PRF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRF1
Entrez Gene ID	5551
Full Name	perforin 1
Synonyms	HPLH2,P1,PFP
General protein information	Preferred Names perforin 1 Names perforin-1 cytolysin lymphocyte pore-forming protein perforin 1 (pore forming protein)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q22.1
Summary	This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017].
Disorder MIM:	170280
Disorder Html:	Hemophagocytic lymphohistiocytosis, familial, 2, 603553 (3); Lymphoma, non-Hodgkin, 605027 (3); Aplastic anemia, 609135 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001083116.1	NP_001076585.1	perforin-1 precursor
NM_005041.5	NP_005032.2	perforin-1 precursor
NM_005041.4	NP_005032.2	perforin-1 precursor
NM_001083116.3	NP_001076585.1	perforin-1 precursor
NM_001083116.2	NP_001076585.1	perforin-1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRF1	NP_001076585.1
Xenopus tropicalis (tropical clawed frog)	LOC100489175	XP_002942088.2
Xenopus tropicalis (tropical clawed frog)	LOC101732838	XP_004919588.1
Xenopus tropicalis (tropical clawed frog)	LOC100489324	XP_002942089.2
Xenopus tropicalis (tropical clawed frog)	LOC100492973	XP_002941527.2
Xenopus tropicalis (tropical clawed frog)	LOC101732506	XP_004918449.1
Xenopus tropicalis (tropical clawed frog)	LOC100493300	XP_002941529.2
	PRF1	XP_001107909.1
Danio rerio (zebrafish)	prf1.7	XP_003200648.1
Danio rerio (zebrafish)	LOC100000903	XP_001341008.1
Xenopus tropicalis (tropical clawed frog)	LOC100489487	XP_002942090.2
Pan troglodytes (chimpanzee)	PRF1	XP_507837.2
Rattus norvegicus (Norway rat)	Prf1	NP_059026.2
Gallus gallus (chicken)	LOC101750363	XP_004945747.1
Danio rerio (zebrafish)	LOC569443	XP_697920.2
Xenopus tropicalis (tropical clawed frog)	LOC100491518	XP_002942100.2
Canis lupus familiaris (dog)	PRF1	NP_001184111.1
Bos taurus (cattle)	PRF1	NP_001137207.1
Mus musculus (house mouse)	Prf1	NP_035203.3
Xenopus tropicalis (tropical clawed frog)	LOC100493140	XP_002941528.1

Gene Ontology
Bibliography

Related articles in PubMed

Perforin gene variation influences survival in childhood acute lymphoblastic leukemia.
Jaworowska A, Pastorczak A, Trelinska J, Wypyszczak K, Borowiec M, Fendler W, Sedek L, Szczepanski T, Ploski R, M?ynarski W
Leukemia research6529-33(2018 Jan)

Regulation of perforin activation and pre-synaptic toxicity through C-terminal glycosylation.
House IG, House CM, Brennan AJ, Gilan O, Dawson MA, Whisstock JC, Law RH, Trapani JA, Voskoboinik I
EMBO reports18(10)1775-1785(2017 Oct)

Finding a Balance between Protection and Pathology: The Dual Role of Perforin in Human Disease.
Willenbring RC, Johnson AJ
International journal of molecular sciences18(8)(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutation in PRF1 gene is associated with hemophagocytic lymphohistiocytosis.
Title: Clinical presentation and outcome of pediatric patients with hemophagocytic lymphohistiocytosis in China: A retrospective multicenter study.

Findings support an association between childhood DNA methylation patterns in PRF1 and a record of severe respiratory syncytial virus bronchiolitis in infancy.
Title: Reduced PRF1 enhancer methylation in children with a history of severe RSV bronchiolitis in infancy: an association study.

our data provide the first evidence of a strict link between the absence of CD28 and the expression of perforin, which is likewise enhanced by the expression of NKG2D, within selected CD4(+) T cells from cervical cancer patients.
Title: Loss of CD28 within CD4

Increased TIM3+CD8+T cells with lower perforin and granzyme B expression and higher CD95 expression in MDS patients were observed.
Title: Increased TIM3+CD8+T cells in Myelodysplastic Syndrome patients displayed less perforin and granzyme B secretion and higher CD95 expression.

Our studies suggest that although moderate levels of expression can result in partial amelioration of the hemophagocytic lymphohistiocytosis (HLH)phenotype, high levels of perforin expression per cell are required for complete correction of HLH.
Title: High Level of Perforin Expression Is Required for Effective Correction of Hemophagocytic Lymphohistiocytosis.

The following PRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23295	NM_001083116.3 Latest version!	Homo sapiens perforin 1 (PRF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu23295	NM_005041.5 Latest version!	Homo sapiens perforin 1 (PRF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
View Old Accession Versions >>
OHu23295	NM_005041.4	Homo sapiens perforin 1 (PRF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu23295	NM_001083116.1	Homo sapiens perforin 1 (PRF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu23295	NM_001083116.2	Homo sapiens perforin 1 (PRF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRF1 ( NM_001083116.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001076585.1 Homo sapiens perforin 1 (PRF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu23295
Clone ID Related Accession (Same CDS sequence)	NM_001083116.1 , NM_005041.5 , NM_005041.4 , NM_001083116.3 , NM_001083116.2
Accession Version	NM_001083116.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-05
Organism	Homo sapiens(Human)
Product	perforin-1 precursor
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA941244.1, X13224.1, BC047695.1 and BQ654088.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2155751, SAMN01820691 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT 
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA 
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA 
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT 
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT 
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC 
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG 
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT 
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG 
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC 
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC 
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC 
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG 
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC 
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC 
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC 
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG 
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG 
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA 
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC 
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG 
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC 
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG 
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT 
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC 
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA 
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076585.1
CDS	162..1829
Translation	MAARLLLLGILLLLLPLPVPAPCHTAARSECKRSHKFVPGAWLAGEGVDVTSLRRSGSFPVDTQRFLRPDGTCTLCENALQEGTLQRLPLALTNWRAQGSGCQRHVTRAKVSSTEAVARDAARSIRNDWKVGLDVTPKPTSNVHVSVAGSHSQAANFAAQKTHQDQYSFSTDTVECRFYSFHVVHTPPLHPDFKRALGDLPHHFNASTQPAYLRLISNYGTHFIRAVELGGRISALTALRTCELALEGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACEEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLLDSQDPRREALRRALSQYLTDRARWRDCSRPCPPGRQKSPRDPCQCVCHGSAVTTQDCCPRQRGLAQLEVTFIQAWGLWGDWFTATDAYVKLFFGGQELRTSTVWDNNNPIWSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEVRCNLNHGHLKFRYHARCLPHLGGGTCLDYVPQMLLGEPPGNRSGAVW

Target ORF information:

RefSeq Version	NM_001083116.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens perforin 1 (PRF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083116.1

ORF Insert Sequence:

ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT 
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA 
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA 
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT 
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT 
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC 
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG 
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT 
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG 
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC 
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC 
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC 
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG 
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC 
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC 
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC 
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG 
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG 
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA 
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC 
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG 
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC 
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG 
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT 
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC 
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA 
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRF1 ( NM_005041.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005032.2 Homo sapiens perforin 1 (PRF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23295
Clone ID Related Accession (Same CDS sequence)	NM_001083116.1 , NM_005041.5 , NM_005041.4 , NM_001083116.3 , NM_001083116.2
Accession Version	NM_005041.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	perforin-1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY075867.1, DA816891.1, X13224.1, BC047695.1 and BQ654088.1. On Aug 31, 2017 this sequence version replaced NM_005041.4. Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ555237.1, SRR1163657.274701.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT 
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA 
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA 
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT 
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT 
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC 
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG 
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT 
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG 
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC 
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC 
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC 
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG 
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC 
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC 
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC 
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG 
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG 
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA 
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC 
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG 
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC 
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG 
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT 
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC 
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA 
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005032.2
CDS	149..1816
Translation	MAARLLLLGILLLLLPLPVPAPCHTAARSECKRSHKFVPGAWLAGEGVDVTSLRRSGSFPVDTQRFLRPDGTCTLCENALQEGTLQRLPLALTNWRAQGSGCQRHVTRAKVSSTEAVARDAARSIRNDWKVGLDVTPKPTSNVHVSVAGSHSQAANFAAQKTHQDQYSFSTDTVECRFYSFHVVHTPPLHPDFKRALGDLPHHFNASTQPAYLRLISNYGTHFIRAVELGGRISALTALRTCELALEGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACEEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLLDSQDPRREALRRALSQYLTDRARWRDCSRPCPPGRQKSPRDPCQCVCHGSAVTTQDCCPRQRGLAQLEVTFIQAWGLWGDWFTATDAYVKLFFGGQELRTSTVWDNNNPIWSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEVRCNLNHGHLKFRYHARCLPHLGGGTCLDYVPQMLLGEPPGNRSGAVW

Target ORF information:

RefSeq Version	NM_005041.5
Organism	Homo sapiens(human)
Definition	Homo sapiens perforin 1 (PRF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005041.5

ORF Insert Sequence:

ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT 
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA 
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA 
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT 
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT 
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC 
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG 
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT 
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG 
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC 
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC 
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC 
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG 
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC 
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC 
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC 
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG 
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG 
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA 
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC 
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG 
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC 
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG 
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT 
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC 
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA 
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRF1 ( NM_005041.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005032.2 Homo sapiens perforin 1 (PRF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23295
Clone ID Related Accession (Same CDS sequence)	NM_001083116.1 , NM_005041.5 , NM_005041.4 , NM_001083116.3 , NM_001083116.2
Accession Version	NM_005041.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-28
Organism	Homo sapiens(Human)
Product	perforin-1 precursor
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA816891.1, X13224.1, BC047695.1 and BQ654088.1. On Mar 21, 2007 this sequence version replaced NM_005041.3. Transcript Variant: This variant (1) represents the shorter transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ555237.1, SRR1163657.274701.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT 
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA 
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA 
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT 
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT 
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC 
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG 
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT 
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG 
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC 
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC 
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC 
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG 
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC 
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC 
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC 
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG 
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG 
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA 
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC 
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG 
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC 
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG 
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT 
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC 
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA 
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005032.2
CDS	136..1803
Translation	MAARLLLLGILLLLLPLPVPAPCHTAARSECKRSHKFVPGAWLAGEGVDVTSLRRSGSFPVDTQRFLRPDGTCTLCENALQEGTLQRLPLALTNWRAQGSGCQRHVTRAKVSSTEAVARDAARSIRNDWKVGLDVTPKPTSNVHVSVAGSHSQAANFAAQKTHQDQYSFSTDTVECRFYSFHVVHTPPLHPDFKRALGDLPHHFNASTQPAYLRLISNYGTHFIRAVELGGRISALTALRTCELALEGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACEEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLLDSQDPRREALRRALSQYLTDRARWRDCSRPCPPGRQKSPRDPCQCVCHGSAVTTQDCCPRQRGLAQLEVTFIQAWGLWGDWFTATDAYVKLFFGGQELRTSTVWDNNNPIWSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEVRCNLNHGHLKFRYHARCLPHLGGGTCLDYVPQMLLGEPPGNRSGAVW

Target ORF information:

RefSeq Version	NM_005041.4
Organism	Homo sapiens(Human)
Definition	Homo sapiens perforin 1 (PRF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005041.4

ORF Insert Sequence:

ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT 
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA 
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA 
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT 
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT 
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC 
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG 
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT 
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG 
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC 
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC 
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC 
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG 
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC 
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC 
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC 
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG 
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG 
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA 
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC 
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG 
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC 
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG 
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT 
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC 
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA 
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRF1 ( NM_001083116.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001076585.1 Homo sapiens perforin 1 (PRF1), transcript variant 2, mRNA.

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CloneID	OHu23295
Clone ID Related Accession (Same CDS sequence)	NM_001083116.1 , NM_005041.5 , NM_005041.4 , NM_001083116.3 , NM_001083116.2
Accession Version	NM_001083116.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	perforin-1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA941244.1, X13224.1, BC047695.1 and BQ654088.1. On Nov 23, 2018 this sequence version replaced NM_001083116.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279851.3847.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155751, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000441259.2/ ENSP00000398568.1 RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT 
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA 
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA 
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT 
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT 
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC 
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG 
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT 
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG 
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC 
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC 
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC 
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG 
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC 
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC 
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC 
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG 
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG 
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA 
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC 
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG 
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC 
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG 
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT 
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC 
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA 
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076585.1
CDS	128..1795
Translation	MAARLLLLGILLLLLPLPVPAPCHTAARSECKRSHKFVPGAWLAGEGVDVTSLRRSGSFPVDTQRFLRPDGTCTLCENALQEGTLQRLPLALTNWRAQGSGCQRHVTRAKVSSTEAVARDAARSIRNDWKVGLDVTPKPTSNVHVSVAGSHSQAANFAAQKTHQDQYSFSTDTVECRFYSFHVVHTPPLHPDFKRALGDLPHHFNASTQPAYLRLISNYGTHFIRAVELGGRISALTALRTCELALEGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACEEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLLDSQDPRREALRRALSQYLTDRARWRDCSRPCPPGRQKSPRDPCQCVCHGSAVTTQDCCPRQRGLAQLEVTFIQAWGLWGDWFTATDAYVKLFFGGQELRTSTVWDNNNPIWSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEVRCNLNHGHLKFRYHARCLPHLGGGTCLDYVPQMLLGEPPGNRSGAVW

Target ORF information:

RefSeq Version	NM_001083116.3
Organism	Homo sapiens(human)
Definition	Homo sapiens perforin 1 (PRF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083116.3

ORF Insert Sequence:

ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT 
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA 
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA 
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT 
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT 
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC 
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG 
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT 
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG 
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC 
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC 
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC 
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG 
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC 
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC 
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC 
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG 
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG 
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA 
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC 
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG 
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC 
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG 
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT 
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC 
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA 
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRF1 ( NM_001083116.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001076585.1 Homo sapiens perforin 1 (PRF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu23295
Clone ID Related Accession (Same CDS sequence)	NM_001083116.1 , NM_005041.5 , NM_005041.4 , NM_001083116.3 , NM_001083116.2
Accession Version	NM_001083116.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	perforin-1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY075867.1, DA941244.1, X13224.1, BC047695.1 and BQ654088.1. On Aug 31, 2017 this sequence version replaced NM_001083116.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279851.3847.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155751, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT 
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA 
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA 
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT 
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT 
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC 
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG 
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT 
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG 
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC 
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC 
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC 
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG 
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC 
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC 
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC 
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG 
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG 
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA 
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC 
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG 
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC 
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG 
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT 
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC 
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA 
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076585.1
CDS	175..1842
Translation	MAARLLLLGILLLLLPLPVPAPCHTAARSECKRSHKFVPGAWLAGEGVDVTSLRRSGSFPVDTQRFLRPDGTCTLCENALQEGTLQRLPLALTNWRAQGSGCQRHVTRAKVSSTEAVARDAARSIRNDWKVGLDVTPKPTSNVHVSVAGSHSQAANFAAQKTHQDQYSFSTDTVECRFYSFHVVHTPPLHPDFKRALGDLPHHFNASTQPAYLRLISNYGTHFIRAVELGGRISALTALRTCELALEGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACEEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLLDSQDPRREALRRALSQYLTDRARWRDCSRPCPPGRQKSPRDPCQCVCHGSAVTTQDCCPRQRGLAQLEVTFIQAWGLWGDWFTATDAYVKLFFGGQELRTSTVWDNNNPIWSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEVRCNLNHGHLKFRYHARCLPHLGGGTCLDYVPQMLLGEPPGNRSGAVW

Target ORF information:

RefSeq Version	NM_001083116.2
Organism	Homo sapiens(human)
Definition	Homo sapiens perforin 1 (PRF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083116.2

ORF Insert Sequence:

ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT 
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA 
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA 
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT 
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT 
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC 
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG 
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT 
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG 
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC 
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC 
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC 
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG 
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC 
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC 
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC 
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG 
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG 
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA 
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC 
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG 
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC 
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG 
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT 
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC 
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA 
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Perforin gene variation influences survival in childhood acute lymphoblastic leukemia.
Leukemia research6529-33(2018 Jan)
Jaworowska A,Pastorczak A,Trelinska J,Wypyszczak K,Borowiec M,Fendler W,Sedek L,Szczepanski T,Ploski R,M?ynarski W

Regulation of perforin activation and pre-synaptic toxicity through C-terminal glycosylation.
EMBO reports18(10)1775-1785(2017 Oct)
House IG,House CM,Brennan AJ,Gilan O,Dawson MA,Whisstock JC,Law RH,Trapani JA,Voskoboinik I

Finding a Balance between Protection and Pathology: The Dual Role of Perforin in Human Disease.
International journal of molecular sciences18(8)(2017 Jul)
Willenbring RC,Johnson AJ

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PRF1 cDNA ORF clone, Homo sapiens(human)

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ORF Insert Sequence:

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ORF Insert Sequence:

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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