sema3d cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	sema3d
Entrez Gene ID	100492784
Full Name	semaphorin 3D
General protein information	Preferred Names semaphorin 3D Names semaphorin-3D sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935247.4	XP_002935293.4	semaphorin-3D isoform X1
XM_018092332.1	XP_017947821.1	semaphorin-3D isoform X2
XM_002935247.5	XP_002935293.5	semaphorin-3D

Pathways from BioSystems

Homologies

	SEMA3D	XP_001107897.1
Bos taurus (cattle)	SEMA3D	NP_001193025.1
Homo sapiens (human)	SEMA3D	NP_689967.2
Canis lupus familiaris (dog)	SEMA3D	XP_533138.2
Rattus norvegicus (Norway rat)	Sema3d	NP_001098103.1
Pan troglodytes (chimpanzee)	SEMA3D	XP_527805.2
Mus musculus (house mouse)	Sema3d	NP_083158.3
Gallus gallus (chicken)	SEMA3D	NP_990704.1
Danio rerio (zebrafish)	sema3d	NP_571123.1
Xenopus tropicalis (tropical clawed frog)	sema3d	XP_002935293.2

Gene Ontology

The following sema3d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema3d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa43296	XM_002935247.4 Latest version!	Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (sema3d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OXa43297	XM_018092332.1 Latest version!	Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (sema3d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OXa54879	XM_002935247.5 Latest version!	Xenopus tropicalis semaphorin 3D (sema3d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sema3d ( XM_002935247.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935293.4 Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (sema3d), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa43296
Clone ID Related Accession (Same CDS sequence)	XM_002935247.4
Accession Version	XM_002935247.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	semaphorin-3D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935247.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCATCAAAA ATATCTCGGC AGATGGCTAA AACCGCAGCA TTCCTCTACA 
ATGTTATCAA TGGTTTTACT GATGTTTCTG CCAGCAACTG ACAGCCTGAA ACAGAATATT 
CCTCGGCTTA AGCTTTCTTA CAAAGACCTA CTTCTCTCTA ACAGCTGTGT TCCATTCCTG 
CATCCATTGA CAGAAGGCCT AGAATTTCAG ACTCTGCTGT TGGATGAAGA GAGGGGCAAG 
CTCCTTCTTG GAGCTAAAGA TCACATCTAT CTGCTTGATT TGGTAGATTT AAACAAAAAT 
CTAAGAAAGA TTCATTGGCC TGCAGCAAAA GAAAAAATTG AATTCTGCAA AAGAGCTGGT 
AAAGACAACA ATTTGGAATG TGCAAATTTC ATCAAAGTCC TTCATCATTA TAATAAAACC 
CATGCATATA CTTGTGGCAC TGGAGCATTT CATCCTCTGT GTGGATATGT TGAACTTGGA 
ACTCCCAAAG AGGAAGTGGT ATTTCAGTTA GATACACAAA ACTTGGAGTC CGGGAGGCTG 
AAATGTCCCT TTGATCCTCA ACCGCCATTC GCATCTATAA TGACAGATGA ATACCTCTTT 
GCTGGTACAG CATCAGATTT TTTAGGAAAA GAAACAACAT TTACACGTTC CATGGGACCT 
CCTCCTGACC ACCATTTTAT CAAAACCGAT ACTGATGATC ATTATTGGCT TAATGGAGCA 
AAGTTTTTTG GTGCATACGC AATACCAGAT ACATATAACC CAGATGATGA CAAGATATAC 
TTTTTCTTTC GAGAATCATC TCCAGAAGGC AGCTTTTCAG AAAAATCTGT ATTTTCTCGT 
GTAGGAAGAG TTTGCAAGAA TGACATCGGA GGTCAGCGCA GTCTGATAAA TAAATGGACG 
ACATTTCTGA AAGCTCGATT GGTTTGCTCA ATTCCTGCTC AAGATGGTGG GGATACATAT 
TTTGATGAAT TGCAAGATAT TTTCTTGCTT CCAACAAAAG ATGACAGGAA CCCAGTTGTG 
TATGGAATAT TCACTACTAC AAGTTCTATA TTCAAAGCAT CTGCTGTGTG TGTATATAGT 
ATGGCTGATA TAAGAGCTGT ATTTAATGGC CCATATGCAC ACAAGGAAAG TCCAGACCAC 
AGATGGGTAC AGTACGATGG AAGAATCCCC TATCCTCGAC CTGGAACATG TCCAAGCAAA 
ACTTATGACC CTACCATCAG ATCTACAAAA GATTTCCCTG ACGATGTTAT AAATTTTGTA 
AAAAATCACC CACTCATGTA CAAGGCTGTA TATCCACTTC ATGGCAAGCC CGTGTTCACC 
AGACTAAATG TGGACTATAA ACTGACACAG ATTGCTGTGG ATCACGTTGT AGCAGAGGAC 
GGCCAATATG ATGTCATGTT TCTTGGCACA GATATTGGAA CAGTTCTCAA GGTTATCACT 
GTGACAAAGG ATATATGGAA CATGGAGGAA GTTCTGCTGG AGGAGATGCA GATATTTAAG 
CAACCTGCCG CTATTACAAA CATAGAACTA TCACTCAAAC AGCAACAATT ATACATTACA 
TCAGAAGTTG GGTTAGTTCA ACTGTCACTG CACAGGTGTG ATACTTATGG CAAGGCTTGT 
ACGGACTGCT GCCTAGCAAG GGACCCCTAC TGTGCCTGGG ATGGAAACTC TTGTTCTCGA 
TATGTGCCAA CATCGAAAAG ACGTGCAAGA AGACAAGATG TGAAAAATGG AGATCCCCTA 
ACTCAGTGCT GGGATGTTGA AGATAACACA AATCAAGAAC CTGCAGAGGA AAAAGTAATA 
TTTGGCATTG AAAACAACTC GACTTTCCTA GAATGTATGC CTAAGTCCCA ACAAGCATCA 
ATCAGGTGGT TCATTCAACA CACTGGGGAG GAGCACCAGG AAGAGATAAC ATCTGATGAA 
AGAATCATTA AAACAGACTA CGGCCTTTTG ATTCGCAGTT TGCAGAAAAA AGACTCAGGA 
ATGTACTACT GCAAGGCTCA AGAGCACACG TTTATACACA CCATCACAAA GCTCAACGTA 
AAAATAATAG AGAATGAACA GATGGAAAAC ACACAGAAGT TGGAGGATGA TGAGGGACGG 
AGCCGTGATT TAATAACAGA GTCAAGACTG AGGTACAAAG ATTATTTACA GCTCCTCAGT 
AGTCCCAGCT TCAGTCTGGA TGAGTACTGT GAGCAGATGT GGCACAAAGA GAAGAAGAGG 
CAACGCAATA AAGGTGGTGC CAAATGGAAA CACGTCCAGG AGATTAAAAA AAACAGAAAC 
AGGAGACACC ATGAGCAGAG GAATCAGTGG GCTCGAATTT TATCAGCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935293.4
CDS	627..2957
Translation	MKEKHQKYLGRWLKPQHSSTMLSMVLLMFLPATDSLKQNIPRLKLSYKDLLLSNSCVPFLHPLTEGLEFQTLLLDEERGKLLLGAKDHIYLLDLVDLNKNLRKIHWPAAKEKIEFCKRAGKDNNLECANFIKVLHHYNKTHAYTCGTGAFHPLCGYVELGTPKEEVVFQLDTQNLESGRLKCPFDPQPPFASIMTDEYLFAGTASDFLGKETTFTRSMGPPPDHHFIKTDTDDHYWLNGAKFFGAYAIPDTYNPDDDKIYFFFRESSPEGSFSEKSVFSRVGRVCKNDIGGQRSLINKWTTFLKARLVCSIPAQDGGDTYFDELQDIFLLPTKDDRNPVVYGIFTTTSSIFKASAVCVYSMADIRAVFNGPYAHKESPDHRWVQYDGRIPYPRPGTCPSKTYDPTIRSTKDFPDDVINFVKNHPLMYKAVYPLHGKPVFTRLNVDYKLTQIAVDHVVAEDGQYDVMFLGTDIGTVLKVITVTKDIWNMEEVLLEEMQIFKQPAAITNIELSLKQQQLYITSEVGLVQLSLHRCDTYGKACTDCCLARDPYCAWDGNSCSRYVPTSKRRARRQDVKNGDPLTQCWDVEDNTNQEPAEEKVIFGIENNSTFLECMPKSQQASIRWFIQHTGEEHQEEITSDERIIKTDYGLLIRSLQKKDSGMYYCKAQEHTFIHTITKLNVKIIENEQMENTQKLEDDEGRSRDLITESRLRYKDYLQLLSSPSFSLDEYCEQMWHKEKKRQRNKGGAKWKHVQEIKKNRNRRHHEQRNQWARILSA

Target ORF information:

RefSeq Version	XM_002935247.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (sema3d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935247.4

ORF Insert Sequence:

ATGAAAGAGA AGCATCAAAA ATATCTCGGC AGATGGCTAA AACCGCAGCA TTCCTCTACA 
ATGTTATCAA TGGTTTTACT GATGTTTCTG CCAGCAACTG ACAGCCTGAA ACAGAATATT 
CCTCGGCTTA AGCTTTCTTA CAAAGACCTA CTTCTCTCTA ACAGCTGTGT TCCATTCCTG 
CATCCATTGA CAGAAGGCCT AGAATTTCAG ACTCTGCTGT TGGATGAAGA GAGGGGCAAG 
CTCCTTCTTG GAGCTAAAGA TCACATCTAT CTGCTTGATT TGGTAGATTT AAACAAAAAT 
CTAAGAAAGA TTCATTGGCC TGCAGCAAAA GAAAAAATTG AATTCTGCAA AAGAGCTGGT 
AAAGACAACA ATTTGGAATG TGCAAATTTC ATCAAAGTCC TTCATCATTA TAATAAAACC 
CATGCATATA CTTGTGGCAC TGGAGCATTT CATCCTCTGT GTGGATATGT TGAACTTGGA 
ACTCCCAAAG AGGAAGTGGT ATTTCAGTTA GATACACAAA ACTTGGAGTC CGGGAGGCTG 
AAATGTCCCT TTGATCCTCA ACCGCCATTC GCATCTATAA TGACAGATGA ATACCTCTTT 
GCTGGTACAG CATCAGATTT TTTAGGAAAA GAAACAACAT TTACACGTTC CATGGGACCT 
CCTCCTGACC ACCATTTTAT CAAAACCGAT ACTGATGATC ATTATTGGCT TAATGGAGCA 
AAGTTTTTTG GTGCATACGC AATACCAGAT ACATATAACC CAGATGATGA CAAGATATAC 
TTTTTCTTTC GAGAATCATC TCCAGAAGGC AGCTTTTCAG AAAAATCTGT ATTTTCTCGT 
GTAGGAAGAG TTTGCAAGAA TGACATCGGA GGTCAGCGCA GTCTGATAAA TAAATGGACG 
ACATTTCTGA AAGCTCGATT GGTTTGCTCA ATTCCTGCTC AAGATGGTGG GGATACATAT 
TTTGATGAAT TGCAAGATAT TTTCTTGCTT CCAACAAAAG ATGACAGGAA CCCAGTTGTG 
TATGGAATAT TCACTACTAC AAGTTCTATA TTCAAAGCAT CTGCTGTGTG TGTATATAGT 
ATGGCTGATA TAAGAGCTGT ATTTAATGGC CCATATGCAC ACAAGGAAAG TCCAGACCAC 
AGATGGGTAC AGTACGATGG AAGAATCCCC TATCCTCGAC CTGGAACATG TCCAAGCAAA 
ACTTATGACC CTACCATCAG ATCTACAAAA GATTTCCCTG ACGATGTTAT AAATTTTGTA 
AAAAATCACC CACTCATGTA CAAGGCTGTA TATCCACTTC ATGGCAAGCC CGTGTTCACC 
AGACTAAATG TGGACTATAA ACTGACACAG ATTGCTGTGG ATCACGTTGT AGCAGAGGAC 
GGCCAATATG ATGTCATGTT TCTTGGCACA GATATTGGAA CAGTTCTCAA GGTTATCACT 
GTGACAAAGG ATATATGGAA CATGGAGGAA GTTCTGCTGG AGGAGATGCA GATATTTAAG 
CAACCTGCCG CTATTACAAA CATAGAACTA TCACTCAAAC AGCAACAATT ATACATTACA 
TCAGAAGTTG GGTTAGTTCA ACTGTCACTG CACAGGTGTG ATACTTATGG CAAGGCTTGT 
ACGGACTGCT GCCTAGCAAG GGACCCCTAC TGTGCCTGGG ATGGAAACTC TTGTTCTCGA 
TATGTGCCAA CATCGAAAAG ACGTGCAAGA AGACAAGATG TGAAAAATGG AGATCCCCTA 
ACTCAGTGCT GGGATGTTGA AGATAACACA AATCAAGAAC CTGCAGAGGA AAAAGTAATA 
TTTGGCATTG AAAACAACTC GACTTTCCTA GAATGTATGC CTAAGTCCCA ACAAGCATCA 
ATCAGGTGGT TCATTCAACA CACTGGGGAG GAGCACCAGG AAGAGATAAC ATCTGATGAA 
AGAATCATTA AAACAGACTA CGGCCTTTTG ATTCGCAGTT TGCAGAAAAA AGACTCAGGA 
ATGTACTACT GCAAGGCTCA AGAGCACACG TTTATACACA CCATCACAAA GCTCAACGTA 
AAAATAATAG AGAATGAACA GATGGAAAAC ACACAGAAGT TGGAGGATGA TGAGGGACGG 
AGCCGTGATT TAATAACAGA GTCAAGACTG AGGTACAAAG ATTATTTACA GCTCCTCAGT 
AGTCCCAGCT TCAGTCTGGA TGAGTACTGT GAGCAGATGT GGCACAAAGA GAAGAAGAGG 
CAACGCAATA AAGGTGGTGC CAAATGGAAA CACGTCCAGG AGATTAAAAA AAACAGAAAC 
AGGAGACACC ATGAGCAGAG GAATCAGTGG GCTCGAATTT TATCAGCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3d ( XM_018092332.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947821.1 Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (sema3d), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa43297
Clone ID Related Accession (Same CDS sequence)	XM_018092332.1
Accession Version	XM_018092332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	semaphorin-3D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCATCAAAA ATATCTCGGC AGATGGCTAA AACCGCAGCA TTCCTCTACA 
ATGTTATCAA TGGTTTTACT GATGTTTCTG CCAGCAACTG ACAGCCTGAA ACAGAATATT 
CCTCGGCTTA AGCTTTCTTA CAAAGACCTA CTTCTCTCTA ACAGCTGTGT TCCATTCCTG 
CATCCATTGA CAGAAGGCCT AGAATTTCAG ACTCTGCTGT TGGATGAAGA GAGGGGCAAG 
CTCCTTCTTG GAGCTAAAGA TCACATCTAT CTGCTTGATT TGGTAGATTT AAACAAAAAT 
CTAAGAAAGA TTCATTGGCC TGCAGCAAAA GAAAAAATTG AATTCTGCAA AAGAGCTGGT 
AAAGACAACA ATTTGGAATG TGCAAATTTC ATCAAAGTCC TTCATCATTA TAATAAAACC 
CATGCATATA CTTGTGGCAC TGGAGCATTT CATCCTCTGT GTGGATATGT TGAACTTGGA 
ACTCCCAAAG AGGAAGTGGT ATTTCAGTTA GATACACAAA ACTTGGAGTC CGGGAGGCTG 
AAATGTCCCT TTGATCCTCA ACCGCCATTC GCATCTATAA TGACAGATGA ATACCTCTTT 
GCTGGTACAG CATCAGATTT TTTAGGAAAA GAAACAACAT TTACACGTTC CATGGGACCT 
CCTCCTGACC ACCATTTTAT CAAAACCGAT ACTGATGATC ATTATTGGCT TAATGGAGCA 
AAGTTTTTTG GTGCATACGC AATACCAGAT ACATATAACC CAGATGATGA CAAGATATAC 
TTTTTCTTTC GAGAATCATC TCCAGAAGGC AGCTTTTCAG AAAAATCTGT ATTTTCTCGT 
GTAGGAAGAG TTTGCAAGAA TGACATCGGA GGTCAGCGCA GTCTGATAAA TAAATGGACG 
ACATTTCTGA AAGCTCGATT GGTTTGCTCA ATTCCTGCTC AAGATGGTGG GGATACATAT 
TTTGATGAAT TGCAAGATAT TTTCTTGCTT CCAACAAAAG ATGACAGGAA CCCAGTTGTG 
TATGGAATAT TCACTACTAC AAGTTCTATA TTCAAAGCAT CTGCTGTGTG TGTATATAGT 
ATGGCTGATA TAAGAGCTGT ATTTAATGGC CCATATGCAC ACAAGGAAAG TCCAGACCAC 
AGATGGGTAC AGTACGATGG AAGAATCCCC TATCCTCGAC CTGGAACATG TCCAAGCAAA 
ACTTATGACC CTACCATCAG ATCTACAAAA GATTTCCCTG ACGATGTTAT AAATTTTGTA 
AAAAATCACC CACTCATGTA CAAGGCTGTA TATCCACTTC ATGGCAAGCC CGTGTTCACC 
AGACTAAATG TGGACTATAA ACTGACACAG ATTGCTGTGG ATCACGTTGT AGCAGAGGAC 
GGCCAATATG ATGTCATGTT TCTTGGCACA GATATTGGAA CAGTTCTCAA GGTTATCACT 
GTGACAAAGG ATATATGGAA CATGGAGGAA GTTCTGCTGG AGGAGATGCA GATATTTAAG 
CAACAATTAT ACATTACATC AGAAGTTGGG TTAGTTCAAC TGTCACTGCA CAGGTGTGAT 
ACTTATGGCA AGGCTTGTAC GGACTGCTGC CTAGCAAGGG ACCCCTACTG TGCCTGGGAT 
GGAAACTCTT GTTCTCGATA TGTGCCAACA TCGAAAAGAC GTGCAAGAAG ACAAGATGTG 
AAAAATGGAG ATCCCCTAAC TCAGTGCTGG GATGTTGAAG ATAACACAAA TCAAGAACCT 
GCAGAGGAAA AAGTAATATT TGGCATTGAA AACAACTCGA CTTTCCTAGA ATGTATGCCT 
AAGTCCCAAC AAGCATCAAT CAGGTGGTTC ATTCAACACA CTGGGGAGGA GCACCAGGAA 
GAGATAACAT CTGATGAAAG AATCATTAAA ACAGACTACG GCCTTTTGAT TCGCAGTTTG 
CAGAAAAAAG ACTCAGGAAT GTACTACTGC AAGGCTCAAG AGCACACGTT TATACACACC 
ATCACAAAGC TCAACGTAAA AATAATAGAG AATGAACAGA TGGAAAACAC ACAGAAGTTG 
GAGGATGATG AGGGACGGAG CCGTGATTTA ATAACAGAGT CAAGACTGAG GTACAAAGAT 
TATTTACAGC TCCTCAGTAG TCCCAGCTTC AGTCTGGATG AGTACTGTGA GCAGATGTGG 
CACAAAGAGA AGAAGAGGCA ACGCAATAAA GGTGGTGCCA AATGGAAACA CGTCCAGGAG 
ATTAAAAAAA ACAGAAACAG GAGACACCAT GAGCAGAGGA ATCAGTGGGC TCGAATTTTA 
TCAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947821.1
CDS	629..2917
Translation	MKEKHQKYLGRWLKPQHSSTMLSMVLLMFLPATDSLKQNIPRLKLSYKDLLLSNSCVPFLHPLTEGLEFQTLLLDEERGKLLLGAKDHIYLLDLVDLNKNLRKIHWPAAKEKIEFCKRAGKDNNLECANFIKVLHHYNKTHAYTCGTGAFHPLCGYVELGTPKEEVVFQLDTQNLESGRLKCPFDPQPPFASIMTDEYLFAGTASDFLGKETTFTRSMGPPPDHHFIKTDTDDHYWLNGAKFFGAYAIPDTYNPDDDKIYFFFRESSPEGSFSEKSVFSRVGRVCKNDIGGQRSLINKWTTFLKARLVCSIPAQDGGDTYFDELQDIFLLPTKDDRNPVVYGIFTTTSSIFKASAVCVYSMADIRAVFNGPYAHKESPDHRWVQYDGRIPYPRPGTCPSKTYDPTIRSTKDFPDDVINFVKNHPLMYKAVYPLHGKPVFTRLNVDYKLTQIAVDHVVAEDGQYDVMFLGTDIGTVLKVITVTKDIWNMEEVLLEEMQIFKQQLYITSEVGLVQLSLHRCDTYGKACTDCCLARDPYCAWDGNSCSRYVPTSKRRARRQDVKNGDPLTQCWDVEDNTNQEPAEEKVIFGIENNSTFLECMPKSQQASIRWFIQHTGEEHQEEITSDERIIKTDYGLLIRSLQKKDSGMYYCKAQEHTFIHTITKLNVKIIENEQMENTQKLEDDEGRSRDLITESRLRYKDYLQLLSSPSFSLDEYCEQMWHKEKKRQRNKGGAKWKHVQEIKKNRNRRHHEQRNQWARILSA

Target ORF information:

RefSeq Version	XM_018092332.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (sema3d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092332.1

ORF Insert Sequence:

ATGAAAGAGA AGCATCAAAA ATATCTCGGC AGATGGCTAA AACCGCAGCA TTCCTCTACA 
ATGTTATCAA TGGTTTTACT GATGTTTCTG CCAGCAACTG ACAGCCTGAA ACAGAATATT 
CCTCGGCTTA AGCTTTCTTA CAAAGACCTA CTTCTCTCTA ACAGCTGTGT TCCATTCCTG 
CATCCATTGA CAGAAGGCCT AGAATTTCAG ACTCTGCTGT TGGATGAAGA GAGGGGCAAG 
CTCCTTCTTG GAGCTAAAGA TCACATCTAT CTGCTTGATT TGGTAGATTT AAACAAAAAT 
CTAAGAAAGA TTCATTGGCC TGCAGCAAAA GAAAAAATTG AATTCTGCAA AAGAGCTGGT 
AAAGACAACA ATTTGGAATG TGCAAATTTC ATCAAAGTCC TTCATCATTA TAATAAAACC 
CATGCATATA CTTGTGGCAC TGGAGCATTT CATCCTCTGT GTGGATATGT TGAACTTGGA 
ACTCCCAAAG AGGAAGTGGT ATTTCAGTTA GATACACAAA ACTTGGAGTC CGGGAGGCTG 
AAATGTCCCT TTGATCCTCA ACCGCCATTC GCATCTATAA TGACAGATGA ATACCTCTTT 
GCTGGTACAG CATCAGATTT TTTAGGAAAA GAAACAACAT TTACACGTTC CATGGGACCT 
CCTCCTGACC ACCATTTTAT CAAAACCGAT ACTGATGATC ATTATTGGCT TAATGGAGCA 
AAGTTTTTTG GTGCATACGC AATACCAGAT ACATATAACC CAGATGATGA CAAGATATAC 
TTTTTCTTTC GAGAATCATC TCCAGAAGGC AGCTTTTCAG AAAAATCTGT ATTTTCTCGT 
GTAGGAAGAG TTTGCAAGAA TGACATCGGA GGTCAGCGCA GTCTGATAAA TAAATGGACG 
ACATTTCTGA AAGCTCGATT GGTTTGCTCA ATTCCTGCTC AAGATGGTGG GGATACATAT 
TTTGATGAAT TGCAAGATAT TTTCTTGCTT CCAACAAAAG ATGACAGGAA CCCAGTTGTG 
TATGGAATAT TCACTACTAC AAGTTCTATA TTCAAAGCAT CTGCTGTGTG TGTATATAGT 
ATGGCTGATA TAAGAGCTGT ATTTAATGGC CCATATGCAC ACAAGGAAAG TCCAGACCAC 
AGATGGGTAC AGTACGATGG AAGAATCCCC TATCCTCGAC CTGGAACATG TCCAAGCAAA 
ACTTATGACC CTACCATCAG ATCTACAAAA GATTTCCCTG ACGATGTTAT AAATTTTGTA 
AAAAATCACC CACTCATGTA CAAGGCTGTA TATCCACTTC ATGGCAAGCC CGTGTTCACC 
AGACTAAATG TGGACTATAA ACTGACACAG ATTGCTGTGG ATCACGTTGT AGCAGAGGAC 
GGCCAATATG ATGTCATGTT TCTTGGCACA GATATTGGAA CAGTTCTCAA GGTTATCACT 
GTGACAAAGG ATATATGGAA CATGGAGGAA GTTCTGCTGG AGGAGATGCA GATATTTAAG 
CAACAATTAT ACATTACATC AGAAGTTGGG TTAGTTCAAC TGTCACTGCA CAGGTGTGAT 
ACTTATGGCA AGGCTTGTAC GGACTGCTGC CTAGCAAGGG ACCCCTACTG TGCCTGGGAT 
GGAAACTCTT GTTCTCGATA TGTGCCAACA TCGAAAAGAC GTGCAAGAAG ACAAGATGTG 
AAAAATGGAG ATCCCCTAAC TCAGTGCTGG GATGTTGAAG ATAACACAAA TCAAGAACCT 
GCAGAGGAAA AAGTAATATT TGGCATTGAA AACAACTCGA CTTTCCTAGA ATGTATGCCT 
AAGTCCCAAC AAGCATCAAT CAGGTGGTTC ATTCAACACA CTGGGGAGGA GCACCAGGAA 
GAGATAACAT CTGATGAAAG AATCATTAAA ACAGACTACG GCCTTTTGAT TCGCAGTTTG 
CAGAAAAAAG ACTCAGGAAT GTACTACTGC AAGGCTCAAG AGCACACGTT TATACACACC 
ATCACAAAGC TCAACGTAAA AATAATAGAG AATGAACAGA TGGAAAACAC ACAGAAGTTG 
GAGGATGATG AGGGACGGAG CCGTGATTTA ATAACAGAGT CAAGACTGAG GTACAAAGAT 
TATTTACAGC TCCTCAGTAG TCCCAGCTTC AGTCTGGATG AGTACTGTGA GCAGATGTGG 
CACAAAGAGA AGAAGAGGCA ACGCAATAAA GGTGGTGCCA AATGGAAACA CGTCCAGGAG 
ATTAAAAAAA ACAGAAACAG GAGACACCAT GAGCAGAGGA ATCAGTGGGC TCGAATTTTA 
TCAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3d ( XM_002935247.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935293.5 Xenopus tropicalis semaphorin 3D (sema3d), mRNA.

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CloneID	OXa54879
Clone ID Related Accession (Same CDS sequence)	XM_002935247.5
Accession Version	XM_002935247.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	semaphorin-3D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935247.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCATCAAAA ATATCTCGGC AGATGGCTAA AACCGCAGCA TTCCTCTACA 
ATGTTATCAA TGGTTTTACT GATGTTTCTG CCAGCAACTG ACAGCCTGAA ACAGAATATT 
CCTCGGCTTA AGCTTTCTTA CAAAGACCTA CTTCTCTCTA ACAGCTGTGT TCCATTCCTG 
CATCCATTGA CAGAAGGCCT AGAATTTCAG ACTCTGCTGT TGGATGAAGA GAGGGGCAAG 
CTCCTTCTTG GAGCTAAAGA TCACATCTAT CTGCTTGATT TGGTAGATTT AAACAAAAAT 
CTAAGAAAGA TTCATTGGCC CGCAGCAAAA GAAAAAATTG AATTCTGCAA AAGAGCTGGT 
AAAGACAACA ATTTGGAATG TGCAAATTTC ATCAAAGTCC TTCATCATTA TAATAAAACC 
CATGCATATA CTTGTGGCAC TGGAGCATTT CATCCTCTGT GTGGATATGT TGAACTTGGA 
ACTCCCAAAG AGGAAGTGGT ATTTCAGTTA GATACACAAA ACTTGGAGTC CGGGAGGCTG 
AAATGTCCCT TTGATCCTCA ACCGCCATTC GCATCTATAA TGACAGATGA ATACCTCTTT 
GCTGGTACAG CATCAGATTT TTTAGGAAAA GAAACAACAT TTACACGTTC CATGGGACCT 
CCTCCTGACC ACCATTTTAT CAAAACTGAT ACTGATGATC ATTATTGGCT TAATGGAGCA 
AAGTTTTTTG GTGCATACGC AATACCAGAT ACATATAACC CAGATGATGA CAAGATATAC 
TTTTTCTTTC GAGAATCATC TCCAGAAGGC AGCTTTTCAG AAAAATCTGT ATTTTCTCGT 
GTAGGAAGAG TTTGCAAGAA TGACATCGGA GGTCAGCGCA GTCTGATAAA TAAATGGACG 
ACATTTCTGA AAGCTCGATT GGTTTGCTCA ATTCCTGCTC AAGATGGTGG GGATACATAT 
TTTGATGAAT TGCAAGATAT TTTCTTGCTT CCAACAAAAG ATGAAAGGAA CCCAGTTGTG 
TATGGAATAT TCACTACTAC AAGTTCTATA TTCAAAGCAT CTGCTGTGTG TGTATATAGT 
ATGGCTGATA TAAGAGCTGT ATTTAATGGC CCATATGCAC ACAAGGAAAG TCCAGACCAC 
AGATGGGTAC AGTACGATGG AAGAATCCCC TATCCTCGAC CTGGAACATG TCCAAGCAAA 
ACTTATGACC CTACCATCAG ATCTACAAAA GATTTCCCTG ACGATGTTAT AAATTTTGTA 
AAAAATCACC CACTCATGTA CAAGGCTGTA TATCCACTTC ATGGCAAGCC CGTGTTCACC 
AGACTAAATG TGGACTATAA ACTGACACAG ATTGCTGTGG ATCACGTTGT AGCAGAGGAC 
GGCCAATATG ATGTCATGTT TCTTGGCACA GATATTGGAA CAGTTCTCAA GGTTATCACT 
GTGACAAAGG ATATATGGAA CATGGAGGAA GTTCTGCTGG AGGAGATGCA GATATTTAAG 
CAACCTGCCG CTATCACAAA CATAGAACTA TCACTCAAAC AGCAACAATT ATACATTACA 
TCAGAAGTTG GGTTAGTTCA ACTGTCACTG CACAGGTGTG ATACTTATGG CAAGGCTTGT 
ACGGACTGCT GCCTAGCAAG GGACCCCTAC TGTGCCTGGG ATGGAAACTC TTGTTCTCGA 
TATGTGCCAA CATCGAAAAG ACGTGCAAGA AGACAAGATG TGAAAAATGG AGATCCCCTA 
ACTCAGTGCT GGGATGTTGA AGATAACACA AATCAAGAAC CTGCAGAGGA AAAAGTAATA 
TTTGGCATTG AAAACAACTC GACTTTCCTA GAATGTATGC CTAAGTCCCA ACAAGCATCA 
ATCAGGTGGT TCATTCAACA CACTGGGGAG GAGCACCAGG AAGAGATAAC ATCTGATGAA 
AGAATCATTA AAACAGACTA CGGCCTTTTG ATTCGCAGTT TGCAGAAAAA AGACTCAGGA 
ATGTACTACT GCAAGGCTCA AGAGCACACG TTTATACACA CCATCACAAA GCTCAACGTA 
AAAATAATAG AGAATGAACA GATGGAAAAC ACACAGAAGT TGGAGGATGA TGAGGGACGG 
AGCCGTGATT TAATAACAGA GTCAAGACTG AGGTACAAAG ATTATTTACA GCTCCTCAGT 
AGTCCCAGCT TCAGTCTGGA TGAGTACTGT GAGCAGATGT GGCACAAAGA GAAGAAGAGG 
CAACGCAATA AAGGTGGCGC CAAATGGAAA CACGTCCAGG AGATTAAAAA AAACAGAAAC 
AGGAGACACC ATGAGCAGAG GAATCAGTGG GCTCGAATTT TATCAGCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935293.5
CDS	528..2858
Translation	MKEKHQKYLGRWLKPQHSSTMLSMVLLMFLPATDSLKQNIPRLKLSYKDLLLSNSCVPFLHPLTEGLEFQTLLLDEERGKLLLGAKDHIYLLDLVDLNKNLRKIHWPAAKEKIEFCKRAGKDNNLECANFIKVLHHYNKTHAYTCGTGAFHPLCGYVELGTPKEEVVFQLDTQNLESGRLKCPFDPQPPFASIMTDEYLFAGTASDFLGKETTFTRSMGPPPDHHFIKTDTDDHYWLNGAKFFGAYAIPDTYNPDDDKIYFFFRESSPEGSFSEKSVFSRVGRVCKNDIGGQRSLINKWTTFLKARLVCSIPAQDGGDTYFDELQDIFLLPTKDERNPVVYGIFTTTSSIFKASAVCVYSMADIRAVFNGPYAHKESPDHRWVQYDGRIPYPRPGTCPSKTYDPTIRSTKDFPDDVINFVKNHPLMYKAVYPLHGKPVFTRLNVDYKLTQIAVDHVVAEDGQYDVMFLGTDIGTVLKVITVTKDIWNMEEVLLEEMQIFKQPAAITNIELSLKQQQLYITSEVGLVQLSLHRCDTYGKACTDCCLARDPYCAWDGNSCSRYVPTSKRRARRQDVKNGDPLTQCWDVEDNTNQEPAEEKVIFGIENNSTFLECMPKSQQASIRWFIQHTGEEHQEEITSDERIIKTDYGLLIRSLQKKDSGMYYCKAQEHTFIHTITKLNVKIIENEQMENTQKLEDDEGRSRDLITESRLRYKDYLQLLSSPSFSLDEYCEQMWHKEKKRQRNKGGAKWKHVQEIKKNRNRRHHEQRNQWARILSA

Target ORF information:

RefSeq Version	XM_002935247.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis semaphorin 3D (sema3d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935247.5

ORF Insert Sequence:

ATGAAAGAGA AGCATCAAAA ATATCTCGGC AGATGGCTAA AACCGCAGCA TTCCTCTACA 
ATGTTATCAA TGGTTTTACT GATGTTTCTG CCAGCAACTG ACAGCCTGAA ACAGAATATT 
CCTCGGCTTA AGCTTTCTTA CAAAGACCTA CTTCTCTCTA ACAGCTGTGT TCCATTCCTG 
CATCCATTGA CAGAAGGCCT AGAATTTCAG ACTCTGCTGT TGGATGAAGA GAGGGGCAAG 
CTCCTTCTTG GAGCTAAAGA TCACATCTAT CTGCTTGATT TGGTAGATTT AAACAAAAAT 
CTAAGAAAGA TTCATTGGCC CGCAGCAAAA GAAAAAATTG AATTCTGCAA AAGAGCTGGT 
AAAGACAACA ATTTGGAATG TGCAAATTTC ATCAAAGTCC TTCATCATTA TAATAAAACC 
CATGCATATA CTTGTGGCAC TGGAGCATTT CATCCTCTGT GTGGATATGT TGAACTTGGA 
ACTCCCAAAG AGGAAGTGGT ATTTCAGTTA GATACACAAA ACTTGGAGTC CGGGAGGCTG 
AAATGTCCCT TTGATCCTCA ACCGCCATTC GCATCTATAA TGACAGATGA ATACCTCTTT 
GCTGGTACAG CATCAGATTT TTTAGGAAAA GAAACAACAT TTACACGTTC CATGGGACCT 
CCTCCTGACC ACCATTTTAT CAAAACTGAT ACTGATGATC ATTATTGGCT TAATGGAGCA 
AAGTTTTTTG GTGCATACGC AATACCAGAT ACATATAACC CAGATGATGA CAAGATATAC 
TTTTTCTTTC GAGAATCATC TCCAGAAGGC AGCTTTTCAG AAAAATCTGT ATTTTCTCGT 
GTAGGAAGAG TTTGCAAGAA TGACATCGGA GGTCAGCGCA GTCTGATAAA TAAATGGACG 
ACATTTCTGA AAGCTCGATT GGTTTGCTCA ATTCCTGCTC AAGATGGTGG GGATACATAT 
TTTGATGAAT TGCAAGATAT TTTCTTGCTT CCAACAAAAG ATGAAAGGAA CCCAGTTGTG 
TATGGAATAT TCACTACTAC AAGTTCTATA TTCAAAGCAT CTGCTGTGTG TGTATATAGT 
ATGGCTGATA TAAGAGCTGT ATTTAATGGC CCATATGCAC ACAAGGAAAG TCCAGACCAC 
AGATGGGTAC AGTACGATGG AAGAATCCCC TATCCTCGAC CTGGAACATG TCCAAGCAAA 
ACTTATGACC CTACCATCAG ATCTACAAAA GATTTCCCTG ACGATGTTAT AAATTTTGTA 
AAAAATCACC CACTCATGTA CAAGGCTGTA TATCCACTTC ATGGCAAGCC CGTGTTCACC 
AGACTAAATG TGGACTATAA ACTGACACAG ATTGCTGTGG ATCACGTTGT AGCAGAGGAC 
GGCCAATATG ATGTCATGTT TCTTGGCACA GATATTGGAA CAGTTCTCAA GGTTATCACT 
GTGACAAAGG ATATATGGAA CATGGAGGAA GTTCTGCTGG AGGAGATGCA GATATTTAAG 
CAACCTGCCG CTATCACAAA CATAGAACTA TCACTCAAAC AGCAACAATT ATACATTACA 
TCAGAAGTTG GGTTAGTTCA ACTGTCACTG CACAGGTGTG ATACTTATGG CAAGGCTTGT 
ACGGACTGCT GCCTAGCAAG GGACCCCTAC TGTGCCTGGG ATGGAAACTC TTGTTCTCGA 
TATGTGCCAA CATCGAAAAG ACGTGCAAGA AGACAAGATG TGAAAAATGG AGATCCCCTA 
ACTCAGTGCT GGGATGTTGA AGATAACACA AATCAAGAAC CTGCAGAGGA AAAAGTAATA 
TTTGGCATTG AAAACAACTC GACTTTCCTA GAATGTATGC CTAAGTCCCA ACAAGCATCA 
ATCAGGTGGT TCATTCAACA CACTGGGGAG GAGCACCAGG AAGAGATAAC ATCTGATGAA 
AGAATCATTA AAACAGACTA CGGCCTTTTG ATTCGCAGTT TGCAGAAAAA AGACTCAGGA 
ATGTACTACT GCAAGGCTCA AGAGCACACG TTTATACACA CCATCACAAA GCTCAACGTA 
AAAATAATAG AGAATGAACA GATGGAAAAC ACACAGAAGT TGGAGGATGA TGAGGGACGG 
AGCCGTGATT TAATAACAGA GTCAAGACTG AGGTACAAAG ATTATTTACA GCTCCTCAGT 
AGTCCCAGCT TCAGTCTGGA TGAGTACTGT GAGCAGATGT GGCACAAAGA GAAGAAGAGG 
CAACGCAATA AAGGTGGCGC CAAATGGAAA CACGTCCAGG AGATTAAAAA AAACAGAAAC 
AGGAGACACC ATGAGCAGAG GAATCAGTGG GCTCGAATTT TATCAGCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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sema3d cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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