Prf1 cDNA ORF clone, Mus musculus(house mouse)

The following Prf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu22740 NM_011073.3
Latest version!
Mus musculus perforin 1 (pore forming protein) (Prf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu22740 XM_006513370.4
Latest version!
Mus musculus perforin 1 (pore forming protein) (Prf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OMu22740 XM_006513370.3 Mus musculus perforin 1 (pore forming protein) (Prf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu22740
    Clone ID Related Accession (Same CDS sequence) XM_006513370.4 , XM_006513370.3 , NM_011073.3
    Accession Version NM_011073.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product perforin-1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC132300.1. On Mar 28, 2009 this sequence version replaced NM_011073.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC132300.1, M23182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCCACGT GCCTGTTCCT CCTGGGCCTT TTCCTGCTGC TGCCACGACC TGTCCCTGCT 
    CCCTGCTACA CTGCCACTCG GTCAGAATGC AAGCAGAAGC ACAAGTTCGT GCCAGGTGTA
    TGGATGGCTG GGGAAGGCAT GGATGTGACT ACCCTCCGCC GCTCCGGCTC CTTCCCAGTG
    AACACACAGA GGTTCCTGAG GCCTGACCGC ACCTGCACCC TCTGTAAAAA CTCCCTAATG
    AGAGACGCCA CACAGCGCCT ACCTGTGGCA ATCACCCACT GGCGGCCTCA CAGCTCACAC
    TGCCAGCGTA ATGTGGCCGC AGCCAAGGTC CACTCCACGG AGGGTGTGGC CCGGGAGGCA
    GCTGCTAATA TCAATAACGA CTGGCGTGTG GGGCTGGATG TGAACCCTAG GCCAGAGGCA
    AACATGCGCG CCTCCGTGGC TGGCTCCCAC TCCAAGGTAG CCAATTTTGC AGCTGAGAAG
    ACCTATCAGG ACCAGTACAA CTTTAATAGC GACACAGTAG AGTGTCGCAT GTACAGTTTT
    CGCCTGGTAC AAAAACCTCC ACTCCACCTT GACTTCAAAA AGGCGCTCAG AGCCCTCCCC
    CGCAACTTTA ACAGCTCCAC AGAGCATGCT TACCACAGGC TCATCTCCTC CTATGGCACG
    CACTTTATCA CGGCTGTGGA CCTCGGTGGC CGCATCTCGG TCCTTACAGC CCTGCGTACC
    TGTCAGCTGA CCCTGAATGG GCTCACAGCT GATGAGGTAG GAGACTGCCT GAACGTGGAG
    GCCCAGGTCA GCATCGGTGC CCAAGCCAGC GTCTCCAGTG AATACAAAGC TTGTGAGGAG
    AAGAAGAAAC AGCACAAAAT GGCCACCTCT TTCCACCAGA CCTACCGTGA GCGTCACGTC
    GAAGTACTTG GTGGCCCTCT GGACTCCACG CATGATCTGC TCTTCGGGAA CCAAGCTACA
    CCAGAGCAGT TCTCAACCTG GACAGCCTCA CTGCCCAGCA ACCCTGGTCT GGTGGACTAC
    AGCCTGGAGC CCCTGCACAC ATTACTGGAA GAACAGAACC CGAAGCGGGA GGCTCTGAGA
    CAGGCTATCA GCCATTATAT AATGAGCAGA GCCCGGTGGC AGAACTGTAG CAGGCCCTGC
    AGGTCAGGCC AGCATAAGAG TAGCCATGAT TCATGCCAGT GTGAGTGCCA GGATTCAAAG
    GTCACCAACC AGGACTGCTG CCCACGACAG AGGGGCTTGG CCCATTTGGT GGTAAGCAAT
    TTCCGGGCAG AACATCTGTG GGGAGACTAC ACCACAGCTA CTGATGCCTA CCTAAAGGTC
    TTCTTTGGTG GCCAGGAGTT CAGGACCGGT GTCGTGTGGA ACAATAACAA TCCCCGGTGG
    ACTGACAAGA TGGACTTTGA GAATGTGCTC CTGTCCACAG GGGGACCCCT CAGGGTGCAG
    GTCTGGGATG CCGACTACGG CTGGGATGAT GACCTTCTTG GTTCTTGTGA CAGGTCTCCC
    CACTCTGGTT TCCATGAGGT GACATGTGAG CTAAACCACG GCAGGGTGAA ATTCTCCTAC
    CATGCCAAGT GTCTGCCCCA TCTCACTGGA GGGACCTGCC TGGAGTATGC CCCCCAGGGG
    CTTCTGGGAG ATCCTCCAGG AAACCGCAGT GGGGCTGTGT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_035203.3
    CDS56..1720
    Translation

    Target ORF information:

    RefSeq Version NM_011073.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus perforin 1 (pore forming protein) (Prf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_011073.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCCACGT GCCTGTTCCT CCTGGGCCTT TTCCTGCTGC TGCCACGACC TGTCCCTGCT 
    CCCTGCTACA CTGCCACTCG GTCAGAATGC AAGCAGAAGC ACAAGTTCGT GCCAGGTGTA
    TGGATGGCTG GGGAAGGCAT GGATGTGACT ACCCTCCGCC GCTCCGGCTC CTTCCCAGTG
    AACACACAGA GGTTCCTGAG GCCTGACCGC ACCTGCACCC TCTGTAAAAA CTCCCTAATG
    AGAGACGCCA CACAGCGCCT ACCTGTGGCA ATCACCCACT GGCGGCCTCA CAGCTCACAC
    TGCCAGCGTA ATGTGGCCGC AGCCAAGGTC CACTCCACGG AGGGTGTGGC CCGGGAGGCA
    GCTGCTAATA TCAATAACGA CTGGCGTGTG GGGCTGGATG TGAACCCTAG GCCAGAGGCA
    AACATGCGCG CCTCCGTGGC TGGCTCCCAC TCCAAGGTAG CCAATTTTGC AGCTGAGAAG
    ACCTATCAGG ACCAGTACAA CTTTAATAGC GACACAGTAG AGTGTCGCAT GTACAGTTTT
    CGCCTGGTAC AAAAACCTCC ACTCCACCTT GACTTCAAAA AGGCGCTCAG AGCCCTCCCC
    CGCAACTTTA ACAGCTCCAC AGAGCATGCT TACCACAGGC TCATCTCCTC CTATGGCACG
    CACTTTATCA CGGCTGTGGA CCTCGGTGGC CGCATCTCGG TCCTTACAGC CCTGCGTACC
    TGTCAGCTGA CCCTGAATGG GCTCACAGCT GATGAGGTAG GAGACTGCCT GAACGTGGAG
    GCCCAGGTCA GCATCGGTGC CCAAGCCAGC GTCTCCAGTG AATACAAAGC TTGTGAGGAG
    AAGAAGAAAC AGCACAAAAT GGCCACCTCT TTCCACCAGA CCTACCGTGA GCGTCACGTC
    GAAGTACTTG GTGGCCCTCT GGACTCCACG CATGATCTGC TCTTCGGGAA CCAAGCTACA
    CCAGAGCAGT TCTCAACCTG GACAGCCTCA CTGCCCAGCA ACCCTGGTCT GGTGGACTAC
    AGCCTGGAGC CCCTGCACAC ATTACTGGAA GAACAGAACC CGAAGCGGGA GGCTCTGAGA
    CAGGCTATCA GCCATTATAT AATGAGCAGA GCCCGGTGGC AGAACTGTAG CAGGCCCTGC
    AGGTCAGGCC AGCATAAGAG TAGCCATGAT TCATGCCAGT GTGAGTGCCA GGATTCAAAG
    GTCACCAACC AGGACTGCTG CCCACGACAG AGGGGCTTGG CCCATTTGGT GGTAAGCAAT
    TTCCGGGCAG AACATCTGTG GGGAGACTAC ACCACAGCTA CTGATGCCTA CCTAAAGGTC
    TTCTTTGGTG GCCAGGAGTT CAGGACCGGT GTCGTGTGGA ACAATAACAA TCCCCGGTGG
    ACTGACAAGA TGGACTTTGA GAATGTGCTC CTGTCCACAG GGGGACCCCT CAGGGTGCAG
    GTCTGGGATG CCGACTACGG CTGGGATGAT GACCTTCTTG GTTCTTGTGA CAGGTCTCCC
    CACTCTGGTT TCCATGAGGT GACATGTGAG CTAAACCACG GCAGGGTGAA ATTCTCCTAC
    CATGCCAAGT GTCTGCCCCA TCTCACTGGA GGGACCTGCC TGGAGTATGC CCCCCAGGGG
    CTTCTGGGAG ATCCTCCAGG AAACCGCAGT GGGGCTGTGT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22740
    Clone ID Related Accession (Same CDS sequence) XM_006513370.4 , XM_006513370.3 , NM_011073.3
    Accession Version XM_006513370.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product perforin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513370.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCCACGT GCCTGTTCCT CCTGGGCCTT TTCCTGCTGC TGCCACGACC TGTCCCTGCT 
    CCCTGCTACA CTGCCACTCG GTCAGAATGC AAGCAGAAGC ACAAGTTCGT GCCAGGTGTA
    TGGATGGCTG GGGAAGGCAT GGATGTGACT ACCCTCCGCC GCTCCGGCTC CTTCCCAGTG
    AACACACAGA GGTTCCTGAG GCCTGACCGC ACCTGCACCC TCTGTAAAAA CTCCCTAATG
    AGAGACGCCA CACAGCGCCT ACCTGTGGCA ATCACCCACT GGCGGCCTCA CAGCTCACAC
    TGCCAGCGTA ATGTGGCCGC AGCCAAGGTC CACTCCACGG AGGGTGTGGC CCGGGAGGCA
    GCTGCTAATA TCAATAACGA CTGGCGTGTG GGGCTGGATG TGAACCCTAG GCCAGAGGCA
    AACATGCGCG CCTCCGTGGC TGGCTCCCAC TCCAAGGTAG CCAATTTTGC AGCTGAGAAG
    ACCTATCAGG ACCAGTACAA CTTTAATAGC GACACAGTAG AGTGTCGCAT GTACAGTTTT
    CGCCTGGTAC AAAAACCTCC ACTCCACCTT GACTTCAAAA AGGCGCTCAG AGCCCTCCCC
    CGCAACTTTA ACAGCTCCAC AGAGCATGCT TACCACAGGC TCATCTCCTC CTATGGCACG
    CACTTTATCA CGGCTGTGGA CCTCGGTGGC CGCATCTCGG TCCTTACAGC CCTGCGTACC
    TGTCAGCTGA CCCTGAATGG GCTCACAGCT GATGAGGTAG GAGACTGCCT GAACGTGGAG
    GCCCAGGTCA GCATCGGTGC CCAAGCCAGC GTCTCCAGTG AATACAAAGC TTGTGAGGAG
    AAGAAGAAAC AGCACAAAAT GGCCACCTCT TTCCACCAGA CCTACCGTGA GCGTCACGTC
    GAAGTACTTG GTGGCCCTCT GGACTCCACG CATGATCTGC TCTTCGGGAA CCAAGCTACA
    CCAGAGCAGT TCTCAACCTG GACAGCCTCA CTGCCCAGCA ACCCTGGTCT GGTGGACTAC
    AGCCTGGAGC CCCTGCACAC ATTACTGGAA GAACAGAACC CGAAGCGGGA GGCTCTGAGA
    CAGGCTATCA GCCATTATAT AATGAGCAGA GCCCGGTGGC AGAACTGTAG CAGGCCCTGC
    AGGTCAGGCC AGCATAAGAG TAGCCATGAT TCATGCCAGT GTGAGTGCCA GGATTCAAAG
    GTCACCAACC AGGACTGCTG CCCACGACAG AGGGGCTTGG CCCATTTGGT GGTAAGCAAT
    TTCCGGGCAG AACATCTGTG GGGAGACTAC ACCACAGCTA CTGATGCCTA CCTAAAGGTC
    TTCTTTGGTG GCCAGGAGTT CAGGACCGGT GTCGTGTGGA ACAATAACAA TCCCCGGTGG
    ACTGACAAGA TGGACTTTGA GAATGTGCTC CTGTCCACAG GGGGACCCCT CAGGGTGCAG
    GTCTGGGATG CCGACTACGG CTGGGATGAT GACCTTCTTG GTTCTTGTGA CAGGTCTCCC
    CACTCTGGTT TCCATGAGGT GACATGTGAG CTAAACCACG GCAGGGTGAA ATTCTCCTAC
    CATGCCAAGT GTCTGCCCCA TCTCACTGGA GGGACCTGCC TGGAGTATGC CCCCCAGGGG
    CTTCTGGGAG ATCCTCCAGG AAACCGCAGT GGGGCTGTGT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006513433.1
    CDS453..2117
    Translation

    Target ORF information:

    RefSeq Version XM_006513370.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus perforin 1 (pore forming protein) (Prf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006513370.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCCACGT GCCTGTTCCT CCTGGGCCTT TTCCTGCTGC TGCCACGACC TGTCCCTGCT 
    CCCTGCTACA CTGCCACTCG GTCAGAATGC AAGCAGAAGC ACAAGTTCGT GCCAGGTGTA
    TGGATGGCTG GGGAAGGCAT GGATGTGACT ACCCTCCGCC GCTCCGGCTC CTTCCCAGTG
    AACACACAGA GGTTCCTGAG GCCTGACCGC ACCTGCACCC TCTGTAAAAA CTCCCTAATG
    AGAGACGCCA CACAGCGCCT ACCTGTGGCA ATCACCCACT GGCGGCCTCA CAGCTCACAC
    TGCCAGCGTA ATGTGGCCGC AGCCAAGGTC CACTCCACGG AGGGTGTGGC CCGGGAGGCA
    GCTGCTAATA TCAATAACGA CTGGCGTGTG GGGCTGGATG TGAACCCTAG GCCAGAGGCA
    AACATGCGCG CCTCCGTGGC TGGCTCCCAC TCCAAGGTAG CCAATTTTGC AGCTGAGAAG
    ACCTATCAGG ACCAGTACAA CTTTAATAGC GACACAGTAG AGTGTCGCAT GTACAGTTTT
    CGCCTGGTAC AAAAACCTCC ACTCCACCTT GACTTCAAAA AGGCGCTCAG AGCCCTCCCC
    CGCAACTTTA ACAGCTCCAC AGAGCATGCT TACCACAGGC TCATCTCCTC CTATGGCACG
    CACTTTATCA CGGCTGTGGA CCTCGGTGGC CGCATCTCGG TCCTTACAGC CCTGCGTACC
    TGTCAGCTGA CCCTGAATGG GCTCACAGCT GATGAGGTAG GAGACTGCCT GAACGTGGAG
    GCCCAGGTCA GCATCGGTGC CCAAGCCAGC GTCTCCAGTG AATACAAAGC TTGTGAGGAG
    AAGAAGAAAC AGCACAAAAT GGCCACCTCT TTCCACCAGA CCTACCGTGA GCGTCACGTC
    GAAGTACTTG GTGGCCCTCT GGACTCCACG CATGATCTGC TCTTCGGGAA CCAAGCTACA
    CCAGAGCAGT TCTCAACCTG GACAGCCTCA CTGCCCAGCA ACCCTGGTCT GGTGGACTAC
    AGCCTGGAGC CCCTGCACAC ATTACTGGAA GAACAGAACC CGAAGCGGGA GGCTCTGAGA
    CAGGCTATCA GCCATTATAT AATGAGCAGA GCCCGGTGGC AGAACTGTAG CAGGCCCTGC
    AGGTCAGGCC AGCATAAGAG TAGCCATGAT TCATGCCAGT GTGAGTGCCA GGATTCAAAG
    GTCACCAACC AGGACTGCTG CCCACGACAG AGGGGCTTGG CCCATTTGGT GGTAAGCAAT
    TTCCGGGCAG AACATCTGTG GGGAGACTAC ACCACAGCTA CTGATGCCTA CCTAAAGGTC
    TTCTTTGGTG GCCAGGAGTT CAGGACCGGT GTCGTGTGGA ACAATAACAA TCCCCGGTGG
    ACTGACAAGA TGGACTTTGA GAATGTGCTC CTGTCCACAG GGGGACCCCT CAGGGTGCAG
    GTCTGGGATG CCGACTACGG CTGGGATGAT GACCTTCTTG GTTCTTGTGA CAGGTCTCCC
    CACTCTGGTT TCCATGAGGT GACATGTGAG CTAAACCACG GCAGGGTGAA ATTCTCCTAC
    CATGCCAAGT GTCTGCCCCA TCTCACTGGA GGGACCTGCC TGGAGTATGC CCCCCAGGGG
    CTTCTGGGAG ATCCTCCAGG AAACCGCAGT GGGGCTGTGT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22740
    Clone ID Related Accession (Same CDS sequence) XM_006513370.4 , XM_006513370.3 , NM_011073.3
    Accession Version XM_006513370.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product perforin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513370.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCCACGT GCCTGTTCCT CCTGGGCCTT TTCCTGCTGC TGCCACGACC TGTCCCTGCT 
    CCCTGCTACA CTGCCACTCG GTCAGAATGC AAGCAGAAGC ACAAGTTCGT GCCAGGTGTA
    TGGATGGCTG GGGAAGGCAT GGATGTGACT ACCCTCCGCC GCTCCGGCTC CTTCCCAGTG
    AACACACAGA GGTTCCTGAG GCCTGACCGC ACCTGCACCC TCTGTAAAAA CTCCCTAATG
    AGAGACGCCA CACAGCGCCT ACCTGTGGCA ATCACCCACT GGCGGCCTCA CAGCTCACAC
    TGCCAGCGTA ATGTGGCCGC AGCCAAGGTC CACTCCACGG AGGGTGTGGC CCGGGAGGCA
    GCTGCTAATA TCAATAACGA CTGGCGTGTG GGGCTGGATG TGAACCCTAG GCCAGAGGCA
    AACATGCGCG CCTCCGTGGC TGGCTCCCAC TCCAAGGTAG CCAATTTTGC AGCTGAGAAG
    ACCTATCAGG ACCAGTACAA CTTTAATAGC GACACAGTAG AGTGTCGCAT GTACAGTTTT
    CGCCTGGTAC AAAAACCTCC ACTCCACCTT GACTTCAAAA AGGCGCTCAG AGCCCTCCCC
    CGCAACTTTA ACAGCTCCAC AGAGCATGCT TACCACAGGC TCATCTCCTC CTATGGCACG
    CACTTTATCA CGGCTGTGGA CCTCGGTGGC CGCATCTCGG TCCTTACAGC CCTGCGTACC
    TGTCAGCTGA CCCTGAATGG GCTCACAGCT GATGAGGTAG GAGACTGCCT GAACGTGGAG
    GCCCAGGTCA GCATCGGTGC CCAAGCCAGC GTCTCCAGTG AATACAAAGC TTGTGAGGAG
    AAGAAGAAAC AGCACAAAAT GGCCACCTCT TTCCACCAGA CCTACCGTGA GCGTCACGTC
    GAAGTACTTG GTGGCCCTCT GGACTCCACG CATGATCTGC TCTTCGGGAA CCAAGCTACA
    CCAGAGCAGT TCTCAACCTG GACAGCCTCA CTGCCCAGCA ACCCTGGTCT GGTGGACTAC
    AGCCTGGAGC CCCTGCACAC ATTACTGGAA GAACAGAACC CGAAGCGGGA GGCTCTGAGA
    CAGGCTATCA GCCATTATAT AATGAGCAGA GCCCGGTGGC AGAACTGTAG CAGGCCCTGC
    AGGTCAGGCC AGCATAAGAG TAGCCATGAT TCATGCCAGT GTGAGTGCCA GGATTCAAAG
    GTCACCAACC AGGACTGCTG CCCACGACAG AGGGGCTTGG CCCATTTGGT GGTAAGCAAT
    TTCCGGGCAG AACATCTGTG GGGAGACTAC ACCACAGCTA CTGATGCCTA CCTAAAGGTC
    TTCTTTGGTG GCCAGGAGTT CAGGACCGGT GTCGTGTGGA ACAATAACAA TCCCCGGTGG
    ACTGACAAGA TGGACTTTGA GAATGTGCTC CTGTCCACAG GGGGACCCCT CAGGGTGCAG
    GTCTGGGATG CCGACTACGG CTGGGATGAT GACCTTCTTG GTTCTTGTGA CAGGTCTCCC
    CACTCTGGTT TCCATGAGGT GACATGTGAG CTAAACCACG GCAGGGTGAA ATTCTCCTAC
    CATGCCAAGT GTCTGCCCCA TCTCACTGGA GGGACCTGCC TGGAGTATGC CCCCCAGGGG
    CTTCTGGGAG ATCCTCCAGG AAACCGCAGT GGGGCTGTGT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006513433.1
    CDS248..1912
    Translation

    Target ORF information:

    RefSeq Version XM_006513370.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus perforin 1 (pore forming protein) (Prf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006513370.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCCACGT GCCTGTTCCT CCTGGGCCTT TTCCTGCTGC TGCCACGACC TGTCCCTGCT 
    CCCTGCTACA CTGCCACTCG GTCAGAATGC AAGCAGAAGC ACAAGTTCGT GCCAGGTGTA
    TGGATGGCTG GGGAAGGCAT GGATGTGACT ACCCTCCGCC GCTCCGGCTC CTTCCCAGTG
    AACACACAGA GGTTCCTGAG GCCTGACCGC ACCTGCACCC TCTGTAAAAA CTCCCTAATG
    AGAGACGCCA CACAGCGCCT ACCTGTGGCA ATCACCCACT GGCGGCCTCA CAGCTCACAC
    TGCCAGCGTA ATGTGGCCGC AGCCAAGGTC CACTCCACGG AGGGTGTGGC CCGGGAGGCA
    GCTGCTAATA TCAATAACGA CTGGCGTGTG GGGCTGGATG TGAACCCTAG GCCAGAGGCA
    AACATGCGCG CCTCCGTGGC TGGCTCCCAC TCCAAGGTAG CCAATTTTGC AGCTGAGAAG
    ACCTATCAGG ACCAGTACAA CTTTAATAGC GACACAGTAG AGTGTCGCAT GTACAGTTTT
    CGCCTGGTAC AAAAACCTCC ACTCCACCTT GACTTCAAAA AGGCGCTCAG AGCCCTCCCC
    CGCAACTTTA ACAGCTCCAC AGAGCATGCT TACCACAGGC TCATCTCCTC CTATGGCACG
    CACTTTATCA CGGCTGTGGA CCTCGGTGGC CGCATCTCGG TCCTTACAGC CCTGCGTACC
    TGTCAGCTGA CCCTGAATGG GCTCACAGCT GATGAGGTAG GAGACTGCCT GAACGTGGAG
    GCCCAGGTCA GCATCGGTGC CCAAGCCAGC GTCTCCAGTG AATACAAAGC TTGTGAGGAG
    AAGAAGAAAC AGCACAAAAT GGCCACCTCT TTCCACCAGA CCTACCGTGA GCGTCACGTC
    GAAGTACTTG GTGGCCCTCT GGACTCCACG CATGATCTGC TCTTCGGGAA CCAAGCTACA
    CCAGAGCAGT TCTCAACCTG GACAGCCTCA CTGCCCAGCA ACCCTGGTCT GGTGGACTAC
    AGCCTGGAGC CCCTGCACAC ATTACTGGAA GAACAGAACC CGAAGCGGGA GGCTCTGAGA
    CAGGCTATCA GCCATTATAT AATGAGCAGA GCCCGGTGGC AGAACTGTAG CAGGCCCTGC
    AGGTCAGGCC AGCATAAGAG TAGCCATGAT TCATGCCAGT GTGAGTGCCA GGATTCAAAG
    GTCACCAACC AGGACTGCTG CCCACGACAG AGGGGCTTGG CCCATTTGGT GGTAAGCAAT
    TTCCGGGCAG AACATCTGTG GGGAGACTAC ACCACAGCTA CTGATGCCTA CCTAAAGGTC
    TTCTTTGGTG GCCAGGAGTT CAGGACCGGT GTCGTGTGGA ACAATAACAA TCCCCGGTGG
    ACTGACAAGA TGGACTTTGA GAATGTGCTC CTGTCCACAG GGGGACCCCT CAGGGTGCAG
    GTCTGGGATG CCGACTACGG CTGGGATGAT GACCTTCTTG GTTCTTGTGA CAGGTCTCCC
    CACTCTGGTT TCCATGAGGT GACATGTGAG CTAAACCACG GCAGGGTGAA ATTCTCCTAC
    CATGCCAAGT GTCTGCCCCA TCTCACTGGA GGGACCTGCC TGGAGTATGC CCCCCAGGGG
    CTTCTGGGAG ATCCTCCAGG AAACCGCAGT GGGGCTGTGT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.