SEMA3D cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEMA3D
Entrez Gene ID	223117
Full Name	semaphorin 3D
Synonyms	Sema-Z2,coll-2
General protein information	Preferred Names semaphorin 3D Names semaphorin-3D collapsin 2 sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q21.11
Summary	This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin like domain and a C-terminal basic domain. The protein encoded by this gene binds neuropilin and plays an important role in cardiovascular development. [provided by RefSeq, Aug 2016].
Disorder MIM:	609907

mRNA and Protein(s)

mRNA	Protein	Name
XM_011515961.2	XP_011514263.1	semaphorin-3D isoform X2
NM_152754.2	NP_689967.2	semaphorin-3D precursor
XM_017011873.1	XP_016867362.1	semaphorin-3D isoform X1
XM_011515960.1	XP_011514262.1	semaphorin-3D isoform X1

Pathways from BioSystems

Homologies

	SEMA3D	XP_001107897.1
Bos taurus (cattle)	SEMA3D	NP_001193025.1
Homo sapiens (human)	SEMA3D	NP_689967.2
Canis lupus familiaris (dog)	SEMA3D	XP_533138.2
Rattus norvegicus (Norway rat)	Sema3d	NP_001098103.1
Pan troglodytes (chimpanzee)	SEMA3D	XP_527805.2
Mus musculus (house mouse)	Sema3d	NP_083158.3
Gallus gallus (chicken)	SEMA3D	NP_990704.1
Danio rerio (zebrafish)	sema3d	NP_571123.1
Xenopus tropicalis (tropical clawed frog)	sema3d	XP_002935293.2

Gene Ontology
Bibliography

Related articles in PubMed

Promoter-level transcriptome in primary lesions of endometrial cancer identified biomarkers associated with lymph node metastasis.
Yoshida E, Terao Y, Hayashi N, Mogushi K, Arakawa A, Tanaka Y, Ito Y, Ohmiya H, Hayashizaki Y, Takeda S, Itoh M, Kawaji H
Scientific reports7(1)14160(2017 Oct)

Decreased expression of semaphorin 3D is associated with genesis and development in colorectal cancer.
Wang Z, Ding M, Qian N, Song B, Yu J, Tang J, Wang J
World journal of surgical oncology15(1)67(2017 Mar)

Variable expressivity and genetic heterogeneity involving DPT and SEMA3D genes in autosomal dominant familial Meniere's disease.
Mart?n-Sierra C, Gallego-Martinez A, Requena T, Frejo L, Batuecas-Caletr?o A, Lopez-Escamez JA
European journal of human genetics : EJHG25(2)200-207(2017 02)

CTNNA3 and SEMA3D: Promising loci for asthma exacerbation identified through multiple genome-wide association studies.
McGeachie MJ, Wu AC, Tse SM, Clemmer GL, Sordillo J, Himes BE, Lasky-Su J, Chase RP, Martinez FD, Weeke P, Shaffer CM, Xu H, Denny JC, Roden DM, Panettieri RA Jr, Raby BA, Weiss ST, Tantisira KG
The Journal of allergy and clinical immunology136(6)1503-1510(2015 Dec)

Class 3 semaphorins expression and association with innervation and angiogenesis within the degenerate human intervertebral disc.
Binch AL, Cole AA, Breakwell LM, Michael AL, Chiverton N, Creemers LB, Cross AK, Le Maitre CL
Oncotarget6(21)18338-54(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Low SEMA3D expression is associated with colorectal cancer.
Title: Decreased expression of semaphorin 3D is associated with genesis and development in colorectal cancer.

Novel mutation in SEMA3D segregates with the complete phenotype with variable expressivity in two pedigrees with autosomal dominant familial Meniere's disease.
Title: Variable expressivity and genetic heterogeneity involving DPT and SEMA3D genes in autosomal dominant familial Meniere's disease.

CTNNA3 and SEMA3D: Promising loci for asthma exacerbation identified through multiple genome-wide association studies
Title: CTNNA3 and SEMA3D: Promising loci for asthma exacerbation identified through multiple genome-wide association studies.

Data show significant increases in semaphorin3C, 3D and their receptor neuropilin-2 in degenerate samples which were shown to contain nerves and blood vessels, compared to non-degenerate samples without nerves and blood vessels.
Title: Class 3 semaphorins expression and association with innervation and angiogenesis within the degenerate human intervertebral disc.

Functional loss of semaphorin 3C and/or semaphorin 3D and their epistatic interaction with ret are critical to Hirschsprung disease liability.
Title: Functional loss of semaphorin 3C and/or semaphorin 3D and their epistatic interaction with ret are critical to Hirschsprung disease liability.

The following SEMA3D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA3D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06915	NM_152754.2 Latest version!	Homo sapiens semaphorin 3D (SEMA3D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu06915	XM_017011873.1 Latest version!	Homo sapiens semaphorin 3D (SEMA3D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu06915	XM_011515960.1 Latest version!	Homo sapiens semaphorin 3D (SEMA3D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu78152	XM_011515961.2 Latest version!	Homo sapiens semaphorin 3D (SEMA3D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEMA3D ( NM_152754.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_689967.2 Homo sapiens semaphorin 3D (SEMA3D), mRNA.

Price & Availability↑

CloneID	OHu06915
Clone ID Related Accession (Same CDS sequence)	NM_152754.2 , XM_017011873.1 , XM_011515960.1
Accession Version	NM_152754.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	semaphorin-3D precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY358937.1, BC029590.1 and BX648652.1. This sequence is a reference standard in the RefSeqGene project. On Jan 30, 2004 this sequence version replaced NM_152754.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY358937.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATGCTA ATAAAGATGA AAGACTTAAA GCCAGAAGCC AAGATTTTCA CCTTTTTCCT 
GCTTTGATGA TGCTAAGCAT GACCATGTTG TTTCTTCCAG TCACTGGCAC TTTGAAGCAA 
AATATTCCAA GACTCAAGCT AACCTACAAA GACTTGCTGC TTTCAAATAG CTGTATTCCC 
TTTTTGGGTT CATCAGAAGG ACTGGATTTT CAAACTCTTC TCTTAGATGA GGAAAGAGGC 
AGGCTGCTCT TGGGAGCCAA AGACCACATC TTTCTACTCA GTCTGGTTGA CTTAAACAAA 
AATTTTAAGA AGATTTATTG GCCTGCTGCA AAGGAACGGG TGGAATTATG TAAATTAGCT 
GGGAAAGATG CCAATACAGA ATGTGCAAAT TTCATCAGAG TACTTCAGCC CTATAACAAA 
ACTCACATAT ATGTGTGTGG AACTGGAGCA TTTCATCCAA TATGTGGGTA TATTGATCTT 
GGAGTCTACA AGGAGGATAT TATATTCAAA CTAGACACAC ATAATTTGGA GTCTGGCAGA 
CTGAAATGTC CTTTCGATCC TCAGCAGCCT TTTGCTTCAG TAATGACAGA TGAGTACCTC 
TACTCTGGAA CAGCTTCTGA TTTCCTTGGC AAAGATACTG CATTCACTCG ATCCCTTGGG 
CCTACTCATG ACCACCACTA CATCAGAACT GACATTTCAG AGCACTACTG GCTCAATGGA 
GCAAAATTTA TTGGAACTTT CTTCATACCA GACACCTACA ATCCAGATGA TGATAAAATA 
TATTTCTTCT TTCGTGAATC ATCTCAAGAA GGCAGTACCT CCGATAAAAC CATCCTTTCT 
CGAGTTGGAA GAGTTTGTAA GAATGATGTA GGAGGACAAC GCAGCCTGAT AAACAAGTGG 
ACGACTTTTC TTAAGGCCAG ACTGATTTGC TCAATTCCTG GAAGTGATGG GGCAGATACT 
TACTTTGATG AGCTTCAAGA TATTTATTTA CTCCCCACAA GAGATGAAAG AAATCCTGTA 
GTATATGGAG TCTTTACTAC AACCAGCTCC ATCTTCAAAG GCTCTGCTGT TTGTGTGTAT 
AGCATGGCTG ACATCAGAGC AGTTTTTAAT GGTCCATATG CTCATAAGGA AAGTGCAGAC 
CATCGTTGGG TGCAGTATGA TGGGAGAATT CCTTATCCAC GGCCTGGTAC ATGTCCAAGC 
AAAACCTATG ACCCACTGAT TAAGTCCACC CGAGATTTTC CAGATGATGT CATCAGTTTC 
ATAAAGCGGC ACTCTGTGAT GTATAAGTCC GTATACCCAG TTGCAGGAGG ACCAACGTTC 
AAGAGAATCA ATGTGGATTA CAGACTGACA CAGATAGTGG TGGATCATGT CATTGCAGAA 
GATGGCCAGT ACGATGTAAT GTTTCTTGGA ACAGACATTG GAACTGTCCT CAAAGTTGTC 
AGCATTTCAA AGGAAAAGTG GAATATGGAA GAGGTAGTGC TGGAGGAGTT GCAGATATTC 
AAGCACTCAT CAATCATCTT GAACATGGAA TTGTCTCTGA AGCAGCAACA ATTGTACATT 
GGTTCCCGAG ATGGATTGGT TCAGCTCTCC TTGCACAGAT GCGACACTTA TGGGAAAGCT 
TGCGCAGACT GTTGTCTTGC CAGAGACCCC TACTGTGCCT GGGATGGAAA TGCATGCTCT 
CGATATGCTC CTACTTCTAA AAGGAGAGCT AGACGCCAAG ATGTAAAATA TGGCGACCCA 
ATCACCCAGT GCTGGGACAT CGAAGACAGC ATTAGTCATG AAACTGCTGA TGAAAAGGTG 
ATTTTTGGCA TTGAATTTAA CTCAACCTTT CTGGAATGTA TACCTAAATC CCAACAAGCA 
ACTATTAAAT GGTATATCCA GAGGTCAGGG GATGAGCATC GAGAGGAGTT GAAGCCCGAT 
GAAAGAATCA TCAAAACGGA ATATGGGCTA CTGATTCGAA GTTTGCAGAA GAAGGATTCT 
GGGATGTATT ACTGCAAAGC CCAGGAGCAC ACTTTCATCC ACACCATAGT GAAGCTGACT 
TTGAATGTCA TTGAGAATGA ACAGATGGAA AATACCCAGA GGGCAGAGCA TGAGGAGGGG 
AAGGTCAAGG ATCTATTGGC TGAGTCACGG TTGAGATACA AAGACTACAT CCAAATCCTT 
AGCAGCCCAA ACTTCAGCCT CGACCAGTAC TGCGAACAGA TGTGGCACAG GGAGAAGCGG 
AGACAGAGAA ACAAGGGGGG CCCAAAGTGG AAGCACATGC AGGAAATGAA GAAGAAACGA 
AATCGAAGAC ATCACAGAGA CCTGGATGAG CTCCCTAGAG CTGTAGCCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689967.2
CDS	41..2374
Translation	MNANKDERLKARSQDFHLFPALMMLSMTMLFLPVTGTLKQNIPRLKLTYKDLLLSNSCIPFLGSSEGLDFQTLLLDEERGRLLLGAKDHIFLLSLVDLNKNFKKIYWPAAKERVELCKLAGKDANTECANFIRVLQPYNKTHIYVCGTGAFHPICGYIDLGVYKEDIIFKLDTHNLESGRLKCPFDPQQPFASVMTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGRVCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTYFDELQDIYLLPTRDERNPVVYGVFTTTSSIFKGSAVCVYSMADIRAVFNGPYAHKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISFIKRHSVMYKSVYPVAGGPTFKRINVDYRLTQIVVDHVIAEDGQYDVMFLGTDIGTVLKVVSISKEKWNMEEVVLEELQIFKHSSIILNMELSLKQQQLYIGSRDGLVQLSLHRCDTYGKACADCCLARDPYCAWDGNACSRYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFLECIPKSQQATIKWYIQRSGDEHREELKPDERIIKTEYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEHEEGKVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQMWHREKRRQRNKGGPKWKHMQEMKKKRNRRHHRDLDELPRAVAT

Target ORF information:

RefSeq Version	NM_152754.2
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 3D (SEMA3D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152754.2

ORF Insert Sequence:

ATGAATGCTA ATAAAGATGA AAGACTTAAA GCCAGAAGCC AAGATTTTCA CCTTTTTCCT 
GCTTTGATGA TGCTAAGCAT GACCATGTTG TTTCTTCCAG TCACTGGCAC TTTGAAGCAA 
AATATTCCAA GACTCAAGCT AACCTACAAA GACTTGCTGC TTTCAAATAG CTGTATTCCC 
TTTTTGGGTT CATCAGAAGG ACTGGATTTT CAAACTCTTC TCTTAGATGA GGAAAGAGGC 
AGGCTGCTCT TGGGAGCCAA AGACCACATC TTTCTACTCA GTCTGGTTGA CTTAAACAAA 
AATTTTAAGA AGATTTATTG GCCTGCTGCA AAGGAACGGG TGGAATTATG TAAATTAGCT 
GGGAAAGATG CCAATACAGA ATGTGCAAAT TTCATCAGAG TACTTCAGCC CTATAACAAA 
ACTCACATAT ATGTGTGTGG AACTGGAGCA TTTCATCCAA TATGTGGGTA TATTGATCTT 
GGAGTCTACA AGGAGGATAT TATATTCAAA CTAGACACAC ATAATTTGGA GTCTGGCAGA 
CTGAAATGTC CTTTCGATCC TCAGCAGCCT TTTGCTTCAG TAATGACAGA TGAGTACCTC 
TACTCTGGAA CAGCTTCTGA TTTCCTTGGC AAAGATACTG CATTCACTCG ATCCCTTGGG 
CCTACTCATG ACCACCACTA CATCAGAACT GACATTTCAG AGCACTACTG GCTCAATGGA 
GCAAAATTTA TTGGAACTTT CTTCATACCA GACACCTACA ATCCAGATGA TGATAAAATA 
TATTTCTTCT TTCGTGAATC ATCTCAAGAA GGCAGTACCT CCGATAAAAC CATCCTTTCT 
CGAGTTGGAA GAGTTTGTAA GAATGATGTA GGAGGACAAC GCAGCCTGAT AAACAAGTGG 
ACGACTTTTC TTAAGGCCAG ACTGATTTGC TCAATTCCTG GAAGTGATGG GGCAGATACT 
TACTTTGATG AGCTTCAAGA TATTTATTTA CTCCCCACAA GAGATGAAAG AAATCCTGTA 
GTATATGGAG TCTTTACTAC AACCAGCTCC ATCTTCAAAG GCTCTGCTGT TTGTGTGTAT 
AGCATGGCTG ACATCAGAGC AGTTTTTAAT GGTCCATATG CTCATAAGGA AAGTGCAGAC 
CATCGTTGGG TGCAGTATGA TGGGAGAATT CCTTATCCAC GGCCTGGTAC ATGTCCAAGC 
AAAACCTATG ACCCACTGAT TAAGTCCACC CGAGATTTTC CAGATGATGT CATCAGTTTC 
ATAAAGCGGC ACTCTGTGAT GTATAAGTCC GTATACCCAG TTGCAGGAGG ACCAACGTTC 
AAGAGAATCA ATGTGGATTA CAGACTGACA CAGATAGTGG TGGATCATGT CATTGCAGAA 
GATGGCCAGT ACGATGTAAT GTTTCTTGGA ACAGACATTG GAACTGTCCT CAAAGTTGTC 
AGCATTTCAA AGGAAAAGTG GAATATGGAA GAGGTAGTGC TGGAGGAGTT GCAGATATTC 
AAGCACTCAT CAATCATCTT GAACATGGAA TTGTCTCTGA AGCAGCAACA ATTGTACATT 
GGTTCCCGAG ATGGATTGGT TCAGCTCTCC TTGCACAGAT GCGACACTTA TGGGAAAGCT 
TGCGCAGACT GTTGTCTTGC CAGAGACCCC TACTGTGCCT GGGATGGAAA TGCATGCTCT 
CGATATGCTC CTACTTCTAA AAGGAGAGCT AGACGCCAAG ATGTAAAATA TGGCGACCCA 
ATCACCCAGT GCTGGGACAT CGAAGACAGC ATTAGTCATG AAACTGCTGA TGAAAAGGTG 
ATTTTTGGCA TTGAATTTAA CTCAACCTTT CTGGAATGTA TACCTAAATC CCAACAAGCA 
ACTATTAAAT GGTATATCCA GAGGTCAGGG GATGAGCATC GAGAGGAGTT GAAGCCCGAT 
GAAAGAATCA TCAAAACGGA ATATGGGCTA CTGATTCGAA GTTTGCAGAA GAAGGATTCT 
GGGATGTATT ACTGCAAAGC CCAGGAGCAC ACTTTCATCC ACACCATAGT GAAGCTGACT 
TTGAATGTCA TTGAGAATGA ACAGATGGAA AATACCCAGA GGGCAGAGCA TGAGGAGGGG 
AAGGTCAAGG ATCTATTGGC TGAGTCACGG TTGAGATACA AAGACTACAT CCAAATCCTT 
AGCAGCCCAA ACTTCAGCCT CGACCAGTAC TGCGAACAGA TGTGGCACAG GGAGAAGCGG 
AGACAGAGAA ACAAGGGGGG CCCAAAGTGG AAGCACATGC AGGAAATGAA GAAGAAACGA 
AATCGAAGAC ATCACAGAGA CCTGGATGAG CTCCCTAGAG CTGTAGCCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA3D ( XM_017011873.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867362.1 Homo sapiens semaphorin 3D (SEMA3D), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu06915
Clone ID Related Accession (Same CDS sequence)	NM_152754.2 , XM_017011873.1 , XM_011515960.1
Accession Version	XM_017011873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	semaphorin-3D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCTA ATAAAGATGA AAGACTTAAA GCCAGAAGCC AAGATTTTCA CCTTTTTCCT 
GCTTTGATGA TGCTAAGCAT GACCATGTTG TTTCTTCCAG TCACTGGCAC TTTGAAGCAA 
AATATTCCAA GACTCAAGCT AACCTACAAA GACTTGCTGC TTTCAAATAG CTGTATTCCC 
TTTTTGGGTT CATCAGAAGG ACTGGATTTT CAAACTCTTC TCTTAGATGA GGAAAGAGGC 
AGGCTGCTCT TGGGAGCCAA AGACCACATC TTTCTACTCA GTCTGGTTGA CTTAAACAAA 
AATTTTAAGA AGATTTATTG GCCTGCTGCA AAGGAACGGG TGGAATTATG TAAATTAGCT 
GGGAAAGATG CCAATACAGA ATGTGCAAAT TTCATCAGAG TACTTCAGCC CTATAACAAA 
ACTCACATAT ATGTGTGTGG AACTGGAGCA TTTCATCCAA TATGTGGGTA TATTGATCTT 
GGAGTCTACA AGGAGGATAT TATATTCAAA CTAGACACAC ATAATTTGGA GTCTGGCAGA 
CTGAAATGTC CTTTCGATCC TCAGCAGCCT TTTGCTTCAG TAATGACAGA TGAGTACCTC 
TACTCTGGAA CAGCTTCTGA TTTCCTTGGC AAAGATACTG CATTCACTCG ATCCCTTGGG 
CCTACTCATG ACCACCACTA CATCAGAACT GACATTTCAG AGCACTACTG GCTCAATGGA 
GCAAAATTTA TTGGAACTTT CTTCATACCA GACACCTACA ATCCAGATGA TGATAAAATA 
TATTTCTTCT TTCGTGAATC ATCTCAAGAA GGCAGTACCT CCGATAAAAC CATCCTTTCT 
CGAGTTGGAA GAGTTTGTAA GAATGATGTA GGAGGACAAC GCAGCCTGAT AAACAAGTGG 
ACGACTTTTC TTAAGGCCAG ACTGATTTGC TCAATTCCTG GAAGTGATGG GGCAGATACT 
TACTTTGATG AGCTTCAAGA TATTTATTTA CTCCCCACAA GAGATGAAAG AAATCCTGTA 
GTATATGGAG TCTTTACTAC AACCAGCTCC ATCTTCAAAG GCTCTGCTGT TTGTGTGTAT 
AGCATGGCTG ACATCAGAGC AGTTTTTAAT GGTCCATATG CTCATAAGGA AAGTGCAGAC 
CATCGTTGGG TGCAGTATGA TGGGAGAATT CCTTATCCAC GGCCTGGTAC ATGTCCAAGC 
AAAACCTATG ACCCACTGAT TAAGTCCACC CGAGATTTTC CAGATGATGT CATCAGTTTC 
ATAAAGCGGC ACTCTGTGAT GTATAAGTCC GTATACCCAG TTGCAGGAGG ACCAACGTTC 
AAGAGAATCA ATGTGGATTA CAGACTGACA CAGATAGTGG TGGATCATGT CATTGCAGAA 
GATGGCCAGT ACGATGTAAT GTTTCTTGGA ACAGACATTG GAACTGTCCT CAAAGTTGTC 
AGCATTTCAA AGGAAAAGTG GAATATGGAA GAGGTAGTGC TGGAGGAGTT GCAGATATTC 
AAGCACTCAT CAATCATCTT GAACATGGAA TTGTCTCTGA AGCAGCAACA ATTGTACATT 
GGTTCCCGAG ATGGATTGGT TCAGCTCTCC TTGCACAGAT GCGACACTTA TGGGAAAGCT 
TGCGCAGACT GTTGTCTTGC CAGAGACCCC TACTGTGCCT GGGATGGAAA TGCATGCTCT 
CGATATGCTC CTACTTCTAA AAGGAGAGCT AGACGCCAAG ATGTAAAATA TGGCGACCCA 
ATCACCCAGT GCTGGGACAT CGAAGACAGC ATTAGTCATG AAACTGCTGA TGAAAAGGTG 
ATTTTTGGCA TTGAATTTAA CTCAACCTTT CTGGAATGTA TACCTAAATC CCAACAAGCA 
ACTATTAAAT GGTATATCCA GAGGTCAGGG GATGAGCATC GAGAGGAGTT GAAGCCCGAT 
GAAAGAATCA TCAAAACGGA ATATGGGCTA CTGATTCGAA GTTTGCAGAA GAAGGATTCT 
GGGATGTATT ACTGCAAAGC CCAGGAGCAC ACTTTCATCC ACACCATAGT GAAGCTGACT 
TTGAATGTCA TTGAGAATGA ACAGATGGAA AATACCCAGA GGGCAGAGCA TGAGGAGGGG 
AAGGTCAAGG ATCTATTGGC TGAGTCACGG TTGAGATACA AAGACTACAT CCAAATCCTT 
AGCAGCCCAA ACTTCAGCCT CGACCAGTAC TGCGAACAGA TGTGGCACAG GGAGAAGCGG 
AGACAGAGAA ACAAGGGGGG CCCAAAGTGG AAGCACATGC AGGAAATGAA GAAGAAACGA 
AATCGAAGAC ATCACAGAGA CCTGGATGAG CTCCCTAGAG CTGTAGCCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867362.1
CDS	540..2873
Translation	MNANKDERLKARSQDFHLFPALMMLSMTMLFLPVTGTLKQNIPRLKLTYKDLLLSNSCIPFLGSSEGLDFQTLLLDEERGRLLLGAKDHIFLLSLVDLNKNFKKIYWPAAKERVELCKLAGKDANTECANFIRVLQPYNKTHIYVCGTGAFHPICGYIDLGVYKEDIIFKLDTHNLESGRLKCPFDPQQPFASVMTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGRVCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTYFDELQDIYLLPTRDERNPVVYGVFTTTSSIFKGSAVCVYSMADIRAVFNGPYAHKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISFIKRHSVMYKSVYPVAGGPTFKRINVDYRLTQIVVDHVIAEDGQYDVMFLGTDIGTVLKVVSISKEKWNMEEVVLEELQIFKHSSIILNMELSLKQQQLYIGSRDGLVQLSLHRCDTYGKACADCCLARDPYCAWDGNACSRYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFLECIPKSQQATIKWYIQRSGDEHREELKPDERIIKTEYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEHEEGKVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQMWHREKRRQRNKGGPKWKHMQEMKKKRNRRHHRDLDELPRAVAT

Target ORF information:

RefSeq Version	XM_017011873.1
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 3D (SEMA3D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011873.1

ORF Insert Sequence:

ATGAATGCTA ATAAAGATGA AAGACTTAAA GCCAGAAGCC AAGATTTTCA CCTTTTTCCT 
GCTTTGATGA TGCTAAGCAT GACCATGTTG TTTCTTCCAG TCACTGGCAC TTTGAAGCAA 
AATATTCCAA GACTCAAGCT AACCTACAAA GACTTGCTGC TTTCAAATAG CTGTATTCCC 
TTTTTGGGTT CATCAGAAGG ACTGGATTTT CAAACTCTTC TCTTAGATGA GGAAAGAGGC 
AGGCTGCTCT TGGGAGCCAA AGACCACATC TTTCTACTCA GTCTGGTTGA CTTAAACAAA 
AATTTTAAGA AGATTTATTG GCCTGCTGCA AAGGAACGGG TGGAATTATG TAAATTAGCT 
GGGAAAGATG CCAATACAGA ATGTGCAAAT TTCATCAGAG TACTTCAGCC CTATAACAAA 
ACTCACATAT ATGTGTGTGG AACTGGAGCA TTTCATCCAA TATGTGGGTA TATTGATCTT 
GGAGTCTACA AGGAGGATAT TATATTCAAA CTAGACACAC ATAATTTGGA GTCTGGCAGA 
CTGAAATGTC CTTTCGATCC TCAGCAGCCT TTTGCTTCAG TAATGACAGA TGAGTACCTC 
TACTCTGGAA CAGCTTCTGA TTTCCTTGGC AAAGATACTG CATTCACTCG ATCCCTTGGG 
CCTACTCATG ACCACCACTA CATCAGAACT GACATTTCAG AGCACTACTG GCTCAATGGA 
GCAAAATTTA TTGGAACTTT CTTCATACCA GACACCTACA ATCCAGATGA TGATAAAATA 
TATTTCTTCT TTCGTGAATC ATCTCAAGAA GGCAGTACCT CCGATAAAAC CATCCTTTCT 
CGAGTTGGAA GAGTTTGTAA GAATGATGTA GGAGGACAAC GCAGCCTGAT AAACAAGTGG 
ACGACTTTTC TTAAGGCCAG ACTGATTTGC TCAATTCCTG GAAGTGATGG GGCAGATACT 
TACTTTGATG AGCTTCAAGA TATTTATTTA CTCCCCACAA GAGATGAAAG AAATCCTGTA 
GTATATGGAG TCTTTACTAC AACCAGCTCC ATCTTCAAAG GCTCTGCTGT TTGTGTGTAT 
AGCATGGCTG ACATCAGAGC AGTTTTTAAT GGTCCATATG CTCATAAGGA AAGTGCAGAC 
CATCGTTGGG TGCAGTATGA TGGGAGAATT CCTTATCCAC GGCCTGGTAC ATGTCCAAGC 
AAAACCTATG ACCCACTGAT TAAGTCCACC CGAGATTTTC CAGATGATGT CATCAGTTTC 
ATAAAGCGGC ACTCTGTGAT GTATAAGTCC GTATACCCAG TTGCAGGAGG ACCAACGTTC 
AAGAGAATCA ATGTGGATTA CAGACTGACA CAGATAGTGG TGGATCATGT CATTGCAGAA 
GATGGCCAGT ACGATGTAAT GTTTCTTGGA ACAGACATTG GAACTGTCCT CAAAGTTGTC 
AGCATTTCAA AGGAAAAGTG GAATATGGAA GAGGTAGTGC TGGAGGAGTT GCAGATATTC 
AAGCACTCAT CAATCATCTT GAACATGGAA TTGTCTCTGA AGCAGCAACA ATTGTACATT 
GGTTCCCGAG ATGGATTGGT TCAGCTCTCC TTGCACAGAT GCGACACTTA TGGGAAAGCT 
TGCGCAGACT GTTGTCTTGC CAGAGACCCC TACTGTGCCT GGGATGGAAA TGCATGCTCT 
CGATATGCTC CTACTTCTAA AAGGAGAGCT AGACGCCAAG ATGTAAAATA TGGCGACCCA 
ATCACCCAGT GCTGGGACAT CGAAGACAGC ATTAGTCATG AAACTGCTGA TGAAAAGGTG 
ATTTTTGGCA TTGAATTTAA CTCAACCTTT CTGGAATGTA TACCTAAATC CCAACAAGCA 
ACTATTAAAT GGTATATCCA GAGGTCAGGG GATGAGCATC GAGAGGAGTT GAAGCCCGAT 
GAAAGAATCA TCAAAACGGA ATATGGGCTA CTGATTCGAA GTTTGCAGAA GAAGGATTCT 
GGGATGTATT ACTGCAAAGC CCAGGAGCAC ACTTTCATCC ACACCATAGT GAAGCTGACT 
TTGAATGTCA TTGAGAATGA ACAGATGGAA AATACCCAGA GGGCAGAGCA TGAGGAGGGG 
AAGGTCAAGG ATCTATTGGC TGAGTCACGG TTGAGATACA AAGACTACAT CCAAATCCTT 
AGCAGCCCAA ACTTCAGCCT CGACCAGTAC TGCGAACAGA TGTGGCACAG GGAGAAGCGG 
AGACAGAGAA ACAAGGGGGG CCCAAAGTGG AAGCACATGC AGGAAATGAA GAAGAAACGA 
AATCGAAGAC ATCACAGAGA CCTGGATGAG CTCCCTAGAG CTGTAGCCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA3D ( XM_011515960.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514262.1 Homo sapiens semaphorin 3D (SEMA3D), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu06915
Clone ID Related Accession (Same CDS sequence)	NM_152754.2 , XM_017011873.1 , XM_011515960.1
Accession Version	XM_011515960.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	semaphorin-3D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCTA ATAAAGATGA AAGACTTAAA GCCAGAAGCC AAGATTTTCA CCTTTTTCCT 
GCTTTGATGA TGCTAAGCAT GACCATGTTG TTTCTTCCAG TCACTGGCAC TTTGAAGCAA 
AATATTCCAA GACTCAAGCT AACCTACAAA GACTTGCTGC TTTCAAATAG CTGTATTCCC 
TTTTTGGGTT CATCAGAAGG ACTGGATTTT CAAACTCTTC TCTTAGATGA GGAAAGAGGC 
AGGCTGCTCT TGGGAGCCAA AGACCACATC TTTCTACTCA GTCTGGTTGA CTTAAACAAA 
AATTTTAAGA AGATTTATTG GCCTGCTGCA AAGGAACGGG TGGAATTATG TAAATTAGCT 
GGGAAAGATG CCAATACAGA ATGTGCAAAT TTCATCAGAG TACTTCAGCC CTATAACAAA 
ACTCACATAT ATGTGTGTGG AACTGGAGCA TTTCATCCAA TATGTGGGTA TATTGATCTT 
GGAGTCTACA AGGAGGATAT TATATTCAAA CTAGACACAC ATAATTTGGA GTCTGGCAGA 
CTGAAATGTC CTTTCGATCC TCAGCAGCCT TTTGCTTCAG TAATGACAGA TGAGTACCTC 
TACTCTGGAA CAGCTTCTGA TTTCCTTGGC AAAGATACTG CATTCACTCG ATCCCTTGGG 
CCTACTCATG ACCACCACTA CATCAGAACT GACATTTCAG AGCACTACTG GCTCAATGGA 
GCAAAATTTA TTGGAACTTT CTTCATACCA GACACCTACA ATCCAGATGA TGATAAAATA 
TATTTCTTCT TTCGTGAATC ATCTCAAGAA GGCAGTACCT CCGATAAAAC CATCCTTTCT 
CGAGTTGGAA GAGTTTGTAA GAATGATGTA GGAGGACAAC GCAGCCTGAT AAACAAGTGG 
ACGACTTTTC TTAAGGCCAG ACTGATTTGC TCAATTCCTG GAAGTGATGG GGCAGATACT 
TACTTTGATG AGCTTCAAGA TATTTATTTA CTCCCCACAA GAGATGAAAG AAATCCTGTA 
GTATATGGAG TCTTTACTAC AACCAGCTCC ATCTTCAAAG GCTCTGCTGT TTGTGTGTAT 
AGCATGGCTG ACATCAGAGC AGTTTTTAAT GGTCCATATG CTCATAAGGA AAGTGCAGAC 
CATCGTTGGG TGCAGTATGA TGGGAGAATT CCTTATCCAC GGCCTGGTAC ATGTCCAAGC 
AAAACCTATG ACCCACTGAT TAAGTCCACC CGAGATTTTC CAGATGATGT CATCAGTTTC 
ATAAAGCGGC ACTCTGTGAT GTATAAGTCC GTATACCCAG TTGCAGGAGG ACCAACGTTC 
AAGAGAATCA ATGTGGATTA CAGACTGACA CAGATAGTGG TGGATCATGT CATTGCAGAA 
GATGGCCAGT ACGATGTAAT GTTTCTTGGA ACAGACATTG GAACTGTCCT CAAAGTTGTC 
AGCATTTCAA AGGAAAAGTG GAATATGGAA GAGGTAGTGC TGGAGGAGTT GCAGATATTC 
AAGCACTCAT CAATCATCTT GAACATGGAA TTGTCTCTGA AGCAGCAACA ATTGTACATT 
GGTTCCCGAG ATGGATTGGT TCAGCTCTCC TTGCACAGAT GCGACACTTA TGGGAAAGCT 
TGCGCAGACT GTTGTCTTGC CAGAGACCCC TACTGTGCCT GGGATGGAAA TGCATGCTCT 
CGATATGCTC CTACTTCTAA AAGGAGAGCT AGACGCCAAG ATGTAAAATA TGGCGACCCA 
ATCACCCAGT GCTGGGACAT CGAAGACAGC ATTAGTCATG AAACTGCTGA TGAAAAGGTG 
ATTTTTGGCA TTGAATTTAA CTCAACCTTT CTGGAATGTA TACCTAAATC CCAACAAGCA 
ACTATTAAAT GGTATATCCA GAGGTCAGGG GATGAGCATC GAGAGGAGTT GAAGCCCGAT 
GAAAGAATCA TCAAAACGGA ATATGGGCTA CTGATTCGAA GTTTGCAGAA GAAGGATTCT 
GGGATGTATT ACTGCAAAGC CCAGGAGCAC ACTTTCATCC ACACCATAGT GAAGCTGACT 
TTGAATGTCA TTGAGAATGA ACAGATGGAA AATACCCAGA GGGCAGAGCA TGAGGAGGGG 
AAGGTCAAGG ATCTATTGGC TGAGTCACGG TTGAGATACA AAGACTACAT CCAAATCCTT 
AGCAGCCCAA ACTTCAGCCT CGACCAGTAC TGCGAACAGA TGTGGCACAG GGAGAAGCGG 
AGACAGAGAA ACAAGGGGGG CCCAAAGTGG AAGCACATGC AGGAAATGAA GAAGAAACGA 
AATCGAAGAC ATCACAGAGA CCTGGATGAG CTCCCTAGAG CTGTAGCCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514262.1
CDS	351..2684
Translation	MNANKDERLKARSQDFHLFPALMMLSMTMLFLPVTGTLKQNIPRLKLTYKDLLLSNSCIPFLGSSEGLDFQTLLLDEERGRLLLGAKDHIFLLSLVDLNKNFKKIYWPAAKERVELCKLAGKDANTECANFIRVLQPYNKTHIYVCGTGAFHPICGYIDLGVYKEDIIFKLDTHNLESGRLKCPFDPQQPFASVMTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGRVCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTYFDELQDIYLLPTRDERNPVVYGVFTTTSSIFKGSAVCVYSMADIRAVFNGPYAHKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISFIKRHSVMYKSVYPVAGGPTFKRINVDYRLTQIVVDHVIAEDGQYDVMFLGTDIGTVLKVVSISKEKWNMEEVVLEELQIFKHSSIILNMELSLKQQQLYIGSRDGLVQLSLHRCDTYGKACADCCLARDPYCAWDGNACSRYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFLECIPKSQQATIKWYIQRSGDEHREELKPDERIIKTEYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEHEEGKVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQMWHREKRRQRNKGGPKWKHMQEMKKKRNRRHHRDLDELPRAVAT

Target ORF information:

RefSeq Version	XM_011515960.1
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 3D (SEMA3D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515960.1

ORF Insert Sequence:

ATGAATGCTA ATAAAGATGA AAGACTTAAA GCCAGAAGCC AAGATTTTCA CCTTTTTCCT 
GCTTTGATGA TGCTAAGCAT GACCATGTTG TTTCTTCCAG TCACTGGCAC TTTGAAGCAA 
AATATTCCAA GACTCAAGCT AACCTACAAA GACTTGCTGC TTTCAAATAG CTGTATTCCC 
TTTTTGGGTT CATCAGAAGG ACTGGATTTT CAAACTCTTC TCTTAGATGA GGAAAGAGGC 
AGGCTGCTCT TGGGAGCCAA AGACCACATC TTTCTACTCA GTCTGGTTGA CTTAAACAAA 
AATTTTAAGA AGATTTATTG GCCTGCTGCA AAGGAACGGG TGGAATTATG TAAATTAGCT 
GGGAAAGATG CCAATACAGA ATGTGCAAAT TTCATCAGAG TACTTCAGCC CTATAACAAA 
ACTCACATAT ATGTGTGTGG AACTGGAGCA TTTCATCCAA TATGTGGGTA TATTGATCTT 
GGAGTCTACA AGGAGGATAT TATATTCAAA CTAGACACAC ATAATTTGGA GTCTGGCAGA 
CTGAAATGTC CTTTCGATCC TCAGCAGCCT TTTGCTTCAG TAATGACAGA TGAGTACCTC 
TACTCTGGAA CAGCTTCTGA TTTCCTTGGC AAAGATACTG CATTCACTCG ATCCCTTGGG 
CCTACTCATG ACCACCACTA CATCAGAACT GACATTTCAG AGCACTACTG GCTCAATGGA 
GCAAAATTTA TTGGAACTTT CTTCATACCA GACACCTACA ATCCAGATGA TGATAAAATA 
TATTTCTTCT TTCGTGAATC ATCTCAAGAA GGCAGTACCT CCGATAAAAC CATCCTTTCT 
CGAGTTGGAA GAGTTTGTAA GAATGATGTA GGAGGACAAC GCAGCCTGAT AAACAAGTGG 
ACGACTTTTC TTAAGGCCAG ACTGATTTGC TCAATTCCTG GAAGTGATGG GGCAGATACT 
TACTTTGATG AGCTTCAAGA TATTTATTTA CTCCCCACAA GAGATGAAAG AAATCCTGTA 
GTATATGGAG TCTTTACTAC AACCAGCTCC ATCTTCAAAG GCTCTGCTGT TTGTGTGTAT 
AGCATGGCTG ACATCAGAGC AGTTTTTAAT GGTCCATATG CTCATAAGGA AAGTGCAGAC 
CATCGTTGGG TGCAGTATGA TGGGAGAATT CCTTATCCAC GGCCTGGTAC ATGTCCAAGC 
AAAACCTATG ACCCACTGAT TAAGTCCACC CGAGATTTTC CAGATGATGT CATCAGTTTC 
ATAAAGCGGC ACTCTGTGAT GTATAAGTCC GTATACCCAG TTGCAGGAGG ACCAACGTTC 
AAGAGAATCA ATGTGGATTA CAGACTGACA CAGATAGTGG TGGATCATGT CATTGCAGAA 
GATGGCCAGT ACGATGTAAT GTTTCTTGGA ACAGACATTG GAACTGTCCT CAAAGTTGTC 
AGCATTTCAA AGGAAAAGTG GAATATGGAA GAGGTAGTGC TGGAGGAGTT GCAGATATTC 
AAGCACTCAT CAATCATCTT GAACATGGAA TTGTCTCTGA AGCAGCAACA ATTGTACATT 
GGTTCCCGAG ATGGATTGGT TCAGCTCTCC TTGCACAGAT GCGACACTTA TGGGAAAGCT 
TGCGCAGACT GTTGTCTTGC CAGAGACCCC TACTGTGCCT GGGATGGAAA TGCATGCTCT 
CGATATGCTC CTACTTCTAA AAGGAGAGCT AGACGCCAAG ATGTAAAATA TGGCGACCCA 
ATCACCCAGT GCTGGGACAT CGAAGACAGC ATTAGTCATG AAACTGCTGA TGAAAAGGTG 
ATTTTTGGCA TTGAATTTAA CTCAACCTTT CTGGAATGTA TACCTAAATC CCAACAAGCA 
ACTATTAAAT GGTATATCCA GAGGTCAGGG GATGAGCATC GAGAGGAGTT GAAGCCCGAT 
GAAAGAATCA TCAAAACGGA ATATGGGCTA CTGATTCGAA GTTTGCAGAA GAAGGATTCT 
GGGATGTATT ACTGCAAAGC CCAGGAGCAC ACTTTCATCC ACACCATAGT GAAGCTGACT 
TTGAATGTCA TTGAGAATGA ACAGATGGAA AATACCCAGA GGGCAGAGCA TGAGGAGGGG 
AAGGTCAAGG ATCTATTGGC TGAGTCACGG TTGAGATACA AAGACTACAT CCAAATCCTT 
AGCAGCCCAA ACTTCAGCCT CGACCAGTAC TGCGAACAGA TGTGGCACAG GGAGAAGCGG 
AGACAGAGAA ACAAGGGGGG CCCAAAGTGG AAGCACATGC AGGAAATGAA GAAGAAACGA 
AATCGAAGAC ATCACAGAGA CCTGGATGAG CTCCCTAGAG CTGTAGCCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA3D ( XM_011515961.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514263.1 Homo sapiens semaphorin 3D (SEMA3D), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu78152
Clone ID Related Accession (Same CDS sequence)	XM_011515961.2
Accession Version	XM_011515961.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	semaphorin-3D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGATG AGTACCTCTA CTCTGGAACA GCTTCTGATT TCCTTGGCAA AGATACTGCA 
TTCACTCGAT CCCTTGGGCC TACTCATGAC CACCACTACA TCAGAACTGA CATTTCAGAG 
CACTACTGGC TCAATGGAGC AAAATTTATT GGAACTTTCT TCATACCAGA CACCTACAAT 
CCAGATGATG ATAAAATATA TTTCTTCTTT CGTGAATCAT CTCAAGAAGG CAGTACCTCC 
GATAAAACCA TCCTTTCTCG AGTTGGAAGA GTTTGTAAGA ATGATGTAGG AGGACAACGC 
AGCCTGATAA ACAAGTGGAC GACTTTTCTT AAGGCCAGAC TGATTTGCTC AATTCCTGGA 
AGTGATGGGG CAGATACTTA CTTTGATGAG CTTCAAGATA TTTATTTACT CCCCACAAGA 
GATGAAAGAA ATCCTGTAGT ATATGGAGTC TTTACTACAA CCAGCTCCAT CTTCAAAGGC 
TCTGCTGTTT GTGTGTATAG CATGGCTGAC ATCAGAGCAG TTTTTAATGG TCCATATGCT 
CATAAGGAAA GTGCAGACCA TCGTTGGGTG CAGTATGATG GGAGAATTCC TTATCCACGG 
CCTGGTACAT GTCCAAGCAA AACCTATGAC CCACTGATTA AGTCCACCCG AGATTTTCCA 
GATGATGTCA TCAGTTTCAT AAAGCGGCAC TCTGTGATGT ATAAGTCCGT ATACCCAGTT 
GCAGGAGGAC CAACGTTCAA GAGAATCAAT GTGGATTACA GACTGACACA GATAGTGGTG 
GATCATGTCA TTGCAGAAGA TGGCCAGTAC GATGTAATGT TTCTTGGAAC AGACATTGGA 
ACTGTCCTCA AAGTTGTCAG CATTTCAAAG GAAAAGTGGA ATATGGAAGA GGTAGTGCTG 
GAGGAGTTGC AGATATTCAA GCACTCATCA ATCATCTTGA ACATGGAATT GTCTCTGAAG 
CAGCAACAAT TGTACATTGG TTCCCGAGAT GGATTGGTTC AGCTCTCCTT GCACAGATGC 
GACACTTATG GGAAAGCTTG CGCAGACTGT TGTCTTGCCA GAGACCCCTA CTGTGCCTGG 
GATGGAAATG CATGCTCTCG ATATGCTCCT ACTTCTAAAA GGAGAGCTAG ACGCCAAGAT 
GTAAAATATG GCGACCCAAT CACCCAGTGC TGGGACATCG AAGACAGCAT TAGTCATGAA 
ACTGCTGATG AAAAGGTGAT TTTTGGCATT GAATTTAACT CAACCTTTCT GGAATGTATA 
CCTAAATCCC AACAAGCAAC TATTAAATGG TATATCCAGA GGTCAGGGGA TGAGCATCGA 
GAGGAGTTGA AGCCCGATGA AAGAATCATC AAAACGGAAT ATGGGCTACT GATTCGAAGT 
TTGCAGAAGA AGGATTCTGG GATGTATTAC TGCAAAGCCC AGGAGCACAC TTTCATCCAC 
ACCATAGTGA AGCTGACTTT GAATGTCATT GAGAATGAAC AGATGGAAAA TACCCAGAGG 
GCAGAGCATG AGGAGGGGAA GGTCAAGGAT CTATTGGCTG AGTCACGGTT GAGATACAAA 
GACTACATCC AAATCCTTAG CAGCCCAAAC TTCAGCCTCG ACCAGTACTG CGAACAGATG 
TGGCACAGGG AGAAGCGGAG ACAGAGAAAC AAGGGGGGCC CAAAGTGGAA GCACATGCAG 
GAAATGAAGA AGAAACGAAA TCGAAGACAT CACAGAGACC TGGATGAGCT CCCTAGAGCT 
GTAGCCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514263.1
CDS	458..2209
Translation	MTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGRVCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTYFDELQDIYLLPTRDERNPVVYGVFTTTSSIFKGSAVCVYSMADIRAVFNGPYAHKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISFIKRHSVMYKSVYPVAGGPTFKRINVDYRLTQIVVDHVIAEDGQYDVMFLGTDIGTVLKVVSISKEKWNMEEVVLEELQIFKHSSIILNMELSLKQQQLYIGSRDGLVQLSLHRCDTYGKACADCCLARDPYCAWDGNACSRYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFLECIPKSQQATIKWYIQRSGDEHREELKPDERIIKTEYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEHEEGKVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQMWHREKRRQRNKGGPKWKHMQEMKKKRNRRHHRDLDELPRAVAT

Target ORF information:

RefSeq Version	XM_011515961.2
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 3D (SEMA3D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515961.2

ORF Insert Sequence:

ATGACAGATG AGTACCTCTA CTCTGGAACA GCTTCTGATT TCCTTGGCAA AGATACTGCA 
TTCACTCGAT CCCTTGGGCC TACTCATGAC CACCACTACA TCAGAACTGA CATTTCAGAG 
CACTACTGGC TCAATGGAGC AAAATTTATT GGAACTTTCT TCATACCAGA CACCTACAAT 
CCAGATGATG ATAAAATATA TTTCTTCTTT CGTGAATCAT CTCAAGAAGG CAGTACCTCC 
GATAAAACCA TCCTTTCTCG AGTTGGAAGA GTTTGTAAGA ATGATGTAGG AGGACAACGC 
AGCCTGATAA ACAAGTGGAC GACTTTTCTT AAGGCCAGAC TGATTTGCTC AATTCCTGGA 
AGTGATGGGG CAGATACTTA CTTTGATGAG CTTCAAGATA TTTATTTACT CCCCACAAGA 
GATGAAAGAA ATCCTGTAGT ATATGGAGTC TTTACTACAA CCAGCTCCAT CTTCAAAGGC 
TCTGCTGTTT GTGTGTATAG CATGGCTGAC ATCAGAGCAG TTTTTAATGG TCCATATGCT 
CATAAGGAAA GTGCAGACCA TCGTTGGGTG CAGTATGATG GGAGAATTCC TTATCCACGG 
CCTGGTACAT GTCCAAGCAA AACCTATGAC CCACTGATTA AGTCCACCCG AGATTTTCCA 
GATGATGTCA TCAGTTTCAT AAAGCGGCAC TCTGTGATGT ATAAGTCCGT ATACCCAGTT 
GCAGGAGGAC CAACGTTCAA GAGAATCAAT GTGGATTACA GACTGACACA GATAGTGGTG 
GATCATGTCA TTGCAGAAGA TGGCCAGTAC GATGTAATGT TTCTTGGAAC AGACATTGGA 
ACTGTCCTCA AAGTTGTCAG CATTTCAAAG GAAAAGTGGA ATATGGAAGA GGTAGTGCTG 
GAGGAGTTGC AGATATTCAA GCACTCATCA ATCATCTTGA ACATGGAATT GTCTCTGAAG 
CAGCAACAAT TGTACATTGG TTCCCGAGAT GGATTGGTTC AGCTCTCCTT GCACAGATGC 
GACACTTATG GGAAAGCTTG CGCAGACTGT TGTCTTGCCA GAGACCCCTA CTGTGCCTGG 
GATGGAAATG CATGCTCTCG ATATGCTCCT ACTTCTAAAA GGAGAGCTAG ACGCCAAGAT 
GTAAAATATG GCGACCCAAT CACCCAGTGC TGGGACATCG AAGACAGCAT TAGTCATGAA 
ACTGCTGATG AAAAGGTGAT TTTTGGCATT GAATTTAACT CAACCTTTCT GGAATGTATA 
CCTAAATCCC AACAAGCAAC TATTAAATGG TATATCCAGA GGTCAGGGGA TGAGCATCGA 
GAGGAGTTGA AGCCCGATGA AAGAATCATC AAAACGGAAT ATGGGCTACT GATTCGAAGT 
TTGCAGAAGA AGGATTCTGG GATGTATTAC TGCAAAGCCC AGGAGCACAC TTTCATCCAC 
ACCATAGTGA AGCTGACTTT GAATGTCATT GAGAATGAAC AGATGGAAAA TACCCAGAGG 
GCAGAGCATG AGGAGGGGAA GGTCAAGGAT CTATTGGCTG AGTCACGGTT GAGATACAAA 
GACTACATCC AAATCCTTAG CAGCCCAAAC TTCAGCCTCG ACCAGTACTG CGAACAGATG 
TGGCACAGGG AGAAGCGGAG ACAGAGAAAC AAGGGGGGCC CAAAGTGGAA GCACATGCAG 
GAAATGAAGA AGAAACGAAA TCGAAGACAT CACAGAGACC TGGATGAGCT CCCTAGAGCT 
GTAGCCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Promoter-level transcriptome in primary lesions of endometrial cancer identified biomarkers associated with lymph node metastasis.
Scientific reports7(1)14160(2017 Oct)
Yoshida E,Terao Y,Hayashi N,Mogushi K,Arakawa A,Tanaka Y,Ito Y,Ohmiya H,Hayashizaki Y,Takeda S,Itoh M,Kawaji H

Decreased expression of semaphorin 3D is associated with genesis and development in colorectal cancer.
World journal of surgical oncology15(1)67(2017 Mar)
Wang Z,Ding M,Qian N,Song B,Yu J,Tang J,Wang J

Variable expressivity and genetic heterogeneity involving DPT and SEMA3D genes in autosomal dominant familial Meniere's disease.
European journal of human genetics : EJHG25(2)200-207(2017 02)
Mart?n-Sierra C,Gallego-Martinez A,Requena T,Frejo L,Batuecas-Caletr?o A,Lopez-Escamez JA

CTNNA3 and SEMA3D: Promising loci for asthma exacerbation identified through multiple genome-wide association studies.
The Journal of allergy and clinical immunology136(6)1503-1510(2015 Dec)
McGeachie MJ,Wu AC,Tse SM,Clemmer GL,Sordillo J,Himes BE,Lasky-Su J,Chase RP,Martinez FD,Weeke P,Shaffer CM,Xu H,Denny JC,Roden DM,Panettieri RA Jr,Raby BA,Weiss ST,Tantisira KG

Class 3 semaphorins expression and association with innervation and angiogenesis within the degenerate human intervertebral disc.
Oncotarget6(21)18338-54(2015 Jul)
Binch AL,Cole AA,Breakwell LM,Michael AL,Chiverton N,Creemers LB,Cross AK,Le Maitre CL

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SEMA3D cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

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会社情報