kif20b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following kif20b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kif20b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa45757 XM_018095851.1
Latest version!
Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OXa61642 XM_012966777.3
Latest version!
Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa61642 XM_012966774.3
Latest version!
Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa61643 XM_004915883.4
Latest version!
Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa61644 XM_012966778.3
Latest version!
Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa12746 XM_004915883.3
Latest version!
Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa31554 XM_012966776.2
Latest version!
Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa31554 XM_012966777.2
Latest version!
Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa31554 XM_012966774.2
Latest version!
Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa31555 XM_012966778.2
Latest version!
Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa45757
    Clone ID Related Accession (Same CDS sequence) XM_018095851.1
    Accession Version XM_018095851.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF20B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGATCTTCAG
    TGGATAGAAG TGTCTGATGC AACAGAGGCA TGCAAAATTC TAGCTTTAGG GAAAAAATTC
    CAAAGCATTG CATATACAAA GCTGAACAGT TCATCTAGCA GGAGTCACAG CATCTTTACT
    GTTCGACTGT TAAAAATTGA AGATTCTGAT ATACCTCGAG TGTTGAAAGT CAGTGAATTG
    GCACTGTGTG ATTTGGCCGG TTCTGAAAGA TGTACAAAGA CTCAAAATGA AGGAGAGAGA
    CTAAAGGAAA GTGGCAATAT AAATACATCC TTGCTTATTC TGGGCAAATG TATTAATGCA
    CTGAAGACAA GTCAGCATTC AAAGGCTCAT CAGCATGTCC CATTTCGGGA AAGCAAGCTT
    ACACACTACC TACAAAGTTT TTTCAGTGGC AAAGGAAAAG TGTGCATGAT TGTTAACATC
    TGTCAGTCAG CCTCTTCTTA TGACGAGACC CTTAACGTTC TAAAATTCTC GGCTGTTGCT
    CAGAAGGTTC TTATCCTGGA GTCCTCTCAG AATTCAGAGG CTTATGGTCA GAGAAAAAGT
    GCTCGAGAGG TTTCATTCAT AATCAACAAT GCAGACAGCA AGGTATGGAA CTCAAGAAAG
    CGAGCAACAG TGCAGTGGGA TTCACGTCTT GAAGATGTGC CAGAAGATGG AAACAGTGAG
    GAGGATTCTG AGGAAGAGGA GTGCCTCGAC TGTACCTATC AAGACGAGGA GGAGGATGAC
    GGTGTTCAAG AAGATGAACT TGTTATTAAA AAAGATGCCT ACCAGAAACT ACTGGACCTT
    GTAGAAGATT TAAAAACGAA GCTGGTGAAT GAGAAAAAAG ATAAACTCCT TATGGAATTA
    AAGATCCGTG AAGAGGTAGC GAAAGAATTT ACTGAACATT TCATGCAACG AGAGAAGGAC
    TTTAGTGAAC ACTTAGAAAA AGAAAAAGAG TTGATGGAGG AACGATGTGA TGAAAGAATA
    GAAATTTTCC AAGATTTGGT GAGAAAATGT TCTAAGAGTG AGGATGAAGG AGAAGAAGAT
    GAAAATAGAG ACAATACAGC AGACCAGGTG GAACAGACCG ACACACCCAT TGATGGGCTC
    TTTATTTCCA TGCAGAATGA CCTGGCAGTA ATAAAGAAGC AGGCAATGGA TGCCCAGGTT
    CAAATTATAT CAATTCCAGA TGTTCCAGGC ATAATTTCAA AGATGGAGCA AAAGCTGAAA
    CAAATTTCTT GTGACCTTTC AAAAACACAA GAAGAACTAA AGAAAAAATC TTCTCAACTA
    GAAATCCAGA TTGCAAAATG CAGCAGAGCA ACCATTCAGT TGGAGGAAAC TGAGAAAAAA
    CTTTCTTCAA ACAAGCAGCA AATTGATCAG CTTGTGAGTA TGATCGAACA AAAGGAATCT
    GCAATCGACA GGTTAAAAGA TCTTGTCTCC CACTGGGAAG CTAAATGTGA GGACTATGAG
    AAAACTGTGA ATGGCATCAA AGGAGAGATG ATTAAGGTCA ATACCAGTGC AGCTGGCAGG
    AAGCGTCCAC CAGAGGACCA CCAGTGCCCT GAGGAACAGC CCCCTTCAAA GAAAGAATTT
    GCTGAAGTTA TTGACACACA CAGACCAGCA GCCCTTAAAC AAACACATGT CAATAACAAT
    AGCAAGGAGC CAGCCATTCA ACAAGAAGTG GAAGGTCTGC GAGTAGAAAC GGAAAATTAC
    AAAACACAAA TCCATAATCT TAACTGTAGC GTTTCTTTGT TGGAAGAGAA ACTTGCTAGA
    AGTGAAGAAC AGGTCACTGA GATGAAGTCC AAAAACCAGA CTCTGATCTC CGAACTGCAG
    GCATGTAACG ATCGTACCTT TAGCCTAGAG GGGACAGTCA AAATGCTGAT GCAGGAGTTA
    GATCAGAATA AACTGAACTC TGCCAACAAA GTGGCACAAA TCAGAACTAT CCAAAATAAA
    CTGGATGAGA TTGGCAAGAG GGTGGACATT CAAGGCAAAG CAGACGAAGA TTTTTCTAAT
    ATACATGATC TAGTCGAGGA ATGCAACCAG AAGAATTGCC AATTAGATAA TCTAAAAGAA
    AGCAATTCTG CAAAAGTCAG CTCTAACAGG GAAAGTACTT TTTACAGTGC CATTGATGGT
    TTGTGGAAAA AGTGCCAGGA AGTTCTTCAG GAGTCCAGTA AAAAAAAACA TTTGATCCAA
    GAACTTGAAC AAAAACTGGA GACATTAGAC AAAGATATAA ATACAATGAA AAATGAAAGA
    GAAGATTTAC AGACTAAGTT AAATGCCCAA ATCAGTACTC TAAATGAAAA GGATGACCTT
    ATTGTACATC TCCGTAACCT CCTGTCAGAA AGGACTGATA TTTTAGAGAA CCAAAGAAAT
    GAAGAAGCAG AGTCAGAGAA AGCGATCCAA GAGCTAAAAA ATCAAGCAAA TGCCAATGTG
    CAGCAGATAA AGGAGTTGCA ATGCCTTTTA GATTCTTATA AAGAAAAATG TGAAAGTCTG
    AAATGTTTAG AAGAACAGAA CAAAGCAAGT GCCTCTGACA CTCTCCAGCT GGAACAGAAC
    TTAAAAAAAG CACAAACAAA ATACGAGGCT TATGAAAAGG ATATAACAAC ATTAAATGAG
    GAGAACAGGA AACTAGGAAA AAATATAACA GAACTGCAAG AAAAACTTAA TGCTGCGGAA
    AAGACAAGCA AAGACAAAGA GGAGCAAGTG GGACAGTCAG CTAAAGAAAT AGAATTGTTA
    AAAAAAGACC TTTCACAGCG AGCTAGTGAG CTTAAGGTTC TCCAGCTAGA TCTCCAGAGG
    AAGGAAGAGG ACTGCACTGA GCTGAAAGAT AAACTAATGG ATTCCAAGAA ACAAATACAG
    CAGGTGGAAA AAGAGGTCTC AGGGATGCGT GAGGAAAAAC GTCTGCTAAC CAACAAAGTT
    AATGAATATG AAAAACTAAA GAACCAAATG TCTCGTGAAT TGGAAATGAA ACAACGCACA
    ATTCAACAAC TTAAGAAGGA GTCTGCTGAT AATGAGAAAA ACGGTGATGT AATGCAGTTG
    TATCAGAAAG CTTGTCAAGA GGCTCAAGAA AAAGAGAAGA TAATTGAAGA CATGAAAGAG
    ACACTTATTG AGCAAGAACA GACTCAAGTA GAACAAGATC AAGTACTTGA GGCCAAAGAA
    GTAGAAGCAG AAAAATTATC TCAAGAATTG GAGGAATGGA AACAAAAATA TAAGGAGCTT
    GAGAATAATC TCTATAAAGG ACAAGTCAAA CCTGATTGCG AAACGAATAA TGCAGAAAGA
    AATGAGGGTG AATTATCTAA GTTAAAGGAT CAGTTAAAGG AACGTGAAGA AAAGAACAAC
    ACTGAGAGAA AGAAATGGCT AGAGGAGAAA ATGAATCTTC TTGCTCAGGC TAAAGAATCC
    GAGACCCACA GGAATAAAGA GATGAGAAAA TTCGCTGAGG AGCGAGAACG CTATAACAAA
    CACCAAGCAG AGATGGAACA CCTAACATTG CAGTTGACAG AAAAAGATAA TACCTTACAG
    AAGTGGAGGG AAGAACGAGA CCAGCTTCTT TCGGCACTGG AAATTCAGCT AAAAAGTCTG
    CTTTCCAGCA ACATGGAAAA AGATAAAGAA ATTGAAAACC TTAAAAAAAG TAGTACCTCA
    AGAACTCAGG AGGAAGCTTC AGATGTGGCA GAGCTAAAGC GACTGCTTTC TGTACGAGAA
    GATGCTATTA AGGAATTACA GCAGAATCTT GCTAATTCAC AGGACTCTAA GACTAATAAA
    ACAGTAGCAA ACCAGGAGGG AACAGAAGAA AAGACAGAGA CTGTTAAACC TGAACAAGTT
    AACCGGGAGC TCAACAATAC AAAAATCTCT GTGGACAGTT CCTCCTCTGC AGATGACTGC
    CAGTCACAAG GTGGGTCAGA CACTGTATTA GATTCTTCCG CAATATCGAC TGAATTTGGC
    AAGGTCAGCA GGTTCCCAAG ACCAGAGATG GAAATTCATT TTAGCCCAAT GCGACCAAAC
    AAAATGGAAG TGAAACATCA TGGAGAAAGT TCCCCAAGAA CAGTCAAAAT CACTCGGACT
    ACAAGGAAAA GGAAGAGTGA TGAGATGGAG CAGAATATAA TGCTCTCAAG CTGCTGGAAA
    ATTAAATGTA AAGACAAAGC GGAATCTGGG GGCATTTGTC AGGAAGCTGT AAAGAGTGAA
    AATAAGAAAA ACACCATATC AAGGGCAGGT GTAAAAACTC CGTCCTCACA GTCCACCTTT
    GCCACTAACA AGAGTGAAAC AAAGAGAGTT CAGAGAAGTA CGCTGAAAAA GCAGCCGTCT
    GTTTCAAGTA CAGTGAGTAC AACTTCTGCA AGGAAAAAAG ATGGAACCCT GCAAAAGCTT
    GGCGATTTCT TGCAAAGCTC ACCTTCCATT TTCCAGACAA AAGCAAAGAA ACTACTTGAA
    ACAATTGCTG CCCCTAAAGC TCTGGATGTT GGAATCTGTA AAGCCGAGAA TGATAATAAG
    CCCAAGAAAA ATAGAAGAAA ATTATACACT ACTGACATCT CTGTGCCACT GGACATCCCT
    GCCAATTCTA TCGTTATGGA CACAAATGAG AAGGAGAGTG ATCACCTCAT CATGAAAAGG
    CGCTTAAGAA CAAGAACCGC TAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951340.1
    CDS158..5524
    Translation

    Target ORF information:

    RefSeq Version XM_018095851.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGATCTTCAG
    TGGATAGAAG TGTCTGATGC AACAGAGGCA TGCAAAATTC TAGCTTTAGG GAAAAAATTC
    CAAAGCATTG CATATACAAA GCTGAACAGT TCATCTAGCA GGAGTCACAG CATCTTTACT
    GTTCGACTGT TAAAAATTGA AGATTCTGAT ATACCTCGAG TGTTGAAAGT CAGTGAATTG
    GCACTGTGTG ATTTGGCCGG TTCTGAAAGA TGTACAAAGA CTCAAAATGA AGGAGAGAGA
    CTAAAGGAAA GTGGCAATAT AAATACATCC TTGCTTATTC TGGGCAAATG TATTAATGCA
    CTGAAGACAA GTCAGCATTC AAAGGCTCAT CAGCATGTCC CATTTCGGGA AAGCAAGCTT
    ACACACTACC TACAAAGTTT TTTCAGTGGC AAAGGAAAAG TGTGCATGAT TGTTAACATC
    TGTCAGTCAG CCTCTTCTTA TGACGAGACC CTTAACGTTC TAAAATTCTC GGCTGTTGCT
    CAGAAGGTTC TTATCCTGGA GTCCTCTCAG AATTCAGAGG CTTATGGTCA GAGAAAAAGT
    GCTCGAGAGG TTTCATTCAT AATCAACAAT GCAGACAGCA AGGTATGGAA CTCAAGAAAG
    CGAGCAACAG TGCAGTGGGA TTCACGTCTT GAAGATGTGC CAGAAGATGG AAACAGTGAG
    GAGGATTCTG AGGAAGAGGA GTGCCTCGAC TGTACCTATC AAGACGAGGA GGAGGATGAC
    GGTGTTCAAG AAGATGAACT TGTTATTAAA AAAGATGCCT ACCAGAAACT ACTGGACCTT
    GTAGAAGATT TAAAAACGAA GCTGGTGAAT GAGAAAAAAG ATAAACTCCT TATGGAATTA
    AAGATCCGTG AAGAGGTAGC GAAAGAATTT ACTGAACATT TCATGCAACG AGAGAAGGAC
    TTTAGTGAAC ACTTAGAAAA AGAAAAAGAG TTGATGGAGG AACGATGTGA TGAAAGAATA
    GAAATTTTCC AAGATTTGGT GAGAAAATGT TCTAAGAGTG AGGATGAAGG AGAAGAAGAT
    GAAAATAGAG ACAATACAGC AGACCAGGTG GAACAGACCG ACACACCCAT TGATGGGCTC
    TTTATTTCCA TGCAGAATGA CCTGGCAGTA ATAAAGAAGC AGGCAATGGA TGCCCAGGTT
    CAAATTATAT CAATTCCAGA TGTTCCAGGC ATAATTTCAA AGATGGAGCA AAAGCTGAAA
    CAAATTTCTT GTGACCTTTC AAAAACACAA GAAGAACTAA AGAAAAAATC TTCTCAACTA
    GAAATCCAGA TTGCAAAATG CAGCAGAGCA ACCATTCAGT TGGAGGAAAC TGAGAAAAAA
    CTTTCTTCAA ACAAGCAGCA AATTGATCAG CTTGTGAGTA TGATCGAACA AAAGGAATCT
    GCAATCGACA GGTTAAAAGA TCTTGTCTCC CACTGGGAAG CTAAATGTGA GGACTATGAG
    AAAACTGTGA ATGGCATCAA AGGAGAGATG ATTAAGGTCA ATACCAGTGC AGCTGGCAGG
    AAGCGTCCAC CAGAGGACCA CCAGTGCCCT GAGGAACAGC CCCCTTCAAA GAAAGAATTT
    GCTGAAGTTA TTGACACACA CAGACCAGCA GCCCTTAAAC AAACACATGT CAATAACAAT
    AGCAAGGAGC CAGCCATTCA ACAAGAAGTG GAAGGTCTGC GAGTAGAAAC GGAAAATTAC
    AAAACACAAA TCCATAATCT TAACTGTAGC GTTTCTTTGT TGGAAGAGAA ACTTGCTAGA
    AGTGAAGAAC AGGTCACTGA GATGAAGTCC AAAAACCAGA CTCTGATCTC CGAACTGCAG
    GCATGTAACG ATCGTACCTT TAGCCTAGAG GGGACAGTCA AAATGCTGAT GCAGGAGTTA
    GATCAGAATA AACTGAACTC TGCCAACAAA GTGGCACAAA TCAGAACTAT CCAAAATAAA
    CTGGATGAGA TTGGCAAGAG GGTGGACATT CAAGGCAAAG CAGACGAAGA TTTTTCTAAT
    ATACATGATC TAGTCGAGGA ATGCAACCAG AAGAATTGCC AATTAGATAA TCTAAAAGAA
    AGCAATTCTG CAAAAGTCAG CTCTAACAGG GAAAGTACTT TTTACAGTGC CATTGATGGT
    TTGTGGAAAA AGTGCCAGGA AGTTCTTCAG GAGTCCAGTA AAAAAAAACA TTTGATCCAA
    GAACTTGAAC AAAAACTGGA GACATTAGAC AAAGATATAA ATACAATGAA AAATGAAAGA
    GAAGATTTAC AGACTAAGTT AAATGCCCAA ATCAGTACTC TAAATGAAAA GGATGACCTT
    ATTGTACATC TCCGTAACCT CCTGTCAGAA AGGACTGATA TTTTAGAGAA CCAAAGAAAT
    GAAGAAGCAG AGTCAGAGAA AGCGATCCAA GAGCTAAAAA ATCAAGCAAA TGCCAATGTG
    CAGCAGATAA AGGAGTTGCA ATGCCTTTTA GATTCTTATA AAGAAAAATG TGAAAGTCTG
    AAATGTTTAG AAGAACAGAA CAAAGCAAGT GCCTCTGACA CTCTCCAGCT GGAACAGAAC
    TTAAAAAAAG CACAAACAAA ATACGAGGCT TATGAAAAGG ATATAACAAC ATTAAATGAG
    GAGAACAGGA AACTAGGAAA AAATATAACA GAACTGCAAG AAAAACTTAA TGCTGCGGAA
    AAGACAAGCA AAGACAAAGA GGAGCAAGTG GGACAGTCAG CTAAAGAAAT AGAATTGTTA
    AAAAAAGACC TTTCACAGCG AGCTAGTGAG CTTAAGGTTC TCCAGCTAGA TCTCCAGAGG
    AAGGAAGAGG ACTGCACTGA GCTGAAAGAT AAACTAATGG ATTCCAAGAA ACAAATACAG
    CAGGTGGAAA AAGAGGTCTC AGGGATGCGT GAGGAAAAAC GTCTGCTAAC CAACAAAGTT
    AATGAATATG AAAAACTAAA GAACCAAATG TCTCGTGAAT TGGAAATGAA ACAACGCACA
    ATTCAACAAC TTAAGAAGGA GTCTGCTGAT AATGAGAAAA ACGGTGATGT AATGCAGTTG
    TATCAGAAAG CTTGTCAAGA GGCTCAAGAA AAAGAGAAGA TAATTGAAGA CATGAAAGAG
    ACACTTATTG AGCAAGAACA GACTCAAGTA GAACAAGATC AAGTACTTGA GGCCAAAGAA
    GTAGAAGCAG AAAAATTATC TCAAGAATTG GAGGAATGGA AACAAAAATA TAAGGAGCTT
    GAGAATAATC TCTATAAAGG ACAAGTCAAA CCTGATTGCG AAACGAATAA TGCAGAAAGA
    AATGAGGGTG AATTATCTAA GTTAAAGGAT CAGTTAAAGG AACGTGAAGA AAAGAACAAC
    ACTGAGAGAA AGAAATGGCT AGAGGAGAAA ATGAATCTTC TTGCTCAGGC TAAAGAATCC
    GAGACCCACA GGAATAAAGA GATGAGAAAA TTCGCTGAGG AGCGAGAACG CTATAACAAA
    CACCAAGCAG AGATGGAACA CCTAACATTG CAGTTGACAG AAAAAGATAA TACCTTACAG
    AAGTGGAGGG AAGAACGAGA CCAGCTTCTT TCGGCACTGG AAATTCAGCT AAAAAGTCTG
    CTTTCCAGCA ACATGGAAAA AGATAAAGAA ATTGAAAACC TTAAAAAAAG TAGTACCTCA
    AGAACTCAGG AGGAAGCTTC AGATGTGGCA GAGCTAAAGC GACTGCTTTC TGTACGAGAA
    GATGCTATTA AGGAATTACA GCAGAATCTT GCTAATTCAC AGGACTCTAA GACTAATAAA
    ACAGTAGCAA ACCAGGAGGG AACAGAAGAA AAGACAGAGA CTGTTAAACC TGAACAAGTT
    AACCGGGAGC TCAACAATAC AAAAATCTCT GTGGACAGTT CCTCCTCTGC AGATGACTGC
    CAGTCACAAG GTGGGTCAGA CACTGTATTA GATTCTTCCG CAATATCGAC TGAATTTGGC
    AAGGTCAGCA GGTTCCCAAG ACCAGAGATG GAAATTCATT TTAGCCCAAT GCGACCAAAC
    AAAATGGAAG TGAAACATCA TGGAGAAAGT TCCCCAAGAA CAGTCAAAAT CACTCGGACT
    ACAAGGAAAA GGAAGAGTGA TGAGATGGAG CAGAATATAA TGCTCTCAAG CTGCTGGAAA
    ATTAAATGTA AAGACAAAGC GGAATCTGGG GGCATTTGTC AGGAAGCTGT AAAGAGTGAA
    AATAAGAAAA ACACCATATC AAGGGCAGGT GTAAAAACTC CGTCCTCACA GTCCACCTTT
    GCCACTAACA AGAGTGAAAC AAAGAGAGTT CAGAGAAGTA CGCTGAAAAA GCAGCCGTCT
    GTTTCAAGTA CAGTGAGTAC AACTTCTGCA AGGAAAAAAG ATGGAACCCT GCAAAAGCTT
    GGCGATTTCT TGCAAAGCTC ACCTTCCATT TTCCAGACAA AAGCAAAGAA ACTACTTGAA
    ACAATTGCTG CCCCTAAAGC TCTGGATGTT GGAATCTGTA AAGCCGAGAA TGATAATAAG
    CCCAAGAAAA ATAGAAGAAA ATTATACACT ACTGACATCT CTGTGCCACT GGACATCCCT
    GCCAATTCTA TCGTTATGGA CACAAATGAG AAGGAGAGTG ATCACCTCAT CATGAAAAGG
    CGCTTAAGAA CAAGAACCGC TAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61642
    Clone ID Related Accession (Same CDS sequence) XM_012966777.3 , XM_012966774.3
    Accession Version XM_012966777.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5595bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF20B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966777.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAGAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGGAGGGAA CAGAAGAAAA GACAGAGACT GTTAAACCTG AACAAGTTAA CCGGGAGCTC
    AACAATACAA AAATCTCTGT GGACAGTTCC TCCTCTGCAG ATGACTGCCA GTCACAAGGT
    GGGTCAGACA CTGTATTAGA TTCTTCCGCA ATATCGACTG AATTTGGCAA GGTCAGCAGG
    TTCCCAAGAC CAGAGATGGA AATTCATTTT AGCCCAATGC GACCAAACAA AATGGAAGTG
    AAACATCATG GAGAAAGTTC CCCAAGAACA GTCAAAATCA CTCGGACTAC AAGGAAAAGG
    AAGAGTGATG AGATGGAGCA GAATATAATG CTCTCAAGCT GCTGGAAAAT TAAATGTAAA
    GACAAAGCGG AATCTGGGGG CATTTGTCAG GAAGCTGTAA AGAGTGAAAA TAAGAAAAAC
    ACCATATCAA GGGCAGGTGT AAAAACTCCG TCCTCACAGT CCACCGTTGC CACTAACAAG
    AGTGAAACAA AGAGAGTTCA GAGAAGTACG CTGAAAAAGC AGCCGTCTGT TTCAAGTACA
    GTGAGTACAA CTTCTGCAAG GAAAAAAGAT GGAACCCTGC AAAAGCTTGG CGATTTCTTG
    CAAAGCTCAC CTTCCATTTT CCAGACAAAA GCAAAGAAAC TACTTGAAAC AATTGCTGCC
    CCTAAAGCTC TGGATGTTGG AATCTGTAAA GCCGAGAATG ATAATAAGCC CAAGAAAAAT
    AGAAGAAAAT TATACACTAC TGACATCTCT GTGCCACTGG ACATCCCTGC CAATTCTATC
    GTTATGGACA CAAATGAGAA GGAGAGTGAT CACCTCATCA TGAAAAGGCG CTTAAGAACA
    AGAACCGCTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822231.2
    CDS114..5708
    Translation

    Target ORF information:

    RefSeq Version XM_012966777.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966777.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAGAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGGAGGGAA CAGAAGAAAA GACAGAGACT GTTAAACCTG AACAAGTTAA CCGGGAGCTC
    AACAATACAA AAATCTCTGT GGACAGTTCC TCCTCTGCAG ATGACTGCCA GTCACAAGGT
    GGGTCAGACA CTGTATTAGA TTCTTCCGCA ATATCGACTG AATTTGGCAA GGTCAGCAGG
    TTCCCAAGAC CAGAGATGGA AATTCATTTT AGCCCAATGC GACCAAACAA AATGGAAGTG
    AAACATCATG GAGAAAGTTC CCCAAGAACA GTCAAAATCA CTCGGACTAC AAGGAAAAGG
    AAGAGTGATG AGATGGAGCA GAATATAATG CTCTCAAGCT GCTGGAAAAT TAAATGTAAA
    GACAAAGCGG AATCTGGGGG CATTTGTCAG GAAGCTGTAA AGAGTGAAAA TAAGAAAAAC
    ACCATATCAA GGGCAGGTGT AAAAACTCCG TCCTCACAGT CCACCGTTGC CACTAACAAG
    AGTGAAACAA AGAGAGTTCA GAGAAGTACG CTGAAAAAGC AGCCGTCTGT TTCAAGTACA
    GTGAGTACAA CTTCTGCAAG GAAAAAAGAT GGAACCCTGC AAAAGCTTGG CGATTTCTTG
    CAAAGCTCAC CTTCCATTTT CCAGACAAAA GCAAAGAAAC TACTTGAAAC AATTGCTGCC
    CCTAAAGCTC TGGATGTTGG AATCTGTAAA GCCGAGAATG ATAATAAGCC CAAGAAAAAT
    AGAAGAAAAT TATACACTAC TGACATCTCT GTGCCACTGG ACATCCCTGC CAATTCTATC
    GTTATGGACA CAAATGAGAA GGAGAGTGAT CACCTCATCA TGAAAAGGCG CTTAAGAACA
    AGAACCGCTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61642
    Clone ID Related Accession (Same CDS sequence) XM_012966777.3 , XM_012966774.3
    Accession Version XM_012966774.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5595bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF20B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966774.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAGAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGGAGGGAA CAGAAGAAAA GACAGAGACT GTTAAACCTG AACAAGTTAA CCGGGAGCTC
    AACAATACAA AAATCTCTGT GGACAGTTCC TCCTCTGCAG ATGACTGCCA GTCACAAGGT
    GGGTCAGACA CTGTATTAGA TTCTTCCGCA ATATCGACTG AATTTGGCAA GGTCAGCAGG
    TTCCCAAGAC CAGAGATGGA AATTCATTTT AGCCCAATGC GACCAAACAA AATGGAAGTG
    AAACATCATG GAGAAAGTTC CCCAAGAACA GTCAAAATCA CTCGGACTAC AAGGAAAAGG
    AAGAGTGATG AGATGGAGCA GAATATAATG CTCTCAAGCT GCTGGAAAAT TAAATGTAAA
    GACAAAGCGG AATCTGGGGG CATTTGTCAG GAAGCTGTAA AGAGTGAAAA TAAGAAAAAC
    ACCATATCAA GGGCAGGTGT AAAAACTCCG TCCTCACAGT CCACCGTTGC CACTAACAAG
    AGTGAAACAA AGAGAGTTCA GAGAAGTACG CTGAAAAAGC AGCCGTCTGT TTCAAGTACA
    GTGAGTACAA CTTCTGCAAG GAAAAAAGAT GGAACCCTGC AAAAGCTTGG CGATTTCTTG
    CAAAGCTCAC CTTCCATTTT CCAGACAAAA GCAAAGAAAC TACTTGAAAC AATTGCTGCC
    CCTAAAGCTC TGGATGTTGG AATCTGTAAA GCCGAGAATG ATAATAAGCC CAAGAAAAAT
    AGAAGAAAAT TATACACTAC TGACATCTCT GTGCCACTGG ACATCCCTGC CAATTCTATC
    GTTATGGACA CAAATGAGAA GGAGAGTGAT CACCTCATCA TGAAAAGGCG CTTAAGAACA
    AGAACCGCTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822228.2
    CDS172..5766
    Translation

    Target ORF information:

    RefSeq Version XM_012966774.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966774.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAGAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGGAGGGAA CAGAAGAAAA GACAGAGACT GTTAAACCTG AACAAGTTAA CCGGGAGCTC
    AACAATACAA AAATCTCTGT GGACAGTTCC TCCTCTGCAG ATGACTGCCA GTCACAAGGT
    GGGTCAGACA CTGTATTAGA TTCTTCCGCA ATATCGACTG AATTTGGCAA GGTCAGCAGG
    TTCCCAAGAC CAGAGATGGA AATTCATTTT AGCCCAATGC GACCAAACAA AATGGAAGTG
    AAACATCATG GAGAAAGTTC CCCAAGAACA GTCAAAATCA CTCGGACTAC AAGGAAAAGG
    AAGAGTGATG AGATGGAGCA GAATATAATG CTCTCAAGCT GCTGGAAAAT TAAATGTAAA
    GACAAAGCGG AATCTGGGGG CATTTGTCAG GAAGCTGTAA AGAGTGAAAA TAAGAAAAAC
    ACCATATCAA GGGCAGGTGT AAAAACTCCG TCCTCACAGT CCACCGTTGC CACTAACAAG
    AGTGAAACAA AGAGAGTTCA GAGAAGTACG CTGAAAAAGC AGCCGTCTGT TTCAAGTACA
    GTGAGTACAA CTTCTGCAAG GAAAAAAGAT GGAACCCTGC AAAAGCTTGG CGATTTCTTG
    CAAAGCTCAC CTTCCATTTT CCAGACAAAA GCAAAGAAAC TACTTGAAAC AATTGCTGCC
    CCTAAAGCTC TGGATGTTGG AATCTGTAAA GCCGAGAATG ATAATAAGCC CAAGAAAAAT
    AGAAGAAAAT TATACACTAC TGACATCTCT GTGCCACTGG ACATCCCTGC CAATTCTATC
    GTTATGGACA CAAATGAGAA GGAGAGTGAT CACCTCATCA TGAAAAGGCG CTTAAGAACA
    AGAACCGCTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61643
    Clone ID Related Accession (Same CDS sequence) XM_004915883.4
    Accession Version XM_004915883.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF20B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915883.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAGAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGGAGGGAA CAGAAGAAAA GACAGAGACT GTTAAACCTG AACAAGTTAA CCGGGAGCTC
    AACAATACAA AAATCTCTGT GGACAGTTCC TCCTCTGCAG ATGACTGCCA GTCACAAGGT
    GGGTCAGACA CTGTATTAGA TTCTTCCGCA ATATCGACTG AATTTGGCAA GGTCAGCAGG
    TTCCCAAGAC CAGAGATGGA AATTCATTTT AGCCCAATGC GACCAAACAA AATGGAAGTG
    AAACATCATG GAGAAAGTTC CCCAAGAACA GTCAAAATCA CTCGGACTAC AAGGAAAAGG
    AAGAGTGATG AGATGGAGCA GGAAGCTGTA AAGAGTGAAA ATAAGAAAAA CACCATATCA
    AGGGCAGGTG TAAAAACTCC GTCCTCACAG TCCACCGTTG CCACTAACAA GAGTGAAACA
    AAGAGAGTTC AGAGAAGTAC GCTGAAAAAG CAGCCGTCTG TTTCAAGTAC AGTGAGTACA
    ACTTCTGCAA GGAAAAAAGA TGGAACCCTG CAAAAGCTTG GCGATTTCTT GCAAAGCTCA
    CCTTCCATTT TCCAGACAAA AGCAAAGAAA CTACTTGAAA CAATTGCTGC CCCTAAAGCT
    CTGGATGTTG GAATCTGTAA AGCCGAGAAT GATAATAAGC CCAAGAAAAA TAGAAGAAAA
    TTATACACTA CTGACATCTC TGTGCCACTG GACATCCCTG CCAATTCTAT CGTTATGGAC
    ACAAATGAGA AGGAGAGTGA TCACCTCATC ATGAAAAGGC GCTTAAGAAC AAGAACCGCT
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004915940.2
    CDS171..5696
    Translation

    Target ORF information:

    RefSeq Version XM_004915883.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915883.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAGAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGGAGGGAA CAGAAGAAAA GACAGAGACT GTTAAACCTG AACAAGTTAA CCGGGAGCTC
    AACAATACAA AAATCTCTGT GGACAGTTCC TCCTCTGCAG ATGACTGCCA GTCACAAGGT
    GGGTCAGACA CTGTATTAGA TTCTTCCGCA ATATCGACTG AATTTGGCAA GGTCAGCAGG
    TTCCCAAGAC CAGAGATGGA AATTCATTTT AGCCCAATGC GACCAAACAA AATGGAAGTG
    AAACATCATG GAGAAAGTTC CCCAAGAACA GTCAAAATCA CTCGGACTAC AAGGAAAAGG
    AAGAGTGATG AGATGGAGCA GGAAGCTGTA AAGAGTGAAA ATAAGAAAAA CACCATATCA
    AGGGCAGGTG TAAAAACTCC GTCCTCACAG TCCACCGTTG CCACTAACAA GAGTGAAACA
    AAGAGAGTTC AGAGAAGTAC GCTGAAAAAG CAGCCGTCTG TTTCAAGTAC AGTGAGTACA
    ACTTCTGCAA GGAAAAAAGA TGGAACCCTG CAAAAGCTTG GCGATTTCTT GCAAAGCTCA
    CCTTCCATTT TCCAGACAAA AGCAAAGAAA CTACTTGAAA CAATTGCTGC CCCTAAAGCT
    CTGGATGTTG GAATCTGTAA AGCCGAGAAT GATAATAAGC CCAAGAAAAA TAGAAGAAAA
    TTATACACTA CTGACATCTC TGTGCCACTG GACATCCCTG CCAATTCTAT CGTTATGGAC
    ACAAATGAGA AGGAGAGTGA TCACCTCATC ATGAAAAGGC GCTTAAGAAC AAGAACCGCT
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61644
    Clone ID Related Accession (Same CDS sequence) XM_012966778.3
    Accession Version XM_012966778.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF20B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966778.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAGAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGTCACAAG GTGGGTCAGA CACTGTATTA GATTCTTCCG CAATATCGAC TGAATTTGGC
    AAGGTCAGCA GGTTCCCAAG ACCAGAGATG GAAATTCATT TTAGCCCAAT GCGACCAAAC
    AAAATGGAAG TGAAACATCA TGGAGAAAGT TCCCCAAGAA CAGTCAAAAT CACTCGGACT
    ACAAGGAAAA GGAAGAGTGA TGAGATGGAG CAGAATATAA TGCTCTCAAG CTGCTGGAAA
    ATTAAATGTA AAGACAAAGC GGAATCTGGG GGCATTTGTC AGGAAGCTGT AAAGAGTGAA
    AATAAGAAAA ACACCATATC AAGGGCAGGT GTAAAAACTC CGTCCTCACA GTCCACCGTT
    GCCACTAACA AGAGTGAAAC AAAGAGAGTT CAGAGAAGTA CGCTGAAAAA GCAGCCGTCT
    GTTTCAAGTA CAGTGAGTAC AACTTCTGCA AGGAAAAAAG ATGGAACCCT GCAAAAGCTT
    GGCGATTTCT TGCAAAGCTC ACCTTCCATT TTCCAGACAA AAGCAAAGAA ACTACTTGAA
    ACAATTGCTG CCCCTAAAGC TCTGGATGTT GGAATCTGTA AAGCCGAGAA TGATAATAAG
    CCCAAGAAAA ATAGAAGAAA ATTATACACT ACTGACATCT CTGTGCCACT GGACATCCCT
    GCCAATTCTA TCGTTATGGA CACAAATGAG AAGGAGAGTG ATCACCTCAT CATGAAAAGG
    CGCTTAAGAA CAAGAACCGC TAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822232.2
    CDS172..5658
    Translation

    Target ORF information:

    RefSeq Version XM_012966778.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966778.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAGAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGTCACAAG GTGGGTCAGA CACTGTATTA GATTCTTCCG CAATATCGAC TGAATTTGGC
    AAGGTCAGCA GGTTCCCAAG ACCAGAGATG GAAATTCATT TTAGCCCAAT GCGACCAAAC
    AAAATGGAAG TGAAACATCA TGGAGAAAGT TCCCCAAGAA CAGTCAAAAT CACTCGGACT
    ACAAGGAAAA GGAAGAGTGA TGAGATGGAG CAGAATATAA TGCTCTCAAG CTGCTGGAAA
    ATTAAATGTA AAGACAAAGC GGAATCTGGG GGCATTTGTC AGGAAGCTGT AAAGAGTGAA
    AATAAGAAAA ACACCATATC AAGGGCAGGT GTAAAAACTC CGTCCTCACA GTCCACCGTT
    GCCACTAACA AGAGTGAAAC AAAGAGAGTT CAGAGAAGTA CGCTGAAAAA GCAGCCGTCT
    GTTTCAAGTA CAGTGAGTAC AACTTCTGCA AGGAAAAAAG ATGGAACCCT GCAAAAGCTT
    GGCGATTTCT TGCAAAGCTC ACCTTCCATT TTCCAGACAA AAGCAAAGAA ACTACTTGAA
    ACAATTGCTG CCCCTAAAGC TCTGGATGTT GGAATCTGTA AAGCCGAGAA TGATAATAAG
    CCCAAGAAAA ATAGAAGAAA ATTATACACT ACTGACATCT CTGTGCCACT GGACATCCCT
    GCCAATTCTA TCGTTATGGA CACAAATGAG AAGGAGAGTG ATCACCTCAT CATGAAAAGG
    CGCTTAAGAA CAAGAACCGC TAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa12746
    Clone ID Related Accession (Same CDS sequence) XM_004915883.3
    Accession Version XM_004915883.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF20B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915883.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAAAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGGAGGGAA CAGAAGAAAA GACAGAGACT GTTAAACCTG AACAAGTTAA CCGGGAGCTC
    AACAATACAA AAATCTCTGT GGACAGTTCC TCCTCTGCAG ATGACTGCCA GTCACAAGGT
    GGGTCAGACA CTGTATTAGA TTCTTCCGCA ATATCGACTG AATTTGGCAA GGTCAGCAGG
    TTCCCAAGAC CAGAGATGGA AATTCATTTT AGCCCAATGC GACCAAACAA AATGGAAGTG
    AAACATCATG GAGAAAGTTC CCCAAGAACA GTCAAAATCA CTCGGACTAC AAGGAAAAGG
    AAGAGTGATG AGATGGAGCA GGAAGCTGTA AAGAGTGAAA ATAAGAAAAA CACCATATCA
    AGGGCAGGTG TAAAAACTCC GTCCTCACAG TCCACCTTTG CCACTAACAA GAGTGAAACA
    AAGAGAGTTC AGAGAAGTAC GCTGAAAAAG CAGCCGTCTG TTTCAAGTAC AGTGAGTACA
    ACTTCTGCAA GGAAAAAAGA TGGAACCCTG CAAAAGCTTG GCGATTTCTT GCAAAGCTCA
    CCTTCCATTT TCCAGACAAA AGCAAAGAAA CTACTTGAAA CAATTGCTGC CCCTAAAGCT
    CTGGATGTTG GAATCTGTAA AGCCGAGAAT GATAATAAGC CCAAGAAAAA TAGAAGAAAA
    TTATACACTA CTGACATCTC TGTGCCACTG GACATCCCTG CCAATTCTAT CGTTATGGAC
    ACAAATGAGA AGGAGAGTGA TCACCTCATC ATGAAAAGGC GCTTAAGAAC AAGAACCGCT
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004915940.1
    CDS158..5683
    Translation

    Target ORF information:

    RefSeq Version XM_004915883.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915883.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAAAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGGAGGGAA CAGAAGAAAA GACAGAGACT GTTAAACCTG AACAAGTTAA CCGGGAGCTC
    AACAATACAA AAATCTCTGT GGACAGTTCC TCCTCTGCAG ATGACTGCCA GTCACAAGGT
    GGGTCAGACA CTGTATTAGA TTCTTCCGCA ATATCGACTG AATTTGGCAA GGTCAGCAGG
    TTCCCAAGAC CAGAGATGGA AATTCATTTT AGCCCAATGC GACCAAACAA AATGGAAGTG
    AAACATCATG GAGAAAGTTC CCCAAGAACA GTCAAAATCA CTCGGACTAC AAGGAAAAGG
    AAGAGTGATG AGATGGAGCA GGAAGCTGTA AAGAGTGAAA ATAAGAAAAA CACCATATCA
    AGGGCAGGTG TAAAAACTCC GTCCTCACAG TCCACCTTTG CCACTAACAA GAGTGAAACA
    AAGAGAGTTC AGAGAAGTAC GCTGAAAAAG CAGCCGTCTG TTTCAAGTAC AGTGAGTACA
    ACTTCTGCAA GGAAAAAAGA TGGAACCCTG CAAAAGCTTG GCGATTTCTT GCAAAGCTCA
    CCTTCCATTT TCCAGACAAA AGCAAAGAAA CTACTTGAAA CAATTGCTGC CCCTAAAGCT
    CTGGATGTTG GAATCTGTAA AGCCGAGAAT GATAATAAGC CCAAGAAAAA TAGAAGAAAA
    TTATACACTA CTGACATCTC TGTGCCACTG GACATCCCTG CCAATTCTAT CGTTATGGAC
    ACAAATGAGA AGGAGAGTGA TCACCTCATC ATGAAAAGGC GCTTAAGAAC AAGAACCGCT
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31554
    Clone ID Related Accession (Same CDS sequence) XM_012966777.2 , XM_012966776.2 , XM_012966774.2
    Accession Version XM_012966777.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5595bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF20B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAAAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGGAGGGAA CAGAAGAAAA GACAGAGACT GTTAAACCTG AACAAGTTAA CCGGGAGCTC
    AACAATACAA AAATCTCTGT GGACAGTTCC TCCTCTGCAG ATGACTGCCA GTCACAAGGT
    GGGTCAGACA CTGTATTAGA TTCTTCCGCA ATATCGACTG AATTTGGCAA GGTCAGCAGG
    TTCCCAAGAC CAGAGATGGA AATTCATTTT AGCCCAATGC GACCAAACAA AATGGAAGTG
    AAACATCATG GAGAAAGTTC CCCAAGAACA GTCAAAATCA CTCGGACTAC AAGGAAAAGG
    AAGAGTGATG AGATGGAGCA GAATATAATG CTCTCAAGCT GCTGGAAAAT TAAATGTAAA
    GACAAAGCGG AATCTGGGGG CATTTGTCAG GAAGCTGTAA AGAGTGAAAA TAAGAAAAAC
    ACCATATCAA GGGCAGGTGT AAAAACTCCG TCCTCACAGT CCACCTTTGC CACTAACAAG
    AGTGAAACAA AGAGAGTTCA GAGAAGTACG CTGAAAAAGC AGCCGTCTGT TTCAAGTACA
    GTGAGTACAA CTTCTGCAAG GAAAAAAGAT GGAACCCTGC AAAAGCTTGG CGATTTCTTG
    CAAAGCTCAC CTTCCATTTT CCAGACAAAA GCAAAGAAAC TACTTGAAAC AATTGCTGCC
    CCTAAAGCTC TGGATGTTGG AATCTGTAAA GCCGAGAATG ATAATAAGCC CAAGAAAAAT
    AGAAGAAAAT TATACACTAC TGACATCTCT GTGCCACTGG ACATCCCTGC CAATTCTATC
    GTTATGGACA CAAATGAGAA GGAGAGTGAT CACCTCATCA TGAAAAGGCG CTTAAGAACA
    AGAACCGCTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822231.1
    CDS114..5708
    Translation

    Target ORF information:

    RefSeq Version XM_012966777.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966777.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAAAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGGAGGGAA CAGAAGAAAA GACAGAGACT GTTAAACCTG AACAAGTTAA CCGGGAGCTC
    AACAATACAA AAATCTCTGT GGACAGTTCC TCCTCTGCAG ATGACTGCCA GTCACAAGGT
    GGGTCAGACA CTGTATTAGA TTCTTCCGCA ATATCGACTG AATTTGGCAA GGTCAGCAGG
    TTCCCAAGAC CAGAGATGGA AATTCATTTT AGCCCAATGC GACCAAACAA AATGGAAGTG
    AAACATCATG GAGAAAGTTC CCCAAGAACA GTCAAAATCA CTCGGACTAC AAGGAAAAGG
    AAGAGTGATG AGATGGAGCA GAATATAATG CTCTCAAGCT GCTGGAAAAT TAAATGTAAA
    GACAAAGCGG AATCTGGGGG CATTTGTCAG GAAGCTGTAA AGAGTGAAAA TAAGAAAAAC
    ACCATATCAA GGGCAGGTGT AAAAACTCCG TCCTCACAGT CCACCTTTGC CACTAACAAG
    AGTGAAACAA AGAGAGTTCA GAGAAGTACG CTGAAAAAGC AGCCGTCTGT TTCAAGTACA
    GTGAGTACAA CTTCTGCAAG GAAAAAAGAT GGAACCCTGC AAAAGCTTGG CGATTTCTTG
    CAAAGCTCAC CTTCCATTTT CCAGACAAAA GCAAAGAAAC TACTTGAAAC AATTGCTGCC
    CCTAAAGCTC TGGATGTTGG AATCTGTAAA GCCGAGAATG ATAATAAGCC CAAGAAAAAT
    AGAAGAAAAT TATACACTAC TGACATCTCT GTGCCACTGG ACATCCCTGC CAATTCTATC
    GTTATGGACA CAAATGAGAA GGAGAGTGAT CACCTCATCA TGAAAAGGCG CTTAAGAACA
    AGAACCGCTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31554
    Clone ID Related Accession (Same CDS sequence) XM_012966777.2 , XM_012966776.2 , XM_012966774.2
    Accession Version XM_012966776.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5595bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF20B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAAAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGGAGGGAA CAGAAGAAAA GACAGAGACT GTTAAACCTG AACAAGTTAA CCGGGAGCTC
    AACAATACAA AAATCTCTGT GGACAGTTCC TCCTCTGCAG ATGACTGCCA GTCACAAGGT
    GGGTCAGACA CTGTATTAGA TTCTTCCGCA ATATCGACTG AATTTGGCAA GGTCAGCAGG
    TTCCCAAGAC CAGAGATGGA AATTCATTTT AGCCCAATGC GACCAAACAA AATGGAAGTG
    AAACATCATG GAGAAAGTTC CCCAAGAACA GTCAAAATCA CTCGGACTAC AAGGAAAAGG
    AAGAGTGATG AGATGGAGCA GAATATAATG CTCTCAAGCT GCTGGAAAAT TAAATGTAAA
    GACAAAGCGG AATCTGGGGG CATTTGTCAG GAAGCTGTAA AGAGTGAAAA TAAGAAAAAC
    ACCATATCAA GGGCAGGTGT AAAAACTCCG TCCTCACAGT CCACCTTTGC CACTAACAAG
    AGTGAAACAA AGAGAGTTCA GAGAAGTACG CTGAAAAAGC AGCCGTCTGT TTCAAGTACA
    GTGAGTACAA CTTCTGCAAG GAAAAAAGAT GGAACCCTGC AAAAGCTTGG CGATTTCTTG
    CAAAGCTCAC CTTCCATTTT CCAGACAAAA GCAAAGAAAC TACTTGAAAC AATTGCTGCC
    CCTAAAGCTC TGGATGTTGG AATCTGTAAA GCCGAGAATG ATAATAAGCC CAAGAAAAAT
    AGAAGAAAAT TATACACTAC TGACATCTCT GTGCCACTGG ACATCCCTGC CAATTCTATC
    GTTATGGACA CAAATGAGAA GGAGAGTGAT CACCTCATCA TGAAAAGGCG CTTAAGAACA
    AGAACCGCTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822230.1
    CDS185..5779
    Translation

    Target ORF information:

    RefSeq Version XM_012966776.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966776.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAAAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGGAGGGAA CAGAAGAAAA GACAGAGACT GTTAAACCTG AACAAGTTAA CCGGGAGCTC
    AACAATACAA AAATCTCTGT GGACAGTTCC TCCTCTGCAG ATGACTGCCA GTCACAAGGT
    GGGTCAGACA CTGTATTAGA TTCTTCCGCA ATATCGACTG AATTTGGCAA GGTCAGCAGG
    TTCCCAAGAC CAGAGATGGA AATTCATTTT AGCCCAATGC GACCAAACAA AATGGAAGTG
    AAACATCATG GAGAAAGTTC CCCAAGAACA GTCAAAATCA CTCGGACTAC AAGGAAAAGG
    AAGAGTGATG AGATGGAGCA GAATATAATG CTCTCAAGCT GCTGGAAAAT TAAATGTAAA
    GACAAAGCGG AATCTGGGGG CATTTGTCAG GAAGCTGTAA AGAGTGAAAA TAAGAAAAAC
    ACCATATCAA GGGCAGGTGT AAAAACTCCG TCCTCACAGT CCACCTTTGC CACTAACAAG
    AGTGAAACAA AGAGAGTTCA GAGAAGTACG CTGAAAAAGC AGCCGTCTGT TTCAAGTACA
    GTGAGTACAA CTTCTGCAAG GAAAAAAGAT GGAACCCTGC AAAAGCTTGG CGATTTCTTG
    CAAAGCTCAC CTTCCATTTT CCAGACAAAA GCAAAGAAAC TACTTGAAAC AATTGCTGCC
    CCTAAAGCTC TGGATGTTGG AATCTGTAAA GCCGAGAATG ATAATAAGCC CAAGAAAAAT
    AGAAGAAAAT TATACACTAC TGACATCTCT GTGCCACTGG ACATCCCTGC CAATTCTATC
    GTTATGGACA CAAATGAGAA GGAGAGTGAT CACCTCATCA TGAAAAGGCG CTTAAGAACA
    AGAACCGCTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31554
    Clone ID Related Accession (Same CDS sequence) XM_012966777.2 , XM_012966776.2 , XM_012966774.2
    Accession Version XM_012966774.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5595bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF20B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAAAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGGAGGGAA CAGAAGAAAA GACAGAGACT GTTAAACCTG AACAAGTTAA CCGGGAGCTC
    AACAATACAA AAATCTCTGT GGACAGTTCC TCCTCTGCAG ATGACTGCCA GTCACAAGGT
    GGGTCAGACA CTGTATTAGA TTCTTCCGCA ATATCGACTG AATTTGGCAA GGTCAGCAGG
    TTCCCAAGAC CAGAGATGGA AATTCATTTT AGCCCAATGC GACCAAACAA AATGGAAGTG
    AAACATCATG GAGAAAGTTC CCCAAGAACA GTCAAAATCA CTCGGACTAC AAGGAAAAGG
    AAGAGTGATG AGATGGAGCA GAATATAATG CTCTCAAGCT GCTGGAAAAT TAAATGTAAA
    GACAAAGCGG AATCTGGGGG CATTTGTCAG GAAGCTGTAA AGAGTGAAAA TAAGAAAAAC
    ACCATATCAA GGGCAGGTGT AAAAACTCCG TCCTCACAGT CCACCTTTGC CACTAACAAG
    AGTGAAACAA AGAGAGTTCA GAGAAGTACG CTGAAAAAGC AGCCGTCTGT TTCAAGTACA
    GTGAGTACAA CTTCTGCAAG GAAAAAAGAT GGAACCCTGC AAAAGCTTGG CGATTTCTTG
    CAAAGCTCAC CTTCCATTTT CCAGACAAAA GCAAAGAAAC TACTTGAAAC AATTGCTGCC
    CCTAAAGCTC TGGATGTTGG AATCTGTAAA GCCGAGAATG ATAATAAGCC CAAGAAAAAT
    AGAAGAAAAT TATACACTAC TGACATCTCT GTGCCACTGG ACATCCCTGC CAATTCTATC
    GTTATGGACA CAAATGAGAA GGAGAGTGAT CACCTCATCA TGAAAAGGCG CTTAAGAACA
    AGAACCGCTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822228.1
    CDS158..5752
    Translation

    Target ORF information:

    RefSeq Version XM_012966774.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966774.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAAAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGGAGGGAA CAGAAGAAAA GACAGAGACT GTTAAACCTG AACAAGTTAA CCGGGAGCTC
    AACAATACAA AAATCTCTGT GGACAGTTCC TCCTCTGCAG ATGACTGCCA GTCACAAGGT
    GGGTCAGACA CTGTATTAGA TTCTTCCGCA ATATCGACTG AATTTGGCAA GGTCAGCAGG
    TTCCCAAGAC CAGAGATGGA AATTCATTTT AGCCCAATGC GACCAAACAA AATGGAAGTG
    AAACATCATG GAGAAAGTTC CCCAAGAACA GTCAAAATCA CTCGGACTAC AAGGAAAAGG
    AAGAGTGATG AGATGGAGCA GAATATAATG CTCTCAAGCT GCTGGAAAAT TAAATGTAAA
    GACAAAGCGG AATCTGGGGG CATTTGTCAG GAAGCTGTAA AGAGTGAAAA TAAGAAAAAC
    ACCATATCAA GGGCAGGTGT AAAAACTCCG TCCTCACAGT CCACCTTTGC CACTAACAAG
    AGTGAAACAA AGAGAGTTCA GAGAAGTACG CTGAAAAAGC AGCCGTCTGT TTCAAGTACA
    GTGAGTACAA CTTCTGCAAG GAAAAAAGAT GGAACCCTGC AAAAGCTTGG CGATTTCTTG
    CAAAGCTCAC CTTCCATTTT CCAGACAAAA GCAAAGAAAC TACTTGAAAC AATTGCTGCC
    CCTAAAGCTC TGGATGTTGG AATCTGTAAA GCCGAGAATG ATAATAAGCC CAAGAAAAAT
    AGAAGAAAAT TATACACTAC TGACATCTCT GTGCCACTGG ACATCCCTGC CAATTCTATC
    GTTATGGACA CAAATGAGAA GGAGAGTGAT CACCTCATCA TGAAAAGGCG CTTAAGAACA
    AGAACCGCTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31555
    Clone ID Related Accession (Same CDS sequence) XM_012966778.2
    Accession Version XM_012966778.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF20B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAAAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGTCACAAG GTGGGTCAGA CACTGTATTA GATTCTTCCG CAATATCGAC TGAATTTGGC
    AAGGTCAGCA GGTTCCCAAG ACCAGAGATG GAAATTCATT TTAGCCCAAT GCGACCAAAC
    AAAATGGAAG TGAAACATCA TGGAGAAAGT TCCCCAAGAA CAGTCAAAAT CACTCGGACT
    ACAAGGAAAA GGAAGAGTGA TGAGATGGAG CAGAATATAA TGCTCTCAAG CTGCTGGAAA
    ATTAAATGTA AAGACAAAGC GGAATCTGGG GGCATTTGTC AGGAAGCTGT AAAGAGTGAA
    AATAAGAAAA ACACCATATC AAGGGCAGGT GTAAAAACTC CGTCCTCACA GTCCACCTTT
    GCCACTAACA AGAGTGAAAC AAAGAGAGTT CAGAGAAGTA CGCTGAAAAA GCAGCCGTCT
    GTTTCAAGTA CAGTGAGTAC AACTTCTGCA AGGAAAAAAG ATGGAACCCT GCAAAAGCTT
    GGCGATTTCT TGCAAAGCTC ACCTTCCATT TTCCAGACAA AAGCAAAGAA ACTACTTGAA
    ACAATTGCTG CCCCTAAAGC TCTGGATGTT GGAATCTGTA AAGCCGAGAA TGATAATAAG
    CCCAAGAAAA ATAGAAGAAA ATTATACACT ACTGACATCT CTGTGCCACT GGACATCCCT
    GCCAATTCTA TCGTTATGGA CACAAATGAG AAGGAGAGTG ATCACCTCAT CATGAAAAGG
    CGCTTAAGAA CAAGAACCGC TAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822232.1
    CDS158..5644
    Translation

    Target ORF information:

    RefSeq Version XM_012966778.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 20B (kif20b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966778.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGCCTG CCCAAGAAAC CTATCCGCCT CCCAGGCCTT CTTATCTCGG TGTATCAATA 
    ACTTATCCAG AAAGGGATGA ACCACAGCCG ATTGATGATC TCAGAACTAA TTTGGCTGAA
    AAGTTTTTAT CACTTGGAGG CTCAGTATCA GAAACCAACC CCAACCAGAA AAATTCTTTG
    GGGTCCCAGG AGCATATGCA GGTTTACCTT CGTGTTCGCC CCTTCACTGC CGTAGAAATT
    GAACAAAATG AAGCTCAGGA CTGCATCAGT ATTCCTGATT CCTCTAGTGT TTTGGTTAAG
    CCTCCACACA ATTCACAGTC TTGCCGACTA AGTGATAAAG GCAATGGATC TATGGCTCAG
    AAGTTTACCT TTACACATGT ATTTGGTCCG GATACAACAC AAGCACAGTT TTTTGATGGC
    ACAATAAAGC AGCATGTGAT TGATTTTATG AAGGGTCAGA ACCGTCTGAT TTTTACATAT
    GGAGTGACAA ATGCAGGCAA AACTTTCACC TTCCAAGGTA CAAAAGATGA TGCGGGTATT
    TTGCCTCGGT CAATGGATAT GCTTTTTAAT TCCATTCAAG GAAGAGTGTA TACTAAAATG
    GATGTGAAGC CATATAGATG TAAAGATTAC ATACGATTAA CCAAAGAGCA GGTTAAAGCA
    GAAGCTGCTC TTAAGAATTC TGTACTTCGT CAGATGAAAG AGGTGGATTG CAGTATACGA
    AGTAACAATA GCAGGACCTG TCAAGATACC ACAGACTTCA GTAATGATGA AGGTTTCACA
    AACTCAAGCA TGTTATCTGA TATGGACATT CATGTAAGGC AATCGGAAGA GTTCAAACTG
    CCTTTGGACA GTTTAGCTAA ATTCTCCGTC TGGGTGTCTT TTTGTGAAAT CTACAATGAG
    TGTATTTATG ATCTCCTAGA CCCAATATCG GGTGATAAGT CCTATAAAAG GAAAACGCTG
    AGGCTTGCTC TGGATCTTAA AGGGTATTCT TTTGTTAAAG ATCTTCAGTG GATAGAAGTG
    TCTGATGCAA CAGAGGCATG CAAAATTCTA GCTTTAGGGA AAAAATTCCA AAGCATTGCA
    TATACAAAGC TGAACAGTTC ATCTAGCAGG AGTCACAGCA TCTTTACTGT TCGACTGTTA
    AAAATTGAAG ATTCTGATAT ACCTCGAGTG TTGAAAGTCA GTGAATTGGC ACTGTGTGAT
    TTGGCCGGTT CTGAAAGATG TACAAAGACT CAAAATGAAG GAGAGAGACT AAAGGAAAGT
    GGCAATATAA ATACATCCTT GCTTATTCTG GGCAAATGTA TTAATGCACT GAAGACAAGT
    CAGCATTCAA AGGCTCATCA GCATGTCCCA TTTCGGGAAA GCAAGCTTAC ACACTACCTA
    CAAAGTTTTT TCAGTGGCAA AGGAAAAGTG TGCATGATTG TTAACATCTG TCAGTCAGCC
    TCTTCTTATG ACGAGACCCT TAACGTTCTA AAATTCTCGG CTGTTGCTCA GAAGGTTCTT
    ATCCTGGAGT CCTCTCAGAA TTCAGAGGCT TATGGTCAGA GAAAAAGTGC TCGAGAGGTT
    TCATTCATAA TCAACAATGC AGACAGCAAG GTATGGAACT CAAGAAAGCG AGCAACAGTG
    CAGTGGGATT CACGTCTTGA AGATGTGCCA GAAGATGGAA ACAGTGAGGA GGATTCTGAG
    GAAGAGGAGT GCCTCGACTG TACCTATCAA GACGAGGAGG AGGATGACGG TGTTCAAGAA
    GATGAACTTG TTATTAAAAA AGATGCCTAC CAGAAACTAC TGGACCTTGT AGAAGATTTA
    AAAACGAAGC TGGTGAATGA GAAAAAAGAT AAACTCCTTA TGGAATTAAA GATCCGTGAA
    GAGGTAGCGA AAGAATTTAC TGAACATTTC ATGCAACGAG AGAAGGACTT TAGTGAACAC
    TTAGAAAAAG AAAAAGAGTT GATGGAGGAA CGATGTGATG AAAGAATAGA AATTTTCCAA
    GATTTGGTGA GAAAATGTTC TAAGAGTGAG GATGAAGGAG AAGAAGATGA AAATAGAGAC
    AATACAGCAG ACCAGGTGGA ACAGACCGAC ACACCCATTG ATGGGCTCTT TATTTCCATG
    CAGAATGACC TGGCAGTAAT AAAGAAGCAG GCAATGGATG CCCAGGTTCA AATTATATCA
    ATTCCAGATG TTCCAGGCAT AATTTCAAAG ATGGAGCAAA AGCTGAAACA AATTTCTTGT
    GACCTTTCAA AAACACAAGA AGAACTAAAG AAAAAATCTT CTCAACTAGA AATCCAGATT
    GCAAAATGCA GCAGAGCAAC CATTCAGTTG GAGGAAACTG AGAAAAAACT TTCTTCAAAC
    AAGCAGCAAA TTGATCAGCT TGTGAGTATG ATCGAACAAA AGGAATCTGC AATCGACAGG
    TTAAAAGATC TTGTCTCCCA CTGGGAAGCT AAATGTGAGG ACTATGAGAA AACTGTGAAT
    GGCATCAAAG GAGAGATGAT TAAGGTCAAT ACCAGTGCAG CTGGCAGGAA GCGTCCACCA
    GAGGACCACC AGTGCCCTGA GGAACAGCCC CCTTCAAAGA AAGAATTTGC TGAAGTTATT
    GACACACACA GACCAGCAGC CCTTAAACAA ACACATGTCA ATAACAATAG CAAGGAGCCA
    GCCATTCAAC AAGAAGTGGA AGGTCTGCGA GTAGAAACGG AAAATTACAA AACACAAATC
    CATAATCTTA ACTGTAGCGT TTCTTTGTTG GAAGAGAAAC TTGCTAGAAG TGAAGAACAG
    GTCACTGAGA TGAAGTCCAA AAACCAGACT CTGATCTCCG AACTGCAGGC ATGTAACGAT
    CGTACCTTTA GCCTAGAGGG GACAGTCAAA ATGCTGATGC AGGAGTTAGA TCAGAATAAA
    CTGAACTCTG CCAACAAAGT GGCACAAATC AGAACTATCC AAAATAAACT GGATGAGATT
    GGCAAGAGGG TGGACATTCA AGGCAAAGCA GACGAAGATT TTTCTAATAT ACATGATCTA
    GTCGAGGAAT GCAACCAGAA GAATTGCCAA TTAGATAATC TAAAAGAAAG CAATTCTGCA
    AAAGTCAGCT CTAACAGGGA AAGTACTTTT TACAGTGCCA TTGATGGTTT GTGGAAAAAG
    TGCCAGGAAG TTCTTCAGGA GTCCAGTAAA AAAAAACATT TGATCCAAGA ACTTGAACAA
    AAACTGGAGA CATTAGACAA AGATATAAAT ACAATGAAAA ATGAAAGAGA AGATTTACAG
    ACTAAGTTAA ATGCCCAAAT CAGTACTCTA AATGAAAAGG ATGACCTTAT TGTACATCTC
    CGTAACCTCC TGTCAGAAAG GACTGATATT TTAGAGAACC AAAGAAATGA AGAAGCAGAG
    TCAGAGAAAG CGATCCAAGA GCTAAAAAAT CAAGCAAATG CCAATGTGCA GCAGATAAAG
    GAGTTGCAAT GCCTTTTAGA TTCTTATAAA GAAAAATGTG AAAGTCTGAA ATGTTTAGAA
    GAACAGAACA AAGCAAGTGC CTCTGACACT CTCCAGCTGG AACAGAACTT AAAAAAAGCA
    CAAACAAAAT ACGAGGCTTA TGAAAAGGAT ATAACAACAT TAAATGAGGA GAACAGGAAA
    CTAGGAAAAA ATATAACAGA ACTGCAAGAA AAACTTAATG CTGCGGAAAA GACAAGCAAA
    GACAAAGAGG AGCAAGTGGG ACAGTCAGCT AAAGAAATAG AATTGTTAAA AAAAGACCTT
    TCACAGCGAG CTAGTGAGCT TAAGGTTCTC CAGCTAGATC TCCAGAGGAA GGAAGAGGAC
    TGCACTGAGC TGAAAGATAA ACTAATGGAT TCCAAGAAAC AAATACAGCA GGTGGAAAAA
    GAGGTCTCAG GGATGCGTGA GGAAAAACGT CTGCTAACCA ACAAAGTTAA TGAATATGAA
    AAACTAAAGA ACCAAATGTC TCGTGAATTG GAAATGAAAC AACGCACAAT TCAACAACTT
    AAGAAGGAGT CTGCTGATAA TGAGAAAAAC GGTGATGTAA TGCAGTTGTA TCAGAAAGCT
    TGTCAAGAGG CTCAAGAAAA AGAGAAGATA ATTGAAGACA TGAAAGAGAC ACTTATTGAG
    CAAGAACAGA CTCAAGTAGA ACAAGATCAA GTACTTGAGG CCAAAGAAGT AGAAGCAGAA
    AAATTATCTC AAGAATTGGA GGAATGGAAA CAAAAATATA AGGAGCTTGA GAATAATCTC
    TATAAAGGAC AAGTCAAACC TGATTGCGAA ACGAATAATG CAGAAAGAAA TGAGGGTGAA
    TTATCTAAGT TAAAGGATCA GTTAAAGGAA CGTGAAGAAA AGAACAACAC TGAGAGAAAG
    AAATGGCTAG AGGAGAAAAT GAATCTTCTT GCTCAGGCTA AAGAATCCGA GACCCACAGG
    AATAAAGAGA TGAGAAAATT CGCTGAGGAG CGAGAACGCT ATAACAAACA CCAAGCAGAG
    ATGGAACACC TAACATTGCA GTTGACAGAA AAAGATAATA CCTTACAGAA GTGGAGGGAA
    GAACGAGACC AGCTTCTTTC GGCACTGGAA ATTCAGCTAA AAAGTCTGCT TTCCAGCAAC
    ATGGAAAAAG ATAAAGAAAT TGAAAACCTT AAAAAAAGTA GTACCTCAAG AACTCAGGAG
    GAAGCTTCAG ATGTGGCAGA GCTAAAGCGA CTGCTTTCTG TACGAGAAGA TGCTATTAAG
    GAATTACAGC AGAATCTTGC TAATTCACAG GACTCTAAGA CTAATAAAAC AGTAGCAAAC
    CAGTCACAAG GTGGGTCAGA CACTGTATTA GATTCTTCCG CAATATCGAC TGAATTTGGC
    AAGGTCAGCA GGTTCCCAAG ACCAGAGATG GAAATTCATT TTAGCCCAAT GCGACCAAAC
    AAAATGGAAG TGAAACATCA TGGAGAAAGT TCCCCAAGAA CAGTCAAAAT CACTCGGACT
    ACAAGGAAAA GGAAGAGTGA TGAGATGGAG CAGAATATAA TGCTCTCAAG CTGCTGGAAA
    ATTAAATGTA AAGACAAAGC GGAATCTGGG GGCATTTGTC AGGAAGCTGT AAAGAGTGAA
    AATAAGAAAA ACACCATATC AAGGGCAGGT GTAAAAACTC CGTCCTCACA GTCCACCTTT
    GCCACTAACA AGAGTGAAAC AAAGAGAGTT CAGAGAAGTA CGCTGAAAAA GCAGCCGTCT
    GTTTCAAGTA CAGTGAGTAC AACTTCTGCA AGGAAAAAAG ATGGAACCCT GCAAAAGCTT
    GGCGATTTCT TGCAAAGCTC ACCTTCCATT TTCCAGACAA AAGCAAAGAA ACTACTTGAA
    ACAATTGCTG CCCCTAAAGC TCTGGATGTT GGAATCTGTA AAGCCGAGAA TGATAATAAG
    CCCAAGAAAA ATAGAAGAAA ATTATACACT ACTGACATCT CTGTGCCACT GGACATCCCT
    GCCAATTCTA TCGTTATGGA CACAAATGAG AAGGAGAGTG ATCACCTCAT CATGAAAAGG
    CGCTTAAGAA CAAGAACCGC TAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.