Kif20b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kif20b
Entrez Gene ID	309523
Full Name	kinesin family member 20B
Synonyms	Mphosph1
General protein information	Preferred Names kinesin family member 20B Names kinesin-like protein KIF20B M-phase phosphoprotein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q53

mRNA and Protein(s)

mRNA	Protein	Name
XM_006231285.2	XP_006231347.1	kinesin-like protein KIF20B isoform X5
NM_001107609.2	NP_001101079.2	kinesin-like protein KIF20B
XM_017589297.1	XP_017444786.1	kinesin-like protein KIF20B isoform X1
XM_008760329.2	XP_008758551.1	kinesin-like protein KIF20B isoform X2
XM_006231286.1	XP_006231348.1	kinesin-like protein KIF20B isoform X6
XM_006231281.2	XP_006231343.1	kinesin-like protein KIF20B isoform X1
XM_006231284.2	XP_006231346.1	kinesin-like protein KIF20B isoform X4
XM_006231283.2	XP_006231345.1	kinesin-like protein KIF20B isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KIF20B	NP_001098728.1
Bos taurus (cattle)	LOC782598	XP_005222161.1
Mus musculus (house mouse)	Kif20b	NP_898867.1
Pan troglodytes (chimpanzee)	KIF20B	XP_507908.2
Rattus norvegicus (Norway rat)	Kif20b	NP_001101079.2
Homo sapiens (human)	KIF20B	NP_057279.2
Danio rerio (zebrafish)	kif20bb	XP_003199630.2
Canis lupus familiaris (dog)	KIF20B	XP_534957.4
Xenopus tropicalis (tropical clawed frog)	kif20b	XP_004915944.1
	LOC696152	XP_002808551.1

Gene Ontology
Bibliography

Related articles in PubMed

The vertebrate-specific Kinesin-6, Kif20b, is required for normal cytokinesis of polarized cortical stem cells and cerebral cortex size.
Janisch KM, Vock VM, Fleming MS, Shrestha A, Grimsley-Myers CM, Rasoul BA, Neale SA, Cupp TD, Kinchen JM, Liem KF Jr, Dwyer ND
Development (Cambridge, England)140(23)4672-82(2013 Dec)

Shootin1 acts in concert with KIF20B to promote polarization of migrating neurons.
Sapir T, Levy T, Sakakibara A, Rabinkov A, Miyata T, Reiner O
The Journal of neuroscience : the official journal of the Society for Neuroscience33(29)11932-48(2013 Jul)

Endoglin haploinsufficiency promotes fibroblast accumulation during wound healing through Akt activation.
Pericacho M, Velasco S, Prieto M, Llano E, L?pez-Novoa JM, Rodr?guez-Barbero A
PloS one8(1)e54687(2013)

Oncogenic role of MPHOSPH1, a cancer-testis antigen specific to human bladder cancer.
Kanehira M, Katagiri T, Shimo A, Takata R, Shuin T, Miki T, Fujioka T, Nakamura Y
Cancer research67(7)3276-85(2007 Apr)

Analysis of mouse embryonic patterning and morphogenesis by forward genetics.
Garc?a-Garc?a MJ, Eggenschwiler JT, Caspary T, Alcorn HL, Wyler MR, Huangfu D, Rakeman AS, Lee JD, Feinberg EH, Timmer JR, Anderson KV
Proceedings of the National Academy of Sciences of the United States of America102(17)5913-9(2005 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Kif20b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kif20b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa41327	NM_001107609.2 Latest version!	Rattus norvegicus kinesin family member 20B (Kif20b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa24035	XM_006231281.2 Latest version!	Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa24035	XM_017589297.1 Latest version!	Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa24036	XM_006231283.2 Latest version!	Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa24037	XM_006231284.2 Latest version!	Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa24038	XM_006231285.2 Latest version!	Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa24039	XM_006231286.1 Latest version!	Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa46765	XM_008760329.2 Latest version!	Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kif20b ( NM_001107609.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101079.2 Rattus norvegicus kinesin family member 20B (Kif20b), mRNA.

Price & Availability↑

CloneID	ORa41327
Clone ID Related Accession (Same CDS sequence)	NM_001107609.2
Accession Version	NM_001107609.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF20B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC096809.11. On Jan 9, 2008 this sequence version replaced NM_001107609.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGAATCTC ATTTGAATCA AGACGGAGTG CCTAGACCAT CTTATGTTTT TAGTGCTGAC 
CCAATTGCAA GGCCTTCGGA AATCAATTTT GATGGCGTTA AGCTTGATCT CTCTCATGAG 
TTTTCCTTAG TTGCCGCAAA CCCTGGGGCA AACAGTTTGG AATCTAAAAA TTACCTCCAG 
GTTTGTCTTC GAATAAGACC ATTTACACAG TCGGAAAAAG GACACGAGGC TGAGGGCTGT 
GTGCAAGTTC TGGATTCACA GACTGTTCTG CTGAAAGACC CTCAAAGCAT CCTTGGCCAC 
TTAAGTGAGA AGAGCTCCGG ACAGATGGCA CAGAAATTCA GCTTTTCTAG GGTTTTTGGA 
CCCGAAACTT CACAGAAAGA GTTCTTTCAG GGTTGCATTA TGCAGCCAGT AAAAGACCTC 
TTAAAAGGGC ACAGTCGACT GATCTTCACA TATGGGCTGA CCAATTCTGG AAAAACATAT 
ACATTTCAAG GCACAGAAGA AAACATTGGC ATTCTGCCTA GAACACTGAA TGTATTGTTT 
GATAGTCTTC AGGAAAGACT CTATACAAAG ATGAGCTTCA AACCACATAG ATGTAGAGAA 
TACCTACAGT TGTCATCTGA TCAAGAGAAA GAGGAAAGTG CTAACAAAAA TACATTGCTG 
CGACAAATTA AAGAGGTTAC CATACATAAT GACAGTTATG ACATTCTTTG TGGACGCTTA 
ACAAACTCTT TGACTATACC AGAATTTGAA GAAACCATGA ACAATTGTGA ACAATCTAGT 
CTCAATGTGG ATAATATAAA ATATTCAGTG TGGGTTTCTT TCTTTGAGAT TTACAATGAA 
AGTATTTATG ATTTGTTTGT TCCTGTGTCC TCTAAGTTGC AAAAGAGAAA AATGCTACGC 
CTTTCCCAAG ATGTAAAGGG CTATTCTTTT ATAAAAGATC TGCAGTGGAT CCAGGTATCT 
GATTCTAAAG AAGCATATAG ACTCTTAAAA CTAGGAGTGA AGCATCAGAG TGTTGCCTTC 
ACAAAACTGA ACAATGCTTC TAGCAGAAGT CACAGCATAT TCACTATTAG AATATTACAG 
ATAGAAGACT CGGAAATACC TCGTGTAACA CGAGTTAGTG AATTGTCGCT ATGTGATCTC 
GCTGGTTCAG AACGAAGCAT GAAGACGCAA AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG 
AATATCAACA CTTCTTTACT GACTCTGGGA AAGTGCATCA GTGTTCTGAA GAACAGTGAC 
AAGTCCAAGA TTCAACAACA TGTGCCTTTT CGAGAAAGTA AACTGACCCA CTATTTCCAA 
AGCTTTTTTA CTGGAAAGGG GAAAATATGT ATGATCATCA ATATTAGTCA GTGTTGTTCT 
GCCTATGATG AGACACTCAA TGTACTGAAG TTCTCAACTG TTGCACAAAA GGTCTATGTT 
CCAGATACTT TAAGTTCTTC TCAAGAGAAA TCGTTTGGGT CTACCAAATC TTTACAAGAT 
GTATCCCTAG GCAGTAATTT GGATAATAAA ATACTAAATG TAAAAAGAAA AACTGTTTCA 
TGGGAAAATA GTCTGGAAGA TGTGGTTGAA AATGAAGATT TGGTCGAGGA CTTAGAAGAA 
AATGAAGAAA CACAAAACAT GGAAACTGAA CTTACAGATG AAGATAGTGA TAAGCCGTTA 
GAGGAAGGCG GAGTCTGCGC AGGTCACGGG AAGAACAAGA AACTGTTGGA TTTAATAGAA 
AACTTGAAAA AAAGACTAAT AAATGAAAAA AAAGAAAAGT TAACATTGGA ATTTAAAATC 
CGAGAAGAAG TGACACAGGA ATTCACTCAG TATTGGAGCC AAAGGGAAGC TGATTTTAAG 
GAAACACTTC TTCATGAACG GGAGATATTA GAAGAAAATG CTGAACGTCG CTTAGCAATT 
TTCAAAGATT TGGTTGGAAA ACCTGGTGAG AGTCAAGATG AACCAGCAAG CAGATTTTGT 
ACCATGGAAC TTGAAACTGA AGAGGCTATT GCTTGTTTGC AGCTAAAGTA TAATCAAGTT 
AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA TTAATTAAAG CCCAAGAAGA GCTAAAAAAT 
AAAGAGAGTG ACTCTCTGGT TCAGGCACTC AAGACATCAA GTAAGAGCTT GTTAACTTCA 
GGTCAGATAG TTACCAAGCT GGTTTCAGTA CTTGGAGGTG AAGGCGGGAG AATCAATAAT 
ATCCAGGATA TAAAGCAGGA AAATACCGTA ACATGTAAAG TTGATACATC TTTAATCAGC 
AATAAGTCCA CTGGTAATGA AACAACTGAA ATGCCCAAGA AGAGTAGAAC CCAGACACAT 
TCAGAAAGGA AAAGATTAAA TGAAGATGGA CTTCAGCTTG GTGAACCACC AGCAAAGAAA 
GGGCTTATAC TTATTAGTCC ACCCATCACT GAAGACCAAG ACAAAAGGGA AGAAATGCAA 
CAGTCTGTTT CAGAAGGTGC AGAAGAAGAC AGTAGAGTTT TGCAGGAAAA GAATGAAGAG 
CTAAAAAGAC TTTTAACTAT TGGTGAGAAT GAACTCAGAA ATGCAAAGGA GGAAAAGGCA 
GAATTAAATA AACAGGTTGT GAGTTTGCAG CAGCAACTTT GTTTCTTTGA AGAAAAGAAC 
TCATCCCTGA GGGCAGAGGT AGAGCAAATC CAGGCCAGTT ATGACCTTGC AGCTGCTGAG 
CTGCATACTC AGAGAGCTGT GAACCAGGAG CAGAAGGACA GGATCCTGCA ATTGTCAGGG 
AAGATGGAAA CTGCGGCAAG ACGTATTGAA AGCAATGTTT CACAGATAAA GCAAATGCAA 
ACAAAAATAG ATGAACTGCG ATCTCTTGAT TCACCTTCTC ATATTTCAAA AATAGATTTA 
CTCAATCTCC AGGATCTATC AAGTGGTGCT AATTTGCTGA ACACATCACA ACAGCTTCCA 
GGTAGTGACC TACCGAGCAC ATGGGTTAAA GAGTTTCATA CTCAGGAGCT CAGCAGGGAA 
AGTTCCTTCC ACTCTAGCAT TGAAGCCATC TGGGAAGAAT GTAAAGAGAT TGTGAAGGCT 
TCCTCCAAAA AGTCTCATCA GATTCAGGGA CTAGAAGAAC TAATTGAAAA ATTACAGGTG 
GAAGTAAAAA ACTGCAGGGA TGAGAACAGT GAGCTTCGAG CAAAGGAGAG TGAGGATAAG 
AATCGAGACC AGCAGCTGAA GGAGAAAGAA AGTCTAATCC AGCAGCTTAG AGAAGAACTT 
CAGGAAACCA CTGTCAGTCT CCGTGTCCAA GTACAGCTAG TAGCGGAAAG AGAGCAAGCT 
CTTTCAGAGC TTTCCCGGGA TGTTACCTGC TATAAGGCAA AAGTAAAGGA CCTTGAAGTG 
ATGGTAGAGA CCCAGAAAGA GGAATGTAAG CGCTTAGCTG AGTTAGAGCA GAGCATTTTA 
GAGAAGGAAT CTGCCATCCT AAAGCTGGAA GCGAGTCTAA AGGAATTGGA AGCAAAGCAC 
CAGGATCACA TCAGAAGTAC CACACATTTA AATGCAGAGG AAGTCAAGTT CAGAGAAGAA 
ATTACACAGT TGGCGAATAA TTTGCATGAT ACAAAACAGT TACTTCAGTC AAAGGAAGAA 
GAAAATGAAA TTAGCAGACA AGAGACAGAA AAGTTGAAAG AGGAGCTTGC TGCAAACTCT 
GTTCTTACCC AGAATCTCCA AGCAGACCTT CAGCGGAAGG AGGAAGATTG TGCTGAGCTA 
AAGGAGAAGT TCACTGATGC CAAAAAGCAG ATCGAGCAGG TACAGAGGGA GGTATCTGTA 
ATGCGTGATG AAGAGAAGTC GTTGAGGACT AAAATTAATG AACTGGAGAA AAAGAAAAAC 
CAATATTCTC AGGAAATAGA TATGAAACAA CGAACCATTC AGCAACTTAA GGAGCAGTTA 
AGTAATCAGA AAATGGAAGA AGTTGTACAA CAGTATGAGA AAGTGTGCAA AGATCTCAGC 
GTTAAGGAGA AGCTGATTGA AGCTATGCGA CTGACACTAG TGGAGCAGGA GCAAACTCAG 
GCAGAGCAGG ACAGGATGCT TGAGGCTAAG TCACAGGAAG CTGACTGGCT AGCCGGAGAA 
TTGGATACAT GGAAAGACAA ATTCAAAGAT CTGGAGACTA GAAGTAATCA AAAGGTAACT 
ACAGAGGCCA TGGAGGACAG CGACGTGCTT AGTGAGAAGT TCAGGAAGCT TCAGGACGAG 
CTGCAGGAAT CTGAAGAGAA ACATAAAGCT GATAGAAAGA AGTGGTTAGA AGAAAAAGCG 
GTTCTTACAA CTCAAGCAAA AGAAGCAGAG ACTCTACGAA ACAGGGAGAT GAAAAAATAT 
GCTGAGGACA GGGAGCGTTG TCTGAAGCTG CAGAATGAAG TGGAAACACT GACAGCACAG 
CTGGCGGAGA AAACTGGTGA GCTTCAGAAG TGGAGAGAAG AGCGGGACCA GCTGGTCACA 
GCTGTGGAGA CACAGATGCA GGCTCTGCTC TCCAGCAGCA AGCATAAGGA CGAAGAGATC 
CAGCAACTGA GAAAGGCCGT GGCAAAGAGC ACGGGGACAG AAAACCAGAC CATGAATCTC 
AAGCCTGAAT GTAATGATTC AGTCGATCTT GGTGGAGTTG AAACTGAACT TCAGTCAACA 
AGTTTTGAAA TTTCTAGGAA TACAGCAGAG GATGGATCTG TAGTTCTTGA CTCTTGTGAA 
GTGTCAACAG AAAATGTACA AAGTACTCGA TTTCCAAAGC CTGAACTGGA GATTCAGTTT 
ACACCTCTGC AGCCAAACAA AGTGGCAGTG AAGCACCCTG GTTGTGCCAC ACCAGTCACA 
ATTAAGATCC CCAAGGCACG GAAGAGGAAG AGTGGCGAGG TGGAGGAGGA CTTGGTGAAA 
TGTGAAAATA AGAAGAATTC CACACCCAGA AGTAATGTGA AGTTTCCCGT TTCAGAGCAT 
AGAAATTCCT CTTTCAAAAA GGAACAAAAG GTTTTCTTAT GTCCGTCATC TAAGAAGACA 
TACTCCTTAC GGAGCCAAGC ATCTACGGTT GGTGCGAATG TAGCCTCTAA AAAAAAAGAG 
GGAACACTAC AGAAATTTGG AGACTTCTTA CAACATTCTC CAACAATCCT TCAATCAAAA 
GCAAAAAAGA TAATTGAAAC AATGAGTTCC CCAAAGTTAT CAACTGTAGA AGTAAGTAAA 
GAAAATGTAT CCCAGCCTAA AAAAGCCAAA CGGAAATTGT ACAGAAATGA AATTTCATCT 
CCCATCAACA TATCTGGCCA AGTGATTTTA ATGGAACAGA AAGCAAAAGA AACTGATCAT 
CAGATTCTCA AACGACGGCT TCGAACACGA ACAGCCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101079.2
CDS	68..5389
Translation	MESHLNQDGVPRPSYVFSADPIARPSEINFDGVKLDLSHEFSLVAANPGANSLESKNYLQVCLRIRPFTQSEKGHEAEGCVQVLDSQTVLLKDPQSILGHLSEKSSGQMAQKFSFSRVFGPETSQKEFFQGCIMQPVKDLLKGHSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLFDSLQERLYTKMSFKPHRCREYLQLSSDQEKEESANKNTLLRQIKEVTIHNDSYDILCGRLTNSLTIPEFEETMNNCEQSSLNVDNIKYSVWVSFFEIYNESIYDLFVPVSSKLQKRKMLRLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGVKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPRVTRVSELSLCDLAGSERSMKTQSEGERLREAGNINTSLLTLGKCISVLKNSDKSKIQQHVPFRESKLTHYFQSFFTGKGKICMIINISQCCSAYDETLNVLKFSTVAQKVYVPDTLSSSQEKSFGSTKSLQDVSLGSNLDNKILNVKRKTVSWENSLEDVVENEDLVEDLEENEETQNMETELTDEDSDKPLEEGGVCAGHGKNKKLLDLIENLKKRLINEKKEKLTLEFKIREEVTQEFTQYWSQREADFKETLLHEREILEENAERRLAIFKDLVGKPGESQDEPASRFCTMELETEEAIACLQLKYNQVKAELAETKEELIKAQEELKNKESDSLVQALKTSSKSLLTSGQIVTKLVSVLGGEGGRINNIQDIKQENTVTCKVDTSLISNKSTGNETTEMPKKSRTQTHSERKRLNEDGLQLGEPPAKKGLILISPPITEDQDKREEMQQSVSEGAEEDSRVLQEKNEELKRLLTIGENELRNAKEEKAELNKQVVSLQQQLCFFEEKNSSLRAEVEQIQASYDLAAAELHTQRAVNQEQKDRILQLSGKMETAARRIESNVSQIKQMQTKIDELRSLDSPSHISKIDLLNLQDLSSGANLLNTSQQLPGSDLPSTWVKEFHTQELSRESSFHSSIEAIWEECKEIVKASSKKSHQIQGLEELIEKLQVEVKNCRDENSELRAKESEDKNRDQQLKEKESLIQQLREELQETTVSLRVQVQLVAEREQALSELSRDVTCYKAKVKDLEVMVETQKEECKRLAELEQSILEKESAILKLEASLKELEAKHQDHIRSTTHLNAEEVKFREEITQLANNLHDTKQLLQSKEEENEISRQETEKLKEELAANSVLTQNLQADLQRKEEDCAELKEKFTDAKKQIEQVQREVSVMRDEEKSLRTKINELEKKKNQYSQEIDMKQRTIQQLKEQLSNQKMEEVVQQYEKVCKDLSVKEKLIEAMRLTLVEQEQTQAEQDRMLEAKSQEADWLAGELDTWKDKFKDLETRSNQKVTTEAMEDSDVLSEKFRKLQDELQESEEKHKADRKKWLEEKAVLTTQAKEAETLRNREMKKYAEDRERCLKLQNEVETLTAQLAEKTGELQKWREERDQLVTAVETQMQALLSSSKHKDEEIQQLRKAVAKSTGTENQTMNLKPECNDSVDLGGVETELQSTSFEISRNTAEDGSVVLDSCEVSTENVQSTRFPKPELEIQFTPLQPNKVAVKHPGCATPVTIKIPKARKRKSGEVEEDLVKCENKKNSTPRSNVKFPVSEHRNSSFKKEQKVFLCPSSKKTYSLRSQASTVGANVASKKKEGTLQKFGDFLQHSPTILQSKAKKIIETMSSPKLSTVEVSKENVSQPKKAKRKLYRNEISSPINISGQVILMEQKAKETDHQILKRRLRTRTAK

Target ORF information:

RefSeq Version	NM_001107609.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 20B (Kif20b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107609.2

ORF Insert Sequence:

ATGGAATCTC ATTTGAATCA AGACGGAGTG CCTAGACCAT CTTATGTTTT TAGTGCTGAC 
CCAATTGCAA GGCCTTCGGA AATCAATTTT GATGGCGTTA AGCTTGATCT CTCTCATGAG 
TTTTCCTTAG TTGCCGCAAA CCCTGGGGCA AACAGTTTGG AATCTAAAAA TTACCTCCAG 
GTTTGTCTTC GAATAAGACC ATTTACACAG TCGGAAAAAG GACACGAGGC TGAGGGCTGT 
GTGCAAGTTC TGGATTCACA GACTGTTCTG CTGAAAGACC CTCAAAGCAT CCTTGGCCAC 
TTAAGTGAGA AGAGCTCCGG ACAGATGGCA CAGAAATTCA GCTTTTCTAG GGTTTTTGGA 
CCCGAAACTT CACAGAAAGA GTTCTTTCAG GGTTGCATTA TGCAGCCAGT AAAAGACCTC 
TTAAAAGGGC ACAGTCGACT GATCTTCACA TATGGGCTGA CCAATTCTGG AAAAACATAT 
ACATTTCAAG GCACAGAAGA AAACATTGGC ATTCTGCCTA GAACACTGAA TGTATTGTTT 
GATAGTCTTC AGGAAAGACT CTATACAAAG ATGAGCTTCA AACCACATAG ATGTAGAGAA 
TACCTACAGT TGTCATCTGA TCAAGAGAAA GAGGAAAGTG CTAACAAAAA TACATTGCTG 
CGACAAATTA AAGAGGTTAC CATACATAAT GACAGTTATG ACATTCTTTG TGGACGCTTA 
ACAAACTCTT TGACTATACC AGAATTTGAA GAAACCATGA ACAATTGTGA ACAATCTAGT 
CTCAATGTGG ATAATATAAA ATATTCAGTG TGGGTTTCTT TCTTTGAGAT TTACAATGAA 
AGTATTTATG ATTTGTTTGT TCCTGTGTCC TCTAAGTTGC AAAAGAGAAA AATGCTACGC 
CTTTCCCAAG ATGTAAAGGG CTATTCTTTT ATAAAAGATC TGCAGTGGAT CCAGGTATCT 
GATTCTAAAG AAGCATATAG ACTCTTAAAA CTAGGAGTGA AGCATCAGAG TGTTGCCTTC 
ACAAAACTGA ACAATGCTTC TAGCAGAAGT CACAGCATAT TCACTATTAG AATATTACAG 
ATAGAAGACT CGGAAATACC TCGTGTAACA CGAGTTAGTG AATTGTCGCT ATGTGATCTC 
GCTGGTTCAG AACGAAGCAT GAAGACGCAA AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG 
AATATCAACA CTTCTTTACT GACTCTGGGA AAGTGCATCA GTGTTCTGAA GAACAGTGAC 
AAGTCCAAGA TTCAACAACA TGTGCCTTTT CGAGAAAGTA AACTGACCCA CTATTTCCAA 
AGCTTTTTTA CTGGAAAGGG GAAAATATGT ATGATCATCA ATATTAGTCA GTGTTGTTCT 
GCCTATGATG AGACACTCAA TGTACTGAAG TTCTCAACTG TTGCACAAAA GGTCTATGTT 
CCAGATACTT TAAGTTCTTC TCAAGAGAAA TCGTTTGGGT CTACCAAATC TTTACAAGAT 
GTATCCCTAG GCAGTAATTT GGATAATAAA ATACTAAATG TAAAAAGAAA AACTGTTTCA 
TGGGAAAATA GTCTGGAAGA TGTGGTTGAA AATGAAGATT TGGTCGAGGA CTTAGAAGAA 
AATGAAGAAA CACAAAACAT GGAAACTGAA CTTACAGATG AAGATAGTGA TAAGCCGTTA 
GAGGAAGGCG GAGTCTGCGC AGGTCACGGG AAGAACAAGA AACTGTTGGA TTTAATAGAA 
AACTTGAAAA AAAGACTAAT AAATGAAAAA AAAGAAAAGT TAACATTGGA ATTTAAAATC 
CGAGAAGAAG TGACACAGGA ATTCACTCAG TATTGGAGCC AAAGGGAAGC TGATTTTAAG 
GAAACACTTC TTCATGAACG GGAGATATTA GAAGAAAATG CTGAACGTCG CTTAGCAATT 
TTCAAAGATT TGGTTGGAAA ACCTGGTGAG AGTCAAGATG AACCAGCAAG CAGATTTTGT 
ACCATGGAAC TTGAAACTGA AGAGGCTATT GCTTGTTTGC AGCTAAAGTA TAATCAAGTT 
AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA TTAATTAAAG CCCAAGAAGA GCTAAAAAAT 
AAAGAGAGTG ACTCTCTGGT TCAGGCACTC AAGACATCAA GTAAGAGCTT GTTAACTTCA 
GGTCAGATAG TTACCAAGCT GGTTTCAGTA CTTGGAGGTG AAGGCGGGAG AATCAATAAT 
ATCCAGGATA TAAAGCAGGA AAATACCGTA ACATGTAAAG TTGATACATC TTTAATCAGC 
AATAAGTCCA CTGGTAATGA AACAACTGAA ATGCCCAAGA AGAGTAGAAC CCAGACACAT 
TCAGAAAGGA AAAGATTAAA TGAAGATGGA CTTCAGCTTG GTGAACCACC AGCAAAGAAA 
GGGCTTATAC TTATTAGTCC ACCCATCACT GAAGACCAAG ACAAAAGGGA AGAAATGCAA 
CAGTCTGTTT CAGAAGGTGC AGAAGAAGAC AGTAGAGTTT TGCAGGAAAA GAATGAAGAG 
CTAAAAAGAC TTTTAACTAT TGGTGAGAAT GAACTCAGAA ATGCAAAGGA GGAAAAGGCA 
GAATTAAATA AACAGGTTGT GAGTTTGCAG CAGCAACTTT GTTTCTTTGA AGAAAAGAAC 
TCATCCCTGA GGGCAGAGGT AGAGCAAATC CAGGCCAGTT ATGACCTTGC AGCTGCTGAG 
CTGCATACTC AGAGAGCTGT GAACCAGGAG CAGAAGGACA GGATCCTGCA ATTGTCAGGG 
AAGATGGAAA CTGCGGCAAG ACGTATTGAA AGCAATGTTT CACAGATAAA GCAAATGCAA 
ACAAAAATAG ATGAACTGCG ATCTCTTGAT TCACCTTCTC ATATTTCAAA AATAGATTTA 
CTCAATCTCC AGGATCTATC AAGTGGTGCT AATTTGCTGA ACACATCACA ACAGCTTCCA 
GGTAGTGACC TACCGAGCAC ATGGGTTAAA GAGTTTCATA CTCAGGAGCT CAGCAGGGAA 
AGTTCCTTCC ACTCTAGCAT TGAAGCCATC TGGGAAGAAT GTAAAGAGAT TGTGAAGGCT 
TCCTCCAAAA AGTCTCATCA GATTCAGGGA CTAGAAGAAC TAATTGAAAA ATTACAGGTG 
GAAGTAAAAA ACTGCAGGGA TGAGAACAGT GAGCTTCGAG CAAAGGAGAG TGAGGATAAG 
AATCGAGACC AGCAGCTGAA GGAGAAAGAA AGTCTAATCC AGCAGCTTAG AGAAGAACTT 
CAGGAAACCA CTGTCAGTCT CCGTGTCCAA GTACAGCTAG TAGCGGAAAG AGAGCAAGCT 
CTTTCAGAGC TTTCCCGGGA TGTTACCTGC TATAAGGCAA AAGTAAAGGA CCTTGAAGTG 
ATGGTAGAGA CCCAGAAAGA GGAATGTAAG CGCTTAGCTG AGTTAGAGCA GAGCATTTTA 
GAGAAGGAAT CTGCCATCCT AAAGCTGGAA GCGAGTCTAA AGGAATTGGA AGCAAAGCAC 
CAGGATCACA TCAGAAGTAC CACACATTTA AATGCAGAGG AAGTCAAGTT CAGAGAAGAA 
ATTACACAGT TGGCGAATAA TTTGCATGAT ACAAAACAGT TACTTCAGTC AAAGGAAGAA 
GAAAATGAAA TTAGCAGACA AGAGACAGAA AAGTTGAAAG AGGAGCTTGC TGCAAACTCT 
GTTCTTACCC AGAATCTCCA AGCAGACCTT CAGCGGAAGG AGGAAGATTG TGCTGAGCTA 
AAGGAGAAGT TCACTGATGC CAAAAAGCAG ATCGAGCAGG TACAGAGGGA GGTATCTGTA 
ATGCGTGATG AAGAGAAGTC GTTGAGGACT AAAATTAATG AACTGGAGAA AAAGAAAAAC 
CAATATTCTC AGGAAATAGA TATGAAACAA CGAACCATTC AGCAACTTAA GGAGCAGTTA 
AGTAATCAGA AAATGGAAGA AGTTGTACAA CAGTATGAGA AAGTGTGCAA AGATCTCAGC 
GTTAAGGAGA AGCTGATTGA AGCTATGCGA CTGACACTAG TGGAGCAGGA GCAAACTCAG 
GCAGAGCAGG ACAGGATGCT TGAGGCTAAG TCACAGGAAG CTGACTGGCT AGCCGGAGAA 
TTGGATACAT GGAAAGACAA ATTCAAAGAT CTGGAGACTA GAAGTAATCA AAAGGTAACT 
ACAGAGGCCA TGGAGGACAG CGACGTGCTT AGTGAGAAGT TCAGGAAGCT TCAGGACGAG 
CTGCAGGAAT CTGAAGAGAA ACATAAAGCT GATAGAAAGA AGTGGTTAGA AGAAAAAGCG 
GTTCTTACAA CTCAAGCAAA AGAAGCAGAG ACTCTACGAA ACAGGGAGAT GAAAAAATAT 
GCTGAGGACA GGGAGCGTTG TCTGAAGCTG CAGAATGAAG TGGAAACACT GACAGCACAG 
CTGGCGGAGA AAACTGGTGA GCTTCAGAAG TGGAGAGAAG AGCGGGACCA GCTGGTCACA 
GCTGTGGAGA CACAGATGCA GGCTCTGCTC TCCAGCAGCA AGCATAAGGA CGAAGAGATC 
CAGCAACTGA GAAAGGCCGT GGCAAAGAGC ACGGGGACAG AAAACCAGAC CATGAATCTC 
AAGCCTGAAT GTAATGATTC AGTCGATCTT GGTGGAGTTG AAACTGAACT TCAGTCAACA 
AGTTTTGAAA TTTCTAGGAA TACAGCAGAG GATGGATCTG TAGTTCTTGA CTCTTGTGAA 
GTGTCAACAG AAAATGTACA AAGTACTCGA TTTCCAAAGC CTGAACTGGA GATTCAGTTT 
ACACCTCTGC AGCCAAACAA AGTGGCAGTG AAGCACCCTG GTTGTGCCAC ACCAGTCACA 
ATTAAGATCC CCAAGGCACG GAAGAGGAAG AGTGGCGAGG TGGAGGAGGA CTTGGTGAAA 
TGTGAAAATA AGAAGAATTC CACACCCAGA AGTAATGTGA AGTTTCCCGT TTCAGAGCAT 
AGAAATTCCT CTTTCAAAAA GGAACAAAAG GTTTTCTTAT GTCCGTCATC TAAGAAGACA 
TACTCCTTAC GGAGCCAAGC ATCTACGGTT GGTGCGAATG TAGCCTCTAA AAAAAAAGAG 
GGAACACTAC AGAAATTTGG AGACTTCTTA CAACATTCTC CAACAATCCT TCAATCAAAA 
GCAAAAAAGA TAATTGAAAC AATGAGTTCC CCAAAGTTAT CAACTGTAGA AGTAAGTAAA 
GAAAATGTAT CCCAGCCTAA AAAAGCCAAA CGGAAATTGT ACAGAAATGA AATTTCATCT 
CCCATCAACA TATCTGGCCA AGTGATTTTA ATGGAACAGA AAGCAAAAGA AACTGATCAT 
CAGATTCTCA AACGACGGCT TCGAACACGA ACAGCCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif20b ( XM_017589297.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444786.1 Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X2, mRNA.

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CloneID	ORa24035
Clone ID Related Accession (Same CDS sequence)	XM_017589297.1 , XM_006231281.2
Accession Version	XM_017589297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF20B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTC ATTTGAATCA AGACGGAGTG CCTAGACCAT CTTATGTTTT TAGTGCTGAC 
CCAATTGCAA GGCCTTCGGA AATCAATTTT GATGGCGTTA AGCTTGATCT CTCTCATGAG 
TTTTCCTTAG TTGCCGCAAA CCCTGGGGCA AACAGTTTGG AATCTAAAAA TTACCTCCAG 
GTTTGTCTTC GAATAAGACC ATTTACACAG TCGGAAAAAG GACACGAGGC TGAGGGCTGT 
GTGCAAGTTC TGGATTCACA GACTGTTCTG CTGAAAGACC CTCAAAGCAT CCTTGGCCAC 
TTAAGTGAGA AGAGCTCCGG ACAGATGGCA CAGAAATTCA GCTTTTCTAG GGTTTTTGGA 
CCCGAAACTT CACAGAAAGA GTTCTTTCAG GGTTGCATTA TGCAGCCAGT AAAAGACCTC 
TTAAAAGGGC ACAGTCGACT GATCTTCACA TATGGGCTGA CCAATTCTGG AAAAACATAT 
ACATTTCAAG GCACAGAAGA AAACATTGGC ATTCTGCCTA GAACACTGAA TGTATTGTTT 
GATAGTCTTC AGGAAAGACT CTATACAAAG ATGAGCTTCA AACCACATAG ATGTAGAGAA 
TACCTACAGT TGTCATCTGA TCAAGAGAAA GAGGAAAGTG CTAACAAAAA TACATTGCTG 
CGACAAATTA AAGAGGTTAC CATACATAAT GACAGTTATG ACATTCTTTG TGGACGCTTA 
ACAAACTCTT TGACTATACC AGAATTTGAA GAAACCATGA ACAATTGTGA ACAATCTAGT 
CTCAATGTGG ATAATATAAA ATATTCAGTG TGGGTTTCTT TCTTTGAGAT TTACAATGAA 
AGTATTTATG ATTTGTTTGT TCCTGTGTCC TCTAAGTTGC AAAAGAGAAA AATGCTACGC 
CTTTCCCAAG ATGTAAAGGG CTATTCTTTT ATAAAAGATC TGCAGTGGAT CCAGGTATCT 
GATTCTAAAG AAGCATATAG ACTCTTAAAA CTAGGAGTGA AGCATCAGAG TGTTGCCTTC 
ACAAAACTGA ACAATGCTTC TAGCAGAAGT CACAGCATAT TCACTATTAG AATATTACAG 
ATAGAAGACT CGGAAATACC TCGTGTAACA CGAGTTAGTG AATTGTCGCT ATGTGATCTC 
GCTGGTTCAG AACGAAGCAT GAAGACGCAA AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG 
AATATCAACA CTTCTTTACT GACTCTGGGA AAGTGCATCA GTGTTCTGAA GAACAGTGAC 
AAGTCCAAGA TTCAACAACA TGTGCCTTTT CGAGAAAGTA AACTGACCCA CTATTTCCAA 
AGCTTTTTTA CTGGAAAGGG GAAAATATGT ATGATCATCA ATATTAGTCA GTGTTGTTCT 
GCCTATGATG AGACACTCAA TGTACTGAAG TTCTCAACTG TTGCACAAAA GGTCTATGTT 
CCAGATACTT TAAGTTCTTC TCAAGAGAAA TCGTTTGGGT CTACCAAATC TTTACAAGAT 
GTATCCCTAG GCAGTAATTT GGATAATAAA ATACTAAATG TAAAAAGAAA AACTGTTTCA 
TGGGAAAATA GTCTGGAAGA TGTGGTTGAA AATGAAGATT TGGTCGAGGA CTTAGAAGAA 
AATGAAGAAA CACAAAACAT GGAAACTGAA CTTACAGATG AAGATAGTGA TAAGCCGTTA 
GAGGAAGGCG GAGTCTGCGC AGGTCACGGG AAGAACAAGA AACTGTTGGA TTTAATAGAA 
AACTTGAAAA AAAGACTAAT AAATGAAAAA AAAGAAAAGT TAACATTGGA ATTTAAAATC 
CGAGAAGAAG TGACACAGGA ATTCACTCAG TATTGGAGCC AAAGGGAAGC TGATTTTAAG 
GAAACACTTC TTCATGAACG GGAGATATTA GAAGAAAATG CTGAACGTCG CTTAGCAATT 
TTCAAAGATT TGGTTGGAAA ACCTGGTGAG AGTCAAGATG AACCAGCAAG CAGATTTTGT 
ACCATGGAAC TTGAAACTGA AGAATCTCAT AATTATGTAG GAGTTGAAGA TATTTTTGAT 
TCTCTTGAAG ATGATGTCAC TGATATTAAG AAACAGGCCG AACTTGCTCA TTTGTATATT 
ACATCTCTTG TTGATCCCCA GGAGGCTATT GCTTGTTTGC AGCTAAAGTA TAATCAAGTT 
AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA TTAATTAAAG CCCAAGAAGA GCTAAAAAAT 
AAAGAGAGTG ACTCTCTGGT TCAGGCACTC AAGACATCAA GTAAGGTTGA TACATCTTTA 
ATCAGCAATA AGTCCACTGG TAATGAAACA ACTGAAATGC CCAAGAAGAG TAGAACCCAG 
ACACATTCAG AAAGGAAAAG ATTAAATGAA GATGGACTTC AGCTTGGTGA ACCACCAGCA 
AAGAAAGGGC TTATACTTAT TAGTCCACCC ATCACTGAAG ACCAAGACAA AAGGGAAGAA 
ATGCAACAGT CTGTTTCAGA AGGTGCAGAA GAAGACAGTA GAGTTTTGCA GGAAAAGAAT 
GAAGAGCTAA AAAGACTTTT AACTATTGGT GAGAATGAAC TCAGAAATGC AAAGGAGGAA 
AAGGCAGAAT TAAATAAACA GGTTGTGAGT TTGCAGCAGC AACTTTGTTT CTTTGAAGAA 
AAGAACTCAT CCCTGAGGGC AGAGGTAGAG CAAATCCAGG CCAGTTATGA CCTTGCAGCT 
GCTGAGCTGC ATACTCAGAG AGCTGTGAAC CAGGAGCAGA AGGACAGGAT CCTGCAATTG 
TCAGGGAAGA TGGAAACTGC GGCAAGACGT ATTGAAAGCA ATGTTTCACA GATAAAGCAA 
ATGCAAACAA AAATAGATGA ACTGCGATCT CTTGATTCAC CTTCTCATAT TTCAAAAATA 
GATTTACTCA ATCTCCAGGA TCTATCAAGT GGTGCTAATT TGCTGAACAC ATCACAACAG 
CTTCCAGGTA GTGACCTACC GAGCACATGG GTTAAAGAGT TTCATACTCA GGAGCTCAGC 
AGGGAAAGTT CCTTCCACTC TAGCATTGAA GCCATCTGGG AAGAATGTAA AGAGATTGTG 
AAGGCTTCCT CCAAAAAGTC TCATCAGATT CAGGGACTAG AAGAACTAAT TGAAAAATTA 
CAGGTGGAAG TAAAAAACTG CAGGGATGAG AACAGTGAGC TTCGAGCAAA GGAGAGTGAG 
GATAAGAATC GAGACCAGCA GCTGAAGGAG AAAGAAAGTC TAATCCAGCA GCTTAGAGAA 
GAACTTCAGG AAACCACTGT CAGTCTCCGT GTCCAAGTAC AGCTAGTAGC GGAAAGAGAG 
CAAGCTCTTT CAGAGCTTTC CCGGGATGTT ACCTGCTATA AGGCAAAAGT AAAGGACCTT 
GAAGTGATGG TAGAGACCCA GAAAGAGGAA TGTAAGCGCT TAGCTGAGTT AGAGCAGAGC 
ATTTTAGAGA AGGAATCTGC CATCCTAAAG CTGGAAGCGA GTCTAAAGGA ATTGGAAGCA 
AAGCACCAGG ATCACATCAG AAGTACCACA CATTTAAATG CAGAGGAAGT CAAGTTCAGA 
GAAGAAATTA CACAGTTGGC GAATAATTTG CATGATACAA AACAGTTACT TCAGTCAAAG 
GAAGAAGAAA ATGAAATTAG CAGACAAGAG ACAGAAAAGT TGAAAGAGGA GCTTGCTGCA 
AACTCTGTTC TTACCCAGAA TCTCCAAGCA GACCTTCAGC GGAAGGAGGA AGATTGTGCT 
GAGCTAAAGG AGAAGTTCAC TGATGCCAAA AAGCAGATCG AGCAGGTACA GAGGGAGGTA 
TCTGTAATGC GTGATGAAGA GAAGTCGTTG AGGACTAAAA TTAATGAACT GGAGAAAAAG 
AAAAACCAAT ATTCTCAGGA AATAGATATG AAACAACGAA CCATTCAGCA ACTTAAGGAG 
CAGTTAAGTA ATCAGAAAAT GGAAGAAGTT GTACAACAGT ATGAGAAAGT GTGCAAAGAT 
CTCAGCGTTA AGGAGAAGCT GATTGAAGCT ATGCGACTGA CACTAGTGGA GCAGGAGCAA 
ACTCAGGCAG AGCAGGACAG GATGCTTGAG GCTAAGTCAC AGGAAGCTGA CTGGCTAGCC 
GGAGAATTGG ATACATGGAA AGACAAATTC AAAGATCTGG AGACTAGAAG TAATCAAAAG 
GTAACTACAG AGGCCATGGA GGACAGCGAC GTGCTTAGTG AGAAGTTCAG GAAGCTTCAG 
GACGAGCTGC AGGAATCTGA AGAGAAACAT AAAGCTGATA GAAAGAAGTG GTTAGAAGAA 
AAAGCGGTTC TTACAACTCA AGCAAAAGAA GCAGAGACTC TACGAAACAG GGAGATGAAA 
AAATATGCTG AGGACAGGGA GCGTTGTCTG AAGCTGCAGA ATGAAGTGGA AACACTGACA 
GCACAGCTGG CGGAGAAAAC TGGTGAGCTT CAGAAGTGGA GAGAAGAGCG GGACCAGCTG 
GTCACAGCTG TGGAGACACA GATGCAGGCT CTGCTCTCCA GCAGCAAGCA TAAGGACGAA 
GAGATCCAGC AACTGAGAAA GGCCGTGGCA AAGAGCACGG GGACAGTGAG TGGACGCGTA 
CTCGAAAACC AGACCATGAA TCTCAAGCCT GAATGTAATG ATTCAGTCGA TCTTGGTGGA 
GTTGAAACTG AACTTCAGTC AACAAGTTTT GAAATTTCTA GGAATACAGC AGAGGATGGA 
TCTGTAGTTC TTGACTCTTG TGAAGTGTCA ACAGAAAATG TACAAAGTAC TCGATTTCCA 
AAGCCTGAAC TGGAGATTCA GTTTACACCT CTGCAGCCAA ACAAAGTGGC AGTGAAGCAC 
CCTGGTTGTG CCACACCAGT CACAATTAAG ATCCCCAAGG CACGGAAGAG GAAGAGTGGC 
GAGGTGGAGG AGGACTTGGT GAAATGTGAA AATAAGAAGA ATTCCACACC CAGAAGTAAT 
GTGAAGTTTC CCGTTTCAGA GCATAGAAAT TCCTCTTTCA AAAAGGAACA AAAGGTTTTC 
TTATGTCCGT CATCTAAGAA GACATACTCC TTACGGAGCC AAGCATCTAC GGTTGGTGCG 
AATGTAGCCT CTAAAAAAAA AGAGGGAACA CTACAGAAAT TTGGAGACTT CTTACAACAT 
TCTCCAACAA TCCTTCAATC AAAAGCAAAA AAGATAATTG AAACAATGAG TTCCCCAAAG 
TTATCAACTG TAGAAGTAAG TAAAGAAAAT GTATCCCAGC CTAAAAAAGC CAAACGGAAA 
TTGTACAGAA ATGAAATTTC ATCTCCCATC AACATATCTG GCCAAGTGAT TTTAATGGAA 
CAGAAAGCAA AAGAAACTGA TCATCAGATT CTCAAACGAC GGCTTCGAAC ACGAACAGCC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444786.1
CDS	136..5481
Translation	MESHLNQDGVPRPSYVFSADPIARPSEINFDGVKLDLSHEFSLVAANPGANSLESKNYLQVCLRIRPFTQSEKGHEAEGCVQVLDSQTVLLKDPQSILGHLSEKSSGQMAQKFSFSRVFGPETSQKEFFQGCIMQPVKDLLKGHSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLFDSLQERLYTKMSFKPHRCREYLQLSSDQEKEESANKNTLLRQIKEVTIHNDSYDILCGRLTNSLTIPEFEETMNNCEQSSLNVDNIKYSVWVSFFEIYNESIYDLFVPVSSKLQKRKMLRLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGVKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPRVTRVSELSLCDLAGSERSMKTQSEGERLREAGNINTSLLTLGKCISVLKNSDKSKIQQHVPFRESKLTHYFQSFFTGKGKICMIINISQCCSAYDETLNVLKFSTVAQKVYVPDTLSSSQEKSFGSTKSLQDVSLGSNLDNKILNVKRKTVSWENSLEDVVENEDLVEDLEENEETQNMETELTDEDSDKPLEEGGVCAGHGKNKKLLDLIENLKKRLINEKKEKLTLEFKIREEVTQEFTQYWSQREADFKETLLHEREILEENAERRLAIFKDLVGKPGESQDEPASRFCTMELETEESHNYVGVEDIFDSLEDDVTDIKKQAELAHLYITSLVDPQEAIACLQLKYNQVKAELAETKEELIKAQEELKNKESDSLVQALKTSSKVDTSLISNKSTGNETTEMPKKSRTQTHSERKRLNEDGLQLGEPPAKKGLILISPPITEDQDKREEMQQSVSEGAEEDSRVLQEKNEELKRLLTIGENELRNAKEEKAELNKQVVSLQQQLCFFEEKNSSLRAEVEQIQASYDLAAAELHTQRAVNQEQKDRILQLSGKMETAARRIESNVSQIKQMQTKIDELRSLDSPSHISKIDLLNLQDLSSGANLLNTSQQLPGSDLPSTWVKEFHTQELSRESSFHSSIEAIWEECKEIVKASSKKSHQIQGLEELIEKLQVEVKNCRDENSELRAKESEDKNRDQQLKEKESLIQQLREELQETTVSLRVQVQLVAEREQALSELSRDVTCYKAKVKDLEVMVETQKEECKRLAELEQSILEKESAILKLEASLKELEAKHQDHIRSTTHLNAEEVKFREEITQLANNLHDTKQLLQSKEEENEISRQETEKLKEELAANSVLTQNLQADLQRKEEDCAELKEKFTDAKKQIEQVQREVSVMRDEEKSLRTKINELEKKKNQYSQEIDMKQRTIQQLKEQLSNQKMEEVVQQYEKVCKDLSVKEKLIEAMRLTLVEQEQTQAEQDRMLEAKSQEADWLAGELDTWKDKFKDLETRSNQKVTTEAMEDSDVLSEKFRKLQDELQESEEKHKADRKKWLEEKAVLTTQAKEAETLRNREMKKYAEDRERCLKLQNEVETLTAQLAEKTGELQKWREERDQLVTAVETQMQALLSSSKHKDEEIQQLRKAVAKSTGTVSGRVLENQTMNLKPECNDSVDLGGVETELQSTSFEISRNTAEDGSVVLDSCEVSTENVQSTRFPKPELEIQFTPLQPNKVAVKHPGCATPVTIKIPKARKRKSGEVEEDLVKCENKKNSTPRSNVKFPVSEHRNSSFKKEQKVFLCPSSKKTYSLRSQASTVGANVASKKKEGTLQKFGDFLQHSPTILQSKAKKIIETMSSPKLSTVEVSKENVSQPKKAKRKLYRNEISSPINISGQVILMEQKAKETDHQILKRRLRTRTAK

Target ORF information:

RefSeq Version	XM_017589297.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589297.1

ORF Insert Sequence:

ATGGAATCTC ATTTGAATCA AGACGGAGTG CCTAGACCAT CTTATGTTTT TAGTGCTGAC 
CCAATTGCAA GGCCTTCGGA AATCAATTTT GATGGCGTTA AGCTTGATCT CTCTCATGAG 
TTTTCCTTAG TTGCCGCAAA CCCTGGGGCA AACAGTTTGG AATCTAAAAA TTACCTCCAG 
GTTTGTCTTC GAATAAGACC ATTTACACAG TCGGAAAAAG GACACGAGGC TGAGGGCTGT 
GTGCAAGTTC TGGATTCACA GACTGTTCTG CTGAAAGACC CTCAAAGCAT CCTTGGCCAC 
TTAAGTGAGA AGAGCTCCGG ACAGATGGCA CAGAAATTCA GCTTTTCTAG GGTTTTTGGA 
CCCGAAACTT CACAGAAAGA GTTCTTTCAG GGTTGCATTA TGCAGCCAGT AAAAGACCTC 
TTAAAAGGGC ACAGTCGACT GATCTTCACA TATGGGCTGA CCAATTCTGG AAAAACATAT 
ACATTTCAAG GCACAGAAGA AAACATTGGC ATTCTGCCTA GAACACTGAA TGTATTGTTT 
GATAGTCTTC AGGAAAGACT CTATACAAAG ATGAGCTTCA AACCACATAG ATGTAGAGAA 
TACCTACAGT TGTCATCTGA TCAAGAGAAA GAGGAAAGTG CTAACAAAAA TACATTGCTG 
CGACAAATTA AAGAGGTTAC CATACATAAT GACAGTTATG ACATTCTTTG TGGACGCTTA 
ACAAACTCTT TGACTATACC AGAATTTGAA GAAACCATGA ACAATTGTGA ACAATCTAGT 
CTCAATGTGG ATAATATAAA ATATTCAGTG TGGGTTTCTT TCTTTGAGAT TTACAATGAA 
AGTATTTATG ATTTGTTTGT TCCTGTGTCC TCTAAGTTGC AAAAGAGAAA AATGCTACGC 
CTTTCCCAAG ATGTAAAGGG CTATTCTTTT ATAAAAGATC TGCAGTGGAT CCAGGTATCT 
GATTCTAAAG AAGCATATAG ACTCTTAAAA CTAGGAGTGA AGCATCAGAG TGTTGCCTTC 
ACAAAACTGA ACAATGCTTC TAGCAGAAGT CACAGCATAT TCACTATTAG AATATTACAG 
ATAGAAGACT CGGAAATACC TCGTGTAACA CGAGTTAGTG AATTGTCGCT ATGTGATCTC 
GCTGGTTCAG AACGAAGCAT GAAGACGCAA AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG 
AATATCAACA CTTCTTTACT GACTCTGGGA AAGTGCATCA GTGTTCTGAA GAACAGTGAC 
AAGTCCAAGA TTCAACAACA TGTGCCTTTT CGAGAAAGTA AACTGACCCA CTATTTCCAA 
AGCTTTTTTA CTGGAAAGGG GAAAATATGT ATGATCATCA ATATTAGTCA GTGTTGTTCT 
GCCTATGATG AGACACTCAA TGTACTGAAG TTCTCAACTG TTGCACAAAA GGTCTATGTT 
CCAGATACTT TAAGTTCTTC TCAAGAGAAA TCGTTTGGGT CTACCAAATC TTTACAAGAT 
GTATCCCTAG GCAGTAATTT GGATAATAAA ATACTAAATG TAAAAAGAAA AACTGTTTCA 
TGGGAAAATA GTCTGGAAGA TGTGGTTGAA AATGAAGATT TGGTCGAGGA CTTAGAAGAA 
AATGAAGAAA CACAAAACAT GGAAACTGAA CTTACAGATG AAGATAGTGA TAAGCCGTTA 
GAGGAAGGCG GAGTCTGCGC AGGTCACGGG AAGAACAAGA AACTGTTGGA TTTAATAGAA 
AACTTGAAAA AAAGACTAAT AAATGAAAAA AAAGAAAAGT TAACATTGGA ATTTAAAATC 
CGAGAAGAAG TGACACAGGA ATTCACTCAG TATTGGAGCC AAAGGGAAGC TGATTTTAAG 
GAAACACTTC TTCATGAACG GGAGATATTA GAAGAAAATG CTGAACGTCG CTTAGCAATT 
TTCAAAGATT TGGTTGGAAA ACCTGGTGAG AGTCAAGATG AACCAGCAAG CAGATTTTGT 
ACCATGGAAC TTGAAACTGA AGAATCTCAT AATTATGTAG GAGTTGAAGA TATTTTTGAT 
TCTCTTGAAG ATGATGTCAC TGATATTAAG AAACAGGCCG AACTTGCTCA TTTGTATATT 
ACATCTCTTG TTGATCCCCA GGAGGCTATT GCTTGTTTGC AGCTAAAGTA TAATCAAGTT 
AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA TTAATTAAAG CCCAAGAAGA GCTAAAAAAT 
AAAGAGAGTG ACTCTCTGGT TCAGGCACTC AAGACATCAA GTAAGGTTGA TACATCTTTA 
ATCAGCAATA AGTCCACTGG TAATGAAACA ACTGAAATGC CCAAGAAGAG TAGAACCCAG 
ACACATTCAG AAAGGAAAAG ATTAAATGAA GATGGACTTC AGCTTGGTGA ACCACCAGCA 
AAGAAAGGGC TTATACTTAT TAGTCCACCC ATCACTGAAG ACCAAGACAA AAGGGAAGAA 
ATGCAACAGT CTGTTTCAGA AGGTGCAGAA GAAGACAGTA GAGTTTTGCA GGAAAAGAAT 
GAAGAGCTAA AAAGACTTTT AACTATTGGT GAGAATGAAC TCAGAAATGC AAAGGAGGAA 
AAGGCAGAAT TAAATAAACA GGTTGTGAGT TTGCAGCAGC AACTTTGTTT CTTTGAAGAA 
AAGAACTCAT CCCTGAGGGC AGAGGTAGAG CAAATCCAGG CCAGTTATGA CCTTGCAGCT 
GCTGAGCTGC ATACTCAGAG AGCTGTGAAC CAGGAGCAGA AGGACAGGAT CCTGCAATTG 
TCAGGGAAGA TGGAAACTGC GGCAAGACGT ATTGAAAGCA ATGTTTCACA GATAAAGCAA 
ATGCAAACAA AAATAGATGA ACTGCGATCT CTTGATTCAC CTTCTCATAT TTCAAAAATA 
GATTTACTCA ATCTCCAGGA TCTATCAAGT GGTGCTAATT TGCTGAACAC ATCACAACAG 
CTTCCAGGTA GTGACCTACC GAGCACATGG GTTAAAGAGT TTCATACTCA GGAGCTCAGC 
AGGGAAAGTT CCTTCCACTC TAGCATTGAA GCCATCTGGG AAGAATGTAA AGAGATTGTG 
AAGGCTTCCT CCAAAAAGTC TCATCAGATT CAGGGACTAG AAGAACTAAT TGAAAAATTA 
CAGGTGGAAG TAAAAAACTG CAGGGATGAG AACAGTGAGC TTCGAGCAAA GGAGAGTGAG 
GATAAGAATC GAGACCAGCA GCTGAAGGAG AAAGAAAGTC TAATCCAGCA GCTTAGAGAA 
GAACTTCAGG AAACCACTGT CAGTCTCCGT GTCCAAGTAC AGCTAGTAGC GGAAAGAGAG 
CAAGCTCTTT CAGAGCTTTC CCGGGATGTT ACCTGCTATA AGGCAAAAGT AAAGGACCTT 
GAAGTGATGG TAGAGACCCA GAAAGAGGAA TGTAAGCGCT TAGCTGAGTT AGAGCAGAGC 
ATTTTAGAGA AGGAATCTGC CATCCTAAAG CTGGAAGCGA GTCTAAAGGA ATTGGAAGCA 
AAGCACCAGG ATCACATCAG AAGTACCACA CATTTAAATG CAGAGGAAGT CAAGTTCAGA 
GAAGAAATTA CACAGTTGGC GAATAATTTG CATGATACAA AACAGTTACT TCAGTCAAAG 
GAAGAAGAAA ATGAAATTAG CAGACAAGAG ACAGAAAAGT TGAAAGAGGA GCTTGCTGCA 
AACTCTGTTC TTACCCAGAA TCTCCAAGCA GACCTTCAGC GGAAGGAGGA AGATTGTGCT 
GAGCTAAAGG AGAAGTTCAC TGATGCCAAA AAGCAGATCG AGCAGGTACA GAGGGAGGTA 
TCTGTAATGC GTGATGAAGA GAAGTCGTTG AGGACTAAAA TTAATGAACT GGAGAAAAAG 
AAAAACCAAT ATTCTCAGGA AATAGATATG AAACAACGAA CCATTCAGCA ACTTAAGGAG 
CAGTTAAGTA ATCAGAAAAT GGAAGAAGTT GTACAACAGT ATGAGAAAGT GTGCAAAGAT 
CTCAGCGTTA AGGAGAAGCT GATTGAAGCT ATGCGACTGA CACTAGTGGA GCAGGAGCAA 
ACTCAGGCAG AGCAGGACAG GATGCTTGAG GCTAAGTCAC AGGAAGCTGA CTGGCTAGCC 
GGAGAATTGG ATACATGGAA AGACAAATTC AAAGATCTGG AGACTAGAAG TAATCAAAAG 
GTAACTACAG AGGCCATGGA GGACAGCGAC GTGCTTAGTG AGAAGTTCAG GAAGCTTCAG 
GACGAGCTGC AGGAATCTGA AGAGAAACAT AAAGCTGATA GAAAGAAGTG GTTAGAAGAA 
AAAGCGGTTC TTACAACTCA AGCAAAAGAA GCAGAGACTC TACGAAACAG GGAGATGAAA 
AAATATGCTG AGGACAGGGA GCGTTGTCTG AAGCTGCAGA ATGAAGTGGA AACACTGACA 
GCACAGCTGG CGGAGAAAAC TGGTGAGCTT CAGAAGTGGA GAGAAGAGCG GGACCAGCTG 
GTCACAGCTG TGGAGACACA GATGCAGGCT CTGCTCTCCA GCAGCAAGCA TAAGGACGAA 
GAGATCCAGC AACTGAGAAA GGCCGTGGCA AAGAGCACGG GGACAGTGAG TGGACGCGTA 
CTCGAAAACC AGACCATGAA TCTCAAGCCT GAATGTAATG ATTCAGTCGA TCTTGGTGGA 
GTTGAAACTG AACTTCAGTC AACAAGTTTT GAAATTTCTA GGAATACAGC AGAGGATGGA 
TCTGTAGTTC TTGACTCTTG TGAAGTGTCA ACAGAAAATG TACAAAGTAC TCGATTTCCA 
AAGCCTGAAC TGGAGATTCA GTTTACACCT CTGCAGCCAA ACAAAGTGGC AGTGAAGCAC 
CCTGGTTGTG CCACACCAGT CACAATTAAG ATCCCCAAGG CACGGAAGAG GAAGAGTGGC 
GAGGTGGAGG AGGACTTGGT GAAATGTGAA AATAAGAAGA ATTCCACACC CAGAAGTAAT 
GTGAAGTTTC CCGTTTCAGA GCATAGAAAT TCCTCTTTCA AAAAGGAACA AAAGGTTTTC 
TTATGTCCGT CATCTAAGAA GACATACTCC TTACGGAGCC AAGCATCTAC GGTTGGTGCG 
AATGTAGCCT CTAAAAAAAA AGAGGGAACA CTACAGAAAT TTGGAGACTT CTTACAACAT 
TCTCCAACAA TCCTTCAATC AAAAGCAAAA AAGATAATTG AAACAATGAG TTCCCCAAAG 
TTATCAACTG TAGAAGTAAG TAAAGAAAAT GTATCCCAGC CTAAAAAAGC CAAACGGAAA 
TTGTACAGAA ATGAAATTTC ATCTCCCATC AACATATCTG GCCAAGTGAT TTTAATGGAA 
CAGAAAGCAA AAGAAACTGA TCATCAGATT CTCAAACGAC GGCTTCGAAC ACGAACAGCC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif20b ( XM_006231281.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231343.1 Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X1, mRNA.

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CloneID	ORa24035
Clone ID Related Accession (Same CDS sequence)	XM_017589297.1 , XM_006231281.2
Accession Version	XM_006231281.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF20B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006231281.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTC ATTTGAATCA AGACGGAGTG CCTAGACCAT CTTATGTTTT TAGTGCTGAC 
CCAATTGCAA GGCCTTCGGA AATCAATTTT GATGGCGTTA AGCTTGATCT CTCTCATGAG 
TTTTCCTTAG TTGCCGCAAA CCCTGGGGCA AACAGTTTGG AATCTAAAAA TTACCTCCAG 
GTTTGTCTTC GAATAAGACC ATTTACACAG TCGGAAAAAG GACACGAGGC TGAGGGCTGT 
GTGCAAGTTC TGGATTCACA GACTGTTCTG CTGAAAGACC CTCAAAGCAT CCTTGGCCAC 
TTAAGTGAGA AGAGCTCCGG ACAGATGGCA CAGAAATTCA GCTTTTCTAG GGTTTTTGGA 
CCCGAAACTT CACAGAAAGA GTTCTTTCAG GGTTGCATTA TGCAGCCAGT AAAAGACCTC 
TTAAAAGGGC ACAGTCGACT GATCTTCACA TATGGGCTGA CCAATTCTGG AAAAACATAT 
ACATTTCAAG GCACAGAAGA AAACATTGGC ATTCTGCCTA GAACACTGAA TGTATTGTTT 
GATAGTCTTC AGGAAAGACT CTATACAAAG ATGAGCTTCA AACCACATAG ATGTAGAGAA 
TACCTACAGT TGTCATCTGA TCAAGAGAAA GAGGAAAGTG CTAACAAAAA TACATTGCTG 
CGACAAATTA AAGAGGTTAC CATACATAAT GACAGTTATG ACATTCTTTG TGGACGCTTA 
ACAAACTCTT TGACTATACC AGAATTTGAA GAAACCATGA ACAATTGTGA ACAATCTAGT 
CTCAATGTGG ATAATATAAA ATATTCAGTG TGGGTTTCTT TCTTTGAGAT TTACAATGAA 
AGTATTTATG ATTTGTTTGT TCCTGTGTCC TCTAAGTTGC AAAAGAGAAA AATGCTACGC 
CTTTCCCAAG ATGTAAAGGG CTATTCTTTT ATAAAAGATC TGCAGTGGAT CCAGGTATCT 
GATTCTAAAG AAGCATATAG ACTCTTAAAA CTAGGAGTGA AGCATCAGAG TGTTGCCTTC 
ACAAAACTGA ACAATGCTTC TAGCAGAAGT CACAGCATAT TCACTATTAG AATATTACAG 
ATAGAAGACT CGGAAATACC TCGTGTAACA CGAGTTAGTG AATTGTCGCT ATGTGATCTC 
GCTGGTTCAG AACGAAGCAT GAAGACGCAA AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG 
AATATCAACA CTTCTTTACT GACTCTGGGA AAGTGCATCA GTGTTCTGAA GAACAGTGAC 
AAGTCCAAGA TTCAACAACA TGTGCCTTTT CGAGAAAGTA AACTGACCCA CTATTTCCAA 
AGCTTTTTTA CTGGAAAGGG GAAAATATGT ATGATCATCA ATATTAGTCA GTGTTGTTCT 
GCCTATGATG AGACACTCAA TGTACTGAAG TTCTCAACTG TTGCACAAAA GGTCTATGTT 
CCAGATACTT TAAGTTCTTC TCAAGAGAAA TCGTTTGGGT CTACCAAATC TTTACAAGAT 
GTATCCCTAG GCAGTAATTT GGATAATAAA ATACTAAATG TAAAAAGAAA AACTGTTTCA 
TGGGAAAATA GTCTGGAAGA TGTGGTTGAA AATGAAGATT TGGTCGAGGA CTTAGAAGAA 
AATGAAGAAA CACAAAACAT GGAAACTGAA CTTACAGATG AAGATAGTGA TAAGCCGTTA 
GAGGAAGGCG GAGTCTGCGC AGGTCACGGG AAGAACAAGA AACTGTTGGA TTTAATAGAA 
AACTTGAAAA AAAGACTAAT AAATGAAAAA AAAGAAAAGT TAACATTGGA ATTTAAAATC 
CGAGAAGAAG TGACACAGGA ATTCACTCAG TATTGGAGCC AAAGGGAAGC TGATTTTAAG 
GAAACACTTC TTCATGAACG GGAGATATTA GAAGAAAATG CTGAACGTCG CTTAGCAATT 
TTCAAAGATT TGGTTGGAAA ACCTGGTGAG AGTCAAGATG AACCAGCAAG CAGATTTTGT 
ACCATGGAAC TTGAAACTGA AGAATCTCAT AATTATGTAG GAGTTGAAGA TATTTTTGAT 
TCTCTTGAAG ATGATGTCAC TGATATTAAG AAACAGGCCG AACTTGCTCA TTTGTATATT 
ACATCTCTTG TTGATCCCCA GGAGGCTATT GCTTGTTTGC AGCTAAAGTA TAATCAAGTT 
AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA TTAATTAAAG CCCAAGAAGA GCTAAAAAAT 
AAAGAGAGTG ACTCTCTGGT TCAGGCACTC AAGACATCAA GTAAGGTTGA TACATCTTTA 
ATCAGCAATA AGTCCACTGG TAATGAAACA ACTGAAATGC CCAAGAAGAG TAGAACCCAG 
ACACATTCAG AAAGGAAAAG ATTAAATGAA GATGGACTTC AGCTTGGTGA ACCACCAGCA 
AAGAAAGGGC TTATACTTAT TAGTCCACCC ATCACTGAAG ACCAAGACAA AAGGGAAGAA 
ATGCAACAGT CTGTTTCAGA AGGTGCAGAA GAAGACAGTA GAGTTTTGCA GGAAAAGAAT 
GAAGAGCTAA AAAGACTTTT AACTATTGGT GAGAATGAAC TCAGAAATGC AAAGGAGGAA 
AAGGCAGAAT TAAATAAACA GGTTGTGAGT TTGCAGCAGC AACTTTGTTT CTTTGAAGAA 
AAGAACTCAT CCCTGAGGGC AGAGGTAGAG CAAATCCAGG CCAGTTATGA CCTTGCAGCT 
GCTGAGCTGC ATACTCAGAG AGCTGTGAAC CAGGAGCAGA AGGACAGGAT CCTGCAATTG 
TCAGGGAAGA TGGAAACTGC GGCAAGACGT ATTGAAAGCA ATGTTTCACA GATAAAGCAA 
ATGCAAACAA AAATAGATGA ACTGCGATCT CTTGATTCAC CTTCTCATAT TTCAAAAATA 
GATTTACTCA ATCTCCAGGA TCTATCAAGT GGTGCTAATT TGCTGAACAC ATCACAACAG 
CTTCCAGGTA GTGACCTACC GAGCACATGG GTTAAAGAGT TTCATACTCA GGAGCTCAGC 
AGGGAAAGTT CCTTCCACTC TAGCATTGAA GCCATCTGGG AAGAATGTAA AGAGATTGTG 
AAGGCTTCCT CCAAAAAGTC TCATCAGATT CAGGGACTAG AAGAACTAAT TGAAAAATTA 
CAGGTGGAAG TAAAAAACTG CAGGGATGAG AACAGTGAGC TTCGAGCAAA GGAGAGTGAG 
GATAAGAATC GAGACCAGCA GCTGAAGGAG AAAGAAAGTC TAATCCAGCA GCTTAGAGAA 
GAACTTCAGG AAACCACTGT CAGTCTCCGT GTCCAAGTAC AGCTAGTAGC GGAAAGAGAG 
CAAGCTCTTT CAGAGCTTTC CCGGGATGTT ACCTGCTATA AGGCAAAAGT AAAGGACCTT 
GAAGTGATGG TAGAGACCCA GAAAGAGGAA TGTAAGCGCT TAGCTGAGTT AGAGCAGAGC 
ATTTTAGAGA AGGAATCTGC CATCCTAAAG CTGGAAGCGA GTCTAAAGGA ATTGGAAGCA 
AAGCACCAGG ATCACATCAG AAGTACCACA CATTTAAATG CAGAGGAAGT CAAGTTCAGA 
GAAGAAATTA CACAGTTGGC GAATAATTTG CATGATACAA AACAGTTACT TCAGTCAAAG 
GAAGAAGAAA ATGAAATTAG CAGACAAGAG ACAGAAAAGT TGAAAGAGGA GCTTGCTGCA 
AACTCTGTTC TTACCCAGAA TCTCCAAGCA GACCTTCAGC GGAAGGAGGA AGATTGTGCT 
GAGCTAAAGG AGAAGTTCAC TGATGCCAAA AAGCAGATCG AGCAGGTACA GAGGGAGGTA 
TCTGTAATGC GTGATGAAGA GAAGTCGTTG AGGACTAAAA TTAATGAACT GGAGAAAAAG 
AAAAACCAAT ATTCTCAGGA AATAGATATG AAACAACGAA CCATTCAGCA ACTTAAGGAG 
CAGTTAAGTA ATCAGAAAAT GGAAGAAGTT GTACAACAGT ATGAGAAAGT GTGCAAAGAT 
CTCAGCGTTA AGGAGAAGCT GATTGAAGCT ATGCGACTGA CACTAGTGGA GCAGGAGCAA 
ACTCAGGCAG AGCAGGACAG GATGCTTGAG GCTAAGTCAC AGGAAGCTGA CTGGCTAGCC 
GGAGAATTGG ATACATGGAA AGACAAATTC AAAGATCTGG AGACTAGAAG TAATCAAAAG 
GTAACTACAG AGGCCATGGA GGACAGCGAC GTGCTTAGTG AGAAGTTCAG GAAGCTTCAG 
GACGAGCTGC AGGAATCTGA AGAGAAACAT AAAGCTGATA GAAAGAAGTG GTTAGAAGAA 
AAAGCGGTTC TTACAACTCA AGCAAAAGAA GCAGAGACTC TACGAAACAG GGAGATGAAA 
AAATATGCTG AGGACAGGGA GCGTTGTCTG AAGCTGCAGA ATGAAGTGGA AACACTGACA 
GCACAGCTGG CGGAGAAAAC TGGTGAGCTT CAGAAGTGGA GAGAAGAGCG GGACCAGCTG 
GTCACAGCTG TGGAGACACA GATGCAGGCT CTGCTCTCCA GCAGCAAGCA TAAGGACGAA 
GAGATCCAGC AACTGAGAAA GGCCGTGGCA AAGAGCACGG GGACAGTGAG TGGACGCGTA 
CTCGAAAACC AGACCATGAA TCTCAAGCCT GAATGTAATG ATTCAGTCGA TCTTGGTGGA 
GTTGAAACTG AACTTCAGTC AACAAGTTTT GAAATTTCTA GGAATACAGC AGAGGATGGA 
TCTGTAGTTC TTGACTCTTG TGAAGTGTCA ACAGAAAATG TACAAAGTAC TCGATTTCCA 
AAGCCTGAAC TGGAGATTCA GTTTACACCT CTGCAGCCAA ACAAAGTGGC AGTGAAGCAC 
CCTGGTTGTG CCACACCAGT CACAATTAAG ATCCCCAAGG CACGGAAGAG GAAGAGTGGC 
GAGGTGGAGG AGGACTTGGT GAAATGTGAA AATAAGAAGA ATTCCACACC CAGAAGTAAT 
GTGAAGTTTC CCGTTTCAGA GCATAGAAAT TCCTCTTTCA AAAAGGAACA AAAGGTTTTC 
TTATGTCCGT CATCTAAGAA GACATACTCC TTACGGAGCC AAGCATCTAC GGTTGGTGCG 
AATGTAGCCT CTAAAAAAAA AGAGGGAACA CTACAGAAAT TTGGAGACTT CTTACAACAT 
TCTCCAACAA TCCTTCAATC AAAAGCAAAA AAGATAATTG AAACAATGAG TTCCCCAAAG 
TTATCAACTG TAGAAGTAAG TAAAGAAAAT GTATCCCAGC CTAAAAAAGC CAAACGGAAA 
TTGTACAGAA ATGAAATTTC ATCTCCCATC AACATATCTG GCCAAGTGAT TTTAATGGAA 
CAGAAAGCAA AAGAAACTGA TCATCAGATT CTCAAACGAC GGCTTCGAAC ACGAACAGCC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231343.1
CDS	120..5465
Translation	MESHLNQDGVPRPSYVFSADPIARPSEINFDGVKLDLSHEFSLVAANPGANSLESKNYLQVCLRIRPFTQSEKGHEAEGCVQVLDSQTVLLKDPQSILGHLSEKSSGQMAQKFSFSRVFGPETSQKEFFQGCIMQPVKDLLKGHSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLFDSLQERLYTKMSFKPHRCREYLQLSSDQEKEESANKNTLLRQIKEVTIHNDSYDILCGRLTNSLTIPEFEETMNNCEQSSLNVDNIKYSVWVSFFEIYNESIYDLFVPVSSKLQKRKMLRLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGVKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPRVTRVSELSLCDLAGSERSMKTQSEGERLREAGNINTSLLTLGKCISVLKNSDKSKIQQHVPFRESKLTHYFQSFFTGKGKICMIINISQCCSAYDETLNVLKFSTVAQKVYVPDTLSSSQEKSFGSTKSLQDVSLGSNLDNKILNVKRKTVSWENSLEDVVENEDLVEDLEENEETQNMETELTDEDSDKPLEEGGVCAGHGKNKKLLDLIENLKKRLINEKKEKLTLEFKIREEVTQEFTQYWSQREADFKETLLHEREILEENAERRLAIFKDLVGKPGESQDEPASRFCTMELETEESHNYVGVEDIFDSLEDDVTDIKKQAELAHLYITSLVDPQEAIACLQLKYNQVKAELAETKEELIKAQEELKNKESDSLVQALKTSSKVDTSLISNKSTGNETTEMPKKSRTQTHSERKRLNEDGLQLGEPPAKKGLILISPPITEDQDKREEMQQSVSEGAEEDSRVLQEKNEELKRLLTIGENELRNAKEEKAELNKQVVSLQQQLCFFEEKNSSLRAEVEQIQASYDLAAAELHTQRAVNQEQKDRILQLSGKMETAARRIESNVSQIKQMQTKIDELRSLDSPSHISKIDLLNLQDLSSGANLLNTSQQLPGSDLPSTWVKEFHTQELSRESSFHSSIEAIWEECKEIVKASSKKSHQIQGLEELIEKLQVEVKNCRDENSELRAKESEDKNRDQQLKEKESLIQQLREELQETTVSLRVQVQLVAEREQALSELSRDVTCYKAKVKDLEVMVETQKEECKRLAELEQSILEKESAILKLEASLKELEAKHQDHIRSTTHLNAEEVKFREEITQLANNLHDTKQLLQSKEEENEISRQETEKLKEELAANSVLTQNLQADLQRKEEDCAELKEKFTDAKKQIEQVQREVSVMRDEEKSLRTKINELEKKKNQYSQEIDMKQRTIQQLKEQLSNQKMEEVVQQYEKVCKDLSVKEKLIEAMRLTLVEQEQTQAEQDRMLEAKSQEADWLAGELDTWKDKFKDLETRSNQKVTTEAMEDSDVLSEKFRKLQDELQESEEKHKADRKKWLEEKAVLTTQAKEAETLRNREMKKYAEDRERCLKLQNEVETLTAQLAEKTGELQKWREERDQLVTAVETQMQALLSSSKHKDEEIQQLRKAVAKSTGTVSGRVLENQTMNLKPECNDSVDLGGVETELQSTSFEISRNTAEDGSVVLDSCEVSTENVQSTRFPKPELEIQFTPLQPNKVAVKHPGCATPVTIKIPKARKRKSGEVEEDLVKCENKKNSTPRSNVKFPVSEHRNSSFKKEQKVFLCPSSKKTYSLRSQASTVGANVASKKKEGTLQKFGDFLQHSPTILQSKAKKIIETMSSPKLSTVEVSKENVSQPKKAKRKLYRNEISSPINISGQVILMEQKAKETDHQILKRRLRTRTAK

Target ORF information:

RefSeq Version	XM_006231281.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231281.2

ORF Insert Sequence:

ATGGAATCTC ATTTGAATCA AGACGGAGTG CCTAGACCAT CTTATGTTTT TAGTGCTGAC 
CCAATTGCAA GGCCTTCGGA AATCAATTTT GATGGCGTTA AGCTTGATCT CTCTCATGAG 
TTTTCCTTAG TTGCCGCAAA CCCTGGGGCA AACAGTTTGG AATCTAAAAA TTACCTCCAG 
GTTTGTCTTC GAATAAGACC ATTTACACAG TCGGAAAAAG GACACGAGGC TGAGGGCTGT 
GTGCAAGTTC TGGATTCACA GACTGTTCTG CTGAAAGACC CTCAAAGCAT CCTTGGCCAC 
TTAAGTGAGA AGAGCTCCGG ACAGATGGCA CAGAAATTCA GCTTTTCTAG GGTTTTTGGA 
CCCGAAACTT CACAGAAAGA GTTCTTTCAG GGTTGCATTA TGCAGCCAGT AAAAGACCTC 
TTAAAAGGGC ACAGTCGACT GATCTTCACA TATGGGCTGA CCAATTCTGG AAAAACATAT 
ACATTTCAAG GCACAGAAGA AAACATTGGC ATTCTGCCTA GAACACTGAA TGTATTGTTT 
GATAGTCTTC AGGAAAGACT CTATACAAAG ATGAGCTTCA AACCACATAG ATGTAGAGAA 
TACCTACAGT TGTCATCTGA TCAAGAGAAA GAGGAAAGTG CTAACAAAAA TACATTGCTG 
CGACAAATTA AAGAGGTTAC CATACATAAT GACAGTTATG ACATTCTTTG TGGACGCTTA 
ACAAACTCTT TGACTATACC AGAATTTGAA GAAACCATGA ACAATTGTGA ACAATCTAGT 
CTCAATGTGG ATAATATAAA ATATTCAGTG TGGGTTTCTT TCTTTGAGAT TTACAATGAA 
AGTATTTATG ATTTGTTTGT TCCTGTGTCC TCTAAGTTGC AAAAGAGAAA AATGCTACGC 
CTTTCCCAAG ATGTAAAGGG CTATTCTTTT ATAAAAGATC TGCAGTGGAT CCAGGTATCT 
GATTCTAAAG AAGCATATAG ACTCTTAAAA CTAGGAGTGA AGCATCAGAG TGTTGCCTTC 
ACAAAACTGA ACAATGCTTC TAGCAGAAGT CACAGCATAT TCACTATTAG AATATTACAG 
ATAGAAGACT CGGAAATACC TCGTGTAACA CGAGTTAGTG AATTGTCGCT ATGTGATCTC 
GCTGGTTCAG AACGAAGCAT GAAGACGCAA AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG 
AATATCAACA CTTCTTTACT GACTCTGGGA AAGTGCATCA GTGTTCTGAA GAACAGTGAC 
AAGTCCAAGA TTCAACAACA TGTGCCTTTT CGAGAAAGTA AACTGACCCA CTATTTCCAA 
AGCTTTTTTA CTGGAAAGGG GAAAATATGT ATGATCATCA ATATTAGTCA GTGTTGTTCT 
GCCTATGATG AGACACTCAA TGTACTGAAG TTCTCAACTG TTGCACAAAA GGTCTATGTT 
CCAGATACTT TAAGTTCTTC TCAAGAGAAA TCGTTTGGGT CTACCAAATC TTTACAAGAT 
GTATCCCTAG GCAGTAATTT GGATAATAAA ATACTAAATG TAAAAAGAAA AACTGTTTCA 
TGGGAAAATA GTCTGGAAGA TGTGGTTGAA AATGAAGATT TGGTCGAGGA CTTAGAAGAA 
AATGAAGAAA CACAAAACAT GGAAACTGAA CTTACAGATG AAGATAGTGA TAAGCCGTTA 
GAGGAAGGCG GAGTCTGCGC AGGTCACGGG AAGAACAAGA AACTGTTGGA TTTAATAGAA 
AACTTGAAAA AAAGACTAAT AAATGAAAAA AAAGAAAAGT TAACATTGGA ATTTAAAATC 
CGAGAAGAAG TGACACAGGA ATTCACTCAG TATTGGAGCC AAAGGGAAGC TGATTTTAAG 
GAAACACTTC TTCATGAACG GGAGATATTA GAAGAAAATG CTGAACGTCG CTTAGCAATT 
TTCAAAGATT TGGTTGGAAA ACCTGGTGAG AGTCAAGATG AACCAGCAAG CAGATTTTGT 
ACCATGGAAC TTGAAACTGA AGAATCTCAT AATTATGTAG GAGTTGAAGA TATTTTTGAT 
TCTCTTGAAG ATGATGTCAC TGATATTAAG AAACAGGCCG AACTTGCTCA TTTGTATATT 
ACATCTCTTG TTGATCCCCA GGAGGCTATT GCTTGTTTGC AGCTAAAGTA TAATCAAGTT 
AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA TTAATTAAAG CCCAAGAAGA GCTAAAAAAT 
AAAGAGAGTG ACTCTCTGGT TCAGGCACTC AAGACATCAA GTAAGGTTGA TACATCTTTA 
ATCAGCAATA AGTCCACTGG TAATGAAACA ACTGAAATGC CCAAGAAGAG TAGAACCCAG 
ACACATTCAG AAAGGAAAAG ATTAAATGAA GATGGACTTC AGCTTGGTGA ACCACCAGCA 
AAGAAAGGGC TTATACTTAT TAGTCCACCC ATCACTGAAG ACCAAGACAA AAGGGAAGAA 
ATGCAACAGT CTGTTTCAGA AGGTGCAGAA GAAGACAGTA GAGTTTTGCA GGAAAAGAAT 
GAAGAGCTAA AAAGACTTTT AACTATTGGT GAGAATGAAC TCAGAAATGC AAAGGAGGAA 
AAGGCAGAAT TAAATAAACA GGTTGTGAGT TTGCAGCAGC AACTTTGTTT CTTTGAAGAA 
AAGAACTCAT CCCTGAGGGC AGAGGTAGAG CAAATCCAGG CCAGTTATGA CCTTGCAGCT 
GCTGAGCTGC ATACTCAGAG AGCTGTGAAC CAGGAGCAGA AGGACAGGAT CCTGCAATTG 
TCAGGGAAGA TGGAAACTGC GGCAAGACGT ATTGAAAGCA ATGTTTCACA GATAAAGCAA 
ATGCAAACAA AAATAGATGA ACTGCGATCT CTTGATTCAC CTTCTCATAT TTCAAAAATA 
GATTTACTCA ATCTCCAGGA TCTATCAAGT GGTGCTAATT TGCTGAACAC ATCACAACAG 
CTTCCAGGTA GTGACCTACC GAGCACATGG GTTAAAGAGT TTCATACTCA GGAGCTCAGC 
AGGGAAAGTT CCTTCCACTC TAGCATTGAA GCCATCTGGG AAGAATGTAA AGAGATTGTG 
AAGGCTTCCT CCAAAAAGTC TCATCAGATT CAGGGACTAG AAGAACTAAT TGAAAAATTA 
CAGGTGGAAG TAAAAAACTG CAGGGATGAG AACAGTGAGC TTCGAGCAAA GGAGAGTGAG 
GATAAGAATC GAGACCAGCA GCTGAAGGAG AAAGAAAGTC TAATCCAGCA GCTTAGAGAA 
GAACTTCAGG AAACCACTGT CAGTCTCCGT GTCCAAGTAC AGCTAGTAGC GGAAAGAGAG 
CAAGCTCTTT CAGAGCTTTC CCGGGATGTT ACCTGCTATA AGGCAAAAGT AAAGGACCTT 
GAAGTGATGG TAGAGACCCA GAAAGAGGAA TGTAAGCGCT TAGCTGAGTT AGAGCAGAGC 
ATTTTAGAGA AGGAATCTGC CATCCTAAAG CTGGAAGCGA GTCTAAAGGA ATTGGAAGCA 
AAGCACCAGG ATCACATCAG AAGTACCACA CATTTAAATG CAGAGGAAGT CAAGTTCAGA 
GAAGAAATTA CACAGTTGGC GAATAATTTG CATGATACAA AACAGTTACT TCAGTCAAAG 
GAAGAAGAAA ATGAAATTAG CAGACAAGAG ACAGAAAAGT TGAAAGAGGA GCTTGCTGCA 
AACTCTGTTC TTACCCAGAA TCTCCAAGCA GACCTTCAGC GGAAGGAGGA AGATTGTGCT 
GAGCTAAAGG AGAAGTTCAC TGATGCCAAA AAGCAGATCG AGCAGGTACA GAGGGAGGTA 
TCTGTAATGC GTGATGAAGA GAAGTCGTTG AGGACTAAAA TTAATGAACT GGAGAAAAAG 
AAAAACCAAT ATTCTCAGGA AATAGATATG AAACAACGAA CCATTCAGCA ACTTAAGGAG 
CAGTTAAGTA ATCAGAAAAT GGAAGAAGTT GTACAACAGT ATGAGAAAGT GTGCAAAGAT 
CTCAGCGTTA AGGAGAAGCT GATTGAAGCT ATGCGACTGA CACTAGTGGA GCAGGAGCAA 
ACTCAGGCAG AGCAGGACAG GATGCTTGAG GCTAAGTCAC AGGAAGCTGA CTGGCTAGCC 
GGAGAATTGG ATACATGGAA AGACAAATTC AAAGATCTGG AGACTAGAAG TAATCAAAAG 
GTAACTACAG AGGCCATGGA GGACAGCGAC GTGCTTAGTG AGAAGTTCAG GAAGCTTCAG 
GACGAGCTGC AGGAATCTGA AGAGAAACAT AAAGCTGATA GAAAGAAGTG GTTAGAAGAA 
AAAGCGGTTC TTACAACTCA AGCAAAAGAA GCAGAGACTC TACGAAACAG GGAGATGAAA 
AAATATGCTG AGGACAGGGA GCGTTGTCTG AAGCTGCAGA ATGAAGTGGA AACACTGACA 
GCACAGCTGG CGGAGAAAAC TGGTGAGCTT CAGAAGTGGA GAGAAGAGCG GGACCAGCTG 
GTCACAGCTG TGGAGACACA GATGCAGGCT CTGCTCTCCA GCAGCAAGCA TAAGGACGAA 
GAGATCCAGC AACTGAGAAA GGCCGTGGCA AAGAGCACGG GGACAGTGAG TGGACGCGTA 
CTCGAAAACC AGACCATGAA TCTCAAGCCT GAATGTAATG ATTCAGTCGA TCTTGGTGGA 
GTTGAAACTG AACTTCAGTC AACAAGTTTT GAAATTTCTA GGAATACAGC AGAGGATGGA 
TCTGTAGTTC TTGACTCTTG TGAAGTGTCA ACAGAAAATG TACAAAGTAC TCGATTTCCA 
AAGCCTGAAC TGGAGATTCA GTTTACACCT CTGCAGCCAA ACAAAGTGGC AGTGAAGCAC 
CCTGGTTGTG CCACACCAGT CACAATTAAG ATCCCCAAGG CACGGAAGAG GAAGAGTGGC 
GAGGTGGAGG AGGACTTGGT GAAATGTGAA AATAAGAAGA ATTCCACACC CAGAAGTAAT 
GTGAAGTTTC CCGTTTCAGA GCATAGAAAT TCCTCTTTCA AAAAGGAACA AAAGGTTTTC 
TTATGTCCGT CATCTAAGAA GACATACTCC TTACGGAGCC AAGCATCTAC GGTTGGTGCG 
AATGTAGCCT CTAAAAAAAA AGAGGGAACA CTACAGAAAT TTGGAGACTT CTTACAACAT 
TCTCCAACAA TCCTTCAATC AAAAGCAAAA AAGATAATTG AAACAATGAG TTCCCCAAAG 
TTATCAACTG TAGAAGTAAG TAAAGAAAAT GTATCCCAGC CTAAAAAAGC CAAACGGAAA 
TTGTACAGAA ATGAAATTTC ATCTCCCATC AACATATCTG GCCAAGTGAT TTTAATGGAA 
CAGAAAGCAA AAGAAACTGA TCATCAGATT CTCAAACGAC GGCTTCGAAC ACGAACAGCC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif20b ( XM_006231283.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231345.1 Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X4, mRNA.

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CloneID	ORa24036
Clone ID Related Accession (Same CDS sequence)	XM_006231283.2
Accession Version	XM_006231283.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF20B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006231283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTC ATTTGAATCA AGACGGAGTG CCTAGACCAT CTTATGTTTT TAGTGCTGAC 
CCAATTGCAA GGCCTTCGGA AATCAATTTT GATGGCGTTA AGCTTGATCT CTCTCATGAG 
TTTTCCTTAG TTGCCGCAAA CCCTGGGGCA AACAGTTTGG AATCTAAAAA TTACCTCCAG 
GTTTGTCTTC GAATAAGACC ATTTACACAG TCGGAAAAAG GACACGAGGC TGAGGGCTGT 
GTGCAAGTTC TGGATTCACA GACTGTTCTG CTGAAAGACC CTCAAAGCAT CCTTGGCCAC 
TTAAGTGAGA AGAGCTCCGG ACAGATGGCA CAGAAATTCA GCTTTTCTAG GGTTTTTGGA 
CCCGAAACTT CACAGAAAGA GTTCTTTCAG GGTTGCATTA TGCAGCCAGT AAAAGACCTC 
TTAAAAGGGC ACAGTCGACT GATCTTCACA TATGGGCTGA CCAATTCTGG AAAAACATAT 
ACATTTCAAG GCACAGAAGA AAACATTGGC ATTCTGCCTA GAACACTGAA TGTATTGTTT 
GATAGTCTTC AGGAAAGACT CTATACAAAG ATGAGCTTCA AACCACATAG ATGTAGAGAA 
TACCTACAGT TGTCATCTGA TCAAGAGAAA GAGGAAAGTG CTAACAAAAA TACATTGCTG 
CGACAAATTA AAGAGGTTAC CATACATAAT GACAGTTATG ACATTCTTTG TGGACGCTTA 
ACAAACTCTT TGACTATACC AGAATTTGAA GAAACCATGA ACAATTGTGA ACAATCTAGT 
CTCAATGTGG ATAATATAAA ATATTCAGTG TGGGTTTCTT TCTTTGAGAT TTACAATGAA 
AGTATTTATG ATTTGTTTGT TCCTGTGTCC TCTAAGTTGC AAAAGAGAAA AATGCTACGC 
CTTTCCCAAG ATGTAAAGGG CTATTCTTTT ATAAAAGATC TGCAGTGGAT CCAGGTATCT 
GATTCTAAAG AAGCATATAG ACTCTTAAAA CTAGGAGTGA AGCATCAGAG TGTTGCCTTC 
ACAAAACTGA ACAATGCTTC TAGCAGAAGT CACAGCATAT TCACTATTAG AATATTACAG 
ATAGAAGACT CGGAAATACC TCGTGTAACA CGAGTTAGTG AATTGTCGCT ATGTGATCTC 
GCTGGTTCAG AACGAAGCAT GAAGACGCAA AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG 
AATATCAACA CTTCTTTACT GACTCTGGGA AAGTGCATCA GTGTTCTGAA GAACAGTGAC 
AAGTCCAAGA TTCAACAACA TGTGCCTTTT CGAGAAAGTA AACTGACCCA CTATTTCCAA 
AGCTTTTTTA CTGGAAAGGG GAAAATATGT ATGATCATCA ATATTAGTCA GTGTTGTTCT 
GCCTATGATG AGACACTCAA TGTACTGAAG TTCTCAACTG TTGCACAAAA GGTCTATGTT 
CCAGATACTT TAAGTTCTTC TCAAGAGAAA TCGTTTGGGT CTACCAAATC TTTACAAGAT 
GTATCCCTAG GCAGTAATTT GGATAATAAA ATACTAAATG TAAAAAGAAA AACTGTTTCA 
TGGGAAAATA GTCTGGAAGA TGTGGTTGAA AATGAAGATT TGGTCGAGGA CTTAGAAGAA 
AATGAAGAAA CACAAAACAT GGAAACTGAA CTTACAGATG AAGATAGTGA TAAGCCGTTA 
GAGGAAGGCG GAGTCTGCGC AGGTCACGGG AAGAACAAGA AACTGTTGGA TTTAATAGAA 
AACTTGAAAA AAAGACTAAT AAATGAAAAA AAAGAAAAGT TAACATTGGA ATTTAAAATC 
CGAGAAGAAG TGACACAGGA ATTCACTCAG TATTGGAGCC AAAGGGAAGC TGATTTTAAG 
GAAACACTTC TTCATGAACG GGAGATATTA GAAGAAAATG CTGAACGTCG CTTAGCAATT 
TTCAAAGATT TGGTTGGAAA ACCTGGTGAG AGTCAAGATG AACCAGCAAG CAGATTTTGT 
ACCATGGAAC TTGAAACTGA AGAATCTCAT AATTATGTAG GAGTTGAAGA TATTTTTGAT 
TCTCTTGAAG ATGATGTCAC TGATATTAAG AAACAGGCCG AACTTGCTCA TTTGTATATT 
ACATCTCTTG TTGATCCCCA GGAGGCTATT GCTTGTTTGC AGCTAAAGTA TAATCAAGTT 
AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA TTAATTAAAG CCCAAGAAGA GCTAAAAAAT 
AAAGAGAGTG ACTCTCTGGT TCAGGCACTC AAGACATCAA GTAAGGTTGA TACATCTTTA 
ATCAGCAATA AGTCCACTGG TAATGAAACA ACTGAAATGC CCAAGAAGAG TAGAACCCAG 
ACACATTCAG AAAGGAAAAG ATTAAATGAA GATGGACTTC AGCTTGGTGA ACCACCAGCA 
AAGAAAGGGC TTATACTTAT TAGTCCACCC ATCACTGAAG ACCAAGACAA AAGGGAAGAA 
ATGCAACAGT CTGTTTCAGA AGGTGCAGAA GAAGACAGTA GAGTTTTGCA GGAAAAGAAT 
GAAGAGCTAA AAAGACTTTT AACTATTGGT GAGAATGAAC TCAGAAATGC AAAGGAGGAA 
AAGGCAGAAT TAAATAAACA GGTTGTGAGT TTGCAGCAGC AACTTTGTTT CTTTGAAGAA 
AAGAACTCAT CCCTGAGGGC AGAGGTAGAG CAAATCCAGG CCAGTTATGA CCTTGCAGCT 
GCTGAGCTGC ATACTCAGAG AGCTGTGAAC CAGGAGCAGA AGGACAGGAT CCTGCAATTG 
TCAGGGAAGA TGGAAACTGC GGCAAGACGT ATTGAAAGCA ATGTTTCACA GATAAAGCAA 
ATGCAAACAA AAATAGATGA ACTGCGATCT CTTGATTCAC CTTCTCATAT TTCAAAAATA 
GATTTACTCA ATCTCCAGGA TCTATCAAGT GGTGCTAATT TGCTGAACAC ATCACAACAG 
CTTCCAGGTA GTGACCTACC GAGCACATGG GTTAAAGAGT TTCATACTCA GGAGCTCAGC 
AGGGAAAGTT CCTTCCACTC TAGCATTGAA GCCATCTGGG AAGAATGTAA AGAGATTGTG 
AAGGCTTCCT CCAAAAAGTC TCATCAGATT CAGGGACTAG AAGAACTAAT TGAAAAATTA 
CAGGTGGAAG TAAAAAACTG CAGGGATGAG AACAGTGAGC TTCGAGCAAA GGAGAGTGAG 
GATAAGAATC GAGACCAGCA GCTGAAGGAG AAAGAAAGTC TAATCCAGCA GCTTAGAGAA 
GAACTTCAGG AAACCACTGT CAGTCTCCGT GTCCAAGTAC AGCTAGTAGC GGAAAGAGAG 
CAAGCTCTTT CAGAGCTTTC CCGGGATGTT ACCTGCTATA AGGCAAAAGT AAAGGACCTT 
GAAGTGATGG TAGAGACCCA GAAAGAGGAA TGTAAGCGCT TAGCTGAGTT AGAGCAGAGC 
ATTTTAGAGA AGGAATCTGC CATCCTAAAG CTGGAAGCGA GTCTAAAGGA ATTGGAAGCA 
AAGCACCAGG ATCACATCAG AAGTACCACA CATTTAAATG CAGAGGAAGT CAAGTTCAGA 
GAAGAAATTA CACAGTTGGC GAATAATTTG CATGATACAA AACAGTTACT TCAGTCAAAG 
GAAGAAGAAA ATGAAATTAG CAGACAAGAG ACAGAAAAGT TGAAAGAGGA GCTTGCTGCA 
AACTCTGTTC TTACCCAGAA TCTCCAAGCA GACCTTCAGC GGAAGGAGGA AGATTGTGCT 
GAGCTAAAGG AGAAGTTCAC TGATGCCAAA AAGCAGATCG AGCAGGTACA GAGGGAGGTA 
TCTGTAATGC GTGATGAAGA GAAGTCGTTG AGGACTAAAA TTAATGAACT GGAGAAAAAG 
AAAAACCAAT ATTCTCAGGA AATAGATATG AAACAACGAA CCATTCAGCA ACTTAAGGAG 
CAGTTAAGTA ATCAGAAAAT GGAAGAAGTT GTACAACAGT ATGAGAAAGT GTGCAAAGAT 
CTCAGCGTTA AGGAGAAGCT GATTGAAGCT ATGCGACTGA CACTAGTGGA GCAGGAGCAA 
ACTCAGGCAG AGCAGGACAG GATGCTTGAG GCTAAGTCAC AGGAAGCTGA CTGGCTAGCC 
GGAGAATTGG ATACATGGAA AGACAAATTC AAAGATCTGG AGACTAGAAG TAATCAAAAG 
GTAACTACAG AGGCCATGGA GGACAGCGAC GTGCTTAGTG AGAAGTTCAG GAAGCTTCAG 
GACGAGCTGC AGGAATCTGA AGAGAAACAT AAAGCTGATA GAAAGAAGTG GTTAGAAGAA 
AAAGCGGTTC TTACAACTCA AGCAAAAGAA GCAGAGACTC TACGAAACAG GGAGATGAAA 
AAATATGCTG AGGACAGGGA GCGTTGTCTG AAGCTGCAGA ATGAAGTGGA AACACTGACA 
GCACAGCTGG CGGAGAAAAC TGGTGAGCTT CAGAAGTGGA GAGAAGAGCG GGACCAGCTG 
GTCACAGCTG TGGAGACACA GATGCAGGCT CTGCTCTCCA GCAGCAAGCA TAAGGACGAA 
GAGATCCAGC AACTGAGAAA GGCCGTGGCA AAGAGCACGG GGACAGAAAA CCAGACCATG 
AATCTCAAGC CTGAATGTAA TGATTCAGTC GATCTTGGTG GAGTTGAAAC TGAACTTCAG 
TCAACAAGTT TTGAAATTTC TAGGAATACA GCAGAGGATG GATCTGTAGT TCTTGACTCT 
TGTGAAGTGT CAACAGAAAA TGTACAAAGT ACTCGATTTC CAAAGCCTGA ACTGGAGATT 
CAGTTTACAC CTCTGCAGCC AAACAAAGTG GCAGTGAAGC ACCCTGGTTG TGCCACACCA 
GTCACAATTA AGATCCCCAA GGCACGGAAG AGGAAGAGTG GCGAGGTGGA GGAGGACTTG 
GTGAAATGTG AAAATAAGAA GAATTCCACA CCCAGAAGTA ATGTGAAGTT TCCCGTTTCA 
GAGCATAGAA ATTCCTCTTT CAAAAAGGAA CAAAAGGTTT TCTTATGTCC GTCATCTAAG 
AAGACATACT CCTTACGGAG CCAAGCATCT ACGGTTGGTG CGAATGTAGC CTCTAAAAAA 
AAAGAGGGAA CACTACAGAA ATTTGGAGAC TTCTTACAAC ATTCTCCAAC AATCCTTCAA 
TCAAAAGCAA AAAAGATAAT TGAAACAATG AGTTCCCCAA AGTTATCAAC TGTAGAAGTA 
AGTAAAGAAA ATGTATCCCA GCCTAAAAAA GCCAAACGGA AATTGTACAG AAATGAAATT 
TCATCTCCCA TCAACATATC TGGCCAAGTG ATTTTAATGG AACAGAAAGC AAAAGAAACT 
GATCATCAGA TTCTCAAACG ACGGCTTCGA ACACGAACAG CCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231345.1
CDS	120..5447
Translation	MESHLNQDGVPRPSYVFSADPIARPSEINFDGVKLDLSHEFSLVAANPGANSLESKNYLQVCLRIRPFTQSEKGHEAEGCVQVLDSQTVLLKDPQSILGHLSEKSSGQMAQKFSFSRVFGPETSQKEFFQGCIMQPVKDLLKGHSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLFDSLQERLYTKMSFKPHRCREYLQLSSDQEKEESANKNTLLRQIKEVTIHNDSYDILCGRLTNSLTIPEFEETMNNCEQSSLNVDNIKYSVWVSFFEIYNESIYDLFVPVSSKLQKRKMLRLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGVKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPRVTRVSELSLCDLAGSERSMKTQSEGERLREAGNINTSLLTLGKCISVLKNSDKSKIQQHVPFRESKLTHYFQSFFTGKGKICMIINISQCCSAYDETLNVLKFSTVAQKVYVPDTLSSSQEKSFGSTKSLQDVSLGSNLDNKILNVKRKTVSWENSLEDVVENEDLVEDLEENEETQNMETELTDEDSDKPLEEGGVCAGHGKNKKLLDLIENLKKRLINEKKEKLTLEFKIREEVTQEFTQYWSQREADFKETLLHEREILEENAERRLAIFKDLVGKPGESQDEPASRFCTMELETEESHNYVGVEDIFDSLEDDVTDIKKQAELAHLYITSLVDPQEAIACLQLKYNQVKAELAETKEELIKAQEELKNKESDSLVQALKTSSKVDTSLISNKSTGNETTEMPKKSRTQTHSERKRLNEDGLQLGEPPAKKGLILISPPITEDQDKREEMQQSVSEGAEEDSRVLQEKNEELKRLLTIGENELRNAKEEKAELNKQVVSLQQQLCFFEEKNSSLRAEVEQIQASYDLAAAELHTQRAVNQEQKDRILQLSGKMETAARRIESNVSQIKQMQTKIDELRSLDSPSHISKIDLLNLQDLSSGANLLNTSQQLPGSDLPSTWVKEFHTQELSRESSFHSSIEAIWEECKEIVKASSKKSHQIQGLEELIEKLQVEVKNCRDENSELRAKESEDKNRDQQLKEKESLIQQLREELQETTVSLRVQVQLVAEREQALSELSRDVTCYKAKVKDLEVMVETQKEECKRLAELEQSILEKESAILKLEASLKELEAKHQDHIRSTTHLNAEEVKFREEITQLANNLHDTKQLLQSKEEENEISRQETEKLKEELAANSVLTQNLQADLQRKEEDCAELKEKFTDAKKQIEQVQREVSVMRDEEKSLRTKINELEKKKNQYSQEIDMKQRTIQQLKEQLSNQKMEEVVQQYEKVCKDLSVKEKLIEAMRLTLVEQEQTQAEQDRMLEAKSQEADWLAGELDTWKDKFKDLETRSNQKVTTEAMEDSDVLSEKFRKLQDELQESEEKHKADRKKWLEEKAVLTTQAKEAETLRNREMKKYAEDRERCLKLQNEVETLTAQLAEKTGELQKWREERDQLVTAVETQMQALLSSSKHKDEEIQQLRKAVAKSTGTENQTMNLKPECNDSVDLGGVETELQSTSFEISRNTAEDGSVVLDSCEVSTENVQSTRFPKPELEIQFTPLQPNKVAVKHPGCATPVTIKIPKARKRKSGEVEEDLVKCENKKNSTPRSNVKFPVSEHRNSSFKKEQKVFLCPSSKKTYSLRSQASTVGANVASKKKEGTLQKFGDFLQHSPTILQSKAKKIIETMSSPKLSTVEVSKENVSQPKKAKRKLYRNEISSPINISGQVILMEQKAKETDHQILKRRLRTRTAK

Target ORF information:

RefSeq Version	XM_006231283.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231283.2

ORF Insert Sequence:

ATGGAATCTC ATTTGAATCA AGACGGAGTG CCTAGACCAT CTTATGTTTT TAGTGCTGAC 
CCAATTGCAA GGCCTTCGGA AATCAATTTT GATGGCGTTA AGCTTGATCT CTCTCATGAG 
TTTTCCTTAG TTGCCGCAAA CCCTGGGGCA AACAGTTTGG AATCTAAAAA TTACCTCCAG 
GTTTGTCTTC GAATAAGACC ATTTACACAG TCGGAAAAAG GACACGAGGC TGAGGGCTGT 
GTGCAAGTTC TGGATTCACA GACTGTTCTG CTGAAAGACC CTCAAAGCAT CCTTGGCCAC 
TTAAGTGAGA AGAGCTCCGG ACAGATGGCA CAGAAATTCA GCTTTTCTAG GGTTTTTGGA 
CCCGAAACTT CACAGAAAGA GTTCTTTCAG GGTTGCATTA TGCAGCCAGT AAAAGACCTC 
TTAAAAGGGC ACAGTCGACT GATCTTCACA TATGGGCTGA CCAATTCTGG AAAAACATAT 
ACATTTCAAG GCACAGAAGA AAACATTGGC ATTCTGCCTA GAACACTGAA TGTATTGTTT 
GATAGTCTTC AGGAAAGACT CTATACAAAG ATGAGCTTCA AACCACATAG ATGTAGAGAA 
TACCTACAGT TGTCATCTGA TCAAGAGAAA GAGGAAAGTG CTAACAAAAA TACATTGCTG 
CGACAAATTA AAGAGGTTAC CATACATAAT GACAGTTATG ACATTCTTTG TGGACGCTTA 
ACAAACTCTT TGACTATACC AGAATTTGAA GAAACCATGA ACAATTGTGA ACAATCTAGT 
CTCAATGTGG ATAATATAAA ATATTCAGTG TGGGTTTCTT TCTTTGAGAT TTACAATGAA 
AGTATTTATG ATTTGTTTGT TCCTGTGTCC TCTAAGTTGC AAAAGAGAAA AATGCTACGC 
CTTTCCCAAG ATGTAAAGGG CTATTCTTTT ATAAAAGATC TGCAGTGGAT CCAGGTATCT 
GATTCTAAAG AAGCATATAG ACTCTTAAAA CTAGGAGTGA AGCATCAGAG TGTTGCCTTC 
ACAAAACTGA ACAATGCTTC TAGCAGAAGT CACAGCATAT TCACTATTAG AATATTACAG 
ATAGAAGACT CGGAAATACC TCGTGTAACA CGAGTTAGTG AATTGTCGCT ATGTGATCTC 
GCTGGTTCAG AACGAAGCAT GAAGACGCAA AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG 
AATATCAACA CTTCTTTACT GACTCTGGGA AAGTGCATCA GTGTTCTGAA GAACAGTGAC 
AAGTCCAAGA TTCAACAACA TGTGCCTTTT CGAGAAAGTA AACTGACCCA CTATTTCCAA 
AGCTTTTTTA CTGGAAAGGG GAAAATATGT ATGATCATCA ATATTAGTCA GTGTTGTTCT 
GCCTATGATG AGACACTCAA TGTACTGAAG TTCTCAACTG TTGCACAAAA GGTCTATGTT 
CCAGATACTT TAAGTTCTTC TCAAGAGAAA TCGTTTGGGT CTACCAAATC TTTACAAGAT 
GTATCCCTAG GCAGTAATTT GGATAATAAA ATACTAAATG TAAAAAGAAA AACTGTTTCA 
TGGGAAAATA GTCTGGAAGA TGTGGTTGAA AATGAAGATT TGGTCGAGGA CTTAGAAGAA 
AATGAAGAAA CACAAAACAT GGAAACTGAA CTTACAGATG AAGATAGTGA TAAGCCGTTA 
GAGGAAGGCG GAGTCTGCGC AGGTCACGGG AAGAACAAGA AACTGTTGGA TTTAATAGAA 
AACTTGAAAA AAAGACTAAT AAATGAAAAA AAAGAAAAGT TAACATTGGA ATTTAAAATC 
CGAGAAGAAG TGACACAGGA ATTCACTCAG TATTGGAGCC AAAGGGAAGC TGATTTTAAG 
GAAACACTTC TTCATGAACG GGAGATATTA GAAGAAAATG CTGAACGTCG CTTAGCAATT 
TTCAAAGATT TGGTTGGAAA ACCTGGTGAG AGTCAAGATG AACCAGCAAG CAGATTTTGT 
ACCATGGAAC TTGAAACTGA AGAATCTCAT AATTATGTAG GAGTTGAAGA TATTTTTGAT 
TCTCTTGAAG ATGATGTCAC TGATATTAAG AAACAGGCCG AACTTGCTCA TTTGTATATT 
ACATCTCTTG TTGATCCCCA GGAGGCTATT GCTTGTTTGC AGCTAAAGTA TAATCAAGTT 
AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA TTAATTAAAG CCCAAGAAGA GCTAAAAAAT 
AAAGAGAGTG ACTCTCTGGT TCAGGCACTC AAGACATCAA GTAAGGTTGA TACATCTTTA 
ATCAGCAATA AGTCCACTGG TAATGAAACA ACTGAAATGC CCAAGAAGAG TAGAACCCAG 
ACACATTCAG AAAGGAAAAG ATTAAATGAA GATGGACTTC AGCTTGGTGA ACCACCAGCA 
AAGAAAGGGC TTATACTTAT TAGTCCACCC ATCACTGAAG ACCAAGACAA AAGGGAAGAA 
ATGCAACAGT CTGTTTCAGA AGGTGCAGAA GAAGACAGTA GAGTTTTGCA GGAAAAGAAT 
GAAGAGCTAA AAAGACTTTT AACTATTGGT GAGAATGAAC TCAGAAATGC AAAGGAGGAA 
AAGGCAGAAT TAAATAAACA GGTTGTGAGT TTGCAGCAGC AACTTTGTTT CTTTGAAGAA 
AAGAACTCAT CCCTGAGGGC AGAGGTAGAG CAAATCCAGG CCAGTTATGA CCTTGCAGCT 
GCTGAGCTGC ATACTCAGAG AGCTGTGAAC CAGGAGCAGA AGGACAGGAT CCTGCAATTG 
TCAGGGAAGA TGGAAACTGC GGCAAGACGT ATTGAAAGCA ATGTTTCACA GATAAAGCAA 
ATGCAAACAA AAATAGATGA ACTGCGATCT CTTGATTCAC CTTCTCATAT TTCAAAAATA 
GATTTACTCA ATCTCCAGGA TCTATCAAGT GGTGCTAATT TGCTGAACAC ATCACAACAG 
CTTCCAGGTA GTGACCTACC GAGCACATGG GTTAAAGAGT TTCATACTCA GGAGCTCAGC 
AGGGAAAGTT CCTTCCACTC TAGCATTGAA GCCATCTGGG AAGAATGTAA AGAGATTGTG 
AAGGCTTCCT CCAAAAAGTC TCATCAGATT CAGGGACTAG AAGAACTAAT TGAAAAATTA 
CAGGTGGAAG TAAAAAACTG CAGGGATGAG AACAGTGAGC TTCGAGCAAA GGAGAGTGAG 
GATAAGAATC GAGACCAGCA GCTGAAGGAG AAAGAAAGTC TAATCCAGCA GCTTAGAGAA 
GAACTTCAGG AAACCACTGT CAGTCTCCGT GTCCAAGTAC AGCTAGTAGC GGAAAGAGAG 
CAAGCTCTTT CAGAGCTTTC CCGGGATGTT ACCTGCTATA AGGCAAAAGT AAAGGACCTT 
GAAGTGATGG TAGAGACCCA GAAAGAGGAA TGTAAGCGCT TAGCTGAGTT AGAGCAGAGC 
ATTTTAGAGA AGGAATCTGC CATCCTAAAG CTGGAAGCGA GTCTAAAGGA ATTGGAAGCA 
AAGCACCAGG ATCACATCAG AAGTACCACA CATTTAAATG CAGAGGAAGT CAAGTTCAGA 
GAAGAAATTA CACAGTTGGC GAATAATTTG CATGATACAA AACAGTTACT TCAGTCAAAG 
GAAGAAGAAA ATGAAATTAG CAGACAAGAG ACAGAAAAGT TGAAAGAGGA GCTTGCTGCA 
AACTCTGTTC TTACCCAGAA TCTCCAAGCA GACCTTCAGC GGAAGGAGGA AGATTGTGCT 
GAGCTAAAGG AGAAGTTCAC TGATGCCAAA AAGCAGATCG AGCAGGTACA GAGGGAGGTA 
TCTGTAATGC GTGATGAAGA GAAGTCGTTG AGGACTAAAA TTAATGAACT GGAGAAAAAG 
AAAAACCAAT ATTCTCAGGA AATAGATATG AAACAACGAA CCATTCAGCA ACTTAAGGAG 
CAGTTAAGTA ATCAGAAAAT GGAAGAAGTT GTACAACAGT ATGAGAAAGT GTGCAAAGAT 
CTCAGCGTTA AGGAGAAGCT GATTGAAGCT ATGCGACTGA CACTAGTGGA GCAGGAGCAA 
ACTCAGGCAG AGCAGGACAG GATGCTTGAG GCTAAGTCAC AGGAAGCTGA CTGGCTAGCC 
GGAGAATTGG ATACATGGAA AGACAAATTC AAAGATCTGG AGACTAGAAG TAATCAAAAG 
GTAACTACAG AGGCCATGGA GGACAGCGAC GTGCTTAGTG AGAAGTTCAG GAAGCTTCAG 
GACGAGCTGC AGGAATCTGA AGAGAAACAT AAAGCTGATA GAAAGAAGTG GTTAGAAGAA 
AAAGCGGTTC TTACAACTCA AGCAAAAGAA GCAGAGACTC TACGAAACAG GGAGATGAAA 
AAATATGCTG AGGACAGGGA GCGTTGTCTG AAGCTGCAGA ATGAAGTGGA AACACTGACA 
GCACAGCTGG CGGAGAAAAC TGGTGAGCTT CAGAAGTGGA GAGAAGAGCG GGACCAGCTG 
GTCACAGCTG TGGAGACACA GATGCAGGCT CTGCTCTCCA GCAGCAAGCA TAAGGACGAA 
GAGATCCAGC AACTGAGAAA GGCCGTGGCA AAGAGCACGG GGACAGAAAA CCAGACCATG 
AATCTCAAGC CTGAATGTAA TGATTCAGTC GATCTTGGTG GAGTTGAAAC TGAACTTCAG 
TCAACAAGTT TTGAAATTTC TAGGAATACA GCAGAGGATG GATCTGTAGT TCTTGACTCT 
TGTGAAGTGT CAACAGAAAA TGTACAAAGT ACTCGATTTC CAAAGCCTGA ACTGGAGATT 
CAGTTTACAC CTCTGCAGCC AAACAAAGTG GCAGTGAAGC ACCCTGGTTG TGCCACACCA 
GTCACAATTA AGATCCCCAA GGCACGGAAG AGGAAGAGTG GCGAGGTGGA GGAGGACTTG 
GTGAAATGTG AAAATAAGAA GAATTCCACA CCCAGAAGTA ATGTGAAGTT TCCCGTTTCA 
GAGCATAGAA ATTCCTCTTT CAAAAAGGAA CAAAAGGTTT TCTTATGTCC GTCATCTAAG 
AAGACATACT CCTTACGGAG CCAAGCATCT ACGGTTGGTG CGAATGTAGC CTCTAAAAAA 
AAAGAGGGAA CACTACAGAA ATTTGGAGAC TTCTTACAAC ATTCTCCAAC AATCCTTCAA 
TCAAAAGCAA AAAAGATAAT TGAAACAATG AGTTCCCCAA AGTTATCAAC TGTAGAAGTA 
AGTAAAGAAA ATGTATCCCA GCCTAAAAAA GCCAAACGGA AATTGTACAG AAATGAAATT 
TCATCTCCCA TCAACATATC TGGCCAAGTG ATTTTAATGG AACAGAAAGC AAAAGAAACT 
GATCATCAGA TTCTCAAACG ACGGCTTCGA ACACGAACAG CCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif20b ( XM_006231284.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231346.1 Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X5, mRNA.

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CloneID	ORa24037
Clone ID Related Accession (Same CDS sequence)	XM_006231284.2
Accession Version	XM_006231284.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF20B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006231284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTC ATTTGAATCA AGACGGAGTG CCTAGACCAT CTTATGTTTT TAGTGCTGAC 
CCAATTGCAA GGCCTTCGGA AATCAATTTT GATGGCGTTA AGCTTGATCT CTCTCATGAG 
TTTTCCTTAG TTGCCGCAAA CCCTGGGGCA AACAGTTTGG AATCTAAAAA TTACCTCCAG 
GTTTGTCTTC GAATAAGACC ATTTACACAG TCGGAAAAAG GACACGAGGC TGAGGGCTGT 
GTGCAAGTTC TGGATTCACA GACTGTTCTG CTGAAAGACC CTCAAAGCAT CCTTGGCCAC 
TTAAGTGAGA AGAGCTCCGG ACAGATGGCA CAGAAATTCA GCTTTTCTAG GGTTTTTGGA 
CCCGAAACTT CACAGAAAGA GTTCTTTCAG GGTTGCATTA TGCAGCCAGT AAAAGACCTC 
TTAAAAGGGC ACAGTCGACT GATCTTCACA TATGGGCTGA CCAATTCTGG AAAAACATAT 
ACATTTCAAG GCACAGAAGA AAACATTGGC ATTCTGCCTA GAACACTGAA TGTATTGTTT 
GATAGTCTTC AGGAAAGACT CTATACAAAG ATGAGCTTCA AACCACATAG ATGTAGAGAA 
TACCTACAGT TGTCATCTGA TCAAGAGAAA GAGGAAAGTG CTAACAAAAA TACATTGCTG 
CGACAAATTA AAGAGGTTAC CATACATAAT GACAGTTATG ACATTCTTTG TGGACGCTTA 
ACAAACTCTT TGACTATACC AGAATTTGAA GAAACCATGA ACAATTGTGA ACAATCTAGT 
CTCAATGTGG ATAATATAAA ATATTCAGTG TGGGTTTCTT TCTTTGAGAT TTACAATGAA 
AGTATTTATG ATTTGTTTGT TCCTGTGTCC TCTAAGTTGC AAAAGAGAAA AATGCTACGC 
CTTTCCCAAG ATGTAAAGGG CTATTCTTTT ATAAAAGATC TGCAGTGGAT CCAGGTATCT 
GATTCTAAAG AAGCATATAG ACTCTTAAAA CTAGGAGTGA AGCATCAGAG TGTTGCCTTC 
ACAAAACTGA ACAATGCTTC TAGCAGAAGT CACAGCATAT TCACTATTAG AATATTACAG 
ATAGAAGACT CGGAAATACC TCGTGTAACA CGAGTTAGTG AATTGTCGCT ATGTGATCTC 
GCTGGTTCAG AACGAAGCAT GAAGACGCAA AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG 
AATATCAACA CTTCTTTACT GACTCTGGGA AAGTGCATCA GTGTTCTGAA GAACAGTGAC 
AAGTCCAAGA TTCAACAACA TGTGCCTTTT CGAGAAAGTA AACTGACCCA CTATTTCCAA 
AGCTTTTTTA CTGGAAAGGG GAAAATATGT ATGATCATCA ATATTAGTCA GTGTTGTTCT 
GCCTATGATG AGACACTCAA TGTACTGAAG TTCTCAACTG TTGCACAAAA GGTCTATGTT 
CCAGATACTT TAAGTTCTTC TCAAGAGAAA TCGTTTGGGT CTACCAAATC TTTACAAGAT 
GTATCCCTAG GCAGTAATTT GGATAATAAA ATACTAAATG TAAAAAGAAA AACTGTTTCA 
TGGGAAAATA GTCTGGAAGA TGTGGTTGAA AATGAAGATT TGGTCGAGGA CTTAGAAGAA 
AATGAAGAAA CACAAAACAT GGAAACTGAA CTTACAGATG AAGATAGTGA TAAGCCGTTA 
GAGGAAGGCG GAGTCTGCGC AGGTCACGGG AAGAACAAGA AACTGTTGGA TTTAATAGAA 
AACTTGAAAA AAAGACTAAT AAATGAAAAA AAAGAAAAGT TAACATTGGA ATTTAAAATC 
CGAGAAGAAG TGACACAGGA ATTCACTCAG TATTGGAGCC AAAGGGAAGC TGATTTTAAG 
GAAACACTTC TTCATGAACG GGAGATATTA GAAGAAAATG CTGAACGTCG CTTAGCAATT 
TTCAAAGATT TGGTTGGAAA ACCTGGTGAG AGTCAAGATG AACCAGCAAG CAGATTTTGT 
ACCATGGAAC TTGAAACTGA AGAGGCTATT GCTTGTTTGC AGCTAAAGTA TAATCAAGTT 
AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA TTAATTAAAG CCCAAGAAGA GCTAAAAAAT 
AAAGAGAGTG ACTCTCTGGT TCAGGCACTC AAGACATCAA GTAAGGTTGA TACATCTTTA 
ATCAGCAATA AGTCCACTGG TAATGAAACA ACTGAAATGC CCAAGAAGAG TAGAACCCAG 
ACACATTCAG AAAGGAAAAG ATTAAATGAA GATGGACTTC AGCTTGGTGA ACCACCAGCA 
AAGAAAGGGC TTATACTTAT TAGTCCACCC ATCACTGAAG ACCAAGACAA AAGGGAAGAA 
ATGCAACAGT CTGTTTCAGA AGGTGCAGAA GAAGACAGTA GAGTTTTGCA GGAAAAGAAT 
GAAGAGCTAA AAAGACTTTT AACTATTGGT GAGAATGAAC TCAGAAATGC AAAGGAGGAA 
AAGGCAGAAT TAAATAAACA GGTTGTGAGT TTGCAGCAGC AACTTTGTTT CTTTGAAGAA 
AAGAACTCAT CCCTGAGGGC AGAGGTAGAG CAAATCCAGG CCAGTTATGA CCTTGCAGCT 
GCTGAGCTGC ATACTCAGAG AGCTGTGAAC CAGGAGCAGA AGGACAGGAT CCTGCAATTG 
TCAGGGAAGA TGGAAACTGC GGCAAGACGT ATTGAAAGCA ATGTTTCACA GATAAAGCAA 
ATGCAAACAA AAATAGATGA ACTGCGATCT CTTGATTCAC CTTCTCATAT TTCAAAAATA 
GATTTACTCA ATCTCCAGGA TCTATCAAGT GGTGCTAATT TGCTGAACAC ATCACAACAG 
CTTCCAGGTA GTGACCTACC GAGCACATGG GTTAAAGAGT TTCATACTCA GGAGCTCAGC 
AGGGAAAGTT CCTTCCACTC TAGCATTGAA GCCATCTGGG AAGAATGTAA AGAGATTGTG 
AAGGCTTCCT CCAAAAAGTC TCATCAGATT CAGGGACTAG AAGAACTAAT TGAAAAATTA 
CAGGTGGAAG TAAAAAACTG CAGGGATGAG AACAGTGAGC TTCGAGCAAA GGAGAGTGAG 
GATAAGAATC GAGACCAGCA GCTGAAGGAG AAAGAAAGTC TAATCCAGCA GCTTAGAGAA 
GAACTTCAGG AAACCACTGT CAGTCTCCGT GTCCAAGTAC AGCTAGTAGC GGAAAGAGAG 
CAAGCTCTTT CAGAGCTTTC CCGGGATGTT ACCTGCTATA AGGCAAAAGT AAAGGACCTT 
GAAGTGATGG TAGAGACCCA GAAAGAGGAA TGTAAGCGCT TAGCTGAGTT AGAGCAGAGC 
ATTTTAGAGA AGGAATCTGC CATCCTAAAG CTGGAAGCGA GTCTAAAGGA ATTGGAAGCA 
AAGCACCAGG ATCACATCAG AAGTACCACA CATTTAAATG CAGAGGAAGT CAAGTTCAGA 
GAAGAAATTA CACAGTTGGC GAATAATTTG CATGATACAA AACAGTTACT TCAGTCAAAG 
GAAGAAGAAA ATGAAATTAG CAGACAAGAG ACAGAAAAGT TGAAAGAGGA GCTTGCTGCA 
AACTCTGTTC TTACCCAGAA TCTCCAAGCA GACCTTCAGC GGAAGGAGGA AGATTGTGCT 
GAGCTAAAGG AGAAGTTCAC TGATGCCAAA AAGCAGATCG AGCAGGTACA GAGGGAGGTA 
TCTGTAATGC GTGATGAAGA GAAGTCGTTG AGGACTAAAA TTAATGAACT GGAGAAAAAG 
AAAAACCAAT ATTCTCAGGA AATAGATATG AAACAACGAA CCATTCAGCA ACTTAAGGAG 
CAGTTAAGTA ATCAGAAAAT GGAAGAAGTT GTACAACAGT ATGAGAAAGT GTGCAAAGAT 
CTCAGCGTTA AGGAGAAGCT GATTGAAGCT ATGCGACTGA CACTAGTGGA GCAGGAGCAA 
ACTCAGGCAG AGCAGGACAG GATGCTTGAG GCTAAGTCAC AGGAAGCTGA CTGGCTAGCC 
GGAGAATTGG ATACATGGAA AGACAAATTC AAAGATCTGG AGACTAGAAG TAATCAAAAG 
GTAACTACAG AGGCCATGGA GGACAGCGAC GTGCTTAGTG AGAAGTTCAG GAAGCTTCAG 
GACGAGCTGC AGGAATCTGA AGAGAAACAT AAAGCTGATA GAAAGAAGTG GTTAGAAGAA 
AAAGCGGTTC TTACAACTCA AGCAAAAGAA GCAGAGACTC TACGAAACAG GGAGATGAAA 
AAATATGCTG AGGACAGGGA GCGTTGTCTG AAGCTGCAGA ATGAAGTGGA AACACTGACA 
GCACAGCTGG CGGAGAAAAC TGGTGAGCTT CAGAAGTGGA GAGAAGAGCG GGACCAGCTG 
GTCACAGCTG TGGAGACACA GATGCAGGCT CTGCTCTCCA GCAGCAAGCA TAAGGACGAA 
GAGATCCAGC AACTGAGAAA GGCCGTGGCA AAGAGCACGG GGACAGTGAG TGGACGCGTA 
CTCGAAAACC AGACCATGAA TCTCAAGCCT GAATGTAATG ATTCAGTCGA TCTTGGTGGA 
GTTGAAACTG AACTTCAGTC AACAAGTTTT GAAATTTCTA GGAATACAGC AGAGGATGGA 
TCTGTAGTTC TTGACTCTTG TGAAGTGTCA ACAGAAAATG TACAAAGTAC TCGATTTCCA 
AAGCCTGAAC TGGAGATTCA GTTTACACCT CTGCAGCCAA ACAAAGTGGC AGTGAAGCAC 
CCTGGTTGTG CCACACCAGT CACAATTAAG ATCCCCAAGG CACGGAAGAG GAAGAGTGGC 
GAGGTGGAGG AGGACTTGGT GAAATGTGAA AATAAGAAGA ATTCCACACC CAGAAGTAAT 
GTGAAGTTTC CCGTTTCAGA GCATAGAAAT TCCTCTTTCA AAAAGGAACA AAAGGTTTTC 
TTATGTCCGT CATCTAAGAA GACATACTCC TTACGGAGCC AAGCATCTAC GGTTGGTGCG 
AATGTAGCCT CTAAAAAAAA AGAGGGAACA CTACAGAAAT TTGGAGACTT CTTACAACAT 
TCTCCAACAA TCCTTCAATC AAAAGCAAAA AAGATAATTG AAACAATGAG TTCCCCAAAG 
TTATCAACTG TAGAAGTAAG TAAAGAAAAT GTATCCCAGC CTAAAAAAGC CAAACGGAAA 
TTGTACAGAA ATGAAATTTC ATCTCCCATC AACATATCTG GCCAAGTGAT TTTAATGGAA 
CAGAAAGCAA AAGAAACTGA TCATCAGATT CTCAAACGAC GGCTTCGAAC ACGAACAGCC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231346.1
CDS	120..5345
Translation	MESHLNQDGVPRPSYVFSADPIARPSEINFDGVKLDLSHEFSLVAANPGANSLESKNYLQVCLRIRPFTQSEKGHEAEGCVQVLDSQTVLLKDPQSILGHLSEKSSGQMAQKFSFSRVFGPETSQKEFFQGCIMQPVKDLLKGHSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLFDSLQERLYTKMSFKPHRCREYLQLSSDQEKEESANKNTLLRQIKEVTIHNDSYDILCGRLTNSLTIPEFEETMNNCEQSSLNVDNIKYSVWVSFFEIYNESIYDLFVPVSSKLQKRKMLRLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGVKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPRVTRVSELSLCDLAGSERSMKTQSEGERLREAGNINTSLLTLGKCISVLKNSDKSKIQQHVPFRESKLTHYFQSFFTGKGKICMIINISQCCSAYDETLNVLKFSTVAQKVYVPDTLSSSQEKSFGSTKSLQDVSLGSNLDNKILNVKRKTVSWENSLEDVVENEDLVEDLEENEETQNMETELTDEDSDKPLEEGGVCAGHGKNKKLLDLIENLKKRLINEKKEKLTLEFKIREEVTQEFTQYWSQREADFKETLLHEREILEENAERRLAIFKDLVGKPGESQDEPASRFCTMELETEEAIACLQLKYNQVKAELAETKEELIKAQEELKNKESDSLVQALKTSSKVDTSLISNKSTGNETTEMPKKSRTQTHSERKRLNEDGLQLGEPPAKKGLILISPPITEDQDKREEMQQSVSEGAEEDSRVLQEKNEELKRLLTIGENELRNAKEEKAELNKQVVSLQQQLCFFEEKNSSLRAEVEQIQASYDLAAAELHTQRAVNQEQKDRILQLSGKMETAARRIESNVSQIKQMQTKIDELRSLDSPSHISKIDLLNLQDLSSGANLLNTSQQLPGSDLPSTWVKEFHTQELSRESSFHSSIEAIWEECKEIVKASSKKSHQIQGLEELIEKLQVEVKNCRDENSELRAKESEDKNRDQQLKEKESLIQQLREELQETTVSLRVQVQLVAEREQALSELSRDVTCYKAKVKDLEVMVETQKEECKRLAELEQSILEKESAILKLEASLKELEAKHQDHIRSTTHLNAEEVKFREEITQLANNLHDTKQLLQSKEEENEISRQETEKLKEELAANSVLTQNLQADLQRKEEDCAELKEKFTDAKKQIEQVQREVSVMRDEEKSLRTKINELEKKKNQYSQEIDMKQRTIQQLKEQLSNQKMEEVVQQYEKVCKDLSVKEKLIEAMRLTLVEQEQTQAEQDRMLEAKSQEADWLAGELDTWKDKFKDLETRSNQKVTTEAMEDSDVLSEKFRKLQDELQESEEKHKADRKKWLEEKAVLTTQAKEAETLRNREMKKYAEDRERCLKLQNEVETLTAQLAEKTGELQKWREERDQLVTAVETQMQALLSSSKHKDEEIQQLRKAVAKSTGTVSGRVLENQTMNLKPECNDSVDLGGVETELQSTSFEISRNTAEDGSVVLDSCEVSTENVQSTRFPKPELEIQFTPLQPNKVAVKHPGCATPVTIKIPKARKRKSGEVEEDLVKCENKKNSTPRSNVKFPVSEHRNSSFKKEQKVFLCPSSKKTYSLRSQASTVGANVASKKKEGTLQKFGDFLQHSPTILQSKAKKIIETMSSPKLSTVEVSKENVSQPKKAKRKLYRNEISSPINISGQVILMEQKAKETDHQILKRRLRTRTAK

Target ORF information:

RefSeq Version	XM_006231284.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231284.2

ORF Insert Sequence:

ATGGAATCTC ATTTGAATCA AGACGGAGTG CCTAGACCAT CTTATGTTTT TAGTGCTGAC 
CCAATTGCAA GGCCTTCGGA AATCAATTTT GATGGCGTTA AGCTTGATCT CTCTCATGAG 
TTTTCCTTAG TTGCCGCAAA CCCTGGGGCA AACAGTTTGG AATCTAAAAA TTACCTCCAG 
GTTTGTCTTC GAATAAGACC ATTTACACAG TCGGAAAAAG GACACGAGGC TGAGGGCTGT 
GTGCAAGTTC TGGATTCACA GACTGTTCTG CTGAAAGACC CTCAAAGCAT CCTTGGCCAC 
TTAAGTGAGA AGAGCTCCGG ACAGATGGCA CAGAAATTCA GCTTTTCTAG GGTTTTTGGA 
CCCGAAACTT CACAGAAAGA GTTCTTTCAG GGTTGCATTA TGCAGCCAGT AAAAGACCTC 
TTAAAAGGGC ACAGTCGACT GATCTTCACA TATGGGCTGA CCAATTCTGG AAAAACATAT 
ACATTTCAAG GCACAGAAGA AAACATTGGC ATTCTGCCTA GAACACTGAA TGTATTGTTT 
GATAGTCTTC AGGAAAGACT CTATACAAAG ATGAGCTTCA AACCACATAG ATGTAGAGAA 
TACCTACAGT TGTCATCTGA TCAAGAGAAA GAGGAAAGTG CTAACAAAAA TACATTGCTG 
CGACAAATTA AAGAGGTTAC CATACATAAT GACAGTTATG ACATTCTTTG TGGACGCTTA 
ACAAACTCTT TGACTATACC AGAATTTGAA GAAACCATGA ACAATTGTGA ACAATCTAGT 
CTCAATGTGG ATAATATAAA ATATTCAGTG TGGGTTTCTT TCTTTGAGAT TTACAATGAA 
AGTATTTATG ATTTGTTTGT TCCTGTGTCC TCTAAGTTGC AAAAGAGAAA AATGCTACGC 
CTTTCCCAAG ATGTAAAGGG CTATTCTTTT ATAAAAGATC TGCAGTGGAT CCAGGTATCT 
GATTCTAAAG AAGCATATAG ACTCTTAAAA CTAGGAGTGA AGCATCAGAG TGTTGCCTTC 
ACAAAACTGA ACAATGCTTC TAGCAGAAGT CACAGCATAT TCACTATTAG AATATTACAG 
ATAGAAGACT CGGAAATACC TCGTGTAACA CGAGTTAGTG AATTGTCGCT ATGTGATCTC 
GCTGGTTCAG AACGAAGCAT GAAGACGCAA AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG 
AATATCAACA CTTCTTTACT GACTCTGGGA AAGTGCATCA GTGTTCTGAA GAACAGTGAC 
AAGTCCAAGA TTCAACAACA TGTGCCTTTT CGAGAAAGTA AACTGACCCA CTATTTCCAA 
AGCTTTTTTA CTGGAAAGGG GAAAATATGT ATGATCATCA ATATTAGTCA GTGTTGTTCT 
GCCTATGATG AGACACTCAA TGTACTGAAG TTCTCAACTG TTGCACAAAA GGTCTATGTT 
CCAGATACTT TAAGTTCTTC TCAAGAGAAA TCGTTTGGGT CTACCAAATC TTTACAAGAT 
GTATCCCTAG GCAGTAATTT GGATAATAAA ATACTAAATG TAAAAAGAAA AACTGTTTCA 
TGGGAAAATA GTCTGGAAGA TGTGGTTGAA AATGAAGATT TGGTCGAGGA CTTAGAAGAA 
AATGAAGAAA CACAAAACAT GGAAACTGAA CTTACAGATG AAGATAGTGA TAAGCCGTTA 
GAGGAAGGCG GAGTCTGCGC AGGTCACGGG AAGAACAAGA AACTGTTGGA TTTAATAGAA 
AACTTGAAAA AAAGACTAAT AAATGAAAAA AAAGAAAAGT TAACATTGGA ATTTAAAATC 
CGAGAAGAAG TGACACAGGA ATTCACTCAG TATTGGAGCC AAAGGGAAGC TGATTTTAAG 
GAAACACTTC TTCATGAACG GGAGATATTA GAAGAAAATG CTGAACGTCG CTTAGCAATT 
TTCAAAGATT TGGTTGGAAA ACCTGGTGAG AGTCAAGATG AACCAGCAAG CAGATTTTGT 
ACCATGGAAC TTGAAACTGA AGAGGCTATT GCTTGTTTGC AGCTAAAGTA TAATCAAGTT 
AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA TTAATTAAAG CCCAAGAAGA GCTAAAAAAT 
AAAGAGAGTG ACTCTCTGGT TCAGGCACTC AAGACATCAA GTAAGGTTGA TACATCTTTA 
ATCAGCAATA AGTCCACTGG TAATGAAACA ACTGAAATGC CCAAGAAGAG TAGAACCCAG 
ACACATTCAG AAAGGAAAAG ATTAAATGAA GATGGACTTC AGCTTGGTGA ACCACCAGCA 
AAGAAAGGGC TTATACTTAT TAGTCCACCC ATCACTGAAG ACCAAGACAA AAGGGAAGAA 
ATGCAACAGT CTGTTTCAGA AGGTGCAGAA GAAGACAGTA GAGTTTTGCA GGAAAAGAAT 
GAAGAGCTAA AAAGACTTTT AACTATTGGT GAGAATGAAC TCAGAAATGC AAAGGAGGAA 
AAGGCAGAAT TAAATAAACA GGTTGTGAGT TTGCAGCAGC AACTTTGTTT CTTTGAAGAA 
AAGAACTCAT CCCTGAGGGC AGAGGTAGAG CAAATCCAGG CCAGTTATGA CCTTGCAGCT 
GCTGAGCTGC ATACTCAGAG AGCTGTGAAC CAGGAGCAGA AGGACAGGAT CCTGCAATTG 
TCAGGGAAGA TGGAAACTGC GGCAAGACGT ATTGAAAGCA ATGTTTCACA GATAAAGCAA 
ATGCAAACAA AAATAGATGA ACTGCGATCT CTTGATTCAC CTTCTCATAT TTCAAAAATA 
GATTTACTCA ATCTCCAGGA TCTATCAAGT GGTGCTAATT TGCTGAACAC ATCACAACAG 
CTTCCAGGTA GTGACCTACC GAGCACATGG GTTAAAGAGT TTCATACTCA GGAGCTCAGC 
AGGGAAAGTT CCTTCCACTC TAGCATTGAA GCCATCTGGG AAGAATGTAA AGAGATTGTG 
AAGGCTTCCT CCAAAAAGTC TCATCAGATT CAGGGACTAG AAGAACTAAT TGAAAAATTA 
CAGGTGGAAG TAAAAAACTG CAGGGATGAG AACAGTGAGC TTCGAGCAAA GGAGAGTGAG 
GATAAGAATC GAGACCAGCA GCTGAAGGAG AAAGAAAGTC TAATCCAGCA GCTTAGAGAA 
GAACTTCAGG AAACCACTGT CAGTCTCCGT GTCCAAGTAC AGCTAGTAGC GGAAAGAGAG 
CAAGCTCTTT CAGAGCTTTC CCGGGATGTT ACCTGCTATA AGGCAAAAGT AAAGGACCTT 
GAAGTGATGG TAGAGACCCA GAAAGAGGAA TGTAAGCGCT TAGCTGAGTT AGAGCAGAGC 
ATTTTAGAGA AGGAATCTGC CATCCTAAAG CTGGAAGCGA GTCTAAAGGA ATTGGAAGCA 
AAGCACCAGG ATCACATCAG AAGTACCACA CATTTAAATG CAGAGGAAGT CAAGTTCAGA 
GAAGAAATTA CACAGTTGGC GAATAATTTG CATGATACAA AACAGTTACT TCAGTCAAAG 
GAAGAAGAAA ATGAAATTAG CAGACAAGAG ACAGAAAAGT TGAAAGAGGA GCTTGCTGCA 
AACTCTGTTC TTACCCAGAA TCTCCAAGCA GACCTTCAGC GGAAGGAGGA AGATTGTGCT 
GAGCTAAAGG AGAAGTTCAC TGATGCCAAA AAGCAGATCG AGCAGGTACA GAGGGAGGTA 
TCTGTAATGC GTGATGAAGA GAAGTCGTTG AGGACTAAAA TTAATGAACT GGAGAAAAAG 
AAAAACCAAT ATTCTCAGGA AATAGATATG AAACAACGAA CCATTCAGCA ACTTAAGGAG 
CAGTTAAGTA ATCAGAAAAT GGAAGAAGTT GTACAACAGT ATGAGAAAGT GTGCAAAGAT 
CTCAGCGTTA AGGAGAAGCT GATTGAAGCT ATGCGACTGA CACTAGTGGA GCAGGAGCAA 
ACTCAGGCAG AGCAGGACAG GATGCTTGAG GCTAAGTCAC AGGAAGCTGA CTGGCTAGCC 
GGAGAATTGG ATACATGGAA AGACAAATTC AAAGATCTGG AGACTAGAAG TAATCAAAAG 
GTAACTACAG AGGCCATGGA GGACAGCGAC GTGCTTAGTG AGAAGTTCAG GAAGCTTCAG 
GACGAGCTGC AGGAATCTGA AGAGAAACAT AAAGCTGATA GAAAGAAGTG GTTAGAAGAA 
AAAGCGGTTC TTACAACTCA AGCAAAAGAA GCAGAGACTC TACGAAACAG GGAGATGAAA 
AAATATGCTG AGGACAGGGA GCGTTGTCTG AAGCTGCAGA ATGAAGTGGA AACACTGACA 
GCACAGCTGG CGGAGAAAAC TGGTGAGCTT CAGAAGTGGA GAGAAGAGCG GGACCAGCTG 
GTCACAGCTG TGGAGACACA GATGCAGGCT CTGCTCTCCA GCAGCAAGCA TAAGGACGAA 
GAGATCCAGC AACTGAGAAA GGCCGTGGCA AAGAGCACGG GGACAGTGAG TGGACGCGTA 
CTCGAAAACC AGACCATGAA TCTCAAGCCT GAATGTAATG ATTCAGTCGA TCTTGGTGGA 
GTTGAAACTG AACTTCAGTC AACAAGTTTT GAAATTTCTA GGAATACAGC AGAGGATGGA 
TCTGTAGTTC TTGACTCTTG TGAAGTGTCA ACAGAAAATG TACAAAGTAC TCGATTTCCA 
AAGCCTGAAC TGGAGATTCA GTTTACACCT CTGCAGCCAA ACAAAGTGGC AGTGAAGCAC 
CCTGGTTGTG CCACACCAGT CACAATTAAG ATCCCCAAGG CACGGAAGAG GAAGAGTGGC 
GAGGTGGAGG AGGACTTGGT GAAATGTGAA AATAAGAAGA ATTCCACACC CAGAAGTAAT 
GTGAAGTTTC CCGTTTCAGA GCATAGAAAT TCCTCTTTCA AAAAGGAACA AAAGGTTTTC 
TTATGTCCGT CATCTAAGAA GACATACTCC TTACGGAGCC AAGCATCTAC GGTTGGTGCG 
AATGTAGCCT CTAAAAAAAA AGAGGGAACA CTACAGAAAT TTGGAGACTT CTTACAACAT 
TCTCCAACAA TCCTTCAATC AAAAGCAAAA AAGATAATTG AAACAATGAG TTCCCCAAAG 
TTATCAACTG TAGAAGTAAG TAAAGAAAAT GTATCCCAGC CTAAAAAAGC CAAACGGAAA 
TTGTACAGAA ATGAAATTTC ATCTCCCATC AACATATCTG GCCAAGTGAT TTTAATGGAA 
CAGAAAGCAA AAGAAACTGA TCATCAGATT CTCAAACGAC GGCTTCGAAC ACGAACAGCC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif20b ( XM_006231285.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231347.1 Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X6, mRNA.

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CloneID	ORa24038
Clone ID Related Accession (Same CDS sequence)	XM_006231285.2
Accession Version	XM_006231285.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF20B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006231285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTC ATTTGAATCA AGACGGAGTG CCTAGACCAT CTTATGTTTT TAGTGCTGAC 
CCAATTGCAA GGCCTTCGGA AATCAATTTT GATGGCGTTA AGCTTGATCT CTCTCATGAG 
TTTTCCTTAG TTGCCGCAAA CCCTGGGGCA AACAGTTTGG AATCTAAAAA TTACCTCCAG 
GTTTGTCTTC GAATAAGACC ATTTACACAG TCGGAAAAAG GACACGAGGC TGAGGGCTGT 
GTGCAAGTTC TGGATTCACA GACTGTTCTG CTGAAAGACC CTCAAAGCAT CCTTGGCCAC 
TTAAGTGAGA AGAGCTCCGG ACAGATGGCA CAGAAATTCA GCTTTTCTAG GGTTTTTGGA 
CCCGAAACTT CACAGAAAGA GTTCTTTCAG GGTTGCATTA TGCAGCCAGT AAAAGACCTC 
TTAAAAGGGC ACAGTCGACT GATCTTCACA TATGGGCTGA CCAATTCTGG AAAAACATAT 
ACATTTCAAG GCACAGAAGA AAACATTGGC ATTCTGCCTA GAACACTGAA TGTATTGTTT 
GATAGTCTTC AGGAAAGACT CTATACAAAG ATGAGCTTCA AACCACATAG ATGTAGAGAA 
TACCTACAGT TGTCATCTGA TCAAGAGAAA GAGGAAAGTG CTAACAAAAA TACATTGCTG 
CGACAAATTA AAGAGGTTAC CATACATAAT GACAGTTATG ACATTCTTTG TGGACGCTTA 
ACAAACTCTT TGACTATACC AGAATTTGAA GAAACCATGA ACAATTGTGA ACAATCTAGT 
CTCAATGTGG ATAATATAAA ATATTCAGTG TGGGTTTCTT TCTTTGAGAT TTACAATGAA 
AGTATTTATG ATTTGTTTGT TCCTGTGTCC TCTAAGTTGC AAAAGAGAAA AATGCTACGC 
CTTTCCCAAG ATGTAAAGGG CTATTCTTTT ATAAAAGATC TGCAGTGGAT CCAGGTATCT 
GATTCTAAAG AAGCATATAG ACTCTTAAAA CTAGGAGTGA AGCATCAGAG TGTTGCCTTC 
ACAAAACTGA ACAATGCTTC TAGCAGAAGT CACAGCATAT TCACTATTAG AATATTACAG 
ATAGAAGACT CGGAAATACC TCGTGTAACA CGAGTTAGTG AATTGTCGCT ATGTGATCTC 
GCTGGTTCAG AACGAAGCAT GAAGACGCAA AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG 
AATATCAACA CTTCTTTACT GACTCTGGGA AAGTGCATCA GTGTTCTGAA GAACAGTGAC 
AAGTCCAAGA TTCAACAACA TGTGCCTTTT CGAGAAAGTA AACTGACCCA CTATTTCCAA 
AGCTTTTTTA CTGGAAAGGG GAAAATATGT ATGATCATCA ATATTAGTCA GTGTTGTTCT 
GCCTATGATG AGACACTCAA TGTACTGAAG TTCTCAACTG TTGCACAAAA GGTCTATGTT 
CCAGATACTT TAAGTTCTTC TCAAGAGAAA TCGTTTGGGT CTACCAAATC TTTACAAGAT 
GTATCCCTAG GCAGTAATTT GGATAATAAA ATACTAAATG TAAAAAGAAA AACTGTTTCA 
TGGGAAAATA GTCTGGAAGA TGTGGTTGAA AATGAAGATT TGGTCGAGGA CTTAGAAGAA 
AATGAAGAAA CACAAAACAT GGAAACTGAA CTTACAGATG AAGATAGTGA TAAGCCGTTA 
GAGGAAGGCG GAGTCTGCGC AGGTCACGGG AAGAACAAGA AACTGTTGGA TTTAATAGAA 
AACTTGAAAA AAAGACTAAT AAATGAAAAA AAAGAAAAGT TAACATTGGA ATTTAAAATC 
CGAGAAGAAG TGACACAGGA ATTCACTCAG TATTGGAGCC AAAGGGAAGC TGATTTTAAG 
GAAACACTTC TTCATGAACG GGAGATATTA GAAGAAAATG CTGAACGTCG CTTAGCAATT 
TTCAAAGATT TGGTTGGAAA ACCTGGTGAG AGTCAAGATG AACCAGCAAG CAGATTTTGT 
ACCATGGAAC TTGAAACTGA AGAGGCTATT GCTTGTTTGC AGCTAAAGTA TAATCAAGTT 
AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA TTAATTAAAG CCCAAGAAGA GCTAAAAAAT 
AAAGAGAGTG ACTCTCTGGT TCAGGCACTC AAGACATCAA GTAAGGTTGA TACATCTTTA 
ATCAGCAATA AGTCCACTGG TAATGAAACA ACTGAAATGC CCAAGAAGAG TAGAACCCAG 
ACACATTCAG AAAGGAAAAG ATTAAATGAA GATGGACTTC AGCTTGGTGA ACCACCAGCA 
AAGAAAGGGC TTATACTTAT TAGTCCACCC ATCACTGAAG ACCAAGACAA AAGGGAAGAA 
ATGCAACAGT CTGTTTCAGA AGGTGCAGAA GAAGACAGTA GAGTTTTGCA GGAAAAGAAT 
GAAGAGCTAA AAAGACTTTT AACTATTGGT GAGAATGAAC TCAGAAATGC AAAGGAGGAA 
AAGGCAGAAT TAAATAAACA GGTTGTGAGT TTGCAGCAGC AACTTTGTTT CTTTGAAGAA 
AAGAACTCAT CCCTGAGGGC AGAGGTAGAG CAAATCCAGG CCAGTTATGA CCTTGCAGCT 
GCTGAGCTGC ATACTCAGAG AGCTGTGAAC CAGGAGCAGA AGGACAGGAT CCTGCAATTG 
TCAGGGAAGA TGGAAACTGC GGCAAGACGT ATTGAAAGCA ATGTTTCACA GATAAAGCAA 
ATGCAAACAA AAATAGATGA ACTGCGATCT CTTGATTCAC CTTCTCATAT TTCAAAAATA 
GATTTACTCA ATCTCCAGGA TCTATCAAGT GGTGCTAATT TGCTGAACAC ATCACAACAG 
CTTCCAGGTA GTGACCTACC GAGCACATGG GTTAAAGAGT TTCATACTCA GGAGCTCAGC 
AGGGAAAGTT CCTTCCACTC TAGCATTGAA GCCATCTGGG AAGAATGTAA AGAGATTGTG 
AAGGCTTCCT CCAAAAAGTC TCATCAGATT CAGGGACTAG AAGAACTAAT TGAAAAATTA 
CAGGTGGAAG TAAAAAACTG CAGGGATGAG AACAGTGAGC TTCGAGCAAA GGAGAGTGAG 
GATAAGAATC GAGACCAGCA GCTGAAGGAG AAAGAAAGTC TAATCCAGCA GCTTAGAGAA 
GAACTTCAGG AAACCACTGT CAGTCTCCGT GTCCAAGTAC AGCTAGTAGC GGAAAGAGAG 
CAAGCTCTTT CAGAGCTTTC CCGGGATGTT ACCTGCTATA AGGCAAAAGT AAAGGACCTT 
GAAGTGATGG TAGAGACCCA GAAAGAGGAA TGTAAGCGCT TAGCTGAGTT AGAGCAGAGC 
ATTTTAGAGA AGGAATCTGC CATCCTAAAG CTGGAAGCGA GTCTAAAGGA ATTGGAAGCA 
AAGCACCAGG ATCACATCAG AAGTACCACA CATTTAAATG CAGAGGAAGT CAAGTTCAGA 
GAAGAAATTA CACAGTTGGC GAATAATTTG CATGATACAA AACAGTTACT TCAGTCAAAG 
GAAGAAGAAA ATGAAATTAG CAGACAAGAG ACAGAAAAGT TGAAAGAGGA GCTTGCTGCA 
AACTCTGTTC TTACCCAGAA TCTCCAAGCA GACCTTCAGC GGAAGGAGGA AGATTGTGCT 
GAGCTAAAGG AGAAGTTCAC TGATGCCAAA AAGCAGATCG AGCAGGTACA GAGGGAGGTA 
TCTGTAATGC GTGATGAAGA GAAGTCGTTG AGGACTAAAA TTAATGAACT GGAGAAAAAG 
AAAAACCAAT ATTCTCAGGA AATAGATATG AAACAACGAA CCATTCAGCA ACTTAAGGAG 
CAGTTAAGTA ATCAGAAAAT GGAAGAAGTT GTACAACAGT ATGAGAAAGT GTGCAAAGAT 
CTCAGCGTTA AGGAGAAGCT GATTGAAGCT ATGCGACTGA CACTAGTGGA GCAGGAGCAA 
ACTCAGGCAG AGCAGGACAG GATGCTTGAG GCTAAGTCAC AGGAAGCTGA CTGGCTAGCC 
GGAGAATTGG ATACATGGAA AGACAAATTC AAAGATCTGG AGACTAGAAG TAATCAAAAG 
GTAACTACAG AGGCCATGGA GGACAGCGAC GTGCTTAGTG AGAAGTTCAG GAAGCTTCAG 
GACGAGCTGC AGGAATCTGA AGAGAAACAT AAAGCTGATA GAAAGAAGTG GTTAGAAGAA 
AAAGCGGTTC TTACAACTCA AGCAAAAGAA GCAGAGACTC TACGAAACAG GGAGATGAAA 
AAATATGCTG AGGACAGGGA GCGTTGTCTG AAGCTGCAGA ATGAAGTGGA AACACTGACA 
GCACAGCTGG CGGAGAAAAC TGGTGAGCTT CAGAAGTGGA GAGAAGAGCG GGACCAGCTG 
GTCACAGCTG TGGAGACACA GATGCAGGCT CTGCTCTCCA GCAGCAAGCA TAAGGACGAA 
GAGATCCAGC AACTGAGAAA GGCCGTGGCA AAGAGCACGG GGACAGAAAA CCAGACCATG 
AATCTCAAGC CTGAATGTAA TGATTCAGTC GATCTTGGTG GAGTTGAAAC TGAACTTCAG 
TCAACAAGTT TTGAAATTTC TAGGAATACA GCAGAGGATG GATCTGTAGT TCTTGACTCT 
TGTGAAGTGT CAACAGAAAA TGTACAAAGT ACTCGATTTC CAAAGCCTGA ACTGGAGATT 
CAGTTTACAC CTCTGCAGCC AAACAAAGTG GCAGTGAAGC ACCCTGGTTG TGCCACACCA 
GTCACAATTA AGATCCCCAA GGCACGGAAG AGGAAGAGTG GCGAGGTGGA GGAGGACTTG 
GTGAAATGTG AAAATAAGAA GAATTCCACA CCCAGAAGTA ATGTGAAGTT TCCCGTTTCA 
GAGCATAGAA ATTCCTCTTT CAAAAAGGAA CAAAAGGTTT TCTTATGTCC GTCATCTAAG 
AAGACATACT CCTTACGGAG CCAAGCATCT ACGGTTGGTG CGAATGTAGC CTCTAAAAAA 
AAAGAGGGAA CACTACAGAA ATTTGGAGAC TTCTTACAAC ATTCTCCAAC AATCCTTCAA 
TCAAAAGCAA AAAAGATAAT TGAAACAATG AGTTCCCCAA AGTTATCAAC TGTAGAAGTA 
AGTAAAGAAA ATGTATCCCA GCCTAAAAAA GCCAAACGGA AATTGTACAG AAATGAAATT 
TCATCTCCCA TCAACATATC TGGCCAAGTG ATTTTAATGG AACAGAAAGC AAAAGAAACT 
GATCATCAGA TTCTCAAACG ACGGCTTCGA ACACGAACAG CCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231347.1
CDS	120..5327
Translation	MESHLNQDGVPRPSYVFSADPIARPSEINFDGVKLDLSHEFSLVAANPGANSLESKNYLQVCLRIRPFTQSEKGHEAEGCVQVLDSQTVLLKDPQSILGHLSEKSSGQMAQKFSFSRVFGPETSQKEFFQGCIMQPVKDLLKGHSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLFDSLQERLYTKMSFKPHRCREYLQLSSDQEKEESANKNTLLRQIKEVTIHNDSYDILCGRLTNSLTIPEFEETMNNCEQSSLNVDNIKYSVWVSFFEIYNESIYDLFVPVSSKLQKRKMLRLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGVKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPRVTRVSELSLCDLAGSERSMKTQSEGERLREAGNINTSLLTLGKCISVLKNSDKSKIQQHVPFRESKLTHYFQSFFTGKGKICMIINISQCCSAYDETLNVLKFSTVAQKVYVPDTLSSSQEKSFGSTKSLQDVSLGSNLDNKILNVKRKTVSWENSLEDVVENEDLVEDLEENEETQNMETELTDEDSDKPLEEGGVCAGHGKNKKLLDLIENLKKRLINEKKEKLTLEFKIREEVTQEFTQYWSQREADFKETLLHEREILEENAERRLAIFKDLVGKPGESQDEPASRFCTMELETEEAIACLQLKYNQVKAELAETKEELIKAQEELKNKESDSLVQALKTSSKVDTSLISNKSTGNETTEMPKKSRTQTHSERKRLNEDGLQLGEPPAKKGLILISPPITEDQDKREEMQQSVSEGAEEDSRVLQEKNEELKRLLTIGENELRNAKEEKAELNKQVVSLQQQLCFFEEKNSSLRAEVEQIQASYDLAAAELHTQRAVNQEQKDRILQLSGKMETAARRIESNVSQIKQMQTKIDELRSLDSPSHISKIDLLNLQDLSSGANLLNTSQQLPGSDLPSTWVKEFHTQELSRESSFHSSIEAIWEECKEIVKASSKKSHQIQGLEELIEKLQVEVKNCRDENSELRAKESEDKNRDQQLKEKESLIQQLREELQETTVSLRVQVQLVAEREQALSELSRDVTCYKAKVKDLEVMVETQKEECKRLAELEQSILEKESAILKLEASLKELEAKHQDHIRSTTHLNAEEVKFREEITQLANNLHDTKQLLQSKEEENEISRQETEKLKEELAANSVLTQNLQADLQRKEEDCAELKEKFTDAKKQIEQVQREVSVMRDEEKSLRTKINELEKKKNQYSQEIDMKQRTIQQLKEQLSNQKMEEVVQQYEKVCKDLSVKEKLIEAMRLTLVEQEQTQAEQDRMLEAKSQEADWLAGELDTWKDKFKDLETRSNQKVTTEAMEDSDVLSEKFRKLQDELQESEEKHKADRKKWLEEKAVLTTQAKEAETLRNREMKKYAEDRERCLKLQNEVETLTAQLAEKTGELQKWREERDQLVTAVETQMQALLSSSKHKDEEIQQLRKAVAKSTGTENQTMNLKPECNDSVDLGGVETELQSTSFEISRNTAEDGSVVLDSCEVSTENVQSTRFPKPELEIQFTPLQPNKVAVKHPGCATPVTIKIPKARKRKSGEVEEDLVKCENKKNSTPRSNVKFPVSEHRNSSFKKEQKVFLCPSSKKTYSLRSQASTVGANVASKKKEGTLQKFGDFLQHSPTILQSKAKKIIETMSSPKLSTVEVSKENVSQPKKAKRKLYRNEISSPINISGQVILMEQKAKETDHQILKRRLRTRTAK

Target ORF information:

RefSeq Version	XM_006231285.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231285.2

ORF Insert Sequence:

ATGGAATCTC ATTTGAATCA AGACGGAGTG CCTAGACCAT CTTATGTTTT TAGTGCTGAC 
CCAATTGCAA GGCCTTCGGA AATCAATTTT GATGGCGTTA AGCTTGATCT CTCTCATGAG 
TTTTCCTTAG TTGCCGCAAA CCCTGGGGCA AACAGTTTGG AATCTAAAAA TTACCTCCAG 
GTTTGTCTTC GAATAAGACC ATTTACACAG TCGGAAAAAG GACACGAGGC TGAGGGCTGT 
GTGCAAGTTC TGGATTCACA GACTGTTCTG CTGAAAGACC CTCAAAGCAT CCTTGGCCAC 
TTAAGTGAGA AGAGCTCCGG ACAGATGGCA CAGAAATTCA GCTTTTCTAG GGTTTTTGGA 
CCCGAAACTT CACAGAAAGA GTTCTTTCAG GGTTGCATTA TGCAGCCAGT AAAAGACCTC 
TTAAAAGGGC ACAGTCGACT GATCTTCACA TATGGGCTGA CCAATTCTGG AAAAACATAT 
ACATTTCAAG GCACAGAAGA AAACATTGGC ATTCTGCCTA GAACACTGAA TGTATTGTTT 
GATAGTCTTC AGGAAAGACT CTATACAAAG ATGAGCTTCA AACCACATAG ATGTAGAGAA 
TACCTACAGT TGTCATCTGA TCAAGAGAAA GAGGAAAGTG CTAACAAAAA TACATTGCTG 
CGACAAATTA AAGAGGTTAC CATACATAAT GACAGTTATG ACATTCTTTG TGGACGCTTA 
ACAAACTCTT TGACTATACC AGAATTTGAA GAAACCATGA ACAATTGTGA ACAATCTAGT 
CTCAATGTGG ATAATATAAA ATATTCAGTG TGGGTTTCTT TCTTTGAGAT TTACAATGAA 
AGTATTTATG ATTTGTTTGT TCCTGTGTCC TCTAAGTTGC AAAAGAGAAA AATGCTACGC 
CTTTCCCAAG ATGTAAAGGG CTATTCTTTT ATAAAAGATC TGCAGTGGAT CCAGGTATCT 
GATTCTAAAG AAGCATATAG ACTCTTAAAA CTAGGAGTGA AGCATCAGAG TGTTGCCTTC 
ACAAAACTGA ACAATGCTTC TAGCAGAAGT CACAGCATAT TCACTATTAG AATATTACAG 
ATAGAAGACT CGGAAATACC TCGTGTAACA CGAGTTAGTG AATTGTCGCT ATGTGATCTC 
GCTGGTTCAG AACGAAGCAT GAAGACGCAA AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG 
AATATCAACA CTTCTTTACT GACTCTGGGA AAGTGCATCA GTGTTCTGAA GAACAGTGAC 
AAGTCCAAGA TTCAACAACA TGTGCCTTTT CGAGAAAGTA AACTGACCCA CTATTTCCAA 
AGCTTTTTTA CTGGAAAGGG GAAAATATGT ATGATCATCA ATATTAGTCA GTGTTGTTCT 
GCCTATGATG AGACACTCAA TGTACTGAAG TTCTCAACTG TTGCACAAAA GGTCTATGTT 
CCAGATACTT TAAGTTCTTC TCAAGAGAAA TCGTTTGGGT CTACCAAATC TTTACAAGAT 
GTATCCCTAG GCAGTAATTT GGATAATAAA ATACTAAATG TAAAAAGAAA AACTGTTTCA 
TGGGAAAATA GTCTGGAAGA TGTGGTTGAA AATGAAGATT TGGTCGAGGA CTTAGAAGAA 
AATGAAGAAA CACAAAACAT GGAAACTGAA CTTACAGATG AAGATAGTGA TAAGCCGTTA 
GAGGAAGGCG GAGTCTGCGC AGGTCACGGG AAGAACAAGA AACTGTTGGA TTTAATAGAA 
AACTTGAAAA AAAGACTAAT AAATGAAAAA AAAGAAAAGT TAACATTGGA ATTTAAAATC 
CGAGAAGAAG TGACACAGGA ATTCACTCAG TATTGGAGCC AAAGGGAAGC TGATTTTAAG 
GAAACACTTC TTCATGAACG GGAGATATTA GAAGAAAATG CTGAACGTCG CTTAGCAATT 
TTCAAAGATT TGGTTGGAAA ACCTGGTGAG AGTCAAGATG AACCAGCAAG CAGATTTTGT 
ACCATGGAAC TTGAAACTGA AGAGGCTATT GCTTGTTTGC AGCTAAAGTA TAATCAAGTT 
AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA TTAATTAAAG CCCAAGAAGA GCTAAAAAAT 
AAAGAGAGTG ACTCTCTGGT TCAGGCACTC AAGACATCAA GTAAGGTTGA TACATCTTTA 
ATCAGCAATA AGTCCACTGG TAATGAAACA ACTGAAATGC CCAAGAAGAG TAGAACCCAG 
ACACATTCAG AAAGGAAAAG ATTAAATGAA GATGGACTTC AGCTTGGTGA ACCACCAGCA 
AAGAAAGGGC TTATACTTAT TAGTCCACCC ATCACTGAAG ACCAAGACAA AAGGGAAGAA 
ATGCAACAGT CTGTTTCAGA AGGTGCAGAA GAAGACAGTA GAGTTTTGCA GGAAAAGAAT 
GAAGAGCTAA AAAGACTTTT AACTATTGGT GAGAATGAAC TCAGAAATGC AAAGGAGGAA 
AAGGCAGAAT TAAATAAACA GGTTGTGAGT TTGCAGCAGC AACTTTGTTT CTTTGAAGAA 
AAGAACTCAT CCCTGAGGGC AGAGGTAGAG CAAATCCAGG CCAGTTATGA CCTTGCAGCT 
GCTGAGCTGC ATACTCAGAG AGCTGTGAAC CAGGAGCAGA AGGACAGGAT CCTGCAATTG 
TCAGGGAAGA TGGAAACTGC GGCAAGACGT ATTGAAAGCA ATGTTTCACA GATAAAGCAA 
ATGCAAACAA AAATAGATGA ACTGCGATCT CTTGATTCAC CTTCTCATAT TTCAAAAATA 
GATTTACTCA ATCTCCAGGA TCTATCAAGT GGTGCTAATT TGCTGAACAC ATCACAACAG 
CTTCCAGGTA GTGACCTACC GAGCACATGG GTTAAAGAGT TTCATACTCA GGAGCTCAGC 
AGGGAAAGTT CCTTCCACTC TAGCATTGAA GCCATCTGGG AAGAATGTAA AGAGATTGTG 
AAGGCTTCCT CCAAAAAGTC TCATCAGATT CAGGGACTAG AAGAACTAAT TGAAAAATTA 
CAGGTGGAAG TAAAAAACTG CAGGGATGAG AACAGTGAGC TTCGAGCAAA GGAGAGTGAG 
GATAAGAATC GAGACCAGCA GCTGAAGGAG AAAGAAAGTC TAATCCAGCA GCTTAGAGAA 
GAACTTCAGG AAACCACTGT CAGTCTCCGT GTCCAAGTAC AGCTAGTAGC GGAAAGAGAG 
CAAGCTCTTT CAGAGCTTTC CCGGGATGTT ACCTGCTATA AGGCAAAAGT AAAGGACCTT 
GAAGTGATGG TAGAGACCCA GAAAGAGGAA TGTAAGCGCT TAGCTGAGTT AGAGCAGAGC 
ATTTTAGAGA AGGAATCTGC CATCCTAAAG CTGGAAGCGA GTCTAAAGGA ATTGGAAGCA 
AAGCACCAGG ATCACATCAG AAGTACCACA CATTTAAATG CAGAGGAAGT CAAGTTCAGA 
GAAGAAATTA CACAGTTGGC GAATAATTTG CATGATACAA AACAGTTACT TCAGTCAAAG 
GAAGAAGAAA ATGAAATTAG CAGACAAGAG ACAGAAAAGT TGAAAGAGGA GCTTGCTGCA 
AACTCTGTTC TTACCCAGAA TCTCCAAGCA GACCTTCAGC GGAAGGAGGA AGATTGTGCT 
GAGCTAAAGG AGAAGTTCAC TGATGCCAAA AAGCAGATCG AGCAGGTACA GAGGGAGGTA 
TCTGTAATGC GTGATGAAGA GAAGTCGTTG AGGACTAAAA TTAATGAACT GGAGAAAAAG 
AAAAACCAAT ATTCTCAGGA AATAGATATG AAACAACGAA CCATTCAGCA ACTTAAGGAG 
CAGTTAAGTA ATCAGAAAAT GGAAGAAGTT GTACAACAGT ATGAGAAAGT GTGCAAAGAT 
CTCAGCGTTA AGGAGAAGCT GATTGAAGCT ATGCGACTGA CACTAGTGGA GCAGGAGCAA 
ACTCAGGCAG AGCAGGACAG GATGCTTGAG GCTAAGTCAC AGGAAGCTGA CTGGCTAGCC 
GGAGAATTGG ATACATGGAA AGACAAATTC AAAGATCTGG AGACTAGAAG TAATCAAAAG 
GTAACTACAG AGGCCATGGA GGACAGCGAC GTGCTTAGTG AGAAGTTCAG GAAGCTTCAG 
GACGAGCTGC AGGAATCTGA AGAGAAACAT AAAGCTGATA GAAAGAAGTG GTTAGAAGAA 
AAAGCGGTTC TTACAACTCA AGCAAAAGAA GCAGAGACTC TACGAAACAG GGAGATGAAA 
AAATATGCTG AGGACAGGGA GCGTTGTCTG AAGCTGCAGA ATGAAGTGGA AACACTGACA 
GCACAGCTGG CGGAGAAAAC TGGTGAGCTT CAGAAGTGGA GAGAAGAGCG GGACCAGCTG 
GTCACAGCTG TGGAGACACA GATGCAGGCT CTGCTCTCCA GCAGCAAGCA TAAGGACGAA 
GAGATCCAGC AACTGAGAAA GGCCGTGGCA AAGAGCACGG GGACAGAAAA CCAGACCATG 
AATCTCAAGC CTGAATGTAA TGATTCAGTC GATCTTGGTG GAGTTGAAAC TGAACTTCAG 
TCAACAAGTT TTGAAATTTC TAGGAATACA GCAGAGGATG GATCTGTAGT TCTTGACTCT 
TGTGAAGTGT CAACAGAAAA TGTACAAAGT ACTCGATTTC CAAAGCCTGA ACTGGAGATT 
CAGTTTACAC CTCTGCAGCC AAACAAAGTG GCAGTGAAGC ACCCTGGTTG TGCCACACCA 
GTCACAATTA AGATCCCCAA GGCACGGAAG AGGAAGAGTG GCGAGGTGGA GGAGGACTTG 
GTGAAATGTG AAAATAAGAA GAATTCCACA CCCAGAAGTA ATGTGAAGTT TCCCGTTTCA 
GAGCATAGAA ATTCCTCTTT CAAAAAGGAA CAAAAGGTTT TCTTATGTCC GTCATCTAAG 
AAGACATACT CCTTACGGAG CCAAGCATCT ACGGTTGGTG CGAATGTAGC CTCTAAAAAA 
AAAGAGGGAA CACTACAGAA ATTTGGAGAC TTCTTACAAC ATTCTCCAAC AATCCTTCAA 
TCAAAAGCAA AAAAGATAAT TGAAACAATG AGTTCCCCAA AGTTATCAAC TGTAGAAGTA 
AGTAAAGAAA ATGTATCCCA GCCTAAAAAA GCCAAACGGA AATTGTACAG AAATGAAATT 
TCATCTCCCA TCAACATATC TGGCCAAGTG ATTTTAATGG AACAGAAAGC AAAAGAAACT 
GATCATCAGA TTCTCAAACG ACGGCTTCGA ACACGAACAG CCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif20b ( XM_006231286.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231348.1 Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X7, mRNA.

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CloneID	ORa24039
Clone ID Related Accession (Same CDS sequence)	XM_006231286.1
Accession Version	XM_006231286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF20B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA AACCACATAG ATGTAGAGAA TACCTACAGT TGTCATCTGA TCAAGAGAAA 
GAGGAAAGTG CTAACAAAAA TACATTGCTG CGACAAATTA AAGAGGTTAC CATACATAAT 
GACAGTTATG ACATTCTTTG TGGACGCTTA ACAAACTCTT TGACTATACC AGAATTTGAA 
GAAACCATGA ACAATTGTGA ACAATCTAGT CTCAATGTGG ATAATATAAA ATATTCAGTG 
TGGGTTTCTT TCTTTGAGAT TTACAATGAA AGTATTTATG ATTTGTTTGT TCCTGTGTCC 
TCTAAGTTGC AAAAGAGAAA AATGCTACGC CTTTCCCAAG ATGTAAAGGG CTATTCTTTT 
ATAAAAGATC TGCAGTGGAT CCAGGTATCT GATTCTAAAG AAGCATATAG ACTCTTAAAA 
CTAGGAGTGA AGCATCAGAG TGTTGCCTTC ACAAAACTGA ACAATGCTTC TAGCAGAAGT 
CACAGCATAT TCACTATTAG AATATTACAG ATAGAAGACT CGGAAATACC TCGTGTAACA 
CGAGTTAGTG AATTGTCGCT ATGTGATCTC GCTGGTTCAG AACGAAGCAT GAAGACGCAA 
AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG AATATCAACA CTTCTTTACT GACTCTGGGA 
AAGTGCATCA GTGTTCTGAA GAACAGTGAC AAGTCCAAGA TTCAACAACA TGTGCCTTTT 
CGAGAAAGTA AACTGACCCA CTATTTCCAA AGCTTTTTTA CTGGAAAGGG GAAAATATGT 
ATGATCATCA ATATTAGTCA GTGTTGTTCT GCCTATGATG AGACACTCAA TGTACTGAAG 
TTCTCAACTG TTGCACAAAA GGTCTATGTT CCAGATACTT TAAGTTCTTC TCAAGAGAAA 
TCGTTTGGGT CTACCAAATC TTTACAAGAT GTATCCCTAG GCAGTAATTT GGATAATAAA 
ATACTAAATG TAAAAAGAAA AACTGTTTCA TGGGAAAATA GTCTGGAAGA TGTGGTTGAA 
AATGAAGATT TGGTCGAGGA CTTAGAAGAA AATGAAGAAA CACAAAACAT GGAAACTGAA 
CTTACAGATG AAGATAGTGA TAAGCCGTTA GAGGAAGGCG GAGTCTGCGC AGGTCACGGG 
AAGAACAAGA AACTGTTGGA TTTAATAGAA AACTTGAAAA AAAGACTAAT AAATGAAAAA 
AAAGAAAAGT TAACATTGGA ATTTAAAATC CGAGAAGAAG TGACACAGGA ATTCACTCAG 
TATTGGAGCC AAAGGGAAGC TGATTTTAAG GAAACACTTC TTCATGAACG GGAGATATTA 
GAAGAAAATG CTGAACGTCG CTTAGCAATT TTCAAAGATT TGGTTGGAAA ACCTGGTGAG 
AGTCAAGATG AACCAGCAAG CAGATTTTGT ACCATGGAAC TTGAAACTGA AGAATCTCAT 
AATTATGTAG GAGTTGAAGA TATTTTTGAT TCTCTTGAAG ATGATGTCAC TGATATTAAG 
AAACAGGCCG AACTTGCTCA TTTGTATATT ACATCTCTTG TTGATCCCCA GGAGGCTATT 
GCTTGTTTGC AGCTAAAGTA TAATCAAGTT AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA 
TTAATTAAAG CCCAAGAAGA GCTAAAAAAT AAAGAGAGTG ACTCTCTGGT TCAGGCACTC 
AAGACATCAA GTAAGGTTGA TACATCTTTA ATCAGCAATA AGTCCACTGG TAATGAAACA 
ACTGAAATGC CCAAGAAGAG TAGAACCCAG ACACATTCAG AAAGGAAAAG ATTAAATGAA 
GATGGACTTC AGCTTGGTGA ACCACCAGCA AAGAAAGGGC TTATACTTAT TAGTCCACCC 
ATCACTGAAG ACCAAGACAA AAGGGAAGAA ATGCAACAGT CTGTTTCAGA AGGTGCAGAA 
GAAGACAGTA GAGTTTTGCA GGAAAAGAAT GAAGAGCTAA AAAGACTTTT AACTATTGGT 
GAGAATGAAC TCAGAAATGC AAAGGAGGAA AAGGCAGAAT TAAATAAACA GGTTGTGAGT 
TTGCAGCAGC AACTTTGTTT CTTTGAAGAA AAGAACTCAT CCCTGAGGGC AGAGGTAGAG 
CAAATCCAGG CCAGTTATGA CCTTGCAGCT GCTGAGCTGC ATACTCAGAG AGCTGTGAAC 
CAGGAGCAGA AGGACAGGAT CCTGCAATTG TCAGGGAAGA TGGAAACTGC GGCAAGACGT 
ATTGAAAGCA ATGTTTCACA GATAAAGCAA ATGCAAACAA AAATAGATGA ACTGCGATCT 
CTTGATTCAC CTTCTCATAT TTCAAAAATA GATTTACTCA ATCTCCAGGA TCTATCAAGT 
GGTGCTAATT TGCTGAACAC ATCACAACAG CTTCCAGGTA GTGACCTACC GAGCACATGG 
GTTAAAGAGT TTCATACTCA GGAGCTCAGC AGGGAAAGTT CCTTCCACTC TAGCATTGAA 
GCCATCTGGG AAGAATGTAA AGAGATTGTG AAGGCTTCCT CCAAAAAGTC TCATCAGATT 
CAGGGACTAG AAGAACTAAT TGAAAAATTA CAGGTGGAAG TAAAAAACTG CAGGGATGAG 
AACAGTGAGC TTCGAGCAAA GGAGAGTGAG GATAAGAATC GAGACCAGCA GCTGAAGGAG 
AAAGAAAGTC TAATCCAGCA GCTTAGAGAA GAACTTCAGG AAACCACTGT CAGTCTCCGT 
GTCCAAGTAC AGCTAGTAGC GGAAAGAGAG CAAGCTCTTT CAGAGCTTTC CCGGGATGTT 
ACCTGCTATA AGGCAAAAGT AAAGGACCTT GAAGTGATGG TAGAGACCCA GAAAGAGGAA 
TGTAAGCGCT TAGCTGAGTT AGAGCAGAGC ATTTTAGAGA AGGAATCTGC CATCCTAAAG 
CTGGAAGCGA GTCTAAAGGA ATTGGAAGCA AAGCACCAGG ATCACATCAG AAGTACCACA 
CATTTAAATG CAGAGGAAGT CAAGTTCAGA GAAGAAATTA CACAGTTGGC GAATAATTTG 
CATGATACAA AACAGTTACT TCAGTCAAAG GAAGAAGAAA ATGAAATTAG CAGACAAGAG 
ACAGAAAAGT TGAAAGAGGA GCTTGCTGCA AACTCTGTTC TTACCCAGAA TCTCCAAGCA 
GACCTTCAGC GGAAGGAGGA AGATTGTGCT GAGCTAAAGG AGAAGTTCAC TGATGCCAAA 
AAGCAGATCG AGCAGGTACA GAGGGAGGTA TCTGTAATGC GTGATGAAGA GAAGTCGTTG 
AGGACTAAAA TTAATGAACT GGAGAAAAAG AAAAACCAAT ATTCTCAGGA AATAGATATG 
AAACAACGAA CCATTCAGCA ACTTAAGGAG CAGTTAAGTA ATCAGAAAAT GGAAGAAGTT 
GTACAACAGT ATGAGAAAGT GTGCAAAGAT CTCAGCGTTA AGGAGAAGCT GATTGAAGCT 
ATGCGACTGA CACTAGTGGA GCAGGAGCAA ACTCAGGCAG AGCAGGACAG GATGCTTGAG 
GCTAAGTCAC AGGAAGCTGA CTGGCTAGCC GGAGAATTGG ATACATGGAA AGACAAATTC 
AAAGATCTGG AGACTAGAAG TAATCAAAAG GTAACTACAG AGGCCATGGA GGACAGCGAC 
GTGCTTAGTG AGAAGTTCAG GAAGCTTCAG GACGAGCTGC AGGAATCTGA AGAGAAACAT 
AAAGCTGATA GAAAGAAGTG GTTAGAAGAA AAAGCGGTTC TTACAACTCA AGCAAAAGAA 
GCAGAGACTC TACGAAACAG GGAGATGAAA AAATATGCTG AGGACAGGGA GCGTTGTCTG 
AAGCTGCAGA ATGAAGTGGA AACACTGACA GCACAGCTGG CGGAGAAAAC TGGTGAGCTT 
CAGAAGTGGA GAGAAGAGCG GGACCAGCTG GTCACAGCTG TGGAGACACA GATGCAGGCT 
CTGCTCTCCA GCAGCAAGCA TAAGGACGAA GAGATCCAGC AACTGAGAAA GGCCGTGGCA 
AAGAGCACGG GGACAGTGAG TGGACGCGTA CTCGAAAACC AGACCATGAA TCTCAAGCCT 
GAATGTAATG ATTCAGTCGA TCTTGGTGGA GTTGAAACTG AACTTCAGTC AACAAGTTTT 
GAAATTTCTA GGAATACAGC AGAGGATGGA TCTGTAGTTC TTGACTCTTG TGAAGTGTCA 
ACAGAAAATG TACAAAGTAC TCGATTTCCA AAGCCTGAAC TGGAGATTCA GTTTACACCT 
CTGCAGCCAA ACAAAGTGGC AGTGAAGCAC CCTGGTTGTG CCACACCAGT CACAATTAAG 
ATCCCCAAGG CACGGAAGAG GAAGAGTGGC GAGGTGGAGG AGGACTTGGT GAAATGTGAA 
AATAAGAAGA ATTCCACACC CAGAAGTAAT GTGAAGTTTC CCGTTTCAGA GCATAGAAAT 
TCCTCTTTCA AAAAGGAACA AAAGGTTTTC TTATGTCCGT CATCTAAGAA GACATACTCC 
TTACGGAGCC AAGCATCTAC GGTTGGTGCG AATGTAGCCT CTAAAAAAAA AGAGGGAACA 
CTACAGAAAT TTGGAGACTT CTTACAACAT TCTCCAACAA TCCTTCAATC AAAAGCAAAA 
AAGATAATTG AAACAATGAG TTCCCCAAAG TTATCAACTG TAGAAGTAAG TAAAGAAAAT 
GTATCCCAGC CTAAAAAAGC CAAACGGAAA TTGTACAGAA ATGAAATTTC ATCTCCCATC 
AACATATCTG GCCAAGTGAT TTTAATGGAA CAGAAAGCAA AAGAAACTGA TCATCAGATT 
CTCAAACGAC GGCTTCGAAC ACGAACAGCC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231348.1
CDS	195..4970
Translation	MSFKPHRCREYLQLSSDQEKEESANKNTLLRQIKEVTIHNDSYDILCGRLTNSLTIPEFEETMNNCEQSSLNVDNIKYSVWVSFFEIYNESIYDLFVPVSSKLQKRKMLRLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGVKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPRVTRVSELSLCDLAGSERSMKTQSEGERLREAGNINTSLLTLGKCISVLKNSDKSKIQQHVPFRESKLTHYFQSFFTGKGKICMIINISQCCSAYDETLNVLKFSTVAQKVYVPDTLSSSQEKSFGSTKSLQDVSLGSNLDNKILNVKRKTVSWENSLEDVVENEDLVEDLEENEETQNMETELTDEDSDKPLEEGGVCAGHGKNKKLLDLIENLKKRLINEKKEKLTLEFKIREEVTQEFTQYWSQREADFKETLLHEREILEENAERRLAIFKDLVGKPGESQDEPASRFCTMELETEESHNYVGVEDIFDSLEDDVTDIKKQAELAHLYITSLVDPQEAIACLQLKYNQVKAELAETKEELIKAQEELKNKESDSLVQALKTSSKVDTSLISNKSTGNETTEMPKKSRTQTHSERKRLNEDGLQLGEPPAKKGLILISPPITEDQDKREEMQQSVSEGAEEDSRVLQEKNEELKRLLTIGENELRNAKEEKAELNKQVVSLQQQLCFFEEKNSSLRAEVEQIQASYDLAAAELHTQRAVNQEQKDRILQLSGKMETAARRIESNVSQIKQMQTKIDELRSLDSPSHISKIDLLNLQDLSSGANLLNTSQQLPGSDLPSTWVKEFHTQELSRESSFHSSIEAIWEECKEIVKASSKKSHQIQGLEELIEKLQVEVKNCRDENSELRAKESEDKNRDQQLKEKESLIQQLREELQETTVSLRVQVQLVAEREQALSELSRDVTCYKAKVKDLEVMVETQKEECKRLAELEQSILEKESAILKLEASLKELEAKHQDHIRSTTHLNAEEVKFREEITQLANNLHDTKQLLQSKEEENEISRQETEKLKEELAANSVLTQNLQADLQRKEEDCAELKEKFTDAKKQIEQVQREVSVMRDEEKSLRTKINELEKKKNQYSQEIDMKQRTIQQLKEQLSNQKMEEVVQQYEKVCKDLSVKEKLIEAMRLTLVEQEQTQAEQDRMLEAKSQEADWLAGELDTWKDKFKDLETRSNQKVTTEAMEDSDVLSEKFRKLQDELQESEEKHKADRKKWLEEKAVLTTQAKEAETLRNREMKKYAEDRERCLKLQNEVETLTAQLAEKTGELQKWREERDQLVTAVETQMQALLSSSKHKDEEIQQLRKAVAKSTGTVSGRVLENQTMNLKPECNDSVDLGGVETELQSTSFEISRNTAEDGSVVLDSCEVSTENVQSTRFPKPELEIQFTPLQPNKVAVKHPGCATPVTIKIPKARKRKSGEVEEDLVKCENKKNSTPRSNVKFPVSEHRNSSFKKEQKVFLCPSSKKTYSLRSQASTVGANVASKKKEGTLQKFGDFLQHSPTILQSKAKKIIETMSSPKLSTVEVSKENVSQPKKAKRKLYRNEISSPINISGQVILMEQKAKETDHQILKRRLRTRTAK

Target ORF information:

RefSeq Version	XM_006231286.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231286.1

ORF Insert Sequence:

ATGAGCTTCA AACCACATAG ATGTAGAGAA TACCTACAGT TGTCATCTGA TCAAGAGAAA 
GAGGAAAGTG CTAACAAAAA TACATTGCTG CGACAAATTA AAGAGGTTAC CATACATAAT 
GACAGTTATG ACATTCTTTG TGGACGCTTA ACAAACTCTT TGACTATACC AGAATTTGAA 
GAAACCATGA ACAATTGTGA ACAATCTAGT CTCAATGTGG ATAATATAAA ATATTCAGTG 
TGGGTTTCTT TCTTTGAGAT TTACAATGAA AGTATTTATG ATTTGTTTGT TCCTGTGTCC 
TCTAAGTTGC AAAAGAGAAA AATGCTACGC CTTTCCCAAG ATGTAAAGGG CTATTCTTTT 
ATAAAAGATC TGCAGTGGAT CCAGGTATCT GATTCTAAAG AAGCATATAG ACTCTTAAAA 
CTAGGAGTGA AGCATCAGAG TGTTGCCTTC ACAAAACTGA ACAATGCTTC TAGCAGAAGT 
CACAGCATAT TCACTATTAG AATATTACAG ATAGAAGACT CGGAAATACC TCGTGTAACA 
CGAGTTAGTG AATTGTCGCT ATGTGATCTC GCTGGTTCAG AACGAAGCAT GAAGACGCAA 
AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG AATATCAACA CTTCTTTACT GACTCTGGGA 
AAGTGCATCA GTGTTCTGAA GAACAGTGAC AAGTCCAAGA TTCAACAACA TGTGCCTTTT 
CGAGAAAGTA AACTGACCCA CTATTTCCAA AGCTTTTTTA CTGGAAAGGG GAAAATATGT 
ATGATCATCA ATATTAGTCA GTGTTGTTCT GCCTATGATG AGACACTCAA TGTACTGAAG 
TTCTCAACTG TTGCACAAAA GGTCTATGTT CCAGATACTT TAAGTTCTTC TCAAGAGAAA 
TCGTTTGGGT CTACCAAATC TTTACAAGAT GTATCCCTAG GCAGTAATTT GGATAATAAA 
ATACTAAATG TAAAAAGAAA AACTGTTTCA TGGGAAAATA GTCTGGAAGA TGTGGTTGAA 
AATGAAGATT TGGTCGAGGA CTTAGAAGAA AATGAAGAAA CACAAAACAT GGAAACTGAA 
CTTACAGATG AAGATAGTGA TAAGCCGTTA GAGGAAGGCG GAGTCTGCGC AGGTCACGGG 
AAGAACAAGA AACTGTTGGA TTTAATAGAA AACTTGAAAA AAAGACTAAT AAATGAAAAA 
AAAGAAAAGT TAACATTGGA ATTTAAAATC CGAGAAGAAG TGACACAGGA ATTCACTCAG 
TATTGGAGCC AAAGGGAAGC TGATTTTAAG GAAACACTTC TTCATGAACG GGAGATATTA 
GAAGAAAATG CTGAACGTCG CTTAGCAATT TTCAAAGATT TGGTTGGAAA ACCTGGTGAG 
AGTCAAGATG AACCAGCAAG CAGATTTTGT ACCATGGAAC TTGAAACTGA AGAATCTCAT 
AATTATGTAG GAGTTGAAGA TATTTTTGAT TCTCTTGAAG ATGATGTCAC TGATATTAAG 
AAACAGGCCG AACTTGCTCA TTTGTATATT ACATCTCTTG TTGATCCCCA GGAGGCTATT 
GCTTGTTTGC AGCTAAAGTA TAATCAAGTT AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA 
TTAATTAAAG CCCAAGAAGA GCTAAAAAAT AAAGAGAGTG ACTCTCTGGT TCAGGCACTC 
AAGACATCAA GTAAGGTTGA TACATCTTTA ATCAGCAATA AGTCCACTGG TAATGAAACA 
ACTGAAATGC CCAAGAAGAG TAGAACCCAG ACACATTCAG AAAGGAAAAG ATTAAATGAA 
GATGGACTTC AGCTTGGTGA ACCACCAGCA AAGAAAGGGC TTATACTTAT TAGTCCACCC 
ATCACTGAAG ACCAAGACAA AAGGGAAGAA ATGCAACAGT CTGTTTCAGA AGGTGCAGAA 
GAAGACAGTA GAGTTTTGCA GGAAAAGAAT GAAGAGCTAA AAAGACTTTT AACTATTGGT 
GAGAATGAAC TCAGAAATGC AAAGGAGGAA AAGGCAGAAT TAAATAAACA GGTTGTGAGT 
TTGCAGCAGC AACTTTGTTT CTTTGAAGAA AAGAACTCAT CCCTGAGGGC AGAGGTAGAG 
CAAATCCAGG CCAGTTATGA CCTTGCAGCT GCTGAGCTGC ATACTCAGAG AGCTGTGAAC 
CAGGAGCAGA AGGACAGGAT CCTGCAATTG TCAGGGAAGA TGGAAACTGC GGCAAGACGT 
ATTGAAAGCA ATGTTTCACA GATAAAGCAA ATGCAAACAA AAATAGATGA ACTGCGATCT 
CTTGATTCAC CTTCTCATAT TTCAAAAATA GATTTACTCA ATCTCCAGGA TCTATCAAGT 
GGTGCTAATT TGCTGAACAC ATCACAACAG CTTCCAGGTA GTGACCTACC GAGCACATGG 
GTTAAAGAGT TTCATACTCA GGAGCTCAGC AGGGAAAGTT CCTTCCACTC TAGCATTGAA 
GCCATCTGGG AAGAATGTAA AGAGATTGTG AAGGCTTCCT CCAAAAAGTC TCATCAGATT 
CAGGGACTAG AAGAACTAAT TGAAAAATTA CAGGTGGAAG TAAAAAACTG CAGGGATGAG 
AACAGTGAGC TTCGAGCAAA GGAGAGTGAG GATAAGAATC GAGACCAGCA GCTGAAGGAG 
AAAGAAAGTC TAATCCAGCA GCTTAGAGAA GAACTTCAGG AAACCACTGT CAGTCTCCGT 
GTCCAAGTAC AGCTAGTAGC GGAAAGAGAG CAAGCTCTTT CAGAGCTTTC CCGGGATGTT 
ACCTGCTATA AGGCAAAAGT AAAGGACCTT GAAGTGATGG TAGAGACCCA GAAAGAGGAA 
TGTAAGCGCT TAGCTGAGTT AGAGCAGAGC ATTTTAGAGA AGGAATCTGC CATCCTAAAG 
CTGGAAGCGA GTCTAAAGGA ATTGGAAGCA AAGCACCAGG ATCACATCAG AAGTACCACA 
CATTTAAATG CAGAGGAAGT CAAGTTCAGA GAAGAAATTA CACAGTTGGC GAATAATTTG 
CATGATACAA AACAGTTACT TCAGTCAAAG GAAGAAGAAA ATGAAATTAG CAGACAAGAG 
ACAGAAAAGT TGAAAGAGGA GCTTGCTGCA AACTCTGTTC TTACCCAGAA TCTCCAAGCA 
GACCTTCAGC GGAAGGAGGA AGATTGTGCT GAGCTAAAGG AGAAGTTCAC TGATGCCAAA 
AAGCAGATCG AGCAGGTACA GAGGGAGGTA TCTGTAATGC GTGATGAAGA GAAGTCGTTG 
AGGACTAAAA TTAATGAACT GGAGAAAAAG AAAAACCAAT ATTCTCAGGA AATAGATATG 
AAACAACGAA CCATTCAGCA ACTTAAGGAG CAGTTAAGTA ATCAGAAAAT GGAAGAAGTT 
GTACAACAGT ATGAGAAAGT GTGCAAAGAT CTCAGCGTTA AGGAGAAGCT GATTGAAGCT 
ATGCGACTGA CACTAGTGGA GCAGGAGCAA ACTCAGGCAG AGCAGGACAG GATGCTTGAG 
GCTAAGTCAC AGGAAGCTGA CTGGCTAGCC GGAGAATTGG ATACATGGAA AGACAAATTC 
AAAGATCTGG AGACTAGAAG TAATCAAAAG GTAACTACAG AGGCCATGGA GGACAGCGAC 
GTGCTTAGTG AGAAGTTCAG GAAGCTTCAG GACGAGCTGC AGGAATCTGA AGAGAAACAT 
AAAGCTGATA GAAAGAAGTG GTTAGAAGAA AAAGCGGTTC TTACAACTCA AGCAAAAGAA 
GCAGAGACTC TACGAAACAG GGAGATGAAA AAATATGCTG AGGACAGGGA GCGTTGTCTG 
AAGCTGCAGA ATGAAGTGGA AACACTGACA GCACAGCTGG CGGAGAAAAC TGGTGAGCTT 
CAGAAGTGGA GAGAAGAGCG GGACCAGCTG GTCACAGCTG TGGAGACACA GATGCAGGCT 
CTGCTCTCCA GCAGCAAGCA TAAGGACGAA GAGATCCAGC AACTGAGAAA GGCCGTGGCA 
AAGAGCACGG GGACAGTGAG TGGACGCGTA CTCGAAAACC AGACCATGAA TCTCAAGCCT 
GAATGTAATG ATTCAGTCGA TCTTGGTGGA GTTGAAACTG AACTTCAGTC AACAAGTTTT 
GAAATTTCTA GGAATACAGC AGAGGATGGA TCTGTAGTTC TTGACTCTTG TGAAGTGTCA 
ACAGAAAATG TACAAAGTAC TCGATTTCCA AAGCCTGAAC TGGAGATTCA GTTTACACCT 
CTGCAGCCAA ACAAAGTGGC AGTGAAGCAC CCTGGTTGTG CCACACCAGT CACAATTAAG 
ATCCCCAAGG CACGGAAGAG GAAGAGTGGC GAGGTGGAGG AGGACTTGGT GAAATGTGAA 
AATAAGAAGA ATTCCACACC CAGAAGTAAT GTGAAGTTTC CCGTTTCAGA GCATAGAAAT 
TCCTCTTTCA AAAAGGAACA AAAGGTTTTC TTATGTCCGT CATCTAAGAA GACATACTCC 
TTACGGAGCC AAGCATCTAC GGTTGGTGCG AATGTAGCCT CTAAAAAAAA AGAGGGAACA 
CTACAGAAAT TTGGAGACTT CTTACAACAT TCTCCAACAA TCCTTCAATC AAAAGCAAAA 
AAGATAATTG AAACAATGAG TTCCCCAAAG TTATCAACTG TAGAAGTAAG TAAAGAAAAT 
GTATCCCAGC CTAAAAAAGC CAAACGGAAA TTGTACAGAA ATGAAATTTC ATCTCCCATC 
AACATATCTG GCCAAGTGAT TTTAATGGAA CAGAAAGCAA AAGAAACTGA TCATCAGATT 
CTCAAACGAC GGCTTCGAAC ACGAACAGCC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif20b ( XM_008760329.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758551.1 Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X3, mRNA.

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CloneID	ORa46765
Clone ID Related Accession (Same CDS sequence)	XM_008760329.2
Accession Version	XM_008760329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF20B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760329.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTC ATTTGAATCA AGACGGAGTG CCTAGACCAT CTTATGTTTT TAGTGCTGAC 
CCAATTGCAA GGCCTTCGGA AATCAATTTT GATGGCGTTA AGCTTGATCT CTCTCATGAG 
TTTTCCTTAG TTGCCGCAAA CCCTGGGGCA AACAGTTTGG AATCTAAAAA TTACCTCCAG 
GTTTGTCTTC GAATAAGACC ATTTACACAG TCGGAAAAAG GACACGAGGC TGAGGGCTGT 
GTGCAAGTTC TGGATTCACA GACTGTTCTG CTGAAAGACC CTCAAAGCAT CCTTGGCCAC 
TTAAGTGAGA AGAGCTCCGG ACAGATGGCA CAGAAATTCA GCTTTTCTAG GGTTTTTGGA 
CCCGAAACTT CACAGAAAGA GTTCTTTCAG GGTTGCATTA TGCAGCCAGT AAAAGACCTC 
TTAAAAGGGC ACAGTCGACT GATCTTCACA TATGGGCTGA CCAATTCTGG AAAAACATAT 
ACATTTCAAG GCACAGAAGA AAACATTGGC ATTCTGCCTA GAACACTGAA TGTATTGTTT 
GATAGTCTTC AGGAAAGACT CTATACAAAG ATGAGCTTCA AACCACATAG ATGTAGAGAA 
TACCTACAGT TGTCATCTGA TCAAGAGAAA GAGGAAAGTG CTAACAAAAA TACATTGCTG 
CGACAAATTA AAGAGGTTAC CATACATAAT GACAGTTATG ACATTCTTTG TGGACGCTTA 
ACAAACTCTT TGACTATACC AGAATTTGAA GAAACCATGA ACAATTGTGA ACAATCTAGT 
CTCAATGTGG ATAATATAAA ATATTCAGTG TGGGTTTCTT TCTTTGAGAT TTACAATGAA 
AGTATTTATG ATTTGTTTGT TCCTGTGTCC TCTAAGTTGC AAAAGAGAAA AATGCTACGC 
CTTTCCCAAG ATGTAAAGGG CTATTCTTTT ATAAAAGATC TGCAGTGGAT CCAGGTATCT 
GATTCTAAAG AAGCATATAG ACTCTTAAAA CTAGGAGTGA AGCATCAGAG TGTTGCCTTC 
ACAAAACTGA ACAATGCTTC TAGCAGAAGT CACAGCATAT TCACTATTAG AATATTACAG 
ATAGAAGACT CGGAAATACC TCGTGTAACA CGAGTTAGTG AATTGTCGCT ATGTGATCTC 
GCTGGTTCAG AACGAAGCAT GAAGACGCAA AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG 
AATATCAACA CTTCTTTACT GACTCTGGGA AAGTGCATCA GTGTTCTGAA GAACAGTGAC 
AAGTCCAAGA TTCAACAACA TGTGCCTTTT CGAGAAAGTA AACTGACCCA CTATTTCCAA 
AGCTTTTTTA CTGGAAAGGG GAAAATATGT ATGATCATCA ATATTAGTCA GTGTTGTTCT 
GCCTATGATG AGACACTCAA TGTACTGAAG TTCTCAACTG TTGCACAAAA GGTCTATGTT 
CCAGATACTT TAAGTTCTTC TCAAGAGAAA TCGTTTGGGT CTACCAAATC TTTACAAGAT 
GTATCCCTAG GCAGTAATTT GGATAATAAA ATACTAAATG TAAAAAGAAA AACTGTTTCA 
TGGGAAAATA GTCTGGAAGA TGTGGTTGAA AATGAAGATT TGGTCGAGGA CTTAGAAGAA 
AATGAAGAAA CACAAAACAT GGAAACTGAA CTTACAGATG AAGATAGTGA TAAGCCGTTA 
GAGGAAGGCG GAGTCTGCGC AGGTCACGGG AAGAACAAGA AACTGTTGGA TTTAATAGAA 
AACTTGAAAA AAAGACTAAT AAATGAAAAA AAAGAAAAGT TAACATTGGA ATTTAAAATC 
CGAGAAGAAG TGACACAGGA ATTCACTCAG TATTGGAGCC AAAGGGAAGC TGATTTTAAG 
GAAACACTTC TTCATGAACG GGAGATATTA GAAGAAAATG CTGAACGTCG CTTAGCAATT 
TTCAAAGATT TGGTTGGAAA ACCTGGTGAG AGTCAAGATG AACCAGCAAG CAGATTTTGT 
ACCATGGAAC TTGAAACTGA AGAATCTCAT AATTATGTAG GAGTTGAAGA TATTTTTGAT 
TCTCTTGAAG ATGATGTCAC TGATATTAAG AAACAGGCCG AACTTGCTCA TTTGTATATT 
ACATCTCTTG TTGATCCCCA GGAGGCTATT GCTTGTTTGC AGCTAAAGTA TAATCAAGTT 
AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA TTAATTAAAG CCCAAGAAGA GCTAAAAAAT 
AAAGAGAGTG ACTCTCTGGT TCAGGCACTC AAGACATCAA GTAAGGTTGA TACATCTTTA 
ATCAGCAATA AGTCCACTGG TAATGAAACA ACTGAAATGC CCAAGAAGAG TAGAACCCAG 
ACACATTCAG AAAGGAAAAG ATTAAATGAA GATGGACTTC AGCTTGGTGA ACCACCAGCA 
AAGAAAGGGC TTATACTTAT TAGTCCACCC ATCACTGAAG ACCAAGACAA AAGGGAAGAA 
ATGCAACAGT CTGTTTCAGA AGGTGCAGAA GAAGACAGTA GAGTTTTGCA GGAAAAGAAT 
GAAGAGCTAA AAAGACTTTT AACTATTGGT GAGAATGAAC TCAGAAATGC AAAGGAGGAA 
AAGGCAGAAT TAAATAAACA GGTTGTGAGT TTGCAGCAGC AACTTTGTTT CTTTGAAGAA 
AAGAACTCAT CCCTGAGGGC AGAGGTAGAG CAAATCCAGG CCAGTTATGA CCTTGCAGCT 
GCTGAGCTGC ATACTCAGAG AGCTGTGAAC CAGGAGCAGA AGGACAGGAT CCTGCAATTG 
TCAGGGAAGA TGGAAACTGC GGCAAGACGT ATTGAAAGCA ATGTTTCACA GATAAAGCAA 
ATGCAAACAA AAATAGATGA ACTGCGATCT CTTGATTCAC CTTCTCATAT TTCAAAAATA 
GATTTACTCA ATCTCCAGGA TCTATCAAGT GGTGCTAATT TGCTGAACAC ATCACAACAG 
CTTCCAGGTA GTGACCTACC GAGCACATGG GTTAAAGAGT TTCATACTCA GGAGCTCAGC 
AGGGAAAGTT CCTTCCACTC TAGCATTGAA GCCATCTGGG AAGAATGTAA AGAGATTGTG 
AAGGCTTCCT CCAAAAAGTC TCATCAGATT CAGGGACTAG AAGAACTAAT TGAAAAATTA 
CAGGTGGAAG TAAAAAACTG CAGGGATGAG AACAGTGAGC TTCGAGCAAA GGAGAGTGAG 
GATAAGAATC GAGACCAGCA GCTGAAGGAG AAAGAAAGTC TAATCCAGCA GCTTAGAGAA 
GAACTTCAGG AAACCACTGT CAGTCTCCGT GTCCAAGTAC AGCTAGTAGC GGAAAGAGAG 
CAAGCTCTTT CAGAGCTTTC CCGGGATGTT ACCTGCTATA AGGCAAAAGT AAAGGACCTT 
GAAGTGATGG TAGAGACCCA GAAAGAGGAA TGTAAGCGCT TAGCTGAGTT AGAGCAGAGC 
ATTTTAGAGA AGGAATCTGC CATCCTAAAG CTGGAAGCGA GTCTAAAGGA ATTGGAAGCA 
AAGCACCAGG ATCACATCAG AAGTACCACA CATTTAAATG CAGAGGAAGT CAAGTTCAGA 
GAAGAAATTA CACAGTTGGC GAATAATTTG CATGATACAA AACAGTTACT TCAGTCAAAG 
GAAGAAGAAA ATGAAATTAG CAGACAAGAG ACAGAAAAGT TGAAAGAGGA GCTTGCTGCA 
AACTCTGTTC TTACCCAGAA TCTCCAAGCA GACCTTCAGC GGAAGGAGGA AGATTGTGCT 
GAGCTAAAGG AGAAGTTCAC TGATGCCAAA AAGCAGATCG AGCAGGTATC TGTAATGCGT 
GATGAAGAGA AGTCGTTGAG GACTAAAATT AATGAACTGG AGAAAAAGAA AAACCAATAT 
TCTCAGGAAA TAGATATGAA ACAACGAACC ATTCAGCAAC TTAAGGAGCA GTTAAGTAAT 
CAGAAAATGG AAGAAGTTGT ACAACAGTAT GAGAAAGTGT GCAAAGATCT CAGCGTTAAG 
GAGAAGCTGA TTGAAGCTAT GCGACTGACA CTAGTGGAGC AGGAGCAAAC TCAGGCAGAG 
CAGGACAGGA TGCTTGAGGC TAAGTCACAG GAAGCTGACT GGCTAGCCGG AGAATTGGAT 
ACATGGAAAG ACAAATTCAA AGATCTGGAG ACTAGAAGTA ATCAAAAGGT AACTACAGAG 
GCCATGGAGG ACAGCGACGT GCTTAGTGAG AAGTTCAGGA AGCTTCAGGA CGAGCTGCAG 
GAATCTGAAG AGAAACATAA AGCTGATAGA AAGAAGTGGT TAGAAGAAAA AGCGGTTCTT 
ACAACTCAAG CAAAAGAAGC AGAGACTCTA CGAAACAGGG AGATGAAAAA ATATGCTGAG 
GACAGGGAGC GTTGTCTGAA GCTGCAGAAT GAAGTGGAAA CACTGACAGC ACAGCTGGCG 
GAGAAAACTG GTGAGCTTCA GAAGTGGAGA GAAGAGCGGG ACCAGCTGGT CACAGCTGTG 
GAGACACAGA TGCAGGCTCT GCTCTCCAGC AGCAAGCATA AGGACGAAGA GATCCAGCAA 
CTGAGAAAGG CCGTGGCAAA GAGCACGGGG ACAGTGAGTG GACGCGTACT CGAAAACCAG 
ACCATGAATC TCAAGCCTGA ATGTAATGAT TCAGTCGATC TTGGTGGAGT TGAAACTGAA 
CTTCAGTCAA CAAGTTTTGA AATTTCTAGG AATACAGCAG AGGATGGATC TGTAGTTCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATGTA CAAAGTACTC GATTTCCAAA GCCTGAACTG 
GAGATTCAGT TTACACCTCT GCAGCCAAAC AAAGTGGCAG TGAAGCACCC TGGTTGTGCC 
ACACCAGTCA CAATTAAGAT CCCCAAGGCA CGGAAGAGGA AGAGTGGCGA GGTGGAGGAG 
GACTTGGTGA AATGTGAAAA TAAGAAGAAT TCCACACCCA GAAGTAATGT GAAGTTTCCC 
GTTTCAGAGC ATAGAAATTC CTCTTTCAAA AAGGAACAAA AGGTTTTCTT ATGTCCGTCA 
TCTAAGAAGA CATACTCCTT ACGGAGCCAA GCATCTACGG TTGGTGCGAA TGTAGCCTCT 
AAAAAAAAAG AGGGAACACT ACAGAAATTT GGAGACTTCT TACAACATTC TCCAACAATC 
CTTCAATCAA AAGCAAAAAA GATAATTGAA ACAATGAGTT CCCCAAAGTT ATCAACTGTA 
GAAGTAAGTA AAGAAAATGT ATCCCAGCCT AAAAAAGCCA AACGGAAATT GTACAGAAAT 
GAAATTTCAT CTCCCATCAA CATATCTGGC CAAGTGATTT TAATGGAACA GAAAGCAAAA 
GAAACTGATC ATCAGATTCT CAAACGACGG CTTCGAACAC GAACAGCCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758551.1
CDS	120..5453
Translation	MESHLNQDGVPRPSYVFSADPIARPSEINFDGVKLDLSHEFSLVAANPGANSLESKNYLQVCLRIRPFTQSEKGHEAEGCVQVLDSQTVLLKDPQSILGHLSEKSSGQMAQKFSFSRVFGPETSQKEFFQGCIMQPVKDLLKGHSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLFDSLQERLYTKMSFKPHRCREYLQLSSDQEKEESANKNTLLRQIKEVTIHNDSYDILCGRLTNSLTIPEFEETMNNCEQSSLNVDNIKYSVWVSFFEIYNESIYDLFVPVSSKLQKRKMLRLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGVKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPRVTRVSELSLCDLAGSERSMKTQSEGERLREAGNINTSLLTLGKCISVLKNSDKSKIQQHVPFRESKLTHYFQSFFTGKGKICMIINISQCCSAYDETLNVLKFSTVAQKVYVPDTLSSSQEKSFGSTKSLQDVSLGSNLDNKILNVKRKTVSWENSLEDVVENEDLVEDLEENEETQNMETELTDEDSDKPLEEGGVCAGHGKNKKLLDLIENLKKRLINEKKEKLTLEFKIREEVTQEFTQYWSQREADFKETLLHEREILEENAERRLAIFKDLVGKPGESQDEPASRFCTMELETEESHNYVGVEDIFDSLEDDVTDIKKQAELAHLYITSLVDPQEAIACLQLKYNQVKAELAETKEELIKAQEELKNKESDSLVQALKTSSKVDTSLISNKSTGNETTEMPKKSRTQTHSERKRLNEDGLQLGEPPAKKGLILISPPITEDQDKREEMQQSVSEGAEEDSRVLQEKNEELKRLLTIGENELRNAKEEKAELNKQVVSLQQQLCFFEEKNSSLRAEVEQIQASYDLAAAELHTQRAVNQEQKDRILQLSGKMETAARRIESNVSQIKQMQTKIDELRSLDSPSHISKIDLLNLQDLSSGANLLNTSQQLPGSDLPSTWVKEFHTQELSRESSFHSSIEAIWEECKEIVKASSKKSHQIQGLEELIEKLQVEVKNCRDENSELRAKESEDKNRDQQLKEKESLIQQLREELQETTVSLRVQVQLVAEREQALSELSRDVTCYKAKVKDLEVMVETQKEECKRLAELEQSILEKESAILKLEASLKELEAKHQDHIRSTTHLNAEEVKFREEITQLANNLHDTKQLLQSKEEENEISRQETEKLKEELAANSVLTQNLQADLQRKEEDCAELKEKFTDAKKQIEQVSVMRDEEKSLRTKINELEKKKNQYSQEIDMKQRTIQQLKEQLSNQKMEEVVQQYEKVCKDLSVKEKLIEAMRLTLVEQEQTQAEQDRMLEAKSQEADWLAGELDTWKDKFKDLETRSNQKVTTEAMEDSDVLSEKFRKLQDELQESEEKHKADRKKWLEEKAVLTTQAKEAETLRNREMKKYAEDRERCLKLQNEVETLTAQLAEKTGELQKWREERDQLVTAVETQMQALLSSSKHKDEEIQQLRKAVAKSTGTVSGRVLENQTMNLKPECNDSVDLGGVETELQSTSFEISRNTAEDGSVVLDSCEVSTENVQSTRFPKPELEIQFTPLQPNKVAVKHPGCATPVTIKIPKARKRKSGEVEEDLVKCENKKNSTPRSNVKFPVSEHRNSSFKKEQKVFLCPSSKKTYSLRSQASTVGANVASKKKEGTLQKFGDFLQHSPTILQSKAKKIIETMSSPKLSTVEVSKENVSQPKKAKRKLYRNEISSPINISGQVILMEQKAKETDHQILKRRLRTRTAK

Target ORF information:

RefSeq Version	XM_008760329.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 20B (Kif20b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760329.2

ORF Insert Sequence:

ATGGAATCTC ATTTGAATCA AGACGGAGTG CCTAGACCAT CTTATGTTTT TAGTGCTGAC 
CCAATTGCAA GGCCTTCGGA AATCAATTTT GATGGCGTTA AGCTTGATCT CTCTCATGAG 
TTTTCCTTAG TTGCCGCAAA CCCTGGGGCA AACAGTTTGG AATCTAAAAA TTACCTCCAG 
GTTTGTCTTC GAATAAGACC ATTTACACAG TCGGAAAAAG GACACGAGGC TGAGGGCTGT 
GTGCAAGTTC TGGATTCACA GACTGTTCTG CTGAAAGACC CTCAAAGCAT CCTTGGCCAC 
TTAAGTGAGA AGAGCTCCGG ACAGATGGCA CAGAAATTCA GCTTTTCTAG GGTTTTTGGA 
CCCGAAACTT CACAGAAAGA GTTCTTTCAG GGTTGCATTA TGCAGCCAGT AAAAGACCTC 
TTAAAAGGGC ACAGTCGACT GATCTTCACA TATGGGCTGA CCAATTCTGG AAAAACATAT 
ACATTTCAAG GCACAGAAGA AAACATTGGC ATTCTGCCTA GAACACTGAA TGTATTGTTT 
GATAGTCTTC AGGAAAGACT CTATACAAAG ATGAGCTTCA AACCACATAG ATGTAGAGAA 
TACCTACAGT TGTCATCTGA TCAAGAGAAA GAGGAAAGTG CTAACAAAAA TACATTGCTG 
CGACAAATTA AAGAGGTTAC CATACATAAT GACAGTTATG ACATTCTTTG TGGACGCTTA 
ACAAACTCTT TGACTATACC AGAATTTGAA GAAACCATGA ACAATTGTGA ACAATCTAGT 
CTCAATGTGG ATAATATAAA ATATTCAGTG TGGGTTTCTT TCTTTGAGAT TTACAATGAA 
AGTATTTATG ATTTGTTTGT TCCTGTGTCC TCTAAGTTGC AAAAGAGAAA AATGCTACGC 
CTTTCCCAAG ATGTAAAGGG CTATTCTTTT ATAAAAGATC TGCAGTGGAT CCAGGTATCT 
GATTCTAAAG AAGCATATAG ACTCTTAAAA CTAGGAGTGA AGCATCAGAG TGTTGCCTTC 
ACAAAACTGA ACAATGCTTC TAGCAGAAGT CACAGCATAT TCACTATTAG AATATTACAG 
ATAGAAGACT CGGAAATACC TCGTGTAACA CGAGTTAGTG AATTGTCGCT ATGTGATCTC 
GCTGGTTCAG AACGAAGCAT GAAGACGCAA AGCGAAGGAG AAAGGCTAAG AGAGGCTGGG 
AATATCAACA CTTCTTTACT GACTCTGGGA AAGTGCATCA GTGTTCTGAA GAACAGTGAC 
AAGTCCAAGA TTCAACAACA TGTGCCTTTT CGAGAAAGTA AACTGACCCA CTATTTCCAA 
AGCTTTTTTA CTGGAAAGGG GAAAATATGT ATGATCATCA ATATTAGTCA GTGTTGTTCT 
GCCTATGATG AGACACTCAA TGTACTGAAG TTCTCAACTG TTGCACAAAA GGTCTATGTT 
CCAGATACTT TAAGTTCTTC TCAAGAGAAA TCGTTTGGGT CTACCAAATC TTTACAAGAT 
GTATCCCTAG GCAGTAATTT GGATAATAAA ATACTAAATG TAAAAAGAAA AACTGTTTCA 
TGGGAAAATA GTCTGGAAGA TGTGGTTGAA AATGAAGATT TGGTCGAGGA CTTAGAAGAA 
AATGAAGAAA CACAAAACAT GGAAACTGAA CTTACAGATG AAGATAGTGA TAAGCCGTTA 
GAGGAAGGCG GAGTCTGCGC AGGTCACGGG AAGAACAAGA AACTGTTGGA TTTAATAGAA 
AACTTGAAAA AAAGACTAAT AAATGAAAAA AAAGAAAAGT TAACATTGGA ATTTAAAATC 
CGAGAAGAAG TGACACAGGA ATTCACTCAG TATTGGAGCC AAAGGGAAGC TGATTTTAAG 
GAAACACTTC TTCATGAACG GGAGATATTA GAAGAAAATG CTGAACGTCG CTTAGCAATT 
TTCAAAGATT TGGTTGGAAA ACCTGGTGAG AGTCAAGATG AACCAGCAAG CAGATTTTGT 
ACCATGGAAC TTGAAACTGA AGAATCTCAT AATTATGTAG GAGTTGAAGA TATTTTTGAT 
TCTCTTGAAG ATGATGTCAC TGATATTAAG AAACAGGCCG AACTTGCTCA TTTGTATATT 
ACATCTCTTG TTGATCCCCA GGAGGCTATT GCTTGTTTGC AGCTAAAGTA TAATCAAGTT 
AAAGCAGAAT TAGCTGAAAC TAAAGAAGAA TTAATTAAAG CCCAAGAAGA GCTAAAAAAT 
AAAGAGAGTG ACTCTCTGGT TCAGGCACTC AAGACATCAA GTAAGGTTGA TACATCTTTA 
ATCAGCAATA AGTCCACTGG TAATGAAACA ACTGAAATGC CCAAGAAGAG TAGAACCCAG 
ACACATTCAG AAAGGAAAAG ATTAAATGAA GATGGACTTC AGCTTGGTGA ACCACCAGCA 
AAGAAAGGGC TTATACTTAT TAGTCCACCC ATCACTGAAG ACCAAGACAA AAGGGAAGAA 
ATGCAACAGT CTGTTTCAGA AGGTGCAGAA GAAGACAGTA GAGTTTTGCA GGAAAAGAAT 
GAAGAGCTAA AAAGACTTTT AACTATTGGT GAGAATGAAC TCAGAAATGC AAAGGAGGAA 
AAGGCAGAAT TAAATAAACA GGTTGTGAGT TTGCAGCAGC AACTTTGTTT CTTTGAAGAA 
AAGAACTCAT CCCTGAGGGC AGAGGTAGAG CAAATCCAGG CCAGTTATGA CCTTGCAGCT 
GCTGAGCTGC ATACTCAGAG AGCTGTGAAC CAGGAGCAGA AGGACAGGAT CCTGCAATTG 
TCAGGGAAGA TGGAAACTGC GGCAAGACGT ATTGAAAGCA ATGTTTCACA GATAAAGCAA 
ATGCAAACAA AAATAGATGA ACTGCGATCT CTTGATTCAC CTTCTCATAT TTCAAAAATA 
GATTTACTCA ATCTCCAGGA TCTATCAAGT GGTGCTAATT TGCTGAACAC ATCACAACAG 
CTTCCAGGTA GTGACCTACC GAGCACATGG GTTAAAGAGT TTCATACTCA GGAGCTCAGC 
AGGGAAAGTT CCTTCCACTC TAGCATTGAA GCCATCTGGG AAGAATGTAA AGAGATTGTG 
AAGGCTTCCT CCAAAAAGTC TCATCAGATT CAGGGACTAG AAGAACTAAT TGAAAAATTA 
CAGGTGGAAG TAAAAAACTG CAGGGATGAG AACAGTGAGC TTCGAGCAAA GGAGAGTGAG 
GATAAGAATC GAGACCAGCA GCTGAAGGAG AAAGAAAGTC TAATCCAGCA GCTTAGAGAA 
GAACTTCAGG AAACCACTGT CAGTCTCCGT GTCCAAGTAC AGCTAGTAGC GGAAAGAGAG 
CAAGCTCTTT CAGAGCTTTC CCGGGATGTT ACCTGCTATA AGGCAAAAGT AAAGGACCTT 
GAAGTGATGG TAGAGACCCA GAAAGAGGAA TGTAAGCGCT TAGCTGAGTT AGAGCAGAGC 
ATTTTAGAGA AGGAATCTGC CATCCTAAAG CTGGAAGCGA GTCTAAAGGA ATTGGAAGCA 
AAGCACCAGG ATCACATCAG AAGTACCACA CATTTAAATG CAGAGGAAGT CAAGTTCAGA 
GAAGAAATTA CACAGTTGGC GAATAATTTG CATGATACAA AACAGTTACT TCAGTCAAAG 
GAAGAAGAAA ATGAAATTAG CAGACAAGAG ACAGAAAAGT TGAAAGAGGA GCTTGCTGCA 
AACTCTGTTC TTACCCAGAA TCTCCAAGCA GACCTTCAGC GGAAGGAGGA AGATTGTGCT 
GAGCTAAAGG AGAAGTTCAC TGATGCCAAA AAGCAGATCG AGCAGGTATC TGTAATGCGT 
GATGAAGAGA AGTCGTTGAG GACTAAAATT AATGAACTGG AGAAAAAGAA AAACCAATAT 
TCTCAGGAAA TAGATATGAA ACAACGAACC ATTCAGCAAC TTAAGGAGCA GTTAAGTAAT 
CAGAAAATGG AAGAAGTTGT ACAACAGTAT GAGAAAGTGT GCAAAGATCT CAGCGTTAAG 
GAGAAGCTGA TTGAAGCTAT GCGACTGACA CTAGTGGAGC AGGAGCAAAC TCAGGCAGAG 
CAGGACAGGA TGCTTGAGGC TAAGTCACAG GAAGCTGACT GGCTAGCCGG AGAATTGGAT 
ACATGGAAAG ACAAATTCAA AGATCTGGAG ACTAGAAGTA ATCAAAAGGT AACTACAGAG 
GCCATGGAGG ACAGCGACGT GCTTAGTGAG AAGTTCAGGA AGCTTCAGGA CGAGCTGCAG 
GAATCTGAAG AGAAACATAA AGCTGATAGA AAGAAGTGGT TAGAAGAAAA AGCGGTTCTT 
ACAACTCAAG CAAAAGAAGC AGAGACTCTA CGAAACAGGG AGATGAAAAA ATATGCTGAG 
GACAGGGAGC GTTGTCTGAA GCTGCAGAAT GAAGTGGAAA CACTGACAGC ACAGCTGGCG 
GAGAAAACTG GTGAGCTTCA GAAGTGGAGA GAAGAGCGGG ACCAGCTGGT CACAGCTGTG 
GAGACACAGA TGCAGGCTCT GCTCTCCAGC AGCAAGCATA AGGACGAAGA GATCCAGCAA 
CTGAGAAAGG CCGTGGCAAA GAGCACGGGG ACAGTGAGTG GACGCGTACT CGAAAACCAG 
ACCATGAATC TCAAGCCTGA ATGTAATGAT TCAGTCGATC TTGGTGGAGT TGAAACTGAA 
CTTCAGTCAA CAAGTTTTGA AATTTCTAGG AATACAGCAG AGGATGGATC TGTAGTTCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATGTA CAAAGTACTC GATTTCCAAA GCCTGAACTG 
GAGATTCAGT TTACACCTCT GCAGCCAAAC AAAGTGGCAG TGAAGCACCC TGGTTGTGCC 
ACACCAGTCA CAATTAAGAT CCCCAAGGCA CGGAAGAGGA AGAGTGGCGA GGTGGAGGAG 
GACTTGGTGA AATGTGAAAA TAAGAAGAAT TCCACACCCA GAAGTAATGT GAAGTTTCCC 
GTTTCAGAGC ATAGAAATTC CTCTTTCAAA AAGGAACAAA AGGTTTTCTT ATGTCCGTCA 
TCTAAGAAGA CATACTCCTT ACGGAGCCAA GCATCTACGG TTGGTGCGAA TGTAGCCTCT 
AAAAAAAAAG AGGGAACACT ACAGAAATTT GGAGACTTCT TACAACATTC TCCAACAATC 
CTTCAATCAA AAGCAAAAAA GATAATTGAA ACAATGAGTT CCCCAAAGTT ATCAACTGTA 
GAAGTAAGTA AAGAAAATGT ATCCCAGCCT AAAAAAGCCA AACGGAAATT GTACAGAAAT 
GAAATTTCAT CTCCCATCAA CATATCTGGC CAAGTGATTT TAATGGAACA GAAAGCAAAA 
GAAACTGATC ATCAGATTCT CAAACGACGG CTTCGAACAC GAACAGCCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The vertebrate-specific Kinesin-6, Kif20b, is required for normal cytokinesis of polarized cortical stem cells and cerebral cortex size.
Development (Cambridge, England)140(23)4672-82(2013 Dec)
Janisch KM,Vock VM,Fleming MS,Shrestha A,Grimsley-Myers CM,Rasoul BA,Neale SA,Cupp TD,Kinchen JM,Liem KF Jr,Dwyer ND

Shootin1 acts in concert with KIF20B to promote polarization of migrating neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(29)11932-48(2013 Jul)
Sapir T,Levy T,Sakakibara A,Rabinkov A,Miyata T,Reiner O

Endoglin haploinsufficiency promotes fibroblast accumulation during wound healing through Akt activation.
PloS one8(1)e54687(2013)
Pericacho M,Velasco S,Prieto M,Llano E,L?pez-Novoa JM,Rodr?guez-Barbero A

Oncogenic role of MPHOSPH1, a cancer-testis antigen specific to human bladder cancer.
Cancer research67(7)3276-85(2007 Apr)
Kanehira M,Katagiri T,Shimo A,Takata R,Shuin T,Miki T,Fujioka T,Nakamura Y

Analysis of mouse embryonic patterning and morphogenesis by forward genetics.
Proceedings of the National Academy of Sciences of the United States of America102(17)5913-9(2005 Apr)
Garc?a-Garc?a MJ,Eggenschwiler JT,Caspary T,Alcorn HL,Wyler MR,Huangfu D,Rakeman AS,Lee JD,Feinberg EH,Timmer JR,Anderson KV

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Kif20b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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