Gpx2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpx2
Entrez Gene ID	14776
Full Name	glutathione peroxidase 2
Synonyms	GI-GPx,GPx-GI,GSHPx-2,GSHPx-GI
General protein information	Preferred Names glutathione peroxidase 2 Names glutathione peroxidase 2 GPx-2 glutathione peroxidase-gastrointestinal intestinal GPx
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 C3\|12 33.73 cM
Summary	The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is predominantly expressed in the gastrointestinal tract in rodents, is localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. Knockout studies in mice lacking this gene suggest a role for this isozyme in intestinal inflammation and colon cancer development. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A pseudogene of this locus has been identified on chromosome 7. [provided by RefSeq, Aug 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_030677.2	NP_109602.2	glutathione peroxidase 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gpx2	NP_899653.2
Homo sapiens (human)	GPX2	NP_002074.2
Gallus gallus (chicken)	GPX2	NP_001264783.1
Bos taurus (cattle)	GPX2	NP_001156611.1
Xenopus tropicalis (tropical clawed frog)	gpx2	NP_001243244.1
Mus musculus (house mouse)	Gpx2	NP_109602.2
Pan troglodytes (chimpanzee)	GPX2	NP_001108606.1
	GPX2	NP_001108609.1
Canis lupus familiaris (dog)	GPX2	NP_001108607.1

Gene Ontology
Bibliography

Related articles in PubMed

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
Wu Y, Wei FY, Kawarada L, Suzuki T, Araki K, Komohara Y, Fujimura A, Kaitsuka T, Takeya M, Oike Y, Suzuki T, Tomizawa K
PLoS genetics12(9)e1006355(2016 Sep)

Cold/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock-induced oxidation.
Borowiec AS, Sion B, Chalmel F, D Rolland A, Lemonnier L, De Clerck T, Bokhobza A, Derouiche S, Dewailly E, Slomianny C, Mauduit C, Benahmed M, Roudbaraki M, J?gou B, Prevarskaya N, Bidaux G
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(9)3155-70(2016 09)

Deletion of glutathione peroxidase-2 inhibits azoxymethane-induced colon cancer development.
M?ller MF, Florian S, Pommer S, Osterhoff M, Esworthy RS, Chu FF, Brigelius-Floh? R, Kipp AP
PloS one8(8)e72055(2013)

Expression of extracellular glutathione peroxidase in human and mouse gastrointestinal tract.
Tham DM, Whitin JC, Kim KK, Zhu SX, Cohen HJ
The American journal of physiology275(6 Pt 1)G1463-71(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High glutathione peroxidase 2 expression is associated with radiation induced lung disease.
Title: Basal levels of glutathione peroxidase correlate with onset of radiation induced lung disease in inbred mouse strains.

we propose that ileocolitis in the GPx1/2-double-knockout mice is caused by Nox1, which is induced by TNF
Title: Nox1 causes ileocolitis in mice deficient in glutathione peroxidase-1 and -2.

GPx2 is up-regulated in dysplastic colonic crypts, where it appears to act inhibit apoptosis and, thus, supports tumor development.
Title: Deletion of glutathione peroxidase-2 inhibits azoxymethane-induced colon cancer development.

it is concluded that GPx2, although supporting tumor growth, inhibits inflammation-mediated tumorigenesis, but the protective effect of selenium does not strictly depend on GPx2 expression
Title: Glutathione peroxidase-2 and selenium decreased inflammation and tumors in a mouse model of inflammation-associated carcinogenesis whereas sulforaphane effects differed with selenium supply.

Candidate colitis genes in the Gdac1 locus of mice deficient in glutathione peroxidase-1 and -2, were analyzed.
Title: Analysis of candidate colitis genes in the Gdac1 locus of mice deficient in glutathione peroxidase-1 and -2.

The following Gpx2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpx2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17757	NM_030677.2 Latest version!	Mus musculus glutathione peroxidase 2 (Gpx2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpx2 ( NM_030677.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_109602.2 Mus musculus glutathione peroxidase 2 (Gpx2), mRNA.

Price & Availability↑

CloneID	OMu17757
Clone ID Related Accession (Same CDS sequence)	NM_030677.2
Accession Version	NM_030677.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	glutathione peroxidase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CF612186.1, AK131940.1 and BX629737.1. On Apr 13, 2007 this sequence version replaced NM_030677.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK131940.1, BC039658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 9637305 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTTACA TTGCCAAGTC GTTCTACGAT CTCAGTGCCG TTGGCCTGGA TGGGGAGAAG 
ATAGACTTCA ATACGTTCAG AGGCAGGGCT GTGCTGATTG AGAATGTGGC GTCACTCTGA 
GGAACAACTA CCCGGGACTA CAACCAGCTC AATGAGCTGC AATGTCGCTT TCCCAGGCGC 
CTGGTAGTTC TCGGCTTCCC TTGCAACCAG TTCGGACATC AGGAGAACTG TCAGAACGAG 
GAGATCCTGA ACAGCCTCAA GTATGTCCGA CCTGGGGGTG GGTACCAGCC CACCTTTAGT 
CTTACCCAAA AGTGTGACGT CAATGGGCAG AACGAGCATC CTGTCTTTGC CTACCTGAAA 
GACAAGCTGC CCTACCCTTA TGATGACCCG TTCTCCCTCA TGACCGATCC CAAGCTCATC 
ATATGGAGTC CCGTGCGCCG CTCAGACGTG TCCTGGAACT TTGAGAAGTT CCTCATAGGG 
CCAGAAGGGG AGCCCTTCCG TCGCTACAGC CGCAGCTTCC AGACCATCAA CATCGAGCCT 
GACATCAAAC GGCTCCTCAA AGTTGCCATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_109602.2
CDS	180..752
Translation	MAYIAKSFYDLSAVGLDGEKIDFNTFRGRAVLIENVASL*GTTTRDYNQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFSLTQKCDVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRSFQTINIEPDIKRLLKVAI

Target ORF information:

RefSeq Version	NM_030677.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutathione peroxidase 2 (Gpx2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030677.2

ORF Insert Sequence:

ATGGCTTACA TTGCCAAGTC GTTCTACGAT CTCAGTGCCG TTGGCCTGGA TGGGGAGAAG 
ATAGACTTCA ATACGTTCAG AGGCAGGGCT GTGCTGATTG AGAATGTGGC GTCACTCTGA 
GGAACAACTA CCCGGGACTA CAACCAGCTC AATGAGCTGC AATGTCGCTT TCCCAGGCGC 
CTGGTAGTTC TCGGCTTCCC TTGCAACCAG TTCGGACATC AGGAGAACTG TCAGAACGAG 
GAGATCCTGA ACAGCCTCAA GTATGTCCGA CCTGGGGGTG GGTACCAGCC CACCTTTAGT 
CTTACCCAAA AGTGTGACGT CAATGGGCAG AACGAGCATC CTGTCTTTGC CTACCTGAAA 
GACAAGCTGC CCTACCCTTA TGATGACCCG TTCTCCCTCA TGACCGATCC CAAGCTCATC 
ATATGGAGTC CCGTGCGCCG CTCAGACGTG TCCTGGAACT TTGAGAAGTT CCTCATAGGG 
CCAGAAGGGG AGCCCTTCCG TCGCTACAGC CGCAGCTTCC AGACCATCAA CATCGAGCCT 
GACATCAAAC GGCTCCTCAA AGTTGCCATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
PLoS genetics12(9)e1006355(2016 Sep)
Wu Y,Wei FY,Kawarada L,Suzuki T,Araki K,Komohara Y,Fujimura A,Kaitsuka T,Takeya M,Oike Y,Suzuki T,Tomizawa K

Cold/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock-induced oxidation.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(9)3155-70(2016 09)
Borowiec AS,Sion B,Chalmel F,D Rolland A,Lemonnier L,De Clerck T,Bokhobza A,Derouiche S,Dewailly E,Slomianny C,Mauduit C,Benahmed M,Roudbaraki M,J?gou B,Prevarskaya N,Bidaux G

Deletion of glutathione peroxidase-2 inhibits azoxymethane-induced colon cancer development.
PloS one8(8)e72055(2013)
M?ller MF,Florian S,Pommer S,Osterhoff M,Esworthy RS,Chu FF,Brigelius-Floh? R,Kipp AP

Expression of extracellular glutathione peroxidase in human and mouse gastrointestinal tract.
The American journal of physiology275(6 Pt 1)G1463-71(1998 Dec)
Tham DM,Whitin JC,Kim KK,Zhu SX,Cohen HJ

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Gpx2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報