Sema3d cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sema3d
Entrez Gene ID	108151
Full Name	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
Synonyms	4631426B19Rik
General protein information	Preferred Names sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D Names semaphorin-3D
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_030254046.1	XP_030109906.1	semaphorin-3D isoform X1
NM_028882.4	NP_083158.3	semaphorin-3D precursor

Pathways from BioSystems

Homologies

	SEMA3D	XP_001107897.1
Bos taurus (cattle)	SEMA3D	NP_001193025.1
Homo sapiens (human)	SEMA3D	NP_689967.2
Canis lupus familiaris (dog)	SEMA3D	XP_533138.2
Rattus norvegicus (Norway rat)	Sema3d	NP_001098103.1
Pan troglodytes (chimpanzee)	SEMA3D	XP_527805.2
Mus musculus (house mouse)	Sema3d	NP_083158.3
Gallus gallus (chicken)	SEMA3D	NP_990704.1
Danio rerio (zebrafish)	sema3d	NP_571123.1
Xenopus tropicalis (tropical clawed frog)	sema3d	XP_002935293.2

Gene Ontology
Bibliography

Related articles in PubMed

Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System.
Memic F, Knoflach V, Morarach K, Sadler R, Laranjeira C, Hjerling-Leffler J, Sundstr?m E, Pachnis V, Marklund U
Gastroenterology(2017 Oct)

Semaphorin signaling facilitates cleft formation in the developing salivary gland.
Chung L, Yang TL, Huang HR, Hsu SM, Cheng HJ, Huang PH
Development (Cambridge, England)134(16)2935-45(2007 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors did not find evidence for genetic interaction between Ret and Sema3d affecting survival, presence of myenteric plexus or intestine transcriptome.
Title: Testing the Ret and Sema3d genetic interaction in mouse enteric nervous system development.

Data indicate that annexin A2 (AnxA2) regulates the secretion of semaphorin 3D (Sema3D) from pancreatic ductal adenocarcinoma (PDA) cells.
Title: Semaphorin 3D autocrine signaling mediates the metastatic role of annexin A2 in pancreatic cancer.

Semaphorin 3d requires neuropilin 1 or PI3K/Akt, whereas semaphorin 3e requires plexin D1 in directing endothelial motility.
Title: Semaphorin 3d and semaphorin 3e direct endothelial motility through distinct molecular signaling pathways.

In the absence of Sema3d, endothelial tubes form in a region that is normally avascular
Title: Semaphorin 3d signaling defects are associated with anomalous pulmonary venous connections.

Two proepicardial markers (Scleraxis and Semaphorin3D) were identified that genetically delineate heretofore uncharacterized proepicardial subcompartments.
Title: Distinct compartments of the proepicardial organ give rise to coronary vascular endothelial cells.

The following Sema3d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema3d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19054	NM_028882.4 Latest version!	Mus musculus sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$454.30 $649.00
OMu19054	XM_030254046.1 Latest version!	Mus musculus sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sema3d ( NM_028882.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083158.3 Mus musculus sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d), mRNA.

Price & Availability↑

CloneID	OMu19054
Clone ID Related Accession (Same CDS sequence)	XM_030254046.1 , NM_028882.4
Accession Version	NM_028882.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Mus musculus(house mouse)
Product	semaphorin-3D precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK052671.1, AK163828.1 and CF746948.1. On Jan 1, 2010 this sequence version replaced NM_028882.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK031704.1, AK028900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387, SAMN00849388 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATGTTA CTAAAGATGA GAACCCAAGA TCCAGAAGTC AAGATCTTCA CCTTTTTCAT 
GCTTGGATGA TGTTAATCAT GACGGTGCTC TTTCTTCCTG TCACTGAAAC GTCTAAACAA 
AATATTCCAA GACTCAAGCT AACCTACAAA GACTTGCTGC TTTCAAACAC CTGTATCCCC 
TTTTTGGGTT CATCAGAAGG ATTGGATTTC CAGACTCTTC TTTTGGATGA GGAGAGGGGC 
ATACTGCTCC TAGGAGCCAA AGACCATGTC TTCCTGCTCA GTCTGGTTGA CTTGAACAAG 
AATTTTAAGA AGATTTATTG GCCTGCTGCA AAAGAACGAG TGGAGCTATG TAAATTAGCT 
GGGAAAGATG CCAATGCAGA ATGTGCAAAT TTCATCCGTG TGCTTCAACC CTATAATAAG 
ACTCACGTTT ACGTGTGTGG AACTGGAGCG TTTCATCCGC TGTGTGGGTA CATTGATCTC 
GGCGCCAACA AGGAGGAACT CATATTTAAA CTAGACACGC ACAACCTGGA GTCTGGCAGA 
CTGAAATGTC CCTTTGATCC TCAGCAGCCT TTTGCTTCAG TAATGACAGA TGAGCACCTC 
TACTCTGGAA CAGCTTCTGA TTTCCTTGGC AAAGACACTG CATTCACAAG GTCTCTGGGG 
CTAATGCAGG ACCACCATTC CATCAGAACT GACATTTCAG AGCACCACTG GCTCAATGGA 
GCAAAATTTA TCGGAACATT CCCCATTCCA GACACCTATA ATCCAGATGA TGATAAAATA 
TATTTCTTCT TTCGAGAATC ATCCCAGGAA GGCAGTACTT CTGACAGAAG CATTCTTTCA 
AGAGTTGGAA GAGTTTGTAA GAATGATGTA GGTGGGCAAC GAAGTCTGAT AAACAAATGG 
ACAACTTTTC TAAAGGCAAG ACTGATTTGC TCGATTCCTG GAAGCGATGG GGCAGATACC 
CATTTTGATG AACTCCAAGA TATTTACTTA CTCCCTACGA GAGATGAAAG AAATCCTGTA 
GTATATGGAG TCTTTACCAC AACCAGCTCC ATCTTCAAAG GCTCTGCTGT CTGTGTGTAC 
AGCATGGCTG ATATCCGAGC AGTCTTTAAT GGTCCCTATG CTCATAAGGA AAGTGCTGAC 
CATCGCTGGG TGCAATATGA TGGAAGGATA CCTTACCCCC GACCTGGAAC GTGTCCAAGC 
AAAACCTATG ACCCACTGAT TAAGTCCACC CGAGACTTTC CAGACGATGT TATCAGTTTC 
ATAAGGCGGC ACCCTGTGAT GTATAAGTCC GTGTACCCAG TGGCAGGAGC ACCGACCTTC 
AAGAGAATCA ACGTGGATTA CAGACTGACG CAGATAGTGG TGGATCACGT GGTCGCTGAA 
GACGGGCAGT ATGATGTCAT GTTTCTCGGA ACAGACATTG GAACAGTCCT GAAAGTTGTG 
AGCATCTCCA AGGAGAAGTG GAATATGGAA GAGGTCGTAC TGGAGGAGCT TCAGGTATTC 
AAGCACCCAA CAGCTATCTT GAACATGGAG TTGTCGCTGA AGCAGCAACA GTTGTACGTT 
GGTTCCTGGG ATGGATTGGT CCAGCTCTCC TTGCACAGAT GCGACACTTA CGGGAAAGCA 
TGTGCAGACT GCTGTCTCGC CAGAGACCCT TACTGTGCCT GGGATGGAAA TGCTTGCTCC 
AGATATGCAC CCACGTCTAA AAGGCGAGCT AGACGCCAGG ATGTAAAATA TGGGGACCCA 
ATCACTCAGT GCTGGGACAT AGAAGACAGC ATTAGTCATG AAACAGCTGA TGAAAAGGTG 
ATTTTTGGAA TTGAATTTAA TTCAACCTTT TTGGAGTGTA TACCTAAATC CCAACAAGCC 
TCTGTTGAGT GGTACATCCA GCGGTCAGGA GATGAGCATC GAGAGGAGTT GAAACCTGAT 
GAAAGGATCA TCAAAACTGA CTATGGGCTA CTGATTCGAA GTCTGCAGAA GAAGGATTCT 
GGGATGTATT ACTGCAAAGC ACAGGAGCAC ACTTTCATCC ACACCATAGT GAAGCTGACT 
TTGAATGTCA TTGAGAATGA ACAGATGGAA AATACCCAGA GGGCAGAATA CCAGGAGGGG 
CAGGTCAAGG ATCTGTTGGC TGAGTCACGG TTGAGATACA AAGACTACAT CCAAATCCTT 
AGCAGCCCGA ACTTCAGCCT GGACCAGTAC TGTGAGCAGA TGTGGTACAA GGAGAAGCGG 
AGACAGCGCA ACAAGGGCAG CCCAAAGTGG AAGCACATGC AGGAAATGAA GAAGAAACGA 
AATCGACGAC ATCACAGAGA CCTCGATGAG CTCCAGAGAT CAGTAGCTAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083158.3
CDS	547..2880
Translation	MNVTKDENPRSRSQDLHLFHAWMMLIMTVLFLPVTETSKQNIPRLKLTYKDLLLSNTCIPFLGSSEGLDFQTLLLDEERGILLLGAKDHVFLLSLVDLNKNFKKIYWPAAKERVELCKLAGKDANAECANFIRVLQPYNKTHVYVCGTGAFHPLCGYIDLGANKEELIFKLDTHNLESGRLKCPFDPQQPFASVMTDEHLYSGTASDFLGKDTAFTRSLGLMQDHHSIRTDISEHHWLNGAKFIGTFPIPDTYNPDDDKIYFFFRESSQEGSTSDRSILSRVGRVCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTHFDELQDIYLLPTRDERNPVVYGVFTTTSSIFKGSAVCVYSMADIRAVFNGPYAHKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISFIRRHPVMYKSVYPVAGAPTFKRINVDYRLTQIVVDHVVAEDGQYDVMFLGTDIGTVLKVVSISKEKWNMEEVVLEELQVFKHPTAILNMELSLKQQQLYVGSWDGLVQLSLHRCDTYGKACADCCLARDPYCAWDGNACSRYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFLECIPKSQQASVEWYIQRSGDEHREELKPDERIIKTDYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEYQEGQVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQMWYKEKRRQRNKGSPKWKHMQEMKKKRNRRHHRDLDELQRSVAT

Target ORF information:

RefSeq Version	NM_028882.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028882.4

ORF Insert Sequence:

ATGAATGTTA CTAAAGATGA GAACCCAAGA TCCAGAAGTC AAGATCTTCA CCTTTTTCAT 
GCTTGGATGA TGTTAATCAT GACGGTGCTC TTTCTTCCTG TCACTGAAAC GTCTAAACAA 
AATATTCCAA GACTCAAGCT AACCTACAAA GACTTGCTGC TTTCAAACAC CTGTATCCCC 
TTTTTGGGTT CATCAGAAGG ATTGGATTTC CAGACTCTTC TTTTGGATGA GGAGAGGGGC 
ATACTGCTCC TAGGAGCCAA AGACCATGTC TTCCTGCTCA GTCTGGTTGA CTTGAACAAG 
AATTTTAAGA AGATTTATTG GCCTGCTGCA AAAGAACGAG TGGAGCTATG TAAATTAGCT 
GGGAAAGATG CCAATGCAGA ATGTGCAAAT TTCATCCGTG TGCTTCAACC CTATAATAAG 
ACTCACGTTT ACGTGTGTGG AACTGGAGCG TTTCATCCGC TGTGTGGGTA CATTGATCTC 
GGCGCCAACA AGGAGGAACT CATATTTAAA CTAGACACGC ACAACCTGGA GTCTGGCAGA 
CTGAAATGTC CCTTTGATCC TCAGCAGCCT TTTGCTTCAG TAATGACAGA TGAGCACCTC 
TACTCTGGAA CAGCTTCTGA TTTCCTTGGC AAAGACACTG CATTCACAAG GTCTCTGGGG 
CTAATGCAGG ACCACCATTC CATCAGAACT GACATTTCAG AGCACCACTG GCTCAATGGA 
GCAAAATTTA TCGGAACATT CCCCATTCCA GACACCTATA ATCCAGATGA TGATAAAATA 
TATTTCTTCT TTCGAGAATC ATCCCAGGAA GGCAGTACTT CTGACAGAAG CATTCTTTCA 
AGAGTTGGAA GAGTTTGTAA GAATGATGTA GGTGGGCAAC GAAGTCTGAT AAACAAATGG 
ACAACTTTTC TAAAGGCAAG ACTGATTTGC TCGATTCCTG GAAGCGATGG GGCAGATACC 
CATTTTGATG AACTCCAAGA TATTTACTTA CTCCCTACGA GAGATGAAAG AAATCCTGTA 
GTATATGGAG TCTTTACCAC AACCAGCTCC ATCTTCAAAG GCTCTGCTGT CTGTGTGTAC 
AGCATGGCTG ATATCCGAGC AGTCTTTAAT GGTCCCTATG CTCATAAGGA AAGTGCTGAC 
CATCGCTGGG TGCAATATGA TGGAAGGATA CCTTACCCCC GACCTGGAAC GTGTCCAAGC 
AAAACCTATG ACCCACTGAT TAAGTCCACC CGAGACTTTC CAGACGATGT TATCAGTTTC 
ATAAGGCGGC ACCCTGTGAT GTATAAGTCC GTGTACCCAG TGGCAGGAGC ACCGACCTTC 
AAGAGAATCA ACGTGGATTA CAGACTGACG CAGATAGTGG TGGATCACGT GGTCGCTGAA 
GACGGGCAGT ATGATGTCAT GTTTCTCGGA ACAGACATTG GAACAGTCCT GAAAGTTGTG 
AGCATCTCCA AGGAGAAGTG GAATATGGAA GAGGTCGTAC TGGAGGAGCT TCAGGTATTC 
AAGCACCCAA CAGCTATCTT GAACATGGAG TTGTCGCTGA AGCAGCAACA GTTGTACGTT 
GGTTCCTGGG ATGGATTGGT CCAGCTCTCC TTGCACAGAT GCGACACTTA CGGGAAAGCA 
TGTGCAGACT GCTGTCTCGC CAGAGACCCT TACTGTGCCT GGGATGGAAA TGCTTGCTCC 
AGATATGCAC CCACGTCTAA AAGGCGAGCT AGACGCCAGG ATGTAAAATA TGGGGACCCA 
ATCACTCAGT GCTGGGACAT AGAAGACAGC ATTAGTCATG AAACAGCTGA TGAAAAGGTG 
ATTTTTGGAA TTGAATTTAA TTCAACCTTT TTGGAGTGTA TACCTAAATC CCAACAAGCC 
TCTGTTGAGT GGTACATCCA GCGGTCAGGA GATGAGCATC GAGAGGAGTT GAAACCTGAT 
GAAAGGATCA TCAAAACTGA CTATGGGCTA CTGATTCGAA GTCTGCAGAA GAAGGATTCT 
GGGATGTATT ACTGCAAAGC ACAGGAGCAC ACTTTCATCC ACACCATAGT GAAGCTGACT 
TTGAATGTCA TTGAGAATGA ACAGATGGAA AATACCCAGA GGGCAGAATA CCAGGAGGGG 
CAGGTCAAGG ATCTGTTGGC TGAGTCACGG TTGAGATACA AAGACTACAT CCAAATCCTT 
AGCAGCCCGA ACTTCAGCCT GGACCAGTAC TGTGAGCAGA TGTGGTACAA GGAGAAGCGG 
AGACAGCGCA ACAAGGGCAG CCCAAAGTGG AAGCACATGC AGGAAATGAA GAAGAAACGA 
AATCGACGAC ATCACAGAGA CCTCGATGAG CTCCAGAGAT CAGTAGCTAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema3d ( XM_030254046.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109906.1 Mus musculus sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19054
Clone ID Related Accession (Same CDS sequence)	XM_030254046.1 , NM_028882.4
Accession Version	XM_030254046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-3D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTTA CTAAAGATGA GAACCCAAGA TCCAGAAGTC AAGATCTTCA CCTTTTTCAT 
GCTTGGATGA TGTTAATCAT GACGGTGCTC TTTCTTCCTG TCACTGAAAC GTCTAAACAA 
AATATTCCAA GACTCAAGCT AACCTACAAA GACTTGCTGC TTTCAAACAC CTGTATCCCC 
TTTTTGGGTT CATCAGAAGG ATTGGATTTC CAGACTCTTC TTTTGGATGA GGAGAGGGGC 
ATACTGCTCC TAGGAGCCAA AGACCATGTC TTCCTGCTCA GTCTGGTTGA CTTGAACAAG 
AATTTTAAGA AGATTTATTG GCCTGCTGCA AAAGAACGAG TGGAGCTATG TAAATTAGCT 
GGGAAAGATG CCAATGCAGA ATGTGCAAAT TTCATCCGTG TGCTTCAACC CTATAATAAG 
ACTCACGTTT ACGTGTGTGG AACTGGAGCG TTTCATCCGC TGTGTGGGTA CATTGATCTC 
GGCGCCAACA AGGAGGAACT CATATTTAAA CTAGACACGC ACAACCTGGA GTCTGGCAGA 
CTGAAATGTC CCTTTGATCC TCAGCAGCCT TTTGCTTCAG TAATGACAGA TGAGCACCTC 
TACTCTGGAA CAGCTTCTGA TTTCCTTGGC AAAGACACTG CATTCACAAG GTCTCTGGGG 
CTAATGCAGG ACCACCATTC CATCAGAACT GACATTTCAG AGCACCACTG GCTCAATGGA 
GCAAAATTTA TCGGAACATT CCCCATTCCA GACACCTATA ATCCAGATGA TGATAAAATA 
TATTTCTTCT TTCGAGAATC ATCCCAGGAA GGCAGTACTT CTGACAGAAG CATTCTTTCA 
AGAGTTGGAA GAGTTTGTAA GAATGATGTA GGTGGGCAAC GAAGTCTGAT AAACAAATGG 
ACAACTTTTC TAAAGGCAAG ACTGATTTGC TCGATTCCTG GAAGCGATGG GGCAGATACC 
CATTTTGATG AACTCCAAGA TATTTACTTA CTCCCTACGA GAGATGAAAG AAATCCTGTA 
GTATATGGAG TCTTTACCAC AACCAGCTCC ATCTTCAAAG GCTCTGCTGT CTGTGTGTAC 
AGCATGGCTG ATATCCGAGC AGTCTTTAAT GGTCCCTATG CTCATAAGGA AAGTGCTGAC 
CATCGCTGGG TGCAATATGA TGGAAGGATA CCTTACCCCC GACCTGGAAC GTGTCCAAGC 
AAAACCTATG ACCCACTGAT TAAGTCCACC CGAGACTTTC CAGACGATGT TATCAGTTTC 
ATAAGGCGGC ACCCTGTGAT GTATAAGTCC GTGTACCCAG TGGCAGGAGC ACCGACCTTC 
AAGAGAATCA ACGTGGATTA CAGACTGACG CAGATAGTGG TGGATCACGT GGTCGCTGAA 
GACGGGCAGT ATGATGTCAT GTTTCTCGGA ACAGACATTG GAACAGTCCT GAAAGTTGTG 
AGCATCTCCA AGGAGAAGTG GAATATGGAA GAGGTCGTAC TGGAGGAGCT TCAGGTATTC 
AAGCACCCAA CAGCTATCTT GAACATGGAG TTGTCGCTGA AGCAGCAACA GTTGTACGTT 
GGTTCCTGGG ATGGATTGGT CCAGCTCTCC TTGCACAGAT GCGACACTTA CGGGAAAGCA 
TGTGCAGACT GCTGTCTCGC CAGAGACCCT TACTGTGCCT GGGATGGAAA TGCTTGCTCC 
AGATATGCAC CCACGTCTAA AAGGCGAGCT AGACGCCAGG ATGTAAAATA TGGGGACCCA 
ATCACTCAGT GCTGGGACAT AGAAGACAGC ATTAGTCATG AAACAGCTGA TGAAAAGGTG 
ATTTTTGGAA TTGAATTTAA TTCAACCTTT TTGGAGTGTA TACCTAAATC CCAACAAGCC 
TCTGTTGAGT GGTACATCCA GCGGTCAGGA GATGAGCATC GAGAGGAGTT GAAACCTGAT 
GAAAGGATCA TCAAAACTGA CTATGGGCTA CTGATTCGAA GTCTGCAGAA GAAGGATTCT 
GGGATGTATT ACTGCAAAGC ACAGGAGCAC ACTTTCATCC ACACCATAGT GAAGCTGACT 
TTGAATGTCA TTGAGAATGA ACAGATGGAA AATACCCAGA GGGCAGAATA CCAGGAGGGG 
CAGGTCAAGG ATCTGTTGGC TGAGTCACGG TTGAGATACA AAGACTACAT CCAAATCCTT 
AGCAGCCCGA ACTTCAGCCT GGACCAGTAC TGTGAGCAGA TGTGGTACAA GGAGAAGCGG 
AGACAGCGCA ACAAGGGCAG CCCAAAGTGG AAGCACATGC AGGAAATGAA GAAGAAACGA 
AATCGACGAC ATCACAGAGA CCTCGATGAG CTCCAGAGAT CAGTAGCTAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109906.1
CDS	671..3004
Translation	MNVTKDENPRSRSQDLHLFHAWMMLIMTVLFLPVTETSKQNIPRLKLTYKDLLLSNTCIPFLGSSEGLDFQTLLLDEERGILLLGAKDHVFLLSLVDLNKNFKKIYWPAAKERVELCKLAGKDANAECANFIRVLQPYNKTHVYVCGTGAFHPLCGYIDLGANKEELIFKLDTHNLESGRLKCPFDPQQPFASVMTDEHLYSGTASDFLGKDTAFTRSLGLMQDHHSIRTDISEHHWLNGAKFIGTFPIPDTYNPDDDKIYFFFRESSQEGSTSDRSILSRVGRVCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTHFDELQDIYLLPTRDERNPVVYGVFTTTSSIFKGSAVCVYSMADIRAVFNGPYAHKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISFIRRHPVMYKSVYPVAGAPTFKRINVDYRLTQIVVDHVVAEDGQYDVMFLGTDIGTVLKVVSISKEKWNMEEVVLEELQVFKHPTAILNMELSLKQQQLYVGSWDGLVQLSLHRCDTYGKACADCCLARDPYCAWDGNACSRYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFLECIPKSQQASVEWYIQRSGDEHREELKPDERIIKTDYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEYQEGQVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQMWYKEKRRQRNKGSPKWKHMQEMKKKRNRRHHRDLDELQRSVAT

Target ORF information:

RefSeq Version	XM_030254046.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254046.1

ORF Insert Sequence:

ATGAATGTTA CTAAAGATGA GAACCCAAGA TCCAGAAGTC AAGATCTTCA CCTTTTTCAT 
GCTTGGATGA TGTTAATCAT GACGGTGCTC TTTCTTCCTG TCACTGAAAC GTCTAAACAA 
AATATTCCAA GACTCAAGCT AACCTACAAA GACTTGCTGC TTTCAAACAC CTGTATCCCC 
TTTTTGGGTT CATCAGAAGG ATTGGATTTC CAGACTCTTC TTTTGGATGA GGAGAGGGGC 
ATACTGCTCC TAGGAGCCAA AGACCATGTC TTCCTGCTCA GTCTGGTTGA CTTGAACAAG 
AATTTTAAGA AGATTTATTG GCCTGCTGCA AAAGAACGAG TGGAGCTATG TAAATTAGCT 
GGGAAAGATG CCAATGCAGA ATGTGCAAAT TTCATCCGTG TGCTTCAACC CTATAATAAG 
ACTCACGTTT ACGTGTGTGG AACTGGAGCG TTTCATCCGC TGTGTGGGTA CATTGATCTC 
GGCGCCAACA AGGAGGAACT CATATTTAAA CTAGACACGC ACAACCTGGA GTCTGGCAGA 
CTGAAATGTC CCTTTGATCC TCAGCAGCCT TTTGCTTCAG TAATGACAGA TGAGCACCTC 
TACTCTGGAA CAGCTTCTGA TTTCCTTGGC AAAGACACTG CATTCACAAG GTCTCTGGGG 
CTAATGCAGG ACCACCATTC CATCAGAACT GACATTTCAG AGCACCACTG GCTCAATGGA 
GCAAAATTTA TCGGAACATT CCCCATTCCA GACACCTATA ATCCAGATGA TGATAAAATA 
TATTTCTTCT TTCGAGAATC ATCCCAGGAA GGCAGTACTT CTGACAGAAG CATTCTTTCA 
AGAGTTGGAA GAGTTTGTAA GAATGATGTA GGTGGGCAAC GAAGTCTGAT AAACAAATGG 
ACAACTTTTC TAAAGGCAAG ACTGATTTGC TCGATTCCTG GAAGCGATGG GGCAGATACC 
CATTTTGATG AACTCCAAGA TATTTACTTA CTCCCTACGA GAGATGAAAG AAATCCTGTA 
GTATATGGAG TCTTTACCAC AACCAGCTCC ATCTTCAAAG GCTCTGCTGT CTGTGTGTAC 
AGCATGGCTG ATATCCGAGC AGTCTTTAAT GGTCCCTATG CTCATAAGGA AAGTGCTGAC 
CATCGCTGGG TGCAATATGA TGGAAGGATA CCTTACCCCC GACCTGGAAC GTGTCCAAGC 
AAAACCTATG ACCCACTGAT TAAGTCCACC CGAGACTTTC CAGACGATGT TATCAGTTTC 
ATAAGGCGGC ACCCTGTGAT GTATAAGTCC GTGTACCCAG TGGCAGGAGC ACCGACCTTC 
AAGAGAATCA ACGTGGATTA CAGACTGACG CAGATAGTGG TGGATCACGT GGTCGCTGAA 
GACGGGCAGT ATGATGTCAT GTTTCTCGGA ACAGACATTG GAACAGTCCT GAAAGTTGTG 
AGCATCTCCA AGGAGAAGTG GAATATGGAA GAGGTCGTAC TGGAGGAGCT TCAGGTATTC 
AAGCACCCAA CAGCTATCTT GAACATGGAG TTGTCGCTGA AGCAGCAACA GTTGTACGTT 
GGTTCCTGGG ATGGATTGGT CCAGCTCTCC TTGCACAGAT GCGACACTTA CGGGAAAGCA 
TGTGCAGACT GCTGTCTCGC CAGAGACCCT TACTGTGCCT GGGATGGAAA TGCTTGCTCC 
AGATATGCAC CCACGTCTAA AAGGCGAGCT AGACGCCAGG ATGTAAAATA TGGGGACCCA 
ATCACTCAGT GCTGGGACAT AGAAGACAGC ATTAGTCATG AAACAGCTGA TGAAAAGGTG 
ATTTTTGGAA TTGAATTTAA TTCAACCTTT TTGGAGTGTA TACCTAAATC CCAACAAGCC 
TCTGTTGAGT GGTACATCCA GCGGTCAGGA GATGAGCATC GAGAGGAGTT GAAACCTGAT 
GAAAGGATCA TCAAAACTGA CTATGGGCTA CTGATTCGAA GTCTGCAGAA GAAGGATTCT 
GGGATGTATT ACTGCAAAGC ACAGGAGCAC ACTTTCATCC ACACCATAGT GAAGCTGACT 
TTGAATGTCA TTGAGAATGA ACAGATGGAA AATACCCAGA GGGCAGAATA CCAGGAGGGG 
CAGGTCAAGG ATCTGTTGGC TGAGTCACGG TTGAGATACA AAGACTACAT CCAAATCCTT 
AGCAGCCCGA ACTTCAGCCT GGACCAGTAC TGTGAGCAGA TGTGGTACAA GGAGAAGCGG 
AGACAGCGCA ACAAGGGCAG CCCAAAGTGG AAGCACATGC AGGAAATGAA GAAGAAACGA 
AATCGACGAC ATCACAGAGA CCTCGATGAG CTCCAGAGAT CAGTAGCTAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System.
Gastroenterology(2017 Oct)
Memic F,Knoflach V,Morarach K,Sadler R,Laranjeira C,Hjerling-Leffler J,Sundstr?m E,Pachnis V,Marklund U

Semaphorin signaling facilitates cleft formation in the developing salivary gland.
Development (Cambridge, England)134(16)2935-45(2007 Aug)
Chung L,Yang TL,Huang HR,Hsu SM,Cheng HJ,Huang PH

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Sema3d cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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