myo52 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	myo52
Entrez Gene ID	2538846
Full Name	myosin type V
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001023482.2	NP_588492.1	myosin type V

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	myo5b	XP_002936554.2
Pan troglodytes (chimpanzee)	MYO5B	XP_003953333.1
Oryza sativa (rice)	Os02g0777700	NP_001048291.2
	MYO5B	XP_001090434.1
Canis lupus familiaris (dog)	MYO5B	XP_005623182.1
Bos taurus (cattle)	MYO5B	XP_002697867.2
Mus musculus (house mouse)	Myo5b	NP_963894.1
Danio rerio (zebrafish)	myo5b	NP_001155104.1
Schizosaccharomyces pombe (fission yeast)	myo52	NP_588492.1
Arabidopsis thaliana (thale cress)	XI-I	NP_195046.3
Homo sapiens (human)	MYO5B	NP_001073936.1
Rattus norvegicus (Norway rat)	Myo5b	NP_058779.1
Danio rerio (zebrafish)	LOC100536519	XP_003200915.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rng8 and Rng9 accumulate on the fusion focus, dependent on Myo51 and tropomyosin Cdc8. A tropomyosin mutant allele, which compromises Rng8/9 localization but not actin binding, similarly leads to multiple stable dots instead of a single focus. By contrast, myo51 deletion does not strongly affect fusion focus coalescence
Title: A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.

Myo52p lacking cargo, or single molecules of wild-type Myo52p with small cargoes, can undergo processive movement along actin-Cdc8p cables in vivo.
Title: Fission yeast tropomyosin specifies directed transport of myosin-V along actin cables.

Myo52 motor activity may pull on cables to provide the tension necessary for their extension and efficient assembly, as artificially tethering actin cables to the nuclear envelope via a Myo52 motor domain
Title: Myosin Vs organize actin cables in fission yeast.

Myo52 facilitates microtubule catastrophe by enhancing Tip1 removal from the plus end of growing microtubules at the cell tips.
Title: Myosin V spatially regulates microtubule dynamics and promotes the ubiquitin-dependent degradation of the fission yeast CLIP-170 homologue, Tip1.

In vivo movement of the type V myosin Myo52 requires dimerization but is independent of the neck domain.
Title: In vivo movement of the type V myosin Myo52 requires dimerisation but is independent of the neck domain.

The following myo52 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myo52 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99603	NM_001023482.2 Latest version!	Schizosaccharomyces pombe myosin type V (myo52), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myo52 ( NM_001023482.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_588492.1 Schizosaccharomyces pombe myosin type V (myo52), mRNA.

Price & Availability↑

CloneID	OSc99603
Clone ID Related Accession (Same CDS sequence)	NM_001023482.2
Accession Version	NM_001023482.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	myosin type V
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023482.1.

ATGACATCGG GGATTTATTA CAAGGGACTT CAATGCTGGA TTCCAGATGA GCAAAGCCAA 
TGGATCCCTG GAAGCATAAA GGATTGTCGA GTTGAAGGTG AGAAAGCTTT CTTAACGGTA 
CAAGATGAAA ACGAAAACGA AACTGTTATT ACAGTGAAGC CAGACGATTT AAATTATGAA 
GGAAGAAACG GCCTCCCGTT TCTTCGATCC ATTAACTCGG ATGCTGATGA CCTTACTGAT 
CTTTCATACC TAAATGAGCC GTCAGTGTTG GATGCTTTGT CAACAAGGTA CAACCAACTT 
CAAATTTATA CGTATTCTGG CATTGTCCTT ATCGCAGTAA ATCCTTTTCA GAGACTGCCA 
AATTTGTACA CTCACGAAAT AGTACGTGCT TATTCGGAGA AATCTCGGGA TGAGCTAGAT 
CCCCATCTCT ATGCTATTGC TGAAGATAGC TACAAGTGCA TGAATCAAGA GCATAAAAAT 
CAAACTATTA TTATATCTGG TGAGTCAGGA GCAGGAAAAA CTGTTTCCGC AAGATACATA 
ATGCGGTATT TTGCTTCTGT GCAAGCACTT ATTCAATCCA CCGATTCCAA TTTTCATGAA 
GCTCCACAAT TAACTGCTGT CGAGAATGAG ATACTTGCTA CAAATCCGAT TATGGAGGCT 
TTTGGTAATT CTAAAACTTC TAGAAATGAC AATTCTTCTC GCTTTGGTAA ATATATTCAA 
ATTTTGTTCG ACGGAAATGC TACAATCATT GGTGCTAAAA TTCAAACTTA TCTTTTAGAA 
AGATCAAGAT TAGTATTTCA GCCTAATCAA GAAAGAAATT ATCATATTTT CTATCAGATT 
TTAGCTGGTT CATCCTCGGA ACAACTTGAA AAGTGGAAAC TTGTTGAGAA TTCTCAAGAA 
TTTAATTATT TAAAACAGGG AAATTGTTCT ACAATTGAGG GCGTCAATGA TAAAGAGGAA 
TTTAAAGCTA CAGTTGATGC ATTAAAAACA GTTGGCATTG ATAATGATAC ATGTGAATGC 
ATATTTAGCT TATTAGCTGC ATTGCTGCAT ATCGGTAACA TAGAGGTTAA GCATTCAAGG 
AATGATGCAT ACATTGATTC AAAAAACGAA AATCTGATAA ACGCTACTTC ATTGTTGGGC 
GTGGATCCTT CTTCATTGGT GAAATGGTTA ACGAAGCGTA AAATAAAGAT GGCCTCTGAA 
GGTATTTTGA AACCATTGAA CGAATTTCAA GCTGTAGTAG CTAGAGACTC AGTAGCGAAA 
TTCCTTTACG CTTCACTCTT CGACTGGCTG GTAGCTACTA TTAATAAAGC CTTGATGTAC 
TCAGCTGATA AATCGAACCA GACGGCTAAA AGTTTTATTG GTGTTCTAGA TATTTATGGG 
TTCGAGCACT TTAAAAAAAA TTCATTTGAA CAGTTTTGCA TCAATTATGC GAACGAAAAA 
CTTCAACAGG AATTTTATCG CCATGTTTTC AAACTGGAAC AGGAGGAGTA TGCTGCTGAG 
GGACTTAACT GGTCCTATAT TGATTATCAA GACAATCAGC AATGTATTAG CATGATTGAG 
AGTCGTTTAG GGATTCTTTC CCTTTTAGAC GAAGAATGCC GAATGCCCAC GAACAGCGAT 
GAAAATTGGG TTAGTAAGTT GAACGATGCA TTCTCCAAGC CAGAATTCAA AAATAGTTAT 
CAGAAATCAA GGTTTGGTAA CAAGGAGTTT ACAATTAAGC ATTATGCTCT TGACGTTGTT 
TACTGTGCCG AAGGATTTAT TGATAAAAAC CGTGATACAA TATCCGATGA GTTGTTGGAG 
CTTTTTACAA ATTCAGACGT TCCCTTTGTC AAGGATCTGG TTTTATTCCG TCTTGAACAA 
ACTGCACCCC CAGCCGATAC CAAAAAAATA AAAACCAAGC CAAAAAGTAA TACTCTTGGT 
TCAATGTTTA AAAGCTCTTT GGTTAGTCTA ATGAGTACCA TCAACGAAAC TAATGCTCAT 
TATATTCGTT GTATCAAGCC AAATGAAGAG AAAGAAGCCT GGAAATTTGA TAACCAAATG 
GTGGTTTCTC AGTTACGAGC ATGCGGTGTT TTAGAAACTA TAAAAATATC TTGTGCTGGT 
TTTCCCTCCC GTTGGACTTT TGATGAGTTT GTTTCTCGTT ATTACATGTT GGTTCCCTCT 
GCCGTAAGAA CTACGGAGTC TTTGACCTTT AGCAAGGCAA TTTTAGAGAA ACATGCTGAT 
CCGACTAAAT ATCAAATTGG CAAAACTAAA ATATTTTTTC GTTCAGGTGT GACGCCCCTT 
TTAGAGAGTG CGAGAGACAA AGCTTTGAAA CATGCAGCAC ATCTTTTGTA TGAAGCGTTT 
GCTGTTAATT ACTATAGGAC TCGTTTTCTT CTGTCTCGGA AGAGAGTCAG GAGCTTTCAA 
GCCGTTGCTC ATGGTTTCTT ATCTCGTCGC CATACGGAGT ACGAATTACT TTCTTCCAAT 
ATTATTAAAC TTCAGTCACT TTGGAGAACT GCATTAAAGA GAAAAGAATT CATACAAACG 
AAAAATTCTA TACTTAAAGT GCAAAGTATA ATTAGAGGCT TTTTATTAAG ACAGACTTTA 
GAGGAAAAGA CAAAGCATGA TGCAACATTA ATTATTCAAA GTCTTTGGTT AACATTTAAA 
GCCCATAAGC ACTACAAAGA ACTACAATAT TATGCAGTTC GTATCCAGTC TCTTTGGAGG 
ATGAAACTTG CAAAGCGACA ATTGACAGAA TTAAAAATTG AATCCACTAA AGCGAGTCAC 
CTTAAGCAAG TATCTTACAG GCTTGAAAGC AGATTGTTTG AGATTTCAAA ACAGTTGGAC 
AACTCGGAGC AGGAAAATAA CAAGTTCAGG GAAAGAATCG CTGAATTGGA GTCTCATCTG 
TCCAACTATG CTGAAGCAAA GTTAGCACAA GAAAGAGAAC TTGAGCAAAC TAGGGTACTT 
ATATCGGATC AATCTCAAGA TGGGGAATTA AAGGAGTTAC TTGAAGAGAA AGAAAACGCT 
TTGATTATGA TGGAAGAGGA GATGAGGCAA GTCAACGATG CGAATACCGA ACTTTTAAGA 
GTAAATGCAA CTTTGAAGTC GCAGCTTAAA AATTACGATA TGATTATTGT TGAGCAAACC 
TCTCAGTTGA AAGAGAAAAA CAGGATAATT GCTAGTCTGA CAAAAGCCAC TAAAATACTG 
AATTCGGCCT CTAGTATCGA ACAGTCGCGC AATTCGGAAG AGAAATCACG TCGTGATAGT 
TCATTGATGG AAATGCGTAC TCAAAAAGAG ATGCTTGTGC TATTAATGAA CGATGGCTTG 
AAGCACGATC TTGACAAACT CACAGAATAT GCAGGTAGAA CATTTACAAC TCTTAAAACT 
CTATTACTAA AGGACAACGA TGTTGAGGCT CAGAAACTTG ATCATTTGTT TTTAGCAAAA 
CTACTTTTTA TAATAATATC ACAAATGTGG AAATCAAATC TGTGTCAGGA AAGTGTTGCC 
TTGGTTGAGC GTTACTGTGT CCACACTTTA GAATACGTAT TTCAAAAGAC ATCATCTGCT 
AATGAGAGAC CTGACATCGG ATTTTGGGTT GCAAATACAC ATGCGCTCTT GGCTTTTGTT 
TATACTAAGC AGCAGGCGTT TAAACATTCC TCGGCTTTCA CTCTTTTATC CACCGAATCT 
CATGAATCAG TGCAAACCAT TTTTGAGATG ATTGAATCAC ATCTGTCAAA AATCTTTTTC 
GAATGGGTTA GGCAAGTCAA TAATTTTTTA AAGCCATTGA TTGTTCAGGC AATGATTATT 
ACTGGTACAA ATACGGATGC TGGAGATGAG AATCGAAAAC TACGGATTAA ATTTTTTGAG 
AAACCAAAAT ACAAAATCAC CGATGTAATA CATGTACTAA ATAAGGTGCA TGATTCTTGT 
CAGGCCTATA AAGTTAATTA TGAAATATAT AATGCATTGA TTAGAAGCAT ATATCGATTC 
ATTAATGTGG AGGCATTCAA TTCACTTTTT ATTGATGAAC GTGGATCTTG GAAACGTGGT 
ACCAATATTT CATACAATTA TCATGTTCTT AAGGATTGGT GTCTAGAAAG TGGTGTTCCG 
GAAGCTTATT TGCAGCTCGA AGAGCTTTTA CAAACCTCCA AGATACTACA ATTTGTGAAA 
GATGATCCAA ATTATGTGGC CAGGGTCCGC GACTTTTATG CTCTTAATTT TCTGCAAATA 
AAAACACTCT TACATCGTTA TGACTATGCT GACTACGAGG CTCATGTACC GAAAAAAACT 
ATGTCTGAAT TGTCCAAAAA TATTGTTGCT GAAGGCATCA ATCAAAGGGA ACAACTTACT 
TACGAAGTGT TGGATTATAG ACTACAAGAT AGTTTTGAGG AATCTCCATC TCTTGAAAAA 
ATAAAGATAC CAGATGATTG TAATGTTACC TATTTGCGTC GTATCATCGA CCTTGCTAGT 
GCAGAAGAAA GTGTTGAGCA AGCTTTGATC ACTGTAGGCA ACGTAGCCGA CAATGATGTA 
CAGAACTCGA GCGACGAAGA AAATCAAGTA CCAAATGGTA TTAAAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_588492.1
CDS	202..4752
Translation	MTSGIYYKGLQCWIPDEQSQWIPGSIKDCRVEGEKAFLTVQDENENETVITVKPDDLNYEGRNGLPFLRSINSDADDLTDLSYLNEPSVLDALSTRYNQLQIYTYSGIVLIAVNPFQRLPNLYTHEIVRAYSEKSRDELDPHLYAIAEDSYKCMNQEHKNQTIIISGESGAGKTVSARYIMRYFASVQALIQSTDSNFHEAPQLTAVENEILATNPIMEAFGNSKTSRNDNSSRFGKYIQILFDGNATIIGAKIQTYLLERSRLVFQPNQERNYHIFYQILAGSSSEQLEKWKLVENSQEFNYLKQGNCSTIEGVNDKEEFKATVDALKTVGIDNDTCECIFSLLAALLHIGNIEVKHSRNDAYIDSKNENLINATSLLGVDPSSLVKWLTKRKIKMASEGILKPLNEFQAVVARDSVAKFLYASLFDWLVATINKALMYSADKSNQTAKSFIGVLDIYGFEHFKKNSFEQFCINYANEKLQQEFYRHVFKLEQEEYAAEGLNWSYIDYQDNQQCISMIESRLGILSLLDEECRMPTNSDENWVSKLNDAFSKPEFKNSYQKSRFGNKEFTIKHYALDVVYCAEGFIDKNRDTISDELLELFTNSDVPFVKDLVLFRLEQTAPPADTKKIKTKPKSNTLGSMFKSSLVSLMSTINETNAHYIRCIKPNEEKEAWKFDNQMVVSQLRACGVLETIKISCAGFPSRWTFDEFVSRYYMLVPSAVRTTESLTFSKAILEKHADPTKYQIGKTKIFFRSGVTPLLESARDKALKHAAHLLYEAFAVNYYRTRFLLSRKRVRSFQAVAHGFLSRRHTEYELLSSNIIKLQSLWRTALKRKEFIQTKNSILKVQSIIRGFLLRQTLEEKTKHDATLIIQSLWLTFKAHKHYKELQYYAVRIQSLWRMKLAKRQLTELKIESTKASHLKQVSYRLESRLFEISKQLDNSEQENNKFRERIAELESHLSNYAEAKLAQERELEQTRVLISDQSQDGELKELLEEKENALIMMEEEMRQVNDANTELLRVNATLKSQLKNYDMIIVEQTSQLKEKNRIIASLTKATKILNSASSIEQSRNSEEKSRRDSSLMEMRTQKEMLVLLMNDGLKHDLDKLTEYAGRTFTTLKTLLLKDNDVEAQKLDHLFLAKLLFIIISQMWKSNLCQESVALVERYCVHTLEYVFQKTSSANERPDIGFWVANTHALLAFVYTKQQAFKHSSAFTLLSTESHESVQTIFEMIESHLSKIFFEWVRQVNNFLKPLIVQAMIITGTNTDAGDENRKLRIKFFEKPKYKITDVIHVLNKVHDSCQAYKVNYEIYNALIRSIYRFINVEAFNSLFIDERGSWKRGTNISYNYHVLKDWCLESGVPEAYLQLEELLQTSKILQFVKDDPNYVARVRDFYALNFLQIKTLLHRYDYADYEAHVPKKTMSELSKNIVAEGINQREQLTYEVLDYRLQDSFEESPSLEKIKIPDDCNVTYLRRIIDLASAEESVEQALITVGNVADNDVQNSSDEENQVPNGIKV

Target ORF information:

RefSeq Version	NM_001023482.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe myosin type V (myo52), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001023482.2

ORF Insert Sequence:

ATGACATCGG GGATTTATTA CAAGGGACTT CAATGCTGGA TTCCAGATGA GCAAAGCCAA 
TGGATCCCTG GAAGCATAAA GGATTGTCGA GTTGAAGGTG AGAAAGCTTT CTTAACGGTA 
CAAGATGAAA ACGAAAACGA AACTGTTATT ACAGTGAAGC CAGACGATTT AAATTATGAA 
GGAAGAAACG GCCTCCCGTT TCTTCGATCC ATTAACTCGG ATGCTGATGA CCTTACTGAT 
CTTTCATACC TAAATGAGCC GTCAGTGTTG GATGCTTTGT CAACAAGGTA CAACCAACTT 
CAAATTTATA CGTATTCTGG CATTGTCCTT ATCGCAGTAA ATCCTTTTCA GAGACTGCCA 
AATTTGTACA CTCACGAAAT AGTACGTGCT TATTCGGAGA AATCTCGGGA TGAGCTAGAT 
CCCCATCTCT ATGCTATTGC TGAAGATAGC TACAAGTGCA TGAATCAAGA GCATAAAAAT 
CAAACTATTA TTATATCTGG TGAGTCAGGA GCAGGAAAAA CTGTTTCCGC AAGATACATA 
ATGCGGTATT TTGCTTCTGT GCAAGCACTT ATTCAATCCA CCGATTCCAA TTTTCATGAA 
GCTCCACAAT TAACTGCTGT CGAGAATGAG ATACTTGCTA CAAATCCGAT TATGGAGGCT 
TTTGGTAATT CTAAAACTTC TAGAAATGAC AATTCTTCTC GCTTTGGTAA ATATATTCAA 
ATTTTGTTCG ACGGAAATGC TACAATCATT GGTGCTAAAA TTCAAACTTA TCTTTTAGAA 
AGATCAAGAT TAGTATTTCA GCCTAATCAA GAAAGAAATT ATCATATTTT CTATCAGATT 
TTAGCTGGTT CATCCTCGGA ACAACTTGAA AAGTGGAAAC TTGTTGAGAA TTCTCAAGAA 
TTTAATTATT TAAAACAGGG AAATTGTTCT ACAATTGAGG GCGTCAATGA TAAAGAGGAA 
TTTAAAGCTA CAGTTGATGC ATTAAAAACA GTTGGCATTG ATAATGATAC ATGTGAATGC 
ATATTTAGCT TATTAGCTGC ATTGCTGCAT ATCGGTAACA TAGAGGTTAA GCATTCAAGG 
AATGATGCAT ACATTGATTC AAAAAACGAA AATCTGATAA ACGCTACTTC ATTGTTGGGC 
GTGGATCCTT CTTCATTGGT GAAATGGTTA ACGAAGCGTA AAATAAAGAT GGCCTCTGAA 
GGTATTTTGA AACCATTGAA CGAATTTCAA GCTGTAGTAG CTAGAGACTC AGTAGCGAAA 
TTCCTTTACG CTTCACTCTT CGACTGGCTG GTAGCTACTA TTAATAAAGC CTTGATGTAC 
TCAGCTGATA AATCGAACCA GACGGCTAAA AGTTTTATTG GTGTTCTAGA TATTTATGGG 
TTCGAGCACT TTAAAAAAAA TTCATTTGAA CAGTTTTGCA TCAATTATGC GAACGAAAAA 
CTTCAACAGG AATTTTATCG CCATGTTTTC AAACTGGAAC AGGAGGAGTA TGCTGCTGAG 
GGACTTAACT GGTCCTATAT TGATTATCAA GACAATCAGC AATGTATTAG CATGATTGAG 
AGTCGTTTAG GGATTCTTTC CCTTTTAGAC GAAGAATGCC GAATGCCCAC GAACAGCGAT 
GAAAATTGGG TTAGTAAGTT GAACGATGCA TTCTCCAAGC CAGAATTCAA AAATAGTTAT 
CAGAAATCAA GGTTTGGTAA CAAGGAGTTT ACAATTAAGC ATTATGCTCT TGACGTTGTT 
TACTGTGCCG AAGGATTTAT TGATAAAAAC CGTGATACAA TATCCGATGA GTTGTTGGAG 
CTTTTTACAA ATTCAGACGT TCCCTTTGTC AAGGATCTGG TTTTATTCCG TCTTGAACAA 
ACTGCACCCC CAGCCGATAC CAAAAAAATA AAAACCAAGC CAAAAAGTAA TACTCTTGGT 
TCAATGTTTA AAAGCTCTTT GGTTAGTCTA ATGAGTACCA TCAACGAAAC TAATGCTCAT 
TATATTCGTT GTATCAAGCC AAATGAAGAG AAAGAAGCCT GGAAATTTGA TAACCAAATG 
GTGGTTTCTC AGTTACGAGC ATGCGGTGTT TTAGAAACTA TAAAAATATC TTGTGCTGGT 
TTTCCCTCCC GTTGGACTTT TGATGAGTTT GTTTCTCGTT ATTACATGTT GGTTCCCTCT 
GCCGTAAGAA CTACGGAGTC TTTGACCTTT AGCAAGGCAA TTTTAGAGAA ACATGCTGAT 
CCGACTAAAT ATCAAATTGG CAAAACTAAA ATATTTTTTC GTTCAGGTGT GACGCCCCTT 
TTAGAGAGTG CGAGAGACAA AGCTTTGAAA CATGCAGCAC ATCTTTTGTA TGAAGCGTTT 
GCTGTTAATT ACTATAGGAC TCGTTTTCTT CTGTCTCGGA AGAGAGTCAG GAGCTTTCAA 
GCCGTTGCTC ATGGTTTCTT ATCTCGTCGC CATACGGAGT ACGAATTACT TTCTTCCAAT 
ATTATTAAAC TTCAGTCACT TTGGAGAACT GCATTAAAGA GAAAAGAATT CATACAAACG 
AAAAATTCTA TACTTAAAGT GCAAAGTATA ATTAGAGGCT TTTTATTAAG ACAGACTTTA 
GAGGAAAAGA CAAAGCATGA TGCAACATTA ATTATTCAAA GTCTTTGGTT AACATTTAAA 
GCCCATAAGC ACTACAAAGA ACTACAATAT TATGCAGTTC GTATCCAGTC TCTTTGGAGG 
ATGAAACTTG CAAAGCGACA ATTGACAGAA TTAAAAATTG AATCCACTAA AGCGAGTCAC 
CTTAAGCAAG TATCTTACAG GCTTGAAAGC AGATTGTTTG AGATTTCAAA ACAGTTGGAC 
AACTCGGAGC AGGAAAATAA CAAGTTCAGG GAAAGAATCG CTGAATTGGA GTCTCATCTG 
TCCAACTATG CTGAAGCAAA GTTAGCACAA GAAAGAGAAC TTGAGCAAAC TAGGGTACTT 
ATATCGGATC AATCTCAAGA TGGGGAATTA AAGGAGTTAC TTGAAGAGAA AGAAAACGCT 
TTGATTATGA TGGAAGAGGA GATGAGGCAA GTCAACGATG CGAATACCGA ACTTTTAAGA 
GTAAATGCAA CTTTGAAGTC GCAGCTTAAA AATTACGATA TGATTATTGT TGAGCAAACC 
TCTCAGTTGA AAGAGAAAAA CAGGATAATT GCTAGTCTGA CAAAAGCCAC TAAAATACTG 
AATTCGGCCT CTAGTATCGA ACAGTCGCGC AATTCGGAAG AGAAATCACG TCGTGATAGT 
TCATTGATGG AAATGCGTAC TCAAAAAGAG ATGCTTGTGC TATTAATGAA CGATGGCTTG 
AAGCACGATC TTGACAAACT CACAGAATAT GCAGGTAGAA CATTTACAAC TCTTAAAACT 
CTATTACTAA AGGACAACGA TGTTGAGGCT CAGAAACTTG ATCATTTGTT TTTAGCAAAA 
CTACTTTTTA TAATAATATC ACAAATGTGG AAATCAAATC TGTGTCAGGA AAGTGTTGCC 
TTGGTTGAGC GTTACTGTGT CCACACTTTA GAATACGTAT TTCAAAAGAC ATCATCTGCT 
AATGAGAGAC CTGACATCGG ATTTTGGGTT GCAAATACAC ATGCGCTCTT GGCTTTTGTT 
TATACTAAGC AGCAGGCGTT TAAACATTCC TCGGCTTTCA CTCTTTTATC CACCGAATCT 
CATGAATCAG TGCAAACCAT TTTTGAGATG ATTGAATCAC ATCTGTCAAA AATCTTTTTC 
GAATGGGTTA GGCAAGTCAA TAATTTTTTA AAGCCATTGA TTGTTCAGGC AATGATTATT 
ACTGGTACAA ATACGGATGC TGGAGATGAG AATCGAAAAC TACGGATTAA ATTTTTTGAG 
AAACCAAAAT ACAAAATCAC CGATGTAATA CATGTACTAA ATAAGGTGCA TGATTCTTGT 
CAGGCCTATA AAGTTAATTA TGAAATATAT AATGCATTGA TTAGAAGCAT ATATCGATTC 
ATTAATGTGG AGGCATTCAA TTCACTTTTT ATTGATGAAC GTGGATCTTG GAAACGTGGT 
ACCAATATTT CATACAATTA TCATGTTCTT AAGGATTGGT GTCTAGAAAG TGGTGTTCCG 
GAAGCTTATT TGCAGCTCGA AGAGCTTTTA CAAACCTCCA AGATACTACA ATTTGTGAAA 
GATGATCCAA ATTATGTGGC CAGGGTCCGC GACTTTTATG CTCTTAATTT TCTGCAAATA 
AAAACACTCT TACATCGTTA TGACTATGCT GACTACGAGG CTCATGTACC GAAAAAAACT 
ATGTCTGAAT TGTCCAAAAA TATTGTTGCT GAAGGCATCA ATCAAAGGGA ACAACTTACT 
TACGAAGTGT TGGATTATAG ACTACAAGAT AGTTTTGAGG AATCTCCATC TCTTGAAAAA 
ATAAAGATAC CAGATGATTG TAATGTTACC TATTTGCGTC GTATCATCGA CCTTGCTAGT 
GCAGAAGAAA GTGTTGAGCA AGCTTTGATC ACTGTAGGCA ACGTAGCCGA CAATGATGTA 
CAGAACTCGA GCGACGAAGA AAATCAAGTA CCAAATGGTA TTAAAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
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myo52 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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