gpx2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	gpx2
Entrez Gene ID	100145152
Full Name	glutathione peroxidase 2
General protein information	Preferred Names glutathione peroxidase 2 Names glutathione peroxidase 2 glutathione peroxidase 2 (gastrointestinal)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)
Summary	The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is predominantly expressed in the gastrointestinal tract and localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. It is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256315.1	NP_001243244.1	glutathione peroxidase 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gpx2	NP_899653.2
Homo sapiens (human)	GPX2	NP_002074.2
Gallus gallus (chicken)	GPX2	NP_001264783.1
Bos taurus (cattle)	GPX2	NP_001156611.1
Xenopus tropicalis (tropical clawed frog)	gpx2	NP_001243244.1
Mus musculus (house mouse)	Gpx2	NP_109602.2
Pan troglodytes (chimpanzee)	GPX2	NP_001108606.1
	GPX2	NP_001108609.1
Canis lupus familiaris (dog)	GPX2	NP_001108607.1

Gene Ontology
Bibliography

Related articles in PubMed

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

Exploring nervous system transcriptomes during embryogenesis and metamorphosis in Xenopus tropicalis using EST analysis.
Fierro AC, Thuret R, Coen L, Perron M, Demeneix BA, Wegnez M, Gyapay G, Weissenbach J, Wincker P, Mazabraud A, Pollet N
BMC genomics8118(2007 May)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gpx2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gpx2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa25511	NM_001256315.1 Latest version!	Xenopus tropicalis glutathione peroxidase 2 (gpx2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gpx2 ( NM_001256315.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001243244.1 Xenopus tropicalis glutathione peroxidase 2 (gpx2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OXa25511
Clone ID Related Accession (Same CDS sequence)	NM_001256315.1
Accession Version	NM_001256315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	glutathione peroxidase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN103209.1, BX689060.1 and CU075479.1. On Jan 20, 2012 this sequence version replaced XR_097489.1. Transcript Variant: This variant (1) represents the protein-coding transcript. ##Evidence-Data-START## Transcript exon combination :: CU075479.1, BC159036.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTTACA TAGCAAAGTC ATTCTATGAC CTATATGCTA CAAACATAGA TGGGGAGAAA 
GTAGATTTCA ATGTGTTTAG GGGAAGGGTG GTGTTGATAG AAAATGTTGC TTCACTTTGA 
GACACCACAG TCAGGGATTA CACCCAGCTT AATGAGCTGC AGACCAAGTA TCCGCGACGG 
CTTGTGGTGC TGGGATTTCC ATGCAATCAA TTTGGTTATC AAGAAAACTG TAAAAATGAA 
GAGATTCTTA ATTCTTTAAA ATATGTACGA CCCGGAAAAG GCTTTGTACC AGGATTCACC 
CTGTTCCAGA AATGTGATGT TAATGGAAAA GACACTCATT CGGTTTTTGC TTACTTAAAG 
GACAAACTAC CAGTCCCTGA CAATGAGCCT GCAGCCCTTA TTAGTGACCC CAGATATATT 
GTATGGAATC CAGTACATCG GTCTGACATT TCCTGGAACT TTGAGAAGTT TCTTATTGGA 
CCTGAAGGAG AACCATTCAA AAGATACAAC AAAAATTTCC AAACCATCAG TATAGAACCT 
GACATCCAGA GACTTCTTAA GCTCACCAAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243244.1
CDS	33..605
Translation	MAYIAKSFYDLYATNIDGEKVDFNVFRGRVVLIENVASL*DTTVRDYTQLNELQTKYPRRLVVLGFPCNQFGYQENCKNEEILNSLKYVRPGKGFVPGFTLFQKCDVNGKDTHSVFAYLKDKLPVPDNEPAALISDPRYIVWNPVHRSDISWNFEKFLIGPEGEPFKRYNKNFQTISIEPDIQRLLKLTK

Target ORF information:

RefSeq Version	NM_001256315.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutathione peroxidase 2 (gpx2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256315.1

ORF Insert Sequence:

ATGGCTTACA TAGCAAAGTC ATTCTATGAC CTATATGCTA CAAACATAGA TGGGGAGAAA 
GTAGATTTCA ATGTGTTTAG GGGAAGGGTG GTGTTGATAG AAAATGTTGC TTCACTTTGA 
GACACCACAG TCAGGGATTA CACCCAGCTT AATGAGCTGC AGACCAAGTA TCCGCGACGG 
CTTGTGGTGC TGGGATTTCC ATGCAATCAA TTTGGTTATC AAGAAAACTG TAAAAATGAA 
GAGATTCTTA ATTCTTTAAA ATATGTACGA CCCGGAAAAG GCTTTGTACC AGGATTCACC 
CTGTTCCAGA AATGTGATGT TAATGGAAAA GACACTCATT CGGTTTTTGC TTACTTAAAG 
GACAAACTAC CAGTCCCTGA CAATGAGCCT GCAGCCCTTA TTAGTGACCC CAGATATATT 
GTATGGAATC CAGTACATCG GTCTGACATT TCCTGGAACT TTGAGAAGTT TCTTATTGGA 
CCTGAAGGAG AACCATTCAA AAGATACAAC AAAAATTTCC AAACCATCAG TATAGAACCT 
GACATCCAGA GACTTCTTAA GCTCACCAAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

Exploring nervous system transcriptomes during embryogenesis and metamorphosis in Xenopus tropicalis using EST analysis.
BMC genomics8118(2007 May)
Fierro AC,Thuret R,Coen L,Perron M,Demeneix BA,Wegnez M,Gyapay G,Weissenbach J,Wincker P,Mazabraud A,Pollet N

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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gpx2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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