PRP8 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PRP8
Entrez Gene ID	856570
Full Name	U4/U6-U5 snRNP complex subunit PRP8
Synonyms	DBF3,DNA39,RNA8,SLT21,USA2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179296.1	NP_012035.1	U4/U6-U5 snRNP complex subunit PRP8

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prpf8	NP_001178519.1
Neurospora crassa	NCU07832	XP_962953.1
Arabidopsis thaliana (thale cress)	SUS2	NP_178124.2
	PRPF8	XP_001117328.1
Canis lupus familiaris (dog)	PRPF8	XP_854384.1
Drosophila melanogaster (fruit fly)	Prp8	NP_610735.1
Caenorhabditis elegans (roundworm)	prp-8	NP_498785.1
Schizosaccharomyces pombe (fission yeast)	spp42	NP_593861.1
Magnaporthe oryzae (rice blast fungus)	MGG_05244	XP_003712798.1
Oryza sativa (rice)	Os06g0167000	NP_001056917.1
Homo sapiens (human)	PRPF8	NP_006436.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006885	XP_308873.2
Kluyveromyces lactis	KLLA0A05280g	XP_451233.1
Eremothecium gossypii	AGOS_ABR207W	NP_983156.2
Bos taurus (cattle)	PRPF8	NP_001099108.1
Mus musculus (house mouse)	Prpf8	NP_619600.2
Arabidopsis thaliana (thale cress)	AT4G38780	NP_195589.2
Xenopus tropicalis (tropical clawed frog)	prpf8	XP_002936105.1
Gallus gallus (chicken)	PRPF8	XP_415805.2
Danio rerio (zebrafish)	prpf8	NP_957270.2
Saccharomyces cerevisiae (baker's yeast)	PRP8	NP_012035.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Upon step 1 catalysis, Cwc25 contacts with the branch-site region, and enhanced crosslinks of Prp8 and Prp45 with nucleotides surrounding the branch-site were observed.
Title: Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes.

The Prp8 suppressor partially corrected both the early assembly and later activation defects of prp28-1, supporting a role for this U5 snRNP protein in both the ATP-independent and ATP-dependent functions of Prp28.
Title: An unanticipated early function of DEAD-box ATPase Prp28 during commitment to splicing is modulated by U5 snRNP protein Prp8.

We propose that the U5 snRNA IL1 is a platform for protein binding and is required for , Brr2 and Snu114 association with the U5 snRNA to form the U5 snRNP.
Title: The U5 snRNA internal loop 1 is a platform for Brr2, Snu114 and Prp8 protein binding during U5 snRNP assembly.

Together, these data establish that PRP8 is a metalloprotein that promotes exon ligation within the spliceosome.
Title: A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation.

Results report the structure of the Saccharomyces cerevisiae tri-snRNP, and show that U4/U6 snRNP forms a domain termed the arm, while a separate head domain adjacent to the arm harbors Brr2, and Prp8 and the GTPase Snu114 are located centrally.
Title: Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy.

The following PRP8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRP8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05362	NM_001179296.1 Latest version!	Saccharomyces cerevisiae S288C U4/U6-U5 snRNP complex subunit PRP8 (PRP8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRP8 ( NM_001179296.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012035.1 Saccharomyces cerevisiae S288C U4/U6-U5 snRNP complex subunit PRP8 (PRP8), partial mRNA.

Price & Availability↑

CloneID	OSi05362
Clone ID Related Accession (Same CDS sequence)	NM_001179296.1
Accession Version	NM_001179296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	U4/U6-U5 snRNP complex subunit PRP8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGTGGAC TACCGCCCCC ACCTCCTGGT TTTGAAGAGG ACAGCGACTT AGCACTTCCA 
CCACCACCAC CACCACCGCC TGGATACGAA ATCGAAGAAC TGGATAATCC GATGGTGCCA 
TCATCGGTAA ATGAGGATAC ATTCCTTCCG CCTCCACCAC CTCCTCCAAG CAACTTCGAA 
ATAAACGCTG AAGAAATTGT GGACTTCACA TTACCACCGC CACCACCCCC TCCAGGTCTA 
GATGAATTAG AGACTAAAGC AGAAAAGAAA GTAGAATTAC ATGGAAAGAG AAAATTGGAC 
ATAGGAAAAG ATACTTTCGT CACTCGAAAA TCAAGAAAGC GTGCAAAAAA AATGACCAAA 
AAGGCAAAGA GAAGCAATTT ATATACTCCT AAAGCCGAGA TGCCACCGGA ACATCTGCGA 
AAGATTATTA ATACTCACAG TGACATGGCC TCCAAAATGT ACAATACTGA TAAAAAGGCT 
TTTCTAGGCG CTCTAAAGTA TTTACCGCAC GCGATTTTAA AATTGTTAGA GAACATGCCT 
CATCCATGGG AGCAAGCCAA AGAAGTTAAA GTTTTATATC ACACTTCAGG AGCCATAACA 
TTTGTGAATG AAACACCAAG AGTAATAGAG CCTGTATATA CTGCCCAATG GTCAGCTACT 
TGGATAGCAA TGAGGAGAGA AAAACGAGAT AGAACACATT TTAAAAGAAT GAGATTTCCA 
CCTTTCGATG ATGACGAACC ACCTCTGTCA TATGAACAGC ACATAGAAAA TATCGAGCCA 
CTGGATCCTA TAAATCTTCC CTTAGACTCT CAAGATGATG AATATGTTAA AGATTGGCTC 
TACGATTCCC GGCCCTTGGA GGAGGATAGT AAAAAGGTCA ATGGTACATC TTATAAAAAG 
TGGAGTTTTG ATTTACCTGA AATGTCGAAC TTATATAGGT TATCAACGCC ACTTAGAGAT 
GAAGTAACTG ACAAAAATTA CTATTATCTT TTCGATAAAA AATCTTTTTT TAACGGTAAA 
GCCCTTAATA ACGCAATCCC TGGAGGTCCT AAATTTGAAC CTTTATACCC AAGAGAAGAG 
GAGGAAGATT ATAACGAATT CAACTCTATA GATAGAGTAA TATTTAGGGT TCCTATTAGA 
AGCGAATATA AGGTAGCGTT TCCTCATCTA TATAATTCCC GGCCCCGTTC AGTGCGCATA 
CCATGGTATA ATAATCCAGT GTCGTGTATT ATCCAGAACG ATGAGGAGTA CGACACGCCT 
GCACTTTTTT TTGATCCATC GCTGAACCCA ATACCTCACT TTATTGATAA TAATTCATCC 
CTCAACGTAT CAAATACAAA GGAAAACGGT GATTTCACTT TACCTGAAGA TTTTGCACCT 
CTTTTGGCTG AAGAAGAAGA ACTTATTTTG CCAAATACAA AAGATGCGAT GTCGTTATAT 
CATTCACCTT TTCCATTTAA TAGAACTAAA GGAAAAATGG TGAGAGCACA AGATGTTGCC 
CTGGCTAAAA AATGGTTTTT GCAACATCCT GACGAGGAGT ATCCCGTTAA AGTTAAGGTC 
TCTTACCAAA AGCTCTTGAA GAACTACGTG CTAAATGAAT TGCACCCTAC TTTGCCAACA 
AACCACAACA AAACTAAGTT ATTGAAGAGT TTAAAAAATA CCAAGTATTT TCAACAAACT 
ACAATTGATT GGGTAGAGGC AGGACTCCAG CTATGTCGAC AAGGACACAA TATGTTAAAT 
TTATTAATCC ACAGAAAGGG CCTAACTTAT TTACATCTCG ACTACAATTT CAATTTAAAA 
CCAACTAAAA CCTTAACCAC AAAAGAACGT AAAAAGTCAA GACTTGGAAA CTCATTTCAT 
TTGATGCGTG AATTGCTAAA AATGATGAAA CTGATTGTAG ATACACATGT GCAGTTTAGG 
TTAGGAAATG TAGATGCCTT CCAGTTAGCA GATGGTATAC ATTATATTTT GAACCACATA 
GGACAATTGA CCGGTATTTA TCGTTATAAA TATAAAGTAA TGCATCAAAT CCGCGCATGT 
AAAGATCTGA AACATATCAT ATATTATAAG TTTAATAAAA ATTTGGGAAA AGGACCTGGT 
TGTGGATTTT GGCAACCTGC GTGGAGAGTG TGGCTAAATT TCTTAAGAGG CACTATACCA 
CTACTCGAAA GATATATTGG AAACCTTATC ACACGTCAAT TTGAAGGCCG TTCTAATGAA 
ATTGTCAAAA CTACAACTAA ACAAAGACTT GACGCCTACT ACGATCTTGA GTTACGAAAC 
TCGGTCATGG ATGACATTTT AGAAATGATG CCTGAGAGTA TTCGACAGAA AAAGGCAAGA 
ACTATACTGC AACATTTAAG TGAGGCTTGG AGATGTTGGA AGGCTAATAT TCCATGGGAT 
GTTCCTGGTA TGCCAGCCCC AATCAAAAAA ATAATTGAAC GGTATATAAA ATCCAAAGCT 
GATGCCTGGG TATCTGCAGC TCATTACAAT CGAGAACGTA TAAAGCGTGG AGCTCACGTC 
GAAAAGACTA TGGTTAAAAA AAATCTGGGG CGTTTAACGA GACTTTGGAT CAAAAATGAG 
CAGGAACGTC AAAGGCAAAT ACAGAAAAAC GGTCCCGAAA TTACTCCCGA AGAGGCTACG 
ACCATATTTT CTGTTATGGT TGAGTGGCTT GAATCAAGGA GCTTTTCACC AATACCATTT 
CCACCTTTAA CTTACAAAAA TGATACTAAG ATATTAGTTC TTGCCCTCGA AGACCTAAAA 
GATGTCTACG CATCAAAAGT ACGTTTAAAT GCATCTGAAA GGGAAGAACT TGCGTTGATA 
GAGGAGGCTT ATGATAATCC TCACGATACT TTGAACAGAA TCAAAAAGTA CTTGTTGACC 
CAGCGAGTTT TTAAGCCTGT TGATATAACC ATGATGGAAA ACTATCAAAA CATTTCCCCT 
GTTTATTCAG TTGATCCCTT GGAAAAGATT ACCGATGCAT ATCTTGATCA GTATTTATGG 
TACGAAGCTG ACCAACGAAA GCTTTTCCCA AACTGGATTA AACCAAGTGA TTCAGAGATA 
CCACCTCTTC TGGTATACAA GTGGACTCAG GGTATAAACA ACCTATCTGA AATTTGGGAT 
GTATCCAGAG GCCAATCTGC AGTTTTGCTT GAGACTACTT TGGGTGAAAT GGCCGAAAAA 
ATTGACTTTA CTTTACTTAA TAGATTACTT CGCCTGATTG TAGATCCTAA TATTGCTGAC 
TATATCACTG CAAAAAATAA TGTTGTTATC AACTTTAAAG ATATGAGTCA CGTCAACAAA 
TATGGCTTAA TACGCGGGTT GAAGTTCGCT TCTTTCATAT TCCAATATTA CGGACTAGTT 
ATAGATCTTT TACTATTGGG TCAGGAAAGG GCTACAGATT TGGCTGGTCC AGCTAACAAT 
CCAAATGAAT TTATGCAATT CAAGAGCAAA GAAGTAGAAA AGGCACATCC GATCAGACTT 
TACACCAGAT ATTTAGATCG TATATATATG CTTTTTCACT TTGAAGAAGA TGAGGGAGAG 
GAATTGACTG ATGAATACTT GGCAGAGAAT CCAGATCCAA ACTTTGAAAA CAGCATCGGA 
TACAATAATA GAAAATGCTG GCCCAAAGAT TCGCGCATGA GGCTAATACG TCAGGACGTT 
AACCTAGGGC GAGCAGTGTT TTGGGAAATT CAAAGTAGGG TGCCAACTTC GTTAACGTCA 
ATTAAATGGG AAAATGCTTT TGTTTCCGTA TACAGTAAAA ACAATCCGAA CTTACTCTTC 
TCTATGTGTG GTTTTGAAGT CAGAATATTA CCAAGACAAA GAATGGAAGA GGTGGTTTCT 
AACGATGAAG GTGTTTGGGA TTTAGTTGAC GAAAGAACCA AGCAAAGAAC TGCAAAAGCA 
TATTTAAAGG TATCTGAAGA AGAAATCAAA AAATTTGACA GCAGAATAAG AGGCATATTG 
ATGGCCTCCG GTTCAACCAC TTTTACTAAA GTGGCCGCTA AATGGAACAC TTCACTGATA 
TCCCTTTTCA CATACTTTAG AGAAGCCATT GTTGCCACGG AGCCGTTATT AGATATCCTG 
GTGAAAGGAG AAACAAGAAT TCAAAACCGT GTCAAACTTG GTTTAAATTC GAAAATGCCA 
ACAAGATTTC CACCTGCTGT TTTTTATACA CCAAAAGAAC TTGGTGGGTT GGGTATGATT 
AGTGCTTCTC ATATTTTAAT TCCTGCATCT GACTTAAGTT GGTCAAAACA GACCGATACT 
GGAATTACGC ATTTTCGTGC TGGTATGACG CACGAGGATG AAAAATTAAT CCCAACTATC 
TTCCGTTACA TCACTACTTG GGAAAATGAA TTTTTAGACT CTCAAAGGGT GTGGGCCGAG 
TATGCAACGA AAAGGCAGGA AGCTATTCAG CAGAATAGGA GGTTAGCTTT TGAAGAACTT 
GAAGGATCGT GGGATCGTGG TATACCTCGT ATTAGCACTT TATTTCAAAG AGATCGACAT 
ACTCTAGCGT ATGACAGAGG TCACAGAATC CGTAGGGAAT TTAAACAGTA CTCTCTAGAG 
AGAAACAGCC CCTTTTGGTG GACAAACTCC CACCATGACG GTAAATTATG GAATTTAAAT 
GCCTATAGAA CTGATGTGAT TCAAGCCCTA GGAGGTATTG AAACAATTTT GGAACACACT 
TTATTTAAGG GGACTGGTTT TAATTCATGG GAAGGTTTGT TTTGGGAAAA GGCGTCCGGG 
TTTGAGGATT CCATGCAATT TAAGAAGCTA ACTCATGCTC AAAGAACTGG TTTAAGCCAA 
ATTCCAAATC GTAGATTTAC CTTATGGTGG TCACCAACTA TTAACAGAGC AAATGTCTAT 
GTCGGTTTCT TGGTGCAATT GGATCTGACT GGTATCTTCC TCCATGGTAA AATTCCTACG 
TTAAAAATAT CTTTAATTCA AATATTCCGT GCTCATTTGT GGCAAAAAAT ACATGAAAGT 
ATAGTTTTTG ATATTTGTCA AATTCTCGAT GGTGAGCTAG ATGTTTTGCA AATTGAATCT 
GTGACTAAAG AAACAGTGCA TCCCCGCAAA TCGTACAAGA TGAATTCATC TGCCGCTGAC 
ATAACTATGG AAAGTGTTCA TGAATGGGAA GTTTCAAAAC CCTCCCTATT ACATGAAACA 
AATGACTCAT TTAAGGGATT GATTACCAAC AAAATGTGGT TTGATGTACA ATTGAGATAC 
GGTGATTATG ATTCTCATGA TATATCTCGT TATGTGAGGG CAAAATTCCT CGATTATACA 
ACAGATAATG TCAGCATGTA TCCCTCCCCA ACAGGTGTTA TGATCGGTAT CGATTTGGCT 
TATAACATGT ATGATGCCTA TGGTAATTGG TTCAACGGAT TAAAACCTTT GATACAAAAT 
AGTATGAGAA CAATTATGAA AGCTAACCCA GCATTATATG TCCTTCGTGA ACGTATTAGA 
AAAGGTCTTC AAATATATCA ATCTAGTGTA CAGGAACCAT TTTTAAACTC CTCAAACTAT 
GCCGAGTTAT TCAATAACGA CATTAAACTC TTCGTTGACG ACACTAATGT GTATAGAGTT 
ACTGTCCACA AGACTTTTGA AGGGAACGTT GCTACAAAAG CAATTAATGG TTGCATTTTC 
ACTTTAAATC CAAAGACTGG GCATCTGTTT CTGAAAATTA TCCATACCTC TGTATGGGCC 
GGTCAAAAAC GTTTAAGCCA ATTGGCCAAA TGGAAAACAG CTGAGGAAGT TAGTGCTCTT 
GTGAGATCTT TGCCCAAAGA AGAGCAGCCA AAACAAATTA TCGTCACAAG AAAGGCAATG 
CTTGATCCTT TAGAGGTTCA CATGCTTGAC TTTCCTAACA TAGCAATCAG GCCAACAGAG 
CTGCGACTAC CATTTTCAGC TGCAATGTCA ATAGATAAAC TTTCTGATGT TGTGATGAAA 
GCTACTGAAC CTCAGATGGT TCTGTTTAAC ATTTATGATG ATTGGTTAGA CCGTATTTCA 
TCGTACACTG CATTTTCTAG ACTTACACTA CTTTTAAGAG CTTTAAAGAC GAACGAGGAA 
AGTGCCAAAA TGATTCTACT CAGTGACCCC ACGATCACTA TCAAATCGTA CCATTTATGG 
CCTTCATTCA CTGATGAACA ATGGATCACT ATCGAATCTC AAATGAGAGA TTTGATTTTG 
ACTGAGTACG GCAGAAAATA CAATGTCAAT ATATCTGCCT TAACTCAAAC GGAAATTAAA 
GATATTATAT TGGGTCAAAA TATCAAGGCA CCATCTGTGA AGAGACAAAA AATGGCTGAA 
TTAGAAGCTG CCAGGTCAGA AAAGCAGAAC GACGAAGAAG CTGCAGGTGC ATCAACTGTT 
ATGAAAACTA AAACTATCAA TGCGCAAGGT GAGGAGATAG TTGTAGTAGC TTCCGCTGAT 
TACGAAAGTC AAACGTTTAG TTCAAAGAAT GAATGGAGAA AATCTGCAAT TGCTAATACA 
CTATTATATT TGAGGTTAAA AAACATTTAT GTTTCGGCTG ATGATTTCGT GGAGGAACAG 
AATGTTTACG TCCTTCCAAA AAATTTATTA AAGAAGTTTA TTGAGATATC CGATGTGAAA 
ATCCAGGTGG CGGCCTTTAT TTATGGTATG TCAGCAAAAG ATCATCCGAA GGTGAAAGAA 
ATCAAGACTG TTGTGCTGGT ACCTCAATTA GGCCACGTAG GATCCGTTCA GATAAGCAAC 
ATCCCTGACA TCGGAGACCT TCCGGATACT GAAGGCCTTG AACTACTCGG TTGGATCCAT 
ACCCAGACAG AAGAACTTAA ATTCATGGCT GCGTCTGAAG TAGCAACTCA TTCTAAACTT 
TTTGCGGATA AGAAAAGAGA CTGCATTGAT ATTTCAATTT TTTCCACCCC GGGATCAGTA 
TCATTAAGCG CTTATAACCT GACCGACGAA GGTTACCAAT GGGGTGAAGA AAATAAGGAT 
ATAATGAACG TGCTATCGGA AGGGTTTGAG CCTACTTTCA GCACGCATGC CCAATTACTT 
CTCTCTGACC GTATCACAGG TAATTTTATA ATTCCATCTG GAAACGTTTG GAACTACACA 
TTTATGGGAA CAGCATTTAA CCAAGAAGGT GACTACAATT TTAAATACGG CATTCCTTTG 
GAATTCTATA ATGAGATGCA TCGTCCAGTA CACTTTTTAC AGTTTAGCGA ATTGGCGGGG 
GACGAAGAGT TAGAGGCCGA ACAAATCGAT GTATTTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012035.1
CDS	1..7242
Translation	MSGLPPPPPGFEEDSDLALPPPPPPPPGYEIEELDNPMVPSSVNEDTFLPPPPPPPSNFEINAEEIVDFTLPPPPPPPGLDELETKAEKKVELHGKRKLDIGKDTFVTRKSRKRAKKMTKKAKRSNLYTPKAEMPPEHLRKIINTHSDMASKMYNTDKKAFLGALKYLPHAILKLLENMPHPWEQAKEVKVLYHTSGAITFVNETPRVIEPVYTAQWSATWIAMRREKRDRTHFKRMRFPPFDDDEPPLSYEQHIENIEPLDPINLPLDSQDDEYVKDWLYDSRPLEEDSKKVNGTSYKKWSFDLPEMSNLYRLSTPLRDEVTDKNYYYLFDKKSFFNGKALNNAIPGGPKFEPLYPREEEEDYNEFNSIDRVIFRVPIRSEYKVAFPHLYNSRPRSVRIPWYNNPVSCIIQNDEEYDTPALFFDPSLNPIPHFIDNNSSLNVSNTKENGDFTLPEDFAPLLAEEEELILPNTKDAMSLYHSPFPFNRTKGKMVRAQDVALAKKWFLQHPDEEYPVKVKVSYQKLLKNYVLNELHPTLPTNHNKTKLLKSLKNTKYFQQTTIDWVEAGLQLCRQGHNMLNLLIHRKGLTYLHLDYNFNLKPTKTLTTKERKKSRLGNSFHLMRELLKMMKLIVDTHVQFRLGNVDAFQLADGIHYILNHIGQLTGIYRYKYKVMHQIRACKDLKHIIYYKFNKNLGKGPGCGFWQPAWRVWLNFLRGTIPLLERYIGNLITRQFEGRSNEIVKTTTKQRLDAYYDLELRNSVMDDILEMMPESIRQKKARTILQHLSEAWRCWKANIPWDVPGMPAPIKKIIERYIKSKADAWVSAAHYNRERIKRGAHVEKTMVKKNLGRLTRLWIKNEQERQRQIQKNGPEITPEEATTIFSVMVEWLESRSFSPIPFPPLTYKNDTKILVLALEDLKDVYASKVRLNASEREELALIEEAYDNPHDTLNRIKKYLLTQRVFKPVDITMMENYQNISPVYSVDPLEKITDAYLDQYLWYEADQRKLFPNWIKPSDSEIPPLLVYKWTQGINNLSEIWDVSRGQSAVLLETTLGEMAEKIDFTLLNRLLRLIVDPNIADYITAKNNVVINFKDMSHVNKYGLIRGLKFASFIFQYYGLVIDLLLLGQERATDLAGPANNPNEFMQFKSKEVEKAHPIRLYTRYLDRIYMLFHFEEDEGEELTDEYLAENPDPNFENSIGYNNRKCWPKDSRMRLIRQDVNLGRAVFWEIQSRVPTSLTSIKWENAFVSVYSKNNPNLLFSMCGFEVRILPRQRMEEVVSNDEGVWDLVDERTKQRTAKAYLKVSEEEIKKFDSRIRGILMASGSTTFTKVAAKWNTSLISLFTYFREAIVATEPLLDILVKGETRIQNRVKLGLNSKMPTRFPPAVFYTPKELGGLGMISASHILIPASDLSWSKQTDTGITHFRAGMTHEDEKLIPTIFRYITTWENEFLDSQRVWAEYATKRQEAIQQNRRLAFEELEGSWDRGIPRISTLFQRDRHTLAYDRGHRIRREFKQYSLERNSPFWWTNSHHDGKLWNLNAYRTDVIQALGGIETILEHTLFKGTGFNSWEGLFWEKASGFEDSMQFKKLTHAQRTGLSQIPNRRFTLWWSPTINRANVYVGFLVQLDLTGIFLHGKIPTLKISLIQIFRAHLWQKIHESIVFDICQILDGELDVLQIESVTKETVHPRKSYKMNSSAADITMESVHEWEVSKPSLLHETNDSFKGLITNKMWFDVQLRYGDYDSHDISRYVRAKFLDYTTDNVSMYPSPTGVMIGIDLAYNMYDAYGNWFNGLKPLIQNSMRTIMKANPALYVLRERIRKGLQIYQSSVQEPFLNSSNYAELFNNDIKLFVDDTNVYRVTVHKTFEGNVATKAINGCIFTLNPKTGHLFLKIIHTSVWAGQKRLSQLAKWKTAEEVSALVRSLPKEEQPKQIIVTRKAMLDPLEVHMLDFPNIAIRPTELRLPFSAAMSIDKLSDVVMKATEPQMVLFNIYDDWLDRISSYTAFSRLTLLLRALKTNEESAKMILLSDPTITIKSYHLWPSFTDEQWITIESQMRDLILTEYGRKYNVNISALTQTEIKDIILGQNIKAPSVKRQKMAELEAARSEKQNDEEAAGASTVMKTKTINAQGEEIVVVASADYESQTFSSKNEWRKSAIANTLLYLRLKNIYVSADDFVEEQNVYVLPKNLLKKFIEISDVKIQVAAFIYGMSAKDHPKVKEIKTVVLVPQLGHVGSVQISNIPDIGDLPDTEGLELLGWIHTQTEELKFMAASEVATHSKLFADKKRDCIDISIFSTPGSVSLSAYNLTDEGYQWGEENKDIMNVLSEGFEPTFSTHAQLLLSDRITGNFIIPSGNVWNYTFMGTAFNQEGDYNFKYGIPLEFYNEMHRPVHFLQFSELAGDEELEAEQIDVFS

Target ORF information:

RefSeq Version	NM_001179296.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C U4/U6-U5 snRNP complex subunit PRP8 (PRP8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179296.1

ORF Insert Sequence:

ATGAGTGGAC TACCGCCCCC ACCTCCTGGT TTTGAAGAGG ACAGCGACTT AGCACTTCCA 
CCACCACCAC CACCACCGCC TGGATACGAA ATCGAAGAAC TGGATAATCC GATGGTGCCA 
TCATCGGTAA ATGAGGATAC ATTCCTTCCG CCTCCACCAC CTCCTCCAAG CAACTTCGAA 
ATAAACGCTG AAGAAATTGT GGACTTCACA TTACCACCGC CACCACCCCC TCCAGGTCTA 
GATGAATTAG AGACTAAAGC AGAAAAGAAA GTAGAATTAC ATGGAAAGAG AAAATTGGAC 
ATAGGAAAAG ATACTTTCGT CACTCGAAAA TCAAGAAAGC GTGCAAAAAA AATGACCAAA 
AAGGCAAAGA GAAGCAATTT ATATACTCCT AAAGCCGAGA TGCCACCGGA ACATCTGCGA 
AAGATTATTA ATACTCACAG TGACATGGCC TCCAAAATGT ACAATACTGA TAAAAAGGCT 
TTTCTAGGCG CTCTAAAGTA TTTACCGCAC GCGATTTTAA AATTGTTAGA GAACATGCCT 
CATCCATGGG AGCAAGCCAA AGAAGTTAAA GTTTTATATC ACACTTCAGG AGCCATAACA 
TTTGTGAATG AAACACCAAG AGTAATAGAG CCTGTATATA CTGCCCAATG GTCAGCTACT 
TGGATAGCAA TGAGGAGAGA AAAACGAGAT AGAACACATT TTAAAAGAAT GAGATTTCCA 
CCTTTCGATG ATGACGAACC ACCTCTGTCA TATGAACAGC ACATAGAAAA TATCGAGCCA 
CTGGATCCTA TAAATCTTCC CTTAGACTCT CAAGATGATG AATATGTTAA AGATTGGCTC 
TACGATTCCC GGCCCTTGGA GGAGGATAGT AAAAAGGTCA ATGGTACATC TTATAAAAAG 
TGGAGTTTTG ATTTACCTGA AATGTCGAAC TTATATAGGT TATCAACGCC ACTTAGAGAT 
GAAGTAACTG ACAAAAATTA CTATTATCTT TTCGATAAAA AATCTTTTTT TAACGGTAAA 
GCCCTTAATA ACGCAATCCC TGGAGGTCCT AAATTTGAAC CTTTATACCC AAGAGAAGAG 
GAGGAAGATT ATAACGAATT CAACTCTATA GATAGAGTAA TATTTAGGGT TCCTATTAGA 
AGCGAATATA AGGTAGCGTT TCCTCATCTA TATAATTCCC GGCCCCGTTC AGTGCGCATA 
CCATGGTATA ATAATCCAGT GTCGTGTATT ATCCAGAACG ATGAGGAGTA CGACACGCCT 
GCACTTTTTT TTGATCCATC GCTGAACCCA ATACCTCACT TTATTGATAA TAATTCATCC 
CTCAACGTAT CAAATACAAA GGAAAACGGT GATTTCACTT TACCTGAAGA TTTTGCACCT 
CTTTTGGCTG AAGAAGAAGA ACTTATTTTG CCAAATACAA AAGATGCGAT GTCGTTATAT 
CATTCACCTT TTCCATTTAA TAGAACTAAA GGAAAAATGG TGAGAGCACA AGATGTTGCC 
CTGGCTAAAA AATGGTTTTT GCAACATCCT GACGAGGAGT ATCCCGTTAA AGTTAAGGTC 
TCTTACCAAA AGCTCTTGAA GAACTACGTG CTAAATGAAT TGCACCCTAC TTTGCCAACA 
AACCACAACA AAACTAAGTT ATTGAAGAGT TTAAAAAATA CCAAGTATTT TCAACAAACT 
ACAATTGATT GGGTAGAGGC AGGACTCCAG CTATGTCGAC AAGGACACAA TATGTTAAAT 
TTATTAATCC ACAGAAAGGG CCTAACTTAT TTACATCTCG ACTACAATTT CAATTTAAAA 
CCAACTAAAA CCTTAACCAC AAAAGAACGT AAAAAGTCAA GACTTGGAAA CTCATTTCAT 
TTGATGCGTG AATTGCTAAA AATGATGAAA CTGATTGTAG ATACACATGT GCAGTTTAGG 
TTAGGAAATG TAGATGCCTT CCAGTTAGCA GATGGTATAC ATTATATTTT GAACCACATA 
GGACAATTGA CCGGTATTTA TCGTTATAAA TATAAAGTAA TGCATCAAAT CCGCGCATGT 
AAAGATCTGA AACATATCAT ATATTATAAG TTTAATAAAA ATTTGGGAAA AGGACCTGGT 
TGTGGATTTT GGCAACCTGC GTGGAGAGTG TGGCTAAATT TCTTAAGAGG CACTATACCA 
CTACTCGAAA GATATATTGG AAACCTTATC ACACGTCAAT TTGAAGGCCG TTCTAATGAA 
ATTGTCAAAA CTACAACTAA ACAAAGACTT GACGCCTACT ACGATCTTGA GTTACGAAAC 
TCGGTCATGG ATGACATTTT AGAAATGATG CCTGAGAGTA TTCGACAGAA AAAGGCAAGA 
ACTATACTGC AACATTTAAG TGAGGCTTGG AGATGTTGGA AGGCTAATAT TCCATGGGAT 
GTTCCTGGTA TGCCAGCCCC AATCAAAAAA ATAATTGAAC GGTATATAAA ATCCAAAGCT 
GATGCCTGGG TATCTGCAGC TCATTACAAT CGAGAACGTA TAAAGCGTGG AGCTCACGTC 
GAAAAGACTA TGGTTAAAAA AAATCTGGGG CGTTTAACGA GACTTTGGAT CAAAAATGAG 
CAGGAACGTC AAAGGCAAAT ACAGAAAAAC GGTCCCGAAA TTACTCCCGA AGAGGCTACG 
ACCATATTTT CTGTTATGGT TGAGTGGCTT GAATCAAGGA GCTTTTCACC AATACCATTT 
CCACCTTTAA CTTACAAAAA TGATACTAAG ATATTAGTTC TTGCCCTCGA AGACCTAAAA 
GATGTCTACG CATCAAAAGT ACGTTTAAAT GCATCTGAAA GGGAAGAACT TGCGTTGATA 
GAGGAGGCTT ATGATAATCC TCACGATACT TTGAACAGAA TCAAAAAGTA CTTGTTGACC 
CAGCGAGTTT TTAAGCCTGT TGATATAACC ATGATGGAAA ACTATCAAAA CATTTCCCCT 
GTTTATTCAG TTGATCCCTT GGAAAAGATT ACCGATGCAT ATCTTGATCA GTATTTATGG 
TACGAAGCTG ACCAACGAAA GCTTTTCCCA AACTGGATTA AACCAAGTGA TTCAGAGATA 
CCACCTCTTC TGGTATACAA GTGGACTCAG GGTATAAACA ACCTATCTGA AATTTGGGAT 
GTATCCAGAG GCCAATCTGC AGTTTTGCTT GAGACTACTT TGGGTGAAAT GGCCGAAAAA 
ATTGACTTTA CTTTACTTAA TAGATTACTT CGCCTGATTG TAGATCCTAA TATTGCTGAC 
TATATCACTG CAAAAAATAA TGTTGTTATC AACTTTAAAG ATATGAGTCA CGTCAACAAA 
TATGGCTTAA TACGCGGGTT GAAGTTCGCT TCTTTCATAT TCCAATATTA CGGACTAGTT 
ATAGATCTTT TACTATTGGG TCAGGAAAGG GCTACAGATT TGGCTGGTCC AGCTAACAAT 
CCAAATGAAT TTATGCAATT CAAGAGCAAA GAAGTAGAAA AGGCACATCC GATCAGACTT 
TACACCAGAT ATTTAGATCG TATATATATG CTTTTTCACT TTGAAGAAGA TGAGGGAGAG 
GAATTGACTG ATGAATACTT GGCAGAGAAT CCAGATCCAA ACTTTGAAAA CAGCATCGGA 
TACAATAATA GAAAATGCTG GCCCAAAGAT TCGCGCATGA GGCTAATACG TCAGGACGTT 
AACCTAGGGC GAGCAGTGTT TTGGGAAATT CAAAGTAGGG TGCCAACTTC GTTAACGTCA 
ATTAAATGGG AAAATGCTTT TGTTTCCGTA TACAGTAAAA ACAATCCGAA CTTACTCTTC 
TCTATGTGTG GTTTTGAAGT CAGAATATTA CCAAGACAAA GAATGGAAGA GGTGGTTTCT 
AACGATGAAG GTGTTTGGGA TTTAGTTGAC GAAAGAACCA AGCAAAGAAC TGCAAAAGCA 
TATTTAAAGG TATCTGAAGA AGAAATCAAA AAATTTGACA GCAGAATAAG AGGCATATTG 
ATGGCCTCCG GTTCAACCAC TTTTACTAAA GTGGCCGCTA AATGGAACAC TTCACTGATA 
TCCCTTTTCA CATACTTTAG AGAAGCCATT GTTGCCACGG AGCCGTTATT AGATATCCTG 
GTGAAAGGAG AAACAAGAAT TCAAAACCGT GTCAAACTTG GTTTAAATTC GAAAATGCCA 
ACAAGATTTC CACCTGCTGT TTTTTATACA CCAAAAGAAC TTGGTGGGTT GGGTATGATT 
AGTGCTTCTC ATATTTTAAT TCCTGCATCT GACTTAAGTT GGTCAAAACA GACCGATACT 
GGAATTACGC ATTTTCGTGC TGGTATGACG CACGAGGATG AAAAATTAAT CCCAACTATC 
TTCCGTTACA TCACTACTTG GGAAAATGAA TTTTTAGACT CTCAAAGGGT GTGGGCCGAG 
TATGCAACGA AAAGGCAGGA AGCTATTCAG CAGAATAGGA GGTTAGCTTT TGAAGAACTT 
GAAGGATCGT GGGATCGTGG TATACCTCGT ATTAGCACTT TATTTCAAAG AGATCGACAT 
ACTCTAGCGT ATGACAGAGG TCACAGAATC CGTAGGGAAT TTAAACAGTA CTCTCTAGAG 
AGAAACAGCC CCTTTTGGTG GACAAACTCC CACCATGACG GTAAATTATG GAATTTAAAT 
GCCTATAGAA CTGATGTGAT TCAAGCCCTA GGAGGTATTG AAACAATTTT GGAACACACT 
TTATTTAAGG GGACTGGTTT TAATTCATGG GAAGGTTTGT TTTGGGAAAA GGCGTCCGGG 
TTTGAGGATT CCATGCAATT TAAGAAGCTA ACTCATGCTC AAAGAACTGG TTTAAGCCAA 
ATTCCAAATC GTAGATTTAC CTTATGGTGG TCACCAACTA TTAACAGAGC AAATGTCTAT 
GTCGGTTTCT TGGTGCAATT GGATCTGACT GGTATCTTCC TCCATGGTAA AATTCCTACG 
TTAAAAATAT CTTTAATTCA AATATTCCGT GCTCATTTGT GGCAAAAAAT ACATGAAAGT 
ATAGTTTTTG ATATTTGTCA AATTCTCGAT GGTGAGCTAG ATGTTTTGCA AATTGAATCT 
GTGACTAAAG AAACAGTGCA TCCCCGCAAA TCGTACAAGA TGAATTCATC TGCCGCTGAC 
ATAACTATGG AAAGTGTTCA TGAATGGGAA GTTTCAAAAC CCTCCCTATT ACATGAAACA 
AATGACTCAT TTAAGGGATT GATTACCAAC AAAATGTGGT TTGATGTACA ATTGAGATAC 
GGTGATTATG ATTCTCATGA TATATCTCGT TATGTGAGGG CAAAATTCCT CGATTATACA 
ACAGATAATG TCAGCATGTA TCCCTCCCCA ACAGGTGTTA TGATCGGTAT CGATTTGGCT 
TATAACATGT ATGATGCCTA TGGTAATTGG TTCAACGGAT TAAAACCTTT GATACAAAAT 
AGTATGAGAA CAATTATGAA AGCTAACCCA GCATTATATG TCCTTCGTGA ACGTATTAGA 
AAAGGTCTTC AAATATATCA ATCTAGTGTA CAGGAACCAT TTTTAAACTC CTCAAACTAT 
GCCGAGTTAT TCAATAACGA CATTAAACTC TTCGTTGACG ACACTAATGT GTATAGAGTT 
ACTGTCCACA AGACTTTTGA AGGGAACGTT GCTACAAAAG CAATTAATGG TTGCATTTTC 
ACTTTAAATC CAAAGACTGG GCATCTGTTT CTGAAAATTA TCCATACCTC TGTATGGGCC 
GGTCAAAAAC GTTTAAGCCA ATTGGCCAAA TGGAAAACAG CTGAGGAAGT TAGTGCTCTT 
GTGAGATCTT TGCCCAAAGA AGAGCAGCCA AAACAAATTA TCGTCACAAG AAAGGCAATG 
CTTGATCCTT TAGAGGTTCA CATGCTTGAC TTTCCTAACA TAGCAATCAG GCCAACAGAG 
CTGCGACTAC CATTTTCAGC TGCAATGTCA ATAGATAAAC TTTCTGATGT TGTGATGAAA 
GCTACTGAAC CTCAGATGGT TCTGTTTAAC ATTTATGATG ATTGGTTAGA CCGTATTTCA 
TCGTACACTG CATTTTCTAG ACTTACACTA CTTTTAAGAG CTTTAAAGAC GAACGAGGAA 
AGTGCCAAAA TGATTCTACT CAGTGACCCC ACGATCACTA TCAAATCGTA CCATTTATGG 
CCTTCATTCA CTGATGAACA ATGGATCACT ATCGAATCTC AAATGAGAGA TTTGATTTTG 
ACTGAGTACG GCAGAAAATA CAATGTCAAT ATATCTGCCT TAACTCAAAC GGAAATTAAA 
GATATTATAT TGGGTCAAAA TATCAAGGCA CCATCTGTGA AGAGACAAAA AATGGCTGAA 
TTAGAAGCTG CCAGGTCAGA AAAGCAGAAC GACGAAGAAG CTGCAGGTGC ATCAACTGTT 
ATGAAAACTA AAACTATCAA TGCGCAAGGT GAGGAGATAG TTGTAGTAGC TTCCGCTGAT 
TACGAAAGTC AAACGTTTAG TTCAAAGAAT GAATGGAGAA AATCTGCAAT TGCTAATACA 
CTATTATATT TGAGGTTAAA AAACATTTAT GTTTCGGCTG ATGATTTCGT GGAGGAACAG 
AATGTTTACG TCCTTCCAAA AAATTTATTA AAGAAGTTTA TTGAGATATC CGATGTGAAA 
ATCCAGGTGG CGGCCTTTAT TTATGGTATG TCAGCAAAAG ATCATCCGAA GGTGAAAGAA 
ATCAAGACTG TTGTGCTGGT ACCTCAATTA GGCCACGTAG GATCCGTTCA GATAAGCAAC 
ATCCCTGACA TCGGAGACCT TCCGGATACT GAAGGCCTTG AACTACTCGG TTGGATCCAT 
ACCCAGACAG AAGAACTTAA ATTCATGGCT GCGTCTGAAG TAGCAACTCA TTCTAAACTT 
TTTGCGGATA AGAAAAGAGA CTGCATTGAT ATTTCAATTT TTTCCACCCC GGGATCAGTA 
TCATTAAGCG CTTATAACCT GACCGACGAA GGTTACCAAT GGGGTGAAGA AAATAAGGAT 
ATAATGAACG TGCTATCGGA AGGGTTTGAG CCTACTTTCA GCACGCATGC CCAATTACTT 
CTCTCTGACC GTATCACAGG TAATTTTATA ATTCCATCTG GAAACGTTTG GAACTACACA 
TTTATGGGAA CAGCATTTAA CCAAGAAGGT GACTACAATT TTAAATACGG CATTCCTTTG 
GAATTCTATA ATGAGATGCA TCGTCCAGTA CACTTTTTAC AGTTTAGCGA ATTGGCGGGG 
GACGAAGAGT TAGAGGCCGA ACAAATCGAT GTATTTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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PRP8 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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