Gpx2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpx2
Entrez Gene ID	29326
Full Name	glutathione peroxidase 2
Synonyms	GPX-GI,GSHPx-2,GSHPx-GI
General protein information	Preferred Names glutathione peroxidase 2 Names glutathione peroxidase 2 GPx-2 glutathione peroxidase-gastrointestinal
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24
Summary	The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is predominantly expressed in the gastrointestinal tract in rodents, is localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. Knockout studies in mice lacking this gene suggest a role for this isozyme in intestinal inflammation and colon cancer development. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Pseudogenes of this locus have been identified on chromosomes 2 and X. [provided by RefSeq, Aug 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_183403.2	NP_899653.2	glutathione peroxidase 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gpx2	NP_899653.2
Homo sapiens (human)	GPX2	NP_002074.2
Gallus gallus (chicken)	GPX2	NP_001264783.1
Bos taurus (cattle)	GPX2	NP_001156611.1
Xenopus tropicalis (tropical clawed frog)	gpx2	NP_001243244.1
Mus musculus (house mouse)	Gpx2	NP_109602.2
Pan troglodytes (chimpanzee)	GPX2	NP_001108606.1
	GPX2	NP_001108609.1
Canis lupus familiaris (dog)	GPX2	NP_001108607.1

Gene Ontology
Bibliography

Related articles in PubMed

Novel candidate genes for impaired nephron development in a rat model with inherited nephron deficit and albuminuria.
Herlan L, Schulz A, Schulte L, Schulz H, H?bner N, Kreutz R
Clinical and experimental pharmacology & physiology42(10)1051-8(2015 Oct)

Selenium-enriched Agaricus bisporus mushroom protects against increase in gut permeability ex vivo and up-regulates glutathione peroxidase 1 and 2 in hyperthermally-induced oxidative stress in rats.
Maseko T, Dunshea FR, Howell K, Cho HJ, Rivera LR, Furness JB, Ng K
Nutrients6(6)2478-92(2014 Jun)

Expression of glutathione peroxidase 2 is associated with not only early hepatocarcinogenesis but also late stage metastasis.
Suzuki S, Pitchakarn P, Ogawa K, Naiki-Ito A, Chewonarin T, Punfa W, Asamoto M, Shirai T, Takahashi S
Toxicology311(3)115-23(2013 Sep)

Gpx2 is an overexpressed gene in rat breast cancers induced by three different chemical carcinogens.
Naiki-Ito A, Asamoto M, Hokaiwado N, Takahashi S, Yamashita H, Tsuda H, Ogawa K, Shirai T
Cancer research67(23)11353-8(2007 Dec)

Activity of the glutathione peroxidase-2. Differences in the selenium-dependent expression between colon and small intestine.
Bartel J, Bartz T, Wolf C, Charkiewicz E, Kuhbacher M, Pohl T, Kyriakopoulos A
Cancer genomics & proteomics4(5)369-72(2007 Sep-Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dietary Se-enriched A. bisporus protected against increase in ileum permeability and up-regulated GPx-1 and GPx-2 expression, selenoenzymes relevant to mitigating oxidative stress.
Title: Selenium-enriched Agaricus bisporus mushroom protects against increase in gut permeability ex vivo and up-regulates glutathione peroxidase 1 and 2 in hyperthermally-induced oxidative stress in rats.

Expression of glutathione peroxidase 2 is associated with not only early hepatocarcinogenesis but also late stage metastasis
Title: Expression of glutathione peroxidase 2 is associated with not only early hepatocarcinogenesis but also late stage metastasis.

Gpx2 is an overexpressed gene in rat breast cancers
Title: Gpx2 is an overexpressed gene in rat breast cancers induced by three different chemical carcinogens.

Selenoenzyme GPx-2 is predominantly expressed in the tissues of the gastrointestinal tract, such as the small intestine and colon.
Title: Activity of the glutathione peroxidase-2. Differences in the selenium-dependent expression between colon and small intestine.

Novel sites of Gpx2 expression were observed in colon lymphatic tissue.
Title: Novel sites of cytosolic glutathione peroxidase expression in colon.

The following Gpx2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpx2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12803	NM_183403.2 Latest version!	Rattus norvegicus glutathione peroxidase 2 (Gpx2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpx2 ( NM_183403.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_899653.2 Rattus norvegicus glutathione peroxidase 2 (Gpx2), mRNA.

Price & Availability↑

CloneID	ORa12803
Clone ID Related Accession (Same CDS sequence)	NM_183403.2
Accession Version	NM_183403.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	glutathione peroxidase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG664050.1, CX570255.1 and BQ196565.1. On Apr 13, 2007 this sequence version replaced NM_183403.1. ##Evidence-Data-START## Transcript exon combination :: FQ220474.1, CX570255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTTACA TCGCCAAGTC TTTTTACGAT CTCAGTGCCA TCGGCCTGGA TGGGGAGAAG 
ATAGACTTCA ACACGTTCCG AGGCAGGGCC GTGCTGATTG AGAATGTGGC CTCGCTCTGA 
GGAACAACTA CCCGGGACTA CACCCAGCTC AATGAGTTGC AGTGCCGCTT TCCCAGGCGC 
CTAGTGGTTC TCGGCTTCCC TTGCAACCAG TTCGGACATC AGGAGAACTG TCAGAATGAG 
GAGATCCTGA ACAGCCTCAA GTATGTCCGC CCTGGGGGTG GGTTCCAGCC CACCTTCAGT 
CTTACCCAAA AGTGTGATGT CAATGGGCAG AATCAGCATC CTGTCTTTGC CTACCTGAAA 
GACAAGCTGC CCTACCCTTA TGACGACCCA TTCTCCCTCA TGACCGATCC CAAGCTCATC 
ATATGGAGTC CGGTGCGCCG CTCAGATGTG TCCTGGAACT TTGAGAAGTT CCTCATAGGG 
CCAGAAGGGG AGCCTTTCCG TCGCTACAGC CGCACCTTCC AGACCATCAA CATCGAGCCT 
GACATCAAAC GTCTCCTCAA AGTTGCCATC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899653.2
CDS	122..694
Translation	MAYIAKSFYDLSAIGLDGEKIDFNTFRGRAVLIENVASL*GTTTRDYTQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGFQPTFSLTQKCDVNGQNQHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRTFQTINIEPDIKRLLKVAI

Target ORF information:

RefSeq Version	NM_183403.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutathione peroxidase 2 (Gpx2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183403.2

ORF Insert Sequence:

ATGGCTTACA TCGCCAAGTC TTTTTACGAT CTCAGTGCCA TCGGCCTGGA TGGGGAGAAG 
ATAGACTTCA ACACGTTCCG AGGCAGGGCC GTGCTGATTG AGAATGTGGC CTCGCTCTGA 
GGAACAACTA CCCGGGACTA CACCCAGCTC AATGAGTTGC AGTGCCGCTT TCCCAGGCGC 
CTAGTGGTTC TCGGCTTCCC TTGCAACCAG TTCGGACATC AGGAGAACTG TCAGAATGAG 
GAGATCCTGA ACAGCCTCAA GTATGTCCGC CCTGGGGGTG GGTTCCAGCC CACCTTCAGT 
CTTACCCAAA AGTGTGATGT CAATGGGCAG AATCAGCATC CTGTCTTTGC CTACCTGAAA 
GACAAGCTGC CCTACCCTTA TGACGACCCA TTCTCCCTCA TGACCGATCC CAAGCTCATC 
ATATGGAGTC CGGTGCGCCG CTCAGATGTG TCCTGGAACT TTGAGAAGTT CCTCATAGGG 
CCAGAAGGGG AGCCTTTCCG TCGCTACAGC CGCACCTTCC AGACCATCAA CATCGAGCCT 
GACATCAAAC GTCTCCTCAA AGTTGCCATC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel candidate genes for impaired nephron development in a rat model with inherited nephron deficit and albuminuria.
Clinical and experimental pharmacology & physiology42(10)1051-8(2015 Oct)
Herlan L,Schulz A,Schulte L,Schulz H,H?bner N,Kreutz R

Selenium-enriched Agaricus bisporus mushroom protects against increase in gut permeability ex vivo and up-regulates glutathione peroxidase 1 and 2 in hyperthermally-induced oxidative stress in rats.
Nutrients6(6)2478-92(2014 Jun)
Maseko T,Dunshea FR,Howell K,Cho HJ,Rivera LR,Furness JB,Ng K

Expression of glutathione peroxidase 2 is associated with not only early hepatocarcinogenesis but also late stage metastasis.
Toxicology311(3)115-23(2013 Sep)
Suzuki S,Pitchakarn P,Ogawa K,Naiki-Ito A,Chewonarin T,Punfa W,Asamoto M,Shirai T,Takahashi S

Gpx2 is an overexpressed gene in rat breast cancers induced by three different chemical carcinogens.
Cancer research67(23)11353-8(2007 Dec)
Naiki-Ito A,Asamoto M,Hokaiwado N,Takahashi S,Yamashita H,Tsuda H,Ogawa K,Shirai T

Activity of the glutathione peroxidase-2. Differences in the selenium-dependent expression between colon and small intestine.
Cancer genomics & proteomics4(5)369-72(2007 Sep-Oct)
Bartel J,Bartz T,Wolf C,Charkiewicz E,Kuhbacher M,Pohl T,Kyriakopoulos A

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Gpx2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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