Myo5b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myo5b
Entrez Gene ID	17919
Full Name	myosin VB
Synonyms	AI661750,mKIAA1119
General protein information	Preferred Names myosin VB Names unconventional myosin-Vb myosin-Vb
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 E2\|18 50.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006525716.3	XP_006525779.1	unconventional myosin-Vb isoform X3
XM_006525714.4	XP_006525777.1	unconventional myosin-Vb isoform X1
XM_006525714.3	XP_006525777.1	unconventional myosin-Vb isoform X1
XM_006525715.3	XP_006525778.1	unconventional myosin-Vb isoform X2
NM_201600.2	NP_963894.1	unconventional myosin-Vb
XM_006525715.4	XP_006525778.1	unconventional myosin-Vb isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	myo5b	XP_002936554.2
Pan troglodytes (chimpanzee)	MYO5B	XP_003953333.1
Oryza sativa (rice)	Os02g0777700	NP_001048291.2
	MYO5B	XP_001090434.1
Canis lupus familiaris (dog)	MYO5B	XP_005623182.1
Bos taurus (cattle)	MYO5B	XP_002697867.2
Mus musculus (house mouse)	Myo5b	NP_963894.1
Danio rerio (zebrafish)	myo5b	NP_001155104.1
Schizosaccharomyces pombe (fission yeast)	myo52	NP_588492.1
Arabidopsis thaliana (thale cress)	XI-I	NP_195046.3
Homo sapiens (human)	MYO5B	NP_001073936.1
Rattus norvegicus (Norway rat)	Myo5b	NP_058779.1
Danio rerio (zebrafish)	LOC100536519	XP_003200915.2

Gene Ontology
Bibliography

Related articles in PubMed

Kinetic signatures of myosin-5B, the motor involved in microvillus inclusion disease.
Heissler SM, Chinthalapudi K, Sellers JR
The Journal of biological chemistry292(44)18372-18385(2017 11)

Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice.
Liu TY, Chen YC, Jong YJ, Tsai HJ, Lee CC, Chang YS, Chang JG, Chang YF
Open biology7(1)(2017 Jan)

Loss of MYO5B in mice recapitulates Microvillus Inclusion Disease and reveals an apical trafficking pathway distinct to neonatal duodenum.
Weis VG, Knowles BC, Choi E, Goldstein AE, Williams JA, Manning EH, Roland JT, Lapierre LA, Goldenring JR
Cellular and molecular gastroenterology and hepatology2(2)131-157(2016 02)

An inducible mouse model for microvillus inclusion disease reveals a role for myosin Vb in apical and basolateral trafficking.
Schneeberger K, Vogel GF, Teunissen H, van Ommen DD, Begthel H, El Bouazzaoui L, van Vugt AH, Beekman JM, Klumperman J, M?ller T, Janecke A, Gerner P, Huber LA, Hess MW, Clevers H, van Es JH, Nieuwenhuis EE, Middendorp S
Proceedings of the National Academy of Sciences of the United States of America112(40)12408-13(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that single-headed myosin-5B is intermediate-duty ratio motor with kinetic ATPase cycle, rate-limited by phosphate release; in transition to high-duty ratio motor, second head generates strain/gating in myosin-5B dimer that alter kinetic signature by slowing actin-activated ADP release to become rate-limiting; myosin-5B mutations cause microvillus inclusion disease and impact myosin motor function.
Title: Kinetic signatures of myosin-5B, the motor involved in microvillus inclusion disease.

Data show that loss of myosin Vb (MYO5B) disturbs both apical and basolateral trafficking of proteins and causes microvillus inclusion disease (MVID).
Title: An inducible mouse model for microvillus inclusion disease reveals a role for myosin Vb in apical and basolateral trafficking.

Myo5b knockout mice closely resemble the phenotype of MVID patients and constitute a useful model to further investigate the underlying molecular mechanism of mirovillus inclusion disease.
Title: Myo5b knockout mice as a model of microvillus inclusion disease.

MYO5B plays a role in the surface trafficking of Kv1.5 and connexin-43 but not KCNH2. MYO5B regulated postendocytic recycling.
Title: Role for myosin-V motor proteins in the selective delivery of Kv channel isoforms to the membrane surface of cardiac myocytes.

Acute recruitment of myosin-V to kinesin-propelled cargo reduces their motility near the cell periphery and enhances their localization at the actin-rich cell cortex.
Title: Myosin-V opposes microtubule-based cargo transport and drives directional motility on cortical actin.

The following Myo5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myo5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu54430	XM_006525714.4 Latest version!	Mus musculus myosin VB (Myo5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu54431	XM_006525715.4 Latest version!	Mus musculus myosin VB (Myo5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu22045	NM_201600.2 Latest version!	Mus musculus myosin VB (Myo5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu54432	XM_006525716.3 Latest version!	Mus musculus myosin VB (Myo5b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu54430	XM_006525714.3	Mus musculus myosin VB (Myo5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu54431	XM_006525715.3	Mus musculus myosin VB (Myo5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myo5b ( XM_006525714.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525777.1 Mus musculus myosin VB (Myo5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54430
Clone ID Related Accession (Same CDS sequence)	XM_006525714.4 , XM_006525714.3
Accession Version	XM_006525714.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Vb isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525714.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTACA GCGAGCTCTA CACCCGGTAC ACAAGGGTCT GGATCCCTGA CCCAGATGAG 
GTGTGGCGCT CGGCTGAATT AACCAAGGAC TACAAAGAAG GGGATAAGAG CCTGCAGCTC 
AGACTAGAAG ATGACACAAT TCTAGAATAC CCAGTTGATG TCCAAAATAA CCAGGTGCCA 
TTCTTGCGGA ATCCAGATAT CTTAGTAGGA GAAAACGACC TCACTGCCCT CAGCCACCTC 
CACGAGCCCG CAGTTCTGCA TAACTTAAAG GTCCGTTTCC TGGAATCCAA CCACATCTAC 
ACTTACTGTG GAATTGTGCT TGTTGCCATC AACCCCTACG AGCAGCTGCC AATCTACGGA 
CAGGATGTCA TCTATGCCTA CAGTGGCCAA AACATGGGTG ACATGGACCC CCACATCTTT 
GCTGTGGCAG AAGAAGCCTA CAAGCAGATG GCCAGAGATG AAAAGAACCA ATCCATCATA 
GTCAGCGGAG AGTCTGGAGC AGGCAAGACG GTGTCGGCCA AGTACGCCAT GCGCTATTTT 
GCCACCGTTG GTGGCTCAGC CAGTGATACC AACATTGAAG AGAAGGTCCT AGCATCCAGC 
CCCATCATGG AGGCCATTGG GAACGCCAAG ACTACTCGCA ACGACAACAG CAGCCGATTT 
GGGAAATTCA TTGAGATTGG CTTTGATAAA AAGTACCACA TCATTGGGGC CAACATGAGG 
ACCTACTTGC TGGAGAAGTC CAGGGTGGTC TTTCAGGCGG ATGACGAGAG GAACTACCAC 
ATCTTTTACC AGCTCTGTGC TGCTGCCAGC CTTCCTGAAT TTAAAGAGCT CGCCCTAACG 
TGCGCAGAGG ACTTTTTCTA TACAGCCCAT GGAGGAAACA CGACCATCGA GGGTGTGAAT 
GATGCAGACG ACTTTGAGAA GACTAGACAA GCCCTCACAC TCCTGGGAGT GCGGGATTCC 
CATCAAATAA GCATCTTTAA GATAATTGCT TCTATCTTGC ACCTGGGAAG TGTGGAGATT 
CAGTCTGAGC GGGACGGGGA TTCCTGCAGC ATTTCACCCC AGGATGAACA CTTGAGCAAC 
TTCTGCAGCC TGCTAGGAAT AGAACACAGC CAGATGGAGC ACTGGCTGTG TCATCGGAAG 
CTGGTCACCA CCTCAGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAAGT GGTCAATGCA 
CGTGATGCCT TAGCCAAGCA CATCTATGCT CAGCTGTTCT CCTGGATTGT GGAGCACATC 
AACAAAGCCC TGCACACCTC CCTTAAGCAG CACTCCTTCA TCGGGGTCCT GGATATCTAT 
GGGTTTGAGA CCTTCGAGAT CAATAGCTTT GAACAGTTTT GTATCAACTA CGCCAATGAA 
AAGCTCCAGC AACAGTTCAA TTCGCATGTG TTCAAGCTGG AGCAAGAAGA ATATATGAAG 
GAGCAGATCC CGTGGACTTT GATTGACTTC TACGATAACC AACCGTGTAT AGACCTCATA 
GAGGCAAAGC TGGGCATCCT GGACCTGTTG GATGAGGAGT GTAAGGTCCC CAAAGGAACT 
GATCAGAACT GGGCCCAGAA ACTCTACGAA CGACACTCCA ACAGCCAGCA CTTCCAGAAA 
CCACGCATGT CCAACACGGC CTTCATTGTC AACCACTTTG CAGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTAGAGAA AAACAGGGAT ACAGTATATG AAGAACAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCATT AGTGGCTGAT TTGTTCCACG ATGACAAGGA CTCTGCTCCA 
GCCACCAACA CAGCTAAGAA TCGATCATCT TCAAAGATCA ACGTTCGCTC TTCCAGACCC 
CTCATCAAAG TCCCTAACAA GGAGCACAAG AAATCCGTGG GCTACCAGTT TCGTACTTCC 
CTAAACCTGC TTATGGAGAC CCTGAATGCC ACAACGCCAC ACTATGTCCG GTGCATCAAG 
CCCAACGATG AAAAGCTTCC CTTCCACTTT GACCCCAAGA GAGCTGTACA GCAACTCCGA 
GCCTGCGGTG TGCTGGAGAC CATTCGGATC AGCGCGGCAG GCTACCCATC CAGGTGGACC 
TACCATGACT TCTTCAACCG GTATCGGGTG TTGATGAAGA AGAGAGAGCT CACCAACACC 
GACAAGAAGA ATATCTGCAA GTCTGTCCTG GAGAGTCTCA TCAAGGACCC AGACAAGTTC 
CAGTTTGGCC GCACCAAGAT CTTCTTCCGA GCAGGCCAGG TGGCCTACCT GGAGAAGCTT 
CGGGCAGACA AGTTCCGGGA AGCTACTATC ATGATCCAGA AGTCGGTCAG GGGATGGCTG 
CAGAGAGTGA AGTACCGTAG GCTGAGGGCA GCTACCTTAT CCCTGCAGAG GTTCTGCAGG 
GGATACCTAG CCCGCAGGCT GGCCGAACAC TTGAGGAGAA CCCGGGCCGC CATCGTGTTC 
CAGAAGCAGT ACCGCATGCT GAAGGCCCGC CGAGCCTACC GCAGGGTCTG CAGGGCTACC 
GTCATCATCC AGTCCTTCAC ACGGGCCATG TTTGTGCGCA GGAACTACCG CCAGGTCCTC 
ATGGAGCACA AAGCCACCAT CATCCAGAAG TACGCCCGAG GCTGGATGGC ACGGAAGCGT 
TTTCTGCGGG AGCGGGATGC AGCCATTGTC ATCCAGTGTG CCTTCCGGAG ACTCAAGGCC 
AGGCAGGAGC TGAAGGCCCT CAAGATTGAG GCCCGCTCGG CAGAGCATCT GAAACGCCTC 
AACGTGGGCA TGGAGAACAA AGTCGTCCAG CTACAGCGGA AGATTGATGA CCAGAACAAA 
GAGTTCAAGA CTCTGTCAGA GCAGCTGTCT GCAGTCACCT CCAGTCATGC CGTGGAGGTG 
GAGAAGCTGA AGAAGGAGCT GGCACATTAC CAGCAGAACC AGGAGGCTGA TACCAGCCTT 
CAGCTGCAGG AGGAGGTTCA GAGCCTGCGC ACTGAACTAC AGAAGGCTCA TTCGGAGCGC 
AGGGTCCTGG AGGATGCTCA CAACAAGGAG AATGGCGAGC TGAGAAAGCG AGTTGCAGAC 
CTGGAACATG AAAATGCCCT CTTGAAGGAT GAAAAAGAAT ACCTTAACAA CCAAATCCTG 
TGCCAGTCAA AAGCTGAATC TTCGCAGAGC TCTGTGGAAG AGAACCTCCT GATGAAGAAG 
GAGCTGGAGG AGGAACGGTC CAGGTACCAG AACCTCGTGA AGGAGTACTC CCAGCTGGAG 
CAGAGATATG AGAACCTTCG GGACGAGGTG ACGATCCTAA AGCAAACTCC GGGCCACAGG 
AAGAACCCAT CGAACCAAAG CAGCTTAGAA TCTGACTCCA ATTACCCCTC CATTTCCACT 
TCCGAAATCG GAGACACTGA GGATGCCCTT CAGCAGGTGG AGGAGATTGG CATAGAGAAG 
GCAGCCATGG ACATGACCGT CTTCCTGAAG CTGCAGAAGA GAGTGCGCGA ACTTGAGCAG 
GAGAGGAAGA AGCTGCAGGC ACAGCTAGAA AAGGGACAGC AGGACAGCAA GAAAGGGCAG 
GTAGAACAAC AGAACAATGG CTTAGATGTG GACCAGGACG CAGATATAGC CTACAATAGT 
CTGAAGAGAC AGGAGCTTGA GTCAGAGAAC AAGAAGCTGA AGAATGACCT GAATGAGCTG 
AGGAAGGCTG TCGCTGACCA AGCCATGCAG GATAACTCCA CCCACAGCTC CCCAGACAGC 
TACAGCCTCC TCCTGAACCA GCTCAAGCTG GCCAATGAGG AGCTCGAGGT CCGCAAAGAG 
GAGGTGCTGA TCCTCAGGAC CCAGATCATG AATGCCGACC AGCGCCGCCT GTCTGGCAAG 
AACATGGAGC CGAACATCAA TGCCAGAACA AGTTGGCCCA ACAGTGAGAA GCACGTGGAC 
CAGGAAGACG CCATTGAGGC CTATCACGGG GTCTGCCAGA CAAACAGCCA GACTGAGGAT 
TGGGGATATT TGAATGAAGA TGGAGAACTC GGCTTGGCTT ACCAAGGCCT AAAGCAAGTT 
GCCAGGTTGC TGGAGGCCCA GCTGCAGGCC CAGAGCCTGG AGCATGAGGA GGAGGTGGAA 
CATCTCAAGG CCCAGGTGGA AGCCCTGAAA GAGGAGATGG ACAAACAGCA GCAGACCTTC 
TGCCAGACCC TGCTGCTCTC CCCAGAGGCC CAGGTAGAAT TTGGTGTCCA GCAGGAGATA 
TCCCGGCTGA CCAATGAGAA CCTGGATTTT AAGGAATTGG TGGAAAAGCT GGAGAAGAAT 
GAGAGGAAGC TGAAGAAGCA GCTGAAGATT TACATGAAGA AGGTCCAGGA CTTAGAAGCT 
GCTCAGGCGT TGGCACAGAG TGACAGGAGA CACCATGAAC TCACAAGACA GGTCACAGTC 
CAACGAAAAG AGAAGGACTT CCAAGGCATG CTGGAGTACC ACAAAGAGGA CGAAGCCCTC 
CTCATCCGGA ACCTGGTGAC AGACCTGAAG CCTCAGATGC TGTCGGGCAC CGTGCCCTGT 
CTGCCTGCAT ACATACTCTA TATGTGCATC AGGCACGCGG ATTACACCAA CGATGACCTC 
AAGGTGCACT CGTTGCTGAG CTCCACCATC AACGGCATTA AGAAAGTCCT CAAGAAGCAC 
AATGATGACT TTGAGATGAC GTCATTCTGG CTATCCAACA CCTGCCGCTT CCTTCACTGT 
CTGAAGCAAT ACAGTGGTGA TGAGGGTTTC ATGACACAGA ACACTGCGAA GCAGAATGAG 
CACTGTCTCA AGAACTTTGA CCTCACTGAA TACCGCCAGG TGCTAAGCGA CCTTTCCATT 
CAGATCTATC AGCAGCTCAT TAAAATCGCC GAGGGCTTGC TGCAGCCTAT GATAGTTTCT 
GCCATGTTGG AAAATGAGAG TATCCAGGGG CTGTCTGGTG TGAGACCAAC TGGTTACCGG 
AAGCGCTCCT CCAGCATGGT GGATGGAGAG AATTCGTATT GCCTGGAGGC CATCGTCCGC 
CAGATGAATT CTTTCCATAC AGTCCTGTGT GACCAGGGTC TGGACCCCGA GATTATCCTG 
CAGGTGTTCA AACAGCTCTT CTACATGATC AACGCTGTGA CTCTTAACAA CCTACTCCTG 
CGGAAAGACG CCTGCTCCTG GAGCACAGGC ATGCAACTCA GGTACAACAT AAGTCAACTG 
GAAGAGTGGC TTCGGGGCAA AAACCTTCAC CAAAGTGGAG CAGTTCAGAC CATGGAGCCC 
CTCATCCAGG CAGCCCAGCT CCTCCAGCTG AAGAAGAAAA CCCACGAGGA TGCTGAGGCC 
ATCTGCTCTC TGTGCACCTC CCTCAGCACC CAGCAGATTG TCAAAATTTT AAACCTCTAC 
ACTCCCTTGA ATGAATTTGA GGAACGGGTC ACAGTGTCCT TCATACGAAC AATCCAGGCT 
CAGCTACAAG AGAGGAATGA CCCTCAGCAG CTCCTGCTGG ACTCCAAGCA CGTGTTCCCA 
GTTCTGTTTC CATATAACCC ATCTGCTCTG ACCATGGACT CGATCCACAT CCCGGCCTGT 
CTCAACCTGG AGTTTCTCAA TGAAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525777.1
CDS	418..5967
Translation	MSYSELYTRYTRVWIPDPDEVWRSAELTKDYKEGDKSLQLRLEDDTILEYPVDVQNNQVPFLRNPDILVGENDLTALSHLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKFIEIGFDKKYHIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTAHGGNTTIEGVNDADDFEKTRQALTLLGVRDSHQISIFKIIASILHLGSVEIQSERDGDSCSISPQDEHLSNFCSLLGIEHSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARDALAKHIYAQLFSWIVEHINKALHTSLKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYERHSNSQHFQKPRMSNTAFIVNHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDDKDSAPATNTAKNRSSSKINVRSSRPLIKVPNKEHKKSVGYQFRTSLNLLMETLNATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWTYHDFFNRYRVLMKKRELTNTDKKNICKSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFREATIMIQKSVRGWLQRVKYRRLRAATLSLQRFCRGYLARRLAEHLRRTRAAIVFQKQYRMLKARRAYRRVCRATVIIQSFTRAMFVRRNYRQVLMEHKATIIQKYARGWMARKRFLRERDAAIVIQCAFRRLKARQELKALKIEARSAEHLKRLNVGMENKVVQLQRKIDDQNKEFKTLSEQLSAVTSSHAVEVEKLKKELAHYQQNQEADTSLQLQEEVQSLRTELQKAHSERRVLEDAHNKENGELRKRVADLEHENALLKDEKEYLNNQILCQSKAESSQSSVEENLLMKKELEEERSRYQNLVKEYSQLEQRYENLRDEVTILKQTPGHRKNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGIEKAAMDMTVFLKLQKRVRELEQERKKLQAQLEKGQQDSKKGQVEQQNNGLDVDQDADIAYNSLKRQELESENKKLKNDLNELRKAVADQAMQDNSTHSSPDSYSLLLNQLKLANEELEVRKEEVLILRTQIMNADQRRLSGKNMEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNSQTEDWGYLNEDGELGLAYQGLKQVARLLEAQLQAQSLEHEEEVEHLKAQVEALKEEMDKQQQTFCQTLLLSPEAQVEFGVQQEISRLTNENLDFKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSDRRHHELTRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTDLKPQMLSGTVPCLPAYILYMCIRHADYTNDDLKVHSLLSSTINGIKKVLKKHNDDFEMTSFWLSNTCRFLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGLLQPMIVSAMLENESIQGLSGVRPTGYRKRSSSMVDGENSYCLEAIVRQMNSFHTVLCDQGLDPEIILQVFKQLFYMINAVTLNNLLLRKDACSWSTGMQLRYNISQLEEWLRGKNLHQSGAVQTMEPLIQAAQLLQLKKKTHEDAEAICSLCTSLSTQQIVKILNLYTPLNEFEERVTVSFIRTIQAQLQERNDPQQLLLDSKHVFPVLFPYNPSALTMDSIHIPACLNLEFLNEV

Target ORF information:

RefSeq Version	XM_006525714.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VB (Myo5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525714.4

ORF Insert Sequence:

ATGTCGTACA GCGAGCTCTA CACCCGGTAC ACAAGGGTCT GGATCCCTGA CCCAGATGAG 
GTGTGGCGCT CGGCTGAATT AACCAAGGAC TACAAAGAAG GGGATAAGAG CCTGCAGCTC 
AGACTAGAAG ATGACACAAT TCTAGAATAC CCAGTTGATG TCCAAAATAA CCAGGTGCCA 
TTCTTGCGGA ATCCAGATAT CTTAGTAGGA GAAAACGACC TCACTGCCCT CAGCCACCTC 
CACGAGCCCG CAGTTCTGCA TAACTTAAAG GTCCGTTTCC TGGAATCCAA CCACATCTAC 
ACTTACTGTG GAATTGTGCT TGTTGCCATC AACCCCTACG AGCAGCTGCC AATCTACGGA 
CAGGATGTCA TCTATGCCTA CAGTGGCCAA AACATGGGTG ACATGGACCC CCACATCTTT 
GCTGTGGCAG AAGAAGCCTA CAAGCAGATG GCCAGAGATG AAAAGAACCA ATCCATCATA 
GTCAGCGGAG AGTCTGGAGC AGGCAAGACG GTGTCGGCCA AGTACGCCAT GCGCTATTTT 
GCCACCGTTG GTGGCTCAGC CAGTGATACC AACATTGAAG AGAAGGTCCT AGCATCCAGC 
CCCATCATGG AGGCCATTGG GAACGCCAAG ACTACTCGCA ACGACAACAG CAGCCGATTT 
GGGAAATTCA TTGAGATTGG CTTTGATAAA AAGTACCACA TCATTGGGGC CAACATGAGG 
ACCTACTTGC TGGAGAAGTC CAGGGTGGTC TTTCAGGCGG ATGACGAGAG GAACTACCAC 
ATCTTTTACC AGCTCTGTGC TGCTGCCAGC CTTCCTGAAT TTAAAGAGCT CGCCCTAACG 
TGCGCAGAGG ACTTTTTCTA TACAGCCCAT GGAGGAAACA CGACCATCGA GGGTGTGAAT 
GATGCAGACG ACTTTGAGAA GACTAGACAA GCCCTCACAC TCCTGGGAGT GCGGGATTCC 
CATCAAATAA GCATCTTTAA GATAATTGCT TCTATCTTGC ACCTGGGAAG TGTGGAGATT 
CAGTCTGAGC GGGACGGGGA TTCCTGCAGC ATTTCACCCC AGGATGAACA CTTGAGCAAC 
TTCTGCAGCC TGCTAGGAAT AGAACACAGC CAGATGGAGC ACTGGCTGTG TCATCGGAAG 
CTGGTCACCA CCTCAGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAAGT GGTCAATGCA 
CGTGATGCCT TAGCCAAGCA CATCTATGCT CAGCTGTTCT CCTGGATTGT GGAGCACATC 
AACAAAGCCC TGCACACCTC CCTTAAGCAG CACTCCTTCA TCGGGGTCCT GGATATCTAT 
GGGTTTGAGA CCTTCGAGAT CAATAGCTTT GAACAGTTTT GTATCAACTA CGCCAATGAA 
AAGCTCCAGC AACAGTTCAA TTCGCATGTG TTCAAGCTGG AGCAAGAAGA ATATATGAAG 
GAGCAGATCC CGTGGACTTT GATTGACTTC TACGATAACC AACCGTGTAT AGACCTCATA 
GAGGCAAAGC TGGGCATCCT GGACCTGTTG GATGAGGAGT GTAAGGTCCC CAAAGGAACT 
GATCAGAACT GGGCCCAGAA ACTCTACGAA CGACACTCCA ACAGCCAGCA CTTCCAGAAA 
CCACGCATGT CCAACACGGC CTTCATTGTC AACCACTTTG CAGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTAGAGAA AAACAGGGAT ACAGTATATG AAGAACAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCATT AGTGGCTGAT TTGTTCCACG ATGACAAGGA CTCTGCTCCA 
GCCACCAACA CAGCTAAGAA TCGATCATCT TCAAAGATCA ACGTTCGCTC TTCCAGACCC 
CTCATCAAAG TCCCTAACAA GGAGCACAAG AAATCCGTGG GCTACCAGTT TCGTACTTCC 
CTAAACCTGC TTATGGAGAC CCTGAATGCC ACAACGCCAC ACTATGTCCG GTGCATCAAG 
CCCAACGATG AAAAGCTTCC CTTCCACTTT GACCCCAAGA GAGCTGTACA GCAACTCCGA 
GCCTGCGGTG TGCTGGAGAC CATTCGGATC AGCGCGGCAG GCTACCCATC CAGGTGGACC 
TACCATGACT TCTTCAACCG GTATCGGGTG TTGATGAAGA AGAGAGAGCT CACCAACACC 
GACAAGAAGA ATATCTGCAA GTCTGTCCTG GAGAGTCTCA TCAAGGACCC AGACAAGTTC 
CAGTTTGGCC GCACCAAGAT CTTCTTCCGA GCAGGCCAGG TGGCCTACCT GGAGAAGCTT 
CGGGCAGACA AGTTCCGGGA AGCTACTATC ATGATCCAGA AGTCGGTCAG GGGATGGCTG 
CAGAGAGTGA AGTACCGTAG GCTGAGGGCA GCTACCTTAT CCCTGCAGAG GTTCTGCAGG 
GGATACCTAG CCCGCAGGCT GGCCGAACAC TTGAGGAGAA CCCGGGCCGC CATCGTGTTC 
CAGAAGCAGT ACCGCATGCT GAAGGCCCGC CGAGCCTACC GCAGGGTCTG CAGGGCTACC 
GTCATCATCC AGTCCTTCAC ACGGGCCATG TTTGTGCGCA GGAACTACCG CCAGGTCCTC 
ATGGAGCACA AAGCCACCAT CATCCAGAAG TACGCCCGAG GCTGGATGGC ACGGAAGCGT 
TTTCTGCGGG AGCGGGATGC AGCCATTGTC ATCCAGTGTG CCTTCCGGAG ACTCAAGGCC 
AGGCAGGAGC TGAAGGCCCT CAAGATTGAG GCCCGCTCGG CAGAGCATCT GAAACGCCTC 
AACGTGGGCA TGGAGAACAA AGTCGTCCAG CTACAGCGGA AGATTGATGA CCAGAACAAA 
GAGTTCAAGA CTCTGTCAGA GCAGCTGTCT GCAGTCACCT CCAGTCATGC CGTGGAGGTG 
GAGAAGCTGA AGAAGGAGCT GGCACATTAC CAGCAGAACC AGGAGGCTGA TACCAGCCTT 
CAGCTGCAGG AGGAGGTTCA GAGCCTGCGC ACTGAACTAC AGAAGGCTCA TTCGGAGCGC 
AGGGTCCTGG AGGATGCTCA CAACAAGGAG AATGGCGAGC TGAGAAAGCG AGTTGCAGAC 
CTGGAACATG AAAATGCCCT CTTGAAGGAT GAAAAAGAAT ACCTTAACAA CCAAATCCTG 
TGCCAGTCAA AAGCTGAATC TTCGCAGAGC TCTGTGGAAG AGAACCTCCT GATGAAGAAG 
GAGCTGGAGG AGGAACGGTC CAGGTACCAG AACCTCGTGA AGGAGTACTC CCAGCTGGAG 
CAGAGATATG AGAACCTTCG GGACGAGGTG ACGATCCTAA AGCAAACTCC GGGCCACAGG 
AAGAACCCAT CGAACCAAAG CAGCTTAGAA TCTGACTCCA ATTACCCCTC CATTTCCACT 
TCCGAAATCG GAGACACTGA GGATGCCCTT CAGCAGGTGG AGGAGATTGG CATAGAGAAG 
GCAGCCATGG ACATGACCGT CTTCCTGAAG CTGCAGAAGA GAGTGCGCGA ACTTGAGCAG 
GAGAGGAAGA AGCTGCAGGC ACAGCTAGAA AAGGGACAGC AGGACAGCAA GAAAGGGCAG 
GTAGAACAAC AGAACAATGG CTTAGATGTG GACCAGGACG CAGATATAGC CTACAATAGT 
CTGAAGAGAC AGGAGCTTGA GTCAGAGAAC AAGAAGCTGA AGAATGACCT GAATGAGCTG 
AGGAAGGCTG TCGCTGACCA AGCCATGCAG GATAACTCCA CCCACAGCTC CCCAGACAGC 
TACAGCCTCC TCCTGAACCA GCTCAAGCTG GCCAATGAGG AGCTCGAGGT CCGCAAAGAG 
GAGGTGCTGA TCCTCAGGAC CCAGATCATG AATGCCGACC AGCGCCGCCT GTCTGGCAAG 
AACATGGAGC CGAACATCAA TGCCAGAACA AGTTGGCCCA ACAGTGAGAA GCACGTGGAC 
CAGGAAGACG CCATTGAGGC CTATCACGGG GTCTGCCAGA CAAACAGCCA GACTGAGGAT 
TGGGGATATT TGAATGAAGA TGGAGAACTC GGCTTGGCTT ACCAAGGCCT AAAGCAAGTT 
GCCAGGTTGC TGGAGGCCCA GCTGCAGGCC CAGAGCCTGG AGCATGAGGA GGAGGTGGAA 
CATCTCAAGG CCCAGGTGGA AGCCCTGAAA GAGGAGATGG ACAAACAGCA GCAGACCTTC 
TGCCAGACCC TGCTGCTCTC CCCAGAGGCC CAGGTAGAAT TTGGTGTCCA GCAGGAGATA 
TCCCGGCTGA CCAATGAGAA CCTGGATTTT AAGGAATTGG TGGAAAAGCT GGAGAAGAAT 
GAGAGGAAGC TGAAGAAGCA GCTGAAGATT TACATGAAGA AGGTCCAGGA CTTAGAAGCT 
GCTCAGGCGT TGGCACAGAG TGACAGGAGA CACCATGAAC TCACAAGACA GGTCACAGTC 
CAACGAAAAG AGAAGGACTT CCAAGGCATG CTGGAGTACC ACAAAGAGGA CGAAGCCCTC 
CTCATCCGGA ACCTGGTGAC AGACCTGAAG CCTCAGATGC TGTCGGGCAC CGTGCCCTGT 
CTGCCTGCAT ACATACTCTA TATGTGCATC AGGCACGCGG ATTACACCAA CGATGACCTC 
AAGGTGCACT CGTTGCTGAG CTCCACCATC AACGGCATTA AGAAAGTCCT CAAGAAGCAC 
AATGATGACT TTGAGATGAC GTCATTCTGG CTATCCAACA CCTGCCGCTT CCTTCACTGT 
CTGAAGCAAT ACAGTGGTGA TGAGGGTTTC ATGACACAGA ACACTGCGAA GCAGAATGAG 
CACTGTCTCA AGAACTTTGA CCTCACTGAA TACCGCCAGG TGCTAAGCGA CCTTTCCATT 
CAGATCTATC AGCAGCTCAT TAAAATCGCC GAGGGCTTGC TGCAGCCTAT GATAGTTTCT 
GCCATGTTGG AAAATGAGAG TATCCAGGGG CTGTCTGGTG TGAGACCAAC TGGTTACCGG 
AAGCGCTCCT CCAGCATGGT GGATGGAGAG AATTCGTATT GCCTGGAGGC CATCGTCCGC 
CAGATGAATT CTTTCCATAC AGTCCTGTGT GACCAGGGTC TGGACCCCGA GATTATCCTG 
CAGGTGTTCA AACAGCTCTT CTACATGATC AACGCTGTGA CTCTTAACAA CCTACTCCTG 
CGGAAAGACG CCTGCTCCTG GAGCACAGGC ATGCAACTCA GGTACAACAT AAGTCAACTG 
GAAGAGTGGC TTCGGGGCAA AAACCTTCAC CAAAGTGGAG CAGTTCAGAC CATGGAGCCC 
CTCATCCAGG CAGCCCAGCT CCTCCAGCTG AAGAAGAAAA CCCACGAGGA TGCTGAGGCC 
ATCTGCTCTC TGTGCACCTC CCTCAGCACC CAGCAGATTG TCAAAATTTT AAACCTCTAC 
ACTCCCTTGA ATGAATTTGA GGAACGGGTC ACAGTGTCCT TCATACGAAC AATCCAGGCT 
CAGCTACAAG AGAGGAATGA CCCTCAGCAG CTCCTGCTGG ACTCCAAGCA CGTGTTCCCA 
GTTCTGTTTC CATATAACCC ATCTGCTCTG ACCATGGACT CGATCCACAT CCCGGCCTGT 
CTCAACCTGG AGTTTCTCAA TGAAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5b ( XM_006525715.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525778.1 Mus musculus myosin VB (Myo5b), transcript variant X2, mRNA.

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CloneID	OMu54431
Clone ID Related Accession (Same CDS sequence)	XM_006525715.3 , XM_006525715.4
Accession Version	XM_006525715.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Vb isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525715.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAA GAGGTAGTAG GTCCTCTCTG CTGCACAATA GTACATGGCA GTACACAAGG 
GTCTGGATCC CTGACCCAGA TGAGGTGTGG CGCTCGGCTG AATTAACCAA GGACTACAAA 
GAAGGGGATA AGAGCCTGCA GCTCAGACTA GAAGATGACA CAATTCTAGA ATACCCAGTT 
GATGTCCAAA ATAACCAGGT GCCATTCTTG CGGAATCCAG ATATCTTAGT AGGAGAAAAC 
GACCTCACTG CCCTCAGCCA CCTCCACGAG CCCGCAGTTC TGCATAACTT AAAGGTCCGT 
TTCCTGGAAT CCAACCACAT CTACACTTAC TGTGGAATTG TGCTTGTTGC CATCAACCCC 
TACGAGCAGC TGCCAATCTA CGGACAGGAT GTCATCTATG CCTACAGTGG CCAAAACATG 
GGTGACATGG ACCCCCACAT CTTTGCTGTG GCAGAAGAAG CCTACAAGCA GATGGCCAGA 
GATGAAAAGA ACCAATCCAT CATAGTCAGC GGAGAGTCTG GAGCAGGCAA GACGGTGTCG 
GCCAAGTACG CCATGCGCTA TTTTGCCACC GTTGGTGGCT CAGCCAGTGA TACCAACATT 
GAAGAGAAGG TCCTAGCATC CAGCCCCATC ATGGAGGCCA TTGGGAACGC CAAGACTACT 
CGCAACGACA ACAGCAGCCG ATTTGGGAAA TTCATTGAGA TTGGCTTTGA TAAAAAGTAC 
CACATCATTG GGGCCAACAT GAGGACCTAC TTGCTGGAGA AGTCCAGGGT GGTCTTTCAG 
GCGGATGACG AGAGGAACTA CCACATCTTT TACCAGCTCT GTGCTGCTGC CAGCCTTCCT 
GAATTTAAAG AGCTCGCCCT AACGTGCGCA GAGGACTTTT TCTATACAGC CCATGGAGGA 
AACACGACCA TCGAGGGTGT GAATGATGCA GACGACTTTG AGAAGACTAG ACAAGCCCTC 
ACACTCCTGG GAGTGCGGGA TTCCCATCAA ATAAGCATCT TTAAGATAAT TGCTTCTATC 
TTGCACCTGG GAAGTGTGGA GATTCAGTCT GAGCGGGACG GGGATTCCTG CAGCATTTCA 
CCCCAGGATG AACACTTGAG CAACTTCTGC AGCCTGCTAG GAATAGAACA CAGCCAGATG 
GAGCACTGGC TGTGTCATCG GAAGCTGGTC ACCACCTCAG AGACCTACGT CAAGACCATG 
TCCCTGCAGC AAGTGGTCAA TGCACGTGAT GCCTTAGCCA AGCACATCTA TGCTCAGCTG 
TTCTCCTGGA TTGTGGAGCA CATCAACAAA GCCCTGCACA CCTCCCTTAA GCAGCACTCC 
TTCATCGGGG TCCTGGATAT CTATGGGTTT GAGACCTTCG AGATCAATAG CTTTGAACAG 
TTTTGTATCA ACTACGCCAA TGAAAAGCTC CAGCAACAGT TCAATTCGCA TGTGTTCAAG 
CTGGAGCAAG AAGAATATAT GAAGGAGCAG ATCCCGTGGA CTTTGATTGA CTTCTACGAT 
AACCAACCGT GTATAGACCT CATAGAGGCA AAGCTGGGCA TCCTGGACCT GTTGGATGAG 
GAGTGTAAGG TCCCCAAAGG AACTGATCAG AACTGGGCCC AGAAACTCTA CGAACGACAC 
TCCAACAGCC AGCACTTCCA GAAACCACGC ATGTCCAACA CGGCCTTCAT TGTCAACCAC 
TTTGCAGATA AGGTGGAGTA CCTTTCAGAT GGTTTTCTAG AGAAAAACAG GGATACAGTA 
TATGAAGAAC AGATCAACAT CCTGAAGGCC AGCAAGTTCC CATTAGTGGC TGATTTGTTC 
CACGATGACA AGGACTCTGC TCCAGCCACC AACACAGCTA AGAATCGATC ATCTTCAAAG 
ATCAACGTTC GCTCTTCCAG ACCCCTCATC AAAGTCCCTA ACAAGGAGCA CAAGAAATCC 
GTGGGCTACC AGTTTCGTAC TTCCCTAAAC CTGCTTATGG AGACCCTGAA TGCCACAACG 
CCACACTATG TCCGGTGCAT CAAGCCCAAC GATGAAAAGC TTCCCTTCCA CTTTGACCCC 
AAGAGAGCTG TACAGCAACT CCGAGCCTGC GGTGTGCTGG AGACCATTCG GATCAGCGCG 
GCAGGCTACC CATCCAGGTG GACCTACCAT GACTTCTTCA ACCGGTATCG GGTGTTGATG 
AAGAAGAGAG AGCTCACCAA CACCGACAAG AAGAATATCT GCAAGTCTGT CCTGGAGAGT 
CTCATCAAGG ACCCAGACAA GTTCCAGTTT GGCCGCACCA AGATCTTCTT CCGAGCAGGC 
CAGGTGGCCT ACCTGGAGAA GCTTCGGGCA GACAAGTTCC GGGAAGCTAC TATCATGATC 
CAGAAGTCGG TCAGGGGATG GCTGCAGAGA GTGAAGTACC GTAGGCTGAG GGCAGCTACC 
TTATCCCTGC AGAGGTTCTG CAGGGGATAC CTAGCCCGCA GGCTGGCCGA ACACTTGAGG 
AGAACCCGGG CCGCCATCGT GTTCCAGAAG CAGTACCGCA TGCTGAAGGC CCGCCGAGCC 
TACCGCAGGG TCTGCAGGGC TACCGTCATC ATCCAGTCCT TCACACGGGC CATGTTTGTG 
CGCAGGAACT ACCGCCAGGT CCTCATGGAG CACAAAGCCA CCATCATCCA GAAGTACGCC 
CGAGGCTGGA TGGCACGGAA GCGTTTTCTG CGGGAGCGGG ATGCAGCCAT TGTCATCCAG 
TGTGCCTTCC GGAGACTCAA GGCCAGGCAG GAGCTGAAGG CCCTCAAGAT TGAGGCCCGC 
TCGGCAGAGC ATCTGAAACG CCTCAACGTG GGCATGGAGA ACAAAGTCGT CCAGCTACAG 
CGGAAGATTG ATGACCAGAA CAAAGAGTTC AAGACTCTGT CAGAGCAGCT GTCTGCAGTC 
ACCTCCAGTC ATGCCGTGGA GGTGGAGAAG CTGAAGAAGG AGCTGGCACA TTACCAGCAG 
AACCAGGAGG CTGATACCAG CCTTCAGCTG CAGGAGGAGG TTCAGAGCCT GCGCACTGAA 
CTACAGAAGG CTCATTCGGA GCGCAGGGTC CTGGAGGATG CTCACAACAA GGAGAATGGC 
GAGCTGAGAA AGCGAGTTGC AGACCTGGAA CATGAAAATG CCCTCTTGAA GGATGAAAAA 
GAATACCTTA ACAACCAAAT CCTGTGCCAG TCAAAAGCTG AATCTTCGCA GAGCTCTGTG 
GAAGAGAACC TCCTGATGAA GAAGGAGCTG GAGGAGGAAC GGTCCAGGTA CCAGAACCTC 
GTGAAGGAGT ACTCCCAGCT GGAGCAGAGA TATGAGAACC TTCGGGACGA GGTGACGATC 
CTAAAGCAAA CTCCGGGCCA CAGGAAGAAC CCATCGAACC AAAGCAGCTT AGAATCTGAC 
TCCAATTACC CCTCCATTTC CACTTCCGAA ATCGGAGACA CTGAGGATGC CCTTCAGCAG 
GTGGAGGAGA TTGGCATAGA GAAGGCAGCC ATGGACATGA CCGTCTTCCT GAAGCTGCAG 
AAGAGAGTGC GCGAACTTGA GCAGGAGAGG AAGAAGCTGC AGGCACAGCT AGAAAAGGGA 
CAGCAGGACA GCAAGAAAGG GCAGGTAGAA CAACAGAACA ATGGCTTAGA TGTGGACCAG 
GACGCAGATA TAGCCTACAA TAGTCTGAAG AGACAGGAGC TTGAGTCAGA GAACAAGAAG 
CTGAAGAATG ACCTGAATGA GCTGAGGAAG GCTGTCGCTG ACCAAGCCAT GCAGGATAAC 
TCCACCCACA GCTCCCCAGA CAGCTACAGC CTCCTCCTGA ACCAGCTCAA GCTGGCCAAT 
GAGGAGCTCG AGGTCCGCAA AGAGGAGGTG CTGATCCTCA GGACCCAGAT CATGAATGCC 
GACCAGCGCC GCCTGTCTGG CAAGAACATG GAGCCGAACA TCAATGCCAG AACAAGTTGG 
CCCAACAGTG AGAAGCACGT GGACCAGGAA GACGCCATTG AGGCCTATCA CGGGGTCTGC 
CAGACAAACA GGTTGCTGGA GGCCCAGCTG CAGGCCCAGA GCCTGGAGCA TGAGGAGGAG 
GTGGAACATC TCAAGGCCCA GGTGGAAGCC CTGAAAGAGG AGATGGACAA ACAGCAGCAG 
ACCTTCTGCC AGACCCTGCT GCTCTCCCCA GAGGCCCAGG TAGAATTTGG TGTCCAGCAG 
GAGATATCCC GGCTGACCAA TGAGAACCTG GATTTTAAGG AATTGGTGGA AAAGCTGGAG 
AAGAATGAGA GGAAGCTGAA GAAGCAGCTG AAGATTTACA TGAAGAAGGT CCAGGACTTA 
GAAGCTGCTC AGGCGTTGGC ACAGAGTGAC AGGAGACACC ATGAACTCAC AAGACAGGTC 
ACAGTCCAAC GAAAAGAGAA GGACTTCCAA GGCATGCTGG AGTACCACAA AGAGGACGAA 
GCCCTCCTCA TCCGGAACCT GGTGACAGAC CTGAAGCCTC AGATGCTGTC GGGCACCGTG 
CCCTGTCTGC CTGCATACAT ACTCTATATG TGCATCAGGC ACGCGGATTA CACCAACGAT 
GACCTCAAGG TGCACTCGTT GCTGAGCTCC ACCATCAACG GCATTAAGAA AGTCCTCAAG 
AAGCACAATG ATGACTTTGA GATGACGTCA TTCTGGCTAT CCAACACCTG CCGCTTCCTT 
CACTGTCTGA AGCAATACAG TGGTGATGAG GGTTTCATGA CACAGAACAC TGCGAAGCAG 
AATGAGCACT GTCTCAAGAA CTTTGACCTC ACTGAATACC GCCAGGTGCT AAGCGACCTT 
TCCATTCAGA TCTATCAGCA GCTCATTAAA ATCGCCGAGG GCTTGCTGCA GCCTATGATA 
GTTTCTGCCA TGTTGGAAAA TGAGAGTATC CAGGGGCTGT CTGGTGTGAG ACCAACTGGT 
TACCGGAAGC GCTCCTCCAG CATGGTGGAT GGAGAGAATT CGTATTGCCT GGAGGCCATC 
GTCCGCCAGA TGAATTCTTT CCATACAGTC CTGTGTGACC AGGGTCTGGA CCCCGAGATT 
ATCCTGCAGG TGTTCAAACA GCTCTTCTAC ATGATCAACG CTGTGACTCT TAACAACCTA 
CTCCTGCGGA AAGACGCCTG CTCCTGGAGC ACAGGCATGC AACTCAGGTA CAACATAAGT 
CAACTGGAAG AGTGGCTTCG GGGCAAAAAC CTTCACCAAA GTGGAGCAGT TCAGACCATG 
GAGCCCCTCA TCCAGGCAGC CCAGCTCCTC CAGCTGAAGA AGAAAACCCA CGAGGATGCT 
GAGGCCATCT GCTCTCTGTG CACCTCCCTC AGCACCCAGC AGATTGTCAA AATTTTAAAC 
CTCTACACTC CCTTGAATGA ATTTGAGGAA CGGGTCACAG TGTCCTTCAT ACGAACAATC 
CAGGCTCAGC TACAAGAGAG GAATGACCCT CAGCAGCTCC TGCTGGACTC CAAGCACGTG 
TTCCCAGTTC TGTTTCCATA TAACCCATCT GCTCTGACCA TGGACTCGAT CCACATCCCG 
GCCTGTCTCA ACCTGGAGTT TCTCAATGAA GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525778.1
CDS	96..5591
Translation	MDPRGSRSSLLHNSTWQYTRVWIPDPDEVWRSAELTKDYKEGDKSLQLRLEDDTILEYPVDVQNNQVPFLRNPDILVGENDLTALSHLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKFIEIGFDKKYHIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTAHGGNTTIEGVNDADDFEKTRQALTLLGVRDSHQISIFKIIASILHLGSVEIQSERDGDSCSISPQDEHLSNFCSLLGIEHSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARDALAKHIYAQLFSWIVEHINKALHTSLKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYERHSNSQHFQKPRMSNTAFIVNHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDDKDSAPATNTAKNRSSSKINVRSSRPLIKVPNKEHKKSVGYQFRTSLNLLMETLNATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWTYHDFFNRYRVLMKKRELTNTDKKNICKSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFREATIMIQKSVRGWLQRVKYRRLRAATLSLQRFCRGYLARRLAEHLRRTRAAIVFQKQYRMLKARRAYRRVCRATVIIQSFTRAMFVRRNYRQVLMEHKATIIQKYARGWMARKRFLRERDAAIVIQCAFRRLKARQELKALKIEARSAEHLKRLNVGMENKVVQLQRKIDDQNKEFKTLSEQLSAVTSSHAVEVEKLKKELAHYQQNQEADTSLQLQEEVQSLRTELQKAHSERRVLEDAHNKENGELRKRVADLEHENALLKDEKEYLNNQILCQSKAESSQSSVEENLLMKKELEEERSRYQNLVKEYSQLEQRYENLRDEVTILKQTPGHRKNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGIEKAAMDMTVFLKLQKRVRELEQERKKLQAQLEKGQQDSKKGQVEQQNNGLDVDQDADIAYNSLKRQELESENKKLKNDLNELRKAVADQAMQDNSTHSSPDSYSLLLNQLKLANEELEVRKEEVLILRTQIMNADQRRLSGKNMEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNRLLEAQLQAQSLEHEEEVEHLKAQVEALKEEMDKQQQTFCQTLLLSPEAQVEFGVQQEISRLTNENLDFKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSDRRHHELTRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTDLKPQMLSGTVPCLPAYILYMCIRHADYTNDDLKVHSLLSSTINGIKKVLKKHNDDFEMTSFWLSNTCRFLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGLLQPMIVSAMLENESIQGLSGVRPTGYRKRSSSMVDGENSYCLEAIVRQMNSFHTVLCDQGLDPEIILQVFKQLFYMINAVTLNNLLLRKDACSWSTGMQLRYNISQLEEWLRGKNLHQSGAVQTMEPLIQAAQLLQLKKKTHEDAEAICSLCTSLSTQQIVKILNLYTPLNEFEERVTVSFIRTIQAQLQERNDPQQLLLDSKHVFPVLFPYNPSALTMDSIHIPACLNLEFLNEV

Target ORF information:

RefSeq Version	XM_006525715.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VB (Myo5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525715.4

ORF Insert Sequence:

ATGGATCCAA GAGGTAGTAG GTCCTCTCTG CTGCACAATA GTACATGGCA GTACACAAGG 
GTCTGGATCC CTGACCCAGA TGAGGTGTGG CGCTCGGCTG AATTAACCAA GGACTACAAA 
GAAGGGGATA AGAGCCTGCA GCTCAGACTA GAAGATGACA CAATTCTAGA ATACCCAGTT 
GATGTCCAAA ATAACCAGGT GCCATTCTTG CGGAATCCAG ATATCTTAGT AGGAGAAAAC 
GACCTCACTG CCCTCAGCCA CCTCCACGAG CCCGCAGTTC TGCATAACTT AAAGGTCCGT 
TTCCTGGAAT CCAACCACAT CTACACTTAC TGTGGAATTG TGCTTGTTGC CATCAACCCC 
TACGAGCAGC TGCCAATCTA CGGACAGGAT GTCATCTATG CCTACAGTGG CCAAAACATG 
GGTGACATGG ACCCCCACAT CTTTGCTGTG GCAGAAGAAG CCTACAAGCA GATGGCCAGA 
GATGAAAAGA ACCAATCCAT CATAGTCAGC GGAGAGTCTG GAGCAGGCAA GACGGTGTCG 
GCCAAGTACG CCATGCGCTA TTTTGCCACC GTTGGTGGCT CAGCCAGTGA TACCAACATT 
GAAGAGAAGG TCCTAGCATC CAGCCCCATC ATGGAGGCCA TTGGGAACGC CAAGACTACT 
CGCAACGACA ACAGCAGCCG ATTTGGGAAA TTCATTGAGA TTGGCTTTGA TAAAAAGTAC 
CACATCATTG GGGCCAACAT GAGGACCTAC TTGCTGGAGA AGTCCAGGGT GGTCTTTCAG 
GCGGATGACG AGAGGAACTA CCACATCTTT TACCAGCTCT GTGCTGCTGC CAGCCTTCCT 
GAATTTAAAG AGCTCGCCCT AACGTGCGCA GAGGACTTTT TCTATACAGC CCATGGAGGA 
AACACGACCA TCGAGGGTGT GAATGATGCA GACGACTTTG AGAAGACTAG ACAAGCCCTC 
ACACTCCTGG GAGTGCGGGA TTCCCATCAA ATAAGCATCT TTAAGATAAT TGCTTCTATC 
TTGCACCTGG GAAGTGTGGA GATTCAGTCT GAGCGGGACG GGGATTCCTG CAGCATTTCA 
CCCCAGGATG AACACTTGAG CAACTTCTGC AGCCTGCTAG GAATAGAACA CAGCCAGATG 
GAGCACTGGC TGTGTCATCG GAAGCTGGTC ACCACCTCAG AGACCTACGT CAAGACCATG 
TCCCTGCAGC AAGTGGTCAA TGCACGTGAT GCCTTAGCCA AGCACATCTA TGCTCAGCTG 
TTCTCCTGGA TTGTGGAGCA CATCAACAAA GCCCTGCACA CCTCCCTTAA GCAGCACTCC 
TTCATCGGGG TCCTGGATAT CTATGGGTTT GAGACCTTCG AGATCAATAG CTTTGAACAG 
TTTTGTATCA ACTACGCCAA TGAAAAGCTC CAGCAACAGT TCAATTCGCA TGTGTTCAAG 
CTGGAGCAAG AAGAATATAT GAAGGAGCAG ATCCCGTGGA CTTTGATTGA CTTCTACGAT 
AACCAACCGT GTATAGACCT CATAGAGGCA AAGCTGGGCA TCCTGGACCT GTTGGATGAG 
GAGTGTAAGG TCCCCAAAGG AACTGATCAG AACTGGGCCC AGAAACTCTA CGAACGACAC 
TCCAACAGCC AGCACTTCCA GAAACCACGC ATGTCCAACA CGGCCTTCAT TGTCAACCAC 
TTTGCAGATA AGGTGGAGTA CCTTTCAGAT GGTTTTCTAG AGAAAAACAG GGATACAGTA 
TATGAAGAAC AGATCAACAT CCTGAAGGCC AGCAAGTTCC CATTAGTGGC TGATTTGTTC 
CACGATGACA AGGACTCTGC TCCAGCCACC AACACAGCTA AGAATCGATC ATCTTCAAAG 
ATCAACGTTC GCTCTTCCAG ACCCCTCATC AAAGTCCCTA ACAAGGAGCA CAAGAAATCC 
GTGGGCTACC AGTTTCGTAC TTCCCTAAAC CTGCTTATGG AGACCCTGAA TGCCACAACG 
CCACACTATG TCCGGTGCAT CAAGCCCAAC GATGAAAAGC TTCCCTTCCA CTTTGACCCC 
AAGAGAGCTG TACAGCAACT CCGAGCCTGC GGTGTGCTGG AGACCATTCG GATCAGCGCG 
GCAGGCTACC CATCCAGGTG GACCTACCAT GACTTCTTCA ACCGGTATCG GGTGTTGATG 
AAGAAGAGAG AGCTCACCAA CACCGACAAG AAGAATATCT GCAAGTCTGT CCTGGAGAGT 
CTCATCAAGG ACCCAGACAA GTTCCAGTTT GGCCGCACCA AGATCTTCTT CCGAGCAGGC 
CAGGTGGCCT ACCTGGAGAA GCTTCGGGCA GACAAGTTCC GGGAAGCTAC TATCATGATC 
CAGAAGTCGG TCAGGGGATG GCTGCAGAGA GTGAAGTACC GTAGGCTGAG GGCAGCTACC 
TTATCCCTGC AGAGGTTCTG CAGGGGATAC CTAGCCCGCA GGCTGGCCGA ACACTTGAGG 
AGAACCCGGG CCGCCATCGT GTTCCAGAAG CAGTACCGCA TGCTGAAGGC CCGCCGAGCC 
TACCGCAGGG TCTGCAGGGC TACCGTCATC ATCCAGTCCT TCACACGGGC CATGTTTGTG 
CGCAGGAACT ACCGCCAGGT CCTCATGGAG CACAAAGCCA CCATCATCCA GAAGTACGCC 
CGAGGCTGGA TGGCACGGAA GCGTTTTCTG CGGGAGCGGG ATGCAGCCAT TGTCATCCAG 
TGTGCCTTCC GGAGACTCAA GGCCAGGCAG GAGCTGAAGG CCCTCAAGAT TGAGGCCCGC 
TCGGCAGAGC ATCTGAAACG CCTCAACGTG GGCATGGAGA ACAAAGTCGT CCAGCTACAG 
CGGAAGATTG ATGACCAGAA CAAAGAGTTC AAGACTCTGT CAGAGCAGCT GTCTGCAGTC 
ACCTCCAGTC ATGCCGTGGA GGTGGAGAAG CTGAAGAAGG AGCTGGCACA TTACCAGCAG 
AACCAGGAGG CTGATACCAG CCTTCAGCTG CAGGAGGAGG TTCAGAGCCT GCGCACTGAA 
CTACAGAAGG CTCATTCGGA GCGCAGGGTC CTGGAGGATG CTCACAACAA GGAGAATGGC 
GAGCTGAGAA AGCGAGTTGC AGACCTGGAA CATGAAAATG CCCTCTTGAA GGATGAAAAA 
GAATACCTTA ACAACCAAAT CCTGTGCCAG TCAAAAGCTG AATCTTCGCA GAGCTCTGTG 
GAAGAGAACC TCCTGATGAA GAAGGAGCTG GAGGAGGAAC GGTCCAGGTA CCAGAACCTC 
GTGAAGGAGT ACTCCCAGCT GGAGCAGAGA TATGAGAACC TTCGGGACGA GGTGACGATC 
CTAAAGCAAA CTCCGGGCCA CAGGAAGAAC CCATCGAACC AAAGCAGCTT AGAATCTGAC 
TCCAATTACC CCTCCATTTC CACTTCCGAA ATCGGAGACA CTGAGGATGC CCTTCAGCAG 
GTGGAGGAGA TTGGCATAGA GAAGGCAGCC ATGGACATGA CCGTCTTCCT GAAGCTGCAG 
AAGAGAGTGC GCGAACTTGA GCAGGAGAGG AAGAAGCTGC AGGCACAGCT AGAAAAGGGA 
CAGCAGGACA GCAAGAAAGG GCAGGTAGAA CAACAGAACA ATGGCTTAGA TGTGGACCAG 
GACGCAGATA TAGCCTACAA TAGTCTGAAG AGACAGGAGC TTGAGTCAGA GAACAAGAAG 
CTGAAGAATG ACCTGAATGA GCTGAGGAAG GCTGTCGCTG ACCAAGCCAT GCAGGATAAC 
TCCACCCACA GCTCCCCAGA CAGCTACAGC CTCCTCCTGA ACCAGCTCAA GCTGGCCAAT 
GAGGAGCTCG AGGTCCGCAA AGAGGAGGTG CTGATCCTCA GGACCCAGAT CATGAATGCC 
GACCAGCGCC GCCTGTCTGG CAAGAACATG GAGCCGAACA TCAATGCCAG AACAAGTTGG 
CCCAACAGTG AGAAGCACGT GGACCAGGAA GACGCCATTG AGGCCTATCA CGGGGTCTGC 
CAGACAAACA GGTTGCTGGA GGCCCAGCTG CAGGCCCAGA GCCTGGAGCA TGAGGAGGAG 
GTGGAACATC TCAAGGCCCA GGTGGAAGCC CTGAAAGAGG AGATGGACAA ACAGCAGCAG 
ACCTTCTGCC AGACCCTGCT GCTCTCCCCA GAGGCCCAGG TAGAATTTGG TGTCCAGCAG 
GAGATATCCC GGCTGACCAA TGAGAACCTG GATTTTAAGG AATTGGTGGA AAAGCTGGAG 
AAGAATGAGA GGAAGCTGAA GAAGCAGCTG AAGATTTACA TGAAGAAGGT CCAGGACTTA 
GAAGCTGCTC AGGCGTTGGC ACAGAGTGAC AGGAGACACC ATGAACTCAC AAGACAGGTC 
ACAGTCCAAC GAAAAGAGAA GGACTTCCAA GGCATGCTGG AGTACCACAA AGAGGACGAA 
GCCCTCCTCA TCCGGAACCT GGTGACAGAC CTGAAGCCTC AGATGCTGTC GGGCACCGTG 
CCCTGTCTGC CTGCATACAT ACTCTATATG TGCATCAGGC ACGCGGATTA CACCAACGAT 
GACCTCAAGG TGCACTCGTT GCTGAGCTCC ACCATCAACG GCATTAAGAA AGTCCTCAAG 
AAGCACAATG ATGACTTTGA GATGACGTCA TTCTGGCTAT CCAACACCTG CCGCTTCCTT 
CACTGTCTGA AGCAATACAG TGGTGATGAG GGTTTCATGA CACAGAACAC TGCGAAGCAG 
AATGAGCACT GTCTCAAGAA CTTTGACCTC ACTGAATACC GCCAGGTGCT AAGCGACCTT 
TCCATTCAGA TCTATCAGCA GCTCATTAAA ATCGCCGAGG GCTTGCTGCA GCCTATGATA 
GTTTCTGCCA TGTTGGAAAA TGAGAGTATC CAGGGGCTGT CTGGTGTGAG ACCAACTGGT 
TACCGGAAGC GCTCCTCCAG CATGGTGGAT GGAGAGAATT CGTATTGCCT GGAGGCCATC 
GTCCGCCAGA TGAATTCTTT CCATACAGTC CTGTGTGACC AGGGTCTGGA CCCCGAGATT 
ATCCTGCAGG TGTTCAAACA GCTCTTCTAC ATGATCAACG CTGTGACTCT TAACAACCTA 
CTCCTGCGGA AAGACGCCTG CTCCTGGAGC ACAGGCATGC AACTCAGGTA CAACATAAGT 
CAACTGGAAG AGTGGCTTCG GGGCAAAAAC CTTCACCAAA GTGGAGCAGT TCAGACCATG 
GAGCCCCTCA TCCAGGCAGC CCAGCTCCTC CAGCTGAAGA AGAAAACCCA CGAGGATGCT 
GAGGCCATCT GCTCTCTGTG CACCTCCCTC AGCACCCAGC AGATTGTCAA AATTTTAAAC 
CTCTACACTC CCTTGAATGA ATTTGAGGAA CGGGTCACAG TGTCCTTCAT ACGAACAATC 
CAGGCTCAGC TACAAGAGAG GAATGACCCT CAGCAGCTCC TGCTGGACTC CAAGCACGTG 
TTCCCAGTTC TGTTTCCATA TAACCCATCT GCTCTGACCA TGGACTCGAT CCACATCCCG 
GCCTGTCTCA ACCTGGAGTT TCTCAATGAA GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5b ( NM_201600.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_963894.1 Mus musculus myosin VB (Myo5b), mRNA.

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CloneID	OMu22045
Clone ID Related Accession (Same CDS sequence)	NM_201600.2
Accession Version	NM_201600.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Vb
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC147992.3, CJ052777.1, CV558709.1, AC148001.6, CF741517.1, AC148002.3, BQ557090.1, CD352410.1, BY245557.1, BU610647.1, DV655888.1 and AK033484.1. On May 12, 2006 this sequence version replaced NM_201600.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC118525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGTACA GCGAGCTCTA CACCCGGTAC ACAAGGGTCT GGATCCCTGA CCCAGATGAG 
GTGTGGCGCT CGGCTGAATT AACCAAGGAC TACAAAGAAG GGGATAAGAG CCTGCAGCTC 
AGACTAGAAG ATGACACAAT TCTAGAATAC CCAGTTGATG TCCAAAATAA CCAGGTGCCA 
TTCTTGCGGA ATCCAGATAT CTTAGTAGGA GAAAACGACC TCACTGCCCT CAGCCACCTC 
CACGAGCCCG CAGTTCTGCA TAACTTAAAG GTCCGTTTCC TGGAATCCAA CCACATCTAC 
ACTTACTGTG GAATTGTGCT TGTTGCCATC AACCCCTACG AGCAGCTGCC AATCTACGGA 
CAGGATGTCA TCTATGCCTA CAGTGGCCAA AACATGGGTG ACATGGACCC CCACATCTTT 
GCTGTGGCAG AAGAAGCCTA CAAGCAGATG GCCAGAGATG AAAAGAACCA ATCCATCATA 
GTCAGCGGAG AGTCTGGAGC AGGCAAGACG GTGTCGGCCA AGTACGCCAT GCGCTATTTT 
GCCACCGTTG GTGGCTCAGC CAGTGATACC AACATTGAAG AGAAGGTCCT AGCATCCAGC 
CCCATCATGG AGGCCATTGG GAACGCCAAG ACTACTCGCA ACGACAACAG CAGCCGATTT 
GGGAAATTCA TTGAGATTGG CTTTGATAAA AAGTACCACA TCATTGGGGC CAACATGAGG 
ACCTACTTGC TGGAGAAGTC CAGGGTGGTC TTTCAGGCGG ATGACGAGAG GAACTACCAC 
ATCTTTTACC AGCTCTGTGC TGCTGCCAGC CTTCCTGAAT TTAAAGAGCT CGCCCTAACG 
TGCGCAGAGG ACTTTTTCTA TACAGCCCAT GGAGGAAACA CGACCATCGA GGGTGTGAAT 
GATGCAGACG ACTTTGAGAA GACTAGACAA GCCCTCACAC TCCTGGGAGT GCGGGATTCC 
CATCAAATAA GCATCTTTAA GATAATTGCT TCTATCTTGC ACCTGGGAAG TGTGGAGATT 
CAGTCTGAGC GGGACGGGGA TTCCTGCAGC ATTTCACCCC AGGATGAACA CTTGAGCAAC 
TTCTGCAGCC TGCTAGGAAT AGAACACAGC CAGATGGAGC ACTGGCTGTG TCATCGGAAG 
CTGGTCACCA CCTCAGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAAGT GGTCAATGCA 
CGTGATGCCT TAGCCAAGCA CATCTATGCT CAGCTGTTCT CCTGGATTGT GGAGCACATC 
AACAAAGCCC TGCACACCTC CCTTAAGCAG CACTCCTTCA TCGGGGTCCT GGATATCTAT 
GGGTTTGAGA CCTTCGAGAT CAATAGCTTT GAACAGTTTT GTATCAACTA CGCCAATGAA 
AAGCTCCAGC AACAGTTCAA TTCGCATGTG TTCAAGCTGG AGCAAGAAGA ATATATGAAG 
GAGCAGATCC CGTGGACTTT GATTGACTTC TACGATAACC AACCGTGTAT AGACCTCATA 
GAGGCAAAGC TGGGCATCCT GGACCTGTTG GATGAGGAGT GTAAGGTCCC CAAAGGAACT 
GATCAGAACT GGGCCCAGAA ACTCTACGAA CGACACTCCA ACAGCCAGCA CTTCCAGAAA 
CCACGCATGT CCAACACGGC CTTCATTGTC AACCACTTTG CAGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTAGAGAA AAACAGGGAT ACAGTATATG AAGAACAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCATT AGTGGCTGAT TTGTTCCACG ATGACAAGGA CTCTGCTCCA 
GCCACCAACA CAGCTAAGAA TCGATCATCT TCAAAGATCA ACGTTCGCTC TTCCAGACCC 
CTCATCAAAG TCCCTAACAA GGAGCACAAG AAATCCGTGG GCTACCAGTT TCGTACTTCC 
CTAAACCTGC TTATGGAGAC CCTGAATGCC ACAACGCCAC ACTATGTCCG GTGCATCAAG 
CCCAACGATG AAAAGCTTCC CTTCCACTTT GACCCCAAGA GAGCTGTACA GCAACTCCGA 
GCCTGCGGTG TGCTGGAGAC CATTCGGATC AGCGCGGCAG GCTACCCATC CAGGTGGACC 
TACCATGACT TCTTCAACCG GTATCGGGTG TTGATGAAGA AGAGAGAGCT CACCAACACC 
GACAAGAAGA ATATCTGCAA GTCTGTCCTG GAGAGTCTCA TCAAGGACCC AGACAAGTTC 
CAGTTTGGCC GCACCAAGAT CTTCTTCCGA GCAGGCCAGG TGGCCTACCT GGAGAAGCTT 
CGGGCAGACA AGTTCCGGGA AGCTACTATC ATGATCCAGA AGTCGGTCAG GGGATGGCTG 
CAGAGAGTGA AGTACCGTAG GCTGAGGGCA GCTACCTTAT CCCTGCAGAG GTTCTGCAGG 
GGATACCTAG CCCGCAGGCT GGCCGAACAC TTGAGGAGAA CCCGGGCCGC CATCGTGTTC 
CAGAAGCAGT ACCGCATGCT GAAGGCCCGC CGAGCCTACC GCAGGGTCTG CAGGGCTACC 
GTCATCATCC AGTCCTTCAC ACGGGCCATG TTTGTGCGCA GGAACTACCG CCAGGTCCTC 
ATGGAGCACA AAGCCACCAT CATCCAGAAG TACGCCCGAG GCTGGATGGC ACGGAAGCGT 
TTTCTGCGGG AGCGGGATGC AGCCATTGTC ATCCAGTGTG CCTTCCGGAG ACTCAAGGCC 
AGGCAGGAGC TGAAGGCCCT CAAGATTGAG GCCCGCTCGG CAGAGCATCT GAAACGCCTC 
AACGTGGGCA TGGAGAACAA AGTCGTCCAG CTACAGCGGA AGATTGATGA CCAGAACAAA 
GAGTTCAAGA CTCTGTCAGA GCAGCTGTCT GCAGTCACCT CCAGTCATGC CGTGGAGGTG 
GAGAAGCTGA AGAAGGAGCT GGCACATTAC CAGCAGAACC AGGAGGCTGA TACCAGCCTT 
CAGCTGCAGG AGGAGGTTCA GAGCCTGCGC ACTGAACTAC AGAAGGCTCA TTCGGAGCGC 
AGGGTCCTGG AGGATGCTCA CAACAAGGAG AATGGCGAGC TGAGAAAGCG AGTTGCAGAC 
CTGGAACATG AAAATGCCCT CTTGAAGGAT GAAAAAGAAT ACCTTAACAA CCAAATCCTG 
TGCCAGTCAA AAGCTGAATC TTCGCAGAGC TCTGTGGAAG AGAACCTCCT GATGAAGAAG 
GAGCTGGAGG AGGAACGGTC CAGGTACCAG AACCTCGTGA AGGAGTACTC CCAGCTGGAG 
CAGAGATATG AGAACCTTCG GGACGAGCAA ACTCCGGGCC ACAGGAAGAA CCCATCGAAC 
CAAAGCAGCT TAGAATCTGA CTCCAATTAC CCCTCCATTT CCACTTCCGA AATCGGAGAC 
ACTGAGGATG CCCTTCAGCA GGTGGAGGAG ATTGGCATAG AGAAGGCAGC CATGGACATG 
ACCGTCTTCC TGAAGCTGCA GAAGAGAGTG CGCGAACTTG AGCAGGAGAG GAAGAAGCTG 
CAGGCACAGC TAGAAAAGGG ACAGCAGGAC AGCAAGAAAG GGCAGGTAGA ACAACAGAAC 
AATGGCTTAG ATGTGGACCA GGACGCAGAT ATAGCCTACA ATAGTCTGAA GAGACAGGAG 
CTTGAGTCAG AGAACAAGAA GCTGAAGAAT GACCTGAATG AGCTGAGGAA GGCTGTCGCT 
GACCAAGCCA TGCAGGATAA CTCCACCCAC AGCTCCCCAG ACAGCTACAG CCTCCTCCTG 
AACCAGCTCA AGCTGGCCAA TGAGGAGCTC GAGGTCCGCA AAGAGGAGGT GCTGATCCTC 
AGGACCCAGA TCATGAATGC CGACCAGCGC CGCCTGTCTG GCAAGAACAT GGAGCCGAAC 
ATCAATGCCA GAACAAGTTG GCCCAACAGT GAGAAGCACG TGGACCAGGA AGACGCCATT 
GAGGCCTATC ACGGGGTCTG CCAGACAAAC AGGTTGCTGG AGGCCCAGCT GCAGGCCCAG 
AGCCTGGAGC ATGAGGAGGA GGTGGAACAT CTCAAGGCCC AGGTGGAAGC CCTGAAAGAG 
GAGATGGACA AACAGCAGCA GACCTTCTGC CAGACCCTGC TGCTCTCCCC AGAGGCCCAG 
GTAGAATTTG GTGTCCAGCA GGAGATATCC CGGCTGACCA ATGAGAACCT GGATTTTAAG 
GAATTGGTGG AAAAGCTGGA GAAGAATGAG AGGAAGCTGA AGAAGCAGCT GAAGATTTAC 
ATGAAGAAGG TCCAGGACTT AGAAGCTGCT CAGGCGTTGG CACAGAGTGA CAGGAGACAC 
CATGAACTCA CAAGACAGGT CACAGTCCAA CGAAAAGAGA AGGACTTCCA AGGCATGCTG 
GAGTACCACA AAGAGGACGA AGCCCTCCTC ATCCGGAACC TGGTGACAGA CCTGAAGCCT 
CAGATGCTGT CGGGCACCGT GCCCTGTCTG CCTGCATACA TACTCTATAT GTGCATCAGG 
CACGCGGATT ACACCAACGA TGACCTCAAG GTGCACTCGT TGCTGAGCTC CACCATCAAC 
GGCATTAAGA AAGTCCTCAA GAAGCACAAT GATGACTTTG AGATGACGTC ATTCTGGCTA 
TCCAACACCT GCCGCTTCCT TCACTGTCTG AAGCAATACA GTGGTGATGA GGGTTTCATG 
ACACAGAACA CTGCGAAGCA GAATGAGCAC TGTCTCAAGA ACTTTGACCT CACTGAATAC 
CGCCAGGTGC TAAGCGACCT TTCCATTCAG ATCTATCAGC AGCTCATTAA AATCGCCGAG 
GGCTTGCTGC AGCCTATGAT AGTTTCTGCC ATGTTGGAAA ATGAGAGTAT CCAGGGGCTG 
TCTGGTGTGA GACCAACTGG TTACCGGAAG CGCTCCTCCA GCATGGTGGA TGGAGAGAAT 
TCGTATTGCC TGGAGGCCAT CGTCCGCCAG ATGAATTCTT TCCATACAGT CCTGTGTGAC 
CAGGGTCTGG ACCCCGAGAT TATCCTGCAG GTGTTCAAAC AGCTCTTCTA CATGATCAAC 
GCTGTGACTC TTAACAACCT ACTCCTGCGG AAAGACGCCT GCTCCTGGAG CACAGGCATG 
CAACTCAGGT ACAACATAAG TCAACTGGAA GAGTGGCTTC GGGGCAAAAA CCTTCACCAA 
AGTGGAGCAG TTCAGACCAT GGAGCCCCTC ATCCAGGCAG CCCAGCTCCT CCAGCTGAAG 
AAGAAAACCC ACGAGGATGC TGAGGCCATC TGCTCTCTGT GCACCTCCCT CAGCACCCAG 
CAGATTGTCA AAATTTTAAA CCTCTACACT CCCTTGAATG AATTTGAGGA ACGGGTCACA 
GTGTCCTTCA TACGAACAAT CCAGGCTCAG CTACAAGAGA GGAATGACCC TCAGCAGCTC 
CTGCTGGACT CCAAGCACGT GTTCCCAGTT CTGTTTCCAT ATAACCCATC TGCTCTGACC 
ATGGACTCGA TCCACATCCC GGCCTGTCTC AACCTGGAGT TTCTCAATGA AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963894.1
CDS	163..5619
Translation	MSYSELYTRYTRVWIPDPDEVWRSAELTKDYKEGDKSLQLRLEDDTILEYPVDVQNNQVPFLRNPDILVGENDLTALSHLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKFIEIGFDKKYHIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTAHGGNTTIEGVNDADDFEKTRQALTLLGVRDSHQISIFKIIASILHLGSVEIQSERDGDSCSISPQDEHLSNFCSLLGIEHSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARDALAKHIYAQLFSWIVEHINKALHTSLKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYERHSNSQHFQKPRMSNTAFIVNHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDDKDSAPATNTAKNRSSSKINVRSSRPLIKVPNKEHKKSVGYQFRTSLNLLMETLNATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWTYHDFFNRYRVLMKKRELTNTDKKNICKSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFREATIMIQKSVRGWLQRVKYRRLRAATLSLQRFCRGYLARRLAEHLRRTRAAIVFQKQYRMLKARRAYRRVCRATVIIQSFTRAMFVRRNYRQVLMEHKATIIQKYARGWMARKRFLRERDAAIVIQCAFRRLKARQELKALKIEARSAEHLKRLNVGMENKVVQLQRKIDDQNKEFKTLSEQLSAVTSSHAVEVEKLKKELAHYQQNQEADTSLQLQEEVQSLRTELQKAHSERRVLEDAHNKENGELRKRVADLEHENALLKDEKEYLNNQILCQSKAESSQSSVEENLLMKKELEEERSRYQNLVKEYSQLEQRYENLRDEQTPGHRKNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGIEKAAMDMTVFLKLQKRVRELEQERKKLQAQLEKGQQDSKKGQVEQQNNGLDVDQDADIAYNSLKRQELESENKKLKNDLNELRKAVADQAMQDNSTHSSPDSYSLLLNQLKLANEELEVRKEEVLILRTQIMNADQRRLSGKNMEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNRLLEAQLQAQSLEHEEEVEHLKAQVEALKEEMDKQQQTFCQTLLLSPEAQVEFGVQQEISRLTNENLDFKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSDRRHHELTRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTDLKPQMLSGTVPCLPAYILYMCIRHADYTNDDLKVHSLLSSTINGIKKVLKKHNDDFEMTSFWLSNTCRFLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGLLQPMIVSAMLENESIQGLSGVRPTGYRKRSSSMVDGENSYCLEAIVRQMNSFHTVLCDQGLDPEIILQVFKQLFYMINAVTLNNLLLRKDACSWSTGMQLRYNISQLEEWLRGKNLHQSGAVQTMEPLIQAAQLLQLKKKTHEDAEAICSLCTSLSTQQIVKILNLYTPLNEFEERVTVSFIRTIQAQLQERNDPQQLLLDSKHVFPVLFPYNPSALTMDSIHIPACLNLEFLNEV

Target ORF information:

RefSeq Version	NM_201600.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VB (Myo5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201600.2

ORF Insert Sequence:

ATGTCGTACA GCGAGCTCTA CACCCGGTAC ACAAGGGTCT GGATCCCTGA CCCAGATGAG 
GTGTGGCGCT CGGCTGAATT AACCAAGGAC TACAAAGAAG GGGATAAGAG CCTGCAGCTC 
AGACTAGAAG ATGACACAAT TCTAGAATAC CCAGTTGATG TCCAAAATAA CCAGGTGCCA 
TTCTTGCGGA ATCCAGATAT CTTAGTAGGA GAAAACGACC TCACTGCCCT CAGCCACCTC 
CACGAGCCCG CAGTTCTGCA TAACTTAAAG GTCCGTTTCC TGGAATCCAA CCACATCTAC 
ACTTACTGTG GAATTGTGCT TGTTGCCATC AACCCCTACG AGCAGCTGCC AATCTACGGA 
CAGGATGTCA TCTATGCCTA CAGTGGCCAA AACATGGGTG ACATGGACCC CCACATCTTT 
GCTGTGGCAG AAGAAGCCTA CAAGCAGATG GCCAGAGATG AAAAGAACCA ATCCATCATA 
GTCAGCGGAG AGTCTGGAGC AGGCAAGACG GTGTCGGCCA AGTACGCCAT GCGCTATTTT 
GCCACCGTTG GTGGCTCAGC CAGTGATACC AACATTGAAG AGAAGGTCCT AGCATCCAGC 
CCCATCATGG AGGCCATTGG GAACGCCAAG ACTACTCGCA ACGACAACAG CAGCCGATTT 
GGGAAATTCA TTGAGATTGG CTTTGATAAA AAGTACCACA TCATTGGGGC CAACATGAGG 
ACCTACTTGC TGGAGAAGTC CAGGGTGGTC TTTCAGGCGG ATGACGAGAG GAACTACCAC 
ATCTTTTACC AGCTCTGTGC TGCTGCCAGC CTTCCTGAAT TTAAAGAGCT CGCCCTAACG 
TGCGCAGAGG ACTTTTTCTA TACAGCCCAT GGAGGAAACA CGACCATCGA GGGTGTGAAT 
GATGCAGACG ACTTTGAGAA GACTAGACAA GCCCTCACAC TCCTGGGAGT GCGGGATTCC 
CATCAAATAA GCATCTTTAA GATAATTGCT TCTATCTTGC ACCTGGGAAG TGTGGAGATT 
CAGTCTGAGC GGGACGGGGA TTCCTGCAGC ATTTCACCCC AGGATGAACA CTTGAGCAAC 
TTCTGCAGCC TGCTAGGAAT AGAACACAGC CAGATGGAGC ACTGGCTGTG TCATCGGAAG 
CTGGTCACCA CCTCAGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAAGT GGTCAATGCA 
CGTGATGCCT TAGCCAAGCA CATCTATGCT CAGCTGTTCT CCTGGATTGT GGAGCACATC 
AACAAAGCCC TGCACACCTC CCTTAAGCAG CACTCCTTCA TCGGGGTCCT GGATATCTAT 
GGGTTTGAGA CCTTCGAGAT CAATAGCTTT GAACAGTTTT GTATCAACTA CGCCAATGAA 
AAGCTCCAGC AACAGTTCAA TTCGCATGTG TTCAAGCTGG AGCAAGAAGA ATATATGAAG 
GAGCAGATCC CGTGGACTTT GATTGACTTC TACGATAACC AACCGTGTAT AGACCTCATA 
GAGGCAAAGC TGGGCATCCT GGACCTGTTG GATGAGGAGT GTAAGGTCCC CAAAGGAACT 
GATCAGAACT GGGCCCAGAA ACTCTACGAA CGACACTCCA ACAGCCAGCA CTTCCAGAAA 
CCACGCATGT CCAACACGGC CTTCATTGTC AACCACTTTG CAGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTAGAGAA AAACAGGGAT ACAGTATATG AAGAACAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCATT AGTGGCTGAT TTGTTCCACG ATGACAAGGA CTCTGCTCCA 
GCCACCAACA CAGCTAAGAA TCGATCATCT TCAAAGATCA ACGTTCGCTC TTCCAGACCC 
CTCATCAAAG TCCCTAACAA GGAGCACAAG AAATCCGTGG GCTACCAGTT TCGTACTTCC 
CTAAACCTGC TTATGGAGAC CCTGAATGCC ACAACGCCAC ACTATGTCCG GTGCATCAAG 
CCCAACGATG AAAAGCTTCC CTTCCACTTT GACCCCAAGA GAGCTGTACA GCAACTCCGA 
GCCTGCGGTG TGCTGGAGAC CATTCGGATC AGCGCGGCAG GCTACCCATC CAGGTGGACC 
TACCATGACT TCTTCAACCG GTATCGGGTG TTGATGAAGA AGAGAGAGCT CACCAACACC 
GACAAGAAGA ATATCTGCAA GTCTGTCCTG GAGAGTCTCA TCAAGGACCC AGACAAGTTC 
CAGTTTGGCC GCACCAAGAT CTTCTTCCGA GCAGGCCAGG TGGCCTACCT GGAGAAGCTT 
CGGGCAGACA AGTTCCGGGA AGCTACTATC ATGATCCAGA AGTCGGTCAG GGGATGGCTG 
CAGAGAGTGA AGTACCGTAG GCTGAGGGCA GCTACCTTAT CCCTGCAGAG GTTCTGCAGG 
GGATACCTAG CCCGCAGGCT GGCCGAACAC TTGAGGAGAA CCCGGGCCGC CATCGTGTTC 
CAGAAGCAGT ACCGCATGCT GAAGGCCCGC CGAGCCTACC GCAGGGTCTG CAGGGCTACC 
GTCATCATCC AGTCCTTCAC ACGGGCCATG TTTGTGCGCA GGAACTACCG CCAGGTCCTC 
ATGGAGCACA AAGCCACCAT CATCCAGAAG TACGCCCGAG GCTGGATGGC ACGGAAGCGT 
TTTCTGCGGG AGCGGGATGC AGCCATTGTC ATCCAGTGTG CCTTCCGGAG ACTCAAGGCC 
AGGCAGGAGC TGAAGGCCCT CAAGATTGAG GCCCGCTCGG CAGAGCATCT GAAACGCCTC 
AACGTGGGCA TGGAGAACAA AGTCGTCCAG CTACAGCGGA AGATTGATGA CCAGAACAAA 
GAGTTCAAGA CTCTGTCAGA GCAGCTGTCT GCAGTCACCT CCAGTCATGC CGTGGAGGTG 
GAGAAGCTGA AGAAGGAGCT GGCACATTAC CAGCAGAACC AGGAGGCTGA TACCAGCCTT 
CAGCTGCAGG AGGAGGTTCA GAGCCTGCGC ACTGAACTAC AGAAGGCTCA TTCGGAGCGC 
AGGGTCCTGG AGGATGCTCA CAACAAGGAG AATGGCGAGC TGAGAAAGCG AGTTGCAGAC 
CTGGAACATG AAAATGCCCT CTTGAAGGAT GAAAAAGAAT ACCTTAACAA CCAAATCCTG 
TGCCAGTCAA AAGCTGAATC TTCGCAGAGC TCTGTGGAAG AGAACCTCCT GATGAAGAAG 
GAGCTGGAGG AGGAACGGTC CAGGTACCAG AACCTCGTGA AGGAGTACTC CCAGCTGGAG 
CAGAGATATG AGAACCTTCG GGACGAGCAA ACTCCGGGCC ACAGGAAGAA CCCATCGAAC 
CAAAGCAGCT TAGAATCTGA CTCCAATTAC CCCTCCATTT CCACTTCCGA AATCGGAGAC 
ACTGAGGATG CCCTTCAGCA GGTGGAGGAG ATTGGCATAG AGAAGGCAGC CATGGACATG 
ACCGTCTTCC TGAAGCTGCA GAAGAGAGTG CGCGAACTTG AGCAGGAGAG GAAGAAGCTG 
CAGGCACAGC TAGAAAAGGG ACAGCAGGAC AGCAAGAAAG GGCAGGTAGA ACAACAGAAC 
AATGGCTTAG ATGTGGACCA GGACGCAGAT ATAGCCTACA ATAGTCTGAA GAGACAGGAG 
CTTGAGTCAG AGAACAAGAA GCTGAAGAAT GACCTGAATG AGCTGAGGAA GGCTGTCGCT 
GACCAAGCCA TGCAGGATAA CTCCACCCAC AGCTCCCCAG ACAGCTACAG CCTCCTCCTG 
AACCAGCTCA AGCTGGCCAA TGAGGAGCTC GAGGTCCGCA AAGAGGAGGT GCTGATCCTC 
AGGACCCAGA TCATGAATGC CGACCAGCGC CGCCTGTCTG GCAAGAACAT GGAGCCGAAC 
ATCAATGCCA GAACAAGTTG GCCCAACAGT GAGAAGCACG TGGACCAGGA AGACGCCATT 
GAGGCCTATC ACGGGGTCTG CCAGACAAAC AGGTTGCTGG AGGCCCAGCT GCAGGCCCAG 
AGCCTGGAGC ATGAGGAGGA GGTGGAACAT CTCAAGGCCC AGGTGGAAGC CCTGAAAGAG 
GAGATGGACA AACAGCAGCA GACCTTCTGC CAGACCCTGC TGCTCTCCCC AGAGGCCCAG 
GTAGAATTTG GTGTCCAGCA GGAGATATCC CGGCTGACCA ATGAGAACCT GGATTTTAAG 
GAATTGGTGG AAAAGCTGGA GAAGAATGAG AGGAAGCTGA AGAAGCAGCT GAAGATTTAC 
ATGAAGAAGG TCCAGGACTT AGAAGCTGCT CAGGCGTTGG CACAGAGTGA CAGGAGACAC 
CATGAACTCA CAAGACAGGT CACAGTCCAA CGAAAAGAGA AGGACTTCCA AGGCATGCTG 
GAGTACCACA AAGAGGACGA AGCCCTCCTC ATCCGGAACC TGGTGACAGA CCTGAAGCCT 
CAGATGCTGT CGGGCACCGT GCCCTGTCTG CCTGCATACA TACTCTATAT GTGCATCAGG 
CACGCGGATT ACACCAACGA TGACCTCAAG GTGCACTCGT TGCTGAGCTC CACCATCAAC 
GGCATTAAGA AAGTCCTCAA GAAGCACAAT GATGACTTTG AGATGACGTC ATTCTGGCTA 
TCCAACACCT GCCGCTTCCT TCACTGTCTG AAGCAATACA GTGGTGATGA GGGTTTCATG 
ACACAGAACA CTGCGAAGCA GAATGAGCAC TGTCTCAAGA ACTTTGACCT CACTGAATAC 
CGCCAGGTGC TAAGCGACCT TTCCATTCAG ATCTATCAGC AGCTCATTAA AATCGCCGAG 
GGCTTGCTGC AGCCTATGAT AGTTTCTGCC ATGTTGGAAA ATGAGAGTAT CCAGGGGCTG 
TCTGGTGTGA GACCAACTGG TTACCGGAAG CGCTCCTCCA GCATGGTGGA TGGAGAGAAT 
TCGTATTGCC TGGAGGCCAT CGTCCGCCAG ATGAATTCTT TCCATACAGT CCTGTGTGAC 
CAGGGTCTGG ACCCCGAGAT TATCCTGCAG GTGTTCAAAC AGCTCTTCTA CATGATCAAC 
GCTGTGACTC TTAACAACCT ACTCCTGCGG AAAGACGCCT GCTCCTGGAG CACAGGCATG 
CAACTCAGGT ACAACATAAG TCAACTGGAA GAGTGGCTTC GGGGCAAAAA CCTTCACCAA 
AGTGGAGCAG TTCAGACCAT GGAGCCCCTC ATCCAGGCAG CCCAGCTCCT CCAGCTGAAG 
AAGAAAACCC ACGAGGATGC TGAGGCCATC TGCTCTCTGT GCACCTCCCT CAGCACCCAG 
CAGATTGTCA AAATTTTAAA CCTCTACACT CCCTTGAATG AATTTGAGGA ACGGGTCACA 
GTGTCCTTCA TACGAACAAT CCAGGCTCAG CTACAAGAGA GGAATGACCC TCAGCAGCTC 
CTGCTGGACT CCAAGCACGT GTTCCCAGTT CTGTTTCCAT ATAACCCATC TGCTCTGACC 
ATGGACTCGA TCCACATCCC GGCCTGTCTC AACCTGGAGT TTCTCAATGA AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5b ( XM_006525714.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525777.1 Mus musculus myosin VB (Myo5b), transcript variant X1, mRNA.

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CloneID	OMu54430
Clone ID Related Accession (Same CDS sequence)	XM_006525714.4 , XM_006525714.3
Accession Version	XM_006525714.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Vb isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525714.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTACA GCGAGCTCTA CACCCGGTAC ACAAGGGTCT GGATCCCTGA CCCAGATGAG 
GTGTGGCGCT CGGCTGAATT AACCAAGGAC TACAAAGAAG GGGATAAGAG CCTGCAGCTC 
AGACTAGAAG ATGACACAAT TCTAGAATAC CCAGTTGATG TCCAAAATAA CCAGGTGCCA 
TTCTTGCGGA ATCCAGATAT CTTAGTAGGA GAAAACGACC TCACTGCCCT CAGCCACCTC 
CACGAGCCCG CAGTTCTGCA TAACTTAAAG GTCCGTTTCC TGGAATCCAA CCACATCTAC 
ACTTACTGTG GAATTGTGCT TGTTGCCATC AACCCCTACG AGCAGCTGCC AATCTACGGA 
CAGGATGTCA TCTATGCCTA CAGTGGCCAA AACATGGGTG ACATGGACCC CCACATCTTT 
GCTGTGGCAG AAGAAGCCTA CAAGCAGATG GCCAGAGATG AAAAGAACCA ATCCATCATA 
GTCAGCGGAG AGTCTGGAGC AGGCAAGACG GTGTCGGCCA AGTACGCCAT GCGCTATTTT 
GCCACCGTTG GTGGCTCAGC CAGTGATACC AACATTGAAG AGAAGGTCCT AGCATCCAGC 
CCCATCATGG AGGCCATTGG GAACGCCAAG ACTACTCGCA ACGACAACAG CAGCCGATTT 
GGGAAATTCA TTGAGATTGG CTTTGATAAA AAGTACCACA TCATTGGGGC CAACATGAGG 
ACCTACTTGC TGGAGAAGTC CAGGGTGGTC TTTCAGGCGG ATGACGAGAG GAACTACCAC 
ATCTTTTACC AGCTCTGTGC TGCTGCCAGC CTTCCTGAAT TTAAAGAGCT CGCCCTAACG 
TGCGCAGAGG ACTTTTTCTA TACAGCCCAT GGAGGAAACA CGACCATCGA GGGTGTGAAT 
GATGCAGACG ACTTTGAGAA GACTAGACAA GCCCTCACAC TCCTGGGAGT GCGGGATTCC 
CATCAAATAA GCATCTTTAA GATAATTGCT TCTATCTTGC ACCTGGGAAG TGTGGAGATT 
CAGTCTGAGC GGGACGGGGA TTCCTGCAGC ATTTCACCCC AGGATGAACA CTTGAGCAAC 
TTCTGCAGCC TGCTAGGAAT AGAACACAGC CAGATGGAGC ACTGGCTGTG TCATCGGAAG 
CTGGTCACCA CCTCAGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAAGT GGTCAATGCA 
CGTGATGCCT TAGCCAAGCA CATCTATGCT CAGCTGTTCT CCTGGATTGT GGAGCACATC 
AACAAAGCCC TGCACACCTC CCTTAAGCAG CACTCCTTCA TCGGGGTCCT GGATATCTAT 
GGGTTTGAGA CCTTCGAGAT CAATAGCTTT GAACAGTTTT GTATCAACTA CGCCAATGAA 
AAGCTCCAGC AACAGTTCAA TTCGCATGTG TTCAAGCTGG AGCAAGAAGA ATATATGAAG 
GAGCAGATCC CGTGGACTTT GATTGACTTC TACGATAACC AACCGTGTAT AGACCTCATA 
GAGGCAAAGC TGGGCATCCT GGACCTGTTG GATGAGGAGT GTAAGGTCCC CAAAGGAACT 
GATCAGAACT GGGCCCAGAA ACTCTACGAA CGACACTCCA ACAGCCAGCA CTTCCAGAAA 
CCACGCATGT CCAACACGGC CTTCATTGTC AACCACTTTG CAGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTAGAGAA AAACAGGGAT ACAGTATATG AAGAACAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCATT AGTGGCTGAT TTGTTCCACG ATGACAAGGA CTCTGCTCCA 
GCCACCAACA CAGCTAAGAA TCGATCATCT TCAAAGATCA ACGTTCGCTC TTCCAGACCC 
CTCATCAAAG TCCCTAACAA GGAGCACAAG AAATCCGTGG GCTACCAGTT TCGTACTTCC 
CTAAACCTGC TTATGGAGAC CCTGAATGCC ACAACGCCAC ACTATGTCCG GTGCATCAAG 
CCCAACGATG AAAAGCTTCC CTTCCACTTT GACCCCAAGA GAGCTGTACA GCAACTCCGA 
GCCTGCGGTG TGCTGGAGAC CATTCGGATC AGCGCGGCAG GCTACCCATC CAGGTGGACC 
TACCATGACT TCTTCAACCG GTATCGGGTG TTGATGAAGA AGAGAGAGCT CACCAACACC 
GACAAGAAGA ATATCTGCAA GTCTGTCCTG GAGAGTCTCA TCAAGGACCC AGACAAGTTC 
CAGTTTGGCC GCACCAAGAT CTTCTTCCGA GCAGGCCAGG TGGCCTACCT GGAGAAGCTT 
CGGGCAGACA AGTTCCGGGA AGCTACTATC ATGATCCAGA AGTCGGTCAG GGGATGGCTG 
CAGAGAGTGA AGTACCGTAG GCTGAGGGCA GCTACCTTAT CCCTGCAGAG GTTCTGCAGG 
GGATACCTAG CCCGCAGGCT GGCCGAACAC TTGAGGAGAA CCCGGGCCGC CATCGTGTTC 
CAGAAGCAGT ACCGCATGCT GAAGGCCCGC CGAGCCTACC GCAGGGTCTG CAGGGCTACC 
GTCATCATCC AGTCCTTCAC ACGGGCCATG TTTGTGCGCA GGAACTACCG CCAGGTCCTC 
ATGGAGCACA AAGCCACCAT CATCCAGAAG TACGCCCGAG GCTGGATGGC ACGGAAGCGT 
TTTCTGCGGG AGCGGGATGC AGCCATTGTC ATCCAGTGTG CCTTCCGGAG ACTCAAGGCC 
AGGCAGGAGC TGAAGGCCCT CAAGATTGAG GCCCGCTCGG CAGAGCATCT GAAACGCCTC 
AACGTGGGCA TGGAGAACAA AGTCGTCCAG CTACAGCGGA AGATTGATGA CCAGAACAAA 
GAGTTCAAGA CTCTGTCAGA GCAGCTGTCT GCAGTCACCT CCAGTCATGC CGTGGAGGTG 
GAGAAGCTGA AGAAGGAGCT GGCACATTAC CAGCAGAACC AGGAGGCTGA TACCAGCCTT 
CAGCTGCAGG AGGAGGTTCA GAGCCTGCGC ACTGAACTAC AGAAGGCTCA TTCGGAGCGC 
AGGGTCCTGG AGGATGCTCA CAACAAGGAG AATGGCGAGC TGAGAAAGCG AGTTGCAGAC 
CTGGAACATG AAAATGCCCT CTTGAAGGAT GAAAAAGAAT ACCTTAACAA CCAAATCCTG 
TGCCAGTCAA AAGCTGAATC TTCGCAGAGC TCTGTGGAAG AGAACCTCCT GATGAAGAAG 
GAGCTGGAGG AGGAACGGTC CAGGTACCAG AACCTCGTGA AGGAGTACTC CCAGCTGGAG 
CAGAGATATG AGAACCTTCG GGACGAGGTG ACGATCCTAA AGCAAACTCC GGGCCACAGG 
AAGAACCCAT CGAACCAAAG CAGCTTAGAA TCTGACTCCA ATTACCCCTC CATTTCCACT 
TCCGAAATCG GAGACACTGA GGATGCCCTT CAGCAGGTGG AGGAGATTGG CATAGAGAAG 
GCAGCCATGG ACATGACCGT CTTCCTGAAG CTGCAGAAGA GAGTGCGCGA ACTTGAGCAG 
GAGAGGAAGA AGCTGCAGGC ACAGCTAGAA AAGGGACAGC AGGACAGCAA GAAAGGGCAG 
GTAGAACAAC AGAACAATGG CTTAGATGTG GACCAGGACG CAGATATAGC CTACAATAGT 
CTGAAGAGAC AGGAGCTTGA GTCAGAGAAC AAGAAGCTGA AGAATGACCT GAATGAGCTG 
AGGAAGGCTG TCGCTGACCA AGCCATGCAG GATAACTCCA CCCACAGCTC CCCAGACAGC 
TACAGCCTCC TCCTGAACCA GCTCAAGCTG GCCAATGAGG AGCTCGAGGT CCGCAAAGAG 
GAGGTGCTGA TCCTCAGGAC CCAGATCATG AATGCCGACC AGCGCCGCCT GTCTGGCAAG 
AACATGGAGC CGAACATCAA TGCCAGAACA AGTTGGCCCA ACAGTGAGAA GCACGTGGAC 
CAGGAAGACG CCATTGAGGC CTATCACGGG GTCTGCCAGA CAAACAGCCA GACTGAGGAT 
TGGGGATATT TGAATGAAGA TGGAGAACTC GGCTTGGCTT ACCAAGGCCT AAAGCAAGTT 
GCCAGGTTGC TGGAGGCCCA GCTGCAGGCC CAGAGCCTGG AGCATGAGGA GGAGGTGGAA 
CATCTCAAGG CCCAGGTGGA AGCCCTGAAA GAGGAGATGG ACAAACAGCA GCAGACCTTC 
TGCCAGACCC TGCTGCTCTC CCCAGAGGCC CAGGTAGAAT TTGGTGTCCA GCAGGAGATA 
TCCCGGCTGA CCAATGAGAA CCTGGATTTT AAGGAATTGG TGGAAAAGCT GGAGAAGAAT 
GAGAGGAAGC TGAAGAAGCA GCTGAAGATT TACATGAAGA AGGTCCAGGA CTTAGAAGCT 
GCTCAGGCGT TGGCACAGAG TGACAGGAGA CACCATGAAC TCACAAGACA GGTCACAGTC 
CAACGAAAAG AGAAGGACTT CCAAGGCATG CTGGAGTACC ACAAAGAGGA CGAAGCCCTC 
CTCATCCGGA ACCTGGTGAC AGACCTGAAG CCTCAGATGC TGTCGGGCAC CGTGCCCTGT 
CTGCCTGCAT ACATACTCTA TATGTGCATC AGGCACGCGG ATTACACCAA CGATGACCTC 
AAGGTGCACT CGTTGCTGAG CTCCACCATC AACGGCATTA AGAAAGTCCT CAAGAAGCAC 
AATGATGACT TTGAGATGAC GTCATTCTGG CTATCCAACA CCTGCCGCTT CCTTCACTGT 
CTGAAGCAAT ACAGTGGTGA TGAGGGTTTC ATGACACAGA ACACTGCGAA GCAGAATGAG 
CACTGTCTCA AGAACTTTGA CCTCACTGAA TACCGCCAGG TGCTAAGCGA CCTTTCCATT 
CAGATCTATC AGCAGCTCAT TAAAATCGCC GAGGGCTTGC TGCAGCCTAT GATAGTTTCT 
GCCATGTTGG AAAATGAGAG TATCCAGGGG CTGTCTGGTG TGAGACCAAC TGGTTACCGG 
AAGCGCTCCT CCAGCATGGT GGATGGAGAG AATTCGTATT GCCTGGAGGC CATCGTCCGC 
CAGATGAATT CTTTCCATAC AGTCCTGTGT GACCAGGGTC TGGACCCCGA GATTATCCTG 
CAGGTGTTCA AACAGCTCTT CTACATGATC AACGCTGTGA CTCTTAACAA CCTACTCCTG 
CGGAAAGACG CCTGCTCCTG GAGCACAGGC ATGCAACTCA GGTACAACAT AAGTCAACTG 
GAAGAGTGGC TTCGGGGCAA AAACCTTCAC CAAAGTGGAG CAGTTCAGAC CATGGAGCCC 
CTCATCCAGG CAGCCCAGCT CCTCCAGCTG AAGAAGAAAA CCCACGAGGA TGCTGAGGCC 
ATCTGCTCTC TGTGCACCTC CCTCAGCACC CAGCAGATTG TCAAAATTTT AAACCTCTAC 
ACTCCCTTGA ATGAATTTGA GGAACGGGTC ACAGTGTCCT TCATACGAAC AATCCAGGCT 
CAGCTACAAG AGAGGAATGA CCCTCAGCAG CTCCTGCTGG ACTCCAAGCA CGTGTTCCCA 
GTTCTGTTTC CATATAACCC ATCTGCTCTG ACCATGGACT CGATCCACAT CCCGGCCTGT 
CTCAACCTGG AGTTTCTCAA TGAAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525777.1
CDS	402..5951
Translation	MSYSELYTRYTRVWIPDPDEVWRSAELTKDYKEGDKSLQLRLEDDTILEYPVDVQNNQVPFLRNPDILVGENDLTALSHLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKFIEIGFDKKYHIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTAHGGNTTIEGVNDADDFEKTRQALTLLGVRDSHQISIFKIIASILHLGSVEIQSERDGDSCSISPQDEHLSNFCSLLGIEHSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARDALAKHIYAQLFSWIVEHINKALHTSLKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYERHSNSQHFQKPRMSNTAFIVNHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDDKDSAPATNTAKNRSSSKINVRSSRPLIKVPNKEHKKSVGYQFRTSLNLLMETLNATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWTYHDFFNRYRVLMKKRELTNTDKKNICKSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFREATIMIQKSVRGWLQRVKYRRLRAATLSLQRFCRGYLARRLAEHLRRTRAAIVFQKQYRMLKARRAYRRVCRATVIIQSFTRAMFVRRNYRQVLMEHKATIIQKYARGWMARKRFLRERDAAIVIQCAFRRLKARQELKALKIEARSAEHLKRLNVGMENKVVQLQRKIDDQNKEFKTLSEQLSAVTSSHAVEVEKLKKELAHYQQNQEADTSLQLQEEVQSLRTELQKAHSERRVLEDAHNKENGELRKRVADLEHENALLKDEKEYLNNQILCQSKAESSQSSVEENLLMKKELEEERSRYQNLVKEYSQLEQRYENLRDEVTILKQTPGHRKNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGIEKAAMDMTVFLKLQKRVRELEQERKKLQAQLEKGQQDSKKGQVEQQNNGLDVDQDADIAYNSLKRQELESENKKLKNDLNELRKAVADQAMQDNSTHSSPDSYSLLLNQLKLANEELEVRKEEVLILRTQIMNADQRRLSGKNMEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNSQTEDWGYLNEDGELGLAYQGLKQVARLLEAQLQAQSLEHEEEVEHLKAQVEALKEEMDKQQQTFCQTLLLSPEAQVEFGVQQEISRLTNENLDFKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSDRRHHELTRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTDLKPQMLSGTVPCLPAYILYMCIRHADYTNDDLKVHSLLSSTINGIKKVLKKHNDDFEMTSFWLSNTCRFLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGLLQPMIVSAMLENESIQGLSGVRPTGYRKRSSSMVDGENSYCLEAIVRQMNSFHTVLCDQGLDPEIILQVFKQLFYMINAVTLNNLLLRKDACSWSTGMQLRYNISQLEEWLRGKNLHQSGAVQTMEPLIQAAQLLQLKKKTHEDAEAICSLCTSLSTQQIVKILNLYTPLNEFEERVTVSFIRTIQAQLQERNDPQQLLLDSKHVFPVLFPYNPSALTMDSIHIPACLNLEFLNEV

Target ORF information:

RefSeq Version	XM_006525714.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VB (Myo5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525714.3

ORF Insert Sequence:

ATGTCGTACA GCGAGCTCTA CACCCGGTAC ACAAGGGTCT GGATCCCTGA CCCAGATGAG 
GTGTGGCGCT CGGCTGAATT AACCAAGGAC TACAAAGAAG GGGATAAGAG CCTGCAGCTC 
AGACTAGAAG ATGACACAAT TCTAGAATAC CCAGTTGATG TCCAAAATAA CCAGGTGCCA 
TTCTTGCGGA ATCCAGATAT CTTAGTAGGA GAAAACGACC TCACTGCCCT CAGCCACCTC 
CACGAGCCCG CAGTTCTGCA TAACTTAAAG GTCCGTTTCC TGGAATCCAA CCACATCTAC 
ACTTACTGTG GAATTGTGCT TGTTGCCATC AACCCCTACG AGCAGCTGCC AATCTACGGA 
CAGGATGTCA TCTATGCCTA CAGTGGCCAA AACATGGGTG ACATGGACCC CCACATCTTT 
GCTGTGGCAG AAGAAGCCTA CAAGCAGATG GCCAGAGATG AAAAGAACCA ATCCATCATA 
GTCAGCGGAG AGTCTGGAGC AGGCAAGACG GTGTCGGCCA AGTACGCCAT GCGCTATTTT 
GCCACCGTTG GTGGCTCAGC CAGTGATACC AACATTGAAG AGAAGGTCCT AGCATCCAGC 
CCCATCATGG AGGCCATTGG GAACGCCAAG ACTACTCGCA ACGACAACAG CAGCCGATTT 
GGGAAATTCA TTGAGATTGG CTTTGATAAA AAGTACCACA TCATTGGGGC CAACATGAGG 
ACCTACTTGC TGGAGAAGTC CAGGGTGGTC TTTCAGGCGG ATGACGAGAG GAACTACCAC 
ATCTTTTACC AGCTCTGTGC TGCTGCCAGC CTTCCTGAAT TTAAAGAGCT CGCCCTAACG 
TGCGCAGAGG ACTTTTTCTA TACAGCCCAT GGAGGAAACA CGACCATCGA GGGTGTGAAT 
GATGCAGACG ACTTTGAGAA GACTAGACAA GCCCTCACAC TCCTGGGAGT GCGGGATTCC 
CATCAAATAA GCATCTTTAA GATAATTGCT TCTATCTTGC ACCTGGGAAG TGTGGAGATT 
CAGTCTGAGC GGGACGGGGA TTCCTGCAGC ATTTCACCCC AGGATGAACA CTTGAGCAAC 
TTCTGCAGCC TGCTAGGAAT AGAACACAGC CAGATGGAGC ACTGGCTGTG TCATCGGAAG 
CTGGTCACCA CCTCAGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAAGT GGTCAATGCA 
CGTGATGCCT TAGCCAAGCA CATCTATGCT CAGCTGTTCT CCTGGATTGT GGAGCACATC 
AACAAAGCCC TGCACACCTC CCTTAAGCAG CACTCCTTCA TCGGGGTCCT GGATATCTAT 
GGGTTTGAGA CCTTCGAGAT CAATAGCTTT GAACAGTTTT GTATCAACTA CGCCAATGAA 
AAGCTCCAGC AACAGTTCAA TTCGCATGTG TTCAAGCTGG AGCAAGAAGA ATATATGAAG 
GAGCAGATCC CGTGGACTTT GATTGACTTC TACGATAACC AACCGTGTAT AGACCTCATA 
GAGGCAAAGC TGGGCATCCT GGACCTGTTG GATGAGGAGT GTAAGGTCCC CAAAGGAACT 
GATCAGAACT GGGCCCAGAA ACTCTACGAA CGACACTCCA ACAGCCAGCA CTTCCAGAAA 
CCACGCATGT CCAACACGGC CTTCATTGTC AACCACTTTG CAGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTAGAGAA AAACAGGGAT ACAGTATATG AAGAACAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCATT AGTGGCTGAT TTGTTCCACG ATGACAAGGA CTCTGCTCCA 
GCCACCAACA CAGCTAAGAA TCGATCATCT TCAAAGATCA ACGTTCGCTC TTCCAGACCC 
CTCATCAAAG TCCCTAACAA GGAGCACAAG AAATCCGTGG GCTACCAGTT TCGTACTTCC 
CTAAACCTGC TTATGGAGAC CCTGAATGCC ACAACGCCAC ACTATGTCCG GTGCATCAAG 
CCCAACGATG AAAAGCTTCC CTTCCACTTT GACCCCAAGA GAGCTGTACA GCAACTCCGA 
GCCTGCGGTG TGCTGGAGAC CATTCGGATC AGCGCGGCAG GCTACCCATC CAGGTGGACC 
TACCATGACT TCTTCAACCG GTATCGGGTG TTGATGAAGA AGAGAGAGCT CACCAACACC 
GACAAGAAGA ATATCTGCAA GTCTGTCCTG GAGAGTCTCA TCAAGGACCC AGACAAGTTC 
CAGTTTGGCC GCACCAAGAT CTTCTTCCGA GCAGGCCAGG TGGCCTACCT GGAGAAGCTT 
CGGGCAGACA AGTTCCGGGA AGCTACTATC ATGATCCAGA AGTCGGTCAG GGGATGGCTG 
CAGAGAGTGA AGTACCGTAG GCTGAGGGCA GCTACCTTAT CCCTGCAGAG GTTCTGCAGG 
GGATACCTAG CCCGCAGGCT GGCCGAACAC TTGAGGAGAA CCCGGGCCGC CATCGTGTTC 
CAGAAGCAGT ACCGCATGCT GAAGGCCCGC CGAGCCTACC GCAGGGTCTG CAGGGCTACC 
GTCATCATCC AGTCCTTCAC ACGGGCCATG TTTGTGCGCA GGAACTACCG CCAGGTCCTC 
ATGGAGCACA AAGCCACCAT CATCCAGAAG TACGCCCGAG GCTGGATGGC ACGGAAGCGT 
TTTCTGCGGG AGCGGGATGC AGCCATTGTC ATCCAGTGTG CCTTCCGGAG ACTCAAGGCC 
AGGCAGGAGC TGAAGGCCCT CAAGATTGAG GCCCGCTCGG CAGAGCATCT GAAACGCCTC 
AACGTGGGCA TGGAGAACAA AGTCGTCCAG CTACAGCGGA AGATTGATGA CCAGAACAAA 
GAGTTCAAGA CTCTGTCAGA GCAGCTGTCT GCAGTCACCT CCAGTCATGC CGTGGAGGTG 
GAGAAGCTGA AGAAGGAGCT GGCACATTAC CAGCAGAACC AGGAGGCTGA TACCAGCCTT 
CAGCTGCAGG AGGAGGTTCA GAGCCTGCGC ACTGAACTAC AGAAGGCTCA TTCGGAGCGC 
AGGGTCCTGG AGGATGCTCA CAACAAGGAG AATGGCGAGC TGAGAAAGCG AGTTGCAGAC 
CTGGAACATG AAAATGCCCT CTTGAAGGAT GAAAAAGAAT ACCTTAACAA CCAAATCCTG 
TGCCAGTCAA AAGCTGAATC TTCGCAGAGC TCTGTGGAAG AGAACCTCCT GATGAAGAAG 
GAGCTGGAGG AGGAACGGTC CAGGTACCAG AACCTCGTGA AGGAGTACTC CCAGCTGGAG 
CAGAGATATG AGAACCTTCG GGACGAGGTG ACGATCCTAA AGCAAACTCC GGGCCACAGG 
AAGAACCCAT CGAACCAAAG CAGCTTAGAA TCTGACTCCA ATTACCCCTC CATTTCCACT 
TCCGAAATCG GAGACACTGA GGATGCCCTT CAGCAGGTGG AGGAGATTGG CATAGAGAAG 
GCAGCCATGG ACATGACCGT CTTCCTGAAG CTGCAGAAGA GAGTGCGCGA ACTTGAGCAG 
GAGAGGAAGA AGCTGCAGGC ACAGCTAGAA AAGGGACAGC AGGACAGCAA GAAAGGGCAG 
GTAGAACAAC AGAACAATGG CTTAGATGTG GACCAGGACG CAGATATAGC CTACAATAGT 
CTGAAGAGAC AGGAGCTTGA GTCAGAGAAC AAGAAGCTGA AGAATGACCT GAATGAGCTG 
AGGAAGGCTG TCGCTGACCA AGCCATGCAG GATAACTCCA CCCACAGCTC CCCAGACAGC 
TACAGCCTCC TCCTGAACCA GCTCAAGCTG GCCAATGAGG AGCTCGAGGT CCGCAAAGAG 
GAGGTGCTGA TCCTCAGGAC CCAGATCATG AATGCCGACC AGCGCCGCCT GTCTGGCAAG 
AACATGGAGC CGAACATCAA TGCCAGAACA AGTTGGCCCA ACAGTGAGAA GCACGTGGAC 
CAGGAAGACG CCATTGAGGC CTATCACGGG GTCTGCCAGA CAAACAGCCA GACTGAGGAT 
TGGGGATATT TGAATGAAGA TGGAGAACTC GGCTTGGCTT ACCAAGGCCT AAAGCAAGTT 
GCCAGGTTGC TGGAGGCCCA GCTGCAGGCC CAGAGCCTGG AGCATGAGGA GGAGGTGGAA 
CATCTCAAGG CCCAGGTGGA AGCCCTGAAA GAGGAGATGG ACAAACAGCA GCAGACCTTC 
TGCCAGACCC TGCTGCTCTC CCCAGAGGCC CAGGTAGAAT TTGGTGTCCA GCAGGAGATA 
TCCCGGCTGA CCAATGAGAA CCTGGATTTT AAGGAATTGG TGGAAAAGCT GGAGAAGAAT 
GAGAGGAAGC TGAAGAAGCA GCTGAAGATT TACATGAAGA AGGTCCAGGA CTTAGAAGCT 
GCTCAGGCGT TGGCACAGAG TGACAGGAGA CACCATGAAC TCACAAGACA GGTCACAGTC 
CAACGAAAAG AGAAGGACTT CCAAGGCATG CTGGAGTACC ACAAAGAGGA CGAAGCCCTC 
CTCATCCGGA ACCTGGTGAC AGACCTGAAG CCTCAGATGC TGTCGGGCAC CGTGCCCTGT 
CTGCCTGCAT ACATACTCTA TATGTGCATC AGGCACGCGG ATTACACCAA CGATGACCTC 
AAGGTGCACT CGTTGCTGAG CTCCACCATC AACGGCATTA AGAAAGTCCT CAAGAAGCAC 
AATGATGACT TTGAGATGAC GTCATTCTGG CTATCCAACA CCTGCCGCTT CCTTCACTGT 
CTGAAGCAAT ACAGTGGTGA TGAGGGTTTC ATGACACAGA ACACTGCGAA GCAGAATGAG 
CACTGTCTCA AGAACTTTGA CCTCACTGAA TACCGCCAGG TGCTAAGCGA CCTTTCCATT 
CAGATCTATC AGCAGCTCAT TAAAATCGCC GAGGGCTTGC TGCAGCCTAT GATAGTTTCT 
GCCATGTTGG AAAATGAGAG TATCCAGGGG CTGTCTGGTG TGAGACCAAC TGGTTACCGG 
AAGCGCTCCT CCAGCATGGT GGATGGAGAG AATTCGTATT GCCTGGAGGC CATCGTCCGC 
CAGATGAATT CTTTCCATAC AGTCCTGTGT GACCAGGGTC TGGACCCCGA GATTATCCTG 
CAGGTGTTCA AACAGCTCTT CTACATGATC AACGCTGTGA CTCTTAACAA CCTACTCCTG 
CGGAAAGACG CCTGCTCCTG GAGCACAGGC ATGCAACTCA GGTACAACAT AAGTCAACTG 
GAAGAGTGGC TTCGGGGCAA AAACCTTCAC CAAAGTGGAG CAGTTCAGAC CATGGAGCCC 
CTCATCCAGG CAGCCCAGCT CCTCCAGCTG AAGAAGAAAA CCCACGAGGA TGCTGAGGCC 
ATCTGCTCTC TGTGCACCTC CCTCAGCACC CAGCAGATTG TCAAAATTTT AAACCTCTAC 
ACTCCCTTGA ATGAATTTGA GGAACGGGTC ACAGTGTCCT TCATACGAAC AATCCAGGCT 
CAGCTACAAG AGAGGAATGA CCCTCAGCAG CTCCTGCTGG ACTCCAAGCA CGTGTTCCCA 
GTTCTGTTTC CATATAACCC ATCTGCTCTG ACCATGGACT CGATCCACAT CCCGGCCTGT 
CTCAACCTGG AGTTTCTCAA TGAAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5b ( XM_006525715.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525778.1 Mus musculus myosin VB (Myo5b), transcript variant X2, mRNA.

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CloneID	OMu54431
Clone ID Related Accession (Same CDS sequence)	XM_006525715.3 , XM_006525715.4
Accession Version	XM_006525715.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Vb isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525715.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAA GAGGTAGTAG GTCCTCTCTG CTGCACAATA GTACATGGCA GTACACAAGG 
GTCTGGATCC CTGACCCAGA TGAGGTGTGG CGCTCGGCTG AATTAACCAA GGACTACAAA 
GAAGGGGATA AGAGCCTGCA GCTCAGACTA GAAGATGACA CAATTCTAGA ATACCCAGTT 
GATGTCCAAA ATAACCAGGT GCCATTCTTG CGGAATCCAG ATATCTTAGT AGGAGAAAAC 
GACCTCACTG CCCTCAGCCA CCTCCACGAG CCCGCAGTTC TGCATAACTT AAAGGTCCGT 
TTCCTGGAAT CCAACCACAT CTACACTTAC TGTGGAATTG TGCTTGTTGC CATCAACCCC 
TACGAGCAGC TGCCAATCTA CGGACAGGAT GTCATCTATG CCTACAGTGG CCAAAACATG 
GGTGACATGG ACCCCCACAT CTTTGCTGTG GCAGAAGAAG CCTACAAGCA GATGGCCAGA 
GATGAAAAGA ACCAATCCAT CATAGTCAGC GGAGAGTCTG GAGCAGGCAA GACGGTGTCG 
GCCAAGTACG CCATGCGCTA TTTTGCCACC GTTGGTGGCT CAGCCAGTGA TACCAACATT 
GAAGAGAAGG TCCTAGCATC CAGCCCCATC ATGGAGGCCA TTGGGAACGC CAAGACTACT 
CGCAACGACA ACAGCAGCCG ATTTGGGAAA TTCATTGAGA TTGGCTTTGA TAAAAAGTAC 
CACATCATTG GGGCCAACAT GAGGACCTAC TTGCTGGAGA AGTCCAGGGT GGTCTTTCAG 
GCGGATGACG AGAGGAACTA CCACATCTTT TACCAGCTCT GTGCTGCTGC CAGCCTTCCT 
GAATTTAAAG AGCTCGCCCT AACGTGCGCA GAGGACTTTT TCTATACAGC CCATGGAGGA 
AACACGACCA TCGAGGGTGT GAATGATGCA GACGACTTTG AGAAGACTAG ACAAGCCCTC 
ACACTCCTGG GAGTGCGGGA TTCCCATCAA ATAAGCATCT TTAAGATAAT TGCTTCTATC 
TTGCACCTGG GAAGTGTGGA GATTCAGTCT GAGCGGGACG GGGATTCCTG CAGCATTTCA 
CCCCAGGATG AACACTTGAG CAACTTCTGC AGCCTGCTAG GAATAGAACA CAGCCAGATG 
GAGCACTGGC TGTGTCATCG GAAGCTGGTC ACCACCTCAG AGACCTACGT CAAGACCATG 
TCCCTGCAGC AAGTGGTCAA TGCACGTGAT GCCTTAGCCA AGCACATCTA TGCTCAGCTG 
TTCTCCTGGA TTGTGGAGCA CATCAACAAA GCCCTGCACA CCTCCCTTAA GCAGCACTCC 
TTCATCGGGG TCCTGGATAT CTATGGGTTT GAGACCTTCG AGATCAATAG CTTTGAACAG 
TTTTGTATCA ACTACGCCAA TGAAAAGCTC CAGCAACAGT TCAATTCGCA TGTGTTCAAG 
CTGGAGCAAG AAGAATATAT GAAGGAGCAG ATCCCGTGGA CTTTGATTGA CTTCTACGAT 
AACCAACCGT GTATAGACCT CATAGAGGCA AAGCTGGGCA TCCTGGACCT GTTGGATGAG 
GAGTGTAAGG TCCCCAAAGG AACTGATCAG AACTGGGCCC AGAAACTCTA CGAACGACAC 
TCCAACAGCC AGCACTTCCA GAAACCACGC ATGTCCAACA CGGCCTTCAT TGTCAACCAC 
TTTGCAGATA AGGTGGAGTA CCTTTCAGAT GGTTTTCTAG AGAAAAACAG GGATACAGTA 
TATGAAGAAC AGATCAACAT CCTGAAGGCC AGCAAGTTCC CATTAGTGGC TGATTTGTTC 
CACGATGACA AGGACTCTGC TCCAGCCACC AACACAGCTA AGAATCGATC ATCTTCAAAG 
ATCAACGTTC GCTCTTCCAG ACCCCTCATC AAAGTCCCTA ACAAGGAGCA CAAGAAATCC 
GTGGGCTACC AGTTTCGTAC TTCCCTAAAC CTGCTTATGG AGACCCTGAA TGCCACAACG 
CCACACTATG TCCGGTGCAT CAAGCCCAAC GATGAAAAGC TTCCCTTCCA CTTTGACCCC 
AAGAGAGCTG TACAGCAACT CCGAGCCTGC GGTGTGCTGG AGACCATTCG GATCAGCGCG 
GCAGGCTACC CATCCAGGTG GACCTACCAT GACTTCTTCA ACCGGTATCG GGTGTTGATG 
AAGAAGAGAG AGCTCACCAA CACCGACAAG AAGAATATCT GCAAGTCTGT CCTGGAGAGT 
CTCATCAAGG ACCCAGACAA GTTCCAGTTT GGCCGCACCA AGATCTTCTT CCGAGCAGGC 
CAGGTGGCCT ACCTGGAGAA GCTTCGGGCA GACAAGTTCC GGGAAGCTAC TATCATGATC 
CAGAAGTCGG TCAGGGGATG GCTGCAGAGA GTGAAGTACC GTAGGCTGAG GGCAGCTACC 
TTATCCCTGC AGAGGTTCTG CAGGGGATAC CTAGCCCGCA GGCTGGCCGA ACACTTGAGG 
AGAACCCGGG CCGCCATCGT GTTCCAGAAG CAGTACCGCA TGCTGAAGGC CCGCCGAGCC 
TACCGCAGGG TCTGCAGGGC TACCGTCATC ATCCAGTCCT TCACACGGGC CATGTTTGTG 
CGCAGGAACT ACCGCCAGGT CCTCATGGAG CACAAAGCCA CCATCATCCA GAAGTACGCC 
CGAGGCTGGA TGGCACGGAA GCGTTTTCTG CGGGAGCGGG ATGCAGCCAT TGTCATCCAG 
TGTGCCTTCC GGAGACTCAA GGCCAGGCAG GAGCTGAAGG CCCTCAAGAT TGAGGCCCGC 
TCGGCAGAGC ATCTGAAACG CCTCAACGTG GGCATGGAGA ACAAAGTCGT CCAGCTACAG 
CGGAAGATTG ATGACCAGAA CAAAGAGTTC AAGACTCTGT CAGAGCAGCT GTCTGCAGTC 
ACCTCCAGTC ATGCCGTGGA GGTGGAGAAG CTGAAGAAGG AGCTGGCACA TTACCAGCAG 
AACCAGGAGG CTGATACCAG CCTTCAGCTG CAGGAGGAGG TTCAGAGCCT GCGCACTGAA 
CTACAGAAGG CTCATTCGGA GCGCAGGGTC CTGGAGGATG CTCACAACAA GGAGAATGGC 
GAGCTGAGAA AGCGAGTTGC AGACCTGGAA CATGAAAATG CCCTCTTGAA GGATGAAAAA 
GAATACCTTA ACAACCAAAT CCTGTGCCAG TCAAAAGCTG AATCTTCGCA GAGCTCTGTG 
GAAGAGAACC TCCTGATGAA GAAGGAGCTG GAGGAGGAAC GGTCCAGGTA CCAGAACCTC 
GTGAAGGAGT ACTCCCAGCT GGAGCAGAGA TATGAGAACC TTCGGGACGA GGTGACGATC 
CTAAAGCAAA CTCCGGGCCA CAGGAAGAAC CCATCGAACC AAAGCAGCTT AGAATCTGAC 
TCCAATTACC CCTCCATTTC CACTTCCGAA ATCGGAGACA CTGAGGATGC CCTTCAGCAG 
GTGGAGGAGA TTGGCATAGA GAAGGCAGCC ATGGACATGA CCGTCTTCCT GAAGCTGCAG 
AAGAGAGTGC GCGAACTTGA GCAGGAGAGG AAGAAGCTGC AGGCACAGCT AGAAAAGGGA 
CAGCAGGACA GCAAGAAAGG GCAGGTAGAA CAACAGAACA ATGGCTTAGA TGTGGACCAG 
GACGCAGATA TAGCCTACAA TAGTCTGAAG AGACAGGAGC TTGAGTCAGA GAACAAGAAG 
CTGAAGAATG ACCTGAATGA GCTGAGGAAG GCTGTCGCTG ACCAAGCCAT GCAGGATAAC 
TCCACCCACA GCTCCCCAGA CAGCTACAGC CTCCTCCTGA ACCAGCTCAA GCTGGCCAAT 
GAGGAGCTCG AGGTCCGCAA AGAGGAGGTG CTGATCCTCA GGACCCAGAT CATGAATGCC 
GACCAGCGCC GCCTGTCTGG CAAGAACATG GAGCCGAACA TCAATGCCAG AACAAGTTGG 
CCCAACAGTG AGAAGCACGT GGACCAGGAA GACGCCATTG AGGCCTATCA CGGGGTCTGC 
CAGACAAACA GGTTGCTGGA GGCCCAGCTG CAGGCCCAGA GCCTGGAGCA TGAGGAGGAG 
GTGGAACATC TCAAGGCCCA GGTGGAAGCC CTGAAAGAGG AGATGGACAA ACAGCAGCAG 
ACCTTCTGCC AGACCCTGCT GCTCTCCCCA GAGGCCCAGG TAGAATTTGG TGTCCAGCAG 
GAGATATCCC GGCTGACCAA TGAGAACCTG GATTTTAAGG AATTGGTGGA AAAGCTGGAG 
AAGAATGAGA GGAAGCTGAA GAAGCAGCTG AAGATTTACA TGAAGAAGGT CCAGGACTTA 
GAAGCTGCTC AGGCGTTGGC ACAGAGTGAC AGGAGACACC ATGAACTCAC AAGACAGGTC 
ACAGTCCAAC GAAAAGAGAA GGACTTCCAA GGCATGCTGG AGTACCACAA AGAGGACGAA 
GCCCTCCTCA TCCGGAACCT GGTGACAGAC CTGAAGCCTC AGATGCTGTC GGGCACCGTG 
CCCTGTCTGC CTGCATACAT ACTCTATATG TGCATCAGGC ACGCGGATTA CACCAACGAT 
GACCTCAAGG TGCACTCGTT GCTGAGCTCC ACCATCAACG GCATTAAGAA AGTCCTCAAG 
AAGCACAATG ATGACTTTGA GATGACGTCA TTCTGGCTAT CCAACACCTG CCGCTTCCTT 
CACTGTCTGA AGCAATACAG TGGTGATGAG GGTTTCATGA CACAGAACAC TGCGAAGCAG 
AATGAGCACT GTCTCAAGAA CTTTGACCTC ACTGAATACC GCCAGGTGCT AAGCGACCTT 
TCCATTCAGA TCTATCAGCA GCTCATTAAA ATCGCCGAGG GCTTGCTGCA GCCTATGATA 
GTTTCTGCCA TGTTGGAAAA TGAGAGTATC CAGGGGCTGT CTGGTGTGAG ACCAACTGGT 
TACCGGAAGC GCTCCTCCAG CATGGTGGAT GGAGAGAATT CGTATTGCCT GGAGGCCATC 
GTCCGCCAGA TGAATTCTTT CCATACAGTC CTGTGTGACC AGGGTCTGGA CCCCGAGATT 
ATCCTGCAGG TGTTCAAACA GCTCTTCTAC ATGATCAACG CTGTGACTCT TAACAACCTA 
CTCCTGCGGA AAGACGCCTG CTCCTGGAGC ACAGGCATGC AACTCAGGTA CAACATAAGT 
CAACTGGAAG AGTGGCTTCG GGGCAAAAAC CTTCACCAAA GTGGAGCAGT TCAGACCATG 
GAGCCCCTCA TCCAGGCAGC CCAGCTCCTC CAGCTGAAGA AGAAAACCCA CGAGGATGCT 
GAGGCCATCT GCTCTCTGTG CACCTCCCTC AGCACCCAGC AGATTGTCAA AATTTTAAAC 
CTCTACACTC CCTTGAATGA ATTTGAGGAA CGGGTCACAG TGTCCTTCAT ACGAACAATC 
CAGGCTCAGC TACAAGAGAG GAATGACCCT CAGCAGCTCC TGCTGGACTC CAAGCACGTG 
TTCCCAGTTC TGTTTCCATA TAACCCATCT GCTCTGACCA TGGACTCGAT CCACATCCCG 
GCCTGTCTCA ACCTGGAGTT TCTCAATGAA GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525778.1
CDS	97..5592
Translation	MDPRGSRSSLLHNSTWQYTRVWIPDPDEVWRSAELTKDYKEGDKSLQLRLEDDTILEYPVDVQNNQVPFLRNPDILVGENDLTALSHLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKFIEIGFDKKYHIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTAHGGNTTIEGVNDADDFEKTRQALTLLGVRDSHQISIFKIIASILHLGSVEIQSERDGDSCSISPQDEHLSNFCSLLGIEHSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARDALAKHIYAQLFSWIVEHINKALHTSLKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYERHSNSQHFQKPRMSNTAFIVNHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDDKDSAPATNTAKNRSSSKINVRSSRPLIKVPNKEHKKSVGYQFRTSLNLLMETLNATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWTYHDFFNRYRVLMKKRELTNTDKKNICKSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFREATIMIQKSVRGWLQRVKYRRLRAATLSLQRFCRGYLARRLAEHLRRTRAAIVFQKQYRMLKARRAYRRVCRATVIIQSFTRAMFVRRNYRQVLMEHKATIIQKYARGWMARKRFLRERDAAIVIQCAFRRLKARQELKALKIEARSAEHLKRLNVGMENKVVQLQRKIDDQNKEFKTLSEQLSAVTSSHAVEVEKLKKELAHYQQNQEADTSLQLQEEVQSLRTELQKAHSERRVLEDAHNKENGELRKRVADLEHENALLKDEKEYLNNQILCQSKAESSQSSVEENLLMKKELEEERSRYQNLVKEYSQLEQRYENLRDEVTILKQTPGHRKNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGIEKAAMDMTVFLKLQKRVRELEQERKKLQAQLEKGQQDSKKGQVEQQNNGLDVDQDADIAYNSLKRQELESENKKLKNDLNELRKAVADQAMQDNSTHSSPDSYSLLLNQLKLANEELEVRKEEVLILRTQIMNADQRRLSGKNMEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNRLLEAQLQAQSLEHEEEVEHLKAQVEALKEEMDKQQQTFCQTLLLSPEAQVEFGVQQEISRLTNENLDFKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSDRRHHELTRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTDLKPQMLSGTVPCLPAYILYMCIRHADYTNDDLKVHSLLSSTINGIKKVLKKHNDDFEMTSFWLSNTCRFLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGLLQPMIVSAMLENESIQGLSGVRPTGYRKRSSSMVDGENSYCLEAIVRQMNSFHTVLCDQGLDPEIILQVFKQLFYMINAVTLNNLLLRKDACSWSTGMQLRYNISQLEEWLRGKNLHQSGAVQTMEPLIQAAQLLQLKKKTHEDAEAICSLCTSLSTQQIVKILNLYTPLNEFEERVTVSFIRTIQAQLQERNDPQQLLLDSKHVFPVLFPYNPSALTMDSIHIPACLNLEFLNEV

Target ORF information:

RefSeq Version	XM_006525715.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VB (Myo5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525715.3

ORF Insert Sequence:

ATGGATCCAA GAGGTAGTAG GTCCTCTCTG CTGCACAATA GTACATGGCA GTACACAAGG 
GTCTGGATCC CTGACCCAGA TGAGGTGTGG CGCTCGGCTG AATTAACCAA GGACTACAAA 
GAAGGGGATA AGAGCCTGCA GCTCAGACTA GAAGATGACA CAATTCTAGA ATACCCAGTT 
GATGTCCAAA ATAACCAGGT GCCATTCTTG CGGAATCCAG ATATCTTAGT AGGAGAAAAC 
GACCTCACTG CCCTCAGCCA CCTCCACGAG CCCGCAGTTC TGCATAACTT AAAGGTCCGT 
TTCCTGGAAT CCAACCACAT CTACACTTAC TGTGGAATTG TGCTTGTTGC CATCAACCCC 
TACGAGCAGC TGCCAATCTA CGGACAGGAT GTCATCTATG CCTACAGTGG CCAAAACATG 
GGTGACATGG ACCCCCACAT CTTTGCTGTG GCAGAAGAAG CCTACAAGCA GATGGCCAGA 
GATGAAAAGA ACCAATCCAT CATAGTCAGC GGAGAGTCTG GAGCAGGCAA GACGGTGTCG 
GCCAAGTACG CCATGCGCTA TTTTGCCACC GTTGGTGGCT CAGCCAGTGA TACCAACATT 
GAAGAGAAGG TCCTAGCATC CAGCCCCATC ATGGAGGCCA TTGGGAACGC CAAGACTACT 
CGCAACGACA ACAGCAGCCG ATTTGGGAAA TTCATTGAGA TTGGCTTTGA TAAAAAGTAC 
CACATCATTG GGGCCAACAT GAGGACCTAC TTGCTGGAGA AGTCCAGGGT GGTCTTTCAG 
GCGGATGACG AGAGGAACTA CCACATCTTT TACCAGCTCT GTGCTGCTGC CAGCCTTCCT 
GAATTTAAAG AGCTCGCCCT AACGTGCGCA GAGGACTTTT TCTATACAGC CCATGGAGGA 
AACACGACCA TCGAGGGTGT GAATGATGCA GACGACTTTG AGAAGACTAG ACAAGCCCTC 
ACACTCCTGG GAGTGCGGGA TTCCCATCAA ATAAGCATCT TTAAGATAAT TGCTTCTATC 
TTGCACCTGG GAAGTGTGGA GATTCAGTCT GAGCGGGACG GGGATTCCTG CAGCATTTCA 
CCCCAGGATG AACACTTGAG CAACTTCTGC AGCCTGCTAG GAATAGAACA CAGCCAGATG 
GAGCACTGGC TGTGTCATCG GAAGCTGGTC ACCACCTCAG AGACCTACGT CAAGACCATG 
TCCCTGCAGC AAGTGGTCAA TGCACGTGAT GCCTTAGCCA AGCACATCTA TGCTCAGCTG 
TTCTCCTGGA TTGTGGAGCA CATCAACAAA GCCCTGCACA CCTCCCTTAA GCAGCACTCC 
TTCATCGGGG TCCTGGATAT CTATGGGTTT GAGACCTTCG AGATCAATAG CTTTGAACAG 
TTTTGTATCA ACTACGCCAA TGAAAAGCTC CAGCAACAGT TCAATTCGCA TGTGTTCAAG 
CTGGAGCAAG AAGAATATAT GAAGGAGCAG ATCCCGTGGA CTTTGATTGA CTTCTACGAT 
AACCAACCGT GTATAGACCT CATAGAGGCA AAGCTGGGCA TCCTGGACCT GTTGGATGAG 
GAGTGTAAGG TCCCCAAAGG AACTGATCAG AACTGGGCCC AGAAACTCTA CGAACGACAC 
TCCAACAGCC AGCACTTCCA GAAACCACGC ATGTCCAACA CGGCCTTCAT TGTCAACCAC 
TTTGCAGATA AGGTGGAGTA CCTTTCAGAT GGTTTTCTAG AGAAAAACAG GGATACAGTA 
TATGAAGAAC AGATCAACAT CCTGAAGGCC AGCAAGTTCC CATTAGTGGC TGATTTGTTC 
CACGATGACA AGGACTCTGC TCCAGCCACC AACACAGCTA AGAATCGATC ATCTTCAAAG 
ATCAACGTTC GCTCTTCCAG ACCCCTCATC AAAGTCCCTA ACAAGGAGCA CAAGAAATCC 
GTGGGCTACC AGTTTCGTAC TTCCCTAAAC CTGCTTATGG AGACCCTGAA TGCCACAACG 
CCACACTATG TCCGGTGCAT CAAGCCCAAC GATGAAAAGC TTCCCTTCCA CTTTGACCCC 
AAGAGAGCTG TACAGCAACT CCGAGCCTGC GGTGTGCTGG AGACCATTCG GATCAGCGCG 
GCAGGCTACC CATCCAGGTG GACCTACCAT GACTTCTTCA ACCGGTATCG GGTGTTGATG 
AAGAAGAGAG AGCTCACCAA CACCGACAAG AAGAATATCT GCAAGTCTGT CCTGGAGAGT 
CTCATCAAGG ACCCAGACAA GTTCCAGTTT GGCCGCACCA AGATCTTCTT CCGAGCAGGC 
CAGGTGGCCT ACCTGGAGAA GCTTCGGGCA GACAAGTTCC GGGAAGCTAC TATCATGATC 
CAGAAGTCGG TCAGGGGATG GCTGCAGAGA GTGAAGTACC GTAGGCTGAG GGCAGCTACC 
TTATCCCTGC AGAGGTTCTG CAGGGGATAC CTAGCCCGCA GGCTGGCCGA ACACTTGAGG 
AGAACCCGGG CCGCCATCGT GTTCCAGAAG CAGTACCGCA TGCTGAAGGC CCGCCGAGCC 
TACCGCAGGG TCTGCAGGGC TACCGTCATC ATCCAGTCCT TCACACGGGC CATGTTTGTG 
CGCAGGAACT ACCGCCAGGT CCTCATGGAG CACAAAGCCA CCATCATCCA GAAGTACGCC 
CGAGGCTGGA TGGCACGGAA GCGTTTTCTG CGGGAGCGGG ATGCAGCCAT TGTCATCCAG 
TGTGCCTTCC GGAGACTCAA GGCCAGGCAG GAGCTGAAGG CCCTCAAGAT TGAGGCCCGC 
TCGGCAGAGC ATCTGAAACG CCTCAACGTG GGCATGGAGA ACAAAGTCGT CCAGCTACAG 
CGGAAGATTG ATGACCAGAA CAAAGAGTTC AAGACTCTGT CAGAGCAGCT GTCTGCAGTC 
ACCTCCAGTC ATGCCGTGGA GGTGGAGAAG CTGAAGAAGG AGCTGGCACA TTACCAGCAG 
AACCAGGAGG CTGATACCAG CCTTCAGCTG CAGGAGGAGG TTCAGAGCCT GCGCACTGAA 
CTACAGAAGG CTCATTCGGA GCGCAGGGTC CTGGAGGATG CTCACAACAA GGAGAATGGC 
GAGCTGAGAA AGCGAGTTGC AGACCTGGAA CATGAAAATG CCCTCTTGAA GGATGAAAAA 
GAATACCTTA ACAACCAAAT CCTGTGCCAG TCAAAAGCTG AATCTTCGCA GAGCTCTGTG 
GAAGAGAACC TCCTGATGAA GAAGGAGCTG GAGGAGGAAC GGTCCAGGTA CCAGAACCTC 
GTGAAGGAGT ACTCCCAGCT GGAGCAGAGA TATGAGAACC TTCGGGACGA GGTGACGATC 
CTAAAGCAAA CTCCGGGCCA CAGGAAGAAC CCATCGAACC AAAGCAGCTT AGAATCTGAC 
TCCAATTACC CCTCCATTTC CACTTCCGAA ATCGGAGACA CTGAGGATGC CCTTCAGCAG 
GTGGAGGAGA TTGGCATAGA GAAGGCAGCC ATGGACATGA CCGTCTTCCT GAAGCTGCAG 
AAGAGAGTGC GCGAACTTGA GCAGGAGAGG AAGAAGCTGC AGGCACAGCT AGAAAAGGGA 
CAGCAGGACA GCAAGAAAGG GCAGGTAGAA CAACAGAACA ATGGCTTAGA TGTGGACCAG 
GACGCAGATA TAGCCTACAA TAGTCTGAAG AGACAGGAGC TTGAGTCAGA GAACAAGAAG 
CTGAAGAATG ACCTGAATGA GCTGAGGAAG GCTGTCGCTG ACCAAGCCAT GCAGGATAAC 
TCCACCCACA GCTCCCCAGA CAGCTACAGC CTCCTCCTGA ACCAGCTCAA GCTGGCCAAT 
GAGGAGCTCG AGGTCCGCAA AGAGGAGGTG CTGATCCTCA GGACCCAGAT CATGAATGCC 
GACCAGCGCC GCCTGTCTGG CAAGAACATG GAGCCGAACA TCAATGCCAG AACAAGTTGG 
CCCAACAGTG AGAAGCACGT GGACCAGGAA GACGCCATTG AGGCCTATCA CGGGGTCTGC 
CAGACAAACA GGTTGCTGGA GGCCCAGCTG CAGGCCCAGA GCCTGGAGCA TGAGGAGGAG 
GTGGAACATC TCAAGGCCCA GGTGGAAGCC CTGAAAGAGG AGATGGACAA ACAGCAGCAG 
ACCTTCTGCC AGACCCTGCT GCTCTCCCCA GAGGCCCAGG TAGAATTTGG TGTCCAGCAG 
GAGATATCCC GGCTGACCAA TGAGAACCTG GATTTTAAGG AATTGGTGGA AAAGCTGGAG 
AAGAATGAGA GGAAGCTGAA GAAGCAGCTG AAGATTTACA TGAAGAAGGT CCAGGACTTA 
GAAGCTGCTC AGGCGTTGGC ACAGAGTGAC AGGAGACACC ATGAACTCAC AAGACAGGTC 
ACAGTCCAAC GAAAAGAGAA GGACTTCCAA GGCATGCTGG AGTACCACAA AGAGGACGAA 
GCCCTCCTCA TCCGGAACCT GGTGACAGAC CTGAAGCCTC AGATGCTGTC GGGCACCGTG 
CCCTGTCTGC CTGCATACAT ACTCTATATG TGCATCAGGC ACGCGGATTA CACCAACGAT 
GACCTCAAGG TGCACTCGTT GCTGAGCTCC ACCATCAACG GCATTAAGAA AGTCCTCAAG 
AAGCACAATG ATGACTTTGA GATGACGTCA TTCTGGCTAT CCAACACCTG CCGCTTCCTT 
CACTGTCTGA AGCAATACAG TGGTGATGAG GGTTTCATGA CACAGAACAC TGCGAAGCAG 
AATGAGCACT GTCTCAAGAA CTTTGACCTC ACTGAATACC GCCAGGTGCT AAGCGACCTT 
TCCATTCAGA TCTATCAGCA GCTCATTAAA ATCGCCGAGG GCTTGCTGCA GCCTATGATA 
GTTTCTGCCA TGTTGGAAAA TGAGAGTATC CAGGGGCTGT CTGGTGTGAG ACCAACTGGT 
TACCGGAAGC GCTCCTCCAG CATGGTGGAT GGAGAGAATT CGTATTGCCT GGAGGCCATC 
GTCCGCCAGA TGAATTCTTT CCATACAGTC CTGTGTGACC AGGGTCTGGA CCCCGAGATT 
ATCCTGCAGG TGTTCAAACA GCTCTTCTAC ATGATCAACG CTGTGACTCT TAACAACCTA 
CTCCTGCGGA AAGACGCCTG CTCCTGGAGC ACAGGCATGC AACTCAGGTA CAACATAAGT 
CAACTGGAAG AGTGGCTTCG GGGCAAAAAC CTTCACCAAA GTGGAGCAGT TCAGACCATG 
GAGCCCCTCA TCCAGGCAGC CCAGCTCCTC CAGCTGAAGA AGAAAACCCA CGAGGATGCT 
GAGGCCATCT GCTCTCTGTG CACCTCCCTC AGCACCCAGC AGATTGTCAA AATTTTAAAC 
CTCTACACTC CCTTGAATGA ATTTGAGGAA CGGGTCACAG TGTCCTTCAT ACGAACAATC 
CAGGCTCAGC TACAAGAGAG GAATGACCCT CAGCAGCTCC TGCTGGACTC CAAGCACGTG 
TTCCCAGTTC TGTTTCCATA TAACCCATCT GCTCTGACCA TGGACTCGAT CCACATCCCG 
GCCTGTCTCA ACCTGGAGTT TCTCAATGAA GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5b ( XM_006525716.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525779.1 Mus musculus myosin VB (Myo5b), transcript variant X3, mRNA.

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CloneID	OMu54432
Clone ID Related Accession (Same CDS sequence)	XM_006525716.3
Accession Version	XM_006525716.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Vb isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525716.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAA GAGGTAGTAG GTCCTCTCTG CTGCACAATA GTACATGGCA GTACACAAGG 
GTCTGGATCC CTGACCCAGA TGAGGTGTGG CGCTCGGCTG AATTAACCAA GGACTACAAA 
GAAGGGGATA AGAGCCTGCA GCTCAGACTA GAAGATGACA CAATTCTAGA ATACCCAGTT 
GATGTCCAAA ATAACCAGGT GCCATTCTTG CGGAATCCAG ATATCTTAGT AGGAGAAAAC 
GACCTCACTG CCCTCAGCCA CCTCCACGAG CCCGCAGTTC TGCATAACTT AAAGGTCCGT 
TTCCTGGAAT CCAACCACAT CTACACTTAC TGTGGAATTG TGCTTGTTGC CATCAACCCC 
TACGAGCAGC TGCCAATCTA CGGACAGGAT GTCATCTATG CCTACAGTGG CCAAAACATG 
GGTGACATGG ACCCCCACAT CTTTGCTGTG GCAGAAGAAG CCTACAAGCA GATGGCCAGA 
GATGAAAAGA ACCAATCCAT CATAGTCAGC GGAGAGTCTG GAGCAGGCAA GACGGTGTCG 
GCCAAGTACG CCATGCGCTA TTTTGCCACC GTTGGTGGCT CAGCCAGTGA TACCAACATT 
GAAGAGAAGG TCCTAGCATC CAGCCCCATC ATGGAGGCCA TTGGGAACGC CAAGACTACT 
CGCAACGACA ACAGCAGCCG ATTTGGGAAA TTCATTGAGA TTGGCTTTGA TAAAAAGTAC 
CACATCATTG GGGCCAACAT GAGGACCTAC TTGCTGGAGA AGTCCAGGGT GGTCTTTCAG 
GCGGATGACG AGAGGAACTA CCACATCTTT TACCAGCTCT GTGCTGCTGC CAGCCTTCCT 
GAATTTAAAG AGCTCGCCCT AACGTGCGCA GAGGACTTTT TCTATACAGC CCATGGAGGA 
AACACGACCA TCGAGGGTGT GAATGATGCA GACGACTTTG AGAAGACTAG ACAAGCCCTC 
ACACTCCTGG GAGTGCGGGA TTCCCATCAA ATAAGCATCT TTAAGATAAT TGCTTCTATC 
TTGCACCTGG GAAGTGTGGA GATTCAGTCT GAGCGGGACG GGGATTCCTG CAGCATTTCA 
CCCCAGGATG AACACTTGAG CAACTTCTGC AGCCTGCTAG GAATAGAACA CAGCCAGATG 
GAGCACTGGC TGTGTCATCG GAAGCTGGTC ACCACCTCAG AGACCTACGT CAAGACCATG 
TCCCTGCAGC AAGTGGTCAA TGCACGTGAT GCCTTAGCCA AGCACATCTA TGCTCAGCTG 
TTCTCCTGGA TTGTGGAGCA CATCAACAAA GCCCTGCACA CCTCCCTTAA GCAGCACTCC 
TTCATCGGGG TCCTGGATAT CTATGGGTTT GAGACCTTCG AGATCAATAG CTTTGAACAG 
TTTTGTATCA ACTACGCCAA TGAAAAGCTC CAGCAACAGT TCAATTCGCA TGTGTTCAAG 
CTGGAGCAAG AAGAATATAT GAAGGAGCAG ATCCCGTGGA CTTTGATTGA CTTCTACGAT 
AACCAACCGT GTATAGACCT CATAGAGGCA AAGCTGGGCA TCCTGGACCT GTTGGATGAG 
GAGTGTAAGG TCCCCAAAGG AACTGATCAG AACTGGGCCC AGAAACTCTA CGAACGACAC 
TCCAACAGCC AGCACTTCCA GAAACCACGC ATGTCCAACA CGGCCTTCAT TGTCAACCAC 
TTTGCAGATA AGGTGGAGTA CCTTTCAGAT GGTTTTCTAG AGAAAAACAG GGATACAGTA 
TATGAAGAAC AGATCAACAT CCTGAAGGCC AGCAAGTTCC CATTAGTGGC TGATTTGTTC 
CACGATGACA AGGACTCTGC TCCAGCCACC AACACAGCTA AGAATCGATC ATCTTCAAAG 
ATCAACGTTC GCTCTTCCAG ACCCCTCATC AAAGTCCCTA ACAAGGAGCA CAAGAAATCC 
GTGGGCTACC AGTTTCGTAC TTCCCTAAAC CTGCTTATGG AGACCCTGAA TGCCACAACG 
CCACACTATG TCCGGTGCAT CAAGCCCAAC GATGAAAAGC TTCCCTTCCA CTTTGACCCC 
AAGAGAGCTG TACAGCAACT CCGAGCCTGC GGTGTGCTGG AGACCATTCG GATCAGCGCG 
GCAGGCTACC CATCCAGGTG GACCTACCAT GACTTCTTCA ACCGGTATCG GGTGTTGATG 
AAGAAGAGAG AGCTCACCAA CACCGACAAG AAGAATATCT GCAAGTCTGT CCTGGAGAGT 
CTCATCAAGG ACCCAGACAA GTTCCAGTTT GGCCGCACCA AGATCTTCTT CCGAGCAGGC 
CAGGTGGCCT ACCTGGAGAA GCTTCGGGCA GACAAGTTCC GGGAAGCTAC TATCATGATC 
CAGAAGTCGG TCAGGGGATG GCTGCAGAGA GTGAAGTACC GTAGGCTGAG GGCAGCTACC 
TTATCCCTGC AGAGGTTCTG CAGGGGATAC CTAGCCCGCA GGCTGGCCGA ACACTTGAGG 
AGAACCCGGG CCGCCATCGT GTTCCAGAAG CAGTACCGCA TGCTGAAGGC CCGCCGAGCC 
TACCGCAGGG TCTGCAGGGC TACCGTCATC ATCCAGTCCT TCACACGGGC CATGTTTGTG 
CGCAGGAACT ACCGCCAGGT CCTCATGGAG CACAAAGCCA CCATCATCCA GAAGTACGCC 
CGAGGCTGGA TGGCACGGAA GCGTTTTCTG CGGGAGCGGG ATGCAGCCAT TGTCATCCAG 
TGTGCCTTCC GGAGACTCAA GGCCAGGCAG GAGCTGAAGG CCCTCAAGAT TGAGGCCCGC 
TCGGCAGAGC ATCTGAAACG CCTCAACGTG GGCATGGAGA ACAAAGTCGT CCAGCTACAG 
CGGAAGATTG ATGACCAGAA CAAAGAGTTC AAGACTCTGT CAGAGCAGCT GTCTGCAGTC 
ACCTCCAGTC ATGCCGTGGA GGTGGAGAAG CTGAAGAAGG AGCTGGCACA TTACCAGCAG 
AACCAGGAGG CTGATACCAG CCTTCAGCTG CAGGAGGAGG TTCAGAGCCT GCGCACTGAA 
CTACAGAAGG CTCATTCGGA GCGCAGGGTC CTGGAGGATG CTCACAACAA GGAGAATGGC 
GAGCTGAGAA AGCGAGTTGC AGACCTGGAA CATGAAAATG CCCTCTTGAA GGATGAAAAA 
GAATACCTTA ACAACCAAAT CCTGTGCCAG TCAAAAGCTG AATCTTCGCA GAGCTCTGTG 
GAAGAGAACC TCCTGATGAA GAAGGAGCTG GAGGAGGAAC GGTCCAGGTA CCAGAACCTC 
GTGAAGGAGT ACTCCCAGCT GGAGCAGAGA TATGAGAACC TTCGGGACGA GCAAACTCCG 
GGCCACAGGA AGAACCCATC GAACCAAAGC AGCTTAGAAT CTGACTCCAA TTACCCCTCC 
ATTTCCACTT CCGAAATCGG AGACACTGAG GATGCCCTTC AGCAGGTGGA GGAGATTGGC 
ATAGAGAAGG CAGCCATGGA CATGACCGTC TTCCTGAAGC TGCAGAAGAG AGTGCGCGAA 
CTTGAGCAGG AGAGGAAGAA GCTGCAGGCA CAGCTAGAAA AGGGACAGCA GGACAGCAAG 
AAAGGGCAGG TAGAACAACA GAACAATGGC TTAGATGTGG ACCAGGACGC AGATATAGCC 
TACAATAGTC TGAAGAGACA GGAGCTTGAG TCAGAGAACA AGAAGCTGAA GAATGACCTG 
AATGAGCTGA GGAAGGCTGT CGCTGACCAA GCCATGCAGG ATAACTCCAC CCACAGCTCC 
CCAGACAGCT ACAGCCTCCT CCTGAACCAG CTCAAGCTGG CCAATGAGGA GCTCGAGGTC 
CGCAAAGAGG AGGTGCTGAT CCTCAGGACC CAGATCATGA ATGCCGACCA GCGCCGCCTG 
TCTGGCAAGA ACATGGAGCC GAACATCAAT GCCAGAACAA GTTGGCCCAA CAGTGAGAAG 
CACGTGGACC AGGAAGACGC CATTGAGGCC TATCACGGGG TCTGCCAGAC AAACAGGTTG 
CTGGAGGCCC AGCTGCAGGC CCAGAGCCTG GAGCATGAGG AGGAGGTGGA ACATCTCAAG 
GCCCAGGTGG AAGCCCTGAA AGAGGAGATG GACAAACAGC AGCAGACCTT CTGCCAGACC 
CTGCTGCTCT CCCCAGAGGC CCAGGTAGAA TTTGGTGTCC AGCAGGAGAT ATCCCGGCTG 
ACCAATGAGA ACCTGGATTT TAAGGAATTG GTGGAAAAGC TGGAGAAGAA TGAGAGGAAG 
CTGAAGAAGC AGCTGAAGAT TTACATGAAG AAGGTCCAGG ACTTAGAAGC TGCTCAGGCG 
TTGGCACAGA GTGACAGGAG ACACCATGAA CTCACAAGAC AGGTCACAGT CCAACGAAAA 
GAGAAGGACT TCCAAGGCAT GCTGGAGTAC CACAAAGAGG ACGAAGCCCT CCTCATCCGG 
AACCTGGTGA CAGACCTGAA GCCTCAGATG CTGTCGGGCA CCGTGCCCTG TCTGCCTGCA 
TACATACTCT ATATGTGCAT CAGGCACGCG GATTACACCA ACGATGACCT CAAGGTGCAC 
TCGTTGCTGA GCTCCACCAT CAACGGCATT AAGAAAGTCC TCAAGAAGCA CAATGATGAC 
TTTGAGATGA CGTCATTCTG GCTATCCAAC ACCTGCCGCT TCCTTCACTG TCTGAAGCAA 
TACAGTGGTG ATGAGGGTTT CATGACACAG AACACTGCGA AGCAGAATGA GCACTGTCTC 
AAGAACTTTG ACCTCACTGA ATACCGCCAG GTGCTAAGCG ACCTTTCCAT TCAGATCTAT 
CAGCAGCTCA TTAAAATCGC CGAGGGCTTG CTGCAGCCTA TGATAGTTTC TGCCATGTTG 
GAAAATGAGA GTATCCAGGG GCTGTCTGGT GTGAGACCAA CTGGTTACCG GAAGCGCTCC 
TCCAGCATGG TGGATGGAGA GAATTCGTAT TGCCTGGAGG CCATCGTCCG CCAGATGAAT 
TCTTTCCATA CAGTCCTGTG TGACCAGGGT CTGGACCCCG AGATTATCCT GCAGGTGTTC 
AAACAGCTCT TCTACATGAT CAACGCTGTG ACTCTTAACA ACCTACTCCT GCGGAAAGAC 
GCCTGCTCCT GGAGCACAGG CATGCAACTC AGGTACAACA TAAGTCAACT GGAAGAGTGG 
CTTCGGGGCA AAAACCTTCA CCAAAGTGGA GCAGTTCAGA CCATGGAGCC CCTCATCCAG 
GCAGCCCAGC TCCTCCAGCT GAAGAAGAAA ACCCACGAGG ATGCTGAGGC CATCTGCTCT 
CTGTGCACCT CCCTCAGCAC CCAGCAGATT GTCAAAATTT TAAACCTCTA CACTCCCTTG 
AATGAATTTG AGGAACGGGT CACAGTGTCC TTCATACGAA CAATCCAGGC TCAGCTACAA 
GAGAGGAATG ACCCTCAGCA GCTCCTGCTG GACTCCAAGC ACGTGTTCCC AGTTCTGTTT 
CCATATAACC CATCTGCTCT GACCATGGAC TCGATCCACA TCCCGGCCTG TCTCAACCTG 
GAGTTTCTCA ATGAAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525779.1
CDS	96..5576
Translation	MDPRGSRSSLLHNSTWQYTRVWIPDPDEVWRSAELTKDYKEGDKSLQLRLEDDTILEYPVDVQNNQVPFLRNPDILVGENDLTALSHLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKFIEIGFDKKYHIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTAHGGNTTIEGVNDADDFEKTRQALTLLGVRDSHQISIFKIIASILHLGSVEIQSERDGDSCSISPQDEHLSNFCSLLGIEHSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARDALAKHIYAQLFSWIVEHINKALHTSLKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYERHSNSQHFQKPRMSNTAFIVNHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDDKDSAPATNTAKNRSSSKINVRSSRPLIKVPNKEHKKSVGYQFRTSLNLLMETLNATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWTYHDFFNRYRVLMKKRELTNTDKKNICKSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFREATIMIQKSVRGWLQRVKYRRLRAATLSLQRFCRGYLARRLAEHLRRTRAAIVFQKQYRMLKARRAYRRVCRATVIIQSFTRAMFVRRNYRQVLMEHKATIIQKYARGWMARKRFLRERDAAIVIQCAFRRLKARQELKALKIEARSAEHLKRLNVGMENKVVQLQRKIDDQNKEFKTLSEQLSAVTSSHAVEVEKLKKELAHYQQNQEADTSLQLQEEVQSLRTELQKAHSERRVLEDAHNKENGELRKRVADLEHENALLKDEKEYLNNQILCQSKAESSQSSVEENLLMKKELEEERSRYQNLVKEYSQLEQRYENLRDEQTPGHRKNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGIEKAAMDMTVFLKLQKRVRELEQERKKLQAQLEKGQQDSKKGQVEQQNNGLDVDQDADIAYNSLKRQELESENKKLKNDLNELRKAVADQAMQDNSTHSSPDSYSLLLNQLKLANEELEVRKEEVLILRTQIMNADQRRLSGKNMEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNRLLEAQLQAQSLEHEEEVEHLKAQVEALKEEMDKQQQTFCQTLLLSPEAQVEFGVQQEISRLTNENLDFKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSDRRHHELTRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTDLKPQMLSGTVPCLPAYILYMCIRHADYTNDDLKVHSLLSSTINGIKKVLKKHNDDFEMTSFWLSNTCRFLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGLLQPMIVSAMLENESIQGLSGVRPTGYRKRSSSMVDGENSYCLEAIVRQMNSFHTVLCDQGLDPEIILQVFKQLFYMINAVTLNNLLLRKDACSWSTGMQLRYNISQLEEWLRGKNLHQSGAVQTMEPLIQAAQLLQLKKKTHEDAEAICSLCTSLSTQQIVKILNLYTPLNEFEERVTVSFIRTIQAQLQERNDPQQLLLDSKHVFPVLFPYNPSALTMDSIHIPACLNLEFLNEV

Target ORF information:

RefSeq Version	XM_006525716.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VB (Myo5b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525716.3

ORF Insert Sequence:

ATGGATCCAA GAGGTAGTAG GTCCTCTCTG CTGCACAATA GTACATGGCA GTACACAAGG 
GTCTGGATCC CTGACCCAGA TGAGGTGTGG CGCTCGGCTG AATTAACCAA GGACTACAAA 
GAAGGGGATA AGAGCCTGCA GCTCAGACTA GAAGATGACA CAATTCTAGA ATACCCAGTT 
GATGTCCAAA ATAACCAGGT GCCATTCTTG CGGAATCCAG ATATCTTAGT AGGAGAAAAC 
GACCTCACTG CCCTCAGCCA CCTCCACGAG CCCGCAGTTC TGCATAACTT AAAGGTCCGT 
TTCCTGGAAT CCAACCACAT CTACACTTAC TGTGGAATTG TGCTTGTTGC CATCAACCCC 
TACGAGCAGC TGCCAATCTA CGGACAGGAT GTCATCTATG CCTACAGTGG CCAAAACATG 
GGTGACATGG ACCCCCACAT CTTTGCTGTG GCAGAAGAAG CCTACAAGCA GATGGCCAGA 
GATGAAAAGA ACCAATCCAT CATAGTCAGC GGAGAGTCTG GAGCAGGCAA GACGGTGTCG 
GCCAAGTACG CCATGCGCTA TTTTGCCACC GTTGGTGGCT CAGCCAGTGA TACCAACATT 
GAAGAGAAGG TCCTAGCATC CAGCCCCATC ATGGAGGCCA TTGGGAACGC CAAGACTACT 
CGCAACGACA ACAGCAGCCG ATTTGGGAAA TTCATTGAGA TTGGCTTTGA TAAAAAGTAC 
CACATCATTG GGGCCAACAT GAGGACCTAC TTGCTGGAGA AGTCCAGGGT GGTCTTTCAG 
GCGGATGACG AGAGGAACTA CCACATCTTT TACCAGCTCT GTGCTGCTGC CAGCCTTCCT 
GAATTTAAAG AGCTCGCCCT AACGTGCGCA GAGGACTTTT TCTATACAGC CCATGGAGGA 
AACACGACCA TCGAGGGTGT GAATGATGCA GACGACTTTG AGAAGACTAG ACAAGCCCTC 
ACACTCCTGG GAGTGCGGGA TTCCCATCAA ATAAGCATCT TTAAGATAAT TGCTTCTATC 
TTGCACCTGG GAAGTGTGGA GATTCAGTCT GAGCGGGACG GGGATTCCTG CAGCATTTCA 
CCCCAGGATG AACACTTGAG CAACTTCTGC AGCCTGCTAG GAATAGAACA CAGCCAGATG 
GAGCACTGGC TGTGTCATCG GAAGCTGGTC ACCACCTCAG AGACCTACGT CAAGACCATG 
TCCCTGCAGC AAGTGGTCAA TGCACGTGAT GCCTTAGCCA AGCACATCTA TGCTCAGCTG 
TTCTCCTGGA TTGTGGAGCA CATCAACAAA GCCCTGCACA CCTCCCTTAA GCAGCACTCC 
TTCATCGGGG TCCTGGATAT CTATGGGTTT GAGACCTTCG AGATCAATAG CTTTGAACAG 
TTTTGTATCA ACTACGCCAA TGAAAAGCTC CAGCAACAGT TCAATTCGCA TGTGTTCAAG 
CTGGAGCAAG AAGAATATAT GAAGGAGCAG ATCCCGTGGA CTTTGATTGA CTTCTACGAT 
AACCAACCGT GTATAGACCT CATAGAGGCA AAGCTGGGCA TCCTGGACCT GTTGGATGAG 
GAGTGTAAGG TCCCCAAAGG AACTGATCAG AACTGGGCCC AGAAACTCTA CGAACGACAC 
TCCAACAGCC AGCACTTCCA GAAACCACGC ATGTCCAACA CGGCCTTCAT TGTCAACCAC 
TTTGCAGATA AGGTGGAGTA CCTTTCAGAT GGTTTTCTAG AGAAAAACAG GGATACAGTA 
TATGAAGAAC AGATCAACAT CCTGAAGGCC AGCAAGTTCC CATTAGTGGC TGATTTGTTC 
CACGATGACA AGGACTCTGC TCCAGCCACC AACACAGCTA AGAATCGATC ATCTTCAAAG 
ATCAACGTTC GCTCTTCCAG ACCCCTCATC AAAGTCCCTA ACAAGGAGCA CAAGAAATCC 
GTGGGCTACC AGTTTCGTAC TTCCCTAAAC CTGCTTATGG AGACCCTGAA TGCCACAACG 
CCACACTATG TCCGGTGCAT CAAGCCCAAC GATGAAAAGC TTCCCTTCCA CTTTGACCCC 
AAGAGAGCTG TACAGCAACT CCGAGCCTGC GGTGTGCTGG AGACCATTCG GATCAGCGCG 
GCAGGCTACC CATCCAGGTG GACCTACCAT GACTTCTTCA ACCGGTATCG GGTGTTGATG 
AAGAAGAGAG AGCTCACCAA CACCGACAAG AAGAATATCT GCAAGTCTGT CCTGGAGAGT 
CTCATCAAGG ACCCAGACAA GTTCCAGTTT GGCCGCACCA AGATCTTCTT CCGAGCAGGC 
CAGGTGGCCT ACCTGGAGAA GCTTCGGGCA GACAAGTTCC GGGAAGCTAC TATCATGATC 
CAGAAGTCGG TCAGGGGATG GCTGCAGAGA GTGAAGTACC GTAGGCTGAG GGCAGCTACC 
TTATCCCTGC AGAGGTTCTG CAGGGGATAC CTAGCCCGCA GGCTGGCCGA ACACTTGAGG 
AGAACCCGGG CCGCCATCGT GTTCCAGAAG CAGTACCGCA TGCTGAAGGC CCGCCGAGCC 
TACCGCAGGG TCTGCAGGGC TACCGTCATC ATCCAGTCCT TCACACGGGC CATGTTTGTG 
CGCAGGAACT ACCGCCAGGT CCTCATGGAG CACAAAGCCA CCATCATCCA GAAGTACGCC 
CGAGGCTGGA TGGCACGGAA GCGTTTTCTG CGGGAGCGGG ATGCAGCCAT TGTCATCCAG 
TGTGCCTTCC GGAGACTCAA GGCCAGGCAG GAGCTGAAGG CCCTCAAGAT TGAGGCCCGC 
TCGGCAGAGC ATCTGAAACG CCTCAACGTG GGCATGGAGA ACAAAGTCGT CCAGCTACAG 
CGGAAGATTG ATGACCAGAA CAAAGAGTTC AAGACTCTGT CAGAGCAGCT GTCTGCAGTC 
ACCTCCAGTC ATGCCGTGGA GGTGGAGAAG CTGAAGAAGG AGCTGGCACA TTACCAGCAG 
AACCAGGAGG CTGATACCAG CCTTCAGCTG CAGGAGGAGG TTCAGAGCCT GCGCACTGAA 
CTACAGAAGG CTCATTCGGA GCGCAGGGTC CTGGAGGATG CTCACAACAA GGAGAATGGC 
GAGCTGAGAA AGCGAGTTGC AGACCTGGAA CATGAAAATG CCCTCTTGAA GGATGAAAAA 
GAATACCTTA ACAACCAAAT CCTGTGCCAG TCAAAAGCTG AATCTTCGCA GAGCTCTGTG 
GAAGAGAACC TCCTGATGAA GAAGGAGCTG GAGGAGGAAC GGTCCAGGTA CCAGAACCTC 
GTGAAGGAGT ACTCCCAGCT GGAGCAGAGA TATGAGAACC TTCGGGACGA GCAAACTCCG 
GGCCACAGGA AGAACCCATC GAACCAAAGC AGCTTAGAAT CTGACTCCAA TTACCCCTCC 
ATTTCCACTT CCGAAATCGG AGACACTGAG GATGCCCTTC AGCAGGTGGA GGAGATTGGC 
ATAGAGAAGG CAGCCATGGA CATGACCGTC TTCCTGAAGC TGCAGAAGAG AGTGCGCGAA 
CTTGAGCAGG AGAGGAAGAA GCTGCAGGCA CAGCTAGAAA AGGGACAGCA GGACAGCAAG 
AAAGGGCAGG TAGAACAACA GAACAATGGC TTAGATGTGG ACCAGGACGC AGATATAGCC 
TACAATAGTC TGAAGAGACA GGAGCTTGAG TCAGAGAACA AGAAGCTGAA GAATGACCTG 
AATGAGCTGA GGAAGGCTGT CGCTGACCAA GCCATGCAGG ATAACTCCAC CCACAGCTCC 
CCAGACAGCT ACAGCCTCCT CCTGAACCAG CTCAAGCTGG CCAATGAGGA GCTCGAGGTC 
CGCAAAGAGG AGGTGCTGAT CCTCAGGACC CAGATCATGA ATGCCGACCA GCGCCGCCTG 
TCTGGCAAGA ACATGGAGCC GAACATCAAT GCCAGAACAA GTTGGCCCAA CAGTGAGAAG 
CACGTGGACC AGGAAGACGC CATTGAGGCC TATCACGGGG TCTGCCAGAC AAACAGGTTG 
CTGGAGGCCC AGCTGCAGGC CCAGAGCCTG GAGCATGAGG AGGAGGTGGA ACATCTCAAG 
GCCCAGGTGG AAGCCCTGAA AGAGGAGATG GACAAACAGC AGCAGACCTT CTGCCAGACC 
CTGCTGCTCT CCCCAGAGGC CCAGGTAGAA TTTGGTGTCC AGCAGGAGAT ATCCCGGCTG 
ACCAATGAGA ACCTGGATTT TAAGGAATTG GTGGAAAAGC TGGAGAAGAA TGAGAGGAAG 
CTGAAGAAGC AGCTGAAGAT TTACATGAAG AAGGTCCAGG ACTTAGAAGC TGCTCAGGCG 
TTGGCACAGA GTGACAGGAG ACACCATGAA CTCACAAGAC AGGTCACAGT CCAACGAAAA 
GAGAAGGACT TCCAAGGCAT GCTGGAGTAC CACAAAGAGG ACGAAGCCCT CCTCATCCGG 
AACCTGGTGA CAGACCTGAA GCCTCAGATG CTGTCGGGCA CCGTGCCCTG TCTGCCTGCA 
TACATACTCT ATATGTGCAT CAGGCACGCG GATTACACCA ACGATGACCT CAAGGTGCAC 
TCGTTGCTGA GCTCCACCAT CAACGGCATT AAGAAAGTCC TCAAGAAGCA CAATGATGAC 
TTTGAGATGA CGTCATTCTG GCTATCCAAC ACCTGCCGCT TCCTTCACTG TCTGAAGCAA 
TACAGTGGTG ATGAGGGTTT CATGACACAG AACACTGCGA AGCAGAATGA GCACTGTCTC 
AAGAACTTTG ACCTCACTGA ATACCGCCAG GTGCTAAGCG ACCTTTCCAT TCAGATCTAT 
CAGCAGCTCA TTAAAATCGC CGAGGGCTTG CTGCAGCCTA TGATAGTTTC TGCCATGTTG 
GAAAATGAGA GTATCCAGGG GCTGTCTGGT GTGAGACCAA CTGGTTACCG GAAGCGCTCC 
TCCAGCATGG TGGATGGAGA GAATTCGTAT TGCCTGGAGG CCATCGTCCG CCAGATGAAT 
TCTTTCCATA CAGTCCTGTG TGACCAGGGT CTGGACCCCG AGATTATCCT GCAGGTGTTC 
AAACAGCTCT TCTACATGAT CAACGCTGTG ACTCTTAACA ACCTACTCCT GCGGAAAGAC 
GCCTGCTCCT GGAGCACAGG CATGCAACTC AGGTACAACA TAAGTCAACT GGAAGAGTGG 
CTTCGGGGCA AAAACCTTCA CCAAAGTGGA GCAGTTCAGA CCATGGAGCC CCTCATCCAG 
GCAGCCCAGC TCCTCCAGCT GAAGAAGAAA ACCCACGAGG ATGCTGAGGC CATCTGCTCT 
CTGTGCACCT CCCTCAGCAC CCAGCAGATT GTCAAAATTT TAAACCTCTA CACTCCCTTG 
AATGAATTTG AGGAACGGGT CACAGTGTCC TTCATACGAA CAATCCAGGC TCAGCTACAA 
GAGAGGAATG ACCCTCAGCA GCTCCTGCTG GACTCCAAGC ACGTGTTCCC AGTTCTGTTT 
CCATATAACC CATCTGCTCT GACCATGGAC TCGATCCACA TCCCGGCCTG TCTCAACCTG 
GAGTTTCTCA ATGAAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Kinetic signatures of myosin-5B, the motor involved in microvillus inclusion disease.
The Journal of biological chemistry292(44)18372-18385(2017 11)
Heissler SM,Chinthalapudi K,Sellers JR

Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice.
Open biology7(1)(2017 Jan)
Liu TY,Chen YC,Jong YJ,Tsai HJ,Lee CC,Chang YS,Chang JG,Chang YF

Loss of MYO5B in mice recapitulates Microvillus Inclusion Disease and reveals an apical trafficking pathway distinct to neonatal duodenum.
Cellular and molecular gastroenterology and hepatology2(2)131-157(2016 02)
Weis VG,Knowles BC,Choi E,Goldstein AE,Williams JA,Manning EH,Roland JT,Lapierre LA,Goldenring JR

An inducible mouse model for microvillus inclusion disease reveals a role for myosin Vb in apical and basolateral trafficking.
Proceedings of the National Academy of Sciences of the United States of America112(40)12408-13(2015 Oct)
Schneeberger K,Vogel GF,Teunissen H,van Ommen DD,Begthel H,El Bouazzaoui L,van Vugt AH,Beekman JM,Klumperman J,M?ller T,Janecke A,Gerner P,Huber LA,Hess MW,Clevers H,van Es JH,Nieuwenhuis EE,Middendorp S

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Myo5b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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