCAAA ATGAAACTGA GCCTAATCAG CAGGTTGTTG GTACAGAGGA ACGTATTCCT 
ATTCCCCTCA TGGATTACAT CCTTAATGTG ATGAAATTTG TGGAATCTAT TCTGAGCAAC 
AATACAACAG ATGACCACTG CCAGGAATTT GTGAATCAGA AAGGACTGTT GCCTTTGGTT 
ACCATTTTGG GTCTTCCCAA TCTGCCCATT GACTTTCCCA CATCTGCTGC CTGTCAGGCT 
GTTGCAGGTG TCTGCAAATC CATATTGACA CTGTCACATG AACCCAAAGT CCTTCAAGAG 
GGTCTCCTTC AGTTGGACTC CATCCTCTCC TCCCTGGAGC CCTTACACCG CCCCATTGAA 
TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA CTGGCTTGCG CAGGCAATGT TGCTGATGCT 
ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG CATGCACTCA CTGCTGCCCA TGCCTACATC 
ATGATGTTTG TTCATACTTG CAGAGTTGGA CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC 
CAGTGGGGCT CTCAATTGGG TCTGAGTGTT TTGAGCAAGC TGAGCCAGTT ATACTGTTCC 
CTGGTGTGGG AAAGCACTGT CCTCCTCTCT CTGTGTACCC CAAACAGCCT ACCATCTGGG 
TGTGAATTTG GCCAGGCAGA TATGCAGAAA CTGGTTCCAA AGGATGAGAA GGCAGGTACG 
ACCCAGGGCG GAAAAAGATC AGATGGGGAA CAGGATGGAG CAGCTGGAAG TATGGATGCT 
TCTACCCAGG GCTTATTAGA AGGCATTGGG CTAGATGGTG ACACATTGGC TCCCATGGAG 
ACAGATGAAC CTACTGCTTC AGACTCTAAG GGCAAATCTA AAATCACACC AGCAATGGCT 
GCCAGAATTA AGCAAATCAA GCCTTTGTTA TCAGCTTCCT CCAGATTAGG CCGAGCACTT 
GCTGAGCTAT TTGGACTTCT TGTTAAACTT TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG 
AGCCATCATG CTGCCAGCAC CACTACAGCA CCGACACCTG CCGCGCGATC AACAGCCTCA 
GCTCTCACTA AGCTCTTGAC TAAGGGGTTA TCTTGGCAGC CCCCACCATA TACACCTACT 
CCCCGATTCA GGCTGACATT CTTCATCTGT TCAGTTGGTT TCACATCCCC AATGCTGTTT 
GATGAGAGGA AGTATCCCTA CCACCTCATG CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC 
AATGCTCTTT TTGAAACTTT CAACTGGGCT CTGTCCATGG GAGGTAAAGT TCCTGTTTCT 
GAGGGATTGG AACACTCAGA CTTGCCTGAT GGCACAGGAG AATTCCTAGA TGCCTGGCTT 
ATGCTGGTGG AGAAGATGGT GAATCCCACC ACGGTGCTTG AATCTCCACA TTCGCTGCCT 
GCCAAATTGC CTGGAGGTGT CCAGAACTTT CCCCAGTTCA GTGCACTGCG CTTCCTTGTG 
GTAACTCAGA AAGCAGCCTT TACTTGCATC AAAAACTTAT GGAACCGGAA ACCCCTGAAG 
GTATATGGTG GACGAATGGC TGAATCGATG CTGGCCATTC TATGCCACAT CCTCCGAGGA 
GAACCTGTGA TTCGAGAGAG ACTAAGCAAG GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT 
ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG GAACCTCAAG TCAACCAGCA ACAACTGCAA 
CAGCTCATGG ACATGGGCTT CACAAGGGAA CATGCAATGG AGGCACTGTT GAACACCAGC 
ACCATGGAGC AGGCCACAGA GTACCTTTTA ACCCACCCTC CTCCAATCAT GGGAGGAGTT 
GTTCGGGATC TCAGCATGTC TGAAGAGGAC CAGATGATGA GAGCAATTGC TATGTCTCTG 
GGACAGGATA TTCCAATGGA TCAAAGGGCA GAGTCACCTG AGGAAGTTGC TTGCCGGAAG 
GAGGAAGAGG AACGGAAAGC TCGGGAAAAG CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG 
AAGTTCCAGG ATGCTGACCC GTTGGAACAA GATGAGCTCC ACACTTTCAC AGATACTATG 
TTGCCAGGCT GCTTCCACCT TCTTGATGAG CTGCCAGACA CAGTATACCG TGTGTGTGAC 
CTGATCATGA CAGCAATCAA ACGTAATGGA GCAGATTATC GTGACATGAT TCTGAAGCAA 
GTAGTCAATC AGGTGTGGGA AGCTGCTGAT GTATTGATCA AAGCTGCTCT TCCCCTGACA 
ACAAGTGACA CAAAAACCGT GTCAGAGTGG ATAAGTCAGA TGGCCACACT GCCCCAGGCC 
TCCAATTTGG CTACTAGAAT CTTGCTTTTA ACGCTACTTT TTGAGGAGTT GAAGCTACCT 
TGTGCTTGGG TGGTTGAATC AAGTGGCATC CTTAATGTCC TAATCAAACT CTTGGAAGTG 
GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG CAGAAGGAAG TCCAGACCCC AAAGTGGATC 
ACACCAGTGT TGCTCCTGAT TGATTTCTAT GAAAAGACAG CCATCTCCTC AAAAAGGAGA 
GCCCAGATGA CTAAGTACCT GCAATCCAAC AGCAACAACT GGCGCTGGTT TGATGATCGC 
TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC AACAATAGCA CTATTGATTC TGCCTGGAAA 
TCTGGAGAGA CAAGCGTGCG ATTCACTGCA GGCCGAAGAA GATACACGGT CCAATTCACT 
ACAATGGTGC AGGTTAATGA GGAAACAGGG AACCGACGCC CTGTGATGCT GACTCTCCTC 
AGGGTACCTC GGCTGAATAA AAATTCAAAA AACAGCAATG GACAGGAACT AGAGAAGACG 
CTGGAAGAAA GCAAAGAAAT GGATATCAAA CGTAAAGAAA ATAAAGGCAA TGATACCCCT 
TTGGCCCTAG AGAGTACAAA CACTGAAAAG GAGACAAGCC TGGAGGAAAC AAAAATCGGG 
GAGATCCTGA TCCAGGGCTT GACAGAAGAT ATGGTGACTG TTTTAATCCG GGCCTGCGTG 
AGCATGCTGG GAGTCCCTGT GGACCCAGAT ACTTTGCATG CCACCCTTCG TCTCTGTCTG 
AGGCTCACCC GGGACCACAA ATATGCCATG ATGTTTGCAG AACTGAAGAG TACCCGCATG 
ATCTTGAATT TGACCCAGAG CTCAGGCTTC AATGGGTTTA CTCCCCTGGT CACCCTTCTC 
TTAAGACACA TCATTGAGGA CCCCTGTACC CTTCGTCATA CCATGGAAAA GGTTGTTCGC 
TCAGCAGCTA CAAGTGGAGC TGGTAGCACT ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC 
TCTCGGGAGA TCAACTACAT CCTTCGTGTC CTTGGGCCAG CCGCATGCCG CAATCCAGAC 
ATATTCACAG AAGTGGCCAA CTGCTGTATC CGCATCGCCC TTCCTGCCCC TCGAGGCTCA 
GGAACTGCTT CAGATGATGA ATTTGAGAAT CTTAGAATTA AAGGCCCTAA TGCTGTACAG 
CTGGTGAAGA CCACCCCTTT GAAGCCCTCA CCTCTGCCTG TCATCCCTGA TACTATCAAG 
GAAGTGATCT ATGATATGCT GAATGCTCTG GCTGCATACC ATGCTCCAGA GGAAGATAAA 
TCTGATCCTA AACCTGGGGT TATGACCCAA GAGGTTGGCC AGCTCCTGCA AGACATGGGT 
GATGATGTAT ACCAGCAGTA CCGGTCACTT ACGCGTCAGA GCAGTGACTT TGATACGCAG 
TCAGGTTTTT CCATTAATAG TCAGGTCTTT GCTGCAGATG GTGCCTCCAC TGAGACTTCC 
GCATCTGGGA CCTCCCAAGG AGAGGCTTCA ACTCCAGAGG AGTCTCGAGA TGGGAAGAAA 
GATAAAGAAG GGGACCGGGC CTCTGAGGAA GGCAAACAGA AAGGCAAGGG CAGCAAACCT 
TTAATGCCTA CCTCCACTAT CCTTCGTCTT CTGGCAGAGT TGGTGAGGTC CTATGTTGGT 
ATTGCTACCC TGATTGCCAA CTACAGCTAC ACTGTGGGCC AGTCTGAACT GATCAAAGAG 
GACTGCAGTG TGCTAGCTTT TGTTCTGGAC CACCTGCTCC CACATACCCA GAATGCAGAA 
GACAAGGACA CCCCTGCCTT GGCCCGCCTG TTCCTCGCAA GCCTGGCTGC TGCAGGGAGT 
GGCACAGATG CCCAGGTGGC CCTAGTGAAT GAAGTAAAAG CAGCCCTTGG ACGGGCACTG 
GCTATGGCTG AGAGTACAGA GAAACATGCC AGGCTTCAGG CAGTGATGTG TATCATCAGT 
ACTATCATGG AGTCCTGCCC CTCCACCTCC AGCTTCTACA GCAGTGCCAC AGCGAAGACC 
CAGCACAATG GCATGAACAA CATCATTCGG CTTTTCCTGA AGAAGGGACT GGTTAATGAC 
CTGGCCAGAG TACCTCACAG CTTAGACCTG TCCAGTCCCA ACATGGCCAA CACAGTCAAT 
GCTGCTCTGA AGCCTTTGGA AACACTTTCC CGGATTGTGA ACCAGCCCAG TAGCCTTTTT 
GGCAGCAAGA GTGCTTCTAG CAAGAACAAG TCTGAGCAGG ATGCCCAAGG AGCCTCTCAA 
GATTCCAGTA GCAACCAGCA GGACCCAGGC GAGCCTGGGG AAGCAGAAGT GCAGGAGGAG 
GATCATGATG TCACTCAGAC AGAGGTGGCA GATGGGGATA TCATGGATGG GGAGGCTGAA 
ACCGACTCAG TGGTGATTGC TGGGCAGCCT GAGGTGCTCA GTTCACAAGA GATGCAGGTT 
GAGAATGAGC TGGAGGACCT GATAGATGAG TTGCTTGAGA GGGATGGCGG ATCTGGGAAC 
AGTACAATTA TAGATTCCAT GAATATCCTG GACCCTGAGG ATGAGGAGGA GCACACTCAG 
GAAGAGGACA GCAGTGGCAG TAACGAGGAT GAGGATGATA GTCAGGATGA AGAGGAGGAG 
GAGGAGGAAG ATGAGGAAGA TGATCAGGAG GATGATGAAG GTGAAGAGGG AGATGAAGAC 
GATGACGACG ATGGCTCTGA GATGGAATTG GATGAGGATT ATCCTGATAT GAACGCTTCT 
CCCTTGGTCC GATTTGAGCG CTTTGACCGG GAGGATGATC TCATCATTGA GTTTGACAAC 
ATGTTCTCCA GTGCTACAGA CATCCCCCCA TCCCCAGGAA ATATCCCTAC CACCCATCCA 
CTGATGGTGC GCCATGCAGA CCACAGTTCT CTGACACTGG GCAGTGGCTC TTCAACAACT 
CGTCTCACCC AGGGCATCGG GCGCAGTCAG AGGACCCTAA GGCAGCTGAC GGCCAATACT 
GGCCACACCA TTCATGTTCA CTACCCTGGG AATCGCCAGC CCAACCCTCC TCTTATACTG 
CAGAGGTTGC TTGGTCCCTC AGCTGCTGCT GACATCCTTC AGCTGAGCAG CAGCCTTCCC 
CTACAAAGCC GGGGTCGGGC CCGCCTCCTG GTAGGCAACG ATGACGTCCA CATCATCGCC 
CGTTCTGATG ATGAGCTGCT GGATGACTTT TTCCATGATC AGAGCACAGC TACCAGCCAA 
GCAGGAACCC TGTCCAGCAT CCCCACAGCC CTGACCCGCT GGACAGAAGA ATGCAAAGTT 
CTCGATGCTG AGAGCATGCA TGACTGTGTT TCAGTGGTTA AAGTGTCCAT TGTCAATCAC 
CTGGAATTCC TGAGGGATGA GGAGCTGGAA GAAAGGCGAG AGAAGCGCAG GAAACAACTG 
GCTGAGGAAG AAACAAAGAT AACTGATAAA GGCAAAGAAG ATAAGGAGAA CAGGGATCAG 
AGTGCACAGT GTACTGCATC TAAGTCAAAT GACTCCACTG AACAGAATCT CTCAGATGGG 
ACGCCTATGC CTGACAGCTA CCCAACAACC CCATCTTCAA CTGATGCAGC TACATCTGAG 
TCCAAGGAGA CCCTTGGCAC TCTGCAATCC TCACAACAGC AACCAACACT CCCAACCCCA 
CCAGCTTTGG GAGAGGTTCC TCAGGAGCTG CAGTCTCCAG CTGGAGAAGG GGGCAGCTCT 
ACACAGCTAT TGATGCCTGT AGAGCCAGAG GAATTGGGTC CCACAAGGCC AAGTGGGGAA 
GCAGAAACAA CTCAGATGGA GTTATCCCCA GCTCCCACTA TAACCTCACT TTCCCCAGAG 
AGAGCTGAGG ATTCTGATGC ACTGACGGCT GTCAGCAGTC AGCTAGAAGG CTCTCCTATG 
GATACAAGCA GCCTGGCTTC CTGTACCTTA GAGGAGGCTG TGGGTGACAC TTCAGCAGCT 
GGCAGTTCTG AGCAGCCCAG AGCAGGCAGC TCCACTCCTG GGGATGCCCC ACCAGCTGTG 
GCGGAAGTGC AAGGCAGGAG TGATGGGTCA GGGGAATCTG CCCAGCCACC TGAGGACAGC 
TCCCCACCTG CATCCTCTGA GAGCTCTTCC ACCAGAGATT CTGCCGTGGC CATTTCTGGA 
GCAGATTCCC GAGGAATCCT AGAAGAGCCG TTGCCTTCAA CAAGCAGTGA AGAAGAAGAT 
CCCCTTGCGG GTATCAGTCT CCCTGAAGGT GTGGACCCCT CTTTTCTGGC TGCCCTGCCT 
GATGACATCC GTCGGGAAGT TCTACAGAAC CAGCTAGGCA TTCGTCCACC AACCCGGACT 
GCCCCCTCCA CAAATAGCTC AGCGCCTGCA GTGGTGGGGA ATCCTGGTGT GACTGAAGTG 
AGCCCTGAGT TTCTGGCTGC CCTGCCTCCA GCCATTCAGG AGGAAGTACT GGCACAGCAG 
AGAGCTGAGC AGCAGCGACG AGAACTAGCA CAGAATGCCA GCTCAGACAC CCCTATGGAC 
CCTGTGACCT TCATCCAGAC TCTGCCCTCA GACCTGCGCC GTAGTGTCCT AGAGGATATG 
GAGGACAGTG TGTTAGCTGT GATGCCACCT GACATTGCAG CTGAGGCTCA AGCCCTGAGA 
CGAGAGCAAG AAGCCCGGCA GCGACAGCTC ATGCATGAGC GTCTGTTTGG GCACAGTAGC 
ACCTCCGCAC TCTCTGCTAT TCTCCGAAGC CCGGCTTTCA CCAGTCGCTT AAGTGGCAAC 
CGTGGGGTCC AGTATACTCG CCTTGCTGTG CAGAGAGGTG GCACCTTCCA GATGGGGGGT 
AGCAGCAGCC ATAACAGGCC TTCTGGCAGT AATGTAGATA CTCTCCTCCG CCTCCGAGGA 
CGGCTCCTTC TGGACCACGA AGCCCTTTCT TGTCTCTTGG TCCTACTTTT TGTGGATGAG 
CCAAAGCTCA ATACTAGCCG TCTACACCGA GTACTGAGAA ATCTCTGCTA CCATGCCCAG 
ACCCGCCACT GGGTCATCCG CAGTCTGCTC TCCATCTTGC AGCGCAGCAG TGAGAGTGAG 
CTATGCATTG AAACACCCAA ACTCACTACA AGTGAGGAAA AGGGCAAAAA GTCGAGCAAG 
AGCTGTGGGT CAAGTAGCCA TGAGAACCGT CCCCTGGACC TGCTACACAA GATGGAGTCA 
AAGAGCTCCA ACCAGCTTTC CTGGCTCTCA GTATCCATGG ATGCAGCCCT AGGCTGCAGG 
ACTAATATAT TTCAGATCCA GCGTTCAGGG GGGCGTAAAC ATACCGAGAA GCATGCAAGC 
GGTGGCTCCA CCGTCCACAT CCATCCCCAA GCTGCTCCTG TTGTCTGCAG ACACGTTTTG 
GATACACTCA TTCAATTGGC CAAGGTATTT CCCAGCCACT TCACACAGCA GCGGACCAAA 
GAAACAAACT GTGAGAGTGA TCGGGAAAGG GGCAATAAGG CCTGTAGCCC ATGCTCCTCA 
CAGTCCTCCA GCAGTGGCAT TTGCACAGAC TTCTGGGACT TATTGGTAAA ACTGGACAAC 
ATGAATGTCA GCCGGAAAGG CAAGAACTCC GTGAAGTCAG TGCCAGTGAG CGCTGGCGGT 
GAGGGGGAAA CCTCTCCATA CAGCCTCGAG GCCTCTCCAC TGGGGCAGCT CATGAACATG 
TTGTCACACC CAGTCATCCG CCGGAGCTCT CTCTTAACTG AGAAACTCCT CAGACTCCTT 
TCTCTCATCT CAATTGCTCT CCCAGAAAAC AAGGTGTCAG AAGCACAGGC TAATTCTGGC 
AGCGGTGCTT CCTCCACCAC CACTGCCACC TCAACCACAT CTACCACCAC CACCACTGCC 
GCCTCCACCA CGCCCACACC CCCTACTGCA CCCACCCCTG TCACTTCTGC TCCAGCCCTG 
GTTGCTGCCA CGGCTATTTC CACCATTGTC GTAGCTGCTT CGACCACAGT GACTACCCCC 
ACGACTGCTA CCACTACTGT TTCAATTTCT CCCACTACTA AGGGCAGCAA ATCTCCAGCG 
AAGGTGAGTG ATGGGGGCAG CAGCAGTACA GACTTTAAGA TGGTGTCCTC TGGCCTCACT 
GAAAACCAGC TACAGCTCTC TGTAGAGGTG TTGACATCCC ACTCTTGTTC TGAGGAAGGC 
TTAGAGGATG CAGCCAACGT ACTACTGCAG CTCTCCCGGG GGGACTCTGG GACCCGGGAC 
ACTGTTCTCA AGCTGCTACT GAATGGAGCC CGCCATCTGG GTTATACCCT TTGTAAACAA 
ATAGGTACCC TGCTGGCCGA GCTGCGGGAA TACAACCTCG AGCAGCAGCG GCGAGCCCAA 
TGTGAAACCC TCTCTCCTGA TGGCCTGCCT GAGGAGCAGC CACAGACCAC CAAGCTGAAG 
GGCAAAATGC AGAGCAGGTT TGACATGGCT GAGAATGTGG TAATTGTGGC ATCTCAGAAG 
CGACCTTTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA 
TCTACCCAGA AGTTCTTCTT GAGGGTACTA CAGGTCATCA TCCAGCTCCG GGACGACACG 
CGCCGGGCTA ACAAGAAAGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA 
GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA 
ATGGTACGTG AGGGTCAAAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCTAGC 
CAGTCAGAGG CGTCTGTCCG GAGGGAGGAA TCACCCATGG ATGTGGACCA GCCATCTCCC 
AGTGCTCAAG ATACTCAATC CATTGCCTCC GATGGAACCC CACAGGGGGA GAAGGAAAAG 
GAAGAAAGAC CACCTGAGTT ACCCCTGCTC AGCGAGCAGC TGAGTTTGGA CGAGCTGTGG 
GACATGCTTG GGGAGTGTCT AAAGGAACTA GAGGAATCCC ATGACCAGCA TGCGGTGCTA 
GTGCTACAGC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CCACAGAGCG GGAGAGCAAG 
CCTCCTGTCC GAGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCTCCTCCA 
CTCTCCCCTG CCCCCTTAAC CCCAGCCACG CCTTCCTCCC TTGACCCATT CTTCTCCCGG 
GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTCCTTCGC 
TTTGCAGAGA CTCACCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC GACCCACCTT 
GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT TGATGTCAAG 
CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTAGATGAGG GGCTCCGGAA AGAAGACATG 
GCTGTGCATG TCCGTCGTGA CCATGTGTTT GAAGACTCCT ATCGTGAGCT GCATCGCAAA 
TCCCCCGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT 
GCTGGTGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG 
TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGAGTCACCT ACACCATCAA TCCATCTTCC 
CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTCG GACGCATTGT GGCCAAAGCT 
GTATATGACA ACCGTCTTCT GGAGTGCTAC TTTACTCGAT CCTTTTACAA ACACATCTTG 
GGCAAGTCAG TCAGATATAC AGATATGGAG AGTGAAGATT ACCACTTCTA CCAAGGTCTG 
GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ACCTCACCTT CAGCACTGAG 
GTCCAAGAGT TTGGAGTTTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG 
GTAACAGAGG AGAATAAGAA GGAGTATGTA CACCTGGTAT GCCAGATGAG AATGACAGGA 
GCCATCCGCA AGCAGTTGGC GGCTTTCTTA GAAGGCTTCT ATGAGATCAT TCCAAAGCGC 
CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTTA TATCAGGACT GCCCACCATT 
GACATCGATG ATCTGAAATC CAACACTGAA TACCACAAGT ACCAGTCCAA CTCTATTCAG 
ATCCAGTGGT TCTGGAGAGC ATTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC 
CAGTTTGTCA CAGGTACTTC CAAGGTACCC CTGCAAGGCT TTGCTGCCCT CGAAGGCATG 
AATGGCATTC AGAAGTTTCA GATCCATCGA GATGACAGGT CCACAGATCG CCTGCCTTCA 
GCTCACACAT GTTTTAATCA GCTGGATCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC 
CACATGCTAC TGTTGGCTAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884694.1
CDS	3018..16151
Translation	MDPFISRREQKCMEIGTPRKNGVLVLRSSEAVYHLTTPFQIVCLHLPLSKSKPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEDKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029205.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029205.1

ORF Insert Sequence:

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTATTC CAATGCCTTG CAGGAATCAG CAAACAGTAT CTTGTATAAT 
GGCTTGATAG AGGAGTTGGT AGATGTCCTT CAGATAACGG ATAAGCAGCT TATGGAGATT 
AAAGCAGCTT CTTTACGAAC ATTAACATCA ATTGTCCACT TGGAGAGAAC TCCCAAACTC 
AGCAGTATTA TTGACTGTAC TGGAACTGCC TCCTACCATG GATTTTTGCC AGTGCTTGTA 
AGGAACTGTA TCCAGGCCAT GATTGATCCT TCCATGGATC CATACCCTCA CCAGTTTGCC 
ACTGCTCTCT TCTCTTTTTT ATACCATCTG GCCAGCTACG ATGCTGGTGG TGAAGCCTTG 
GTCTCCTGTG GAATGATGGA AGCCTTATTG AAGGTCATAA AGTTTCTTGG CGATGAACAG 
GACCAGATAA CATTTGTCAC CAGAGCCGTC AGAGTGGTTG ACCTTATCAC CAACCTGGAT 
ATGGCAGCTT TTCAATCCCA TAGTGGACTT TCTATCTTCA TTTATAGACT TGAGCATGAA 
GTAGATTTGT GCCGAAAAGA ATGTCCGTTT GTGATCAAGC CAAAGATCCA GAGACCCAAT 
ACTACACAAG AAGGAGAGGA AATGGAAACT GATATGGATG GAGTCCAGTG TATTCCACAA 
CGAGCAGCAC TTCTGAAATC CATGTTGAAT TTCCTCAAGA AGGCCATCCA AGACCCTGCT 
TTCTCAGATG GCATACGACA TGTGATGGAT GGTTCTCTGC CTACCTCCCT GAAACACATC 
ATCAGCAATG CAGAATACTA TGGCCCATCA CTCTTCCTCC TAGCTACTGA AGTGGTGACT 
GTGTTTGTAT TTCAAGAACC ATCACTGCTC TCCTCACTCC AGGACAATGG ATTGACAGAT 
GTCATGCTGC ATGCACTGCT TATCAAAGAT GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC 
CTCCCAAATG TATTCAGTGC ACTCTGTTTG AATGCCCGAG GTCTTCAGTC TTTTGTTCAG 
TGTCAGCCTT TTGAACGCCT CTTCAAAGTT CTTCTGTCTC CAGATTACCT CCCAGCCATG 
CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG GATACTGCAT CCAACCTGGG GAGTGCTGTC 
GATGAGCTCA TGAGACATCA GCCCACCCTT AAAACAGATG CAACGACTGC CATCATCAAG 
TTACTTGAAG AAATCTGTAA TCTTGGAAGG GACCCCAAAT ACATCTGTCA GAAGCCATCA 
ATCCAGAAGG CAGATGGCAC TGCCACTGCT CCTCCCCCAA GGTCTAATCA TGCCGCAGAA 
GAAGCCTCTA GTGAGGATGA GGAGGAAGAG GAAGTACAGG CCATGCAGAG CTTTAATTCT 
ACCCAGCAAA ATGAAACTGA GCCTAATCAG CAGGTTGTTG GTACAGAGGA ACGTATTCCT 
ATTCCCCTCA TGGATTACAT CCTTAATGTG ATGAAATTTG TGGAATCTAT TCTGAGCAAC 
AATACAACAG ATGACCACTG CCAGGAATTT GTGAATCAGA AAGGACTGTT GCCTTTGGTT 
ACCATTTTGG GTCTTCCCAA TCTGCCCATT GACTTTCCCA CATCTGCTGC CTGTCAGGCT 
GTTGCAGGTG TCTGCAAATC CATATTGACA CTGTCACATG AACCCAAAGT CCTTCAAGAG 
GGTCTCCTTC AGTTGGACTC CATCCTCTCC TCCCTGGAGC CCTTACACCG CCCCATTGAA 
TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA CTGGCTTGCG CAGGCAATGT TGCTGATGCT 
ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG CATGCACTCA CTGCTGCCCA TGCCTACATC 
ATGATGTTTG TTCATACTTG CAGAGTTGGA CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC 
CAGTGGGGCT CTCAATTGGG TCTGAGTGTT TTGAGCAAGC TGAGCCAGTT ATACTGTTCC 
CTGGTGTGGG AAAGCACTGT CCTCCTCTCT CTGTGTACCC CAAACAGCCT ACCATCTGGG 
TGTGAATTTG GCCAGGCAGA TATGCAGAAA CTGGTTCCAA AGGATGAGAA GGCAGGTACG 
ACCCAGGGCG GAAAAAGATC AGATGGGGAA CAGGATGGAG CAGCTGGAAG TATGGATGCT 
TCTACCCAGG GCTTATTAGA AGGCATTGGG CTAGATGGTG ACACATTGGC TCCCATGGAG 
ACAGATGAAC CTACTGCTTC AGACTCTAAG GGCAAATCTA AAATCACACC AGCAATGGCT 
GCCAGAATTA AGCAAATCAA GCCTTTGTTA TCAGCTTCCT CCAGATTAGG CCGAGCACTT 
GCTGAGCTAT TTGGACTTCT TGTTAAACTT TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG 
AGCCATCATG CTGCCAGCAC CACTACAGCA CCGACACCTG CCGCGCGATC AACAGCCTCA 
GCTCTCACTA AGCTCTTGAC TAAGGGGTTA TCTTGGCAGC CCCCACCATA TACACCTACT 
CCCCGATTCA GGCTGACATT CTTCATCTGT TCAGTTGGTT TCACATCCCC AATGCTGTTT 
GATGAGAGGA AGTATCCCTA CCACCTCATG CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC 
AATGCTCTTT TTGAAACTTT CAACTGGGCT CTGTCCATGG GAGGTAAAGT TCCTGTTTCT 
GAGGGATTGG AACACTCAGA CTTGCCTGAT GGCACAGGAG AATTCCTAGA TGCCTGGCTT 
ATGCTGGTGG AGAAGATGGT GAATCCCACC ACGGTGCTTG AATCTCCACA TTCGCTGCCT 
GCCAAATTGC CTGGAGGTGT CCAGAACTTT CCCCAGTTCA GTGCACTGCG CTTCCTTGTG 
GTAACTCAGA AAGCAGCCTT TACTTGCATC AAAAACTTAT GGAACCGGAA ACCCCTGAAG 
GTATATGGTG GACGAATGGC TGAATCGATG CTGGCCATTC TATGCCACAT CCTCCGAGGA 
GAACCTGTGA TTCGAGAGAG ACTAAGCAAG GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT 
ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG GAACCTCAAG TCAACCAGCA ACAACTGCAA 
CAGCTCATGG ACATGGGCTT CACAAGGGAA CATGCAATGG AGGCACTGTT GAACACCAGC 
ACCATGGAGC AGGCCACAGA GTACCTTTTA ACCCACCCTC CTCCAATCAT GGGAGGAGTT 
GTTCGGGATC TCAGCATGTC TGAAGAGGAC CAGATGATGA GAGCAATTGC TATGTCTCTG 
GGACAGGATA TTCCAATGGA TCAAAGGGCA GAGTCACCTG AGGAAGTTGC TTGCCGGAAG 
GAGGAAGAGG AACGGAAAGC TCGGGAAAAG CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG 
AAGTTCCAGG ATGCTGACCC GTTGGAACAA GATGAGCTCC ACACTTTCAC AGATACTATG 
TTGCCAGGCT GCTTCCACCT TCTTGATGAG CTGCCAGACA CAGTATACCG TGTGTGTGAC 
CTGATCATGA CAGCAATCAA ACGTAATGGA GCAGATTATC GTGACATGAT TCTGAAGCAA 
GTAGTCAATC AGGTGTGGGA AGCTGCTGAT GTATTGATCA AAGCTGCTCT TCCCCTGACA 
ACAAGTGACA CAAAAACCGT GTCAGAGTGG ATAAGTCAGA TGGCCACACT GCCCCAGGCC 
TCCAATTTGG CTACTAGAAT CTTGCTTTTA ACGCTACTTT TTGAGGAGTT GAAGCTACCT 
TGTGCTTGGG TGGTTGAATC AAGTGGCATC CTTAATGTCC TAATCAAACT CTTGGAAGTG 
GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG CAGAAGGAAG TCCAGACCCC AAAGTGGATC 
ACACCAGTGT TGCTCCTGAT TGATTTCTAT GAAAAGACAG CCATCTCCTC AAAAAGGAGA 
GCCCAGATGA CTAAGTACCT GCAATCCAAC AGCAACAACT GGCGCTGGTT TGATGATCGC 
TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC AACAATAGCA CTATTGATTC TGCCTGGAAA 
TCTGGAGAGA CAAGCGTGCG ATTCACTGCA GGCCGAAGAA GATACACGGT CCAATTCACT 
ACAATGGTGC AGGTTAATGA GGAAACAGGG AACCGACGCC CTGTGATGCT GACTCTCCTC 
AGGGTACCTC GGCTGAATAA AAATTCAAAA AACAGCAATG GACAGGAACT AGAGAAGACG 
CTGGAAGAAA GCAAAGAAAT GGATATCAAA CGTAAAGAAA ATAAAGGCAA TGATACCCCT 
TTGGCCCTAG AGAGTACAAA CACTGAAAAG GAGACAAGCC TGGAGGAAAC AAAAATCGGG 
GAGATCCTGA TCCAGGGCTT GACAGAAGAT ATGGTGACTG TTTTAATCCG GGCCTGCGTG 
AGCATGCTGG GAGTCCCTGT GGACCCAGAT ACTTTGCATG CCACCCTTCG TCTCTGTCTG 
AGGCTCACCC GGGACCACAA ATATGCCATG ATGTTTGCAG AACTGAAGAG TACCCGCATG 
ATCTTGAATT TGACCCAGAG CTCAGGCTTC AATGGGTTTA CTCCCCTGGT CACCCTTCTC 
TTAAGACACA TCATTGAGGA CCCCTGTACC CTTCGTCATA CCATGGAAAA GGTTGTTCGC 
TCAGCAGCTA CAAGTGGAGC TGGTAGCACT ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC 
TCTCGGGAGA TCAACTACAT CCTTCGTGTC CTTGGGCCAG CCGCATGCCG CAATCCAGAC 
ATATTCACAG AAGTGGCCAA CTGCTGTATC CGCATCGCCC TTCCTGCCCC TCGAGGCTCA 
GGAACTGCTT CAGATGATGA ATTTGAGAAT CTTAGAATTA AAGGCCCTAA TGCTGTACAG 
CTGGTGAAGA CCACCCCTTT GAAGCCCTCA CCTCTGCCTG TCATCCCTGA TACTATCAAG 
GAAGTGATCT ATGATATGCT GAATGCTCTG GCTGCATACC ATGCTCCAGA GGAAGATAAA 
TCTGATCCTA AACCTGGGGT TATGACCCAA GAGGTTGGCC AGCTCCTGCA AGACATGGGT 
GATGATGTAT ACCAGCAGTA CCGGTCACTT ACGCGTCAGA GCAGTGACTT TGATACGCAG 
TCAGGTTTTT CCATTAATAG TCAGGTCTTT GCTGCAGATG GTGCCTCCAC TGAGACTTCC 
GCATCTGGGA CCTCCCAAGG AGAGGCTTCA ACTCCAGAGG AGTCTCGAGA TGGGAAGAAA 
GATAAAGAAG GGGACCGGGC CTCTGAGGAA GGCAAACAGA AAGGCAAGGG CAGCAAACCT 
TTAATGCCTA CCTCCACTAT CCTTCGTCTT CTGGCAGAGT TGGTGAGGTC CTATGTTGGT 
ATTGCTACCC TGATTGCCAA CTACAGCTAC ACTGTGGGCC AGTCTGAACT GATCAAAGAG 
GACTGCAGTG TGCTAGCTTT TGTTCTGGAC CACCTGCTCC CACATACCCA GAATGCAGAA 
GACAAGGACA CCCCTGCCTT GGCCCGCCTG TTCCTCGCAA GCCTGGCTGC TGCAGGGAGT 
GGCACAGATG CCCAGGTGGC CCTAGTGAAT GAAGTAAAAG CAGCCCTTGG ACGGGCACTG 
GCTATGGCTG AGAGTACAGA GAAACATGCC AGGCTTCAGG CAGTGATGTG TATCATCAGT 
ACTATCATGG AGTCCTGCCC CTCCACCTCC AGCTTCTACA GCAGTGCCAC AGCGAAGACC 
CAGCACAATG GCATGAACAA CATCATTCGG CTTTTCCTGA AGAAGGGACT GGTTAATGAC 
CTGGCCAGAG TACCTCACAG CTTAGACCTG TCCAGTCCCA ACATGGCCAA CACAGTCAAT 
GCTGCTCTGA AGCCTTTGGA AACACTTTCC CGGATTGTGA ACCAGCCCAG TAGCCTTTTT 
GGCAGCAAGA GTGCTTCTAG CAAGAACAAG TCTGAGCAGG ATGCCCAAGG AGCCTCTCAA 
GATTCCAGTA GCAACCAGCA GGACCCAGGC GAGCCTGGGG AAGCAGAAGT GCAGGAGGAG 
GATCATGATG TCACTCAGAC AGAGGTGGCA GATGGGGATA TCATGGATGG GGAGGCTGAA 
ACCGACTCAG TGGTGATTGC TGGGCAGCCT GAGGTGCTCA GTTCACAAGA GATGCAGGTT 
GAGAATGAGC TGGAGGACCT GATAGATGAG TTGCTTGAGA GGGATGGCGG ATCTGGGAAC 
AGTACAATTA TAGATTCCAT GAATATCCTG GACCCTGAGG ATGAGGAGGA GCACACTCAG 
GAAGAGGACA GCAGTGGCAG TAACGAGGAT GAGGATGATA GTCAGGATGA AGAGGAGGAG 
GAGGAGGAAG ATGAGGAAGA TGATCAGGAG GATGATGAAG GTGAAGAGGG AGATGAAGAC 
GATGACGACG ATGGCTCTGA GATGGAATTG GATGAGGATT ATCCTGATAT GAACGCTTCT 
CCCTTGGTCC GATTTGAGCG CTTTGACCGG GAGGATGATC TCATCATTGA GTTTGACAAC 
ATGTTCTCCA GTGCTACAGA CATCCCCCCA TCCCCAGGAA ATATCCCTAC CACCCATCCA 
CTGATGGTGC GCCATGCAGA CCACAGTTCT CTGACACTGG GCAGTGGCTC TTCAACAACT 
CGTCTCACCC AGGGCATCGG GCGCAGTCAG AGGACCCTAA GGCAGCTGAC GGCCAATACT 
GGCCACACCA TTCATGTTCA CTACCCTGGG AATCGCCAGC CCAACCCTCC TCTTATACTG 
CAGAGGTTGC TTGGTCCCTC AGCTGCTGCT GACATCCTTC AGCTGAGCAG CAGCCTTCCC 
CTACAAAGCC GGGGTCGGGC CCGCCTCCTG GTAGGCAACG ATGACGTCCA CATCATCGCC 
CGTTCTGATG ATGAGCTGCT GGATGACTTT TTCCATGATC AGAGCACAGC TACCAGCCAA 
GCAGGAACCC TGTCCAGCAT CCCCACAGCC CTGACCCGCT GGACAGAAGA ATGCAAAGTT 
CTCGATGCTG AGAGCATGCA TGACTGTGTT TCAGTGGTTA AAGTGTCCAT TGTCAATCAC 
CTGGAATTCC TGAGGGATGA GGAGCTGGAA GAAAGGCGAG AGAAGCGCAG GAAACAACTG 
GCTGAGGAAG AAACAAAGAT AACTGATAAA GGCAAAGAAG ATAAGGAGAA CAGGGATCAG 
AGTGCACAGT GTACTGCATC TAAGTCAAAT GACTCCACTG AACAGAATCT CTCAGATGGG 
ACGCCTATGC CTGACAGCTA CCCAACAACC CCATCTTCAA CTGATGCAGC TACATCTGAG 
TCCAAGGAGA CCCTTGGCAC TCTGCAATCC TCACAACAGC AACCAACACT CCCAACCCCA 
CCAGCTTTGG GAGAGGTTCC TCAGGAGCTG CAGTCTCCAG CTGGAGAAGG GGGCAGCTCT 
ACACAGCTAT TGATGCCTGT AGAGCCAGAG GAATTGGGTC CCACAAGGCC AAGTGGGGAA 
GCAGAAACAA CTCAGATGGA GTTATCCCCA GCTCCCACTA TAACCTCACT TTCCCCAGAG 
AGAGCTGAGG ATTCTGATGC ACTGACGGCT GTCAGCAGTC AGCTAGAAGG CTCTCCTATG 
GATACAAGCA GCCTGGCTTC CTGTACCTTA GAGGAGGCTG TGGGTGACAC TTCAGCAGCT 
GGCAGTTCTG AGCAGCCCAG AGCAGGCAGC TCCACTCCTG GGGATGCCCC ACCAGCTGTG 
GCGGAAGTGC AAGGCAGGAG TGATGGGTCA GGGGAATCTG CCCAGCCACC TGAGGACAGC 
TCCCCACCTG CATCCTCTGA GAGCTCTTCC ACCAGAGATT CTGCCGTGGC CATTTCTGGA 
GCAGATTCCC GAGGAATCCT AGAAGAGCCG TTGCCTTCAA CAAGCAGTGA AGAAGAAGAT 
CCCCTTGCGG GTATCAGTCT CCCTGAAGGT GTGGACCCCT CTTTTCTGGC TGCCCTGCCT 
GATGACATCC GTCGGGAAGT TCTACAGAAC CAGCTAGGCA TTCGTCCACC AACCCGGACT 
GCCCCCTCCA CAAATAGCTC AGCGCCTGCA GTGGTGGGGA ATCCTGGTGT GACTGAAGTG 
AGCCCTGAGT TTCTGGCTGC CCTGCCTCCA GCCATTCAGG AGGAAGTACT GGCACAGCAG 
AGAGCTGAGC AGCAGCGACG AGAACTAGCA CAGAATGCCA GCTCAGACAC CCCTATGGAC 
CCTGTGACCT TCATCCAGAC TCTGCCCTCA GACCTGCGCC GTAGTGTCCT AGAGGATATG 
GAGGACAGTG TGTTAGCTGT GATGCCACCT GACATTGCAG CTGAGGCTCA AGCCCTGAGA 
CGAGAGCAAG AAGCCCGGCA GCGACAGCTC ATGCATGAGC GTCTGTTTGG GCACAGTAGC 
ACCTCCGCAC TCTCTGCTAT TCTCCGAAGC CCGGCTTTCA CCAGTCGCTT AAGTGGCAAC 
CGTGGGGTCC AGTATACTCG CCTTGCTGTG CAGAGAGGTG GCACCTTCCA GATGGGGGGT 
AGCAGCAGCC ATAACAGGCC TTCTGGCAGT AATGTAGATA CTCTCCTCCG CCTCCGAGGA 
CGGCTCCTTC TGGACCACGA AGCCCTTTCT TGTCTCTTGG TCCTACTTTT TGTGGATGAG 
CCAAAGCTCA ATACTAGCCG TCTACACCGA GTACTGAGAA ATCTCTGCTA CCATGCCCAG 
ACCCGCCACT GGGTCATCCG CAGTCTGCTC TCCATCTTGC AGCGCAGCAG TGAGAGTGAG 
CTATGCATTG AAACACCCAA ACTCACTACA AGTGAGGAAA AGGGCAAAAA GTCGAGCAAG 
AGCTGTGGGT CAAGTAGCCA TGAGAACCGT CCCCTGGACC TGCTACACAA GATGGAGTCA 
AAGAGCTCCA ACCAGCTTTC CTGGCTCTCA GTATCCATGG ATGCAGCCCT AGGCTGCAGG 
ACTAATATAT TTCAGATCCA GCGTTCAGGG GGGCGTAAAC ATACCGAGAA GCATGCAAGC 
GGTGGCTCCA CCGTCCACAT CCATCCCCAA GCTGCTCCTG TTGTCTGCAG ACACGTTTTG 
GATACACTCA TTCAATTGGC CAAGGTATTT CCCAGCCACT TCACACAGCA GCGGACCAAA 
GAAACAAACT GTGAGAGTGA TCGGGAAAGG GGCAATAAGG CCTGTAGCCC ATGCTCCTCA 
CAGTCCTCCA GCAGTGGCAT TTGCACAGAC TTCTGGGACT TATTGGTAAA ACTGGACAAC 
ATGAATGTCA GCCGGAAAGG CAAGAACTCC GTGAAGTCAG TGCCAGTGAG CGCTGGCGGT 
GAGGGGGAAA CCTCTCCATA CAGCCTCGAG GCCTCTCCAC TGGGGCAGCT CATGAACATG 
TTGTCACACC CAGTCATCCG CCGGAGCTCT CTCTTAACTG AGAAACTCCT CAGACTCCTT 
TCTCTCATCT CAATTGCTCT CCCAGAAAAC AAGGTGTCAG AAGCACAGGC TAATTCTGGC 
AGCGGTGCTT CCTCCACCAC CACTGCCACC TCAACCACAT CTACCACCAC CACCACTGCC 
GCCTCCACCA CGCCCACACC CCCTACTGCA CCCACCCCTG TCACTTCTGC TCCAGCCCTG 
GTTGCTGCCA CGGCTATTTC CACCATTGTC GTAGCTGCTT CGACCACAGT GACTACCCCC 
ACGACTGCTA CCACTACTGT TTCAATTTCT CCCACTACTA AGGGCAGCAA ATCTCCAGCG 
AAGGTGAGTG ATGGGGGCAG CAGCAGTACA GACTTTAAGA TGGTGTCCTC TGGCCTCACT 
GAAAACCAGC TACAGCTCTC TGTAGAGGTG TTGACATCCC ACTCTTGTTC TGAGGAAGGC 
TTAGAGGATG CAGCCAACGT ACTACTGCAG CTCTCCCGGG GGGACTCTGG GACCCGGGAC 
ACTGTTCTCA AGCTGCTACT GAATGGAGCC CGCCATCTGG GTTATACCCT TTGTAAACAA 
ATAGGTACCC TGCTGGCCGA GCTGCGGGAA TACAACCTCG AGCAGCAGCG GCGAGCCCAA 
TGTGAAACCC TCTCTCCTGA TGGCCTGCCT GAGGAGCAGC CACAGACCAC CAAGCTGAAG 
GGCAAAATGC AGAGCAGGTT TGACATGGCT GAGAATGTGG TAATTGTGGC ATCTCAGAAG 
CGACCTTTGG GTGGCCGGGA GCTCCAGCTG CCTTCTATGT CCATGTTGAC ATCCAAGACA 
TCTACCCAGA AGTTCTTCTT GAGGGTACTA CAGGTCATCA TCCAGCTCCG GGACGACACG 
CGCCGGGCTA ACAAGAAAGC CAAGCAGACA GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA 
GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG CTGGAGGCTG AGGCTGATGC CATTATACAA 
ATGGTACGTG AGGGTCAAAG GGCGCGGAGA CAGCAACAAG CAGCAACGTC GGAGTCTAGC 
CAGTCAGAGG CGTCTGTCCG GAGGGAGGAA TCACCCATGG ATGTGGACCA GCCATCTCCC 
AGTGCTCAAG ATACTCAATC CATTGCCTCC GATGGAACCC CACAGGGGGA GAAGGAAAAG 
GAAGAAAGAC CACCTGAGTT ACCCCTGCTC AGCGAGCAGC TGAGTTTGGA CGAGCTGTGG 
GACATGCTTG GGGAGTGTCT AAAGGAACTA GAGGAATCCC ATGACCAGCA TGCGGTGCTA 
GTGCTACAGC CTGCTGTCGA GGCCTTCTTT CTGGTCCATG CCACAGAGCG GGAGAGCAAG 
CCTCCTGTCC GAGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCTCCTCCA 
CTCTCCCCTG CCCCCTTAAC CCCAGCCACG CCTTCCTCCC TTGACCCATT CTTCTCCCGG 
GAGCCCTCAT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ACACACAGAA GTTCCTTCGC 
TTTGCAGAGA CTCACCGCAC TGTGTTAAAC CAGATCCTAC GGCAGTCCAC GACCCACCTT 
GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT TGATGTCAAG 
CGCAAATATT TCCGCCAAGA GCTGGAGCGT TTAGATGAGG GGCTCCGGAA AGAAGACATG 
GCTGTGCATG TCCGTCGTGA CCATGTGTTT GAAGACTCCT ATCGTGAGCT GCATCGCAAA 
TCCCCCGAAG AAATGAAGAA TCGATTGTAT ATAGTATTTG AAGGAGAAGA AGGGCAGGAT 
GCTGGTGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GAGAGATGTT TAACCCTATG 
TATGCCTTGT TCCGTACCTC ACCTGGTGAT CGAGTCACCT ACACCATCAA TCCATCTTCC 
CACTGCAACC CCAACCACCT CAGCTACTTC AAGTTTGTCG GACGCATTGT GGCCAAAGCT 
GTATATGACA ACCGTCTTCT GGAGTGCTAC TTTACTCGAT CCTTTTACAA ACACATCTTG 
GGCAAGTCAG TCAGATATAC AGATATGGAG AGTGAAGATT ACCACTTCTA CCAAGGTCTG 
GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGCTATG ACCTCACCTT CAGCACTGAG 
GTCCAAGAGT TTGGAGTTTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC CAACATCTTG 
GTAACAGAGG AGAATAAGAA GGAGTATGTA CACCTGGTAT GCCAGATGAG AATGACAGGA 
GCCATCCGCA AGCAGTTGGC GGCTTTCTTA GAAGGCTTCT ATGAGATCAT TCCAAAGCGC 
CTCATTTCCA TCTTCACTGA GCAGGAGTTA GAGCTGCTTA TATCAGGACT GCCCACCATT 
GACATCGATG ATCTGAAATC CAACACTGAA TACCACAAGT ACCAGTCCAA CTCTATTCAG 
ATCCAGTGGT TCTGGAGAGC ATTGCGTTCT TTCGATCAAG CTGACCGTGC CAAGTTCCTC 
CAGTTTGTCA CAGGTACTTC CAAGGTACCC CTGCAAGGCT TTGCTGCCCT CGAAGGCATG 
AATGGCATTC AGAAGTTTCA GATCCATCGA GATGACAGGT CCACAGATCG CCTGCCTTCA 
GCTCACACAT GTTTTAATCA GCTGGATCTG CCTGCCTATG AGAGCTTTGA GAAGCTCCGC 
CACATGCTAC TGTTGGCTAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUWE1 ( XM_017029206.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884695.1 Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X19, mRNA.

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CloneID	OHu99122
Clone ID Related Accession (Same CDS sequence)	XM_017029206.1
Accession Version	XM_017029206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GTTGCAGATG TGGCTATGGA AAGCAGTCCA GGCTCATCCA TCTCTATGGA GCACAGGCTG 
GATGTTGAAT TAAGGGCATC AGGTTCCAGC AGCAGCACTA ACATCTCTTC TGGCCCCAGC 
CCTGGTCCCA GTCCCGGCCC CGGCACCGGC CCTGGCCCCG GCCCCGGCCC CGGCCCCGGC 
CCTGGCCCCG GCCCCGGCCC CGGTCCTGGT CCCGGCCCTG GCCCCGGCCC TGGCCCTGGC 
CCCCGTCCTG GAGTCCAGTG TATTCCACAA CGAGCAGCAC TTCTGAAATC CATGTTGAAT 
TTCCTCAAGA AGGCCATCCA AGACCCTGCT TTCTCAGATG GCATACGACA TGTGATGGAT 
GGTTCTCTGC CTACCTCCCT GAAACACATC ATCAGCAATG CAGAATACTA TGGCCCATCA 
CTCTTCCTCC TAGCTACTGA AGTGGTGACT GTGTTTGTAT TTCAAGAACC ATCACTGCTC 
TCCTCACTCC AGGACAATGG ATTGACAGAT GTCATGCTGC ATGCACTGCT TATCAAAGAT 
GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC CTCCCAAATG TATTCAGTGC ACTCTGTTTG 
AATGCCCGAG GTCTTCAGTC TTTTGTTCAG TGTCAGCCTT TTGAACGCCT CTTCAAAGTT 
CTTCTGTCTC CAGATTACCT CCCAGCCATG CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG 
GATACTGCAT CCAACCTGGG GAGTGCTGTC GATGAGCTCA TGAGACATCA GCCCACCCTT 
AAAACAGATG CAACGACTGC CATCATCAAG TTACTTGAAG AAATCTGTAA TCTTGGAAGG 
GACCCCAAAT ACATCTGTCA GAAGCCATCA ATCCAGAAGG CAGATGGCAC TGCCACTGCT 
CCTCCCCCAA GGTCTAATCA TGCCGCAGAA GAAGCCTCTA GTGAGGATGA GGAGGAAGAG 
GAAGTACAGG CCATGCAGAG CTTTAATTCT ACCCAGCAAA ATGAAACTGA GCCTAATCAG 
CAGGTTGTTG GTACAGAGGA ACGTATTCCT ATTCCCCTCA TGGATTACAT CCTTAATGTG 
ATGAAATTTG TGGAATCTAT TCTGAGCAAC AATACAACAG ATGACCACTG CCAGGAATTT 
GTGAATCAGA AAGGACTGTT GCCTTTGGTT ACCATTTTGG GTCTTCCCAA TCTGCCCATT 
GACTTTCCCA CATCTGCTGC CTGTCAGGCT GTTGCAGGTG TCTGCAAATC CATATTGACA 
CTGTCACATG AACCCAAAGT CCTTCAAGAG GGTCTCCTTC AGTTGGACTC CATCCTCTCC 
TCCCTGGAGC CCTTACACCG CCCCATTGAA TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA 
CTGGCTTGCG CAGGCAATGT TGCTGATGCT ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG 
CATGCACTCA CTGCTGCCCA TGCCTACATC ATGATGTTTG TTCATACTTG CAGAGTTGGA 
CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC CAGTGGGGCT CTCAATTGGG TCTGAGTGTT 
TTGAGCAAGC TGAGCCAGTT ATACTGTTCC CTGGTGTGGG AAAGCACTGT CCTCCTCTCT 
CTGTGTACCC CAAACAGCCT ACCATCTGGG TGTGAATTTG GCCAGGCAGA TATGCAGAAA 
CTGGTTCCAA AGGATGAGAA GGCAGGTACG ACCCAGGGCG GAAAAAGATC AGATGGGGAA 
CAGGATGGAG CAGCTGGAAG TATGGATGCT TCTACCCAGG GCTTATTAGA AGGCATTGGG 
CTAGATGGTG ACACATTGGC TCCCATGGAG ACAGATGAAC CTACTGCTTC AGACTCTAAG 
GGCAAATCTA AAATCACACC AGCAATGGCT GCCAGAATTA AGCAAATCAA GCCTTTGTTA 
TCAGCTTCCT CCAGATTAGG CCGAGCACTT GCTGAGCTAT TTGGACTTCT TGTTAAACTT 
TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG AGCCATCATG CTGCCAGCAC CACTACAGCA 
CCGACACCTG CCGCGCGATC AACAGCCTCA GCTCTCACTA AGCTCTTGAC TAAGGGGTTA 
TCTTGGCAGC CCCCACCATA TACACCTACT CCCCGATTCA GGCTGACATT CTTCATCTGT 
TCAGTTGGTT TCACATCCCC AATGCTGTTT GATGAGAGGA AGTATCCCTA CCACCTCATG 
CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC AATGCTCTTT TTGAAACTTT CAACTGGGCT 
CTGTCCATGG GAGGTAAAGT TCCTGTTTCT GAGGGATTGG AACACTCAGA CTTGCCTGAT 
GGCACAGGAG AATTCCTAGA TGCCTGGCTT ATGCTGGTGG AGAAGATGGT GAATCCCACC 
ACGGTGCTTG AATCTCCACA TTCGCTGCCT GCCAAATTGC CTGGAGGTGT CCAGAACTTT 
CCCCAGTTCA GTGCACTGCG CTTCCTTGTG GTAACTCAGA AAGCAGCCTT TACTTGCATC 
AAAAACTTAT GGAACCGGAA ACCCCTGAAG GTATATGGTG GACGAATGGC TGAATCGATG 
CTGGCCATTC TATGCCACAT CCTCCGAGGA GAACCTGTGA TTCGAGAGAG ACTAAGCAAG 
GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG 
GAACCTCAAG TCAACCAGCA ACAACTGCAA CAGCTCATGG ACATGGGCTT CACAAGGGAA 
CATGCAATGG AGGCACTGTT GAACACCAGC ACCATGGAGC AGGCCACAGA GTACCTTTTA 
ACCCACCCTC CTCCAATCAT GGGAGGAGTT GTTCGGGATC TCAGCATGTC TGAAGAGGAC 
CAGATGATGA GAGCAATTGC TATGTCTCTG GGACAGGATA TTCCAATGGA TCAAAGGGCA 
GAGTCACCTG AGGAAGTTGC TTGCCGGAAG GAGGAAGAGG AACGGAAAGC TCGGGAAAAG 
CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG AAGTTCCAGG ATGCTGACCC GTTGGAACAA 
GATGAGCTCC ACACTTTCAC AGATACTATG TTGCCAGGCT GCTTCCACCT TCTTGATGAG 
CTGCCAGACA CAGTATACCG TGTGTGTGAC CTGATCATGA CAGCAATCAA ACGTAATGGA 
GCAGATTATC GTGACATGAT TCTGAAGCAA GTAGTCAATC AGGTGTGGGA AGCTGCTGAT 
GTATTGATCA AAGCTGCTCT TCCCCTGACA ACAAGTGACA CAAAAACCGT GTCAGAGTGG 
ATAAGTCAGA TGGCCACACT GCCCCAGGCC TCCAATTTGG CTACTAGAAT CTTGCTTTTA 
ACGCTACTTT TTGAGGAGTT GAAGCTACCT TGTGCTTGGG TGGTTGAATC AAGTGGCATC 
CTTAATGTCC TAATCAAACT CTTGGAAGTG GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG 
CAGAAGGAAG TCCAGACCCC AAAGTGGATC ACACCAGTGT TGCTCCTGAT TGATTTCTAT 
GAAAAGACAG CCATCTCCTC AAAAAGGAGA GCCCAGATGA CTAAGTACCT GCAATCCAAC 
AGCAACAACT GGCGCTGGTT TGATGATCGC TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC 
AACAATAGCA CTATTGATTC TGCCTGGAAA TCTGGAGAGA CAAGCGTGCG ATTCACTGCA 
GGCCGAAGAA GATACACGGT CCAATTCACT ACAATGGTGC AGGTTAATGA GGAAACAGGG 
AACCGACGCC CTGTGATGCT GACTCTCCTC AGGGTACCTC GGCTGAATAA AAATTCAAAA 
AACAGCAATG GACAGGAACT AGAGAAGACG CTGGAAGAAA GCAAAGAAAT GGATATCAAA 
CGTAAAGAAA ATAAAGGCAA TGATACCCCT TTGGCCCTAG AGAGTACAAA CACTGAAAAG 
GAGACAAGCC TGGAGGAAAC AAAAATCGGG GAGATCCTGA TCCAGGGCTT GACAGAAGAT 
ATGGTGACTG TTTTAATCCG GGCCTGCGTG AGCATGCTGG GAGTCCCTGT GGACCCAGAT 
ACTTTGCATG CCACCCTTCG TCTCTGTCTG AGGCTCACCC GGGACCACAA ATATGCCATG 
ATGTTTGCAG AACTGAAGAG TACCCGCATG ATCTTGAATT TGACCCAGAG CTCAGGCTTC 
AATGGGTTTA CTCCCCTGGT CACCCTTCTC TTAAGACACA TCATTGAGGA CCCCTGTACC 
CTTCGTCATA CCATGGAAAA GGTTGTTCGC TCAGCAGCTA CAAGTGGAGC TGGTAGCACT 
ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC TCTCGGGAGA TCAACTACAT CCTTCGTGTC 
CTTGGGCCAG CCGCATGCCG CAATCCAGAC ATATTCACAG AAGTGGCCAA CTGCTGTATC 
CGCATCGCCC TTCCTGCCCC TCGAGGCTCA GGAACTGCTT CAGATGATGA ATTTGAGAAT 
CTTAGAATTA AAGGCCCTAA TGCTGTACAG CTGGTGAAGA CCACCCCTTT GAAGCCCTCA 
CCTCTGCCTG TCATCCCTGA TACTATCAAG GAAGTGATCT ATGATATGCT GAATGCTCTG 
GCTGCATACC ATGCTCCAGA GGAAGCAGAT AAATCTGATC CTAAACCTGG GGTTATGACC 
CAAGAGGTTG GCCAGCTCCT GCAAGACATG GGTGATGATG TATACCAGCA GTACCGGTCA 
CTTACGCGTC AGAGCAGTGA CTTTGATACG CAGTCAGGTT TTTCCATTAA TAGTCAGGTC 
TTTGCTGCAG ATGGTGCCTC CACTGAGACT TCCGCATCTG GGACCTCCCA AGGAGAGGCT 
TCAACTCCAG AGGAGTCTCG AGATGGGAAG AAAGATAAAG AAGGGGACCG GGCCTCTGAG 
GAAGGCAAAC AGAAAGGCAA GGGCAGCAAA CCTTTAATGC CTACCTCCAC TATCCTTCGT 
CTTCTGGCAG AGTTGGTGAG GTCCTATGTT GGTATTGCTA CCCTGATTGC CAACTACAGC 
TACACTGTGG GCCAGTCTGA ACTGATCAAA GAGGACTGCA GTGTGCTAGC TTTTGTTCTG 
GACCACCTGC TCCCACATAC CCAGAATGCA GAAGACAAGG ACACCCCTGC CTTGGCCCGC 
CTGTTCCTCG CAAGCCTGGC TGCTGCAGGG AGTGGCACAG ATGCCCAGGT GGCCCTAGTG 
AATGAAGTAA AAGCAGCCCT TGGACGGGCA CTGGCTATGG CTGAGAGTAC AGAGAAACAT 
GCCAGGCTTC AGGCAGTGAT GTGTATCATC AGTACTATCA TGGAGTCCTG CCCCTCCACC 
TCCAGCTTCT ACAGCAGTGC CACAGCGAAG ACCCAGCACA ATGGCATGAA CAACATCATT 
CGGCTTTTCC TGAAGAAGGG ACTGGTTAAT GACCTGGCCA GAGTACCTCA CAGCTTAGAC 
CTGTCCAGTC CCAACATGGC CAACACAGTC AATGCTGCTC TGAAGCCTTT GGAAACACTT 
TCCCGGATTG TGAACCAGCC CAGTAGCCTT TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC 
AAGTCTGAGC AGGATGCCCA AGGAGCCTCT CAAGATTCCA GTAGCAACCA GCAGGACCCA 
GGCGAGCCTG GGGAAGCAGA AGTGCAGGAG GAGGATCATG ATGTCACTCA GACAGAGGTG 
GCAGATGGGG ATATCATGGA TGGGGAGGCT GAAACCGACT CAGTGGTGAT TGCTGGGCAG 
CCTGAGGTGC TCAGTTCACA AGAGATGCAG GTTGAGAATG AGCTGGAGGA CCTGATAGAT 
GAGTTGCTTG AGAGGGATGG CGGATCTGGG AACAGTACAA TTATAGCAGA CATCCCCCCA 
TCCCCAGGAA ATATCCCTAC CACCCATCCA CTGATGGTGC GCCATGCAGA CCACAGTTCT 
CTGACACTGG GCAGTGGCTC TTCAACAACT CGTCTCACCC AGGGCATCGG GCGCAGTCAG 
AGGACCCTAA GGCAGCTGAC GGCCAATACT GGCCACACCA TTCATGTTCA CTACCCTGGG 
AATCGCCAGC CCAACCCTCC TCTTATACTG CAGAGGTTGC TTGGTCCCTC AGCTGCTGCT 
GACATCCTTC AGCTGAGCAG CAGCCTTCCC CTACAAAGCC GGGGTCGGGC CCGCCTCCTG 
GTAGGCAACG ATGACGTCCA CATCATCGCC CGTTCTGATG ATGAGCTGCT GGATGACTTT 
TTCCATGATC AGAGCACAGC TACCAGCCAA GCAGGAACCC TGTCCAGCAT CCCCACAGCC 
CTGACCCGCT GGACAGAAGA ATGCAAAGTT CTCGATGCTG AGAGCATGCA TGACTGTGTT 
TCAGTGGTTA AAGTGTCCAT TGTCAATCAC CTGGAATTCC TGAGGGATGA GGAGCTGGAA 
GAAAGGCGAG AGAAGCGCAG GAAACAACTG GCTGAGGAAG AAACAAAGAT AACTGATAAA 
GGCAAAGAAG ATAAGGAGAA CAGGGATCAG AGTGCACAGT GTACTGCATC TAAGTCAAAT 
GACTCCACTG AACAGAATCT CTCAGATGGG ACGCCTATGC CTGACAGCTA CCCAACAACC 
CCATCTTCAA CTGATGCAGC TACATCTGAG TCCAAGGAGA CCCTTGGCAC TCTGCAATCC 
TCACAACAGC AACCAACACT CCCAACCCCA CCAGCTTTGG GAGAGGTTCC TCAGGAGCTG 
CAGTCTCCAG CTGGAGAAGG GGGCAGCTCT ACACAGCTAT TGATGCCTGT AGAGCCAGAG 
GAATTGGGTC CCACAAGGCC AAGTGGGGAA GCAGAAACAA CTCAGATGGA GTTATCCCCA 
GCTCCCACTA TAACCTCACT TTCCCCAGAG AGAGCTGAGG ATTCTGATGC ACTGACGGCT 
GTCAGCAGTC AGCTAGAAGG CTCTCCTATG GATACAAGCA GCCTGGCTTC CTGTACCTTA 
GAGGAGGCTG TGGGTGACAC TTCAGCAGCT GGCAGTTCTG AGCAGCCCAG AGCAGGCAGC 
TCCACTCCTG GGGATGCCCC ACCAGCTGTG GCGGAAGTGC AAGGCAGGAG TGATGGGTCA 
GGGGAATCTG CCCAGCCACC TGAGGACAGC TCCCCACCTG CATCCTCTGA GAGCTCTTCC 
ACCAGAGATT CTGCCGTGGC CATTTCTGGA GCAGATTCCC GAGGAATCCT AGAAGAGCCG 
TTGCCTTCAA CAAGCAGTGA AGAAGAAGAT CCCCTTGCGG GTATCAGTCT CCCTGAAGGT 
GTGGACCCCT CTTTTCTGGC TGCCCTGCCT GATGACATCC GTCGGGAAGT TCTACAGAAC 
CAGCTAGGCA TTCGTCCACC AACCCGGACT GCCCCCTCCA CAAATAGCTC AGCGCCTGCA 
GTGGTGGGGA ATCCTGGTGT GACTGAAGTG AGCCCTGAGT TTCTGGCTGC CCTGCCTCCA 
GCCATTCAGG AGGAAGTACT GGCACAGCAG AGAGCTGAGC AGCAGCGACG AGAACTAGCA 
CAGAATGCCA GCTCAGACAC CCCTATGGAC CCTGTGACCT TCATCCAGAC TCTGCCCTCA 
GACCTGCGCC GTAGTGTCCT AGAGGATATG GAGGACAGTG TGTTAGCTGT GATGCCACCT 
GACATTGCAG CTGAGGCTCA AGCCCTGAGA CGAGAGCAAG AAGCCCGGCA GCGACAGCTC 
ATGCATGAGC GTCTGTTTGG GCACAGTAGC ACCTCCGCAC TCTCTGCTAT TCTCCGAAGC 
CCGGCTTTCA CCAGTCGCTT AAGTGGCAAC CGTGGGGTCC AGTATACTCG CCTTGCTGTG 
CAGAGAGGTG GCACCTTCCA GATGGGGGGT AGCAGCAGCC ATAACAGGCC TTCTGGCAGT 
AATGTAGATA CTCTCCTCCG CCTCCGAGGA CGGCTCCTTC TGGACCACGA AGCCCTTTCT 
TGTCTCTTGG TCCTACTTTT TGTGGATGAG CCAAAGCTCA ATACTAGCCG TCTACACCGA 
GTACTGAGAA ATCTCTGCTA CCATGCCCAG ACCCGCCACT GGGTCATCCG CAGTCTGCTC 
TCCATCTTGC AGCGCAGCAG TGAGAGTGAG CTATGCATTG AAACACCCAA ACTCACTACA 
AGTGAGGAAA AGGGCAAAAA GTCGAGCAAG AGCTGTGGGT CAAGTAGCCA TGAGAACCGT 
CCCCTGGACC TGCTACACAA GATGGAGTCA AAGAGCTCCA ACCAGCTTTC CTGGCTCTCA 
GTATCCATGG ATGCAGCCCT AGGCTGCAGG ACTAATATAT TTCAGATCCA GCGTTCAGGG 
GGGCGTAAAC ATACCGAGAA GCATGCAAGC GGTGGCTCCA CCGTCCACAT CCATCCCCAA 
GCTGCTCCTG TTGTCTGCAG ACACGTTTTG GATACACTCA TTCAATTGGC CAAGGTATTT 
CCCAGCCACT TCACACAGCA GCGGACCAAA GAAACAAACT GTGAGAGTGA TCGGGAAAGG 
GGCAATAAGG CCTGTAGCCC ATGCTCCTCA CAGTCCTCCA GCAGTGGCAT TTGCACAGAC 
TTCTGGGACT TATTGGTAAA ACTGGACAAC ATGAATGTCA GCCGGAAAGG CAAGAACTCC 
GTGAAGTCAG TGCCAGTGAG CGCTGGCGGT GAGGGGGAAA CCTCTCCATA CAGCCTCGAG 
GCCTCTCCAC TGGGGCAGCT CATGAACATG TTGTCACACC CAGTCATCCG CCGGAGCTCT 
CTCTTAACTG AGAAACTCCT CAGACTCCTT TCTCTCATCT CAATTGCTCT CCCAGAAAAC 
AAGGTGTCAG AAGCACAGGC TAATTCTGGC AGCGGTGCTT CCTCCACCAC CACTGCCACC 
TCAACCACAT CTACCACCAC CACCACTGCC GCCTCCACCA CGCCCACACC CCCTACTGCA 
CCCACCCCTG TCACTTCTGC TCCAGCCCTG GTTGCTGCCA CGGCTATTTC CACCATTGTC 
GTAGCTGCTT CGACCACAGT GACTACCCCC ACGACTGCTA CCACTACTGT TTCAATTTCT 
CCCACTACTA AGGGCAGCAA ATCTCCAGCG AAGGTGAGTG ATGGGGGCAG CAGCAGTACA 
GACTTTAAGA TGGTGTCCTC TGGCCTCACT GAAAACCAGC TACAGCTCTC TGTAGAGGTG 
TTGACATCCC ACTCTTGTTC TGAGGAAGGC TTAGAGGATG CAGCCAACGT ACTACTGCAG 
CTCTCCCGGG GGGACTCTGG GACCCGGGAC ACTGTTCTCA AGCTGCTACT GAATGGAGCC 
CGCCATCTGG GTTATACCCT TTGTAAACAA ATAGGTACCC TGCTGGCCGA GCTGCGGGAA 
TACAACCTCG AGCAGCAGCG GCGAGCCCAA TGTGAAACCC TCTCTCCTGA TGGCCTGCCT 
GAGGAGCAGC CACAGACCAC CAAGCTGAAG GGCAAAATGC AGAGCAGGTT TGACATGGCT 
GAGAATGTGG TAATTGTGGC ATCTCAGAAG CGACCTTTGG GTGGCCGGGA GCTCCAGCTG 
CCTTCTATGT CCATGTTGAC ATCCAAGACA TCTACCCAGA AGTTCTTCTT GAGGGTACTA 
CAGGTCATCA TCCAGCTCCG GGACGACACG CGCCGGGCTA ACAAGAAAGC CAAGCAGACA 
GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG 
CTGGAGGCTG AGGCTGATGC CATTATACAA ATGGTACGTG AGGGTCAAAG GGCGCGGAGA 
CAGCAACAAG CAGCAACGTC GGAGTCTAGC CAGTCAGAGG CGTCTGTCCG GAGGGAGGAA 
TCACCCATGG ATGTGGACCA GCCATCTCCC AGTGCTCAAG ATACTCAATC CATTGCCTCC 
GATGGAACCC CACAGGGGGA GAAGGAAAAG GAAGAAAGAC CACCTGAGTT ACCCCTGCTC 
AGCGAGCAGC TGAGTTTGGA CGAGCTGTGG GACATGCTTG GGGAGTGTCT AAAGGAACTA 
GAGGAATCCC ATGACCAGCA TGCGGTGCTA GTGCTACAGC CTGCTGTCGA GGCCTTCTTT 
CTGGTCCATG CCACAGAGCG GGAGAGCAAG CCTCCTGTCC GAGACACCCG TGAGAGCCAG 
CTGGCACACA TCAAGGACGA GCCTCCTCCA CTCTCCCCTG CCCCCTTAAC CCCAGCCACG 
CCTTCCTCCC TTGACCCATT CTTCTCCCGG GAGCCCTCAT CTATGCACAT CTCCTCAAGC 
CTGCCCCCTG ACACACAGAA GTTCCTTCGC TTTGCAGAGA CTCACCGCAC TGTGTTAAAC 
CAGATCCTAC GGCAGTCCAC GACCCACCTT GCTGATGGGC CTTTTGCTGT CCTGGTAGAC 
TACATTCGTG TCCTCGACTT TGATGTCAAG CGCAAATATT TCCGCCAAGA GCTGGAGCGT 
TTAGATGAGG GGCTCCGGAA AGAAGACATG GCTGTGCATG TCCGTCGTGA CCATGTGTTT 
GAAGACTCCT ATCGTGAGCT GCATCGCAAA TCCCCCGAAG AAATGAAGAA TCGATTGTAT 
ATAGTATTTG AAGGAGAAGA AGGGCAGGAT GCTGGTGGGC TCCTGCGGGA GTGGTATATG 
ATCATCTCTC GAGAGATGTT TAACCCTATG TATGCCTTGT TCCGTACCTC ACCTGGTGAT 
CGAGTCACCT ACACCATCAA TCCATCTTCC CACTGCAACC CCAACCACCT CAGCTACTTC 
AAGTTTGTCG GACGCATTGT GGCCAAAGCT GTATATGACA ACCGTCTTCT GGAGTGCTAC 
TTTACTCGAT CCTTTTACAA ACACATCTTG GGCAAGTCAG TCAGATATAC AGATATGGAG 
AGTGAAGATT ACCACTTCTA CCAAGGTCTG GTTTATCTGC TGGAAAATGA TGTCTCCACA 
CTAGGCTATG ACCTCACCTT CAGCACTGAG GTCCAAGAGT TTGGAGTTTG TGAAGTTCGT 
GACCTCAAAC CCAATGGGGC CAACATCTTG GTAACAGAGG AGAATAAGAA GGAGTATGTA 
CACCTGGTAT GCCAGATGAG AATGACAGGA GCCATCCGCA AGCAGTTGGC GGCTTTCTTA 
GAAGGCTTCT ATGAGATCAT TCCAAAGCGC CTCATTTCCA TCTTCACTGA GCAGGAGTTA 
GAGCTGCTTA TATCAGGACT GCCCACCATT GACATCGATG ATCTGAAATC CAACACTGAA 
TACCACAAGT ACCAGTCCAA CTCTATTCAG ATCCAGTGGT TCTGGAGAGC ATTGCGTTCT 
TTCGATCAAG CTGACCGTGC CAAGTTCCTC CAGTTTGTCA CAGGTACTTC CAAGGTACCC 
CTGCAAGGCT TTGCTGCCCT CGAAGGCATG AATGGCATTC AGAAGTTTCA GATCCATCGA 
GATGACAGGT CCACAGATCG CCTGCCTTCA GCTCACACAT GTTTTAATCA GCTGGATCTG 
CCTGCCTATG AGAGCTTTGA GAAGCTCCGC CACATGCTAC TGTTGGCTAT CCAGGAGTGC 
TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884695.1
CDS	3018..16121
Translation	MDPFISRREQKCMEIGTPRKNGVLVLRSSEAVYHLTTPFQIVCLHLPLSKSKPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVFVSPFAVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDVADVAMESSPGSSISMEHRLDVELRASGSSSSTNISSGPSPGPSPGPGTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPRPGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIADIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Target ORF information:

RefSeq Version	XM_017029206.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (HUWE1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029206.1

ORF Insert Sequence:

ATGGACCCAT TCATCAGTAG AAGAGAGCAG AAATGTATGG AAATAGGAAC CCCAAGAAAG 
AATGGAGTCC TAGTTTTACG AAGTTCGGAA GCTGTTTACC ATCTTACAAC ACCTTTCCAA 
ATAGTTTGTC TGCATCTACC TTTATCCAAA TCCAAGCCTG CAGACTGCAG AGCCTTAATA 
GACAAACTCA AAGTTTGTAA TGATGAGCAA CTTCTCTTGG AACTGCAGCA GATCAAAACA 
TGGAACATTG GAAAGTGCGA GTTATATCAC TGGGTGGACC TGTTGGACCG CTTCGATGGA 
ATACTGGCAG ATGCTGGACA GACAGTGGAG AATATGTCAT GGATGCTCGT ATGTGATAGG 
CCAGAAAGAG AGCAACTGAA AATGCTTCTC TTGGCTGTGT TGAACTTCAC AGCCTTGCTC 
ATTGAGTACA GCTTTTCCCG GCATCTGTAC AGTTCCATAG AGCATTTGAC AACTTTATTG 
GCTTCCTCTG ATATGCAAGT GGTGCTGGCA GTCCTCAATC TCCTATATGT ATTTAGCAAA 
AGATCAAACT ACATCACTCG TCTGGGATCT GACAAGAGGA CCCCGCTGCT AACTCGGCTA 
CAACATTTGG CAGAGAGCTG GGGTGGAAAG GAGAATGGCT TTGGACTTGC AGAATGTTGC 
AGAGACTTGC ATATGATGAA ATATCCACCC AGTGCAACTA CACTACACTT TGAATTCTAT 
GCAGATCCTG GGGCCGAGGT CAAAATTGAG AAAAGGACAA CTAGTAACAC ACTACATTAT 
ATTCACATAG AGCAACTTGA CAAGATTTCA GAAAGCCCTT CTGAAATCAT GGAATCTCTT 
ACCAAAATGT ACAGCATTCC TAAGGATAAG CAGATGCTGT TATTTACACA CATACGACTG 
GCCCATGGCT TTTCTAATCA CAGGAAGCGA TTGCAGGCAG TTCAGGCCAG ACTGCATGCA 
ATATCTATAT TAGTGTTTGT TTCTCCTTTT GCAGTGTATT CCAATGCCTT GCAGGAATCA 
GCAAACAGTA TCTTGTATAA TGGCTTGATA GAGGAGTTGG TAGATGTCCT TCAGATAACG 
GATAAGCAGC TTATGGAGAT TAAAGCAGCT TCTTTACGAA CATTAACATC AATTGTCCAC 
TTGGAGAGAA CTCCCAAACT CAGCAGTATT ATTGACTGTA CTGGAACTGC CTCCTACCAT 
GGATTTTTGC CAGTGCTTGT AAGGAACTGT ATCCAGGCCA TGATTGATCC TTCCATGGAT 
CCATACCCTC ACCAGTTTGC CACTGCTCTC TTCTCTTTTT TATACCATCT GGCCAGCTAC 
GATGCTGGTG GTGAAGCCTT GGTCTCCTGT GGAATGATGG AAGCCTTATT GAAGGTCATA 
AAGTTTCTTG GCGATGAACA GGACCAGATA ACATTTGTCA CCAGAGCCGT CAGAGTGGTT 
GACCTTATCA CCAACCTGGA TATGGCAGCT TTTCAATCCC ATAGTGGACT TTCTATCTTC 
ATTTATAGAC TTGAGCATGA AGTAGATTTG TGCCGAAAAG AATGTCCGTT TGTGATCAAG 
CCAAAGATCC AGAGACCCAA TACTACACAA GAAGGAGAGG AAATGGAAAC TGATATGGAT 
GTTGCAGATG TGGCTATGGA AAGCAGTCCA GGCTCATCCA TCTCTATGGA GCACAGGCTG 
GATGTTGAAT TAAGGGCATC AGGTTCCAGC AGCAGCACTA ACATCTCTTC TGGCCCCAGC 
CCTGGTCCCA GTCCCGGCCC CGGCACCGGC CCTGGCCCCG GCCCCGGCCC CGGCCCCGGC 
CCTGGCCCCG GCCCCGGCCC CGGTCCTGGT CCCGGCCCTG GCCCCGGCCC TGGCCCTGGC 
CCCCGTCCTG GAGTCCAGTG TATTCCACAA CGAGCAGCAC TTCTGAAATC CATGTTGAAT 
TTCCTCAAGA AGGCCATCCA AGACCCTGCT TTCTCAGATG GCATACGACA TGTGATGGAT 
GGTTCTCTGC CTACCTCCCT GAAACACATC ATCAGCAATG CAGAATACTA TGGCCCATCA 
CTCTTCCTCC TAGCTACTGA AGTGGTGACT GTGTTTGTAT TTCAAGAACC ATCACTGCTC 
TCCTCACTCC AGGACAATGG ATTGACAGAT GTCATGCTGC ATGCACTGCT TATCAAAGAT 
GTTCCTGCTA CCCGTGAAGT CCTTGGCTCC CTCCCAAATG TATTCAGTGC ACTCTGTTTG 
AATGCCCGAG GTCTTCAGTC TTTTGTTCAG TGTCAGCCTT TTGAACGCCT CTTCAAAGTT 
CTTCTGTCTC CAGATTACCT CCCAGCCATG CGGAGGAGGA GAAGTTCTGA TCCCCTTGGG 
GATACTGCAT CCAACCTGGG GAGTGCTGTC GATGAGCTCA TGAGACATCA GCCCACCCTT 
AAAACAGATG CAACGACTGC CATCATCAAG TTACTTGAAG AAATCTGTAA TCTTGGAAGG 
GACCCCAAAT ACATCTGTCA GAAGCCATCA ATCCAGAAGG CAGATGGCAC TGCCACTGCT 
CCTCCCCCAA GGTCTAATCA TGCCGCAGAA GAAGCCTCTA GTGAGGATGA GGAGGAAGAG 
GAAGTACAGG CCATGCAGAG CTTTAATTCT ACCCAGCAAA ATGAAACTGA GCCTAATCAG 
CAGGTTGTTG GTACAGAGGA ACGTATTCCT ATTCCCCTCA TGGATTACAT CCTTAATGTG 
ATGAAATTTG TGGAATCTAT TCTGAGCAAC AATACAACAG ATGACCACTG CCAGGAATTT 
GTGAATCAGA AAGGACTGTT GCCTTTGGTT ACCATTTTGG GTCTTCCCAA TCTGCCCATT 
GACTTTCCCA CATCTGCTGC CTGTCAGGCT GTTGCAGGTG TCTGCAAATC CATATTGACA 
CTGTCACATG AACCCAAAGT CCTTCAAGAG GGTCTCCTTC AGTTGGACTC CATCCTCTCC 
TCCCTGGAGC CCTTACACCG CCCCATTGAA TCCCCTGGGG GCTCAGTGTT GTTGCGAGAA 
CTGGCTTGCG CAGGCAATGT TGCTGATGCT ACCCTCTCAG CCCAGGCCAC ACCTCTGCTG 
CATGCACTCA CTGCTGCCCA TGCCTACATC ATGATGTTTG TTCATACTTG CAGAGTTGGA 
CAGAGTGAAA TTCGTTCCAT CTCCGTAAAC CAGTGGGGCT CTCAATTGGG TCTGAGTGTT 
TTGAGCAAGC TGAGCCAGTT ATACTGTTCC CTGGTGTGGG AAAGCACTGT CCTCCTCTCT 
CTGTGTACCC CAAACAGCCT ACCATCTGGG TGTGAATTTG GCCAGGCAGA TATGCAGAAA 
CTGGTTCCAA AGGATGAGAA GGCAGGTACG ACCCAGGGCG GAAAAAGATC AGATGGGGAA 
CAGGATGGAG CAGCTGGAAG TATGGATGCT TCTACCCAGG GCTTATTAGA AGGCATTGGG 
CTAGATGGTG ACACATTGGC TCCCATGGAG ACAGATGAAC CTACTGCTTC AGACTCTAAG 
GGCAAATCTA AAATCACACC AGCAATGGCT GCCAGAATTA AGCAAATCAA GCCTTTGTTA 
TCAGCTTCCT CCAGATTAGG CCGAGCACTT GCTGAGCTAT TTGGACTTCT TGTTAAACTT 
TGTGTGGGAT CTCCTGTCCG CCAGAGAAGG AGCCATCATG CTGCCAGCAC CACTACAGCA 
CCGACACCTG CCGCGCGATC AACAGCCTCA GCTCTCACTA AGCTCTTGAC TAAGGGGTTA 
TCTTGGCAGC CCCCACCATA TACACCTACT CCCCGATTCA GGCTGACATT CTTCATCTGT 
TCAGTTGGTT TCACATCCCC AATGCTGTTT GATGAGAGGA AGTATCCCTA CCACCTCATG 
CTGCAAAAAT TTCTCTGCTC CGGAGGCCAC AATGCTCTTT TTGAAACTTT CAACTGGGCT 
CTGTCCATGG GAGGTAAAGT TCCTGTTTCT GAGGGATTGG AACACTCAGA CTTGCCTGAT 
GGCACAGGAG AATTCCTAGA TGCCTGGCTT ATGCTGGTGG AGAAGATGGT GAATCCCACC 
ACGGTGCTTG AATCTCCACA TTCGCTGCCT GCCAAATTGC CTGGAGGTGT CCAGAACTTT 
CCCCAGTTCA GTGCACTGCG CTTCCTTGTG GTAACTCAGA AAGCAGCCTT TACTTGCATC 
AAAAACTTAT GGAACCGGAA ACCCCTGAAG GTATATGGTG GACGAATGGC TGAATCGATG 
CTGGCCATTC TATGCCACAT CCTCCGAGGA GAACCTGTGA TTCGAGAGAG ACTAAGCAAG 
GAGAAGGAGG GGTCTCGAGG AGAAGAGGAT ACAGGGCAAG AGGAAGGTGG CTCCCGCCGG 
GAACCTCAAG TCAACCAGCA ACAACTGCAA CAGCTCATGG ACATGGGCTT CACAAGGGAA 
CATGCAATGG AGGCACTGTT GAACACCAGC ACCATGGAGC AGGCCACAGA GTACCTTTTA 
ACCCACCCTC CTCCAATCAT GGGAGGAGTT GTTCGGGATC TCAGCATGTC TGAAGAGGAC 
CAGATGATGA GAGCAATTGC TATGTCTCTG GGACAGGATA TTCCAATGGA TCAAAGGGCA 
GAGTCACCTG AGGAAGTTGC TTGCCGGAAG GAGGAAGAGG AACGGAAAGC TCGGGAAAAG 
CAGGAGGAGG AAGAGGCTAA ATGTCTAGAG AAGTTCCAGG ATGCTGACCC GTTGGAACAA 
GATGAGCTCC ACACTTTCAC AGATACTATG TTGCCAGGCT GCTTCCACCT TCTTGATGAG 
CTGCCAGACA CAGTATACCG TGTGTGTGAC CTGATCATGA CAGCAATCAA ACGTAATGGA 
GCAGATTATC GTGACATGAT TCTGAAGCAA GTAGTCAATC AGGTGTGGGA AGCTGCTGAT 
GTATTGATCA AAGCTGCTCT TCCCCTGACA ACAAGTGACA CAAAAACCGT GTCAGAGTGG 
ATAAGTCAGA TGGCCACACT GCCCCAGGCC TCCAATTTGG CTACTAGAAT CTTGCTTTTA 
ACGCTACTTT TTGAGGAGTT GAAGCTACCT TGTGCTTGGG TGGTTGAATC AAGTGGCATC 
CTTAATGTCC TAATCAAACT CTTGGAAGTG GTTCAGCCCT GCCTCCAGGC AGCCAAGGAG 
CAGAAGGAAG TCCAGACCCC AAAGTGGATC ACACCAGTGT TGCTCCTGAT TGATTTCTAT 
GAAAAGACAG CCATCTCCTC AAAAAGGAGA GCCCAGATGA CTAAGTACCT GCAATCCAAC 
AGCAACAACT GGCGCTGGTT TGATGATCGC TCTGGGCGTT GGTGTAGTTA CAGTGCAAGC 
AACAATAGCA CTATTGATTC TGCCTGGAAA TCTGGAGAGA CAAGCGTGCG ATTCACTGCA 
GGCCGAAGAA GATACACGGT CCAATTCACT ACAATGGTGC AGGTTAATGA GGAAACAGGG 
AACCGACGCC CTGTGATGCT GACTCTCCTC AGGGTACCTC GGCTGAATAA AAATTCAAAA 
AACAGCAATG GACAGGAACT AGAGAAGACG CTGGAAGAAA GCAAAGAAAT GGATATCAAA 
CGTAAAGAAA ATAAAGGCAA TGATACCCCT TTGGCCCTAG AGAGTACAAA CACTGAAAAG 
GAGACAAGCC TGGAGGAAAC AAAAATCGGG GAGATCCTGA TCCAGGGCTT GACAGAAGAT 
ATGGTGACTG TTTTAATCCG GGCCTGCGTG AGCATGCTGG GAGTCCCTGT GGACCCAGAT 
ACTTTGCATG CCACCCTTCG TCTCTGTCTG AGGCTCACCC GGGACCACAA ATATGCCATG 
ATGTTTGCAG AACTGAAGAG TACCCGCATG ATCTTGAATT TGACCCAGAG CTCAGGCTTC 
AATGGGTTTA CTCCCCTGGT CACCCTTCTC TTAAGACACA TCATTGAGGA CCCCTGTACC 
CTTCGTCATA CCATGGAAAA GGTTGTTCGC TCAGCAGCTA CAAGTGGAGC TGGTAGCACT 
ACCTCTGGTG TTGTGTCTGG CAGCCTCGGC TCTCGGGAGA TCAACTACAT CCTTCGTGTC 
CTTGGGCCAG CCGCATGCCG CAATCCAGAC ATATTCACAG AAGTGGCCAA CTGCTGTATC 
CGCATCGCCC TTCCTGCCCC TCGAGGCTCA GGAACTGCTT CAGATGATGA ATTTGAGAAT 
CTTAGAATTA AAGGCCCTAA TGCTGTACAG CTGGTGAAGA CCACCCCTTT GAAGCCCTCA 
CCTCTGCCTG TCATCCCTGA TACTATCAAG GAAGTGATCT ATGATATGCT GAATGCTCTG 
GCTGCATACC ATGCTCCAGA GGAAGCAGAT AAATCTGATC CTAAACCTGG GGTTATGACC 
CAAGAGGTTG GCCAGCTCCT GCAAGACATG GGTGATGATG TATACCAGCA GTACCGGTCA 
CTTACGCGTC AGAGCAGTGA CTTTGATACG CAGTCAGGTT TTTCCATTAA TAGTCAGGTC 
TTTGCTGCAG ATGGTGCCTC CACTGAGACT TCCGCATCTG GGACCTCCCA AGGAGAGGCT 
TCAACTCCAG AGGAGTCTCG AGATGGGAAG AAAGATAAAG AAGGGGACCG GGCCTCTGAG 
GAAGGCAAAC AGAAAGGCAA GGGCAGCAAA CCTTTAATGC CTACCTCCAC TATCCTTCGT 
CTTCTGGCAG AGTTGGTGAG GTCCTATGTT GGTATTGCTA CCCTGATTGC CAACTACAGC 
TACACTGTGG GCCAGTCTGA ACTGATCAAA GAGGACTGCA GTGTGCTAGC TTTTGTTCTG 
GACCACCTGC TCCCACATAC CCAGAATGCA GAAGACAAGG ACACCCCTGC CTTGGCCCGC 
CTGTTCCTCG CAAGCCTGGC TGCTGCAGGG AGTGGCACAG ATGCCCAGGT GGCCCTAGTG 
AATGAAGTAA AAGCAGCCCT TGGACGGGCA CTGGCTATGG CTGAGAGTAC AGAGAAACAT 
GCCAGGCTTC AGGCAGTGAT GTGTATCATC AGTACTATCA TGGAGTCCTG CCCCTCCACC 
TCCAGCTTCT ACAGCAGTGC CACAGCGAAG ACCCAGCACA ATGGCATGAA CAACATCATT 
CGGCTTTTCC TGAAGAAGGG ACTGGTTAAT GACCTGGCCA GAGTACCTCA CAGCTTAGAC 
CTGTCCAGTC CCAACATGGC CAACACAGTC AATGCTGCTC TGAAGCCTTT GGAAACACTT 
TCCCGGATTG TGAACCAGCC CAGTAGCCTT TTTGGCAGCA AGAGTGCTTC TAGCAAGAAC 
AAGTCTGAGC AGGATGCCCA AGGAGCCTCT CAAGATTCCA GTAGCAACCA GCAGGACCCA 
GGCGAGCCTG GGGAAGCAGA AGTGCAGGAG GAGGATCATG ATGTCACTCA GACAGAGGTG 
GCAGATGGGG ATATCATGGA TGGGGAGGCT GAAACCGACT CAGTGGTGAT TGCTGGGCAG 
CCTGAGGTGC TCAGTTCACA AGAGATGCAG GTTGAGAATG AGCTGGAGGA CCTGATAGAT 
GAGTTGCTTG AGAGGGATGG CGGATCTGGG AACAGTACAA TTATAGCAGA CATCCCCCCA 
TCCCCAGGAA ATATCCCTAC CACCCATCCA CTGATGGTGC GCCATGCAGA CCACAGTTCT 
CTGACACTGG GCAGTGGCTC TTCAACAACT CGTCTCACCC AGGGCATCGG GCGCAGTCAG 
AGGACCCTAA GGCAGCTGAC GGCCAATACT GGCCACACCA TTCATGTTCA CTACCCTGGG 
AATCGCCAGC CCAACCCTCC TCTTATACTG CAGAGGTTGC TTGGTCCCTC AGCTGCTGCT 
GACATCCTTC AGCTGAGCAG CAGCCTTCCC CTACAAAGCC GGGGTCGGGC CCGCCTCCTG 
GTAGGCAACG ATGACGTCCA CATCATCGCC CGTTCTGATG ATGAGCTGCT GGATGACTTT 
TTCCATGATC AGAGCACAGC TACCAGCCAA GCAGGAACCC TGTCCAGCAT CCCCACAGCC 
CTGACCCGCT GGACAGAAGA ATGCAAAGTT CTCGATGCTG AGAGCATGCA TGACTGTGTT 
TCAGTGGTTA AAGTGTCCAT TGTCAATCAC CTGGAATTCC TGAGGGATGA GGAGCTGGAA 
GAAAGGCGAG AGAAGCGCAG GAAACAACTG GCTGAGGAAG AAACAAAGAT AACTGATAAA 
GGCAAAGAAG ATAAGGAGAA CAGGGATCAG AGTGCACAGT GTACTGCATC TAAGTCAAAT 
GACTCCACTG AACAGAATCT CTCAGATGGG ACGCCTATGC CTGACAGCTA CCCAACAACC 
CCATCTTCAA CTGATGCAGC TACATCTGAG TCCAAGGAGA CCCTTGGCAC TCTGCAATCC 
TCACAACAGC AACCAACACT CCCAACCCCA CCAGCTTTGG GAGAGGTTCC TCAGGAGCTG 
CAGTCTCCAG CTGGAGAAGG GGGCAGCTCT ACACAGCTAT TGATGCCTGT AGAGCCAGAG 
GAATTGGGTC CCACAAGGCC AAGTGGGGAA GCAGAAACAA CTCAGATGGA GTTATCCCCA 
GCTCCCACTA TAACCTCACT TTCCCCAGAG AGAGCTGAGG ATTCTGATGC ACTGACGGCT 
GTCAGCAGTC AGCTAGAAGG CTCTCCTATG GATACAAGCA GCCTGGCTTC CTGTACCTTA 
GAGGAGGCTG TGGGTGACAC TTCAGCAGCT GGCAGTTCTG AGCAGCCCAG AGCAGGCAGC 
TCCACTCCTG GGGATGCCCC ACCAGCTGTG GCGGAAGTGC AAGGCAGGAG TGATGGGTCA 
GGGGAATCTG CCCAGCCACC TGAGGACAGC TCCCCACCTG CATCCTCTGA GAGCTCTTCC 
ACCAGAGATT CTGCCGTGGC CATTTCTGGA GCAGATTCCC GAGGAATCCT AGAAGAGCCG 
TTGCCTTCAA CAAGCAGTGA AGAAGAAGAT CCCCTTGCGG GTATCAGTCT CCCTGAAGGT 
GTGGACCCCT CTTTTCTGGC TGCCCTGCCT GATGACATCC GTCGGGAAGT TCTACAGAAC 
CAGCTAGGCA TTCGTCCACC AACCCGGACT GCCCCCTCCA CAAATAGCTC AGCGCCTGCA 
GTGGTGGGGA ATCCTGGTGT GACTGAAGTG AGCCCTGAGT TTCTGGCTGC CCTGCCTCCA 
GCCATTCAGG AGGAAGTACT GGCACAGCAG AGAGCTGAGC AGCAGCGACG AGAACTAGCA 
CAGAATGCCA GCTCAGACAC CCCTATGGAC CCTGTGACCT TCATCCAGAC TCTGCCCTCA 
GACCTGCGCC GTAGTGTCCT AGAGGATATG GAGGACAGTG TGTTAGCTGT GATGCCACCT 
GACATTGCAG CTGAGGCTCA AGCCCTGAGA CGAGAGCAAG AAGCCCGGCA GCGACAGCTC 
ATGCATGAGC GTCTGTTTGG GCACAGTAGC ACCTCCGCAC TCTCTGCTAT TCTCCGAAGC 
CCGGCTTTCA CCAGTCGCTT AAGTGGCAAC CGTGGGGTCC AGTATACTCG CCTTGCTGTG 
CAGAGAGGTG GCACCTTCCA GATGGGGGGT AGCAGCAGCC ATAACAGGCC TTCTGGCAGT 
AATGTAGATA CTCTCCTCCG CCTCCGAGGA CGGCTCCTTC TGGACCACGA AGCCCTTTCT 
TGTCTCTTGG TCCTACTTTT TGTGGATGAG CCAAAGCTCA ATACTAGCCG TCTACACCGA 
GTACTGAGAA ATCTCTGCTA CCATGCCCAG ACCCGCCACT GGGTCATCCG CAGTCTGCTC 
TCCATCTTGC AGCGCAGCAG TGAGAGTGAG CTATGCATTG AAACACCCAA ACTCACTACA 
AGTGAGGAAA AGGGCAAAAA GTCGAGCAAG AGCTGTGGGT CAAGTAGCCA TGAGAACCGT 
CCCCTGGACC TGCTACACAA GATGGAGTCA AAGAGCTCCA ACCAGCTTTC CTGGCTCTCA 
GTATCCATGG ATGCAGCCCT AGGCTGCAGG ACTAATATAT TTCAGATCCA GCGTTCAGGG 
GGGCGTAAAC ATACCGAGAA GCATGCAAGC GGTGGCTCCA CCGTCCACAT CCATCCCCAA 
GCTGCTCCTG TTGTCTGCAG ACACGTTTTG GATACACTCA TTCAATTGGC CAAGGTATTT 
CCCAGCCACT TCACACAGCA GCGGACCAAA GAAACAAACT GTGAGAGTGA TCGGGAAAGG 
GGCAATAAGG CCTGTAGCCC ATGCTCCTCA CAGTCCTCCA GCAGTGGCAT TTGCACAGAC 
TTCTGGGACT TATTGGTAAA ACTGGACAAC ATGAATGTCA GCCGGAAAGG CAAGAACTCC 
GTGAAGTCAG TGCCAGTGAG CGCTGGCGGT GAGGGGGAAA CCTCTCCATA CAGCCTCGAG 
GCCTCTCCAC TGGGGCAGCT CATGAACATG TTGTCACACC CAGTCATCCG CCGGAGCTCT 
CTCTTAACTG AGAAACTCCT CAGACTCCTT TCTCTCATCT CAATTGCTCT CCCAGAAAAC 
AAGGTGTCAG AAGCACAGGC TAATTCTGGC AGCGGTGCTT CCTCCACCAC CACTGCCACC 
TCAACCACAT CTACCACCAC CACCACTGCC GCCTCCACCA CGCCCACACC CCCTACTGCA 
CCCACCCCTG TCACTTCTGC TCCAGCCCTG GTTGCTGCCA CGGCTATTTC CACCATTGTC 
GTAGCTGCTT CGACCACAGT GACTACCCCC ACGACTGCTA CCACTACTGT TTCAATTTCT 
CCCACTACTA AGGGCAGCAA ATCTCCAGCG AAGGTGAGTG ATGGGGGCAG CAGCAGTACA 
GACTTTAAGA TGGTGTCCTC TGGCCTCACT GAAAACCAGC TACAGCTCTC TGTAGAGGTG 
TTGACATCCC ACTCTTGTTC TGAGGAAGGC TTAGAGGATG CAGCCAACGT ACTACTGCAG 
CTCTCCCGGG GGGACTCTGG GACCCGGGAC ACTGTTCTCA AGCTGCTACT GAATGGAGCC 
CGCCATCTGG GTTATACCCT TTGTAAACAA ATAGGTACCC TGCTGGCCGA GCTGCGGGAA 
TACAACCTCG AGCAGCAGCG GCGAGCCCAA TGTGAAACCC TCTCTCCTGA TGGCCTGCCT 
GAGGAGCAGC CACAGACCAC CAAGCTGAAG GGCAAAATGC AGAGCAGGTT TGACATGGCT 
GAGAATGTGG TAATTGTGGC ATCTCAGAAG CGACCTTTGG GTGGCCGGGA GCTCCAGCTG 
CCTTCTATGT CCATGTTGAC ATCCAAGACA TCTACCCAGA AGTTCTTCTT GAGGGTACTA 
CAGGTCATCA TCCAGCTCCG GGACGACACG CGCCGGGCTA ACAAGAAAGC CAAGCAGACA 
GGCAGGCTAG GTTCCTCCGG TTTAGGCTCA GCTAGCAGCA TCCAGGCAGC TGTTCGGCAG 
CTGGAGGCTG AGGCTGATGC CATTATACAA ATGGTACGTG AGGGTCAAAG GGCGCGGAGA 
CAGCAACAAG CAGCAACGTC GGAGTCTAGC CAGTCAGAGG CGTCTGTCCG GAGGGAGGAA 
TCACCCATGG ATGTGGACCA GCCATCTCCC AGTGCTCAAG ATACTCAATC CATTGCCTCC 
GATGGAACCC CACAGGGGGA GAAGGAAAAG GAAGAAAGAC CACCTGAGTT ACCCCTGCTC 
AGCGAGCAGC TGAGTTTGGA CGAGCTGTGG GACATGCTTG GGGAGTGTCT AAAGGAACTA 
GAGGAATCCC ATGACCAGCA TGCGGTGCTA GTGCTACAGC CTGCTGTCGA GGCCTTCTTT 
CTGGTCCATG CCACAGAGCG GGAGAGCAAG CCTCCTGTCC GAGACACCCG TGAGAGCCAG 
CTGGCACACA TCAAGGACGA GCCTCCTCCA CTCTCCCCTG CCCCCTTAAC CCCAGCCACG 
CCTTCCTCCC TTGACCCATT CTTCTCCCGG GAGCCCTCAT CTATGCACAT CTCCTCAAGC 
CTGCCCCCTG ACACACAGAA GTTCCTTCGC TTTGCAGAGA CTCACCGCAC TGTGTTAAAC 
CAGATCCTAC GGCAGTCCAC GACCCACCTT GCTGATGGGC CTTTTGCTGT CCTGGTAGAC 
TACATTCGTG TCCTCGACTT TGATGTCAAG CGCAAATATT TCCGCCAAGA GCTGGAGCGT 
TTAGATGAGG GGCTCCGGAA AGAAGACATG GCTGTGCATG TCCGTCGTGA CCATGTGTTT 
GAAGACTCCT ATCGTGAGCT GCATCGCAAA TCCCCCGAAG AAATGAAGAA TCGATTGTAT 
ATAGTATTTG AAGGAGAAGA AGGGCAGGAT GCTGGTGGGC TCCTGCGGGA GTGGTATATG 
ATCATCTCTC GAGAGATGTT TAACCCTATG TATGCCTTGT TCCGTACCTC ACCTGGTGAT 
CGAGTCACCT ACACCATCAA TCCATCTTCC CACTGCAACC CCAACCACCT CAGCTACTTC 
AAGTTTGTCG GACGCATTGT GGCCAAAGCT GTATATGACA ACCGTCTTCT GGAGTGCTAC 
TTTACTCGAT CCTTTTACAA ACACATCTTG GGCAAGTCAG TCAGATATAC AGATATGGAG 
AGTGAAGATT ACCACTTCTA CCAAGGTCTG GTTTATCTGC TGGAAAATGA TGTCTCCACA 
CTAGGCTATG ACCTCACCTT CAGCACTGAG GTCCAAGAGT TTGGAGTTTG TGAAGTTCGT 
GACCTCAAAC CCAATGGGGC CAACATCTTG GTAACAGAGG AGAATAAGAA GGAGTATGTA 
CACCTGGTAT GCCAGATGAG AATGACAGGA GCCATCCGCA AGCAGTTGGC GGCTTTCTTA 
GAAGGCTTCT ATGAGATCAT TCCAAAGCGC CTCATTTCCA TCTTCACTGA GCAGGAGTTA 
GAGCTGCTTA TATCAGGACT GCCCACCATT GACATCGATG ATCTGAAATC CAACACTGAA 
TACCACAAGT ACCAGTCCAA CTCTATTCAG ATCCAGTGGT TCTGGAGAGC ATTGCGTTCT 
TTCGATCAAG CTGACCGTGC CAAGTTCCTC CAGTTTGTCA CAGGTACTTC CAAGGTACCC 
CTGCAAGGCT TTGCTGCCCT CGAAGGCATG AATGGCATTC AGAAGTTTCA GATCCATCGA 
GATGACAGGT CCACAGATCG CCTGCCTTCA GCTCACACAT GTTTTAATCA GCTGGATCTG 
CCTGCCTATG AGAGCTTTGA GAAGCTCCGC CACATGCTAC TGTTGGCTAT CCAGGAGTGC 
TCTGAAGGCT TTGGGCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HUWE1
Entrez Gene ID	10075
Full Name	HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
Synonyms	ARF-BP1,HECTH9,HSPC272,Ib772,LASU1,MULE,URE-B1,UREB1
General protein information	Preferred Names HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase HUWE1 ARF-binding protein 1 BJ-HCC-24 tumor antigen HECT domain protein LASU1 HECT-type E3 ubiquitin transferase HUWE1 Mcl-1 ubiquitin ligase E3 URE-binding protein 1 homologous to E6AP carboxyl terminus homologous protein 9 large structure of UREB1 upstream regulatory element-binding protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.22
Summary	This gene encodes a protein containing a C-terminal HECT (E6AP type E3 ubiquitin protein ligase) domain that functions as an E3 ubiquitin ligase. The encoded protein is required for the ubiquitination and subsequent degradation of the anti-apoptotic protein Mcl1 (myeloid cell leukemia sequence 1 (BCL2-related)). This protein also ubiquitinates the p53 tumor suppressor, core histones, and DNA polymerase beta. Mutations in this gene are associated with Turner type X-linked syndromic cognitive disability. [provided by RefSeq, Aug 2013].
Disorder MIM:	300697
Disorder Html:	Mental retardation, X-linked syndromic, Turner type, 300706 (3)

Danio rerio (zebrafish)	huwe1	XP_005162338.1
Drosophila melanogaster (fruit fly)	CG8184	NP_573059.1
Pan troglodytes (chimpanzee)	HUWE1	XP_003317522.2
Xenopus tropicalis (tropical clawed frog)	huwe1	XP_004914249.1
	HUWE1	XP_001088987.1
Mus musculus (house mouse)	Huwe1	NP_067498.4
Canis lupus familiaris (dog)	HUWE1	XP_005641438.1
Bos taurus (cattle)	HUWE1	NP_001103474.1
Homo sapiens (human)	HUWE1	NP_113584.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HUWE1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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