MYO5B cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYO5B
Entrez Gene ID	514078
Full Name	myosin VB
General protein information	Preferred Names myosin VB Names unconventional myosin-Vb
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024984592.1	XP_024840360.1	unconventional myosin-Vb isoform X2
XM_015469177.1	XP_015324663.1	unconventional myosin-Vb isoform X2
XM_015460147.1	XP_015315633.1	unconventional myosin-Vb isoform X2
XM_002697821.5	XP_002697867.2	unconventional myosin-Vb isoform X1
XM_015469176.1	XP_015324662.1	unconventional myosin-Vb isoform X1
XM_002697821.4	XP_002697867.2	unconventional myosin-Vb isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	myo5b	XP_002936554.2
Pan troglodytes (chimpanzee)	MYO5B	XP_003953333.1
Oryza sativa (rice)	Os02g0777700	NP_001048291.2
	MYO5B	XP_001090434.1
Canis lupus familiaris (dog)	MYO5B	XP_005623182.1
Bos taurus (cattle)	MYO5B	XP_002697867.2
Mus musculus (house mouse)	Myo5b	NP_963894.1
Danio rerio (zebrafish)	myo5b	NP_001155104.1
Schizosaccharomyces pombe (fission yeast)	myo52	NP_588492.1
Arabidopsis thaliana (thale cress)	XI-I	NP_195046.3
Homo sapiens (human)	MYO5B	NP_001073936.1
Rattus norvegicus (Norway rat)	Myo5b	NP_058779.1
Danio rerio (zebrafish)	LOC100536519	XP_003200915.2

Gene Ontology

The following MYO5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYO5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa32664	XM_002697821.5 Latest version!	Bos taurus myosin VB (MYO5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa32664	XM_015469176.1 Latest version!	Bos taurus myosin VB (MYO5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa48797	XM_024984592.1 Latest version!	Bos taurus myosin VB (MYO5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa88362	XM_015469177.1 Latest version!	Bos taurus myosin VB (MYO5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa88362	XM_015460147.1 Latest version!	Bos taurus myosin VB (MYO5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OBa32664	XM_002697821.4	Bos taurus myosin VB (MYO5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYO5B ( XM_002697821.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697867.2 Bos taurus myosin VB (MYO5B), transcript variant X1, mRNA.

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CloneID	OBa32664
Clone ID Related Accession (Same CDS sequence)	XM_002697821.5 , XM_015469176.1 , XM_002697821.4
Accession Version	XM_002697821.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	unconventional myosin-Vb isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002697821.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCA GCGAGCTCTA CAGCCAGTAC ACAAGGGTCT GGATCCCTGA CCCGGATGAG 
GTGTGGCGCT CCGCTGAATT AACCAAGGAC TACAAGGAGG GGGACAAGAG CCTCCAGCTC 
CGACTGGAAG ATGAAACCAT TCAGGAGTAC CCCATCGATG TCCAAAACAA CCAACTTCCC 
TTCCTGCGGA ATCCAGATAT CCTGGTGGGA GAAAATGACC TAACTGCCCT TAGCTATCTC 
CATGAGCCTG CAGTTTTGCA TAATTTAAAG GTCCGCTTCC TGGAGTCCAA CCATATCTAC 
ACTTACTGTG GGATTGTGCT TGTTGCCATT AATCCTTATG ATCAGTTGCC AATCTACGGT 
CAAGATGTCA TCTATGCCTA CAGTGGCCAG AACATGGGGG ATATGGACCC CCACATCTTT 
GCTGTGGCAG AGGAAGCCTA CAAGCAGATG GCCAGAGATG AGAAGAACCA GTCCATCATA 
GTCAGTGGGG AGTCTGGAGC TGGCAAGACG GTGTCGGCCA AGTATGCCAT GCGCTACTTC 
ACCACGGTCA GTGGCTCAGC CAGTGACACC AACATCGAAG AGAAAGTCCT GGCGTCCAGT 
CCCATCATGG AGGCCATCGG GAATGCTAAA ACCACCCGCA ACGACAACAG CAGCCGTTTC 
GGGAAGTACA TTCAGATCGG CTTTGACAAG AGGTACCGCA TCATCGGGGC CAACATGAGG 
ACCTACCTGC TGGAGAAGTC CAGGGTGGTC TTTCAGGCAG ACGACGAGAG GAATTACCAC 
ATCTTTTACC AGCTCTGTGC GGCTGCCAGT CTTCCGGAAT TTAAGGAGCT GGCACTAACC 
TGTGCTGAGG ACTTCTTCTA CACGTCACAG GGAGGAAACA CAGTCATCGA GGGTGTGGAT 
GATGCTGAGG ACTTTGAGAA GACTCGACAA GCCTTCACGC TCGTTGGAGT GAGAGAGTCC 
CATCAGATAA GCATTTTTAA GATAATTGCT TCCATCCTGC ACCTCGGAAA TGTGGAGATT 
CAAGCCGAGC GCGATGGTGA ATCCTGTAGA GTATCACCTG AGGATGAACA CCTAAGCGAC 
TTCTGCCGCC TGCTGGGCGT GGAGCTCAGT CAGATGGAGC ACTGGCTGTG CCATCGCAAG 
CTGGTCACCA CCTCGGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAGGT GGTCAACGCG 
CGCGACGCCC TGGCCAAGCA CATCTATGCC CAGCTGTTCC ACTGGATCGT GGAGCACGTC 
AACAAGGCCC TGCACACCTC CCTCAAGCAG CACTCCTTCA TTGGAGTCCT GGACATCTAC 
GGGTTTGAGA CGTTTGAGGT GAACAGCTTC GAGCAGTTCT GTATCAACTA CGCCAATGAG 
AAGCTCCAGC AGCAGTTCAA CTCACACGTA TTCAAGCTGG AGCAGGAGGA GTACATGAAG 
GAGCAGATCC CGTGGACCCT GATTGATTTC TATGATAACC AGCCTTGTAT CGACCTCATA 
GAAGCTAAGC TGGGCATCTT GGACCTGTTG GATGAAGAAT GTAAGGTCCC CAAAGGAACC 
GACCAGAACT GGACTCAGAA GCTCTATGAC AGGCACTCTG GCAGCCAACA CTTCCAGAAA 
CCCCGCATGT CCAACAAGGC CTTCATTGTC ATCCACTTTG CAGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTGGAGAA AAACAGAGAT ACCGTGTACG AAGAACAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCGCT GGTGGCTGAC TTGTTCCATG ATGGCAAAGA CTCTGCTCCC 
ACCGCCACCG CTTCTTCCAA GATCAACATT CGCTCCTCCA GACCCCCTGT GAAAGCCTCC 
AACAAGGAGC ACAAGAAAAC CGTGGGTTAC CAGTTCCGCT CCTCCCTGCA TCTGCTCATG 
GAGACACTGA ACGCCACGAC ACCGCACTAT GTCCGCTGCA TCAAGCCCAA CGACGAGAAG 
CTGCCCTTCC ATTTTAACCC AAAGAGAGCA GTGCAGCAGC TCCGAGCCTG CGGCGTATTG 
GAGACCATCC GGATCAGCGC GGCTGGCTAC CCATCCAGGT GGTCCTACCA TGACTTCTTC 
AACCGGTATC GGGTGCTGGT CAAGAAGAGA GACCTTGCCA ACTCAGACAA AAAGGCCATC 
TGCAGGTCTG TGCTGGAGAG CCTCATCAAG GATCCTGACA AGTTCCAGTT TGGCCGCACC 
AAGATCTTCT TCCGGGCCGG CCAGGTGGCC TACCTGGAGA AGCTTCGGGC TGACAAGTTC 
CGGGCGGCCA CCATCATGAT CCAGAAAGCC GTGCGGGGCT GGCTGCAGAA GGTGAAGTAC 
CGCAGGTTGA AGGGAGCCAC CTTAATTCTG CAGAGGTACT GCCGAGGATA CCTGGCCCGC 
AGACTGGCCA AGCATCTGCG GAGGACCCGG GCGGCCGTGG TGCTCCAGAA GCAATACCGC 
ATGCAGAGGG CCCGCCGGGC CTACCAGAGG GTCCGCAGGG CCGCCGTCGT CATCCAGGCC 
TTTGCTCGGG GCATGTTTGT GCGGAGAATC TACCACCAGG TCCTCAGGGA GCACAAGGCC 
ACCATCATCC AGAAGCACGT GCGGGGCTGG ATGGCTCGCA GGCGCTTCCA GCGGCTGCGG 
GGTGCGGCCG TTGTCATCCA GTGCGGCTTC CGGAGGCTCA AGGCCAAGCA GGCGCTGAAG 
GCCCTCAGGA TCGAGGCGCG CTCCGCGGAG CACCTGAAGC GCCTCAACGT GGGCATGGAG 
AACAAGATTG TCCAGCTGCA GCGGAAGATC GATGACCAGA ACAAAGAGCT CAAGACGCTG 
TCGGAGCAGC TGTCAGCAAT CACCTCCACA CACACCATGG AAGTGGAGAA GCTGAAGAAG 
GAGGTGGCCT GCTACCAGCA GAGCCAGGGT GAGGACAGAG GCCCGCAGTT GCAGGAGGAG 
GTGGAGAGCC TGCGGACCGA GCTGCAGAGG GCCCACTCAG AGCGCAAGGT CCTGGAGGAC 
ACCCACACCC GGGAGAAGGA TGAGCTGAGG AAGCGAGTTG CAGACCTGGA ACAAGAAAAT 
GCACTCTTGA AAAATGAGAA GGAGCAGCTT AACAACCAGA TCCTGTGCCA GTCTAAAGAT 
GAGTTTGCCC AGAACTCTGT CAAGGAAAAC CTCATGATGA AGAAGGAGCT GGAGGAGGAG 
CGCTCCCGTT ATCAGAACCT CGTGAAGGAG TATTCCCGGC TGGAGCAGAG ATACGACAAC 
CTTCGGGATG AGATGACCAT CCTAAAGCAA ACCCCAGGCC ACCGGAGGAA CCCATCAAAC 
CAAAGCAGCT TAGAATCTGA CTCCAATTAC CCGTCCATCT CCACGTCTGA GGTGGGAGAC 
ACAGAGGACA CCCTCCAGCA GGTGGAGGAA ATTGGCCTGG AGAAGGCAGC CATGGACATG 
ACCGTCTTCC TGAAGCTGCA GAAGAGGGTG CGGGAGCTGG AGCAGGAGAG GAAGAAGCTG 
CAGGTGCAGC TGGAGAAGAA AGAACAGCAG GACGGCAAGA GAGTCCCGGC GGACCAACCA 
AGCAATGACT CAGATTTGGA CCAGGATGCA GATCTAGCCT ACAACAGTCT GAAGAGGCAG 
GAGCTGGAGT CGGAGAACAA GAAGCTGAAG AATGAGCTGA ACGAGCTGAG GAAAGCCGTG 
GCCGACCAAG CCTCGGAGCA CAGCCCGGCT GCCGCCGGCA CCCCCGACAG CTACAGCCTC 
CTGCTGAACC AGCTCCGGGT GGCCAACGAG GAGCTGGAGG TGCGCAAGGA GGAAGTTCTC 
ATCCTGCGGA CCCAGATCGT GAGCGCCGAC CAGCGCCGCC TGGCGGGCAA GGGCTCGGAG 
CCAAACATCA ATGCCCGAAC CAGTTGGCCG AACAGCGAGA AGCACGTGGA TCAGGAAGAT 
GCCATCGAGG CCTATCACGG GGTCTGCCAG ACTAACAGCA AGACTGAGGA TTGGGGCTAC 
TTGAATGAAG ATGGAGAGCT CGGCTTGGCC TACCAAGGCC TAAAGCAAGT TGCCAGGTTG 
CTGGAGGCAC AGCTGCAGGC CCAGAGCCGC GAGCACGAGG AGGAGGTGGA CAATCTCAAG 
GCCCAGCTGG AGGCCCTGAA GGAGGAGATG GACAGACAGC AGCAGACCTT CTGCCAGACC 
CTGCTGCTCT CCCCAGAGGC CCAGGTGGAG TTCGGCATCC AGCAGGAGAT GTCTCGGCTC 
ACCAACGAGA ACCTGGACCT TAAAGAGTTA GTTGAGAAGC TGGAGAAGAA CGAGAGGAAG 
CTCAAAAAGC AACTGAAGAT TTACATGAAG AAGGTCCAGG ACCTGGAAGC TGCCCAAGCC 
TTGGCCCAGA GCGAGAGGAA GCGGCACGAG CTCAACAGGC AGGTCACCGT GCAGCGGAAG 
GAGAAGGACT TCCAAGGCAT GCTGGAGTAC CACAAGGAGG ACGAGGCCCT GCTCATTCGG 
AATCTGGTGA CAGAGTTGAA ACCGCAGGTG CTGGCGGGCG CGGTGCCCTG CCTCCCAGCC 
TACATCCTCT ACATGTGCCT CCGGCACGCG GACTACGTGA ACGACGACCT CAAGGTGCAC 
TCCCTGCTGA CCTCCACCAT CAACGGCATC AAGAAGGTCC TCAAGAAGCA CAATGAGGAC 
TTTGAGATGA CGTCGTTCTG GTTGTCCAAC ACCTGCCGCC TCCTGCACTG CTTGAAGCAG 
TACAGCGGGG ACGAGGGCTT CATGACTCAG AACACTGCAA AGCAGAATGA GCACTGTCTT 
AAGAACTTTG ACCTCACTGA ATACCGCCAG GTGCTGAGCG ATCTTTCCAT TCAGATCTAC 
CAGCAGCTCA TTAAAATCGC CGAGGGCCTC TTGCAGCCTA TGATAGTGTC GGCCATGTTG 
GAGAACGAGA GCATTCAGGG TCTGTCTGGG GTGAAGCCCA CGGGCTACCG GAAGCGCACC 
TCCAGCATGC CGGAAGGAGA CAACTCGTAC TGCCTGGAAG CCATCATCCG CCAGATGAAC 
TCCTTCCACA CGGTTATGTG CGACCAGGGC CTGGACCCCG AGATCATCCT GCAGGTGTTC 
AGACAGCTCT TCTACATGAT CAACGCGGTG ACGCTCAACA ACCTGCTTCT GCGGAAGGAC 
GTCTGCTCCT GGAGCACAGG CATGCAGCTC AGGTACAACA TTAGTCAGCT GGAGGAATGG 
CTTCGGGGAA GAAACCTGCA CCAGAGCGGA GCTGTTGAGA CCATGGAACC CCTGATTCAG 
GCTGCCCAGC TCCTGCAGTT AAAGAAGAAA AGCCCCGAGG ATGCTGAGGC CATCTGCTCC 
CTGTGCACCG CCCTCAGCAC CCAGCAGATT GTCAAAATTT TGAACCTTTA TACCCCTCTG 
AATGAATTTG AAGAACGGGT GACAGTGGCT TTTATACGAA CAATCCAGGC ACAACTGCAA 
GATCGGAATG ACCCTCAGCA GCTGCTATTA GACTTCAAGC ACATGTTTCC TGTTTTGTTC 
CCGTTTAATC CATCTTCTCT GACCATGGAC TCAATCCACA TCCCAGCATG TCTCAATCTG 
GAGTTCCTCA ACGAAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697867.2
CDS	241..5781
Translation	MSVSELYSQYTRVWIPDPDEVWRSAELTKDYKEGDKSLQLRLEDETIQEYPIDVQNNQLPFLRNPDILVGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYDQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFTTVSGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYRIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTSQGGNTVIEGVDDAEDFEKTRQAFTLVGVRESHQISIFKIIASILHLGNVEIQAERDGESCRVSPEDEHLSDFCRLLGVELSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARDALAKHIYAQLFHWIVEHVNKALHTSLKQHSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWTQKLYDRHSGSQHFQKPRMSNKAFIVIHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDGKDSAPTATASSKINIRSSRPPVKASNKEHKKTVGYQFRSSLHLLMETLNATTPHYVRCIKPNDEKLPFHFNPKRAVQQLRACGVLETIRISAAGYPSRWSYHDFFNRYRVLVKKRDLANSDKKAICRSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRAATIMIQKAVRGWLQKVKYRRLKGATLILQRYCRGYLARRLAKHLRRTRAAVVLQKQYRMQRARRAYQRVRRAAVVIQAFARGMFVRRIYHQVLREHKATIIQKHVRGWMARRRFQRLRGAAVVIQCGFRRLKAKQALKALRIEARSAEHLKRLNVGMENKIVQLQRKIDDQNKELKTLSEQLSAITSTHTMEVEKLKKEVACYQQSQGEDRGPQLQEEVESLRTELQRAHSERKVLEDTHTREKDELRKRVADLEQENALLKNEKEQLNNQILCQSKDEFAQNSVKENLMMKKELEEERSRYQNLVKEYSRLEQRYDNLRDEMTILKQTPGHRRNPSNQSSLESDSNYPSISTSEVGDTEDTLQQVEEIGLEKAAMDMTVFLKLQKRVRELEQERKKLQVQLEKKEQQDGKRVPADQPSNDSDLDQDADLAYNSLKRQELESENKKLKNELNELRKAVADQASEHSPAAAGTPDSYSLLLNQLRVANEELEVRKEEVLILRTQIVSADQRRLAGKGSEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNSKTEDWGYLNEDGELGLAYQGLKQVARLLEAQLQAQSREHEEEVDNLKAQLEALKEEMDRQQQTFCQTLLLSPEAQVEFGIQQEMSRLTNENLDLKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSERKRHELNRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTELKPQVLAGAVPCLPAYILYMCLRHADYVNDDLKVHSLLTSTINGIKKVLKKHNEDFEMTSFWLSNTCRLLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGLLQPMIVSAMLENESIQGLSGVKPTGYRKRTSSMPEGDNSYCLEAIIRQMNSFHTVMCDQGLDPEIILQVFRQLFYMINAVTLNNLLLRKDVCSWSTGMQLRYNISQLEEWLRGRNLHQSGAVETMEPLIQAAQLLQLKKKSPEDAEAICSLCTALSTQQIVKILNLYTPLNEFEERVTVAFIRTIQAQLQDRNDPQQLLLDFKHMFPVLFPFNPSSLTMDSIHIPACLNLEFLNEV

Target ORF information:

RefSeq Version	XM_002697821.5
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VB (MYO5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697821.5

ORF Insert Sequence:

ATGTCGGTCA GCGAGCTCTA CAGCCAGTAC ACAAGGGTCT GGATCCCTGA CCCGGATGAG 
GTGTGGCGCT CCGCTGAATT AACCAAGGAC TACAAGGAGG GGGACAAGAG CCTCCAGCTC 
CGACTGGAAG ATGAAACCAT TCAGGAGTAC CCCATCGATG TCCAAAACAA CCAACTTCCC 
TTCCTGCGGA ATCCAGATAT CCTGGTGGGA GAAAATGACC TAACTGCCCT TAGCTATCTC 
CATGAGCCTG CAGTTTTGCA TAATTTAAAG GTCCGCTTCC TGGAGTCCAA CCATATCTAC 
ACTTACTGTG GGATTGTGCT TGTTGCCATT AATCCTTATG ATCAGTTGCC AATCTACGGT 
CAAGATGTCA TCTATGCCTA CAGTGGCCAG AACATGGGGG ATATGGACCC CCACATCTTT 
GCTGTGGCAG AGGAAGCCTA CAAGCAGATG GCCAGAGATG AGAAGAACCA GTCCATCATA 
GTCAGTGGGG AGTCTGGAGC TGGCAAGACG GTGTCGGCCA AGTATGCCAT GCGCTACTTC 
ACCACGGTCA GTGGCTCAGC CAGTGACACC AACATCGAAG AGAAAGTCCT GGCGTCCAGT 
CCCATCATGG AGGCCATCGG GAATGCTAAA ACCACCCGCA ACGACAACAG CAGCCGTTTC 
GGGAAGTACA TTCAGATCGG CTTTGACAAG AGGTACCGCA TCATCGGGGC CAACATGAGG 
ACCTACCTGC TGGAGAAGTC CAGGGTGGTC TTTCAGGCAG ACGACGAGAG GAATTACCAC 
ATCTTTTACC AGCTCTGTGC GGCTGCCAGT CTTCCGGAAT TTAAGGAGCT GGCACTAACC 
TGTGCTGAGG ACTTCTTCTA CACGTCACAG GGAGGAAACA CAGTCATCGA GGGTGTGGAT 
GATGCTGAGG ACTTTGAGAA GACTCGACAA GCCTTCACGC TCGTTGGAGT GAGAGAGTCC 
CATCAGATAA GCATTTTTAA GATAATTGCT TCCATCCTGC ACCTCGGAAA TGTGGAGATT 
CAAGCCGAGC GCGATGGTGA ATCCTGTAGA GTATCACCTG AGGATGAACA CCTAAGCGAC 
TTCTGCCGCC TGCTGGGCGT GGAGCTCAGT CAGATGGAGC ACTGGCTGTG CCATCGCAAG 
CTGGTCACCA CCTCGGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAGGT GGTCAACGCG 
CGCGACGCCC TGGCCAAGCA CATCTATGCC CAGCTGTTCC ACTGGATCGT GGAGCACGTC 
AACAAGGCCC TGCACACCTC CCTCAAGCAG CACTCCTTCA TTGGAGTCCT GGACATCTAC 
GGGTTTGAGA CGTTTGAGGT GAACAGCTTC GAGCAGTTCT GTATCAACTA CGCCAATGAG 
AAGCTCCAGC AGCAGTTCAA CTCACACGTA TTCAAGCTGG AGCAGGAGGA GTACATGAAG 
GAGCAGATCC CGTGGACCCT GATTGATTTC TATGATAACC AGCCTTGTAT CGACCTCATA 
GAAGCTAAGC TGGGCATCTT GGACCTGTTG GATGAAGAAT GTAAGGTCCC CAAAGGAACC 
GACCAGAACT GGACTCAGAA GCTCTATGAC AGGCACTCTG GCAGCCAACA CTTCCAGAAA 
CCCCGCATGT CCAACAAGGC CTTCATTGTC ATCCACTTTG CAGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTGGAGAA AAACAGAGAT ACCGTGTACG AAGAACAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCGCT GGTGGCTGAC TTGTTCCATG ATGGCAAAGA CTCTGCTCCC 
ACCGCCACCG CTTCTTCCAA GATCAACATT CGCTCCTCCA GACCCCCTGT GAAAGCCTCC 
AACAAGGAGC ACAAGAAAAC CGTGGGTTAC CAGTTCCGCT CCTCCCTGCA TCTGCTCATG 
GAGACACTGA ACGCCACGAC ACCGCACTAT GTCCGCTGCA TCAAGCCCAA CGACGAGAAG 
CTGCCCTTCC ATTTTAACCC AAAGAGAGCA GTGCAGCAGC TCCGAGCCTG CGGCGTATTG 
GAGACCATCC GGATCAGCGC GGCTGGCTAC CCATCCAGGT GGTCCTACCA TGACTTCTTC 
AACCGGTATC GGGTGCTGGT CAAGAAGAGA GACCTTGCCA ACTCAGACAA AAAGGCCATC 
TGCAGGTCTG TGCTGGAGAG CCTCATCAAG GATCCTGACA AGTTCCAGTT TGGCCGCACC 
AAGATCTTCT TCCGGGCCGG CCAGGTGGCC TACCTGGAGA AGCTTCGGGC TGACAAGTTC 
CGGGCGGCCA CCATCATGAT CCAGAAAGCC GTGCGGGGCT GGCTGCAGAA GGTGAAGTAC 
CGCAGGTTGA AGGGAGCCAC CTTAATTCTG CAGAGGTACT GCCGAGGATA CCTGGCCCGC 
AGACTGGCCA AGCATCTGCG GAGGACCCGG GCGGCCGTGG TGCTCCAGAA GCAATACCGC 
ATGCAGAGGG CCCGCCGGGC CTACCAGAGG GTCCGCAGGG CCGCCGTCGT CATCCAGGCC 
TTTGCTCGGG GCATGTTTGT GCGGAGAATC TACCACCAGG TCCTCAGGGA GCACAAGGCC 
ACCATCATCC AGAAGCACGT GCGGGGCTGG ATGGCTCGCA GGCGCTTCCA GCGGCTGCGG 
GGTGCGGCCG TTGTCATCCA GTGCGGCTTC CGGAGGCTCA AGGCCAAGCA GGCGCTGAAG 
GCCCTCAGGA TCGAGGCGCG CTCCGCGGAG CACCTGAAGC GCCTCAACGT GGGCATGGAG 
AACAAGATTG TCCAGCTGCA GCGGAAGATC GATGACCAGA ACAAAGAGCT CAAGACGCTG 
TCGGAGCAGC TGTCAGCAAT CACCTCCACA CACACCATGG AAGTGGAGAA GCTGAAGAAG 
GAGGTGGCCT GCTACCAGCA GAGCCAGGGT GAGGACAGAG GCCCGCAGTT GCAGGAGGAG 
GTGGAGAGCC TGCGGACCGA GCTGCAGAGG GCCCACTCAG AGCGCAAGGT CCTGGAGGAC 
ACCCACACCC GGGAGAAGGA TGAGCTGAGG AAGCGAGTTG CAGACCTGGA ACAAGAAAAT 
GCACTCTTGA AAAATGAGAA GGAGCAGCTT AACAACCAGA TCCTGTGCCA GTCTAAAGAT 
GAGTTTGCCC AGAACTCTGT CAAGGAAAAC CTCATGATGA AGAAGGAGCT GGAGGAGGAG 
CGCTCCCGTT ATCAGAACCT CGTGAAGGAG TATTCCCGGC TGGAGCAGAG ATACGACAAC 
CTTCGGGATG AGATGACCAT CCTAAAGCAA ACCCCAGGCC ACCGGAGGAA CCCATCAAAC 
CAAAGCAGCT TAGAATCTGA CTCCAATTAC CCGTCCATCT CCACGTCTGA GGTGGGAGAC 
ACAGAGGACA CCCTCCAGCA GGTGGAGGAA ATTGGCCTGG AGAAGGCAGC CATGGACATG 
ACCGTCTTCC TGAAGCTGCA GAAGAGGGTG CGGGAGCTGG AGCAGGAGAG GAAGAAGCTG 
CAGGTGCAGC TGGAGAAGAA AGAACAGCAG GACGGCAAGA GAGTCCCGGC GGACCAACCA 
AGCAATGACT CAGATTTGGA CCAGGATGCA GATCTAGCCT ACAACAGTCT GAAGAGGCAG 
GAGCTGGAGT CGGAGAACAA GAAGCTGAAG AATGAGCTGA ACGAGCTGAG GAAAGCCGTG 
GCCGACCAAG CCTCGGAGCA CAGCCCGGCT GCCGCCGGCA CCCCCGACAG CTACAGCCTC 
CTGCTGAACC AGCTCCGGGT GGCCAACGAG GAGCTGGAGG TGCGCAAGGA GGAAGTTCTC 
ATCCTGCGGA CCCAGATCGT GAGCGCCGAC CAGCGCCGCC TGGCGGGCAA GGGCTCGGAG 
CCAAACATCA ATGCCCGAAC CAGTTGGCCG AACAGCGAGA AGCACGTGGA TCAGGAAGAT 
GCCATCGAGG CCTATCACGG GGTCTGCCAG ACTAACAGCA AGACTGAGGA TTGGGGCTAC 
TTGAATGAAG ATGGAGAGCT CGGCTTGGCC TACCAAGGCC TAAAGCAAGT TGCCAGGTTG 
CTGGAGGCAC AGCTGCAGGC CCAGAGCCGC GAGCACGAGG AGGAGGTGGA CAATCTCAAG 
GCCCAGCTGG AGGCCCTGAA GGAGGAGATG GACAGACAGC AGCAGACCTT CTGCCAGACC 
CTGCTGCTCT CCCCAGAGGC CCAGGTGGAG TTCGGCATCC AGCAGGAGAT GTCTCGGCTC 
ACCAACGAGA ACCTGGACCT TAAAGAGTTA GTTGAGAAGC TGGAGAAGAA CGAGAGGAAG 
CTCAAAAAGC AACTGAAGAT TTACATGAAG AAGGTCCAGG ACCTGGAAGC TGCCCAAGCC 
TTGGCCCAGA GCGAGAGGAA GCGGCACGAG CTCAACAGGC AGGTCACCGT GCAGCGGAAG 
GAGAAGGACT TCCAAGGCAT GCTGGAGTAC CACAAGGAGG ACGAGGCCCT GCTCATTCGG 
AATCTGGTGA CAGAGTTGAA ACCGCAGGTG CTGGCGGGCG CGGTGCCCTG CCTCCCAGCC 
TACATCCTCT ACATGTGCCT CCGGCACGCG GACTACGTGA ACGACGACCT CAAGGTGCAC 
TCCCTGCTGA CCTCCACCAT CAACGGCATC AAGAAGGTCC TCAAGAAGCA CAATGAGGAC 
TTTGAGATGA CGTCGTTCTG GTTGTCCAAC ACCTGCCGCC TCCTGCACTG CTTGAAGCAG 
TACAGCGGGG ACGAGGGCTT CATGACTCAG AACACTGCAA AGCAGAATGA GCACTGTCTT 
AAGAACTTTG ACCTCACTGA ATACCGCCAG GTGCTGAGCG ATCTTTCCAT TCAGATCTAC 
CAGCAGCTCA TTAAAATCGC CGAGGGCCTC TTGCAGCCTA TGATAGTGTC GGCCATGTTG 
GAGAACGAGA GCATTCAGGG TCTGTCTGGG GTGAAGCCCA CGGGCTACCG GAAGCGCACC 
TCCAGCATGC CGGAAGGAGA CAACTCGTAC TGCCTGGAAG CCATCATCCG CCAGATGAAC 
TCCTTCCACA CGGTTATGTG CGACCAGGGC CTGGACCCCG AGATCATCCT GCAGGTGTTC 
AGACAGCTCT TCTACATGAT CAACGCGGTG ACGCTCAACA ACCTGCTTCT GCGGAAGGAC 
GTCTGCTCCT GGAGCACAGG CATGCAGCTC AGGTACAACA TTAGTCAGCT GGAGGAATGG 
CTTCGGGGAA GAAACCTGCA CCAGAGCGGA GCTGTTGAGA CCATGGAACC CCTGATTCAG 
GCTGCCCAGC TCCTGCAGTT AAAGAAGAAA AGCCCCGAGG ATGCTGAGGC CATCTGCTCC 
CTGTGCACCG CCCTCAGCAC CCAGCAGATT GTCAAAATTT TGAACCTTTA TACCCCTCTG 
AATGAATTTG AAGAACGGGT GACAGTGGCT TTTATACGAA CAATCCAGGC ACAACTGCAA 
GATCGGAATG ACCCTCAGCA GCTGCTATTA GACTTCAAGC ACATGTTTCC TGTTTTGTTC 
CCGTTTAATC CATCTTCTCT GACCATGGAC TCAATCCACA TCCCAGCATG TCTCAATCTG 
GAGTTCCTCA ACGAAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5B ( XM_015469176.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324662.1 Bos taurus myosin VB (MYO5B), transcript variant X1, mRNA.

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CloneID	OBa32664
Clone ID Related Accession (Same CDS sequence)	XM_002697821.5 , XM_015469176.1 , XM_002697821.4
Accession Version	XM_015469176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Vb isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCA GCGAGCTCTA CAGCCAGTAC ACAAGGGTCT GGATCCCTGA CCCGGATGAG 
GTGTGGCGCT CCGCTGAATT AACCAAGGAC TACAAGGAGG GGGACAAGAG CCTCCAGCTC 
CGACTGGAAG ATGAAACCAT TCAGGAGTAC CCCATCGATG TCCAAAACAA CCAACTTCCC 
TTCCTGCGGA ATCCAGATAT CCTGGTGGGA GAAAATGACC TAACTGCCCT TAGCTATCTC 
CATGAGCCTG CAGTTTTGCA TAATTTAAAG GTCCGCTTCC TGGAGTCCAA CCATATCTAC 
ACTTACTGTG GGATTGTGCT TGTTGCCATT AATCCTTATG ATCAGTTGCC AATCTACGGT 
CAAGATGTCA TCTATGCCTA CAGTGGCCAG AACATGGGGG ATATGGACCC CCACATCTTT 
GCTGTGGCAG AGGAAGCCTA CAAGCAGATG GCCAGAGATG AGAAGAACCA GTCCATCATA 
GTCAGTGGGG AGTCTGGAGC TGGCAAGACG GTGTCGGCCA AGTATGCCAT GCGCTACTTC 
ACCACGGTCA GTGGCTCAGC CAGTGACACC AACATCGAAG AGAAAGTCCT GGCGTCCAGT 
CCCATCATGG AGGCCATCGG GAATGCTAAA ACCACCCGCA ACGACAACAG CAGCCGTTTC 
GGGAAGTACA TTCAGATCGG CTTTGACAAG AGGTACCGCA TCATCGGGGC CAACATGAGG 
ACCTACCTGC TGGAGAAGTC CAGGGTGGTC TTTCAGGCAG ACGACGAGAG GAATTACCAC 
ATCTTTTACC AGCTCTGTGC GGCTGCCAGT CTTCCGGAAT TTAAGGAGCT GGCACTAACC 
TGTGCTGAGG ACTTCTTCTA CACGTCACAG GGAGGAAACA CAGTCATCGA GGGTGTGGAT 
GATGCTGAGG ACTTTGAGAA GACTCGACAA GCCTTCACGC TCGTTGGAGT GAGAGAGTCC 
CATCAGATAA GCATTTTTAA GATAATTGCT TCCATCCTGC ACCTCGGAAA TGTGGAGATT 
CAAGCCGAGC GCGATGGTGA ATCCTGTAGA GTATCACCTG AGGATGAACA CCTAAGCGAC 
TTCTGCCGCC TGCTGGGCGT GGAGCTCAGT CAGATGGAGC ACTGGCTGTG CCATCGCAAG 
CTGGTCACCA CCTCGGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAGGT GGTCAACGCG 
CGCGACGCCC TGGCCAAGCA CATCTATGCC CAGCTGTTCC ACTGGATCGT GGAGCACGTC 
AACAAGGCCC TGCACACCTC CCTCAAGCAG CACTCCTTCA TTGGAGTCCT GGACATCTAC 
GGGTTTGAGA CGTTTGAGGT GAACAGCTTC GAGCAGTTCT GTATCAACTA CGCCAATGAG 
AAGCTCCAGC AGCAGTTCAA CTCACACGTA TTCAAGCTGG AGCAGGAGGA GTACATGAAG 
GAGCAGATCC CGTGGACCCT GATTGATTTC TATGATAACC AGCCTTGTAT CGACCTCATA 
GAAGCTAAGC TGGGCATCTT GGACCTGTTG GATGAAGAAT GTAAGGTCCC CAAAGGAACC 
GACCAGAACT GGACTCAGAA GCTCTATGAC AGGCACTCTG GCAGCCAACA CTTCCAGAAA 
CCCCGCATGT CCAACAAGGC CTTCATTGTC ATCCACTTTG CAGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTGGAGAA AAACAGAGAT ACCGTGTACG AAGAACAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCGCT GGTGGCTGAC TTGTTCCATG ATGGCAAAGA CTCTGCTCCC 
ACCGCCACCG CTTCTTCCAA GATCAACATT CGCTCCTCCA GACCCCCTGT GAAAGCCTCC 
AACAAGGAGC ACAAGAAAAC CGTGGGTTAC CAGTTCCGCT CCTCCCTGCA TCTGCTCATG 
GAGACACTGA ACGCCACGAC ACCGCACTAT GTCCGCTGCA TCAAGCCCAA CGACGAGAAG 
CTGCCCTTCC ATTTTAACCC AAAGAGAGCA GTGCAGCAGC TCCGAGCCTG CGGCGTATTG 
GAGACCATCC GGATCAGCGC GGCTGGCTAC CCATCCAGGT GGTCCTACCA TGACTTCTTC 
AACCGGTATC GGGTGCTGGT CAAGAAGAGA GACCTTGCCA ACTCAGACAA AAAGGCCATC 
TGCAGGTCTG TGCTGGAGAG CCTCATCAAG GATCCTGACA AGTTCCAGTT TGGCCGCACC 
AAGATCTTCT TCCGGGCCGG CCAGGTGGCC TACCTGGAGA AGCTTCGGGC TGACAAGTTC 
CGGGCGGCCA CCATCATGAT CCAGAAAGCC GTGCGGGGCT GGCTGCAGAA GGTGAAGTAC 
CGCAGGTTGA AGGGAGCCAC CTTAATTCTG CAGAGGTACT GCCGAGGATA CCTGGCCCGC 
AGACTGGCCA AGCATCTGCG GAGGACCCGG GCGGCCGTGG TGCTCCAGAA GCAATACCGC 
ATGCAGAGGG CCCGCCGGGC CTACCAGAGG GTCCGCAGGG CCGCCGTCGT CATCCAGGCC 
TTTGCTCGGG GCATGTTTGT GCGGAGAATC TACCACCAGG TCCTCAGGGA GCACAAGGCC 
ACCATCATCC AGAAGCACGT GCGGGGCTGG ATGGCTCGCA GGCGCTTCCA GCGGCTGCGG 
GGTGCGGCCG TTGTCATCCA GTGCGGCTTC CGGAGGCTCA AGGCCAAGCA GGCGCTGAAG 
GCCCTCAGGA TCGAGGCGCG CTCCGCGGAG CACCTGAAGC GCCTCAACGT GGGCATGGAG 
AACAAGATTG TCCAGCTGCA GCGGAAGATC GATGACCAGA ACAAAGAGCT CAAGACGCTG 
TCGGAGCAGC TGTCAGCAAT CACCTCCACA CACACCATGG AAGTGGAGAA GCTGAAGAAG 
GAGGTGGCCT GCTACCAGCA GAGCCAGGGT GAGGACAGAG GCCCGCAGTT GCAGGAGGAG 
GTGGAGAGCC TGCGGACCGA GCTGCAGAGG GCCCACTCAG AGCGCAAGGT CCTGGAGGAC 
ACCCACACCC GGGAGAAGGA TGAGCTGAGG AAGCGAGTTG CAGACCTGGA ACAAGAAAAT 
GCACTCTTGA AAAATGAGAA GGAGCAGCTT AACAACCAGA TCCTGTGCCA GTCTAAAGAT 
GAGTTTGCCC AGAACTCTGT CAAGGAAAAC CTCATGATGA AGAAGGAGCT GGAGGAGGAG 
CGCTCCCGTT ATCAGAACCT CGTGAAGGAG TATTCCCGGC TGGAGCAGAG ATACGACAAC 
CTTCGGGATG AGATGACCAT CCTAAAGCAA ACCCCAGGCC ACCGGAGGAA CCCATCAAAC 
CAAAGCAGCT TAGAATCTGA CTCCAATTAC CCGTCCATCT CCACGTCTGA GGTGGGAGAC 
ACAGAGGACA CCCTCCAGCA GGTGGAGGAA ATTGGCCTGG AGAAGGCAGC CATGGACATG 
ACCGTCTTCC TGAAGCTGCA GAAGAGGGTG CGGGAGCTGG AGCAGGAGAG GAAGAAGCTG 
CAGGTGCAGC TGGAGAAGAA AGAACAGCAG GACGGCAAGA GAGTCCCGGC GGACCAACCA 
AGCAATGACT CAGATTTGGA CCAGGATGCA GATCTAGCCT ACAACAGTCT GAAGAGGCAG 
GAGCTGGAGT CGGAGAACAA GAAGCTGAAG AATGAGCTGA ACGAGCTGAG GAAAGCCGTG 
GCCGACCAAG CCTCGGAGCA CAGCCCGGCT GCCGCCGGCA CCCCCGACAG CTACAGCCTC 
CTGCTGAACC AGCTCCGGGT GGCCAACGAG GAGCTGGAGG TGCGCAAGGA GGAAGTTCTC 
ATCCTGCGGA CCCAGATCGT GAGCGCCGAC CAGCGCCGCC TGGCGGGCAA GGGCTCGGAG 
CCAAACATCA ATGCCCGAAC CAGTTGGCCG AACAGCGAGA AGCACGTGGA TCAGGAAGAT 
GCCATCGAGG CCTATCACGG GGTCTGCCAG ACTAACAGCA AGACTGAGGA TTGGGGCTAC 
TTGAATGAAG ATGGAGAGCT CGGCTTGGCC TACCAAGGCC TAAAGCAAGT TGCCAGGTTG 
CTGGAGGCAC AGCTGCAGGC CCAGAGCCGC GAGCACGAGG AGGAGGTGGA CAATCTCAAG 
GCCCAGCTGG AGGCCCTGAA GGAGGAGATG GACAGACAGC AGCAGACCTT CTGCCAGACC 
CTGCTGCTCT CCCCAGAGGC CCAGGTGGAG TTCGGCATCC AGCAGGAGAT GTCTCGGCTC 
ACCAACGAGA ACCTGGACCT TAAAGAGTTA GTTGAGAAGC TGGAGAAGAA CGAGAGGAAG 
CTCAAAAAGC AACTGAAGAT TTACATGAAG AAGGTCCAGG ACCTGGAAGC TGCCCAAGCC 
TTGGCCCAGA GCGAGAGGAA GCGGCACGAG CTCAACAGGC AGGTCACCGT GCAGCGGAAG 
GAGAAGGACT TCCAAGGCAT GCTGGAGTAC CACAAGGAGG ACGAGGCCCT GCTCATTCGG 
AATCTGGTGA CAGAGTTGAA ACCGCAGGTG CTGGCGGGCG CGGTGCCCTG CCTCCCAGCC 
TACATCCTCT ACATGTGCCT CCGGCACGCG GACTACGTGA ACGACGACCT CAAGGTGCAC 
TCCCTGCTGA CCTCCACCAT CAACGGCATC AAGAAGGTCC TCAAGAAGCA CAATGAGGAC 
TTTGAGATGA CGTCGTTCTG GTTGTCCAAC ACCTGCCGCC TCCTGCACTG CTTGAAGCAG 
TACAGCGGGG ACGAGGGCTT CATGACTCAG AACACTGCAA AGCAGAATGA GCACTGTCTT 
AAGAACTTTG ACCTCACTGA ATACCGCCAG GTGCTGAGCG ATCTTTCCAT TCAGATCTAC 
CAGCAGCTCA TTAAAATCGC CGAGGGCCTC TTGCAGCCTA TGATAGTGTC GGCCATGTTG 
GAGAACGAGA GCATTCAGGG TCTGTCTGGG GTGAAGCCCA CGGGCTACCG GAAGCGCACC 
TCCAGCATGC CGGAAGGAGA CAACTCGTAC TGCCTGGAAG CCATCATCCG CCAGATGAAC 
TCCTTCCACA CGGTTATGTG CGACCAGGGC CTGGACCCCG AGATCATCCT GCAGGTGTTC 
AGACAGCTCT TCTACATGAT CAACGCGGTG ACGCTCAACA ACCTGCTTCT GCGGAAGGAC 
GTCTGCTCCT GGAGCACAGG CATGCAGCTC AGGTACAACA TTAGTCAGCT GGAGGAATGG 
CTTCGGGGAA GAAACCTGCA CCAGAGCGGA GCTGTTGAGA CCATGGAACC CCTGATTCAG 
GCTGCCCAGC TCCTGCAGTT AAAGAAGAAA AGCCCCGAGG ATGCTGAGGC CATCTGCTCC 
CTGTGCACCG CCCTCAGCAC CCAGCAGATT GTCAAAATTT TGAACCTTTA TACCCCTCTG 
AATGAATTTG AAGAACGGGT GACAGTGGCT TTTATACGAA CAATCCAGGC ACAACTGCAA 
GATCGGAATG ACCCTCAGCA GCTGCTATTA GACTTCAAGC ACATGTTTCC TGTTTTGTTC 
CCGTTTAATC CATCTTCTCT GACCATGGAC TCAATCCACA TCCCAGCATG TCTCAATCTG 
GAGTTCCTCA ACGAAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324662.1
CDS	197..5737
Translation	MSVSELYSQYTRVWIPDPDEVWRSAELTKDYKEGDKSLQLRLEDETIQEYPIDVQNNQLPFLRNPDILVGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYDQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFTTVSGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYRIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTSQGGNTVIEGVDDAEDFEKTRQAFTLVGVRESHQISIFKIIASILHLGNVEIQAERDGESCRVSPEDEHLSDFCRLLGVELSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARDALAKHIYAQLFHWIVEHVNKALHTSLKQHSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWTQKLYDRHSGSQHFQKPRMSNKAFIVIHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDGKDSAPTATASSKINIRSSRPPVKASNKEHKKTVGYQFRSSLHLLMETLNATTPHYVRCIKPNDEKLPFHFNPKRAVQQLRACGVLETIRISAAGYPSRWSYHDFFNRYRVLVKKRDLANSDKKAICRSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRAATIMIQKAVRGWLQKVKYRRLKGATLILQRYCRGYLARRLAKHLRRTRAAVVLQKQYRMQRARRAYQRVRRAAVVIQAFARGMFVRRIYHQVLREHKATIIQKHVRGWMARRRFQRLRGAAVVIQCGFRRLKAKQALKALRIEARSAEHLKRLNVGMENKIVQLQRKIDDQNKELKTLSEQLSAITSTHTMEVEKLKKEVACYQQSQGEDRGPQLQEEVESLRTELQRAHSERKVLEDTHTREKDELRKRVADLEQENALLKNEKEQLNNQILCQSKDEFAQNSVKENLMMKKELEEERSRYQNLVKEYSRLEQRYDNLRDEMTILKQTPGHRRNPSNQSSLESDSNYPSISTSEVGDTEDTLQQVEEIGLEKAAMDMTVFLKLQKRVRELEQERKKLQVQLEKKEQQDGKRVPADQPSNDSDLDQDADLAYNSLKRQELESENKKLKNELNELRKAVADQASEHSPAAAGTPDSYSLLLNQLRVANEELEVRKEEVLILRTQIVSADQRRLAGKGSEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNSKTEDWGYLNEDGELGLAYQGLKQVARLLEAQLQAQSREHEEEVDNLKAQLEALKEEMDRQQQTFCQTLLLSPEAQVEFGIQQEMSRLTNENLDLKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSERKRHELNRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTELKPQVLAGAVPCLPAYILYMCLRHADYVNDDLKVHSLLTSTINGIKKVLKKHNEDFEMTSFWLSNTCRLLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGLLQPMIVSAMLENESIQGLSGVKPTGYRKRTSSMPEGDNSYCLEAIIRQMNSFHTVMCDQGLDPEIILQVFRQLFYMINAVTLNNLLLRKDVCSWSTGMQLRYNISQLEEWLRGRNLHQSGAVETMEPLIQAAQLLQLKKKSPEDAEAICSLCTALSTQQIVKILNLYTPLNEFEERVTVAFIRTIQAQLQDRNDPQQLLLDFKHMFPVLFPFNPSSLTMDSIHIPACLNLEFLNEV

Target ORF information:

RefSeq Version	XM_015469176.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VB (MYO5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469176.1

ORF Insert Sequence:

ATGTCGGTCA GCGAGCTCTA CAGCCAGTAC ACAAGGGTCT GGATCCCTGA CCCGGATGAG 
GTGTGGCGCT CCGCTGAATT AACCAAGGAC TACAAGGAGG GGGACAAGAG CCTCCAGCTC 
CGACTGGAAG ATGAAACCAT TCAGGAGTAC CCCATCGATG TCCAAAACAA CCAACTTCCC 
TTCCTGCGGA ATCCAGATAT CCTGGTGGGA GAAAATGACC TAACTGCCCT TAGCTATCTC 
CATGAGCCTG CAGTTTTGCA TAATTTAAAG GTCCGCTTCC TGGAGTCCAA CCATATCTAC 
ACTTACTGTG GGATTGTGCT TGTTGCCATT AATCCTTATG ATCAGTTGCC AATCTACGGT 
CAAGATGTCA TCTATGCCTA CAGTGGCCAG AACATGGGGG ATATGGACCC CCACATCTTT 
GCTGTGGCAG AGGAAGCCTA CAAGCAGATG GCCAGAGATG AGAAGAACCA GTCCATCATA 
GTCAGTGGGG AGTCTGGAGC TGGCAAGACG GTGTCGGCCA AGTATGCCAT GCGCTACTTC 
ACCACGGTCA GTGGCTCAGC CAGTGACACC AACATCGAAG AGAAAGTCCT GGCGTCCAGT 
CCCATCATGG AGGCCATCGG GAATGCTAAA ACCACCCGCA ACGACAACAG CAGCCGTTTC 
GGGAAGTACA TTCAGATCGG CTTTGACAAG AGGTACCGCA TCATCGGGGC CAACATGAGG 
ACCTACCTGC TGGAGAAGTC CAGGGTGGTC TTTCAGGCAG ACGACGAGAG GAATTACCAC 
ATCTTTTACC AGCTCTGTGC GGCTGCCAGT CTTCCGGAAT TTAAGGAGCT GGCACTAACC 
TGTGCTGAGG ACTTCTTCTA CACGTCACAG GGAGGAAACA CAGTCATCGA GGGTGTGGAT 
GATGCTGAGG ACTTTGAGAA GACTCGACAA GCCTTCACGC TCGTTGGAGT GAGAGAGTCC 
CATCAGATAA GCATTTTTAA GATAATTGCT TCCATCCTGC ACCTCGGAAA TGTGGAGATT 
CAAGCCGAGC GCGATGGTGA ATCCTGTAGA GTATCACCTG AGGATGAACA CCTAAGCGAC 
TTCTGCCGCC TGCTGGGCGT GGAGCTCAGT CAGATGGAGC ACTGGCTGTG CCATCGCAAG 
CTGGTCACCA CCTCGGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAGGT GGTCAACGCG 
CGCGACGCCC TGGCCAAGCA CATCTATGCC CAGCTGTTCC ACTGGATCGT GGAGCACGTC 
AACAAGGCCC TGCACACCTC CCTCAAGCAG CACTCCTTCA TTGGAGTCCT GGACATCTAC 
GGGTTTGAGA CGTTTGAGGT GAACAGCTTC GAGCAGTTCT GTATCAACTA CGCCAATGAG 
AAGCTCCAGC AGCAGTTCAA CTCACACGTA TTCAAGCTGG AGCAGGAGGA GTACATGAAG 
GAGCAGATCC CGTGGACCCT GATTGATTTC TATGATAACC AGCCTTGTAT CGACCTCATA 
GAAGCTAAGC TGGGCATCTT GGACCTGTTG GATGAAGAAT GTAAGGTCCC CAAAGGAACC 
GACCAGAACT GGACTCAGAA GCTCTATGAC AGGCACTCTG GCAGCCAACA CTTCCAGAAA 
CCCCGCATGT CCAACAAGGC CTTCATTGTC ATCCACTTTG CAGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTGGAGAA AAACAGAGAT ACCGTGTACG AAGAACAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCGCT GGTGGCTGAC TTGTTCCATG ATGGCAAAGA CTCTGCTCCC 
ACCGCCACCG CTTCTTCCAA GATCAACATT CGCTCCTCCA GACCCCCTGT GAAAGCCTCC 
AACAAGGAGC ACAAGAAAAC CGTGGGTTAC CAGTTCCGCT CCTCCCTGCA TCTGCTCATG 
GAGACACTGA ACGCCACGAC ACCGCACTAT GTCCGCTGCA TCAAGCCCAA CGACGAGAAG 
CTGCCCTTCC ATTTTAACCC AAAGAGAGCA GTGCAGCAGC TCCGAGCCTG CGGCGTATTG 
GAGACCATCC GGATCAGCGC GGCTGGCTAC CCATCCAGGT GGTCCTACCA TGACTTCTTC 
AACCGGTATC GGGTGCTGGT CAAGAAGAGA GACCTTGCCA ACTCAGACAA AAAGGCCATC 
TGCAGGTCTG TGCTGGAGAG CCTCATCAAG GATCCTGACA AGTTCCAGTT TGGCCGCACC 
AAGATCTTCT TCCGGGCCGG CCAGGTGGCC TACCTGGAGA AGCTTCGGGC TGACAAGTTC 
CGGGCGGCCA CCATCATGAT CCAGAAAGCC GTGCGGGGCT GGCTGCAGAA GGTGAAGTAC 
CGCAGGTTGA AGGGAGCCAC CTTAATTCTG CAGAGGTACT GCCGAGGATA CCTGGCCCGC 
AGACTGGCCA AGCATCTGCG GAGGACCCGG GCGGCCGTGG TGCTCCAGAA GCAATACCGC 
ATGCAGAGGG CCCGCCGGGC CTACCAGAGG GTCCGCAGGG CCGCCGTCGT CATCCAGGCC 
TTTGCTCGGG GCATGTTTGT GCGGAGAATC TACCACCAGG TCCTCAGGGA GCACAAGGCC 
ACCATCATCC AGAAGCACGT GCGGGGCTGG ATGGCTCGCA GGCGCTTCCA GCGGCTGCGG 
GGTGCGGCCG TTGTCATCCA GTGCGGCTTC CGGAGGCTCA AGGCCAAGCA GGCGCTGAAG 
GCCCTCAGGA TCGAGGCGCG CTCCGCGGAG CACCTGAAGC GCCTCAACGT GGGCATGGAG 
AACAAGATTG TCCAGCTGCA GCGGAAGATC GATGACCAGA ACAAAGAGCT CAAGACGCTG 
TCGGAGCAGC TGTCAGCAAT CACCTCCACA CACACCATGG AAGTGGAGAA GCTGAAGAAG 
GAGGTGGCCT GCTACCAGCA GAGCCAGGGT GAGGACAGAG GCCCGCAGTT GCAGGAGGAG 
GTGGAGAGCC TGCGGACCGA GCTGCAGAGG GCCCACTCAG AGCGCAAGGT CCTGGAGGAC 
ACCCACACCC GGGAGAAGGA TGAGCTGAGG AAGCGAGTTG CAGACCTGGA ACAAGAAAAT 
GCACTCTTGA AAAATGAGAA GGAGCAGCTT AACAACCAGA TCCTGTGCCA GTCTAAAGAT 
GAGTTTGCCC AGAACTCTGT CAAGGAAAAC CTCATGATGA AGAAGGAGCT GGAGGAGGAG 
CGCTCCCGTT ATCAGAACCT CGTGAAGGAG TATTCCCGGC TGGAGCAGAG ATACGACAAC 
CTTCGGGATG AGATGACCAT CCTAAAGCAA ACCCCAGGCC ACCGGAGGAA CCCATCAAAC 
CAAAGCAGCT TAGAATCTGA CTCCAATTAC CCGTCCATCT CCACGTCTGA GGTGGGAGAC 
ACAGAGGACA CCCTCCAGCA GGTGGAGGAA ATTGGCCTGG AGAAGGCAGC CATGGACATG 
ACCGTCTTCC TGAAGCTGCA GAAGAGGGTG CGGGAGCTGG AGCAGGAGAG GAAGAAGCTG 
CAGGTGCAGC TGGAGAAGAA AGAACAGCAG GACGGCAAGA GAGTCCCGGC GGACCAACCA 
AGCAATGACT CAGATTTGGA CCAGGATGCA GATCTAGCCT ACAACAGTCT GAAGAGGCAG 
GAGCTGGAGT CGGAGAACAA GAAGCTGAAG AATGAGCTGA ACGAGCTGAG GAAAGCCGTG 
GCCGACCAAG CCTCGGAGCA CAGCCCGGCT GCCGCCGGCA CCCCCGACAG CTACAGCCTC 
CTGCTGAACC AGCTCCGGGT GGCCAACGAG GAGCTGGAGG TGCGCAAGGA GGAAGTTCTC 
ATCCTGCGGA CCCAGATCGT GAGCGCCGAC CAGCGCCGCC TGGCGGGCAA GGGCTCGGAG 
CCAAACATCA ATGCCCGAAC CAGTTGGCCG AACAGCGAGA AGCACGTGGA TCAGGAAGAT 
GCCATCGAGG CCTATCACGG GGTCTGCCAG ACTAACAGCA AGACTGAGGA TTGGGGCTAC 
TTGAATGAAG ATGGAGAGCT CGGCTTGGCC TACCAAGGCC TAAAGCAAGT TGCCAGGTTG 
CTGGAGGCAC AGCTGCAGGC CCAGAGCCGC GAGCACGAGG AGGAGGTGGA CAATCTCAAG 
GCCCAGCTGG AGGCCCTGAA GGAGGAGATG GACAGACAGC AGCAGACCTT CTGCCAGACC 
CTGCTGCTCT CCCCAGAGGC CCAGGTGGAG TTCGGCATCC AGCAGGAGAT GTCTCGGCTC 
ACCAACGAGA ACCTGGACCT TAAAGAGTTA GTTGAGAAGC TGGAGAAGAA CGAGAGGAAG 
CTCAAAAAGC AACTGAAGAT TTACATGAAG AAGGTCCAGG ACCTGGAAGC TGCCCAAGCC 
TTGGCCCAGA GCGAGAGGAA GCGGCACGAG CTCAACAGGC AGGTCACCGT GCAGCGGAAG 
GAGAAGGACT TCCAAGGCAT GCTGGAGTAC CACAAGGAGG ACGAGGCCCT GCTCATTCGG 
AATCTGGTGA CAGAGTTGAA ACCGCAGGTG CTGGCGGGCG CGGTGCCCTG CCTCCCAGCC 
TACATCCTCT ACATGTGCCT CCGGCACGCG GACTACGTGA ACGACGACCT CAAGGTGCAC 
TCCCTGCTGA CCTCCACCAT CAACGGCATC AAGAAGGTCC TCAAGAAGCA CAATGAGGAC 
TTTGAGATGA CGTCGTTCTG GTTGTCCAAC ACCTGCCGCC TCCTGCACTG CTTGAAGCAG 
TACAGCGGGG ACGAGGGCTT CATGACTCAG AACACTGCAA AGCAGAATGA GCACTGTCTT 
AAGAACTTTG ACCTCACTGA ATACCGCCAG GTGCTGAGCG ATCTTTCCAT TCAGATCTAC 
CAGCAGCTCA TTAAAATCGC CGAGGGCCTC TTGCAGCCTA TGATAGTGTC GGCCATGTTG 
GAGAACGAGA GCATTCAGGG TCTGTCTGGG GTGAAGCCCA CGGGCTACCG GAAGCGCACC 
TCCAGCATGC CGGAAGGAGA CAACTCGTAC TGCCTGGAAG CCATCATCCG CCAGATGAAC 
TCCTTCCACA CGGTTATGTG CGACCAGGGC CTGGACCCCG AGATCATCCT GCAGGTGTTC 
AGACAGCTCT TCTACATGAT CAACGCGGTG ACGCTCAACA ACCTGCTTCT GCGGAAGGAC 
GTCTGCTCCT GGAGCACAGG CATGCAGCTC AGGTACAACA TTAGTCAGCT GGAGGAATGG 
CTTCGGGGAA GAAACCTGCA CCAGAGCGGA GCTGTTGAGA CCATGGAACC CCTGATTCAG 
GCTGCCCAGC TCCTGCAGTT AAAGAAGAAA AGCCCCGAGG ATGCTGAGGC CATCTGCTCC 
CTGTGCACCG CCCTCAGCAC CCAGCAGATT GTCAAAATTT TGAACCTTTA TACCCCTCTG 
AATGAATTTG AAGAACGGGT GACAGTGGCT TTTATACGAA CAATCCAGGC ACAACTGCAA 
GATCGGAATG ACCCTCAGCA GCTGCTATTA GACTTCAAGC ACATGTTTCC TGTTTTGTTC 
CCGTTTAATC CATCTTCTCT GACCATGGAC TCAATCCACA TCCCAGCATG TCTCAATCTG 
GAGTTCCTCA ACGAAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5B ( XM_002697821.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697867.2 Bos taurus myosin VB (MYO5B), transcript variant X1, mRNA.

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CloneID	OBa32664
Clone ID Related Accession (Same CDS sequence)	XM_002697821.5 , XM_015469176.1 , XM_002697821.4
Accession Version	XM_002697821.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Vb isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697821.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCA GCGAGCTCTA CAGCCAGTAC ACAAGGGTCT GGATCCCTGA CCCGGATGAG 
GTGTGGCGCT CCGCTGAATT AACCAAGGAC TACAAGGAGG GGGACAAGAG CCTCCAGCTC 
CGACTGGAAG ATGAAACCAT TCAGGAGTAC CCCATCGATG TCCAAAACAA CCAACTTCCC 
TTCCTGCGGA ATCCAGATAT CCTGGTGGGA GAAAATGACC TAACTGCCCT TAGCTATCTC 
CATGAGCCTG CAGTTTTGCA TAATTTAAAG GTCCGCTTCC TGGAGTCCAA CCATATCTAC 
ACTTACTGTG GGATTGTGCT TGTTGCCATT AATCCTTATG ATCAGTTGCC AATCTACGGT 
CAAGATGTCA TCTATGCCTA CAGTGGCCAG AACATGGGGG ATATGGACCC CCACATCTTT 
GCTGTGGCAG AGGAAGCCTA CAAGCAGATG GCCAGAGATG AGAAGAACCA GTCCATCATA 
GTCAGTGGGG AGTCTGGAGC TGGCAAGACG GTGTCGGCCA AGTATGCCAT GCGCTACTTC 
ACCACGGTCA GTGGCTCAGC CAGTGACACC AACATCGAAG AGAAAGTCCT GGCGTCCAGT 
CCCATCATGG AGGCCATCGG GAATGCTAAA ACCACCCGCA ACGACAACAG CAGCCGTTTC 
GGGAAGTACA TTCAGATCGG CTTTGACAAG AGGTACCGCA TCATCGGGGC CAACATGAGG 
ACCTACCTGC TGGAGAAGTC CAGGGTGGTC TTTCAGGCAG ACGACGAGAG GAATTACCAC 
ATCTTTTACC AGCTCTGTGC GGCTGCCAGT CTTCCGGAAT TTAAGGAGCT GGCACTAACC 
TGTGCTGAGG ACTTCTTCTA CACGTCACAG GGAGGAAACA CAGTCATCGA GGGTGTGGAT 
GATGCTGAGG ACTTTGAGAA GACTCGACAA GCCTTCACGC TCGTTGGAGT GAGAGAGTCC 
CATCAGATAA GCATTTTTAA GATAATTGCT TCCATCCTGC ACCTCGGAAA TGTGGAGATT 
CAAGCCGAGC GCGATGGTGA ATCCTGTAGA GTATCACCTG AGGATGAACA CCTAAGCGAC 
TTCTGCCGCC TGCTGGGCGT GGAGCTCAGT CAGATGGAGC ACTGGCTGTG CCATCGCAAG 
CTGGTCACCA CCTCGGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAGGT GGTCAACGCG 
CGCGACGCCC TGGCCAAGCA CATCTATGCC CAGCTGTTCC ACTGGATCGT GGAGCACGTC 
AACAAGGCCC TGCACACCTC CCTCAAGCAG CACTCCTTCA TTGGAGTCCT GGACATCTAC 
GGGTTTGAGA CGTTTGAGGT GAACAGCTTC GAGCAGTTCT GTATCAACTA CGCCAATGAG 
AAGCTCCAGC AGCAGTTCAA CTCACACGTA TTCAAGCTGG AGCAGGAGGA GTACATGAAG 
GAGCAGATCC CGTGGACCCT GATTGATTTC TATGATAACC AGCCTTGTAT CGACCTCATA 
GAAGCTAAGC TGGGCATCTT GGACCTGTTG GATGAAGAAT GTAAGGTCCC CAAAGGAACC 
GACCAGAACT GGACTCAGAA GCTCTATGAC AGGCACTCTG GCAGCCAACA CTTCCAGAAA 
CCCCGCATGT CCAACAAGGC CTTCATTGTC ATCCACTTTG CAGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTGGAGAA AAACAGAGAT ACCGTGTACG AAGAACAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCGCT GGTGGCTGAC TTGTTCCATG ATGGCAAAGA CTCTGCTCCC 
ACCGCCACCG CTTCTTCCAA GATCAACATT CGCTCCTCCA GACCCCCTGT GAAAGCCTCC 
AACAAGGAGC ACAAGAAAAC CGTGGGTTAC CAGTTCCGCT CCTCCCTGCA TCTGCTCATG 
GAGACACTGA ACGCCACGAC ACCGCACTAT GTCCGCTGCA TCAAGCCCAA CGACGAGAAG 
CTGCCCTTCC ATTTTAACCC AAAGAGAGCA GTGCAGCAGC TCCGAGCCTG CGGCGTATTG 
GAGACCATCC GGATCAGCGC GGCTGGCTAC CCATCCAGGT GGTCCTACCA TGACTTCTTC 
AACCGGTATC GGGTGCTGGT CAAGAAGAGA GACCTTGCCA ACTCAGACAA AAAGGCCATC 
TGCAGGTCTG TGCTGGAGAG CCTCATCAAG GATCCTGACA AGTTCCAGTT TGGCCGCACC 
AAGATCTTCT TCCGGGCCGG CCAGGTGGCC TACCTGGAGA AGCTTCGGGC TGACAAGTTC 
CGGGCGGCCA CCATCATGAT CCAGAAAGCC GTGCGGGGCT GGCTGCAGAA GGTGAAGTAC 
CGCAGGTTGA AGGGAGCCAC CTTAATTCTG CAGAGGTACT GCCGAGGATA CCTGGCCCGC 
AGACTGGCCA AGCATCTGCG GAGGACCCGG GCGGCCGTGG TGCTCCAGAA GCAATACCGC 
ATGCAGAGGG CCCGCCGGGC CTACCAGAGG GTCCGCAGGG CCGCCGTCGT CATCCAGGCC 
TTTGCTCGGG GCATGTTTGT GCGGAGAATC TACCACCAGG TCCTCAGGGA GCACAAGGCC 
ACCATCATCC AGAAGCACGT GCGGGGCTGG ATGGCTCGCA GGCGCTTCCA GCGGCTGCGG 
GGTGCGGCCG TTGTCATCCA GTGCGGCTTC CGGAGGCTCA AGGCCAAGCA GGCGCTGAAG 
GCCCTCAGGA TCGAGGCGCG CTCCGCGGAG CACCTGAAGC GCCTCAACGT GGGCATGGAG 
AACAAGATTG TCCAGCTGCA GCGGAAGATC GATGACCAGA ACAAAGAGCT CAAGACGCTG 
TCGGAGCAGC TGTCAGCAAT CACCTCCACA CACACCATGG AAGTGGAGAA GCTGAAGAAG 
GAGGTGGCCT GCTACCAGCA GAGCCAGGGT GAGGACAGAG GCCCGCAGTT GCAGGAGGAG 
GTGGAGAGCC TGCGGACCGA GCTGCAGAGG GCCCACTCAG AGCGCAAGGT CCTGGAGGAC 
ACCCACACCC GGGAGAAGGA TGAGCTGAGG AAGCGAGTTG CAGACCTGGA ACAAGAAAAT 
GCACTCTTGA AAAATGAGAA GGAGCAGCTT AACAACCAGA TCCTGTGCCA GTCTAAAGAT 
GAGTTTGCCC AGAACTCTGT CAAGGAAAAC CTCATGATGA AGAAGGAGCT GGAGGAGGAG 
CGCTCCCGTT ATCAGAACCT CGTGAAGGAG TATTCCCGGC TGGAGCAGAG ATACGACAAC 
CTTCGGGATG AGATGACCAT CCTAAAGCAA ACCCCAGGCC ACCGGAGGAA CCCATCAAAC 
CAAAGCAGCT TAGAATCTGA CTCCAATTAC CCGTCCATCT CCACGTCTGA GGTGGGAGAC 
ACAGAGGACA CCCTCCAGCA GGTGGAGGAA ATTGGCCTGG AGAAGGCAGC CATGGACATG 
ACCGTCTTCC TGAAGCTGCA GAAGAGGGTG CGGGAGCTGG AGCAGGAGAG GAAGAAGCTG 
CAGGTGCAGC TGGAGAAGAA AGAACAGCAG GACGGCAAGA GAGTCCCGGC GGACCAACCA 
AGCAATGACT CAGATTTGGA CCAGGATGCA GATCTAGCCT ACAACAGTCT GAAGAGGCAG 
GAGCTGGAGT CGGAGAACAA GAAGCTGAAG AATGAGCTGA ACGAGCTGAG GAAAGCCGTG 
GCCGACCAAG CCTCGGAGCA CAGCCCGGCT GCCGCCGGCA CCCCCGACAG CTACAGCCTC 
CTGCTGAACC AGCTCCGGGT GGCCAACGAG GAGCTGGAGG TGCGCAAGGA GGAAGTTCTC 
ATCCTGCGGA CCCAGATCGT GAGCGCCGAC CAGCGCCGCC TGGCGGGCAA GGGCTCGGAG 
CCAAACATCA ATGCCCGAAC CAGTTGGCCG AACAGCGAGA AGCACGTGGA TCAGGAAGAT 
GCCATCGAGG CCTATCACGG GGTCTGCCAG ACTAACAGCA AGACTGAGGA TTGGGGCTAC 
TTGAATGAAG ATGGAGAGCT CGGCTTGGCC TACCAAGGCC TAAAGCAAGT TGCCAGGTTG 
CTGGAGGCAC AGCTGCAGGC CCAGAGCCGC GAGCACGAGG AGGAGGTGGA CAATCTCAAG 
GCCCAGCTGG AGGCCCTGAA GGAGGAGATG GACAGACAGC AGCAGACCTT CTGCCAGACC 
CTGCTGCTCT CCCCAGAGGC CCAGGTGGAG TTCGGCATCC AGCAGGAGAT GTCTCGGCTC 
ACCAACGAGA ACCTGGACCT TAAAGAGTTA GTTGAGAAGC TGGAGAAGAA CGAGAGGAAG 
CTCAAAAAGC AACTGAAGAT TTACATGAAG AAGGTCCAGG ACCTGGAAGC TGCCCAAGCC 
TTGGCCCAGA GCGAGAGGAA GCGGCACGAG CTCAACAGGC AGGTCACCGT GCAGCGGAAG 
GAGAAGGACT TCCAAGGCAT GCTGGAGTAC CACAAGGAGG ACGAGGCCCT GCTCATTCGG 
AATCTGGTGA CAGAGTTGAA ACCGCAGGTG CTGGCGGGCG CGGTGCCCTG CCTCCCAGCC 
TACATCCTCT ACATGTGCCT CCGGCACGCG GACTACGTGA ACGACGACCT CAAGGTGCAC 
TCCCTGCTGA CCTCCACCAT CAACGGCATC AAGAAGGTCC TCAAGAAGCA CAATGAGGAC 
TTTGAGATGA CGTCGTTCTG GTTGTCCAAC ACCTGCCGCC TCCTGCACTG CTTGAAGCAG 
TACAGCGGGG ACGAGGGCTT CATGACTCAG AACACTGCAA AGCAGAATGA GCACTGTCTT 
AAGAACTTTG ACCTCACTGA ATACCGCCAG GTGCTGAGCG ATCTTTCCAT TCAGATCTAC 
CAGCAGCTCA TTAAAATCGC CGAGGGCCTC TTGCAGCCTA TGATAGTGTC GGCCATGTTG 
GAGAACGAGA GCATTCAGGG TCTGTCTGGG GTGAAGCCCA CGGGCTACCG GAAGCGCACC 
TCCAGCATGC CGGAAGGAGA CAACTCGTAC TGCCTGGAAG CCATCATCCG CCAGATGAAC 
TCCTTCCACA CGGTTATGTG CGACCAGGGC CTGGACCCCG AGATCATCCT GCAGGTGTTC 
AGACAGCTCT TCTACATGAT CAACGCGGTG ACGCTCAACA ACCTGCTTCT GCGGAAGGAC 
GTCTGCTCCT GGAGCACAGG CATGCAGCTC AGGTACAACA TTAGTCAGCT GGAGGAATGG 
CTTCGGGGAA GAAACCTGCA CCAGAGCGGA GCTGTTGAGA CCATGGAACC CCTGATTCAG 
GCTGCCCAGC TCCTGCAGTT AAAGAAGAAA AGCCCCGAGG ATGCTGAGGC CATCTGCTCC 
CTGTGCACCG CCCTCAGCAC CCAGCAGATT GTCAAAATTT TGAACCTTTA TACCCCTCTG 
AATGAATTTG AAGAACGGGT GACAGTGGCT TTTATACGAA CAATCCAGGC ACAACTGCAA 
GATCGGAATG ACCCTCAGCA GCTGCTATTA GACTTCAAGC ACATGTTTCC TGTTTTGTTC 
CCGTTTAATC CATCTTCTCT GACCATGGAC TCAATCCACA TCCCAGCATG TCTCAATCTG 
GAGTTCCTCA ACGAAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697867.2
CDS	165..5705
Translation	MSVSELYSQYTRVWIPDPDEVWRSAELTKDYKEGDKSLQLRLEDETIQEYPIDVQNNQLPFLRNPDILVGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYDQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFTTVSGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYRIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTSQGGNTVIEGVDDAEDFEKTRQAFTLVGVRESHQISIFKIIASILHLGNVEIQAERDGESCRVSPEDEHLSDFCRLLGVELSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARDALAKHIYAQLFHWIVEHVNKALHTSLKQHSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWTQKLYDRHSGSQHFQKPRMSNKAFIVIHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDGKDSAPTATASSKINIRSSRPPVKASNKEHKKTVGYQFRSSLHLLMETLNATTPHYVRCIKPNDEKLPFHFNPKRAVQQLRACGVLETIRISAAGYPSRWSYHDFFNRYRVLVKKRDLANSDKKAICRSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRAATIMIQKAVRGWLQKVKYRRLKGATLILQRYCRGYLARRLAKHLRRTRAAVVLQKQYRMQRARRAYQRVRRAAVVIQAFARGMFVRRIYHQVLREHKATIIQKHVRGWMARRRFQRLRGAAVVIQCGFRRLKAKQALKALRIEARSAEHLKRLNVGMENKIVQLQRKIDDQNKELKTLSEQLSAITSTHTMEVEKLKKEVACYQQSQGEDRGPQLQEEVESLRTELQRAHSERKVLEDTHTREKDELRKRVADLEQENALLKNEKEQLNNQILCQSKDEFAQNSVKENLMMKKELEEERSRYQNLVKEYSRLEQRYDNLRDEMTILKQTPGHRRNPSNQSSLESDSNYPSISTSEVGDTEDTLQQVEEIGLEKAAMDMTVFLKLQKRVRELEQERKKLQVQLEKKEQQDGKRVPADQPSNDSDLDQDADLAYNSLKRQELESENKKLKNELNELRKAVADQASEHSPAAAGTPDSYSLLLNQLRVANEELEVRKEEVLILRTQIVSADQRRLAGKGSEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNSKTEDWGYLNEDGELGLAYQGLKQVARLLEAQLQAQSREHEEEVDNLKAQLEALKEEMDRQQQTFCQTLLLSPEAQVEFGIQQEMSRLTNENLDLKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSERKRHELNRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTELKPQVLAGAVPCLPAYILYMCLRHADYVNDDLKVHSLLTSTINGIKKVLKKHNEDFEMTSFWLSNTCRLLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGLLQPMIVSAMLENESIQGLSGVKPTGYRKRTSSMPEGDNSYCLEAIIRQMNSFHTVMCDQGLDPEIILQVFRQLFYMINAVTLNNLLLRKDVCSWSTGMQLRYNISQLEEWLRGRNLHQSGAVETMEPLIQAAQLLQLKKKSPEDAEAICSLCTALSTQQIVKILNLYTPLNEFEERVTVAFIRTIQAQLQDRNDPQQLLLDFKHMFPVLFPFNPSSLTMDSIHIPACLNLEFLNEV

Target ORF information:

RefSeq Version	XM_002697821.4
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VB (MYO5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697821.4

ORF Insert Sequence:

ATGTCGGTCA GCGAGCTCTA CAGCCAGTAC ACAAGGGTCT GGATCCCTGA CCCGGATGAG 
GTGTGGCGCT CCGCTGAATT AACCAAGGAC TACAAGGAGG GGGACAAGAG CCTCCAGCTC 
CGACTGGAAG ATGAAACCAT TCAGGAGTAC CCCATCGATG TCCAAAACAA CCAACTTCCC 
TTCCTGCGGA ATCCAGATAT CCTGGTGGGA GAAAATGACC TAACTGCCCT TAGCTATCTC 
CATGAGCCTG CAGTTTTGCA TAATTTAAAG GTCCGCTTCC TGGAGTCCAA CCATATCTAC 
ACTTACTGTG GGATTGTGCT TGTTGCCATT AATCCTTATG ATCAGTTGCC AATCTACGGT 
CAAGATGTCA TCTATGCCTA CAGTGGCCAG AACATGGGGG ATATGGACCC CCACATCTTT 
GCTGTGGCAG AGGAAGCCTA CAAGCAGATG GCCAGAGATG AGAAGAACCA GTCCATCATA 
GTCAGTGGGG AGTCTGGAGC TGGCAAGACG GTGTCGGCCA AGTATGCCAT GCGCTACTTC 
ACCACGGTCA GTGGCTCAGC CAGTGACACC AACATCGAAG AGAAAGTCCT GGCGTCCAGT 
CCCATCATGG AGGCCATCGG GAATGCTAAA ACCACCCGCA ACGACAACAG CAGCCGTTTC 
GGGAAGTACA TTCAGATCGG CTTTGACAAG AGGTACCGCA TCATCGGGGC CAACATGAGG 
ACCTACCTGC TGGAGAAGTC CAGGGTGGTC TTTCAGGCAG ACGACGAGAG GAATTACCAC 
ATCTTTTACC AGCTCTGTGC GGCTGCCAGT CTTCCGGAAT TTAAGGAGCT GGCACTAACC 
TGTGCTGAGG ACTTCTTCTA CACGTCACAG GGAGGAAACA CAGTCATCGA GGGTGTGGAT 
GATGCTGAGG ACTTTGAGAA GACTCGACAA GCCTTCACGC TCGTTGGAGT GAGAGAGTCC 
CATCAGATAA GCATTTTTAA GATAATTGCT TCCATCCTGC ACCTCGGAAA TGTGGAGATT 
CAAGCCGAGC GCGATGGTGA ATCCTGTAGA GTATCACCTG AGGATGAACA CCTAAGCGAC 
TTCTGCCGCC TGCTGGGCGT GGAGCTCAGT CAGATGGAGC ACTGGCTGTG CCATCGCAAG 
CTGGTCACCA CCTCGGAGAC CTACGTCAAG ACCATGTCCC TGCAGCAGGT GGTCAACGCG 
CGCGACGCCC TGGCCAAGCA CATCTATGCC CAGCTGTTCC ACTGGATCGT GGAGCACGTC 
AACAAGGCCC TGCACACCTC CCTCAAGCAG CACTCCTTCA TTGGAGTCCT GGACATCTAC 
GGGTTTGAGA CGTTTGAGGT GAACAGCTTC GAGCAGTTCT GTATCAACTA CGCCAATGAG 
AAGCTCCAGC AGCAGTTCAA CTCACACGTA TTCAAGCTGG AGCAGGAGGA GTACATGAAG 
GAGCAGATCC CGTGGACCCT GATTGATTTC TATGATAACC AGCCTTGTAT CGACCTCATA 
GAAGCTAAGC TGGGCATCTT GGACCTGTTG GATGAAGAAT GTAAGGTCCC CAAAGGAACC 
GACCAGAACT GGACTCAGAA GCTCTATGAC AGGCACTCTG GCAGCCAACA CTTCCAGAAA 
CCCCGCATGT CCAACAAGGC CTTCATTGTC ATCCACTTTG CAGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTGGAGAA AAACAGAGAT ACCGTGTACG AAGAACAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCGCT GGTGGCTGAC TTGTTCCATG ATGGCAAAGA CTCTGCTCCC 
ACCGCCACCG CTTCTTCCAA GATCAACATT CGCTCCTCCA GACCCCCTGT GAAAGCCTCC 
AACAAGGAGC ACAAGAAAAC CGTGGGTTAC CAGTTCCGCT CCTCCCTGCA TCTGCTCATG 
GAGACACTGA ACGCCACGAC ACCGCACTAT GTCCGCTGCA TCAAGCCCAA CGACGAGAAG 
CTGCCCTTCC ATTTTAACCC AAAGAGAGCA GTGCAGCAGC TCCGAGCCTG CGGCGTATTG 
GAGACCATCC GGATCAGCGC GGCTGGCTAC CCATCCAGGT GGTCCTACCA TGACTTCTTC 
AACCGGTATC GGGTGCTGGT CAAGAAGAGA GACCTTGCCA ACTCAGACAA AAAGGCCATC 
TGCAGGTCTG TGCTGGAGAG CCTCATCAAG GATCCTGACA AGTTCCAGTT TGGCCGCACC 
AAGATCTTCT TCCGGGCCGG CCAGGTGGCC TACCTGGAGA AGCTTCGGGC TGACAAGTTC 
CGGGCGGCCA CCATCATGAT CCAGAAAGCC GTGCGGGGCT GGCTGCAGAA GGTGAAGTAC 
CGCAGGTTGA AGGGAGCCAC CTTAATTCTG CAGAGGTACT GCCGAGGATA CCTGGCCCGC 
AGACTGGCCA AGCATCTGCG GAGGACCCGG GCGGCCGTGG TGCTCCAGAA GCAATACCGC 
ATGCAGAGGG CCCGCCGGGC CTACCAGAGG GTCCGCAGGG CCGCCGTCGT CATCCAGGCC 
TTTGCTCGGG GCATGTTTGT GCGGAGAATC TACCACCAGG TCCTCAGGGA GCACAAGGCC 
ACCATCATCC AGAAGCACGT GCGGGGCTGG ATGGCTCGCA GGCGCTTCCA GCGGCTGCGG 
GGTGCGGCCG TTGTCATCCA GTGCGGCTTC CGGAGGCTCA AGGCCAAGCA GGCGCTGAAG 
GCCCTCAGGA TCGAGGCGCG CTCCGCGGAG CACCTGAAGC GCCTCAACGT GGGCATGGAG 
AACAAGATTG TCCAGCTGCA GCGGAAGATC GATGACCAGA ACAAAGAGCT CAAGACGCTG 
TCGGAGCAGC TGTCAGCAAT CACCTCCACA CACACCATGG AAGTGGAGAA GCTGAAGAAG 
GAGGTGGCCT GCTACCAGCA GAGCCAGGGT GAGGACAGAG GCCCGCAGTT GCAGGAGGAG 
GTGGAGAGCC TGCGGACCGA GCTGCAGAGG GCCCACTCAG AGCGCAAGGT CCTGGAGGAC 
ACCCACACCC GGGAGAAGGA TGAGCTGAGG AAGCGAGTTG CAGACCTGGA ACAAGAAAAT 
GCACTCTTGA AAAATGAGAA GGAGCAGCTT AACAACCAGA TCCTGTGCCA GTCTAAAGAT 
GAGTTTGCCC AGAACTCTGT CAAGGAAAAC CTCATGATGA AGAAGGAGCT GGAGGAGGAG 
CGCTCCCGTT ATCAGAACCT CGTGAAGGAG TATTCCCGGC TGGAGCAGAG ATACGACAAC 
CTTCGGGATG AGATGACCAT CCTAAAGCAA ACCCCAGGCC ACCGGAGGAA CCCATCAAAC 
CAAAGCAGCT TAGAATCTGA CTCCAATTAC CCGTCCATCT CCACGTCTGA GGTGGGAGAC 
ACAGAGGACA CCCTCCAGCA GGTGGAGGAA ATTGGCCTGG AGAAGGCAGC CATGGACATG 
ACCGTCTTCC TGAAGCTGCA GAAGAGGGTG CGGGAGCTGG AGCAGGAGAG GAAGAAGCTG 
CAGGTGCAGC TGGAGAAGAA AGAACAGCAG GACGGCAAGA GAGTCCCGGC GGACCAACCA 
AGCAATGACT CAGATTTGGA CCAGGATGCA GATCTAGCCT ACAACAGTCT GAAGAGGCAG 
GAGCTGGAGT CGGAGAACAA GAAGCTGAAG AATGAGCTGA ACGAGCTGAG GAAAGCCGTG 
GCCGACCAAG CCTCGGAGCA CAGCCCGGCT GCCGCCGGCA CCCCCGACAG CTACAGCCTC 
CTGCTGAACC AGCTCCGGGT GGCCAACGAG GAGCTGGAGG TGCGCAAGGA GGAAGTTCTC 
ATCCTGCGGA CCCAGATCGT GAGCGCCGAC CAGCGCCGCC TGGCGGGCAA GGGCTCGGAG 
CCAAACATCA ATGCCCGAAC CAGTTGGCCG AACAGCGAGA AGCACGTGGA TCAGGAAGAT 
GCCATCGAGG CCTATCACGG GGTCTGCCAG ACTAACAGCA AGACTGAGGA TTGGGGCTAC 
TTGAATGAAG ATGGAGAGCT CGGCTTGGCC TACCAAGGCC TAAAGCAAGT TGCCAGGTTG 
CTGGAGGCAC AGCTGCAGGC CCAGAGCCGC GAGCACGAGG AGGAGGTGGA CAATCTCAAG 
GCCCAGCTGG AGGCCCTGAA GGAGGAGATG GACAGACAGC AGCAGACCTT CTGCCAGACC 
CTGCTGCTCT CCCCAGAGGC CCAGGTGGAG TTCGGCATCC AGCAGGAGAT GTCTCGGCTC 
ACCAACGAGA ACCTGGACCT TAAAGAGTTA GTTGAGAAGC TGGAGAAGAA CGAGAGGAAG 
CTCAAAAAGC AACTGAAGAT TTACATGAAG AAGGTCCAGG ACCTGGAAGC TGCCCAAGCC 
TTGGCCCAGA GCGAGAGGAA GCGGCACGAG CTCAACAGGC AGGTCACCGT GCAGCGGAAG 
GAGAAGGACT TCCAAGGCAT GCTGGAGTAC CACAAGGAGG ACGAGGCCCT GCTCATTCGG 
AATCTGGTGA CAGAGTTGAA ACCGCAGGTG CTGGCGGGCG CGGTGCCCTG CCTCCCAGCC 
TACATCCTCT ACATGTGCCT CCGGCACGCG GACTACGTGA ACGACGACCT CAAGGTGCAC 
TCCCTGCTGA CCTCCACCAT CAACGGCATC AAGAAGGTCC TCAAGAAGCA CAATGAGGAC 
TTTGAGATGA CGTCGTTCTG GTTGTCCAAC ACCTGCCGCC TCCTGCACTG CTTGAAGCAG 
TACAGCGGGG ACGAGGGCTT CATGACTCAG AACACTGCAA AGCAGAATGA GCACTGTCTT 
AAGAACTTTG ACCTCACTGA ATACCGCCAG GTGCTGAGCG ATCTTTCCAT TCAGATCTAC 
CAGCAGCTCA TTAAAATCGC CGAGGGCCTC TTGCAGCCTA TGATAGTGTC GGCCATGTTG 
GAGAACGAGA GCATTCAGGG TCTGTCTGGG GTGAAGCCCA CGGGCTACCG GAAGCGCACC 
TCCAGCATGC CGGAAGGAGA CAACTCGTAC TGCCTGGAAG CCATCATCCG CCAGATGAAC 
TCCTTCCACA CGGTTATGTG CGACCAGGGC CTGGACCCCG AGATCATCCT GCAGGTGTTC 
AGACAGCTCT TCTACATGAT CAACGCGGTG ACGCTCAACA ACCTGCTTCT GCGGAAGGAC 
GTCTGCTCCT GGAGCACAGG CATGCAGCTC AGGTACAACA TTAGTCAGCT GGAGGAATGG 
CTTCGGGGAA GAAACCTGCA CCAGAGCGGA GCTGTTGAGA CCATGGAACC CCTGATTCAG 
GCTGCCCAGC TCCTGCAGTT AAAGAAGAAA AGCCCCGAGG ATGCTGAGGC CATCTGCTCC 
CTGTGCACCG CCCTCAGCAC CCAGCAGATT GTCAAAATTT TGAACCTTTA TACCCCTCTG 
AATGAATTTG AAGAACGGGT GACAGTGGCT TTTATACGAA CAATCCAGGC ACAACTGCAA 
GATCGGAATG ACCCTCAGCA GCTGCTATTA GACTTCAAGC ACATGTTTCC TGTTTTGTTC 
CCGTTTAATC CATCTTCTCT GACCATGGAC TCAATCCACA TCCCAGCATG TCTCAATCTG 
GAGTTCCTCA ACGAAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5B ( XM_024984592.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840360.1 Bos taurus myosin VB (MYO5B), transcript variant X2, mRNA.

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CloneID	OBa48797
Clone ID Related Accession (Same CDS sequence)	XM_024984592.1
Accession Version	XM_024984592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	unconventional myosin-Vb isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GAGGTAGTAG CTTCCCTGTG CTGCATTGTA ACACACGCCA GTACACAAGG 
GTCTGGATCC CTGACCCGGA TGAGGTGTGG CGCTCCGCTG AATTAACCAA GGACTACAAG 
GAGGGGGACA AGAGCCTCCA GCTCCGACTG GAAGATGAAA CCATTCAGGA GTACCCCATC 
GATGTCCAAA ACAACCAACT TCCCTTCCTG CGGAATCCAG ATATCCTGGT GGGAGAAAAT 
GACCTAACTG CCCTTAGCTA TCTCCATGAG CCTGCAGTTT TGCATAATTT AAAGGTCCGC 
TTCCTGGAGT CCAACCATAT CTACACTTAC TGTGGGATTG TGCTTGTTGC CATTAATCCT 
TATGATCAGT TGCCAATCTA CGGTCAAGAT GTCATCTATG CCTACAGTGG CCAGAACATG 
GGGGATATGG ACCCCCACAT CTTTGCTGTG GCAGAGGAAG CCTACAAGCA GATGGCCAGA 
GATGAGAAGA ACCAGTCCAT CATAGTCAGT GGGGAGTCTG GAGCTGGCAA GACGGTGTCG 
GCCAAGTATG CCATGCGCTA CTTCACCACG GTCAGTGGCT CAGCCAGTGA CACCAACATC 
GAAGAGAAAG TCCTGGCGTC CAGTCCCATC ATGGAGGCCA TCGGGAATGC TAAAACCACC 
CGCAACGACA ACAGCAGCCG TTTCGGGAAG TACATTCAGA TCGGCTTTGA CAAGAGGTAC 
CGCATCATCG GGGCCAACAT GAGGACCTAC CTGCTGGAGA AGTCCAGGGT GGTCTTTCAG 
GCAGACGACG AGAGGAATTA CCACATCTTT TACCAGCTCT GTGCGGCTGC CAGTCTTCCG 
GAATTTAAGG AGCTGGCACT AACCTGTGCT GAGGACTTCT TCTACACGTC ACAGGGAGGA 
AACACAGTCA TCGAGGGTGT GGATGATGCT GAGGACTTTG AGAAGACTCG ACAAGCCTTC 
ACGCTCGTTG GAGTGAGAGA GTCCCATCAG ATAAGCATTT TTAAGATAAT TGCTTCCATC 
CTGCACCTCG GAAATGTGGA GATTCAAGCC GAGCGCGATG GTGAATCCTG TAGAGTATCA 
CCTGAGGATG AACACCTAAG CGACTTCTGC CGCCTGCTGG GCGTGGAGCT CAGTCAGATG 
GAGCACTGGC TGTGCCATCG CAAGCTGGTC ACCACCTCGG AGACCTACGT CAAGACCATG 
TCCCTGCAGC AGGTGGTCAA CGCGCGCGAC GCCCTGGCCA AGCACATCTA TGCCCAGCTG 
TTCCACTGGA TCGTGGAGCA CGTCAACAAG GCCCTGCACA CCTCCCTCAA GCAGCACTCC 
TTCATTGGAG TCCTGGACAT CTACGGGTTT GAGACGTTTG AGGTGAACAG CTTCGAGCAG 
TTCTGTATCA ACTACGCCAA TGAGAAGCTC CAGCAGCAGT TCAACTCACA CGTATTCAAG 
CTGGAGCAGG AGGAGTACAT GAAGGAGCAG ATCCCGTGGA CCCTGATTGA TTTCTATGAT 
AACCAGCCTT GTATCGACCT CATAGAAGCT AAGCTGGGCA TCTTGGACCT GTTGGATGAA 
GAATGTAAGG TCCCCAAAGG AACCGACCAG AACTGGACTC AGAAGCTCTA TGACAGGCAC 
TCTGGCAGCC AACACTTCCA GAAACCCCGC ATGTCCAACA AGGCCTTCAT TGTCATCCAC 
TTTGCAGATA AGGTGGAGTA CCTTTCAGAT GGTTTTCTGG AGAAAAACAG AGATACCGTG 
TACGAAGAAC AGATCAACAT CCTGAAGGCC AGCAAGTTCC CGCTGGTGGC TGACTTGTTC 
CATGATGGCA AAGACTCTGC TCCCACCGCC ACCGCTTCTT CCAAGATCAA CATTCGCTCC 
TCCAGACCCC CTGTGAAAGC CTCCAACAAG GAGCACAAGA AAACCGTGGG TTACCAGTTC 
CGCTCCTCCC TGCATCTGCT CATGGAGACA CTGAACGCCA CGACACCGCA CTATGTCCGC 
TGCATCAAGC CCAACGACGA GAAGCTGCCC TTCCATTTTA ACCCAAAGAG AGCAGTGCAG 
CAGCTCCGAG CCTGCGGCGT ATTGGAGACC ATCCGGATCA GCGCGGCTGG CTACCCATCC 
AGGTGGTCCT ACCATGACTT CTTCAACCGG TATCGGGTGC TGGTCAAGAA GAGAGACCTT 
GCCAACTCAG ACAAAAAGGC CATCTGCAGG TCTGTGCTGG AGAGCCTCAT CAAGGATCCT 
GACAAGTTCC AGTTTGGCCG CACCAAGATC TTCTTCCGGG CCGGCCAGGT GGCCTACCTG 
GAGAAGCTTC GGGCTGACAA GTTCCGGGCG GCCACCATCA TGATCCAGAA AGCCGTGCGG 
GGCTGGCTGC AGAAGGTGAA GTACCGCAGG TTGAAGGGAG CCACCTTAAT TCTGCAGAGG 
TACTGCCGAG GATACCTGGC CCGCAGACTG GCCAAGCATC TGCGGAGGAC CCGGGCGGCC 
GTGGTGCTCC AGAAGCAATA CCGCATGCAG AGGGCCCGCC GGGCCTACCA GAGGGTCCGC 
AGGGCCGCCG TCGTCATCCA GGCCTTTGCT CGGGGCATGT TTGTGCGGAG AATCTACCAC 
CAGGTCCTCA GGGAGCACAA GGCCACCATC ATCCAGAAGC ACGTGCGGGG CTGGATGGCT 
CGCAGGCGCT TCCAGCGGCT GCGGGGTGCG GCCGTTGTCA TCCAGTGCGG CTTCCGGAGG 
CTCAAGGCCA AGCAGGCGCT GAAGGCCCTC AGGATCGAGG CGCGCTCCGC GGAGCACCTG 
AAGCGCCTCA ACGTGGGCAT GGAGAACAAG ATTGTCCAGC TGCAGCGGAA GATCGATGAC 
CAGAACAAAG AGCTCAAGAC GCTGTCGGAG CAGCTGTCAG CAATCACCTC CACACACACC 
ATGGAAGTGG AGAAGCTGAA GAAGGAGGTG GCCTGCTACC AGCAGAGCCA GGGTGAGGAC 
AGAGGCCCGC AGTTGCAGGA GGAGGTGGAG AGCCTGCGGA CCGAGCTGCA GAGGGCCCAC 
TCAGAGCGCA AGGTCCTGGA GGACACCCAC ACCCGGGAGA AGGATGAGCT GAGGAAGCGA 
GTTGCAGACC TGGAACAAGA AAATGCACTC TTGAAAAATG AGAAGGAGCA GCTTAACAAC 
CAGATCCTGT GCCAGTCTAA AGATGAGTTT GCCCAGAACT CTGTCAAGGA AAACCTCATG 
ATGAAGAAGG AGCTGGAGGA GGAGCGCTCC CGTTATCAGA ACCTCGTGAA GGAGTATTCC 
CGGCTGGAGC AGAGATACGA CAACCTTCGG GATGAGATGA CCATCCTAAA GCAAACCCCA 
GGCCACCGGA GGAACCCATC AAACCAAAGC AGCTTAGAAT CTGACTCCAA TTACCCGTCC 
ATCTCCACGT CTGAGGTGGG AGACACAGAG GACACCCTCC AGCAGGTGGA GGAAATTGGC 
CTGGAGAAGG CAGCCATGGA CATGACCGTC TTCCTGAAGC TGCAGAAGAG GGTGCGGGAG 
CTGGAGCAGG AGAGGAAGAA GCTGCAGGTG CAGCTGGAGA AGAAAGAACA GCAGGACGGC 
AAGAGAGTCC CGGCGGACCA ACCAAGCAAT GACTCAGATT TGGACCAGGA TGCAGATCTA 
GCCTACAACA GTCTGAAGAG GCAGGAGCTG GAGTCGGAGA ACAAGAAGCT GAAGAATGAG 
CTGAACGAGC TGAGGAAAGC CGTGGCCGAC CAAGCCTCGG AGCACAGCCC GGCTGCCGCC 
GGCACCCCCG ACAGCTACAG CCTCCTGCTG AACCAGCTCC GGGTGGCCAA CGAGGAGCTG 
GAGGTGCGCA AGGAGGAAGT TCTCATCCTG CGGACCCAGA TCGTGAGCGC CGACCAGCGC 
CGCCTGGCGG GCAAGGGCTC GGAGCCAAAC ATCAATGCCC GAACCAGTTG GCCGAACAGC 
GAGAAGCACG TGGATCAGGA AGATGCCATC GAGGCCTATC ACGGGGTCTG CCAGACTAAC 
AGGTTGCTGG AGGCACAGCT GCAGGCCCAG AGCCGCGAGC ACGAGGAGGA GGTGGACAAT 
CTCAAGGCCC AGCTGGAGGC CCTGAAGGAG GAGATGGACA GACAGCAGCA GACCTTCTGC 
CAGACCCTGC TGCTCTCCCC AGAGGCCCAG GTGGAGTTCG GCATCCAGCA GGAGATGTCT 
CGGCTCACCA ACGAGAACCT GGACCTTAAA GAGTTAGTTG AGAAGCTGGA GAAGAACGAG 
AGGAAGCTCA AAAAGCAACT GAAGATTTAC ATGAAGAAGG TCCAGGACCT GGAAGCTGCC 
CAAGCCTTGG CCCAGAGCGA GAGGAAGCGG CACGAGCTCA ACAGGCAGGT CACCGTGCAG 
CGGAAGGAGA AGGACTTCCA AGGCATGCTG GAGTACCACA AGGAGGACGA GGCCCTGCTC 
ATTCGGAATC TGGTGACAGA GTTGAAACCG CAGGTGCTGG CGGGCGCGGT GCCCTGCCTC 
CCAGCCTACA TCCTCTACAT GTGCCTCCGG CACGCGGACT ACGTGAACGA CGACCTCAAG 
GTGCACTCCC TGCTGACCTC CACCATCAAC GGCATCAAGA AGGTCCTCAA GAAGCACAAT 
GAGGACTTTG AGATGACGTC GTTCTGGTTG TCCAACACCT GCCGCCTCCT GCACTGCTTG 
AAGCAGTACA GCGGGGACGA GGGCTTCATG ACTCAGAACA CTGCAAAGCA GAATGAGCAC 
TGTCTTAAGA ACTTTGACCT CACTGAATAC CGCCAGGTGC TGAGCGATCT TTCCATTCAG 
ATCTACCAGC AGCTCATTAA AATCGCCGAG GGCCTCTTGC AGCCTATGAT AGTGTCGGCC 
ATGTTGGAGA ACGAGAGCAT TCAGGGTCTG TCTGGGGTGA AGCCCACGGG CTACCGGAAG 
CGCACCTCCA GCATGCCGGA AGGAGACAAC TCGTACTGCC TGGAAGCCAT CATCCGCCAG 
ATGAACTCCT TCCACACGGT TATGTGCGAC CAGGGCCTGG ACCCCGAGAT CATCCTGCAG 
GTGTTCAGAC AGCTCTTCTA CATGATCAAC GCGGTGACGC TCAACAACCT GCTTCTGCGG 
AAGGACGTCT GCTCCTGGAG CACAGGCATG CAGCTCAGGT ACAACATTAG TCAGCTGGAG 
GAATGGCTTC GGGGAAGAAA CCTGCACCAG AGCGGAGCTG TTGAGACCAT GGAACCCCTG 
ATTCAGGCTG CCCAGCTCCT GCAGTTAAAG AAGAAAAGCC CCGAGGATGC TGAGGCCATC 
TGCTCCCTGT GCACCGCCCT CAGCACCCAG CAGATTGTCA AAATTTTGAA CCTTTATACC 
CCTCTGAATG AATTTGAAGA ACGGGTGACA GTGGCTTTTA TACGAACAAT CCAGGCACAA 
CTGCAAGATC GGAATGACCC TCAGCAGCTG CTATTAGACT TCAAGCACAT GTTTCCTGTT 
TTGTTCCCGT TTAATCCATC TTCTCTGACC ATGGACTCAA TCCACATCCC AGCATGTCTC 
AATCTGGAGT TCCTCAACGA AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840360.1
CDS	67..5553
Translation	MDPRGSSFPVLHCNTRQYTRVWIPDPDEVWRSAELTKDYKEGDKSLQLRLEDETIQEYPIDVQNNQLPFLRNPDILVGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYDQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFTTVSGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYRIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTSQGGNTVIEGVDDAEDFEKTRQAFTLVGVRESHQISIFKIIASILHLGNVEIQAERDGESCRVSPEDEHLSDFCRLLGVELSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARDALAKHIYAQLFHWIVEHVNKALHTSLKQHSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWTQKLYDRHSGSQHFQKPRMSNKAFIVIHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDGKDSAPTATASSKINIRSSRPPVKASNKEHKKTVGYQFRSSLHLLMETLNATTPHYVRCIKPNDEKLPFHFNPKRAVQQLRACGVLETIRISAAGYPSRWSYHDFFNRYRVLVKKRDLANSDKKAICRSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRAATIMIQKAVRGWLQKVKYRRLKGATLILQRYCRGYLARRLAKHLRRTRAAVVLQKQYRMQRARRAYQRVRRAAVVIQAFARGMFVRRIYHQVLREHKATIIQKHVRGWMARRRFQRLRGAAVVIQCGFRRLKAKQALKALRIEARSAEHLKRLNVGMENKIVQLQRKIDDQNKELKTLSEQLSAITSTHTMEVEKLKKEVACYQQSQGEDRGPQLQEEVESLRTELQRAHSERKVLEDTHTREKDELRKRVADLEQENALLKNEKEQLNNQILCQSKDEFAQNSVKENLMMKKELEEERSRYQNLVKEYSRLEQRYDNLRDEMTILKQTPGHRRNPSNQSSLESDSNYPSISTSEVGDTEDTLQQVEEIGLEKAAMDMTVFLKLQKRVRELEQERKKLQVQLEKKEQQDGKRVPADQPSNDSDLDQDADLAYNSLKRQELESENKKLKNELNELRKAVADQASEHSPAAAGTPDSYSLLLNQLRVANEELEVRKEEVLILRTQIVSADQRRLAGKGSEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNRLLEAQLQAQSREHEEEVDNLKAQLEALKEEMDRQQQTFCQTLLLSPEAQVEFGIQQEMSRLTNENLDLKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSERKRHELNRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTELKPQVLAGAVPCLPAYILYMCLRHADYVNDDLKVHSLLTSTINGIKKVLKKHNEDFEMTSFWLSNTCRLLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGLLQPMIVSAMLENESIQGLSGVKPTGYRKRTSSMPEGDNSYCLEAIIRQMNSFHTVMCDQGLDPEIILQVFRQLFYMINAVTLNNLLLRKDVCSWSTGMQLRYNISQLEEWLRGRNLHQSGAVETMEPLIQAAQLLQLKKKSPEDAEAICSLCTALSTQQIVKILNLYTPLNEFEERVTVAFIRTIQAQLQDRNDPQQLLLDFKHMFPVLFPFNPSSLTMDSIHIPACLNLEFLNEV

Target ORF information:

RefSeq Version	XM_024984592.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VB (MYO5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984592.1

ORF Insert Sequence:

ATGGACCCAA GAGGTAGTAG CTTCCCTGTG CTGCATTGTA ACACACGCCA GTACACAAGG 
GTCTGGATCC CTGACCCGGA TGAGGTGTGG CGCTCCGCTG AATTAACCAA GGACTACAAG 
GAGGGGGACA AGAGCCTCCA GCTCCGACTG GAAGATGAAA CCATTCAGGA GTACCCCATC 
GATGTCCAAA ACAACCAACT TCCCTTCCTG CGGAATCCAG ATATCCTGGT GGGAGAAAAT 
GACCTAACTG CCCTTAGCTA TCTCCATGAG CCTGCAGTTT TGCATAATTT AAAGGTCCGC 
TTCCTGGAGT CCAACCATAT CTACACTTAC TGTGGGATTG TGCTTGTTGC CATTAATCCT 
TATGATCAGT TGCCAATCTA CGGTCAAGAT GTCATCTATG CCTACAGTGG CCAGAACATG 
GGGGATATGG ACCCCCACAT CTTTGCTGTG GCAGAGGAAG CCTACAAGCA GATGGCCAGA 
GATGAGAAGA ACCAGTCCAT CATAGTCAGT GGGGAGTCTG GAGCTGGCAA GACGGTGTCG 
GCCAAGTATG CCATGCGCTA CTTCACCACG GTCAGTGGCT CAGCCAGTGA CACCAACATC 
GAAGAGAAAG TCCTGGCGTC CAGTCCCATC ATGGAGGCCA TCGGGAATGC TAAAACCACC 
CGCAACGACA ACAGCAGCCG TTTCGGGAAG TACATTCAGA TCGGCTTTGA CAAGAGGTAC 
CGCATCATCG GGGCCAACAT GAGGACCTAC CTGCTGGAGA AGTCCAGGGT GGTCTTTCAG 
GCAGACGACG AGAGGAATTA CCACATCTTT TACCAGCTCT GTGCGGCTGC CAGTCTTCCG 
GAATTTAAGG AGCTGGCACT AACCTGTGCT GAGGACTTCT TCTACACGTC ACAGGGAGGA 
AACACAGTCA TCGAGGGTGT GGATGATGCT GAGGACTTTG AGAAGACTCG ACAAGCCTTC 
ACGCTCGTTG GAGTGAGAGA GTCCCATCAG ATAAGCATTT TTAAGATAAT TGCTTCCATC 
CTGCACCTCG GAAATGTGGA GATTCAAGCC GAGCGCGATG GTGAATCCTG TAGAGTATCA 
CCTGAGGATG AACACCTAAG CGACTTCTGC CGCCTGCTGG GCGTGGAGCT CAGTCAGATG 
GAGCACTGGC TGTGCCATCG CAAGCTGGTC ACCACCTCGG AGACCTACGT CAAGACCATG 
TCCCTGCAGC AGGTGGTCAA CGCGCGCGAC GCCCTGGCCA AGCACATCTA TGCCCAGCTG 
TTCCACTGGA TCGTGGAGCA CGTCAACAAG GCCCTGCACA CCTCCCTCAA GCAGCACTCC 
TTCATTGGAG TCCTGGACAT CTACGGGTTT GAGACGTTTG AGGTGAACAG CTTCGAGCAG 
TTCTGTATCA ACTACGCCAA TGAGAAGCTC CAGCAGCAGT TCAACTCACA CGTATTCAAG 
CTGGAGCAGG AGGAGTACAT GAAGGAGCAG ATCCCGTGGA CCCTGATTGA TTTCTATGAT 
AACCAGCCTT GTATCGACCT CATAGAAGCT AAGCTGGGCA TCTTGGACCT GTTGGATGAA 
GAATGTAAGG TCCCCAAAGG AACCGACCAG AACTGGACTC AGAAGCTCTA TGACAGGCAC 
TCTGGCAGCC AACACTTCCA GAAACCCCGC ATGTCCAACA AGGCCTTCAT TGTCATCCAC 
TTTGCAGATA AGGTGGAGTA CCTTTCAGAT GGTTTTCTGG AGAAAAACAG AGATACCGTG 
TACGAAGAAC AGATCAACAT CCTGAAGGCC AGCAAGTTCC CGCTGGTGGC TGACTTGTTC 
CATGATGGCA AAGACTCTGC TCCCACCGCC ACCGCTTCTT CCAAGATCAA CATTCGCTCC 
TCCAGACCCC CTGTGAAAGC CTCCAACAAG GAGCACAAGA AAACCGTGGG TTACCAGTTC 
CGCTCCTCCC TGCATCTGCT CATGGAGACA CTGAACGCCA CGACACCGCA CTATGTCCGC 
TGCATCAAGC CCAACGACGA GAAGCTGCCC TTCCATTTTA ACCCAAAGAG AGCAGTGCAG 
CAGCTCCGAG CCTGCGGCGT ATTGGAGACC ATCCGGATCA GCGCGGCTGG CTACCCATCC 
AGGTGGTCCT ACCATGACTT CTTCAACCGG TATCGGGTGC TGGTCAAGAA GAGAGACCTT 
GCCAACTCAG ACAAAAAGGC CATCTGCAGG TCTGTGCTGG AGAGCCTCAT CAAGGATCCT 
GACAAGTTCC AGTTTGGCCG CACCAAGATC TTCTTCCGGG CCGGCCAGGT GGCCTACCTG 
GAGAAGCTTC GGGCTGACAA GTTCCGGGCG GCCACCATCA TGATCCAGAA AGCCGTGCGG 
GGCTGGCTGC AGAAGGTGAA GTACCGCAGG TTGAAGGGAG CCACCTTAAT TCTGCAGAGG 
TACTGCCGAG GATACCTGGC CCGCAGACTG GCCAAGCATC TGCGGAGGAC CCGGGCGGCC 
GTGGTGCTCC AGAAGCAATA CCGCATGCAG AGGGCCCGCC GGGCCTACCA GAGGGTCCGC 
AGGGCCGCCG TCGTCATCCA GGCCTTTGCT CGGGGCATGT TTGTGCGGAG AATCTACCAC 
CAGGTCCTCA GGGAGCACAA GGCCACCATC ATCCAGAAGC ACGTGCGGGG CTGGATGGCT 
CGCAGGCGCT TCCAGCGGCT GCGGGGTGCG GCCGTTGTCA TCCAGTGCGG CTTCCGGAGG 
CTCAAGGCCA AGCAGGCGCT GAAGGCCCTC AGGATCGAGG CGCGCTCCGC GGAGCACCTG 
AAGCGCCTCA ACGTGGGCAT GGAGAACAAG ATTGTCCAGC TGCAGCGGAA GATCGATGAC 
CAGAACAAAG AGCTCAAGAC GCTGTCGGAG CAGCTGTCAG CAATCACCTC CACACACACC 
ATGGAAGTGG AGAAGCTGAA GAAGGAGGTG GCCTGCTACC AGCAGAGCCA GGGTGAGGAC 
AGAGGCCCGC AGTTGCAGGA GGAGGTGGAG AGCCTGCGGA CCGAGCTGCA GAGGGCCCAC 
TCAGAGCGCA AGGTCCTGGA GGACACCCAC ACCCGGGAGA AGGATGAGCT GAGGAAGCGA 
GTTGCAGACC TGGAACAAGA AAATGCACTC TTGAAAAATG AGAAGGAGCA GCTTAACAAC 
CAGATCCTGT GCCAGTCTAA AGATGAGTTT GCCCAGAACT CTGTCAAGGA AAACCTCATG 
ATGAAGAAGG AGCTGGAGGA GGAGCGCTCC CGTTATCAGA ACCTCGTGAA GGAGTATTCC 
CGGCTGGAGC AGAGATACGA CAACCTTCGG GATGAGATGA CCATCCTAAA GCAAACCCCA 
GGCCACCGGA GGAACCCATC AAACCAAAGC AGCTTAGAAT CTGACTCCAA TTACCCGTCC 
ATCTCCACGT CTGAGGTGGG AGACACAGAG GACACCCTCC AGCAGGTGGA GGAAATTGGC 
CTGGAGAAGG CAGCCATGGA CATGACCGTC TTCCTGAAGC TGCAGAAGAG GGTGCGGGAG 
CTGGAGCAGG AGAGGAAGAA GCTGCAGGTG CAGCTGGAGA AGAAAGAACA GCAGGACGGC 
AAGAGAGTCC CGGCGGACCA ACCAAGCAAT GACTCAGATT TGGACCAGGA TGCAGATCTA 
GCCTACAACA GTCTGAAGAG GCAGGAGCTG GAGTCGGAGA ACAAGAAGCT GAAGAATGAG 
CTGAACGAGC TGAGGAAAGC CGTGGCCGAC CAAGCCTCGG AGCACAGCCC GGCTGCCGCC 
GGCACCCCCG ACAGCTACAG CCTCCTGCTG AACCAGCTCC GGGTGGCCAA CGAGGAGCTG 
GAGGTGCGCA AGGAGGAAGT TCTCATCCTG CGGACCCAGA TCGTGAGCGC CGACCAGCGC 
CGCCTGGCGG GCAAGGGCTC GGAGCCAAAC ATCAATGCCC GAACCAGTTG GCCGAACAGC 
GAGAAGCACG TGGATCAGGA AGATGCCATC GAGGCCTATC ACGGGGTCTG CCAGACTAAC 
AGGTTGCTGG AGGCACAGCT GCAGGCCCAG AGCCGCGAGC ACGAGGAGGA GGTGGACAAT 
CTCAAGGCCC AGCTGGAGGC CCTGAAGGAG GAGATGGACA GACAGCAGCA GACCTTCTGC 
CAGACCCTGC TGCTCTCCCC AGAGGCCCAG GTGGAGTTCG GCATCCAGCA GGAGATGTCT 
CGGCTCACCA ACGAGAACCT GGACCTTAAA GAGTTAGTTG AGAAGCTGGA GAAGAACGAG 
AGGAAGCTCA AAAAGCAACT GAAGATTTAC ATGAAGAAGG TCCAGGACCT GGAAGCTGCC 
CAAGCCTTGG CCCAGAGCGA GAGGAAGCGG CACGAGCTCA ACAGGCAGGT CACCGTGCAG 
CGGAAGGAGA AGGACTTCCA AGGCATGCTG GAGTACCACA AGGAGGACGA GGCCCTGCTC 
ATTCGGAATC TGGTGACAGA GTTGAAACCG CAGGTGCTGG CGGGCGCGGT GCCCTGCCTC 
CCAGCCTACA TCCTCTACAT GTGCCTCCGG CACGCGGACT ACGTGAACGA CGACCTCAAG 
GTGCACTCCC TGCTGACCTC CACCATCAAC GGCATCAAGA AGGTCCTCAA GAAGCACAAT 
GAGGACTTTG AGATGACGTC GTTCTGGTTG TCCAACACCT GCCGCCTCCT GCACTGCTTG 
AAGCAGTACA GCGGGGACGA GGGCTTCATG ACTCAGAACA CTGCAAAGCA GAATGAGCAC 
TGTCTTAAGA ACTTTGACCT CACTGAATAC CGCCAGGTGC TGAGCGATCT TTCCATTCAG 
ATCTACCAGC AGCTCATTAA AATCGCCGAG GGCCTCTTGC AGCCTATGAT AGTGTCGGCC 
ATGTTGGAGA ACGAGAGCAT TCAGGGTCTG TCTGGGGTGA AGCCCACGGG CTACCGGAAG 
CGCACCTCCA GCATGCCGGA AGGAGACAAC TCGTACTGCC TGGAAGCCAT CATCCGCCAG 
ATGAACTCCT TCCACACGGT TATGTGCGAC CAGGGCCTGG ACCCCGAGAT CATCCTGCAG 
GTGTTCAGAC AGCTCTTCTA CATGATCAAC GCGGTGACGC TCAACAACCT GCTTCTGCGG 
AAGGACGTCT GCTCCTGGAG CACAGGCATG CAGCTCAGGT ACAACATTAG TCAGCTGGAG 
GAATGGCTTC GGGGAAGAAA CCTGCACCAG AGCGGAGCTG TTGAGACCAT GGAACCCCTG 
ATTCAGGCTG CCCAGCTCCT GCAGTTAAAG AAGAAAAGCC CCGAGGATGC TGAGGCCATC 
TGCTCCCTGT GCACCGCCCT CAGCACCCAG CAGATTGTCA AAATTTTGAA CCTTTATACC 
CCTCTGAATG AATTTGAAGA ACGGGTGACA GTGGCTTTTA TACGAACAAT CCAGGCACAA 
CTGCAAGATC GGAATGACCC TCAGCAGCTG CTATTAGACT TCAAGCACAT GTTTCCTGTT 
TTGTTCCCGT TTAATCCATC TTCTCTGACC ATGGACTCAA TCCACATCCC AGCATGTCTC 
AATCTGGAGT TCCTCAACGA AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5B ( XM_015469177.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324663.1 Bos taurus myosin VB (MYO5B), transcript variant X2, mRNA.

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CloneID	OBa88362
Clone ID Related Accession (Same CDS sequence)	XM_015469177.1 , XM_015460147.1
Accession Version	XM_015469177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Vb isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTC TGGCTGAGAA CTGCTTTCAG AAGTCATCCC TCATTCAGTA CACAAGGGTC 
TGGATCCCTG ACCCGGATGA GGTGTGGCGC TCCGCTGAAT TAACCAAGGA CTACAAGGAG 
GGGGACAAGA GCCTCCAGCT CCGACTGGAA GATGAAACCA TTCAGGAGTA CCCCATCGAT 
GTCCAAAACA ACCAACTTCC CTTCCTGCGG AATCCAGATA TCCTGGTGGG AGAAAATGAC 
CTAACTGCCC TTAGCTATCT CCATGAGCCT GCAGTTTTGC ATAATTTAAA GGTCCGCTTC 
CTGGAGTCCA ACCATATCTA CACTTACTGT GGGATTGTGC TTGTTGCCAT TAATCCTTAT 
GATCAGTTGC CAATCTACGG TCAAGATGTC ATCTATGCCT ACAGTGGCCA GAACATGGGG 
GATATGGACC CCCACATCTT TGCTGTGGCA GAGGAAGCCT ACAAGCAGAT GGCCAGAGAT 
GAGAAGAACC AGTCCATCAT AGTCAGTGGG GAGTCTGGAG CTGGCAAGAC GGTGTCGGCC 
AAGTATGCCA TGCGCTACTT CACCACGGTC AGTGGCTCAG CCAGTGACAC CAACATCGAA 
GAGAAAGTCC TGGCGTCCAG TCCCATCATG GAGGCCATCG GGAATGCTAA AACCACCCGC 
AACGACAACA GCAGCCGTTT CGGGAAGTAC ATTCAGATCG GCTTTGACAA GAGGTACCGC 
ATCATCGGGG CCAACATGAG GACCTACCTG CTGGAGAAGT CCAGGGTGGT CTTTCAGGCA 
GACGACGAGA GGAATTACCA CATCTTTTAC CAGCTCTGTG CGGCTGCCAG TCTTCCGGAA 
TTTAAGGAGC TGGCACTAAC CTGTGCTGAG GACTTCTTCT ACACGTCACA GGGAGGAAAC 
ACAGTCATCG AGGGTGTGGA TGATGCTGAG GACTTTGAGA AGACTCGACA AGCCTTCACG 
CTCGTTGGAG TGAGAGAGTC CCATCAGATA AGCATTTTTA AGATAATTGC TTCCATCCTG 
CACCTCGGAA ATGTGGAGAT TCAAGCCGAG CGCGATGGTG AATCCTGTAG AGTATCACCT 
GAGGATGAAC ACCTAAGCGA CTTCTGCCGC CTGCTGGGCG TGGAGCTCAG TCAGATGGAG 
CACTGGCTGT GCCATCGCAA GCTGGTCACC ACCTCGGAGA CCTACGTCAA GACCATGTCC 
CTGCAGCAGG TGGTCAACGC GCGCGACGCC CTGGCCAAGC ACATCTATGC CCAGCTGTTC 
CACTGGATCG TGGAGCACGT CAACAAGGCC CTGCACACCT CCCTCAAGCA GCACTCCTTC 
ATTGGAGTCC TGGACATCTA CGGGTTTGAG ACGTTTGAGG TGAACAGCTT CGAGCAGTTC 
TGTATCAACT ACGCCAATGA GAAGCTCCAG CAGCAGTTCA ACTCACACGT ATTCAAGCTG 
GAGCAGGAGG AGTACATGAA GGAGCAGATC CCGTGGACCC TGATTGATTT CTATGATAAC 
CAGCCTTGTA TCGACCTCAT AGAAGCTAAG CTGGGCATCT TGGACCTGTT GGATGAAGAA 
TGTAAGGTCC CCAAAGGAAC CGACCAGAAC TGGACTCAGA AGCTCTATGA CAGGCACTCT 
GGCAGCCAAC ACTTCCAGAA ACCCCGCATG TCCAACAAGG CCTTCATTGT CATCCACTTT 
GCAGATAAGG TGGAGTACCT TTCAGATGGT TTTCTGGAGA AAAACAGAGA TACCGTGTAC 
GAAGAACAGA TCAACATCCT GAAGGCCAGC AAGTTCCCGC TGGTGGCTGA CTTGTTCCAT 
GATGGCAAAG ACTCTGCTCC CACCGCCACC GCTTCTTCCA AGATCAACAT TCGCTCCTCC 
AGACCCCCTG TGAAAGCCTC CAACAAGGAG CACAAGAAAA CCGTGGGTTA CCAGTTCCGC 
TCCTCCCTGC ATCTGCTCAT GGAGACACTG AACGCCACGA CACCGCACTA TGTCCGCTGC 
ATCAAGCCCA ACGACGAGAA GCTGCCCTTC CATTTTAACC CAAAGAGAGC AGTGCAGCAG 
CTCCGAGCCT GCGGCGTATT GGAGACCATC CGGATCAGCG CGGCTGGCTA CCCATCCAGG 
TGGTCCTACC ATGACTTCTT CAACCGGTAT CGGGTGCTGG TCAAGAAGAG AGACCTTGCC 
AACTCAGACA AAAAGGCCAT CTGCAGGTCT GTGCTGGAGA GCCTCATCAA GGATCCTGAC 
AAGTTCCAGT TTGGCCGCAC CAAGATCTTC TTCCGGGCCG GCCAGGTGGC CTACCTGGAG 
AAGCTTCGGG CTGACAAGTT CCGGGCGGCC ACCATCATGA TCCAGAAAGC CGTGCGGGGC 
TGGCTGCAGA AGGTGAAGTA CCGCAGGTTG AAGGGAGCCA CCTTAATTCT GCAGAGGTAC 
TGCCGAGGAT ACCTGGCCCG CAGACTGGCC AAGCATCTGC GGAGGACCCG GGCGGCCGTG 
GTGCTCCAGA AGCAATACCG CATGCAGAGG GCCCGCCGGG CCTACCAGAG GGTCCGCAGG 
GCCGCCGTCG TCATCCAGGC CTTTGCTCGG GGCATGTTTG TGCGGAGAAT CTACCACCAG 
GTCCTCAGGG AGCACAAGGC CACCATCATC CAGAAGCACG TGCGGGGCTG GATGGCTCGC 
AGGCGCTTCC AGCGGCTGCG GGGTGCGGCC GTTGTCATCC AGTGCGGCTT CCGGAGGCTC 
AAGGCCAAGC AGGCGCTGAA GGCCCTCAGG ATCGAGGCGC GCTCCGCGGA GCACCTGAAG 
CGCCTCAACG TGGGCATGGA GAACAAGATT GTCCAGCTGC AGCGGAAGAT CGATGACCAG 
AACAAAGAGC TCAAGACGCT GTCGGAGCAG CTGTCAGCAA TCACCTCCAC ACACACCATG 
GAAGTGGAGA AGCTGAAGAA GGAGGTGGCC TGCTACCAGC AGAGCCAGGG TGAGGACAGA 
GGCCCGCAGT TGCAGGAGGA GGTGGAGAGC CTGCGGACCG AGCTGCAGAG GGCCCACTCA 
GAGCGCAAGG TCCTGGAGGA CACCCACACC CGGGAGAAGG ATGAGCTGAG GAAGCGAGTT 
GCAGACCTGG AACAAGAAAA TGCACTCTTG AAAAATGAGA AGGAGCAGCT TAACAACCAG 
ATCCTGTGCC AGTCTAAAGA TGAGTTTGCC CAGAACTCTG TCAAGGAAAA CCTCATGATG 
AAGAAGGAGC TGGAGGAGGA GCGCTCCCGT TATCAGAACC TCGTGAAGGA GTATTCCCGG 
CTGGAGCAGA GATACGACAA CCTTCGGGAT GAGATGACCA TCCTAAAGCA AACCCCAGGC 
CACCGGAGGA ACCCATCAAA CCAAAGCAGC TTAGAATCTG ACTCCAATTA CCCGTCCATC 
TCCACGTCTG AGGTGGGAGA CACAGAGGAC ACCCTCCAGC AGGTGGAGGA AATTGGCCTG 
GAGAAGGCAG CCATGGACAT GACCGTCTTC CTGAAGCTGC AGAAGAGGGT GCGGGAGCTG 
GAGCAGGAGA GGAAGAAGCT GCAGGTGCAG CTGGAGAAGA AAGAACAGCA GGACGGCAAG 
AGAGTCCCGG CGGACCAACC AAGCAATGAC TCAGATTTGG ACCAGGATGC AGATCTAGCC 
TACAACAGTC TGAAGAGGCA GGAGCTGGAG TCGGAGAACA AGAAGCTGAA GAATGAGCTG 
AACGAGCTGA GGAAAGCCGT GGCCGACCAA GCCTCGGAGC ACAGCCCGGC TGCCGCCGGC 
ACCCCCGACA GCTACAGCCT CCTGCTGAAC CAGCTCCGGG TGGCCAACGA GGAGCTGGAG 
GTGCGCAAGG AGGAAGTTCT CATCCTGCGG ACCCAGATCG TGAGCGCCGA CCAGCGCCGC 
CTGGCGGGCA AGGGCTCGGA GCCAAACATC AATGCCCGAA CCAGTTGGCC GAACAGCGAG 
AAGCACGTGG ATCAGGAAGA TGCCATCGAG GCCTATCACG GGGTCTGCCA GACTAACAGG 
TTGCTGGAGG CACAGCTGCA GGCCCAGAGC CGCGAGCACG AGGAGGAGGT GGACAATCTC 
AAGGCCCAGC TGGAGGCCCT GAAGGAGGAG ATGGACAGAC AGCAGCAGAC CTTCTGCCAG 
ACCCTGCTGC TCTCCCCAGA GGCCCAGGTG GAGTTCGGCA TCCAGCAGGA GATGTCTCGG 
CTCACCAACG AGAACCTGGA CCTTAAAGAG TTAGTTGAGA AGCTGGAGAA GAACGAGAGG 
AAGCTCAAAA AGCAACTGAA GATTTACATG AAGAAGGTCC AGGACCTGGA AGCTGCCCAA 
GCCTTGGCCC AGAGCGAGAG GAAGCGGCAC GAGCTCAACA GGCAGGTCAC CGTGCAGCGG 
AAGGAGAAGG ACTTCCAAGG CATGCTGGAG TACCACAAGG AGGACGAGGC CCTGCTCATT 
CGGAATCTGG TGACAGAGTT GAAACCGCAG GTGCTGGCGG GCGCGGTGCC CTGCCTCCCA 
GCCTACATCC TCTACATGTG CCTCCGGCAC GCGGACTACG TGAACGACGA CCTCAAGGTG 
CACTCCCTGC TGACCTCCAC CATCAACGGC ATCAAGAAGG TCCTCAAGAA GCACAATGAG 
GACTTTGAGA TGACGTCGTT CTGGTTGTCC AACACCTGCC GCCTCCTGCA CTGCTTGAAG 
CAGTACAGCG GGGACGAGGG CTTCATGACT CAGAACACTG CAAAGCAGAA TGAGCACTGT 
CTTAAGAACT TTGACCTCAC TGAATACCGC CAGGTGCTGA GCGATCTTTC CATTCAGATC 
TACCAGCAGC TCATTAAAAT CGCCGAGGGC CTCTTGCAGC CTATGATAGT GTCGGCCATG 
TTGGAGAACG AGAGCATTCA GGGTCTGTCT GGGGTGAAGC CCACGGGCTA CCGGAAGCGC 
ACCTCCAGCA TGCCGGAAGG AGACAACTCG TACTGCCTGG AAGCCATCAT CCGCCAGATG 
AACTCCTTCC ACACGGTTAT GTGCGACCAG GGCCTGGACC CCGAGATCAT CCTGCAGGTG 
TTCAGACAGC TCTTCTACAT GATCAACGCG GTGACGCTCA ACAACCTGCT TCTGCGGAAG 
GACGTCTGCT CCTGGAGCAC AGGCATGCAG CTCAGGTACA ACATTAGTCA GCTGGAGGAA 
TGGCTTCGGG GAAGAAACCT GCACCAGAGC GGAGCTGTTG AGACCATGGA ACCCCTGATT 
CAGGCTGCCC AGCTCCTGCA GTTAAAGAAG AAAAGCCCCG AGGATGCTGA GGCCATCTGC 
TCCCTGTGCA CCGCCCTCAG CACCCAGCAG ATTGTCAAAA TTTTGAACCT TTATACCCCT 
CTGAATGAAT TTGAAGAACG GGTGACAGTG GCTTTTATAC GAACAATCCA GGCACAACTG 
CAAGATCGGA ATGACCCTCA GCAGCTGCTA TTAGACTTCA AGCACATGTT TCCTGTTTTG 
TTCCCGTTTA ATCCATCTTC TCTGACCATG GACTCAATCC ACATCCCAGC ATGTCTCAAT 
CTGGAGTTCC TCAACGAAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324663.1
CDS	38..5521
Translation	MDALAENCFQKSSLIQYTRVWIPDPDEVWRSAELTKDYKEGDKSLQLRLEDETIQEYPIDVQNNQLPFLRNPDILVGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYDQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFTTVSGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYRIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTSQGGNTVIEGVDDAEDFEKTRQAFTLVGVRESHQISIFKIIASILHLGNVEIQAERDGESCRVSPEDEHLSDFCRLLGVELSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARDALAKHIYAQLFHWIVEHVNKALHTSLKQHSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWTQKLYDRHSGSQHFQKPRMSNKAFIVIHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDGKDSAPTATASSKINIRSSRPPVKASNKEHKKTVGYQFRSSLHLLMETLNATTPHYVRCIKPNDEKLPFHFNPKRAVQQLRACGVLETIRISAAGYPSRWSYHDFFNRYRVLVKKRDLANSDKKAICRSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRAATIMIQKAVRGWLQKVKYRRLKGATLILQRYCRGYLARRLAKHLRRTRAAVVLQKQYRMQRARRAYQRVRRAAVVIQAFARGMFVRRIYHQVLREHKATIIQKHVRGWMARRRFQRLRGAAVVIQCGFRRLKAKQALKALRIEARSAEHLKRLNVGMENKIVQLQRKIDDQNKELKTLSEQLSAITSTHTMEVEKLKKEVACYQQSQGEDRGPQLQEEVESLRTELQRAHSERKVLEDTHTREKDELRKRVADLEQENALLKNEKEQLNNQILCQSKDEFAQNSVKENLMMKKELEEERSRYQNLVKEYSRLEQRYDNLRDEMTILKQTPGHRRNPSNQSSLESDSNYPSISTSEVGDTEDTLQQVEEIGLEKAAMDMTVFLKLQKRVRELEQERKKLQVQLEKKEQQDGKRVPADQPSNDSDLDQDADLAYNSLKRQELESENKKLKNELNELRKAVADQASEHSPAAAGTPDSYSLLLNQLRVANEELEVRKEEVLILRTQIVSADQRRLAGKGSEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNRLLEAQLQAQSREHEEEVDNLKAQLEALKEEMDRQQQTFCQTLLLSPEAQVEFGIQQEMSRLTNENLDLKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSERKRHELNRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTELKPQVLAGAVPCLPAYILYMCLRHADYVNDDLKVHSLLTSTINGIKKVLKKHNEDFEMTSFWLSNTCRLLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGLLQPMIVSAMLENESIQGLSGVKPTGYRKRTSSMPEGDNSYCLEAIIRQMNSFHTVMCDQGLDPEIILQVFRQLFYMINAVTLNNLLLRKDVCSWSTGMQLRYNISQLEEWLRGRNLHQSGAVETMEPLIQAAQLLQLKKKSPEDAEAICSLCTALSTQQIVKILNLYTPLNEFEERVTVAFIRTIQAQLQDRNDPQQLLLDFKHMFPVLFPFNPSSLTMDSIHIPACLNLEFLNEV

Target ORF information:

RefSeq Version	XM_015469177.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VB (MYO5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469177.1

ORF Insert Sequence:

ATGGATGCTC TGGCTGAGAA CTGCTTTCAG AAGTCATCCC TCATTCAGTA CACAAGGGTC 
TGGATCCCTG ACCCGGATGA GGTGTGGCGC TCCGCTGAAT TAACCAAGGA CTACAAGGAG 
GGGGACAAGA GCCTCCAGCT CCGACTGGAA GATGAAACCA TTCAGGAGTA CCCCATCGAT 
GTCCAAAACA ACCAACTTCC CTTCCTGCGG AATCCAGATA TCCTGGTGGG AGAAAATGAC 
CTAACTGCCC TTAGCTATCT CCATGAGCCT GCAGTTTTGC ATAATTTAAA GGTCCGCTTC 
CTGGAGTCCA ACCATATCTA CACTTACTGT GGGATTGTGC TTGTTGCCAT TAATCCTTAT 
GATCAGTTGC CAATCTACGG TCAAGATGTC ATCTATGCCT ACAGTGGCCA GAACATGGGG 
GATATGGACC CCCACATCTT TGCTGTGGCA GAGGAAGCCT ACAAGCAGAT GGCCAGAGAT 
GAGAAGAACC AGTCCATCAT AGTCAGTGGG GAGTCTGGAG CTGGCAAGAC GGTGTCGGCC 
AAGTATGCCA TGCGCTACTT CACCACGGTC AGTGGCTCAG CCAGTGACAC CAACATCGAA 
GAGAAAGTCC TGGCGTCCAG TCCCATCATG GAGGCCATCG GGAATGCTAA AACCACCCGC 
AACGACAACA GCAGCCGTTT CGGGAAGTAC ATTCAGATCG GCTTTGACAA GAGGTACCGC 
ATCATCGGGG CCAACATGAG GACCTACCTG CTGGAGAAGT CCAGGGTGGT CTTTCAGGCA 
GACGACGAGA GGAATTACCA CATCTTTTAC CAGCTCTGTG CGGCTGCCAG TCTTCCGGAA 
TTTAAGGAGC TGGCACTAAC CTGTGCTGAG GACTTCTTCT ACACGTCACA GGGAGGAAAC 
ACAGTCATCG AGGGTGTGGA TGATGCTGAG GACTTTGAGA AGACTCGACA AGCCTTCACG 
CTCGTTGGAG TGAGAGAGTC CCATCAGATA AGCATTTTTA AGATAATTGC TTCCATCCTG 
CACCTCGGAA ATGTGGAGAT TCAAGCCGAG CGCGATGGTG AATCCTGTAG AGTATCACCT 
GAGGATGAAC ACCTAAGCGA CTTCTGCCGC CTGCTGGGCG TGGAGCTCAG TCAGATGGAG 
CACTGGCTGT GCCATCGCAA GCTGGTCACC ACCTCGGAGA CCTACGTCAA GACCATGTCC 
CTGCAGCAGG TGGTCAACGC GCGCGACGCC CTGGCCAAGC ACATCTATGC CCAGCTGTTC 
CACTGGATCG TGGAGCACGT CAACAAGGCC CTGCACACCT CCCTCAAGCA GCACTCCTTC 
ATTGGAGTCC TGGACATCTA CGGGTTTGAG ACGTTTGAGG TGAACAGCTT CGAGCAGTTC 
TGTATCAACT ACGCCAATGA GAAGCTCCAG CAGCAGTTCA ACTCACACGT ATTCAAGCTG 
GAGCAGGAGG AGTACATGAA GGAGCAGATC CCGTGGACCC TGATTGATTT CTATGATAAC 
CAGCCTTGTA TCGACCTCAT AGAAGCTAAG CTGGGCATCT TGGACCTGTT GGATGAAGAA 
TGTAAGGTCC CCAAAGGAAC CGACCAGAAC TGGACTCAGA AGCTCTATGA CAGGCACTCT 
GGCAGCCAAC ACTTCCAGAA ACCCCGCATG TCCAACAAGG CCTTCATTGT CATCCACTTT 
GCAGATAAGG TGGAGTACCT TTCAGATGGT TTTCTGGAGA AAAACAGAGA TACCGTGTAC 
GAAGAACAGA TCAACATCCT GAAGGCCAGC AAGTTCCCGC TGGTGGCTGA CTTGTTCCAT 
GATGGCAAAG ACTCTGCTCC CACCGCCACC GCTTCTTCCA AGATCAACAT TCGCTCCTCC 
AGACCCCCTG TGAAAGCCTC CAACAAGGAG CACAAGAAAA CCGTGGGTTA CCAGTTCCGC 
TCCTCCCTGC ATCTGCTCAT GGAGACACTG AACGCCACGA CACCGCACTA TGTCCGCTGC 
ATCAAGCCCA ACGACGAGAA GCTGCCCTTC CATTTTAACC CAAAGAGAGC AGTGCAGCAG 
CTCCGAGCCT GCGGCGTATT GGAGACCATC CGGATCAGCG CGGCTGGCTA CCCATCCAGG 
TGGTCCTACC ATGACTTCTT CAACCGGTAT CGGGTGCTGG TCAAGAAGAG AGACCTTGCC 
AACTCAGACA AAAAGGCCAT CTGCAGGTCT GTGCTGGAGA GCCTCATCAA GGATCCTGAC 
AAGTTCCAGT TTGGCCGCAC CAAGATCTTC TTCCGGGCCG GCCAGGTGGC CTACCTGGAG 
AAGCTTCGGG CTGACAAGTT CCGGGCGGCC ACCATCATGA TCCAGAAAGC CGTGCGGGGC 
TGGCTGCAGA AGGTGAAGTA CCGCAGGTTG AAGGGAGCCA CCTTAATTCT GCAGAGGTAC 
TGCCGAGGAT ACCTGGCCCG CAGACTGGCC AAGCATCTGC GGAGGACCCG GGCGGCCGTG 
GTGCTCCAGA AGCAATACCG CATGCAGAGG GCCCGCCGGG CCTACCAGAG GGTCCGCAGG 
GCCGCCGTCG TCATCCAGGC CTTTGCTCGG GGCATGTTTG TGCGGAGAAT CTACCACCAG 
GTCCTCAGGG AGCACAAGGC CACCATCATC CAGAAGCACG TGCGGGGCTG GATGGCTCGC 
AGGCGCTTCC AGCGGCTGCG GGGTGCGGCC GTTGTCATCC AGTGCGGCTT CCGGAGGCTC 
AAGGCCAAGC AGGCGCTGAA GGCCCTCAGG ATCGAGGCGC GCTCCGCGGA GCACCTGAAG 
CGCCTCAACG TGGGCATGGA GAACAAGATT GTCCAGCTGC AGCGGAAGAT CGATGACCAG 
AACAAAGAGC TCAAGACGCT GTCGGAGCAG CTGTCAGCAA TCACCTCCAC ACACACCATG 
GAAGTGGAGA AGCTGAAGAA GGAGGTGGCC TGCTACCAGC AGAGCCAGGG TGAGGACAGA 
GGCCCGCAGT TGCAGGAGGA GGTGGAGAGC CTGCGGACCG AGCTGCAGAG GGCCCACTCA 
GAGCGCAAGG TCCTGGAGGA CACCCACACC CGGGAGAAGG ATGAGCTGAG GAAGCGAGTT 
GCAGACCTGG AACAAGAAAA TGCACTCTTG AAAAATGAGA AGGAGCAGCT TAACAACCAG 
ATCCTGTGCC AGTCTAAAGA TGAGTTTGCC CAGAACTCTG TCAAGGAAAA CCTCATGATG 
AAGAAGGAGC TGGAGGAGGA GCGCTCCCGT TATCAGAACC TCGTGAAGGA GTATTCCCGG 
CTGGAGCAGA GATACGACAA CCTTCGGGAT GAGATGACCA TCCTAAAGCA AACCCCAGGC 
CACCGGAGGA ACCCATCAAA CCAAAGCAGC TTAGAATCTG ACTCCAATTA CCCGTCCATC 
TCCACGTCTG AGGTGGGAGA CACAGAGGAC ACCCTCCAGC AGGTGGAGGA AATTGGCCTG 
GAGAAGGCAG CCATGGACAT GACCGTCTTC CTGAAGCTGC AGAAGAGGGT GCGGGAGCTG 
GAGCAGGAGA GGAAGAAGCT GCAGGTGCAG CTGGAGAAGA AAGAACAGCA GGACGGCAAG 
AGAGTCCCGG CGGACCAACC AAGCAATGAC TCAGATTTGG ACCAGGATGC AGATCTAGCC 
TACAACAGTC TGAAGAGGCA GGAGCTGGAG TCGGAGAACA AGAAGCTGAA GAATGAGCTG 
AACGAGCTGA GGAAAGCCGT GGCCGACCAA GCCTCGGAGC ACAGCCCGGC TGCCGCCGGC 
ACCCCCGACA GCTACAGCCT CCTGCTGAAC CAGCTCCGGG TGGCCAACGA GGAGCTGGAG 
GTGCGCAAGG AGGAAGTTCT CATCCTGCGG ACCCAGATCG TGAGCGCCGA CCAGCGCCGC 
CTGGCGGGCA AGGGCTCGGA GCCAAACATC AATGCCCGAA CCAGTTGGCC GAACAGCGAG 
AAGCACGTGG ATCAGGAAGA TGCCATCGAG GCCTATCACG GGGTCTGCCA GACTAACAGG 
TTGCTGGAGG CACAGCTGCA GGCCCAGAGC CGCGAGCACG AGGAGGAGGT GGACAATCTC 
AAGGCCCAGC TGGAGGCCCT GAAGGAGGAG ATGGACAGAC AGCAGCAGAC CTTCTGCCAG 
ACCCTGCTGC TCTCCCCAGA GGCCCAGGTG GAGTTCGGCA TCCAGCAGGA GATGTCTCGG 
CTCACCAACG AGAACCTGGA CCTTAAAGAG TTAGTTGAGA AGCTGGAGAA GAACGAGAGG 
AAGCTCAAAA AGCAACTGAA GATTTACATG AAGAAGGTCC AGGACCTGGA AGCTGCCCAA 
GCCTTGGCCC AGAGCGAGAG GAAGCGGCAC GAGCTCAACA GGCAGGTCAC CGTGCAGCGG 
AAGGAGAAGG ACTTCCAAGG CATGCTGGAG TACCACAAGG AGGACGAGGC CCTGCTCATT 
CGGAATCTGG TGACAGAGTT GAAACCGCAG GTGCTGGCGG GCGCGGTGCC CTGCCTCCCA 
GCCTACATCC TCTACATGTG CCTCCGGCAC GCGGACTACG TGAACGACGA CCTCAAGGTG 
CACTCCCTGC TGACCTCCAC CATCAACGGC ATCAAGAAGG TCCTCAAGAA GCACAATGAG 
GACTTTGAGA TGACGTCGTT CTGGTTGTCC AACACCTGCC GCCTCCTGCA CTGCTTGAAG 
CAGTACAGCG GGGACGAGGG CTTCATGACT CAGAACACTG CAAAGCAGAA TGAGCACTGT 
CTTAAGAACT TTGACCTCAC TGAATACCGC CAGGTGCTGA GCGATCTTTC CATTCAGATC 
TACCAGCAGC TCATTAAAAT CGCCGAGGGC CTCTTGCAGC CTATGATAGT GTCGGCCATG 
TTGGAGAACG AGAGCATTCA GGGTCTGTCT GGGGTGAAGC CCACGGGCTA CCGGAAGCGC 
ACCTCCAGCA TGCCGGAAGG AGACAACTCG TACTGCCTGG AAGCCATCAT CCGCCAGATG 
AACTCCTTCC ACACGGTTAT GTGCGACCAG GGCCTGGACC CCGAGATCAT CCTGCAGGTG 
TTCAGACAGC TCTTCTACAT GATCAACGCG GTGACGCTCA ACAACCTGCT TCTGCGGAAG 
GACGTCTGCT CCTGGAGCAC AGGCATGCAG CTCAGGTACA ACATTAGTCA GCTGGAGGAA 
TGGCTTCGGG GAAGAAACCT GCACCAGAGC GGAGCTGTTG AGACCATGGA ACCCCTGATT 
CAGGCTGCCC AGCTCCTGCA GTTAAAGAAG AAAAGCCCCG AGGATGCTGA GGCCATCTGC 
TCCCTGTGCA CCGCCCTCAG CACCCAGCAG ATTGTCAAAA TTTTGAACCT TTATACCCCT 
CTGAATGAAT TTGAAGAACG GGTGACAGTG GCTTTTATAC GAACAATCCA GGCACAACTG 
CAAGATCGGA ATGACCCTCA GCAGCTGCTA TTAGACTTCA AGCACATGTT TCCTGTTTTG 
TTCCCGTTTA ATCCATCTTC TCTGACCATG GACTCAATCC ACATCCCAGC ATGTCTCAAT 
CTGGAGTTCC TCAACGAAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5B ( XM_015460147.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315633.1 Bos taurus myosin VB (MYO5B), transcript variant X2, mRNA.

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CloneID	OBa88362
Clone ID Related Accession (Same CDS sequence)	XM_015469177.1 , XM_015460147.1
Accession Version	XM_015460147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Vb isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTC TGGCTGAGAA CTGCTTTCAG AAGTCATCCC TCATTCAGTA CACAAGGGTC 
TGGATCCCTG ACCCGGATGA GGTGTGGCGC TCCGCTGAAT TAACCAAGGA CTACAAGGAG 
GGGGACAAGA GCCTCCAGCT CCGACTGGAA GATGAAACCA TTCAGGAGTA CCCCATCGAT 
GTCCAAAACA ACCAACTTCC CTTCCTGCGG AATCCAGATA TCCTGGTGGG AGAAAATGAC 
CTAACTGCCC TTAGCTATCT CCATGAGCCT GCAGTTTTGC ATAATTTAAA GGTCCGCTTC 
CTGGAGTCCA ACCATATCTA CACTTACTGT GGGATTGTGC TTGTTGCCAT TAATCCTTAT 
GATCAGTTGC CAATCTACGG TCAAGATGTC ATCTATGCCT ACAGTGGCCA GAACATGGGG 
GATATGGACC CCCACATCTT TGCTGTGGCA GAGGAAGCCT ACAAGCAGAT GGCCAGAGAT 
GAGAAGAACC AGTCCATCAT AGTCAGTGGG GAGTCTGGAG CTGGCAAGAC GGTGTCGGCC 
AAGTATGCCA TGCGCTACTT CACCACGGTC AGTGGCTCAG CCAGTGACAC CAACATCGAA 
GAGAAAGTCC TGGCGTCCAG TCCCATCATG GAGGCCATCG GGAATGCTAA AACCACCCGC 
AACGACAACA GCAGCCGTTT CGGGAAGTAC ATTCAGATCG GCTTTGACAA GAGGTACCGC 
ATCATCGGGG CCAACATGAG GACCTACCTG CTGGAGAAGT CCAGGGTGGT CTTTCAGGCA 
GACGACGAGA GGAATTACCA CATCTTTTAC CAGCTCTGTG CGGCTGCCAG TCTTCCGGAA 
TTTAAGGAGC TGGCACTAAC CTGTGCTGAG GACTTCTTCT ACACGTCACA GGGAGGAAAC 
ACAGTCATCG AGGGTGTGGA TGATGCTGAG GACTTTGAGA AGACTCGACA AGCCTTCACG 
CTCGTTGGAG TGAGAGAGTC CCATCAGATA AGCATTTTTA AGATAATTGC TTCCATCCTG 
CACCTCGGAA ATGTGGAGAT TCAAGCCGAG CGCGATGGTG AATCCTGTAG AGTATCACCT 
GAGGATGAAC ACCTAAGCGA CTTCTGCCGC CTGCTGGGCG TGGAGCTCAG TCAGATGGAG 
CACTGGCTGT GCCATCGCAA GCTGGTCACC ACCTCGGAGA CCTACGTCAA GACCATGTCC 
CTGCAGCAGG TGGTCAACGC GCGCGACGCC CTGGCCAAGC ACATCTATGC CCAGCTGTTC 
CACTGGATCG TGGAGCACGT CAACAAGGCC CTGCACACCT CCCTCAAGCA GCACTCCTTC 
ATTGGAGTCC TGGACATCTA CGGGTTTGAG ACGTTTGAGG TGAACAGCTT CGAGCAGTTC 
TGTATCAACT ACGCCAATGA GAAGCTCCAG CAGCAGTTCA ACTCACACGT ATTCAAGCTG 
GAGCAGGAGG AGTACATGAA GGAGCAGATC CCGTGGACCC TGATTGATTT CTATGATAAC 
CAGCCTTGTA TCGACCTCAT AGAAGCTAAG CTGGGCATCT TGGACCTGTT GGATGAAGAA 
TGTAAGGTCC CCAAAGGAAC CGACCAGAAC TGGACTCAGA AGCTCTATGA CAGGCACTCT 
GGCAGCCAAC ACTTCCAGAA ACCCCGCATG TCCAACAAGG CCTTCATTGT CATCCACTTT 
GCAGATAAGG TGGAGTACCT TTCAGATGGT TTTCTGGAGA AAAACAGAGA TACCGTGTAC 
GAAGAACAGA TCAACATCCT GAAGGCCAGC AAGTTCCCGC TGGTGGCTGA CTTGTTCCAT 
GATGGCAAAG ACTCTGCTCC CACCGCCACC GCTTCTTCCA AGATCAACAT TCGCTCCTCC 
AGACCCCCTG TGAAAGCCTC CAACAAGGAG CACAAGAAAA CCGTGGGTTA CCAGTTCCGC 
TCCTCCCTGC ATCTGCTCAT GGAGACACTG AACGCCACGA CACCGCACTA TGTCCGCTGC 
ATCAAGCCCA ACGACGAGAA GCTGCCCTTC CATTTTAACC CAAAGAGAGC AGTGCAGCAG 
CTCCGAGCCT GCGGCGTATT GGAGACCATC CGGATCAGCG CGGCTGGCTA CCCATCCAGG 
TGGTCCTACC ATGACTTCTT CAACCGGTAT CGGGTGCTGG TCAAGAAGAG AGACCTTGCC 
AACTCAGACA AAAAGGCCAT CTGCAGGTCT GTGCTGGAGA GCCTCATCAA GGATCCTGAC 
AAGTTCCAGT TTGGCCGCAC CAAGATCTTC TTCCGGGCCG GCCAGGTGGC CTACCTGGAG 
AAGCTTCGGG CTGACAAGTT CCGGGCGGCC ACCATCATGA TCCAGAAAGC CGTGCGGGGC 
TGGCTGCAGA AGGTGAAGTA CCGCAGGTTG AAGGGAGCCA CCTTAATTCT GCAGAGGTAC 
TGCCGAGGAT ACCTGGCCCG CAGACTGGCC AAGCATCTGC GGAGGACCCG GGCGGCCGTG 
GTGCTCCAGA AGCAATACCG CATGCAGAGG GCCCGCCGGG CCTACCAGAG GGTCCGCAGG 
GCCGCCGTCG TCATCCAGGC CTTTGCTCGG GGCATGTTTG TGCGGAGAAT CTACCACCAG 
GTCCTCAGGG AGCACAAGGC CACCATCATC CAGAAGCACG TGCGGGGCTG GATGGCTCGC 
AGGCGCTTCC AGCGGCTGCG GGGTGCGGCC GTTGTCATCC AGTGCGGCTT CCGGAGGCTC 
AAGGCCAAGC AGGCGCTGAA GGCCCTCAGG ATCGAGGCGC GCTCCGCGGA GCACCTGAAG 
CGCCTCAACG TGGGCATGGA GAACAAGATT GTCCAGCTGC AGCGGAAGAT CGATGACCAG 
AACAAAGAGC TCAAGACGCT GTCGGAGCAG CTGTCAGCAA TCACCTCCAC ACACACCATG 
GAAGTGGAGA AGCTGAAGAA GGAGGTGGCC TGCTACCAGC AGAGCCAGGG TGAGGACAGA 
GGCCCGCAGT TGCAGGAGGA GGTGGAGAGC CTGCGGACCG AGCTGCAGAG GGCCCACTCA 
GAGCGCAAGG TCCTGGAGGA CACCCACACC CGGGAGAAGG ATGAGCTGAG GAAGCGAGTT 
GCAGACCTGG AACAAGAAAA TGCACTCTTG AAAAATGAGA AGGAGCAGCT TAACAACCAG 
ATCCTGTGCC AGTCTAAAGA TGAGTTTGCC CAGAACTCTG TCAAGGAAAA CCTCATGATG 
AAGAAGGAGC TGGAGGAGGA GCGCTCCCGT TATCAGAACC TCGTGAAGGA GTATTCCCGG 
CTGGAGCAGA GATACGACAA CCTTCGGGAT GAGATGACCA TCCTAAAGCA AACCCCAGGC 
CACCGGAGGA ACCCATCAAA CCAAAGCAGC TTAGAATCTG ACTCCAATTA CCCGTCCATC 
TCCACGTCTG AGGTGGGAGA CACAGAGGAC ACCCTCCAGC AGGTGGAGGA AATTGGCCTG 
GAGAAGGCAG CCATGGACAT GACCGTCTTC CTGAAGCTGC AGAAGAGGGT GCGGGAGCTG 
GAGCAGGAGA GGAAGAAGCT GCAGGTGCAG CTGGAGAAGA AAGAACAGCA GGACGGCAAG 
AGAGTCCCGG CGGACCAACC AAGCAATGAC TCAGATTTGG ACCAGGATGC AGATCTAGCC 
TACAACAGTC TGAAGAGGCA GGAGCTGGAG TCGGAGAACA AGAAGCTGAA GAATGAGCTG 
AACGAGCTGA GGAAAGCCGT GGCCGACCAA GCCTCGGAGC ACAGCCCGGC TGCCGCCGGC 
ACCCCCGACA GCTACAGCCT CCTGCTGAAC CAGCTCCGGG TGGCCAACGA GGAGCTGGAG 
GTGCGCAAGG AGGAAGTTCT CATCCTGCGG ACCCAGATCG TGAGCGCCGA CCAGCGCCGC 
CTGGCGGGCA AGGGCTCGGA GCCAAACATC AATGCCCGAA CCAGTTGGCC GAACAGCGAG 
AAGCACGTGG ATCAGGAAGA TGCCATCGAG GCCTATCACG GGGTCTGCCA GACTAACAGG 
TTGCTGGAGG CACAGCTGCA GGCCCAGAGC CGCGAGCACG AGGAGGAGGT GGACAATCTC 
AAGGCCCAGC TGGAGGCCCT GAAGGAGGAG ATGGACAGAC AGCAGCAGAC CTTCTGCCAG 
ACCCTGCTGC TCTCCCCAGA GGCCCAGGTG GAGTTCGGCA TCCAGCAGGA GATGTCTCGG 
CTCACCAACG AGAACCTGGA CCTTAAAGAG TTAGTTGAGA AGCTGGAGAA GAACGAGAGG 
AAGCTCAAAA AGCAACTGAA GATTTACATG AAGAAGGTCC AGGACCTGGA AGCTGCCCAA 
GCCTTGGCCC AGAGCGAGAG GAAGCGGCAC GAGCTCAACA GGCAGGTCAC CGTGCAGCGG 
AAGGAGAAGG ACTTCCAAGG CATGCTGGAG TACCACAAGG AGGACGAGGC CCTGCTCATT 
CGGAATCTGG TGACAGAGTT GAAACCGCAG GTGCTGGCGG GCGCGGTGCC CTGCCTCCCA 
GCCTACATCC TCTACATGTG CCTCCGGCAC GCGGACTACG TGAACGACGA CCTCAAGGTG 
CACTCCCTGC TGACCTCCAC CATCAACGGC ATCAAGAAGG TCCTCAAGAA GCACAATGAG 
GACTTTGAGA TGACGTCGTT CTGGTTGTCC AACACCTGCC GCCTCCTGCA CTGCTTGAAG 
CAGTACAGCG GGGACGAGGG CTTCATGACT CAGAACACTG CAAAGCAGAA TGAGCACTGT 
CTTAAGAACT TTGACCTCAC TGAATACCGC CAGGTGCTGA GCGATCTTTC CATTCAGATC 
TACCAGCAGC TCATTAAAAT CGCCGAGGGC CTCTTGCAGC CTATGATAGT GTCGGCCATG 
TTGGAGAACG AGAGCATTCA GGGTCTGTCT GGGGTGAAGC CCACGGGCTA CCGGAAGCGC 
ACCTCCAGCA TGCCGGAAGG AGACAACTCG TACTGCCTGG AAGCCATCAT CCGCCAGATG 
AACTCCTTCC ACACGGTTAT GTGCGACCAG GGCCTGGACC CCGAGATCAT CCTGCAGGTG 
TTCAGACAGC TCTTCTACAT GATCAACGCG GTGACGCTCA ACAACCTGCT TCTGCGGAAG 
GACGTCTGCT CCTGGAGCAC AGGCATGCAG CTCAGGTACA ACATTAGTCA GCTGGAGGAA 
TGGCTTCGGG GAAGAAACCT GCACCAGAGC GGAGCTGTTG AGACCATGGA ACCCCTGATT 
CAGGCTGCCC AGCTCCTGCA GTTAAAGAAG AAAAGCCCCG AGGATGCTGA GGCCATCTGC 
TCCCTGTGCA CCGCCCTCAG CACCCAGCAG ATTGTCAAAA TTTTGAACCT TTATACCCCT 
CTGAATGAAT TTGAAGAACG GGTGACAGTG GCTTTTATAC GAACAATCCA GGCACAACTG 
CAAGATCGGA ATGACCCTCA GCAGCTGCTA TTAGACTTCA AGCACATGTT TCCTGTTTTG 
TTCCCGTTTA ATCCATCTTC TCTGACCATG GACTCAATCC ACATCCCAGC ATGTCTCAAT 
CTGGAGTTCC TCAACGAAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315633.1
CDS	38..5521
Translation	MDALAENCFQKSSLIQYTRVWIPDPDEVWRSAELTKDYKEGDKSLQLRLEDETIQEYPIDVQNNQLPFLRNPDILVGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYDQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFTTVSGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYRIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTSQGGNTVIEGVDDAEDFEKTRQAFTLVGVRESHQISIFKIIASILHLGNVEIQAERDGESCRVSPEDEHLSDFCRLLGVELSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARDALAKHIYAQLFHWIVEHVNKALHTSLKQHSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWTQKLYDRHSGSQHFQKPRMSNKAFIVIHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDGKDSAPTATASSKINIRSSRPPVKASNKEHKKTVGYQFRSSLHLLMETLNATTPHYVRCIKPNDEKLPFHFNPKRAVQQLRACGVLETIRISAAGYPSRWSYHDFFNRYRVLVKKRDLANSDKKAICRSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRAATIMIQKAVRGWLQKVKYRRLKGATLILQRYCRGYLARRLAKHLRRTRAAVVLQKQYRMQRARRAYQRVRRAAVVIQAFARGMFVRRIYHQVLREHKATIIQKHVRGWMARRRFQRLRGAAVVIQCGFRRLKAKQALKALRIEARSAEHLKRLNVGMENKIVQLQRKIDDQNKELKTLSEQLSAITSTHTMEVEKLKKEVACYQQSQGEDRGPQLQEEVESLRTELQRAHSERKVLEDTHTREKDELRKRVADLEQENALLKNEKEQLNNQILCQSKDEFAQNSVKENLMMKKELEEERSRYQNLVKEYSRLEQRYDNLRDEMTILKQTPGHRRNPSNQSSLESDSNYPSISTSEVGDTEDTLQQVEEIGLEKAAMDMTVFLKLQKRVRELEQERKKLQVQLEKKEQQDGKRVPADQPSNDSDLDQDADLAYNSLKRQELESENKKLKNELNELRKAVADQASEHSPAAAGTPDSYSLLLNQLRVANEELEVRKEEVLILRTQIVSADQRRLAGKGSEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNRLLEAQLQAQSREHEEEVDNLKAQLEALKEEMDRQQQTFCQTLLLSPEAQVEFGIQQEMSRLTNENLDLKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSERKRHELNRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTELKPQVLAGAVPCLPAYILYMCLRHADYVNDDLKVHSLLTSTINGIKKVLKKHNEDFEMTSFWLSNTCRLLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGLLQPMIVSAMLENESIQGLSGVKPTGYRKRTSSMPEGDNSYCLEAIIRQMNSFHTVMCDQGLDPEIILQVFRQLFYMINAVTLNNLLLRKDVCSWSTGMQLRYNISQLEEWLRGRNLHQSGAVETMEPLIQAAQLLQLKKKSPEDAEAICSLCTALSTQQIVKILNLYTPLNEFEERVTVAFIRTIQAQLQDRNDPQQLLLDFKHMFPVLFPFNPSSLTMDSIHIPACLNLEFLNEV

Target ORF information:

RefSeq Version	XM_015460147.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VB (MYO5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460147.1

ORF Insert Sequence:

ATGGATGCTC TGGCTGAGAA CTGCTTTCAG AAGTCATCCC TCATTCAGTA CACAAGGGTC 
TGGATCCCTG ACCCGGATGA GGTGTGGCGC TCCGCTGAAT TAACCAAGGA CTACAAGGAG 
GGGGACAAGA GCCTCCAGCT CCGACTGGAA GATGAAACCA TTCAGGAGTA CCCCATCGAT 
GTCCAAAACA ACCAACTTCC CTTCCTGCGG AATCCAGATA TCCTGGTGGG AGAAAATGAC 
CTAACTGCCC TTAGCTATCT CCATGAGCCT GCAGTTTTGC ATAATTTAAA GGTCCGCTTC 
CTGGAGTCCA ACCATATCTA CACTTACTGT GGGATTGTGC TTGTTGCCAT TAATCCTTAT 
GATCAGTTGC CAATCTACGG TCAAGATGTC ATCTATGCCT ACAGTGGCCA GAACATGGGG 
GATATGGACC CCCACATCTT TGCTGTGGCA GAGGAAGCCT ACAAGCAGAT GGCCAGAGAT 
GAGAAGAACC AGTCCATCAT AGTCAGTGGG GAGTCTGGAG CTGGCAAGAC GGTGTCGGCC 
AAGTATGCCA TGCGCTACTT CACCACGGTC AGTGGCTCAG CCAGTGACAC CAACATCGAA 
GAGAAAGTCC TGGCGTCCAG TCCCATCATG GAGGCCATCG GGAATGCTAA AACCACCCGC 
AACGACAACA GCAGCCGTTT CGGGAAGTAC ATTCAGATCG GCTTTGACAA GAGGTACCGC 
ATCATCGGGG CCAACATGAG GACCTACCTG CTGGAGAAGT CCAGGGTGGT CTTTCAGGCA 
GACGACGAGA GGAATTACCA CATCTTTTAC CAGCTCTGTG CGGCTGCCAG TCTTCCGGAA 
TTTAAGGAGC TGGCACTAAC CTGTGCTGAG GACTTCTTCT ACACGTCACA GGGAGGAAAC 
ACAGTCATCG AGGGTGTGGA TGATGCTGAG GACTTTGAGA AGACTCGACA AGCCTTCACG 
CTCGTTGGAG TGAGAGAGTC CCATCAGATA AGCATTTTTA AGATAATTGC TTCCATCCTG 
CACCTCGGAA ATGTGGAGAT TCAAGCCGAG CGCGATGGTG AATCCTGTAG AGTATCACCT 
GAGGATGAAC ACCTAAGCGA CTTCTGCCGC CTGCTGGGCG TGGAGCTCAG TCAGATGGAG 
CACTGGCTGT GCCATCGCAA GCTGGTCACC ACCTCGGAGA CCTACGTCAA GACCATGTCC 
CTGCAGCAGG TGGTCAACGC GCGCGACGCC CTGGCCAAGC ACATCTATGC CCAGCTGTTC 
CACTGGATCG TGGAGCACGT CAACAAGGCC CTGCACACCT CCCTCAAGCA GCACTCCTTC 
ATTGGAGTCC TGGACATCTA CGGGTTTGAG ACGTTTGAGG TGAACAGCTT CGAGCAGTTC 
TGTATCAACT ACGCCAATGA GAAGCTCCAG CAGCAGTTCA ACTCACACGT ATTCAAGCTG 
GAGCAGGAGG AGTACATGAA GGAGCAGATC CCGTGGACCC TGATTGATTT CTATGATAAC 
CAGCCTTGTA TCGACCTCAT AGAAGCTAAG CTGGGCATCT TGGACCTGTT GGATGAAGAA 
TGTAAGGTCC CCAAAGGAAC CGACCAGAAC TGGACTCAGA AGCTCTATGA CAGGCACTCT 
GGCAGCCAAC ACTTCCAGAA ACCCCGCATG TCCAACAAGG CCTTCATTGT CATCCACTTT 
GCAGATAAGG TGGAGTACCT TTCAGATGGT TTTCTGGAGA AAAACAGAGA TACCGTGTAC 
GAAGAACAGA TCAACATCCT GAAGGCCAGC AAGTTCCCGC TGGTGGCTGA CTTGTTCCAT 
GATGGCAAAG ACTCTGCTCC CACCGCCACC GCTTCTTCCA AGATCAACAT TCGCTCCTCC 
AGACCCCCTG TGAAAGCCTC CAACAAGGAG CACAAGAAAA CCGTGGGTTA CCAGTTCCGC 
TCCTCCCTGC ATCTGCTCAT GGAGACACTG AACGCCACGA CACCGCACTA TGTCCGCTGC 
ATCAAGCCCA ACGACGAGAA GCTGCCCTTC CATTTTAACC CAAAGAGAGC AGTGCAGCAG 
CTCCGAGCCT GCGGCGTATT GGAGACCATC CGGATCAGCG CGGCTGGCTA CCCATCCAGG 
TGGTCCTACC ATGACTTCTT CAACCGGTAT CGGGTGCTGG TCAAGAAGAG AGACCTTGCC 
AACTCAGACA AAAAGGCCAT CTGCAGGTCT GTGCTGGAGA GCCTCATCAA GGATCCTGAC 
AAGTTCCAGT TTGGCCGCAC CAAGATCTTC TTCCGGGCCG GCCAGGTGGC CTACCTGGAG 
AAGCTTCGGG CTGACAAGTT CCGGGCGGCC ACCATCATGA TCCAGAAAGC CGTGCGGGGC 
TGGCTGCAGA AGGTGAAGTA CCGCAGGTTG AAGGGAGCCA CCTTAATTCT GCAGAGGTAC 
TGCCGAGGAT ACCTGGCCCG CAGACTGGCC AAGCATCTGC GGAGGACCCG GGCGGCCGTG 
GTGCTCCAGA AGCAATACCG CATGCAGAGG GCCCGCCGGG CCTACCAGAG GGTCCGCAGG 
GCCGCCGTCG TCATCCAGGC CTTTGCTCGG GGCATGTTTG TGCGGAGAAT CTACCACCAG 
GTCCTCAGGG AGCACAAGGC CACCATCATC CAGAAGCACG TGCGGGGCTG GATGGCTCGC 
AGGCGCTTCC AGCGGCTGCG GGGTGCGGCC GTTGTCATCC AGTGCGGCTT CCGGAGGCTC 
AAGGCCAAGC AGGCGCTGAA GGCCCTCAGG ATCGAGGCGC GCTCCGCGGA GCACCTGAAG 
CGCCTCAACG TGGGCATGGA GAACAAGATT GTCCAGCTGC AGCGGAAGAT CGATGACCAG 
AACAAAGAGC TCAAGACGCT GTCGGAGCAG CTGTCAGCAA TCACCTCCAC ACACACCATG 
GAAGTGGAGA AGCTGAAGAA GGAGGTGGCC TGCTACCAGC AGAGCCAGGG TGAGGACAGA 
GGCCCGCAGT TGCAGGAGGA GGTGGAGAGC CTGCGGACCG AGCTGCAGAG GGCCCACTCA 
GAGCGCAAGG TCCTGGAGGA CACCCACACC CGGGAGAAGG ATGAGCTGAG GAAGCGAGTT 
GCAGACCTGG AACAAGAAAA TGCACTCTTG AAAAATGAGA AGGAGCAGCT TAACAACCAG 
ATCCTGTGCC AGTCTAAAGA TGAGTTTGCC CAGAACTCTG TCAAGGAAAA CCTCATGATG 
AAGAAGGAGC TGGAGGAGGA GCGCTCCCGT TATCAGAACC TCGTGAAGGA GTATTCCCGG 
CTGGAGCAGA GATACGACAA CCTTCGGGAT GAGATGACCA TCCTAAAGCA AACCCCAGGC 
CACCGGAGGA ACCCATCAAA CCAAAGCAGC TTAGAATCTG ACTCCAATTA CCCGTCCATC 
TCCACGTCTG AGGTGGGAGA CACAGAGGAC ACCCTCCAGC AGGTGGAGGA AATTGGCCTG 
GAGAAGGCAG CCATGGACAT GACCGTCTTC CTGAAGCTGC AGAAGAGGGT GCGGGAGCTG 
GAGCAGGAGA GGAAGAAGCT GCAGGTGCAG CTGGAGAAGA AAGAACAGCA GGACGGCAAG 
AGAGTCCCGG CGGACCAACC AAGCAATGAC TCAGATTTGG ACCAGGATGC AGATCTAGCC 
TACAACAGTC TGAAGAGGCA GGAGCTGGAG TCGGAGAACA AGAAGCTGAA GAATGAGCTG 
AACGAGCTGA GGAAAGCCGT GGCCGACCAA GCCTCGGAGC ACAGCCCGGC TGCCGCCGGC 
ACCCCCGACA GCTACAGCCT CCTGCTGAAC CAGCTCCGGG TGGCCAACGA GGAGCTGGAG 
GTGCGCAAGG AGGAAGTTCT CATCCTGCGG ACCCAGATCG TGAGCGCCGA CCAGCGCCGC 
CTGGCGGGCA AGGGCTCGGA GCCAAACATC AATGCCCGAA CCAGTTGGCC GAACAGCGAG 
AAGCACGTGG ATCAGGAAGA TGCCATCGAG GCCTATCACG GGGTCTGCCA GACTAACAGG 
TTGCTGGAGG CACAGCTGCA GGCCCAGAGC CGCGAGCACG AGGAGGAGGT GGACAATCTC 
AAGGCCCAGC TGGAGGCCCT GAAGGAGGAG ATGGACAGAC AGCAGCAGAC CTTCTGCCAG 
ACCCTGCTGC TCTCCCCAGA GGCCCAGGTG GAGTTCGGCA TCCAGCAGGA GATGTCTCGG 
CTCACCAACG AGAACCTGGA CCTTAAAGAG TTAGTTGAGA AGCTGGAGAA GAACGAGAGG 
AAGCTCAAAA AGCAACTGAA GATTTACATG AAGAAGGTCC AGGACCTGGA AGCTGCCCAA 
GCCTTGGCCC AGAGCGAGAG GAAGCGGCAC GAGCTCAACA GGCAGGTCAC CGTGCAGCGG 
AAGGAGAAGG ACTTCCAAGG CATGCTGGAG TACCACAAGG AGGACGAGGC CCTGCTCATT 
CGGAATCTGG TGACAGAGTT GAAACCGCAG GTGCTGGCGG GCGCGGTGCC CTGCCTCCCA 
GCCTACATCC TCTACATGTG CCTCCGGCAC GCGGACTACG TGAACGACGA CCTCAAGGTG 
CACTCCCTGC TGACCTCCAC CATCAACGGC ATCAAGAAGG TCCTCAAGAA GCACAATGAG 
GACTTTGAGA TGACGTCGTT CTGGTTGTCC AACACCTGCC GCCTCCTGCA CTGCTTGAAG 
CAGTACAGCG GGGACGAGGG CTTCATGACT CAGAACACTG CAAAGCAGAA TGAGCACTGT 
CTTAAGAACT TTGACCTCAC TGAATACCGC CAGGTGCTGA GCGATCTTTC CATTCAGATC 
TACCAGCAGC TCATTAAAAT CGCCGAGGGC CTCTTGCAGC CTATGATAGT GTCGGCCATG 
TTGGAGAACG AGAGCATTCA GGGTCTGTCT GGGGTGAAGC CCACGGGCTA CCGGAAGCGC 
ACCTCCAGCA TGCCGGAAGG AGACAACTCG TACTGCCTGG AAGCCATCAT CCGCCAGATG 
AACTCCTTCC ACACGGTTAT GTGCGACCAG GGCCTGGACC CCGAGATCAT CCTGCAGGTG 
TTCAGACAGC TCTTCTACAT GATCAACGCG GTGACGCTCA ACAACCTGCT TCTGCGGAAG 
GACGTCTGCT CCTGGAGCAC AGGCATGCAG CTCAGGTACA ACATTAGTCA GCTGGAGGAA 
TGGCTTCGGG GAAGAAACCT GCACCAGAGC GGAGCTGTTG AGACCATGGA ACCCCTGATT 
CAGGCTGCCC AGCTCCTGCA GTTAAAGAAG AAAAGCCCCG AGGATGCTGA GGCCATCTGC 
TCCCTGTGCA CCGCCCTCAG CACCCAGCAG ATTGTCAAAA TTTTGAACCT TTATACCCCT 
CTGAATGAAT TTGAAGAACG GGTGACAGTG GCTTTTATAC GAACAATCCA GGCACAACTG 
CAAGATCGGA ATGACCCTCA GCAGCTGCTA TTAGACTTCA AGCACATGTT TCCTGTTTTG 
TTCCCGTTTA ATCCATCTTC TCTGACCATG GACTCAATCC ACATCCCAGC ATGTCTCAAT 
CTGGAGTTCC TCAACGAAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MYO5B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

サポート

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