Prf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prf1
Entrez Gene ID	50669
Full Name	perforin 1
Synonyms	Cyta,RATCYTA
General protein information	Preferred Names perforin 1 Names perforin-1 P1 cytolysin lymphocyte pore-forming protein perforin 1 (pore forming protein)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q11
Summary	cytolytic factor present in cytoplasmic granules [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017330.2	NP_059026.2	perforin-1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRF1	NP_001076585.1
Xenopus tropicalis (tropical clawed frog)	LOC100489175	XP_002942088.2
Xenopus tropicalis (tropical clawed frog)	LOC101732838	XP_004919588.1
Xenopus tropicalis (tropical clawed frog)	LOC100489324	XP_002942089.2
Xenopus tropicalis (tropical clawed frog)	LOC100492973	XP_002941527.2
Xenopus tropicalis (tropical clawed frog)	LOC101732506	XP_004918449.1
Xenopus tropicalis (tropical clawed frog)	LOC100493300	XP_002941529.2
	PRF1	XP_001107909.1
Danio rerio (zebrafish)	prf1.7	XP_003200648.1
Danio rerio (zebrafish)	LOC100000903	XP_001341008.1
Xenopus tropicalis (tropical clawed frog)	LOC100489487	XP_002942090.2
Pan troglodytes (chimpanzee)	PRF1	XP_507837.2
Rattus norvegicus (Norway rat)	Prf1	NP_059026.2
Gallus gallus (chicken)	LOC101750363	XP_004945747.1
Danio rerio (zebrafish)	LOC569443	XP_697920.2
Xenopus tropicalis (tropical clawed frog)	LOC100491518	XP_002942100.2
Canis lupus familiaris (dog)	PRF1	NP_001184111.1
Bos taurus (cattle)	PRF1	NP_001137207.1
Mus musculus (house mouse)	Prf1	NP_035203.3
Xenopus tropicalis (tropical clawed frog)	LOC100493140	XP_002941528.1

Gene Ontology
Bibliography

Related articles in PubMed

Hypomethylation of perforin regulatory elements in CD4+ T cells from rat spleens contributes to the development of autoimmune emphysema.
Zhang C, Yan MY, Lu P, Chen P, Yang M, Ye XW, Xu M, Yang ZH, Zhang XY
Respirology (Carlton, Vic.)19(3)376-81(2014 Apr)

Defining the interaction of perforin with calcium and the phospholipid membrane.
Traore DA, Brennan AJ, Law RH, Dogovski C, Perugini MA, Lukoyanova N, Leung EW, Norton RS, Lopez JA, Browne KA, Yagita H, Lloyd GJ, Ciccone A, Verschoor S, Trapani JA, Whisstock JC, Voskoboinik I
The Biochemical journal456(3)323-35(2013 Dec)

Dietary mustard seeds (Sinapis alba Linn) suppress 1,2-dimethylhydrazine-induced immuno-imbalance and colonic carcinogenesis in rats.
Zhu M, Yuan H, Guo W, Li X, Jin L, Brunk UT, Han J, Zhao M, Lu Y
Nutrition and cancer64(3)464-72(2012 Apr)

Allergen-specific CTL require perforin expression to suppress allergic airway inflammation.
Enomoto N, Hyde E, Ma JZ, Yang J, Forbes-Blom E, Delahunt B, Le Gros G, Ronchese F
Journal of immunology (Baltimore, Md. : 1950)188(4)1734-41(2012 Feb)

Critical role of perforin-dependent CD8+ T cell immunity for rapid protective vaccination in a murine model for human smallpox.
Kremer M, Suezer Y, Volz A, Frenz T, Majzoub M, Hanschmann KM, Lehmann MH, Kalinke U, Sutter G
PLoS pathogens8(3)e1002557(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hypomethylation of perforin regulatory elements in CD4+ T cells may result in the lung septal cell apoptosis associated with the development of experimental autoimmune emphysema
Title: Hypomethylation of perforin regulatory elements in CD4+ T cells from rat spleens contributes to the development of autoimmune emphysema.

Defining the interaction of perforin with calcium and the phospholipid membrane.
Title: Defining the interaction of perforin with calcium and the phospholipid membrane.

Perforin and GzmB may play important roles in the pathogenesis of asthma.
Title: [Expression of perforin and granzyme B in asthmatic rats and intervention of recombinant human growth hormone].

In early stage of acute rejection of renal allograft in rats, the expressions of osteopontin, IL15, perforin and granzyme B are up-regulated.
Title: [Expressions of interleukin-15 and osteopontin mRNA in early stage of acute rejection of renal allograft in rats].

In antiglomerular basement membrane crescentic glomerulonephritis, glomerular mRNA expression of perforin and granzyme B corresponded with the number of CD8+ cells, whereas that of granzyme A, Fas, and FasL did not.
Title: Anti-perforin antibody treatment ameliorates experimental crescentic glomerulonephritis in WKY rats.

The following Prf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03666	NM_017330.2 Latest version!	Rattus norvegicus perforin 1 (Prf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prf1 ( NM_017330.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_059026.2 Rattus norvegicus perforin 1 (Prf1), mRNA.

Price & Availability↑

CloneID	ORa03666
Clone ID Related Accession (Same CDS sequence)	NM_017330.2
Accession Version	NM_017330.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	perforin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC089808.1. On Feb 13, 2005 this sequence version replaced NM_017330.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC089808.1, CO572485.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCGT ACCTGTTCCT CCTGGGCCTT TTCCTGCTGC TGCCACGACC TGTCCCTGCT 
CCCTGCTACA CTGCCACTCG GTCAGAATGC AAGCAGAAGC ACAAATTCGT GCCTGGTGTG 
TGGGCGGCTG GGGAAGGTGT GGATGTGACC ACCCTCCGCC GCTCCAGCTC CTTCCCAGTG 
AACACAGGGA AGTTCCTGAG GCCTGACCGC ACCTGCACCC TCTGTAAAAA CGCCCTGATG 
AATGACGCTA TACAGCGCCT ACCCGTGGCA ATCGCCCACT GGCGGCCTCA CGGCTCACAC 
TGCCAGCGTA ATGTGGCCAC AACCAAGGTC AGCTCCACGG AGGGTGTGGC GCGGGAGGCG 
GCTGCTAATA TCAATAACGA CTGGCGTGCG GGGCTGGATG TGAACCCTAA ACCGGAAGCA 
AACGTGCATG TGTCCGTGGC TGGCTCCCAT TCCAAGATAG CCAATTTTGC AGCCGAGAAG 
GCCCATCAGG ACCAATACAA CTTTAATACT GACACAGTGG AGTGTCGCAT GTACAGTTTT 
CGCCTGGCAC AAAAGCCTCC ACTCCACCCT GACTTCAGAA AGGCACTCAA GAACCTTCCC 
CACAACTTTA ACAGCTCCAC AGAGCATGCT TACCGTAGAC TCATCTCTTC CTATGGGACT 
CATTTTATTA CGGCTGTGGA CCTAGGTGGC CGCGTCTCGG TCCTCACGGC TCTGCGTACC 
TGTCAGCTGA CCCTGGATGG GCTCACAGCT GATGAGGTAG GAGACTGCTT GAGCGTGGAA 
GCCCAGGTCA GCATCGGTGC CCAAGCCAGT GTCTCAAGCG AATACAAAGC TTGCGAGGAG 
AAGAAGAAAC AACACAAAAT CGCCACCTCT TTCCACCAGA CCTACCGTGA GCGTCATGTC 
GAAGTGCTCG GTGGCCCCCT TGACTCCTCG AATGACCTGC TCTTCGGGAA CCAAGCTACC 
CCTGAGCACT TCTCTACCTG GATAGCCTCA TTGCCCACCA GGCCTGATGT GGTGGACTAC 
AGCCTGGAGC CCCTGCACAT ACTACTGGAA GACTCGGACC CGAAGCGAGA GGCACTGAGA 
CAGGCTATCA GCCATTATGT GATGAGCAGG GCTCGCTGGC GAGACTGTAA CCGGCCCTGC 
AGGGCAGGCC AGCATAAGAG TAGTCGTGAT TCATGCCAGT GTGTGTGCCA GGATTCGAAG 
GTCACCAACC AGGACTGCTG CCCACGCCAG AGGGGCTTGG CCAAGTTGAT GGTAAGGAAT 
TTCCAGGCCA AGGGTCTGTG GGGGGACTAC ATCACATCTA CGGATGCTTA TCTGAAGGTC 
TTCTTTGGTG GCCAGGAGAT CAGGACCGGT GTAGTGTGGA ACAATAACCA TCCCTCGTGG 
AGTGACAAGA TGGACTTTGG GAATGTGCTC CTGTCCACAG GGGGACCCCT CAGGGTGCAG 
GTCTGGGATG CTGACAATGG CTGGGATGAT GACCTTCTTG GTACTTGTGA CAAGTCTCCC 
AAGTCTGGTT TCCATGAGGT GAATTGTCCC CTGAACCACG GCAGTATAAA ATTCATCTAC 
CAGGCCAATT GCTTGCCCCA TCTCACTGGA GAGACCTGCC TGGAGTATGC CCCCCAGGGG 
CTTCTAGGAG ATCCACCAGG AAACCGCAGT GGGGCTGTGT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059026.2
CDS	53..1717
Translation	MAAYLFLLGLFLLLPRPVPAPCYTATRSECKQKHKFVPGVWAAGEGVDVTTLRRSSSFPVNTGKFLRPDRTCTLCKNALMNDAIQRLPVAIAHWRPHGSHCQRNVATTKVSSTEGVAREAAANINNDWRAGLDVNPKPEANVHVSVAGSHSKIANFAAEKAHQDQYNFNTDTVECRMYSFRLAQKPPLHPDFRKALKNLPHNFNSSTEHAYRRLISSYGTHFITAVDLGGRVSVLTALRTCQLTLDGLTADEVGDCLSVEAQVSIGAQASVSSEYKACEEKKKQHKIATSFHQTYRERHVEVLGGPLDSSNDLLFGNQATPEHFSTWIASLPTRPDVVDYSLEPLHILLEDSDPKREALRQAISHYVMSRARWRDCNRPCRAGQHKSSRDSCQCVCQDSKVTNQDCCPRQRGLAKLMVRNFQAKGLWGDYITSTDAYLKVFFGGQEIRTGVVWNNNHPSWSDKMDFGNVLLSTGGPLRVQVWDADNGWDDDLLGTCDKSPKSGFHEVNCPLNHGSIKFIYQANCLPHLTGETCLEYAPQGLLGDPPGNRSGAVW

Target ORF information:

RefSeq Version	NM_017330.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus perforin 1 (Prf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017330.2

ORF Insert Sequence:

ATGGCCGCGT ACCTGTTCCT CCTGGGCCTT TTCCTGCTGC TGCCACGACC TGTCCCTGCT 
CCCTGCTACA CTGCCACTCG GTCAGAATGC AAGCAGAAGC ACAAATTCGT GCCTGGTGTG 
TGGGCGGCTG GGGAAGGTGT GGATGTGACC ACCCTCCGCC GCTCCAGCTC CTTCCCAGTG 
AACACAGGGA AGTTCCTGAG GCCTGACCGC ACCTGCACCC TCTGTAAAAA CGCCCTGATG 
AATGACGCTA TACAGCGCCT ACCCGTGGCA ATCGCCCACT GGCGGCCTCA CGGCTCACAC 
TGCCAGCGTA ATGTGGCCAC AACCAAGGTC AGCTCCACGG AGGGTGTGGC GCGGGAGGCG 
GCTGCTAATA TCAATAACGA CTGGCGTGCG GGGCTGGATG TGAACCCTAA ACCGGAAGCA 
AACGTGCATG TGTCCGTGGC TGGCTCCCAT TCCAAGATAG CCAATTTTGC AGCCGAGAAG 
GCCCATCAGG ACCAATACAA CTTTAATACT GACACAGTGG AGTGTCGCAT GTACAGTTTT 
CGCCTGGCAC AAAAGCCTCC ACTCCACCCT GACTTCAGAA AGGCACTCAA GAACCTTCCC 
CACAACTTTA ACAGCTCCAC AGAGCATGCT TACCGTAGAC TCATCTCTTC CTATGGGACT 
CATTTTATTA CGGCTGTGGA CCTAGGTGGC CGCGTCTCGG TCCTCACGGC TCTGCGTACC 
TGTCAGCTGA CCCTGGATGG GCTCACAGCT GATGAGGTAG GAGACTGCTT GAGCGTGGAA 
GCCCAGGTCA GCATCGGTGC CCAAGCCAGT GTCTCAAGCG AATACAAAGC TTGCGAGGAG 
AAGAAGAAAC AACACAAAAT CGCCACCTCT TTCCACCAGA CCTACCGTGA GCGTCATGTC 
GAAGTGCTCG GTGGCCCCCT TGACTCCTCG AATGACCTGC TCTTCGGGAA CCAAGCTACC 
CCTGAGCACT TCTCTACCTG GATAGCCTCA TTGCCCACCA GGCCTGATGT GGTGGACTAC 
AGCCTGGAGC CCCTGCACAT ACTACTGGAA GACTCGGACC CGAAGCGAGA GGCACTGAGA 
CAGGCTATCA GCCATTATGT GATGAGCAGG GCTCGCTGGC GAGACTGTAA CCGGCCCTGC 
AGGGCAGGCC AGCATAAGAG TAGTCGTGAT TCATGCCAGT GTGTGTGCCA GGATTCGAAG 
GTCACCAACC AGGACTGCTG CCCACGCCAG AGGGGCTTGG CCAAGTTGAT GGTAAGGAAT 
TTCCAGGCCA AGGGTCTGTG GGGGGACTAC ATCACATCTA CGGATGCTTA TCTGAAGGTC 
TTCTTTGGTG GCCAGGAGAT CAGGACCGGT GTAGTGTGGA ACAATAACCA TCCCTCGTGG 
AGTGACAAGA TGGACTTTGG GAATGTGCTC CTGTCCACAG GGGGACCCCT CAGGGTGCAG 
GTCTGGGATG CTGACAATGG CTGGGATGAT GACCTTCTTG GTACTTGTGA CAAGTCTCCC 
AAGTCTGGTT TCCATGAGGT GAATTGTCCC CTGAACCACG GCAGTATAAA ATTCATCTAC 
CAGGCCAATT GCTTGCCCCA TCTCACTGGA GAGACCTGCC TGGAGTATGC CCCCCAGGGG 
CTTCTAGGAG ATCCACCAGG AAACCGCAGT GGGGCTGTGT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hypomethylation of perforin regulatory elements in CD4+ T cells from rat spleens contributes to the development of autoimmune emphysema.
Respirology (Carlton, Vic.)19(3)376-81(2014 Apr)
Zhang C,Yan MY,Lu P,Chen P,Yang M,Ye XW,Xu M,Yang ZH,Zhang XY

Defining the interaction of perforin with calcium and the phospholipid membrane.
The Biochemical journal456(3)323-35(2013 Dec)
Traore DA,Brennan AJ,Law RH,Dogovski C,Perugini MA,Lukoyanova N,Leung EW,Norton RS,Lopez JA,Browne KA,Yagita H,Lloyd GJ,Ciccone A,Verschoor S,Trapani JA,Whisstock JC,Voskoboinik I

Dietary mustard seeds (Sinapis alba Linn) suppress 1,2-dimethylhydrazine-induced immuno-imbalance and colonic carcinogenesis in rats.
Nutrition and cancer64(3)464-72(2012 Apr)
Zhu M,Yuan H,Guo W,Li X,Jin L,Brunk UT,Han J,Zhao M,Lu Y

Allergen-specific CTL require perforin expression to suppress allergic airway inflammation.
Journal of immunology (Baltimore, Md. : 1950)188(4)1734-41(2012 Feb)
Enomoto N,Hyde E,Ma JZ,Yang J,Forbes-Blom E,Delahunt B,Le Gros G,Ronchese F

Critical role of perforin-dependent CD8+ T cell immunity for rapid protective vaccination in a murine model for human smallpox.
PLoS pathogens8(3)e1002557(2012)
Kremer M,Suezer Y,Volz A,Frenz T,Majzoub M,Hanschmann KM,Lehmann MH,Kalinke U,Sutter G

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Prf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報