EIF2AK2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF2AK2
Entrez Gene ID	347700
Full Name	eukaryotic translation initiation factor 2 alpha kinase 2
Synonyms	PKR,PRKR
General protein information	Preferred Names eukaryotic translation initiation factor 2 alpha kinase 2 Names interferon-induced, double-stranded RNA-activated protein kinase protein kinase, interferon-inducible double stranded RNA dependent
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005212569.4	XP_005212626.1	interferon-induced, double-stranded RNA-activated protein kinase isoform X1
XM_005212569.3	XP_005212626.1	interferon-induced, double-stranded RNA-activated protein kinase isoform X1
NM_178109.3	NP_835210.2	interferon-induced, double-stranded RNA-activated protein kinase
XM_005212570.3	XP_005212627.1	interferon-induced, double-stranded RNA-activated protein kinase isoform X2
XM_005212570.4	XP_005212627.1	interferon-induced, double-stranded RNA-activated protein kinase isoform X2

Pathways from BioSystems

Homologies

	EIF2AK2	NP_001077417.1
Canis lupus familiaris (dog)	EIF2AK2	NP_001041600.1
Homo sapiens (human)	EIF2AK2	NP_002750.1
Pan troglodytes (chimpanzee)	EIF2AK2	NP_001138509.1
Gallus gallus (chicken)	EIF2AK2	NP_989818.1
Xenopus tropicalis (tropical clawed frog)	pkr3	XP_002933926.1
Bos taurus (cattle)	EIF2AK2	NP_835210.2
Rattus norvegicus (Norway rat)	Eif2ak2	NP_062208.1
Xenopus tropicalis (tropical clawed frog)	eif2ak2	NP_001072537.1
Mus musculus (house mouse)	Eif2ak2	NP_035293.1
Danio rerio (zebrafish)	eif2ak2	NP_001107942.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Modulation of PKR activity in cells infected by bovine viral diarrhea virus.
Gil LH, van Olphen AL, Mittal SK, Donis RO
Virus research116(1-2)69-77(2006 Mar)

The mRNA regulation of porcine double-stranded RNA-activated protein kinase gene.
Asano A, Kon Y, Agui T
The Journal of veterinary medical science66(12)1523-8(2004 Dec)

Tumour necrosis factor alpha up-regulates protein kinase R (PKR)-activating protein (PACT) and increases phosphorylation of PKR and eukaryotic initiation factor 2-alpha in articular chondrocytes.
Gilbert SJ, Duance VC, Mason DJ
Biochemical Society transactions30(Pt 6)886-9(2002 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Infection with cp BVDV was found to induce PKR activation.
Title: Modulation of PKR activity in cells infected by bovine viral diarrhea virus.

The following EIF2AK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF2AK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa23879	NM_178109.3 Latest version!	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa23879	XM_005212569.4 Latest version!	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa23880	XM_005212570.4 Latest version!	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
OBa23879	XM_005212569.3	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa23880	XM_005212570.3	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF2AK2 ( NM_178109.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_835210.2 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), mRNA.

Price & Availability↑

CloneID	OBa23879
Clone ID Related Accession (Same CDS sequence)	XM_005212569.4 , XM_005212569.3 , NM_178109.3
Accession Version	NM_178109.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-21
Organism	Bos taurus(cattle)
Product	interferon-induced, double-stranded RNA-activated protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC126646.1. On Aug 30, 2012 this sequence version replaced NM_178109.2. ##Evidence-Data-START## Transcript exon combination :: BC126646.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCACTG GTCGTTCACC ATGTTTCTAC ATAGAAGAAC TTAATAAATA CCAACAAAAG 
AATGGTGTAG AAGTTAGGTA TTGTGAACTG GCTAAGACAG GACCTCCACA TAATTTTAGG 
TTTACATATC AAGTTATAAT AGATGATAAA GAATATCCAA AGGCTGAAGG TAGATCAAAG 
AAGGAAGCCA AAAACGCTGC AGCCAAATTA GCTTTGGAAA TAATTAATAA AGACCGTAAG 
TCAGTTAGTT TTTCATCTCA GCTGACAACA AATATTCCAG AAGGACCACC CATTGAGAAT 
TACATAGGCC GTCTTAATAC GATCTCCCAG AAGAAAAACC TATGTGTAAC TTATGAAGAA 
TGTAAATCAA AGGGTGATGG GCCCGAAGGA TTTCATTATA TATGCAAAAT TGGACAAGAA 
GAATATGGTA GTGGTGTGGG TTCCACTAAA CAGGAGGCAA AACAGTTGGC TGCCAAACTG 
GCTTATGAAA AGATAGAATC AGAAACAATG AGAGATGGTT TCAGTGCTGC GTGTGGTGAT 
GTCGAAAGAA ACTCTCCAGC AGTGAGTACA TCTAGTTCTG AATCACCGTC TGAAAATGGC 
TTCTCAACAA ATGCATCAGA AAGAAGTGAT AACAACAGTG GCACATTAAA CAGCTCTTCC 
GTGTCTCCAG GGGACGGTTC CAGAAATAAT TCCAGGAAGG TGAAGAGAAG TTTGGCGCCT 
ACATTTGTCT CTCCTGTGAA GACAGAAGGA AACATTTATA GTGTGAACGA AAGGTTATTC 
AACGATTTCA CAGAAGTAAC ACCAATTGGC TCAGGTGGAT TTGGCCAAGT TTTCAAAGCC 
AAACACAGAA TTGATAAGAA GACTTATGTT ATTAAATGTG TTAAATATAA TAGTGAGAAG 
GTGGAGCGTG AAGTGAAAGC TTTGGCAACA CTTAATCATC CAAATATTGT TCATTACCAC 
AATTGTTGGG ATGGGCATGA TTATGATCCT GAGCAAAGCT TAAATTCTTC AAGATCAAAG 
ACTAGGTGCC TTTTCATCCA GATGGAATAT TGTGATAAAG GTACGTTGGA CCAATGGATT 
GAGAAAAGAA GAGGCAAGAA GCCAGACAAA CGTTTGGCTT TGGATTTCTT TCAACAAATA 
ACAACAGGAG TGCATTATAT ACATTCAGAA CAGTTAATTC ATAGAGACCT TAAGCCAGGT 
AACATATTTT TAGTAGCTAT GAACCAAATC AAGATTGGAG ACTTTGGACT TGTTACATAC 
CTGAAAAATG ATGAAACGCG GACAAGTAAA AAGGGAACTT TACGATACAT GAGCCCAGAA 
CAGCTTTCTT CTGTGAAAGA CTATGGAAAT GAAGTGGACA TCTATGCTTT GGGGCTGATT 
CTTGCAGAAC TTCTTCATAT ATGTCTCACT TCTTTAGAAA CACAGAAGTT CTTTGACGAT 
CTAAGGAATG GCAGATTAGA TGTATTTGAC GACAAAGAAA AAGATCTTCT GGAGAAATTA 
CTCTCAGTGG ACCCCAAGAA ACGACCTACC GCATCTGAGA TACTGAAGAC TTTGAAGGAG 
TGGAATAATG TTACAGAAAA AAAGAAACGA AACACATGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_835210.2
CDS	287..1888
Translation	MATGRSPCFYIEELNKYQQKNGVEVRYCELAKTGPPHNFRFTYQVIIDDKEYPKAEGRSKKEAKNAAAKLALEIINKDRKSVSFSSQLTTNIPEGPPIENYIGRLNTISQKKNLCVTYEECKSKGDGPEGFHYICKIGQEEYGSGVGSTKQEAKQLAAKLAYEKIESETMRDGFSAACGDVERNSPAVSTSSSESPSENGFSTNASERSDNNSGTLNSSSVSPGDGSRNNSRKVKRSLAPTFVSPVKTEGNIYSVNERLFNDFTEVTPIGSGGFGQVFKAKHRIDKKTYVIKCVKYNSEKVEREVKALATLNHPNIVHYHNCWDGHDYDPEQSLNSSRSKTRCLFIQMEYCDKGTLDQWIEKRRGKKPDKRLALDFFQQITTGVHYIHSEQLIHRDLKPGNIFLVAMNQIKIGDFGLVTYLKNDETRTSKKGTLRYMSPEQLSSVKDYGNEVDIYALGLILAELLHICLTSLETQKFFDDLRNGRLDVFDDKEKDLLEKLLSVDPKKRPTASEILKTLKEWNNVTEKKKRNTC

Target ORF information:

RefSeq Version	NM_178109.3
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178109.3

ORF Insert Sequence:

ATGGCCACTG GTCGTTCACC ATGTTTCTAC ATAGAAGAAC TTAATAAATA CCAACAAAAG 
AATGGTGTAG AAGTTAGGTA TTGTGAACTG GCTAAGACAG GACCTCCACA TAATTTTAGG 
TTTACATATC AAGTTATAAT AGATGATAAA GAATATCCAA AGGCTGAAGG TAGATCAAAG 
AAGGAAGCCA AAAACGCTGC AGCCAAATTA GCTTTGGAAA TAATTAATAA AGACCGTAAG 
TCAGTTAGTT TTTCATCTCA GCTGACAACA AATATTCCAG AAGGACCACC CATTGAGAAT 
TACATAGGCC GTCTTAATAC GATCTCCCAG AAGAAAAACC TATGTGTAAC TTATGAAGAA 
TGTAAATCAA AGGGTGATGG GCCCGAAGGA TTTCATTATA TATGCAAAAT TGGACAAGAA 
GAATATGGTA GTGGTGTGGG TTCCACTAAA CAGGAGGCAA AACAGTTGGC TGCCAAACTG 
GCTTATGAAA AGATAGAATC AGAAACAATG AGAGATGGTT TCAGTGCTGC GTGTGGTGAT 
GTCGAAAGAA ACTCTCCAGC AGTGAGTACA TCTAGTTCTG AATCACCGTC TGAAAATGGC 
TTCTCAACAA ATGCATCAGA AAGAAGTGAT AACAACAGTG GCACATTAAA CAGCTCTTCC 
GTGTCTCCAG GGGACGGTTC CAGAAATAAT TCCAGGAAGG TGAAGAGAAG TTTGGCGCCT 
ACATTTGTCT CTCCTGTGAA GACAGAAGGA AACATTTATA GTGTGAACGA AAGGTTATTC 
AACGATTTCA CAGAAGTAAC ACCAATTGGC TCAGGTGGAT TTGGCCAAGT TTTCAAAGCC 
AAACACAGAA TTGATAAGAA GACTTATGTT ATTAAATGTG TTAAATATAA TAGTGAGAAG 
GTGGAGCGTG AAGTGAAAGC TTTGGCAACA CTTAATCATC CAAATATTGT TCATTACCAC 
AATTGTTGGG ATGGGCATGA TTATGATCCT GAGCAAAGCT TAAATTCTTC AAGATCAAAG 
ACTAGGTGCC TTTTCATCCA GATGGAATAT TGTGATAAAG GTACGTTGGA CCAATGGATT 
GAGAAAAGAA GAGGCAAGAA GCCAGACAAA CGTTTGGCTT TGGATTTCTT TCAACAAATA 
ACAACAGGAG TGCATTATAT ACATTCAGAA CAGTTAATTC ATAGAGACCT TAAGCCAGGT 
AACATATTTT TAGTAGCTAT GAACCAAATC AAGATTGGAG ACTTTGGACT TGTTACATAC 
CTGAAAAATG ATGAAACGCG GACAAGTAAA AAGGGAACTT TACGATACAT GAGCCCAGAA 
CAGCTTTCTT CTGTGAAAGA CTATGGAAAT GAAGTGGACA TCTATGCTTT GGGGCTGATT 
CTTGCAGAAC TTCTTCATAT ATGTCTCACT TCTTTAGAAA CACAGAAGTT CTTTGACGAT 
CTAAGGAATG GCAGATTAGA TGTATTTGAC GACAAAGAAA AAGATCTTCT GGAGAAATTA 
CTCTCAGTGG ACCCCAAGAA ACGACCTACC GCATCTGAGA TACTGAAGAC TTTGAAGGAG 
TGGAATAATG TTACAGAAAA AAAGAAACGA AACACATGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK2 ( XM_005212569.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212626.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23879
Clone ID Related Accession (Same CDS sequence)	XM_005212569.4 , XM_005212569.3 , NM_178109.3
Accession Version	XM_005212569.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	interferon-induced, double-stranded RNA-activated protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212569.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG GTCGTTCACC ATGTTTCTAC ATAGAAGAAC TTAATAAATA CCAACAAAAG 
AATGGTGTAG AAGTTAGGTA TTGTGAACTG GCTAAGACAG GACCTCCACA TAATTTTAGG 
TTTACATATC AAGTTATAAT AGATGATAAA GAATATCCAA AGGCTGAAGG TAGATCAAAG 
AAGGAAGCCA AAAACGCTGC AGCCAAATTA GCTTTGGAAA TAATTAATAA AGACCGTAAG 
TCAGTTAGTT TTTCATCTCA GCTGACAACA AATATTCCAG AAGGACCACC CATTGAGAAT 
TACATAGGCC GTCTTAATAC GATCTCCCAG AAGAAAAACC TATGTGTAAC TTATGAAGAA 
TGTAAATCAA AGGGTGATGG GCCCGAAGGA TTTCATTATA TATGCAAAAT TGGACAAGAA 
GAATATGGTA GTGGTGTGGG TTCCACTAAA CAGGAGGCAA AACAGTTGGC TGCCAAACTG 
GCTTATGAAA AGATAGAATC AGAAACAATG AGAGATGGTT TCAGTGCTGC GTGTGGTGAT 
GTCGAAAGAA ACTCTCCAGC AGTGAGTACA TCTAGTTCTG AATCACCGTC TGAAAATGGC 
TTCTCAACAA ATGCATCAGA AAGAAGTGAT AACAACAGTG GCACATTAAA CAGCTCTTCC 
GTGTCTCCAG GGGACGGTTC CAGAAATAAT TCCAGGAAGG TGAAGAGAAG TTTGGCGCCT 
ACATTTGTCT CTCCTGTGAA GACAGAAGGA AACATTTATA GTGTGAACGA AAGGTTATTC 
AACGATTTCA CAGAAGTAAC ACCAATTGGC TCAGGTGGAT TTGGCCAAGT TTTCAAAGCC 
AAACACAGAA TTGATAAGAA GACTTATGTT ATTAAATGTG TTAAATATAA TAGTGAGAAG 
GTGGAGCGTG AAGTGAAAGC TTTGGCAACA CTTAATCATC CAAATATTGT TCATTACCAC 
AATTGTTGGG ATGGGCATGA TTATGATCCT GAGCAAAGCT TAAATTCTTC AAGATCAAAG 
ACTAGGTGCC TTTTCATCCA GATGGAATAT TGTGATAAAG GTACGTTGGA CCAATGGATT 
GAGAAAAGAA GAGGCAAGAA GCCAGACAAA CGTTTGGCTT TGGATTTCTT TCAACAAATA 
ACAACAGGAG TGCATTATAT ACATTCAGAA CAGTTAATTC ATAGAGACCT TAAGCCAGGT 
AACATATTTT TAGTAGCTAT GAACCAAATC AAGATTGGAG ACTTTGGACT TGTTACATAC 
CTGAAAAATG ATGAAACGCG GACAAGTAAA AAGGGAACTT TACGATACAT GAGCCCAGAA 
CAGCTTTCTT CTGTGAAAGA CTATGGAAAT GAAGTGGACA TCTATGCTTT GGGGCTGATT 
CTTGCAGAAC TTCTTCATAT ATGTCTCACT TCTTTAGAAA CACAGAAGTT CTTTGACGAT 
CTAAGGAATG GCAGATTAGA TGTATTTGAC GACAAAGAAA AAGATCTTCT GGAGAAATTA 
CTCTCAGTGG ACCCCAAGAA ACGACCTACC GCATCTGAGA TACTGAAGAC TTTGAAGGAG 
TGGAATAATG TTACAGAAAA AAAGAAACGA AACACATGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212626.1
CDS	282..1883
Translation	MATGRSPCFYIEELNKYQQKNGVEVRYCELAKTGPPHNFRFTYQVIIDDKEYPKAEGRSKKEAKNAAAKLALEIINKDRKSVSFSSQLTTNIPEGPPIENYIGRLNTISQKKNLCVTYEECKSKGDGPEGFHYICKIGQEEYGSGVGSTKQEAKQLAAKLAYEKIESETMRDGFSAACGDVERNSPAVSTSSSESPSENGFSTNASERSDNNSGTLNSSSVSPGDGSRNNSRKVKRSLAPTFVSPVKTEGNIYSVNERLFNDFTEVTPIGSGGFGQVFKAKHRIDKKTYVIKCVKYNSEKVEREVKALATLNHPNIVHYHNCWDGHDYDPEQSLNSSRSKTRCLFIQMEYCDKGTLDQWIEKRRGKKPDKRLALDFFQQITTGVHYIHSEQLIHRDLKPGNIFLVAMNQIKIGDFGLVTYLKNDETRTSKKGTLRYMSPEQLSSVKDYGNEVDIYALGLILAELLHICLTSLETQKFFDDLRNGRLDVFDDKEKDLLEKLLSVDPKKRPTASEILKTLKEWNNVTEKKKRNTC

Target ORF information:

RefSeq Version	XM_005212569.4
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212569.4

ORF Insert Sequence:

ATGGCCACTG GTCGTTCACC ATGTTTCTAC ATAGAAGAAC TTAATAAATA CCAACAAAAG 
AATGGTGTAG AAGTTAGGTA TTGTGAACTG GCTAAGACAG GACCTCCACA TAATTTTAGG 
TTTACATATC AAGTTATAAT AGATGATAAA GAATATCCAA AGGCTGAAGG TAGATCAAAG 
AAGGAAGCCA AAAACGCTGC AGCCAAATTA GCTTTGGAAA TAATTAATAA AGACCGTAAG 
TCAGTTAGTT TTTCATCTCA GCTGACAACA AATATTCCAG AAGGACCACC CATTGAGAAT 
TACATAGGCC GTCTTAATAC GATCTCCCAG AAGAAAAACC TATGTGTAAC TTATGAAGAA 
TGTAAATCAA AGGGTGATGG GCCCGAAGGA TTTCATTATA TATGCAAAAT TGGACAAGAA 
GAATATGGTA GTGGTGTGGG TTCCACTAAA CAGGAGGCAA AACAGTTGGC TGCCAAACTG 
GCTTATGAAA AGATAGAATC AGAAACAATG AGAGATGGTT TCAGTGCTGC GTGTGGTGAT 
GTCGAAAGAA ACTCTCCAGC AGTGAGTACA TCTAGTTCTG AATCACCGTC TGAAAATGGC 
TTCTCAACAA ATGCATCAGA AAGAAGTGAT AACAACAGTG GCACATTAAA CAGCTCTTCC 
GTGTCTCCAG GGGACGGTTC CAGAAATAAT TCCAGGAAGG TGAAGAGAAG TTTGGCGCCT 
ACATTTGTCT CTCCTGTGAA GACAGAAGGA AACATTTATA GTGTGAACGA AAGGTTATTC 
AACGATTTCA CAGAAGTAAC ACCAATTGGC TCAGGTGGAT TTGGCCAAGT TTTCAAAGCC 
AAACACAGAA TTGATAAGAA GACTTATGTT ATTAAATGTG TTAAATATAA TAGTGAGAAG 
GTGGAGCGTG AAGTGAAAGC TTTGGCAACA CTTAATCATC CAAATATTGT TCATTACCAC 
AATTGTTGGG ATGGGCATGA TTATGATCCT GAGCAAAGCT TAAATTCTTC AAGATCAAAG 
ACTAGGTGCC TTTTCATCCA GATGGAATAT TGTGATAAAG GTACGTTGGA CCAATGGATT 
GAGAAAAGAA GAGGCAAGAA GCCAGACAAA CGTTTGGCTT TGGATTTCTT TCAACAAATA 
ACAACAGGAG TGCATTATAT ACATTCAGAA CAGTTAATTC ATAGAGACCT TAAGCCAGGT 
AACATATTTT TAGTAGCTAT GAACCAAATC AAGATTGGAG ACTTTGGACT TGTTACATAC 
CTGAAAAATG ATGAAACGCG GACAAGTAAA AAGGGAACTT TACGATACAT GAGCCCAGAA 
CAGCTTTCTT CTGTGAAAGA CTATGGAAAT GAAGTGGACA TCTATGCTTT GGGGCTGATT 
CTTGCAGAAC TTCTTCATAT ATGTCTCACT TCTTTAGAAA CACAGAAGTT CTTTGACGAT 
CTAAGGAATG GCAGATTAGA TGTATTTGAC GACAAAGAAA AAGATCTTCT GGAGAAATTA 
CTCTCAGTGG ACCCCAAGAA ACGACCTACC GCATCTGAGA TACTGAAGAC TTTGAAGGAG 
TGGAATAATG TTACAGAAAA AAAGAAACGA AACACATGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK2 ( XM_005212569.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212626.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23879
Clone ID Related Accession (Same CDS sequence)	XM_005212569.4 , XM_005212569.3 , NM_178109.3
Accession Version	XM_005212569.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	interferon-induced, double-stranded RNA-activated protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212569.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG GTCGTTCACC ATGTTTCTAC ATAGAAGAAC TTAATAAATA CCAACAAAAG 
AATGGTGTAG AAGTTAGGTA TTGTGAACTG GCTAAGACAG GACCTCCACA TAATTTTAGG 
TTTACATATC AAGTTATAAT AGATGATAAA GAATATCCAA AGGCTGAAGG TAGATCAAAG 
AAGGAAGCCA AAAACGCTGC AGCCAAATTA GCTTTGGAAA TAATTAATAA AGACCGTAAG 
TCAGTTAGTT TTTCATCTCA GCTGACAACA AATATTCCAG AAGGACCACC CATTGAGAAT 
TACATAGGCC GTCTTAATAC GATCTCCCAG AAGAAAAACC TATGTGTAAC TTATGAAGAA 
TGTAAATCAA AGGGTGATGG GCCCGAAGGA TTTCATTATA TATGCAAAAT TGGACAAGAA 
GAATATGGTA GTGGTGTGGG TTCCACTAAA CAGGAGGCAA AACAGTTGGC TGCCAAACTG 
GCTTATGAAA AGATAGAATC AGAAACAATG AGAGATGGTT TCAGTGCTGC GTGTGGTGAT 
GTCGAAAGAA ACTCTCCAGC AGTGAGTACA TCTAGTTCTG AATCACCGTC TGAAAATGGC 
TTCTCAACAA ATGCATCAGA AAGAAGTGAT AACAACAGTG GCACATTAAA CAGCTCTTCC 
GTGTCTCCAG GGGACGGTTC CAGAAATAAT TCCAGGAAGG TGAAGAGAAG TTTGGCGCCT 
ACATTTGTCT CTCCTGTGAA GACAGAAGGA AACATTTATA GTGTGAACGA AAGGTTATTC 
AACGATTTCA CAGAAGTAAC ACCAATTGGC TCAGGTGGAT TTGGCCAAGT TTTCAAAGCC 
AAACACAGAA TTGATAAGAA GACTTATGTT ATTAAATGTG TTAAATATAA TAGTGAGAAG 
GTGGAGCGTG AAGTGAAAGC TTTGGCAACA CTTAATCATC CAAATATTGT TCATTACCAC 
AATTGTTGGG ATGGGCATGA TTATGATCCT GAGCAAAGCT TAAATTCTTC AAGATCAAAG 
ACTAGGTGCC TTTTCATCCA GATGGAATAT TGTGATAAAG GTACGTTGGA CCAATGGATT 
GAGAAAAGAA GAGGCAAGAA GCCAGACAAA CGTTTGGCTT TGGATTTCTT TCAACAAATA 
ACAACAGGAG TGCATTATAT ACATTCAGAA CAGTTAATTC ATAGAGACCT TAAGCCAGGT 
AACATATTTT TAGTAGCTAT GAACCAAATC AAGATTGGAG ACTTTGGACT TGTTACATAC 
CTGAAAAATG ATGAAACGCG GACAAGTAAA AAGGGAACTT TACGATACAT GAGCCCAGAA 
CAGCTTTCTT CTGTGAAAGA CTATGGAAAT GAAGTGGACA TCTATGCTTT GGGGCTGATT 
CTTGCAGAAC TTCTTCATAT ATGTCTCACT TCTTTAGAAA CACAGAAGTT CTTTGACGAT 
CTAAGGAATG GCAGATTAGA TGTATTTGAC GACAAAGAAA AAGATCTTCT GGAGAAATTA 
CTCTCAGTGG ACCCCAAGAA ACGACCTACC GCATCTGAGA TACTGAAGAC TTTGAAGGAG 
TGGAATAATG TTACAGAAAA AAAGAAACGA AACACATGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212626.1
CDS	282..1883
Translation	MATGRSPCFYIEELNKYQQKNGVEVRYCELAKTGPPHNFRFTYQVIIDDKEYPKAEGRSKKEAKNAAAKLALEIINKDRKSVSFSSQLTTNIPEGPPIENYIGRLNTISQKKNLCVTYEECKSKGDGPEGFHYICKIGQEEYGSGVGSTKQEAKQLAAKLAYEKIESETMRDGFSAACGDVERNSPAVSTSSSESPSENGFSTNASERSDNNSGTLNSSSVSPGDGSRNNSRKVKRSLAPTFVSPVKTEGNIYSVNERLFNDFTEVTPIGSGGFGQVFKAKHRIDKKTYVIKCVKYNSEKVEREVKALATLNHPNIVHYHNCWDGHDYDPEQSLNSSRSKTRCLFIQMEYCDKGTLDQWIEKRRGKKPDKRLALDFFQQITTGVHYIHSEQLIHRDLKPGNIFLVAMNQIKIGDFGLVTYLKNDETRTSKKGTLRYMSPEQLSSVKDYGNEVDIYALGLILAELLHICLTSLETQKFFDDLRNGRLDVFDDKEKDLLEKLLSVDPKKRPTASEILKTLKEWNNVTEKKKRNTC

Target ORF information:

RefSeq Version	XM_005212569.3
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212569.3

ORF Insert Sequence:

ATGGCCACTG GTCGTTCACC ATGTTTCTAC ATAGAAGAAC TTAATAAATA CCAACAAAAG 
AATGGTGTAG AAGTTAGGTA TTGTGAACTG GCTAAGACAG GACCTCCACA TAATTTTAGG 
TTTACATATC AAGTTATAAT AGATGATAAA GAATATCCAA AGGCTGAAGG TAGATCAAAG 
AAGGAAGCCA AAAACGCTGC AGCCAAATTA GCTTTGGAAA TAATTAATAA AGACCGTAAG 
TCAGTTAGTT TTTCATCTCA GCTGACAACA AATATTCCAG AAGGACCACC CATTGAGAAT 
TACATAGGCC GTCTTAATAC GATCTCCCAG AAGAAAAACC TATGTGTAAC TTATGAAGAA 
TGTAAATCAA AGGGTGATGG GCCCGAAGGA TTTCATTATA TATGCAAAAT TGGACAAGAA 
GAATATGGTA GTGGTGTGGG TTCCACTAAA CAGGAGGCAA AACAGTTGGC TGCCAAACTG 
GCTTATGAAA AGATAGAATC AGAAACAATG AGAGATGGTT TCAGTGCTGC GTGTGGTGAT 
GTCGAAAGAA ACTCTCCAGC AGTGAGTACA TCTAGTTCTG AATCACCGTC TGAAAATGGC 
TTCTCAACAA ATGCATCAGA AAGAAGTGAT AACAACAGTG GCACATTAAA CAGCTCTTCC 
GTGTCTCCAG GGGACGGTTC CAGAAATAAT TCCAGGAAGG TGAAGAGAAG TTTGGCGCCT 
ACATTTGTCT CTCCTGTGAA GACAGAAGGA AACATTTATA GTGTGAACGA AAGGTTATTC 
AACGATTTCA CAGAAGTAAC ACCAATTGGC TCAGGTGGAT TTGGCCAAGT TTTCAAAGCC 
AAACACAGAA TTGATAAGAA GACTTATGTT ATTAAATGTG TTAAATATAA TAGTGAGAAG 
GTGGAGCGTG AAGTGAAAGC TTTGGCAACA CTTAATCATC CAAATATTGT TCATTACCAC 
AATTGTTGGG ATGGGCATGA TTATGATCCT GAGCAAAGCT TAAATTCTTC AAGATCAAAG 
ACTAGGTGCC TTTTCATCCA GATGGAATAT TGTGATAAAG GTACGTTGGA CCAATGGATT 
GAGAAAAGAA GAGGCAAGAA GCCAGACAAA CGTTTGGCTT TGGATTTCTT TCAACAAATA 
ACAACAGGAG TGCATTATAT ACATTCAGAA CAGTTAATTC ATAGAGACCT TAAGCCAGGT 
AACATATTTT TAGTAGCTAT GAACCAAATC AAGATTGGAG ACTTTGGACT TGTTACATAC 
CTGAAAAATG ATGAAACGCG GACAAGTAAA AAGGGAACTT TACGATACAT GAGCCCAGAA 
CAGCTTTCTT CTGTGAAAGA CTATGGAAAT GAAGTGGACA TCTATGCTTT GGGGCTGATT 
CTTGCAGAAC TTCTTCATAT ATGTCTCACT TCTTTAGAAA CACAGAAGTT CTTTGACGAT 
CTAAGGAATG GCAGATTAGA TGTATTTGAC GACAAAGAAA AAGATCTTCT GGAGAAATTA 
CTCTCAGTGG ACCCCAAGAA ACGACCTACC GCATCTGAGA TACTGAAGAC TTTGAAGGAG 
TGGAATAATG TTACAGAAAA AAAGAAACGA AACACATGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK2 ( XM_005212570.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212627.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa23880
Clone ID Related Accession (Same CDS sequence)	XM_005212570.3 , XM_005212570.4
Accession Version	XM_005212570.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	interferon-induced, double-stranded RNA-activated protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212570.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG GTCGTTCACC ATGTTTCTAC ATAGAAGAAC TTAATAAATA CCAACAAAAG 
AATGGTGTAG AAGTTAGGTA TTGTGAACTG GCTAAGACAG GACCTCCACA TAATTTTAGG 
TTTACATATC AAGTTATAAT AGATGATAAA GAATATCCAA AGGCTGAAGG TAGATCAAAG 
AAGGAAGCCA AAAACGCTGC AGCCAAATTA GCTTTGGAAA TAATTAATAA AGACCGTAAG 
TCAGTTAGTT TTTCATCTCA GCTGACAACA AATATTCCAG AAGGACCACC CATTGAGAAT 
TACATAGGCC GTCTTAATAC GATCTCCCAG AAGAAAAACC TATGTGTAAC TTATGAAGAA 
TGTAAATCAA AGGGTGATGG GCCCGAAGGA TTTCATTATA TATGCAAAAT TGGACAAGAA 
GAATATGGTA GTGGTGTGGG TTCCACTAAA CAGGAGGCAA AACAGTTGGC TGCCAAACTG 
GCTTATGAAA AGATAGAATC AGAAACAATG AGAGATGGTT TCAGTGCTGC GTGTGGTGAT 
GTCGAAAGAA ACTCTCCAGC AGTGAGTACA TCTTCTGAAT CACCGTCTGA AAATGGCTTC 
TCAACAAATG CATCAGAAAG AAGTGATAAC AACAGTGGCA CATTAAACAG CTCTTCCGTG 
TCTCCAGGGG ACGGTTCCAG AAATAATTCC AGGAAGGTGA AGAGAAGTTT GGCGCCTACA 
TTTGTCTCTC CTGTGAAGAC AGAAGGAAAC ATTTATAGTG TGAACGAAAG GTTATTCAAC 
GATTTCACAG AAGTAACACC AATTGGCTCA GGTGGATTTG GCCAAGTTTT CAAAGCCAAA 
CACAGAATTG ATAAGAAGAC TTATGTTATT AAATGTGTTA AATATAATAG TGAGAAGGTG 
GAGCGTGAAG TGAAAGCTTT GGCAACACTT AATCATCCAA ATATTGTTCA TTACCACAAT 
TGTTGGGATG GGCATGATTA TGATCCTGAG CAAAGCTTAA ATTCTTCAAG ATCAAAGACT 
AGGTGCCTTT TCATCCAGAT GGAATATTGT GATAAAGGTA CGTTGGACCA ATGGATTGAG 
AAAAGAAGAG GCAAGAAGCC AGACAAACGT TTGGCTTTGG ATTTCTTTCA ACAAATAACA 
ACAGGAGTGC ATTATATACA TTCAGAACAG TTAATTCATA GAGACCTTAA GCCAGGTAAC 
ATATTTTTAG TAGCTATGAA CCAAATCAAG ATTGGAGACT TTGGACTTGT TACATACCTG 
AAAAATGATG AAACGCGGAC AAGTAAAAAG GGAACTTTAC GATACATGAG CCCAGAACAG 
CTTTCTTCTG TGAAAGACTA TGGAAATGAA GTGGACATCT ATGCTTTGGG GCTGATTCTT 
GCAGAACTTC TTCATATATG TCTCACTTCT TTAGAAACAC AGAAGTTCTT TGACGATCTA 
AGGAATGGCA GATTAGATGT ATTTGACGAC AAAGAAAAAG ATCTTCTGGA GAAATTACTC 
TCAGTGGACC CCAAGAAACG ACCTACCGCA TCTGAGATAC TGAAGACTTT GAAGGAGTGG 
AATAATGTTA CAGAAAAAAA GAAACGAAAC ACATGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212627.1
CDS	125..1723
Translation	MATGRSPCFYIEELNKYQQKNGVEVRYCELAKTGPPHNFRFTYQVIIDDKEYPKAEGRSKKEAKNAAAKLALEIINKDRKSVSFSSQLTTNIPEGPPIENYIGRLNTISQKKNLCVTYEECKSKGDGPEGFHYICKIGQEEYGSGVGSTKQEAKQLAAKLAYEKIESETMRDGFSAACGDVERNSPAVSTSSESPSENGFSTNASERSDNNSGTLNSSSVSPGDGSRNNSRKVKRSLAPTFVSPVKTEGNIYSVNERLFNDFTEVTPIGSGGFGQVFKAKHRIDKKTYVIKCVKYNSEKVEREVKALATLNHPNIVHYHNCWDGHDYDPEQSLNSSRSKTRCLFIQMEYCDKGTLDQWIEKRRGKKPDKRLALDFFQQITTGVHYIHSEQLIHRDLKPGNIFLVAMNQIKIGDFGLVTYLKNDETRTSKKGTLRYMSPEQLSSVKDYGNEVDIYALGLILAELLHICLTSLETQKFFDDLRNGRLDVFDDKEKDLLEKLLSVDPKKRPTASEILKTLKEWNNVTEKKKRNTC

Target ORF information:

RefSeq Version	XM_005212570.3
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212570.3

ORF Insert Sequence:

ATGGCCACTG GTCGTTCACC ATGTTTCTAC ATAGAAGAAC TTAATAAATA CCAACAAAAG 
AATGGTGTAG AAGTTAGGTA TTGTGAACTG GCTAAGACAG GACCTCCACA TAATTTTAGG 
TTTACATATC AAGTTATAAT AGATGATAAA GAATATCCAA AGGCTGAAGG TAGATCAAAG 
AAGGAAGCCA AAAACGCTGC AGCCAAATTA GCTTTGGAAA TAATTAATAA AGACCGTAAG 
TCAGTTAGTT TTTCATCTCA GCTGACAACA AATATTCCAG AAGGACCACC CATTGAGAAT 
TACATAGGCC GTCTTAATAC GATCTCCCAG AAGAAAAACC TATGTGTAAC TTATGAAGAA 
TGTAAATCAA AGGGTGATGG GCCCGAAGGA TTTCATTATA TATGCAAAAT TGGACAAGAA 
GAATATGGTA GTGGTGTGGG TTCCACTAAA CAGGAGGCAA AACAGTTGGC TGCCAAACTG 
GCTTATGAAA AGATAGAATC AGAAACAATG AGAGATGGTT TCAGTGCTGC GTGTGGTGAT 
GTCGAAAGAA ACTCTCCAGC AGTGAGTACA TCTTCTGAAT CACCGTCTGA AAATGGCTTC 
TCAACAAATG CATCAGAAAG AAGTGATAAC AACAGTGGCA CATTAAACAG CTCTTCCGTG 
TCTCCAGGGG ACGGTTCCAG AAATAATTCC AGGAAGGTGA AGAGAAGTTT GGCGCCTACA 
TTTGTCTCTC CTGTGAAGAC AGAAGGAAAC ATTTATAGTG TGAACGAAAG GTTATTCAAC 
GATTTCACAG AAGTAACACC AATTGGCTCA GGTGGATTTG GCCAAGTTTT CAAAGCCAAA 
CACAGAATTG ATAAGAAGAC TTATGTTATT AAATGTGTTA AATATAATAG TGAGAAGGTG 
GAGCGTGAAG TGAAAGCTTT GGCAACACTT AATCATCCAA ATATTGTTCA TTACCACAAT 
TGTTGGGATG GGCATGATTA TGATCCTGAG CAAAGCTTAA ATTCTTCAAG ATCAAAGACT 
AGGTGCCTTT TCATCCAGAT GGAATATTGT GATAAAGGTA CGTTGGACCA ATGGATTGAG 
AAAAGAAGAG GCAAGAAGCC AGACAAACGT TTGGCTTTGG ATTTCTTTCA ACAAATAACA 
ACAGGAGTGC ATTATATACA TTCAGAACAG TTAATTCATA GAGACCTTAA GCCAGGTAAC 
ATATTTTTAG TAGCTATGAA CCAAATCAAG ATTGGAGACT TTGGACTTGT TACATACCTG 
AAAAATGATG AAACGCGGAC AAGTAAAAAG GGAACTTTAC GATACATGAG CCCAGAACAG 
CTTTCTTCTG TGAAAGACTA TGGAAATGAA GTGGACATCT ATGCTTTGGG GCTGATTCTT 
GCAGAACTTC TTCATATATG TCTCACTTCT TTAGAAACAC AGAAGTTCTT TGACGATCTA 
AGGAATGGCA GATTAGATGT ATTTGACGAC AAAGAAAAAG ATCTTCTGGA GAAATTACTC 
TCAGTGGACC CCAAGAAACG ACCTACCGCA TCTGAGATAC TGAAGACTTT GAAGGAGTGG 
AATAATGTTA CAGAAAAAAA GAAACGAAAC ACATGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK2 ( XM_005212570.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212627.1 Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa23880
Clone ID Related Accession (Same CDS sequence)	XM_005212570.3 , XM_005212570.4
Accession Version	XM_005212570.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	interferon-induced, double-stranded RNA-activated protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212570.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG GTCGTTCACC ATGTTTCTAC ATAGAAGAAC TTAATAAATA CCAACAAAAG 
AATGGTGTAG AAGTTAGGTA TTGTGAACTG GCTAAGACAG GACCTCCACA TAATTTTAGG 
TTTACATATC AAGTTATAAT AGATGATAAA GAATATCCAA AGGCTGAAGG TAGATCAAAG 
AAGGAAGCCA AAAACGCTGC AGCCAAATTA GCTTTGGAAA TAATTAATAA AGACCGTAAG 
TCAGTTAGTT TTTCATCTCA GCTGACAACA AATATTCCAG AAGGACCACC CATTGAGAAT 
TACATAGGCC GTCTTAATAC GATCTCCCAG AAGAAAAACC TATGTGTAAC TTATGAAGAA 
TGTAAATCAA AGGGTGATGG GCCCGAAGGA TTTCATTATA TATGCAAAAT TGGACAAGAA 
GAATATGGTA GTGGTGTGGG TTCCACTAAA CAGGAGGCAA AACAGTTGGC TGCCAAACTG 
GCTTATGAAA AGATAGAATC AGAAACAATG AGAGATGGTT TCAGTGCTGC GTGTGGTGAT 
GTCGAAAGAA ACTCTCCAGC AGTGAGTACA TCTTCTGAAT CACCGTCTGA AAATGGCTTC 
TCAACAAATG CATCAGAAAG AAGTGATAAC AACAGTGGCA CATTAAACAG CTCTTCCGTG 
TCTCCAGGGG ACGGTTCCAG AAATAATTCC AGGAAGGTGA AGAGAAGTTT GGCGCCTACA 
TTTGTCTCTC CTGTGAAGAC AGAAGGAAAC ATTTATAGTG TGAACGAAAG GTTATTCAAC 
GATTTCACAG AAGTAACACC AATTGGCTCA GGTGGATTTG GCCAAGTTTT CAAAGCCAAA 
CACAGAATTG ATAAGAAGAC TTATGTTATT AAATGTGTTA AATATAATAG TGAGAAGGTG 
GAGCGTGAAG TGAAAGCTTT GGCAACACTT AATCATCCAA ATATTGTTCA TTACCACAAT 
TGTTGGGATG GGCATGATTA TGATCCTGAG CAAAGCTTAA ATTCTTCAAG ATCAAAGACT 
AGGTGCCTTT TCATCCAGAT GGAATATTGT GATAAAGGTA CGTTGGACCA ATGGATTGAG 
AAAAGAAGAG GCAAGAAGCC AGACAAACGT TTGGCTTTGG ATTTCTTTCA ACAAATAACA 
ACAGGAGTGC ATTATATACA TTCAGAACAG TTAATTCATA GAGACCTTAA GCCAGGTAAC 
ATATTTTTAG TAGCTATGAA CCAAATCAAG ATTGGAGACT TTGGACTTGT TACATACCTG 
AAAAATGATG AAACGCGGAC AAGTAAAAAG GGAACTTTAC GATACATGAG CCCAGAACAG 
CTTTCTTCTG TGAAAGACTA TGGAAATGAA GTGGACATCT ATGCTTTGGG GCTGATTCTT 
GCAGAACTTC TTCATATATG TCTCACTTCT TTAGAAACAC AGAAGTTCTT TGACGATCTA 
AGGAATGGCA GATTAGATGT ATTTGACGAC AAAGAAAAAG ATCTTCTGGA GAAATTACTC 
TCAGTGGACC CCAAGAAACG ACCTACCGCA TCTGAGATAC TGAAGACTTT GAAGGAGTGG 
AATAATGTTA CAGAAAAAAA GAAACGAAAC ACATGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212627.1
CDS	282..1880
Translation	MATGRSPCFYIEELNKYQQKNGVEVRYCELAKTGPPHNFRFTYQVIIDDKEYPKAEGRSKKEAKNAAAKLALEIINKDRKSVSFSSQLTTNIPEGPPIENYIGRLNTISQKKNLCVTYEECKSKGDGPEGFHYICKIGQEEYGSGVGSTKQEAKQLAAKLAYEKIESETMRDGFSAACGDVERNSPAVSTSSESPSENGFSTNASERSDNNSGTLNSSSVSPGDGSRNNSRKVKRSLAPTFVSPVKTEGNIYSVNERLFNDFTEVTPIGSGGFGQVFKAKHRIDKKTYVIKCVKYNSEKVEREVKALATLNHPNIVHYHNCWDGHDYDPEQSLNSSRSKTRCLFIQMEYCDKGTLDQWIEKRRGKKPDKRLALDFFQQITTGVHYIHSEQLIHRDLKPGNIFLVAMNQIKIGDFGLVTYLKNDETRTSKKGTLRYMSPEQLSSVKDYGNEVDIYALGLILAELLHICLTSLETQKFFDDLRNGRLDVFDDKEKDLLEKLLSVDPKKRPTASEILKTLKEWNNVTEKKKRNTC

Target ORF information:

RefSeq Version	XM_005212570.4
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212570.4

ORF Insert Sequence:

ATGGCCACTG GTCGTTCACC ATGTTTCTAC ATAGAAGAAC TTAATAAATA CCAACAAAAG 
AATGGTGTAG AAGTTAGGTA TTGTGAACTG GCTAAGACAG GACCTCCACA TAATTTTAGG 
TTTACATATC AAGTTATAAT AGATGATAAA GAATATCCAA AGGCTGAAGG TAGATCAAAG 
AAGGAAGCCA AAAACGCTGC AGCCAAATTA GCTTTGGAAA TAATTAATAA AGACCGTAAG 
TCAGTTAGTT TTTCATCTCA GCTGACAACA AATATTCCAG AAGGACCACC CATTGAGAAT 
TACATAGGCC GTCTTAATAC GATCTCCCAG AAGAAAAACC TATGTGTAAC TTATGAAGAA 
TGTAAATCAA AGGGTGATGG GCCCGAAGGA TTTCATTATA TATGCAAAAT TGGACAAGAA 
GAATATGGTA GTGGTGTGGG TTCCACTAAA CAGGAGGCAA AACAGTTGGC TGCCAAACTG 
GCTTATGAAA AGATAGAATC AGAAACAATG AGAGATGGTT TCAGTGCTGC GTGTGGTGAT 
GTCGAAAGAA ACTCTCCAGC AGTGAGTACA TCTTCTGAAT CACCGTCTGA AAATGGCTTC 
TCAACAAATG CATCAGAAAG AAGTGATAAC AACAGTGGCA CATTAAACAG CTCTTCCGTG 
TCTCCAGGGG ACGGTTCCAG AAATAATTCC AGGAAGGTGA AGAGAAGTTT GGCGCCTACA 
TTTGTCTCTC CTGTGAAGAC AGAAGGAAAC ATTTATAGTG TGAACGAAAG GTTATTCAAC 
GATTTCACAG AAGTAACACC AATTGGCTCA GGTGGATTTG GCCAAGTTTT CAAAGCCAAA 
CACAGAATTG ATAAGAAGAC TTATGTTATT AAATGTGTTA AATATAATAG TGAGAAGGTG 
GAGCGTGAAG TGAAAGCTTT GGCAACACTT AATCATCCAA ATATTGTTCA TTACCACAAT 
TGTTGGGATG GGCATGATTA TGATCCTGAG CAAAGCTTAA ATTCTTCAAG ATCAAAGACT 
AGGTGCCTTT TCATCCAGAT GGAATATTGT GATAAAGGTA CGTTGGACCA ATGGATTGAG 
AAAAGAAGAG GCAAGAAGCC AGACAAACGT TTGGCTTTGG ATTTCTTTCA ACAAATAACA 
ACAGGAGTGC ATTATATACA TTCAGAACAG TTAATTCATA GAGACCTTAA GCCAGGTAAC 
ATATTTTTAG TAGCTATGAA CCAAATCAAG ATTGGAGACT TTGGACTTGT TACATACCTG 
AAAAATGATG AAACGCGGAC AAGTAAAAAG GGAACTTTAC GATACATGAG CCCAGAACAG 
CTTTCTTCTG TGAAAGACTA TGGAAATGAA GTGGACATCT ATGCTTTGGG GCTGATTCTT 
GCAGAACTTC TTCATATATG TCTCACTTCT TTAGAAACAC AGAAGTTCTT TGACGATCTA 
AGGAATGGCA GATTAGATGT ATTTGACGAC AAAGAAAAAG ATCTTCTGGA GAAATTACTC 
TCAGTGGACC CCAAGAAACG ACCTACCGCA TCTGAGATAC TGAAGACTTT GAAGGAGTGG 
AATAATGTTA CAGAAAAAAA GAAACGAAAC ACATGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Modulation of PKR activity in cells infected by bovine viral diarrhea virus.
Virus research116(1-2)69-77(2006 Mar)
Gil LH,van Olphen AL,Mittal SK,Donis RO

The mRNA regulation of porcine double-stranded RNA-activated protein kinase gene.
The Journal of veterinary medical science66(12)1523-8(2004 Dec)
Asano A,Kon Y,Agui T

Tumour necrosis factor alpha up-regulates protein kinase R (PKR)-activating protein (PACT) and increases phosphorylation of PKR and eukaryotic initiation factor 2-alpha in articular chondrocytes.
Biochemical Society transactions30(Pt 6)886-9(2002 Nov)
Gilbert SJ,Duance VC,Mason DJ

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EIF2AK2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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