Dio1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dio1
Entrez Gene ID	25430
Full Name	iodothyronine deiodinase 1
Synonyms	5DI,ITDI1,TXDI1
General protein information	Preferred Names iodothyronine deiodinase 1 Names type I iodothyronine deiodinase DIOI deiodinase, iodothyronine, type I thyroxine deiodinase, type I type 1 DI type-I 5'-deiodinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q34
Summary	The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the activation, as well as the inactivation of thyroid hormone by outer and inner ring deiodination, respectively. The activation reaction involves the conversion of the prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4), secreted by the thyroid gland, to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by 5'-deiodination. This protein is expressed predominantly in the liver and kidney and provides most of the circulating T3, which is essential for growth, differentiation and basal metabolism in vertebrates. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021653.4	NP_067685.5	type I iodothyronine deiodinase isoform 1
NM_001324210.1	NP_001311139.1	type I iodothyronine deiodinase isoform 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DIO1	NP_001116065.1
Rattus norvegicus (Norway rat)	Dio1	NP_067685.5
Gallus gallus (chicken)	DIO1	NP_001091083.1
Danio rerio (zebrafish)	dio1	NP_001007284.2
Xenopus tropicalis (tropical clawed frog)	dio1	NP_001243226.1
Homo sapiens (human)	DIO1	NP_000783.2
Canis lupus familiaris (dog)	DIO1	NP_001007127.1
	DIO1	NP_001116124.1
Mus musculus (house mouse)	Dio1	NP_031886.3
Pan troglodytes (chimpanzee)	DIO1	NP_001116123.1

Gene Ontology
Bibliography

Related articles in PubMed

Thyroid hormone and estrogen regulate exercise-induced growth hormone release.
Ignacio DL, da S Silvestre DH, Cavalcanti-de-Albuquerque JP, Louzada RA, Carvalho DP, Werneck-de-Castro JP
PloS one10(4)e0122556(2015)

Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity.
Sosic-Jurjevic B, Filipovic B, Renko K, Ajdzanovic V, Manojlovic-Stojanoski M, Milosevic V, K?hrle J
The Journal of endocrinology215(2)247-56(2012 Nov)

Blunted response of pituitary type 1 and brown adipose tissue type 2 deiodinases to swimming training in ovariectomized rats.
Ignacio DL, Fortunato RS, Neto RA, da Silva Silvestre DH, Nigro M, Frankenfeld TG, Werneck-de-Castro JP, Carvalho DP
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme44(11)797-803(2012 Oct)

Presence and regulation of D1 and D2 deiodinases in rat white adipose tissue.
Calvo RM, Obregon MJ
Metabolism: clinical and experimental60(9)1207-10(2011 Sep)

Regulatory role of the 3' untranslated region (3'UTR) of rat 5' deiodinase (D1). effects on messenger RNA translation and stability.
Arroyo-Helguera O, Mej?a-Viggiano C, Varela-Echavarr?a A, Cajero-Ju?rez M, Aceves C
Endocrine27(3)219-25(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

estrogen deficiency impairs the induction of thyroid hormone activating enzyme D1 in the pituitary, and GH release by acute exercise. Also, acute D1 activation is essential for exercise-induced GH response
Title: Thyroid hormone and estrogen regulate exercise-induced growth hormone release.

The effects of androgen deprivation on thyroid homeostasis, reflected in DioI and DioII expression, are reported.
Title: Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity.

Response of pituitary Dio1 and brown adipose tissue Dio2 is blunted in ovariectomized rats.
Title: Blunted response of pituitary type 1 and brown adipose tissue type 2 deiodinases to swimming training in ovariectomized rats.

Type 1 and type 2 deiodinase may have distinct functions in white adipose tissue, regulating lipogenesis and lipolysis.
Title: Presence and regulation of D1 and D2 deiodinases in rat white adipose tissue.

The study provided first evidence on the reduction of liver RAR concentrations and 5'-DI activity in response to long-term voluntary wheel running.
Title: Changes in retinoic acid receptor status, 5'-deiodinase activity and neuroendocrine response to voluntary wheel running.

The following Dio1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dio1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12115	NM_021653.4 Latest version!	Rattus norvegicus iodothyronine deiodinase 1 (Dio1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa63245	NM_001324210.1 Latest version!	Rattus norvegicus iodothyronine deiodinase 1 (Dio1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dio1 ( NM_021653.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_067685.5 Rattus norvegicus iodothyronine deiodinase 1 (Dio1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12115
Clone ID Related Accession (Same CDS sequence)	NM_021653.4
Accession Version	NM_021653.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Rattus norvegicus(Norway rat)
Product	type I iodothyronine deiodinase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC114866.4 and BC083557.1. On Apr 27, 2016 this sequence version replaced NM_021653.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X57999.1, BC083557.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906355 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 1825132 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTGT CCCAGCTATG GCTGTGGCTG AAGCGGCTTG TGATATTCCT GCAGGTAGCC 
TTGGAGGTGG CTACGGGCAA GGTGCTAATG ACACTGTTCC CAGAGAGAGT CAAGCAGAAC 
ATCCTGGCCA TGGGCCAAAA GACCGGAATG ACCAGGAATC CCCGATTCGC CCCTGACAAC 
TGGGTCCCCA CCTTCTTCAG CATCCAGTAC TTCTGGTTCG TCCTGAAGGT CCGCTGGCAG 
AGACTGGAAG ACAGGGCTGA GTATGGGGGG CTGGCCCCCA ACTGCACCGT GGTCCGCCTC 
TCAGGACAGA AGTGCAACGT CTGGGATTTC ATTCAAGGCA GCAGACCCCT GGTGTTGAAC 
TTCGGCAGCT GCACCTGACC TTCATTTCTT CTCAAATTTG ACCAGTTCAA GAGACTCGTA 
GACGACTTTG CCTCCACAGC TGACTTCCTC ATCATTTACA TTGAAGAAGC TCACGCCACA 
GATGGATGGG CTTTTAAGAA CAACGTGGAC ATCAGGCAGC ACCGAAGCCT CCAGGACCGC 
CTGCGGGCAG CACATCTGCT GCTGGCCAGG AGCCCCCAGT GTCCTGTGGT GGTGGACACA 
ATGCAGAACC AGAGCAGCCA GCTCTATGCA GCTCTGCCTG AGAGGCTCTA TGTGATACAG 
GAAGGCAGGA TCTGCTACAA GGGTAAACCT GGCCCTTGGA ACTACAATCC TGAGGAAGTC 
CGAGCTGTTC TGGAAAAGCT TTGCATCCCA CCTGGACACA TGCCTCAGTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067685.5
CDS	20..793
Translation	MGLSQLWLWLKRLVIFLQVALEVATGKVLMTLFPERVKQNILAMGQKTGMTRNPRFAPDNWVPTFFSIQYFWFVLKVRWQRLEDRAEYGGLAPNCTVVRLSGQKCNVWDFIQGSRPLVLNFGSCT*PSFLLKFDQFKRLVDDFASTADFLIIYIEEAHATDGWAFKNNVDIRQHRSLQDRLRAAHLLLARSPQCPVVVDTMQNQSSQLYAALPERLYVIQEGRICYKGKPGPWNYNPEEVRAVLEKLCIPPGHMPQF

Target ORF information:

RefSeq Version	NM_021653.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus iodothyronine deiodinase 1 (Dio1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021653.4

ORF Insert Sequence:

ATGGGGCTGT CCCAGCTATG GCTGTGGCTG AAGCGGCTTG TGATATTCCT GCAGGTAGCC 
TTGGAGGTGG CTACGGGCAA GGTGCTAATG ACACTGTTCC CAGAGAGAGT CAAGCAGAAC 
ATCCTGGCCA TGGGCCAAAA GACCGGAATG ACCAGGAATC CCCGATTCGC CCCTGACAAC 
TGGGTCCCCA CCTTCTTCAG CATCCAGTAC TTCTGGTTCG TCCTGAAGGT CCGCTGGCAG 
AGACTGGAAG ACAGGGCTGA GTATGGGGGG CTGGCCCCCA ACTGCACCGT GGTCCGCCTC 
TCAGGACAGA AGTGCAACGT CTGGGATTTC ATTCAAGGCA GCAGACCCCT GGTGTTGAAC 
TTCGGCAGCT GCACCTGACC TTCATTTCTT CTCAAATTTG ACCAGTTCAA GAGACTCGTA 
GACGACTTTG CCTCCACAGC TGACTTCCTC ATCATTTACA TTGAAGAAGC TCACGCCACA 
GATGGATGGG CTTTTAAGAA CAACGTGGAC ATCAGGCAGC ACCGAAGCCT CCAGGACCGC 
CTGCGGGCAG CACATCTGCT GCTGGCCAGG AGCCCCCAGT GTCCTGTGGT GGTGGACACA 
ATGCAGAACC AGAGCAGCCA GCTCTATGCA GCTCTGCCTG AGAGGCTCTA TGTGATACAG 
GAAGGCAGGA TCTGCTACAA GGGTAAACCT GGCCCTTGGA ACTACAATCC TGAGGAAGTC 
CGAGCTGTTC TGGAAAAGCT TTGCATCCCA CCTGGACACA TGCCTCAGTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dio1 ( NM_001324210.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001311139.1 Rattus norvegicus iodothyronine deiodinase 1 (Dio1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa63245
Clone ID Related Accession (Same CDS sequence)	NM_001324210.1
Accession Version	NM_001324210.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Rattus norvegicus(Norway rat)
Product	type I iodothyronine deiodinase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC114866.4 and BC083557.1. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which causes a frameshift compared to variant 1. The resulting isoform (2) is shorter, with a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CK473178.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 1825132 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTGT CCCAGCTATG GCTGTGGCTG AAGCGGCTTG TGATATTCCT GCAGGTAGCC 
TTGGAGGTGG CTACGGGCAA GGTGCTAATG ACACTGTTCC CAGAGAGAGT CAAGCAGAAC 
ATCCTGGCCA TGGGCCAAAA GACCGGAATG ACCAGGAATC CCCGATTCGC CCCTGACAAC 
TGGGTCCCCA CCTTCTTCAG CATCCAGTAC TTCTGGTTCG TCCTGAAGGT CCGCTGGCAG 
AGACTGGAAG ACAGGGCTGA GTATGGGGGG CTGGCCCCCA ACTGCACCGT GGTCCGCCTC 
TCAGGACAGA AGTGCAACGT CTGGGATTTC ATTCAAGGCA GCAGACCCCT GGTGTTGAAC 
TTCGGCAGCT GCACCTGACC TTCATTTCTT CTCAAATTTG ACCAGTTCAA GAGACTCGTA 
GACGACTTTG CCTCCACAGC TGACTTCCTC ATCATTTACA TTGAAGAAGC TCACGCCACA 
GGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311139.1
CDS	20..505
Translation	MGLSQLWLWLKRLVIFLQVALEVATGKVLMTLFPERVKQNILAMGQKTGMTRNPRFAPDNWVPTFFSIQYFWFVLKVRWQRLEDRAEYGGLAPNCTVVRLSGQKCNVWDFIQGSRPLVLNFGSCT*PSFLLKFDQFKRLVDDFASTADFLIIYIEEAHATG

Target ORF information:

RefSeq Version	NM_001324210.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus iodothyronine deiodinase 1 (Dio1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324210.1

ORF Insert Sequence:

ATGGGGCTGT CCCAGCTATG GCTGTGGCTG AAGCGGCTTG TGATATTCCT GCAGGTAGCC 
TTGGAGGTGG CTACGGGCAA GGTGCTAATG ACACTGTTCC CAGAGAGAGT CAAGCAGAAC 
ATCCTGGCCA TGGGCCAAAA GACCGGAATG ACCAGGAATC CCCGATTCGC CCCTGACAAC 
TGGGTCCCCA CCTTCTTCAG CATCCAGTAC TTCTGGTTCG TCCTGAAGGT CCGCTGGCAG 
AGACTGGAAG ACAGGGCTGA GTATGGGGGG CTGGCCCCCA ACTGCACCGT GGTCCGCCTC 
TCAGGACAGA AGTGCAACGT CTGGGATTTC ATTCAAGGCA GCAGACCCCT GGTGTTGAAC 
TTCGGCAGCT GCACCTGACC TTCATTTCTT CTCAAATTTG ACCAGTTCAA GAGACTCGTA 
GACGACTTTG CCTCCACAGC TGACTTCCTC ATCATTTACA TTGAAGAAGC TCACGCCACA 
GGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Thyroid hormone and estrogen regulate exercise-induced growth hormone release.
PloS one10(4)e0122556(2015)
Ignacio DL,da S Silvestre DH,Cavalcanti-de-Albuquerque JP,Louzada RA,Carvalho DP,Werneck-de-Castro JP

Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity.
The Journal of endocrinology215(2)247-56(2012 Nov)
Sosic-Jurjevic B,Filipovic B,Renko K,Ajdzanovic V,Manojlovic-Stojanoski M,Milosevic V,K?hrle J

Blunted response of pituitary type 1 and brown adipose tissue type 2 deiodinases to swimming training in ovariectomized rats.
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme44(11)797-803(2012 Oct)
Ignacio DL,Fortunato RS,Neto RA,da Silva Silvestre DH,Nigro M,Frankenfeld TG,Werneck-de-Castro JP,Carvalho DP

Presence and regulation of D1 and D2 deiodinases in rat white adipose tissue.
Metabolism: clinical and experimental60(9)1207-10(2011 Sep)
Calvo RM,Obregon MJ

Regulatory role of the 3' untranslated region (3'UTR) of rat 5' deiodinase (D1). effects on messenger RNA translation and stability.
Endocrine27(3)219-25(2005 Aug)
Arroyo-Helguera O,Mej?a-Viggiano C,Varela-Echavarr?a A,Cajero-Ju?rez M,Aceves C

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Dio1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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