KIF20B cDNA ORF clone, Bos taurus(cattle)

The following KIF20B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIF20B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa238950 NM_001105258.2
Latest version!
Bos taurus kinesin family member 20B (KIF20B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa230853 XM_005225362.4
Latest version!
Bos taurus kinesin family member 20B (KIF20B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa230854 XM_010819733.3
Latest version!
Bos taurus kinesin family member 20B (KIF20B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa230855 XM_005225363.4
Latest version!
Bos taurus kinesin family member 20B (KIF20B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa230856 XM_024985611.1
Latest version!
Bos taurus kinesin family member 20B (KIF20B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa37254 NM_001105258.1
Latest version!
Bos taurus kinesin family member 20B (KIF20B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa33439 XM_005225362.3
Latest version!
Bos taurus kinesin family member 20B (KIF20B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa33440 XM_005225363.3
Latest version!
Bos taurus kinesin family member 20B (KIF20B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa49054 XM_010819733.2
Latest version!
Bos taurus kinesin family member 20B (KIF20B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa238950
    Clone ID Related Accession (Same CDS sequence) NM_001105258.2
    Accession Version NM_001105258.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF20B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000026.1. On May 29, 2018 this sequence version replaced NM_001105258.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5571259.36078.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145458, SAMN04019640 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
    CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA
    TTTTCCTTAG TTGCTTCAAG CACTGAGGCA AACAGTTCTG AATCTAAAGA TTATCTCCAA
    GTTTGTCTTC GAGTAAGACC ATTCACACAG CCAGAAAAAG AACATGAGTC TGAGGGCTGT
    GTGTATATTC TGGATTCACA GACTGTTGTC CTGAAGGATC CTCAGTATAC TACCCTTGGT
    CGTTTAAGTG AGAAAAGCTC TGGGCAGATG GCACAAAAAT TCAGTTTTTC CAAGGTTTTT
    GGCCCAGAAA CTACACAGAA GGAATTCTTC CAGGGTTGCA TTCTGCAGCC AGTTAAAGAC
    CTCTTGAAAG GACAGAGTCG GCTGATTTTT ACTTACGGGC TAACCAATTC TGGAAAAACT
    TACACGTTTC AAGGCACAGA AGAAAATACT GGCATTCTTC CTCGAACTTT GAATGTATTA
    TTTGATAGTC TTCAGGAAAG ACTGTATACA AAGATGAACC TTAAACCACA TAGGTCCAGA
    GAATACTTAC GGTTATCACC AGACCAAGAG AAAGAAGAAG TCGCTAGCAA AAGTGCATTA
    CTTCGGCAAA TTAAAGAGGT TGTTATGCAT AATGACAGTT ATGGTGCTCT TTATGGAAGT
    CTAACAAACT CTTTGAGTAT CCCAGAGTTT GAAGAATCCA TGAAAGATTG TGAACACTCA
    AACTTGAACA TAGATATTAA TAGAAAATTT TCTGTGTGGG TATCTTTCTT TGAGATTTAC
    AACGAATGTA TTTACGATTT GTTTGTTCCT GTATCATCTA AGTTCCAAAA GAGAAAGACA
    CTGCGCCTTT CCCAAGATGT AAAGGGCTAT TCTTTCATAA AAGATCTACA GTGGATTCAA
    GTATCTGATG CCAAAGAAGC GTATAGACTT TTAAAGCTAG GAACAAAGCA CCAGAGTGTT
    GCTTTCACAA AACTGAACAA TGCTTCTAGT AGAAGTCACA GCATATTTAC CATTAGAATA
    TTACAGATTG AAGATTCAGA AATACCACAT GTAATACGAG TCAGTGAATT GTCTTTATGT
    GATCTTGCTG GCTCAGAACG AAGCATGAGG ACACAGAATG AAGGTGAAAG GTTAAGAGAG
    ACCGGGAATA TCAACACTTC TTTATTGACT CTGGGAAAGT GTATCAGTGT TTTGAAGAAT
    AGCGAAAAGT CAAAGTTTCA ACAGCATGTG CCTTTCCGGG AAAGTAAATT GACCCACTAT
    TTTCAAAGTT TTTTTAATGG TAAAGGGAAG ATATGTATGA TTGTCAACAT TAGCCAATGT
    TCTTTTACCT ATGATGAGAC ACTCAATGTG TTGAAGTTCT CAGCCATTGC ACAAAAAGTT
    TATGTTCCAG ATATTTTAAA TTGCTCTCAA GAGAAATCAT TTGGACCTGT CAAATCTTCT
    CAAGATGAAT CATTAGATAT TAATAATTCA GATAATAAAA TACTAAATGA AAAAAGATCC
    ACAATTTCAT GGGAAAGTAG CCTGGAAGAT GTGGTGGAAG ATGAAGATTT GGTTGAGGAT
    CTAGAAAAAG CTGAAGAAAA GCAGAATGTG GAAACTGAAT TTATTGATGA AGATCTACAT
    GATACATTAG AGGATGATAA GGCTTTTAGT AGCCATGCAG GGAAGAGAAA ACTTCTGGAT
    TTAATAGAAG ATTTGAAAAA AAAACTAATA AATGAAAGAA AGGAAAAGTT AACATTGGAA
    TTTAAAATTC GTGACGAAGT TACACAGGAG TTTACTCAGT ATTTGGCCCA ACGAGAAGCT
    GACTTTAAGG AAACTCTCCT TCAGGAACGA GAAATTTTAG AAGAAAATGC TGAATGTCGT
    TTGGCTATTT TCAAGGATTT GATTGGTAAA TGTGATGCTC AGGAGGAACC AAAGAATGAA
    GATCATGCCA TAAAAGATGA AACTGAAGAA GCTATTGCTT GTTTAGAAAT AAAGTTTAAT
    CAAGTTAAAG CTGAATTAGC TAAAACCAAA GAAGAATTAA TCAAAACCCG AGAAGAGTTA
    AAAAAGAGAG AAAGTGAATC AGAAATTAAT TTAAATTCAT TGGTTCAAGA GCTTGAGAAA
    TCTAATAAGA AAATAATTAT GCAGAATCAA AGAATTCAAG AGTTGATGGA TAAAATTGAC
    CAAAAAGAGG ATACTATTAA CAAATTTCAG AACCTAAAAT TTCATATGGA AAACACATGT
    AAAGGCAGTG ACAATGTTGG TAATTCTTCT TTAATAATAA ACAGTAAATT GGTTTGTGAT
    GAAACGACTG AAATGTCTAA GGACAGCAAA ACTAAAACGT ATTCAGGAAG AAAAAGATTA
    AATGAAAACG AACTTCAACA AGATGAACCA CCAGCAAAGA AAGGACTTAC GCATGCTAGT
    CCAGCTATCA CTGAAGACCA AAAGAAAAGT GAAGAAATGC AAGAGAGCAT TTCAGATGAG
    GAAAACATTA GACTTCTACA AGAAAATAAT GAAGAGCTAA AAACACGTTT ACTCACCATT
    GAGAATGAAC TTAAAAATGA AAAGGAAGAA AAAACAGAAT TAAATAAACA GATTGTTAGT
    TTGCAGCAGG AACTTTCTTC TTCTGAAAAA AAAAGTTTAG GCTTTAGTAT AGAGATCCAA
    CAAATTCAGT CGAGTTATGA CAATGTGATT TCTGAATTAC ATGTGCAGAA AAGTATAAAT
    CAAGAACAGA AGGAAAGGAT CATGAAATTG TCAGAGGAGA TAGAAACTGC TAGACGAAAC
    ATCACAAACA ATGTTTCACA AATAAAATTA ATGCAAGCAA AAATAGATGA ACTGCGTAGA
    CTTGATTCAG TTTCTCAGAT CGCAAACATA GATTTACTCA ATCTCAGGGA TCTGTCCAGT
    TGTTCTCAAG AAGATAATTT GCCAAACACA CAGTTACATC ATTTGGATAA TGATTGTTTG
    ATAAGTAAGC AGGTTAAAGA ACATGGTATT CAAGAACTTA GTAGGGAAAG GTCTTTCCAC
    TCTACTGTTG AAGCCATTTG GGAAGAATGT AAAGAGATTG TAAAGACTTC CTCCAAAAAA
    ACCCATCAGA TTGAGGAACT AGAACAACAA ATTGAAAAAT TACAGGCAGA AGTAAAAGAC
    TATACAAATG AAAACAATAG ACTAAAAATA GAGGAGAGGG AGAAAAAGAA TCAAGATGAT
    TTACTAAAAG AAAAAGATAG TCTTATACAG CAGCTGAAGG AAGAAGTGCA AGAAAAAACC
    ATTAGTCTTG ATATTGAAGT ACAGCATGTA GTGGAAGGAA AAAGAGCACT TTCAGAACTT
    ATGCAAGATG TTACTTGCTA TAAGGTGAAA ATAAAGGAAC TTGAAGCAAT GTTAGAAACT
    CAGAAAGATG AATGTAGCCA TTCAGCCAAG TTAGAACAAG AAATTATGGA AAAGGAATCT
    ATCATTTTAA AGCAAGAAAG AAATCTGAAT GAATGTCAAG CAAATCTTAA AGATTCTATC
    CAAAATGCCA GAGATTTAAG TGAAAGGGAG GTCAAATTGA AAGAAGAAAT CATGCAATTA
    ACAAAAAATT TGCAAGATGC TAAGCAATCA CTTCAATTAA AGGAAGAAGA AAAGGAAAGA
    AACTGGCAAG AAACAGAAAA GTTGAAAGAG GAGCTCTCTG CAAGCACTAC TCTTACCCAG
    AATCTGAAAG CTGATCTTCA GAGGAAGGAA GAAGATTATG CTGAGCTCAA AGAGAAACTG
    GCTGATGCCA AAAAGCAGAT TGAGCAAGTA CAGAAAGAGA TCTCTGTAAT GCGTGATGAG
    GAGAAATTGT TGAGGATTAA AATTAATGAA CTGGAGAAAA AGAAAAACCA GTGTTCTCAG
    GAAATAGATA TGAAACAGCG AACCATTCAG CAACTTAAGG AGCAGTTAAA TAATCAAAAA
    GTGGAAGAAG CTATACAACA GTATGAGAGA GTGTGCAAAG ATCTGAGTGT TAAAGAAAAA
    ATAATCAAAG ACATGCAAAT GACCCTGGAA GAACAAGAGC AGACTCAGGT AGAACAAGAT
    CAAGTGCTTG AGGCTAAATT AGAGGAAACC GAAAGACTGG CCACAGAATT GGAAGAATGG
    AAGGAAAAAT ACAAAGATTT GGAAGCCAAA CACAATCAAA GTTCAAATAA AGAATTTGAG
    GATAACACAG ATGTACTTAA TACAAAACTC AGTAAGCTTC AAGATGAGTT ACAGGAATCG
    GAACAGAAAC ATGAAGCTGA AAGAAAAAAA TGGTTAGAAG AAAAAATGAT GCTTATCACT
    CAAGCAAAAG AAGCTGAGAA TCTACGAAAC AAAGAGATGA AAAAATATGC CGAAGACAGA
    GAGCATTGTT TGAAGCAACA AAATGAAATG GAAATACTTA AAGGACAGTT GGCTGAGAAA
    GATGGTAACC TCCAGCAGTG GCGGGAAGAA CGCGATCAAC TTGTTGCAGC TTTAGAAATA
    CAGCTGAAAG CACTGATCTC CAGTAATGTA CAAAAAGATA ATGAAATCGA ACAATTAAAA
    AAGATCACAT CAGAGGCTTC TAAGACAGAA AAACAGACCA TGGATATCCA CCTCACACAA
    ATGAGTTTAA CAGATCCTGG CAGGGTTGAA ATTGAACCTC AGTCAACAAG TTTTGAAATT
    TCCAGAGGTG AAGTAGAGGA TGGATCTGTA GTCCTTGACT CTTGTGAAGT GTCGACAGAA
    AATAATCAAA GCACTCGATT TCCAAAACCT GAGTTAGAGA TTCAATTTAC ACCTTTACAG
    CCAAACAGAA TGGCAGTGAA ACACCCAGGT TGTACCTCAC CAGTGACGGT TAAGATTCCC
    AAGGCCCGAA AGAGGAAAAG TCATGAAATG GAAGAGGACT TTGTGAAATT TGAAAATAAG
    AAGAATGCTA CACCAAGAAT TAATTTGAAG TCCCCTGTTT CTGAGCATAG AAATTCTTTC
    AAAAAGGAAC AAAAGGTTTC CATACGCCCA TCATCTAAGA AAACTTATTC TTTACGGAGT
    CATGCATCCA CAGTTGGTGT AAACGGGTCT GCTAAGAAAA AAGAAGGAAC GCTACAGAAA
    TTTGGAGACT TCTTACAACA TTCTCCAACT ATTCTTCAGT CAAAAGCAAA GAAGATAATT
    GAAACAATGA GCTCTTCAAA GTTCTCAAAT GTAGAAGTAA GTAAAGAAAA TGTGTCTCGA
    CCAAAACGGG CCAAACGAAA ATTATACACA AATGAAATTT CATCTCCTAT TGATATATCT
    GGCCAAGTGA TTTTGATGGA CCGTAAAGTG AAAGAAAGCG ATCATCAGAT TCTCAAACGA
    CGACTTCGAA CCAGAACAGT CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001098728.1
    CDS1..5367
    Translation

    Target ORF information:

    RefSeq Version NM_001105258.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 20B (KIF20B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001105258.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
    CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA
    TTTTCCTTAG TTGCTTCAAG CACTGAGGCA AACAGTTCTG AATCTAAAGA TTATCTCCAA
    GTTTGTCTTC GAGTAAGACC ATTCACACAG CCAGAAAAAG AACATGAGTC TGAGGGCTGT
    GTGTATATTC TGGATTCACA GACTGTTGTC CTGAAGGATC CTCAGTATAC TACCCTTGGT
    CGTTTAAGTG AGAAAAGCTC TGGGCAGATG GCACAAAAAT TCAGTTTTTC CAAGGTTTTT
    GGCCCAGAAA CTACACAGAA GGAATTCTTC CAGGGTTGCA TTCTGCAGCC AGTTAAAGAC
    CTCTTGAAAG GACAGAGTCG GCTGATTTTT ACTTACGGGC TAACCAATTC TGGAAAAACT
    TACACGTTTC AAGGCACAGA AGAAAATACT GGCATTCTTC CTCGAACTTT GAATGTATTA
    TTTGATAGTC TTCAGGAAAG ACTGTATACA AAGATGAACC TTAAACCACA TAGGTCCAGA
    GAATACTTAC GGTTATCACC AGACCAAGAG AAAGAAGAAG TCGCTAGCAA AAGTGCATTA
    CTTCGGCAAA TTAAAGAGGT TGTTATGCAT AATGACAGTT ATGGTGCTCT TTATGGAAGT
    CTAACAAACT CTTTGAGTAT CCCAGAGTTT GAAGAATCCA TGAAAGATTG TGAACACTCA
    AACTTGAACA TAGATATTAA TAGAAAATTT TCTGTGTGGG TATCTTTCTT TGAGATTTAC
    AACGAATGTA TTTACGATTT GTTTGTTCCT GTATCATCTA AGTTCCAAAA GAGAAAGACA
    CTGCGCCTTT CCCAAGATGT AAAGGGCTAT TCTTTCATAA AAGATCTACA GTGGATTCAA
    GTATCTGATG CCAAAGAAGC GTATAGACTT TTAAAGCTAG GAACAAAGCA CCAGAGTGTT
    GCTTTCACAA AACTGAACAA TGCTTCTAGT AGAAGTCACA GCATATTTAC CATTAGAATA
    TTACAGATTG AAGATTCAGA AATACCACAT GTAATACGAG TCAGTGAATT GTCTTTATGT
    GATCTTGCTG GCTCAGAACG AAGCATGAGG ACACAGAATG AAGGTGAAAG GTTAAGAGAG
    ACCGGGAATA TCAACACTTC TTTATTGACT CTGGGAAAGT GTATCAGTGT TTTGAAGAAT
    AGCGAAAAGT CAAAGTTTCA ACAGCATGTG CCTTTCCGGG AAAGTAAATT GACCCACTAT
    TTTCAAAGTT TTTTTAATGG TAAAGGGAAG ATATGTATGA TTGTCAACAT TAGCCAATGT
    TCTTTTACCT ATGATGAGAC ACTCAATGTG TTGAAGTTCT CAGCCATTGC ACAAAAAGTT
    TATGTTCCAG ATATTTTAAA TTGCTCTCAA GAGAAATCAT TTGGACCTGT CAAATCTTCT
    CAAGATGAAT CATTAGATAT TAATAATTCA GATAATAAAA TACTAAATGA AAAAAGATCC
    ACAATTTCAT GGGAAAGTAG CCTGGAAGAT GTGGTGGAAG ATGAAGATTT GGTTGAGGAT
    CTAGAAAAAG CTGAAGAAAA GCAGAATGTG GAAACTGAAT TTATTGATGA AGATCTACAT
    GATACATTAG AGGATGATAA GGCTTTTAGT AGCCATGCAG GGAAGAGAAA ACTTCTGGAT
    TTAATAGAAG ATTTGAAAAA AAAACTAATA AATGAAAGAA AGGAAAAGTT AACATTGGAA
    TTTAAAATTC GTGACGAAGT TACACAGGAG TTTACTCAGT ATTTGGCCCA ACGAGAAGCT
    GACTTTAAGG AAACTCTCCT TCAGGAACGA GAAATTTTAG AAGAAAATGC TGAATGTCGT
    TTGGCTATTT TCAAGGATTT GATTGGTAAA TGTGATGCTC AGGAGGAACC AAAGAATGAA
    GATCATGCCA TAAAAGATGA AACTGAAGAA GCTATTGCTT GTTTAGAAAT AAAGTTTAAT
    CAAGTTAAAG CTGAATTAGC TAAAACCAAA GAAGAATTAA TCAAAACCCG AGAAGAGTTA
    AAAAAGAGAG AAAGTGAATC AGAAATTAAT TTAAATTCAT TGGTTCAAGA GCTTGAGAAA
    TCTAATAAGA AAATAATTAT GCAGAATCAA AGAATTCAAG AGTTGATGGA TAAAATTGAC
    CAAAAAGAGG ATACTATTAA CAAATTTCAG AACCTAAAAT TTCATATGGA AAACACATGT
    AAAGGCAGTG ACAATGTTGG TAATTCTTCT TTAATAATAA ACAGTAAATT GGTTTGTGAT
    GAAACGACTG AAATGTCTAA GGACAGCAAA ACTAAAACGT ATTCAGGAAG AAAAAGATTA
    AATGAAAACG AACTTCAACA AGATGAACCA CCAGCAAAGA AAGGACTTAC GCATGCTAGT
    CCAGCTATCA CTGAAGACCA AAAGAAAAGT GAAGAAATGC AAGAGAGCAT TTCAGATGAG
    GAAAACATTA GACTTCTACA AGAAAATAAT GAAGAGCTAA AAACACGTTT ACTCACCATT
    GAGAATGAAC TTAAAAATGA AAAGGAAGAA AAAACAGAAT TAAATAAACA GATTGTTAGT
    TTGCAGCAGG AACTTTCTTC TTCTGAAAAA AAAAGTTTAG GCTTTAGTAT AGAGATCCAA
    CAAATTCAGT CGAGTTATGA CAATGTGATT TCTGAATTAC ATGTGCAGAA AAGTATAAAT
    CAAGAACAGA AGGAAAGGAT CATGAAATTG TCAGAGGAGA TAGAAACTGC TAGACGAAAC
    ATCACAAACA ATGTTTCACA AATAAAATTA ATGCAAGCAA AAATAGATGA ACTGCGTAGA
    CTTGATTCAG TTTCTCAGAT CGCAAACATA GATTTACTCA ATCTCAGGGA TCTGTCCAGT
    TGTTCTCAAG AAGATAATTT GCCAAACACA CAGTTACATC ATTTGGATAA TGATTGTTTG
    ATAAGTAAGC AGGTTAAAGA ACATGGTATT CAAGAACTTA GTAGGGAAAG GTCTTTCCAC
    TCTACTGTTG AAGCCATTTG GGAAGAATGT AAAGAGATTG TAAAGACTTC CTCCAAAAAA
    ACCCATCAGA TTGAGGAACT AGAACAACAA ATTGAAAAAT TACAGGCAGA AGTAAAAGAC
    TATACAAATG AAAACAATAG ACTAAAAATA GAGGAGAGGG AGAAAAAGAA TCAAGATGAT
    TTACTAAAAG AAAAAGATAG TCTTATACAG CAGCTGAAGG AAGAAGTGCA AGAAAAAACC
    ATTAGTCTTG ATATTGAAGT ACAGCATGTA GTGGAAGGAA AAAGAGCACT TTCAGAACTT
    ATGCAAGATG TTACTTGCTA TAAGGTGAAA ATAAAGGAAC TTGAAGCAAT GTTAGAAACT
    CAGAAAGATG AATGTAGCCA TTCAGCCAAG TTAGAACAAG AAATTATGGA AAAGGAATCT
    ATCATTTTAA AGCAAGAAAG AAATCTGAAT GAATGTCAAG CAAATCTTAA AGATTCTATC
    CAAAATGCCA GAGATTTAAG TGAAAGGGAG GTCAAATTGA AAGAAGAAAT CATGCAATTA
    ACAAAAAATT TGCAAGATGC TAAGCAATCA CTTCAATTAA AGGAAGAAGA AAAGGAAAGA
    AACTGGCAAG AAACAGAAAA GTTGAAAGAG GAGCTCTCTG CAAGCACTAC TCTTACCCAG
    AATCTGAAAG CTGATCTTCA GAGGAAGGAA GAAGATTATG CTGAGCTCAA AGAGAAACTG
    GCTGATGCCA AAAAGCAGAT TGAGCAAGTA CAGAAAGAGA TCTCTGTAAT GCGTGATGAG
    GAGAAATTGT TGAGGATTAA AATTAATGAA CTGGAGAAAA AGAAAAACCA GTGTTCTCAG
    GAAATAGATA TGAAACAGCG AACCATTCAG CAACTTAAGG AGCAGTTAAA TAATCAAAAA
    GTGGAAGAAG CTATACAACA GTATGAGAGA GTGTGCAAAG ATCTGAGTGT TAAAGAAAAA
    ATAATCAAAG ACATGCAAAT GACCCTGGAA GAACAAGAGC AGACTCAGGT AGAACAAGAT
    CAAGTGCTTG AGGCTAAATT AGAGGAAACC GAAAGACTGG CCACAGAATT GGAAGAATGG
    AAGGAAAAAT ACAAAGATTT GGAAGCCAAA CACAATCAAA GTTCAAATAA AGAATTTGAG
    GATAACACAG ATGTACTTAA TACAAAACTC AGTAAGCTTC AAGATGAGTT ACAGGAATCG
    GAACAGAAAC ATGAAGCTGA AAGAAAAAAA TGGTTAGAAG AAAAAATGAT GCTTATCACT
    CAAGCAAAAG AAGCTGAGAA TCTACGAAAC AAAGAGATGA AAAAATATGC CGAAGACAGA
    GAGCATTGTT TGAAGCAACA AAATGAAATG GAAATACTTA AAGGACAGTT GGCTGAGAAA
    GATGGTAACC TCCAGCAGTG GCGGGAAGAA CGCGATCAAC TTGTTGCAGC TTTAGAAATA
    CAGCTGAAAG CACTGATCTC CAGTAATGTA CAAAAAGATA ATGAAATCGA ACAATTAAAA
    AAGATCACAT CAGAGGCTTC TAAGACAGAA AAACAGACCA TGGATATCCA CCTCACACAA
    ATGAGTTTAA CAGATCCTGG CAGGGTTGAA ATTGAACCTC AGTCAACAAG TTTTGAAATT
    TCCAGAGGTG AAGTAGAGGA TGGATCTGTA GTCCTTGACT CTTGTGAAGT GTCGACAGAA
    AATAATCAAA GCACTCGATT TCCAAAACCT GAGTTAGAGA TTCAATTTAC ACCTTTACAG
    CCAAACAGAA TGGCAGTGAA ACACCCAGGT TGTACCTCAC CAGTGACGGT TAAGATTCCC
    AAGGCCCGAA AGAGGAAAAG TCATGAAATG GAAGAGGACT TTGTGAAATT TGAAAATAAG
    AAGAATGCTA CACCAAGAAT TAATTTGAAG TCCCCTGTTT CTGAGCATAG AAATTCTTTC
    AAAAAGGAAC AAAAGGTTTC CATACGCCCA TCATCTAAGA AAACTTATTC TTTACGGAGT
    CATGCATCCA CAGTTGGTGT AAACGGGTCT GCTAAGAAAA AAGAAGGAAC GCTACAGAAA
    TTTGGAGACT TCTTACAACA TTCTCCAACT ATTCTTCAGT CAAAAGCAAA GAAGATAATT
    GAAACAATGA GCTCTTCAAA GTTCTCAAAT GTAGAAGTAA GTAAAGAAAA TGTGTCTCGA
    CCAAAACGGG CCAAACGAAA ATTATACACA AATGAAATTT CATCTCCTAT TGATATATCT
    GGCCAAGTGA TTTTGATGGA CCGTAAAGTG AAAGAAAGCG ATCATCAGAT TCTCAAACGA
    CGACTTCGAA CCAGAACAGT CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230853
    Clone ID Related Accession (Same CDS sequence) XM_005225362.4
    Accession Version XM_005225362.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF20B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225362.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGGGACT CCGTACATAC CAGAATGGAA TCTAATTTAA ATCAAGATGG GATGTCTAGA 
    CCATCTTATG TTTTCAGTGC TGACCCACTT GCAAGACCTT CAGAAATAAA TTTTGATGGC
    ATTAAGCTTG ACCTCTCTCG TGAATTTTCC TTAGTTGCTT CAAGCACTGA GGCAAACAGT
    TCTGAATCTA AAGATTATCT CCAAGTTTGT CTTCGAGTAA GACCATTCAC ACAGCCAGAA
    AAAGAACATG AGTCTGAGGG CTGTGTGTAT ATTCTGGATT CACAGACTGT TGTCCTGAAG
    GATCCTCAGT ATACTACCCT TGGTCGTTTA AGTGAGAAAA GCTCTGGGCA GATGGCACAA
    AAATTCAGTT TTTCCAAGGT TTTTGGCCCA GAAACTACAC AGAAGGAATT CTTCCAGGGT
    TGCATTCTGC AGCCAGTTAA AGACCTCTTG AAAGGACAGA GTCGGCTGAT TTTTACTTAC
    GGGCTAACCA ATTCTGGAAA AACTTACACG TTTCAAGGCA CAGAAGAAAA TACTGGCATT
    CTTCCTCGAA CTTTGAATGT ATTATTTGAT AGTCTTCAGG AAAGACTGTA TACAAAGATG
    AACCTTAAAC CACATAGGTC CAGAGAATAC TTACGGTTAT CACCAGACCA AGAGAAAGAA
    GAAGTCGCTA GCAAAAGTGC ATTACTTCGG CAAATTAAAG AGGTTGTTAT GCATAATGAC
    AGTTATGGTG CTCTTTATGG AAGTCTAACA AACTCTTTGA GTATCCCAGA GTTTGAAGAA
    TCCATGAAAG ATTGTGAACA CTCAAACTTG AACATAGATA TTAATAGAAA ATTTTCTGTG
    TGGGTATCTT TCTTTGAGAT TTACAACGAA TGTATTTACG ATTTGTTTGT TCCTGTATCA
    TCTAAGTTCC AAAAGAGAAA GACACTGCGC CTTTCCCAAG ATGTAAAGGG CTATTCTTTC
    ATAAAAGATC TACAGTGGAT TCAAGTATCT GATGCCAAAG AAGCGTATAG ACTTTTAAAG
    CTAGGAACAA AGCACCAGAG TGTTGCTTTC ACAAAACTGA ACAATGCTTC TAGTAGAAGT
    CACAGCATAT TTACCATTAG AATATTACAG ATTGAAGATT CAGAAATACC ACATGTAATA
    CGAGTCAGTG AATTGTCTTT ATGTGATCTT GCTGGCTCAG AACGAAGCAT GAGGACACAG
    AATGAAGGTG AAAGGTTAAG AGAGACCGGG AATATCAACA CTTCTTTATT GACTCTGGGA
    AAGTGTATCA GTGTTTTGAA GAATAGCGAA AAGTCAAAGT TTCAACAGCA TGTGCCTTTC
    CGGGAAAGTA AATTGACCCA CTATTTTCAA AGTTTTTTTA ATGGTAAAGG GAAGATATGT
    ATGATTGTCA ACATTAGCCA ATGTTCTTTT ACCTATGATG AGACACTCAA TGTGTTGAAG
    TTCTCAGCCA TTGCACAAAA AGTTTATGTT CCAGATATTT TAAATTGCTC TCAAGAGAAA
    TCATTTGGAC CTGTCAAATC TTCTCAAGAT GAATCATTAG ATATTAATAA TTCAGATAAT
    AAAATACTAA ATGAAAAAAG ATCCACAATT TCATGGGAAA GTAGCCTGGA AGATGTGGTG
    GAAGATGAAG ATTTGGTTGA GGATCTAGAA AAAGCTGAAG AAAAGCAGAA TGTGGAAACT
    GAATTTATTG ATGAAGATCT ACATGATACA TTAGAGGATG ATAAGGCTTT TAGTAGCCAT
    GCAGGGAAGA GAAAACTTCT GGATTTAATA GAAGATTTGA AAAAAAAACT AATAAATGAA
    AGAAAGGAAA AGTTAACATT GGAATTTAAA ATTCGTGACG AAGTTACACA GGAGTTTACT
    CAGTATTTGG CCCAACGAGA AGCTGACTTT AAGGAAACTC TCCTTCAGGA ACGAGAAATT
    TTAGAAGAAA ATGCTGAATG TCGTTTGGCT ATTTTCAAGG ATTTGATTGG TAAATGTGAT
    GCTCAGGAGG AACCAAAGAA TGAAGATCAT GCCATAAAAG ATGAAACTGA AGAAGGACAT
    AATTATGTAG GAATTGAAGA TATTATTGAT TCTCTTCAAG ATGATGTCAC TGGTATTAAG
    AAACAGGCTG AAATTGCTCA CTTATATATT GCATCTCTTG CTGACCCCCA GGAAGCTATT
    GCTTGTTTAG AAATAAAGTT TAATCAAGTT AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA
    TTAATCAAAA CCCGAGAAGA GTTAAAAAAG AGAGAAAGTG AATCAGAAAT TAATTTAAAT
    TCATTGGTTC AAGAGCTTGA GAAATCTAAT AAGAAAATAA TTATGCAGAA TCAAAGAATT
    CAAGAGTTGA TGGATAAAAT TGACCAAAAA GAGGATACTA TTAACAAATT TCAGAACCTA
    AAATTTCATA TGGAAAACAC ATGTAAAGGC AGTGACAATG TTGGTAATTC TTCTTTAATA
    ATAAACAGTA AATTGGTTTG TGATGAAACG ACTGAAATGT CTAAGGACAG CAAAACTAAA
    ACGTATTCAG GAAGAAAAAG ATTAAATGAA AACGAACTTC AACAAGATGA ACCACCAGCA
    AAGAAAGGAC TTACGCATGC TAGTCCAGCT ATCACTGAAG ACCAAAAGAA AAGTGAAGAA
    ATGCAAGAGA GCATTTCAGA TGAGGAAAAC ATTAGACTTC TACAAGAAAA TAATGAAGAG
    CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA
    GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT
    TTAGGCTTTA GTATAGAGAT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA
    TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATGAA ATTGTCAGAG
    GAGATAGAAA CTGCTAGACG AAACATCACA AACAATGTTT CACAAATAAA ATTAATGCAA
    GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTTTCTC AGATCGCAAA CATAGATTTA
    CTCAATCTCA GGGATCTGTC CAGTTGTTCT CAAGAAGATA ATTTGCCAAA CACACAGTTA
    CATCATTTGG ATAATGATTG TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA
    CTTAGTAGGG AAAGGTCTTT CCACTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG
    ATTGTAAAGA CTTCCTCCAA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA
    AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ATAGACTAAA AATAGAGGAG
    AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG
    AAGGAAGAAG TGCAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA
    GGAAAAAGAG CACTTTCAGA ACTTATGCAA GATGTTACTT GCTATAAGGT GAAAATAAAG
    GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA
    CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT
    CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAGGTCAAA
    TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA ATCACTTCAA
    TTAAAGGAAG AAGAAAAGGA AAGAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC
    TCTGCAAGCA CTACTCTTAC CCAGAATCTG AAAGCTGATC TTCAGAGGAA GGAAGAAGAT
    TATGCTGAGC TCAAAGAGAA ACTGGCTGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA
    GAGATCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG
    AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT
    AAGGAGCAGT TAAATAATCA AAAAGTGGAA GAAGCTATAC AACAGTATGA GAGAGTGTGC
    AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA
    GAGCAGACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA
    CTGGCCACAG AATTGGAAGA ATGGAAGGAA AAATACAAAG ATTTGGAAGC CAAACACAAT
    CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCAGTAAG
    CTTCAAGATG AGTTACAGGA ATCGGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA
    GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAATCTACG AAACAAAGAG
    ATGAAAAAAT ATGCCGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGA AATGGAAATA
    CTTAAAGGAC AGTTGGCTGA GAAAGATGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT
    CAACTTGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA
    GATAATGAAA TCGAACAATT AAAAAAGATC ACATCAGAGG CTTCTAAGAC AGAAAAACAG
    ACCATGGATA TCCACCTCAC ACAAATGAGT TTAACAGATC CTGGCAGGGT TGAAATTGAA
    CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT
    GACTCTTGTG AAGTGTCGAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA
    GAGATTCAAT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC
    TCACCAGTGA CGGTTAAGAT TCCCAAGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG
    GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT
    GTTTCTGAGC ATAGAAATTC TTTCAAAAAG GAACAAAAGG TTTCCATACG CCCATCATCT
    AAGAAAACTT ATTCTTTACG GAGTCATGCA TCCACAGTTG GTGTAAACGG GTCTGCTAAG
    AAAAAAGAAG GAACGCTACA GAAATTTGGA GACTTCTTAC AACATTCTCC AACTATTCTT
    CAGTCAAAAG CAAAGAAGAT AATTGAAACA ATGAGCTCTT CAAAGTTCTC AAATGTAGAA
    GTAAGTAAAG AAAATGTGTC TCGACCAAAA CGGGCCAAAC GAAAATTATA CACAAATGAA
    ATTTCATCTC CTATTGATAT ATCTGGCCAA GTGATTTTGA TGGACCGTAA AGTGAAAGAA
    AGCGATCATC AGATTCTCAA ACGACGACTT CGAACCAGAA CAGTCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225419.1
    CDS132..5642
    Translation

    Target ORF information:

    RefSeq Version XM_005225362.4
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 20B (KIF20B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225362.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGGGACT CCGTACATAC CAGAATGGAA TCTAATTTAA ATCAAGATGG GATGTCTAGA 
    CCATCTTATG TTTTCAGTGC TGACCCACTT GCAAGACCTT CAGAAATAAA TTTTGATGGC
    ATTAAGCTTG ACCTCTCTCG TGAATTTTCC TTAGTTGCTT CAAGCACTGA GGCAAACAGT
    TCTGAATCTA AAGATTATCT CCAAGTTTGT CTTCGAGTAA GACCATTCAC ACAGCCAGAA
    AAAGAACATG AGTCTGAGGG CTGTGTGTAT ATTCTGGATT CACAGACTGT TGTCCTGAAG
    GATCCTCAGT ATACTACCCT TGGTCGTTTA AGTGAGAAAA GCTCTGGGCA GATGGCACAA
    AAATTCAGTT TTTCCAAGGT TTTTGGCCCA GAAACTACAC AGAAGGAATT CTTCCAGGGT
    TGCATTCTGC AGCCAGTTAA AGACCTCTTG AAAGGACAGA GTCGGCTGAT TTTTACTTAC
    GGGCTAACCA ATTCTGGAAA AACTTACACG TTTCAAGGCA CAGAAGAAAA TACTGGCATT
    CTTCCTCGAA CTTTGAATGT ATTATTTGAT AGTCTTCAGG AAAGACTGTA TACAAAGATG
    AACCTTAAAC CACATAGGTC CAGAGAATAC TTACGGTTAT CACCAGACCA AGAGAAAGAA
    GAAGTCGCTA GCAAAAGTGC ATTACTTCGG CAAATTAAAG AGGTTGTTAT GCATAATGAC
    AGTTATGGTG CTCTTTATGG AAGTCTAACA AACTCTTTGA GTATCCCAGA GTTTGAAGAA
    TCCATGAAAG ATTGTGAACA CTCAAACTTG AACATAGATA TTAATAGAAA ATTTTCTGTG
    TGGGTATCTT TCTTTGAGAT TTACAACGAA TGTATTTACG ATTTGTTTGT TCCTGTATCA
    TCTAAGTTCC AAAAGAGAAA GACACTGCGC CTTTCCCAAG ATGTAAAGGG CTATTCTTTC
    ATAAAAGATC TACAGTGGAT TCAAGTATCT GATGCCAAAG AAGCGTATAG ACTTTTAAAG
    CTAGGAACAA AGCACCAGAG TGTTGCTTTC ACAAAACTGA ACAATGCTTC TAGTAGAAGT
    CACAGCATAT TTACCATTAG AATATTACAG ATTGAAGATT CAGAAATACC ACATGTAATA
    CGAGTCAGTG AATTGTCTTT ATGTGATCTT GCTGGCTCAG AACGAAGCAT GAGGACACAG
    AATGAAGGTG AAAGGTTAAG AGAGACCGGG AATATCAACA CTTCTTTATT GACTCTGGGA
    AAGTGTATCA GTGTTTTGAA GAATAGCGAA AAGTCAAAGT TTCAACAGCA TGTGCCTTTC
    CGGGAAAGTA AATTGACCCA CTATTTTCAA AGTTTTTTTA ATGGTAAAGG GAAGATATGT
    ATGATTGTCA ACATTAGCCA ATGTTCTTTT ACCTATGATG AGACACTCAA TGTGTTGAAG
    TTCTCAGCCA TTGCACAAAA AGTTTATGTT CCAGATATTT TAAATTGCTC TCAAGAGAAA
    TCATTTGGAC CTGTCAAATC TTCTCAAGAT GAATCATTAG ATATTAATAA TTCAGATAAT
    AAAATACTAA ATGAAAAAAG ATCCACAATT TCATGGGAAA GTAGCCTGGA AGATGTGGTG
    GAAGATGAAG ATTTGGTTGA GGATCTAGAA AAAGCTGAAG AAAAGCAGAA TGTGGAAACT
    GAATTTATTG ATGAAGATCT ACATGATACA TTAGAGGATG ATAAGGCTTT TAGTAGCCAT
    GCAGGGAAGA GAAAACTTCT GGATTTAATA GAAGATTTGA AAAAAAAACT AATAAATGAA
    AGAAAGGAAA AGTTAACATT GGAATTTAAA ATTCGTGACG AAGTTACACA GGAGTTTACT
    CAGTATTTGG CCCAACGAGA AGCTGACTTT AAGGAAACTC TCCTTCAGGA ACGAGAAATT
    TTAGAAGAAA ATGCTGAATG TCGTTTGGCT ATTTTCAAGG ATTTGATTGG TAAATGTGAT
    GCTCAGGAGG AACCAAAGAA TGAAGATCAT GCCATAAAAG ATGAAACTGA AGAAGGACAT
    AATTATGTAG GAATTGAAGA TATTATTGAT TCTCTTCAAG ATGATGTCAC TGGTATTAAG
    AAACAGGCTG AAATTGCTCA CTTATATATT GCATCTCTTG CTGACCCCCA GGAAGCTATT
    GCTTGTTTAG AAATAAAGTT TAATCAAGTT AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA
    TTAATCAAAA CCCGAGAAGA GTTAAAAAAG AGAGAAAGTG AATCAGAAAT TAATTTAAAT
    TCATTGGTTC AAGAGCTTGA GAAATCTAAT AAGAAAATAA TTATGCAGAA TCAAAGAATT
    CAAGAGTTGA TGGATAAAAT TGACCAAAAA GAGGATACTA TTAACAAATT TCAGAACCTA
    AAATTTCATA TGGAAAACAC ATGTAAAGGC AGTGACAATG TTGGTAATTC TTCTTTAATA
    ATAAACAGTA AATTGGTTTG TGATGAAACG ACTGAAATGT CTAAGGACAG CAAAACTAAA
    ACGTATTCAG GAAGAAAAAG ATTAAATGAA AACGAACTTC AACAAGATGA ACCACCAGCA
    AAGAAAGGAC TTACGCATGC TAGTCCAGCT ATCACTGAAG ACCAAAAGAA AAGTGAAGAA
    ATGCAAGAGA GCATTTCAGA TGAGGAAAAC ATTAGACTTC TACAAGAAAA TAATGAAGAG
    CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA
    GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT
    TTAGGCTTTA GTATAGAGAT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA
    TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATGAA ATTGTCAGAG
    GAGATAGAAA CTGCTAGACG AAACATCACA AACAATGTTT CACAAATAAA ATTAATGCAA
    GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTTTCTC AGATCGCAAA CATAGATTTA
    CTCAATCTCA GGGATCTGTC CAGTTGTTCT CAAGAAGATA ATTTGCCAAA CACACAGTTA
    CATCATTTGG ATAATGATTG TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA
    CTTAGTAGGG AAAGGTCTTT CCACTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG
    ATTGTAAAGA CTTCCTCCAA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA
    AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ATAGACTAAA AATAGAGGAG
    AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG
    AAGGAAGAAG TGCAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA
    GGAAAAAGAG CACTTTCAGA ACTTATGCAA GATGTTACTT GCTATAAGGT GAAAATAAAG
    GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA
    CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT
    CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAGGTCAAA
    TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA ATCACTTCAA
    TTAAAGGAAG AAGAAAAGGA AAGAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC
    TCTGCAAGCA CTACTCTTAC CCAGAATCTG AAAGCTGATC TTCAGAGGAA GGAAGAAGAT
    TATGCTGAGC TCAAAGAGAA ACTGGCTGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA
    GAGATCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG
    AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT
    AAGGAGCAGT TAAATAATCA AAAAGTGGAA GAAGCTATAC AACAGTATGA GAGAGTGTGC
    AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA
    GAGCAGACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA
    CTGGCCACAG AATTGGAAGA ATGGAAGGAA AAATACAAAG ATTTGGAAGC CAAACACAAT
    CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCAGTAAG
    CTTCAAGATG AGTTACAGGA ATCGGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA
    GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAATCTACG AAACAAAGAG
    ATGAAAAAAT ATGCCGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGA AATGGAAATA
    CTTAAAGGAC AGTTGGCTGA GAAAGATGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT
    CAACTTGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA
    GATAATGAAA TCGAACAATT AAAAAAGATC ACATCAGAGG CTTCTAAGAC AGAAAAACAG
    ACCATGGATA TCCACCTCAC ACAAATGAGT TTAACAGATC CTGGCAGGGT TGAAATTGAA
    CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT
    GACTCTTGTG AAGTGTCGAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA
    GAGATTCAAT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC
    TCACCAGTGA CGGTTAAGAT TCCCAAGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG
    GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT
    GTTTCTGAGC ATAGAAATTC TTTCAAAAAG GAACAAAAGG TTTCCATACG CCCATCATCT
    AAGAAAACTT ATTCTTTACG GAGTCATGCA TCCACAGTTG GTGTAAACGG GTCTGCTAAG
    AAAAAAGAAG GAACGCTACA GAAATTTGGA GACTTCTTAC AACATTCTCC AACTATTCTT
    CAGTCAAAAG CAAAGAAGAT AATTGAAACA ATGAGCTCTT CAAAGTTCTC AAATGTAGAA
    GTAAGTAAAG AAAATGTGTC TCGACCAAAA CGGGCCAAAC GAAAATTATA CACAAATGAA
    ATTTCATCTC CTATTGATAT ATCTGGCCAA GTGATTTTGA TGGACCGTAA AGTGAAAGAA
    AGCGATCATC AGATTCTCAA ACGACGACTT CGAACCAGAA CAGTCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230854
    Clone ID Related Accession (Same CDS sequence) XM_010819733.3
    Accession Version XM_010819733.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF20B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819733.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
    CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA
    TTTTCCTTAG TTGCTTCAAG CACTGAGGCA AACAGTTCTG AATCTAAAGA TTATCTCCAA
    GTTTGTCTTC GAGTAAGACC ATTCACACAG CCAGAAAAAG AACATGAGTC TGAGGGCTGT
    GTGTATATTC TGGATTCACA GACTGTTGTC CTGAAGGATC CTCAGTATAC TACCCTTGGT
    CGTTTAAGTG AGAAAAGCTC TGGGCAGATG GCACAAAAAT TCAGTTTTTC CAAGGTTTTT
    GGCCCAGAAA CTACACAGAA GGAATTCTTC CAGGGTTGCA TTCTGCAGCC AGTTAAAGAC
    CTCTTGAAAG GACAGAGTCG GCTGATTTTT ACTTACGGGC TAACCAATTC TGGAAAAACT
    TACACGTTTC AAGGCACAGA AGAAAATACT GGCATTCTTC CTCGAACTTT GAATGTATTA
    TTTGATAGTC TTCAGGAAAG ACTGTATACA AAGATGAACC TTAAACCACA TAGGTCCAGA
    GAATACTTAC GGTTATCACC AGACCAAGAG AAAGAAGAAG TCGCTAGCAA AAGTGCATTA
    CTTCGGCAAA TTAAAGAGGT TGTTATGCAT AATGACAGTT ATGGTGCTCT TTATGGAAGT
    CTAACAAACT CTTTGAGTAT CCCAGAGTTT GAAGAATCCA TGAAAGATTG TGAACACTCA
    AACTTGAACA TAGATATTAA TAGAAAATTT TCTGTGTGGG TATCTTTCTT TGAGATTTAC
    AACGAATGTA TTTACGATTT GTTTGTTCCT GTATCATCTA AGTTCCAAAA GAGAAAGACA
    CTGCGCCTTT CCCAAGATGT AAAGGGCTAT TCTTTCATAA AAGATCTACA GTGGATTCAA
    GTATCTGATG CCAAAGAAGC GTATAGACTT TTAAAGCTAG GAACAAAGCA CCAGAGTGTT
    GCTTTCACAA AACTGAACAA TGCTTCTAGT AGAAGTCACA GCATATTTAC CATTAGAATA
    TTACAGATTG AAGATTCAGA AATACCACAT GTAATACGAG TCAGTGAATT GTCTTTATGT
    GATCTTGCTG GCTCAGAACG AAGCATGAGG ACACAGAATG AAGGTGAAAG GTTAAGAGAG
    ACCGGGAATA TCAACACTTC TTTATTGACT CTGGGAAAGT GTATCAGTGT TTTGAAGAAT
    AGCGAAAAGT CAAAGTTTCA ACAGCATGTG CCTTTCCGGG AAAGTAAATT GACCCACTAT
    TTTCAAAGTT TTTTTAATGG TAAAGGGAAG ATATGTATGA TTGTCAACAT TAGCCAATGT
    TCTTTTACCT ATGATGAGAC ACTCAATGTG TTGAAGTTCT CAGCCATTGC ACAAAAAGTT
    TATGTTCCAG ATATTTTAAA TTGCTCTCAA GAGAAATCAT TTGGACCTGT CAAATCTTCT
    CAAGATGAAT CATTAGATAT TAATAATTCA GATAATAAAA TACTAAATGA AAAAAGATCC
    ACAATTTCAT GGGAAAGTAG CCTGGAAGAT GTGGTGGAAG ATGAAGATTT GGTTGAGGAT
    CTAGAAAAAG CTGAAGAAAA GCAGAATGTG GAAACTGAAT TTATTGATGA AGATCTACAT
    GATACATTAG AGGATGATAA GGCTTTTAGT AGCCATGCAG GGAAGAGAAA ACTTCTGGAT
    TTAATAGAAG ATTTGAAAAA AAAACTAATA AATGAAAGAA AGGAAAAGTT AACATTGGAA
    TTTAAAATTC GTGACGAAGT TACACAGGAG TTTACTCAGT ATTTGGCCCA ACGAGAAGCT
    GACTTTAAGG AAACTCTCCT TCAGGAACGA GAAATTTTAG AAGAAAATGC TGAATGTCGT
    TTGGCTATTT TCAAGGATTT GATTGGTAAA TGTGATGCTC AGGAGGAACC AAAGAATGAA
    GATCATGCCA TAAAAGATGA AACTGAAGAA GGACATAATT ATGTAGGAAT TGAAGATATT
    ATTGATTCTC TTCAAGATGA TGTCACTGGT ATTAAGAAAC AGGCTGAAAT TGCTCACTTA
    TATATTGCAT CTCTTGCTGA CCCCCAGGAA GCTATTGCTT GTTTAGAAAT AAAGTTTAAT
    CAAGTTAAAG CTGAATTAGC TAAAACCAAA GAAGAATTAA TCAAAACCCG AGAAGAGTTA
    AAAAAGAGAG AAAGTGAATC AGAAATTAAT TTAAATTCAT TGGTTCAAGA GCTTGAGAAA
    TCTAATAAGA AAATAATTAT GCAGAATCAA AGAATTCAAG AGTTGATGGA TAAAATTGAC
    CAAAAAGAGG ATACTATTAA CAAATTTCAG AACCTAAAAT TTCATATGGA AAACACATGT
    AAAGGCAGTG ACAATGTTGG TAATTCTTCT TTAATAATAA ACAGTAAATT GGTTTGTGAT
    GAAACGACTG AAATGTCTAA GGACAGCAAA ACTAAAACGT ATTCAGGAAG AAAAAGATTA
    AATGAAAACG AACTTCAACA AGATGAACCA CCAGCAAAGA AAGGACTTAC GCATGCTAGT
    CCAGCTATCA CTGAAGACCA AAAGAAAAGT GAAGAAATGC AAGAGAGCAT TTCAGATGAG
    GAAAACATTA GACTTCTACA AGAAAATAAT GAAGAGCTAA AAACACGTTT ACTCACCATT
    GAGAATGAAC TTAAAAATGA AAAGGAAGAA AAAACAGAAT TAAATAAACA GATTGTTAGT
    TTGCAGCAGG AACTTTCTTC TTCTGAAAAA AAAAGTTTAG GCTTTAGTAT AGAGATCCAA
    CAAATTCAGT CGAGTTATGA CAATGTGATT TCTGAATTAC ATGTGCAGAA AAGTATAAAT
    CAAGAACAGA AGGAAAGGAT CATGAAATTG TCAGAGGAGA TAGAAACTGC TAGACGAAAC
    ATCACAAACA ATGTTTCACA AATAAAATTA ATGCAAGCAA AAATAGATGA ACTGCGTAGA
    CTTGATTCAG TTTCTCAGAT CGCAAACATA GATTTACTCA ATCTCAGGGA TCTGTCCAGT
    TGTTCTCAAG AAGATAATTT GCCAAACACA CAGTTACATC ATTTGGATAA TGATTGTTTG
    ATAAGTAAGC AGGTTAAAGA ACATGGTATT CAAGAACTTA GTAGGGAAAG GTCTTTCCAC
    TCTACTGTTG AAGCCATTTG GGAAGAATGT AAAGAGATTG TAAAGACTTC CTCCAAAAAA
    ACCCATCAGA TTGAGGAACT AGAACAACAA ATTGAAAAAT TACAGGCAGA AGTAAAAGAC
    TATACAAATG AAAACAATAG ACTAAAAATA GAGGAGAGGG AGAAAAAGAA TCAAGATGAT
    TTACTAAAAG AAAAAGATAG TCTTATACAG CAGCTGAAGG AAGAAGTGCA AGAAAAAACC
    ATTAGTCTTG ATATTGAAGT ACAGCATGTA GTGGAAGGAA AAAGAGCACT TTCAGAACTT
    ATGCAAGATG TTACTTGCTA TAAGGTGAAA ATAAAGGAAC TTGAAGCAAT GTTAGAAACT
    CAGAAAGATG AATGTAGCCA TTCAGCCAAG TTAGAACAAG AAATTATGGA AAAGGAATCT
    ATCATTTTAA AGCAAGAAAG AAATCTGAAT GAATGTCAAG CAAATCTTAA AGATTCTATC
    CAAAATGCCA GAGATTTAAG TGAAAGGGAG GTCAAATTGA AAGAAGAAAT CATGCAATTA
    ACAAAAAATT TGCAAGATGC TAAGCAATCA CTTCAATTAA AGGAAGAAGA AAAGGAAAGA
    AACTGGCAAG AAACAGAAAA GTTGAAAGAG GAGCTCTCTG CAAGCACTAC TCTTACCCAG
    AATCTGAAAG CTGATCTTCA GAGGAAGGAA GAAGATTATG CTGAGCTCAA AGAGAAACTG
    GCTGATGCCA AAAAGCAGAT TGAGCAAGTA CAGAAAGAGA TCTCTGTAAT GCGTGATGAG
    GAGAAATTGT TGAGGATTAA AATTAATGAA CTGGAGAAAA AGAAAAACCA GTGTTCTCAG
    GAAATAGATA TGAAACAGCG AACCATTCAG CAACTTAAGG AGCAGTTAAA TAATCAAAAA
    GTGGAAGAAG CTATACAACA GTATGAGAGA GTGTGCAAAG ATCTGAGTGT TAAAGAAAAA
    ATAATCAAAG ACATGCAAAT GACCCTGGAA GAACAAGAGC AGACTCAGGT AGAACAAGAT
    CAAGTGCTTG AGGCTAAATT AGAGGAAACC GAAAGACTGG CCACAGAATT GGAAGAATGG
    AAGGAAAAAT ACAAAGATTT GGAAGCCAAA CACAATCAAA GTTCAAATAA AGAATTTGAG
    GATAACACAG ATGTACTTAA TACAAAACTC AGTAAGCTTC AAGATGAGTT ACAGGAATCG
    GAACAGAAAC ATGAAGCTGA AAGAAAAAAA TGGTTAGAAG AAAAAATGAT GCTTATCACT
    CAAGCAAAAG AAGCTGAGAA TCTACGAAAC AAAGAGATGA AAAAATATGC CGAAGACAGA
    GAGCATTGTT TGAAGCAACA AAATGAAATG GAAATACTTA AAGGACAGTT GGCTGAGAAA
    GATGGTAACC TCCAGCAGTG GCGGGAAGAA CGCGATCAAC TTGTTGCAGC TTTAGAAATA
    CAGCTGAAAG CACTGATCTC CAGTAATGTA CAAAAAGATA ATGAAATCGA ACAATTAAAA
    AAGATCACAT CAGAGGCTTC TAAGACAGAA AAACAGACCA TGGATATCCA CCTCACACAA
    ATGAGTTTAA CAGATCCTGG CAGGGTTGAA ATTGAACCTC AGTCAACAAG TTTTGAAATT
    TCCAGAGGTG AAGTAGAGGA TGGATCTGTA GTCCTTGACT CTTGTGAAGT GTCGACAGAA
    AATAATCAAA GCACTCGATT TCCAAAACCT GAGTTAGAGA TTCAATTTAC ACCTTTACAG
    CCAAACAGAA TGGCAGTGAA ACACCCAGGT TGTACCTCAC CAGTGACGGT TAAGATTCCC
    AAGGCCCGAA AGAGGAAAAG TCATGAAATG GAAGAGGACT TTGTGAAATT TGAAAATAAG
    AAGAATGCTA CACCAAGAAT TAATTTGAAG TCCCCTGTTT CTGAGCATAG AAATTCTTTC
    AAAAAGGAAC AAAAGGTTTC CATACGCCCA TCATCTAAGA AAACTTATTC TTTACGGAGT
    CATGCATCCA CAGTTGGTGT AAACGGGTCT GCTAAGAAAA AAGAAGGAAC GCTACAGAAA
    TTTGGAGACT TCTTACAACA TTCTCCAACT ATTCTTCAGT CAAAAGCAAA GAAGATAATT
    GAAACAATGA GCTCTTCAAA GTTCTCAAAT GTAGAAGTAA GTAAAGAAAA TGTGTCTCGA
    CCAAAACGGG CCAAACGAAA ATTATACACA AATGAAATTT CATCTCCTAT TGATATATCT
    GGCCAAGTGA TTTTGATGGA CCGTAAAGTG AAAGAAAGCG ATCATCAGAT TCTCAAACGA
    CGACTTCGAA CCAGAACAGT CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818035.1
    CDS121..5607
    Translation

    Target ORF information:

    RefSeq Version XM_010819733.3
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 20B (KIF20B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819733.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
    CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA
    TTTTCCTTAG TTGCTTCAAG CACTGAGGCA AACAGTTCTG AATCTAAAGA TTATCTCCAA
    GTTTGTCTTC GAGTAAGACC ATTCACACAG CCAGAAAAAG AACATGAGTC TGAGGGCTGT
    GTGTATATTC TGGATTCACA GACTGTTGTC CTGAAGGATC CTCAGTATAC TACCCTTGGT
    CGTTTAAGTG AGAAAAGCTC TGGGCAGATG GCACAAAAAT TCAGTTTTTC CAAGGTTTTT
    GGCCCAGAAA CTACACAGAA GGAATTCTTC CAGGGTTGCA TTCTGCAGCC AGTTAAAGAC
    CTCTTGAAAG GACAGAGTCG GCTGATTTTT ACTTACGGGC TAACCAATTC TGGAAAAACT
    TACACGTTTC AAGGCACAGA AGAAAATACT GGCATTCTTC CTCGAACTTT GAATGTATTA
    TTTGATAGTC TTCAGGAAAG ACTGTATACA AAGATGAACC TTAAACCACA TAGGTCCAGA
    GAATACTTAC GGTTATCACC AGACCAAGAG AAAGAAGAAG TCGCTAGCAA AAGTGCATTA
    CTTCGGCAAA TTAAAGAGGT TGTTATGCAT AATGACAGTT ATGGTGCTCT TTATGGAAGT
    CTAACAAACT CTTTGAGTAT CCCAGAGTTT GAAGAATCCA TGAAAGATTG TGAACACTCA
    AACTTGAACA TAGATATTAA TAGAAAATTT TCTGTGTGGG TATCTTTCTT TGAGATTTAC
    AACGAATGTA TTTACGATTT GTTTGTTCCT GTATCATCTA AGTTCCAAAA GAGAAAGACA
    CTGCGCCTTT CCCAAGATGT AAAGGGCTAT TCTTTCATAA AAGATCTACA GTGGATTCAA
    GTATCTGATG CCAAAGAAGC GTATAGACTT TTAAAGCTAG GAACAAAGCA CCAGAGTGTT
    GCTTTCACAA AACTGAACAA TGCTTCTAGT AGAAGTCACA GCATATTTAC CATTAGAATA
    TTACAGATTG AAGATTCAGA AATACCACAT GTAATACGAG TCAGTGAATT GTCTTTATGT
    GATCTTGCTG GCTCAGAACG AAGCATGAGG ACACAGAATG AAGGTGAAAG GTTAAGAGAG
    ACCGGGAATA TCAACACTTC TTTATTGACT CTGGGAAAGT GTATCAGTGT TTTGAAGAAT
    AGCGAAAAGT CAAAGTTTCA ACAGCATGTG CCTTTCCGGG AAAGTAAATT GACCCACTAT
    TTTCAAAGTT TTTTTAATGG TAAAGGGAAG ATATGTATGA TTGTCAACAT TAGCCAATGT
    TCTTTTACCT ATGATGAGAC ACTCAATGTG TTGAAGTTCT CAGCCATTGC ACAAAAAGTT
    TATGTTCCAG ATATTTTAAA TTGCTCTCAA GAGAAATCAT TTGGACCTGT CAAATCTTCT
    CAAGATGAAT CATTAGATAT TAATAATTCA GATAATAAAA TACTAAATGA AAAAAGATCC
    ACAATTTCAT GGGAAAGTAG CCTGGAAGAT GTGGTGGAAG ATGAAGATTT GGTTGAGGAT
    CTAGAAAAAG CTGAAGAAAA GCAGAATGTG GAAACTGAAT TTATTGATGA AGATCTACAT
    GATACATTAG AGGATGATAA GGCTTTTAGT AGCCATGCAG GGAAGAGAAA ACTTCTGGAT
    TTAATAGAAG ATTTGAAAAA AAAACTAATA AATGAAAGAA AGGAAAAGTT AACATTGGAA
    TTTAAAATTC GTGACGAAGT TACACAGGAG TTTACTCAGT ATTTGGCCCA ACGAGAAGCT
    GACTTTAAGG AAACTCTCCT TCAGGAACGA GAAATTTTAG AAGAAAATGC TGAATGTCGT
    TTGGCTATTT TCAAGGATTT GATTGGTAAA TGTGATGCTC AGGAGGAACC AAAGAATGAA
    GATCATGCCA TAAAAGATGA AACTGAAGAA GGACATAATT ATGTAGGAAT TGAAGATATT
    ATTGATTCTC TTCAAGATGA TGTCACTGGT ATTAAGAAAC AGGCTGAAAT TGCTCACTTA
    TATATTGCAT CTCTTGCTGA CCCCCAGGAA GCTATTGCTT GTTTAGAAAT AAAGTTTAAT
    CAAGTTAAAG CTGAATTAGC TAAAACCAAA GAAGAATTAA TCAAAACCCG AGAAGAGTTA
    AAAAAGAGAG AAAGTGAATC AGAAATTAAT TTAAATTCAT TGGTTCAAGA GCTTGAGAAA
    TCTAATAAGA AAATAATTAT GCAGAATCAA AGAATTCAAG AGTTGATGGA TAAAATTGAC
    CAAAAAGAGG ATACTATTAA CAAATTTCAG AACCTAAAAT TTCATATGGA AAACACATGT
    AAAGGCAGTG ACAATGTTGG TAATTCTTCT TTAATAATAA ACAGTAAATT GGTTTGTGAT
    GAAACGACTG AAATGTCTAA GGACAGCAAA ACTAAAACGT ATTCAGGAAG AAAAAGATTA
    AATGAAAACG AACTTCAACA AGATGAACCA CCAGCAAAGA AAGGACTTAC GCATGCTAGT
    CCAGCTATCA CTGAAGACCA AAAGAAAAGT GAAGAAATGC AAGAGAGCAT TTCAGATGAG
    GAAAACATTA GACTTCTACA AGAAAATAAT GAAGAGCTAA AAACACGTTT ACTCACCATT
    GAGAATGAAC TTAAAAATGA AAAGGAAGAA AAAACAGAAT TAAATAAACA GATTGTTAGT
    TTGCAGCAGG AACTTTCTTC TTCTGAAAAA AAAAGTTTAG GCTTTAGTAT AGAGATCCAA
    CAAATTCAGT CGAGTTATGA CAATGTGATT TCTGAATTAC ATGTGCAGAA AAGTATAAAT
    CAAGAACAGA AGGAAAGGAT CATGAAATTG TCAGAGGAGA TAGAAACTGC TAGACGAAAC
    ATCACAAACA ATGTTTCACA AATAAAATTA ATGCAAGCAA AAATAGATGA ACTGCGTAGA
    CTTGATTCAG TTTCTCAGAT CGCAAACATA GATTTACTCA ATCTCAGGGA TCTGTCCAGT
    TGTTCTCAAG AAGATAATTT GCCAAACACA CAGTTACATC ATTTGGATAA TGATTGTTTG
    ATAAGTAAGC AGGTTAAAGA ACATGGTATT CAAGAACTTA GTAGGGAAAG GTCTTTCCAC
    TCTACTGTTG AAGCCATTTG GGAAGAATGT AAAGAGATTG TAAAGACTTC CTCCAAAAAA
    ACCCATCAGA TTGAGGAACT AGAACAACAA ATTGAAAAAT TACAGGCAGA AGTAAAAGAC
    TATACAAATG AAAACAATAG ACTAAAAATA GAGGAGAGGG AGAAAAAGAA TCAAGATGAT
    TTACTAAAAG AAAAAGATAG TCTTATACAG CAGCTGAAGG AAGAAGTGCA AGAAAAAACC
    ATTAGTCTTG ATATTGAAGT ACAGCATGTA GTGGAAGGAA AAAGAGCACT TTCAGAACTT
    ATGCAAGATG TTACTTGCTA TAAGGTGAAA ATAAAGGAAC TTGAAGCAAT GTTAGAAACT
    CAGAAAGATG AATGTAGCCA TTCAGCCAAG TTAGAACAAG AAATTATGGA AAAGGAATCT
    ATCATTTTAA AGCAAGAAAG AAATCTGAAT GAATGTCAAG CAAATCTTAA AGATTCTATC
    CAAAATGCCA GAGATTTAAG TGAAAGGGAG GTCAAATTGA AAGAAGAAAT CATGCAATTA
    ACAAAAAATT TGCAAGATGC TAAGCAATCA CTTCAATTAA AGGAAGAAGA AAAGGAAAGA
    AACTGGCAAG AAACAGAAAA GTTGAAAGAG GAGCTCTCTG CAAGCACTAC TCTTACCCAG
    AATCTGAAAG CTGATCTTCA GAGGAAGGAA GAAGATTATG CTGAGCTCAA AGAGAAACTG
    GCTGATGCCA AAAAGCAGAT TGAGCAAGTA CAGAAAGAGA TCTCTGTAAT GCGTGATGAG
    GAGAAATTGT TGAGGATTAA AATTAATGAA CTGGAGAAAA AGAAAAACCA GTGTTCTCAG
    GAAATAGATA TGAAACAGCG AACCATTCAG CAACTTAAGG AGCAGTTAAA TAATCAAAAA
    GTGGAAGAAG CTATACAACA GTATGAGAGA GTGTGCAAAG ATCTGAGTGT TAAAGAAAAA
    ATAATCAAAG ACATGCAAAT GACCCTGGAA GAACAAGAGC AGACTCAGGT AGAACAAGAT
    CAAGTGCTTG AGGCTAAATT AGAGGAAACC GAAAGACTGG CCACAGAATT GGAAGAATGG
    AAGGAAAAAT ACAAAGATTT GGAAGCCAAA CACAATCAAA GTTCAAATAA AGAATTTGAG
    GATAACACAG ATGTACTTAA TACAAAACTC AGTAAGCTTC AAGATGAGTT ACAGGAATCG
    GAACAGAAAC ATGAAGCTGA AAGAAAAAAA TGGTTAGAAG AAAAAATGAT GCTTATCACT
    CAAGCAAAAG AAGCTGAGAA TCTACGAAAC AAAGAGATGA AAAAATATGC CGAAGACAGA
    GAGCATTGTT TGAAGCAACA AAATGAAATG GAAATACTTA AAGGACAGTT GGCTGAGAAA
    GATGGTAACC TCCAGCAGTG GCGGGAAGAA CGCGATCAAC TTGTTGCAGC TTTAGAAATA
    CAGCTGAAAG CACTGATCTC CAGTAATGTA CAAAAAGATA ATGAAATCGA ACAATTAAAA
    AAGATCACAT CAGAGGCTTC TAAGACAGAA AAACAGACCA TGGATATCCA CCTCACACAA
    ATGAGTTTAA CAGATCCTGG CAGGGTTGAA ATTGAACCTC AGTCAACAAG TTTTGAAATT
    TCCAGAGGTG AAGTAGAGGA TGGATCTGTA GTCCTTGACT CTTGTGAAGT GTCGACAGAA
    AATAATCAAA GCACTCGATT TCCAAAACCT GAGTTAGAGA TTCAATTTAC ACCTTTACAG
    CCAAACAGAA TGGCAGTGAA ACACCCAGGT TGTACCTCAC CAGTGACGGT TAAGATTCCC
    AAGGCCCGAA AGAGGAAAAG TCATGAAATG GAAGAGGACT TTGTGAAATT TGAAAATAAG
    AAGAATGCTA CACCAAGAAT TAATTTGAAG TCCCCTGTTT CTGAGCATAG AAATTCTTTC
    AAAAAGGAAC AAAAGGTTTC CATACGCCCA TCATCTAAGA AAACTTATTC TTTACGGAGT
    CATGCATCCA CAGTTGGTGT AAACGGGTCT GCTAAGAAAA AAGAAGGAAC GCTACAGAAA
    TTTGGAGACT TCTTACAACA TTCTCCAACT ATTCTTCAGT CAAAAGCAAA GAAGATAATT
    GAAACAATGA GCTCTTCAAA GTTCTCAAAT GTAGAAGTAA GTAAAGAAAA TGTGTCTCGA
    CCAAAACGGG CCAAACGAAA ATTATACACA AATGAAATTT CATCTCCTAT TGATATATCT
    GGCCAAGTGA TTTTGATGGA CCGTAAAGTG AAAGAAAGCG ATCATCAGAT TCTCAAACGA
    CGACTTCGAA CCAGAACAGT CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230855
    Clone ID Related Accession (Same CDS sequence) XM_005225363.4
    Accession Version XM_005225363.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF20B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225363.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGGGACT CCGTACATAC CAGAATGGAA TCTAATTTAA ATCAAGATGG GATGTCTAGA 
    CCATCTTATG TTTTCAGTGC TGACCCACTT GCAAGACCTT CAGAAATAAA TTTTGATGGC
    ATTAAGCTTG ACCTCTCTCG TGAATTTTCC TTAGTTGCTT CAAGCACTGA GGCAAACAGT
    TCTGAATCTA AAGATTATCT CCAAGTTTGT CTTCGAGTAA GACCATTCAC ACAGCCAGAA
    AAAGAACATG AGTCTGAGGG CTGTGTGTAT ATTCTGGATT CACAGACTGT TGTCCTGAAG
    GATCCTCAGT ATACTACCCT TGGTCGTTTA AGTGAGAAAA GCTCTGGGCA GATGGCACAA
    AAATTCAGTT TTTCCAAGGT TTTTGGCCCA GAAACTACAC AGAAGGAATT CTTCCAGGGT
    TGCATTCTGC AGCCAGTTAA AGACCTCTTG AAAGGACAGA GTCGGCTGAT TTTTACTTAC
    GGGCTAACCA ATTCTGGAAA AACTTACACG TTTCAAGGCA CAGAAGAAAA TACTGGCATT
    CTTCCTCGAA CTTTGAATGT ATTATTTGAT AGTCTTCAGG AAAGACTGTA TACAAAGATG
    AACCTTAAAC CACATAGGTC CAGAGAATAC TTACGGTTAT CACCAGACCA AGAGAAAGAA
    GAAGTCGCTA GCAAAAGTGC ATTACTTCGG CAAATTAAAG AGGTTGTTAT GCATAATGAC
    AGTTATGGTG CTCTTTATGG AAGTCTAACA AACTCTTTGA GTATCCCAGA GTTTGAAGAA
    TCCATGAAAG ATTGTGAACA CTCAAACTTG AACATAGATA TTAATAGAAA ATTTTCTGTG
    TGGGTATCTT TCTTTGAGAT TTACAACGAA TGTATTTACG ATTTGTTTGT TCCTGTATCA
    TCTAAGTTCC AAAAGAGAAA GACACTGCGC CTTTCCCAAG ATGTAAAGGG CTATTCTTTC
    ATAAAAGATC TACAGTGGAT TCAAGTATCT GATGCCAAAG AAGCGTATAG ACTTTTAAAG
    CTAGGAACAA AGCACCAGAG TGTTGCTTTC ACAAAACTGA ACAATGCTTC TAGTAGAAGT
    CACAGCATAT TTACCATTAG AATATTACAG ATTGAAGATT CAGAAATACC ACATGTAATA
    CGAGTCAGTG AATTGTCTTT ATGTGATCTT GCTGGCTCAG AACGAAGCAT GAGGACACAG
    AATGAAGGTG AAAGGTTAAG AGAGACCGGG AATATCAACA CTTCTTTATT GACTCTGGGA
    AAGTGTATCA GTGTTTTGAA GAATAGCGAA AAGTCAAAGT TTCAACAGCA TGTGCCTTTC
    CGGGAAAGTA AATTGACCCA CTATTTTCAA AGTTTTTTTA ATGGTAAAGG GAAGATATGT
    ATGATTGTCA ACATTAGCCA ATGTTCTTTT ACCTATGATG AGACACTCAA TGTGTTGAAG
    TTCTCAGCCA TTGCACAAAA AGTTTATGTT CCAGATATTT TAAATTGCTC TCAAGAGAAA
    TCATTTGGAC CTGTCAAATC TTCTCAAGAT GAATCATTAG ATATTAATAA TTCAGATAAT
    AAAATACTAA ATGAAAAAAG ATCCACAATT TCATGGGAAA GTAGCCTGGA AGATGTGGTG
    GAAGATGAAG ATTTGGTTGA GGATCTAGAA AAAGCTGAAG AAAAGCAGAA TGTGGAAACT
    GAATTTATTG ATGAAGATCT ACATGATACA TTAGAGGATG ATAAGGCTTT TAGTAGCCAT
    GCAGGGAAGA GAAAACTTCT GGATTTAATA GAAGATTTGA AAAAAAAACT AATAAATGAA
    AGAAAGGAAA AGTTAACATT GGAATTTAAA ATTCGTGACG AAGTTACACA GGAGTTTACT
    CAGTATTTGG CCCAACGAGA AGCTGACTTT AAGGAAACTC TCCTTCAGGA ACGAGAAATT
    TTAGAAGAAA ATGCTGAATG TCGTTTGGCT ATTTTCAAGG ATTTGATTGG TAAATGTGAT
    GCTCAGGAGG AACCAAAGAA TGAAGATCAT GCCATAAAAG ATGAAACTGA AGAAGCTATT
    GCTTGTTTAG AAATAAAGTT TAATCAAGTT AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA
    TTAATCAAAA CCCGAGAAGA GTTAAAAAAG AGAGAAAGTG AATCAGAAAT TAATTTAAAT
    TCATTGGTTC AAGAGCTTGA GAAATCTAAT AAGAAAATAA TTATGCAGAA TCAAAGAATT
    CAAGAGTTGA TGGATAAAAT TGACCAAAAA GAGGATACTA TTAACAAATT TCAGAACCTA
    AAATTTCATA TGGAAAACAC ATGTAAAGGC AGTGACAATG TTGGTAATTC TTCTTTAATA
    ATAAACAGTA AATTGGTTTG TGATGAAACG ACTGAAATGT CTAAGGACAG CAAAACTAAA
    ACGTATTCAG GAAGAAAAAG ATTAAATGAA AACGAACTTC AACAAGATGA ACCACCAGCA
    AAGAAAGGAC TTACGCATGC TAGTCCAGCT ATCACTGAAG ACCAAAAGAA AAGTGAAGAA
    ATGCAAGAGA GCATTTCAGA TGAGGAAAAC ATTAGACTTC TACAAGAAAA TAATGAAGAG
    CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA
    GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT
    TTAGGCTTTA GTATAGAGAT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA
    TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATGAA ATTGTCAGAG
    GAGATAGAAA CTGCTAGACG AAACATCACA AACAATGTTT CACAAATAAA ATTAATGCAA
    GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTTTCTC AGATCGCAAA CATAGATTTA
    CTCAATCTCA GGGATCTGTC CAGTTGTTCT CAAGAAGATA ATTTGCCAAA CACACAGTTA
    CATCATTTGG ATAATGATTG TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA
    CTTAGTAGGG AAAGGTCTTT CCACTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG
    ATTGTAAAGA CTTCCTCCAA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA
    AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ATAGACTAAA AATAGAGGAG
    AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG
    AAGGAAGAAG TGCAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA
    GGAAAAAGAG CACTTTCAGA ACTTATGCAA GATGTTACTT GCTATAAGGT GAAAATAAAG
    GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA
    CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT
    CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAGGTCAAA
    TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA ATCACTTCAA
    TTAAAGGAAG AAGAAAAGGA AAGAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC
    TCTGCAAGCA CTACTCTTAC CCAGAATCTG AAAGCTGATC TTCAGAGGAA GGAAGAAGAT
    TATGCTGAGC TCAAAGAGAA ACTGGCTGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA
    GAGATCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG
    AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT
    AAGGAGCAGT TAAATAATCA AAAAGTGGAA GAAGCTATAC AACAGTATGA GAGAGTGTGC
    AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA
    GAGCAGACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA
    CTGGCCACAG AATTGGAAGA ATGGAAGGAA AAATACAAAG ATTTGGAAGC CAAACACAAT
    CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCAGTAAG
    CTTCAAGATG AGTTACAGGA ATCGGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA
    GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAATCTACG AAACAAAGAG
    ATGAAAAAAT ATGCCGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGA AATGGAAATA
    CTTAAAGGAC AGTTGGCTGA GAAAGATGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT
    CAACTTGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA
    GATAATGAAA TCGAACAATT AAAAAAGATC ACATCAGAGG CTTCTAAGAC AGAAAAACAG
    ACCATGGATA TCCACCTCAC ACAAATGAGT TTAACAGATC CTGGCAGGGT TGAAATTGAA
    CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT
    GACTCTTGTG AAGTGTCGAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA
    GAGATTCAAT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC
    TCACCAGTGA CGGTTAAGAT TCCCAAGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG
    GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT
    GTTTCTGAGC ATAGAAATTC TTTCAAAAAG GAACAAAAGG TTTCCATACG CCCATCATCT
    AAGAAAACTT ATTCTTTACG GAGTCATGCA TCCACAGTTG GTGTAAACGG GTCTGCTAAG
    AAAAAAGAAG GAACGCTACA GAAATTTGGA GACTTCTTAC AACATTCTCC AACTATTCTT
    CAGTCAAAAG CAAAGAAGAT AATTGAAACA ATGAGCTCTT CAAAGTTCTC AAATGTAGAA
    GTAAGTAAAG AAAATGTGTC TCGACCAAAA CGGGCCAAAC GAAAATTATA CACAAATGAA
    ATTTCATCTC CTATTGATAT ATCTGGCCAA GTGATTTTGA TGGACCGTAA AGTGAAAGAA
    AGCGATCATC AGATTCTCAA ACGACGACTT CGAACCAGAA CAGTCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225420.1
    CDS132..5522
    Translation

    Target ORF information:

    RefSeq Version XM_005225363.4
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 20B (KIF20B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225363.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGGGACT CCGTACATAC CAGAATGGAA TCTAATTTAA ATCAAGATGG GATGTCTAGA 
    CCATCTTATG TTTTCAGTGC TGACCCACTT GCAAGACCTT CAGAAATAAA TTTTGATGGC
    ATTAAGCTTG ACCTCTCTCG TGAATTTTCC TTAGTTGCTT CAAGCACTGA GGCAAACAGT
    TCTGAATCTA AAGATTATCT CCAAGTTTGT CTTCGAGTAA GACCATTCAC ACAGCCAGAA
    AAAGAACATG AGTCTGAGGG CTGTGTGTAT ATTCTGGATT CACAGACTGT TGTCCTGAAG
    GATCCTCAGT ATACTACCCT TGGTCGTTTA AGTGAGAAAA GCTCTGGGCA GATGGCACAA
    AAATTCAGTT TTTCCAAGGT TTTTGGCCCA GAAACTACAC AGAAGGAATT CTTCCAGGGT
    TGCATTCTGC AGCCAGTTAA AGACCTCTTG AAAGGACAGA GTCGGCTGAT TTTTACTTAC
    GGGCTAACCA ATTCTGGAAA AACTTACACG TTTCAAGGCA CAGAAGAAAA TACTGGCATT
    CTTCCTCGAA CTTTGAATGT ATTATTTGAT AGTCTTCAGG AAAGACTGTA TACAAAGATG
    AACCTTAAAC CACATAGGTC CAGAGAATAC TTACGGTTAT CACCAGACCA AGAGAAAGAA
    GAAGTCGCTA GCAAAAGTGC ATTACTTCGG CAAATTAAAG AGGTTGTTAT GCATAATGAC
    AGTTATGGTG CTCTTTATGG AAGTCTAACA AACTCTTTGA GTATCCCAGA GTTTGAAGAA
    TCCATGAAAG ATTGTGAACA CTCAAACTTG AACATAGATA TTAATAGAAA ATTTTCTGTG
    TGGGTATCTT TCTTTGAGAT TTACAACGAA TGTATTTACG ATTTGTTTGT TCCTGTATCA
    TCTAAGTTCC AAAAGAGAAA GACACTGCGC CTTTCCCAAG ATGTAAAGGG CTATTCTTTC
    ATAAAAGATC TACAGTGGAT TCAAGTATCT GATGCCAAAG AAGCGTATAG ACTTTTAAAG
    CTAGGAACAA AGCACCAGAG TGTTGCTTTC ACAAAACTGA ACAATGCTTC TAGTAGAAGT
    CACAGCATAT TTACCATTAG AATATTACAG ATTGAAGATT CAGAAATACC ACATGTAATA
    CGAGTCAGTG AATTGTCTTT ATGTGATCTT GCTGGCTCAG AACGAAGCAT GAGGACACAG
    AATGAAGGTG AAAGGTTAAG AGAGACCGGG AATATCAACA CTTCTTTATT GACTCTGGGA
    AAGTGTATCA GTGTTTTGAA GAATAGCGAA AAGTCAAAGT TTCAACAGCA TGTGCCTTTC
    CGGGAAAGTA AATTGACCCA CTATTTTCAA AGTTTTTTTA ATGGTAAAGG GAAGATATGT
    ATGATTGTCA ACATTAGCCA ATGTTCTTTT ACCTATGATG AGACACTCAA TGTGTTGAAG
    TTCTCAGCCA TTGCACAAAA AGTTTATGTT CCAGATATTT TAAATTGCTC TCAAGAGAAA
    TCATTTGGAC CTGTCAAATC TTCTCAAGAT GAATCATTAG ATATTAATAA TTCAGATAAT
    AAAATACTAA ATGAAAAAAG ATCCACAATT TCATGGGAAA GTAGCCTGGA AGATGTGGTG
    GAAGATGAAG ATTTGGTTGA GGATCTAGAA AAAGCTGAAG AAAAGCAGAA TGTGGAAACT
    GAATTTATTG ATGAAGATCT ACATGATACA TTAGAGGATG ATAAGGCTTT TAGTAGCCAT
    GCAGGGAAGA GAAAACTTCT GGATTTAATA GAAGATTTGA AAAAAAAACT AATAAATGAA
    AGAAAGGAAA AGTTAACATT GGAATTTAAA ATTCGTGACG AAGTTACACA GGAGTTTACT
    CAGTATTTGG CCCAACGAGA AGCTGACTTT AAGGAAACTC TCCTTCAGGA ACGAGAAATT
    TTAGAAGAAA ATGCTGAATG TCGTTTGGCT ATTTTCAAGG ATTTGATTGG TAAATGTGAT
    GCTCAGGAGG AACCAAAGAA TGAAGATCAT GCCATAAAAG ATGAAACTGA AGAAGCTATT
    GCTTGTTTAG AAATAAAGTT TAATCAAGTT AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA
    TTAATCAAAA CCCGAGAAGA GTTAAAAAAG AGAGAAAGTG AATCAGAAAT TAATTTAAAT
    TCATTGGTTC AAGAGCTTGA GAAATCTAAT AAGAAAATAA TTATGCAGAA TCAAAGAATT
    CAAGAGTTGA TGGATAAAAT TGACCAAAAA GAGGATACTA TTAACAAATT TCAGAACCTA
    AAATTTCATA TGGAAAACAC ATGTAAAGGC AGTGACAATG TTGGTAATTC TTCTTTAATA
    ATAAACAGTA AATTGGTTTG TGATGAAACG ACTGAAATGT CTAAGGACAG CAAAACTAAA
    ACGTATTCAG GAAGAAAAAG ATTAAATGAA AACGAACTTC AACAAGATGA ACCACCAGCA
    AAGAAAGGAC TTACGCATGC TAGTCCAGCT ATCACTGAAG ACCAAAAGAA AAGTGAAGAA
    ATGCAAGAGA GCATTTCAGA TGAGGAAAAC ATTAGACTTC TACAAGAAAA TAATGAAGAG
    CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA
    GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT
    TTAGGCTTTA GTATAGAGAT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA
    TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATGAA ATTGTCAGAG
    GAGATAGAAA CTGCTAGACG AAACATCACA AACAATGTTT CACAAATAAA ATTAATGCAA
    GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTTTCTC AGATCGCAAA CATAGATTTA
    CTCAATCTCA GGGATCTGTC CAGTTGTTCT CAAGAAGATA ATTTGCCAAA CACACAGTTA
    CATCATTTGG ATAATGATTG TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA
    CTTAGTAGGG AAAGGTCTTT CCACTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG
    ATTGTAAAGA CTTCCTCCAA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA
    AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ATAGACTAAA AATAGAGGAG
    AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG
    AAGGAAGAAG TGCAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA
    GGAAAAAGAG CACTTTCAGA ACTTATGCAA GATGTTACTT GCTATAAGGT GAAAATAAAG
    GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA
    CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT
    CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAGGTCAAA
    TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA ATCACTTCAA
    TTAAAGGAAG AAGAAAAGGA AAGAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC
    TCTGCAAGCA CTACTCTTAC CCAGAATCTG AAAGCTGATC TTCAGAGGAA GGAAGAAGAT
    TATGCTGAGC TCAAAGAGAA ACTGGCTGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA
    GAGATCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG
    AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT
    AAGGAGCAGT TAAATAATCA AAAAGTGGAA GAAGCTATAC AACAGTATGA GAGAGTGTGC
    AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA
    GAGCAGACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA
    CTGGCCACAG AATTGGAAGA ATGGAAGGAA AAATACAAAG ATTTGGAAGC CAAACACAAT
    CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCAGTAAG
    CTTCAAGATG AGTTACAGGA ATCGGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA
    GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAATCTACG AAACAAAGAG
    ATGAAAAAAT ATGCCGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGA AATGGAAATA
    CTTAAAGGAC AGTTGGCTGA GAAAGATGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT
    CAACTTGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA
    GATAATGAAA TCGAACAATT AAAAAAGATC ACATCAGAGG CTTCTAAGAC AGAAAAACAG
    ACCATGGATA TCCACCTCAC ACAAATGAGT TTAACAGATC CTGGCAGGGT TGAAATTGAA
    CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT
    GACTCTTGTG AAGTGTCGAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA
    GAGATTCAAT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC
    TCACCAGTGA CGGTTAAGAT TCCCAAGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG
    GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT
    GTTTCTGAGC ATAGAAATTC TTTCAAAAAG GAACAAAAGG TTTCCATACG CCCATCATCT
    AAGAAAACTT ATTCTTTACG GAGTCATGCA TCCACAGTTG GTGTAAACGG GTCTGCTAAG
    AAAAAAGAAG GAACGCTACA GAAATTTGGA GACTTCTTAC AACATTCTCC AACTATTCTT
    CAGTCAAAAG CAAAGAAGAT AATTGAAACA ATGAGCTCTT CAAAGTTCTC AAATGTAGAA
    GTAAGTAAAG AAAATGTGTC TCGACCAAAA CGGGCCAAAC GAAAATTATA CACAAATGAA
    ATTTCATCTC CTATTGATAT ATCTGGCCAA GTGATTTTGA TGGACCGTAA AGTGAAAGAA
    AGCGATCATC AGATTCTCAA ACGACGACTT CGAACCAGAA CAGTCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230856
    Clone ID Related Accession (Same CDS sequence) XM_024985611.1
    Accession Version XM_024985611.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF20B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGGGACT CCGTACATAC CAGAATGGAA TCTAATTTAA ATCAAGATGG GATGTCTAGA 
    CCATCTTATG TTTTCAGTGC TGACCCACTT GCAAGACCTT CAGAAATAAA TTTTGATGGC
    ATTAAGCTTG ACCTCTCTCG TGAATTTTCC TTAGTTGCTT CAAGCACTGA GGCAAACAGT
    TCTGAATCTA AAGATTATCT CCAAGTTTGT CTTCGAGTAA GACCATTCAC ACAGCCAGAA
    AAAGAACATG AGTCTGAGGG CTGTGTGTAT ATTCTGGATT CACAGACTGT TGTCCTGAAG
    GATCCTCAGT ATACTACCCT TGGTCGTTTA AGTGAGAAAA GCTCTGGGCA GATGGCACAA
    AAATTCAGTT TTTCCAAGGT TTTTGGCCCA GAAACTACAC AGAAGGAATT CTTCCAGGGT
    TGCATTCTGC AGCCAGTTAA AGACCTCTTG AAAGGACAGA GTCGGCTGAT TTTTACTTAC
    GGGCTAACCA ATTCTGGAAA AACTTACACG TTTCAAGGCA CAGAAGAAAA TACTGGCATT
    CTTCCTCGAA CTTTGAATGT ATTATTTGAT AGTCTTCAGG AAAGACTGTA TACAAAGATG
    AACCTTAAAC CACATAGGTC CAGAGAATAC TTACGGTTAT CACCAGACCA AGAGAAAGAA
    GAAGTCGCTA GCAAAAGTGC ATTACTTCGG CAAATTAAAG AGGTTGTTAT GCATAATGAC
    AGTTATGGTG CTCTTTATGG AAGTCTAACA AACTCTTTGA GTATCCCAGA GTTTGAAGAA
    TCCATGAAAG ATTGTGAACA CTCAAACTTG AACATAGATA TTAATAGAAA ATTTTCTGTG
    TGGGTATCTT TCTTTGAGAT TTACAACGAA TGTATTTACG ATTTGTTTGT TCCTGTATCA
    TCTAAGTTCC AAAAGAGAAA GACACTGCGC CTTTCCCAAG ATGTAAAGGG CTATTCTTTC
    ATAAAAGATC TACAGTGGAT TCAAGTATCT GATGCCAAAG AAGCGTATAG ACTTTTAAAG
    CTAGGAACAA AGCACCAGAG TGTTGCTTTC ACAAAACTGA ACAATGCTTC TAGTAGAAGT
    CACAGCATAT TTACCATTAG AATATTACAG ATTGAAGATT CAGAAATACC ACATGTAATA
    CGAGTCAGTG AATTGTCTTT ATGTGATCTT GCTGGCTCAG AACGAAGCAT GAGGACACAG
    AATGAAGGTG AAAGGTTAAG AGAGACCGGG AATATCAACA CTTCTTTATT GACTCTGGGA
    AAGTGTATCA GTGTTTTGAA GAATAGCGAA AAGTCAAAGT TTCAACAGCA TGTGCCTTTC
    CGGGAAAGTA AATTGACCCA CTATTTTCAA AGTTTTTTTA ATGGTAAAGG GAAGATATGT
    ATGATTGTCA ACATTAGCCA ATGTTCTTTT ACCTATGATG AGACACTCAA TGTGTTGAAG
    TTCTCAGCCA TTGCACAAAA AGTTTATGTT CCAGATATTT TAAATTGCTC TCAAGAGAAA
    TCATTTGGAC CTGTCAAATC TTCTCAAGAT GAATCATTAG ATATTAATAA TTCAGATAAT
    AAAATACTAA ATGAAAAAAG ATCCACAATT TCATGGGAAA GTAGCCTGGA AGATGTGGTG
    GAAGATGAAG ATTTGGTTGA GGATCTAGAA AAAGCTGAAG AAAAGCAGAA TGTGGAAACT
    GAATTTATTG ATGAAGATCT ACATGATACA TTAGAGGATG ATAAGGCTTT TAGTAGCCAT
    GCAGGGAAGA GAAAACTTCT GGATTTAATA GAAGATTTGA AAAAAAAACT AATAAATGAA
    AGAAAGGAAA AGTTAACATT GGAATTTAAA ATTCGTGACG AAGTTACACA GGAGTTTACT
    CAGTATTTGG CCCAACGAGA AGCTGACTTT AAGGAAACTC TCCTTCAGGA ACGAGAAATT
    TTAGAAGAAA ATGCTGAATG TCGTTTGGCT ATTTTCAAGG ATTTGATTGG TAAATGTGAT
    GCTCAGGAGG AACCAAAGAA TGAAGATCAT GCCATAAAAG ATGAAACTGA AGAAGGACAT
    AATTATGTAG GAATTGAAGA TATTATTGAT TCTCTTCAAG ATGATGTCAC TGGTATTAAG
    AAACAGGCTG AAATTGCTCA CTTATATATT GCATCTCTTG CTGACCCCCA GGAAGCTATT
    GCTTGTTTAG AAATAAAGTT TAATCAAGTT AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA
    TTAATCAAAA CCCGAGAAGA GTTAAAAAAG AGAGAAAGTG AATCAGAAAT TAATTTAAAT
    TCATTGGTTC AAGAGCTTGA GAAATCTAAT AAGGACAATG TTGGTAATTC TTCTTTAATA
    ATAAACAGTA AATTGGTTTG TGATGAAACG ACTGAAATGT CTAAGGACAG CAAAACTAAA
    ACGTATTCAG GAAGAAAAAG ATTAAATGAA AACGAACTTC AACAAGATGA ACCACCAGCA
    AAGAAAGGAC TTACGCATGC TAGTCCAGCT ATCACTGAAG ACCAAAAGAA AAGTGAAGAA
    ATGCAAGAGA GCATTTCAGA TGAGGAAAAC ATTAGACTTC TACAAGAAAA TAATGAAGAG
    CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA
    GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT
    TTAGGCTTTA GTATAGAGAT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA
    TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATGAA ATTGTCAGAG
    GAGATAGAAA CTGCTAGACG AAACATCACA AACAATGTTT CACAAATAAA ATTAATGCAA
    GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTTTCTC AGATCGCAAA CATAGATTTA
    CTCAATCTCA GGGATCTGTC CAGTTGTTCT CAAGAAGATA ATTTGCCAAA CACACAGTTA
    CATCATTTGG ATAATGATTG TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA
    CTTAGTAGGG AAAGGTCTTT CCACTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG
    ATTGTAAAGA CTTCCTCCAA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA
    AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ATAGACTAAA AATAGAGGAG
    AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG
    AAGGAAGAAG TGCAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA
    GGAAAAAGAG CACTTTCAGA ACTTATGCAA GATGTTACTT GCTATAAGGT GAAAATAAAG
    GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA
    CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT
    CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAGGTCAAA
    TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA ATCACTTCAA
    TTAAAGGAAG AAGAAAAGGA AAGAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC
    TCTGCAAGCA CTACTCTTAC CCAGAATCTG AAAGCTGATC TTCAGAGGAA GGAAGAAGAT
    TATGCTGAGC TCAAAGAGAA ACTGGCTGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA
    GAGATCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG
    AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT
    AAGGAGCAGT TAAATAATCA AAAAGTGGAA GAAGCTATAC AACAGTATGA GAGAGTGTGC
    AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA
    GAGCAGACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA
    CTGGCCACAG AATTGGAAGA ATGGAAGGAA AAATACAAAG ATTTGGAAGC CAAACACAAT
    CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCAGTAAG
    CTTCAAGATG AGTTACAGGA ATCGGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA
    GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAATCTACG AAACAAAGAG
    ATGAAAAAAT ATGCCGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGA AATGGAAATA
    CTTAAAGGAC AGTTGGCTGA GAAAGATGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT
    CAACTTGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA
    GATAATGAAA TCGAACAATT AAAAAAGATC ACATCAGAGG CTTCTAAGAC AGAAAAACAG
    ACCATGGATA TCCACCTCAC ACAAATGAGT TTAACAGATC CTGGCAGGGT TGAAATTGAA
    CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT
    GACTCTTGTG AAGTGTCGAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA
    GAGATTCAAT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC
    TCACCAGTGA CGGTTAAGAT TCCCAAGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG
    GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT
    GTTTCTGAGC ATAGAAATTC TTTCAAAAAG GAACAAAAGG TTTCCATACG CCCATCATCT
    AAGAAAACTT ATTCTTTACG GAGTCATGCA TCCACAGTTG GTGTAAACGG GTCTGCTAAG
    AAAAAAGAAG GAACGCTACA GAAATTTGGA GACTTCTTAC AACATTCTCC AACTATTCTT
    CAGTCAAAAG CAAAGAAGAT AATTGAAACA ATGAGCTCTT CAAAGTTCTC AAATGTAGAA
    GTAAGTAAAG AAAATGTGTC TCGACCAAAA CGGGCCAAAC GAAAATTATA CACAAATGAA
    ATTTCATCTC CTATTGATAT ATCTGGCCAA GTGATTTTGA TGGACCGTAA AGTGAAAGAA
    AGCGATCATC AGATTCTCAA ACGACGACTT CGAACCAGAA CAGTCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841379.1
    CDS132..5522
    Translation

    Target ORF information:

    RefSeq Version XM_024985611.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 20B (KIF20B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985611.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGGGACT CCGTACATAC CAGAATGGAA TCTAATTTAA ATCAAGATGG GATGTCTAGA 
    CCATCTTATG TTTTCAGTGC TGACCCACTT GCAAGACCTT CAGAAATAAA TTTTGATGGC
    ATTAAGCTTG ACCTCTCTCG TGAATTTTCC TTAGTTGCTT CAAGCACTGA GGCAAACAGT
    TCTGAATCTA AAGATTATCT CCAAGTTTGT CTTCGAGTAA GACCATTCAC ACAGCCAGAA
    AAAGAACATG AGTCTGAGGG CTGTGTGTAT ATTCTGGATT CACAGACTGT TGTCCTGAAG
    GATCCTCAGT ATACTACCCT TGGTCGTTTA AGTGAGAAAA GCTCTGGGCA GATGGCACAA
    AAATTCAGTT TTTCCAAGGT TTTTGGCCCA GAAACTACAC AGAAGGAATT CTTCCAGGGT
    TGCATTCTGC AGCCAGTTAA AGACCTCTTG AAAGGACAGA GTCGGCTGAT TTTTACTTAC
    GGGCTAACCA ATTCTGGAAA AACTTACACG TTTCAAGGCA CAGAAGAAAA TACTGGCATT
    CTTCCTCGAA CTTTGAATGT ATTATTTGAT AGTCTTCAGG AAAGACTGTA TACAAAGATG
    AACCTTAAAC CACATAGGTC CAGAGAATAC TTACGGTTAT CACCAGACCA AGAGAAAGAA
    GAAGTCGCTA GCAAAAGTGC ATTACTTCGG CAAATTAAAG AGGTTGTTAT GCATAATGAC
    AGTTATGGTG CTCTTTATGG AAGTCTAACA AACTCTTTGA GTATCCCAGA GTTTGAAGAA
    TCCATGAAAG ATTGTGAACA CTCAAACTTG AACATAGATA TTAATAGAAA ATTTTCTGTG
    TGGGTATCTT TCTTTGAGAT TTACAACGAA TGTATTTACG ATTTGTTTGT TCCTGTATCA
    TCTAAGTTCC AAAAGAGAAA GACACTGCGC CTTTCCCAAG ATGTAAAGGG CTATTCTTTC
    ATAAAAGATC TACAGTGGAT TCAAGTATCT GATGCCAAAG AAGCGTATAG ACTTTTAAAG
    CTAGGAACAA AGCACCAGAG TGTTGCTTTC ACAAAACTGA ACAATGCTTC TAGTAGAAGT
    CACAGCATAT TTACCATTAG AATATTACAG ATTGAAGATT CAGAAATACC ACATGTAATA
    CGAGTCAGTG AATTGTCTTT ATGTGATCTT GCTGGCTCAG AACGAAGCAT GAGGACACAG
    AATGAAGGTG AAAGGTTAAG AGAGACCGGG AATATCAACA CTTCTTTATT GACTCTGGGA
    AAGTGTATCA GTGTTTTGAA GAATAGCGAA AAGTCAAAGT TTCAACAGCA TGTGCCTTTC
    CGGGAAAGTA AATTGACCCA CTATTTTCAA AGTTTTTTTA ATGGTAAAGG GAAGATATGT
    ATGATTGTCA ACATTAGCCA ATGTTCTTTT ACCTATGATG AGACACTCAA TGTGTTGAAG
    TTCTCAGCCA TTGCACAAAA AGTTTATGTT CCAGATATTT TAAATTGCTC TCAAGAGAAA
    TCATTTGGAC CTGTCAAATC TTCTCAAGAT GAATCATTAG ATATTAATAA TTCAGATAAT
    AAAATACTAA ATGAAAAAAG ATCCACAATT TCATGGGAAA GTAGCCTGGA AGATGTGGTG
    GAAGATGAAG ATTTGGTTGA GGATCTAGAA AAAGCTGAAG AAAAGCAGAA TGTGGAAACT
    GAATTTATTG ATGAAGATCT ACATGATACA TTAGAGGATG ATAAGGCTTT TAGTAGCCAT
    GCAGGGAAGA GAAAACTTCT GGATTTAATA GAAGATTTGA AAAAAAAACT AATAAATGAA
    AGAAAGGAAA AGTTAACATT GGAATTTAAA ATTCGTGACG AAGTTACACA GGAGTTTACT
    CAGTATTTGG CCCAACGAGA AGCTGACTTT AAGGAAACTC TCCTTCAGGA ACGAGAAATT
    TTAGAAGAAA ATGCTGAATG TCGTTTGGCT ATTTTCAAGG ATTTGATTGG TAAATGTGAT
    GCTCAGGAGG AACCAAAGAA TGAAGATCAT GCCATAAAAG ATGAAACTGA AGAAGGACAT
    AATTATGTAG GAATTGAAGA TATTATTGAT TCTCTTCAAG ATGATGTCAC TGGTATTAAG
    AAACAGGCTG AAATTGCTCA CTTATATATT GCATCTCTTG CTGACCCCCA GGAAGCTATT
    GCTTGTTTAG AAATAAAGTT TAATCAAGTT AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA
    TTAATCAAAA CCCGAGAAGA GTTAAAAAAG AGAGAAAGTG AATCAGAAAT TAATTTAAAT
    TCATTGGTTC AAGAGCTTGA GAAATCTAAT AAGGACAATG TTGGTAATTC TTCTTTAATA
    ATAAACAGTA AATTGGTTTG TGATGAAACG ACTGAAATGT CTAAGGACAG CAAAACTAAA
    ACGTATTCAG GAAGAAAAAG ATTAAATGAA AACGAACTTC AACAAGATGA ACCACCAGCA
    AAGAAAGGAC TTACGCATGC TAGTCCAGCT ATCACTGAAG ACCAAAAGAA AAGTGAAGAA
    ATGCAAGAGA GCATTTCAGA TGAGGAAAAC ATTAGACTTC TACAAGAAAA TAATGAAGAG
    CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA
    GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT
    TTAGGCTTTA GTATAGAGAT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA
    TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATGAA ATTGTCAGAG
    GAGATAGAAA CTGCTAGACG AAACATCACA AACAATGTTT CACAAATAAA ATTAATGCAA
    GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTTTCTC AGATCGCAAA CATAGATTTA
    CTCAATCTCA GGGATCTGTC CAGTTGTTCT CAAGAAGATA ATTTGCCAAA CACACAGTTA
    CATCATTTGG ATAATGATTG TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA
    CTTAGTAGGG AAAGGTCTTT CCACTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG
    ATTGTAAAGA CTTCCTCCAA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA
    AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ATAGACTAAA AATAGAGGAG
    AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG
    AAGGAAGAAG TGCAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA
    GGAAAAAGAG CACTTTCAGA ACTTATGCAA GATGTTACTT GCTATAAGGT GAAAATAAAG
    GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA
    CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT
    CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAGGTCAAA
    TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA ATCACTTCAA
    TTAAAGGAAG AAGAAAAGGA AAGAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC
    TCTGCAAGCA CTACTCTTAC CCAGAATCTG AAAGCTGATC TTCAGAGGAA GGAAGAAGAT
    TATGCTGAGC TCAAAGAGAA ACTGGCTGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA
    GAGATCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG
    AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT
    AAGGAGCAGT TAAATAATCA AAAAGTGGAA GAAGCTATAC AACAGTATGA GAGAGTGTGC
    AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA
    GAGCAGACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA
    CTGGCCACAG AATTGGAAGA ATGGAAGGAA AAATACAAAG ATTTGGAAGC CAAACACAAT
    CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCAGTAAG
    CTTCAAGATG AGTTACAGGA ATCGGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA
    GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAATCTACG AAACAAAGAG
    ATGAAAAAAT ATGCCGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGA AATGGAAATA
    CTTAAAGGAC AGTTGGCTGA GAAAGATGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT
    CAACTTGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA
    GATAATGAAA TCGAACAATT AAAAAAGATC ACATCAGAGG CTTCTAAGAC AGAAAAACAG
    ACCATGGATA TCCACCTCAC ACAAATGAGT TTAACAGATC CTGGCAGGGT TGAAATTGAA
    CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT
    GACTCTTGTG AAGTGTCGAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA
    GAGATTCAAT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC
    TCACCAGTGA CGGTTAAGAT TCCCAAGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG
    GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT
    GTTTCTGAGC ATAGAAATTC TTTCAAAAAG GAACAAAAGG TTTCCATACG CCCATCATCT
    AAGAAAACTT ATTCTTTACG GAGTCATGCA TCCACAGTTG GTGTAAACGG GTCTGCTAAG
    AAAAAAGAAG GAACGCTACA GAAATTTGGA GACTTCTTAC AACATTCTCC AACTATTCTT
    CAGTCAAAAG CAAAGAAGAT AATTGAAACA ATGAGCTCTT CAAAGTTCTC AAATGTAGAA
    GTAAGTAAAG AAAATGTGTC TCGACCAAAA CGGGCCAAAC GAAAATTATA CACAAATGAA
    ATTTCATCTC CTATTGATAT ATCTGGCCAA GTGATTTTGA TGGACCGTAA AGTGAAAGAA
    AGCGATCATC AGATTCTCAA ACGACGACTT CGAACCAGAA CAGTCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37254
    Clone ID Related Accession (Same CDS sequence) NM_001105258.1
    Accession Version NM_001105258.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF20B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02058746.1. On Sep 22, 2007 this sequence version replaced XM_868215.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403640, SAMN02403641 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
    CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA
    TTTTCCTTAG TTGCTTCAAG CACTGAGGCA AACAGTTCTG AATCTAAAGA TTATCTCCAA
    GTTTGTCTTC GAGTAAGACC ATTCACACAG CCAGAAAAAG AACATGAGTC TGAGGGCTGT
    GTGTATATTC TGGATTCACA GACTGTTGTC CTGAAGGATC CTCAGTATAC TACCCTTGGT
    CGTTTAAGTG AGAAAAGCTC TGGGCAGATG GCACAAAAAT TCAGTTTTTC CAAGGTTTTT
    GGCCCAGAAA CTACACAGAA GGAATTCTTC CAGGGTTGCA TTCTGCAGCC AGTTAAAGAC
    CTCTTGAAAG GACAGAGTCG GCTGATTTTT ACTTACGGGC TAACCAATTC TGGAAAAACT
    TACACGTTTC AAGGCACAGA AGAAAATACT GGCATTCTTC CTCGAACTTT GAATGTATTA
    TTTGATAGTC TTCAGGAAAG ACTGTATACA AAGATGAACC TTAAACCACA TAGGTCCAGA
    GAATACTTAC GGTTATCACC AGACCAAGAG AAAGAAGAAG TCGCTAGCAA AAGTGCATTA
    CTTCGGCAAA TTAAAGAGGT TGTTATGCAT AATGACAGTT ATGGTGCTCT TTATGGAAGT
    CTAACAAACT CTTTGAGTAT CCCAGAGTTT GAAGAATCCA TGAAAGATTG TGAACACTCA
    AACTTGAACA TAGATATTAA TAGAAAATTT TCTGTGTGGG TATCTTTCTT TGAGATTTAC
    AACGAATGTA TTTACGATTT GTTTGTTCCT GTATCATCTA AGTTCCAAAA GAGAAAGACA
    CTGCGCCTTT CCCAAGATGT AAAGGGCTAT TCTTTCATAA AAGATCTACA GTGGATTCAA
    GTATCTGATG CCAAAGAAGC ATATAGACTT TTAAAGCTAG GAACAAAGCA CCAGAGTGTT
    GCTTTCACAA AACTGAACAA TGCTTCTAGT AGAAGTCACA GCATATTTAC CATTAGAATA
    TTACAGATTG AAGATTCAGA AATACCACAT GTAATACGAG TCAGTGAATT GTCTTTATGT
    GATCTTGCTG GCTCAGAACG AAGCATGAGG ACACAGAATG AAGGTGAAAG GTTAAGAGAG
    ACCGGGAATA TCAACACTTC TTTATTGACT CTGGGAAAGT GTATCAGTGT TTTGAAGAAT
    AGCGAAAAGT CAAAGTTTCA ACAGCATGTG CCTTTCCGGG AAAGTAAATT GACCCACTAT
    TTTCAAAGTT TTTTTAATGG TAAAGGGAAG ATATGTATGA TTGTCAACAT TAGCCAATGT
    TCTTTTACCT ATGATGAGAC ACTCAATGTG TTGAAGTTCT CAGCCATTGC ACAAAAAGTT
    TATGTTCCAG ATATTTTAAA TTGCTCTCAA GAGAAATCAT TTGGACCTGT CAAATCTTCT
    CAAGATGAAT CATTAGATAT TAATAATTCA GATAATAAAA TACTAAATGA AAAAAGATCC
    ACAATTTCAT GGGAAAGTAG CCTGGAAGAT GTGGTGGAAG ATGAAGATTT GGTTGAGGAT
    CTAGAAAAAG CTGAAGAAAA GCAGAATGTG GAAACTGAAT TTATTGATGA AGATCTACAT
    GATACATTAG AGGATGATAA GGCTTTTAGT AGCCATGCAG GGAAGAGAAA ACTTCTGGAT
    TTAATAGAAG ATTTGAAAAA AAAACTAATA AATGAAAGAA AGGAAAAGTT AACATTGGAA
    TTTAAAATTC GTGACGAAGT TACACAGGAG TTTACTCAGT ATTTGGCCCA ACGAGAAGCT
    GACTTTAAGG AAACTCTCCT TCAGGAACGA GAAATTTTAG AAGAAAATGC TGAATGTCGT
    TTGGCTATTT TCAAGGATTT GATTGGTAAA TGTGATGCTC AGGAGGAACC AAAGAATGAA
    GATCATGCCA TAAAAGATGA AACTGAAGAA GCTATTGCTT GTTTAGAAAT AAAGTTTAAT
    CAAGTTAAAG CTGAATTAGC TAAAACCAAA GAAGAATTAA TCAAAACCCG AGAAGAGTTA
    AAAAAGAGAG AAAGTGAATC AGAAATTAAT TTAAATTCAT TGGTTCAAGA GCTTGAGAAA
    TCTAATAAGA AAATAATTAT GCAGAATCAA AGAATTCAAG AGTTGATGGA TAAAATTGAC
    CAAAAAGAGG ATACTATTAA CAAATTTCAG AACCTAAAAT TTCATATGGA AAACACATGT
    AAAGGCAGTG ACAATGTTGG TAATTCTTCT TTAATAATAA ACAGTAAATT GGTTTGTGAT
    GAAACGACTG AAATGTCTAA GGACAGCAAA ACTAAAACGT ATTCAGGAAG AAAAAGATTA
    AATGAAAACG AACTTCAACA AGATGAACCA CCAGCAAAGA AAGGACTTAC GCATGCTAGT
    CCAGCTATCA CTGAAGACCA AAAGAAAAGT GAAGAAATGC AAGAGAGCAT TTCAGATGAG
    GAAAACATTA GACTTCTACA AGAAAATAAT GAAGAGCTAA AAACACGTTT ACTCACCATT
    GAGAATGAAC TTAAAAATGA AAAGGAAGAA AAAACAGAAT TAAATAAACA GATTGTTAGT
    TTGCAGCAGG AACTTTCTTC TTCTGAAAAA AAAAGTTTAG GCTTTAGTAT AGAGATCCAA
    CAAATTCAGT CGAGTTATGA CAATGTGATT TCTGAATTAC ATGTGCAGAA AAGTATAAAT
    CAAGAACAGA AGGAAAGGAT CATGAAATTG TCAGAGGAGA TAGAAACTGC TAGACGAAAC
    ATCACAAACA ATGTTTCACA AATAAAATTA ATGCAAGCAA AAATAGATGA ACTGCGTAGA
    CTTGATTCAG TTTCTCAGAT CGCAAACATA GATTTACTCA ATCTCAGGGA TCTGTCCAGT
    TGTTCTCAAG AAGATAATTT GCCAAACACA CAGTTACATC ATTTGGATAA TGATTGTTTG
    ATAAGTAAGC AGGTTAAAGA ACATGGTATT CAAGAACTTA GTAGGGAAAG GTCTTTCCAC
    TCTACTGTTG AAGCCATTTG GGAAGAATGT AAAGAGATTG TAAAGACTTC CTCCAAAAAA
    ACCCATCAGA TTGAGGAACT AGAACAACAA ATTGAAAAAT TACAGGCAGA AGTAAAAGAC
    TATACAAATG AAAACAATAG ACTAAAAATA GAGGAGAGGG AGAAAAAGAA TCAAGATGAT
    TTACTAAAAG AAAAAGATAG TCTTATACAG CAGCTGAAGG AAGAAGTGCA AGAAAAAACC
    ATTAGTCTTG ATATTGAAGT ACAGCATGTA GTGGAAGGAA AAAGAGCACT TTCAGAACTT
    ATGCAAGATG TTACTTGCTA TAAGGTGAAA ATAAAGGAAC TTGAAGCAAT GTTAGAAACT
    CAGAAAGATG AATGTAGCCA TTCAGCCAAG TTAGAACAAG AAATTATGGA AAAGGAATCT
    ATCATTTTAA AGCAAGAAAG AAATCTGAAT GAATGTCAAG CAAATCTTAA AGATTCTATC
    CAAAATGCCA GAGATTTAAG TGAAAGGGAG GTCAAATTGA AAGAAGAAAT CATGCAATTA
    ACAAAAAATT TGCAAGATGC TAAGCAATCA CTTCAATTAA AGGAAGAAGA AAAGGAAAGA
    AACTGGCAAG AAACAGAAAA GTTGAAAGAG GAGCTCTCTG CAAGCACTAC TCTTACCCAG
    AATCTGAAAG CTGATCTTCA GAGGAAGGAA GAAGATTATG CTGAGCTCAA AGAGAAACTG
    GCTGATGCCA AAAAGCAGAT TGAGCAAGTA CAGAAAGAGA TCTCTGTAAT GCGTGATGAG
    GAGAAATTGT TGAGGATTAA AATTAATGAA CTGGAGAAAA AGAAAAACCA GTGTTCTCAG
    GAAATAGATA TGAAACAGCG AACCATTCAG CAACTTAAGG AGCAGTTAAA TAATCAAAAA
    GTGGAAGAAG CTATACAACA GTATGAGAGA GTGTGCAAAG ATCTGAGTGT TAAAGAAAAA
    ATAATCAAAG ACATGCAAAT GACCCTGGAA GAACAAGAGC AGACTCAGGT AGAACAAGAT
    CAAGTGCTTG AGGCTAAATT AGAGGAAACC GAAAGACTGG CCACAGAATT GGAAGAATGG
    AAGGAAAAAT ACAAAGATTT GGAAGCCAAA CACAATCAAA GTTCAAATAA AGAATTTGAG
    GATAACACAG ATGTACTTAA TACAAAACTC AGTAAGCTTC AAGATGAGTT ACAGGAATCG
    GAACAGAAAC ATGAAGCTGA AAGAAAAAAA TGGTTAGAAG AAAAAATGAT GCTTATCACT
    CAAGCAAAAG AAGCTGAGAA TCTACGAAAC AAAGAGATGA AAAAATATGC CGAAGACAGA
    GAGCATTGTT TGAAGCAACA AAATGAAATG GAAATACTTA AAGGACAGTT GGCTGAGAAA
    GATGGTAACC TCCAGCAGTG GCGGGAAGAA CGCGATCAAC TTGTTGCAGC TTTAGAAATA
    CAGCTGAAAG CACTGATCTC CAGTAATGTA CAAAAAGATA ATGAAATCGA ACAATTAAAA
    AAGATCACAT CAGAGGCTTC TAAGACAGAA AAACAGACCA TGGATATCCA CCTCACACAA
    ATGAGTTTAA CAGATCCTGG CAGGGTTGAA ATTGAACCTC AGTCAACAAG TTTTGAAATT
    TCCAGAGGTG AAGTAGAGGA TGGATCTGTA GTCCTTGACT CTTGTGAAGT GTCGACAGAA
    AATAATCAAA GCACTCGATT TCCAAAACCT GAGTTAGAGA TTCAATTTAC ACCTTTACAG
    CCAAACAGAA TGGCAGTGAA ACACCCAGGT TGTACCTCAC CAGTGACGGT TAAGATTCCC
    AAGGCCCGAA AGAGGAAAAG TCATGAAATG GAAGAGGACT TTGTGAAATT TGAAAATAAG
    AAGAATGCTA CACCAAGAAT TAATTTGAAG TCCCCTGTTT CTGAGCATAG AAATTCTTTC
    AAAAAGGAAC AAAAGGTTTC CATACGCCCA TCATCTAAGA AAACTTATTC TTTACGGAGT
    CATGCATCCA CAGTTGGTGT AAACGGGTCT GCTAAGAAAA AAGAAGGAAC GCTACAGAAA
    TTTGGAGACT TCTTACAACA TTCTCCAACT ATTCTTCAGT CAAAAGCAAA GAAGATAATT
    GAAACAATGA GCTCTTCAAA GTTCTCAAAT GTAGAAGTAA GTAAAGAAAA TGTGTCTCGA
    CCAAAACGGG CCAAACGAAA ATTATACACA AATGAAATTT CATCTCCTAT TGATATATCT
    GGCCAAGTGA TTTTGATGGA CCGTAAAGTG AAAGAAAGCG ATCATCAGAT TCTCAAACGA
    CGACTTCGAA CCAGAACAGT CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001098728.1
    CDS1..5367
    Translation

    Target ORF information:

    RefSeq Version NM_001105258.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 20B (KIF20B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001105258.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
    CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA
    TTTTCCTTAG TTGCTTCAAG CACTGAGGCA AACAGTTCTG AATCTAAAGA TTATCTCCAA
    GTTTGTCTTC GAGTAAGACC ATTCACACAG CCAGAAAAAG AACATGAGTC TGAGGGCTGT
    GTGTATATTC TGGATTCACA GACTGTTGTC CTGAAGGATC CTCAGTATAC TACCCTTGGT
    CGTTTAAGTG AGAAAAGCTC TGGGCAGATG GCACAAAAAT TCAGTTTTTC CAAGGTTTTT
    GGCCCAGAAA CTACACAGAA GGAATTCTTC CAGGGTTGCA TTCTGCAGCC AGTTAAAGAC
    CTCTTGAAAG GACAGAGTCG GCTGATTTTT ACTTACGGGC TAACCAATTC TGGAAAAACT
    TACACGTTTC AAGGCACAGA AGAAAATACT GGCATTCTTC CTCGAACTTT GAATGTATTA
    TTTGATAGTC TTCAGGAAAG ACTGTATACA AAGATGAACC TTAAACCACA TAGGTCCAGA
    GAATACTTAC GGTTATCACC AGACCAAGAG AAAGAAGAAG TCGCTAGCAA AAGTGCATTA
    CTTCGGCAAA TTAAAGAGGT TGTTATGCAT AATGACAGTT ATGGTGCTCT TTATGGAAGT
    CTAACAAACT CTTTGAGTAT CCCAGAGTTT GAAGAATCCA TGAAAGATTG TGAACACTCA
    AACTTGAACA TAGATATTAA TAGAAAATTT TCTGTGTGGG TATCTTTCTT TGAGATTTAC
    AACGAATGTA TTTACGATTT GTTTGTTCCT GTATCATCTA AGTTCCAAAA GAGAAAGACA
    CTGCGCCTTT CCCAAGATGT AAAGGGCTAT TCTTTCATAA AAGATCTACA GTGGATTCAA
    GTATCTGATG CCAAAGAAGC ATATAGACTT TTAAAGCTAG GAACAAAGCA CCAGAGTGTT
    GCTTTCACAA AACTGAACAA TGCTTCTAGT AGAAGTCACA GCATATTTAC CATTAGAATA
    TTACAGATTG AAGATTCAGA AATACCACAT GTAATACGAG TCAGTGAATT GTCTTTATGT
    GATCTTGCTG GCTCAGAACG AAGCATGAGG ACACAGAATG AAGGTGAAAG GTTAAGAGAG
    ACCGGGAATA TCAACACTTC TTTATTGACT CTGGGAAAGT GTATCAGTGT TTTGAAGAAT
    AGCGAAAAGT CAAAGTTTCA ACAGCATGTG CCTTTCCGGG AAAGTAAATT GACCCACTAT
    TTTCAAAGTT TTTTTAATGG TAAAGGGAAG ATATGTATGA TTGTCAACAT TAGCCAATGT
    TCTTTTACCT ATGATGAGAC ACTCAATGTG TTGAAGTTCT CAGCCATTGC ACAAAAAGTT
    TATGTTCCAG ATATTTTAAA TTGCTCTCAA GAGAAATCAT TTGGACCTGT CAAATCTTCT
    CAAGATGAAT CATTAGATAT TAATAATTCA GATAATAAAA TACTAAATGA AAAAAGATCC
    ACAATTTCAT GGGAAAGTAG CCTGGAAGAT GTGGTGGAAG ATGAAGATTT GGTTGAGGAT
    CTAGAAAAAG CTGAAGAAAA GCAGAATGTG GAAACTGAAT TTATTGATGA AGATCTACAT
    GATACATTAG AGGATGATAA GGCTTTTAGT AGCCATGCAG GGAAGAGAAA ACTTCTGGAT
    TTAATAGAAG ATTTGAAAAA AAAACTAATA AATGAAAGAA AGGAAAAGTT AACATTGGAA
    TTTAAAATTC GTGACGAAGT TACACAGGAG TTTACTCAGT ATTTGGCCCA ACGAGAAGCT
    GACTTTAAGG AAACTCTCCT TCAGGAACGA GAAATTTTAG AAGAAAATGC TGAATGTCGT
    TTGGCTATTT TCAAGGATTT GATTGGTAAA TGTGATGCTC AGGAGGAACC AAAGAATGAA
    GATCATGCCA TAAAAGATGA AACTGAAGAA GCTATTGCTT GTTTAGAAAT AAAGTTTAAT
    CAAGTTAAAG CTGAATTAGC TAAAACCAAA GAAGAATTAA TCAAAACCCG AGAAGAGTTA
    AAAAAGAGAG AAAGTGAATC AGAAATTAAT TTAAATTCAT TGGTTCAAGA GCTTGAGAAA
    TCTAATAAGA AAATAATTAT GCAGAATCAA AGAATTCAAG AGTTGATGGA TAAAATTGAC
    CAAAAAGAGG ATACTATTAA CAAATTTCAG AACCTAAAAT TTCATATGGA AAACACATGT
    AAAGGCAGTG ACAATGTTGG TAATTCTTCT TTAATAATAA ACAGTAAATT GGTTTGTGAT
    GAAACGACTG AAATGTCTAA GGACAGCAAA ACTAAAACGT ATTCAGGAAG AAAAAGATTA
    AATGAAAACG AACTTCAACA AGATGAACCA CCAGCAAAGA AAGGACTTAC GCATGCTAGT
    CCAGCTATCA CTGAAGACCA AAAGAAAAGT GAAGAAATGC AAGAGAGCAT TTCAGATGAG
    GAAAACATTA GACTTCTACA AGAAAATAAT GAAGAGCTAA AAACACGTTT ACTCACCATT
    GAGAATGAAC TTAAAAATGA AAAGGAAGAA AAAACAGAAT TAAATAAACA GATTGTTAGT
    TTGCAGCAGG AACTTTCTTC TTCTGAAAAA AAAAGTTTAG GCTTTAGTAT AGAGATCCAA
    CAAATTCAGT CGAGTTATGA CAATGTGATT TCTGAATTAC ATGTGCAGAA AAGTATAAAT
    CAAGAACAGA AGGAAAGGAT CATGAAATTG TCAGAGGAGA TAGAAACTGC TAGACGAAAC
    ATCACAAACA ATGTTTCACA AATAAAATTA ATGCAAGCAA AAATAGATGA ACTGCGTAGA
    CTTGATTCAG TTTCTCAGAT CGCAAACATA GATTTACTCA ATCTCAGGGA TCTGTCCAGT
    TGTTCTCAAG AAGATAATTT GCCAAACACA CAGTTACATC ATTTGGATAA TGATTGTTTG
    ATAAGTAAGC AGGTTAAAGA ACATGGTATT CAAGAACTTA GTAGGGAAAG GTCTTTCCAC
    TCTACTGTTG AAGCCATTTG GGAAGAATGT AAAGAGATTG TAAAGACTTC CTCCAAAAAA
    ACCCATCAGA TTGAGGAACT AGAACAACAA ATTGAAAAAT TACAGGCAGA AGTAAAAGAC
    TATACAAATG AAAACAATAG ACTAAAAATA GAGGAGAGGG AGAAAAAGAA TCAAGATGAT
    TTACTAAAAG AAAAAGATAG TCTTATACAG CAGCTGAAGG AAGAAGTGCA AGAAAAAACC
    ATTAGTCTTG ATATTGAAGT ACAGCATGTA GTGGAAGGAA AAAGAGCACT TTCAGAACTT
    ATGCAAGATG TTACTTGCTA TAAGGTGAAA ATAAAGGAAC TTGAAGCAAT GTTAGAAACT
    CAGAAAGATG AATGTAGCCA TTCAGCCAAG TTAGAACAAG AAATTATGGA AAAGGAATCT
    ATCATTTTAA AGCAAGAAAG AAATCTGAAT GAATGTCAAG CAAATCTTAA AGATTCTATC
    CAAAATGCCA GAGATTTAAG TGAAAGGGAG GTCAAATTGA AAGAAGAAAT CATGCAATTA
    ACAAAAAATT TGCAAGATGC TAAGCAATCA CTTCAATTAA AGGAAGAAGA AAAGGAAAGA
    AACTGGCAAG AAACAGAAAA GTTGAAAGAG GAGCTCTCTG CAAGCACTAC TCTTACCCAG
    AATCTGAAAG CTGATCTTCA GAGGAAGGAA GAAGATTATG CTGAGCTCAA AGAGAAACTG
    GCTGATGCCA AAAAGCAGAT TGAGCAAGTA CAGAAAGAGA TCTCTGTAAT GCGTGATGAG
    GAGAAATTGT TGAGGATTAA AATTAATGAA CTGGAGAAAA AGAAAAACCA GTGTTCTCAG
    GAAATAGATA TGAAACAGCG AACCATTCAG CAACTTAAGG AGCAGTTAAA TAATCAAAAA
    GTGGAAGAAG CTATACAACA GTATGAGAGA GTGTGCAAAG ATCTGAGTGT TAAAGAAAAA
    ATAATCAAAG ACATGCAAAT GACCCTGGAA GAACAAGAGC AGACTCAGGT AGAACAAGAT
    CAAGTGCTTG AGGCTAAATT AGAGGAAACC GAAAGACTGG CCACAGAATT GGAAGAATGG
    AAGGAAAAAT ACAAAGATTT GGAAGCCAAA CACAATCAAA GTTCAAATAA AGAATTTGAG
    GATAACACAG ATGTACTTAA TACAAAACTC AGTAAGCTTC AAGATGAGTT ACAGGAATCG
    GAACAGAAAC ATGAAGCTGA AAGAAAAAAA TGGTTAGAAG AAAAAATGAT GCTTATCACT
    CAAGCAAAAG AAGCTGAGAA TCTACGAAAC AAAGAGATGA AAAAATATGC CGAAGACAGA
    GAGCATTGTT TGAAGCAACA AAATGAAATG GAAATACTTA AAGGACAGTT GGCTGAGAAA
    GATGGTAACC TCCAGCAGTG GCGGGAAGAA CGCGATCAAC TTGTTGCAGC TTTAGAAATA
    CAGCTGAAAG CACTGATCTC CAGTAATGTA CAAAAAGATA ATGAAATCGA ACAATTAAAA
    AAGATCACAT CAGAGGCTTC TAAGACAGAA AAACAGACCA TGGATATCCA CCTCACACAA
    ATGAGTTTAA CAGATCCTGG CAGGGTTGAA ATTGAACCTC AGTCAACAAG TTTTGAAATT
    TCCAGAGGTG AAGTAGAGGA TGGATCTGTA GTCCTTGACT CTTGTGAAGT GTCGACAGAA
    AATAATCAAA GCACTCGATT TCCAAAACCT GAGTTAGAGA TTCAATTTAC ACCTTTACAG
    CCAAACAGAA TGGCAGTGAA ACACCCAGGT TGTACCTCAC CAGTGACGGT TAAGATTCCC
    AAGGCCCGAA AGAGGAAAAG TCATGAAATG GAAGAGGACT TTGTGAAATT TGAAAATAAG
    AAGAATGCTA CACCAAGAAT TAATTTGAAG TCCCCTGTTT CTGAGCATAG AAATTCTTTC
    AAAAAGGAAC AAAAGGTTTC CATACGCCCA TCATCTAAGA AAACTTATTC TTTACGGAGT
    CATGCATCCA CAGTTGGTGT AAACGGGTCT GCTAAGAAAA AAGAAGGAAC GCTACAGAAA
    TTTGGAGACT TCTTACAACA TTCTCCAACT ATTCTTCAGT CAAAAGCAAA GAAGATAATT
    GAAACAATGA GCTCTTCAAA GTTCTCAAAT GTAGAAGTAA GTAAAGAAAA TGTGTCTCGA
    CCAAAACGGG CCAAACGAAA ATTATACACA AATGAAATTT CATCTCCTAT TGATATATCT
    GGCCAAGTGA TTTTGATGGA CCGTAAAGTG AAAGAAAGCG ATCATCAGAT TCTCAAACGA
    CGACTTCGAA CCAGAACAGT CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33439
    Clone ID Related Accession (Same CDS sequence) XM_005225362.3
    Accession Version XM_005225362.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF20B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104580.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225362.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGGGACT CCGTACATAC CAGAATGGAA TCTAATTTAA ATCAAGATGG GATGTCTAGA 
    CCATCTTATG TTTTCAGTGC TGACCCACTT GCAAGACCTT CAGAAATAAA TTTTGATGGC
    ATTAAGCTTG ACCTCTCTCG TGAATTTTCC TTAGTTGCTT CAAGCACTGA GGCAAACAGT
    TCTGAATCTA AAGATTATCT CCAAGTTTGT CTTCGAGTAA GACCATTCAC ACAGCCAGAA
    AAAGAACATG AGTCTGAGGG CTGTGTGTAT ATTCTGGATT CACAGACTGT TGTCCTGAAG
    GATCCTCAGT ATACTACCCT TGGTCGTTTA AGTGAGAAAA GCTCTGGGCA GATGGCACAA
    AAATTCAGTT TTTCCAAGGT TTTTGGCCCA GAAACTACAC AGAAGGAATT CTTCCAGGGT
    TGCATTCTGC AGCCAGTTAA AGACCTCTTG AAAGGACAGA GTCGGCTGAT TTTTACTTAC
    GGGCTAACCA ATTCTGGAAA AACTTACACG TTTCAAGGCA CAGAAGAAAA TACTGGCATT
    CTTCCTCGAA CTTTGAATGT ATTATTTGAT AGTCTTCAGG AAAGACTGTA TACAAAGATG
    AACCTTAAAC CACATAGGTC CAGAGAATAC TTACGGTTAT CACCAGACCA AGAGAAAGAA
    GAAGTCGCTA GCAAAAGTGC ATTACTTCGG CAAATTAAAG AGGTTGTTAT GCATAATGAC
    AGTTATGGTG CTCTTTATGG AAGTCTAACA AACTCTTTGA GTATCCCAGA GTTTGAAGAA
    TCCATGAAAG ATTGTGAACA CTCAAACTTG AACATAGATA TTAATAGAAA ATTTTCTGTG
    TGGGTATCTT TCTTTGAGAT TTACAACGAA TGTATTTACG ATTTGTTTGT TCCTGTATCA
    TCTAAGTTCC AAAAGAGAAA GACACTGCGC CTTTCCCAAG ATGTAAAGGG CTATTCTTTC
    ATAAAAGATC TACAGTGGAT TCAAGTATCT GATGCCAAAG AAGCATATAG ACTTTTAAAG
    CTAGGAACAA AGCACCAGAG TGTTGCTTTC ACAAAACTGA ACAATGCTTC TAGTAGAAGT
    CACAGCATAT TTACCATTAG AATATTACAG ATTGAAGATT CAGAAATACC ACATGTAATA
    CGAGTCAGTG AATTGTCTTT ATGTGATCTT GCTGGCTCAG AACGAAGCAT GAGGACACAG
    AATGAAGGTG AAAGGTTAAG AGAGACCGGG AATATCAACA CTTCTTTATT GACTCTGGGA
    AAGTGTATCA GTGTTTTGAA GAATAGCGAA AAGTCAAAGT TTCAACAGCA TGTGCCTTTC
    CGGGAAAGTA AATTGACCCA CTATTTTCAA AGTTTTTTTA ATGGTAAAGG GAAGATATGT
    ATGATTGTCA ACATTAGCCA ATGTTCTTTT ACCTATGATG AGACACTCAA TGTGTTGAAG
    TTCTCAGCCA TTGCACAAAA AGTTTATGTT CCAGATATTT TAAATTGCTC TCAAGAGAAA
    TCATTTGGAC CTGTCAAATC TTCTCAAGAT GAATCATTAG ATATTAATAA TTCAGATAAT
    AAAATACTAA ATGAAAAAAG ATCCACAATT TCATGGGAAA GTAGCCTGGA AGATGTGGTG
    GAAGATGAAG ATTTGGTTGA GGATCTAGAA AAAGCTGAAG AAAAGCAGAA TGTGGAAACT
    GAATTTATTG ATGAAGATCT ACATGATACA TTAGAGGATG ATAAGGCTTT TAGTAGCCAT
    GCAGGGAAGA GAAAACTTCT GGATTTAATA GAAGATTTGA AAAAAAAACT AATAAATGAA
    AGAAAGGAAA AGTTAACATT GGAATTTAAA ATTCGTGACG AAGTTACACA GGAGTTTACT
    CAGTATTTGG CCCAACGAGA AGCTGACTTT AAGGAAACTC TCCTTCAGGA ACGAGAAATT
    TTAGAAGAAA ATGCTGAATG TCGTTTGGCT ATTTTCAAGG ATTTGATTGG TAAATGTGAT
    GCTCAGGAGG AACCAAAGAA TGAAGATCAT GCCATAAAAG ATGAAACTGA AGAAGGACAT
    AATTATGTAG GAATTGAAGA TATTATTGAT TCTCTTCAAG ATGATGTCAC TGGTATTAAG
    AAACAGGCTG AAATTGCTCA CTTATATATT GCATCTCTTG CTGACCCCCA GGAAGCTATT
    GCTTGTTTAG AAATAAAGTT TAATCAAGTT AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA
    TTAATCAAAA CCCGAGAAGA GTTAAAAAAG AGAGAAAGTG AATCAGAAAT TAATTTAAAT
    TCATTGGTTC AAGAGCTTGA GAAATCTAAT AAGAAAATAA TTATGCAGAA TCAAAGAATT
    CAAGAGTTGA TGGATAAAAT TGACCAAAAA GAGGATACTA TTAACAAATT TCAGAACCTA
    AAATTTCATA TGGAAAACAC ATGTAAAGGC AGTGACAATG TTGGTAATTC TTCTTTAATA
    ATAAACAGTA AATTGGTTTG TGATGAAACG ACTGAAATGT CTAAGGACAG CAAAACTAAA
    ACGTATTCAG GAAGAAAAAG ATTAAATGAA AACGAACTTC AACAAGATGA ACCACCAGCA
    AAGAAAGGAC TTACGCATGC TAGTCCAGCT ATCACTGAAG ACCAAAAGAA AAGTGAAGAA
    ATGCAAGAGA GCATTTCAGA TGAGGAAAAC ATTAGACTTC TACAAGAAAA TAATGAAGAG
    CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA
    GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT
    TTAGGCTTTA GTATAGAGAT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA
    TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATGAA ATTGTCAGAG
    GAGATAGAAA CTGCTAGACG AAACATCACA AACAATGTTT CACAAATAAA ATTAATGCAA
    GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTTTCTC AGATCGCAAA CATAGATTTA
    CTCAATCTCA GGGATCTGTC CAGTTGTTCT CAAGAAGATA ATTTGCCAAA CACACAGTTA
    CATCATTTGG ATAATGATTG TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA
    CTTAGTAGGG AAAGGTCTTT CCACTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG
    ATTGTAAAGA CTTCCTCCAA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA
    AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ATAGACTAAA AATAGAGGAG
    AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG
    AAGGAAGAAG TGCAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA
    GGAAAAAGAG CACTTTCAGA ACTTATGCAA GATGTTACTT GCTATAAGGT GAAAATAAAG
    GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA
    CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT
    CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAGGTCAAA
    TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA ATCACTTCAA
    TTAAAGGAAG AAGAAAAGGA AAGAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC
    TCTGCAAGCA CTACTCTTAC CCAGAATCTG AAAGCTGATC TTCAGAGGAA GGAAGAAGAT
    TATGCTGAGC TCAAAGAGAA ACTGGCTGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA
    GAGATCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG
    AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT
    AAGGAGCAGT TAAATAATCA AAAAGTGGAA GAAGCTATAC AACAGTATGA GAGAGTGTGC
    AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA
    GAGCAGACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA
    CTGGCCACAG AATTGGAAGA ATGGAAGGAA AAATACAAAG ATTTGGAAGC CAAACACAAT
    CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCAGTAAG
    CTTCAAGATG AGTTACAGGA ATCGGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA
    GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAATCTACG AAACAAAGAG
    ATGAAAAAAT ATGCCGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGA AATGGAAATA
    CTTAAAGGAC AGTTGGCTGA GAAAGATGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT
    CAACTTGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA
    GATAATGAAA TCGAACAATT AAAAAAGATC ACATCAGAGG CTTCTAAGAC AGAAAAACAG
    ACCATGGATA TCCACCTCAC ACAAATGAGT TTAACAGATC CTGGCAGGGT TGAAATTGAA
    CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT
    GACTCTTGTG AAGTGTCGAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA
    GAGATTCAAT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC
    TCACCAGTGA CGGTTAAGAT TCCCAAGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG
    GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT
    GTTTCTGAGC ATAGAAATTC TTTCAAAAAG GAACAAAAGG TTTCCATACG CCCATCATCT
    AAGAAAACTT ATTCTTTACG GAGTCATGCA TCCACAGTTG GTGTAAACGG GTCTGCTAAG
    AAAAAAGAAG GAACGCTACA GAAATTTGGA GACTTCTTAC AACATTCTCC AACTATTCTT
    CAGTCAAAAG CAAAGAAGAT AATTGAAACA ATGAGCTCTT CAAAGTTCTC AAATGTAGAA
    GTAAGTAAAG AAAATGTGTC TCGACCAAAA CGGGCCAAAC GAAAATTATA CACAAATGAA
    ATTTCATCTC CTATTGATAT ATCTGGCCAA GTGATTTTGA TGGACCGTAA AGTGAAAGAA
    AGCGATCATC AGATTCTCAA ACGACGACTT CGAACCAGAA CAGTCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225419.1
    CDS131..5641
    Translation

    Target ORF information:

    RefSeq Version XM_005225362.3
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 20B (KIF20B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225362.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGGGACT CCGTACATAC CAGAATGGAA TCTAATTTAA ATCAAGATGG GATGTCTAGA 
    CCATCTTATG TTTTCAGTGC TGACCCACTT GCAAGACCTT CAGAAATAAA TTTTGATGGC
    ATTAAGCTTG ACCTCTCTCG TGAATTTTCC TTAGTTGCTT CAAGCACTGA GGCAAACAGT
    TCTGAATCTA AAGATTATCT CCAAGTTTGT CTTCGAGTAA GACCATTCAC ACAGCCAGAA
    AAAGAACATG AGTCTGAGGG CTGTGTGTAT ATTCTGGATT CACAGACTGT TGTCCTGAAG
    GATCCTCAGT ATACTACCCT TGGTCGTTTA AGTGAGAAAA GCTCTGGGCA GATGGCACAA
    AAATTCAGTT TTTCCAAGGT TTTTGGCCCA GAAACTACAC AGAAGGAATT CTTCCAGGGT
    TGCATTCTGC AGCCAGTTAA AGACCTCTTG AAAGGACAGA GTCGGCTGAT TTTTACTTAC
    GGGCTAACCA ATTCTGGAAA AACTTACACG TTTCAAGGCA CAGAAGAAAA TACTGGCATT
    CTTCCTCGAA CTTTGAATGT ATTATTTGAT AGTCTTCAGG AAAGACTGTA TACAAAGATG
    AACCTTAAAC CACATAGGTC CAGAGAATAC TTACGGTTAT CACCAGACCA AGAGAAAGAA
    GAAGTCGCTA GCAAAAGTGC ATTACTTCGG CAAATTAAAG AGGTTGTTAT GCATAATGAC
    AGTTATGGTG CTCTTTATGG AAGTCTAACA AACTCTTTGA GTATCCCAGA GTTTGAAGAA
    TCCATGAAAG ATTGTGAACA CTCAAACTTG AACATAGATA TTAATAGAAA ATTTTCTGTG
    TGGGTATCTT TCTTTGAGAT TTACAACGAA TGTATTTACG ATTTGTTTGT TCCTGTATCA
    TCTAAGTTCC AAAAGAGAAA GACACTGCGC CTTTCCCAAG ATGTAAAGGG CTATTCTTTC
    ATAAAAGATC TACAGTGGAT TCAAGTATCT GATGCCAAAG AAGCATATAG ACTTTTAAAG
    CTAGGAACAA AGCACCAGAG TGTTGCTTTC ACAAAACTGA ACAATGCTTC TAGTAGAAGT
    CACAGCATAT TTACCATTAG AATATTACAG ATTGAAGATT CAGAAATACC ACATGTAATA
    CGAGTCAGTG AATTGTCTTT ATGTGATCTT GCTGGCTCAG AACGAAGCAT GAGGACACAG
    AATGAAGGTG AAAGGTTAAG AGAGACCGGG AATATCAACA CTTCTTTATT GACTCTGGGA
    AAGTGTATCA GTGTTTTGAA GAATAGCGAA AAGTCAAAGT TTCAACAGCA TGTGCCTTTC
    CGGGAAAGTA AATTGACCCA CTATTTTCAA AGTTTTTTTA ATGGTAAAGG GAAGATATGT
    ATGATTGTCA ACATTAGCCA ATGTTCTTTT ACCTATGATG AGACACTCAA TGTGTTGAAG
    TTCTCAGCCA TTGCACAAAA AGTTTATGTT CCAGATATTT TAAATTGCTC TCAAGAGAAA
    TCATTTGGAC CTGTCAAATC TTCTCAAGAT GAATCATTAG ATATTAATAA TTCAGATAAT
    AAAATACTAA ATGAAAAAAG ATCCACAATT TCATGGGAAA GTAGCCTGGA AGATGTGGTG
    GAAGATGAAG ATTTGGTTGA GGATCTAGAA AAAGCTGAAG AAAAGCAGAA TGTGGAAACT
    GAATTTATTG ATGAAGATCT ACATGATACA TTAGAGGATG ATAAGGCTTT TAGTAGCCAT
    GCAGGGAAGA GAAAACTTCT GGATTTAATA GAAGATTTGA AAAAAAAACT AATAAATGAA
    AGAAAGGAAA AGTTAACATT GGAATTTAAA ATTCGTGACG AAGTTACACA GGAGTTTACT
    CAGTATTTGG CCCAACGAGA AGCTGACTTT AAGGAAACTC TCCTTCAGGA ACGAGAAATT
    TTAGAAGAAA ATGCTGAATG TCGTTTGGCT ATTTTCAAGG ATTTGATTGG TAAATGTGAT
    GCTCAGGAGG AACCAAAGAA TGAAGATCAT GCCATAAAAG ATGAAACTGA AGAAGGACAT
    AATTATGTAG GAATTGAAGA TATTATTGAT TCTCTTCAAG ATGATGTCAC TGGTATTAAG
    AAACAGGCTG AAATTGCTCA CTTATATATT GCATCTCTTG CTGACCCCCA GGAAGCTATT
    GCTTGTTTAG AAATAAAGTT TAATCAAGTT AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA
    TTAATCAAAA CCCGAGAAGA GTTAAAAAAG AGAGAAAGTG AATCAGAAAT TAATTTAAAT
    TCATTGGTTC AAGAGCTTGA GAAATCTAAT AAGAAAATAA TTATGCAGAA TCAAAGAATT
    CAAGAGTTGA TGGATAAAAT TGACCAAAAA GAGGATACTA TTAACAAATT TCAGAACCTA
    AAATTTCATA TGGAAAACAC ATGTAAAGGC AGTGACAATG TTGGTAATTC TTCTTTAATA
    ATAAACAGTA AATTGGTTTG TGATGAAACG ACTGAAATGT CTAAGGACAG CAAAACTAAA
    ACGTATTCAG GAAGAAAAAG ATTAAATGAA AACGAACTTC AACAAGATGA ACCACCAGCA
    AAGAAAGGAC TTACGCATGC TAGTCCAGCT ATCACTGAAG ACCAAAAGAA AAGTGAAGAA
    ATGCAAGAGA GCATTTCAGA TGAGGAAAAC ATTAGACTTC TACAAGAAAA TAATGAAGAG
    CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA
    GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT
    TTAGGCTTTA GTATAGAGAT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA
    TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATGAA ATTGTCAGAG
    GAGATAGAAA CTGCTAGACG AAACATCACA AACAATGTTT CACAAATAAA ATTAATGCAA
    GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTTTCTC AGATCGCAAA CATAGATTTA
    CTCAATCTCA GGGATCTGTC CAGTTGTTCT CAAGAAGATA ATTTGCCAAA CACACAGTTA
    CATCATTTGG ATAATGATTG TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA
    CTTAGTAGGG AAAGGTCTTT CCACTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG
    ATTGTAAAGA CTTCCTCCAA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA
    AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ATAGACTAAA AATAGAGGAG
    AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG
    AAGGAAGAAG TGCAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA
    GGAAAAAGAG CACTTTCAGA ACTTATGCAA GATGTTACTT GCTATAAGGT GAAAATAAAG
    GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA
    CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT
    CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAGGTCAAA
    TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA ATCACTTCAA
    TTAAAGGAAG AAGAAAAGGA AAGAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC
    TCTGCAAGCA CTACTCTTAC CCAGAATCTG AAAGCTGATC TTCAGAGGAA GGAAGAAGAT
    TATGCTGAGC TCAAAGAGAA ACTGGCTGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA
    GAGATCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG
    AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT
    AAGGAGCAGT TAAATAATCA AAAAGTGGAA GAAGCTATAC AACAGTATGA GAGAGTGTGC
    AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA
    GAGCAGACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA
    CTGGCCACAG AATTGGAAGA ATGGAAGGAA AAATACAAAG ATTTGGAAGC CAAACACAAT
    CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCAGTAAG
    CTTCAAGATG AGTTACAGGA ATCGGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA
    GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAATCTACG AAACAAAGAG
    ATGAAAAAAT ATGCCGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGA AATGGAAATA
    CTTAAAGGAC AGTTGGCTGA GAAAGATGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT
    CAACTTGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA
    GATAATGAAA TCGAACAATT AAAAAAGATC ACATCAGAGG CTTCTAAGAC AGAAAAACAG
    ACCATGGATA TCCACCTCAC ACAAATGAGT TTAACAGATC CTGGCAGGGT TGAAATTGAA
    CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT
    GACTCTTGTG AAGTGTCGAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA
    GAGATTCAAT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC
    TCACCAGTGA CGGTTAAGAT TCCCAAGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG
    GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT
    GTTTCTGAGC ATAGAAATTC TTTCAAAAAG GAACAAAAGG TTTCCATACG CCCATCATCT
    AAGAAAACTT ATTCTTTACG GAGTCATGCA TCCACAGTTG GTGTAAACGG GTCTGCTAAG
    AAAAAAGAAG GAACGCTACA GAAATTTGGA GACTTCTTAC AACATTCTCC AACTATTCTT
    CAGTCAAAAG CAAAGAAGAT AATTGAAACA ATGAGCTCTT CAAAGTTCTC AAATGTAGAA
    GTAAGTAAAG AAAATGTGTC TCGACCAAAA CGGGCCAAAC GAAAATTATA CACAAATGAA
    ATTTCATCTC CTATTGATAT ATCTGGCCAA GTGATTTTGA TGGACCGTAA AGTGAAAGAA
    AGCGATCATC AGATTCTCAA ACGACGACTT CGAACCAGAA CAGTCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33440
    Clone ID Related Accession (Same CDS sequence) XM_005225363.3
    Accession Version XM_005225363.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF20B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104580.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225363.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGGGACT CCGTACATAC CAGAATGGAA TCTAATTTAA ATCAAGATGG GATGTCTAGA 
    CCATCTTATG TTTTCAGTGC TGACCCACTT GCAAGACCTT CAGAAATAAA TTTTGATGGC
    ATTAAGCTTG ACCTCTCTCG TGAATTTTCC TTAGTTGCTT CAAGCACTGA GGCAAACAGT
    TCTGAATCTA AAGATTATCT CCAAGTTTGT CTTCGAGTAA GACCATTCAC ACAGCCAGAA
    AAAGAACATG AGTCTGAGGG CTGTGTGTAT ATTCTGGATT CACAGACTGT TGTCCTGAAG
    GATCCTCAGT ATACTACCCT TGGTCGTTTA AGTGAGAAAA GCTCTGGGCA GATGGCACAA
    AAATTCAGTT TTTCCAAGGT TTTTGGCCCA GAAACTACAC AGAAGGAATT CTTCCAGGGT
    TGCATTCTGC AGCCAGTTAA AGACCTCTTG AAAGGACAGA GTCGGCTGAT TTTTACTTAC
    GGGCTAACCA ATTCTGGAAA AACTTACACG TTTCAAGGCA CAGAAGAAAA TACTGGCATT
    CTTCCTCGAA CTTTGAATGT ATTATTTGAT AGTCTTCAGG AAAGACTGTA TACAAAGATG
    AACCTTAAAC CACATAGGTC CAGAGAATAC TTACGGTTAT CACCAGACCA AGAGAAAGAA
    GAAGTCGCTA GCAAAAGTGC ATTACTTCGG CAAATTAAAG AGGTTGTTAT GCATAATGAC
    AGTTATGGTG CTCTTTATGG AAGTCTAACA AACTCTTTGA GTATCCCAGA GTTTGAAGAA
    TCCATGAAAG ATTGTGAACA CTCAAACTTG AACATAGATA TTAATAGAAA ATTTTCTGTG
    TGGGTATCTT TCTTTGAGAT TTACAACGAA TGTATTTACG ATTTGTTTGT TCCTGTATCA
    TCTAAGTTCC AAAAGAGAAA GACACTGCGC CTTTCCCAAG ATGTAAAGGG CTATTCTTTC
    ATAAAAGATC TACAGTGGAT TCAAGTATCT GATGCCAAAG AAGCATATAG ACTTTTAAAG
    CTAGGAACAA AGCACCAGAG TGTTGCTTTC ACAAAACTGA ACAATGCTTC TAGTAGAAGT
    CACAGCATAT TTACCATTAG AATATTACAG ATTGAAGATT CAGAAATACC ACATGTAATA
    CGAGTCAGTG AATTGTCTTT ATGTGATCTT GCTGGCTCAG AACGAAGCAT GAGGACACAG
    AATGAAGGTG AAAGGTTAAG AGAGACCGGG AATATCAACA CTTCTTTATT GACTCTGGGA
    AAGTGTATCA GTGTTTTGAA GAATAGCGAA AAGTCAAAGT TTCAACAGCA TGTGCCTTTC
    CGGGAAAGTA AATTGACCCA CTATTTTCAA AGTTTTTTTA ATGGTAAAGG GAAGATATGT
    ATGATTGTCA ACATTAGCCA ATGTTCTTTT ACCTATGATG AGACACTCAA TGTGTTGAAG
    TTCTCAGCCA TTGCACAAAA AGTTTATGTT CCAGATATTT TAAATTGCTC TCAAGAGAAA
    TCATTTGGAC CTGTCAAATC TTCTCAAGAT GAATCATTAG ATATTAATAA TTCAGATAAT
    AAAATACTAA ATGAAAAAAG ATCCACAATT TCATGGGAAA GTAGCCTGGA AGATGTGGTG
    GAAGATGAAG ATTTGGTTGA GGATCTAGAA AAAGCTGAAG AAAAGCAGAA TGTGGAAACT
    GAATTTATTG ATGAAGATCT ACATGATACA TTAGAGGATG ATAAGGCTTT TAGTAGCCAT
    GCAGGGAAGA GAAAACTTCT GGATTTAATA GAAGATTTGA AAAAAAAACT AATAAATGAA
    AGAAAGGAAA AGTTAACATT GGAATTTAAA ATTCGTGACG AAGTTACACA GGAGTTTACT
    CAGTATTTGG CCCAACGAGA AGCTGACTTT AAGGAAACTC TCCTTCAGGA ACGAGAAATT
    TTAGAAGAAA ATGCTGAATG TCGTTTGGCT ATTTTCAAGG ATTTGATTGG TAAATGTGAT
    GCTCAGGAGG AACCAAAGAA TGAAGATCAT GCCATAAAAG ATGAAACTGA AGAAGCTATT
    GCTTGTTTAG AAATAAAGTT TAATCAAGTT AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA
    TTAATCAAAA CCCGAGAAGA GTTAAAAAAG AGAGAAAGTG AATCAGAAAT TAATTTAAAT
    TCATTGGTTC AAGAGCTTGA GAAATCTAAT AAGAAAATAA TTATGCAGAA TCAAAGAATT
    CAAGAGTTGA TGGATAAAAT TGACCAAAAA GAGGATACTA TTAACAAATT TCAGAACCTA
    AAATTTCATA TGGAAAACAC ATGTAAAGGC AGTGACAATG TTGGTAATTC TTCTTTAATA
    ATAAACAGTA AATTGGTTTG TGATGAAACG ACTGAAATGT CTAAGGACAG CAAAACTAAA
    ACGTATTCAG GAAGAAAAAG ATTAAATGAA AACGAACTTC AACAAGATGA ACCACCAGCA
    AAGAAAGGAC TTACGCATGC TAGTCCAGCT ATCACTGAAG ACCAAAAGAA AAGTGAAGAA
    ATGCAAGAGA GCATTTCAGA TGAGGAAAAC ATTAGACTTC TACAAGAAAA TAATGAAGAG
    CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA
    GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT
    TTAGGCTTTA GTATAGAGAT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA
    TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATGAA ATTGTCAGAG
    GAGATAGAAA CTGCTAGACG AAACATCACA AACAATGTTT CACAAATAAA ATTAATGCAA
    GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTTTCTC AGATCGCAAA CATAGATTTA
    CTCAATCTCA GGGATCTGTC CAGTTGTTCT CAAGAAGATA ATTTGCCAAA CACACAGTTA
    CATCATTTGG ATAATGATTG TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA
    CTTAGTAGGG AAAGGTCTTT CCACTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG
    ATTGTAAAGA CTTCCTCCAA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA
    AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ATAGACTAAA AATAGAGGAG
    AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG
    AAGGAAGAAG TGCAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA
    GGAAAAAGAG CACTTTCAGA ACTTATGCAA GATGTTACTT GCTATAAGGT GAAAATAAAG
    GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA
    CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT
    CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAGGTCAAA
    TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA ATCACTTCAA
    TTAAAGGAAG AAGAAAAGGA AAGAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC
    TCTGCAAGCA CTACTCTTAC CCAGAATCTG AAAGCTGATC TTCAGAGGAA GGAAGAAGAT
    TATGCTGAGC TCAAAGAGAA ACTGGCTGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA
    GAGATCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG
    AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT
    AAGGAGCAGT TAAATAATCA AAAAGTGGAA GAAGCTATAC AACAGTATGA GAGAGTGTGC
    AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA
    GAGCAGACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA
    CTGGCCACAG AATTGGAAGA ATGGAAGGAA AAATACAAAG ATTTGGAAGC CAAACACAAT
    CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCAGTAAG
    CTTCAAGATG AGTTACAGGA ATCGGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA
    GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAATCTACG AAACAAAGAG
    ATGAAAAAAT ATGCCGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGA AATGGAAATA
    CTTAAAGGAC AGTTGGCTGA GAAAGATGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT
    CAACTTGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA
    GATAATGAAA TCGAACAATT AAAAAAGATC ACATCAGAGG CTTCTAAGAC AGAAAAACAG
    ACCATGGATA TCCACCTCAC ACAAATGAGT TTAACAGATC CTGGCAGGGT TGAAATTGAA
    CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT
    GACTCTTGTG AAGTGTCGAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA
    GAGATTCAAT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC
    TCACCAGTGA CGGTTAAGAT TCCCAAGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG
    GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT
    GTTTCTGAGC ATAGAAATTC TTTCAAAAAG GAACAAAAGG TTTCCATACG CCCATCATCT
    AAGAAAACTT ATTCTTTACG GAGTCATGCA TCCACAGTTG GTGTAAACGG GTCTGCTAAG
    AAAAAAGAAG GAACGCTACA GAAATTTGGA GACTTCTTAC AACATTCTCC AACTATTCTT
    CAGTCAAAAG CAAAGAAGAT AATTGAAACA ATGAGCTCTT CAAAGTTCTC AAATGTAGAA
    GTAAGTAAAG AAAATGTGTC TCGACCAAAA CGGGCCAAAC GAAAATTATA CACAAATGAA
    ATTTCATCTC CTATTGATAT ATCTGGCCAA GTGATTTTGA TGGACCGTAA AGTGAAAGAA
    AGCGATCATC AGATTCTCAA ACGACGACTT CGAACCAGAA CAGTCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225420.1
    CDS131..5521
    Translation

    Target ORF information:

    RefSeq Version XM_005225363.3
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 20B (KIF20B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225363.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGGGACT CCGTACATAC CAGAATGGAA TCTAATTTAA ATCAAGATGG GATGTCTAGA 
    CCATCTTATG TTTTCAGTGC TGACCCACTT GCAAGACCTT CAGAAATAAA TTTTGATGGC
    ATTAAGCTTG ACCTCTCTCG TGAATTTTCC TTAGTTGCTT CAAGCACTGA GGCAAACAGT
    TCTGAATCTA AAGATTATCT CCAAGTTTGT CTTCGAGTAA GACCATTCAC ACAGCCAGAA
    AAAGAACATG AGTCTGAGGG CTGTGTGTAT ATTCTGGATT CACAGACTGT TGTCCTGAAG
    GATCCTCAGT ATACTACCCT TGGTCGTTTA AGTGAGAAAA GCTCTGGGCA GATGGCACAA
    AAATTCAGTT TTTCCAAGGT TTTTGGCCCA GAAACTACAC AGAAGGAATT CTTCCAGGGT
    TGCATTCTGC AGCCAGTTAA AGACCTCTTG AAAGGACAGA GTCGGCTGAT TTTTACTTAC
    GGGCTAACCA ATTCTGGAAA AACTTACACG TTTCAAGGCA CAGAAGAAAA TACTGGCATT
    CTTCCTCGAA CTTTGAATGT ATTATTTGAT AGTCTTCAGG AAAGACTGTA TACAAAGATG
    AACCTTAAAC CACATAGGTC CAGAGAATAC TTACGGTTAT CACCAGACCA AGAGAAAGAA
    GAAGTCGCTA GCAAAAGTGC ATTACTTCGG CAAATTAAAG AGGTTGTTAT GCATAATGAC
    AGTTATGGTG CTCTTTATGG AAGTCTAACA AACTCTTTGA GTATCCCAGA GTTTGAAGAA
    TCCATGAAAG ATTGTGAACA CTCAAACTTG AACATAGATA TTAATAGAAA ATTTTCTGTG
    TGGGTATCTT TCTTTGAGAT TTACAACGAA TGTATTTACG ATTTGTTTGT TCCTGTATCA
    TCTAAGTTCC AAAAGAGAAA GACACTGCGC CTTTCCCAAG ATGTAAAGGG CTATTCTTTC
    ATAAAAGATC TACAGTGGAT TCAAGTATCT GATGCCAAAG AAGCATATAG ACTTTTAAAG
    CTAGGAACAA AGCACCAGAG TGTTGCTTTC ACAAAACTGA ACAATGCTTC TAGTAGAAGT
    CACAGCATAT TTACCATTAG AATATTACAG ATTGAAGATT CAGAAATACC ACATGTAATA
    CGAGTCAGTG AATTGTCTTT ATGTGATCTT GCTGGCTCAG AACGAAGCAT GAGGACACAG
    AATGAAGGTG AAAGGTTAAG AGAGACCGGG AATATCAACA CTTCTTTATT GACTCTGGGA
    AAGTGTATCA GTGTTTTGAA GAATAGCGAA AAGTCAAAGT TTCAACAGCA TGTGCCTTTC
    CGGGAAAGTA AATTGACCCA CTATTTTCAA AGTTTTTTTA ATGGTAAAGG GAAGATATGT
    ATGATTGTCA ACATTAGCCA ATGTTCTTTT ACCTATGATG AGACACTCAA TGTGTTGAAG
    TTCTCAGCCA TTGCACAAAA AGTTTATGTT CCAGATATTT TAAATTGCTC TCAAGAGAAA
    TCATTTGGAC CTGTCAAATC TTCTCAAGAT GAATCATTAG ATATTAATAA TTCAGATAAT
    AAAATACTAA ATGAAAAAAG ATCCACAATT TCATGGGAAA GTAGCCTGGA AGATGTGGTG
    GAAGATGAAG ATTTGGTTGA GGATCTAGAA AAAGCTGAAG AAAAGCAGAA TGTGGAAACT
    GAATTTATTG ATGAAGATCT ACATGATACA TTAGAGGATG ATAAGGCTTT TAGTAGCCAT
    GCAGGGAAGA GAAAACTTCT GGATTTAATA GAAGATTTGA AAAAAAAACT AATAAATGAA
    AGAAAGGAAA AGTTAACATT GGAATTTAAA ATTCGTGACG AAGTTACACA GGAGTTTACT
    CAGTATTTGG CCCAACGAGA AGCTGACTTT AAGGAAACTC TCCTTCAGGA ACGAGAAATT
    TTAGAAGAAA ATGCTGAATG TCGTTTGGCT ATTTTCAAGG ATTTGATTGG TAAATGTGAT
    GCTCAGGAGG AACCAAAGAA TGAAGATCAT GCCATAAAAG ATGAAACTGA AGAAGCTATT
    GCTTGTTTAG AAATAAAGTT TAATCAAGTT AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA
    TTAATCAAAA CCCGAGAAGA GTTAAAAAAG AGAGAAAGTG AATCAGAAAT TAATTTAAAT
    TCATTGGTTC AAGAGCTTGA GAAATCTAAT AAGAAAATAA TTATGCAGAA TCAAAGAATT
    CAAGAGTTGA TGGATAAAAT TGACCAAAAA GAGGATACTA TTAACAAATT TCAGAACCTA
    AAATTTCATA TGGAAAACAC ATGTAAAGGC AGTGACAATG TTGGTAATTC TTCTTTAATA
    ATAAACAGTA AATTGGTTTG TGATGAAACG ACTGAAATGT CTAAGGACAG CAAAACTAAA
    ACGTATTCAG GAAGAAAAAG ATTAAATGAA AACGAACTTC AACAAGATGA ACCACCAGCA
    AAGAAAGGAC TTACGCATGC TAGTCCAGCT ATCACTGAAG ACCAAAAGAA AAGTGAAGAA
    ATGCAAGAGA GCATTTCAGA TGAGGAAAAC ATTAGACTTC TACAAGAAAA TAATGAAGAG
    CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA
    GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT
    TTAGGCTTTA GTATAGAGAT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA
    TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATGAA ATTGTCAGAG
    GAGATAGAAA CTGCTAGACG AAACATCACA AACAATGTTT CACAAATAAA ATTAATGCAA
    GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTTTCTC AGATCGCAAA CATAGATTTA
    CTCAATCTCA GGGATCTGTC CAGTTGTTCT CAAGAAGATA ATTTGCCAAA CACACAGTTA
    CATCATTTGG ATAATGATTG TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA
    CTTAGTAGGG AAAGGTCTTT CCACTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG
    ATTGTAAAGA CTTCCTCCAA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA
    AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ATAGACTAAA AATAGAGGAG
    AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG
    AAGGAAGAAG TGCAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA
    GGAAAAAGAG CACTTTCAGA ACTTATGCAA GATGTTACTT GCTATAAGGT GAAAATAAAG
    GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA
    CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT
    CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAGGTCAAA
    TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA ATCACTTCAA
    TTAAAGGAAG AAGAAAAGGA AAGAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC
    TCTGCAAGCA CTACTCTTAC CCAGAATCTG AAAGCTGATC TTCAGAGGAA GGAAGAAGAT
    TATGCTGAGC TCAAAGAGAA ACTGGCTGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA
    GAGATCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG
    AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT
    AAGGAGCAGT TAAATAATCA AAAAGTGGAA GAAGCTATAC AACAGTATGA GAGAGTGTGC
    AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA
    GAGCAGACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA
    CTGGCCACAG AATTGGAAGA ATGGAAGGAA AAATACAAAG ATTTGGAAGC CAAACACAAT
    CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCAGTAAG
    CTTCAAGATG AGTTACAGGA ATCGGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA
    GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAATCTACG AAACAAAGAG
    ATGAAAAAAT ATGCCGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGA AATGGAAATA
    CTTAAAGGAC AGTTGGCTGA GAAAGATGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT
    CAACTTGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA
    GATAATGAAA TCGAACAATT AAAAAAGATC ACATCAGAGG CTTCTAAGAC AGAAAAACAG
    ACCATGGATA TCCACCTCAC ACAAATGAGT TTAACAGATC CTGGCAGGGT TGAAATTGAA
    CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT
    GACTCTTGTG AAGTGTCGAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA
    GAGATTCAAT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC
    TCACCAGTGA CGGTTAAGAT TCCCAAGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG
    GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT
    GTTTCTGAGC ATAGAAATTC TTTCAAAAAG GAACAAAAGG TTTCCATACG CCCATCATCT
    AAGAAAACTT ATTCTTTACG GAGTCATGCA TCCACAGTTG GTGTAAACGG GTCTGCTAAG
    AAAAAAGAAG GAACGCTACA GAAATTTGGA GACTTCTTAC AACATTCTCC AACTATTCTT
    CAGTCAAAAG CAAAGAAGAT AATTGAAACA ATGAGCTCTT CAAAGTTCTC AAATGTAGAA
    GTAAGTAAAG AAAATGTGTC TCGACCAAAA CGGGCCAAAC GAAAATTATA CACAAATGAA
    ATTTCATCTC CTATTGATAT ATCTGGCCAA GTGATTTTGA TGGACCGTAA AGTGAAAGAA
    AGCGATCATC AGATTCTCAA ACGACGACTT CGAACCAGAA CAGTCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49054
    Clone ID Related Accession (Same CDS sequence) XM_010819733.2
    Accession Version XM_010819733.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF20B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104580.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
    CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA
    TTTTCCTTAG TTGCTTCAAG CACTGAGGCA AACAGTTCTG AATCTAAAGA TTATCTCCAA
    GTTTGTCTTC GAGTAAGACC ATTCACACAG CCAGAAAAAG AACATGAGTC TGAGGGCTGT
    GTGTATATTC TGGATTCACA GACTGTTGTC CTGAAGGATC CTCAGTATAC TACCCTTGGT
    CGTTTAAGTG AGAAAAGCTC TGGGCAGATG GCACAAAAAT TCAGTTTTTC CAAGGTTTTT
    GGCCCAGAAA CTACACAGAA GGAATTCTTC CAGGGTTGCA TTCTGCAGCC AGTTAAAGAC
    CTCTTGAAAG GACAGAGTCG GCTGATTTTT ACTTACGGGC TAACCAATTC TGGAAAAACT
    TACACGTTTC AAGGCACAGA AGAAAATACT GGCATTCTTC CTCGAACTTT GAATGTATTA
    TTTGATAGTC TTCAGGAAAG ACTGTATACA AAGATGAACC TTAAACCACA TAGGTCCAGA
    GAATACTTAC GGTTATCACC AGACCAAGAG AAAGAAGAAG TCGCTAGCAA AAGTGCATTA
    CTTCGGCAAA TTAAAGAGGT TGTTATGCAT AATGACAGTT ATGGTGCTCT TTATGGAAGT
    CTAACAAACT CTTTGAGTAT CCCAGAGTTT GAAGAATCCA TGAAAGATTG TGAACACTCA
    AACTTGAACA TAGATATTAA TAGAAAATTT TCTGTGTGGG TATCTTTCTT TGAGATTTAC
    AACGAATGTA TTTACGATTT GTTTGTTCCT GTATCATCTA AGTTCCAAAA GAGAAAGACA
    CTGCGCCTTT CCCAAGATGT AAAGGGCTAT TCTTTCATAA AAGATCTACA GTGGATTCAA
    GTATCTGATG CCAAAGAAGC ATATAGACTT TTAAAGCTAG GAACAAAGCA CCAGAGTGTT
    GCTTTCACAA AACTGAACAA TGCTTCTAGT AGAAGTCACA GCATATTTAC CATTAGAATA
    TTACAGATTG AAGATTCAGA AATACCACAT GTAATACGAG TCAGTGAATT GTCTTTATGT
    GATCTTGCTG GCTCAGAACG AAGCATGAGG ACACAGAATG AAGGTGAAAG GTTAAGAGAG
    ACCGGGAATA TCAACACTTC TTTATTGACT CTGGGAAAGT GTATCAGTGT TTTGAAGAAT
    AGCGAAAAGT CAAAGTTTCA ACAGCATGTG CCTTTCCGGG AAAGTAAATT GACCCACTAT
    TTTCAAAGTT TTTTTAATGG TAAAGGGAAG ATATGTATGA TTGTCAACAT TAGCCAATGT
    TCTTTTACCT ATGATGAGAC ACTCAATGTG TTGAAGTTCT CAGCCATTGC ACAAAAAGTT
    TATGTTCCAG ATATTTTAAA TTGCTCTCAA GAGAAATCAT TTGGACCTGT CAAATCTTCT
    CAAGATGAAT CATTAGATAT TAATAATTCA GATAATAAAA TACTAAATGA AAAAAGATCC
    ACAATTTCAT GGGAAAGTAG CCTGGAAGAT GTGGTGGAAG ATGAAGATTT GGTTGAGGAT
    CTAGAAAAAG CTGAAGAAAA GCAGAATGTG GAAACTGAAT TTATTGATGA AGATCTACAT
    GATACATTAG AGGATGATAA GGCTTTTAGT AGCCATGCAG GGAAGAGAAA ACTTCTGGAT
    TTAATAGAAG ATTTGAAAAA AAAACTAATA AATGAAAGAA AGGAAAAGTT AACATTGGAA
    TTTAAAATTC GTGACGAAGT TACACAGGAG TTTACTCAGT ATTTGGCCCA ACGAGAAGCT
    GACTTTAAGG AAACTCTCCT TCAGGAACGA GAAATTTTAG AAGAAAATGC TGAATGTCGT
    TTGGCTATTT TCAAGGATTT GATTGGTAAA TGTGATGCTC AGGAGGAACC AAAGAATGAA
    GATCATGCCA TAAAAGATGA AACTGAAGAA GGACATAATT ATGTAGGAAT TGAAGATATT
    ATTGATTCTC TTCAAGATGA TGTCACTGGT ATTAAGAAAC AGGCTGAAAT TGCTCACTTA
    TATATTGCAT CTCTTGCTGA CCCCCAGGAA GCTATTGCTT GTTTAGAAAT AAAGTTTAAT
    CAAGTTAAAG CTGAATTAGC TAAAACCAAA GAAGAATTAA TCAAAACCCG AGAAGAGTTA
    AAAAAGAGAG AAAGTGAATC AGAAATTAAT TTAAATTCAT TGGTTCAAGA GCTTGAGAAA
    TCTAATAAGA AAATAATTAT GCAGAATCAA AGAATTCAAG AGTTGATGGA TAAAATTGAC
    CAAAAAGAGG ATACTATTAA CAAATTTCAG AACCTAAAAT TTCATATGGA AAACACATGT
    AAAGGCAGTG ACAATGTTGG TAATTCTTCT TTAATAATAA ACAGTAAATT GGTTTGTGAT
    GAAACGACTG AAATGTCTAA GGACAGCAAA ACTAAAACGT ATTCAGGAAG AAAAAGATTA
    AATGAAAACG AACTTCAACA AGATGAACCA CCAGCAAAGA AAGGACTTAC GCATGCTAGT
    CCAGCTATCA CTGAAGACCA AAAGAAAAGT GAAGAAATGC AAGAGAGCAT TTCAGATGAG
    GAAAACATTA GACTTCTACA AGAAAATAAT GAAGAGCTAA AAACACGTTT ACTCACCATT
    GAGAATGAAC TTAAAAATGA AAAGGAAGAA AAAACAGAAT TAAATAAACA GATTGTTAGT
    TTGCAGCAGG AACTTTCTTC TTCTGAAAAA AAAAGTTTAG GCTTTAGTAT AGAGATCCAA
    CAAATTCAGT CGAGTTATGA CAATGTGATT TCTGAATTAC ATGTGCAGAA AAGTATAAAT
    CAAGAACAGA AGGAAAGGAT CATGAAATTG TCAGAGGAGA TAGAAACTGC TAGACGAAAC
    ATCACAAACA ATGTTTCACA AATAAAATTA ATGCAAGCAA AAATAGATGA ACTGCGTAGA
    CTTGATTCAG TTTCTCAGAT CGCAAACATA GATTTACTCA ATCTCAGGGA TCTGTCCAGT
    TGTTCTCAAG AAGATAATTT GCCAAACACA CAGTTACATC ATTTGGATAA TGATTGTTTG
    ATAAGTAAGC AGGTTAAAGA ACATGGTATT CAAGAACTTA GTAGGGAAAG GTCTTTCCAC
    TCTACTGTTG AAGCCATTTG GGAAGAATGT AAAGAGATTG TAAAGACTTC CTCCAAAAAA
    ACCCATCAGA TTGAGGAACT AGAACAACAA ATTGAAAAAT TACAGGCAGA AGTAAAAGAC
    TATACAAATG AAAACAATAG ACTAAAAATA GAGGAGAGGG AGAAAAAGAA TCAAGATGAT
    TTACTAAAAG AAAAAGATAG TCTTATACAG CAGCTGAAGG AAGAAGTGCA AGAAAAAACC
    ATTAGTCTTG ATATTGAAGT ACAGCATGTA GTGGAAGGAA AAAGAGCACT TTCAGAACTT
    ATGCAAGATG TTACTTGCTA TAAGGTGAAA ATAAAGGAAC TTGAAGCAAT GTTAGAAACT
    CAGAAAGATG AATGTAGCCA TTCAGCCAAG TTAGAACAAG AAATTATGGA AAAGGAATCT
    ATCATTTTAA AGCAAGAAAG AAATCTGAAT GAATGTCAAG CAAATCTTAA AGATTCTATC
    CAAAATGCCA GAGATTTAAG TGAAAGGGAG GTCAAATTGA AAGAAGAAAT CATGCAATTA
    ACAAAAAATT TGCAAGATGC TAAGCAATCA CTTCAATTAA AGGAAGAAGA AAAGGAAAGA
    AACTGGCAAG AAACAGAAAA GTTGAAAGAG GAGCTCTCTG CAAGCACTAC TCTTACCCAG
    AATCTGAAAG CTGATCTTCA GAGGAAGGAA GAAGATTATG CTGAGCTCAA AGAGAAACTG
    GCTGATGCCA AAAAGCAGAT TGAGCAAGTA CAGAAAGAGA TCTCTGTAAT GCGTGATGAG
    GAGAAATTGT TGAGGATTAA AATTAATGAA CTGGAGAAAA AGAAAAACCA GTGTTCTCAG
    GAAATAGATA TGAAACAGCG AACCATTCAG CAACTTAAGG AGCAGTTAAA TAATCAAAAA
    GTGGAAGAAG CTATACAACA GTATGAGAGA GTGTGCAAAG ATCTGAGTGT TAAAGAAAAA
    ATAATCAAAG ACATGCAAAT GACCCTGGAA GAACAAGAGC AGACTCAGGT AGAACAAGAT
    CAAGTGCTTG AGGCTAAATT AGAGGAAACC GAAAGACTGG CCACAGAATT GGAAGAATGG
    AAGGAAAAAT ACAAAGATTT GGAAGCCAAA CACAATCAAA GTTCAAATAA AGAATTTGAG
    GATAACACAG ATGTACTTAA TACAAAACTC AGTAAGCTTC AAGATGAGTT ACAGGAATCG
    GAACAGAAAC ATGAAGCTGA AAGAAAAAAA TGGTTAGAAG AAAAAATGAT GCTTATCACT
    CAAGCAAAAG AAGCTGAGAA TCTACGAAAC AAAGAGATGA AAAAATATGC CGAAGACAGA
    GAGCATTGTT TGAAGCAACA AAATGAAATG GAAATACTTA AAGGACAGTT GGCTGAGAAA
    GATGGTAACC TCCAGCAGTG GCGGGAAGAA CGCGATCAAC TTGTTGCAGC TTTAGAAATA
    CAGCTGAAAG CACTGATCTC CAGTAATGTA CAAAAAGATA ATGAAATCGA ACAATTAAAA
    AAGATCACAT CAGAGGCTTC TAAGACAGAA AAACAGACCA TGGATATCCA CCTCACACAA
    ATGAGTTTAA CAGATCCTGG CAGGGTTGAA ATTGAACCTC AGTCAACAAG TTTTGAAATT
    TCCAGAGGTG AAGTAGAGGA TGGATCTGTA GTCCTTGACT CTTGTGAAGT GTCGACAGAA
    AATAATCAAA GCACTCGATT TCCAAAACCT GAGTTAGAGA TTCAATTTAC ACCTTTACAG
    CCAAACAGAA TGGCAGTGAA ACACCCAGGT TGTACCTCAC CAGTGACGGT TAAGATTCCC
    AAGGCCCGAA AGAGGAAAAG TCATGAAATG GAAGAGGACT TTGTGAAATT TGAAAATAAG
    AAGAATGCTA CACCAAGAAT TAATTTGAAG TCCCCTGTTT CTGAGCATAG AAATTCTTTC
    AAAAAGGAAC AAAAGGTTTC CATACGCCCA TCATCTAAGA AAACTTATTC TTTACGGAGT
    CATGCATCCA CAGTTGGTGT AAACGGGTCT GCTAAGAAAA AAGAAGGAAC GCTACAGAAA
    TTTGGAGACT TCTTACAACA TTCTCCAACT ATTCTTCAGT CAAAAGCAAA GAAGATAATT
    GAAACAATGA GCTCTTCAAA GTTCTCAAAT GTAGAAGTAA GTAAAGAAAA TGTGTCTCGA
    CCAAAACGGG CCAAACGAAA ATTATACACA AATGAAATTT CATCTCCTAT TGATATATCT
    GGCCAAGTGA TTTTGATGGA CCGTAAAGTG AAAGAAAGCG ATCATCAGAT TCTCAAACGA
    CGACTTCGAA CCAGAACAGT CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818035.1
    CDS112..5598
    Translation

    Target ORF information:

    RefSeq Version XM_010819733.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 20B (KIF20B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819733.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
    CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA
    TTTTCCTTAG TTGCTTCAAG CACTGAGGCA AACAGTTCTG AATCTAAAGA TTATCTCCAA
    GTTTGTCTTC GAGTAAGACC ATTCACACAG CCAGAAAAAG AACATGAGTC TGAGGGCTGT
    GTGTATATTC TGGATTCACA GACTGTTGTC CTGAAGGATC CTCAGTATAC TACCCTTGGT
    CGTTTAAGTG AGAAAAGCTC TGGGCAGATG GCACAAAAAT TCAGTTTTTC CAAGGTTTTT
    GGCCCAGAAA CTACACAGAA GGAATTCTTC CAGGGTTGCA TTCTGCAGCC AGTTAAAGAC
    CTCTTGAAAG GACAGAGTCG GCTGATTTTT ACTTACGGGC TAACCAATTC TGGAAAAACT
    TACACGTTTC AAGGCACAGA AGAAAATACT GGCATTCTTC CTCGAACTTT GAATGTATTA
    TTTGATAGTC TTCAGGAAAG ACTGTATACA AAGATGAACC TTAAACCACA TAGGTCCAGA
    GAATACTTAC GGTTATCACC AGACCAAGAG AAAGAAGAAG TCGCTAGCAA AAGTGCATTA
    CTTCGGCAAA TTAAAGAGGT TGTTATGCAT AATGACAGTT ATGGTGCTCT TTATGGAAGT
    CTAACAAACT CTTTGAGTAT CCCAGAGTTT GAAGAATCCA TGAAAGATTG TGAACACTCA
    AACTTGAACA TAGATATTAA TAGAAAATTT TCTGTGTGGG TATCTTTCTT TGAGATTTAC
    AACGAATGTA TTTACGATTT GTTTGTTCCT GTATCATCTA AGTTCCAAAA GAGAAAGACA
    CTGCGCCTTT CCCAAGATGT AAAGGGCTAT TCTTTCATAA AAGATCTACA GTGGATTCAA
    GTATCTGATG CCAAAGAAGC ATATAGACTT TTAAAGCTAG GAACAAAGCA CCAGAGTGTT
    GCTTTCACAA AACTGAACAA TGCTTCTAGT AGAAGTCACA GCATATTTAC CATTAGAATA
    TTACAGATTG AAGATTCAGA AATACCACAT GTAATACGAG TCAGTGAATT GTCTTTATGT
    GATCTTGCTG GCTCAGAACG AAGCATGAGG ACACAGAATG AAGGTGAAAG GTTAAGAGAG
    ACCGGGAATA TCAACACTTC TTTATTGACT CTGGGAAAGT GTATCAGTGT TTTGAAGAAT
    AGCGAAAAGT CAAAGTTTCA ACAGCATGTG CCTTTCCGGG AAAGTAAATT GACCCACTAT
    TTTCAAAGTT TTTTTAATGG TAAAGGGAAG ATATGTATGA TTGTCAACAT TAGCCAATGT
    TCTTTTACCT ATGATGAGAC ACTCAATGTG TTGAAGTTCT CAGCCATTGC ACAAAAAGTT
    TATGTTCCAG ATATTTTAAA TTGCTCTCAA GAGAAATCAT TTGGACCTGT CAAATCTTCT
    CAAGATGAAT CATTAGATAT TAATAATTCA GATAATAAAA TACTAAATGA AAAAAGATCC
    ACAATTTCAT GGGAAAGTAG CCTGGAAGAT GTGGTGGAAG ATGAAGATTT GGTTGAGGAT
    CTAGAAAAAG CTGAAGAAAA GCAGAATGTG GAAACTGAAT TTATTGATGA AGATCTACAT
    GATACATTAG AGGATGATAA GGCTTTTAGT AGCCATGCAG GGAAGAGAAA ACTTCTGGAT
    TTAATAGAAG ATTTGAAAAA AAAACTAATA AATGAAAGAA AGGAAAAGTT AACATTGGAA
    TTTAAAATTC GTGACGAAGT TACACAGGAG TTTACTCAGT ATTTGGCCCA ACGAGAAGCT
    GACTTTAAGG AAACTCTCCT TCAGGAACGA GAAATTTTAG AAGAAAATGC TGAATGTCGT
    TTGGCTATTT TCAAGGATTT GATTGGTAAA TGTGATGCTC AGGAGGAACC AAAGAATGAA
    GATCATGCCA TAAAAGATGA AACTGAAGAA GGACATAATT ATGTAGGAAT TGAAGATATT
    ATTGATTCTC TTCAAGATGA TGTCACTGGT ATTAAGAAAC AGGCTGAAAT TGCTCACTTA
    TATATTGCAT CTCTTGCTGA CCCCCAGGAA GCTATTGCTT GTTTAGAAAT AAAGTTTAAT
    CAAGTTAAAG CTGAATTAGC TAAAACCAAA GAAGAATTAA TCAAAACCCG AGAAGAGTTA
    AAAAAGAGAG AAAGTGAATC AGAAATTAAT TTAAATTCAT TGGTTCAAGA GCTTGAGAAA
    TCTAATAAGA AAATAATTAT GCAGAATCAA AGAATTCAAG AGTTGATGGA TAAAATTGAC
    CAAAAAGAGG ATACTATTAA CAAATTTCAG AACCTAAAAT TTCATATGGA AAACACATGT
    AAAGGCAGTG ACAATGTTGG TAATTCTTCT TTAATAATAA ACAGTAAATT GGTTTGTGAT
    GAAACGACTG AAATGTCTAA GGACAGCAAA ACTAAAACGT ATTCAGGAAG AAAAAGATTA
    AATGAAAACG AACTTCAACA AGATGAACCA CCAGCAAAGA AAGGACTTAC GCATGCTAGT
    CCAGCTATCA CTGAAGACCA AAAGAAAAGT GAAGAAATGC AAGAGAGCAT TTCAGATGAG
    GAAAACATTA GACTTCTACA AGAAAATAAT GAAGAGCTAA AAACACGTTT ACTCACCATT
    GAGAATGAAC TTAAAAATGA AAAGGAAGAA AAAACAGAAT TAAATAAACA GATTGTTAGT
    TTGCAGCAGG AACTTTCTTC TTCTGAAAAA AAAAGTTTAG GCTTTAGTAT AGAGATCCAA
    CAAATTCAGT CGAGTTATGA CAATGTGATT TCTGAATTAC ATGTGCAGAA AAGTATAAAT
    CAAGAACAGA AGGAAAGGAT CATGAAATTG TCAGAGGAGA TAGAAACTGC TAGACGAAAC
    ATCACAAACA ATGTTTCACA AATAAAATTA ATGCAAGCAA AAATAGATGA ACTGCGTAGA
    CTTGATTCAG TTTCTCAGAT CGCAAACATA GATTTACTCA ATCTCAGGGA TCTGTCCAGT
    TGTTCTCAAG AAGATAATTT GCCAAACACA CAGTTACATC ATTTGGATAA TGATTGTTTG
    ATAAGTAAGC AGGTTAAAGA ACATGGTATT CAAGAACTTA GTAGGGAAAG GTCTTTCCAC
    TCTACTGTTG AAGCCATTTG GGAAGAATGT AAAGAGATTG TAAAGACTTC CTCCAAAAAA
    ACCCATCAGA TTGAGGAACT AGAACAACAA ATTGAAAAAT TACAGGCAGA AGTAAAAGAC
    TATACAAATG AAAACAATAG ACTAAAAATA GAGGAGAGGG AGAAAAAGAA TCAAGATGAT
    TTACTAAAAG AAAAAGATAG TCTTATACAG CAGCTGAAGG AAGAAGTGCA AGAAAAAACC
    ATTAGTCTTG ATATTGAAGT ACAGCATGTA GTGGAAGGAA AAAGAGCACT TTCAGAACTT
    ATGCAAGATG TTACTTGCTA TAAGGTGAAA ATAAAGGAAC TTGAAGCAAT GTTAGAAACT
    CAGAAAGATG AATGTAGCCA TTCAGCCAAG TTAGAACAAG AAATTATGGA AAAGGAATCT
    ATCATTTTAA AGCAAGAAAG AAATCTGAAT GAATGTCAAG CAAATCTTAA AGATTCTATC
    CAAAATGCCA GAGATTTAAG TGAAAGGGAG GTCAAATTGA AAGAAGAAAT CATGCAATTA
    ACAAAAAATT TGCAAGATGC TAAGCAATCA CTTCAATTAA AGGAAGAAGA AAAGGAAAGA
    AACTGGCAAG AAACAGAAAA GTTGAAAGAG GAGCTCTCTG CAAGCACTAC TCTTACCCAG
    AATCTGAAAG CTGATCTTCA GAGGAAGGAA GAAGATTATG CTGAGCTCAA AGAGAAACTG
    GCTGATGCCA AAAAGCAGAT TGAGCAAGTA CAGAAAGAGA TCTCTGTAAT GCGTGATGAG
    GAGAAATTGT TGAGGATTAA AATTAATGAA CTGGAGAAAA AGAAAAACCA GTGTTCTCAG
    GAAATAGATA TGAAACAGCG AACCATTCAG CAACTTAAGG AGCAGTTAAA TAATCAAAAA
    GTGGAAGAAG CTATACAACA GTATGAGAGA GTGTGCAAAG ATCTGAGTGT TAAAGAAAAA
    ATAATCAAAG ACATGCAAAT GACCCTGGAA GAACAAGAGC AGACTCAGGT AGAACAAGAT
    CAAGTGCTTG AGGCTAAATT AGAGGAAACC GAAAGACTGG CCACAGAATT GGAAGAATGG
    AAGGAAAAAT ACAAAGATTT GGAAGCCAAA CACAATCAAA GTTCAAATAA AGAATTTGAG
    GATAACACAG ATGTACTTAA TACAAAACTC AGTAAGCTTC AAGATGAGTT ACAGGAATCG
    GAACAGAAAC ATGAAGCTGA AAGAAAAAAA TGGTTAGAAG AAAAAATGAT GCTTATCACT
    CAAGCAAAAG AAGCTGAGAA TCTACGAAAC AAAGAGATGA AAAAATATGC CGAAGACAGA
    GAGCATTGTT TGAAGCAACA AAATGAAATG GAAATACTTA AAGGACAGTT GGCTGAGAAA
    GATGGTAACC TCCAGCAGTG GCGGGAAGAA CGCGATCAAC TTGTTGCAGC TTTAGAAATA
    CAGCTGAAAG CACTGATCTC CAGTAATGTA CAAAAAGATA ATGAAATCGA ACAATTAAAA
    AAGATCACAT CAGAGGCTTC TAAGACAGAA AAACAGACCA TGGATATCCA CCTCACACAA
    ATGAGTTTAA CAGATCCTGG CAGGGTTGAA ATTGAACCTC AGTCAACAAG TTTTGAAATT
    TCCAGAGGTG AAGTAGAGGA TGGATCTGTA GTCCTTGACT CTTGTGAAGT GTCGACAGAA
    AATAATCAAA GCACTCGATT TCCAAAACCT GAGTTAGAGA TTCAATTTAC ACCTTTACAG
    CCAAACAGAA TGGCAGTGAA ACACCCAGGT TGTACCTCAC CAGTGACGGT TAAGATTCCC
    AAGGCCCGAA AGAGGAAAAG TCATGAAATG GAAGAGGACT TTGTGAAATT TGAAAATAAG
    AAGAATGCTA CACCAAGAAT TAATTTGAAG TCCCCTGTTT CTGAGCATAG AAATTCTTTC
    AAAAAGGAAC AAAAGGTTTC CATACGCCCA TCATCTAAGA AAACTTATTC TTTACGGAGT
    CATGCATCCA CAGTTGGTGT AAACGGGTCT GCTAAGAAAA AAGAAGGAAC GCTACAGAAA
    TTTGGAGACT TCTTACAACA TTCTCCAACT ATTCTTCAGT CAAAAGCAAA GAAGATAATT
    GAAACAATGA GCTCTTCAAA GTTCTCAAAT GTAGAAGTAA GTAAAGAAAA TGTGTCTCGA
    CCAAAACGGG CCAAACGAAA ATTATACACA AATGAAATTT CATCTCCTAT TGATATATCT
    GGCCAAGTGA TTTTGATGGA CCGTAAAGTG AAAGAAAGCG ATCATCAGAT TCTCAAACGA
    CGACTTCGAA CCAGAACAGT CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.