Eif2ak2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif2ak2
Entrez Gene ID	54287
Full Name	eukaryotic translation initiation factor 2-alpha kinase 2
Synonyms	Pkr,Prkr
General protein information	Preferred Names eukaryotic translation initiation factor 2-alpha kinase 2 Names interferon-induced, double-stranded RNA-activated protein kinase Protein kinase, interferon-inducible double stranded RNA dependent eIF-2A protein kinase 2 interferon-inducible RNA-dependent protein kinase protein kinase R protein kinase RNA-activated tyrosine-protein kinase EIF2AK2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q11
Summary	double-stranded RNA dependent eukaryotic initiation factor 2-kinase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764425.1	XP_008762647.1	interferon-induced, double-stranded RNA-activated protein kinase isoform X1
XM_008764426.2	XP_008762648.1	interferon-induced, double-stranded RNA-activated protein kinase isoform X2
NM_019335.1	NP_062208.1	interferon-induced, double-stranded RNA-activated protein kinase

Pathways from BioSystems

Homologies

	EIF2AK2	NP_001077417.1
Canis lupus familiaris (dog)	EIF2AK2	NP_001041600.1
Homo sapiens (human)	EIF2AK2	NP_002750.1
Pan troglodytes (chimpanzee)	EIF2AK2	NP_001138509.1
Gallus gallus (chicken)	EIF2AK2	NP_989818.1
Xenopus tropicalis (tropical clawed frog)	pkr3	XP_002933926.1
Bos taurus (cattle)	EIF2AK2	NP_835210.2
Rattus norvegicus (Norway rat)	Eif2ak2	NP_062208.1
Xenopus tropicalis (tropical clawed frog)	eif2ak2	NP_001072537.1
Mus musculus (house mouse)	Eif2ak2	NP_035293.1
Danio rerio (zebrafish)	eif2ak2	NP_001107942.1

Gene Ontology
Bibliography

Related articles in PubMed

PKR regulates LPS-induced osteoclast formation and bone destruction in vitro and in vivo.
Teramachi J, Inagaki Y, Shinohara H, Okamura H, Yang D, Ochiai K, Baba R, Morimoto H, Nagata T, Haneji T
Oral diseases23(2)181-188(2017 Mar)

PKR and HMGB1 expression and function in rheumatoid arthritis.
Wang WJ, Yin SJ, Rong RQ
Genetics and molecular research : GMR14(4)17864-70(2015 Dec)

PERK pathway is involved in oxygen-glucose-serum deprivation-induced NF-kB activation via ROS generation in spinal cord astrocytes.
Liu J, Du L
Biochemical and biophysical research communications467(2)197-203(2015 Nov)

Zinc regulates expression of IL-23 p19 mRNA via activation of eIF2?/ATF4 axis in HAPI cells.
Doi T, Hara H, Kajita M, Kamiya T, Adachi T
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine28(5)891-902(2015 Oct)

p58IPK is an inhibitor of the eIF2? kinase GCN2 and its localization and expression underpin protein synthesis and ER processing capacity.
Roobol A, Roobol J, Bastide A, Knight JR, Willis AE, Smales CM
The Biochemical journal465(2)213-25(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PKR plays a pivotal role in LPS-induced bone loss in periodontal disease.
Title: PKR regulates LPS-induced osteoclast formation and bone destruction in vitro and in vivo.

Increased levels of PKR and HMGB1 in synovium or blood appear to be involved in the occurrence and development ofrheumatoid arthritis (RA )in a rat model. Selective inhibition of PKR improves the symptoms of RA perhaps by inhibiting the release of HMGB1
Title: PKR and HMGB1 expression and function in rheumatoid arthritis.

PERK pathway is involved in oxygen-glucose-serum deprivation-induced NF-kappaB activation via reactive oxygen species generation in spinal cord astrocytes.
Title: PERK pathway is involved in oxygen-glucose-serum deprivation-induced NF-kB activation via ROS generation in spinal cord astrocytes.

Zn(2+) stimulated the phosphorylation of eIF2a and promoted the nuclear accumulation of activating transcription factor 4 (ATF4). Treatment with salubrinal enhanced Zn(2+)-induced ATF4 accumulation and IL-23 p19 mRNA expression.
Title: Zinc regulates expression of IL-23 p19 mRNA via activation of eIF2?/ATF4 axis in HAPI cells.

Taken together, these data suggest that PP1-12 protects cardiomyocytes from TM- and I/R-induced apoptosis, and this effect is achieved at least in part through the inhibition of cell apoptosis and the induction of eIF2alpha phosphorylation
Title: Inhibition of serine/threonine protein phosphatase PP1 protects cardiomyocytes from tunicamycin-induced apoptosis and I/R through the upregulation of p-eIF2?.

The following Eif2ak2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif2ak2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04456	NM_019335.1 Latest version!	Rattus norvegicus eukaryotic translation initiation factor 2-alpha kinase 2 (Eif2ak2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa43418	XM_008764425.1 Latest version!	Rattus norvegicus eukaryotic translation initiation factor 2-alpha kinase 2 (Eif2ak2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa43419	XM_008764426.2 Latest version!	Rattus norvegicus eukaryotic translation initiation factor 2-alpha kinase 2 (Eif2ak2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif2ak2 ( NM_019335.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062208.1 Rattus norvegicus eukaryotic translation initiation factor 2-alpha kinase 2 (Eif2ak2), mRNA.

Price & Availability↑

CloneID	ORa04456
Clone ID Related Accession (Same CDS sequence)	NM_019335.1
Accession Version	NM_019335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	interferon-induced, double-stranded RNA-activated protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L29281.1. On Dec 17, 2003 this sequence version replaced XM_346785.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L29281.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCCAGTG ATACACCAGG TTTCTACGTG GACAAACTTA ACAAATACTC CCAGATACAC 
AAAGTAAAGA TTATATATAA AGAAATTTCT GTTACAGGAC CTCCACACGA CAGAAGGTTT 
ACATTTCAAG TTATAATAGA AGAGAGGGAA TTTCCAGAAG GTGAAGGTAG ATCAAAGCAG 
GAGGCGAAAA ATAATGCTGC CAAATTAGCT GTCGAAATAC TTGATAATGA AAACAAGGTG 
GATAGTCACA CGGATGCTTC TGAACAAGGT TTAATAGAGG GGAACTACAT TGGCCTTGTC 
AATTCTTTTG CCCAGAAGGA AAATCTGCCT GTAAATTTTG AACTGTGTGA CCCCGACTCC 
CAATTGCCTC ACAGATTTAT TTGTAAATGC AAAATCGGGC AGACTACGTA TGGTACTGGT 
TTCGGTGCTA ACAAAAAGGA GGCAAAGCAG TTGGCTGCCA AAAATGCGTA TCAGAAGCTG 
TCAGAGAAAA GCCCATCGAA AACTGGCTTT GTCACATCTT TATCCAGTGA CTTTTCCAGC 
AGCTCGTCTA TAACAAGTAA CTCTGCTTCT CAGTCAGCAT CTGGAAGGGA TTTCGAGGAC 
ATATTTATGA ATGGTCTCAG AGAAAAAAGG AAATCAGGAG TAAAAGTGCC ATCTGATGAC 
GTGCTAAGAA ATAAATATAC CTTGGACGAC AGGTTTAGCA AAGATTTTGA AGACATAGAA 
GAAATTGGCT CGGGTGGATT TGGCCAAGTT TTCAAAGCAA AACACAGAAT CGATGGAAAG 
ACGTATGCTA TTAAGCGCAT TACATATAAC ACCAAGAAGG CAAAGCGTGA AGTACAAGCA 
CTAGCAGAAC TCAATCACGC CAACATTGTT CAATACCGAG TTTGTTGGGA GGGAGAGGAC 
TATGACTATG ATCCCGAGAA CAGCACAAAC GGTGACACAA GTCGATACAA GACCCGGTGC 
CTCTTTATCC AAATGGAATT CTGTGATAAA GGAACTCTGC AGCAGTGGTT GGAAAAGAGA 
AATCGGAGTC AAGAGGACAA GGCTTTGGTT TTGGAGTTAT TTGAACAAAT AGTGACAGGA 
GTGGATTATA TACACTCGAA AGGTTTAATT CATAGAGACC TTAAGCCAGG TAATATATTT 
TTAGTGGATG AAAAACACAT TAAGATCGGA GACTTTGGCC TTGCAACAGC CCTGGAAAAT 
GATGGAAATC CTCGAACAAA GTATACAGGA ACTCCTCAAT ACATGAGTCC AGAACAAAAG 
TCATCGTTAG TGGAATATGG AAAGGAAGTG GACATCTTTG CTTTGGGCCT TATTCTAGCT 
GAACTTCTTC ACATATGCAA AACTGATTCA GAGAAAATAG AGTTTTTCCA ACTTCTAAGA 
AATGGCATCT TCTCCGATGA TATTTTCGAC AACAAGGAAA AAAGCCTTCT ACAGAAATTA 
CTCTCAAGTA AACCCAGGGA ACGACCCAAT ACGTCTGAAA TCCTGAAGAC TTTGGCTGAG 
TGGAAGAACA TCTCGGAGAA AAAGAAAAGA AACACGTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062208.1
CDS	151..1692
Translation	MASDTPGFYVDKLNKYSQIHKVKIIYKEISVTGPPHDRRFTFQVIIEEREFPEGEGRSKQEAKNNAAKLAVEILDNENKVDSHTDASEQGLIEGNYIGLVNSFAQKENLPVNFELCDPDSQLPHRFICKCKIGQTTYGTGFGANKKEAKQLAAKNAYQKLSEKSPSKTGFVTSLSSDFSSSSSITSNSASQSASGRDFEDIFMNGLREKRKSGVKVPSDDVLRNKYTLDDRFSKDFEDIEEIGSGGFGQVFKAKHRIDGKTYAIKRITYNTKKAKREVQALAELNHANIVQYRVCWEGEDYDYDPENSTNGDTSRYKTRCLFIQMEFCDKGTLQQWLEKRNRSQEDKALVLELFEQIVTGVDYIHSKGLIHRDLKPGNIFLVDEKHIKIGDFGLATALENDGNPRTKYTGTPQYMSPEQKSSLVEYGKEVDIFALGLILAELLHICKTDSEKIEFFQLLRNGIFSDDIFDNKEKSLLQKLLSSKPRERPNTSEILKTLAEWKNISEKKKRNTC

Target ORF information:

RefSeq Version	NM_019335.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic translation initiation factor 2-alpha kinase 2 (Eif2ak2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019335.1

ORF Insert Sequence:

ATGGCCAGTG ATACACCAGG TTTCTACGTG GACAAACTTA ACAAATACTC CCAGATACAC 
AAAGTAAAGA TTATATATAA AGAAATTTCT GTTACAGGAC CTCCACACGA CAGAAGGTTT 
ACATTTCAAG TTATAATAGA AGAGAGGGAA TTTCCAGAAG GTGAAGGTAG ATCAAAGCAG 
GAGGCGAAAA ATAATGCTGC CAAATTAGCT GTCGAAATAC TTGATAATGA AAACAAGGTG 
GATAGTCACA CGGATGCTTC TGAACAAGGT TTAATAGAGG GGAACTACAT TGGCCTTGTC 
AATTCTTTTG CCCAGAAGGA AAATCTGCCT GTAAATTTTG AACTGTGTGA CCCCGACTCC 
CAATTGCCTC ACAGATTTAT TTGTAAATGC AAAATCGGGC AGACTACGTA TGGTACTGGT 
TTCGGTGCTA ACAAAAAGGA GGCAAAGCAG TTGGCTGCCA AAAATGCGTA TCAGAAGCTG 
TCAGAGAAAA GCCCATCGAA AACTGGCTTT GTCACATCTT TATCCAGTGA CTTTTCCAGC 
AGCTCGTCTA TAACAAGTAA CTCTGCTTCT CAGTCAGCAT CTGGAAGGGA TTTCGAGGAC 
ATATTTATGA ATGGTCTCAG AGAAAAAAGG AAATCAGGAG TAAAAGTGCC ATCTGATGAC 
GTGCTAAGAA ATAAATATAC CTTGGACGAC AGGTTTAGCA AAGATTTTGA AGACATAGAA 
GAAATTGGCT CGGGTGGATT TGGCCAAGTT TTCAAAGCAA AACACAGAAT CGATGGAAAG 
ACGTATGCTA TTAAGCGCAT TACATATAAC ACCAAGAAGG CAAAGCGTGA AGTACAAGCA 
CTAGCAGAAC TCAATCACGC CAACATTGTT CAATACCGAG TTTGTTGGGA GGGAGAGGAC 
TATGACTATG ATCCCGAGAA CAGCACAAAC GGTGACACAA GTCGATACAA GACCCGGTGC 
CTCTTTATCC AAATGGAATT CTGTGATAAA GGAACTCTGC AGCAGTGGTT GGAAAAGAGA 
AATCGGAGTC AAGAGGACAA GGCTTTGGTT TTGGAGTTAT TTGAACAAAT AGTGACAGGA 
GTGGATTATA TACACTCGAA AGGTTTAATT CATAGAGACC TTAAGCCAGG TAATATATTT 
TTAGTGGATG AAAAACACAT TAAGATCGGA GACTTTGGCC TTGCAACAGC CCTGGAAAAT 
GATGGAAATC CTCGAACAAA GTATACAGGA ACTCCTCAAT ACATGAGTCC AGAACAAAAG 
TCATCGTTAG TGGAATATGG AAAGGAAGTG GACATCTTTG CTTTGGGCCT TATTCTAGCT 
GAACTTCTTC ACATATGCAA AACTGATTCA GAGAAAATAG AGTTTTTCCA ACTTCTAAGA 
AATGGCATCT TCTCCGATGA TATTTTCGAC AACAAGGAAA AAAGCCTTCT ACAGAAATTA 
CTCTCAAGTA AACCCAGGGA ACGACCCAAT ACGTCTGAAA TCCTGAAGAC TTTGGCTGAG 
TGGAAGAACA TCTCGGAGAA AAAGAAAAGA AACACGTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif2ak2 ( XM_008764425.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762647.1 Rattus norvegicus eukaryotic translation initiation factor 2-alpha kinase 2 (Eif2ak2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43418
Clone ID Related Accession (Same CDS sequence)	XM_008764425.1
Accession Version	XM_008764425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	interferon-induced, double-stranded RNA-activated protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTCA GTGTCACATG TGTAGGTGCC TCTTCCTTTG CAGGTCACCG GGGAAGAAAA 
ATGGCCAGTG ATACACCAGG TTTCTACGTG GACAAACTTA ACAAATACTC CCAGATACAC 
AAAGTAAAGA TTATATATAA AGAAATTTCT GTTACAGGAC CTCCACACGA CAGAAGGTTT 
ACATTTCAAG TTATAATAGA AGAGAGGGAA TTTCCAGAAG GTGAAGGTAG ATCAAAGCAG 
GAGGCGAAAA ATAATGCTGC CAAATTAGCT GTCGAAATAC TTGATAATGA AAACAAGGTG 
GATAGTCACA CGGATGCTTC TGAACAAGGT TTAATAGAGG GGAACTACAT TGGCCTTGTC 
AATTCTTTTG CCCAGAAGGA AAATCTGCCT GTAAATTTTG AACTGTGTGA CCCCGACTCC 
CAATTGCCTC ACAGATTTAT TTGTAAATGC AAAATCGGGC AGACTACGTA TGGTACTGGT 
TTTGGCGCTA ACAAAAAGGA GGCAAAGCAG TTGGCTGCCA AAAATGCGTA TCAGAAGCTG 
TCAGAGAAAA GCCCATCGAA AACTGGCTTT GTCACATCTT TATCCAGTGA CTTTTCCAGC 
AGCTCGTCTA TAACAAGTAA CTCTGCTTCT CAGTCAGCAT CTGGAAGGGA TTTCGAGGAC 
ATATTTATGA ATGGTCTCAG AGAAAAAAGG AAATCAGGAG TAAAAGTGCC ATCTGATGAC 
GTGCTAAGAA ATAAATATAC CTTGGACGAC AGGTTTAGCA AAGATTTTGA AGACATAGAA 
GAAATTGGCT CGGGTGGATT TGGCCAAGTT TTCAAAGCAA AACACAGAAT CGATGGAAAG 
ACGTATGCTA TTAAGCGCAT TACATATAAC ACCAAGAAGG CAAAGCGTGA AGTACAAGCA 
CTAGCAGAAC TCAATCACGC CAACATTGTT CAATACCGAG TTTGTTGGGA GGGAGAGGAC 
TATGACTATG ATCCCGAGAA CAGCACAAAC GGTGACACAA GTCGATACAA GACCCGGTGC 
CTCTTTATCC AAATGGAATT CTGTGATAAA GGAACTCTGC AGCAGTGGTT GGAAAAGAGA 
AATCGGAGTC AAGAGGACAA GGCTTTGGTT TTGGAGTTAT TTGAACAAAT AGTGACAGGA 
GTGGATTATA TACACTCGAA AGGTTTAATT CATAGAGACC TTAAGCCAGG TAATATATTT 
TTAGTGGATG AAAAACACAT TAAGATCGGA GACTTTGGCC TTGCAACAGC CCTGGAAAAT 
GATGGAAATC CTCGAACAAA GTATACAGGA ACTCCTCAAT ACATGAGTCC AGAACAAAAG 
TCATCGTTAG TGGAATATGG AAAGGAAGTG GACATCTTTG CTTTGGGCCT TATTCTAGCT 
GAACTTCTTC ACATATGCAA AACTGATTCA GAGAAAATAG AGTTTTTCCA ACTTCTAAGA 
AATGGCGTCT TCTCCGATGA TATTTTCGAC AACAAGGAAA AAAGTCTTCT ACAGAAATTA 
CTCTCAAGTA AACCCAGGGA ACGACCCAAT ACGTCTGAAA TCCTGAAGAC TTTGGCTGAG 
TGGAAGAACA TCTCGGAGAA AAAGAAAAGA AACACGTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762647.1
CDS	315..1916
Translation	MSVSVTCVGASSFAGHRGRKMASDTPGFYVDKLNKYSQIHKVKIIYKEISVTGPPHDRRFTFQVIIEEREFPEGEGRSKQEAKNNAAKLAVEILDNENKVDSHTDASEQGLIEGNYIGLVNSFAQKENLPVNFELCDPDSQLPHRFICKCKIGQTTYGTGFGANKKEAKQLAAKNAYQKLSEKSPSKTGFVTSLSSDFSSSSSITSNSASQSASGRDFEDIFMNGLREKRKSGVKVPSDDVLRNKYTLDDRFSKDFEDIEEIGSGGFGQVFKAKHRIDGKTYAIKRITYNTKKAKREVQALAELNHANIVQYRVCWEGEDYDYDPENSTNGDTSRYKTRCLFIQMEFCDKGTLQQWLEKRNRSQEDKALVLELFEQIVTGVDYIHSKGLIHRDLKPGNIFLVDEKHIKIGDFGLATALENDGNPRTKYTGTPQYMSPEQKSSLVEYGKEVDIFALGLILAELLHICKTDSEKIEFFQLLRNGVFSDDIFDNKEKSLLQKLLSSKPRERPNTSEILKTLAEWKNISEKKKRNTC

Target ORF information:

RefSeq Version	XM_008764425.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic translation initiation factor 2-alpha kinase 2 (Eif2ak2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764425.1

ORF Insert Sequence:

ATGTCTGTCA GTGTCACATG TGTAGGTGCC TCTTCCTTTG CAGGTCACCG GGGAAGAAAA 
ATGGCCAGTG ATACACCAGG TTTCTACGTG GACAAACTTA ACAAATACTC CCAGATACAC 
AAAGTAAAGA TTATATATAA AGAAATTTCT GTTACAGGAC CTCCACACGA CAGAAGGTTT 
ACATTTCAAG TTATAATAGA AGAGAGGGAA TTTCCAGAAG GTGAAGGTAG ATCAAAGCAG 
GAGGCGAAAA ATAATGCTGC CAAATTAGCT GTCGAAATAC TTGATAATGA AAACAAGGTG 
GATAGTCACA CGGATGCTTC TGAACAAGGT TTAATAGAGG GGAACTACAT TGGCCTTGTC 
AATTCTTTTG CCCAGAAGGA AAATCTGCCT GTAAATTTTG AACTGTGTGA CCCCGACTCC 
CAATTGCCTC ACAGATTTAT TTGTAAATGC AAAATCGGGC AGACTACGTA TGGTACTGGT 
TTTGGCGCTA ACAAAAAGGA GGCAAAGCAG TTGGCTGCCA AAAATGCGTA TCAGAAGCTG 
TCAGAGAAAA GCCCATCGAA AACTGGCTTT GTCACATCTT TATCCAGTGA CTTTTCCAGC 
AGCTCGTCTA TAACAAGTAA CTCTGCTTCT CAGTCAGCAT CTGGAAGGGA TTTCGAGGAC 
ATATTTATGA ATGGTCTCAG AGAAAAAAGG AAATCAGGAG TAAAAGTGCC ATCTGATGAC 
GTGCTAAGAA ATAAATATAC CTTGGACGAC AGGTTTAGCA AAGATTTTGA AGACATAGAA 
GAAATTGGCT CGGGTGGATT TGGCCAAGTT TTCAAAGCAA AACACAGAAT CGATGGAAAG 
ACGTATGCTA TTAAGCGCAT TACATATAAC ACCAAGAAGG CAAAGCGTGA AGTACAAGCA 
CTAGCAGAAC TCAATCACGC CAACATTGTT CAATACCGAG TTTGTTGGGA GGGAGAGGAC 
TATGACTATG ATCCCGAGAA CAGCACAAAC GGTGACACAA GTCGATACAA GACCCGGTGC 
CTCTTTATCC AAATGGAATT CTGTGATAAA GGAACTCTGC AGCAGTGGTT GGAAAAGAGA 
AATCGGAGTC AAGAGGACAA GGCTTTGGTT TTGGAGTTAT TTGAACAAAT AGTGACAGGA 
GTGGATTATA TACACTCGAA AGGTTTAATT CATAGAGACC TTAAGCCAGG TAATATATTT 
TTAGTGGATG AAAAACACAT TAAGATCGGA GACTTTGGCC TTGCAACAGC CCTGGAAAAT 
GATGGAAATC CTCGAACAAA GTATACAGGA ACTCCTCAAT ACATGAGTCC AGAACAAAAG 
TCATCGTTAG TGGAATATGG AAAGGAAGTG GACATCTTTG CTTTGGGCCT TATTCTAGCT 
GAACTTCTTC ACATATGCAA AACTGATTCA GAGAAAATAG AGTTTTTCCA ACTTCTAAGA 
AATGGCGTCT TCTCCGATGA TATTTTCGAC AACAAGGAAA AAAGTCTTCT ACAGAAATTA 
CTCTCAAGTA AACCCAGGGA ACGACCCAAT ACGTCTGAAA TCCTGAAGAC TTTGGCTGAG 
TGGAAGAACA TCTCGGAGAA AAAGAAAAGA AACACGTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif2ak2 ( XM_008764426.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762648.1 Rattus norvegicus eukaryotic translation initiation factor 2-alpha kinase 2 (Eif2ak2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa43419
Clone ID Related Accession (Same CDS sequence)	XM_008764426.2
Accession Version	XM_008764426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	interferon-induced, double-stranded RNA-activated protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGTG ATACACCAGG TTTCTACGTG GACAAACTTA ACAAATACTC CCAGATACAC 
AAAGTAAAGA TTATATATAA AGAAATTTCT GTTACAGGAC CTCCACACGA CAGAAGGTTT 
ACATTTCAAG TTATAATAGA AGAGAGGGAA TTTCCAGAAG GTGAAGGTAG ATCAAAGCAG 
GAGGCGAAAA ATAATGCTGC CAAATTAGCT GTCGAAATAC TTGATAATGA AAACAAGGTG 
GATAGTCACA CGGATGCTTC TGAACAAGGT TTAATAGAGG GGAACTACAT TGGCCTTGTC 
AATTCTTTTG CCCAGAAGGA AAATCTGCCT GTAAATTTTG AACTGTGTGA CCCCGACTCC 
CAATTGCCTC ACAGATTTAT TTGTAAATGC AAAATCGGGC AGACTACGTA TGGTACTGGT 
TTTGGCGCTA ACAAAAAGGA GGCAAAGCAG TTGGCTGCCA AAAATGCGTA TCAGAAGCTG 
TCAGAGAAAA GCCCATCGAA AACTGGCTTT GTCACATCTT TATCCAGTGA CTTTTCCAGC 
AGCTCGTCTA TAACAAGTAA CTCTGCTTCT CAGTCAGCAT CTGGAAGGGA TTTCGAGGAC 
ATATTTATGA ATGGTCTCAG AGAAAAAAGG AAATCAGGAG TAAAAGTGCC ATCTGATGAC 
GTGCTAAGAA ATAAATATAC CTTGGACGAC AGGTTTAGCA AAGATTTTGA AGACATAGAA 
GAAATTGGCT CGGGTGGATT TGGCCAAGTT TTCAAAGCAA AACACAGAAT CGATGGAAAG 
ACGTATGCTA TTAAGCGCAT TACATATAAC ACCAAGAAGG CAAAGCGTGA AGTACAAGCA 
CTAGCAGAAC TCAATCACGC CAACATTGTT CAATACCGAG TTTGTTGGGA GGGAGAGGAC 
TATGACTATG ATCCCGAGAA CAGCACAAAC GGTGACACAA GTCGATACAA GACCCGGTGC 
CTCTTTATCC AAATGGAATT CTGTGATAAA GGAACTCTGC AGCAGTGGTT GGAAAAGAGA 
AATCGGAGTC AAGAGGACAA GGCTTTGGTT TTGGAGTTAT TTGAACAAAT AGTGACAGGA 
GTGGATTATA TACACTCGAA AGGTTTAATT CATAGAGACC TTAAGCCAGG TAATATATTT 
TTAGTGGATG AAAAACACAT TAAGATCGGA GACTTTGGCC TTGCAACAGC CCTGGAAAAT 
GATGGAAATC CTCGAACAAA GTATACAGGA ACTCCTCAAT ACATGAGTCC AGAACAAAAG 
TCATCGTTAG TGGAATATGG AAAGGAAGTG GACATCTTTG CTTTGGGCCT TATTCTAGCT 
GAACTTCTTC ACATATGCAA AACTGATTCA GAGAAAATAG AGTTTTTCCA ACTTCTAAGA 
AATGGCGTCT TCTCCGATGA TATTTTCGAC AACAAGGAAA AAAGTCTTCT ACAGAAATTA 
CTCTCAAGTA AACCCAGGGA ACGACCCAAT ACGTCTGAAA TCCTGAAGAC TTTGGCTGAG 
TGGAAGAACA TCTCGGAGAA AAAGAAAAGA AACACGTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762648.1
CDS	302..1843
Translation	MASDTPGFYVDKLNKYSQIHKVKIIYKEISVTGPPHDRRFTFQVIIEEREFPEGEGRSKQEAKNNAAKLAVEILDNENKVDSHTDASEQGLIEGNYIGLVNSFAQKENLPVNFELCDPDSQLPHRFICKCKIGQTTYGTGFGANKKEAKQLAAKNAYQKLSEKSPSKTGFVTSLSSDFSSSSSITSNSASQSASGRDFEDIFMNGLREKRKSGVKVPSDDVLRNKYTLDDRFSKDFEDIEEIGSGGFGQVFKAKHRIDGKTYAIKRITYNTKKAKREVQALAELNHANIVQYRVCWEGEDYDYDPENSTNGDTSRYKTRCLFIQMEFCDKGTLQQWLEKRNRSQEDKALVLELFEQIVTGVDYIHSKGLIHRDLKPGNIFLVDEKHIKIGDFGLATALENDGNPRTKYTGTPQYMSPEQKSSLVEYGKEVDIFALGLILAELLHICKTDSEKIEFFQLLRNGVFSDDIFDNKEKSLLQKLLSSKPRERPNTSEILKTLAEWKNISEKKKRNTC

Target ORF information:

RefSeq Version	XM_008764426.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic translation initiation factor 2-alpha kinase 2 (Eif2ak2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764426.2

ORF Insert Sequence:

ATGGCCAGTG ATACACCAGG TTTCTACGTG GACAAACTTA ACAAATACTC CCAGATACAC 
AAAGTAAAGA TTATATATAA AGAAATTTCT GTTACAGGAC CTCCACACGA CAGAAGGTTT 
ACATTTCAAG TTATAATAGA AGAGAGGGAA TTTCCAGAAG GTGAAGGTAG ATCAAAGCAG 
GAGGCGAAAA ATAATGCTGC CAAATTAGCT GTCGAAATAC TTGATAATGA AAACAAGGTG 
GATAGTCACA CGGATGCTTC TGAACAAGGT TTAATAGAGG GGAACTACAT TGGCCTTGTC 
AATTCTTTTG CCCAGAAGGA AAATCTGCCT GTAAATTTTG AACTGTGTGA CCCCGACTCC 
CAATTGCCTC ACAGATTTAT TTGTAAATGC AAAATCGGGC AGACTACGTA TGGTACTGGT 
TTTGGCGCTA ACAAAAAGGA GGCAAAGCAG TTGGCTGCCA AAAATGCGTA TCAGAAGCTG 
TCAGAGAAAA GCCCATCGAA AACTGGCTTT GTCACATCTT TATCCAGTGA CTTTTCCAGC 
AGCTCGTCTA TAACAAGTAA CTCTGCTTCT CAGTCAGCAT CTGGAAGGGA TTTCGAGGAC 
ATATTTATGA ATGGTCTCAG AGAAAAAAGG AAATCAGGAG TAAAAGTGCC ATCTGATGAC 
GTGCTAAGAA ATAAATATAC CTTGGACGAC AGGTTTAGCA AAGATTTTGA AGACATAGAA 
GAAATTGGCT CGGGTGGATT TGGCCAAGTT TTCAAAGCAA AACACAGAAT CGATGGAAAG 
ACGTATGCTA TTAAGCGCAT TACATATAAC ACCAAGAAGG CAAAGCGTGA AGTACAAGCA 
CTAGCAGAAC TCAATCACGC CAACATTGTT CAATACCGAG TTTGTTGGGA GGGAGAGGAC 
TATGACTATG ATCCCGAGAA CAGCACAAAC GGTGACACAA GTCGATACAA GACCCGGTGC 
CTCTTTATCC AAATGGAATT CTGTGATAAA GGAACTCTGC AGCAGTGGTT GGAAAAGAGA 
AATCGGAGTC AAGAGGACAA GGCTTTGGTT TTGGAGTTAT TTGAACAAAT AGTGACAGGA 
GTGGATTATA TACACTCGAA AGGTTTAATT CATAGAGACC TTAAGCCAGG TAATATATTT 
TTAGTGGATG AAAAACACAT TAAGATCGGA GACTTTGGCC TTGCAACAGC CCTGGAAAAT 
GATGGAAATC CTCGAACAAA GTATACAGGA ACTCCTCAAT ACATGAGTCC AGAACAAAAG 
TCATCGTTAG TGGAATATGG AAAGGAAGTG GACATCTTTG CTTTGGGCCT TATTCTAGCT 
GAACTTCTTC ACATATGCAA AACTGATTCA GAGAAAATAG AGTTTTTCCA ACTTCTAAGA 
AATGGCGTCT TCTCCGATGA TATTTTCGAC AACAAGGAAA AAAGTCTTCT ACAGAAATTA 
CTCTCAAGTA AACCCAGGGA ACGACCCAAT ACGTCTGAAA TCCTGAAGAC TTTGGCTGAG 
TGGAAGAACA TCTCGGAGAA AAAGAAAAGA AACACGTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PKR regulates LPS-induced osteoclast formation and bone destruction in vitro and in vivo.
Oral diseases23(2)181-188(2017 Mar)
Teramachi J,Inagaki Y,Shinohara H,Okamura H,Yang D,Ochiai K,Baba R,Morimoto H,Nagata T,Haneji T

PKR and HMGB1 expression and function in rheumatoid arthritis.
Genetics and molecular research : GMR14(4)17864-70(2015 Dec)
Wang WJ,Yin SJ,Rong RQ

PERK pathway is involved in oxygen-glucose-serum deprivation-induced NF-kB activation via ROS generation in spinal cord astrocytes.
Biochemical and biophysical research communications467(2)197-203(2015 Nov)
Liu J,Du L

Zinc regulates expression of IL-23 p19 mRNA via activation of eIF2?/ATF4 axis in HAPI cells.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine28(5)891-902(2015 Oct)
Doi T,Hara H,Kajita M,Kamiya T,Adachi T

p58IPK is an inhibitor of the eIF2? kinase GCN2 and its localization and expression underpin protein synthesis and ER processing capacity.
The Biochemical journal465(2)213-25(2015 Jan)
Roobol A,Roobol J,Bastide A,Knight JR,Willis AE,Smales CM

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Eif2ak2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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