kif20bb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	kif20bb
Entrez Gene ID	100007502
Full Name	kinesin family member 20Bb
Synonyms	cb152,sb:cb152
General protein information	Preferred Names kinesin family member 20Bb Names kinesin-like protein KIF20B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306554.2	XP_009304829.1	kinesin-like protein KIF20B isoform X2
XM_021480280.1	XP_021335955.1	kinesin-like protein KIF20B isoform X1
XM_009306554.3	XP_009304829.1	kinesin-like protein KIF20B isoform X4
XM_021480281.1	XP_021335956.1	kinesin-like protein KIF20B isoform X3
XM_003199582.4	XP_003199630.3	kinesin-like protein KIF20B isoform X1
XM_003199582.5	XP_003199630.3	kinesin-like protein KIF20B isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KIF20B	NP_001098728.1
Bos taurus (cattle)	LOC782598	XP_005222161.1
Mus musculus (house mouse)	Kif20b	NP_898867.1
Pan troglodytes (chimpanzee)	KIF20B	XP_507908.2
Rattus norvegicus (Norway rat)	Kif20b	NP_001101079.2
Homo sapiens (human)	KIF20B	NP_057279.2
Danio rerio (zebrafish)	kif20bb	XP_003199630.2
Canis lupus familiaris (dog)	KIF20B	XP_534957.4
Xenopus tropicalis (tropical clawed frog)	kif20b	XP_004915944.1
	LOC696152	XP_002808551.1

Gene Ontology

The following kif20bb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kif20bb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa48283	XM_021480280.1 Latest version!	Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa48285	XM_021480281.1 Latest version!	Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa48284	XM_003199582.5 Latest version!	Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa48286	XM_009306554.3 Latest version!	Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa48286	XM_009306554.2	Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa48284	XM_003199582.4	Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kif20bb ( XM_021480280.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335955.1 Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa48283
Clone ID Related Accession (Same CDS sequence)	XM_021480280.1
Accession Version	XM_021480280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF20B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAT CGTGTTTAAA TCACAAGCCT GACCGTGTCG ACTCTATAAC GGTTGAAGAC 
CTCCGAAAAG ATCTTTTCTC CGAGTTCTCC AAATTATCCA GGGACTCGGT GTCGCTTGAA 
AAAGAGCATT TGCAGGTCTA TCTCCGCGTA CGTCCGTTCA CCTCAACAGA GAGAGCTGAA 
GGAGAATCAC AGGATTGTAT CTCCATTGAG CCTCCAGATA CTGTGATTCT GAAGGCACCA 
AAAGCATCTC TGACTGCCAG ACACAGTGAG AGATTTGGAC CACAACTGGC ACAACGATTT 
CAGTTCTCAC AAGTGTACGG TCCCGAAACA ACTCAGAAAG AGATTTTTGA TGGAACAACC 
AAAAGTCTTG TTAAAGAAGT TTTGGACGGA GGGAATTCCT TGATTTTTAC ATATGGAGTT 
ACCAATGCTG GGAAGACATT TACTTTTCTT GGCCCTGAAT CAGATGGTGG AATTTTGCCC 
CGGTCATTAA ACGTGATTTT TAACAGCATA GAAGGCAGAA TTTATTTGCA AAATAACATC 
AAACCTCACC GATGTGTTGA CTTCACAAGA CTCACCAAAG AGCAGCAAGA TGAGGAAGCT 
ACAAATAAAA GAAACCTTCT GCGGCGATTT AAAGATAATG ACTCCCAGAA AACTTTGTCC 
AGCATGTCCA GCTCAAGTTG TTCATCGTTT GACAGCTCCA CATCTAGTGA CATGAATGGA 
GATAGTGTCT ATTTGGATGA GGACTCTCAT GTTAAATTCT CAGTGTGGGT CTCATTTTGT 
GAAATCTACA ATGAAAGCAT CCATGATCTG CTTGATTTTG TTCCAAATGG CTCTCACAGA 
AGGAATGCAT TGCGGTTAGC CCAAGATGTT AAAAGCAATT CTTTTGTTAA AGATCTAAAA 
TGGGTTCAGG TTAACAGTGC AGATGAAGCA TTAAAAGTTG TGAAAATTGG TAGGAAAAAT 
CAAAGTTTCT CATCCACCAA ACTCAACAAC ATCTCAAGCA GAAGCCACAG TATATTTTCC 
ATTCGGATGC TAAGGATTGA AGATGTGGGT GTTCCTCGAG TCCAGACAAT AAGCGAGTTG 
TCATTATGTG ATTTGGCTGG ATCTGAGAGA TGTGCAAGGA CTCAGAATAG AGGAGAACGC 
TTAAAAGAAG CTGGGAATAT CAACACCTCC CTGTTAACTC TAGGAAAATG CATCAATGCC 
CTGAGGCTCA ACCAAACTCA GTCCAAGTTT CATCAGCACA TCCCCTTCAG AGAGAGCAAG 
CTCACACATT ATTTGCAGGG GTATTTCTGT GGCCGTGGAA AAGCTTGCAT GATCATTAAC 
ATAAACCAGT GTGCTTCAGT GTATGATGAA ACTCTGAACG TTCTCAAGTT CTCAGCAGTG 
GCTCAGAAGG TAGTTGTCCT CAACCCTAAA CCAGCTCCAA GCATTGCACT CAAAAGATCT 
GCCAGAGATG TGTCCATGAT AATCAACAAT GCTGACAAGA AGGATTGGAC CAGGAGAAGC 
TCGATTATGG GCTGGGAGAT GAGCCTAGAG GACGTGCAGG AAGATGAAAA TGATGAAGAC 
ATGGATGAGG AAGAGGTGGA GAGTGACGAT GAGAGCATAG TGGACAGAAC TGTGCTAGAG 
ACTGTGGAAA ATCAAGAGCT GTGTGAGCTT CAGGTAAAAA TTGAAGAACT CCAGGAGAAG 
CTGATCAAGG AAGAGTCTGA AAAACTAACT TTGGAAGCCC GTATTCGTGA AGAAGTCACC 
ACAGAGTTTA TGGAGCTGTT TTCTAACATG GAAAAAGACT ACAGTGAACG TCTCCAAAGA 
GAGAAAGAAA TTGTTGAAGA AAGAGCAGAA AGGAGGCTGG AAATCCTGAA GAATCTGGTC 
AACAGGACAA TTGAGGAAAT GACTACAGAT GAAAATGCAG CAAAGGAAGC CAAGATGGAG 
CTTTTGGATA CCATGATCGA GGCAATGCGA AGCGATCTAA CTAAGATAAA GGGAGATGCA 
GAGTCTGTGC AGCTGTGTCT GGCCAATGTC CCTGAATCAC CTGGAAAACT CAGTCATCTG 
AGGACAGAAT TGGAGGAGAT AAAGGCGGAA CTTCTGAAAA GTCAACAACA ACTCAGTCTC 
AAATCTAAAG AGTTTGAAGA AAGCTGTATT CAGATGCAGG ATTTAAATGA CCAACTTCTC 
CAGGCAACCA GGAATTATGA AAACCAGAAA GTGAGATGTC AGGAACTCAT GTCTATCTGC 
CAGGAGAAAG ACGACATGGT ATCTAAATTA CAAACCGCTT TGGAACAAAA TGATGAGGCA 
GCTAGTAAGG ATAGGGCCCT AATTAACAAC ATCAGAGAAG AAATCCTACA TTTCAAAAAC 
AGCTGTAAGT GTAAGCTGAA TGAAGAGCAT GATGAAATGA CAAGTCAGAA AAATCAACAG 
CTTCAGCAAG AACTGAAAAT GAAAGATGAC CAGCTAAATC AACTTAAACT TGAGCATGCA 
TCACTTGAGA AAGCGATGCG AGATATCTCT GATAGATTGT CAGAGCTAAC TCAAACACAT 
GAAGCTACTG TAGCAATGGA GAGAGCAGAG GTCAACAAAG TCACAAATGA GAACAAGGCT 
CTTGCCAACG AGTTGGATGA ACTTCAACAG AGAGCCAATG AGATGAGCTC AAAGGTCGAA 
ACGATCCAAA CTGAATTGGG CAATCAGTCA AAGAAGGCCA ATGAGCTTTC AGAAGAACTC 
CATACTGCCA GGGCTCTGAT CAGGGAACAT CAAGAAGAGT CTTGCAGTCA CTCCCAAACT 
ATTGAATCAT TAATGATAGA GGCTGCTCAT TTGCGTCTAG AACTCAGTAC ATGCCAGCTG 
TCACGTGATA GAAAGGAAGC TGAATGTGAA AGAATGGTCG CGTTATCTCA GGAGAAAAAC 
AGACAGATCA GTGACCTGGA GCAGGAGCTC AAGCAAACCA GAGAAAGTGT GTGTCAACTA 
GAGGAACTCT GTAGTCGGCT TAAAAGTGAG CGTGATGCCC AGGCTCAAGA GTACCAGAGC 
CTTCTAAACC GTTACGAAGC ACAAAATTTA GTTGTGACTC AAATAAGAAG CAACTCTGAT 
CTTTTGGAGG AGCTGACTGC TCTAAAACAT CAGCACAAGA AAATTGAAAA ACAAGATGAT 
TGCTGGAAAA CCGTGCAGGA CAACCTCATA CAAACAGTGA GCAAACTAAA CCAGCAAGAG 
GAGATTCTGA GCATAGTGCA AACAATTGTA ACAAATGAGA TTTCTAAGGC ACGAGATGAG 
GCAAATATGA AGATAACTGA AATGGAGAAG GATCTGTCTA ACAAAAACAC AGAGCTAGAG 
ACAAAAGCCC AAGAGCTTAT GAAAGTGAGA GAACATGTGA ACGATGGCCA CAATCAAATT 
GAGCGCATGC GCTGTGATTT GCAGCAGGTG GAGCAGGAGA GATCTGATGT CAGAGATCAG 
TTATGTGAGG CTAACACTCA GAAAGAACAT TTAGAGAAAC AGATTTTAAT CTTGAGTGAA 
GAAAGCAAAA TGTTTCAGCA GAGGCTGTGT GAGGCAGAGA AGATGAAGGA TCAGCCAAGA 
CCCTGTTTGA GCTGTATAGA GCAAACTATT GATCCACTGC AGATTGAGCA GTCTAACCAT 
GTATTAGAAA AGGAAGATAT ACAGCTTTTC CAAACACCCT GTCAAGAGAG TAAGTTGATT 
GAAGAGACTC CTGTAACCTT AAACACAGAG TTTAAACGGA CAGATGAGGA GGAATTGTTG 
AAGCCCAGAC TGACTGAGAT TGAATTCATA ACTGAAGATC TTGCAACGTT GCAGGAAAAA 
AATCAAAACC CAAGAGTTGG TGTGTCTGAT CATTCTCAGC AAGAAGATGG GAGGGCCCAG 
AGACCTGAAG AACATACCAG GCAGCAGCTG GTCAAGAAGC AAGACACCAT TTTAAAACTG 
CAAAAGGATT CAGTTGTTTC TCCCAACTCT GATACCCAGG AAGCTGACAA ACTCAAACAA 
ACCTCAAACA TGAAAACAAA GAATGAAGGC GACTCTGTTA GTCCAACACA AGAGCAGGTA 
GCAGAACAGG ACTCCTCTGT ACTTTCTACA AAGAGTCTCA GGGAAGAGCG TTTTCCCAAA 
CCTGAGCTAG AAATCAGCTT CACGCCACTA AAACCTCACC GAATGAATGT CAGGAAACCG 
GGCGAAGAAG AGTCTGTCAC GATCAAACTC CGACGTACAG CCAGAAAGAG AAAGAGTACT 
GAGATAGAGA ACTCAGTGGA GTCAGAAAAC TGTAAAAACC GGAGACTGAA GGTGAACAAC 
AGGGCTAACA TGCAATCTGT TGAGACTCCC ACAGTGCAAG GAAAAAAAAT GAGTCAATTA 
AAGCAAAAAG ACTCTCCCGC TAGCCTGAAA GGTAAAAAAG ACGGACCCCT GCAGAAGATT 
GGCGACTTTA TTCAGAGCTC TCCAACCCTC TTTGGGAGCA AAGCCAAGAA GATCATGAGC 
ATGGTGAATG TTAAATCCCC CGAACCACTA AATGCATCTG AAAACAACAA GCCCAAGAGA 
TCCAAACGCA AGCTCTTCAA GACTCAAGTT TCATCTCCGA TGGACATACC ATCCTATCCT 
ATCATTGGAA GCAGTGATGA TGATAAAGAG AGCGATCATC TCATCATCAA GAGAAAACTC 
AGAACAAGAA CTGCCAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335955.1
CDS	106..4749
Translation	MMESCLNHKPDRVDSITVEDLRKDLFSEFSKLSRDSVSLEKEHLQVYLRVRPFTSTERAEGESQDCISIEPPDTVILKAPKASLTARHSERFGPQLAQRFQFSQVYGPETTQKEIFDGTTKSLVKEVLDGGNSLIFTYGVTNAGKTFTFLGPESDGGILPRSLNVIFNSIEGRIYLQNNIKPHRCVDFTRLTKEQQDEEATNKRNLLRRFKDNDSQKTLSSMSSSSCSSFDSSTSSDMNGDSVYLDEDSHVKFSVWVSFCEIYNESIHDLLDFVPNGSHRRNALRLAQDVKSNSFVKDLKWVQVNSADEALKVVKIGRKNQSFSSTKLNNISSRSHSIFSIRMLRIEDVGVPRVQTISELSLCDLAGSERCARTQNRGERLKEAGNINTSLLTLGKCINALRLNQTQSKFHQHIPFRESKLTHYLQGYFCGRGKACMIININQCASVYDETLNVLKFSAVAQKVVVLNPKPAPSIALKRSARDVSMIINNADKKDWTRRSSIMGWEMSLEDVQEDENDEDMDEEEVESDDESIVDRTVLETVENQELCELQVKIEELQEKLIKEESEKLTLEARIREEVTTEFMELFSNMEKDYSERLQREKEIVEERAERRLEILKNLVNRTIEEMTTDENAAKEAKMELLDTMIEAMRSDLTKIKGDAESVQLCLANVPESPGKLSHLRTELEEIKAELLKSQQQLSLKSKEFEESCIQMQDLNDQLLQATRNYENQKVRCQELMSICQEKDDMVSKLQTALEQNDEAASKDRALINNIREEILHFKNSCKCKLNEEHDEMTSQKNQQLQQELKMKDDQLNQLKLEHASLEKAMRDISDRLSELTQTHEATVAMERAEVNKVTNENKALANELDELQQRANEMSSKVETIQTELGNQSKKANELSEELHTARALIREHQEESCSHSQTIESLMIEAAHLRLELSTCQLSRDRKEAECERMVALSQEKNRQISDLEQELKQTRESVCQLEELCSRLKSERDAQAQEYQSLLNRYEAQNLVVTQIRSNSDLLEELTALKHQHKKIEKQDDCWKTVQDNLIQTVSKLNQQEEILSIVQTIVTNEISKARDEANMKITEMEKDLSNKNTELETKAQELMKVREHVNDGHNQIERMRCDLQQVEQERSDVRDQLCEANTQKEHLEKQILILSEESKMFQQRLCEAEKMKDQPRPCLSCIEQTIDPLQIEQSNHVLEKEDIQLFQTPCQESKLIEETPVTLNTEFKRTDEEELLKPRLTEIEFITEDLATLQEKNQNPRVGVSDHSQQEDGRAQRPEEHTRQQLVKKQDTILKLQKDSVVSPNSDTQEADKLKQTSNMKTKNEGDSVSPTQEQVAEQDSSVLSTKSLREERFPKPELEISFTPLKPHRMNVRKPGEEESVTIKLRRTARKRKSTEIENSVESENCKNRRLKVNNRANMQSVETPTVQGKKMSQLKQKDSPASLKGKKDGPLQKIGDFIQSSPTLFGSKAKKIMSMVNVKSPEPLNASENNKPKRSKRKLFKTQVSSPMDIPSYPIIGSSDDDKESDHLIIKRKLRTRTAKR

Target ORF information:

RefSeq Version	XM_021480280.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480280.1

ORF Insert Sequence:

ATGATGGAAT CGTGTTTAAA TCACAAGCCT GACCGTGTCG ACTCTATAAC GGTTGAAGAC 
CTCCGAAAAG ATCTTTTCTC CGAGTTCTCC AAATTATCCA GGGACTCGGT GTCGCTTGAA 
AAAGAGCATT TGCAGGTCTA TCTCCGCGTA CGTCCGTTCA CCTCAACAGA GAGAGCTGAA 
GGAGAATCAC AGGATTGTAT CTCCATTGAG CCTCCAGATA CTGTGATTCT GAAGGCACCA 
AAAGCATCTC TGACTGCCAG ACACAGTGAG AGATTTGGAC CACAACTGGC ACAACGATTT 
CAGTTCTCAC AAGTGTACGG TCCCGAAACA ACTCAGAAAG AGATTTTTGA TGGAACAACC 
AAAAGTCTTG TTAAAGAAGT TTTGGACGGA GGGAATTCCT TGATTTTTAC ATATGGAGTT 
ACCAATGCTG GGAAGACATT TACTTTTCTT GGCCCTGAAT CAGATGGTGG AATTTTGCCC 
CGGTCATTAA ACGTGATTTT TAACAGCATA GAAGGCAGAA TTTATTTGCA AAATAACATC 
AAACCTCACC GATGTGTTGA CTTCACAAGA CTCACCAAAG AGCAGCAAGA TGAGGAAGCT 
ACAAATAAAA GAAACCTTCT GCGGCGATTT AAAGATAATG ACTCCCAGAA AACTTTGTCC 
AGCATGTCCA GCTCAAGTTG TTCATCGTTT GACAGCTCCA CATCTAGTGA CATGAATGGA 
GATAGTGTCT ATTTGGATGA GGACTCTCAT GTTAAATTCT CAGTGTGGGT CTCATTTTGT 
GAAATCTACA ATGAAAGCAT CCATGATCTG CTTGATTTTG TTCCAAATGG CTCTCACAGA 
AGGAATGCAT TGCGGTTAGC CCAAGATGTT AAAAGCAATT CTTTTGTTAA AGATCTAAAA 
TGGGTTCAGG TTAACAGTGC AGATGAAGCA TTAAAAGTTG TGAAAATTGG TAGGAAAAAT 
CAAAGTTTCT CATCCACCAA ACTCAACAAC ATCTCAAGCA GAAGCCACAG TATATTTTCC 
ATTCGGATGC TAAGGATTGA AGATGTGGGT GTTCCTCGAG TCCAGACAAT AAGCGAGTTG 
TCATTATGTG ATTTGGCTGG ATCTGAGAGA TGTGCAAGGA CTCAGAATAG AGGAGAACGC 
TTAAAAGAAG CTGGGAATAT CAACACCTCC CTGTTAACTC TAGGAAAATG CATCAATGCC 
CTGAGGCTCA ACCAAACTCA GTCCAAGTTT CATCAGCACA TCCCCTTCAG AGAGAGCAAG 
CTCACACATT ATTTGCAGGG GTATTTCTGT GGCCGTGGAA AAGCTTGCAT GATCATTAAC 
ATAAACCAGT GTGCTTCAGT GTATGATGAA ACTCTGAACG TTCTCAAGTT CTCAGCAGTG 
GCTCAGAAGG TAGTTGTCCT CAACCCTAAA CCAGCTCCAA GCATTGCACT CAAAAGATCT 
GCCAGAGATG TGTCCATGAT AATCAACAAT GCTGACAAGA AGGATTGGAC CAGGAGAAGC 
TCGATTATGG GCTGGGAGAT GAGCCTAGAG GACGTGCAGG AAGATGAAAA TGATGAAGAC 
ATGGATGAGG AAGAGGTGGA GAGTGACGAT GAGAGCATAG TGGACAGAAC TGTGCTAGAG 
ACTGTGGAAA ATCAAGAGCT GTGTGAGCTT CAGGTAAAAA TTGAAGAACT CCAGGAGAAG 
CTGATCAAGG AAGAGTCTGA AAAACTAACT TTGGAAGCCC GTATTCGTGA AGAAGTCACC 
ACAGAGTTTA TGGAGCTGTT TTCTAACATG GAAAAAGACT ACAGTGAACG TCTCCAAAGA 
GAGAAAGAAA TTGTTGAAGA AAGAGCAGAA AGGAGGCTGG AAATCCTGAA GAATCTGGTC 
AACAGGACAA TTGAGGAAAT GACTACAGAT GAAAATGCAG CAAAGGAAGC CAAGATGGAG 
CTTTTGGATA CCATGATCGA GGCAATGCGA AGCGATCTAA CTAAGATAAA GGGAGATGCA 
GAGTCTGTGC AGCTGTGTCT GGCCAATGTC CCTGAATCAC CTGGAAAACT CAGTCATCTG 
AGGACAGAAT TGGAGGAGAT AAAGGCGGAA CTTCTGAAAA GTCAACAACA ACTCAGTCTC 
AAATCTAAAG AGTTTGAAGA AAGCTGTATT CAGATGCAGG ATTTAAATGA CCAACTTCTC 
CAGGCAACCA GGAATTATGA AAACCAGAAA GTGAGATGTC AGGAACTCAT GTCTATCTGC 
CAGGAGAAAG ACGACATGGT ATCTAAATTA CAAACCGCTT TGGAACAAAA TGATGAGGCA 
GCTAGTAAGG ATAGGGCCCT AATTAACAAC ATCAGAGAAG AAATCCTACA TTTCAAAAAC 
AGCTGTAAGT GTAAGCTGAA TGAAGAGCAT GATGAAATGA CAAGTCAGAA AAATCAACAG 
CTTCAGCAAG AACTGAAAAT GAAAGATGAC CAGCTAAATC AACTTAAACT TGAGCATGCA 
TCACTTGAGA AAGCGATGCG AGATATCTCT GATAGATTGT CAGAGCTAAC TCAAACACAT 
GAAGCTACTG TAGCAATGGA GAGAGCAGAG GTCAACAAAG TCACAAATGA GAACAAGGCT 
CTTGCCAACG AGTTGGATGA ACTTCAACAG AGAGCCAATG AGATGAGCTC AAAGGTCGAA 
ACGATCCAAA CTGAATTGGG CAATCAGTCA AAGAAGGCCA ATGAGCTTTC AGAAGAACTC 
CATACTGCCA GGGCTCTGAT CAGGGAACAT CAAGAAGAGT CTTGCAGTCA CTCCCAAACT 
ATTGAATCAT TAATGATAGA GGCTGCTCAT TTGCGTCTAG AACTCAGTAC ATGCCAGCTG 
TCACGTGATA GAAAGGAAGC TGAATGTGAA AGAATGGTCG CGTTATCTCA GGAGAAAAAC 
AGACAGATCA GTGACCTGGA GCAGGAGCTC AAGCAAACCA GAGAAAGTGT GTGTCAACTA 
GAGGAACTCT GTAGTCGGCT TAAAAGTGAG CGTGATGCCC AGGCTCAAGA GTACCAGAGC 
CTTCTAAACC GTTACGAAGC ACAAAATTTA GTTGTGACTC AAATAAGAAG CAACTCTGAT 
CTTTTGGAGG AGCTGACTGC TCTAAAACAT CAGCACAAGA AAATTGAAAA ACAAGATGAT 
TGCTGGAAAA CCGTGCAGGA CAACCTCATA CAAACAGTGA GCAAACTAAA CCAGCAAGAG 
GAGATTCTGA GCATAGTGCA AACAATTGTA ACAAATGAGA TTTCTAAGGC ACGAGATGAG 
GCAAATATGA AGATAACTGA AATGGAGAAG GATCTGTCTA ACAAAAACAC AGAGCTAGAG 
ACAAAAGCCC AAGAGCTTAT GAAAGTGAGA GAACATGTGA ACGATGGCCA CAATCAAATT 
GAGCGCATGC GCTGTGATTT GCAGCAGGTG GAGCAGGAGA GATCTGATGT CAGAGATCAG 
TTATGTGAGG CTAACACTCA GAAAGAACAT TTAGAGAAAC AGATTTTAAT CTTGAGTGAA 
GAAAGCAAAA TGTTTCAGCA GAGGCTGTGT GAGGCAGAGA AGATGAAGGA TCAGCCAAGA 
CCCTGTTTGA GCTGTATAGA GCAAACTATT GATCCACTGC AGATTGAGCA GTCTAACCAT 
GTATTAGAAA AGGAAGATAT ACAGCTTTTC CAAACACCCT GTCAAGAGAG TAAGTTGATT 
GAAGAGACTC CTGTAACCTT AAACACAGAG TTTAAACGGA CAGATGAGGA GGAATTGTTG 
AAGCCCAGAC TGACTGAGAT TGAATTCATA ACTGAAGATC TTGCAACGTT GCAGGAAAAA 
AATCAAAACC CAAGAGTTGG TGTGTCTGAT CATTCTCAGC AAGAAGATGG GAGGGCCCAG 
AGACCTGAAG AACATACCAG GCAGCAGCTG GTCAAGAAGC AAGACACCAT TTTAAAACTG 
CAAAAGGATT CAGTTGTTTC TCCCAACTCT GATACCCAGG AAGCTGACAA ACTCAAACAA 
ACCTCAAACA TGAAAACAAA GAATGAAGGC GACTCTGTTA GTCCAACACA AGAGCAGGTA 
GCAGAACAGG ACTCCTCTGT ACTTTCTACA AAGAGTCTCA GGGAAGAGCG TTTTCCCAAA 
CCTGAGCTAG AAATCAGCTT CACGCCACTA AAACCTCACC GAATGAATGT CAGGAAACCG 
GGCGAAGAAG AGTCTGTCAC GATCAAACTC CGACGTACAG CCAGAAAGAG AAAGAGTACT 
GAGATAGAGA ACTCAGTGGA GTCAGAAAAC TGTAAAAACC GGAGACTGAA GGTGAACAAC 
AGGGCTAACA TGCAATCTGT TGAGACTCCC ACAGTGCAAG GAAAAAAAAT GAGTCAATTA 
AAGCAAAAAG ACTCTCCCGC TAGCCTGAAA GGTAAAAAAG ACGGACCCCT GCAGAAGATT 
GGCGACTTTA TTCAGAGCTC TCCAACCCTC TTTGGGAGCA AAGCCAAGAA GATCATGAGC 
ATGGTGAATG TTAAATCCCC CGAACCACTA AATGCATCTG AAAACAACAA GCCCAAGAGA 
TCCAAACGCA AGCTCTTCAA GACTCAAGTT TCATCTCCGA TGGACATACC ATCCTATCCT 
ATCATTGGAA GCAGTGATGA TGATAAAGAG AGCGATCATC TCATCATCAA GAGAAAACTC 
AGAACAAGAA CTGCCAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20bb ( XM_021480281.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335956.1 Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa48285
Clone ID Related Accession (Same CDS sequence)	XM_021480281.1
Accession Version	XM_021480281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF20B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAT CGTGTTTAAA TCACAAGCCT GACCGTGTCG ACTCTATAAC GGTTGAAGAC 
CTCCGAAAAG ATCTTTTCTC CGAGTTCTCC AAATTATCCA GGGACTCGGT GTCGCTTGAA 
AAAGAGCATT TGCAGGTCTA TCTCCGCGTA CGTCCGTTCA CCTCAACAGA GAGAGCTGAA 
GGAGAATCAC AGGATTGTAT CTCCATTGAG CCTCCAGATA CTGTGATTCT GAAGGCACCA 
AAAGCATCTC TGACTGCCAG ACACAGTGAG AGATTTGGAC CACAACTGGC ACAACGATTT 
CAGTTCTCAC AAGTGTACGG TCCCGAAACA ACTCAGAAAG AGATTTTTGA TGGAACAACC 
AAAAGTCTTG TTAAAGAAGT TTTGGACGGA GGGAATTCCT TGATTTTTAC ATATGGAGTT 
ACCAATGCTG GGAAGACATT TACTTTTCTT GGCCCTGAAT CAGATGGTGG AATTTTGCCC 
CGGTCATTAA ACGTGATTTT TAACAGCATA GAAGGCAGAA TTTATTTGCA AAATAACATC 
AAACCTCACC GATGTGTTGA CTTCACAAGA CTCACCAAAG AGCAGCAAGA TGAGGAAGCT 
ACAAATAAAA GAAACCTTCT GCGGCGATTT AAAGATAATG ACTCCCAGAA AACTTTGTCC 
AGCATGTCCA GCTCAAGTTG TTCATCGTTT GACAGCTCCA CATCTAGTGA CATGAATGGA 
GATAGTGTCT ATTTGGATGA GGACTCTCAT GTTAAATTCT CAGTGTGGGT CTCATTTTGT 
GAAATCTACA ATGAAAGCAT CCATGATCTG CTTGATTTTG TTCCAAATGG CTCTCACAGA 
AGGAATGCAT TGCGGTTAGC CCAAGATGTT AAAAGCAATT CTTTTGTTAA AGATCTAAAA 
TGGGTTCAGG TTAACAGTGC AGATGAAGCA TTAAAAGTTG TGAAAATTGG TAGGAAAAAT 
CAAAGTTTCT CATCCACCAA ACTCAACAAC ATCTCAAGCA GAAGCCACAG TATATTTTCC 
ATTCGGATGC TAAGGATTGA AGATGTGGGT GTTCCTCGAG TCCAGACAAT AAGCGAGTTG 
TCATTATGTG ATTTGGCTGG ATCTGAGAGA TGTGCAAGGA CTCAGAATAG AGGAGAACGC 
TTAAAAGAAG CTGGGAATAT CAACACCTCC CTGTTAACTC TAGGAAAATG CATCAATGCC 
CTGAGGCTCA ACCAAACTCA GTCCAAGTTT CATCAGCACA TCCCCTTCAG AGAGAGCAAG 
CTCACACATT ATTTGCAGGG GTATTTCTGT GGCCGTGGAA AAGCTTGCAT GATCATTAAC 
ATAAACCAGT GTGCTTCAGT GTATGATGAA ACTCTGAACG TTCTCAAGTT CTCAGCAGTG 
GCTCAGAAGG TAGTTGTCCT CAACCCTAAA CCAGCTCCAA GCATTGCACT CAAAAGATCT 
GCCAGAGATG TGTCCATGAT AATCAACAAT GCTGACAAGA AGGATTGGAC CAGGAGAAGC 
TCGATTATGG GCTGGGAGAT GAGCCTAGAG GACGTGCAGG AAGATGAAAA TGATGAAGAC 
ATGGATGAGG AAGAGGTGGA GAGTGACGAT GAGAGCATAG TGGACAGAAC TGTGCTAGAG 
ACTGTGGAAA ATCAAGAGCT GTGTGAGCTT CAGGTAAAAA TTGAAGAACT CCAGGAGAAG 
CTGATCAAGG AAGAGTCTGA AAAACTAACT TTGGAAGCCC GTATTCGTGA AGAAGTCACC 
ACAGAGTTTA TGGAGCTGTT TTCTAACATG GAAAAAGACT ACAGTGAACG TCTCCAAAGA 
GAGAAAGAAA TTGTTGAAGA AAGAGCAGAA AGGAGGCTGG AAATCCTGAA GAATCTGGTC 
AACAGGACAA TTGAGGAAAT GACTACAGAT GAAAATGCAG CAAAGGAAGC CAAGATGGAG 
CTTTTGGATA CCATGATCGA GGCAATGCGA AGCGATCTAA CTAAGATAAA GGGAGATGCA 
GAGTCTGTGC AGCTGTGTCT GGCCAATGTC CCTGAATCAC CTGGAAAACT CAGTCATCTG 
AGGACAGAAT TGGAGGAGAT AAAGGCGGAA CTTCTGAAAA GTCAACAACA ACTCAGTCTC 
AAATCTAAAG AGTTTGAAGA AAGCTGTATT CAGATGCAGG ATTTAAATGA CCAACTTCTC 
CAGGCAACCA GGAATTATGA AAACCAGAAA GTGAGATGTC AGGAACTCAT GTCTATCTGC 
CAGGAGAAAG ACGACATGGT ATCTAAATTA CAAACCGCTT TGGAACAAAA TGATGAGGCA 
GCTAGTAAGG ATAGGGCCCT AATTAACAAC ATCAGAGAAG AAATCCTACA TTTCAAAAAC 
AGCTGTAAGT GTAAGCTGAA TGAAGAGCAT GATGAAATGA CAAGTCAGAA AAATCAACAG 
CTTCAGCAAG AACTGAAAAT GAAAGATGAC CAGCTAAATC AACTTAAACT TGAGCATGCA 
TCACTTGAGA AAGCGATGCG AGATATCTCT GATAGATTGT CAGAGCTAAC TCAAACACAT 
GAAGCTACTG TAGCAATGGA GAGAGCAGAG GTCAACAAAG TCACAAATGA GAACAAGGCT 
CTTGCCAACG AGTTGGATGA ACTTCAACAG AGAGCCAATG AGATGAGCTC AAAGGTCGAA 
ACGATCCAAA CTGAATTGGG CAATCAGTCA AAGAAGGCCA ATGAGCTTTC AGAAGAACTC 
CATACTGCCA GGGCTCTGAT CAGGGAACAT CAAGAAGAGT CTTGCAGTCA CTCCCAAACT 
ATTGAATCAT TAATGATAGA GGCTGCTCAT TTGCGTCTAG AACTCAGTAC ATGCCAGCTG 
TCACGTGATA GAAAGGAAGC TGAATGTGAA AGAATGGTCG CGTTATCTCA GGAGAAAAAC 
AGACAGATCA GTGACCTGGA GCAGGAGCTC AAGCAAACCA GAGAAAGTGT GTGTCAACTA 
GAGGAACTCT GTAGTCGGCT TAAAAGTGAG CGTGATGCCC AGGCTCAAGA GTACCAGAGC 
CTTCTAAACC GTTACGAAGC ACAAAATTTA GTTGTGACTC AAATAAGAAG CAACTCTGAT 
CTTTTGGAGG AGCTGACTGC TCTAAAACAT CAGCACAAGA AAATTGAAAA ACAAGATGAT 
TGCTGGAAAA CCGTGCAGGA CAACCTCATA CAAACAGTGA GCAAACTAAA CCAGCAAGAG 
GAGATTCTGA GCATAGTGCA AACAATTGTA ACAAATGAGA TTTCTAAGGC ACGAGATGAG 
GCAAATATGA AGATAACTGA AATGGAGAAG GATCTGTCTA ACAAAAACAC AGAGCTAGAG 
ACAAAAGCCC AAGAGCTTAT GAAAGTGAGA GAACATGTGA ACGATGGCCA CAATCAAATT 
GAGCGCATGC GCTGTGATTT GCAGCAGGTG GAGCAGGAGA GATCTGATGT CAGAGATCAG 
TTATGTGAGG CTAACACTCA GAAAGAACAT TTAGAGAAAC AGATTTTAAT CTTGAGTGAA 
GAAAGCAAAA TGTTTCAGCA GAGGCTGTGT GAGGCAGAGA AGATGAAGGA TCAGCCAAGA 
CCCTGTTTGA GCTGTATAGA GCAAACTATT GATCCACTGC AGATTGAGCA GTCTAACCAT 
GTATTAGAAA AGGAAGATAT ACAGCTTTTC CAAACACCCT GTCAAGAGAG TAAGTTGATT 
GAAGAGACTC CTGTAACCTT AAACACAGAG TTTAAACGGA CAGATGAGGA GGAATTGTTG 
AAGCCCAGAC TGACTGAGAT TGAATTCATA ACTGAAGATC TTGCAACGTT GCAGGAAAAA 
AATCAAAACC CAAGAGTTGG TGTGTCTGAT CATTCTCAGC AAGAAGATGG GAGGGCCCAG 
AGACCTGAAG AACATACCAG GCAGCAGCTG GTCAAGAAGC AAGACACCAT TTTAAAACTG 
CAAAAGGATT CAGTTGTTTC TCCCAACTCT GATACCCAGG AAGCTGACAA ACTCAAACAA 
ACCTCAAACA TGAAAACAAA GAATGAAGGC GACTCTGTTA GTCCAACACA AGAGGTAGCA 
GAACAGGACT CCTCTGTACT TTCTACAAAG AGTCTCAGGG AAGAGCGTTT TCCCAAACCT 
GAGCTAGAAA TCAGCTTCAC GCCACTAAAA CCTCACCGAA TGAATGTCAG GAAACCGGGC 
GAAGAAGAGT CTGTCACGAT CAAACTCCGA CGTACAGCCA GAAAGAGAAA GAGTACTGAG 
ATAGAGAACT CAGTGGAGTC AGAAAACTGT AAAAACCGGA GACTGAAGGT GAACAACAGG 
GCTAACATGC AATCTGTTGA GACTCCCACA GTGCAAGGAA AAAAAATGAG TCAATTAAAG 
CAAAAAGACT CTCCCGCTAG CCTGAAAGGT AAAAAAGACG GACCCCTGCA GAAGATTGGC 
GACTTTATTC AGAGCTCTCC AACCCTCTTT GGGAGCAAAG CCAAGAAGAT CATGAGCATG 
GTGAATGTTA AATCCCCCGA ACCACTAAAT GCATCTGAAA ACAACAAGCC CAAGAGATCC 
AAACGCAAGC TCTTCAAGAC TCAAGTTTCA TCTCCGATGG ACATACCATC CTATCCTATC 
ATTGGAAGCA GTGATGATGA TAAAGAGAGC GATCATCTCA TCATCAAGAG AAAACTCAGA 
ACAAGAACTG CCAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335956.1
CDS	106..4746
Translation	MMESCLNHKPDRVDSITVEDLRKDLFSEFSKLSRDSVSLEKEHLQVYLRVRPFTSTERAEGESQDCISIEPPDTVILKAPKASLTARHSERFGPQLAQRFQFSQVYGPETTQKEIFDGTTKSLVKEVLDGGNSLIFTYGVTNAGKTFTFLGPESDGGILPRSLNVIFNSIEGRIYLQNNIKPHRCVDFTRLTKEQQDEEATNKRNLLRRFKDNDSQKTLSSMSSSSCSSFDSSTSSDMNGDSVYLDEDSHVKFSVWVSFCEIYNESIHDLLDFVPNGSHRRNALRLAQDVKSNSFVKDLKWVQVNSADEALKVVKIGRKNQSFSSTKLNNISSRSHSIFSIRMLRIEDVGVPRVQTISELSLCDLAGSERCARTQNRGERLKEAGNINTSLLTLGKCINALRLNQTQSKFHQHIPFRESKLTHYLQGYFCGRGKACMIININQCASVYDETLNVLKFSAVAQKVVVLNPKPAPSIALKRSARDVSMIINNADKKDWTRRSSIMGWEMSLEDVQEDENDEDMDEEEVESDDESIVDRTVLETVENQELCELQVKIEELQEKLIKEESEKLTLEARIREEVTTEFMELFSNMEKDYSERLQREKEIVEERAERRLEILKNLVNRTIEEMTTDENAAKEAKMELLDTMIEAMRSDLTKIKGDAESVQLCLANVPESPGKLSHLRTELEEIKAELLKSQQQLSLKSKEFEESCIQMQDLNDQLLQATRNYENQKVRCQELMSICQEKDDMVSKLQTALEQNDEAASKDRALINNIREEILHFKNSCKCKLNEEHDEMTSQKNQQLQQELKMKDDQLNQLKLEHASLEKAMRDISDRLSELTQTHEATVAMERAEVNKVTNENKALANELDELQQRANEMSSKVETIQTELGNQSKKANELSEELHTARALIREHQEESCSHSQTIESLMIEAAHLRLELSTCQLSRDRKEAECERMVALSQEKNRQISDLEQELKQTRESVCQLEELCSRLKSERDAQAQEYQSLLNRYEAQNLVVTQIRSNSDLLEELTALKHQHKKIEKQDDCWKTVQDNLIQTVSKLNQQEEILSIVQTIVTNEISKARDEANMKITEMEKDLSNKNTELETKAQELMKVREHVNDGHNQIERMRCDLQQVEQERSDVRDQLCEANTQKEHLEKQILILSEESKMFQQRLCEAEKMKDQPRPCLSCIEQTIDPLQIEQSNHVLEKEDIQLFQTPCQESKLIEETPVTLNTEFKRTDEEELLKPRLTEIEFITEDLATLQEKNQNPRVGVSDHSQQEDGRAQRPEEHTRQQLVKKQDTILKLQKDSVVSPNSDTQEADKLKQTSNMKTKNEGDSVSPTQEVAEQDSSVLSTKSLREERFPKPELEISFTPLKPHRMNVRKPGEEESVTIKLRRTARKRKSTEIENSVESENCKNRRLKVNNRANMQSVETPTVQGKKMSQLKQKDSPASLKGKKDGPLQKIGDFIQSSPTLFGSKAKKIMSMVNVKSPEPLNASENNKPKRSKRKLFKTQVSSPMDIPSYPIIGSSDDDKESDHLIIKRKLRTRTAKR

Target ORF information:

RefSeq Version	XM_021480281.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480281.1

ORF Insert Sequence:

ATGATGGAAT CGTGTTTAAA TCACAAGCCT GACCGTGTCG ACTCTATAAC GGTTGAAGAC 
CTCCGAAAAG ATCTTTTCTC CGAGTTCTCC AAATTATCCA GGGACTCGGT GTCGCTTGAA 
AAAGAGCATT TGCAGGTCTA TCTCCGCGTA CGTCCGTTCA CCTCAACAGA GAGAGCTGAA 
GGAGAATCAC AGGATTGTAT CTCCATTGAG CCTCCAGATA CTGTGATTCT GAAGGCACCA 
AAAGCATCTC TGACTGCCAG ACACAGTGAG AGATTTGGAC CACAACTGGC ACAACGATTT 
CAGTTCTCAC AAGTGTACGG TCCCGAAACA ACTCAGAAAG AGATTTTTGA TGGAACAACC 
AAAAGTCTTG TTAAAGAAGT TTTGGACGGA GGGAATTCCT TGATTTTTAC ATATGGAGTT 
ACCAATGCTG GGAAGACATT TACTTTTCTT GGCCCTGAAT CAGATGGTGG AATTTTGCCC 
CGGTCATTAA ACGTGATTTT TAACAGCATA GAAGGCAGAA TTTATTTGCA AAATAACATC 
AAACCTCACC GATGTGTTGA CTTCACAAGA CTCACCAAAG AGCAGCAAGA TGAGGAAGCT 
ACAAATAAAA GAAACCTTCT GCGGCGATTT AAAGATAATG ACTCCCAGAA AACTTTGTCC 
AGCATGTCCA GCTCAAGTTG TTCATCGTTT GACAGCTCCA CATCTAGTGA CATGAATGGA 
GATAGTGTCT ATTTGGATGA GGACTCTCAT GTTAAATTCT CAGTGTGGGT CTCATTTTGT 
GAAATCTACA ATGAAAGCAT CCATGATCTG CTTGATTTTG TTCCAAATGG CTCTCACAGA 
AGGAATGCAT TGCGGTTAGC CCAAGATGTT AAAAGCAATT CTTTTGTTAA AGATCTAAAA 
TGGGTTCAGG TTAACAGTGC AGATGAAGCA TTAAAAGTTG TGAAAATTGG TAGGAAAAAT 
CAAAGTTTCT CATCCACCAA ACTCAACAAC ATCTCAAGCA GAAGCCACAG TATATTTTCC 
ATTCGGATGC TAAGGATTGA AGATGTGGGT GTTCCTCGAG TCCAGACAAT AAGCGAGTTG 
TCATTATGTG ATTTGGCTGG ATCTGAGAGA TGTGCAAGGA CTCAGAATAG AGGAGAACGC 
TTAAAAGAAG CTGGGAATAT CAACACCTCC CTGTTAACTC TAGGAAAATG CATCAATGCC 
CTGAGGCTCA ACCAAACTCA GTCCAAGTTT CATCAGCACA TCCCCTTCAG AGAGAGCAAG 
CTCACACATT ATTTGCAGGG GTATTTCTGT GGCCGTGGAA AAGCTTGCAT GATCATTAAC 
ATAAACCAGT GTGCTTCAGT GTATGATGAA ACTCTGAACG TTCTCAAGTT CTCAGCAGTG 
GCTCAGAAGG TAGTTGTCCT CAACCCTAAA CCAGCTCCAA GCATTGCACT CAAAAGATCT 
GCCAGAGATG TGTCCATGAT AATCAACAAT GCTGACAAGA AGGATTGGAC CAGGAGAAGC 
TCGATTATGG GCTGGGAGAT GAGCCTAGAG GACGTGCAGG AAGATGAAAA TGATGAAGAC 
ATGGATGAGG AAGAGGTGGA GAGTGACGAT GAGAGCATAG TGGACAGAAC TGTGCTAGAG 
ACTGTGGAAA ATCAAGAGCT GTGTGAGCTT CAGGTAAAAA TTGAAGAACT CCAGGAGAAG 
CTGATCAAGG AAGAGTCTGA AAAACTAACT TTGGAAGCCC GTATTCGTGA AGAAGTCACC 
ACAGAGTTTA TGGAGCTGTT TTCTAACATG GAAAAAGACT ACAGTGAACG TCTCCAAAGA 
GAGAAAGAAA TTGTTGAAGA AAGAGCAGAA AGGAGGCTGG AAATCCTGAA GAATCTGGTC 
AACAGGACAA TTGAGGAAAT GACTACAGAT GAAAATGCAG CAAAGGAAGC CAAGATGGAG 
CTTTTGGATA CCATGATCGA GGCAATGCGA AGCGATCTAA CTAAGATAAA GGGAGATGCA 
GAGTCTGTGC AGCTGTGTCT GGCCAATGTC CCTGAATCAC CTGGAAAACT CAGTCATCTG 
AGGACAGAAT TGGAGGAGAT AAAGGCGGAA CTTCTGAAAA GTCAACAACA ACTCAGTCTC 
AAATCTAAAG AGTTTGAAGA AAGCTGTATT CAGATGCAGG ATTTAAATGA CCAACTTCTC 
CAGGCAACCA GGAATTATGA AAACCAGAAA GTGAGATGTC AGGAACTCAT GTCTATCTGC 
CAGGAGAAAG ACGACATGGT ATCTAAATTA CAAACCGCTT TGGAACAAAA TGATGAGGCA 
GCTAGTAAGG ATAGGGCCCT AATTAACAAC ATCAGAGAAG AAATCCTACA TTTCAAAAAC 
AGCTGTAAGT GTAAGCTGAA TGAAGAGCAT GATGAAATGA CAAGTCAGAA AAATCAACAG 
CTTCAGCAAG AACTGAAAAT GAAAGATGAC CAGCTAAATC AACTTAAACT TGAGCATGCA 
TCACTTGAGA AAGCGATGCG AGATATCTCT GATAGATTGT CAGAGCTAAC TCAAACACAT 
GAAGCTACTG TAGCAATGGA GAGAGCAGAG GTCAACAAAG TCACAAATGA GAACAAGGCT 
CTTGCCAACG AGTTGGATGA ACTTCAACAG AGAGCCAATG AGATGAGCTC AAAGGTCGAA 
ACGATCCAAA CTGAATTGGG CAATCAGTCA AAGAAGGCCA ATGAGCTTTC AGAAGAACTC 
CATACTGCCA GGGCTCTGAT CAGGGAACAT CAAGAAGAGT CTTGCAGTCA CTCCCAAACT 
ATTGAATCAT TAATGATAGA GGCTGCTCAT TTGCGTCTAG AACTCAGTAC ATGCCAGCTG 
TCACGTGATA GAAAGGAAGC TGAATGTGAA AGAATGGTCG CGTTATCTCA GGAGAAAAAC 
AGACAGATCA GTGACCTGGA GCAGGAGCTC AAGCAAACCA GAGAAAGTGT GTGTCAACTA 
GAGGAACTCT GTAGTCGGCT TAAAAGTGAG CGTGATGCCC AGGCTCAAGA GTACCAGAGC 
CTTCTAAACC GTTACGAAGC ACAAAATTTA GTTGTGACTC AAATAAGAAG CAACTCTGAT 
CTTTTGGAGG AGCTGACTGC TCTAAAACAT CAGCACAAGA AAATTGAAAA ACAAGATGAT 
TGCTGGAAAA CCGTGCAGGA CAACCTCATA CAAACAGTGA GCAAACTAAA CCAGCAAGAG 
GAGATTCTGA GCATAGTGCA AACAATTGTA ACAAATGAGA TTTCTAAGGC ACGAGATGAG 
GCAAATATGA AGATAACTGA AATGGAGAAG GATCTGTCTA ACAAAAACAC AGAGCTAGAG 
ACAAAAGCCC AAGAGCTTAT GAAAGTGAGA GAACATGTGA ACGATGGCCA CAATCAAATT 
GAGCGCATGC GCTGTGATTT GCAGCAGGTG GAGCAGGAGA GATCTGATGT CAGAGATCAG 
TTATGTGAGG CTAACACTCA GAAAGAACAT TTAGAGAAAC AGATTTTAAT CTTGAGTGAA 
GAAAGCAAAA TGTTTCAGCA GAGGCTGTGT GAGGCAGAGA AGATGAAGGA TCAGCCAAGA 
CCCTGTTTGA GCTGTATAGA GCAAACTATT GATCCACTGC AGATTGAGCA GTCTAACCAT 
GTATTAGAAA AGGAAGATAT ACAGCTTTTC CAAACACCCT GTCAAGAGAG TAAGTTGATT 
GAAGAGACTC CTGTAACCTT AAACACAGAG TTTAAACGGA CAGATGAGGA GGAATTGTTG 
AAGCCCAGAC TGACTGAGAT TGAATTCATA ACTGAAGATC TTGCAACGTT GCAGGAAAAA 
AATCAAAACC CAAGAGTTGG TGTGTCTGAT CATTCTCAGC AAGAAGATGG GAGGGCCCAG 
AGACCTGAAG AACATACCAG GCAGCAGCTG GTCAAGAAGC AAGACACCAT TTTAAAACTG 
CAAAAGGATT CAGTTGTTTC TCCCAACTCT GATACCCAGG AAGCTGACAA ACTCAAACAA 
ACCTCAAACA TGAAAACAAA GAATGAAGGC GACTCTGTTA GTCCAACACA AGAGGTAGCA 
GAACAGGACT CCTCTGTACT TTCTACAAAG AGTCTCAGGG AAGAGCGTTT TCCCAAACCT 
GAGCTAGAAA TCAGCTTCAC GCCACTAAAA CCTCACCGAA TGAATGTCAG GAAACCGGGC 
GAAGAAGAGT CTGTCACGAT CAAACTCCGA CGTACAGCCA GAAAGAGAAA GAGTACTGAG 
ATAGAGAACT CAGTGGAGTC AGAAAACTGT AAAAACCGGA GACTGAAGGT GAACAACAGG 
GCTAACATGC AATCTGTTGA GACTCCCACA GTGCAAGGAA AAAAAATGAG TCAATTAAAG 
CAAAAAGACT CTCCCGCTAG CCTGAAAGGT AAAAAAGACG GACCCCTGCA GAAGATTGGC 
GACTTTATTC AGAGCTCTCC AACCCTCTTT GGGAGCAAAG CCAAGAAGAT CATGAGCATG 
GTGAATGTTA AATCCCCCGA ACCACTAAAT GCATCTGAAA ACAACAAGCC CAAGAGATCC 
AAACGCAAGC TCTTCAAGAC TCAAGTTTCA TCTCCGATGG ACATACCATC CTATCCTATC 
ATTGGAAGCA GTGATGATGA TAAAGAGAGC GATCATCTCA TCATCAAGAG AAAACTCAGA 
ACAAGAACTG CCAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20bb ( XM_003199582.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003199630.3 Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X1, mRNA.

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CloneID	ODa48284
Clone ID Related Accession (Same CDS sequence)	XM_003199582.4 , XM_003199582.5
Accession Version	XM_003199582.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF20B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003199582.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAT CGTGTTTAAA TCACAAGCCT GACCGTGTCG ACTCTATAAC GGTTGAAGAC 
CTCCGAAAAG ATCTTTTCTC CGAGTTCTCC AAATTATCCA GGGACTCGGT GTCGCTTGAA 
AAAGAGCATT TGCAGGTCTA TCTCCGCGTA CGTCCGTTCA CCTCAACAGA GAGAGCTGAA 
GGAGAATCAC AGGATTGTAT CTCCATTGAG CCTCCAGATA CTGTGATTCT GAAGGCACCA 
AAAGCATCTC TGACTGCCAG ACACAGTGAG AGATTTGGAC CACAACTGGC ACAACGATTT 
CAGTTCTCAC AAGTGTACGG TCCCGAAACA ACTCAGAAAG AGATTTTTGA TGGAACAACC 
AAAAGTCTTG TTAAAGAAGT TTTGGACGGA GGGAATTCCT TGATTTTTAC ATATGGAGTT 
ACCAATGCTG GGAAGACATT TACTTTTCTT GGCCCTGAAT CAGATGGTGG AATTTTGCCC 
CGGTCATTAA ACGTGATTTT TAACAGCATA GAAGGCAGAA TTTATTTGCA AAATAACATC 
AAACCTCACC GATGTGTTGA CTTCACAAGA CTCACCAAAG AGCAGCAAGA TGAGGAAGCT 
ACAAATAAAA GAAACCTTCT GCGGCGATTT AAAGATAATG ACTCCCAGAA AACTTTGTCC 
AGCATGTCCA GCTCAAGTTG TTCATCGTTT GACAGCTCCA CATCTAGTGA CATGAATGGA 
GATAGTGTCT ATTTGGATGA GGACTCTCAT GTTAAATTCT CAGTGTGGGT CTCATTTTGT 
GAAATCTACA ATGAAAGCAT CCATGATCTG CTTGATTTTG TTCCAAATGG CTCTCACAGA 
AGGAATGCAT TGCGGTTAGC CCAAGATGTT AAAAGCAATT CTTTTGTTAA AGATCTAAAA 
TGGGTTCAGG TTAACAGTGC AGATGAAGCA TTAAAAGTTG TGAAAATTGG TAGGAAAAAT 
CAAAGTTTCT CATCCACCAA ACTCAACAAC ATCTCAAGCA GAAGCCACAG TATATTTTCC 
ATTCGGATGC TAAGGATTGA AGATGTGGGT GTTCCTCGAG TCCAGACAAT AAGCGAGTTG 
TCATTATGTG ATTTGGCTGG ATCTGAGAGA TGTGCAAGGA CTCAGAATAG AGGAGAACGC 
TTAAAAGAAG CTGGGAATAT CAACACCTCC CTGTTAACTC TAGGAAAATG CATCAATGCC 
CTGAGGCTCA ACCAAACTCA GTCCAAGTTT CATCAGCACA TCCCCTTCAG AGAGAGCAAG 
CTCACACATT ATTTGCAGGG GTATTTCTGT GGCCGTGGAA AAGCTTGCAT GATCATTAAC 
ATAAACCAGT GTGCTTCAGT GTATGATGAA ACTCTGAACG TTCTCAAGTT CTCAGCAGTG 
GCTCAGAAGG TAGTTGTCCT CAACCCTAAA CCAGCTCCAA GCATTGCACT CAAAAGATCT 
GCCAGAGATG TGTCCATGAT AATCAACAAT GCTGACAAGA AGGATTGGAC CAGGAGAAGC 
TCGATTATGG GCTGGGAGAT GAGCCTAGAG GACGTGCAGG AAGATGAAAA TGATGAAGAC 
ATGGATGAGG AAGAGGTGGA GAGTGACGAT GAGAGCATAG TGGACAGAAC TGTGCTAGAG 
ACTGTGGAAA ATCAAGAGCT GTGTGAGCTT CAGGTAAAAA TTGAAGAACT CCAGGAGAAG 
CTGATCAAGG AAGAGTCTGA AAAACTAACT TTGGAAGCCC GTATTCGTGA AGAAGTCACC 
ACAGAGTTTA TGGAGCTGTT TTCTAACATG GAAAAAGACT ACAGTGAACG TCTCCAAAGA 
GAGAAAGAAA TTGTTGAAGA AAGAGCAGAA AGGAGGCTGG AAATCCTGAA GAATCTGGTC 
AACAGGACAA TTGAGGAAAT GACTACAGAT GAAAATGCAG CAAAGGAAGC CAAGATGGAG 
CTTTTGGATA CCATGATCGA GGCAATGCGA AGCGATCTAA CTAAGATAAA GGGAGATGCA 
GAGTCTGTGC AGCTGTGTCT GGCCAATGTC CCTGAATCAC CTGGAAAACT CAGTCATCTG 
AGGACAGAAT TGGAGGAGAT AAAGGCGGAA CTTCTGAAAA GTCAACAACA ACTCAGTCTC 
AAATCTAAAG AGTTTGAAGA AAGCTGTATT CAGATGCAGG ATTTAAATGA CCAACTTCTC 
CAGGCAACCA GGAATTATGA AAACCAGAAA GTGAGATGTC AGGAACTCAT GTCTATCTGC 
CAGGAGAAAG ACGACATGGT ATCTAAATTA CAAACCGCTT TGGAACAAAA TGATGAGGCA 
GCTAGTAAGG ATAGGGCCCT AATTAACAAC ATCAGAGAAG AAATCCTACA TTTCAAAAAC 
AGCTGTAAGT GTAAGCTGAA TGAAGAGCAT GATGAAATGA CAAGTCAGAA AAATCAACAG 
CTTCAGCAAG AACTGAAAAT GAAAGATGAC CAGCTAAATC AACTTAAACT TGAGCATGCA 
TCACTTGAGA AAGCGATGCG AGATATCTCT GATAGATTGT CAGAGCTAAC TCAAACACAT 
GAAGCTACTG TAGCAATGGA GAGAGCAGAG GTCAACAAAG TCACAAATGA GAACAAGGCT 
CTTGCCAACG AGTTGGATGA ACTTCAACAG AGAGCCAATG AGATGAGCTC AAAGGTCGAA 
ACGATCCAAA CTGAATTGGG CAATCAGTCA AAGAAGGCCA ATGAGCTTTC AGAAGAACTC 
CATACTGCCA GGGCTCTGAT CAGGGAACAT CAAGAAGAGT CTTGCAGTCA CTCCCAAACT 
ATTGAATCAT TAATGATAGA GGCTGCTCAT TTGCGTCTAG AACTCAGTAC ATGCCAGCTG 
TCACGTGATA GAAAGGAAGC TGAATGTGAA AGAATGGTCG CGTTATCTCA GGAGAAAAAC 
AGACAGATCA GTGACCTGGA GCAGGAGCTC AAGCAAACCA GAGAAAGTGT GTGTCAACTA 
GAGGAACTCT GTAGTCGGCT TAAAAGTGAG CGTGATGCCC AGGCTCAAGA GTACCAGAGC 
CTTCTAAACC GTTACGAAGC ACAAAATTTA GTTGTGACTC AAATAAGAAG CAACTCTGAT 
CTTTTGGAGG AGCTGACTGC TCTAAAACAT CAGCACAAGA AAATTGAAAA ACAAGATGAT 
TGCTGGAAAA CCGTGCAGGA CAACCTCATA CAAACAGTGA GCAAACTAAA CCAGCAAGAG 
GAGATTCTGA GCATAGTGCA AACAATTGTA ACAAATGAGA TTTCTAAGGC ACGAGATGAG 
GCAAATATGA AGATAACTGA AATGGAGAAG GATCTGTCTA ACAAAAACAC AGAGCTAGAG 
ACAAAAGCCC AAGAGCTTAT GAAAGTGAGA GAACATGTGA ACGATGGCCA CAATCAAATT 
GAGCGCATGC GCTGTGATTT GCAGCAGGTG GAGCAGGAGA GATCTGATGT CAGAGATCAG 
TTATGTGAGG CTAACACTCA GAAAGAACAT TTAGAGAAAC AGATTTTAAT CTTGAGTGAA 
GAAAGCAAAA TGTTTCAGCA GAGGCTGTGT GAGGCAGAGA AGATGAAGGA TCAGCCAAGA 
CCCTGTTTGA GCTGTATAGA GCAAACTATT GATCCACTGC AGATTGAGCA GTCTAACCAT 
GTATTAGAAA AGGAAGATAT ACAGCTTTTC CAAACACCCT GTCAAGAGAG TAAGTTGATT 
GAAGAGACTC CTGTAACCTT AAACACAGAG TTTAAACGGA CAGATGAGGA GGAATTGTTG 
AAGCCCAGAC TGACTGAGAT TGAATTCATA ACTGAAGATC TTGCAACGTT GCAGGAAAAA 
AATCAAAACC CAAGAGTTGG TGTGTCTGAT CATTCTCAGC AAGAAGATGG GAGGGCCCAG 
AGACCTGAAG AACATACCAG GCAGCTGGTC AAGAAGCAAG ACACCATTTT AAAACTGCAA 
AAGGATTCAG TTGTTTCTCC CAACTCTGAT ACCCAGGAAG CTGACAAACT CAAACAAACC 
TCAAACATGA AAACAAAGAA TGAAGGCGAC TCTGTTAGTC CAACACAAGA GCAGGTAGCA 
GAACAGGACT CCTCTGTACT TTCTACAAAG AGTCTCAGGG AAGAGCGTTT TCCCAAACCT 
GAGCTAGAAA TCAGCTTCAC GCCACTAAAA CCTCACCGAA TGAATGTCAG GAAACCGGGC 
GAAGAAGAGT CTGTCACGAT CAAACTCCGA CGTACAGCCA GAAAGAGAAA GAGTACTGAG 
ATAGAGAACT CAGTGGAGTC AGAAAACTGT AAAAACCGGA GACTGAAGGT GAACAACAGG 
GCTAACATGC AATCTGTTGA GACTCCCACA GTGCAAGGAA AAAAAATGAG TCAATTAAAG 
CAAAAAGACT CTCCCGCTAG CCTGAAAGGT AAAAAAGACG GACCCCTGCA GAAGATTGGC 
GACTTTATTC AGAGCTCTCC AACCCTCTTT GGGAGCAAAG CCAAGAAGAT CATGAGCATG 
GTGAATGTTA AATCCCCCGA ACCACTAAAT GCATCTGAAA ACAACAAGCC CAAGAGATCC 
AAACGCAAGC TCTTCAAGAC TCAAGTTTCA TCTCCGATGG ACATACCATC CTATCCTATC 
ATTGGAAGCA GTGATGATGA TAAAGAGAGC GATCATCTCA TCATCAAGAG AAAACTCAGA 
ACAAGAACTG CCAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003199630.3
CDS	109..4749
Translation	MMESCLNHKPDRVDSITVEDLRKDLFSEFSKLSRDSVSLEKEHLQVYLRVRPFTSTERAEGESQDCISIEPPDTVILKAPKASLTARHSERFGPQLAQRFQFSQVYGPETTQKEIFDGTTKSLVKEVLDGGNSLIFTYGVTNAGKTFTFLGPESDGGILPRSLNVIFNSIEGRIYLQNNIKPHRCVDFTRLTKEQQDEEATNKRNLLRRFKDNDSQKTLSSMSSSSCSSFDSSTSSDMNGDSVYLDEDSHVKFSVWVSFCEIYNESIHDLLDFVPNGSHRRNALRLAQDVKSNSFVKDLKWVQVNSADEALKVVKIGRKNQSFSSTKLNNISSRSHSIFSIRMLRIEDVGVPRVQTISELSLCDLAGSERCARTQNRGERLKEAGNINTSLLTLGKCINALRLNQTQSKFHQHIPFRESKLTHYLQGYFCGRGKACMIININQCASVYDETLNVLKFSAVAQKVVVLNPKPAPSIALKRSARDVSMIINNADKKDWTRRSSIMGWEMSLEDVQEDENDEDMDEEEVESDDESIVDRTVLETVENQELCELQVKIEELQEKLIKEESEKLTLEARIREEVTTEFMELFSNMEKDYSERLQREKEIVEERAERRLEILKNLVNRTIEEMTTDENAAKEAKMELLDTMIEAMRSDLTKIKGDAESVQLCLANVPESPGKLSHLRTELEEIKAELLKSQQQLSLKSKEFEESCIQMQDLNDQLLQATRNYENQKVRCQELMSICQEKDDMVSKLQTALEQNDEAASKDRALINNIREEILHFKNSCKCKLNEEHDEMTSQKNQQLQQELKMKDDQLNQLKLEHASLEKAMRDISDRLSELTQTHEATVAMERAEVNKVTNENKALANELDELQQRANEMSSKVETIQTELGNQSKKANELSEELHTARALIREHQEESCSHSQTIESLMIEAAHLRLELSTCQLSRDRKEAECERMVALSQEKNRQISDLEQELKQTRESVCQLEELCSRLKSERDAQAQEYQSLLNRYEAQNLVVTQIRSNSDLLEELTALKHQHKKIEKQDDCWKTVQDNLIQTVSKLNQQEEILSIVQTIVTNEISKARDEANMKITEMEKDLSNKNTELETKAQELMKVREHVNDGHNQIERMRCDLQQVEQERSDVRDQLCEANTQKEHLEKQILILSEESKMFQQRLCEAEKMKDQPRPCLSCIEQTIDPLQIEQSNHVLEKEDIQLFQTPCQESKLIEETPVTLNTEFKRTDEEELLKPRLTEIEFITEDLATLQEKNQNPRVGVSDHSQQEDGRAQRPEEHTRQLVKKQDTILKLQKDSVVSPNSDTQEADKLKQTSNMKTKNEGDSVSPTQEQVAEQDSSVLSTKSLREERFPKPELEISFTPLKPHRMNVRKPGEEESVTIKLRRTARKRKSTEIENSVESENCKNRRLKVNNRANMQSVETPTVQGKKMSQLKQKDSPASLKGKKDGPLQKIGDFIQSSPTLFGSKAKKIMSMVNVKSPEPLNASENNKPKRSKRKLFKTQVSSPMDIPSYPIIGSSDDDKESDHLIIKRKLRTRTAKR

Target ORF information:

RefSeq Version	XM_003199582.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003199582.4

ORF Insert Sequence:

ATGATGGAAT CGTGTTTAAA TCACAAGCCT GACCGTGTCG ACTCTATAAC GGTTGAAGAC 
CTCCGAAAAG ATCTTTTCTC CGAGTTCTCC AAATTATCCA GGGACTCGGT GTCGCTTGAA 
AAAGAGCATT TGCAGGTCTA TCTCCGCGTA CGTCCGTTCA CCTCAACAGA GAGAGCTGAA 
GGAGAATCAC AGGATTGTAT CTCCATTGAG CCTCCAGATA CTGTGATTCT GAAGGCACCA 
AAAGCATCTC TGACTGCCAG ACACAGTGAG AGATTTGGAC CACAACTGGC ACAACGATTT 
CAGTTCTCAC AAGTGTACGG TCCCGAAACA ACTCAGAAAG AGATTTTTGA TGGAACAACC 
AAAAGTCTTG TTAAAGAAGT TTTGGACGGA GGGAATTCCT TGATTTTTAC ATATGGAGTT 
ACCAATGCTG GGAAGACATT TACTTTTCTT GGCCCTGAAT CAGATGGTGG AATTTTGCCC 
CGGTCATTAA ACGTGATTTT TAACAGCATA GAAGGCAGAA TTTATTTGCA AAATAACATC 
AAACCTCACC GATGTGTTGA CTTCACAAGA CTCACCAAAG AGCAGCAAGA TGAGGAAGCT 
ACAAATAAAA GAAACCTTCT GCGGCGATTT AAAGATAATG ACTCCCAGAA AACTTTGTCC 
AGCATGTCCA GCTCAAGTTG TTCATCGTTT GACAGCTCCA CATCTAGTGA CATGAATGGA 
GATAGTGTCT ATTTGGATGA GGACTCTCAT GTTAAATTCT CAGTGTGGGT CTCATTTTGT 
GAAATCTACA ATGAAAGCAT CCATGATCTG CTTGATTTTG TTCCAAATGG CTCTCACAGA 
AGGAATGCAT TGCGGTTAGC CCAAGATGTT AAAAGCAATT CTTTTGTTAA AGATCTAAAA 
TGGGTTCAGG TTAACAGTGC AGATGAAGCA TTAAAAGTTG TGAAAATTGG TAGGAAAAAT 
CAAAGTTTCT CATCCACCAA ACTCAACAAC ATCTCAAGCA GAAGCCACAG TATATTTTCC 
ATTCGGATGC TAAGGATTGA AGATGTGGGT GTTCCTCGAG TCCAGACAAT AAGCGAGTTG 
TCATTATGTG ATTTGGCTGG ATCTGAGAGA TGTGCAAGGA CTCAGAATAG AGGAGAACGC 
TTAAAAGAAG CTGGGAATAT CAACACCTCC CTGTTAACTC TAGGAAAATG CATCAATGCC 
CTGAGGCTCA ACCAAACTCA GTCCAAGTTT CATCAGCACA TCCCCTTCAG AGAGAGCAAG 
CTCACACATT ATTTGCAGGG GTATTTCTGT GGCCGTGGAA AAGCTTGCAT GATCATTAAC 
ATAAACCAGT GTGCTTCAGT GTATGATGAA ACTCTGAACG TTCTCAAGTT CTCAGCAGTG 
GCTCAGAAGG TAGTTGTCCT CAACCCTAAA CCAGCTCCAA GCATTGCACT CAAAAGATCT 
GCCAGAGATG TGTCCATGAT AATCAACAAT GCTGACAAGA AGGATTGGAC CAGGAGAAGC 
TCGATTATGG GCTGGGAGAT GAGCCTAGAG GACGTGCAGG AAGATGAAAA TGATGAAGAC 
ATGGATGAGG AAGAGGTGGA GAGTGACGAT GAGAGCATAG TGGACAGAAC TGTGCTAGAG 
ACTGTGGAAA ATCAAGAGCT GTGTGAGCTT CAGGTAAAAA TTGAAGAACT CCAGGAGAAG 
CTGATCAAGG AAGAGTCTGA AAAACTAACT TTGGAAGCCC GTATTCGTGA AGAAGTCACC 
ACAGAGTTTA TGGAGCTGTT TTCTAACATG GAAAAAGACT ACAGTGAACG TCTCCAAAGA 
GAGAAAGAAA TTGTTGAAGA AAGAGCAGAA AGGAGGCTGG AAATCCTGAA GAATCTGGTC 
AACAGGACAA TTGAGGAAAT GACTACAGAT GAAAATGCAG CAAAGGAAGC CAAGATGGAG 
CTTTTGGATA CCATGATCGA GGCAATGCGA AGCGATCTAA CTAAGATAAA GGGAGATGCA 
GAGTCTGTGC AGCTGTGTCT GGCCAATGTC CCTGAATCAC CTGGAAAACT CAGTCATCTG 
AGGACAGAAT TGGAGGAGAT AAAGGCGGAA CTTCTGAAAA GTCAACAACA ACTCAGTCTC 
AAATCTAAAG AGTTTGAAGA AAGCTGTATT CAGATGCAGG ATTTAAATGA CCAACTTCTC 
CAGGCAACCA GGAATTATGA AAACCAGAAA GTGAGATGTC AGGAACTCAT GTCTATCTGC 
CAGGAGAAAG ACGACATGGT ATCTAAATTA CAAACCGCTT TGGAACAAAA TGATGAGGCA 
GCTAGTAAGG ATAGGGCCCT AATTAACAAC ATCAGAGAAG AAATCCTACA TTTCAAAAAC 
AGCTGTAAGT GTAAGCTGAA TGAAGAGCAT GATGAAATGA CAAGTCAGAA AAATCAACAG 
CTTCAGCAAG AACTGAAAAT GAAAGATGAC CAGCTAAATC AACTTAAACT TGAGCATGCA 
TCACTTGAGA AAGCGATGCG AGATATCTCT GATAGATTGT CAGAGCTAAC TCAAACACAT 
GAAGCTACTG TAGCAATGGA GAGAGCAGAG GTCAACAAAG TCACAAATGA GAACAAGGCT 
CTTGCCAACG AGTTGGATGA ACTTCAACAG AGAGCCAATG AGATGAGCTC AAAGGTCGAA 
ACGATCCAAA CTGAATTGGG CAATCAGTCA AAGAAGGCCA ATGAGCTTTC AGAAGAACTC 
CATACTGCCA GGGCTCTGAT CAGGGAACAT CAAGAAGAGT CTTGCAGTCA CTCCCAAACT 
ATTGAATCAT TAATGATAGA GGCTGCTCAT TTGCGTCTAG AACTCAGTAC ATGCCAGCTG 
TCACGTGATA GAAAGGAAGC TGAATGTGAA AGAATGGTCG CGTTATCTCA GGAGAAAAAC 
AGACAGATCA GTGACCTGGA GCAGGAGCTC AAGCAAACCA GAGAAAGTGT GTGTCAACTA 
GAGGAACTCT GTAGTCGGCT TAAAAGTGAG CGTGATGCCC AGGCTCAAGA GTACCAGAGC 
CTTCTAAACC GTTACGAAGC ACAAAATTTA GTTGTGACTC AAATAAGAAG CAACTCTGAT 
CTTTTGGAGG AGCTGACTGC TCTAAAACAT CAGCACAAGA AAATTGAAAA ACAAGATGAT 
TGCTGGAAAA CCGTGCAGGA CAACCTCATA CAAACAGTGA GCAAACTAAA CCAGCAAGAG 
GAGATTCTGA GCATAGTGCA AACAATTGTA ACAAATGAGA TTTCTAAGGC ACGAGATGAG 
GCAAATATGA AGATAACTGA AATGGAGAAG GATCTGTCTA ACAAAAACAC AGAGCTAGAG 
ACAAAAGCCC AAGAGCTTAT GAAAGTGAGA GAACATGTGA ACGATGGCCA CAATCAAATT 
GAGCGCATGC GCTGTGATTT GCAGCAGGTG GAGCAGGAGA GATCTGATGT CAGAGATCAG 
TTATGTGAGG CTAACACTCA GAAAGAACAT TTAGAGAAAC AGATTTTAAT CTTGAGTGAA 
GAAAGCAAAA TGTTTCAGCA GAGGCTGTGT GAGGCAGAGA AGATGAAGGA TCAGCCAAGA 
CCCTGTTTGA GCTGTATAGA GCAAACTATT GATCCACTGC AGATTGAGCA GTCTAACCAT 
GTATTAGAAA AGGAAGATAT ACAGCTTTTC CAAACACCCT GTCAAGAGAG TAAGTTGATT 
GAAGAGACTC CTGTAACCTT AAACACAGAG TTTAAACGGA CAGATGAGGA GGAATTGTTG 
AAGCCCAGAC TGACTGAGAT TGAATTCATA ACTGAAGATC TTGCAACGTT GCAGGAAAAA 
AATCAAAACC CAAGAGTTGG TGTGTCTGAT CATTCTCAGC AAGAAGATGG GAGGGCCCAG 
AGACCTGAAG AACATACCAG GCAGCTGGTC AAGAAGCAAG ACACCATTTT AAAACTGCAA 
AAGGATTCAG TTGTTTCTCC CAACTCTGAT ACCCAGGAAG CTGACAAACT CAAACAAACC 
TCAAACATGA AAACAAAGAA TGAAGGCGAC TCTGTTAGTC CAACACAAGA GCAGGTAGCA 
GAACAGGACT CCTCTGTACT TTCTACAAAG AGTCTCAGGG AAGAGCGTTT TCCCAAACCT 
GAGCTAGAAA TCAGCTTCAC GCCACTAAAA CCTCACCGAA TGAATGTCAG GAAACCGGGC 
GAAGAAGAGT CTGTCACGAT CAAACTCCGA CGTACAGCCA GAAAGAGAAA GAGTACTGAG 
ATAGAGAACT CAGTGGAGTC AGAAAACTGT AAAAACCGGA GACTGAAGGT GAACAACAGG 
GCTAACATGC AATCTGTTGA GACTCCCACA GTGCAAGGAA AAAAAATGAG TCAATTAAAG 
CAAAAAGACT CTCCCGCTAG CCTGAAAGGT AAAAAAGACG GACCCCTGCA GAAGATTGGC 
GACTTTATTC AGAGCTCTCC AACCCTCTTT GGGAGCAAAG CCAAGAAGAT CATGAGCATG 
GTGAATGTTA AATCCCCCGA ACCACTAAAT GCATCTGAAA ACAACAAGCC CAAGAGATCC 
AAACGCAAGC TCTTCAAGAC TCAAGTTTCA TCTCCGATGG ACATACCATC CTATCCTATC 
ATTGGAAGCA GTGATGATGA TAAAGAGAGC GATCATCTCA TCATCAAGAG AAAACTCAGA 
ACAAGAACTG CCAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20bb ( XM_003199582.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003199630.3 Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X2, mRNA.

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CloneID	ODa48284
Clone ID Related Accession (Same CDS sequence)	XM_003199582.4 , XM_003199582.5
Accession Version	XM_003199582.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF20B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003199582.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAT CGTGTTTAAA TCACAAGCCT GACCGTGTCG ACTCTATAAC GGTTGAAGAC 
CTCCGAAAAG ATCTTTTCTC CGAGTTCTCC AAATTATCCA GGGACTCGGT GTCGCTTGAA 
AAAGAGCATT TGCAGGTCTA TCTCCGCGTA CGTCCGTTCA CCTCAACAGA GAGAGCTGAA 
GGAGAATCAC AGGATTGTAT CTCCATTGAG CCTCCAGATA CTGTGATTCT GAAGGCACCA 
AAAGCATCTC TGACTGCCAG ACACAGTGAG AGATTTGGAC CACAACTGGC ACAACGATTT 
CAGTTCTCAC AAGTGTACGG TCCCGAAACA ACTCAGAAAG AGATTTTTGA TGGAACAACC 
AAAAGTCTTG TTAAAGAAGT TTTGGACGGA GGGAATTCCT TGATTTTTAC ATATGGAGTT 
ACCAATGCTG GGAAGACATT TACTTTTCTT GGCCCTGAAT CAGATGGTGG AATTTTGCCC 
CGGTCATTAA ACGTGATTTT TAACAGCATA GAAGGCAGAA TTTATTTGCA AAATAACATC 
AAACCTCACC GATGTGTTGA CTTCACAAGA CTCACCAAAG AGCAGCAAGA TGAGGAAGCT 
ACAAATAAAA GAAACCTTCT GCGGCGATTT AAAGATAATG ACTCCCAGAA AACTTTGTCC 
AGCATGTCCA GCTCAAGTTG TTCATCGTTT GACAGCTCCA CATCTAGTGA CATGAATGGA 
GATAGTGTCT ATTTGGATGA GGACTCTCAT GTTAAATTCT CAGTGTGGGT CTCATTTTGT 
GAAATCTACA ATGAAAGCAT CCATGATCTG CTTGATTTTG TTCCAAATGG CTCTCACAGA 
AGGAATGCAT TGCGGTTAGC CCAAGATGTT AAAAGCAATT CTTTTGTTAA AGATCTAAAA 
TGGGTTCAGG TTAACAGTGC AGATGAAGCA TTAAAAGTTG TGAAAATTGG TAGGAAAAAT 
CAAAGTTTCT CATCCACCAA ACTCAACAAC ATCTCAAGCA GAAGCCACAG TATATTTTCC 
ATTCGGATGC TAAGGATTGA AGATGTGGGT GTTCCTCGAG TCCAGACAAT AAGCGAGTTG 
TCATTATGTG ATTTGGCTGG ATCTGAGAGA TGTGCAAGGA CTCAGAATAG AGGAGAACGC 
TTAAAAGAAG CTGGGAATAT CAACACCTCC CTGTTAACTC TAGGAAAATG CATCAATGCC 
CTGAGGCTCA ACCAAACTCA GTCCAAGTTT CATCAGCACA TCCCCTTCAG AGAGAGCAAG 
CTCACACATT ATTTGCAGGG GTATTTCTGT GGCCGTGGAA AAGCTTGCAT GATCATTAAC 
ATAAACCAGT GTGCTTCAGT GTATGATGAA ACTCTGAACG TTCTCAAGTT CTCAGCAGTG 
GCTCAGAAGG TAGTTGTCCT CAACCCTAAA CCAGCTCCAA GCATTGCACT CAAAAGATCT 
GCCAGAGATG TGTCCATGAT AATCAACAAT GCTGACAAGA AGGATTGGAC CAGGAGAAGC 
TCGATTATGG GCTGGGAGAT GAGCCTAGAG GACGTGCAGG AAGATGAAAA TGATGAAGAC 
ATGGATGAGG AAGAGGTGGA GAGTGACGAT GAGAGCATAG TGGACAGAAC TGTGCTAGAG 
ACTGTGGAAA ATCAAGAGCT GTGTGAGCTT CAGGTAAAAA TTGAAGAACT CCAGGAGAAG 
CTGATCAAGG AAGAGTCTGA AAAACTAACT TTGGAAGCCC GTATTCGTGA AGAAGTCACC 
ACAGAGTTTA TGGAGCTGTT TTCTAACATG GAAAAAGACT ACAGTGAACG TCTCCAAAGA 
GAGAAAGAAA TTGTTGAAGA AAGAGCAGAA AGGAGGCTGG AAATCCTGAA GAATCTGGTC 
AACAGGACAA TTGAGGAAAT GACTACAGAT GAAAATGCAG CAAAGGAAGC CAAGATGGAG 
CTTTTGGATA CCATGATCGA GGCAATGCGA AGCGATCTAA CTAAGATAAA GGGAGATGCA 
GAGTCTGTGC AGCTGTGTCT GGCCAATGTC CCTGAATCAC CTGGAAAACT CAGTCATCTG 
AGGACAGAAT TGGAGGAGAT AAAGGCGGAA CTTCTGAAAA GTCAACAACA ACTCAGTCTC 
AAATCTAAAG AGTTTGAAGA AAGCTGTATT CAGATGCAGG ATTTAAATGA CCAACTTCTC 
CAGGCAACCA GGAATTATGA AAACCAGAAA GTGAGATGTC AGGAACTCAT GTCTATCTGC 
CAGGAGAAAG ACGACATGGT ATCTAAATTA CAAACCGCTT TGGAACAAAA TGATGAGGCA 
GCTAGTAAGG ATAGGGCCCT AATTAACAAC ATCAGAGAAG AAATCCTACA TTTCAAAAAC 
AGCTGTAAGT GTAAGCTGAA TGAAGAGCAT GATGAAATGA CAAGTCAGAA AAATCAACAG 
CTTCAGCAAG AACTGAAAAT GAAAGATGAC CAGCTAAATC AACTTAAACT TGAGCATGCA 
TCACTTGAGA AAGCGATGCG AGATATCTCT GATAGATTGT CAGAGCTAAC TCAAACACAT 
GAAGCTACTG TAGCAATGGA GAGAGCAGAG GTCAACAAAG TCACAAATGA GAACAAGGCT 
CTTGCCAACG AGTTGGATGA ACTTCAACAG AGAGCCAATG AGATGAGCTC AAAGGTCGAA 
ACGATCCAAA CTGAATTGGG CAATCAGTCA AAGAAGGCCA ATGAGCTTTC AGAAGAACTC 
CATACTGCCA GGGCTCTGAT CAGGGAACAT CAAGAAGAGT CTTGCAGTCA CTCCCAAACT 
ATTGAATCAT TAATGATAGA GGCTGCTCAT TTGCGTCTAG AACTCAGTAC ATGCCAGCTG 
TCACGTGATA GAAAGGAAGC TGAATGTGAA AGAATGGTCG CGTTATCTCA GGAGAAAAAC 
AGACAGATCA GTGACCTGGA GCAGGAGCTC AAGCAAACCA GAGAAAGTGT GTGTCAACTA 
GAGGAACTCT GTAGTCGGCT TAAAAGTGAG CGTGATGCCC AGGCTCAAGA GTACCAGAGC 
CTTCTAAACC GTTACGAAGC ACAAAATTTA GTTGTGACTC AAATAAGAAG CAACTCTGAT 
CTTTTGGAGG AGCTGACTGC TCTAAAACAT CAGCACAAGA AAATTGAAAA ACAAGATGAT 
TGCTGGAAAA CCGTGCAGGA CAACCTCATA CAAACAGTGA GCAAACTAAA CCAGCAAGAG 
GAGATTCTGA GCATAGTGCA AACAATTGTA ACAAATGAGA TTTCTAAGGC ACGAGATGAG 
GCAAATATGA AGATAACTGA AATGGAGAAG GATCTGTCTA ACAAAAACAC AGAGCTAGAG 
ACAAAAGCCC AAGAGCTTAT GAAAGTGAGA GAACATGTGA ACGATGGCCA CAATCAAATT 
GAGCGCATGC GCTGTGATTT GCAGCAGGTG GAGCAGGAGA GATCTGATGT CAGAGATCAG 
TTATGTGAGG CTAACACTCA GAAAGAACAT TTAGAGAAAC AGATTTTAAT CTTGAGTGAA 
GAAAGCAAAA TGTTTCAGCA GAGGCTGTGT GAGGCAGAGA AGATGAAGGA TCAGCCAAGA 
CCCTGTTTGA GCTGTATAGA GCAAACTATT GATCCACTGC AGATTGAGCA GTCTAACCAT 
GTATTAGAAA AGGAAGATAT ACAGCTTTTC CAAACACCCT GTCAAGAGAG TAAGTTGATT 
GAAGAGACTC CTGTAACCTT AAACACAGAG TTTAAACGGA CAGATGAGGA GGAATTGTTG 
AAGCCCAGAC TGACTGAGAT TGAATTCATA ACTGAAGATC TTGCAACGTT GCAGGAAAAA 
AATCAAAACC CAAGAGTTGG TGTGTCTGAT CATTCTCAGC AAGAAGATGG GAGGGCCCAG 
AGACCTGAAG AACATACCAG GCAGCTGGTC AAGAAGCAAG ACACCATTTT AAAACTGCAA 
AAGGATTCAG TTGTTTCTCC CAACTCTGAT ACCCAGGAAG CTGACAAACT CAAACAAACC 
TCAAACATGA AAACAAAGAA TGAAGGCGAC TCTGTTAGTC CAACACAAGA GCAGGTAGCA 
GAACAGGACT CCTCTGTACT TTCTACAAAG AGTCTCAGGG AAGAGCGTTT TCCCAAACCT 
GAGCTAGAAA TCAGCTTCAC GCCACTAAAA CCTCACCGAA TGAATGTCAG GAAACCGGGC 
GAAGAAGAGT CTGTCACGAT CAAACTCCGA CGTACAGCCA GAAAGAGAAA GAGTACTGAG 
ATAGAGAACT CAGTGGAGTC AGAAAACTGT AAAAACCGGA GACTGAAGGT GAACAACAGG 
GCTAACATGC AATCTGTTGA GACTCCCACA GTGCAAGGAA AAAAAATGAG TCAATTAAAG 
CAAAAAGACT CTCCCGCTAG CCTGAAAGGT AAAAAAGACG GACCCCTGCA GAAGATTGGC 
GACTTTATTC AGAGCTCTCC AACCCTCTTT GGGAGCAAAG CCAAGAAGAT CATGAGCATG 
GTGAATGTTA AATCCCCCGA ACCACTAAAT GCATCTGAAA ACAACAAGCC CAAGAGATCC 
AAACGCAAGC TCTTCAAGAC TCAAGTTTCA TCTCCGATGG ACATACCATC CTATCCTATC 
ATTGGAAGCA GTGATGATGA TAAAGAGAGC GATCATCTCA TCATCAAGAG AAAACTCAGA 
ACAAGAACTG CCAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003199630.3
CDS	106..4746
Translation	MMESCLNHKPDRVDSITVEDLRKDLFSEFSKLSRDSVSLEKEHLQVYLRVRPFTSTERAEGESQDCISIEPPDTVILKAPKASLTARHSERFGPQLAQRFQFSQVYGPETTQKEIFDGTTKSLVKEVLDGGNSLIFTYGVTNAGKTFTFLGPESDGGILPRSLNVIFNSIEGRIYLQNNIKPHRCVDFTRLTKEQQDEEATNKRNLLRRFKDNDSQKTLSSMSSSSCSSFDSSTSSDMNGDSVYLDEDSHVKFSVWVSFCEIYNESIHDLLDFVPNGSHRRNALRLAQDVKSNSFVKDLKWVQVNSADEALKVVKIGRKNQSFSSTKLNNISSRSHSIFSIRMLRIEDVGVPRVQTISELSLCDLAGSERCARTQNRGERLKEAGNINTSLLTLGKCINALRLNQTQSKFHQHIPFRESKLTHYLQGYFCGRGKACMIININQCASVYDETLNVLKFSAVAQKVVVLNPKPAPSIALKRSARDVSMIINNADKKDWTRRSSIMGWEMSLEDVQEDENDEDMDEEEVESDDESIVDRTVLETVENQELCELQVKIEELQEKLIKEESEKLTLEARIREEVTTEFMELFSNMEKDYSERLQREKEIVEERAERRLEILKNLVNRTIEEMTTDENAAKEAKMELLDTMIEAMRSDLTKIKGDAESVQLCLANVPESPGKLSHLRTELEEIKAELLKSQQQLSLKSKEFEESCIQMQDLNDQLLQATRNYENQKVRCQELMSICQEKDDMVSKLQTALEQNDEAASKDRALINNIREEILHFKNSCKCKLNEEHDEMTSQKNQQLQQELKMKDDQLNQLKLEHASLEKAMRDISDRLSELTQTHEATVAMERAEVNKVTNENKALANELDELQQRANEMSSKVETIQTELGNQSKKANELSEELHTARALIREHQEESCSHSQTIESLMIEAAHLRLELSTCQLSRDRKEAECERMVALSQEKNRQISDLEQELKQTRESVCQLEELCSRLKSERDAQAQEYQSLLNRYEAQNLVVTQIRSNSDLLEELTALKHQHKKIEKQDDCWKTVQDNLIQTVSKLNQQEEILSIVQTIVTNEISKARDEANMKITEMEKDLSNKNTELETKAQELMKVREHVNDGHNQIERMRCDLQQVEQERSDVRDQLCEANTQKEHLEKQILILSEESKMFQQRLCEAEKMKDQPRPCLSCIEQTIDPLQIEQSNHVLEKEDIQLFQTPCQESKLIEETPVTLNTEFKRTDEEELLKPRLTEIEFITEDLATLQEKNQNPRVGVSDHSQQEDGRAQRPEEHTRQLVKKQDTILKLQKDSVVSPNSDTQEADKLKQTSNMKTKNEGDSVSPTQEQVAEQDSSVLSTKSLREERFPKPELEISFTPLKPHRMNVRKPGEEESVTIKLRRTARKRKSTEIENSVESENCKNRRLKVNNRANMQSVETPTVQGKKMSQLKQKDSPASLKGKKDGPLQKIGDFIQSSPTLFGSKAKKIMSMVNVKSPEPLNASENNKPKRSKRKLFKTQVSSPMDIPSYPIIGSSDDDKESDHLIIKRKLRTRTAKR

Target ORF information:

RefSeq Version	XM_003199582.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003199582.5

ORF Insert Sequence:

ATGATGGAAT CGTGTTTAAA TCACAAGCCT GACCGTGTCG ACTCTATAAC GGTTGAAGAC 
CTCCGAAAAG ATCTTTTCTC CGAGTTCTCC AAATTATCCA GGGACTCGGT GTCGCTTGAA 
AAAGAGCATT TGCAGGTCTA TCTCCGCGTA CGTCCGTTCA CCTCAACAGA GAGAGCTGAA 
GGAGAATCAC AGGATTGTAT CTCCATTGAG CCTCCAGATA CTGTGATTCT GAAGGCACCA 
AAAGCATCTC TGACTGCCAG ACACAGTGAG AGATTTGGAC CACAACTGGC ACAACGATTT 
CAGTTCTCAC AAGTGTACGG TCCCGAAACA ACTCAGAAAG AGATTTTTGA TGGAACAACC 
AAAAGTCTTG TTAAAGAAGT TTTGGACGGA GGGAATTCCT TGATTTTTAC ATATGGAGTT 
ACCAATGCTG GGAAGACATT TACTTTTCTT GGCCCTGAAT CAGATGGTGG AATTTTGCCC 
CGGTCATTAA ACGTGATTTT TAACAGCATA GAAGGCAGAA TTTATTTGCA AAATAACATC 
AAACCTCACC GATGTGTTGA CTTCACAAGA CTCACCAAAG AGCAGCAAGA TGAGGAAGCT 
ACAAATAAAA GAAACCTTCT GCGGCGATTT AAAGATAATG ACTCCCAGAA AACTTTGTCC 
AGCATGTCCA GCTCAAGTTG TTCATCGTTT GACAGCTCCA CATCTAGTGA CATGAATGGA 
GATAGTGTCT ATTTGGATGA GGACTCTCAT GTTAAATTCT CAGTGTGGGT CTCATTTTGT 
GAAATCTACA ATGAAAGCAT CCATGATCTG CTTGATTTTG TTCCAAATGG CTCTCACAGA 
AGGAATGCAT TGCGGTTAGC CCAAGATGTT AAAAGCAATT CTTTTGTTAA AGATCTAAAA 
TGGGTTCAGG TTAACAGTGC AGATGAAGCA TTAAAAGTTG TGAAAATTGG TAGGAAAAAT 
CAAAGTTTCT CATCCACCAA ACTCAACAAC ATCTCAAGCA GAAGCCACAG TATATTTTCC 
ATTCGGATGC TAAGGATTGA AGATGTGGGT GTTCCTCGAG TCCAGACAAT AAGCGAGTTG 
TCATTATGTG ATTTGGCTGG ATCTGAGAGA TGTGCAAGGA CTCAGAATAG AGGAGAACGC 
TTAAAAGAAG CTGGGAATAT CAACACCTCC CTGTTAACTC TAGGAAAATG CATCAATGCC 
CTGAGGCTCA ACCAAACTCA GTCCAAGTTT CATCAGCACA TCCCCTTCAG AGAGAGCAAG 
CTCACACATT ATTTGCAGGG GTATTTCTGT GGCCGTGGAA AAGCTTGCAT GATCATTAAC 
ATAAACCAGT GTGCTTCAGT GTATGATGAA ACTCTGAACG TTCTCAAGTT CTCAGCAGTG 
GCTCAGAAGG TAGTTGTCCT CAACCCTAAA CCAGCTCCAA GCATTGCACT CAAAAGATCT 
GCCAGAGATG TGTCCATGAT AATCAACAAT GCTGACAAGA AGGATTGGAC CAGGAGAAGC 
TCGATTATGG GCTGGGAGAT GAGCCTAGAG GACGTGCAGG AAGATGAAAA TGATGAAGAC 
ATGGATGAGG AAGAGGTGGA GAGTGACGAT GAGAGCATAG TGGACAGAAC TGTGCTAGAG 
ACTGTGGAAA ATCAAGAGCT GTGTGAGCTT CAGGTAAAAA TTGAAGAACT CCAGGAGAAG 
CTGATCAAGG AAGAGTCTGA AAAACTAACT TTGGAAGCCC GTATTCGTGA AGAAGTCACC 
ACAGAGTTTA TGGAGCTGTT TTCTAACATG GAAAAAGACT ACAGTGAACG TCTCCAAAGA 
GAGAAAGAAA TTGTTGAAGA AAGAGCAGAA AGGAGGCTGG AAATCCTGAA GAATCTGGTC 
AACAGGACAA TTGAGGAAAT GACTACAGAT GAAAATGCAG CAAAGGAAGC CAAGATGGAG 
CTTTTGGATA CCATGATCGA GGCAATGCGA AGCGATCTAA CTAAGATAAA GGGAGATGCA 
GAGTCTGTGC AGCTGTGTCT GGCCAATGTC CCTGAATCAC CTGGAAAACT CAGTCATCTG 
AGGACAGAAT TGGAGGAGAT AAAGGCGGAA CTTCTGAAAA GTCAACAACA ACTCAGTCTC 
AAATCTAAAG AGTTTGAAGA AAGCTGTATT CAGATGCAGG ATTTAAATGA CCAACTTCTC 
CAGGCAACCA GGAATTATGA AAACCAGAAA GTGAGATGTC AGGAACTCAT GTCTATCTGC 
CAGGAGAAAG ACGACATGGT ATCTAAATTA CAAACCGCTT TGGAACAAAA TGATGAGGCA 
GCTAGTAAGG ATAGGGCCCT AATTAACAAC ATCAGAGAAG AAATCCTACA TTTCAAAAAC 
AGCTGTAAGT GTAAGCTGAA TGAAGAGCAT GATGAAATGA CAAGTCAGAA AAATCAACAG 
CTTCAGCAAG AACTGAAAAT GAAAGATGAC CAGCTAAATC AACTTAAACT TGAGCATGCA 
TCACTTGAGA AAGCGATGCG AGATATCTCT GATAGATTGT CAGAGCTAAC TCAAACACAT 
GAAGCTACTG TAGCAATGGA GAGAGCAGAG GTCAACAAAG TCACAAATGA GAACAAGGCT 
CTTGCCAACG AGTTGGATGA ACTTCAACAG AGAGCCAATG AGATGAGCTC AAAGGTCGAA 
ACGATCCAAA CTGAATTGGG CAATCAGTCA AAGAAGGCCA ATGAGCTTTC AGAAGAACTC 
CATACTGCCA GGGCTCTGAT CAGGGAACAT CAAGAAGAGT CTTGCAGTCA CTCCCAAACT 
ATTGAATCAT TAATGATAGA GGCTGCTCAT TTGCGTCTAG AACTCAGTAC ATGCCAGCTG 
TCACGTGATA GAAAGGAAGC TGAATGTGAA AGAATGGTCG CGTTATCTCA GGAGAAAAAC 
AGACAGATCA GTGACCTGGA GCAGGAGCTC AAGCAAACCA GAGAAAGTGT GTGTCAACTA 
GAGGAACTCT GTAGTCGGCT TAAAAGTGAG CGTGATGCCC AGGCTCAAGA GTACCAGAGC 
CTTCTAAACC GTTACGAAGC ACAAAATTTA GTTGTGACTC AAATAAGAAG CAACTCTGAT 
CTTTTGGAGG AGCTGACTGC TCTAAAACAT CAGCACAAGA AAATTGAAAA ACAAGATGAT 
TGCTGGAAAA CCGTGCAGGA CAACCTCATA CAAACAGTGA GCAAACTAAA CCAGCAAGAG 
GAGATTCTGA GCATAGTGCA AACAATTGTA ACAAATGAGA TTTCTAAGGC ACGAGATGAG 
GCAAATATGA AGATAACTGA AATGGAGAAG GATCTGTCTA ACAAAAACAC AGAGCTAGAG 
ACAAAAGCCC AAGAGCTTAT GAAAGTGAGA GAACATGTGA ACGATGGCCA CAATCAAATT 
GAGCGCATGC GCTGTGATTT GCAGCAGGTG GAGCAGGAGA GATCTGATGT CAGAGATCAG 
TTATGTGAGG CTAACACTCA GAAAGAACAT TTAGAGAAAC AGATTTTAAT CTTGAGTGAA 
GAAAGCAAAA TGTTTCAGCA GAGGCTGTGT GAGGCAGAGA AGATGAAGGA TCAGCCAAGA 
CCCTGTTTGA GCTGTATAGA GCAAACTATT GATCCACTGC AGATTGAGCA GTCTAACCAT 
GTATTAGAAA AGGAAGATAT ACAGCTTTTC CAAACACCCT GTCAAGAGAG TAAGTTGATT 
GAAGAGACTC CTGTAACCTT AAACACAGAG TTTAAACGGA CAGATGAGGA GGAATTGTTG 
AAGCCCAGAC TGACTGAGAT TGAATTCATA ACTGAAGATC TTGCAACGTT GCAGGAAAAA 
AATCAAAACC CAAGAGTTGG TGTGTCTGAT CATTCTCAGC AAGAAGATGG GAGGGCCCAG 
AGACCTGAAG AACATACCAG GCAGCTGGTC AAGAAGCAAG ACACCATTTT AAAACTGCAA 
AAGGATTCAG TTGTTTCTCC CAACTCTGAT ACCCAGGAAG CTGACAAACT CAAACAAACC 
TCAAACATGA AAACAAAGAA TGAAGGCGAC TCTGTTAGTC CAACACAAGA GCAGGTAGCA 
GAACAGGACT CCTCTGTACT TTCTACAAAG AGTCTCAGGG AAGAGCGTTT TCCCAAACCT 
GAGCTAGAAA TCAGCTTCAC GCCACTAAAA CCTCACCGAA TGAATGTCAG GAAACCGGGC 
GAAGAAGAGT CTGTCACGAT CAAACTCCGA CGTACAGCCA GAAAGAGAAA GAGTACTGAG 
ATAGAGAACT CAGTGGAGTC AGAAAACTGT AAAAACCGGA GACTGAAGGT GAACAACAGG 
GCTAACATGC AATCTGTTGA GACTCCCACA GTGCAAGGAA AAAAAATGAG TCAATTAAAG 
CAAAAAGACT CTCCCGCTAG CCTGAAAGGT AAAAAAGACG GACCCCTGCA GAAGATTGGC 
GACTTTATTC AGAGCTCTCC AACCCTCTTT GGGAGCAAAG CCAAGAAGAT CATGAGCATG 
GTGAATGTTA AATCCCCCGA ACCACTAAAT GCATCTGAAA ACAACAAGCC CAAGAGATCC 
AAACGCAAGC TCTTCAAGAC TCAAGTTTCA TCTCCGATGG ACATACCATC CTATCCTATC 
ATTGGAAGCA GTGATGATGA TAAAGAGAGC GATCATCTCA TCATCAAGAG AAAACTCAGA 
ACAAGAACTG CCAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20bb ( XM_009306554.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304829.1 Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X2, mRNA.

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CloneID	ODa48286
Clone ID Related Accession (Same CDS sequence)	XM_009306554.2 , XM_009306554.3
Accession Version	XM_009306554.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF20B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306554.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAT CGTGTTTAAA TCACAAGCCT GACCGTGTCG ACTCTATAAC GGTTGAAGAC 
CTCCGAAAAG ATCTTTTCTC CGAGTTCTCC AAATTATCCA GGGACTCGGT GTCGCTTGAA 
AAAGAGCATT TGCAGGTCTA TCTCCGCGTA CGTCCGTTCA CCTCAACAGA GAGAGCTGAA 
GGAGAATCAC AGGATTGTAT CTCCATTGAG CCTCCAGATA CTGTGATTCT GAAGGCACCA 
AAAGCATCTC TGACTGCCAG ACACAGTGAG AGATTTGGAC CACAACTGGC ACAACGATTT 
CAGTTCTCAC AAGTGTACGG TCCCGAAACA ACTCAGAAAG AGATTTTTGA TGGAACAACC 
AAAAGTCTTG TTAAAGAAGT TTTGGACGGA GGGAATTCCT TGATTTTTAC ATATGGAGTT 
ACCAATGCTG GGAAGACATT TACTTTTCTT GGCCCTGAAT CAGATGGTGG AATTTTGCCC 
CGGTCATTAA ACGTGATTTT TAACAGCATA GAAGGCAGAA TTTATTTGCA AAATAACATC 
AAACCTCACC GATGTGTTGA CTTCACAAGA CTCACCAAAG AGCAGCAAGA TGAGGAAGCT 
ACAAATAAAA GAAACCTTCT GCGGCGATTT AAAGATAATG ACTCCCAGAA AACTTTGTCC 
AGCATGTCCA GCTCAAGTTG TTCATCGTTT GACAGCTCCA CATCTAGTGA CATGAATGGA 
GATAGTGTCT ATTTGGATGA GGACTCTCAT GTTAAATTCT CAGTGTGGGT CTCATTTTGT 
GAAATCTACA ATGAAAGCAT CCATGATCTG CTTGATTTTG TTCCAAATGG CTCTCACAGA 
AGGAATGCAT TGCGGTTAGC CCAAGATGTT AAAAGCAATT CTTTTGTTAA AGATCTAAAA 
TGGGTTCAGG TTAACAGTGC AGATGAAGCA TTAAAAGTTG TGAAAATTGG TAGGAAAAAT 
CAAAGTTTCT CATCCACCAA ACTCAACAAC ATCTCAAGCA GAAGCCACAG TATATTTTCC 
ATTCGGATGC TAAGGATTGA AGATGTGGGT GTTCCTCGAG TCCAGACAAT AAGCGAGTTG 
TCATTATGTG ATTTGGCTGG ATCTGAGAGA TGTGCAAGGA CTCAGAATAG AGGAGAACGC 
TTAAAAGAAG CTGGGAATAT CAACACCTCC CTGTTAACTC TAGGAAAATG CATCAATGCC 
CTGAGGCTCA ACCAAACTCA GTCCAAGTTT CATCAGCACA TCCCCTTCAG AGAGAGCAAG 
CTCACACATT ATTTGCAGGG GTATTTCTGT GGCCGTGGAA AAGCTTGCAT GATCATTAAC 
ATAAACCAGT GTGCTTCAGT GTATGATGAA ACTCTGAACG TTCTCAAGTT CTCAGCAGTG 
GCTCAGAAGG TAGTTGTCCT CAACCCTAAA CCAGCTCCAA GCATTGCACT CAAAAGATCT 
GCCAGAGATG TGTCCATGAT AATCAACAAT GCTGACAAGA AGGATTGGAC CAGGAGAAGC 
TCGATTATGG GCTGGGAGAT GAGCCTAGAG GACGTGCAGG AAGATGAAAA TGATGAAGAC 
ATGGATGAGG AAGAGGTGGA GAGTGACGAT GAGAGCATAG TGGACAGAAC TGTGCTAGAG 
ACTGTGGAAA ATCAAGAGCT GTGTGAGCTT CAGGTAAAAA TTGAAGAACT CCAGGAGAAG 
CTGATCAAGG AAGAGTCTGA AAAACTAACT TTGGAAGCCC GTATTCGTGA AGAAGTCACC 
ACAGAGTTTA TGGAGCTGTT TTCTAACATG GAAAAAGACT ACAGTGAACG TCTCCAAAGA 
GAGAAAGAAA TTGTTGAAGA AAGAGCAGAA AGGAGGCTGG AAATCCTGAA GAATCTGGTC 
AACAGGACAA TTGAGGAAAT GACTACAGAT GAAAATGCAG CAAAGGAAGC CAAGATGGAG 
CTTTTGGATA CCATGATCGA GGCAATGCGA AGCGATCTAA CTAAGATAAA GGGAGATGCA 
GAGTCTGTGC AGCTGTGTCT GGCCAATGTC CCTGAATCAC CTGGAAAACT CAGTCATCTG 
AGGACAGAAT TGGAGGAGAT AAAGGCGGAA CTTCTGAAAA GTCAACAACA ACTCAGTCTC 
AAATCTAAAG AGTTTGAAGA AAGCTGTATT CAGATGCAGG ATTTAAATGA CCAACTTCTC 
CAGGCAACCA GGAATTATGA AAACCAGAAA GTGAGATGTC AGGAACTCAT GTCTATCTGC 
CAGGAGAAAG ACGACATGGT ATCTAAATTA CAAACCGCTT TGGAACAAAA TGATGAGGCA 
GCTAGTAAGG ATAGGGCCCT AATTAACAAC ATCAGAGAAG AAATCCTACA TTTCAAAAAC 
AGCTGTAAGT GTAAGCTGAA TGAAGAGCAT GATGAAATGA CAAGTCAGAA AAATCAACAG 
CTTCAGCAAG AACTGAAAAT GAAAGATGAC CAGCTAAATC AACTTAAACT TGAGCATGCA 
TCACTTGAGA AAGCGATGCG AGATATCTCT GATAGATTGT CAGAGCTAAC TCAAACACAT 
GAAGCTACTG TAGCAATGGA GAGAGCAGAG GTCAACAAAG TCACAAATGA GAACAAGGCT 
CTTGCCAACG AGTTGGATGA ACTTCAACAG AGAGCCAATG AGATGAGCTC AAAGGTCGAA 
ACGATCCAAA CTGAATTGGG CAATCAGTCA AAGAAGGCCA ATGAGCTTTC AGAAGAACTC 
CATACTGCCA GGGCTCTGAT CAGGGAACAT CAAGAAGAGT CTTGCAGTCA CTCCCAAACT 
ATTGAATCAT TAATGATAGA GGCTGCTCAT TTGCGTCTAG AACTCAGTAC ATGCCAGCTG 
TCACGTGATA GAAAGGAAGC TGAATGTGAA AGAATGGTCG CGTTATCTCA GGAGAAAAAC 
AGACAGATCA GTGACCTGGA GCAGGAGCTC AAGCAAACCA GAGAAAGTGT GTGTCAACTA 
GAGGAACTCT GTAGTCGGCT TAAAAGTGAG CGTGATGCCC AGGCTCAAGA GTACCAGAGC 
CTTCTAAACC GTTACGAAGC ACAAAATTTA GTTGTGACTC AAATAAGAAG CAACTCTGAT 
CTTTTGGAGG AGCTGACTGC TCTAAAACAT CAGCACAAGA AAATTGAAAA ACAAGATGAT 
TGCTGGAAAA CCGTGCAGGA CAACCTCATA CAAACAGTGA GCAAACTAAA CCAGCAAGAG 
GAGATTCTGA GCATAGTGCA AACAATTGTA ACAAATGAGA TTTCTAAGGC ACGAGATGAG 
GCAAATATGA AGATAACTGA AATGGAGAAG GATCTGTCTA ACAAAAACAC AGAGCTAGAG 
ACAAAAGCCC AAGAGCTTAT GAAAGTGAGA GAACATGTGA ACGATGGCCA CAATCAAATT 
GAGCGCATGC GCTGTGATTT GCAGCAGGTG GAGCAGGAGA GATCTGATGT CAGAGATCAG 
TTATGTGAGG CTAACACTCA GAAAGAACAT TTAGAGAAAC AGATTTTAAT CTTGAGTGAA 
GAAAGCAAAA TGTTTCAGCA GAGGCTGTGT GAGGCAGAGA AGATGAAGGA TCAGCCAAGA 
CCCTGTTTGA GCTGTATAGA GCAAACTATT GATCCACTGC AGATTGAGCA GTCTAACCAT 
GTATTAGAAA AGGAAGATAT ACAGCTTTTC CAAACACCCT GTCAAGAGAG TAAGTTGATT 
GAAGAGACTC CTGTAACCTT AAACACAGAG TTTAAACGGA CAGATGAGGA GGAATTGTTG 
AAGCCCAGAC TGACTGAGAT TGAATTCATA ACTGAAGATC TTGCAACGTT GCAGGAAAAA 
AATCAAAACC CAAGAGTTGG TGTGTCTGAT CATTCTCAGC AAGAAGATGG GAGGGCCCAG 
AGACCTGAAG AACATACCAG GCAGCTGGTC AAGAAGCAAG ACACCATTTT AAAACTGCAA 
AAGGATTCAG TTGTTTCTCC CAACTCTGAT ACCCAGGAAG CTGACAAACT CAAACAAACC 
TCAAACATGA AAACAAAGAA TGAAGGCGAC TCTGTTAGTC CAACACAAGA GGTAGCAGAA 
CAGGACTCCT CTGTACTTTC TACAAAGAGT CTCAGGGAAG AGCGTTTTCC CAAACCTGAG 
CTAGAAATCA GCTTCACGCC ACTAAAACCT CACCGAATGA ATGTCAGGAA ACCGGGCGAA 
GAAGAGTCTG TCACGATCAA ACTCCGACGT ACAGCCAGAA AGAGAAAGAG TACTGAGATA 
GAGAACTCAG TGGAGTCAGA AAACTGTAAA AACCGGAGAC TGAAGGTGAA CAACAGGGCT 
AACATGCAAT CTGTTGAGAC TCCCACAGTG CAAGGAAAAA AAATGAGTCA ATTAAAGCAA 
AAAGACTCTC CCGCTAGCCT GAAAGGTAAA AAAGACGGAC CCCTGCAGAA GATTGGCGAC 
TTTATTCAGA GCTCTCCAAC CCTCTTTGGG AGCAAAGCCA AGAAGATCAT GAGCATGGTG 
AATGTTAAAT CCCCCGAACC ACTAAATGCA TCTGAAAACA ACAAGCCCAA GAGATCCAAA 
CGCAAGCTCT TCAAGACTCA AGTTTCATCT CCGATGGACA TACCATCCTA TCCTATCATT 
GGAAGCAGTG ATGATGATAA AGAGAGCGAT CATCTCATCA TCAAGAGAAA ACTCAGAACA 
AGAACTGCCA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304829.1
CDS	109..4746
Translation	MMESCLNHKPDRVDSITVEDLRKDLFSEFSKLSRDSVSLEKEHLQVYLRVRPFTSTERAEGESQDCISIEPPDTVILKAPKASLTARHSERFGPQLAQRFQFSQVYGPETTQKEIFDGTTKSLVKEVLDGGNSLIFTYGVTNAGKTFTFLGPESDGGILPRSLNVIFNSIEGRIYLQNNIKPHRCVDFTRLTKEQQDEEATNKRNLLRRFKDNDSQKTLSSMSSSSCSSFDSSTSSDMNGDSVYLDEDSHVKFSVWVSFCEIYNESIHDLLDFVPNGSHRRNALRLAQDVKSNSFVKDLKWVQVNSADEALKVVKIGRKNQSFSSTKLNNISSRSHSIFSIRMLRIEDVGVPRVQTISELSLCDLAGSERCARTQNRGERLKEAGNINTSLLTLGKCINALRLNQTQSKFHQHIPFRESKLTHYLQGYFCGRGKACMIININQCASVYDETLNVLKFSAVAQKVVVLNPKPAPSIALKRSARDVSMIINNADKKDWTRRSSIMGWEMSLEDVQEDENDEDMDEEEVESDDESIVDRTVLETVENQELCELQVKIEELQEKLIKEESEKLTLEARIREEVTTEFMELFSNMEKDYSERLQREKEIVEERAERRLEILKNLVNRTIEEMTTDENAAKEAKMELLDTMIEAMRSDLTKIKGDAESVQLCLANVPESPGKLSHLRTELEEIKAELLKSQQQLSLKSKEFEESCIQMQDLNDQLLQATRNYENQKVRCQELMSICQEKDDMVSKLQTALEQNDEAASKDRALINNIREEILHFKNSCKCKLNEEHDEMTSQKNQQLQQELKMKDDQLNQLKLEHASLEKAMRDISDRLSELTQTHEATVAMERAEVNKVTNENKALANELDELQQRANEMSSKVETIQTELGNQSKKANELSEELHTARALIREHQEESCSHSQTIESLMIEAAHLRLELSTCQLSRDRKEAECERMVALSQEKNRQISDLEQELKQTRESVCQLEELCSRLKSERDAQAQEYQSLLNRYEAQNLVVTQIRSNSDLLEELTALKHQHKKIEKQDDCWKTVQDNLIQTVSKLNQQEEILSIVQTIVTNEISKARDEANMKITEMEKDLSNKNTELETKAQELMKVREHVNDGHNQIERMRCDLQQVEQERSDVRDQLCEANTQKEHLEKQILILSEESKMFQQRLCEAEKMKDQPRPCLSCIEQTIDPLQIEQSNHVLEKEDIQLFQTPCQESKLIEETPVTLNTEFKRTDEEELLKPRLTEIEFITEDLATLQEKNQNPRVGVSDHSQQEDGRAQRPEEHTRQLVKKQDTILKLQKDSVVSPNSDTQEADKLKQTSNMKTKNEGDSVSPTQEVAEQDSSVLSTKSLREERFPKPELEISFTPLKPHRMNVRKPGEEESVTIKLRRTARKRKSTEIENSVESENCKNRRLKVNNRANMQSVETPTVQGKKMSQLKQKDSPASLKGKKDGPLQKIGDFIQSSPTLFGSKAKKIMSMVNVKSPEPLNASENNKPKRSKRKLFKTQVSSPMDIPSYPIIGSSDDDKESDHLIIKRKLRTRTAKR

Target ORF information:

RefSeq Version	XM_009306554.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306554.2

ORF Insert Sequence:

ATGATGGAAT CGTGTTTAAA TCACAAGCCT GACCGTGTCG ACTCTATAAC GGTTGAAGAC 
CTCCGAAAAG ATCTTTTCTC CGAGTTCTCC AAATTATCCA GGGACTCGGT GTCGCTTGAA 
AAAGAGCATT TGCAGGTCTA TCTCCGCGTA CGTCCGTTCA CCTCAACAGA GAGAGCTGAA 
GGAGAATCAC AGGATTGTAT CTCCATTGAG CCTCCAGATA CTGTGATTCT GAAGGCACCA 
AAAGCATCTC TGACTGCCAG ACACAGTGAG AGATTTGGAC CACAACTGGC ACAACGATTT 
CAGTTCTCAC AAGTGTACGG TCCCGAAACA ACTCAGAAAG AGATTTTTGA TGGAACAACC 
AAAAGTCTTG TTAAAGAAGT TTTGGACGGA GGGAATTCCT TGATTTTTAC ATATGGAGTT 
ACCAATGCTG GGAAGACATT TACTTTTCTT GGCCCTGAAT CAGATGGTGG AATTTTGCCC 
CGGTCATTAA ACGTGATTTT TAACAGCATA GAAGGCAGAA TTTATTTGCA AAATAACATC 
AAACCTCACC GATGTGTTGA CTTCACAAGA CTCACCAAAG AGCAGCAAGA TGAGGAAGCT 
ACAAATAAAA GAAACCTTCT GCGGCGATTT AAAGATAATG ACTCCCAGAA AACTTTGTCC 
AGCATGTCCA GCTCAAGTTG TTCATCGTTT GACAGCTCCA CATCTAGTGA CATGAATGGA 
GATAGTGTCT ATTTGGATGA GGACTCTCAT GTTAAATTCT CAGTGTGGGT CTCATTTTGT 
GAAATCTACA ATGAAAGCAT CCATGATCTG CTTGATTTTG TTCCAAATGG CTCTCACAGA 
AGGAATGCAT TGCGGTTAGC CCAAGATGTT AAAAGCAATT CTTTTGTTAA AGATCTAAAA 
TGGGTTCAGG TTAACAGTGC AGATGAAGCA TTAAAAGTTG TGAAAATTGG TAGGAAAAAT 
CAAAGTTTCT CATCCACCAA ACTCAACAAC ATCTCAAGCA GAAGCCACAG TATATTTTCC 
ATTCGGATGC TAAGGATTGA AGATGTGGGT GTTCCTCGAG TCCAGACAAT AAGCGAGTTG 
TCATTATGTG ATTTGGCTGG ATCTGAGAGA TGTGCAAGGA CTCAGAATAG AGGAGAACGC 
TTAAAAGAAG CTGGGAATAT CAACACCTCC CTGTTAACTC TAGGAAAATG CATCAATGCC 
CTGAGGCTCA ACCAAACTCA GTCCAAGTTT CATCAGCACA TCCCCTTCAG AGAGAGCAAG 
CTCACACATT ATTTGCAGGG GTATTTCTGT GGCCGTGGAA AAGCTTGCAT GATCATTAAC 
ATAAACCAGT GTGCTTCAGT GTATGATGAA ACTCTGAACG TTCTCAAGTT CTCAGCAGTG 
GCTCAGAAGG TAGTTGTCCT CAACCCTAAA CCAGCTCCAA GCATTGCACT CAAAAGATCT 
GCCAGAGATG TGTCCATGAT AATCAACAAT GCTGACAAGA AGGATTGGAC CAGGAGAAGC 
TCGATTATGG GCTGGGAGAT GAGCCTAGAG GACGTGCAGG AAGATGAAAA TGATGAAGAC 
ATGGATGAGG AAGAGGTGGA GAGTGACGAT GAGAGCATAG TGGACAGAAC TGTGCTAGAG 
ACTGTGGAAA ATCAAGAGCT GTGTGAGCTT CAGGTAAAAA TTGAAGAACT CCAGGAGAAG 
CTGATCAAGG AAGAGTCTGA AAAACTAACT TTGGAAGCCC GTATTCGTGA AGAAGTCACC 
ACAGAGTTTA TGGAGCTGTT TTCTAACATG GAAAAAGACT ACAGTGAACG TCTCCAAAGA 
GAGAAAGAAA TTGTTGAAGA AAGAGCAGAA AGGAGGCTGG AAATCCTGAA GAATCTGGTC 
AACAGGACAA TTGAGGAAAT GACTACAGAT GAAAATGCAG CAAAGGAAGC CAAGATGGAG 
CTTTTGGATA CCATGATCGA GGCAATGCGA AGCGATCTAA CTAAGATAAA GGGAGATGCA 
GAGTCTGTGC AGCTGTGTCT GGCCAATGTC CCTGAATCAC CTGGAAAACT CAGTCATCTG 
AGGACAGAAT TGGAGGAGAT AAAGGCGGAA CTTCTGAAAA GTCAACAACA ACTCAGTCTC 
AAATCTAAAG AGTTTGAAGA AAGCTGTATT CAGATGCAGG ATTTAAATGA CCAACTTCTC 
CAGGCAACCA GGAATTATGA AAACCAGAAA GTGAGATGTC AGGAACTCAT GTCTATCTGC 
CAGGAGAAAG ACGACATGGT ATCTAAATTA CAAACCGCTT TGGAACAAAA TGATGAGGCA 
GCTAGTAAGG ATAGGGCCCT AATTAACAAC ATCAGAGAAG AAATCCTACA TTTCAAAAAC 
AGCTGTAAGT GTAAGCTGAA TGAAGAGCAT GATGAAATGA CAAGTCAGAA AAATCAACAG 
CTTCAGCAAG AACTGAAAAT GAAAGATGAC CAGCTAAATC AACTTAAACT TGAGCATGCA 
TCACTTGAGA AAGCGATGCG AGATATCTCT GATAGATTGT CAGAGCTAAC TCAAACACAT 
GAAGCTACTG TAGCAATGGA GAGAGCAGAG GTCAACAAAG TCACAAATGA GAACAAGGCT 
CTTGCCAACG AGTTGGATGA ACTTCAACAG AGAGCCAATG AGATGAGCTC AAAGGTCGAA 
ACGATCCAAA CTGAATTGGG CAATCAGTCA AAGAAGGCCA ATGAGCTTTC AGAAGAACTC 
CATACTGCCA GGGCTCTGAT CAGGGAACAT CAAGAAGAGT CTTGCAGTCA CTCCCAAACT 
ATTGAATCAT TAATGATAGA GGCTGCTCAT TTGCGTCTAG AACTCAGTAC ATGCCAGCTG 
TCACGTGATA GAAAGGAAGC TGAATGTGAA AGAATGGTCG CGTTATCTCA GGAGAAAAAC 
AGACAGATCA GTGACCTGGA GCAGGAGCTC AAGCAAACCA GAGAAAGTGT GTGTCAACTA 
GAGGAACTCT GTAGTCGGCT TAAAAGTGAG CGTGATGCCC AGGCTCAAGA GTACCAGAGC 
CTTCTAAACC GTTACGAAGC ACAAAATTTA GTTGTGACTC AAATAAGAAG CAACTCTGAT 
CTTTTGGAGG AGCTGACTGC TCTAAAACAT CAGCACAAGA AAATTGAAAA ACAAGATGAT 
TGCTGGAAAA CCGTGCAGGA CAACCTCATA CAAACAGTGA GCAAACTAAA CCAGCAAGAG 
GAGATTCTGA GCATAGTGCA AACAATTGTA ACAAATGAGA TTTCTAAGGC ACGAGATGAG 
GCAAATATGA AGATAACTGA AATGGAGAAG GATCTGTCTA ACAAAAACAC AGAGCTAGAG 
ACAAAAGCCC AAGAGCTTAT GAAAGTGAGA GAACATGTGA ACGATGGCCA CAATCAAATT 
GAGCGCATGC GCTGTGATTT GCAGCAGGTG GAGCAGGAGA GATCTGATGT CAGAGATCAG 
TTATGTGAGG CTAACACTCA GAAAGAACAT TTAGAGAAAC AGATTTTAAT CTTGAGTGAA 
GAAAGCAAAA TGTTTCAGCA GAGGCTGTGT GAGGCAGAGA AGATGAAGGA TCAGCCAAGA 
CCCTGTTTGA GCTGTATAGA GCAAACTATT GATCCACTGC AGATTGAGCA GTCTAACCAT 
GTATTAGAAA AGGAAGATAT ACAGCTTTTC CAAACACCCT GTCAAGAGAG TAAGTTGATT 
GAAGAGACTC CTGTAACCTT AAACACAGAG TTTAAACGGA CAGATGAGGA GGAATTGTTG 
AAGCCCAGAC TGACTGAGAT TGAATTCATA ACTGAAGATC TTGCAACGTT GCAGGAAAAA 
AATCAAAACC CAAGAGTTGG TGTGTCTGAT CATTCTCAGC AAGAAGATGG GAGGGCCCAG 
AGACCTGAAG AACATACCAG GCAGCTGGTC AAGAAGCAAG ACACCATTTT AAAACTGCAA 
AAGGATTCAG TTGTTTCTCC CAACTCTGAT ACCCAGGAAG CTGACAAACT CAAACAAACC 
TCAAACATGA AAACAAAGAA TGAAGGCGAC TCTGTTAGTC CAACACAAGA GGTAGCAGAA 
CAGGACTCCT CTGTACTTTC TACAAAGAGT CTCAGGGAAG AGCGTTTTCC CAAACCTGAG 
CTAGAAATCA GCTTCACGCC ACTAAAACCT CACCGAATGA ATGTCAGGAA ACCGGGCGAA 
GAAGAGTCTG TCACGATCAA ACTCCGACGT ACAGCCAGAA AGAGAAAGAG TACTGAGATA 
GAGAACTCAG TGGAGTCAGA AAACTGTAAA AACCGGAGAC TGAAGGTGAA CAACAGGGCT 
AACATGCAAT CTGTTGAGAC TCCCACAGTG CAAGGAAAAA AAATGAGTCA ATTAAAGCAA 
AAAGACTCTC CCGCTAGCCT GAAAGGTAAA AAAGACGGAC CCCTGCAGAA GATTGGCGAC 
TTTATTCAGA GCTCTCCAAC CCTCTTTGGG AGCAAAGCCA AGAAGATCAT GAGCATGGTG 
AATGTTAAAT CCCCCGAACC ACTAAATGCA TCTGAAAACA ACAAGCCCAA GAGATCCAAA 
CGCAAGCTCT TCAAGACTCA AGTTTCATCT CCGATGGACA TACCATCCTA TCCTATCATT 
GGAAGCAGTG ATGATGATAA AGAGAGCGAT CATCTCATCA TCAAGAGAAA ACTCAGAACA 
AGAACTGCCA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20bb ( XM_009306554.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304829.1 Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X4, mRNA.

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CloneID	ODa48286
Clone ID Related Accession (Same CDS sequence)	XM_009306554.2 , XM_009306554.3
Accession Version	XM_009306554.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF20B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306554.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAT CGTGTTTAAA TCACAAGCCT GACCGTGTCG ACTCTATAAC GGTTGAAGAC 
CTCCGAAAAG ATCTTTTCTC CGAGTTCTCC AAATTATCCA GGGACTCGGT GTCGCTTGAA 
AAAGAGCATT TGCAGGTCTA TCTCCGCGTA CGTCCGTTCA CCTCAACAGA GAGAGCTGAA 
GGAGAATCAC AGGATTGTAT CTCCATTGAG CCTCCAGATA CTGTGATTCT GAAGGCACCA 
AAAGCATCTC TGACTGCCAG ACACAGTGAG AGATTTGGAC CACAACTGGC ACAACGATTT 
CAGTTCTCAC AAGTGTACGG TCCCGAAACA ACTCAGAAAG AGATTTTTGA TGGAACAACC 
AAAAGTCTTG TTAAAGAAGT TTTGGACGGA GGGAATTCCT TGATTTTTAC ATATGGAGTT 
ACCAATGCTG GGAAGACATT TACTTTTCTT GGCCCTGAAT CAGATGGTGG AATTTTGCCC 
CGGTCATTAA ACGTGATTTT TAACAGCATA GAAGGCAGAA TTTATTTGCA AAATAACATC 
AAACCTCACC GATGTGTTGA CTTCACAAGA CTCACCAAAG AGCAGCAAGA TGAGGAAGCT 
ACAAATAAAA GAAACCTTCT GCGGCGATTT AAAGATAATG ACTCCCAGAA AACTTTGTCC 
AGCATGTCCA GCTCAAGTTG TTCATCGTTT GACAGCTCCA CATCTAGTGA CATGAATGGA 
GATAGTGTCT ATTTGGATGA GGACTCTCAT GTTAAATTCT CAGTGTGGGT CTCATTTTGT 
GAAATCTACA ATGAAAGCAT CCATGATCTG CTTGATTTTG TTCCAAATGG CTCTCACAGA 
AGGAATGCAT TGCGGTTAGC CCAAGATGTT AAAAGCAATT CTTTTGTTAA AGATCTAAAA 
TGGGTTCAGG TTAACAGTGC AGATGAAGCA TTAAAAGTTG TGAAAATTGG TAGGAAAAAT 
CAAAGTTTCT CATCCACCAA ACTCAACAAC ATCTCAAGCA GAAGCCACAG TATATTTTCC 
ATTCGGATGC TAAGGATTGA AGATGTGGGT GTTCCTCGAG TCCAGACAAT AAGCGAGTTG 
TCATTATGTG ATTTGGCTGG ATCTGAGAGA TGTGCAAGGA CTCAGAATAG AGGAGAACGC 
TTAAAAGAAG CTGGGAATAT CAACACCTCC CTGTTAACTC TAGGAAAATG CATCAATGCC 
CTGAGGCTCA ACCAAACTCA GTCCAAGTTT CATCAGCACA TCCCCTTCAG AGAGAGCAAG 
CTCACACATT ATTTGCAGGG GTATTTCTGT GGCCGTGGAA AAGCTTGCAT GATCATTAAC 
ATAAACCAGT GTGCTTCAGT GTATGATGAA ACTCTGAACG TTCTCAAGTT CTCAGCAGTG 
GCTCAGAAGG TAGTTGTCCT CAACCCTAAA CCAGCTCCAA GCATTGCACT CAAAAGATCT 
GCCAGAGATG TGTCCATGAT AATCAACAAT GCTGACAAGA AGGATTGGAC CAGGAGAAGC 
TCGATTATGG GCTGGGAGAT GAGCCTAGAG GACGTGCAGG AAGATGAAAA TGATGAAGAC 
ATGGATGAGG AAGAGGTGGA GAGTGACGAT GAGAGCATAG TGGACAGAAC TGTGCTAGAG 
ACTGTGGAAA ATCAAGAGCT GTGTGAGCTT CAGGTAAAAA TTGAAGAACT CCAGGAGAAG 
CTGATCAAGG AAGAGTCTGA AAAACTAACT TTGGAAGCCC GTATTCGTGA AGAAGTCACC 
ACAGAGTTTA TGGAGCTGTT TTCTAACATG GAAAAAGACT ACAGTGAACG TCTCCAAAGA 
GAGAAAGAAA TTGTTGAAGA AAGAGCAGAA AGGAGGCTGG AAATCCTGAA GAATCTGGTC 
AACAGGACAA TTGAGGAAAT GACTACAGAT GAAAATGCAG CAAAGGAAGC CAAGATGGAG 
CTTTTGGATA CCATGATCGA GGCAATGCGA AGCGATCTAA CTAAGATAAA GGGAGATGCA 
GAGTCTGTGC AGCTGTGTCT GGCCAATGTC CCTGAATCAC CTGGAAAACT CAGTCATCTG 
AGGACAGAAT TGGAGGAGAT AAAGGCGGAA CTTCTGAAAA GTCAACAACA ACTCAGTCTC 
AAATCTAAAG AGTTTGAAGA AAGCTGTATT CAGATGCAGG ATTTAAATGA CCAACTTCTC 
CAGGCAACCA GGAATTATGA AAACCAGAAA GTGAGATGTC AGGAACTCAT GTCTATCTGC 
CAGGAGAAAG ACGACATGGT ATCTAAATTA CAAACCGCTT TGGAACAAAA TGATGAGGCA 
GCTAGTAAGG ATAGGGCCCT AATTAACAAC ATCAGAGAAG AAATCCTACA TTTCAAAAAC 
AGCTGTAAGT GTAAGCTGAA TGAAGAGCAT GATGAAATGA CAAGTCAGAA AAATCAACAG 
CTTCAGCAAG AACTGAAAAT GAAAGATGAC CAGCTAAATC AACTTAAACT TGAGCATGCA 
TCACTTGAGA AAGCGATGCG AGATATCTCT GATAGATTGT CAGAGCTAAC TCAAACACAT 
GAAGCTACTG TAGCAATGGA GAGAGCAGAG GTCAACAAAG TCACAAATGA GAACAAGGCT 
CTTGCCAACG AGTTGGATGA ACTTCAACAG AGAGCCAATG AGATGAGCTC AAAGGTCGAA 
ACGATCCAAA CTGAATTGGG CAATCAGTCA AAGAAGGCCA ATGAGCTTTC AGAAGAACTC 
CATACTGCCA GGGCTCTGAT CAGGGAACAT CAAGAAGAGT CTTGCAGTCA CTCCCAAACT 
ATTGAATCAT TAATGATAGA GGCTGCTCAT TTGCGTCTAG AACTCAGTAC ATGCCAGCTG 
TCACGTGATA GAAAGGAAGC TGAATGTGAA AGAATGGTCG CGTTATCTCA GGAGAAAAAC 
AGACAGATCA GTGACCTGGA GCAGGAGCTC AAGCAAACCA GAGAAAGTGT GTGTCAACTA 
GAGGAACTCT GTAGTCGGCT TAAAAGTGAG CGTGATGCCC AGGCTCAAGA GTACCAGAGC 
CTTCTAAACC GTTACGAAGC ACAAAATTTA GTTGTGACTC AAATAAGAAG CAACTCTGAT 
CTTTTGGAGG AGCTGACTGC TCTAAAACAT CAGCACAAGA AAATTGAAAA ACAAGATGAT 
TGCTGGAAAA CCGTGCAGGA CAACCTCATA CAAACAGTGA GCAAACTAAA CCAGCAAGAG 
GAGATTCTGA GCATAGTGCA AACAATTGTA ACAAATGAGA TTTCTAAGGC ACGAGATGAG 
GCAAATATGA AGATAACTGA AATGGAGAAG GATCTGTCTA ACAAAAACAC AGAGCTAGAG 
ACAAAAGCCC AAGAGCTTAT GAAAGTGAGA GAACATGTGA ACGATGGCCA CAATCAAATT 
GAGCGCATGC GCTGTGATTT GCAGCAGGTG GAGCAGGAGA GATCTGATGT CAGAGATCAG 
TTATGTGAGG CTAACACTCA GAAAGAACAT TTAGAGAAAC AGATTTTAAT CTTGAGTGAA 
GAAAGCAAAA TGTTTCAGCA GAGGCTGTGT GAGGCAGAGA AGATGAAGGA TCAGCCAAGA 
CCCTGTTTGA GCTGTATAGA GCAAACTATT GATCCACTGC AGATTGAGCA GTCTAACCAT 
GTATTAGAAA AGGAAGATAT ACAGCTTTTC CAAACACCCT GTCAAGAGAG TAAGTTGATT 
GAAGAGACTC CTGTAACCTT AAACACAGAG TTTAAACGGA CAGATGAGGA GGAATTGTTG 
AAGCCCAGAC TGACTGAGAT TGAATTCATA ACTGAAGATC TTGCAACGTT GCAGGAAAAA 
AATCAAAACC CAAGAGTTGG TGTGTCTGAT CATTCTCAGC AAGAAGATGG GAGGGCCCAG 
AGACCTGAAG AACATACCAG GCAGCTGGTC AAGAAGCAAG ACACCATTTT AAAACTGCAA 
AAGGATTCAG TTGTTTCTCC CAACTCTGAT ACCCAGGAAG CTGACAAACT CAAACAAACC 
TCAAACATGA AAACAAAGAA TGAAGGCGAC TCTGTTAGTC CAACACAAGA GGTAGCAGAA 
CAGGACTCCT CTGTACTTTC TACAAAGAGT CTCAGGGAAG AGCGTTTTCC CAAACCTGAG 
CTAGAAATCA GCTTCACGCC ACTAAAACCT CACCGAATGA ATGTCAGGAA ACCGGGCGAA 
GAAGAGTCTG TCACGATCAA ACTCCGACGT ACAGCCAGAA AGAGAAAGAG TACTGAGATA 
GAGAACTCAG TGGAGTCAGA AAACTGTAAA AACCGGAGAC TGAAGGTGAA CAACAGGGCT 
AACATGCAAT CTGTTGAGAC TCCCACAGTG CAAGGAAAAA AAATGAGTCA ATTAAAGCAA 
AAAGACTCTC CCGCTAGCCT GAAAGGTAAA AAAGACGGAC CCCTGCAGAA GATTGGCGAC 
TTTATTCAGA GCTCTCCAAC CCTCTTTGGG AGCAAAGCCA AGAAGATCAT GAGCATGGTG 
AATGTTAAAT CCCCCGAACC ACTAAATGCA TCTGAAAACA ACAAGCCCAA GAGATCCAAA 
CGCAAGCTCT TCAAGACTCA AGTTTCATCT CCGATGGACA TACCATCCTA TCCTATCATT 
GGAAGCAGTG ATGATGATAA AGAGAGCGAT CATCTCATCA TCAAGAGAAA ACTCAGAACA 
AGAACTGCCA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304829.1
CDS	106..4743
Translation	MMESCLNHKPDRVDSITVEDLRKDLFSEFSKLSRDSVSLEKEHLQVYLRVRPFTSTERAEGESQDCISIEPPDTVILKAPKASLTARHSERFGPQLAQRFQFSQVYGPETTQKEIFDGTTKSLVKEVLDGGNSLIFTYGVTNAGKTFTFLGPESDGGILPRSLNVIFNSIEGRIYLQNNIKPHRCVDFTRLTKEQQDEEATNKRNLLRRFKDNDSQKTLSSMSSSSCSSFDSSTSSDMNGDSVYLDEDSHVKFSVWVSFCEIYNESIHDLLDFVPNGSHRRNALRLAQDVKSNSFVKDLKWVQVNSADEALKVVKIGRKNQSFSSTKLNNISSRSHSIFSIRMLRIEDVGVPRVQTISELSLCDLAGSERCARTQNRGERLKEAGNINTSLLTLGKCINALRLNQTQSKFHQHIPFRESKLTHYLQGYFCGRGKACMIININQCASVYDETLNVLKFSAVAQKVVVLNPKPAPSIALKRSARDVSMIINNADKKDWTRRSSIMGWEMSLEDVQEDENDEDMDEEEVESDDESIVDRTVLETVENQELCELQVKIEELQEKLIKEESEKLTLEARIREEVTTEFMELFSNMEKDYSERLQREKEIVEERAERRLEILKNLVNRTIEEMTTDENAAKEAKMELLDTMIEAMRSDLTKIKGDAESVQLCLANVPESPGKLSHLRTELEEIKAELLKSQQQLSLKSKEFEESCIQMQDLNDQLLQATRNYENQKVRCQELMSICQEKDDMVSKLQTALEQNDEAASKDRALINNIREEILHFKNSCKCKLNEEHDEMTSQKNQQLQQELKMKDDQLNQLKLEHASLEKAMRDISDRLSELTQTHEATVAMERAEVNKVTNENKALANELDELQQRANEMSSKVETIQTELGNQSKKANELSEELHTARALIREHQEESCSHSQTIESLMIEAAHLRLELSTCQLSRDRKEAECERMVALSQEKNRQISDLEQELKQTRESVCQLEELCSRLKSERDAQAQEYQSLLNRYEAQNLVVTQIRSNSDLLEELTALKHQHKKIEKQDDCWKTVQDNLIQTVSKLNQQEEILSIVQTIVTNEISKARDEANMKITEMEKDLSNKNTELETKAQELMKVREHVNDGHNQIERMRCDLQQVEQERSDVRDQLCEANTQKEHLEKQILILSEESKMFQQRLCEAEKMKDQPRPCLSCIEQTIDPLQIEQSNHVLEKEDIQLFQTPCQESKLIEETPVTLNTEFKRTDEEELLKPRLTEIEFITEDLATLQEKNQNPRVGVSDHSQQEDGRAQRPEEHTRQLVKKQDTILKLQKDSVVSPNSDTQEADKLKQTSNMKTKNEGDSVSPTQEVAEQDSSVLSTKSLREERFPKPELEISFTPLKPHRMNVRKPGEEESVTIKLRRTARKRKSTEIENSVESENCKNRRLKVNNRANMQSVETPTVQGKKMSQLKQKDSPASLKGKKDGPLQKIGDFIQSSPTLFGSKAKKIMSMVNVKSPEPLNASENNKPKRSKRKLFKTQVSSPMDIPSYPIIGSSDDDKESDHLIIKRKLRTRTAKR

Target ORF information:

RefSeq Version	XM_009306554.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 20Bb (kif20bb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306554.3

ORF Insert Sequence:

ATGATGGAAT CGTGTTTAAA TCACAAGCCT GACCGTGTCG ACTCTATAAC GGTTGAAGAC 
CTCCGAAAAG ATCTTTTCTC CGAGTTCTCC AAATTATCCA GGGACTCGGT GTCGCTTGAA 
AAAGAGCATT TGCAGGTCTA TCTCCGCGTA CGTCCGTTCA CCTCAACAGA GAGAGCTGAA 
GGAGAATCAC AGGATTGTAT CTCCATTGAG CCTCCAGATA CTGTGATTCT GAAGGCACCA 
AAAGCATCTC TGACTGCCAG ACACAGTGAG AGATTTGGAC CACAACTGGC ACAACGATTT 
CAGTTCTCAC AAGTGTACGG TCCCGAAACA ACTCAGAAAG AGATTTTTGA TGGAACAACC 
AAAAGTCTTG TTAAAGAAGT TTTGGACGGA GGGAATTCCT TGATTTTTAC ATATGGAGTT 
ACCAATGCTG GGAAGACATT TACTTTTCTT GGCCCTGAAT CAGATGGTGG AATTTTGCCC 
CGGTCATTAA ACGTGATTTT TAACAGCATA GAAGGCAGAA TTTATTTGCA AAATAACATC 
AAACCTCACC GATGTGTTGA CTTCACAAGA CTCACCAAAG AGCAGCAAGA TGAGGAAGCT 
ACAAATAAAA GAAACCTTCT GCGGCGATTT AAAGATAATG ACTCCCAGAA AACTTTGTCC 
AGCATGTCCA GCTCAAGTTG TTCATCGTTT GACAGCTCCA CATCTAGTGA CATGAATGGA 
GATAGTGTCT ATTTGGATGA GGACTCTCAT GTTAAATTCT CAGTGTGGGT CTCATTTTGT 
GAAATCTACA ATGAAAGCAT CCATGATCTG CTTGATTTTG TTCCAAATGG CTCTCACAGA 
AGGAATGCAT TGCGGTTAGC CCAAGATGTT AAAAGCAATT CTTTTGTTAA AGATCTAAAA 
TGGGTTCAGG TTAACAGTGC AGATGAAGCA TTAAAAGTTG TGAAAATTGG TAGGAAAAAT 
CAAAGTTTCT CATCCACCAA ACTCAACAAC ATCTCAAGCA GAAGCCACAG TATATTTTCC 
ATTCGGATGC TAAGGATTGA AGATGTGGGT GTTCCTCGAG TCCAGACAAT AAGCGAGTTG 
TCATTATGTG ATTTGGCTGG ATCTGAGAGA TGTGCAAGGA CTCAGAATAG AGGAGAACGC 
TTAAAAGAAG CTGGGAATAT CAACACCTCC CTGTTAACTC TAGGAAAATG CATCAATGCC 
CTGAGGCTCA ACCAAACTCA GTCCAAGTTT CATCAGCACA TCCCCTTCAG AGAGAGCAAG 
CTCACACATT ATTTGCAGGG GTATTTCTGT GGCCGTGGAA AAGCTTGCAT GATCATTAAC 
ATAAACCAGT GTGCTTCAGT GTATGATGAA ACTCTGAACG TTCTCAAGTT CTCAGCAGTG 
GCTCAGAAGG TAGTTGTCCT CAACCCTAAA CCAGCTCCAA GCATTGCACT CAAAAGATCT 
GCCAGAGATG TGTCCATGAT AATCAACAAT GCTGACAAGA AGGATTGGAC CAGGAGAAGC 
TCGATTATGG GCTGGGAGAT GAGCCTAGAG GACGTGCAGG AAGATGAAAA TGATGAAGAC 
ATGGATGAGG AAGAGGTGGA GAGTGACGAT GAGAGCATAG TGGACAGAAC TGTGCTAGAG 
ACTGTGGAAA ATCAAGAGCT GTGTGAGCTT CAGGTAAAAA TTGAAGAACT CCAGGAGAAG 
CTGATCAAGG AAGAGTCTGA AAAACTAACT TTGGAAGCCC GTATTCGTGA AGAAGTCACC 
ACAGAGTTTA TGGAGCTGTT TTCTAACATG GAAAAAGACT ACAGTGAACG TCTCCAAAGA 
GAGAAAGAAA TTGTTGAAGA AAGAGCAGAA AGGAGGCTGG AAATCCTGAA GAATCTGGTC 
AACAGGACAA TTGAGGAAAT GACTACAGAT GAAAATGCAG CAAAGGAAGC CAAGATGGAG 
CTTTTGGATA CCATGATCGA GGCAATGCGA AGCGATCTAA CTAAGATAAA GGGAGATGCA 
GAGTCTGTGC AGCTGTGTCT GGCCAATGTC CCTGAATCAC CTGGAAAACT CAGTCATCTG 
AGGACAGAAT TGGAGGAGAT AAAGGCGGAA CTTCTGAAAA GTCAACAACA ACTCAGTCTC 
AAATCTAAAG AGTTTGAAGA AAGCTGTATT CAGATGCAGG ATTTAAATGA CCAACTTCTC 
CAGGCAACCA GGAATTATGA AAACCAGAAA GTGAGATGTC AGGAACTCAT GTCTATCTGC 
CAGGAGAAAG ACGACATGGT ATCTAAATTA CAAACCGCTT TGGAACAAAA TGATGAGGCA 
GCTAGTAAGG ATAGGGCCCT AATTAACAAC ATCAGAGAAG AAATCCTACA TTTCAAAAAC 
AGCTGTAAGT GTAAGCTGAA TGAAGAGCAT GATGAAATGA CAAGTCAGAA AAATCAACAG 
CTTCAGCAAG AACTGAAAAT GAAAGATGAC CAGCTAAATC AACTTAAACT TGAGCATGCA 
TCACTTGAGA AAGCGATGCG AGATATCTCT GATAGATTGT CAGAGCTAAC TCAAACACAT 
GAAGCTACTG TAGCAATGGA GAGAGCAGAG GTCAACAAAG TCACAAATGA GAACAAGGCT 
CTTGCCAACG AGTTGGATGA ACTTCAACAG AGAGCCAATG AGATGAGCTC AAAGGTCGAA 
ACGATCCAAA CTGAATTGGG CAATCAGTCA AAGAAGGCCA ATGAGCTTTC AGAAGAACTC 
CATACTGCCA GGGCTCTGAT CAGGGAACAT CAAGAAGAGT CTTGCAGTCA CTCCCAAACT 
ATTGAATCAT TAATGATAGA GGCTGCTCAT TTGCGTCTAG AACTCAGTAC ATGCCAGCTG 
TCACGTGATA GAAAGGAAGC TGAATGTGAA AGAATGGTCG CGTTATCTCA GGAGAAAAAC 
AGACAGATCA GTGACCTGGA GCAGGAGCTC AAGCAAACCA GAGAAAGTGT GTGTCAACTA 
GAGGAACTCT GTAGTCGGCT TAAAAGTGAG CGTGATGCCC AGGCTCAAGA GTACCAGAGC 
CTTCTAAACC GTTACGAAGC ACAAAATTTA GTTGTGACTC AAATAAGAAG CAACTCTGAT 
CTTTTGGAGG AGCTGACTGC TCTAAAACAT CAGCACAAGA AAATTGAAAA ACAAGATGAT 
TGCTGGAAAA CCGTGCAGGA CAACCTCATA CAAACAGTGA GCAAACTAAA CCAGCAAGAG 
GAGATTCTGA GCATAGTGCA AACAATTGTA ACAAATGAGA TTTCTAAGGC ACGAGATGAG 
GCAAATATGA AGATAACTGA AATGGAGAAG GATCTGTCTA ACAAAAACAC AGAGCTAGAG 
ACAAAAGCCC AAGAGCTTAT GAAAGTGAGA GAACATGTGA ACGATGGCCA CAATCAAATT 
GAGCGCATGC GCTGTGATTT GCAGCAGGTG GAGCAGGAGA GATCTGATGT CAGAGATCAG 
TTATGTGAGG CTAACACTCA GAAAGAACAT TTAGAGAAAC AGATTTTAAT CTTGAGTGAA 
GAAAGCAAAA TGTTTCAGCA GAGGCTGTGT GAGGCAGAGA AGATGAAGGA TCAGCCAAGA 
CCCTGTTTGA GCTGTATAGA GCAAACTATT GATCCACTGC AGATTGAGCA GTCTAACCAT 
GTATTAGAAA AGGAAGATAT ACAGCTTTTC CAAACACCCT GTCAAGAGAG TAAGTTGATT 
GAAGAGACTC CTGTAACCTT AAACACAGAG TTTAAACGGA CAGATGAGGA GGAATTGTTG 
AAGCCCAGAC TGACTGAGAT TGAATTCATA ACTGAAGATC TTGCAACGTT GCAGGAAAAA 
AATCAAAACC CAAGAGTTGG TGTGTCTGAT CATTCTCAGC AAGAAGATGG GAGGGCCCAG 
AGACCTGAAG AACATACCAG GCAGCTGGTC AAGAAGCAAG ACACCATTTT AAAACTGCAA 
AAGGATTCAG TTGTTTCTCC CAACTCTGAT ACCCAGGAAG CTGACAAACT CAAACAAACC 
TCAAACATGA AAACAAAGAA TGAAGGCGAC TCTGTTAGTC CAACACAAGA GGTAGCAGAA 
CAGGACTCCT CTGTACTTTC TACAAAGAGT CTCAGGGAAG AGCGTTTTCC CAAACCTGAG 
CTAGAAATCA GCTTCACGCC ACTAAAACCT CACCGAATGA ATGTCAGGAA ACCGGGCGAA 
GAAGAGTCTG TCACGATCAA ACTCCGACGT ACAGCCAGAA AGAGAAAGAG TACTGAGATA 
GAGAACTCAG TGGAGTCAGA AAACTGTAAA AACCGGAGAC TGAAGGTGAA CAACAGGGCT 
AACATGCAAT CTGTTGAGAC TCCCACAGTG CAAGGAAAAA AAATGAGTCA ATTAAAGCAA 
AAAGACTCTC CCGCTAGCCT GAAAGGTAAA AAAGACGGAC CCCTGCAGAA GATTGGCGAC 
TTTATTCAGA GCTCTCCAAC CCTCTTTGGG AGCAAAGCCA AGAAGATCAT GAGCATGGTG 
AATGTTAAAT CCCCCGAACC ACTAAATGCA TCTGAAAACA ACAAGCCCAA GAGATCCAAA 
CGCAAGCTCT TCAAGACTCA AGTTTCATCT CCGATGGACA TACCATCCTA TCCTATCATT 
GGAAGCAGTG ATGATGATAA AGAGAGCGAT CATCTCATCA TCAAGAGAAA ACTCAGAACA 
AGAACTGCCA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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kif20bb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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