PRF1 cDNA ORF clone, Canis lupus familiaris(dog)

The following PRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe04868 NM_001197182.1
Latest version!
Canis lupus familiaris perforin 1 (PRF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OCe04868 XM_005618879.2
Latest version!
Canis lupus familiaris perforin 1 (PRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
View Old Accession Versions >>
OCe04868 XM_005618879.1 Canis lupus familiaris perforin 1 (pore forming protein) (PRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe04868
    Clone ID Related Accession (Same CDS sequence) XM_005618879.2 , XM_005618879.1 , NM_001197182.1
    Accession Version NM_001197182.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Canis lupus familiaris(dog)
    Product perforin-1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03002804.1 and FJ973622.1. On Oct 10, 2010 this sequence version replaced XM_546148.2. ##Evidence-Data-START## Transcript exon combination :: FJ973622.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA4547739 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGGTGCCC GCCTGTTCCT CCCGGGCCTC GTCCTGCTGC TGCTGCCCAC GCCCACCCCA 
    GCCCCCTGCC ACACGGCCAC GCGCAACGAG TGCCGGCGCA ACCGCTGGTT CGTGCCTGGC
    TCGATGCTGG CCGGGGAGGG CATGGACGTG ACCAGCCTCA AGCGCTCGGG CTCCTTCCCA
    GTCGACACAG AGAGCTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGCCG CAACACCCTA
    CGGGGGGGTG CCCTCCAGCG CCTGCCCCTG GCACTGACCG ACTGGCGCGC CCAGGGCTCG
    GGCTGCCAGC GCCGCGTGGT CAAGGCCAAG GCCAGCTCCA CCGAGGGTGT GGCCAGGGAA
    GCAGCCAGCA ACATCCACAA CGACTGGCGC GTGGGGCTGG ATGTGTCTCC CAAGCCCAAC
    ATCAATGTGC ATGTGAGCAT GGCCGGCTCG CACTCCAGGG TGGCCGACTT CGCTGCCCAG
    AAGACCCACC AGGACAGGTA CAGCTTCAGC ACTGACGTGG TGGAGTGTCG CTTCTACAGT
    TTCCACCTGG TACACTCTCC GCCACTGCAC CCCAATTTCC AGAGGGCCCT CAGCGACCTG
    CCCCCCAATT TCAACACCTC CACAGAGGCT GACTACCTCA GGCTCATCTC CAACTATGGC
    ACCCACTTCA TCCGGTCCAT GGAGCTGGGC GGCCGGACCC TGGCCCTCAC TGCCCTGCGT
    ACGTGTGAGC TGGCCCTGGA AGGGCTCACA GCCGATGAGG TGTCAGACTG CCTAACAGTC
    GAGGCCCAGG TCAGCATAAG TGACCACGCC AACCCTTCAT CAGAATTCAA GGCCTGTGAG
    GAAAAGAAGA AGAAGCACAA GATGACAACC TCCTTCCACC AGGCCTACCA GGAACGCTTT
    TCCGAGATAA TTGGTGGCCA CCATACCTCC ATGAGTGACC TGCTGTTTGG GAACCAGGCC
    GGGCCGGAGC AGTTCTCAGC CTGGATGGCC TCACTGCTGG ACAGACCCGG CCTGGTGGAC
    TACACCCTGG AGCCTCTGCA TGTGCTCCTG GAGAGCCAGG ACCCACGGCG GGAGGCGCTG
    AGGCAGGCTG TGAGCAAGTA CGTGATGGAC AGAGCCCGTT GGAAGAACTG CAGCAGGCCC
    TGCCCCCCGG GGCAGCAGAA GAGCCCCCAC AACCCATGCC ACTGTGTGTG CCACAACTCG
    GGGATCACCA ACCAGGACTG CTGTCCCCGG CAGAGGGGCC TGGCCCACTT AGAGGTTATG
    AACTTCCAGG CAATGGGCCT GTGGGGAGAC TACTTCACGG CCACAGATGC CTATCTGAAA
    GTCTTCTTTG GAGGCCAGGA GCAGAGAACC TACACGGTGT GGAACAATAA TAACCCCACG
    TGGGTGACGC GGCTGGACTT CGGGAACGTG CTCCTGTCCA CGGGGGGGCC CCTGAGGGTT
    CAGGTCTGGG ACCAAGACTA TGGCTGGGAT GATGACCTCC TTGGCACCTG TGACCAGAAA
    GCACAGTCTG GCTCACATGA GGTCAGGTGC AACCTGTCCC ACGGTCACCT GAGATTCTCC
    TACATTGCCA AGTGCTTGCC TCACCTGACG GGGGCAACCT GCCTGGAGTA TGCCCCCCAT
    GGTCGTCTGG GAGAGCCTCC AGGAAACCGG AGTGGGCCAG TGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001184111.1
    CDS5..1672
    Translation

    Target ORF information:

    RefSeq Version NM_001197182.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris perforin 1 (PRF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001197182.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGGTGCCC GCCTGTTCCT CCCGGGCCTC GTCCTGCTGC TGCTGCCCAC GCCCACCCCA 
    GCCCCCTGCC ACACGGCCAC GCGCAACGAG TGCCGGCGCA ACCGCTGGTT CGTGCCTGGC
    TCGATGCTGG CCGGGGAGGG CATGGACGTG ACCAGCCTCA AGCGCTCGGG CTCCTTCCCA
    GTCGACACAG AGAGCTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGCCG CAACACCCTA
    CGGGGGGGTG CCCTCCAGCG CCTGCCCCTG GCACTGACCG ACTGGCGCGC CCAGGGCTCG
    GGCTGCCAGC GCCGCGTGGT CAAGGCCAAG GCCAGCTCCA CCGAGGGTGT GGCCAGGGAA
    GCAGCCAGCA ACATCCACAA CGACTGGCGC GTGGGGCTGG ATGTGTCTCC CAAGCCCAAC
    ATCAATGTGC ATGTGAGCAT GGCCGGCTCG CACTCCAGGG TGGCCGACTT CGCTGCCCAG
    AAGACCCACC AGGACAGGTA CAGCTTCAGC ACTGACGTGG TGGAGTGTCG CTTCTACAGT
    TTCCACCTGG TACACTCTCC GCCACTGCAC CCCAATTTCC AGAGGGCCCT CAGCGACCTG
    CCCCCCAATT TCAACACCTC CACAGAGGCT GACTACCTCA GGCTCATCTC CAACTATGGC
    ACCCACTTCA TCCGGTCCAT GGAGCTGGGC GGCCGGACCC TGGCCCTCAC TGCCCTGCGT
    ACGTGTGAGC TGGCCCTGGA AGGGCTCACA GCCGATGAGG TGTCAGACTG CCTAACAGTC
    GAGGCCCAGG TCAGCATAAG TGACCACGCC AACCCTTCAT CAGAATTCAA GGCCTGTGAG
    GAAAAGAAGA AGAAGCACAA GATGACAACC TCCTTCCACC AGGCCTACCA GGAACGCTTT
    TCCGAGATAA TTGGTGGCCA CCATACCTCC ATGAGTGACC TGCTGTTTGG GAACCAGGCC
    GGGCCGGAGC AGTTCTCAGC CTGGATGGCC TCACTGCTGG ACAGACCCGG CCTGGTGGAC
    TACACCCTGG AGCCTCTGCA TGTGCTCCTG GAGAGCCAGG ACCCACGGCG GGAGGCGCTG
    AGGCAGGCTG TGAGCAAGTA CGTGATGGAC AGAGCCCGTT GGAAGAACTG CAGCAGGCCC
    TGCCCCCCGG GGCAGCAGAA GAGCCCCCAC AACCCATGCC ACTGTGTGTG CCACAACTCG
    GGGATCACCA ACCAGGACTG CTGTCCCCGG CAGAGGGGCC TGGCCCACTT AGAGGTTATG
    AACTTCCAGG CAATGGGCCT GTGGGGAGAC TACTTCACGG CCACAGATGC CTATCTGAAA
    GTCTTCTTTG GAGGCCAGGA GCAGAGAACC TACACGGTGT GGAACAATAA TAACCCCACG
    TGGGTGACGC GGCTGGACTT CGGGAACGTG CTCCTGTCCA CGGGGGGGCC CCTGAGGGTT
    CAGGTCTGGG ACCAAGACTA TGGCTGGGAT GATGACCTCC TTGGCACCTG TGACCAGAAA
    GCACAGTCTG GCTCACATGA GGTCAGGTGC AACCTGTCCC ACGGTCACCT GAGATTCTCC
    TACATTGCCA AGTGCTTGCC TCACCTGACG GGGGCAACCT GCCTGGAGTA TGCCCCCCAT
    GGTCGTCTGG GAGAGCCTCC AGGAAACCGG AGTGGGCCAG TGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04868
    Clone ID Related Accession (Same CDS sequence) XM_005618879.2 , XM_005618879.1 , NM_001197182.1
    Accession Version XM_005618879.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product perforin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618879.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGGTGCCC GCCTGTTCCT CCCGGGCCTC GTCCTGCTGC TGCTGCCCAC GCCCACCCCA 
    GCCCCCTGCC ACACGGCCAC GCGCAACGAG TGCCGGCGCA ACCGCTGGTT CGTGCCTGGC
    TCGATGCTGG CCGGGGAGGG CATGGACGTG ACCAGCCTCA AGCGCTCGGG CTCCTTCCCA
    GTCGACACAG AGAGCTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGCCG CAACACCCTA
    CGGGGGGGTG CCCTCCAGCG CCTGCCCCTG GCACTGACCG ACTGGCGCGC CCAGGGCTCG
    GGCTGCCAGC GCCGCGTGGT CAAGGCCAAG GCCAGCTCCA CCGAGGGTGT GGCCAGGGAA
    GCAGCCAGCA ACATCCACAA CGACTGGCGC GTGGGGCTGG ATGTGTCTCC CAAGCCCAAC
    ATCAATGTGC ATGTGAGCAT GGCCGGCTCG CACTCCAGGG TGGCCGACTT CGCTGCCCAG
    AAGACCCACC AGGACAGGTA CAGCTTCAGC ACTGACGTGG TGGAGTGTCG CTTCTACAGT
    TTCCACCTGG TACACTCTCC GCCACTGCAC CCCAATTTCC AGAGGGCCCT CAGCGACCTG
    CCCCCCAATT TCAACACCTC CACAGAGGCT GACTACCTCA GGCTCATCTC CAACTATGGC
    ACCCACTTCA TCCGGTCCAT GGAGCTGGGC GGCCGGACCC TGGCCCTCAC TGCCCTGCGT
    ACGTGTGAGC TGGCCCTGGA AGGGCTCACA GCCGATGAGG TGTCAGACTG CCTAACAGTC
    GAGGCCCAGG TCAGCATAAG TGACCACGCC AACCCTTCAT CAGAATTCAA GGCCTGTGAG
    GAAAAGAAGA AGAAGCACAA GATGACAACC TCCTTCCACC AGGCCTACCA GGAACGCTTT
    TCCGAGATAA TTGGTGGCCA CCATACCTCC ATGAGTGACC TGCTGTTTGG GAACCAGGCC
    GGGCCGGAGC AGTTCTCAGC CTGGATGGCC TCACTGCTGG ACAGACCCGG CCTGGTGGAC
    TACACCCTGG AGCCTCTGCA TGTGCTCCTG GAGAGCCAGG ACCCACGGCG GGAGGCGCTG
    AGGCAGGCTG TGAGCAAGTA CGTGATGGAC AGAGCCCGTT GGAAGAACTG CAGCAGGCCC
    TGCCCCCCGG GGCAGCAGAA GAGCCCCCAC AACCCATGCC ACTGTGTGTG CCACAACTCG
    GGGATCACCA ACCAGGACTG CTGTCCCCGG CAGAGGGGCC TGGCCCACTT AGAGGTTATG
    AACTTCCAGG CAATGGGCCT GTGGGGAGAC TACTTCACGG CCACAGATGC CTATCTGAAA
    GTCTTCTTTG GAGGCCAGGA GCAGAGAACC TACACGGTGT GGAACAATAA TAACCCCACG
    TGGGTGACGC GGCTGGACTT CGGGAACGTG CTCCTGTCCA CGGGGGGGCC CCTGAGGGTT
    CAGGTCTGGG ACCAAGACTA TGGCTGGGAT GATGACCTCC TTGGCACCTG TGACCAGAAA
    GCACAGTCTG GCTCACATGA GGTCAGGTGC AACCTGTCCC ACGGTCACCT GAGATTCTCC
    TACATTGCCA AGTGCTTGCC TCACCTGACG GGGGCAACCT GCCTGGAGTA TGCCCCCCAT
    GGTCGTCTGG GAGAGCCTCC AGGAAACCGG AGTGGGCCAG TGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618936.1
    CDS133..1800
    Translation

    Target ORF information:

    RefSeq Version XM_005618879.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris perforin 1 (PRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618879.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGGTGCCC GCCTGTTCCT CCCGGGCCTC GTCCTGCTGC TGCTGCCCAC GCCCACCCCA 
    GCCCCCTGCC ACACGGCCAC GCGCAACGAG TGCCGGCGCA ACCGCTGGTT CGTGCCTGGC
    TCGATGCTGG CCGGGGAGGG CATGGACGTG ACCAGCCTCA AGCGCTCGGG CTCCTTCCCA
    GTCGACACAG AGAGCTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGCCG CAACACCCTA
    CGGGGGGGTG CCCTCCAGCG CCTGCCCCTG GCACTGACCG ACTGGCGCGC CCAGGGCTCG
    GGCTGCCAGC GCCGCGTGGT CAAGGCCAAG GCCAGCTCCA CCGAGGGTGT GGCCAGGGAA
    GCAGCCAGCA ACATCCACAA CGACTGGCGC GTGGGGCTGG ATGTGTCTCC CAAGCCCAAC
    ATCAATGTGC ATGTGAGCAT GGCCGGCTCG CACTCCAGGG TGGCCGACTT CGCTGCCCAG
    AAGACCCACC AGGACAGGTA CAGCTTCAGC ACTGACGTGG TGGAGTGTCG CTTCTACAGT
    TTCCACCTGG TACACTCTCC GCCACTGCAC CCCAATTTCC AGAGGGCCCT CAGCGACCTG
    CCCCCCAATT TCAACACCTC CACAGAGGCT GACTACCTCA GGCTCATCTC CAACTATGGC
    ACCCACTTCA TCCGGTCCAT GGAGCTGGGC GGCCGGACCC TGGCCCTCAC TGCCCTGCGT
    ACGTGTGAGC TGGCCCTGGA AGGGCTCACA GCCGATGAGG TGTCAGACTG CCTAACAGTC
    GAGGCCCAGG TCAGCATAAG TGACCACGCC AACCCTTCAT CAGAATTCAA GGCCTGTGAG
    GAAAAGAAGA AGAAGCACAA GATGACAACC TCCTTCCACC AGGCCTACCA GGAACGCTTT
    TCCGAGATAA TTGGTGGCCA CCATACCTCC ATGAGTGACC TGCTGTTTGG GAACCAGGCC
    GGGCCGGAGC AGTTCTCAGC CTGGATGGCC TCACTGCTGG ACAGACCCGG CCTGGTGGAC
    TACACCCTGG AGCCTCTGCA TGTGCTCCTG GAGAGCCAGG ACCCACGGCG GGAGGCGCTG
    AGGCAGGCTG TGAGCAAGTA CGTGATGGAC AGAGCCCGTT GGAAGAACTG CAGCAGGCCC
    TGCCCCCCGG GGCAGCAGAA GAGCCCCCAC AACCCATGCC ACTGTGTGTG CCACAACTCG
    GGGATCACCA ACCAGGACTG CTGTCCCCGG CAGAGGGGCC TGGCCCACTT AGAGGTTATG
    AACTTCCAGG CAATGGGCCT GTGGGGAGAC TACTTCACGG CCACAGATGC CTATCTGAAA
    GTCTTCTTTG GAGGCCAGGA GCAGAGAACC TACACGGTGT GGAACAATAA TAACCCCACG
    TGGGTGACGC GGCTGGACTT CGGGAACGTG CTCCTGTCCA CGGGGGGGCC CCTGAGGGTT
    CAGGTCTGGG ACCAAGACTA TGGCTGGGAT GATGACCTCC TTGGCACCTG TGACCAGAAA
    GCACAGTCTG GCTCACATGA GGTCAGGTGC AACCTGTCCC ACGGTCACCT GAGATTCTCC
    TACATTGCCA AGTGCTTGCC TCACCTGACG GGGGCAACCT GCCTGGAGTA TGCCCCCCAT
    GGTCGTCTGG GAGAGCCTCC AGGAAACCGG AGTGGGCCAG TGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04868
    Clone ID Related Accession (Same CDS sequence) XM_005618879.2 , XM_005618879.1 , NM_001197182.1
    Accession Version XM_005618879.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product perforin-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGGTGCCC GCCTGTTCCT CCCGGGCCTC GTCCTGCTGC TGCTGCCCAC GCCCACCCCA 
    GCCCCCTGCC ACACGGCCAC GCGCAACGAG TGCCGGCGCA ACCGCTGGTT CGTGCCTGGC
    TCGATGCTGG CCGGGGAGGG CATGGACGTG ACCAGCCTCA AGCGCTCGGG CTCCTTCCCA
    GTCGACACAG AGAGCTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGCCG CAACACCCTA
    CGGGGGGGTG CCCTCCAGCG CCTGCCCCTG GCACTGACCG ACTGGCGCGC CCAGGGCTCG
    GGCTGCCAGC GCCGCGTGGT CAAGGCCAAG GCCAGCTCCA CCGAGGGTGT GGCCAGGGAA
    GCAGCCAGCA ACATCCACAA CGACTGGCGC GTGGGGCTGG ATGTGTCTCC CAAGCCCAAC
    ATCAATGTGC ATGTGAGCAT GGCCGGCTCG CACTCCAGGG TGGCCGACTT CGCTGCCCAG
    AAGACCCACC AGGACAGGTA CAGCTTCAGC ACTGACGTGG TGGAGTGTCG CTTCTACAGT
    TTCCACCTGG TACACTCTCC GCCACTGCAC CCCAATTTCC AGAGGGCCCT CAGCGACCTG
    CCCCCCAATT TCAACACCTC CACAGAGGCT GACTACCTCA GGCTCATCTC CAACTATGGC
    ACCCACTTCA TCCGGTCCAT GGAGCTGGGC GGCCGGACCC TGGCCCTCAC TGCCCTGCGT
    ACGTGTGAGC TGGCCCTGGA AGGGCTCACA GCCGATGAGG TGTCAGACTG CCTAACAGTC
    GAGGCCCAGG TCAGCATAAG TGACCACGCC AACCCTTCAT CAGAATTCAA GGCCTGTGAG
    GAAAAGAAGA AGAAGCACAA GATGACAACC TCCTTCCACC AGGCCTACCA GGAACGCTTT
    TCCGAGATAA TTGGTGGCCA CCATACCTCC ATGAGTGACC TGCTGTTTGG GAACCAGGCC
    GGGCCGGAGC AGTTCTCAGC CTGGATGGCC TCACTGCTGG ACAGACCCGG CCTGGTGGAC
    TACACCCTGG AGCCTCTGCA TGTGCTCCTG GAGAGCCAGG ACCCACGGCG GGAGGCGCTG
    AGGCAGGCTG TGAGCAAGTA CGTGATGGAC AGAGCCCGTT GGAAGAACTG CAGCAGGCCC
    TGCCCCCCGG GGCAGCAGAA GAGCCCCCAC AACCCATGCC ACTGTGTGTG CCACAACTCG
    GGGATCACCA ACCAGGACTG CTGTCCCCGG CAGAGGGGCC TGGCCCACTT AGAGGTTATG
    AACTTCCAGG CAATGGGCCT GTGGGGAGAC TACTTCACGG CCACAGATGC CTATCTGAAA
    GTCTTCTTTG GAGGCCAGGA GCAGAGAACC TACACGGTGT GGAACAATAA TAACCCCACG
    TGGGTGACGC GGCTGGACTT CGGGAACGTG CTCCTGTCCA CGGGGGGGCC CCTGAGGGTT
    CAGGTCTGGG ACCAAGACTA TGGCTGGGAT GATGACCTCC TTGGCACCTG TGACCAGAAA
    GCACAGTCTG GCTCACATGA GGTCAGGTGC AACCTGTCCC ACGGTCACCT GAGATTCTCC
    TACATTGCCA AGTGCTTGCC TCACCTGACG GGGGCAACCT GCCTGGAGTA TGCCCCCCAT
    GGTCGTCTGG GAGAGCCTCC AGGAAACCGG AGTGGGCCAG TGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618936.1
    CDS123..1790
    Translation

    Target ORF information:

    RefSeq Version XM_005618879.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris perforin 1 (pore forming protein) (PRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618879.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGGTGCCC GCCTGTTCCT CCCGGGCCTC GTCCTGCTGC TGCTGCCCAC GCCCACCCCA 
    GCCCCCTGCC ACACGGCCAC GCGCAACGAG TGCCGGCGCA ACCGCTGGTT CGTGCCTGGC
    TCGATGCTGG CCGGGGAGGG CATGGACGTG ACCAGCCTCA AGCGCTCGGG CTCCTTCCCA
    GTCGACACAG AGAGCTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGCCG CAACACCCTA
    CGGGGGGGTG CCCTCCAGCG CCTGCCCCTG GCACTGACCG ACTGGCGCGC CCAGGGCTCG
    GGCTGCCAGC GCCGCGTGGT CAAGGCCAAG GCCAGCTCCA CCGAGGGTGT GGCCAGGGAA
    GCAGCCAGCA ACATCCACAA CGACTGGCGC GTGGGGCTGG ATGTGTCTCC CAAGCCCAAC
    ATCAATGTGC ATGTGAGCAT GGCCGGCTCG CACTCCAGGG TGGCCGACTT CGCTGCCCAG
    AAGACCCACC AGGACAGGTA CAGCTTCAGC ACTGACGTGG TGGAGTGTCG CTTCTACAGT
    TTCCACCTGG TACACTCTCC GCCACTGCAC CCCAATTTCC AGAGGGCCCT CAGCGACCTG
    CCCCCCAATT TCAACACCTC CACAGAGGCT GACTACCTCA GGCTCATCTC CAACTATGGC
    ACCCACTTCA TCCGGTCCAT GGAGCTGGGC GGCCGGACCC TGGCCCTCAC TGCCCTGCGT
    ACGTGTGAGC TGGCCCTGGA AGGGCTCACA GCCGATGAGG TGTCAGACTG CCTAACAGTC
    GAGGCCCAGG TCAGCATAAG TGACCACGCC AACCCTTCAT CAGAATTCAA GGCCTGTGAG
    GAAAAGAAGA AGAAGCACAA GATGACAACC TCCTTCCACC AGGCCTACCA GGAACGCTTT
    TCCGAGATAA TTGGTGGCCA CCATACCTCC ATGAGTGACC TGCTGTTTGG GAACCAGGCC
    GGGCCGGAGC AGTTCTCAGC CTGGATGGCC TCACTGCTGG ACAGACCCGG CCTGGTGGAC
    TACACCCTGG AGCCTCTGCA TGTGCTCCTG GAGAGCCAGG ACCCACGGCG GGAGGCGCTG
    AGGCAGGCTG TGAGCAAGTA CGTGATGGAC AGAGCCCGTT GGAAGAACTG CAGCAGGCCC
    TGCCCCCCGG GGCAGCAGAA GAGCCCCCAC AACCCATGCC ACTGTGTGTG CCACAACTCG
    GGGATCACCA ACCAGGACTG CTGTCCCCGG CAGAGGGGCC TGGCCCACTT AGAGGTTATG
    AACTTCCAGG CAATGGGCCT GTGGGGAGAC TACTTCACGG CCACAGATGC CTATCTGAAA
    GTCTTCTTTG GAGGCCAGGA GCAGAGAACC TACACGGTGT GGAACAATAA TAACCCCACG
    TGGGTGACGC GGCTGGACTT CGGGAACGTG CTCCTGTCCA CGGGGGGGCC CCTGAGGGTT
    CAGGTCTGGG ACCAAGACTA TGGCTGGGAT GATGACCTCC TTGGCACCTG TGACCAGAAA
    GCACAGTCTG GCTCACATGA GGTCAGGTGC AACCTGTCCC ACGGTCACCT GAGATTCTCC
    TACATTGCCA AGTGCTTGCC TCACCTGACG GGGGCAACCT GCCTGGAGTA TGCCCCCCAT
    GGTCGTCTGG GAGAGCCTCC AGGAAACCGG AGTGGGCCAG TGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.