MYO5B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYO5B
Entrez Gene ID	4645
Full Name	myosin VB
General protein information	Preferred Names myosin VB Names unconventional myosin-Vb MYO5B variant protein myosin-Vb
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18q21.1
Summary	The protein encoded by this gene, together with other proteins, may be involved in plasma membrane recycling. Mutations in this gene are associated with microvillous inclusion disease. [provided by RefSeq, Sep 2009].
Disorder MIM:	606540
Disorder Html:	Microvillus inclusion disease, 251850 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001080467.2	NP_001073936.1	unconventional myosin-Vb
NM_001080467.3	NP_001073936.1	unconventional myosin-Vb

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	myo5b	XP_002936554.2
Pan troglodytes (chimpanzee)	MYO5B	XP_003953333.1
Oryza sativa (rice)	Os02g0777700	NP_001048291.2
	MYO5B	XP_001090434.1
Canis lupus familiaris (dog)	MYO5B	XP_005623182.1
Bos taurus (cattle)	MYO5B	XP_002697867.2
Mus musculus (house mouse)	Myo5b	NP_963894.1
Danio rerio (zebrafish)	myo5b	NP_001155104.1
Schizosaccharomyces pombe (fission yeast)	myo52	NP_588492.1
Arabidopsis thaliana (thale cress)	XI-I	NP_195046.3
Homo sapiens (human)	MYO5B	NP_001073936.1
Rattus norvegicus (Norway rat)	Myo5b	NP_058779.1
Danio rerio (zebrafish)	LOC100536519	XP_003200915.2

Gene Ontology
Bibliography

Related articles in PubMed

Human Rab small GTPase- and class V myosin-mediated membrane tethering in a chemically defined reconstitution system.
Inoshita M, Mima J
The Journal of biological chemistry292(45)18500-18517(2017 11)

Kinetic signatures of myosin-5B, the motor involved in microvillus inclusion disease.
Heissler SM, Chinthalapudi K, Sellers JR
The Journal of biological chemistry292(44)18372-18385(2017 11)

Defects in myosin VB are associated with a spectrum of previously undiagnosed low ?-glutamyltransferase cholestasis.
Qiu YL, Gong JY, Feng JY, Wang RX, Han J, Liu T, Lu Y, Li LT, Zhang MH, Sheps JA, Wang NL, Yan YY, Li JQ, Chen L, Borchers CH, Sipos B, Knisely AS, Ling V, Xing QH, Wang JS
Hepatology (Baltimore, Md.)65(5)1655-1669(2017 05)

MYO5B mutations cause cholestasis with normal serum gamma-glutamyl transferase activity in children without microvillous inclusion disease.
Gonzales E, Taylor SA, Davit-Spraul A, Th?baut A, Thomassin N, Guettier C, Whitington PF, Jacquemin E
Hepatology (Baltimore, Md.)65(1)164-173(2017 01)

Myosin Vb mediates Cu+ export in polarized hepatocytes.
Gupta A, Schell MJ, Bhattacharjee A, Lutsenko S, Hubbard AL
Journal of cell science129(6)1179-89(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that membrane tethering mediated by endosomal RAB11A is drastically and selectively stimulated by its cognate Rab effectors, class V myosins (MYO5A and MYO5B), in a GTP-dependent manner. (RAB11A = ras-related GTPase Rab-11A; MYO5 = myosin class V)
Title: Human Rab small GTPase- and class V myosin-mediated membrane tethering in a chemically defined reconstitution system.

Data suggest that single-headed myosin-5B is intermediate-duty ratio motor with kinetic ATPase cycle, rate-limited by phosphate release; in transition to high-duty ratio motor, second head generates strain/gating in myosin-5B dimer that alter kinetic signature by slowing actin-activated ADP release to become rate-limiting; myosin-5B mutations cause microvillus inclusion disease and impact myosin motor function.
Title: Kinetic signatures of myosin-5B, the motor involved in microvillus inclusion disease.

MYO5B deficiency may underlie 20% of previously undiagnosed low-gamma-glutamyltransferase cholestasis. MYO5B deficiency appears to impair targeting of bile salt export pump to the canalicular membrane with hampered bile acid excretion, resulting in a spectrum of cholestasis without diarrhea.
Title: Defects in myosin VB are associated with a spectrum of previously undiagnosed low ?-glutamyltransferase cholestasis.

findings show for the first time that MYO5B deficiency may lead to cholestasis without microvillus inclusion disease
Title: MYO5B mutations cause cholestasis with normal serum gamma-glutamyl transferase activity in children without microvillous inclusion disease.

Myosin Vb regulates the Cu(+)-stimulated delivery of ATP7B to the apical domain of polarized hepatic cells.
Title: Myosin Vb mediates Cu+ export in polarized hepatocytes.

The following MYO5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYO5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18485	NM_001080467.3 Latest version!	Homo sapiens myosin VB (MYO5B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
View Old Accession Versions >>
OHu18485	NM_001080467.2	Homo sapiens myosin VB (MYO5B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYO5B ( NM_001080467.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001073936.1 Homo sapiens myosin VB (MYO5B), mRNA.

Price & Availability↑

CloneID	OHu18485
Clone ID Related Accession (Same CDS sequence)	NM_001080467.2 , NM_001080467.3
Accession Version	NM_001080467.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	unconventional myosin-Vb
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC032511.2, AY274809.1, AB290160.1, AK025336.1, BX641287.1, DA350416.1 and Z38208.1. This sequence is a reference standard in the RefSeqGene project. On Jun 13, 2009 this sequence version replaced NM_001080467.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB290160.1, SRR1803612.123286.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGTGG GCGAGCTCTA CAGCCAGTGC ACAAGGGTCT GGATCCCTGA CCCTGATGAG 
GTATGGCGCT CAGCTGAGTT AACCAAGGAC TACAAAGAAG GAGACAAGAG CCTACAGCTC 
AGACTGGAGG ATGAAACGAT TCTGGAATAC CCAATTGATG TACAACGCAA CCAGCTGCCC 
TTCTTACGGA ATCCAGATAT CTTGGTGGGA GAAAATGACC TGACTGCCCT TAGCTATCTT 
CATGAGCCTG CAGTTTTGCA TAATTTGAAG GTCCGTTTCC TGGAGTCCAA CCATATCTAC 
ACTTACTGTG GTATCGTACT TGTTGCCATT AATCCTTATG AACAGTTGCC AATCTATGGA 
CAAGATGTCA TCTATACCTA CAGTGGCCAA AACATGGGAG ACATGGACCC CCACATCTTT 
GCTGTGGCAG AAGAAGCCTA CAAGCAGATG GCCAGAGATG AGAAGAATCA GTCCATCATA 
GTCAGTGGGG AGTCTGGAGC CGGGAAGACG GTATCAGCCA AGTATGCCAT GCGCTATTTC 
GCCACCGTTG GTGGCTCGGC CAGTGAAACC AACATCGAAG AGAAGGTGCT GGCATCCAGT 
CCCATCATGG AGGCCATTGG AAATGCCAAG ACCACCCGCA ATGACAACAG CAGCCGTTTT 
GGCAAGTACA TCCAGATTGG CTTTGACAAA AGGTACCACA TCATCGGGGC CAACATGAGG 
ACTTACCTCT TGGAGAAGTC CAGAGTGGTC TTCCAGGCAG ATGATGAGAG GAATTACCAC 
ATCTTTTACC AGCTCTGTGC TGCTGCCGGT CTTCCAGAAT TTAAAGAGCT TGCACTAACA 
AGTGCAGAGG ACTTTTTCTA TACATCACAG GGAGGAGACA CTTCCATCGA GGGTGTGGAC 
GATGCTGAGG ACTTTGAGAA GACTCGACAA GCCTTCACAC TCCTCGGAGT GAAAGAGTCC 
CATCAGATGA GCATTTTTAA GATAATTGCT TCTATCTTGC ACCTTGGAAG TGTGGCGATT 
CAGGCTGAGC GTGATGGTGA TTCCTGTAGT ATATCACCCC AGGATGTATA CCTAAGCAAC 
TTCTGCCGAC TGCTAGGGGT GGAGCACAGT CAGATGGAGC ACTGGCTGTG TCATCGCAAG 
CTGGTCACCA CCTCGGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAGGT GATCAATGCG 
CGCAACGCCC TGGCGAAGCA CATCTATGCC CAGTTGTTCG GCTGGATTGT GGAGCACATC 
AACAAGGCCC TGCACACCTC CCTCAAGCAG CACTCCTTCA TCGGGGTCCT GGACATCTAT 
GGGTTTGAGA CATTTGAGGT AAACAGCTTT GAGCAGTTCT GTATCAACTA TGCAAATGAA 
AAGCTCCAGC AGCAGTTCAA CTCGCATGTT TTCAAACTGG AGCAAGAAGA ATACATGAAG 
GAACAGATCC CTTGGACCCT GATTGATTTT TATGATAACC AACCTTGTAT CGACCTCATT 
GAAGCCAAGC TGGGTATCTT GGACCTGTTG GATGAAGAAT GTAAGGTCCC CAAAGGAACT 
GACCAGAACT GGGCTCAGAA GCTCTATGAC CGGCACTCCA GCAGCCAGCA CTTCCAGAAG 
CCCCGCATGT CCAACACGGC CTTCATCATC GTCCACTTTG CAGACAAGGT GGAGTACCTC 
TCTGATGGTT TTCTGGAGAA AAACAGAGAC ACGGTGTATG AAGAGCAGAT CAATATCCTG 
AAGGCCAGCA AGTTCCCACT AGTGGCTGAC TTGTTTCATG ATGACAAGGA CCCTGTTCCT 
GCCACCACCC CTGGGAAGGG GTCATCTTCG AAGATCAGCG TCCGTTCTGC CAGACCCCCC 
ATGAAAGTCT CCAACAAGGA GCACAAGAAA ACCGTTGGCC ACCAGTTCCG TACCTCCCTG 
CATCTGCTCA TGGAGACCCT GAATGCCACG ACACCTCACT ATGTCCGCTG CATCAAGCCC 
AACGATGAGA AGCTCCCCTT TCACTTTGAC CCAAAGAGAG CAGTGCAGCA ACTCAGAGCC 
TGCGGGGTGT TGGAGACGAT TCGAATCAGT GCAGCTGGCT ACCCATCCAG GTGGGCCTAC 
CATGACTTTT TCAACCGGTA TCGGGTGCTG GTCAAGAAGA GAGAGCTCGC CAACACAGAC 
AAAAAGGCCA TCTGCAGGTC TGTCCTGGAG AACCTCATCA AGGACCCCGA CAAGTTCCAG 
TTTGGCCGCA CCAAGATCTT CTTTCGAGCA GGCCAGGTGG CCTACCTGGA GAAGCTGCGG 
GCTGACAAGT TCCGGACAGC CACCATCATG ATCCAGAAAA CTGTCCGGGG ATGGCTGCAG 
AAGGTGAAAT ATCACAGGCT GAAGGGGGCT ACCTTAACCC TGCAGAGGTA CTGCCGGGGA 
CACCTGGCCC GCAGGCTGGC TGAGCACCTG CGGAGGATCA GAGCGGCTGT GGTGCTCCAG 
AAACATTACC GCATGCAGAG GGCCCGCCAG GCCTACCAGA GGGTCCGCAG AGCTGCCGTT 
GTTATCCAGG CCTTCACCCG GGCCATGTTT GTGCGGAGAA CCTACCGCCA GGTCCTCATG 
GAGCACAAGG CCACCACCAT CCAGAAGCAC GTGCGGGGCT GGATGGCACG CAGGCACTTC 
CAGCGGCTGC GGGATGCAGC CATTGTCATC CAGTGTGCCT TCCGGATGCT CAAGGCCAGG 
CGGGAGCTGA AGGCCCTCAG GATTGAGGCC CGCTCAGCAG AGCATCTGAA ACGTCTCAAC 
GTGGGCATGG AGAACAAGGT GGTCCAGCTG CAGCGGAAGA TCGATGAGCA GAACAAAGAG 
TTCAAGACAC TTTCAGAGCA GTTGTCCGTG ACCACCTCAA CATACACCAT GGAGGTAGAG 
CGGCTGAAGA AGGAGCTGGT GCACTACCAG CAGAGCCCAG GTGAGGACAC CAGCCTCAGG 
CTGCAGGAGG AGGTGGAGAG CCTGCGCACA GAGCTGCAGA GGGCCCACTC GGAGCGCAAG 
ATCTTGGAGG ACGCCCACAG CAGGGAGAAA GATGAGCTGA GGAAGCGAGT TGCAGACCTG 
GAGCAAGAAA ATGCTCTCTT GAAAGATGAG AAAGAACAGC TCAACAACCA AATCCTGTGC 
CAGTCTAAAG ATGAATTTGC CCAGAACTCT GTGAAGGAAA ATCTCATGAA GAAAGAACTG 
GAGGAGGAGC GATCCCGGTA CCAGAACCTT GTGAAGGAAT ATTCACAGTT GGAGCAGAGA 
TACGACAACC TTCGGGATGA AATGACCATC ATAAAGCAAA CTCCAGGTCA TAGGCGGAAC 
CCATCAAACC AAAGTAGCTT AGAATCTGAC TCCAATTACC CCTCCATCTC CACATCTGAG 
ATCGGAGACA CTGAGGATGC CCTCCAGCAG GTGGAGGAAA TTGGCCTGGA GAAGGCAGCC 
ATGGACATGA CGGTCTTCCT GAAGCTGCAG AAGAGAGTAC GGGAGCTGGA GCAGGAGAGG 
AAAAAGCTGC AAGTGCAGCT GGAGAAGAGA GAACAGCAGG ACAGCAAGAA AGTCCAGGCG 
GAACCACCAC AGACTGACAT AGATTTGGAC CCGAATGCAG ATCTGGCCTA CAATAGTCTG 
AAGAGGCAAG AGCTGGAGTC AGAGAACAAA AAGCTGAAGA ATGACCTGAA TGAGCTGAGG 
AAAGCCGTGG CCGACCAAGC CACGCAGAAT AACTCCAGCC ACGGCTCCCC AGATAGCTAC 
AGCCTCCTGC TGAACCAGCT CAAGCTGGCC CACGAGGAGC TCGAGGTGCG CAAGGAGGAG 
GTGCTCATCC TCAGGACCCA GATCGTGAGC GCCGACCAGC GGCGACTCGC CGGCAGGAAC 
GCGGAGCCGA ACATTAATGC CAGATCAAGT TGGCCTAACA GTGAAAAGCA TGTTGACCAG 
GAGGATGCCA TTGAGGCCTA TCACGGGGTC TGCCAGACAA ACAGCAAGAC TGAGGATTGG 
GGATATTTAA ATGAAGATGG AGAACTCGGC TTGGCCTACC AAGGCCTAAA GCAAGTTGCC 
AGGCTGCTGG AGGCTCAGCT GCAGGCCCAG AGCCTGGAGC ATGAGGAGGA GGTGGAGCAT 
CTCAAGGCTC AGCTCGAGGC CCTGAAGGAG GAGATGGACA AACAGCAGCA GACCTTCTGC 
CAGACGCTAC TGCTCTCCCC AGAGGCCCAG GTGGAATTCG GCGTTCAGCA GGAAATATCC 
CGGCTGACCA ACGAGAATCT GGACCTTAAA GAACTGGTAG AAAAGCTGGA AAAGAATGAG 
AGGAAGCTCA AAAAGCAACT GAAGATTTAC ATGAAGAAAG CCCAGGACCT AGAAGCTGCC 
CAGGCATTGG CCCAGAGTGA GAGGAAGCGC CATGAGCTCA ACAGGCAGGT CACGGTCCAG 
CGGAAAGAGA AGGATTTCCA GGGCATGCTG GAGTACCACA AAGAGGACGA GGCCCTCCTC 
ATCCGGAACC TGGTGACAGA CTTGAAGCCC CAGATGCTGT CGGGCACAGT GCCCTGTCTC 
CCCGCCTACA TCCTCTACAT GTGCATCCGG CACGCGGACT ACACCAACGA CGATCTCAAG 
GTGCACTCCC TGCTGACCTC CACCATCAAC GGCATTAAGA AAGTCCTGAA AAAGCACAAT 
GATGACTTTG AGATGACGTC ATTCTGGTTA TCCAACACCT GCCGCCTTCT TCACTGTCTG 
AAGCAGTACA GCGGGGATGA GGGCTTCATG ACTCAGAACA CTGCAAAGCA GAATGAACAC 
TGTCTTAAGA ATTTTGACCT CACCGAATAC CGTCAGGTGC TGAGTGACCT TTCCATTCAG 
ATCTACCAGC AGCTCATTAA AATTGCCGAG GGCGTGTTAC AGCCGATGAT AGTTTCTGCC 
ATGTTGGAAA ATGAGAGCAT TCAGGGTCTA TCTGGTGTGA AGCCCACCGG CTACCGGAAG 
CGCTCCTCCA GCATGGCAGA TGGGGATAAC TCATACTGCC TGGAAGCTAT CATCCGCCAG 
ATGAATGCCT TTCATACAGT CATGTGTGAC CAGGGCTTGG ACCCTGAGAT CATCCTGCAG 
GTATTCAAAC AGCTCTTCTA CATGATCAAC GCAGTGACTC TTAACAACCT GCTCTTGCGG 
AAGGACGTCT GCTCTTGGAG CACAGGCATG CAACTCAGGT ACAATATAAG TCAGCTTGAG 
GAGTGGCTTC GGGGAAGAAA CCTTCACCAG AGTGGAGCAG TTCAGACCAT GGAACCTCTG 
ATCCAAGCAG CCCAGCTCCT GCAATTAAAG AAGAAAACCC AGGAGGACGC AGAGGCTATC 
TGCTCCCTGT GTACCTCCCT CAGCACCCAG CAGATTGTCA AAATTTTAAA CCTTTATACT 
CCCCTGAATG AATTTGAAGA ACGGGTAACA GTGGCCTTTA TACGAACAAT CCAGGCACAA 
CTACAAGAGC GGAATGACCC TCAGCAACTG CTATTAGATG CCAAGCACAT GTTTCCTGTT 
TTGTTTCCAT TTAATCCATC TTCTCTAACC ATGGACTCAA TCCACATCCC AGCGTGTCTC 
AATCTGGAAT TCCTCAATGA AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073936.1
CDS	289..5835
Translation	MSVGELYSQCTRVWIPDPDEVWRSAELTKDYKEGDKSLQLRLEDETILEYPIDVQRNQLPFLRNPDILVGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLPIYGQDVIYTYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASETNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYHIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAAGLPEFKELALTSAEDFFYTSQGGDTSIEGVDDAEDFEKTRQAFTLLGVKESHQMSIFKIIASILHLGSVAIQAERDGDSCSISPQDVYLSNFCRLLGVEHSQMEHWLCHRKLVTTSETYVKTMSLQQVINARNALAKHIYAQLFGWIVEHINKALHTSLKQHSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYDRHSSSQHFQKPRMSNTAFIIVHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDDKDPVPATTPGKGSSSKISVRSARPPMKVSNKEHKKTVGHQFRTSLHLLMETLNATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWAYHDFFNRYRVLVKKRELANTDKKAICRSVLENLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRTATIMIQKTVRGWLQKVKYHRLKGATLTLQRYCRGHLARRLAEHLRRIRAAVVLQKHYRMQRARQAYQRVRRAAVVIQAFTRAMFVRRTYRQVLMEHKATTIQKHVRGWMARRHFQRLRDAAIVIQCAFRMLKARRELKALRIEARSAEHLKRLNVGMENKVVQLQRKIDEQNKEFKTLSEQLSVTTSTYTMEVERLKKELVHYQQSPGEDTSLRLQEEVESLRTELQRAHSERKILEDAHSREKDELRKRVADLEQENALLKDEKEQLNNQILCQSKDEFAQNSVKENLMKKELEEERSRYQNLVKEYSQLEQRYDNLRDEMTIIKQTPGHRRNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGLEKAAMDMTVFLKLQKRVRELEQERKKLQVQLEKREQQDSKKVQAEPPQTDIDLDPNADLAYNSLKRQELESENKKLKNDLNELRKAVADQATQNNSSHGSPDSYSLLLNQLKLAHEELEVRKEEVLILRTQIVSADQRRLAGRNAEPNINARSSWPNSEKHVDQEDAIEAYHGVCQTNSKTEDWGYLNEDGELGLAYQGLKQVARLLEAQLQAQSLEHEEEVEHLKAQLEALKEEMDKQQQTFCQTLLLSPEAQVEFGVQQEISRLTNENLDLKELVEKLEKNERKLKKQLKIYMKKAQDLEAAQALAQSERKRHELNRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTDLKPQMLSGTVPCLPAYILYMCIRHADYTNDDLKVHSLLTSTINGIKKVLKKHNDDFEMTSFWLSNTCRLLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGVLQPMIVSAMLENESIQGLSGVKPTGYRKRSSSMADGDNSYCLEAIIRQMNAFHTVMCDQGLDPEIILQVFKQLFYMINAVTLNNLLLRKDVCSWSTGMQLRYNISQLEEWLRGRNLHQSGAVQTMEPLIQAAQLLQLKKKTQEDAEAICSLCTSLSTQQIVKILNLYTPLNEFEERVTVAFIRTIQAQLQERNDPQQLLLDAKHMFPVLFPFNPSSLTMDSIHIPACLNLEFLNEV

Target ORF information:

RefSeq Version	NM_001080467.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VB (MYO5B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080467.2

ORF Insert Sequence:

ATGTCGGTGG GCGAGCTCTA CAGCCAGTGC ACAAGGGTCT GGATCCCTGA CCCTGATGAG 
GTATGGCGCT CAGCTGAGTT AACCAAGGAC TACAAAGAAG GAGACAAGAG CCTACAGCTC 
AGACTGGAGG ATGAAACGAT TCTGGAATAC CCAATTGATG TACAACGCAA CCAGCTGCCC 
TTCTTACGGA ATCCAGATAT CTTGGTGGGA GAAAATGACC TGACTGCCCT TAGCTATCTT 
CATGAGCCTG CAGTTTTGCA TAATTTGAAG GTCCGTTTCC TGGAGTCCAA CCATATCTAC 
ACTTACTGTG GTATCGTACT TGTTGCCATT AATCCTTATG AACAGTTGCC AATCTATGGA 
CAAGATGTCA TCTATACCTA CAGTGGCCAA AACATGGGAG ACATGGACCC CCACATCTTT 
GCTGTGGCAG AAGAAGCCTA CAAGCAGATG GCCAGAGATG AGAAGAATCA GTCCATCATA 
GTCAGTGGGG AGTCTGGAGC CGGGAAGACG GTATCAGCCA AGTATGCCAT GCGCTATTTC 
GCCACCGTTG GTGGCTCGGC CAGTGAAACC AACATCGAAG AGAAGGTGCT GGCATCCAGT 
CCCATCATGG AGGCCATTGG AAATGCCAAG ACCACCCGCA ATGACAACAG CAGCCGTTTT 
GGCAAGTACA TCCAGATTGG CTTTGACAAA AGGTACCACA TCATCGGGGC CAACATGAGG 
ACTTACCTCT TGGAGAAGTC CAGAGTGGTC TTCCAGGCAG ATGATGAGAG GAATTACCAC 
ATCTTTTACC AGCTCTGTGC TGCTGCCGGT CTTCCAGAAT TTAAAGAGCT TGCACTAACA 
AGTGCAGAGG ACTTTTTCTA TACATCACAG GGAGGAGACA CTTCCATCGA GGGTGTGGAC 
GATGCTGAGG ACTTTGAGAA GACTCGACAA GCCTTCACAC TCCTCGGAGT GAAAGAGTCC 
CATCAGATGA GCATTTTTAA GATAATTGCT TCTATCTTGC ACCTTGGAAG TGTGGCGATT 
CAGGCTGAGC GTGATGGTGA TTCCTGTAGT ATATCACCCC AGGATGTATA CCTAAGCAAC 
TTCTGCCGAC TGCTAGGGGT GGAGCACAGT CAGATGGAGC ACTGGCTGTG TCATCGCAAG 
CTGGTCACCA CCTCGGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAGGT GATCAATGCG 
CGCAACGCCC TGGCGAAGCA CATCTATGCC CAGTTGTTCG GCTGGATTGT GGAGCACATC 
AACAAGGCCC TGCACACCTC CCTCAAGCAG CACTCCTTCA TCGGGGTCCT GGACATCTAT 
GGGTTTGAGA CATTTGAGGT AAACAGCTTT GAGCAGTTCT GTATCAACTA TGCAAATGAA 
AAGCTCCAGC AGCAGTTCAA CTCGCATGTT TTCAAACTGG AGCAAGAAGA ATACATGAAG 
GAACAGATCC CTTGGACCCT GATTGATTTT TATGATAACC AACCTTGTAT CGACCTCATT 
GAAGCCAAGC TGGGTATCTT GGACCTGTTG GATGAAGAAT GTAAGGTCCC CAAAGGAACT 
GACCAGAACT GGGCTCAGAA GCTCTATGAC CGGCACTCCA GCAGCCAGCA CTTCCAGAAG 
CCCCGCATGT CCAACACGGC CTTCATCATC GTCCACTTTG CAGACAAGGT GGAGTACCTC 
TCTGATGGTT TTCTGGAGAA AAACAGAGAC ACGGTGTATG AAGAGCAGAT CAATATCCTG 
AAGGCCAGCA AGTTCCCACT AGTGGCTGAC TTGTTTCATG ATGACAAGGA CCCTGTTCCT 
GCCACCACCC CTGGGAAGGG GTCATCTTCG AAGATCAGCG TCCGTTCTGC CAGACCCCCC 
ATGAAAGTCT CCAACAAGGA GCACAAGAAA ACCGTTGGCC ACCAGTTCCG TACCTCCCTG 
CATCTGCTCA TGGAGACCCT GAATGCCACG ACACCTCACT ATGTCCGCTG CATCAAGCCC 
AACGATGAGA AGCTCCCCTT TCACTTTGAC CCAAAGAGAG CAGTGCAGCA ACTCAGAGCC 
TGCGGGGTGT TGGAGACGAT TCGAATCAGT GCAGCTGGCT ACCCATCCAG GTGGGCCTAC 
CATGACTTTT TCAACCGGTA TCGGGTGCTG GTCAAGAAGA GAGAGCTCGC CAACACAGAC 
AAAAAGGCCA TCTGCAGGTC TGTCCTGGAG AACCTCATCA AGGACCCCGA CAAGTTCCAG 
TTTGGCCGCA CCAAGATCTT CTTTCGAGCA GGCCAGGTGG CCTACCTGGA GAAGCTGCGG 
GCTGACAAGT TCCGGACAGC CACCATCATG ATCCAGAAAA CTGTCCGGGG ATGGCTGCAG 
AAGGTGAAAT ATCACAGGCT GAAGGGGGCT ACCTTAACCC TGCAGAGGTA CTGCCGGGGA 
CACCTGGCCC GCAGGCTGGC TGAGCACCTG CGGAGGATCA GAGCGGCTGT GGTGCTCCAG 
AAACATTACC GCATGCAGAG GGCCCGCCAG GCCTACCAGA GGGTCCGCAG AGCTGCCGTT 
GTTATCCAGG CCTTCACCCG GGCCATGTTT GTGCGGAGAA CCTACCGCCA GGTCCTCATG 
GAGCACAAGG CCACCACCAT CCAGAAGCAC GTGCGGGGCT GGATGGCACG CAGGCACTTC 
CAGCGGCTGC GGGATGCAGC CATTGTCATC CAGTGTGCCT TCCGGATGCT CAAGGCCAGG 
CGGGAGCTGA AGGCCCTCAG GATTGAGGCC CGCTCAGCAG AGCATCTGAA ACGTCTCAAC 
GTGGGCATGG AGAACAAGGT GGTCCAGCTG CAGCGGAAGA TCGATGAGCA GAACAAAGAG 
TTCAAGACAC TTTCAGAGCA GTTGTCCGTG ACCACCTCAA CATACACCAT GGAGGTAGAG 
CGGCTGAAGA AGGAGCTGGT GCACTACCAG CAGAGCCCAG GTGAGGACAC CAGCCTCAGG 
CTGCAGGAGG AGGTGGAGAG CCTGCGCACA GAGCTGCAGA GGGCCCACTC GGAGCGCAAG 
ATCTTGGAGG ACGCCCACAG CAGGGAGAAA GATGAGCTGA GGAAGCGAGT TGCAGACCTG 
GAGCAAGAAA ATGCTCTCTT GAAAGATGAG AAAGAACAGC TCAACAACCA AATCCTGTGC 
CAGTCTAAAG ATGAATTTGC CCAGAACTCT GTGAAGGAAA ATCTCATGAA GAAAGAACTG 
GAGGAGGAGC GATCCCGGTA CCAGAACCTT GTGAAGGAAT ATTCACAGTT GGAGCAGAGA 
TACGACAACC TTCGGGATGA AATGACCATC ATAAAGCAAA CTCCAGGTCA TAGGCGGAAC 
CCATCAAACC AAAGTAGCTT AGAATCTGAC TCCAATTACC CCTCCATCTC CACATCTGAG 
ATCGGAGACA CTGAGGATGC CCTCCAGCAG GTGGAGGAAA TTGGCCTGGA GAAGGCAGCC 
ATGGACATGA CGGTCTTCCT GAAGCTGCAG AAGAGAGTAC GGGAGCTGGA GCAGGAGAGG 
AAAAAGCTGC AAGTGCAGCT GGAGAAGAGA GAACAGCAGG ACAGCAAGAA AGTCCAGGCG 
GAACCACCAC AGACTGACAT AGATTTGGAC CCGAATGCAG ATCTGGCCTA CAATAGTCTG 
AAGAGGCAAG AGCTGGAGTC AGAGAACAAA AAGCTGAAGA ATGACCTGAA TGAGCTGAGG 
AAAGCCGTGG CCGACCAAGC CACGCAGAAT AACTCCAGCC ACGGCTCCCC AGATAGCTAC 
AGCCTCCTGC TGAACCAGCT CAAGCTGGCC CACGAGGAGC TCGAGGTGCG CAAGGAGGAG 
GTGCTCATCC TCAGGACCCA GATCGTGAGC GCCGACCAGC GGCGACTCGC CGGCAGGAAC 
GCGGAGCCGA ACATTAATGC CAGATCAAGT TGGCCTAACA GTGAAAAGCA TGTTGACCAG 
GAGGATGCCA TTGAGGCCTA TCACGGGGTC TGCCAGACAA ACAGCAAGAC TGAGGATTGG 
GGATATTTAA ATGAAGATGG AGAACTCGGC TTGGCCTACC AAGGCCTAAA GCAAGTTGCC 
AGGCTGCTGG AGGCTCAGCT GCAGGCCCAG AGCCTGGAGC ATGAGGAGGA GGTGGAGCAT 
CTCAAGGCTC AGCTCGAGGC CCTGAAGGAG GAGATGGACA AACAGCAGCA GACCTTCTGC 
CAGACGCTAC TGCTCTCCCC AGAGGCCCAG GTGGAATTCG GCGTTCAGCA GGAAATATCC 
CGGCTGACCA ACGAGAATCT GGACCTTAAA GAACTGGTAG AAAAGCTGGA AAAGAATGAG 
AGGAAGCTCA AAAAGCAACT GAAGATTTAC ATGAAGAAAG CCCAGGACCT AGAAGCTGCC 
CAGGCATTGG CCCAGAGTGA GAGGAAGCGC CATGAGCTCA ACAGGCAGGT CACGGTCCAG 
CGGAAAGAGA AGGATTTCCA GGGCATGCTG GAGTACCACA AAGAGGACGA GGCCCTCCTC 
ATCCGGAACC TGGTGACAGA CTTGAAGCCC CAGATGCTGT CGGGCACAGT GCCCTGTCTC 
CCCGCCTACA TCCTCTACAT GTGCATCCGG CACGCGGACT ACACCAACGA CGATCTCAAG 
GTGCACTCCC TGCTGACCTC CACCATCAAC GGCATTAAGA AAGTCCTGAA AAAGCACAAT 
GATGACTTTG AGATGACGTC ATTCTGGTTA TCCAACACCT GCCGCCTTCT TCACTGTCTG 
AAGCAGTACA GCGGGGATGA GGGCTTCATG ACTCAGAACA CTGCAAAGCA GAATGAACAC 
TGTCTTAAGA ATTTTGACCT CACCGAATAC CGTCAGGTGC TGAGTGACCT TTCCATTCAG 
ATCTACCAGC AGCTCATTAA AATTGCCGAG GGCGTGTTAC AGCCGATGAT AGTTTCTGCC 
ATGTTGGAAA ATGAGAGCAT TCAGGGTCTA TCTGGTGTGA AGCCCACCGG CTACCGGAAG 
CGCTCCTCCA GCATGGCAGA TGGGGATAAC TCATACTGCC TGGAAGCTAT CATCCGCCAG 
ATGAATGCCT TTCATACAGT CATGTGTGAC CAGGGCTTGG ACCCTGAGAT CATCCTGCAG 
GTATTCAAAC AGCTCTTCTA CATGATCAAC GCAGTGACTC TTAACAACCT GCTCTTGCGG 
AAGGACGTCT GCTCTTGGAG CACAGGCATG CAACTCAGGT ACAATATAAG TCAGCTTGAG 
GAGTGGCTTC GGGGAAGAAA CCTTCACCAG AGTGGAGCAG TTCAGACCAT GGAACCTCTG 
ATCCAAGCAG CCCAGCTCCT GCAATTAAAG AAGAAAACCC AGGAGGACGC AGAGGCTATC 
TGCTCCCTGT GTACCTCCCT CAGCACCCAG CAGATTGTCA AAATTTTAAA CCTTTATACT 
CCCCTGAATG AATTTGAAGA ACGGGTAACA GTGGCCTTTA TACGAACAAT CCAGGCACAA 
CTACAAGAGC GGAATGACCC TCAGCAACTG CTATTAGATG CCAAGCACAT GTTTCCTGTT 
TTGTTTCCAT TTAATCCATC TTCTCTAACC ATGGACTCAA TCCACATCCC AGCGTGTCTC 
AATCTGGAAT TCCTCAATGA AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5B ( NM_001080467.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001073936.1 Homo sapiens myosin VB (MYO5B), mRNA.

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CloneID	OHu18485
Clone ID Related Accession (Same CDS sequence)	NM_001080467.2 , NM_001080467.3
Accession Version	NM_001080467.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	unconventional myosin-Vb
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC105224.8, BC032511.2, AY274809.1, AB290160.1, AK025336.1, BX641287.1, DA350416.1 and Z38208.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001080467.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB290160.1, AB032945.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000285039.12/ ENSP00000285039.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGGTGG GCGAGCTCTA CAGCCAGTGC ACAAGGGTCT GGATCCCTGA CCCTGATGAG 
GTATGGCGCT CAGCTGAGTT AACCAAGGAC TACAAAGAAG GAGACAAGAG CCTACAGCTC 
AGACTGGAGG ATGAAACGAT TCTGGAATAC CCAATTGATG TACAACGCAA CCAGCTGCCC 
TTCTTACGGA ATCCAGATAT CTTGGTGGGA GAAAATGACC TGACTGCCCT TAGCTATCTT 
CATGAGCCTG CAGTTTTGCA TAATTTGAAG GTCCGTTTCC TGGAGTCCAA CCATATCTAC 
ACTTACTGTG GTATCGTACT TGTTGCCATT AATCCTTATG AACAGTTGCC AATCTATGGA 
CAAGATGTCA TCTATACCTA CAGTGGCCAA AACATGGGAG ACATGGACCC CCACATCTTT 
GCTGTGGCAG AAGAAGCCTA CAAGCAGATG GCCAGAGATG AGAAGAATCA GTCCATCATA 
GTCAGTGGGG AGTCTGGAGC CGGGAAGACG GTATCAGCCA AGTATGCCAT GCGCTATTTC 
GCCACCGTTG GTGGCTCGGC CAGTGAAACC AACATCGAAG AGAAGGTGCT GGCATCCAGT 
CCCATCATGG AGGCCATTGG AAATGCCAAG ACCACCCGCA ATGACAACAG CAGCCGTTTT 
GGCAAGTACA TCCAGATTGG CTTTGACAAA AGGTACCACA TCATCGGGGC CAACATGAGG 
ACTTACCTCT TGGAGAAGTC CAGAGTGGTC TTCCAGGCAG ATGATGAGAG GAATTACCAC 
ATCTTTTACC AGCTCTGTGC TGCTGCCGGT CTTCCAGAAT TTAAAGAGCT TGCACTAACA 
AGTGCAGAGG ACTTTTTCTA TACATCACAG GGAGGAGACA CTTCCATCGA GGGTGTGGAC 
GATGCTGAGG ACTTTGAGAA GACTCGACAA GCCTTCACAC TCCTCGGAGT GAAAGAGTCC 
CATCAGATGA GCATTTTTAA GATAATTGCT TCTATCTTGC ACCTTGGAAG TGTGGCGATT 
CAGGCTGAGC GTGATGGTGA TTCCTGTAGT ATATCACCCC AGGATGTATA CCTAAGCAAC 
TTCTGCCGAC TGCTAGGGGT GGAGCACAGT CAGATGGAGC ACTGGCTGTG TCATCGCAAG 
CTGGTCACCA CCTCGGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAGGT GATCAATGCG 
CGCAACGCCC TGGCGAAGCA CATCTATGCC CAGTTGTTCG GCTGGATTGT GGAGCACATC 
AACAAGGCCC TGCACACCTC CCTCAAGCAG CACTCCTTCA TCGGGGTCCT GGACATCTAT 
GGGTTTGAGA CATTTGAGGT AAACAGCTTT GAGCAGTTCT GTATCAACTA TGCAAATGAA 
AAGCTCCAGC AGCAGTTCAA CTCGCATGTT TTCAAACTGG AGCAAGAAGA ATACATGAAG 
GAACAGATCC CTTGGACCCT GATTGATTTT TATGATAACC AACCTTGTAT CGACCTCATT 
GAAGCCAAGC TGGGTATCTT GGACCTGTTG GATGAAGAAT GTAAGGTCCC CAAAGGAACT 
GACCAGAACT GGGCTCAGAA GCTCTATGAC CGGCACTCCA GCAGCCAGCA CTTCCAGAAG 
CCCCGCATGT CCAACACGGC CTTCATCATC GTCCACTTTG CAGACAAGGT GGAGTACCTC 
TCTGATGGTT TTCTGGAGAA AAACAGAGAC ACGGTGTATG AAGAGCAGAT CAATATCCTG 
AAGGCCAGCA AGTTCCCACT AGTGGCTGAC TTGTTTCATG ATGACAAGGA CCCTGTTCCT 
GCCACCACCC CTGGGAAGGG GTCATCTTCG AAGATCAGCG TCCGTTCTGC CAGACCCCCC 
ATGAAAGTCT CCAACAAGGA GCACAAGAAA ACCGTTGGCC ACCAGTTCCG TACCTCCCTG 
CATCTGCTCA TGGAGACCCT GAATGCCACG ACACCTCACT ATGTCCGCTG CATCAAGCCC 
AACGATGAGA AGCTCCCCTT TCACTTTGAC CCAAAGAGAG CAGTGCAGCA ACTCAGAGCC 
TGCGGGGTGT TGGAGACGAT TCGAATCAGT GCAGCTGGCT ACCCATCCAG GTGGGCCTAC 
CATGACTTTT TCAACCGGTA TCGGGTGCTG GTCAAGAAGA GAGAGCTCGC CAACACAGAC 
AAAAAGGCCA TCTGCAGGTC TGTCCTGGAG AACCTCATCA AGGACCCCGA CAAGTTCCAG 
TTTGGCCGCA CCAAGATCTT CTTTCGAGCA GGCCAGGTGG CCTACCTGGA GAAGCTGCGG 
GCTGACAAGT TCCGGACAGC CACCATCATG ATCCAGAAAA CTGTCCGGGG ATGGCTGCAG 
AAGGTGAAAT ATCACAGGCT GAAGGGGGCT ACCTTAACCC TGCAGAGGTA CTGCCGGGGA 
CACCTGGCCC GCAGGCTGGC TGAGCACCTG CGGAGGATCA GAGCGGCTGT GGTGCTCCAG 
AAACATTACC GCATGCAGAG GGCCCGCCAG GCCTACCAGA GGGTCCGCAG AGCTGCCGTT 
GTTATCCAGG CCTTCACCCG GGCCATGTTT GTGCGGAGAA CCTACCGCCA GGTCCTCATG 
GAGCACAAGG CCACCACCAT CCAGAAGCAC GTGCGGGGCT GGATGGCACG CAGGCACTTC 
CAGCGGCTGC GGGATGCAGC CATTGTCATC CAGTGTGCCT TCCGGATGCT CAAGGCCAGG 
CGGGAGCTGA AGGCCCTCAG GATTGAGGCC CGCTCAGCAG AGCATCTGAA ACGTCTCAAC 
GTGGGCATGG AGAACAAGGT GGTCCAGCTG CAGCGGAAGA TCGATGAGCA GAACAAAGAG 
TTCAAGACAC TTTCAGAGCA GTTGTCCGTG ACCACCTCAA CATACACCAT GGAGGTAGAG 
CGGCTGAAGA AGGAGCTGGT GCACTACCAG CAGAGCCCAG GTGAGGACAC CAGCCTCAGG 
CTGCAGGAGG AGGTGGAGAG CCTGCGCACA GAGCTGCAGA GGGCCCACTC GGAGCGCAAG 
ATCTTGGAGG ACGCCCACAG CAGGGAGAAA GATGAGCTGA GGAAGCGAGT TGCAGACCTG 
GAGCAAGAAA ATGCTCTCTT GAAAGATGAG AAAGAACAGC TCAACAACCA AATCCTGTGC 
CAGTCTAAAG ATGAATTTGC CCAGAACTCT GTGAAGGAAA ATCTCATGAA GAAAGAACTG 
GAGGAGGAGC GATCCCGGTA CCAGAACCTT GTGAAGGAAT ATTCACAGTT GGAGCAGAGA 
TACGACAACC TTCGGGATGA AATGACCATC ATAAAGCAAA CTCCAGGTCA TAGGCGGAAC 
CCATCAAACC AAAGTAGCTT AGAATCTGAC TCCAATTACC CCTCCATCTC CACATCTGAG 
ATCGGAGACA CTGAGGATGC CCTCCAGCAG GTGGAGGAAA TTGGCCTGGA GAAGGCAGCC 
ATGGACATGA CGGTCTTCCT GAAGCTGCAG AAGAGAGTAC GGGAGCTGGA GCAGGAGAGG 
AAAAAGCTGC AAGTGCAGCT GGAGAAGAGA GAACAGCAGG ACAGCAAGAA AGTCCAGGCG 
GAACCACCAC AGACTGACAT AGATTTGGAC CCGAATGCAG ATCTGGCCTA CAATAGTCTG 
AAGAGGCAAG AGCTGGAGTC AGAGAACAAA AAGCTGAAGA ATGACCTGAA TGAGCTGAGG 
AAAGCCGTGG CCGACCAAGC CACGCAGAAT AACTCCAGCC ACGGCTCCCC AGATAGCTAC 
AGCCTCCTGC TGAACCAGCT CAAGCTGGCC CACGAGGAGC TCGAGGTGCG CAAGGAGGAG 
GTGCTCATCC TCAGGACCCA GATCGTGAGC GCCGACCAGC GGCGACTCGC CGGCAGGAAC 
GCGGAGCCGA ACATTAATGC CAGATCAAGT TGGCCTAACA GTGAAAAGCA TGTTGACCAG 
GAGGATGCCA TTGAGGCCTA TCACGGGGTC TGCCAGACAA ACAGCAAGAC TGAGGATTGG 
GGATATTTAA ATGAAGATGG AGAACTCGGC TTGGCCTACC AAGGCCTAAA GCAAGTTGCC 
AGGCTGCTGG AGGCTCAGCT GCAGGCCCAG AGCCTGGAGC ATGAGGAGGA GGTGGAGCAT 
CTCAAGGCTC AGCTCGAGGC CCTGAAGGAG GAGATGGACA AACAGCAGCA GACCTTCTGC 
CAGACGCTAC TGCTCTCCCC AGAGGCCCAG GTGGAATTCG GCGTTCAGCA GGAAATATCC 
CGGCTGACCA ACGAGAATCT GGACCTTAAA GAACTGGTAG AAAAGCTGGA AAAGAATGAG 
AGGAAGCTCA AAAAGCAACT GAAGATTTAC ATGAAGAAAG CCCAGGACCT AGAAGCTGCC 
CAGGCATTGG CCCAGAGTGA GAGGAAGCGC CATGAGCTCA ACAGGCAGGT CACGGTCCAG 
CGGAAAGAGA AGGATTTCCA GGGCATGCTG GAGTACCACA AAGAGGACGA GGCCCTCCTC 
ATCCGGAACC TGGTGACAGA CTTGAAGCCC CAGATGCTGT CGGGCACAGT GCCCTGTCTC 
CCCGCCTACA TCCTCTACAT GTGCATCCGG CACGCGGACT ACACCAACGA CGATCTCAAG 
GTGCACTCCC TGCTGACCTC CACCATCAAC GGCATTAAGA AAGTCCTGAA AAAGCACAAT 
GATGACTTTG AGATGACGTC ATTCTGGTTA TCCAACACCT GCCGCCTTCT TCACTGTCTG 
AAGCAGTACA GCGGGGATGA GGGCTTCATG ACTCAGAACA CTGCAAAGCA GAATGAACAC 
TGTCTTAAGA ATTTTGACCT CACCGAATAC CGTCAGGTGC TGAGTGACCT TTCCATTCAG 
ATCTACCAGC AGCTCATTAA AATTGCCGAG GGCGTGTTAC AGCCGATGAT AGTTTCTGCC 
ATGTTGGAAA ATGAGAGCAT TCAGGGTCTA TCTGGTGTGA AGCCCACCGG CTACCGGAAG 
CGCTCCTCCA GCATGGCAGA TGGGGATAAC TCATACTGCC TGGAAGCTAT CATCCGCCAG 
ATGAATGCCT TTCATACAGT CATGTGTGAC CAGGGCTTGG ACCCTGAGAT CATCCTGCAG 
GTATTCAAAC AGCTCTTCTA CATGATCAAC GCAGTGACTC TTAACAACCT GCTCTTGCGG 
AAGGACGTCT GCTCTTGGAG CACAGGCATG CAACTCAGGT ACAATATAAG TCAGCTTGAG 
GAGTGGCTTC GGGGAAGAAA CCTTCACCAG AGTGGAGCAG TTCAGACCAT GGAACCTCTG 
ATCCAAGCAG CCCAGCTCCT GCAATTAAAG AAGAAAACCC AGGAGGACGC AGAGGCTATC 
TGCTCCCTGT GTACCTCCCT CAGCACCCAG CAGATTGTCA AAATTTTAAA CCTTTATACT 
CCCCTGAATG AATTTGAAGA ACGGGTAACA GTGGCCTTTA TACGAACAAT CCAGGCACAA 
CTACAAGAGC GGAATGACCC TCAGCAACTG CTATTAGATG CCAAGCACAT GTTTCCTGTT 
TTGTTTCCAT TTAATCCATC TTCTCTAACC ATGGACTCAA TCCACATCCC AGCGTGTCTC 
AATCTGGAAT TCCTCAATGA AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073936.1
CDS	355..5901
Translation	MSVGELYSQCTRVWIPDPDEVWRSAELTKDYKEGDKSLQLRLEDETILEYPIDVQRNQLPFLRNPDILVGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLPIYGQDVIYTYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASETNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYHIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAAGLPEFKELALTSAEDFFYTSQGGDTSIEGVDDAEDFEKTRQAFTLLGVKESHQMSIFKIIASILHLGSVAIQAERDGDSCSISPQDVYLSNFCRLLGVEHSQMEHWLCHRKLVTTSETYVKTMSLQQVINARNALAKHIYAQLFGWIVEHINKALHTSLKQHSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYDRHSSSQHFQKPRMSNTAFIIVHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDDKDPVPATTPGKGSSSKISVRSARPPMKVSNKEHKKTVGHQFRTSLHLLMETLNATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWAYHDFFNRYRVLVKKRELANTDKKAICRSVLENLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRTATIMIQKTVRGWLQKVKYHRLKGATLTLQRYCRGHLARRLAEHLRRIRAAVVLQKHYRMQRARQAYQRVRRAAVVIQAFTRAMFVRRTYRQVLMEHKATTIQKHVRGWMARRHFQRLRDAAIVIQCAFRMLKARRELKALRIEARSAEHLKRLNVGMENKVVQLQRKIDEQNKEFKTLSEQLSVTTSTYTMEVERLKKELVHYQQSPGEDTSLRLQEEVESLRTELQRAHSERKILEDAHSREKDELRKRVADLEQENALLKDEKEQLNNQILCQSKDEFAQNSVKENLMKKELEEERSRYQNLVKEYSQLEQRYDNLRDEMTIIKQTPGHRRNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGLEKAAMDMTVFLKLQKRVRELEQERKKLQVQLEKREQQDSKKVQAEPPQTDIDLDPNADLAYNSLKRQELESENKKLKNDLNELRKAVADQATQNNSSHGSPDSYSLLLNQLKLAHEELEVRKEEVLILRTQIVSADQRRLAGRNAEPNINARSSWPNSEKHVDQEDAIEAYHGVCQTNSKTEDWGYLNEDGELGLAYQGLKQVARLLEAQLQAQSLEHEEEVEHLKAQLEALKEEMDKQQQTFCQTLLLSPEAQVEFGVQQEISRLTNENLDLKELVEKLEKNERKLKKQLKIYMKKAQDLEAAQALAQSERKRHELNRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTDLKPQMLSGTVPCLPAYILYMCIRHADYTNDDLKVHSLLTSTINGIKKVLKKHNDDFEMTSFWLSNTCRLLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGVLQPMIVSAMLENESIQGLSGVKPTGYRKRSSSMADGDNSYCLEAIIRQMNAFHTVMCDQGLDPEIILQVFKQLFYMINAVTLNNLLLRKDVCSWSTGMQLRYNISQLEEWLRGRNLHQSGAVQTMEPLIQAAQLLQLKKKTQEDAEAICSLCTSLSTQQIVKILNLYTPLNEFEERVTVAFIRTIQAQLQERNDPQQLLLDAKHMFPVLFPFNPSSLTMDSIHIPACLNLEFLNEV

Target ORF information:

RefSeq Version	NM_001080467.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VB (MYO5B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080467.3

ORF Insert Sequence:

ATGTCGGTGG GCGAGCTCTA CAGCCAGTGC ACAAGGGTCT GGATCCCTGA CCCTGATGAG 
GTATGGCGCT CAGCTGAGTT AACCAAGGAC TACAAAGAAG GAGACAAGAG CCTACAGCTC 
AGACTGGAGG ATGAAACGAT TCTGGAATAC CCAATTGATG TACAACGCAA CCAGCTGCCC 
TTCTTACGGA ATCCAGATAT CTTGGTGGGA GAAAATGACC TGACTGCCCT TAGCTATCTT 
CATGAGCCTG CAGTTTTGCA TAATTTGAAG GTCCGTTTCC TGGAGTCCAA CCATATCTAC 
ACTTACTGTG GTATCGTACT TGTTGCCATT AATCCTTATG AACAGTTGCC AATCTATGGA 
CAAGATGTCA TCTATACCTA CAGTGGCCAA AACATGGGAG ACATGGACCC CCACATCTTT 
GCTGTGGCAG AAGAAGCCTA CAAGCAGATG GCCAGAGATG AGAAGAATCA GTCCATCATA 
GTCAGTGGGG AGTCTGGAGC CGGGAAGACG GTATCAGCCA AGTATGCCAT GCGCTATTTC 
GCCACCGTTG GTGGCTCGGC CAGTGAAACC AACATCGAAG AGAAGGTGCT GGCATCCAGT 
CCCATCATGG AGGCCATTGG AAATGCCAAG ACCACCCGCA ATGACAACAG CAGCCGTTTT 
GGCAAGTACA TCCAGATTGG CTTTGACAAA AGGTACCACA TCATCGGGGC CAACATGAGG 
ACTTACCTCT TGGAGAAGTC CAGAGTGGTC TTCCAGGCAG ATGATGAGAG GAATTACCAC 
ATCTTTTACC AGCTCTGTGC TGCTGCCGGT CTTCCAGAAT TTAAAGAGCT TGCACTAACA 
AGTGCAGAGG ACTTTTTCTA TACATCACAG GGAGGAGACA CTTCCATCGA GGGTGTGGAC 
GATGCTGAGG ACTTTGAGAA GACTCGACAA GCCTTCACAC TCCTCGGAGT GAAAGAGTCC 
CATCAGATGA GCATTTTTAA GATAATTGCT TCTATCTTGC ACCTTGGAAG TGTGGCGATT 
CAGGCTGAGC GTGATGGTGA TTCCTGTAGT ATATCACCCC AGGATGTATA CCTAAGCAAC 
TTCTGCCGAC TGCTAGGGGT GGAGCACAGT CAGATGGAGC ACTGGCTGTG TCATCGCAAG 
CTGGTCACCA CCTCGGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAGGT GATCAATGCG 
CGCAACGCCC TGGCGAAGCA CATCTATGCC CAGTTGTTCG GCTGGATTGT GGAGCACATC 
AACAAGGCCC TGCACACCTC CCTCAAGCAG CACTCCTTCA TCGGGGTCCT GGACATCTAT 
GGGTTTGAGA CATTTGAGGT AAACAGCTTT GAGCAGTTCT GTATCAACTA TGCAAATGAA 
AAGCTCCAGC AGCAGTTCAA CTCGCATGTT TTCAAACTGG AGCAAGAAGA ATACATGAAG 
GAACAGATCC CTTGGACCCT GATTGATTTT TATGATAACC AACCTTGTAT CGACCTCATT 
GAAGCCAAGC TGGGTATCTT GGACCTGTTG GATGAAGAAT GTAAGGTCCC CAAAGGAACT 
GACCAGAACT GGGCTCAGAA GCTCTATGAC CGGCACTCCA GCAGCCAGCA CTTCCAGAAG 
CCCCGCATGT CCAACACGGC CTTCATCATC GTCCACTTTG CAGACAAGGT GGAGTACCTC 
TCTGATGGTT TTCTGGAGAA AAACAGAGAC ACGGTGTATG AAGAGCAGAT CAATATCCTG 
AAGGCCAGCA AGTTCCCACT AGTGGCTGAC TTGTTTCATG ATGACAAGGA CCCTGTTCCT 
GCCACCACCC CTGGGAAGGG GTCATCTTCG AAGATCAGCG TCCGTTCTGC CAGACCCCCC 
ATGAAAGTCT CCAACAAGGA GCACAAGAAA ACCGTTGGCC ACCAGTTCCG TACCTCCCTG 
CATCTGCTCA TGGAGACCCT GAATGCCACG ACACCTCACT ATGTCCGCTG CATCAAGCCC 
AACGATGAGA AGCTCCCCTT TCACTTTGAC CCAAAGAGAG CAGTGCAGCA ACTCAGAGCC 
TGCGGGGTGT TGGAGACGAT TCGAATCAGT GCAGCTGGCT ACCCATCCAG GTGGGCCTAC 
CATGACTTTT TCAACCGGTA TCGGGTGCTG GTCAAGAAGA GAGAGCTCGC CAACACAGAC 
AAAAAGGCCA TCTGCAGGTC TGTCCTGGAG AACCTCATCA AGGACCCCGA CAAGTTCCAG 
TTTGGCCGCA CCAAGATCTT CTTTCGAGCA GGCCAGGTGG CCTACCTGGA GAAGCTGCGG 
GCTGACAAGT TCCGGACAGC CACCATCATG ATCCAGAAAA CTGTCCGGGG ATGGCTGCAG 
AAGGTGAAAT ATCACAGGCT GAAGGGGGCT ACCTTAACCC TGCAGAGGTA CTGCCGGGGA 
CACCTGGCCC GCAGGCTGGC TGAGCACCTG CGGAGGATCA GAGCGGCTGT GGTGCTCCAG 
AAACATTACC GCATGCAGAG GGCCCGCCAG GCCTACCAGA GGGTCCGCAG AGCTGCCGTT 
GTTATCCAGG CCTTCACCCG GGCCATGTTT GTGCGGAGAA CCTACCGCCA GGTCCTCATG 
GAGCACAAGG CCACCACCAT CCAGAAGCAC GTGCGGGGCT GGATGGCACG CAGGCACTTC 
CAGCGGCTGC GGGATGCAGC CATTGTCATC CAGTGTGCCT TCCGGATGCT CAAGGCCAGG 
CGGGAGCTGA AGGCCCTCAG GATTGAGGCC CGCTCAGCAG AGCATCTGAA ACGTCTCAAC 
GTGGGCATGG AGAACAAGGT GGTCCAGCTG CAGCGGAAGA TCGATGAGCA GAACAAAGAG 
TTCAAGACAC TTTCAGAGCA GTTGTCCGTG ACCACCTCAA CATACACCAT GGAGGTAGAG 
CGGCTGAAGA AGGAGCTGGT GCACTACCAG CAGAGCCCAG GTGAGGACAC CAGCCTCAGG 
CTGCAGGAGG AGGTGGAGAG CCTGCGCACA GAGCTGCAGA GGGCCCACTC GGAGCGCAAG 
ATCTTGGAGG ACGCCCACAG CAGGGAGAAA GATGAGCTGA GGAAGCGAGT TGCAGACCTG 
GAGCAAGAAA ATGCTCTCTT GAAAGATGAG AAAGAACAGC TCAACAACCA AATCCTGTGC 
CAGTCTAAAG ATGAATTTGC CCAGAACTCT GTGAAGGAAA ATCTCATGAA GAAAGAACTG 
GAGGAGGAGC GATCCCGGTA CCAGAACCTT GTGAAGGAAT ATTCACAGTT GGAGCAGAGA 
TACGACAACC TTCGGGATGA AATGACCATC ATAAAGCAAA CTCCAGGTCA TAGGCGGAAC 
CCATCAAACC AAAGTAGCTT AGAATCTGAC TCCAATTACC CCTCCATCTC CACATCTGAG 
ATCGGAGACA CTGAGGATGC CCTCCAGCAG GTGGAGGAAA TTGGCCTGGA GAAGGCAGCC 
ATGGACATGA CGGTCTTCCT GAAGCTGCAG AAGAGAGTAC GGGAGCTGGA GCAGGAGAGG 
AAAAAGCTGC AAGTGCAGCT GGAGAAGAGA GAACAGCAGG ACAGCAAGAA AGTCCAGGCG 
GAACCACCAC AGACTGACAT AGATTTGGAC CCGAATGCAG ATCTGGCCTA CAATAGTCTG 
AAGAGGCAAG AGCTGGAGTC AGAGAACAAA AAGCTGAAGA ATGACCTGAA TGAGCTGAGG 
AAAGCCGTGG CCGACCAAGC CACGCAGAAT AACTCCAGCC ACGGCTCCCC AGATAGCTAC 
AGCCTCCTGC TGAACCAGCT CAAGCTGGCC CACGAGGAGC TCGAGGTGCG CAAGGAGGAG 
GTGCTCATCC TCAGGACCCA GATCGTGAGC GCCGACCAGC GGCGACTCGC CGGCAGGAAC 
GCGGAGCCGA ACATTAATGC CAGATCAAGT TGGCCTAACA GTGAAAAGCA TGTTGACCAG 
GAGGATGCCA TTGAGGCCTA TCACGGGGTC TGCCAGACAA ACAGCAAGAC TGAGGATTGG 
GGATATTTAA ATGAAGATGG AGAACTCGGC TTGGCCTACC AAGGCCTAAA GCAAGTTGCC 
AGGCTGCTGG AGGCTCAGCT GCAGGCCCAG AGCCTGGAGC ATGAGGAGGA GGTGGAGCAT 
CTCAAGGCTC AGCTCGAGGC CCTGAAGGAG GAGATGGACA AACAGCAGCA GACCTTCTGC 
CAGACGCTAC TGCTCTCCCC AGAGGCCCAG GTGGAATTCG GCGTTCAGCA GGAAATATCC 
CGGCTGACCA ACGAGAATCT GGACCTTAAA GAACTGGTAG AAAAGCTGGA AAAGAATGAG 
AGGAAGCTCA AAAAGCAACT GAAGATTTAC ATGAAGAAAG CCCAGGACCT AGAAGCTGCC 
CAGGCATTGG CCCAGAGTGA GAGGAAGCGC CATGAGCTCA ACAGGCAGGT CACGGTCCAG 
CGGAAAGAGA AGGATTTCCA GGGCATGCTG GAGTACCACA AAGAGGACGA GGCCCTCCTC 
ATCCGGAACC TGGTGACAGA CTTGAAGCCC CAGATGCTGT CGGGCACAGT GCCCTGTCTC 
CCCGCCTACA TCCTCTACAT GTGCATCCGG CACGCGGACT ACACCAACGA CGATCTCAAG 
GTGCACTCCC TGCTGACCTC CACCATCAAC GGCATTAAGA AAGTCCTGAA AAAGCACAAT 
GATGACTTTG AGATGACGTC ATTCTGGTTA TCCAACACCT GCCGCCTTCT TCACTGTCTG 
AAGCAGTACA GCGGGGATGA GGGCTTCATG ACTCAGAACA CTGCAAAGCA GAATGAACAC 
TGTCTTAAGA ATTTTGACCT CACCGAATAC CGTCAGGTGC TGAGTGACCT TTCCATTCAG 
ATCTACCAGC AGCTCATTAA AATTGCCGAG GGCGTGTTAC AGCCGATGAT AGTTTCTGCC 
ATGTTGGAAA ATGAGAGCAT TCAGGGTCTA TCTGGTGTGA AGCCCACCGG CTACCGGAAG 
CGCTCCTCCA GCATGGCAGA TGGGGATAAC TCATACTGCC TGGAAGCTAT CATCCGCCAG 
ATGAATGCCT TTCATACAGT CATGTGTGAC CAGGGCTTGG ACCCTGAGAT CATCCTGCAG 
GTATTCAAAC AGCTCTTCTA CATGATCAAC GCAGTGACTC TTAACAACCT GCTCTTGCGG 
AAGGACGTCT GCTCTTGGAG CACAGGCATG CAACTCAGGT ACAATATAAG TCAGCTTGAG 
GAGTGGCTTC GGGGAAGAAA CCTTCACCAG AGTGGAGCAG TTCAGACCAT GGAACCTCTG 
ATCCAAGCAG CCCAGCTCCT GCAATTAAAG AAGAAAACCC AGGAGGACGC AGAGGCTATC 
TGCTCCCTGT GTACCTCCCT CAGCACCCAG CAGATTGTCA AAATTTTAAA CCTTTATACT 
CCCCTGAATG AATTTGAAGA ACGGGTAACA GTGGCCTTTA TACGAACAAT CCAGGCACAA 
CTACAAGAGC GGAATGACCC TCAGCAACTG CTATTAGATG CCAAGCACAT GTTTCCTGTT 
TTGTTTCCAT TTAATCCATC TTCTCTAACC ATGGACTCAA TCCACATCCC AGCGTGTCTC 
AATCTGGAAT TCCTCAATGA AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human Rab small GTPase- and class V myosin-mediated membrane tethering in a chemically defined reconstitution system.
The Journal of biological chemistry292(45)18500-18517(2017 11)
Inoshita M,Mima J

Kinetic signatures of myosin-5B, the motor involved in microvillus inclusion disease.
The Journal of biological chemistry292(44)18372-18385(2017 11)
Heissler SM,Chinthalapudi K,Sellers JR

Defects in myosin VB are associated with a spectrum of previously undiagnosed low ?-glutamyltransferase cholestasis.
Hepatology (Baltimore, Md.)65(5)1655-1669(2017 05)
Qiu YL,Gong JY,Feng JY,Wang RX,Han J,Liu T,Lu Y,Li LT,Zhang MH,Sheps JA,Wang NL,Yan YY,Li JQ,Chen L,Borchers CH,Sipos B,Knisely AS,Ling V,Xing QH,Wang JS

MYO5B mutations cause cholestasis with normal serum gamma-glutamyl transferase activity in children without microvillous inclusion disease.
Hepatology (Baltimore, Md.)65(1)164-173(2017 01)
Gonzales E,Taylor SA,Davit-Spraul A,Th?baut A,Thomassin N,Guettier C,Whitington PF,Jacquemin E

Myosin Vb mediates Cu+ export in polarized hepatocytes.
Journal of cell science129(6)1179-89(2016 Mar)
Gupta A,Schell MJ,Bhattacharjee A,Lutsenko S,Hubbard AL

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MYO5B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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