myo5b cDNA ORF clone, Danio rerio(zebrafish)

The following myo5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myo5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa150935 XM_021468889.1
Latest version!
Danio rerio myosin VB (myo5b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa45150 XM_009295328.2
Latest version!
Danio rerio myosin VB (myo5b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa150932 XM_021468886.1
Latest version!
Danio rerio myosin VB (myo5b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa150933 XM_021468887.1
Latest version!
Danio rerio myosin VB (myo5b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa150934 XM_021468888.1
Latest version!
Danio rerio myosin VB (myo5b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa45151 NM_001161632.2
Latest version!
Danio rerio myosin VB (myo5b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa45148 XM_009295325.3
Latest version!
Danio rerio myosin VB (myo5b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa45149 XM_009295327.2
Latest version!
Danio rerio myosin VB (myo5b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
ODa45148 XM_009295325.2 Danio rerio myosin VB (myo5b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa150935
    Clone ID Related Accession (Same CDS sequence) XM_021468889.1
    Accession Version XM_021468889.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product unconventional myosin-Vb isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCTCTCT CTCGGGAGAT GTGCTCTATG CTCAAGAGAA AGATCCGGTT TGATTCTAGA 
    AGAGCTGTTC AGCAACTAAG AGCATGTGGT GTTCTGGAGA CCATTCGCAT TAGCGCCGCC
    GGCTATCCAT CACGATGGAC ATATCCAGAT TTCTTCAGCA GATATCGAGT GCTCATGACA
    AAGAAGGATC TGTCAGTCGG AGACAAGAAG CAGGTCTGTA AGAACCTGCT GGAGATCTTG
    ATCAAGGATC CAGATAAGTT CCAGTTTGGA AAGACTAAGA TCTTCTTCAG GGCGGGTCAG
    GTGGCCTATC TGGAGAAGCT CAGAGCCGAT AAGTTCCGCT TCGCCTGTAT TAAGATCCAG
    AAGACTGTGC GAGGATGGCT GCAGAGGATT CGGTATCGCA AGATTCGCAA GTCTGCCATC
    ACTTTACAAA GATACGGCCG GGGTTACTTG GCACGCAGGT ACGCAGAGAT GCTTCGCCTG
    ACCCGAGCAG CAGTCATTTG TCAGAAGCAG TACCGCATGG TACAGGTTCG CCGGGAGTAT
    CTGAGGGTCC GTCGGGCTGT AATCACCATT CAAGCATTCA CCCGAGGCAT GTTCATACGC
    AGGTTATATC AGGAGTTCCT GCTCCATCAC AAAGCCATGA TCATACAGAA GACTGTCCGT
    GGTTGGCTTG TAAGAAAGAA GTATCAGCGT TCCCGATACG CTGCCATCGT CATTCAGTGT
    TTCTATCGCC GCGTACGTGC CAAGCGGCAG CTCAAACAGC TCAAGATAGA GGCCCGTTCA
    GCCGAACACT TTAAAAAACT CAATGTCGGA ATGGAGAACA AGATCGTGCA GCTCCAGAAG
    AAGATGGACA ACCAGTCAAA AGAGTTAAAA TCTCAGAACG AAAATCTGGC TGTTGCTAAA
    ACTGTTCTGG AGACAGAGGT TAGTAAGCTG AGCAAAGAGC TGGAGACCCT CCGCACTCGA
    CAGGTGGCTG GCACTCAGAT GACATCACTG CAGGAAGAGC TGGAGAAACT GAGGGCGGAG
    CTTCAGGAAG CACATGCCCA CAAGAAACTT TTGGAGGAGG AGTTCAGCAA CGAGAAGCAA
    GGACTCGAAC AGAGGGTGGA GGAGCTTGAG AAAGAAAACA CAGTCTTGAA GAAGGAAAAA
    GAAGAGATGA ACCGCAGAAT CCAAACCTCT ACTCAGGATC AGGGAGGTGA TGTGTCTCAG
    AAGGAGAGTC GCCTGCAGCA TGAACTGGAT GACGAGAGAC AACGATATCA GAACCTGGTG
    AAAGAATATT CCCGACTGGA GCAGAGATAC GAAAACCTGC AGGAGGACAT GTCTTCAATG
    AAGTTTCATC CTGGGCATCG GAGAAACCCA TCTAACCAGA GCAGCTTGGG TTCAGACTCC
    AACTATCCCT CAATTAGCTT GTCAGAGGTG GGGGACACTG ATGACACCAT CCAGCAGGTG
    GAGGAGATGG GCATAGAGAA GGCTGCCATG GACCTCAGCG TTTTCATGAA GTTACAGAAA
    CGTGTACGAG AGCTGGAGCA GGAGAGGAAG CGGCTGCAGA CCAATCTAGA TAAAGTAGAG
    GAACTGAGCA AACGCAAGGG AAGCGAGGCC AAGAACTCAA CGTCAGAGGA CGATATAAAC
    GCAGACCTGG CCTACAACAG TCTGAAGCGG CAAGAGCTCG AAACAGAGAA TAAGAAGTTG
    AAGAATGACC TTAATGAGTT AAGGAAAGCA GTGGCCGACA GGGCCTCTGA GAACAACTCA
    TCCAATGAGC TTCAAGATGG CTACAACCTG CTGCTCAGTC AGCTCAAAGC GGCCAATGAG
    GAGCTGGATG TGCGCAAGGA GGAGGTCCTG ATGCTCAAGA CCCAGATCGT CAACTCAACA
    AGACAGACAG AAAACTTAAA CCACATCAAT TCCAACGATG TGTCTGAGTG GACCGACAAC
    AAGAGCCCTG TGAACACGGA GGACCGCGTG GATGCGCCTG CATCAAAGAA TAAAGCTCAA
    GACTGGGGTT ATTTGAATGA AGATGGCGAG CTAGGCCTGG CCTACCAGGG TCTAAAGCAG
    GTTGCCAGAC TGCTGGAGAC CCAACTGCAG ACCCAGACTC GACAGCACAG GGATGAGTTG
    GAAGCCCTTC ACACACAGAT CGAGCTCCTG AAGGACAATC TGGAGAAGAA GCAGGACATG
    CTGGACCACT TCTCCACACT TTCTCCTGAT GCTCTGGTGG AGTTCAGCGT GCAACAGGAG
    ATCACACGCC TCACCAATGA AAACCTGGAC CTAAAGGAAC TCGTTGAGAA GTTGGAAAAG
    AATGAGAAGA AGCTGAAGAA GCAGTTGAGG ATCTATATGA AGAAAGTGCA AGAGCTGGAA
    GTGACTCAGG CCACTAAAAG CAGACCGGAG CTCACTCGTC AGTTCACTGT CCAGAGAAGA
    GAGAAGGACT TTGAAGGCAT GCTGGAGTAC TATAAAGAAG ATGAGGCCTT GCTGGTCAAG
    ACTTTAGTTA CAGACATGAA GCCCAACGCT GTGTCCGCCA CTGTCCCCTG CCTCCCCGCA
    TACATCCTCT TCATGTGCAT CCGTCACGCC GACTACATCA ATGATGACCA GAAAGTTCAT
    TCACTACTGA CCGCTACCAT CAACGCCATC AAAAAAGTGC TGAAGAAAAA CAACGATGAC
    TTTGAGATGA CCTCCTTTTG GCTGGCAAAT GCAAGTCGAC TTTTACACTG TCTGAAGCAG
    TACAGCGGCG ATGAGGCGTT CATGACCCAG AACTCTGCCA AGCAGAACGA GCACTGCCTG
    AAGAACTTTG ACCTGGCGGA GTACCGACAG GTTCTGAGTG ACCTCTCCAT CCAAATCTAT
    CAGCAGCTTG TGAAAGTTGC CGAGGCCAAC ATGCAGCCCA TGATCGTGTC TGCCATGCTT
    GAGAGCGAGA GCATCCCCAG CCTGGCCGGA GTGAAGCCCA TGGGTTACAG GAACCGCTCC
    TCTAGTGTGG ACTGTGAGAG TGGAGGTCCC GCAGGATACA CGCTTCAGGC ACTGATCAAA
    CAGCTGGCTC AGTTTTACTC TACCATGGCG GACCACGGCC TGGACCCTGA GATCAGCCAG
    CAGGTGCTTC GCCAGCTCTT CTACTCCATC AACGCAGTGA CCCTCAACAA CCTGCTGCTG
    CGCAAGGACG TCTGCTCCTG GAGCACCGGC ATGCAGCTCA GGTACAACAT CAGTCAGCTG
    GAGGAGTGGC TAAGAGGGAA GAACCTGCAC CAGTGTGGAG CTGTGGCCAC TATGGAGCCG
    GTCATTCAGG CCGCGCAGCT GCTGCAGGTG AAGAAGAAGA CATCACAGGA CGCTGAAGCC
    ATCTGCTCGC TCTGCACTGC ACTCAGTTTA CAGCAGATTG TGAAGATTCT GAACTTGTAC
    ACACCACTCA ATGAGTTTGA GGAGCGAGTC ACTGTTTCCT TCATAAGAGA CATACAGAAT
    CGCCTCCAAG ATCGAATAGA AAACAACCAA CTGCTGGCGG ACACGAAATA CACCTTCCCC
    GTGCTCTTCC CGTACACTCC TTCCAACCTC AGCCTGGAAA CCATCCACAT CCCAGCCTCC
    CTCAGCCTGG ACTTCCTGAC GCGGGAGAAC TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324564.1
    CDS42..3617
    Translation

    Target ORF information:

    RefSeq Version XM_021468889.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio myosin VB (myo5b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468889.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCTCTCT CTCGGGAGAT GTGCTCTATG CTCAAGAGAA AGATCCGGTT TGATTCTAGA 
    AGAGCTGTTC AGCAACTAAG AGCATGTGGT GTTCTGGAGA CCATTCGCAT TAGCGCCGCC
    GGCTATCCAT CACGATGGAC ATATCCAGAT TTCTTCAGCA GATATCGAGT GCTCATGACA
    AAGAAGGATC TGTCAGTCGG AGACAAGAAG CAGGTCTGTA AGAACCTGCT GGAGATCTTG
    ATCAAGGATC CAGATAAGTT CCAGTTTGGA AAGACTAAGA TCTTCTTCAG GGCGGGTCAG
    GTGGCCTATC TGGAGAAGCT CAGAGCCGAT AAGTTCCGCT TCGCCTGTAT TAAGATCCAG
    AAGACTGTGC GAGGATGGCT GCAGAGGATT CGGTATCGCA AGATTCGCAA GTCTGCCATC
    ACTTTACAAA GATACGGCCG GGGTTACTTG GCACGCAGGT ACGCAGAGAT GCTTCGCCTG
    ACCCGAGCAG CAGTCATTTG TCAGAAGCAG TACCGCATGG TACAGGTTCG CCGGGAGTAT
    CTGAGGGTCC GTCGGGCTGT AATCACCATT CAAGCATTCA CCCGAGGCAT GTTCATACGC
    AGGTTATATC AGGAGTTCCT GCTCCATCAC AAAGCCATGA TCATACAGAA GACTGTCCGT
    GGTTGGCTTG TAAGAAAGAA GTATCAGCGT TCCCGATACG CTGCCATCGT CATTCAGTGT
    TTCTATCGCC GCGTACGTGC CAAGCGGCAG CTCAAACAGC TCAAGATAGA GGCCCGTTCA
    GCCGAACACT TTAAAAAACT CAATGTCGGA ATGGAGAACA AGATCGTGCA GCTCCAGAAG
    AAGATGGACA ACCAGTCAAA AGAGTTAAAA TCTCAGAACG AAAATCTGGC TGTTGCTAAA
    ACTGTTCTGG AGACAGAGGT TAGTAAGCTG AGCAAAGAGC TGGAGACCCT CCGCACTCGA
    CAGGTGGCTG GCACTCAGAT GACATCACTG CAGGAAGAGC TGGAGAAACT GAGGGCGGAG
    CTTCAGGAAG CACATGCCCA CAAGAAACTT TTGGAGGAGG AGTTCAGCAA CGAGAAGCAA
    GGACTCGAAC AGAGGGTGGA GGAGCTTGAG AAAGAAAACA CAGTCTTGAA GAAGGAAAAA
    GAAGAGATGA ACCGCAGAAT CCAAACCTCT ACTCAGGATC AGGGAGGTGA TGTGTCTCAG
    AAGGAGAGTC GCCTGCAGCA TGAACTGGAT GACGAGAGAC AACGATATCA GAACCTGGTG
    AAAGAATATT CCCGACTGGA GCAGAGATAC GAAAACCTGC AGGAGGACAT GTCTTCAATG
    AAGTTTCATC CTGGGCATCG GAGAAACCCA TCTAACCAGA GCAGCTTGGG TTCAGACTCC
    AACTATCCCT CAATTAGCTT GTCAGAGGTG GGGGACACTG ATGACACCAT CCAGCAGGTG
    GAGGAGATGG GCATAGAGAA GGCTGCCATG GACCTCAGCG TTTTCATGAA GTTACAGAAA
    CGTGTACGAG AGCTGGAGCA GGAGAGGAAG CGGCTGCAGA CCAATCTAGA TAAAGTAGAG
    GAACTGAGCA AACGCAAGGG AAGCGAGGCC AAGAACTCAA CGTCAGAGGA CGATATAAAC
    GCAGACCTGG CCTACAACAG TCTGAAGCGG CAAGAGCTCG AAACAGAGAA TAAGAAGTTG
    AAGAATGACC TTAATGAGTT AAGGAAAGCA GTGGCCGACA GGGCCTCTGA GAACAACTCA
    TCCAATGAGC TTCAAGATGG CTACAACCTG CTGCTCAGTC AGCTCAAAGC GGCCAATGAG
    GAGCTGGATG TGCGCAAGGA GGAGGTCCTG ATGCTCAAGA CCCAGATCGT CAACTCAACA
    AGACAGACAG AAAACTTAAA CCACATCAAT TCCAACGATG TGTCTGAGTG GACCGACAAC
    AAGAGCCCTG TGAACACGGA GGACCGCGTG GATGCGCCTG CATCAAAGAA TAAAGCTCAA
    GACTGGGGTT ATTTGAATGA AGATGGCGAG CTAGGCCTGG CCTACCAGGG TCTAAAGCAG
    GTTGCCAGAC TGCTGGAGAC CCAACTGCAG ACCCAGACTC GACAGCACAG GGATGAGTTG
    GAAGCCCTTC ACACACAGAT CGAGCTCCTG AAGGACAATC TGGAGAAGAA GCAGGACATG
    CTGGACCACT TCTCCACACT TTCTCCTGAT GCTCTGGTGG AGTTCAGCGT GCAACAGGAG
    ATCACACGCC TCACCAATGA AAACCTGGAC CTAAAGGAAC TCGTTGAGAA GTTGGAAAAG
    AATGAGAAGA AGCTGAAGAA GCAGTTGAGG ATCTATATGA AGAAAGTGCA AGAGCTGGAA
    GTGACTCAGG CCACTAAAAG CAGACCGGAG CTCACTCGTC AGTTCACTGT CCAGAGAAGA
    GAGAAGGACT TTGAAGGCAT GCTGGAGTAC TATAAAGAAG ATGAGGCCTT GCTGGTCAAG
    ACTTTAGTTA CAGACATGAA GCCCAACGCT GTGTCCGCCA CTGTCCCCTG CCTCCCCGCA
    TACATCCTCT TCATGTGCAT CCGTCACGCC GACTACATCA ATGATGACCA GAAAGTTCAT
    TCACTACTGA CCGCTACCAT CAACGCCATC AAAAAAGTGC TGAAGAAAAA CAACGATGAC
    TTTGAGATGA CCTCCTTTTG GCTGGCAAAT GCAAGTCGAC TTTTACACTG TCTGAAGCAG
    TACAGCGGCG ATGAGGCGTT CATGACCCAG AACTCTGCCA AGCAGAACGA GCACTGCCTG
    AAGAACTTTG ACCTGGCGGA GTACCGACAG GTTCTGAGTG ACCTCTCCAT CCAAATCTAT
    CAGCAGCTTG TGAAAGTTGC CGAGGCCAAC ATGCAGCCCA TGATCGTGTC TGCCATGCTT
    GAGAGCGAGA GCATCCCCAG CCTGGCCGGA GTGAAGCCCA TGGGTTACAG GAACCGCTCC
    TCTAGTGTGG ACTGTGAGAG TGGAGGTCCC GCAGGATACA CGCTTCAGGC ACTGATCAAA
    CAGCTGGCTC AGTTTTACTC TACCATGGCG GACCACGGCC TGGACCCTGA GATCAGCCAG
    CAGGTGCTTC GCCAGCTCTT CTACTCCATC AACGCAGTGA CCCTCAACAA CCTGCTGCTG
    CGCAAGGACG TCTGCTCCTG GAGCACCGGC ATGCAGCTCA GGTACAACAT CAGTCAGCTG
    GAGGAGTGGC TAAGAGGGAA GAACCTGCAC CAGTGTGGAG CTGTGGCCAC TATGGAGCCG
    GTCATTCAGG CCGCGCAGCT GCTGCAGGTG AAGAAGAAGA CATCACAGGA CGCTGAAGCC
    ATCTGCTCGC TCTGCACTGC ACTCAGTTTA CAGCAGATTG TGAAGATTCT GAACTTGTAC
    ACACCACTCA ATGAGTTTGA GGAGCGAGTC ACTGTTTCCT TCATAAGAGA CATACAGAAT
    CGCCTCCAAG ATCGAATAGA AAACAACCAA CTGCTGGCGG ACACGAAATA CACCTTCCCC
    GTGCTCTTCC CGTACACTCC TTCCAACCTC AGCCTGGAAA CCATCCACAT CCCAGCCTCC
    CTCAGCCTGG ACTTCCTGAC GCGGGAGAAC TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45150
    Clone ID Related Accession (Same CDS sequence) XM_009295328.2
    Accession Version XM_009295328.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product unconventional myosin-Vb isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCTCTCT CTCGGGAGAT GTGCTCTATG CTCAAGAGAA AGATCCGGTT TGATTCTAGA 
    AGAGCTGTTC AGCAACTAAG AGCATGTGGT GTTCTGGAGA CCATTCGCAT TAGCGCCGCC
    GGCTATCCAT CACGATGGAC ATATCCAGAT TTCTTCAGCA GATATCGAGT GCTCATGACA
    AAGAAGGATC TGTCAGTCGG AGACAAGAAG CAGGTCTGTA AGAACCTGCT GGAGATCTTG
    ATCAAGGATC CAGATAAGTT CCAGTTTGGA AAGACTAAGA TCTTCTTCAG GGCGGGTCAG
    GTGGCCTATC TGGAGAAGCT CAGAGCCGAT AAGTTCCGCT TCGCCTGTAT TAAGATCCAG
    AAGACTGTGC GAGGATGGCT GCAGAGGATT CGGTATCGCA AGATTCGCAA GTCTGCCATC
    ACTTTACAAA GATACGGCCG GGGTTACTTG GCACGCAGGT ACGCAGAGAT GCTTCGCCTG
    ACCCGAGCAG CAGTCATTTG TCAGAAGCAG TACCGCATGG TACAGGTTCG CCGGGAGTAT
    CTGAGGGTCC GTCGGGCTGT AATCACCATT CAAGCATTCA CCCGAGGCAT GTTCATACGC
    AGGTTATATC AGGAGTTCCT GCTCCATCAC AAAGCCATGA TCATACAGAA GACTGTCCGT
    GGTTGGCTTG TAAGAAAGAA GTATCAGCGT TCCCGATACG CTGCCATCGT CATTCAGTGT
    TTCTATCGCC GCGTACGTGC CAAGCGGCAG CTCAAACAGC TCAAGATAGA GGCCCGTTCA
    GCCGAACACT TTAAAAAACT CAATGTCGGA ATGGAGAACA AGATCGTGCA GCTCCAGAAG
    AAGATGGACA ACCAGTCAAA AGAGTTAAAA TCTCAGAACG AAAATCTGGC TGTTGCTAAA
    ACTGTTCTGG AGACAGAGGT TAGTAAGCTG AGCAAAGAGC TGGAGACCCT CCGCACTCGA
    CAGGTGGCTG GCACTCAGAT GACATCACTG CAGGAAGAGC TGGAGAAACT GAGGGCGGAG
    CTTCAGGAAG CACATGCCCA CAAGAAACTT TTGGAGGAGG AGTTCAGCAA CGAGAAGCAA
    GGACTCGAAC AGAGGGTGGA GGAGCTTGAG AAAGAAAACA CAGTCTTGAA GAAGGAAAAA
    GAAGAGATGA ACCGCAGAAT CCAAACCTCT ACTCAGGATC AGGGAGGTGA TGTGTCTCAG
    AAGGAGAGTC GCCTGCAGCA TGAACTGGAT GACGAGAGAC AACGATATCA GAACCTGGTG
    AAAGAATATT CCCGACTGGA GCAGAGATAC GAAAACCTGC AGGAGGACAT GTCTTCAATG
    AAGTTTCATC CTGGGCATCG GAGAAACCCA TCTAACCAGA GCAGCTTGGG TTCAGACTCC
    AACTATCCCT CAATTAGCTT GTCAGAGGTG GGGGACACTG ATGACACCAT CCAGCAGGTG
    GAGGAGATGG GCATAGAGAA GGCTGCCATG GACCTCAGCG TTTTCATGAA GTTACAGAAA
    CGTGTACGAG AGCTGGAGCA GGAGAGGAAG CGGCTGCAGA CCAATCTAGA TAAAGTAGAG
    GAACTGAGCA AACGCAAGGG AAGCGAGGCC AAGAACTCAA CGTCAGAGGA CGATATAAAC
    GCAGACCTGG CCTACAACAG TCTGAAGCGG CAAGAGCTCG AAACAGAGAA TAAGAAGTTG
    AAGAATGACC TTAATGAGTT AAGGAAAGCA GTGGCCGACA GGGCCTCTGA GAACAACTCA
    TCCAATGAGC TTCAAGATGG CTACAACCTG CTGCTCAGTC AGCTCAAAGC GGCCAATGAG
    GAGCTGGATG TGCGCAAGGA GGAGGTCCTG ATGCTCAAGA CCCAGATCGT CAACTCAACA
    AGACAGACAG AAAACTTAAA CCACATCAAT TCCAACGATG TGTCTGAGTG GACCGACAAC
    AAGAGCCCTG TGAACACGGA GGACCGCGTG GATGCGCCTG CATCAAAGAA TAAAGCTCAA
    GACTGGGGTT ATTTGAATGA AGATGGCGAG CTAGGCCTGG CCTACCAGGG TCTAAAGCAG
    GTTGCCAGAC TGCTGGAGAC CCAACTGCAG ACCCAGACTC GACAGCACAG GGATGAGTTG
    GAAGCCCTTC ACACACAGAT CGAGCTCCTG AAGGACAATC TGGAGAAGAA GCAGGACATG
    CTGGACCACT TCTCCACACT TTCTCCTGAT GCTCTGGTGG AGTTCAGCGT GCAACAGGAG
    ATCACACGCC TCACCAATGA AAACCTGGAC CTAAAGGAAC TCGTTGAGAA GTTGGAAAAG
    AATGAGAAGA AGCTGAAGAA GCAGTTGAGG ATCTATATGA AGAAAGTGCA AGAGCTGGAA
    GTGACTCAGG CCACTAAAAG CAGACCGGAG CTCACTCGTC AGTTCACTGT CCAGAGAAGA
    GAGAAGGACT TTGAAGGCAT GCTGGAGTAC TATAAAGAAG ATGAGGCCTT GCTGGTCAAG
    ACTTTAGTTA CAGACATGAA GCCCAACGCT GTGTCCGCCA CTGTCCCCTG CCTCCCCGCA
    TACATCCTCT TCATGTGCAT CCGTCACGCC GACTACATCA ATGATGACCA GAAAGTTCAT
    TCACTACTGA CCGCTACCAT CAACGCCATC AAAAAAGTGC TGAAGAAAAA CAACGATGAC
    TTTGAGATGA CCTCCTTTTG GCTGGCAAAT GCAAGTCGAC TTTTACACTG TCTGAAGCAG
    TACAGCGGCG ATGAGGCGTT CATGACCCAG AACTCTGCCA AGCAGAACGA GCACTGCCTG
    AAGAACTTTG ACCTGGCGGA GTACCGACAG GTTCTGAGTG ACCTCTCCAT CCAAATCTAT
    CAGCAGCTTG TGAAAGTTGC CGAGGCCAAC ATGCAGCCCA TGATCGTGTC TGCCATGCTT
    GAGAGCGAGA GCATCCCCAG CCTGGCCGGA GTGAAGCCCA TGGGTTACAG GAACCGCTCC
    TCTAGTGTGG ACTGTGAGAG TGGAGGTCCC GCAGGATACA CGCTTCAGGC ACTGATCAAA
    CAGCTGGCTC AGTTTTACTC TACCATGGCG GACCACGGCC TGGACCCTGA GATCAGCCAG
    CAGGTGCTTC GCCAGCTCTT CTACTCCATC AACGCAGTGA CCCTCAACAA CCTGCTGCTG
    CGCAAGGACG TCTGCTCCTG GAGCACCGGC ATGCAGCTCA GGTACAACAT CAGTCAGCTG
    GAGGAGTGGC TAAGAGGGAA GAACCTGCAC CAGTGTGGAG CTGTGGCCAC TATGGAGCCG
    GTCATTCAGG CCGCGCAGCT GCTGCAGGTG AAGAAGAAGA CATCACAGGA CGCTGAAGCC
    ATCTGCTCGC TCTGCACTGC ACTCAGTTTA CAGCAGATTG TGAAGATTCT GAACTTGTAC
    ACACCACTCA ATGAGTTTGA GGAGCGAGTC ACTGTTTCCT TCATAAGAGA CATACAGAAT
    CGCCTCCAAG ATCGAATAGA AAACAACCAA CTGCTGGCGG ACACGAAATA CACCTTCCCC
    GTGCTCTTCC CGTACACTCC TTCCAACCTC AGCCTGGAAA CCATCCACAT CCCAGCCTCC
    CTCAGCCTGG ACTTCCTGAC GCGGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293603.1
    CDS40..3609
    Translation

    Target ORF information:

    RefSeq Version XM_009295328.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio myosin VB (myo5b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295328.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCTCTCT CTCGGGAGAT GTGCTCTATG CTCAAGAGAA AGATCCGGTT TGATTCTAGA 
    AGAGCTGTTC AGCAACTAAG AGCATGTGGT GTTCTGGAGA CCATTCGCAT TAGCGCCGCC
    GGCTATCCAT CACGATGGAC ATATCCAGAT TTCTTCAGCA GATATCGAGT GCTCATGACA
    AAGAAGGATC TGTCAGTCGG AGACAAGAAG CAGGTCTGTA AGAACCTGCT GGAGATCTTG
    ATCAAGGATC CAGATAAGTT CCAGTTTGGA AAGACTAAGA TCTTCTTCAG GGCGGGTCAG
    GTGGCCTATC TGGAGAAGCT CAGAGCCGAT AAGTTCCGCT TCGCCTGTAT TAAGATCCAG
    AAGACTGTGC GAGGATGGCT GCAGAGGATT CGGTATCGCA AGATTCGCAA GTCTGCCATC
    ACTTTACAAA GATACGGCCG GGGTTACTTG GCACGCAGGT ACGCAGAGAT GCTTCGCCTG
    ACCCGAGCAG CAGTCATTTG TCAGAAGCAG TACCGCATGG TACAGGTTCG CCGGGAGTAT
    CTGAGGGTCC GTCGGGCTGT AATCACCATT CAAGCATTCA CCCGAGGCAT GTTCATACGC
    AGGTTATATC AGGAGTTCCT GCTCCATCAC AAAGCCATGA TCATACAGAA GACTGTCCGT
    GGTTGGCTTG TAAGAAAGAA GTATCAGCGT TCCCGATACG CTGCCATCGT CATTCAGTGT
    TTCTATCGCC GCGTACGTGC CAAGCGGCAG CTCAAACAGC TCAAGATAGA GGCCCGTTCA
    GCCGAACACT TTAAAAAACT CAATGTCGGA ATGGAGAACA AGATCGTGCA GCTCCAGAAG
    AAGATGGACA ACCAGTCAAA AGAGTTAAAA TCTCAGAACG AAAATCTGGC TGTTGCTAAA
    ACTGTTCTGG AGACAGAGGT TAGTAAGCTG AGCAAAGAGC TGGAGACCCT CCGCACTCGA
    CAGGTGGCTG GCACTCAGAT GACATCACTG CAGGAAGAGC TGGAGAAACT GAGGGCGGAG
    CTTCAGGAAG CACATGCCCA CAAGAAACTT TTGGAGGAGG AGTTCAGCAA CGAGAAGCAA
    GGACTCGAAC AGAGGGTGGA GGAGCTTGAG AAAGAAAACA CAGTCTTGAA GAAGGAAAAA
    GAAGAGATGA ACCGCAGAAT CCAAACCTCT ACTCAGGATC AGGGAGGTGA TGTGTCTCAG
    AAGGAGAGTC GCCTGCAGCA TGAACTGGAT GACGAGAGAC AACGATATCA GAACCTGGTG
    AAAGAATATT CCCGACTGGA GCAGAGATAC GAAAACCTGC AGGAGGACAT GTCTTCAATG
    AAGTTTCATC CTGGGCATCG GAGAAACCCA TCTAACCAGA GCAGCTTGGG TTCAGACTCC
    AACTATCCCT CAATTAGCTT GTCAGAGGTG GGGGACACTG ATGACACCAT CCAGCAGGTG
    GAGGAGATGG GCATAGAGAA GGCTGCCATG GACCTCAGCG TTTTCATGAA GTTACAGAAA
    CGTGTACGAG AGCTGGAGCA GGAGAGGAAG CGGCTGCAGA CCAATCTAGA TAAAGTAGAG
    GAACTGAGCA AACGCAAGGG AAGCGAGGCC AAGAACTCAA CGTCAGAGGA CGATATAAAC
    GCAGACCTGG CCTACAACAG TCTGAAGCGG CAAGAGCTCG AAACAGAGAA TAAGAAGTTG
    AAGAATGACC TTAATGAGTT AAGGAAAGCA GTGGCCGACA GGGCCTCTGA GAACAACTCA
    TCCAATGAGC TTCAAGATGG CTACAACCTG CTGCTCAGTC AGCTCAAAGC GGCCAATGAG
    GAGCTGGATG TGCGCAAGGA GGAGGTCCTG ATGCTCAAGA CCCAGATCGT CAACTCAACA
    AGACAGACAG AAAACTTAAA CCACATCAAT TCCAACGATG TGTCTGAGTG GACCGACAAC
    AAGAGCCCTG TGAACACGGA GGACCGCGTG GATGCGCCTG CATCAAAGAA TAAAGCTCAA
    GACTGGGGTT ATTTGAATGA AGATGGCGAG CTAGGCCTGG CCTACCAGGG TCTAAAGCAG
    GTTGCCAGAC TGCTGGAGAC CCAACTGCAG ACCCAGACTC GACAGCACAG GGATGAGTTG
    GAAGCCCTTC ACACACAGAT CGAGCTCCTG AAGGACAATC TGGAGAAGAA GCAGGACATG
    CTGGACCACT TCTCCACACT TTCTCCTGAT GCTCTGGTGG AGTTCAGCGT GCAACAGGAG
    ATCACACGCC TCACCAATGA AAACCTGGAC CTAAAGGAAC TCGTTGAGAA GTTGGAAAAG
    AATGAGAAGA AGCTGAAGAA GCAGTTGAGG ATCTATATGA AGAAAGTGCA AGAGCTGGAA
    GTGACTCAGG CCACTAAAAG CAGACCGGAG CTCACTCGTC AGTTCACTGT CCAGAGAAGA
    GAGAAGGACT TTGAAGGCAT GCTGGAGTAC TATAAAGAAG ATGAGGCCTT GCTGGTCAAG
    ACTTTAGTTA CAGACATGAA GCCCAACGCT GTGTCCGCCA CTGTCCCCTG CCTCCCCGCA
    TACATCCTCT TCATGTGCAT CCGTCACGCC GACTACATCA ATGATGACCA GAAAGTTCAT
    TCACTACTGA CCGCTACCAT CAACGCCATC AAAAAAGTGC TGAAGAAAAA CAACGATGAC
    TTTGAGATGA CCTCCTTTTG GCTGGCAAAT GCAAGTCGAC TTTTACACTG TCTGAAGCAG
    TACAGCGGCG ATGAGGCGTT CATGACCCAG AACTCTGCCA AGCAGAACGA GCACTGCCTG
    AAGAACTTTG ACCTGGCGGA GTACCGACAG GTTCTGAGTG ACCTCTCCAT CCAAATCTAT
    CAGCAGCTTG TGAAAGTTGC CGAGGCCAAC ATGCAGCCCA TGATCGTGTC TGCCATGCTT
    GAGAGCGAGA GCATCCCCAG CCTGGCCGGA GTGAAGCCCA TGGGTTACAG GAACCGCTCC
    TCTAGTGTGG ACTGTGAGAG TGGAGGTCCC GCAGGATACA CGCTTCAGGC ACTGATCAAA
    CAGCTGGCTC AGTTTTACTC TACCATGGCG GACCACGGCC TGGACCCTGA GATCAGCCAG
    CAGGTGCTTC GCCAGCTCTT CTACTCCATC AACGCAGTGA CCCTCAACAA CCTGCTGCTG
    CGCAAGGACG TCTGCTCCTG GAGCACCGGC ATGCAGCTCA GGTACAACAT CAGTCAGCTG
    GAGGAGTGGC TAAGAGGGAA GAACCTGCAC CAGTGTGGAG CTGTGGCCAC TATGGAGCCG
    GTCATTCAGG CCGCGCAGCT GCTGCAGGTG AAGAAGAAGA CATCACAGGA CGCTGAAGCC
    ATCTGCTCGC TCTGCACTGC ACTCAGTTTA CAGCAGATTG TGAAGATTCT GAACTTGTAC
    ACACCACTCA ATGAGTTTGA GGAGCGAGTC ACTGTTTCCT TCATAAGAGA CATACAGAAT
    CGCCTCCAAG ATCGAATAGA AAACAACCAA CTGCTGGCGG ACACGAAATA CACCTTCCCC
    GTGCTCTTCC CGTACACTCC TTCCAACCTC AGCCTGGAAA CCATCCACAT CCCAGCCTCC
    CTCAGCCTGG ACTTCCTGAC GCGGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150932
    Clone ID Related Accession (Same CDS sequence) XM_021468886.1
    Accession Version XM_021468886.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product unconventional myosin-Vb isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTCTGTAA CTGAACTCTA CTCTAAGTAC ACCCGGGTTT GGATTCCTGA CCCGGATGAG 
    GTCTGGAGGT CTGCTGAAAT CATCAAGGAT TATAAGGAGG GAGACACAGT GCTGCATCTC
    AAATTAGAAG ATGAATCGAC GTTAGAATAT CCAATTGGTT CCAAAGACAA TCCGCTTCCA
    TTTCTCCGGA ATCCCGATAT CCTGGTAGGA GAGAATGACC TCACGGCTCT CAGTTACCTC
    CATGAGCCTG CTGTGCTCCA CAACCTCAAG GTCCGCTTCC TCGAGTCCAA CCACATCTAC
    ACCTATTGTG GGATTGTTCT GGTGGCCATC AACCCGTACG AGCAGCTACA TATCTATGGA
    GAAGAAGTCA TCAATGCATA CAGCGGGCAG AACATGGGGG ACATGGACCC TCATATATTT
    GCGGTGGCCG AGGAAGCTTT TAAACAGATG GCCAGAGATG AGAAGAACCA GTCCATAATT
    GTGAGCGGTG AGTCGGGAGC TGGTAAAACC GTTTCGGCAA AGTATGCCAT GCGCTTCTTC
    GCCACAGTGG GAGGATCAGC AAATGACACC AATGTTGAAG AGAAAGTTTT GGCTTCCAGT
    CCCATTATGG AGGCCATTGG AAATGCAAAA ACAACCCGTA ATGACAACAG CAGCCGTTTT
    GGGAAATACA TCCAGATCGG CTTTGACAGG AGATATCATA TTATTGGTGC CAATATGAGA
    ACGTACCTGC TTGAGAAGTC CAGAGTAGTG TTTCAGGCTG AAGAGGAGAG GAACTACCAC
    ATCTTCTACC AGCTCTGTGC TTCATGCTCT CTGCCAGAGT TTAAGGATCT TACCTTGACC
    AGTGCAGAAG ACTTCACTTA CACGTCTCTC GGTGAGAACA TTTTCATTGA GGGAGTGAAT
    GATGCCGAGG ACCTTGTGAA GACGAGAGAA GCTCTTACGA TGCTTGGTGT AAAAGAAAAC
    CACCAAATGA GCATCTTTAA GATAATTGCC TCCATTTTGC ACCTGGGGAA CGTGGAGATT
    GTGCAGGAGA GAGATGGAGA ATCCTGCCAT ATCAATAGGA ACGACACTCA TCTGCATCAT
    TTCTGTCGGC TGTTGGGGAT TGAGCAGGAG CAGATGGAGA ACTGGCTATG CCGCAGGAAA
    CTGGTGACCA CTTCTGAAAC TTATGTGAAA AACATGCCCC ACAGCCAGGC AGTCAATGCC
    CGAGATGCCT TGGCCAAACA CATCTATGCC CATCTGTTTG ACTGGATCGT GGAGCACATC
    AACAAGTCCC TGCATACTTC CACTAAACAG CACTCATTCA TCGGGGTTCT GGATATCTAT
    GGGTTTGAAA CATTTGAGAT CAACAGCTTT GAGCAGTTCT GTATTAACTA CGCCAATGAG
    AAGCTACAGC AGCAATTCAA CTCGCATGTG TTTAAGCTGG AGCAAGAAGA GTACATGAAG
    GAGCAGATCC CCTGGACACT CATTGATTTC TATGATAACC AGCCGTGCAT TGACCTCATT
    GAAGCCAAAC TCGGTATCCT GGACCTTCTT GATGAAGAGT GTAAGGTGCC CAAAGGAACA
    GATCAGAACT GGGCGCAAAA GCTGTACAGC AAGCATTCAG GCAGCGGCCA TTTCGAAAAG
    CCCAGGATGT CCAACAAGTC CTTTATTGTT GTTCATTTTG CAGATAAGGT TGAATATCAG
    TGTGACGGAT TCCTGGAGAA GAACAGAGAC ACTGTTTATG AAGAGCAGAT AAACATCCTC
    AAGGCCAGTA AGTTCCAGTT GGTTGCAGAT CTCTTTGACG ATGCTAAAGA TGCTGCCCCT
    CATCCAGGCA GCAAAACCTC CAGAATCAAT GTTCGGCCTG CCAAACCCGC TCCGAAAGGT
    CACAACCGGG AGCACAGGAA AACTGTGGGC ACTCAGTTCA GAAACTCTCT GCATCTTCTG
    ATGGAGACAC TCAACGCAAC CACCCCTCAT TATGTTCGCT GCATTAAGCC CAATGATTAT
    AAGGAATCCT TTGTGTTTGA TTCTAGAAGA GCTGTTCAGC AACTAAGAGC ATGTGGTGTT
    CTGGAGACCA TTCGCATTAG CGCCGCCGGC TATCCATCAC GATGGACATA TCCAGATTTC
    TTCAGCAGAT ATCGAGTGCT CATGACAAAG AAGGATCTGT CAGTCGGAGA CAAGAAGCAG
    GTCTGTAAGA ACCTGCTGGA GATCTTGATC AAGGATCCAG ATAAGTTCCA GTTTGGAAAG
    ACTAAGATCT TCTTCAGGGC GGGTCAGGTG GCCTATCTGG AGAAGCTCAG AGCCGATAAG
    TTCCGCTTCG CCTGTATTAA GATCCAGAAG ACTGTGCGAG GATGGCTGCA GAGGATTCGG
    TATCGCAAGA TTCGCAAGTC TGCCATCACT TTACAAAGAT ACGGCCGGGG TTACTTGGCA
    CGCAGGTACG CAGAGATGCT TCGCCTGACC CGAGCAGCAG TCATTTGTCA GAAGCAGTAC
    CGCATGGTAC AGGTTCGCCG GGAGTATCTG AGGGTCCGTC GGGCTGTAAT CACCATTCAA
    GCATTCACCC GAGGCATGTT CATACGCAGG TTATATCAGG AGTTCCTGCT CCATCACAAA
    GCCATGATCA TACAGAAGAC TGTCCGTGGT TGGCTTGTAA GAAAGAAGTA TCAGCGTTCC
    CGATACGCTG CCATCGTCAT TCAGTGTTTC TATCGCCGCG TACGTGCCAA GCGGCAGCTC
    AAACAGCTCA AGATAGAGGC CCGTTCAGCC GAACACTTTA AAAAACTCAA TGTCGGAATG
    GAGAACAAGA TCGTGCAGCT CCAGAAGAAG ATGGACAACC AGTCAAAAGA GTTAAAATCT
    CAGAACGAAA ATCTGGCTGT TGCTAAAACT GTTCTGGAGA CAGAGGTTAG TAAGCTGAGC
    AAAGAGCTGG AGACCCTCCG CACTCGACAG GTGGCTGGCA CTCAGATGAC ATCACTGCAG
    GAAGAGCTGG AGAAACTGAG GGCGGAGCTT CAGGAAGCAC ATGCCCACAA GAAACTTTTG
    GAGGAGGAGT TCAGCAACGA GAAGCAAGGA CTCGAACAGA GGGTGGAGGA GCTTGAGAAA
    GAAAACACAG TCTTGAAGAA GGAAAAAGAA GAGATGAACC GCAGAATCCA AACCTCTACT
    CAGGATCAGG GAGGTGATGT GTCTCAGAAG GAGAGTCGCC TGCAGCATGA ACTGGATGAC
    GAGAGACAAC GATATCAGAA CCTGGTGAAA GAATATTCCC GACTGGAGCA GAGATACGAA
    AACCTGCAGG AGGACATGTC TTCAATGAAG TTTCATCCTG GGCATCGGAG AAACCCATCT
    AACCAGAGCA GCTTGGGTTC AGACTCCAAC TATCCCTCAA TTAGCTTGTC AGAGGTGGGG
    GACACTGATG ACACCATCCA GCAGGTGGAG GAGATGGGCA TAGAGAAGGC TGCCATGGAC
    CTCAGCGTTT TCATGAAGTT ACAGAAACGT GTACGAGAGC TGGAGCAGGA GAGGAAGCGG
    CTGCAGACCA ATCTAGATAA AGTAGAGGAA CTGAGCAAAC GCAAGGGAAG CGAGGCCAAG
    AACTCAACGT CAGAGGACGA TATAAACGCA GACCTGGCCT ACAACAGTCT GAAGCGGCAA
    GAGCTCGAAA CAGAGAATAA GAAGTTGAAG AATGACCTTA ATGAGTTAAG GAAAGCAGTG
    GCCGACAGGG CCTCTGAGAA CAACTCATCC AATGAGCTTC AAGATGGCTA CAACCTGCTG
    CTCAGTCAGC TCAAAGCGGC CAATGAGGAG CTGGATGTGC GCAAGGAGGA GGTCCTGATG
    CTCAAGACCC AGATCGTCAA CTCAACAAGA CAGACAGAAA ACTTAAACCA CATCAATTCC
    AACGATGTGT CTGAGTGGAC CGACAACAAG AGCCCTGTGA ACACGGAGGA CCGCGTGGAT
    GCGCCTGCAT CAAAGAATAA AGCTCAAGAC TGGGGTTATT TGAATGAAGA TGGCGAGCTA
    GGCCTGGCCT ACCAGGGTCT AAAGCAGGTT GCCAGACTGC TGGAGACCCA ACTGCAGACC
    CAGACTCGAC AGCACAGGGA TGAGTTGGAA GCCCTTCACA CACAGATCGA GCTCCTGAAG
    GACAATCTGG AGAAGAAGCA GGACATGCTG GACCACTTCT CCACACTTTC TCCTGATGCT
    CTGGTGGAGT TCAGCGTGCA ACAGGAGATC ACACGCCTCA CCAATGAAAA CCTGGACCTA
    AAGGAACTCG TTGAGAAGTT GGAAAAGAAT GAGAAGAAGC TGAAGAAGCA GTTGAGGATC
    TATATGAAGA AAGTGCAAGA GCTGGAAGTG ACTCAGGCCA CTAAAAGCAG ACCGGAGCTC
    ACTCGTCAGT TCACTGTCCA GAGAAGAGAG AAGGACTTTG AAGGCATGCT GGAGTACTAT
    AAAGAAGATG AGGCCTTGCT GGTCAAGACT TTAGTTACAG ACATGAAGCC CAACGCTGTG
    TCCGCCACTG TCCCCTGCCT CCCCGCATAC ATCCTCTTCA TGTGCATCCG TCACGCCGAC
    TACATCAATG ATGACCAGAA AGTTCATTCA CTACTGACCG CTACCATCAA CGCCATCAAA
    AAAGTGCTGA AGAAAAACAA CGATGACTTT GAGATGACCT CCTTTTGGCT GGCAAATGCA
    AGTCGACTTT TACACTGTCT GAAGCAGTAC AGCGGCGATG AGGCGTTCAT GACCCAGAAC
    TCTGCCAAGC AGAACGAGCA CTGCCTGAAG AACTTTGACC TGGCGGAGTA CCGACAGGTT
    CTGAGTGACC TCTCCATCCA AATCTATCAG CAGCTTGTGA AAGTTGCCGA GGCCAACATG
    CAGCCCATGA TCGTGTCTGC CATGCTTGAG AGCGAGAGCA TCCCCAGCCT GGCCGGAGTG
    AAGCCCATGG GTTACAGGAA CCGCTCCTCT AGTGTGGACT GTGAGAGTGG AGGTCCCGCA
    GGATACACGC TTCAGGCACT GATCAAACAG CTGGCTCAGT TTTACTCTAC CATGGCGGAC
    CACGGCCTGG ACCCTGAGAT CAGCCAGCAG GTGCTTCGCC AGCTCTTCTA CTCCATCAAC
    GCAGTGACCC TCAACAACCT GCTGCTGCGC AAGGACGTCT GCTCCTGGAG CACCGGCATG
    CAGCTCAGGT ACAACATCAG TCAGCTGGAG GAGTGGCTAA GAGGGAAGAA CCTGCACCAG
    TGTGGAGCTG TGGCCACTAT GGAGCCGGTC ATTCAGGCCG CGCAGCTGCT GCAGGTGAAG
    AAGAAGACAT CACAGGACGC TGAAGCCATC TGCTCGCTCT GCACTGCACT CAGTTTACAG
    CAGATTGTGA AGATTCTGAA CTTGTACACA CCACTCAATG AGTTTGAGGA GCGAGTCACT
    GTTTCCTTCA TAAGAGACAT ACAGAATCGC CTCCAAGATC GAATAGAAAA CAACCAACTG
    CTGGCGGACA CGAAATACAC CTTCCCCGTG CTCTTCCCGT ACACTCCTTC CAACCTCAGC
    CTGGAAACCA TCCACATCCC AGCCTCCCTC AGCCTGGACT TCCTGACGCG GGAGAACTTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324561.1
    CDS460..5982
    Translation

    Target ORF information:

    RefSeq Version XM_021468886.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio myosin VB (myo5b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTCTGTAA CTGAACTCTA CTCTAAGTAC ACCCGGGTTT GGATTCCTGA CCCGGATGAG 
    GTCTGGAGGT CTGCTGAAAT CATCAAGGAT TATAAGGAGG GAGACACAGT GCTGCATCTC
    AAATTAGAAG ATGAATCGAC GTTAGAATAT CCAATTGGTT CCAAAGACAA TCCGCTTCCA
    TTTCTCCGGA ATCCCGATAT CCTGGTAGGA GAGAATGACC TCACGGCTCT CAGTTACCTC
    CATGAGCCTG CTGTGCTCCA CAACCTCAAG GTCCGCTTCC TCGAGTCCAA CCACATCTAC
    ACCTATTGTG GGATTGTTCT GGTGGCCATC AACCCGTACG AGCAGCTACA TATCTATGGA
    GAAGAAGTCA TCAATGCATA CAGCGGGCAG AACATGGGGG ACATGGACCC TCATATATTT
    GCGGTGGCCG AGGAAGCTTT TAAACAGATG GCCAGAGATG AGAAGAACCA GTCCATAATT
    GTGAGCGGTG AGTCGGGAGC TGGTAAAACC GTTTCGGCAA AGTATGCCAT GCGCTTCTTC
    GCCACAGTGG GAGGATCAGC AAATGACACC AATGTTGAAG AGAAAGTTTT GGCTTCCAGT
    CCCATTATGG AGGCCATTGG AAATGCAAAA ACAACCCGTA ATGACAACAG CAGCCGTTTT
    GGGAAATACA TCCAGATCGG CTTTGACAGG AGATATCATA TTATTGGTGC CAATATGAGA
    ACGTACCTGC TTGAGAAGTC CAGAGTAGTG TTTCAGGCTG AAGAGGAGAG GAACTACCAC
    ATCTTCTACC AGCTCTGTGC TTCATGCTCT CTGCCAGAGT TTAAGGATCT TACCTTGACC
    AGTGCAGAAG ACTTCACTTA CACGTCTCTC GGTGAGAACA TTTTCATTGA GGGAGTGAAT
    GATGCCGAGG ACCTTGTGAA GACGAGAGAA GCTCTTACGA TGCTTGGTGT AAAAGAAAAC
    CACCAAATGA GCATCTTTAA GATAATTGCC TCCATTTTGC ACCTGGGGAA CGTGGAGATT
    GTGCAGGAGA GAGATGGAGA ATCCTGCCAT ATCAATAGGA ACGACACTCA TCTGCATCAT
    TTCTGTCGGC TGTTGGGGAT TGAGCAGGAG CAGATGGAGA ACTGGCTATG CCGCAGGAAA
    CTGGTGACCA CTTCTGAAAC TTATGTGAAA AACATGCCCC ACAGCCAGGC AGTCAATGCC
    CGAGATGCCT TGGCCAAACA CATCTATGCC CATCTGTTTG ACTGGATCGT GGAGCACATC
    AACAAGTCCC TGCATACTTC CACTAAACAG CACTCATTCA TCGGGGTTCT GGATATCTAT
    GGGTTTGAAA CATTTGAGAT CAACAGCTTT GAGCAGTTCT GTATTAACTA CGCCAATGAG
    AAGCTACAGC AGCAATTCAA CTCGCATGTG TTTAAGCTGG AGCAAGAAGA GTACATGAAG
    GAGCAGATCC CCTGGACACT CATTGATTTC TATGATAACC AGCCGTGCAT TGACCTCATT
    GAAGCCAAAC TCGGTATCCT GGACCTTCTT GATGAAGAGT GTAAGGTGCC CAAAGGAACA
    GATCAGAACT GGGCGCAAAA GCTGTACAGC AAGCATTCAG GCAGCGGCCA TTTCGAAAAG
    CCCAGGATGT CCAACAAGTC CTTTATTGTT GTTCATTTTG CAGATAAGGT TGAATATCAG
    TGTGACGGAT TCCTGGAGAA GAACAGAGAC ACTGTTTATG AAGAGCAGAT AAACATCCTC
    AAGGCCAGTA AGTTCCAGTT GGTTGCAGAT CTCTTTGACG ATGCTAAAGA TGCTGCCCCT
    CATCCAGGCA GCAAAACCTC CAGAATCAAT GTTCGGCCTG CCAAACCCGC TCCGAAAGGT
    CACAACCGGG AGCACAGGAA AACTGTGGGC ACTCAGTTCA GAAACTCTCT GCATCTTCTG
    ATGGAGACAC TCAACGCAAC CACCCCTCAT TATGTTCGCT GCATTAAGCC CAATGATTAT
    AAGGAATCCT TTGTGTTTGA TTCTAGAAGA GCTGTTCAGC AACTAAGAGC ATGTGGTGTT
    CTGGAGACCA TTCGCATTAG CGCCGCCGGC TATCCATCAC GATGGACATA TCCAGATTTC
    TTCAGCAGAT ATCGAGTGCT CATGACAAAG AAGGATCTGT CAGTCGGAGA CAAGAAGCAG
    GTCTGTAAGA ACCTGCTGGA GATCTTGATC AAGGATCCAG ATAAGTTCCA GTTTGGAAAG
    ACTAAGATCT TCTTCAGGGC GGGTCAGGTG GCCTATCTGG AGAAGCTCAG AGCCGATAAG
    TTCCGCTTCG CCTGTATTAA GATCCAGAAG ACTGTGCGAG GATGGCTGCA GAGGATTCGG
    TATCGCAAGA TTCGCAAGTC TGCCATCACT TTACAAAGAT ACGGCCGGGG TTACTTGGCA
    CGCAGGTACG CAGAGATGCT TCGCCTGACC CGAGCAGCAG TCATTTGTCA GAAGCAGTAC
    CGCATGGTAC AGGTTCGCCG GGAGTATCTG AGGGTCCGTC GGGCTGTAAT CACCATTCAA
    GCATTCACCC GAGGCATGTT CATACGCAGG TTATATCAGG AGTTCCTGCT CCATCACAAA
    GCCATGATCA TACAGAAGAC TGTCCGTGGT TGGCTTGTAA GAAAGAAGTA TCAGCGTTCC
    CGATACGCTG CCATCGTCAT TCAGTGTTTC TATCGCCGCG TACGTGCCAA GCGGCAGCTC
    AAACAGCTCA AGATAGAGGC CCGTTCAGCC GAACACTTTA AAAAACTCAA TGTCGGAATG
    GAGAACAAGA TCGTGCAGCT CCAGAAGAAG ATGGACAACC AGTCAAAAGA GTTAAAATCT
    CAGAACGAAA ATCTGGCTGT TGCTAAAACT GTTCTGGAGA CAGAGGTTAG TAAGCTGAGC
    AAAGAGCTGG AGACCCTCCG CACTCGACAG GTGGCTGGCA CTCAGATGAC ATCACTGCAG
    GAAGAGCTGG AGAAACTGAG GGCGGAGCTT CAGGAAGCAC ATGCCCACAA GAAACTTTTG
    GAGGAGGAGT TCAGCAACGA GAAGCAAGGA CTCGAACAGA GGGTGGAGGA GCTTGAGAAA
    GAAAACACAG TCTTGAAGAA GGAAAAAGAA GAGATGAACC GCAGAATCCA AACCTCTACT
    CAGGATCAGG GAGGTGATGT GTCTCAGAAG GAGAGTCGCC TGCAGCATGA ACTGGATGAC
    GAGAGACAAC GATATCAGAA CCTGGTGAAA GAATATTCCC GACTGGAGCA GAGATACGAA
    AACCTGCAGG AGGACATGTC TTCAATGAAG TTTCATCCTG GGCATCGGAG AAACCCATCT
    AACCAGAGCA GCTTGGGTTC AGACTCCAAC TATCCCTCAA TTAGCTTGTC AGAGGTGGGG
    GACACTGATG ACACCATCCA GCAGGTGGAG GAGATGGGCA TAGAGAAGGC TGCCATGGAC
    CTCAGCGTTT TCATGAAGTT ACAGAAACGT GTACGAGAGC TGGAGCAGGA GAGGAAGCGG
    CTGCAGACCA ATCTAGATAA AGTAGAGGAA CTGAGCAAAC GCAAGGGAAG CGAGGCCAAG
    AACTCAACGT CAGAGGACGA TATAAACGCA GACCTGGCCT ACAACAGTCT GAAGCGGCAA
    GAGCTCGAAA CAGAGAATAA GAAGTTGAAG AATGACCTTA ATGAGTTAAG GAAAGCAGTG
    GCCGACAGGG CCTCTGAGAA CAACTCATCC AATGAGCTTC AAGATGGCTA CAACCTGCTG
    CTCAGTCAGC TCAAAGCGGC CAATGAGGAG CTGGATGTGC GCAAGGAGGA GGTCCTGATG
    CTCAAGACCC AGATCGTCAA CTCAACAAGA CAGACAGAAA ACTTAAACCA CATCAATTCC
    AACGATGTGT CTGAGTGGAC CGACAACAAG AGCCCTGTGA ACACGGAGGA CCGCGTGGAT
    GCGCCTGCAT CAAAGAATAA AGCTCAAGAC TGGGGTTATT TGAATGAAGA TGGCGAGCTA
    GGCCTGGCCT ACCAGGGTCT AAAGCAGGTT GCCAGACTGC TGGAGACCCA ACTGCAGACC
    CAGACTCGAC AGCACAGGGA TGAGTTGGAA GCCCTTCACA CACAGATCGA GCTCCTGAAG
    GACAATCTGG AGAAGAAGCA GGACATGCTG GACCACTTCT CCACACTTTC TCCTGATGCT
    CTGGTGGAGT TCAGCGTGCA ACAGGAGATC ACACGCCTCA CCAATGAAAA CCTGGACCTA
    AAGGAACTCG TTGAGAAGTT GGAAAAGAAT GAGAAGAAGC TGAAGAAGCA GTTGAGGATC
    TATATGAAGA AAGTGCAAGA GCTGGAAGTG ACTCAGGCCA CTAAAAGCAG ACCGGAGCTC
    ACTCGTCAGT TCACTGTCCA GAGAAGAGAG AAGGACTTTG AAGGCATGCT GGAGTACTAT
    AAAGAAGATG AGGCCTTGCT GGTCAAGACT TTAGTTACAG ACATGAAGCC CAACGCTGTG
    TCCGCCACTG TCCCCTGCCT CCCCGCATAC ATCCTCTTCA TGTGCATCCG TCACGCCGAC
    TACATCAATG ATGACCAGAA AGTTCATTCA CTACTGACCG CTACCATCAA CGCCATCAAA
    AAAGTGCTGA AGAAAAACAA CGATGACTTT GAGATGACCT CCTTTTGGCT GGCAAATGCA
    AGTCGACTTT TACACTGTCT GAAGCAGTAC AGCGGCGATG AGGCGTTCAT GACCCAGAAC
    TCTGCCAAGC AGAACGAGCA CTGCCTGAAG AACTTTGACC TGGCGGAGTA CCGACAGGTT
    CTGAGTGACC TCTCCATCCA AATCTATCAG CAGCTTGTGA AAGTTGCCGA GGCCAACATG
    CAGCCCATGA TCGTGTCTGC CATGCTTGAG AGCGAGAGCA TCCCCAGCCT GGCCGGAGTG
    AAGCCCATGG GTTACAGGAA CCGCTCCTCT AGTGTGGACT GTGAGAGTGG AGGTCCCGCA
    GGATACACGC TTCAGGCACT GATCAAACAG CTGGCTCAGT TTTACTCTAC CATGGCGGAC
    CACGGCCTGG ACCCTGAGAT CAGCCAGCAG GTGCTTCGCC AGCTCTTCTA CTCCATCAAC
    GCAGTGACCC TCAACAACCT GCTGCTGCGC AAGGACGTCT GCTCCTGGAG CACCGGCATG
    CAGCTCAGGT ACAACATCAG TCAGCTGGAG GAGTGGCTAA GAGGGAAGAA CCTGCACCAG
    TGTGGAGCTG TGGCCACTAT GGAGCCGGTC ATTCAGGCCG CGCAGCTGCT GCAGGTGAAG
    AAGAAGACAT CACAGGACGC TGAAGCCATC TGCTCGCTCT GCACTGCACT CAGTTTACAG
    CAGATTGTGA AGATTCTGAA CTTGTACACA CCACTCAATG AGTTTGAGGA GCGAGTCACT
    GTTTCCTTCA TAAGAGACAT ACAGAATCGC CTCCAAGATC GAATAGAAAA CAACCAACTG
    CTGGCGGACA CGAAATACAC CTTCCCCGTG CTCTTCCCGT ACACTCCTTC CAACCTCAGC
    CTGGAAACCA TCCACATCCC AGCCTCCCTC AGCCTGGACT TCCTGACGCG GGAGAACTTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150933
    Clone ID Related Accession (Same CDS sequence) XM_021468887.1
    Accession Version XM_021468887.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product unconventional myosin-Vb isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGTGAAC ATGAGCTTTA TGTCAAGTAC ACCCGGGTTT GGATTCCTGA CCCGGATGAG 
    GTCTGGAGGT CTGCTGAAAT CATCAAGGAT TATAAGGAGG GAGACACAGT GCTGCATCTC
    AAATTAGAAG ATGAATCGAC GTTAGAATAT CCAATTGGTT CCAAAGACAA TCCGCTTCCA
    TTTCTCCGGA ATCCCGATAT CCTGGTAGGA GAGAATGACC TCACGGCTCT CAGTTACCTC
    CATGAGCCTG CTGTGCTCCA CAACCTCAAG GTCCGCTTCC TCGAGTCCAA CCACATCTAC
    ACCTATTGTG GGATTGTTCT GGTGGCCATC AACCCGTACG AGCAGCTACA TATCTATGGA
    GAAGAAGTCA TCAATGCATA CAGCGGGCAG AACATGGGGG ACATGGACCC TCATATATTT
    GCGGTGGCCG AGGAAGCTTT TAAACAGATG GCCAGAGATG AGAAGAACCA GTCCATAATT
    GTGAGCGGTG AGTCGGGAGC TGGTAAAACC GTTTCGGCAA AGTATGCCAT GCGCTTCTTC
    GCCACAGTGG GAGGATCAGC AAATGACACC AATGTTGAAG AGAAAGTTTT GGCTTCCAGT
    CCCATTATGG AGGCCATTGG AAATGCAAAA ACAACCCGTA ATGACAACAG CAGCCGTTTT
    GGGAAATACA TCCAGATCGG CTTTGACAGG AGATATCATA TTATTGGTGC CAATATGAGA
    ACGTACCTGC TTGAGAAGTC CAGAGTAGTG TTTCAGGCTG AAGAGGAGAG GAACTACCAC
    ATCTTCTACC AGCTCTGTGC TTCATGCTCT CTGCCAGAGT TTAAGGATCT TACCTTGACC
    AGTGCAGAAG ACTTCACTTA CACGTCTCTC GGTGAGAACA TTTTCATTGA GGGAGTGAAT
    GATGCCGAGG ACCTTGTGAA GACGAGAGAA GCTCTTACGA TGCTTGGTGT AAAAGAAAAC
    CACCAAATGA GCATCTTTAA GATAATTGCC TCCATTTTGC ACCTGGGGAA CGTGGAGATT
    GTGCAGGAGA GAGATGGAGA ATCCTGCCAT ATCAATAGGA ACGACACTCA TCTGCATCAT
    TTCTGTCGGC TGTTGGGGAT TGAGCAGGAG CAGATGGAGA ACTGGCTATG CCGCAGGAAA
    CTGGTGACCA CTTCTGAAAC TTATGTGAAA AACATGCCCC ACAGCCAGGC AGTCAATGCC
    CGAGATGCCT TGGCCAAACA CATCTATGCC CATCTGTTTG ACTGGATCGT GGAGCACATC
    AACAAGTCCC TGCATACTTC CACTAAACAG CACTCATTCA TCGGGGTTCT GGATATCTAT
    GGGTTTGAAA CATTTGAGAT CAACAGCTTT GAGCAGTTCT GTATTAACTA CGCCAATGAG
    AAGCTACAGC AGCAATTCAA CTCGCATGTG TTTAAGCTGG AGCAAGAAGA GTACATGAAG
    GAGCAGATCC CCTGGACACT CATTGATTTC TATGATAACC AGCCGTGCAT TGACCTCATT
    GAAGCCAAAC TCGGTATCCT GGACCTTCTT GATGAAGAGT GTAAGGTGCC CAAAGGAACA
    GATCAGAACT GGGCGCAAAA GCTGTACAGC AAGCATTCAG GCAGCGGCCA TTTCGAAAAG
    CCCAGGATGT CCAACAAGTC CTTTATTGTT GTTCATTTTG CAGATAAGGT TGAATATCAG
    TGTGACGGAT TCCTGGAGAA GAACAGAGAC ACTGTTTATG AAGAGCAGAT AAACATCCTC
    AAGGCCAGTA AGTTCCAGTT GGTTGCAGAT CTCTTTGACG ATGCTAAAGA TGCTGCCCCT
    CATCCAGGCA GCAAAACCTC CAGAATCAAT GTTCGGCCTG CCAAACCCGC TCCGAAAGGT
    CACAACCGGG AGCACAGGAA AACTGTGGGC ACTCAGTTCA GAAACTCTCT GCATCTTCTG
    ATGGAGACAC TCAACGCAAC CACCCCTCAT TATGTTCGCT GCATTAAGCC CAATGATTAT
    AAGGAATCCT TTGTGTTTGA TTCTAGAAGA GCTGTTCAGC AACTAAGAGC ATGTGGTGTT
    CTGGAGACCA TTCGCATTAG CGCCGCCGGC TATCCATCAC GATGGACATA TCCAGATTTC
    TTCAGCAGAT ATCGAGTGCT CATGACAAAG AAGGATCTGT CAGTCGGAGA CAAGAAGCAG
    GTCTGTAAGA ACCTGCTGGA GATCTTGATC AAGGATCCAG ATAAGTTCCA GTTTGGAAAG
    ACTAAGATCT TCTTCAGGGC GGGTCAGGTG GCCTATCTGG AGAAGCTCAG AGCCGATAAG
    TTCCGCTTCG CCTGTATTAA GATCCAGAAG ACTGTGCGAG GATGGCTGCA GAGGATTCGG
    TATCGCAAGA TTCGCAAGTC TGCCATCACT TTACAAAGAT ACGGCCGGGG TTACTTGGCA
    CGCAGGTACG CAGAGATGCT TCGCCTGACC CGAGCAGCAG TCATTTGTCA GAAGCAGTAC
    CGCATGGTAC AGGTTCGCCG GGAGTATCTG AGGGTCCGTC GGGCTGTAAT CACCATTCAA
    GCATTCACCC GAGGCATGTT CATACGCAGG TTATATCAGG AGTTCCTGCT CCATCACAAA
    GCCATGATCA TACAGAAGAC TGTCCGTGGT TGGCTTGTAA GAAAGAAGTA TCAGCGTTCC
    CGATACGCTG CCATCGTCAT TCAGTGTTTC TATCGCCGCG TACGTGCCAA GCGGCAGCTC
    AAACAGCTCA AGATAGAGGC CCGTTCAGCC GAACACTTTA AAAAACTCAA TGTCGGAATG
    GAGAACAAGA TCGTGCAGCT CCAGAAGAAG ATGGACAACC AGTCAAAAGA GTTAAAATCT
    CAGAACGAAA ATCTGGCTGT TGCTAAAACT GTTCTGGAGA CAGAGGTTAG TAAGCTGAGC
    AAAGAGCTGG AGACCCTCCG CACTCGACAG GTGGCTGGCA CTCAGATGAC ATCACTGCAG
    GAAGAGCTGG AGAAACTGAG GGCGGAGCTT CAGGAAGCAC ATGCCCACAA GAAACTTTTG
    GAGGAGGAGT TCAGCAACGA GAAGCAAGGA CTCGAACAGA GGGTGGAGGA GCTTGAGAAA
    GAAAACACAG TCTTGAAGAA GGAAAAAGAA GAGATGAACC GCAGAATCCA AACCTCTACT
    CAGGATCAGG GAGGTGATGT GTCTCAGAAG GAGAGTCGCC TGCAGCATGA ACTGGATGAC
    GAGAGACAAC GATATCAGAA CCTGGTGAAA GAATATTCCC GACTGGAGCA GAGATACGAA
    AACCTGCAGG AGGACATGTC TTCAATGAAG TTTCATCCTG GGCATCGGAG AAACCCATCT
    AACCAGAGCA GCTTGGGTTC AGACTCCAAC TATCCCTCAA TTAGCTTGTC AGAGGTGGGG
    GACACTGATG ACACCATCCA GCAGGTGGAG GAGATGGGCA TAGAGAAGGC TGCCATGGAC
    CTCAGCGTTT TCATGAAGTT ACAGAAACGT GTACGAGAGC TGGAGCAGGA GAGGAAGCGG
    CTGCAGACCA ATCTAGATAA AGTAGAGGAA CTGAGCAAAC GCAAGGGAAG CGAGGCCAAG
    AACTCAACGT CAGAGGACGA TATAAACGCA GACCTGGCCT ACAACAGTCT GAAGCGGCAA
    GAGCTCGAAA CAGAGAATAA GAAGTTGAAG AATGACCTTA ATGAGTTAAG GAAAGCAGTG
    GCCGACAGGG CCTCTGAGAA CAACTCATCC AATGAGCTTC AAGATGGCTA CAACCTGCTG
    CTCAGTCAGC TCAAAGCGGC CAATGAGGAG CTGGATGTGC GCAAGGAGGA GGTCCTGATG
    CTCAAGACCC AGATCGTCAA CTCAACAAGA CAGACAGAAA ACTTAAACCA CATCAATTCC
    AACGATGTGT CTGAGTGGAC CGACAACAAG AGCCCTGTGA ACACGGAGGA CCGCGTGGAT
    GCGCCTGCAT CAAAGAATAA AGCTCAAGAC TGGGGTTATT TGAATGAAGA TGGCGAGCTA
    GGCCTGGCCT ACCAGGGTCT AAAGCAGGTT GCCAGACTGC TGGAGACCCA ACTGCAGACC
    CAGACTCGAC AGCACAGGGA TGAGTTGGAA GCCCTTCACA CACAGATCGA GCTCCTGAAG
    GACAATCTGG AGAAGAAGCA GGACATGCTG GACCACTTCT CCACACTTTC TCCTGATGCT
    CTGGTGGAGT TCAGCGTGCA ACAGGAGATC ACACGCCTCA CCAATGAAAA CCTGGACCTA
    AAGGAACTCG TTGAGAAGTT GGAAAAGAAT GAGAAGAAGC TGAAGAAGCA GTTGAGGATC
    TATATGAAGA AAGTGCAAGA GCTGGAAGTG ACTCAGGCCA CTAAAAGCAG ACCGGAGCTC
    ACTCGTCAGT TCACTGTCCA GAGAAGAGAG AAGGACTTTG AAGGCATGCT GGAGTACTAT
    AAAGAAGATG AGGCCTTGCT GGTCAAGACT TTAGTTACAG ACATGAAGCC CAACGCTGTG
    TCCGCCACTG TCCCCTGCCT CCCCGCATAC ATCCTCTTCA TGTGCATCCG TCACGCCGAC
    TACATCAATG ATGACCAGAA AGTTCATTCA CTACTGACCG CTACCATCAA CGCCATCAAA
    AAAGTGCTGA AGAAAAACAA CGATGACTTT GAGATGACCT CCTTTTGGCT GGCAAATGCA
    AGTCGACTTT TACACTGTCT GAAGCAGTAC AGCGGCGATG AGGCGTTCAT GACCCAGAAC
    TCTGCCAAGC AGAACGAGCA CTGCCTGAAG AACTTTGACC TGGCGGAGTA CCGACAGGTT
    CTGAGTGACC TCTCCATCCA AATCTATCAG CAGCTTGTGA AAGTTGCCGA GGCCAACATG
    CAGCCCATGA TCGTGTCTGC CATGCTTGAG AGCGAGAGCA TCCCCAGCCT GGCCGGAGTG
    AAGCCCATGG GTTACAGGAA CCGCTCCTCT AGTGTGGACT GTGAGAGTGG AGGTCCCGCA
    GGATACACGC TTCAGGCACT GATCAAACAG CTGGCTCAGT TTTACTCTAC CATGGCGGAC
    CACGGCCTGG ACCCTGAGAT CAGCCAGCAG GTGCTTCGCC AGCTCTTCTA CTCCATCAAC
    GCAGTGACCC TCAACAACCT GCTGCTGCGC AAGGACGTCT GCTCCTGGAG CACCGGCATG
    CAGCTCAGGT ACAACATCAG TCAGCTGGAG GAGTGGCTAA GAGGGAAGAA CCTGCACCAG
    TGTGGAGCTG TGGCCACTAT GGAGCCGGTC ATTCAGGCCG CGCAGCTGCT GCAGGTGAAG
    AAGAAGACAT CACAGGACGC TGAAGCCATC TGCTCGCTCT GCACTGCACT CAGTTTACAG
    CAGATTGTGA AGATTCTGAA CTTGTACACA CCACTCAATG AGTTTGAGGA GCGAGTCACT
    GTTTCCTTCA TAAGAGACAT ACAGAATCGC CTCCAAGATC GAATAGAAAA CAACCAACTG
    CTGGCGGACA CGAAATACAC CTTCCCCGTG CTCTTCCCGT ACACTCCTTC CAACCTCAGC
    CTGGAAACCA TCCACATCCC AGCCTCCCTC AGCCTGGACT TCCTGACGCG GGAGAACTTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324562.1
    CDS157..5679
    Translation

    Target ORF information:

    RefSeq Version XM_021468887.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio myosin VB (myo5b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468887.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGTGAAC ATGAGCTTTA TGTCAAGTAC ACCCGGGTTT GGATTCCTGA CCCGGATGAG 
    GTCTGGAGGT CTGCTGAAAT CATCAAGGAT TATAAGGAGG GAGACACAGT GCTGCATCTC
    AAATTAGAAG ATGAATCGAC GTTAGAATAT CCAATTGGTT CCAAAGACAA TCCGCTTCCA
    TTTCTCCGGA ATCCCGATAT CCTGGTAGGA GAGAATGACC TCACGGCTCT CAGTTACCTC
    CATGAGCCTG CTGTGCTCCA CAACCTCAAG GTCCGCTTCC TCGAGTCCAA CCACATCTAC
    ACCTATTGTG GGATTGTTCT GGTGGCCATC AACCCGTACG AGCAGCTACA TATCTATGGA
    GAAGAAGTCA TCAATGCATA CAGCGGGCAG AACATGGGGG ACATGGACCC TCATATATTT
    GCGGTGGCCG AGGAAGCTTT TAAACAGATG GCCAGAGATG AGAAGAACCA GTCCATAATT
    GTGAGCGGTG AGTCGGGAGC TGGTAAAACC GTTTCGGCAA AGTATGCCAT GCGCTTCTTC
    GCCACAGTGG GAGGATCAGC AAATGACACC AATGTTGAAG AGAAAGTTTT GGCTTCCAGT
    CCCATTATGG AGGCCATTGG AAATGCAAAA ACAACCCGTA ATGACAACAG CAGCCGTTTT
    GGGAAATACA TCCAGATCGG CTTTGACAGG AGATATCATA TTATTGGTGC CAATATGAGA
    ACGTACCTGC TTGAGAAGTC CAGAGTAGTG TTTCAGGCTG AAGAGGAGAG GAACTACCAC
    ATCTTCTACC AGCTCTGTGC TTCATGCTCT CTGCCAGAGT TTAAGGATCT TACCTTGACC
    AGTGCAGAAG ACTTCACTTA CACGTCTCTC GGTGAGAACA TTTTCATTGA GGGAGTGAAT
    GATGCCGAGG ACCTTGTGAA GACGAGAGAA GCTCTTACGA TGCTTGGTGT AAAAGAAAAC
    CACCAAATGA GCATCTTTAA GATAATTGCC TCCATTTTGC ACCTGGGGAA CGTGGAGATT
    GTGCAGGAGA GAGATGGAGA ATCCTGCCAT ATCAATAGGA ACGACACTCA TCTGCATCAT
    TTCTGTCGGC TGTTGGGGAT TGAGCAGGAG CAGATGGAGA ACTGGCTATG CCGCAGGAAA
    CTGGTGACCA CTTCTGAAAC TTATGTGAAA AACATGCCCC ACAGCCAGGC AGTCAATGCC
    CGAGATGCCT TGGCCAAACA CATCTATGCC CATCTGTTTG ACTGGATCGT GGAGCACATC
    AACAAGTCCC TGCATACTTC CACTAAACAG CACTCATTCA TCGGGGTTCT GGATATCTAT
    GGGTTTGAAA CATTTGAGAT CAACAGCTTT GAGCAGTTCT GTATTAACTA CGCCAATGAG
    AAGCTACAGC AGCAATTCAA CTCGCATGTG TTTAAGCTGG AGCAAGAAGA GTACATGAAG
    GAGCAGATCC CCTGGACACT CATTGATTTC TATGATAACC AGCCGTGCAT TGACCTCATT
    GAAGCCAAAC TCGGTATCCT GGACCTTCTT GATGAAGAGT GTAAGGTGCC CAAAGGAACA
    GATCAGAACT GGGCGCAAAA GCTGTACAGC AAGCATTCAG GCAGCGGCCA TTTCGAAAAG
    CCCAGGATGT CCAACAAGTC CTTTATTGTT GTTCATTTTG CAGATAAGGT TGAATATCAG
    TGTGACGGAT TCCTGGAGAA GAACAGAGAC ACTGTTTATG AAGAGCAGAT AAACATCCTC
    AAGGCCAGTA AGTTCCAGTT GGTTGCAGAT CTCTTTGACG ATGCTAAAGA TGCTGCCCCT
    CATCCAGGCA GCAAAACCTC CAGAATCAAT GTTCGGCCTG CCAAACCCGC TCCGAAAGGT
    CACAACCGGG AGCACAGGAA AACTGTGGGC ACTCAGTTCA GAAACTCTCT GCATCTTCTG
    ATGGAGACAC TCAACGCAAC CACCCCTCAT TATGTTCGCT GCATTAAGCC CAATGATTAT
    AAGGAATCCT TTGTGTTTGA TTCTAGAAGA GCTGTTCAGC AACTAAGAGC ATGTGGTGTT
    CTGGAGACCA TTCGCATTAG CGCCGCCGGC TATCCATCAC GATGGACATA TCCAGATTTC
    TTCAGCAGAT ATCGAGTGCT CATGACAAAG AAGGATCTGT CAGTCGGAGA CAAGAAGCAG
    GTCTGTAAGA ACCTGCTGGA GATCTTGATC AAGGATCCAG ATAAGTTCCA GTTTGGAAAG
    ACTAAGATCT TCTTCAGGGC GGGTCAGGTG GCCTATCTGG AGAAGCTCAG AGCCGATAAG
    TTCCGCTTCG CCTGTATTAA GATCCAGAAG ACTGTGCGAG GATGGCTGCA GAGGATTCGG
    TATCGCAAGA TTCGCAAGTC TGCCATCACT TTACAAAGAT ACGGCCGGGG TTACTTGGCA
    CGCAGGTACG CAGAGATGCT TCGCCTGACC CGAGCAGCAG TCATTTGTCA GAAGCAGTAC
    CGCATGGTAC AGGTTCGCCG GGAGTATCTG AGGGTCCGTC GGGCTGTAAT CACCATTCAA
    GCATTCACCC GAGGCATGTT CATACGCAGG TTATATCAGG AGTTCCTGCT CCATCACAAA
    GCCATGATCA TACAGAAGAC TGTCCGTGGT TGGCTTGTAA GAAAGAAGTA TCAGCGTTCC
    CGATACGCTG CCATCGTCAT TCAGTGTTTC TATCGCCGCG TACGTGCCAA GCGGCAGCTC
    AAACAGCTCA AGATAGAGGC CCGTTCAGCC GAACACTTTA AAAAACTCAA TGTCGGAATG
    GAGAACAAGA TCGTGCAGCT CCAGAAGAAG ATGGACAACC AGTCAAAAGA GTTAAAATCT
    CAGAACGAAA ATCTGGCTGT TGCTAAAACT GTTCTGGAGA CAGAGGTTAG TAAGCTGAGC
    AAAGAGCTGG AGACCCTCCG CACTCGACAG GTGGCTGGCA CTCAGATGAC ATCACTGCAG
    GAAGAGCTGG AGAAACTGAG GGCGGAGCTT CAGGAAGCAC ATGCCCACAA GAAACTTTTG
    GAGGAGGAGT TCAGCAACGA GAAGCAAGGA CTCGAACAGA GGGTGGAGGA GCTTGAGAAA
    GAAAACACAG TCTTGAAGAA GGAAAAAGAA GAGATGAACC GCAGAATCCA AACCTCTACT
    CAGGATCAGG GAGGTGATGT GTCTCAGAAG GAGAGTCGCC TGCAGCATGA ACTGGATGAC
    GAGAGACAAC GATATCAGAA CCTGGTGAAA GAATATTCCC GACTGGAGCA GAGATACGAA
    AACCTGCAGG AGGACATGTC TTCAATGAAG TTTCATCCTG GGCATCGGAG AAACCCATCT
    AACCAGAGCA GCTTGGGTTC AGACTCCAAC TATCCCTCAA TTAGCTTGTC AGAGGTGGGG
    GACACTGATG ACACCATCCA GCAGGTGGAG GAGATGGGCA TAGAGAAGGC TGCCATGGAC
    CTCAGCGTTT TCATGAAGTT ACAGAAACGT GTACGAGAGC TGGAGCAGGA GAGGAAGCGG
    CTGCAGACCA ATCTAGATAA AGTAGAGGAA CTGAGCAAAC GCAAGGGAAG CGAGGCCAAG
    AACTCAACGT CAGAGGACGA TATAAACGCA GACCTGGCCT ACAACAGTCT GAAGCGGCAA
    GAGCTCGAAA CAGAGAATAA GAAGTTGAAG AATGACCTTA ATGAGTTAAG GAAAGCAGTG
    GCCGACAGGG CCTCTGAGAA CAACTCATCC AATGAGCTTC AAGATGGCTA CAACCTGCTG
    CTCAGTCAGC TCAAAGCGGC CAATGAGGAG CTGGATGTGC GCAAGGAGGA GGTCCTGATG
    CTCAAGACCC AGATCGTCAA CTCAACAAGA CAGACAGAAA ACTTAAACCA CATCAATTCC
    AACGATGTGT CTGAGTGGAC CGACAACAAG AGCCCTGTGA ACACGGAGGA CCGCGTGGAT
    GCGCCTGCAT CAAAGAATAA AGCTCAAGAC TGGGGTTATT TGAATGAAGA TGGCGAGCTA
    GGCCTGGCCT ACCAGGGTCT AAAGCAGGTT GCCAGACTGC TGGAGACCCA ACTGCAGACC
    CAGACTCGAC AGCACAGGGA TGAGTTGGAA GCCCTTCACA CACAGATCGA GCTCCTGAAG
    GACAATCTGG AGAAGAAGCA GGACATGCTG GACCACTTCT CCACACTTTC TCCTGATGCT
    CTGGTGGAGT TCAGCGTGCA ACAGGAGATC ACACGCCTCA CCAATGAAAA CCTGGACCTA
    AAGGAACTCG TTGAGAAGTT GGAAAAGAAT GAGAAGAAGC TGAAGAAGCA GTTGAGGATC
    TATATGAAGA AAGTGCAAGA GCTGGAAGTG ACTCAGGCCA CTAAAAGCAG ACCGGAGCTC
    ACTCGTCAGT TCACTGTCCA GAGAAGAGAG AAGGACTTTG AAGGCATGCT GGAGTACTAT
    AAAGAAGATG AGGCCTTGCT GGTCAAGACT TTAGTTACAG ACATGAAGCC CAACGCTGTG
    TCCGCCACTG TCCCCTGCCT CCCCGCATAC ATCCTCTTCA TGTGCATCCG TCACGCCGAC
    TACATCAATG ATGACCAGAA AGTTCATTCA CTACTGACCG CTACCATCAA CGCCATCAAA
    AAAGTGCTGA AGAAAAACAA CGATGACTTT GAGATGACCT CCTTTTGGCT GGCAAATGCA
    AGTCGACTTT TACACTGTCT GAAGCAGTAC AGCGGCGATG AGGCGTTCAT GACCCAGAAC
    TCTGCCAAGC AGAACGAGCA CTGCCTGAAG AACTTTGACC TGGCGGAGTA CCGACAGGTT
    CTGAGTGACC TCTCCATCCA AATCTATCAG CAGCTTGTGA AAGTTGCCGA GGCCAACATG
    CAGCCCATGA TCGTGTCTGC CATGCTTGAG AGCGAGAGCA TCCCCAGCCT GGCCGGAGTG
    AAGCCCATGG GTTACAGGAA CCGCTCCTCT AGTGTGGACT GTGAGAGTGG AGGTCCCGCA
    GGATACACGC TTCAGGCACT GATCAAACAG CTGGCTCAGT TTTACTCTAC CATGGCGGAC
    CACGGCCTGG ACCCTGAGAT CAGCCAGCAG GTGCTTCGCC AGCTCTTCTA CTCCATCAAC
    GCAGTGACCC TCAACAACCT GCTGCTGCGC AAGGACGTCT GCTCCTGGAG CACCGGCATG
    CAGCTCAGGT ACAACATCAG TCAGCTGGAG GAGTGGCTAA GAGGGAAGAA CCTGCACCAG
    TGTGGAGCTG TGGCCACTAT GGAGCCGGTC ATTCAGGCCG CGCAGCTGCT GCAGGTGAAG
    AAGAAGACAT CACAGGACGC TGAAGCCATC TGCTCGCTCT GCACTGCACT CAGTTTACAG
    CAGATTGTGA AGATTCTGAA CTTGTACACA CCACTCAATG AGTTTGAGGA GCGAGTCACT
    GTTTCCTTCA TAAGAGACAT ACAGAATCGC CTCCAAGATC GAATAGAAAA CAACCAACTG
    CTGGCGGACA CGAAATACAC CTTCCCCGTG CTCTTCCCGT ACACTCCTTC CAACCTCAGC
    CTGGAAACCA TCCACATCCC AGCCTCCCTC AGCCTGGACT TCCTGACGCG GGAGAACTTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150934
    Clone ID Related Accession (Same CDS sequence) XM_021468888.1
    Accession Version XM_021468888.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5445bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product unconventional myosin-Vb isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCTGTAA CTGAACTCTA CTCTAAGTAC ACCCGGGTTT GGATTCCTGA CCCGGATGAG 
    GTCTGGAGGT CTGCTGAAAT CATCAAGGAT TATAAGGAGG GAGACACAGT GCTGCATCTC
    AAATTAGAAG ATGAATCGAC GTTAGAATAT CCAATTGGTT CCAAAGACAA TCCGCTTCCA
    TTTCTCCGGA ATCCCGATAT CCTGGTAGGA GAGAATGACC TCACGGCTCT CAGTTACCTC
    CATGAGCCTG CTGTGCTCCA CAACCTCAAG GTCCGCTTCC TCGAGTCCAA CCACATCTAC
    ACCTATTGTG GGATTGTTCT GGTGGCCATC AACCCGTACG AGCAGCTACA TATCTATGGA
    GAAGAAGTCA TCAATGCATA CAGCGGGCAG AACATGGGGG ACATGGACCC TCATATATTT
    GCGGTGGCCG AGGAAGCTTT TAAACAGATG GCCAGAGATG AGAAGAACCA GTCCATAATT
    GTGAGCGGTG AGTCGGGAGC TGGTAAAACC GTTTCGGCAA AGTATGCCAT GCGCTTCTTC
    GCCACAGTGG GAGGATCAGC AAATGACACC AATGTTGAAG AGAAAGTTTT GGCTTCCAGT
    CCCATTATGG AGGCCATTGG AAATGCAAAA ACAACCCGTA ATGACAACAG CAGCCGTTTT
    GGGAAATACA TCCAGATCGG CTTTGACAGG AGATATCATA TTATTGGTGC CAATATGAGA
    ACGTACCTGC TTGAGAAGTC CAGAGTAGTG TTTCAGGCTG AAGAGGAGAG GAACTACCAC
    ATCTTCTACC AGCTCTGTGC TTCATGCTCT CTGCCAGAGT TTAAGGATCT TACCTTGACC
    AGTGCAGAAG ACTTCACTTA CACGTCTCTC GGTGAGAACA TTTTCATTGA GGGAGTGAAT
    GATGCCGAGG ACCTTGTGAA GACGAGAGAA GCTCTTACGA TGCTTGGTGT AAAAGAAAAC
    CACCAAATGA GCATCTTTAA GATAATTGCC TCCATTTTGC ACCTGGGGAA CGTGGAGATT
    GTGCAGGAGA GAGATGGAGA ATCCTGCCAT ATCAATAGGA ACGACACTCA TCTGCATCAT
    TTCTGTCGGC TGTTGGGGAT TGAGCAGGAG CAGATGGAGA ACTGGCTATG CCGCAGGAAA
    CTGGTGACCA CTTCTGAAAC TTATGTGAAA AACATGCCCC ACAGCCAGGC AGTCAATGCC
    CGAGATGCCT TGGCCAAACA CATCTATGCC CATCTGTTTG ACTGGATCGT GGAGCACATC
    AACAAGTCCC TGCATACTTC CACTAAACAG CACTCATTCA TCGGGGTTCT GGATATCTAT
    GGGTTTGAAA CATTTGAGAT CAACAGCTTT GAGCAGTTCT GTATTAACTA CGCCAATGAG
    AAGCTACAGC AGCAATTCAA CTCGCATGTG TTTAAGCTGG AGCAAGAAGA GTACATGAAG
    GAGCAGATCC CCTGGACACT CATTGATTTC TATGATAACC AGCCGTGCAT TGACCTCATT
    GAAGCCAAAC TCGGTATCCT GGACCTTCTT GATGAAGAGT GTAAGGTGCC CAAAGGAACA
    GATCAGAACT GGGCGCAAAA GCTGTACAGC AAGCATTCAG GCAGCGGCCA TTTCGAAAAG
    CCCAGGATGT CCAACAAGTC CTTTATTGTT GTTCATTTTG CAGATAAGGT TGAATATCAG
    TGTGACGGAT TCCTGGAGAA GAACAGAGAC ACTGTTTATG AAGAGCAGAT AAACATCCTC
    AAGGCCAGTA AGTTCCAGTT GGTTGCAGAT CTCTTTGACG ATGCTAAAGA TGCTGCCCCT
    CATCCAGGCA GCAAAACCTC CAGAATCAAT GTTCGGCCTG CCAAACCCGC TCCGAAAGGT
    CACAACCGGG AGCACAGGAA AACTGTGGGC ACTCAGTTCA GAAACTCTCT GCATCTTCTG
    ATGGAGACAC TCAACGCAAC CACCCCTCAT TATGTTCGCT GCATTAAGCC CAATGATTAT
    AAGGAATCCT TTGTGTTTGA TTCTAGAAGA GCTGTTCAGC AACTAAGAGC ATGTGGTGTT
    CTGGAGACCA TTCGCATTAG CGCCGCCGGC TATCCATCAC GATGGACATA TCCAGATTTC
    TTCAGCAGAT ATCGAGTGCT CATGACAAAG AAGGATCTGT CAGTCGGAGA CAAGAAGCAG
    GTCTGTAAGA ACCTGCTGGA GATCTTGATC AAGGATCCAG ATAAGTTCCA GTTTGGAAAG
    ACTAAGATCT TCTTCAGGGC GGGTCAGGTG GCCTATCTGG AGAAGCTCAG AGCCGATAAG
    TTCCGCTTCG CCTGTATTAA GATCCAGAAG ACTGTGCGAG GATGGCTGCA GAGGATTCGG
    TATCGCAAGA TTCGCAAGTC TGCCATCACT TTACAAAGAT ACGGCCGGGG TTACTTGGCA
    CGCAGGTACG CAGAGATGCT TCGCCTGACC CGAGCAGCAG TCATTTGTCA GAAGCAGTAC
    CGCATGGTAC AGGTTCGCCG GGAGTATCTG AGGGTCCGTC GGGCTGTAAT CACCATTCAA
    GCATTCACCC GAGGCATGTT CATACGCAGG TTATATCAGG AGTTCCTGCT CCATCACAAA
    GCCATGATCA TACAGAAGAC TGTCCGTGGT TGGCTTGTAA GAAAGAAGTA TCAGCGTTCC
    CGATACGCTG CCATCGTCAT TCAGTGTTTC TATCGCCGCG TACGTGCCAA GCGGCAGCTC
    AAACAGCTCA AGATAGAGGC CCGTTCAGCC GAACACTTTA AAAAACTCAA TGTCGGAATG
    GAGAACAAGA TCGTGCAGCT CCAGAAGAAG ATGGACAACC AGTCAAAAGA GTTAAAATCT
    CAGAACGAAA ATCTGGCTGT TGCTAAAACT GTTCTGGAGA CAGAGGTTAG TAAGCTGAGC
    AAAGAGCTGG AGACCCTCCG CACTCGACAG GTGGCTGGCA CTCAGATGAC ATCACTGCAG
    GAAGAGCTGG AGAAACTGAG GGCGGAGCTT CAGGAAGCAC ATGCCCACAA GAAACTTTTG
    GAGGAGGAGT TCAGCAACGA GAAGCAAGGA CTCGAACAGA GGGTGGAGGA GCTTGAGAAA
    GAAAACACAG TCTTGAAGAA GGAAAAAGAA GAGATGAACC GCAGAATCCA AACCTCTACT
    CAGGATCAGG GAGGTGATGT GTCTCAGAAG GAGAGTCGCC TGCAGCATGA ACTGGATGAC
    GAGAGACAAC GATATCAGAA CCTGGTGAAA GAATATTCCC GACTGGAGCA GAGATACGAA
    AACCTGCAGG AGGACATGTC TTCAATGAAG TTTCATCCTG GGCATCGGAG AAACCCATCT
    AACCAGAGCA GCTTGGGTTC AGACTCCAAC TATCCCTCAA TTAGCTTGTC AGAGGTGGGG
    GACACTGATG ACACCATCCA GCAGGTGGAG GAGATGGGCA TAGAGAAGGC TGCCATGGAC
    CTCAGCGTTT TCATGAAGTT ACAGAAACGT GTACGAGAGC TGGAGCAGGA GAGGAAGCGG
    CTGCAGACCA ATCTAGATAA AGTAGAGGAA CTGAGCAAAC GCAAGGGAAG CGAGGCCAAG
    AACTCAACGT CAGAGGACGA TATAAACGCA GACCTGGCCT ACAACAGTCT GAAGCGGCAA
    GAGCTCGAAA CAGAGAATAA GAAGTTGAAG AATGACCTTA ATGAGTTAAG GAAAGCAGTG
    GCCGACAGGG CCTCTGAGAA CAACTCATCC AATGAGCTTC AAGATGGCTA CAACCTGCTG
    CTCAGTCAGC TCAAAGCGGC CAATGAGGAG CTGGATGTGC GCAAGGAGGA GGTCCTGATG
    CTCAAGACCC AGATCGTCAA CTCAACAAGA CAGACAGAAA ACTTAAACCA CATCAATTCC
    AACGATGTGT CTGAGTGGAC CGACAACAAG AGCCCTGTGA ACACGGAGGA CCGCGTGGAT
    GCGCCTGCAT CAAAGAAACT GCTGGAGACC CAACTGCAGA CCCAGACTCG ACAGCACAGG
    GATGAGTTGG AAGCCCTTCA CACACAGATC GAGCTCCTGA AGGACAATCT GGAGAAGAAG
    CAGGACATGC TGGACCACTT CTCCACACTT TCTCCTGATG CTCTGGTGGA GTTCAGCGTG
    CAACAGGAGA TCACACGCCT CACCAATGAA AACCTGGACC TAAAGGAACT CGTTGAGAAG
    TTGGAAAAGA ATGAGAAGAA GCTGAAGAAG CAGTTGAGGA TCTATATGAA GAAAGTGCAA
    GAGCTGGAAG TGACTCAGGC CACTAAAAGC AGACCGGAGC TCACTCGTCA GTTCACTGTC
    CAGAGAAGAG AGAAGGACTT TGAAGGCATG CTGGAGTACT ATAAAGAAGA TGAGGCCTTG
    CTGGTCAAGA CTTTAGTTAC AGACATGAAG CCCAACGCTG TGTCCGCCAC TGTCCCCTGC
    CTCCCCGCAT ACATCCTCTT CATGTGCATC CGTCACGCCG ACTACATCAA TGATGACCAG
    AAAGTTCATT CACTACTGAC CGCTACCATC AACGCCATCA AAAAAGTGCT GAAGAAAAAC
    AACGATGACT TTGAGATGAC CTCCTTTTGG CTGGCAAATG CAAGTCGACT TTTACACTGT
    CTGAAGCAGT ACAGCGGCGA TGAGGCGTTC ATGACCCAGA ACTCTGCCAA GCAGAACGAG
    CACTGCCTGA AGAACTTTGA CCTGGCGGAG TACCGACAGG TTCTGAGTGA CCTCTCCATC
    CAAATCTATC AGCAGCTTGT GAAAGTTGCC GAGGCCAACA TGCAGCCCAT GATCGTGTCT
    GCCATGCTTG AGAGCGAGAG CATCCCCAGC CTGGCCGGAG TGAAGCCCAT GGGTTACAGG
    AACCGCTCCT CTAGTGTGGA CTGTGAGAGT GGAGGTCCCG CAGGATACAC GCTTCAGGCA
    CTGATCAAAC AGCTGGCTCA GTTTTACTCT ACCATGGCGG ACCACGGCCT GGACCCTGAG
    ATCAGCCAGC AGGTGCTTCG CCAGCTCTTC TACTCCATCA ACGCAGTGAC CCTCAACAAC
    CTGCTGCTGC GCAAGGACGT CTGCTCCTGG AGCACCGGCA TGCAGCTCAG GTACAACATC
    AGTCAGCTGG AGGAGTGGCT AAGAGGGAAG AACCTGCACC AGTGTGGAGC TGTGGCCACT
    ATGGAGCCGG TCATTCAGGC CGCGCAGCTG CTGCAGGTGA AGAAGAAGAC ATCACAGGAC
    GCTGAAGCCA TCTGCTCGCT CTGCACTGCA CTCAGTTTAC AGCAGATTGT GAAGATTCTG
    AACTTGTACA CACCACTCAA TGAGTTTGAG GAGCGAGTCA CTGTTTCCTT CATAAGAGAC
    ATACAGAATC GCCTCCAAGA TCGAATAGAA AACAACCAAC TGCTGGCGGA CACGAAATAC
    ACCTTCCCCG TGCTCTTCCC GTACACTCCT TCCAACCTCA GCCTGGAAAC CATCCACATC
    CCAGCCTCCC TCAGCCTGGA CTTCCTGACG CGGGAGAACT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324563.1
    CDS458..5902
    Translation

    Target ORF information:

    RefSeq Version XM_021468888.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio myosin VB (myo5b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468888.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCTGTAA CTGAACTCTA CTCTAAGTAC ACCCGGGTTT GGATTCCTGA CCCGGATGAG 
    GTCTGGAGGT CTGCTGAAAT CATCAAGGAT TATAAGGAGG GAGACACAGT GCTGCATCTC
    AAATTAGAAG ATGAATCGAC GTTAGAATAT CCAATTGGTT CCAAAGACAA TCCGCTTCCA
    TTTCTCCGGA ATCCCGATAT CCTGGTAGGA GAGAATGACC TCACGGCTCT CAGTTACCTC
    CATGAGCCTG CTGTGCTCCA CAACCTCAAG GTCCGCTTCC TCGAGTCCAA CCACATCTAC
    ACCTATTGTG GGATTGTTCT GGTGGCCATC AACCCGTACG AGCAGCTACA TATCTATGGA
    GAAGAAGTCA TCAATGCATA CAGCGGGCAG AACATGGGGG ACATGGACCC TCATATATTT
    GCGGTGGCCG AGGAAGCTTT TAAACAGATG GCCAGAGATG AGAAGAACCA GTCCATAATT
    GTGAGCGGTG AGTCGGGAGC TGGTAAAACC GTTTCGGCAA AGTATGCCAT GCGCTTCTTC
    GCCACAGTGG GAGGATCAGC AAATGACACC AATGTTGAAG AGAAAGTTTT GGCTTCCAGT
    CCCATTATGG AGGCCATTGG AAATGCAAAA ACAACCCGTA ATGACAACAG CAGCCGTTTT
    GGGAAATACA TCCAGATCGG CTTTGACAGG AGATATCATA TTATTGGTGC CAATATGAGA
    ACGTACCTGC TTGAGAAGTC CAGAGTAGTG TTTCAGGCTG AAGAGGAGAG GAACTACCAC
    ATCTTCTACC AGCTCTGTGC TTCATGCTCT CTGCCAGAGT TTAAGGATCT TACCTTGACC
    AGTGCAGAAG ACTTCACTTA CACGTCTCTC GGTGAGAACA TTTTCATTGA GGGAGTGAAT
    GATGCCGAGG ACCTTGTGAA GACGAGAGAA GCTCTTACGA TGCTTGGTGT AAAAGAAAAC
    CACCAAATGA GCATCTTTAA GATAATTGCC TCCATTTTGC ACCTGGGGAA CGTGGAGATT
    GTGCAGGAGA GAGATGGAGA ATCCTGCCAT ATCAATAGGA ACGACACTCA TCTGCATCAT
    TTCTGTCGGC TGTTGGGGAT TGAGCAGGAG CAGATGGAGA ACTGGCTATG CCGCAGGAAA
    CTGGTGACCA CTTCTGAAAC TTATGTGAAA AACATGCCCC ACAGCCAGGC AGTCAATGCC
    CGAGATGCCT TGGCCAAACA CATCTATGCC CATCTGTTTG ACTGGATCGT GGAGCACATC
    AACAAGTCCC TGCATACTTC CACTAAACAG CACTCATTCA TCGGGGTTCT GGATATCTAT
    GGGTTTGAAA CATTTGAGAT CAACAGCTTT GAGCAGTTCT GTATTAACTA CGCCAATGAG
    AAGCTACAGC AGCAATTCAA CTCGCATGTG TTTAAGCTGG AGCAAGAAGA GTACATGAAG
    GAGCAGATCC CCTGGACACT CATTGATTTC TATGATAACC AGCCGTGCAT TGACCTCATT
    GAAGCCAAAC TCGGTATCCT GGACCTTCTT GATGAAGAGT GTAAGGTGCC CAAAGGAACA
    GATCAGAACT GGGCGCAAAA GCTGTACAGC AAGCATTCAG GCAGCGGCCA TTTCGAAAAG
    CCCAGGATGT CCAACAAGTC CTTTATTGTT GTTCATTTTG CAGATAAGGT TGAATATCAG
    TGTGACGGAT TCCTGGAGAA GAACAGAGAC ACTGTTTATG AAGAGCAGAT AAACATCCTC
    AAGGCCAGTA AGTTCCAGTT GGTTGCAGAT CTCTTTGACG ATGCTAAAGA TGCTGCCCCT
    CATCCAGGCA GCAAAACCTC CAGAATCAAT GTTCGGCCTG CCAAACCCGC TCCGAAAGGT
    CACAACCGGG AGCACAGGAA AACTGTGGGC ACTCAGTTCA GAAACTCTCT GCATCTTCTG
    ATGGAGACAC TCAACGCAAC CACCCCTCAT TATGTTCGCT GCATTAAGCC CAATGATTAT
    AAGGAATCCT TTGTGTTTGA TTCTAGAAGA GCTGTTCAGC AACTAAGAGC ATGTGGTGTT
    CTGGAGACCA TTCGCATTAG CGCCGCCGGC TATCCATCAC GATGGACATA TCCAGATTTC
    TTCAGCAGAT ATCGAGTGCT CATGACAAAG AAGGATCTGT CAGTCGGAGA CAAGAAGCAG
    GTCTGTAAGA ACCTGCTGGA GATCTTGATC AAGGATCCAG ATAAGTTCCA GTTTGGAAAG
    ACTAAGATCT TCTTCAGGGC GGGTCAGGTG GCCTATCTGG AGAAGCTCAG AGCCGATAAG
    TTCCGCTTCG CCTGTATTAA GATCCAGAAG ACTGTGCGAG GATGGCTGCA GAGGATTCGG
    TATCGCAAGA TTCGCAAGTC TGCCATCACT TTACAAAGAT ACGGCCGGGG TTACTTGGCA
    CGCAGGTACG CAGAGATGCT TCGCCTGACC CGAGCAGCAG TCATTTGTCA GAAGCAGTAC
    CGCATGGTAC AGGTTCGCCG GGAGTATCTG AGGGTCCGTC GGGCTGTAAT CACCATTCAA
    GCATTCACCC GAGGCATGTT CATACGCAGG TTATATCAGG AGTTCCTGCT CCATCACAAA
    GCCATGATCA TACAGAAGAC TGTCCGTGGT TGGCTTGTAA GAAAGAAGTA TCAGCGTTCC
    CGATACGCTG CCATCGTCAT TCAGTGTTTC TATCGCCGCG TACGTGCCAA GCGGCAGCTC
    AAACAGCTCA AGATAGAGGC CCGTTCAGCC GAACACTTTA AAAAACTCAA TGTCGGAATG
    GAGAACAAGA TCGTGCAGCT CCAGAAGAAG ATGGACAACC AGTCAAAAGA GTTAAAATCT
    CAGAACGAAA ATCTGGCTGT TGCTAAAACT GTTCTGGAGA CAGAGGTTAG TAAGCTGAGC
    AAAGAGCTGG AGACCCTCCG CACTCGACAG GTGGCTGGCA CTCAGATGAC ATCACTGCAG
    GAAGAGCTGG AGAAACTGAG GGCGGAGCTT CAGGAAGCAC ATGCCCACAA GAAACTTTTG
    GAGGAGGAGT TCAGCAACGA GAAGCAAGGA CTCGAACAGA GGGTGGAGGA GCTTGAGAAA
    GAAAACACAG TCTTGAAGAA GGAAAAAGAA GAGATGAACC GCAGAATCCA AACCTCTACT
    CAGGATCAGG GAGGTGATGT GTCTCAGAAG GAGAGTCGCC TGCAGCATGA ACTGGATGAC
    GAGAGACAAC GATATCAGAA CCTGGTGAAA GAATATTCCC GACTGGAGCA GAGATACGAA
    AACCTGCAGG AGGACATGTC TTCAATGAAG TTTCATCCTG GGCATCGGAG AAACCCATCT
    AACCAGAGCA GCTTGGGTTC AGACTCCAAC TATCCCTCAA TTAGCTTGTC AGAGGTGGGG
    GACACTGATG ACACCATCCA GCAGGTGGAG GAGATGGGCA TAGAGAAGGC TGCCATGGAC
    CTCAGCGTTT TCATGAAGTT ACAGAAACGT GTACGAGAGC TGGAGCAGGA GAGGAAGCGG
    CTGCAGACCA ATCTAGATAA AGTAGAGGAA CTGAGCAAAC GCAAGGGAAG CGAGGCCAAG
    AACTCAACGT CAGAGGACGA TATAAACGCA GACCTGGCCT ACAACAGTCT GAAGCGGCAA
    GAGCTCGAAA CAGAGAATAA GAAGTTGAAG AATGACCTTA ATGAGTTAAG GAAAGCAGTG
    GCCGACAGGG CCTCTGAGAA CAACTCATCC AATGAGCTTC AAGATGGCTA CAACCTGCTG
    CTCAGTCAGC TCAAAGCGGC CAATGAGGAG CTGGATGTGC GCAAGGAGGA GGTCCTGATG
    CTCAAGACCC AGATCGTCAA CTCAACAAGA CAGACAGAAA ACTTAAACCA CATCAATTCC
    AACGATGTGT CTGAGTGGAC CGACAACAAG AGCCCTGTGA ACACGGAGGA CCGCGTGGAT
    GCGCCTGCAT CAAAGAAACT GCTGGAGACC CAACTGCAGA CCCAGACTCG ACAGCACAGG
    GATGAGTTGG AAGCCCTTCA CACACAGATC GAGCTCCTGA AGGACAATCT GGAGAAGAAG
    CAGGACATGC TGGACCACTT CTCCACACTT TCTCCTGATG CTCTGGTGGA GTTCAGCGTG
    CAACAGGAGA TCACACGCCT CACCAATGAA AACCTGGACC TAAAGGAACT CGTTGAGAAG
    TTGGAAAAGA ATGAGAAGAA GCTGAAGAAG CAGTTGAGGA TCTATATGAA GAAAGTGCAA
    GAGCTGGAAG TGACTCAGGC CACTAAAAGC AGACCGGAGC TCACTCGTCA GTTCACTGTC
    CAGAGAAGAG AGAAGGACTT TGAAGGCATG CTGGAGTACT ATAAAGAAGA TGAGGCCTTG
    CTGGTCAAGA CTTTAGTTAC AGACATGAAG CCCAACGCTG TGTCCGCCAC TGTCCCCTGC
    CTCCCCGCAT ACATCCTCTT CATGTGCATC CGTCACGCCG ACTACATCAA TGATGACCAG
    AAAGTTCATT CACTACTGAC CGCTACCATC AACGCCATCA AAAAAGTGCT GAAGAAAAAC
    AACGATGACT TTGAGATGAC CTCCTTTTGG CTGGCAAATG CAAGTCGACT TTTACACTGT
    CTGAAGCAGT ACAGCGGCGA TGAGGCGTTC ATGACCCAGA ACTCTGCCAA GCAGAACGAG
    CACTGCCTGA AGAACTTTGA CCTGGCGGAG TACCGACAGG TTCTGAGTGA CCTCTCCATC
    CAAATCTATC AGCAGCTTGT GAAAGTTGCC GAGGCCAACA TGCAGCCCAT GATCGTGTCT
    GCCATGCTTG AGAGCGAGAG CATCCCCAGC CTGGCCGGAG TGAAGCCCAT GGGTTACAGG
    AACCGCTCCT CTAGTGTGGA CTGTGAGAGT GGAGGTCCCG CAGGATACAC GCTTCAGGCA
    CTGATCAAAC AGCTGGCTCA GTTTTACTCT ACCATGGCGG ACCACGGCCT GGACCCTGAG
    ATCAGCCAGC AGGTGCTTCG CCAGCTCTTC TACTCCATCA ACGCAGTGAC CCTCAACAAC
    CTGCTGCTGC GCAAGGACGT CTGCTCCTGG AGCACCGGCA TGCAGCTCAG GTACAACATC
    AGTCAGCTGG AGGAGTGGCT AAGAGGGAAG AACCTGCACC AGTGTGGAGC TGTGGCCACT
    ATGGAGCCGG TCATTCAGGC CGCGCAGCTG CTGCAGGTGA AGAAGAAGAC ATCACAGGAC
    GCTGAAGCCA TCTGCTCGCT CTGCACTGCA CTCAGTTTAC AGCAGATTGT GAAGATTCTG
    AACTTGTACA CACCACTCAA TGAGTTTGAG GAGCGAGTCA CTGTTTCCTT CATAAGAGAC
    ATACAGAATC GCCTCCAAGA TCGAATAGAA AACAACCAAC TGCTGGCGGA CACGAAATAC
    ACCTTCCCCG TGCTCTTCCC GTACACTCCT TCCAACCTCA GCCTGGAAAC CATCCACATC
    CCAGCCTCCC TCAGCCTGGA CTTCCTGACG CGGGAGAACT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45151
    Clone ID Related Accession (Same CDS sequence) NM_001161632.2
    Accession Version NM_001161632.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-14
    Organism Danio rerio(zebrafish)
    Product unconventional myosin-Vb
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU928762.7, FO704574.2 and CU466287.11. On or before Nov 25, 2014 this sequence version replaced XM_009295326.1, NM_001161632.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGTGAAC ATGAGCTTTA TGTCAAGTAC ACCCGGGTTT GGATTCCTGA CCCGGATGAG 
    GTCTGGAGGT CTGCTGAAAT CATCAAGGAT TATAAGGAGG GAGACACAGT GCTGCATCTC
    AAATTAGAAG ATGAATCGAC GTTAGAATAT CCAATTGGTT CCAAAGACAA TCCGCTTCCA
    TTTCTCCGGA ATCCCGATAT CCTGGTAGGA GAGAATGACC TCACGGCTCT CAGTTACCTC
    CATGAGCCTG CTGTGCTCCA CAACCTCAAG GTCCGCTTCC TCGAGTCCAA CCACATCTAC
    ACCTATTGTG GGATTGTTCT GGTGGCCATC AACCCGTACG AGCAGCTACA TATCTATGGA
    GAAGAAGTCA TCAATGCATA CAGCGGGCAG AACATGGGGG ACATGGACCC TCATATATTT
    GCGGTGGCCG AGGAAGCTTT TAAACAGATG GCCAGAGATG AGAAGAACCA GTCCATAATT
    GTGAGCGGTG AGTCGGGAGC TGGTAAAACC GTTTCGGCAA AGTATGCCAT GCGCTTCTTC
    GCCACAGTGG GAGGATCAGC AAATGACACC AATGTTGAAG AGAAAGTTTT GGCTTCCAGT
    CCCATTATGG AGGCCATTGG AAATGCAAAA ACAACCCGTA ATGACAACAG CAGCCGTTTT
    GGGAAATACA TCCAGATCGG CTTTGACAGG AGATATCATA TTATTGGTGC CAATATGAGA
    ACGTACCTGC TTGAGAAGTC CAGAGTAGTG TTTCAGGCTG AAGAGGAGAG GAACTACCAC
    ATCTTCTACC AGCTCTGTGC TTCATGCTCT CTGCCAGAGT TTAAGGATCT TACCTTGACC
    AGTGCAGAAG ACTTCACTTA CACGTCTCTC GGTGAGAACA TTTTCATTGA GGGAGTGAAT
    GATGCCGAGG ACCTTGTGAA GACGAGAGAA GCTCTTACGA TGCTTGGTGT AAAAGAAAAC
    CACCAAATGA GCATCTTTAA GATAATTGCC TCCATTTTGC ACCTGGGGAA CGTGGAGATT
    GTGCAGGAGA GAGATGGAGA ATCCTGCCAT ATCAATAGGA ACGACACTCA TCTGCATCAT
    TTCTGTCGGC TGTTGGGGAT TGAGCAGGAG CAGATGGAGA ACTGGCTATG CCGCAGGAAA
    CTGGTGACCA CTTCTGAAAC TTATGTGAAA AACATGCCCC ACAGCCAGGC AGTCAATGCC
    CGAGATGCCT TGGCCAAACA CATCTATGCC CATCTGTTTG ACTGGATCGT GGAGCACATC
    AACAAGTCCC TGCATACTTC CACTAAACAG CACTCATTCA TCGGGGTTCT GGATATCTAT
    GGGTTTGAAA CATTTGAGAT CAACAGCTTT GAGCAGTTCT GTATTAACTA CGCCAATGAG
    AAGCTACAGC AGCAATTCAA CTCGCATGTG TTTAAGCTGG AGCAAGAAGA GTACATGAAG
    GAGCAGATCC CCTGGACACT CATTGATTTC TATGATAACC AGCCGTGCAT TGACCTCATT
    GAAGCCAAAC TCGGTATCCT GGACCTTCTT GATGAAGAGT GTAAGGTGCC CAAAGGAACA
    GATCAGAACT GGGCGCAAAA GCTGTACAGC AAGCATTCAG GCAGCGGCCA TTTCGAAAAG
    CCCAGGATGT CCAACAAGTC CTTTATTGTT GTTCATTTTG CAGATAAGGT TGAATATCAG
    TGTGACGGAT TCCTGGAGAA GAACAGAGAC ACTGTTTATG AAGAGCAGAT AAACATCCTC
    AAGGCCAGTA AGTTCCAGTT GGTTGCAGAT CTCTTTGACG ATGCTAAAGA TGCTGCCCCT
    CATCCAGGCA GCAAAACCTC CAGAATCAAT GTTCGGCCTG CCAAACCCGC TCCGAAAGGT
    CACAACCGGG AGCACAGGAA AACTGTGGGC ACTCAGTTCA GAAACTCTCT GCATCTTCTG
    ATGGAGACAC TCAACGCAAC CACCCCTCAT TATGTTCGCT GCATTAAGCC CAATGATTAT
    AAGGAATCCT TTGTGTTTGA TTCTAGAAGA GCTGTTCAGC AACTAAGAGC ATGTGGTGTT
    CTGGAGACCA TTCGCATTAG CGCCGCCGGC TATCCATCAC GATGGACATA TCCAGATTTC
    TTCAGCAGAT ATCGAGTGCT CATGACAAAG AAGGATCTGT CAGTCGGAGA CAAGAAGCAG
    GTCTGTAAGA ACCTGCTGGA GATCTTGATC AAGGATCCAG ATAAGTTCCA GTTTGGAAAG
    ACTAAGATCT TCTTCAGGGC GGGTCAGGTG GCCTATCTGG AGAAGCTCAG AGCCGATAAG
    TTCCGCTTCG CCTGTATTAA GATCCAGAAG ACTGTGCGAG GATGGCTGCA GAGGATTCGG
    TATCGCAAGA TTCGCAAGTC TGCCATCACT TTACAAAGAT ACGGCCGGGG TTACTTGGCA
    CGCAGGTACG CAGAGATGCT TCGCCTGACC CGAGCAGCAG TCATTTGTCA GAAGCAGTAC
    CGCATGGTAC AGGTTCGCCG GGAGTATCTG AGGGTCCGTC GGGCTGTAAT CACCATTCAA
    GCATTCACCC GAGGCATGTT CATACGCAGG TTATATCAGG AGTTCCTGCT CCATCACAAA
    GCCATGATCA TACAGAAGAC TGTCCGTGGT TGGCTTGTAA GAAAGAAGTA TCAGCGTTCC
    CGATACGCTG CCATCGTCAT TCAGTGTTTC TATCGCCGCG TACGTGCCAA GCGGCAGCTC
    AAACAGCTCA AGATAGAGGC CCGTTCAGCC GAACACTTTA AAAAACTCAA TGTCGGAATG
    GAGAACAAGA TCGTGCAGCT CCAGAAGAAG ATGGACAACC AGTCAAAAGA GTTAAAATCT
    CAGAACGAAA ATCTGGCTGT TGCTAAAACT GTTCTGGAGA CAGAGGTTAG TAAGCTGAGC
    AAAGAGCTGG AGACCCTCCG CACTCGACAG GTGGCTGGCA CTCAGATGAC ATCACTGCAG
    GAAGAGCTGG AGAAACTGAG GGCGGAGCTT CAGGAAGCAC ATGCCCACAA GAAACTTTTG
    GAGGAGGAGT TCAGCAACGA GAAGCAAGGA CTCGAACAGA GGGTGGAGGA GCTTGAGAAA
    GAAAACACAG TCTTGAAGAA GGAAAAAGAA GAGATGAACC GCAGAATCCA AACCTCTACT
    CAGGATCAGG GAGGTGATGT GTCTCAGAAG GAGAGTCGCC TGCAGCATGA ACTGGATGAC
    GAGAGACAAC GATATCAGAA CCTGGTGAAA GAATATTCCC GACTGGAGCA GAGATACGAA
    AACCTGCAGG AGGACATGTC TTCAATGAAG TTTCATCCTG GGCATCGGAG AAACCCATCT
    AACCAGAGCA GCTTGGGTTC AGACTCCAAC TATCCCTCAA TTAGCTTGTC AGAGGTGGGG
    GACACTGATG ACACCATCCA GCAGGTGGAG GAGATGGGCA TAGAGAAGGC TGCCATGGAC
    CTCAGCGTTT TCATGAAGTT ACAGAAACGT GTACGAGAGC TGGAGCAGGA GAGGAAGCGG
    CTGCAGACCA ATCTAGATAA AGTAGAGGAA CTGAGCAAAC GCAAGGGAAG CGAGGCCAAG
    AACTCAACGT CAGAGGACGA TATAAACGCA GACCTGGCCT ACAACAGTCT GAAGCGGCAA
    GAGCTCGAAA CAGAGAATAA GAAGTTGAAG AATGACCTTA ATGAGTTAAG GAAAGCAGTG
    GCCGACAGGG CCTCTGAGAA CAACTCATCC AATGAGCTTC AAGATGGCTA CAACCTGCTG
    CTCAGTCAGC TCAAAGCGGC CAATGAGGAG CTGGATGTGC GCAAGGAGGA GGTCCTGATG
    CTCAAGACCC AGATCGTCAA CTCAACAAGA CAGACAGAAA ACTTAAACCA CATCAATTCC
    AACGATGTGT CTGAGTGGAC CGACAACAAG AGCCCTGTGA ACACGGAGGA CCGCGTGGAT
    GCGCCTGCAT CAAAGAATAA AGCTCAAGAC TGGGGTTATT TGAATGAAGA TGGCGAGCTA
    GGCCTGGCCT ACCAGGGTCT AAAGCAGGTT GCCAGACTGC TGGAGACCCA ACTGCAGACC
    CAGACTCGAC AGCACAGGGA TGAGTTGGAA GCCCTTCACA CACAGATCGA GCTCCTGAAG
    GACAATCTGG AGAAGAAGCA GGACATGCTG GACCACTTCT CCACACTTTC TCCTGATGCT
    CTGGTGGAGT TCAGCGTGCA ACAGGAGATC ACACGCCTCA CCAATGAAAA CCTGGACCTA
    AAGGAACTCG TTGAGAAGTT GGAAAAGAAT GAGAAGAAGC TGAAGAAGCA GTTGAGGATC
    TATATGAAGA AAGTGCAAGA GCTGGAAGTG ACTCAGGCCA CTAAAAGCAG ACCGGAGCTC
    ACTCGTCAGT TCACTGTCCA GAGAAGAGAG AAGGACTTTG AAGGCATGCT GGAGTACTAT
    AAAGAAGATG AGGCCTTGCT GGTCAAGACT TTAGTTACAG ACATGAAGCC CAACGCTGTG
    TCCGCCACTG TCCCCTGCCT CCCCGCATAC ATCCTCTTCA TGTGCATCCG TCACGCCGAC
    TACATCAATG ATGACCAGAA AGTTCATTCA CTACTGACCG CTACCATCAA CGCCATCAAA
    AAAGTGCTGA AGAAAAACAA CGATGACTTT GAGATGACCT CCTTTTGGCT GGCAAATGCA
    AGTCGACTTT TACACTGTCT GAAGCAGTAC AGCGGCGATG AGGCGTTCAT GACCCAGAAC
    TCTGCCAAGC AGAACGAGCA CTGCCTGAAG AACTTTGACC TGGCGGAGTA CCGACAGGTT
    CTGAGTGACC TCTCCATCCA AATCTATCAG CAGCTTGTGA AAGTTGCCGA GGCCAACATG
    CAGCCCATGA TCGTGTCTGC CATGCTTGAG AGCGAGAGCA TCCCCAGCCT GGCCGGAGTG
    AAGCCCATGG GTTACAGGAA CCGCTCCTCT AGTGTGGACT GTGAGAGTGG AGGTCCCGCA
    GGATACACGC TTCAGGCACT GATCAAACAG CTGGCTCAGT TTTACTCTAC CATGGCGGAC
    CACGGCCTGG ACCCTGAGAT CAGCCAGCAG GTGCTTCGCC AGCTCTTCTA CTCCATCAAC
    GCAGTGACCC TCAACAACCT GCTGCTGCGC AAGGACGTCT GCTCCTGGAG CACCGGCATG
    CAGCTCAGGT ACAACATCAG TCAGCTGGAG GAGTGGCTAA GAGGGAAGAA CCTGCACCAG
    TGTGGAGCTG TGGCCACTAT GGAGCCGGTC ATTCAGGCCG CGCAGCTGCT GCAGGTGAAG
    AAGAAGACAT CACAGGACGC TGAAGCCATC TGCTCGCTCT GCACTGCACT CAGTTTACAG
    CAGATTGTGA AGATTCTGAA CTTGTACACA CCACTCAATG AGTTTGAGGA GCGAGTCACT
    GTTTCCTTCA TAAGAGACAT ACAGAATCGC CTCCAAGATC GAATAGAAAA CAACCAACTG
    CTGGCGGACA CGAAATACAC CTTCCCCGTG CTCTTCCCGT ACACTCCTTC CAACCTCAGC
    CTGGAAACCA TCCACATCCC AGCCTCCCTC AGCCTGGACT TCCTGACGCG GGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001155104.2
    CDS145..5661
    Translation

    Target ORF information:

    RefSeq Version NM_001161632.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio myosin VB (myo5b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001161632.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGTGAAC ATGAGCTTTA TGTCAAGTAC ACCCGGGTTT GGATTCCTGA CCCGGATGAG 
    GTCTGGAGGT CTGCTGAAAT CATCAAGGAT TATAAGGAGG GAGACACAGT GCTGCATCTC
    AAATTAGAAG ATGAATCGAC GTTAGAATAT CCAATTGGTT CCAAAGACAA TCCGCTTCCA
    TTTCTCCGGA ATCCCGATAT CCTGGTAGGA GAGAATGACC TCACGGCTCT CAGTTACCTC
    CATGAGCCTG CTGTGCTCCA CAACCTCAAG GTCCGCTTCC TCGAGTCCAA CCACATCTAC
    ACCTATTGTG GGATTGTTCT GGTGGCCATC AACCCGTACG AGCAGCTACA TATCTATGGA
    GAAGAAGTCA TCAATGCATA CAGCGGGCAG AACATGGGGG ACATGGACCC TCATATATTT
    GCGGTGGCCG AGGAAGCTTT TAAACAGATG GCCAGAGATG AGAAGAACCA GTCCATAATT
    GTGAGCGGTG AGTCGGGAGC TGGTAAAACC GTTTCGGCAA AGTATGCCAT GCGCTTCTTC
    GCCACAGTGG GAGGATCAGC AAATGACACC AATGTTGAAG AGAAAGTTTT GGCTTCCAGT
    CCCATTATGG AGGCCATTGG AAATGCAAAA ACAACCCGTA ATGACAACAG CAGCCGTTTT
    GGGAAATACA TCCAGATCGG CTTTGACAGG AGATATCATA TTATTGGTGC CAATATGAGA
    ACGTACCTGC TTGAGAAGTC CAGAGTAGTG TTTCAGGCTG AAGAGGAGAG GAACTACCAC
    ATCTTCTACC AGCTCTGTGC TTCATGCTCT CTGCCAGAGT TTAAGGATCT TACCTTGACC
    AGTGCAGAAG ACTTCACTTA CACGTCTCTC GGTGAGAACA TTTTCATTGA GGGAGTGAAT
    GATGCCGAGG ACCTTGTGAA GACGAGAGAA GCTCTTACGA TGCTTGGTGT AAAAGAAAAC
    CACCAAATGA GCATCTTTAA GATAATTGCC TCCATTTTGC ACCTGGGGAA CGTGGAGATT
    GTGCAGGAGA GAGATGGAGA ATCCTGCCAT ATCAATAGGA ACGACACTCA TCTGCATCAT
    TTCTGTCGGC TGTTGGGGAT TGAGCAGGAG CAGATGGAGA ACTGGCTATG CCGCAGGAAA
    CTGGTGACCA CTTCTGAAAC TTATGTGAAA AACATGCCCC ACAGCCAGGC AGTCAATGCC
    CGAGATGCCT TGGCCAAACA CATCTATGCC CATCTGTTTG ACTGGATCGT GGAGCACATC
    AACAAGTCCC TGCATACTTC CACTAAACAG CACTCATTCA TCGGGGTTCT GGATATCTAT
    GGGTTTGAAA CATTTGAGAT CAACAGCTTT GAGCAGTTCT GTATTAACTA CGCCAATGAG
    AAGCTACAGC AGCAATTCAA CTCGCATGTG TTTAAGCTGG AGCAAGAAGA GTACATGAAG
    GAGCAGATCC CCTGGACACT CATTGATTTC TATGATAACC AGCCGTGCAT TGACCTCATT
    GAAGCCAAAC TCGGTATCCT GGACCTTCTT GATGAAGAGT GTAAGGTGCC CAAAGGAACA
    GATCAGAACT GGGCGCAAAA GCTGTACAGC AAGCATTCAG GCAGCGGCCA TTTCGAAAAG
    CCCAGGATGT CCAACAAGTC CTTTATTGTT GTTCATTTTG CAGATAAGGT TGAATATCAG
    TGTGACGGAT TCCTGGAGAA GAACAGAGAC ACTGTTTATG AAGAGCAGAT AAACATCCTC
    AAGGCCAGTA AGTTCCAGTT GGTTGCAGAT CTCTTTGACG ATGCTAAAGA TGCTGCCCCT
    CATCCAGGCA GCAAAACCTC CAGAATCAAT GTTCGGCCTG CCAAACCCGC TCCGAAAGGT
    CACAACCGGG AGCACAGGAA AACTGTGGGC ACTCAGTTCA GAAACTCTCT GCATCTTCTG
    ATGGAGACAC TCAACGCAAC CACCCCTCAT TATGTTCGCT GCATTAAGCC CAATGATTAT
    AAGGAATCCT TTGTGTTTGA TTCTAGAAGA GCTGTTCAGC AACTAAGAGC ATGTGGTGTT
    CTGGAGACCA TTCGCATTAG CGCCGCCGGC TATCCATCAC GATGGACATA TCCAGATTTC
    TTCAGCAGAT ATCGAGTGCT CATGACAAAG AAGGATCTGT CAGTCGGAGA CAAGAAGCAG
    GTCTGTAAGA ACCTGCTGGA GATCTTGATC AAGGATCCAG ATAAGTTCCA GTTTGGAAAG
    ACTAAGATCT TCTTCAGGGC GGGTCAGGTG GCCTATCTGG AGAAGCTCAG AGCCGATAAG
    TTCCGCTTCG CCTGTATTAA GATCCAGAAG ACTGTGCGAG GATGGCTGCA GAGGATTCGG
    TATCGCAAGA TTCGCAAGTC TGCCATCACT TTACAAAGAT ACGGCCGGGG TTACTTGGCA
    CGCAGGTACG CAGAGATGCT TCGCCTGACC CGAGCAGCAG TCATTTGTCA GAAGCAGTAC
    CGCATGGTAC AGGTTCGCCG GGAGTATCTG AGGGTCCGTC GGGCTGTAAT CACCATTCAA
    GCATTCACCC GAGGCATGTT CATACGCAGG TTATATCAGG AGTTCCTGCT CCATCACAAA
    GCCATGATCA TACAGAAGAC TGTCCGTGGT TGGCTTGTAA GAAAGAAGTA TCAGCGTTCC
    CGATACGCTG CCATCGTCAT TCAGTGTTTC TATCGCCGCG TACGTGCCAA GCGGCAGCTC
    AAACAGCTCA AGATAGAGGC CCGTTCAGCC GAACACTTTA AAAAACTCAA TGTCGGAATG
    GAGAACAAGA TCGTGCAGCT CCAGAAGAAG ATGGACAACC AGTCAAAAGA GTTAAAATCT
    CAGAACGAAA ATCTGGCTGT TGCTAAAACT GTTCTGGAGA CAGAGGTTAG TAAGCTGAGC
    AAAGAGCTGG AGACCCTCCG CACTCGACAG GTGGCTGGCA CTCAGATGAC ATCACTGCAG
    GAAGAGCTGG AGAAACTGAG GGCGGAGCTT CAGGAAGCAC ATGCCCACAA GAAACTTTTG
    GAGGAGGAGT TCAGCAACGA GAAGCAAGGA CTCGAACAGA GGGTGGAGGA GCTTGAGAAA
    GAAAACACAG TCTTGAAGAA GGAAAAAGAA GAGATGAACC GCAGAATCCA AACCTCTACT
    CAGGATCAGG GAGGTGATGT GTCTCAGAAG GAGAGTCGCC TGCAGCATGA ACTGGATGAC
    GAGAGACAAC GATATCAGAA CCTGGTGAAA GAATATTCCC GACTGGAGCA GAGATACGAA
    AACCTGCAGG AGGACATGTC TTCAATGAAG TTTCATCCTG GGCATCGGAG AAACCCATCT
    AACCAGAGCA GCTTGGGTTC AGACTCCAAC TATCCCTCAA TTAGCTTGTC AGAGGTGGGG
    GACACTGATG ACACCATCCA GCAGGTGGAG GAGATGGGCA TAGAGAAGGC TGCCATGGAC
    CTCAGCGTTT TCATGAAGTT ACAGAAACGT GTACGAGAGC TGGAGCAGGA GAGGAAGCGG
    CTGCAGACCA ATCTAGATAA AGTAGAGGAA CTGAGCAAAC GCAAGGGAAG CGAGGCCAAG
    AACTCAACGT CAGAGGACGA TATAAACGCA GACCTGGCCT ACAACAGTCT GAAGCGGCAA
    GAGCTCGAAA CAGAGAATAA GAAGTTGAAG AATGACCTTA ATGAGTTAAG GAAAGCAGTG
    GCCGACAGGG CCTCTGAGAA CAACTCATCC AATGAGCTTC AAGATGGCTA CAACCTGCTG
    CTCAGTCAGC TCAAAGCGGC CAATGAGGAG CTGGATGTGC GCAAGGAGGA GGTCCTGATG
    CTCAAGACCC AGATCGTCAA CTCAACAAGA CAGACAGAAA ACTTAAACCA CATCAATTCC
    AACGATGTGT CTGAGTGGAC CGACAACAAG AGCCCTGTGA ACACGGAGGA CCGCGTGGAT
    GCGCCTGCAT CAAAGAATAA AGCTCAAGAC TGGGGTTATT TGAATGAAGA TGGCGAGCTA
    GGCCTGGCCT ACCAGGGTCT AAAGCAGGTT GCCAGACTGC TGGAGACCCA ACTGCAGACC
    CAGACTCGAC AGCACAGGGA TGAGTTGGAA GCCCTTCACA CACAGATCGA GCTCCTGAAG
    GACAATCTGG AGAAGAAGCA GGACATGCTG GACCACTTCT CCACACTTTC TCCTGATGCT
    CTGGTGGAGT TCAGCGTGCA ACAGGAGATC ACACGCCTCA CCAATGAAAA CCTGGACCTA
    AAGGAACTCG TTGAGAAGTT GGAAAAGAAT GAGAAGAAGC TGAAGAAGCA GTTGAGGATC
    TATATGAAGA AAGTGCAAGA GCTGGAAGTG ACTCAGGCCA CTAAAAGCAG ACCGGAGCTC
    ACTCGTCAGT TCACTGTCCA GAGAAGAGAG AAGGACTTTG AAGGCATGCT GGAGTACTAT
    AAAGAAGATG AGGCCTTGCT GGTCAAGACT TTAGTTACAG ACATGAAGCC CAACGCTGTG
    TCCGCCACTG TCCCCTGCCT CCCCGCATAC ATCCTCTTCA TGTGCATCCG TCACGCCGAC
    TACATCAATG ATGACCAGAA AGTTCATTCA CTACTGACCG CTACCATCAA CGCCATCAAA
    AAAGTGCTGA AGAAAAACAA CGATGACTTT GAGATGACCT CCTTTTGGCT GGCAAATGCA
    AGTCGACTTT TACACTGTCT GAAGCAGTAC AGCGGCGATG AGGCGTTCAT GACCCAGAAC
    TCTGCCAAGC AGAACGAGCA CTGCCTGAAG AACTTTGACC TGGCGGAGTA CCGACAGGTT
    CTGAGTGACC TCTCCATCCA AATCTATCAG CAGCTTGTGA AAGTTGCCGA GGCCAACATG
    CAGCCCATGA TCGTGTCTGC CATGCTTGAG AGCGAGAGCA TCCCCAGCCT GGCCGGAGTG
    AAGCCCATGG GTTACAGGAA CCGCTCCTCT AGTGTGGACT GTGAGAGTGG AGGTCCCGCA
    GGATACACGC TTCAGGCACT GATCAAACAG CTGGCTCAGT TTTACTCTAC CATGGCGGAC
    CACGGCCTGG ACCCTGAGAT CAGCCAGCAG GTGCTTCGCC AGCTCTTCTA CTCCATCAAC
    GCAGTGACCC TCAACAACCT GCTGCTGCGC AAGGACGTCT GCTCCTGGAG CACCGGCATG
    CAGCTCAGGT ACAACATCAG TCAGCTGGAG GAGTGGCTAA GAGGGAAGAA CCTGCACCAG
    TGTGGAGCTG TGGCCACTAT GGAGCCGGTC ATTCAGGCCG CGCAGCTGCT GCAGGTGAAG
    AAGAAGACAT CACAGGACGC TGAAGCCATC TGCTCGCTCT GCACTGCACT CAGTTTACAG
    CAGATTGTGA AGATTCTGAA CTTGTACACA CCACTCAATG AGTTTGAGGA GCGAGTCACT
    GTTTCCTTCA TAAGAGACAT ACAGAATCGC CTCCAAGATC GAATAGAAAA CAACCAACTG
    CTGGCGGACA CGAAATACAC CTTCCCCGTG CTCTTCCCGT ACACTCCTTC CAACCTCAGC
    CTGGAAACCA TCCACATCCC AGCCTCCCTC AGCCTGGACT TCCTGACGCG GGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45148
    Clone ID Related Accession (Same CDS sequence) XM_009295325.2 , XM_009295325.3
    Accession Version XM_009295325.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product unconventional myosin-Vb isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295325.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCTGTAA CTGAACTCTA CTCTAAGTAC ACCCGGGTTT GGATTCCTGA CCCGGATGAG 
    GTCTGGAGGT CTGCTGAAAT CATCAAGGAT TATAAGGAGG GAGACACAGT GCTGCATCTC
    AAATTAGAAG ATGAATCGAC GTTAGAATAT CCAATTGGTT CCAAAGACAA TCCGCTTCCA
    TTTCTCCGGA ATCCCGATAT CCTGGTAGGA GAGAATGACC TCACGGCTCT CAGTTACCTC
    CATGAGCCTG CTGTGCTCCA CAACCTCAAG GTCCGCTTCC TCGAGTCCAA CCACATCTAC
    ACCTATTGTG GGATTGTTCT GGTGGCCATC AACCCGTACG AGCAGCTACA TATCTATGGA
    GAAGAAGTCA TCAATGCATA CAGCGGGCAG AACATGGGGG ACATGGACCC TCATATATTT
    GCGGTGGCCG AGGAAGCTTT TAAACAGATG GCCAGAGATG AGAAGAACCA GTCCATAATT
    GTGAGCGGTG AGTCGGGAGC TGGTAAAACC GTTTCGGCAA AGTATGCCAT GCGCTTCTTC
    GCCACAGTGG GAGGATCAGC AAATGACACC AATGTTGAAG AGAAAGTTTT GGCTTCCAGT
    CCCATTATGG AGGCCATTGG AAATGCAAAA ACAACCCGTA ATGACAACAG CAGCCGTTTT
    GGGAAATACA TCCAGATCGG CTTTGACAGG AGATATCATA TTATTGGTGC CAATATGAGA
    ACGTACCTGC TTGAGAAGTC CAGAGTAGTG TTTCAGGCTG AAGAGGAGAG GAACTACCAC
    ATCTTCTACC AGCTCTGTGC TTCATGCTCT CTGCCAGAGT TTAAGGATCT TACCTTGACC
    AGTGCAGAAG ACTTCACTTA CACGTCTCTC GGTGAGAACA TTTTCATTGA GGGAGTGAAT
    GATGCCGAGG ACCTTGTGAA GACGAGAGAA GCTCTTACGA TGCTTGGTGT AAAAGAAAAC
    CACCAAATGA GCATCTTTAA GATAATTGCC TCCATTTTGC ACCTGGGGAA CGTGGAGATT
    GTGCAGGAGA GAGATGGAGA ATCCTGCCAT ATCAATAGGA ACGACACTCA TCTGCATCAT
    TTCTGTCGGC TGTTGGGGAT TGAGCAGGAG CAGATGGAGA ACTGGCTATG CCGCAGGAAA
    CTGGTGACCA CTTCTGAAAC TTATGTGAAA AACATGCCCC ACAGCCAGGC AGTCAATGCC
    CGAGATGCCT TGGCCAAACA CATCTATGCC CATCTGTTTG ACTGGATCGT GGAGCACATC
    AACAAGTCCC TGCATACTTC CACTAAACAG CACTCATTCA TCGGGGTTCT GGATATCTAT
    GGGTTTGAAA CATTTGAGAT CAACAGCTTT GAGCAGTTCT GTATTAACTA CGCCAATGAG
    AAGCTACAGC AGCAATTCAA CTCGCATGTG TTTAAGCTGG AGCAAGAAGA GTACATGAAG
    GAGCAGATCC CCTGGACACT CATTGATTTC TATGATAACC AGCCGTGCAT TGACCTCATT
    GAAGCCAAAC TCGGTATCCT GGACCTTCTT GATGAAGAGT GTAAGGTGCC CAAAGGAACA
    GATCAGAACT GGGCGCAAAA GCTGTACAGC AAGCATTCAG GCAGCGGCCA TTTCGAAAAG
    CCCAGGATGT CCAACAAGTC CTTTATTGTT GTTCATTTTG CAGATAAGGT TGAATATCAG
    TGTGACGGAT TCCTGGAGAA GAACAGAGAC ACTGTTTATG AAGAGCAGAT AAACATCCTC
    AAGGCCAGTA AGTTCCAGTT GGTTGCAGAT CTCTTTGACG ATGCTAAAGA TGCTGCCCCT
    CATCCAGGCA GCAAAACCTC CAGAATCAAT GTTCGGCCTG CCAAACCCGC TCCGAAAGGT
    CACAACCGGG AGCACAGGAA AACTGTGGGC ACTCAGTTCA GAAACTCTCT GCATCTTCTG
    ATGGAGACAC TCAACGCAAC CACCCCTCAT TATGTTCGCT GCATTAAGCC CAATGATTAT
    AAGGAATCCT TTGTGTTTGA TTCTAGAAGA GCTGTTCAGC AACTAAGAGC ATGTGGTGTT
    CTGGAGACCA TTCGCATTAG CGCCGCCGGC TATCCATCAC GATGGACATA TCCAGATTTC
    TTCAGCAGAT ATCGAGTGCT CATGACAAAG AAGGATCTGT CAGTCGGAGA CAAGAAGCAG
    GTCTGTAAGA ACCTGCTGGA GATCTTGATC AAGGATCCAG ATAAGTTCCA GTTTGGAAAG
    ACTAAGATCT TCTTCAGGGC GGGTCAGGTG GCCTATCTGG AGAAGCTCAG AGCCGATAAG
    TTCCGCTTCG CCTGTATTAA GATCCAGAAG ACTGTGCGAG GATGGCTGCA GAGGATTCGG
    TATCGCAAGA TTCGCAAGTC TGCCATCACT TTACAAAGAT ACGGCCGGGG TTACTTGGCA
    CGCAGGTACG CAGAGATGCT TCGCCTGACC CGAGCAGCAG TCATTTGTCA GAAGCAGTAC
    CGCATGGTAC AGGTTCGCCG GGAGTATCTG AGGGTCCGTC GGGCTGTAAT CACCATTCAA
    GCATTCACCC GAGGCATGTT CATACGCAGG TTATATCAGG AGTTCCTGCT CCATCACAAA
    GCCATGATCA TACAGAAGAC TGTCCGTGGT TGGCTTGTAA GAAAGAAGTA TCAGCGTTCC
    CGATACGCTG CCATCGTCAT TCAGTGTTTC TATCGCCGCG TACGTGCCAA GCGGCAGCTC
    AAACAGCTCA AGATAGAGGC CCGTTCAGCC GAACACTTTA AAAAACTCAA TGTCGGAATG
    GAGAACAAGA TCGTGCAGCT CCAGAAGAAG ATGGACAACC AGTCAAAAGA GTTAAAATCT
    CAGAACGAAA ATCTGGCTGT TGCTAAAACT GTTCTGGAGA CAGAGGTTAG TAAGCTGAGC
    AAAGAGCTGG AGACCCTCCG CACTCGACAG GTGGCTGGCA CTCAGATGAC ATCACTGCAG
    GAAGAGCTGG AGAAACTGAG GGCGGAGCTT CAGGAAGCAC ATGCCCACAA GAAACTTTTG
    GAGGAGGAGT TCAGCAACGA GAAGCAAGGA CTCGAACAGA GGGTGGAGGA GCTTGAGAAA
    GAAAACACAG TCTTGAAGAA GGAAAAAGAA GAGATGAACC GCAGAATCCA AACCTCTACT
    CAGGATCAGG GAGGTGATGT GTCTCAGAAG GAGAGTCGCC TGCAGCATGA ACTGGATGAC
    GAGAGACAAC GATATCAGAA CCTGGTGAAA GAATATTCCC GACTGGAGCA GAGATACGAA
    AACCTGCAGG AGGACATGTC TTCAATGAAG TTTCATCCTG GGCATCGGAG AAACCCATCT
    AACCAGAGCA GCTTGGGTTC AGACTCCAAC TATCCCTCAA TTAGCTTGTC AGAGGTGGGG
    GACACTGATG ACACCATCCA GCAGGTGGAG GAGATGGGCA TAGAGAAGGC TGCCATGGAC
    CTCAGCGTTT TCATGAAGTT ACAGAAACGT GTACGAGAGC TGGAGCAGGA GAGGAAGCGG
    CTGCAGACCA ATCTAGATAA AGTAGAGGAA CTGAGCAAAC GCAAGGGAAG CGAGGCCAAG
    AACTCAACGT CAGAGGACGA TATAAACGCA GACCTGGCCT ACAACAGTCT GAAGCGGCAA
    GAGCTCGAAA CAGAGAATAA GAAGTTGAAG AATGACCTTA ATGAGTTAAG GAAAGCAGTG
    GCCGACAGGG CCTCTGAGAA CAACTCATCC AATGAGCTTC AAGATGGCTA CAACCTGCTG
    CTCAGTCAGC TCAAAGCGGC CAATGAGGAG CTGGATGTGC GCAAGGAGGA GGTCCTGATG
    CTCAAGACCC AGATCGTCAA CTCAACAAGA CAGACAGAAA ACTTAAACCA CATCAATTCC
    AACGATGTGT CTGAGTGGAC CGACAACAAG AGCCCTGTGA ACACGGAGGA CCGCGTGGAT
    GCGCCTGCAT CAAAGAATAA AGCTCAAGAC TGGGGTTATT TGAATGAAGA TGGCGAGCTA
    GGCCTGGCCT ACCAGGGTCT AAAGCAGGTT GCCAGACTGC TGGAGACCCA ACTGCAGACC
    CAGACTCGAC AGCACAGGGA TGAGTTGGAA GCCCTTCACA CACAGATCGA GCTCCTGAAG
    GACAATCTGG AGAAGAAGCA GGACATGCTG GACCACTTCT CCACACTTTC TCCTGATGCT
    CTGGTGGAGT TCAGCGTGCA ACAGGAGATC ACACGCCTCA CCAATGAAAA CCTGGACCTA
    AAGGAACTCG TTGAGAAGTT GGAAAAGAAT GAGAAGAAGC TGAAGAAGCA GTTGAGGATC
    TATATGAAGA AAGTGCAAGA GCTGGAAGTG ACTCAGGCCA CTAAAAGCAG ACCGGAGCTC
    ACTCGTCAGT TCACTGTCCA GAGAAGAGAG AAGGACTTTG AAGGCATGCT GGAGTACTAT
    AAAGAAGATG AGGCCTTGCT GGTCAAGACT TTAGTTACAG ACATGAAGCC CAACGCTGTG
    TCCGCCACTG TCCCCTGCCT CCCCGCATAC ATCCTCTTCA TGTGCATCCG TCACGCCGAC
    TACATCAATG ATGACCAGAA AGTTCATTCA CTACTGACCG CTACCATCAA CGCCATCAAA
    AAAGTGCTGA AGAAAAACAA CGATGACTTT GAGATGACCT CCTTTTGGCT GGCAAATGCA
    AGTCGACTTT TACACTGTCT GAAGCAGTAC AGCGGCGATG AGGCGTTCAT GACCCAGAAC
    TCTGCCAAGC AGAACGAGCA CTGCCTGAAG AACTTTGACC TGGCGGAGTA CCGACAGGTT
    CTGAGTGACC TCTCCATCCA AATCTATCAG CAGCTTGTGA AAGTTGCCGA GGCCAACATG
    CAGCCCATGA TCGTGTCTGC CATGCTTGAG AGCGAGAGCA TCCCCAGCCT GGCCGGAGTG
    AAGCCCATGG GTTACAGGAA CCGCTCCTCT AGTGTGGACT GTGAGAGTGG AGGTCCCGCA
    GGATACACGC TTCAGGCACT GATCAAACAG CTGGCTCAGT TTTACTCTAC CATGGCGGAC
    CACGGCCTGG ACCCTGAGAT CAGCCAGCAG GTGCTTCGCC AGCTCTTCTA CTCCATCAAC
    GCAGTGACCC TCAACAACCT GCTGCTGCGC AAGGACGTCT GCTCCTGGAG CACCGGCATG
    CAGCTCAGGT ACAACATCAG TCAGCTGGAG GAGTGGCTAA GAGGGAAGAA CCTGCACCAG
    TGTGGAGCTG TGGCCACTAT GGAGCCGGTC ATTCAGGCCG CGCAGCTGCT GCAGGTGAAG
    AAGAAGACAT CACAGGACGC TGAAGCCATC TGCTCGCTCT GCACTGCACT CAGTTTACAG
    CAGATTGTGA AGATTCTGAA CTTGTACACA CCACTCAATG AGTTTGAGGA GCGAGTCACT
    GTTTCCTTCA TAAGAGACAT ACAGAATCGC CTCCAAGATC GAATAGAAAA CAACCAACTG
    CTGGCGGACA CGAAATACAC CTTCCCCGTG CTCTTCCCGT ACACTCCTTC CAACCTCAGC
    CTGGAAACCA TCCACATCCC AGCCTCCCTC AGCCTGGACT TCCTGACGCG GGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293600.1
    CDS447..5963
    Translation

    Target ORF information:

    RefSeq Version XM_009295325.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio myosin VB (myo5b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295325.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCTGTAA CTGAACTCTA CTCTAAGTAC ACCCGGGTTT GGATTCCTGA CCCGGATGAG 
    GTCTGGAGGT CTGCTGAAAT CATCAAGGAT TATAAGGAGG GAGACACAGT GCTGCATCTC
    AAATTAGAAG ATGAATCGAC GTTAGAATAT CCAATTGGTT CCAAAGACAA TCCGCTTCCA
    TTTCTCCGGA ATCCCGATAT CCTGGTAGGA GAGAATGACC TCACGGCTCT CAGTTACCTC
    CATGAGCCTG CTGTGCTCCA CAACCTCAAG GTCCGCTTCC TCGAGTCCAA CCACATCTAC
    ACCTATTGTG GGATTGTTCT GGTGGCCATC AACCCGTACG AGCAGCTACA TATCTATGGA
    GAAGAAGTCA TCAATGCATA CAGCGGGCAG AACATGGGGG ACATGGACCC TCATATATTT
    GCGGTGGCCG AGGAAGCTTT TAAACAGATG GCCAGAGATG AGAAGAACCA GTCCATAATT
    GTGAGCGGTG AGTCGGGAGC TGGTAAAACC GTTTCGGCAA AGTATGCCAT GCGCTTCTTC
    GCCACAGTGG GAGGATCAGC AAATGACACC AATGTTGAAG AGAAAGTTTT GGCTTCCAGT
    CCCATTATGG AGGCCATTGG AAATGCAAAA ACAACCCGTA ATGACAACAG CAGCCGTTTT
    GGGAAATACA TCCAGATCGG CTTTGACAGG AGATATCATA TTATTGGTGC CAATATGAGA
    ACGTACCTGC TTGAGAAGTC CAGAGTAGTG TTTCAGGCTG AAGAGGAGAG GAACTACCAC
    ATCTTCTACC AGCTCTGTGC TTCATGCTCT CTGCCAGAGT TTAAGGATCT TACCTTGACC
    AGTGCAGAAG ACTTCACTTA CACGTCTCTC GGTGAGAACA TTTTCATTGA GGGAGTGAAT
    GATGCCGAGG ACCTTGTGAA GACGAGAGAA GCTCTTACGA TGCTTGGTGT AAAAGAAAAC
    CACCAAATGA GCATCTTTAA GATAATTGCC TCCATTTTGC ACCTGGGGAA CGTGGAGATT
    GTGCAGGAGA GAGATGGAGA ATCCTGCCAT ATCAATAGGA ACGACACTCA TCTGCATCAT
    TTCTGTCGGC TGTTGGGGAT TGAGCAGGAG CAGATGGAGA ACTGGCTATG CCGCAGGAAA
    CTGGTGACCA CTTCTGAAAC TTATGTGAAA AACATGCCCC ACAGCCAGGC AGTCAATGCC
    CGAGATGCCT TGGCCAAACA CATCTATGCC CATCTGTTTG ACTGGATCGT GGAGCACATC
    AACAAGTCCC TGCATACTTC CACTAAACAG CACTCATTCA TCGGGGTTCT GGATATCTAT
    GGGTTTGAAA CATTTGAGAT CAACAGCTTT GAGCAGTTCT GTATTAACTA CGCCAATGAG
    AAGCTACAGC AGCAATTCAA CTCGCATGTG TTTAAGCTGG AGCAAGAAGA GTACATGAAG
    GAGCAGATCC CCTGGACACT CATTGATTTC TATGATAACC AGCCGTGCAT TGACCTCATT
    GAAGCCAAAC TCGGTATCCT GGACCTTCTT GATGAAGAGT GTAAGGTGCC CAAAGGAACA
    GATCAGAACT GGGCGCAAAA GCTGTACAGC AAGCATTCAG GCAGCGGCCA TTTCGAAAAG
    CCCAGGATGT CCAACAAGTC CTTTATTGTT GTTCATTTTG CAGATAAGGT TGAATATCAG
    TGTGACGGAT TCCTGGAGAA GAACAGAGAC ACTGTTTATG AAGAGCAGAT AAACATCCTC
    AAGGCCAGTA AGTTCCAGTT GGTTGCAGAT CTCTTTGACG ATGCTAAAGA TGCTGCCCCT
    CATCCAGGCA GCAAAACCTC CAGAATCAAT GTTCGGCCTG CCAAACCCGC TCCGAAAGGT
    CACAACCGGG AGCACAGGAA AACTGTGGGC ACTCAGTTCA GAAACTCTCT GCATCTTCTG
    ATGGAGACAC TCAACGCAAC CACCCCTCAT TATGTTCGCT GCATTAAGCC CAATGATTAT
    AAGGAATCCT TTGTGTTTGA TTCTAGAAGA GCTGTTCAGC AACTAAGAGC ATGTGGTGTT
    CTGGAGACCA TTCGCATTAG CGCCGCCGGC TATCCATCAC GATGGACATA TCCAGATTTC
    TTCAGCAGAT ATCGAGTGCT CATGACAAAG AAGGATCTGT CAGTCGGAGA CAAGAAGCAG
    GTCTGTAAGA ACCTGCTGGA GATCTTGATC AAGGATCCAG ATAAGTTCCA GTTTGGAAAG
    ACTAAGATCT TCTTCAGGGC GGGTCAGGTG GCCTATCTGG AGAAGCTCAG AGCCGATAAG
    TTCCGCTTCG CCTGTATTAA GATCCAGAAG ACTGTGCGAG GATGGCTGCA GAGGATTCGG
    TATCGCAAGA TTCGCAAGTC TGCCATCACT TTACAAAGAT ACGGCCGGGG TTACTTGGCA
    CGCAGGTACG CAGAGATGCT TCGCCTGACC CGAGCAGCAG TCATTTGTCA GAAGCAGTAC
    CGCATGGTAC AGGTTCGCCG GGAGTATCTG AGGGTCCGTC GGGCTGTAAT CACCATTCAA
    GCATTCACCC GAGGCATGTT CATACGCAGG TTATATCAGG AGTTCCTGCT CCATCACAAA
    GCCATGATCA TACAGAAGAC TGTCCGTGGT TGGCTTGTAA GAAAGAAGTA TCAGCGTTCC
    CGATACGCTG CCATCGTCAT TCAGTGTTTC TATCGCCGCG TACGTGCCAA GCGGCAGCTC
    AAACAGCTCA AGATAGAGGC CCGTTCAGCC GAACACTTTA AAAAACTCAA TGTCGGAATG
    GAGAACAAGA TCGTGCAGCT CCAGAAGAAG ATGGACAACC AGTCAAAAGA GTTAAAATCT
    CAGAACGAAA ATCTGGCTGT TGCTAAAACT GTTCTGGAGA CAGAGGTTAG TAAGCTGAGC
    AAAGAGCTGG AGACCCTCCG CACTCGACAG GTGGCTGGCA CTCAGATGAC ATCACTGCAG
    GAAGAGCTGG AGAAACTGAG GGCGGAGCTT CAGGAAGCAC ATGCCCACAA GAAACTTTTG
    GAGGAGGAGT TCAGCAACGA GAAGCAAGGA CTCGAACAGA GGGTGGAGGA GCTTGAGAAA
    GAAAACACAG TCTTGAAGAA GGAAAAAGAA GAGATGAACC GCAGAATCCA AACCTCTACT
    CAGGATCAGG GAGGTGATGT GTCTCAGAAG GAGAGTCGCC TGCAGCATGA ACTGGATGAC
    GAGAGACAAC GATATCAGAA CCTGGTGAAA GAATATTCCC GACTGGAGCA GAGATACGAA
    AACCTGCAGG AGGACATGTC TTCAATGAAG TTTCATCCTG GGCATCGGAG AAACCCATCT
    AACCAGAGCA GCTTGGGTTC AGACTCCAAC TATCCCTCAA TTAGCTTGTC AGAGGTGGGG
    GACACTGATG ACACCATCCA GCAGGTGGAG GAGATGGGCA TAGAGAAGGC TGCCATGGAC
    CTCAGCGTTT TCATGAAGTT ACAGAAACGT GTACGAGAGC TGGAGCAGGA GAGGAAGCGG
    CTGCAGACCA ATCTAGATAA AGTAGAGGAA CTGAGCAAAC GCAAGGGAAG CGAGGCCAAG
    AACTCAACGT CAGAGGACGA TATAAACGCA GACCTGGCCT ACAACAGTCT GAAGCGGCAA
    GAGCTCGAAA CAGAGAATAA GAAGTTGAAG AATGACCTTA ATGAGTTAAG GAAAGCAGTG
    GCCGACAGGG CCTCTGAGAA CAACTCATCC AATGAGCTTC AAGATGGCTA CAACCTGCTG
    CTCAGTCAGC TCAAAGCGGC CAATGAGGAG CTGGATGTGC GCAAGGAGGA GGTCCTGATG
    CTCAAGACCC AGATCGTCAA CTCAACAAGA CAGACAGAAA ACTTAAACCA CATCAATTCC
    AACGATGTGT CTGAGTGGAC CGACAACAAG AGCCCTGTGA ACACGGAGGA CCGCGTGGAT
    GCGCCTGCAT CAAAGAATAA AGCTCAAGAC TGGGGTTATT TGAATGAAGA TGGCGAGCTA
    GGCCTGGCCT ACCAGGGTCT AAAGCAGGTT GCCAGACTGC TGGAGACCCA ACTGCAGACC
    CAGACTCGAC AGCACAGGGA TGAGTTGGAA GCCCTTCACA CACAGATCGA GCTCCTGAAG
    GACAATCTGG AGAAGAAGCA GGACATGCTG GACCACTTCT CCACACTTTC TCCTGATGCT
    CTGGTGGAGT TCAGCGTGCA ACAGGAGATC ACACGCCTCA CCAATGAAAA CCTGGACCTA
    AAGGAACTCG TTGAGAAGTT GGAAAAGAAT GAGAAGAAGC TGAAGAAGCA GTTGAGGATC
    TATATGAAGA AAGTGCAAGA GCTGGAAGTG ACTCAGGCCA CTAAAAGCAG ACCGGAGCTC
    ACTCGTCAGT TCACTGTCCA GAGAAGAGAG AAGGACTTTG AAGGCATGCT GGAGTACTAT
    AAAGAAGATG AGGCCTTGCT GGTCAAGACT TTAGTTACAG ACATGAAGCC CAACGCTGTG
    TCCGCCACTG TCCCCTGCCT CCCCGCATAC ATCCTCTTCA TGTGCATCCG TCACGCCGAC
    TACATCAATG ATGACCAGAA AGTTCATTCA CTACTGACCG CTACCATCAA CGCCATCAAA
    AAAGTGCTGA AGAAAAACAA CGATGACTTT GAGATGACCT CCTTTTGGCT GGCAAATGCA
    AGTCGACTTT TACACTGTCT GAAGCAGTAC AGCGGCGATG AGGCGTTCAT GACCCAGAAC
    TCTGCCAAGC AGAACGAGCA CTGCCTGAAG AACTTTGACC TGGCGGAGTA CCGACAGGTT
    CTGAGTGACC TCTCCATCCA AATCTATCAG CAGCTTGTGA AAGTTGCCGA GGCCAACATG
    CAGCCCATGA TCGTGTCTGC CATGCTTGAG AGCGAGAGCA TCCCCAGCCT GGCCGGAGTG
    AAGCCCATGG GTTACAGGAA CCGCTCCTCT AGTGTGGACT GTGAGAGTGG AGGTCCCGCA
    GGATACACGC TTCAGGCACT GATCAAACAG CTGGCTCAGT TTTACTCTAC CATGGCGGAC
    CACGGCCTGG ACCCTGAGAT CAGCCAGCAG GTGCTTCGCC AGCTCTTCTA CTCCATCAAC
    GCAGTGACCC TCAACAACCT GCTGCTGCGC AAGGACGTCT GCTCCTGGAG CACCGGCATG
    CAGCTCAGGT ACAACATCAG TCAGCTGGAG GAGTGGCTAA GAGGGAAGAA CCTGCACCAG
    TGTGGAGCTG TGGCCACTAT GGAGCCGGTC ATTCAGGCCG CGCAGCTGCT GCAGGTGAAG
    AAGAAGACAT CACAGGACGC TGAAGCCATC TGCTCGCTCT GCACTGCACT CAGTTTACAG
    CAGATTGTGA AGATTCTGAA CTTGTACACA CCACTCAATG AGTTTGAGGA GCGAGTCACT
    GTTTCCTTCA TAAGAGACAT ACAGAATCGC CTCCAAGATC GAATAGAAAA CAACCAACTG
    CTGGCGGACA CGAAATACAC CTTCCCCGTG CTCTTCCCGT ACACTCCTTC CAACCTCAGC
    CTGGAAACCA TCCACATCCC AGCCTCCCTC AGCCTGGACT TCCTGACGCG GGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45148
    Clone ID Related Accession (Same CDS sequence) XM_009295325.2 , XM_009295325.3
    Accession Version XM_009295325.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product unconventional myosin-Vb isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295325.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCTGTAA CTGAACTCTA CTCTAAGTAC ACCCGGGTTT GGATTCCTGA CCCGGATGAG 
    GTCTGGAGGT CTGCTGAAAT CATCAAGGAT TATAAGGAGG GAGACACAGT GCTGCATCTC
    AAATTAGAAG ATGAATCGAC GTTAGAATAT CCAATTGGTT CCAAAGACAA TCCGCTTCCA
    TTTCTCCGGA ATCCCGATAT CCTGGTAGGA GAGAATGACC TCACGGCTCT CAGTTACCTC
    CATGAGCCTG CTGTGCTCCA CAACCTCAAG GTCCGCTTCC TCGAGTCCAA CCACATCTAC
    ACCTATTGTG GGATTGTTCT GGTGGCCATC AACCCGTACG AGCAGCTACA TATCTATGGA
    GAAGAAGTCA TCAATGCATA CAGCGGGCAG AACATGGGGG ACATGGACCC TCATATATTT
    GCGGTGGCCG AGGAAGCTTT TAAACAGATG GCCAGAGATG AGAAGAACCA GTCCATAATT
    GTGAGCGGTG AGTCGGGAGC TGGTAAAACC GTTTCGGCAA AGTATGCCAT GCGCTTCTTC
    GCCACAGTGG GAGGATCAGC AAATGACACC AATGTTGAAG AGAAAGTTTT GGCTTCCAGT
    CCCATTATGG AGGCCATTGG AAATGCAAAA ACAACCCGTA ATGACAACAG CAGCCGTTTT
    GGGAAATACA TCCAGATCGG CTTTGACAGG AGATATCATA TTATTGGTGC CAATATGAGA
    ACGTACCTGC TTGAGAAGTC CAGAGTAGTG TTTCAGGCTG AAGAGGAGAG GAACTACCAC
    ATCTTCTACC AGCTCTGTGC TTCATGCTCT CTGCCAGAGT TTAAGGATCT TACCTTGACC
    AGTGCAGAAG ACTTCACTTA CACGTCTCTC GGTGAGAACA TTTTCATTGA GGGAGTGAAT
    GATGCCGAGG ACCTTGTGAA GACGAGAGAA GCTCTTACGA TGCTTGGTGT AAAAGAAAAC
    CACCAAATGA GCATCTTTAA GATAATTGCC TCCATTTTGC ACCTGGGGAA CGTGGAGATT
    GTGCAGGAGA GAGATGGAGA ATCCTGCCAT ATCAATAGGA ACGACACTCA TCTGCATCAT
    TTCTGTCGGC TGTTGGGGAT TGAGCAGGAG CAGATGGAGA ACTGGCTATG CCGCAGGAAA
    CTGGTGACCA CTTCTGAAAC TTATGTGAAA AACATGCCCC ACAGCCAGGC AGTCAATGCC
    CGAGATGCCT TGGCCAAACA CATCTATGCC CATCTGTTTG ACTGGATCGT GGAGCACATC
    AACAAGTCCC TGCATACTTC CACTAAACAG CACTCATTCA TCGGGGTTCT GGATATCTAT
    GGGTTTGAAA CATTTGAGAT CAACAGCTTT GAGCAGTTCT GTATTAACTA CGCCAATGAG
    AAGCTACAGC AGCAATTCAA CTCGCATGTG TTTAAGCTGG AGCAAGAAGA GTACATGAAG
    GAGCAGATCC CCTGGACACT CATTGATTTC TATGATAACC AGCCGTGCAT TGACCTCATT
    GAAGCCAAAC TCGGTATCCT GGACCTTCTT GATGAAGAGT GTAAGGTGCC CAAAGGAACA
    GATCAGAACT GGGCGCAAAA GCTGTACAGC AAGCATTCAG GCAGCGGCCA TTTCGAAAAG
    CCCAGGATGT CCAACAAGTC CTTTATTGTT GTTCATTTTG CAGATAAGGT TGAATATCAG
    TGTGACGGAT TCCTGGAGAA GAACAGAGAC ACTGTTTATG AAGAGCAGAT AAACATCCTC
    AAGGCCAGTA AGTTCCAGTT GGTTGCAGAT CTCTTTGACG ATGCTAAAGA TGCTGCCCCT
    CATCCAGGCA GCAAAACCTC CAGAATCAAT GTTCGGCCTG CCAAACCCGC TCCGAAAGGT
    CACAACCGGG AGCACAGGAA AACTGTGGGC ACTCAGTTCA GAAACTCTCT GCATCTTCTG
    ATGGAGACAC TCAACGCAAC CACCCCTCAT TATGTTCGCT GCATTAAGCC CAATGATTAT
    AAGGAATCCT TTGTGTTTGA TTCTAGAAGA GCTGTTCAGC AACTAAGAGC ATGTGGTGTT
    CTGGAGACCA TTCGCATTAG CGCCGCCGGC TATCCATCAC GATGGACATA TCCAGATTTC
    TTCAGCAGAT ATCGAGTGCT CATGACAAAG AAGGATCTGT CAGTCGGAGA CAAGAAGCAG
    GTCTGTAAGA ACCTGCTGGA GATCTTGATC AAGGATCCAG ATAAGTTCCA GTTTGGAAAG
    ACTAAGATCT TCTTCAGGGC GGGTCAGGTG GCCTATCTGG AGAAGCTCAG AGCCGATAAG
    TTCCGCTTCG CCTGTATTAA GATCCAGAAG ACTGTGCGAG GATGGCTGCA GAGGATTCGG
    TATCGCAAGA TTCGCAAGTC TGCCATCACT TTACAAAGAT ACGGCCGGGG TTACTTGGCA
    CGCAGGTACG CAGAGATGCT TCGCCTGACC CGAGCAGCAG TCATTTGTCA GAAGCAGTAC
    CGCATGGTAC AGGTTCGCCG GGAGTATCTG AGGGTCCGTC GGGCTGTAAT CACCATTCAA
    GCATTCACCC GAGGCATGTT CATACGCAGG TTATATCAGG AGTTCCTGCT CCATCACAAA
    GCCATGATCA TACAGAAGAC TGTCCGTGGT TGGCTTGTAA GAAAGAAGTA TCAGCGTTCC
    CGATACGCTG CCATCGTCAT TCAGTGTTTC TATCGCCGCG TACGTGCCAA GCGGCAGCTC
    AAACAGCTCA AGATAGAGGC CCGTTCAGCC GAACACTTTA AAAAACTCAA TGTCGGAATG
    GAGAACAAGA TCGTGCAGCT CCAGAAGAAG ATGGACAACC AGTCAAAAGA GTTAAAATCT
    CAGAACGAAA ATCTGGCTGT TGCTAAAACT GTTCTGGAGA CAGAGGTTAG TAAGCTGAGC
    AAAGAGCTGG AGACCCTCCG CACTCGACAG GTGGCTGGCA CTCAGATGAC ATCACTGCAG
    GAAGAGCTGG AGAAACTGAG GGCGGAGCTT CAGGAAGCAC ATGCCCACAA GAAACTTTTG
    GAGGAGGAGT TCAGCAACGA GAAGCAAGGA CTCGAACAGA GGGTGGAGGA GCTTGAGAAA
    GAAAACACAG TCTTGAAGAA GGAAAAAGAA GAGATGAACC GCAGAATCCA AACCTCTACT
    CAGGATCAGG GAGGTGATGT GTCTCAGAAG GAGAGTCGCC TGCAGCATGA ACTGGATGAC
    GAGAGACAAC GATATCAGAA CCTGGTGAAA GAATATTCCC GACTGGAGCA GAGATACGAA
    AACCTGCAGG AGGACATGTC TTCAATGAAG TTTCATCCTG GGCATCGGAG AAACCCATCT
    AACCAGAGCA GCTTGGGTTC AGACTCCAAC TATCCCTCAA TTAGCTTGTC AGAGGTGGGG
    GACACTGATG ACACCATCCA GCAGGTGGAG GAGATGGGCA TAGAGAAGGC TGCCATGGAC
    CTCAGCGTTT TCATGAAGTT ACAGAAACGT GTACGAGAGC TGGAGCAGGA GAGGAAGCGG
    CTGCAGACCA ATCTAGATAA AGTAGAGGAA CTGAGCAAAC GCAAGGGAAG CGAGGCCAAG
    AACTCAACGT CAGAGGACGA TATAAACGCA GACCTGGCCT ACAACAGTCT GAAGCGGCAA
    GAGCTCGAAA CAGAGAATAA GAAGTTGAAG AATGACCTTA ATGAGTTAAG GAAAGCAGTG
    GCCGACAGGG CCTCTGAGAA CAACTCATCC AATGAGCTTC AAGATGGCTA CAACCTGCTG
    CTCAGTCAGC TCAAAGCGGC CAATGAGGAG CTGGATGTGC GCAAGGAGGA GGTCCTGATG
    CTCAAGACCC AGATCGTCAA CTCAACAAGA CAGACAGAAA ACTTAAACCA CATCAATTCC
    AACGATGTGT CTGAGTGGAC CGACAACAAG AGCCCTGTGA ACACGGAGGA CCGCGTGGAT
    GCGCCTGCAT CAAAGAATAA AGCTCAAGAC TGGGGTTATT TGAATGAAGA TGGCGAGCTA
    GGCCTGGCCT ACCAGGGTCT AAAGCAGGTT GCCAGACTGC TGGAGACCCA ACTGCAGACC
    CAGACTCGAC AGCACAGGGA TGAGTTGGAA GCCCTTCACA CACAGATCGA GCTCCTGAAG
    GACAATCTGG AGAAGAAGCA GGACATGCTG GACCACTTCT CCACACTTTC TCCTGATGCT
    CTGGTGGAGT TCAGCGTGCA ACAGGAGATC ACACGCCTCA CCAATGAAAA CCTGGACCTA
    AAGGAACTCG TTGAGAAGTT GGAAAAGAAT GAGAAGAAGC TGAAGAAGCA GTTGAGGATC
    TATATGAAGA AAGTGCAAGA GCTGGAAGTG ACTCAGGCCA CTAAAAGCAG ACCGGAGCTC
    ACTCGTCAGT TCACTGTCCA GAGAAGAGAG AAGGACTTTG AAGGCATGCT GGAGTACTAT
    AAAGAAGATG AGGCCTTGCT GGTCAAGACT TTAGTTACAG ACATGAAGCC CAACGCTGTG
    TCCGCCACTG TCCCCTGCCT CCCCGCATAC ATCCTCTTCA TGTGCATCCG TCACGCCGAC
    TACATCAATG ATGACCAGAA AGTTCATTCA CTACTGACCG CTACCATCAA CGCCATCAAA
    AAAGTGCTGA AGAAAAACAA CGATGACTTT GAGATGACCT CCTTTTGGCT GGCAAATGCA
    AGTCGACTTT TACACTGTCT GAAGCAGTAC AGCGGCGATG AGGCGTTCAT GACCCAGAAC
    TCTGCCAAGC AGAACGAGCA CTGCCTGAAG AACTTTGACC TGGCGGAGTA CCGACAGGTT
    CTGAGTGACC TCTCCATCCA AATCTATCAG CAGCTTGTGA AAGTTGCCGA GGCCAACATG
    CAGCCCATGA TCGTGTCTGC CATGCTTGAG AGCGAGAGCA TCCCCAGCCT GGCCGGAGTG
    AAGCCCATGG GTTACAGGAA CCGCTCCTCT AGTGTGGACT GTGAGAGTGG AGGTCCCGCA
    GGATACACGC TTCAGGCACT GATCAAACAG CTGGCTCAGT TTTACTCTAC CATGGCGGAC
    CACGGCCTGG ACCCTGAGAT CAGCCAGCAG GTGCTTCGCC AGCTCTTCTA CTCCATCAAC
    GCAGTGACCC TCAACAACCT GCTGCTGCGC AAGGACGTCT GCTCCTGGAG CACCGGCATG
    CAGCTCAGGT ACAACATCAG TCAGCTGGAG GAGTGGCTAA GAGGGAAGAA CCTGCACCAG
    TGTGGAGCTG TGGCCACTAT GGAGCCGGTC ATTCAGGCCG CGCAGCTGCT GCAGGTGAAG
    AAGAAGACAT CACAGGACGC TGAAGCCATC TGCTCGCTCT GCACTGCACT CAGTTTACAG
    CAGATTGTGA AGATTCTGAA CTTGTACACA CCACTCAATG AGTTTGAGGA GCGAGTCACT
    GTTTCCTTCA TAAGAGACAT ACAGAATCGC CTCCAAGATC GAATAGAAAA CAACCAACTG
    CTGGCGGACA CGAAATACAC CTTCCCCGTG CTCTTCCCGT ACACTCCTTC CAACCTCAGC
    CTGGAAACCA TCCACATCCC AGCCTCCCTC AGCCTGGACT TCCTGACGCG GGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293600.1
    CDS459..5975
    Translation

    Target ORF information:

    RefSeq Version XM_009295325.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio myosin VB (myo5b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295325.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCTGTAA CTGAACTCTA CTCTAAGTAC ACCCGGGTTT GGATTCCTGA CCCGGATGAG 
    GTCTGGAGGT CTGCTGAAAT CATCAAGGAT TATAAGGAGG GAGACACAGT GCTGCATCTC
    AAATTAGAAG ATGAATCGAC GTTAGAATAT CCAATTGGTT CCAAAGACAA TCCGCTTCCA
    TTTCTCCGGA ATCCCGATAT CCTGGTAGGA GAGAATGACC TCACGGCTCT CAGTTACCTC
    CATGAGCCTG CTGTGCTCCA CAACCTCAAG GTCCGCTTCC TCGAGTCCAA CCACATCTAC
    ACCTATTGTG GGATTGTTCT GGTGGCCATC AACCCGTACG AGCAGCTACA TATCTATGGA
    GAAGAAGTCA TCAATGCATA CAGCGGGCAG AACATGGGGG ACATGGACCC TCATATATTT
    GCGGTGGCCG AGGAAGCTTT TAAACAGATG GCCAGAGATG AGAAGAACCA GTCCATAATT
    GTGAGCGGTG AGTCGGGAGC TGGTAAAACC GTTTCGGCAA AGTATGCCAT GCGCTTCTTC
    GCCACAGTGG GAGGATCAGC AAATGACACC AATGTTGAAG AGAAAGTTTT GGCTTCCAGT
    CCCATTATGG AGGCCATTGG AAATGCAAAA ACAACCCGTA ATGACAACAG CAGCCGTTTT
    GGGAAATACA TCCAGATCGG CTTTGACAGG AGATATCATA TTATTGGTGC CAATATGAGA
    ACGTACCTGC TTGAGAAGTC CAGAGTAGTG TTTCAGGCTG AAGAGGAGAG GAACTACCAC
    ATCTTCTACC AGCTCTGTGC TTCATGCTCT CTGCCAGAGT TTAAGGATCT TACCTTGACC
    AGTGCAGAAG ACTTCACTTA CACGTCTCTC GGTGAGAACA TTTTCATTGA GGGAGTGAAT
    GATGCCGAGG ACCTTGTGAA GACGAGAGAA GCTCTTACGA TGCTTGGTGT AAAAGAAAAC
    CACCAAATGA GCATCTTTAA GATAATTGCC TCCATTTTGC ACCTGGGGAA CGTGGAGATT
    GTGCAGGAGA GAGATGGAGA ATCCTGCCAT ATCAATAGGA ACGACACTCA TCTGCATCAT
    TTCTGTCGGC TGTTGGGGAT TGAGCAGGAG CAGATGGAGA ACTGGCTATG CCGCAGGAAA
    CTGGTGACCA CTTCTGAAAC TTATGTGAAA AACATGCCCC ACAGCCAGGC AGTCAATGCC
    CGAGATGCCT TGGCCAAACA CATCTATGCC CATCTGTTTG ACTGGATCGT GGAGCACATC
    AACAAGTCCC TGCATACTTC CACTAAACAG CACTCATTCA TCGGGGTTCT GGATATCTAT
    GGGTTTGAAA CATTTGAGAT CAACAGCTTT GAGCAGTTCT GTATTAACTA CGCCAATGAG
    AAGCTACAGC AGCAATTCAA CTCGCATGTG TTTAAGCTGG AGCAAGAAGA GTACATGAAG
    GAGCAGATCC CCTGGACACT CATTGATTTC TATGATAACC AGCCGTGCAT TGACCTCATT
    GAAGCCAAAC TCGGTATCCT GGACCTTCTT GATGAAGAGT GTAAGGTGCC CAAAGGAACA
    GATCAGAACT GGGCGCAAAA GCTGTACAGC AAGCATTCAG GCAGCGGCCA TTTCGAAAAG
    CCCAGGATGT CCAACAAGTC CTTTATTGTT GTTCATTTTG CAGATAAGGT TGAATATCAG
    TGTGACGGAT TCCTGGAGAA GAACAGAGAC ACTGTTTATG AAGAGCAGAT AAACATCCTC
    AAGGCCAGTA AGTTCCAGTT GGTTGCAGAT CTCTTTGACG ATGCTAAAGA TGCTGCCCCT
    CATCCAGGCA GCAAAACCTC CAGAATCAAT GTTCGGCCTG CCAAACCCGC TCCGAAAGGT
    CACAACCGGG AGCACAGGAA AACTGTGGGC ACTCAGTTCA GAAACTCTCT GCATCTTCTG
    ATGGAGACAC TCAACGCAAC CACCCCTCAT TATGTTCGCT GCATTAAGCC CAATGATTAT
    AAGGAATCCT TTGTGTTTGA TTCTAGAAGA GCTGTTCAGC AACTAAGAGC ATGTGGTGTT
    CTGGAGACCA TTCGCATTAG CGCCGCCGGC TATCCATCAC GATGGACATA TCCAGATTTC
    TTCAGCAGAT ATCGAGTGCT CATGACAAAG AAGGATCTGT CAGTCGGAGA CAAGAAGCAG
    GTCTGTAAGA ACCTGCTGGA GATCTTGATC AAGGATCCAG ATAAGTTCCA GTTTGGAAAG
    ACTAAGATCT TCTTCAGGGC GGGTCAGGTG GCCTATCTGG AGAAGCTCAG AGCCGATAAG
    TTCCGCTTCG CCTGTATTAA GATCCAGAAG ACTGTGCGAG GATGGCTGCA GAGGATTCGG
    TATCGCAAGA TTCGCAAGTC TGCCATCACT TTACAAAGAT ACGGCCGGGG TTACTTGGCA
    CGCAGGTACG CAGAGATGCT TCGCCTGACC CGAGCAGCAG TCATTTGTCA GAAGCAGTAC
    CGCATGGTAC AGGTTCGCCG GGAGTATCTG AGGGTCCGTC GGGCTGTAAT CACCATTCAA
    GCATTCACCC GAGGCATGTT CATACGCAGG TTATATCAGG AGTTCCTGCT CCATCACAAA
    GCCATGATCA TACAGAAGAC TGTCCGTGGT TGGCTTGTAA GAAAGAAGTA TCAGCGTTCC
    CGATACGCTG CCATCGTCAT TCAGTGTTTC TATCGCCGCG TACGTGCCAA GCGGCAGCTC
    AAACAGCTCA AGATAGAGGC CCGTTCAGCC GAACACTTTA AAAAACTCAA TGTCGGAATG
    GAGAACAAGA TCGTGCAGCT CCAGAAGAAG ATGGACAACC AGTCAAAAGA GTTAAAATCT
    CAGAACGAAA ATCTGGCTGT TGCTAAAACT GTTCTGGAGA CAGAGGTTAG TAAGCTGAGC
    AAAGAGCTGG AGACCCTCCG CACTCGACAG GTGGCTGGCA CTCAGATGAC ATCACTGCAG
    GAAGAGCTGG AGAAACTGAG GGCGGAGCTT CAGGAAGCAC ATGCCCACAA GAAACTTTTG
    GAGGAGGAGT TCAGCAACGA GAAGCAAGGA CTCGAACAGA GGGTGGAGGA GCTTGAGAAA
    GAAAACACAG TCTTGAAGAA GGAAAAAGAA GAGATGAACC GCAGAATCCA AACCTCTACT
    CAGGATCAGG GAGGTGATGT GTCTCAGAAG GAGAGTCGCC TGCAGCATGA ACTGGATGAC
    GAGAGACAAC GATATCAGAA CCTGGTGAAA GAATATTCCC GACTGGAGCA GAGATACGAA
    AACCTGCAGG AGGACATGTC TTCAATGAAG TTTCATCCTG GGCATCGGAG AAACCCATCT
    AACCAGAGCA GCTTGGGTTC AGACTCCAAC TATCCCTCAA TTAGCTTGTC AGAGGTGGGG
    GACACTGATG ACACCATCCA GCAGGTGGAG GAGATGGGCA TAGAGAAGGC TGCCATGGAC
    CTCAGCGTTT TCATGAAGTT ACAGAAACGT GTACGAGAGC TGGAGCAGGA GAGGAAGCGG
    CTGCAGACCA ATCTAGATAA AGTAGAGGAA CTGAGCAAAC GCAAGGGAAG CGAGGCCAAG
    AACTCAACGT CAGAGGACGA TATAAACGCA GACCTGGCCT ACAACAGTCT GAAGCGGCAA
    GAGCTCGAAA CAGAGAATAA GAAGTTGAAG AATGACCTTA ATGAGTTAAG GAAAGCAGTG
    GCCGACAGGG CCTCTGAGAA CAACTCATCC AATGAGCTTC AAGATGGCTA CAACCTGCTG
    CTCAGTCAGC TCAAAGCGGC CAATGAGGAG CTGGATGTGC GCAAGGAGGA GGTCCTGATG
    CTCAAGACCC AGATCGTCAA CTCAACAAGA CAGACAGAAA ACTTAAACCA CATCAATTCC
    AACGATGTGT CTGAGTGGAC CGACAACAAG AGCCCTGTGA ACACGGAGGA CCGCGTGGAT
    GCGCCTGCAT CAAAGAATAA AGCTCAAGAC TGGGGTTATT TGAATGAAGA TGGCGAGCTA
    GGCCTGGCCT ACCAGGGTCT AAAGCAGGTT GCCAGACTGC TGGAGACCCA ACTGCAGACC
    CAGACTCGAC AGCACAGGGA TGAGTTGGAA GCCCTTCACA CACAGATCGA GCTCCTGAAG
    GACAATCTGG AGAAGAAGCA GGACATGCTG GACCACTTCT CCACACTTTC TCCTGATGCT
    CTGGTGGAGT TCAGCGTGCA ACAGGAGATC ACACGCCTCA CCAATGAAAA CCTGGACCTA
    AAGGAACTCG TTGAGAAGTT GGAAAAGAAT GAGAAGAAGC TGAAGAAGCA GTTGAGGATC
    TATATGAAGA AAGTGCAAGA GCTGGAAGTG ACTCAGGCCA CTAAAAGCAG ACCGGAGCTC
    ACTCGTCAGT TCACTGTCCA GAGAAGAGAG AAGGACTTTG AAGGCATGCT GGAGTACTAT
    AAAGAAGATG AGGCCTTGCT GGTCAAGACT TTAGTTACAG ACATGAAGCC CAACGCTGTG
    TCCGCCACTG TCCCCTGCCT CCCCGCATAC ATCCTCTTCA TGTGCATCCG TCACGCCGAC
    TACATCAATG ATGACCAGAA AGTTCATTCA CTACTGACCG CTACCATCAA CGCCATCAAA
    AAAGTGCTGA AGAAAAACAA CGATGACTTT GAGATGACCT CCTTTTGGCT GGCAAATGCA
    AGTCGACTTT TACACTGTCT GAAGCAGTAC AGCGGCGATG AGGCGTTCAT GACCCAGAAC
    TCTGCCAAGC AGAACGAGCA CTGCCTGAAG AACTTTGACC TGGCGGAGTA CCGACAGGTT
    CTGAGTGACC TCTCCATCCA AATCTATCAG CAGCTTGTGA AAGTTGCCGA GGCCAACATG
    CAGCCCATGA TCGTGTCTGC CATGCTTGAG AGCGAGAGCA TCCCCAGCCT GGCCGGAGTG
    AAGCCCATGG GTTACAGGAA CCGCTCCTCT AGTGTGGACT GTGAGAGTGG AGGTCCCGCA
    GGATACACGC TTCAGGCACT GATCAAACAG CTGGCTCAGT TTTACTCTAC CATGGCGGAC
    CACGGCCTGG ACCCTGAGAT CAGCCAGCAG GTGCTTCGCC AGCTCTTCTA CTCCATCAAC
    GCAGTGACCC TCAACAACCT GCTGCTGCGC AAGGACGTCT GCTCCTGGAG CACCGGCATG
    CAGCTCAGGT ACAACATCAG TCAGCTGGAG GAGTGGCTAA GAGGGAAGAA CCTGCACCAG
    TGTGGAGCTG TGGCCACTAT GGAGCCGGTC ATTCAGGCCG CGCAGCTGCT GCAGGTGAAG
    AAGAAGACAT CACAGGACGC TGAAGCCATC TGCTCGCTCT GCACTGCACT CAGTTTACAG
    CAGATTGTGA AGATTCTGAA CTTGTACACA CCACTCAATG AGTTTGAGGA GCGAGTCACT
    GTTTCCTTCA TAAGAGACAT ACAGAATCGC CTCCAAGATC GAATAGAAAA CAACCAACTG
    CTGGCGGACA CGAAATACAC CTTCCCCGTG CTCTTCCCGT ACACTCCTTC CAACCTCAGC
    CTGGAAACCA TCCACATCCC AGCCTCCCTC AGCCTGGACT TCCTGACGCG GGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45149
    Clone ID Related Accession (Same CDS sequence) XM_009295327.2
    Accession Version XM_009295327.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product unconventional myosin-Vb isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295327.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCTGTAA CTGAACTCTA CTCTAAGTAC ACCCGGGTTT GGATTCCTGA CCCGGATGAG 
    GTCTGGAGGT CTGCTGAAAT CATCAAGGAT TATAAGGAGG GAGACACAGT GCTGCATCTC
    AAATTAGAAG ATGAATCGAC GTTAGAATAT CCAATTGGTT CCAAAGACAA TCCGCTTCCA
    TTTCTCCGGA ATCCCGATAT CCTGGTAGGA GAGAATGACC TCACGGCTCT CAGTTACCTC
    CATGAGCCTG CTGTGCTCCA CAACCTCAAG GTCCGCTTCC TCGAGTCCAA CCACATCTAC
    ACCTATTGTG GGATTGTTCT GGTGGCCATC AACCCGTACG AGCAGCTACA TATCTATGGA
    GAAGAAGTCA TCAATGCATA CAGCGGGCAG AACATGGGGG ACATGGACCC TCATATATTT
    GCGGTGGCCG AGGAAGCTTT TAAACAGATG GCCAGAGATG AGAAGAACCA GTCCATAATT
    GTGAGCGGTG AGTCGGGAGC TGGTAAAACC GTTTCGGCAA AGTATGCCAT GCGCTTCTTC
    GCCACAGTGG GAGGATCAGC AAATGACACC AATGTTGAAG AGAAAGTTTT GGCTTCCAGT
    CCCATTATGG AGGCCATTGG AAATGCAAAA ACAACCCGTA ATGACAACAG CAGCCGTTTT
    GGGAAATACA TCCAGATCGG CTTTGACAGG AGATATCATA TTATTGGTGC CAATATGAGA
    ACGTACCTGC TTGAGAAGTC CAGAGTAGTG TTTCAGGCTG AAGAGGAGAG GAACTACCAC
    ATCTTCTACC AGCTCTGTGC TTCATGCTCT CTGCCAGAGT TTAAGGATCT TACCTTGACC
    AGTGCAGAAG ACTTCACTTA CACGTCTCTC GGTGAGAACA TTTTCATTGA GGGAGTGAAT
    GATGCCGAGG ACCTTGTGAA GACGAGAGAA GCTCTTACGA TGCTTGGTGT AAAAGAAAAC
    CACCAAATGA GCATCTTTAA GATAATTGCC TCCATTTTGC ACCTGGGGAA CGTGGAGATT
    GTGCAGGAGA GAGATGGAGA ATCCTGCCAT ATCAATAGGA ACGACACTCA TCTGCATCAT
    TTCTGTCGGC TGTTGGGGAT TGAGCAGGAG CAGATGGAGA ACTGGCTATG CCGCAGGAAA
    CTGGTGACCA CTTCTGAAAC TTATGTGAAA AACATGCCCC ACAGCCAGGC AGTCAATGCC
    CGAGATGCCT TGGCCAAACA CATCTATGCC CATCTGTTTG ACTGGATCGT GGAGCACATC
    AACAAGTCCC TGCATACTTC CACTAAACAG CACTCATTCA TCGGGGTTCT GGATATCTAT
    GGGTTTGAAA CATTTGAGAT CAACAGCTTT GAGCAGTTCT GTATTAACTA CGCCAATGAG
    AAGCTACAGC AGCAATTCAA CTCGCATGTG TTTAAGCTGG AGCAAGAAGA GTACATGAAG
    GAGCAGATCC CCTGGACACT CATTGATTTC TATGATAACC AGCCGTGCAT TGACCTCATT
    GAAGCCAAAC TCGGTATCCT GGACCTTCTT GATGAAGAGT GTAAGGTGCC CAAAGGAACA
    GATCAGAACT GGGCGCAAAA GCTGTACAGC AAGCATTCAG GCAGCGGCCA TTTCGAAAAG
    CCCAGGATGT CCAACAAGTC CTTTATTGTT GTTCATTTTG CAGATAAGGT TGAATATCAG
    TGTGACGGAT TCCTGGAGAA GAACAGAGAC ACTGTTTATG AAGAGCAGAT AAACATCCTC
    AAGGCCAGTA AGTTCCAGTT GGTTGCAGAT CTCTTTGACG ATGCTAAAGA TGCTGCCCCT
    CATCCAGGCA GCAAAACCTC CAGAATCAAT GTTCGGCCTG CCAAACCCGC TCCGAAAGGT
    CACAACCGGG AGCACAGGAA AACTGTGGGC ACTCAGTTCA GAAACTCTCT GCATCTTCTG
    ATGGAGACAC TCAACGCAAC CACCCCTCAT TATGTTCGCT GCATTAAGCC CAATGATTAT
    AAGGAATCCT TTGTGTTTGA TTCTAGAAGA GCTGTTCAGC AACTAAGAGC ATGTGGTGTT
    CTGGAGACCA TTCGCATTAG CGCCGCCGGC TATCCATCAC GATGGACATA TCCAGATTTC
    TTCAGCAGAT ATCGAGTGCT CATGACAAAG AAGGATCTGT CAGTCGGAGA CAAGAAGCAG
    GTCTGTAAGA ACCTGCTGGA GATCTTGATC AAGGATCCAG ATAAGTTCCA GTTTGGAAAG
    ACTAAGATCT TCTTCAGGGC GGGTCAGGTG GCCTATCTGG AGAAGCTCAG AGCCGATAAG
    TTCCGCTTCG CCTGTATTAA GATCCAGAAG ACTGTGCGAG GATGGCTGCA GAGGATTCGG
    TATCGCAAGA TTCGCAAGTC TGCCATCACT TTACAAAGAT ACGGCCGGGG TTACTTGGCA
    CGCAGGTACG CAGAGATGCT TCGCCTGACC CGAGCAGCAG TCATTTGTCA GAAGCAGTAC
    CGCATGGTAC AGGTTCGCCG GGAGTATCTG AGGGTCCGTC GGGCTGTAAT CACCATTCAA
    GCATTCACCC GAGGCATGTT CATACGCAGG TTATATCAGG AGTTCCTGCT CCATCACAAA
    GCCATGATCA TACAGAAGAC TGTCCGTGGT TGGCTTGTAA GAAAGAAGTA TCAGCGTTCC
    CGATACGCTG CCATCGTCAT TCAGTGTTTC TATCGCCGCG TACGTGCCAA GCGGCAGCTC
    AAACAGCTCA AGATAGAGGC CCGTTCAGCC GAACACTTTA AAAAACTCAA TGTCGGAATG
    GAGAACAAGA TCGTGCAGCT CCAGAAGAAG ATGGACAACC AGTCAAAAGA GTTAAAATCT
    CAGAACGAAA ATCTGGCTGT TGCTAAAACT GTTCTGGAGA CAGAGGTTAG TAAGCTGAGC
    AAAGAGCTGG AGACCCTCCG CACTCGACAG GTGGCTGGCA CTCAGATGAC ATCACTGCAG
    GAAGAGCTGG AGAAACTGAG GGCGGAGCTT CAGGAAGCAC ATGCCCACAA GAAACTTTTG
    GAGGAGGAGT TCAGCAACGA GAAGCAAGGA CTCGAACAGA GGGTGGAGGA GCTTGAGAAA
    GAAAACACAG TCTTGAAGAA GGAAAAAGAA GAGATGAACC GCAGAATCCA AACCTCTACT
    CAGGATCAGG GAGGTGATGT GTCTCAGAAG GAGAGTCGCC TGCAGCATGA ACTGGATGAC
    GAGAGACAAC GATATCAGAA CCTGGTGAAA GAATATTCCC GACTGGAGCA GAGATACGAA
    AACCTGCAGG AGGACATGTC TTCAATGAAG TTTCATCCTG GGCATCGGAG AAACCCATCT
    AACCAGAGCA GCTTGGGTTC AGACTCCAAC TATCCCTCAA TTAGCTTGTC AGAGGTGGGG
    GACACTGATG ACACCATCCA GCAGGTGGAG GAGATGGGCA TAGAGAAGGC TGCCATGGAC
    CTCAGCGTTT TCATGAAGTT ACAGAAACGT GTACGAGAGC TGGAGCAGGA GAGGAAGCGG
    CTGCAGACCA ATCTAGATAA AGTAGAGGAA CTGAGCAAAC GCAAGGGAAG CGAGGCCAAG
    AACTCAACGT CAGAGGACGA TATAAACGCA GACCTGGCCT ACAACAGTCT GAAGCGGCAA
    GAGCTCGAAA CAGAGAATAA GAAGTTGAAG AATGACCTTA ATGAGTTAAG GAAAGCAGTG
    GCCGACAGGG CCTCTGAGAA CAACTCATCC AATGAGCTTC AAGATGGCTA CAACCTGCTG
    CTCAGTCAGC TCAAAGCGGC CAATGAGGAG CTGGATGTGC GCAAGGAGGA GGTCCTGATG
    CTCAAGACCC AGATCGTCAA CTCAACAAGA CAGACAGAAA ACTTAAACCA CATCAATTCC
    AACGATGTGT CTGAGTGGAC CGACAACAAG AGCCCTGTGA ACACGGAGGA CCGCGTGGAT
    GCGCCTGCAT CAAAGAAACT GCTGGAGACC CAACTGCAGA CCCAGACTCG ACAGCACAGG
    GATGAGTTGG AAGCCCTTCA CACACAGATC GAGCTCCTGA AGGACAATCT GGAGAAGAAG
    CAGGACATGC TGGACCACTT CTCCACACTT TCTCCTGATG CTCTGGTGGA GTTCAGCGTG
    CAACAGGAGA TCACACGCCT CACCAATGAA AACCTGGACC TAAAGGAACT CGTTGAGAAG
    TTGGAAAAGA ATGAGAAGAA GCTGAAGAAG CAGTTGAGGA TCTATATGAA GAAAGTGCAA
    GAGCTGGAAG TGACTCAGGC CACTAAAAGC AGACCGGAGC TCACTCGTCA GTTCACTGTC
    CAGAGAAGAG AGAAGGACTT TGAAGGCATG CTGGAGTACT ATAAAGAAGA TGAGGCCTTG
    CTGGTCAAGA CTTTAGTTAC AGACATGAAG CCCAACGCTG TGTCCGCCAC TGTCCCCTGC
    CTCCCCGCAT ACATCCTCTT CATGTGCATC CGTCACGCCG ACTACATCAA TGATGACCAG
    AAAGTTCATT CACTACTGAC CGCTACCATC AACGCCATCA AAAAAGTGCT GAAGAAAAAC
    AACGATGACT TTGAGATGAC CTCCTTTTGG CTGGCAAATG CAAGTCGACT TTTACACTGT
    CTGAAGCAGT ACAGCGGCGA TGAGGCGTTC ATGACCCAGA ACTCTGCCAA GCAGAACGAG
    CACTGCCTGA AGAACTTTGA CCTGGCGGAG TACCGACAGG TTCTGAGTGA CCTCTCCATC
    CAAATCTATC AGCAGCTTGT GAAAGTTGCC GAGGCCAACA TGCAGCCCAT GATCGTGTCT
    GCCATGCTTG AGAGCGAGAG CATCCCCAGC CTGGCCGGAG TGAAGCCCAT GGGTTACAGG
    AACCGCTCCT CTAGTGTGGA CTGTGAGAGT GGAGGTCCCG CAGGATACAC GCTTCAGGCA
    CTGATCAAAC AGCTGGCTCA GTTTTACTCT ACCATGGCGG ACCACGGCCT GGACCCTGAG
    ATCAGCCAGC AGGTGCTTCG CCAGCTCTTC TACTCCATCA ACGCAGTGAC CCTCAACAAC
    CTGCTGCTGC GCAAGGACGT CTGCTCCTGG AGCACCGGCA TGCAGCTCAG GTACAACATC
    AGTCAGCTGG AGGAGTGGCT AAGAGGGAAG AACCTGCACC AGTGTGGAGC TGTGGCCACT
    ATGGAGCCGG TCATTCAGGC CGCGCAGCTG CTGCAGGTGA AGAAGAAGAC ATCACAGGAC
    GCTGAAGCCA TCTGCTCGCT CTGCACTGCA CTCAGTTTAC AGCAGATTGT GAAGATTCTG
    AACTTGTACA CACCACTCAA TGAGTTTGAG GAGCGAGTCA CTGTTTCCTT CATAAGAGAC
    ATACAGAATC GCCTCCAAGA TCGAATAGAA AACAACCAAC TGCTGGCGGA CACGAAATAC
    ACCTTCCCCG TGCTCTTCCC GTACACTCCT TCCAACCTCA GCCTGGAAAC CATCCACATC
    CCAGCCTCCC TCAGCCTGGA CTTCCTGACG CGGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293602.1
    CDS447..5885
    Translation

    Target ORF information:

    RefSeq Version XM_009295327.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio myosin VB (myo5b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295327.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCTGTAA CTGAACTCTA CTCTAAGTAC ACCCGGGTTT GGATTCCTGA CCCGGATGAG 
    GTCTGGAGGT CTGCTGAAAT CATCAAGGAT TATAAGGAGG GAGACACAGT GCTGCATCTC
    AAATTAGAAG ATGAATCGAC GTTAGAATAT CCAATTGGTT CCAAAGACAA TCCGCTTCCA
    TTTCTCCGGA ATCCCGATAT CCTGGTAGGA GAGAATGACC TCACGGCTCT CAGTTACCTC
    CATGAGCCTG CTGTGCTCCA CAACCTCAAG GTCCGCTTCC TCGAGTCCAA CCACATCTAC
    ACCTATTGTG GGATTGTTCT GGTGGCCATC AACCCGTACG AGCAGCTACA TATCTATGGA
    GAAGAAGTCA TCAATGCATA CAGCGGGCAG AACATGGGGG ACATGGACCC TCATATATTT
    GCGGTGGCCG AGGAAGCTTT TAAACAGATG GCCAGAGATG AGAAGAACCA GTCCATAATT
    GTGAGCGGTG AGTCGGGAGC TGGTAAAACC GTTTCGGCAA AGTATGCCAT GCGCTTCTTC
    GCCACAGTGG GAGGATCAGC AAATGACACC AATGTTGAAG AGAAAGTTTT GGCTTCCAGT
    CCCATTATGG AGGCCATTGG AAATGCAAAA ACAACCCGTA ATGACAACAG CAGCCGTTTT
    GGGAAATACA TCCAGATCGG CTTTGACAGG AGATATCATA TTATTGGTGC CAATATGAGA
    ACGTACCTGC TTGAGAAGTC CAGAGTAGTG TTTCAGGCTG AAGAGGAGAG GAACTACCAC
    ATCTTCTACC AGCTCTGTGC TTCATGCTCT CTGCCAGAGT TTAAGGATCT TACCTTGACC
    AGTGCAGAAG ACTTCACTTA CACGTCTCTC GGTGAGAACA TTTTCATTGA GGGAGTGAAT
    GATGCCGAGG ACCTTGTGAA GACGAGAGAA GCTCTTACGA TGCTTGGTGT AAAAGAAAAC
    CACCAAATGA GCATCTTTAA GATAATTGCC TCCATTTTGC ACCTGGGGAA CGTGGAGATT
    GTGCAGGAGA GAGATGGAGA ATCCTGCCAT ATCAATAGGA ACGACACTCA TCTGCATCAT
    TTCTGTCGGC TGTTGGGGAT TGAGCAGGAG CAGATGGAGA ACTGGCTATG CCGCAGGAAA
    CTGGTGACCA CTTCTGAAAC TTATGTGAAA AACATGCCCC ACAGCCAGGC AGTCAATGCC
    CGAGATGCCT TGGCCAAACA CATCTATGCC CATCTGTTTG ACTGGATCGT GGAGCACATC
    AACAAGTCCC TGCATACTTC CACTAAACAG CACTCATTCA TCGGGGTTCT GGATATCTAT
    GGGTTTGAAA CATTTGAGAT CAACAGCTTT GAGCAGTTCT GTATTAACTA CGCCAATGAG
    AAGCTACAGC AGCAATTCAA CTCGCATGTG TTTAAGCTGG AGCAAGAAGA GTACATGAAG
    GAGCAGATCC CCTGGACACT CATTGATTTC TATGATAACC AGCCGTGCAT TGACCTCATT
    GAAGCCAAAC TCGGTATCCT GGACCTTCTT GATGAAGAGT GTAAGGTGCC CAAAGGAACA
    GATCAGAACT GGGCGCAAAA GCTGTACAGC AAGCATTCAG GCAGCGGCCA TTTCGAAAAG
    CCCAGGATGT CCAACAAGTC CTTTATTGTT GTTCATTTTG CAGATAAGGT TGAATATCAG
    TGTGACGGAT TCCTGGAGAA GAACAGAGAC ACTGTTTATG AAGAGCAGAT AAACATCCTC
    AAGGCCAGTA AGTTCCAGTT GGTTGCAGAT CTCTTTGACG ATGCTAAAGA TGCTGCCCCT
    CATCCAGGCA GCAAAACCTC CAGAATCAAT GTTCGGCCTG CCAAACCCGC TCCGAAAGGT
    CACAACCGGG AGCACAGGAA AACTGTGGGC ACTCAGTTCA GAAACTCTCT GCATCTTCTG
    ATGGAGACAC TCAACGCAAC CACCCCTCAT TATGTTCGCT GCATTAAGCC CAATGATTAT
    AAGGAATCCT TTGTGTTTGA TTCTAGAAGA GCTGTTCAGC AACTAAGAGC ATGTGGTGTT
    CTGGAGACCA TTCGCATTAG CGCCGCCGGC TATCCATCAC GATGGACATA TCCAGATTTC
    TTCAGCAGAT ATCGAGTGCT CATGACAAAG AAGGATCTGT CAGTCGGAGA CAAGAAGCAG
    GTCTGTAAGA ACCTGCTGGA GATCTTGATC AAGGATCCAG ATAAGTTCCA GTTTGGAAAG
    ACTAAGATCT TCTTCAGGGC GGGTCAGGTG GCCTATCTGG AGAAGCTCAG AGCCGATAAG
    TTCCGCTTCG CCTGTATTAA GATCCAGAAG ACTGTGCGAG GATGGCTGCA GAGGATTCGG
    TATCGCAAGA TTCGCAAGTC TGCCATCACT TTACAAAGAT ACGGCCGGGG TTACTTGGCA
    CGCAGGTACG CAGAGATGCT TCGCCTGACC CGAGCAGCAG TCATTTGTCA GAAGCAGTAC
    CGCATGGTAC AGGTTCGCCG GGAGTATCTG AGGGTCCGTC GGGCTGTAAT CACCATTCAA
    GCATTCACCC GAGGCATGTT CATACGCAGG TTATATCAGG AGTTCCTGCT CCATCACAAA
    GCCATGATCA TACAGAAGAC TGTCCGTGGT TGGCTTGTAA GAAAGAAGTA TCAGCGTTCC
    CGATACGCTG CCATCGTCAT TCAGTGTTTC TATCGCCGCG TACGTGCCAA GCGGCAGCTC
    AAACAGCTCA AGATAGAGGC CCGTTCAGCC GAACACTTTA AAAAACTCAA TGTCGGAATG
    GAGAACAAGA TCGTGCAGCT CCAGAAGAAG ATGGACAACC AGTCAAAAGA GTTAAAATCT
    CAGAACGAAA ATCTGGCTGT TGCTAAAACT GTTCTGGAGA CAGAGGTTAG TAAGCTGAGC
    AAAGAGCTGG AGACCCTCCG CACTCGACAG GTGGCTGGCA CTCAGATGAC ATCACTGCAG
    GAAGAGCTGG AGAAACTGAG GGCGGAGCTT CAGGAAGCAC ATGCCCACAA GAAACTTTTG
    GAGGAGGAGT TCAGCAACGA GAAGCAAGGA CTCGAACAGA GGGTGGAGGA GCTTGAGAAA
    GAAAACACAG TCTTGAAGAA GGAAAAAGAA GAGATGAACC GCAGAATCCA AACCTCTACT
    CAGGATCAGG GAGGTGATGT GTCTCAGAAG GAGAGTCGCC TGCAGCATGA ACTGGATGAC
    GAGAGACAAC GATATCAGAA CCTGGTGAAA GAATATTCCC GACTGGAGCA GAGATACGAA
    AACCTGCAGG AGGACATGTC TTCAATGAAG TTTCATCCTG GGCATCGGAG AAACCCATCT
    AACCAGAGCA GCTTGGGTTC AGACTCCAAC TATCCCTCAA TTAGCTTGTC AGAGGTGGGG
    GACACTGATG ACACCATCCA GCAGGTGGAG GAGATGGGCA TAGAGAAGGC TGCCATGGAC
    CTCAGCGTTT TCATGAAGTT ACAGAAACGT GTACGAGAGC TGGAGCAGGA GAGGAAGCGG
    CTGCAGACCA ATCTAGATAA AGTAGAGGAA CTGAGCAAAC GCAAGGGAAG CGAGGCCAAG
    AACTCAACGT CAGAGGACGA TATAAACGCA GACCTGGCCT ACAACAGTCT GAAGCGGCAA
    GAGCTCGAAA CAGAGAATAA GAAGTTGAAG AATGACCTTA ATGAGTTAAG GAAAGCAGTG
    GCCGACAGGG CCTCTGAGAA CAACTCATCC AATGAGCTTC AAGATGGCTA CAACCTGCTG
    CTCAGTCAGC TCAAAGCGGC CAATGAGGAG CTGGATGTGC GCAAGGAGGA GGTCCTGATG
    CTCAAGACCC AGATCGTCAA CTCAACAAGA CAGACAGAAA ACTTAAACCA CATCAATTCC
    AACGATGTGT CTGAGTGGAC CGACAACAAG AGCCCTGTGA ACACGGAGGA CCGCGTGGAT
    GCGCCTGCAT CAAAGAAACT GCTGGAGACC CAACTGCAGA CCCAGACTCG ACAGCACAGG
    GATGAGTTGG AAGCCCTTCA CACACAGATC GAGCTCCTGA AGGACAATCT GGAGAAGAAG
    CAGGACATGC TGGACCACTT CTCCACACTT TCTCCTGATG CTCTGGTGGA GTTCAGCGTG
    CAACAGGAGA TCACACGCCT CACCAATGAA AACCTGGACC TAAAGGAACT CGTTGAGAAG
    TTGGAAAAGA ATGAGAAGAA GCTGAAGAAG CAGTTGAGGA TCTATATGAA GAAAGTGCAA
    GAGCTGGAAG TGACTCAGGC CACTAAAAGC AGACCGGAGC TCACTCGTCA GTTCACTGTC
    CAGAGAAGAG AGAAGGACTT TGAAGGCATG CTGGAGTACT ATAAAGAAGA TGAGGCCTTG
    CTGGTCAAGA CTTTAGTTAC AGACATGAAG CCCAACGCTG TGTCCGCCAC TGTCCCCTGC
    CTCCCCGCAT ACATCCTCTT CATGTGCATC CGTCACGCCG ACTACATCAA TGATGACCAG
    AAAGTTCATT CACTACTGAC CGCTACCATC AACGCCATCA AAAAAGTGCT GAAGAAAAAC
    AACGATGACT TTGAGATGAC CTCCTTTTGG CTGGCAAATG CAAGTCGACT TTTACACTGT
    CTGAAGCAGT ACAGCGGCGA TGAGGCGTTC ATGACCCAGA ACTCTGCCAA GCAGAACGAG
    CACTGCCTGA AGAACTTTGA CCTGGCGGAG TACCGACAGG TTCTGAGTGA CCTCTCCATC
    CAAATCTATC AGCAGCTTGT GAAAGTTGCC GAGGCCAACA TGCAGCCCAT GATCGTGTCT
    GCCATGCTTG AGAGCGAGAG CATCCCCAGC CTGGCCGGAG TGAAGCCCAT GGGTTACAGG
    AACCGCTCCT CTAGTGTGGA CTGTGAGAGT GGAGGTCCCG CAGGATACAC GCTTCAGGCA
    CTGATCAAAC AGCTGGCTCA GTTTTACTCT ACCATGGCGG ACCACGGCCT GGACCCTGAG
    ATCAGCCAGC AGGTGCTTCG CCAGCTCTTC TACTCCATCA ACGCAGTGAC CCTCAACAAC
    CTGCTGCTGC GCAAGGACGT CTGCTCCTGG AGCACCGGCA TGCAGCTCAG GTACAACATC
    AGTCAGCTGG AGGAGTGGCT AAGAGGGAAG AACCTGCACC AGTGTGGAGC TGTGGCCACT
    ATGGAGCCGG TCATTCAGGC CGCGCAGCTG CTGCAGGTGA AGAAGAAGAC ATCACAGGAC
    GCTGAAGCCA TCTGCTCGCT CTGCACTGCA CTCAGTTTAC AGCAGATTGT GAAGATTCTG
    AACTTGTACA CACCACTCAA TGAGTTTGAG GAGCGAGTCA CTGTTTCCTT CATAAGAGAC
    ATACAGAATC GCCTCCAAGA TCGAATAGAA AACAACCAAC TGCTGGCGGA CACGAAATAC
    ACCTTCCCCG TGCTCTTCCC GTACACTCCT TCCAACCTCA GCCTGGAAAC CATCCACATC
    CCAGCCTCCC TCAGCCTGGA CTTCCTGACG CGGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.