ORF » Species Summary » Danio rerio » dio1 cDNA ORF clone

dio1 cDNA ORF clone, Danio rerio(zebrafish)

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  • Clones

  • gRNAs

The following dio1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dio1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa98437 NM_001007283.2
Latest version!		 Danio rerio iodothyronine deiodinase 1 (dio1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
ODa98438 NM_001324404.1
Latest version!		 Danio rerio iodothyronine deiodinase 1 (dio1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    dio1 ( NM_001007283.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007284.3 Danio rerio iodothyronine deiodinase 1 (dio1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID ODa98437
    Clone ID Related Accession (Same CDS sequence) NM_001007283.2
    Accession Version NM_001007283.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Danio rerio(zebrafish)
    Product type I iodothyronine deiodinase isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CO929563.1, DN772220.1, CK864452.1, EH492365.1, EE331695.1, CD606655.1, CR847832.18 and BC155742.1. On May 2, 2016 this sequence version replaced NM_001007283.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC155742.1, GFIL01008647.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505382, SAMN02259099 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 12915322 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGGGTCCG CAGTGGGATT TGCGCTGAGA AAACTTTTTG TTTATATATC AGCCGTGTTG 
    ATGGTGTGCG CTGCGATCCT GCGGATGTCC ATGTTGAAGC TCCTGTCATT CATTTCCCCT 
    GGACGCATGA GGAAAATCCA TATGAAAATG GGGGAGAGGA CCACGATGAC CCAAAACCCC 
    AAATTCAGAT ACGAAGACTG GGGACCGGCT TTCTTCTCTC TGGCGTTCAT TAAAACCTTG 
    TTCTTCGTCA ACTGGTGCTC GCTGGGCCTC GAGGCGTTTG AGGGACACTC CGCTCCGGAC 
    AGCGCTTTAA TTACCCTGGA CCGACAAAAG ACGAGCGTTC ATCGTTTTCT TAAAGGTAAT 
    CGTCCCCTGG TTCTGAGTTT CGGAAGCTGC ACCTGACCCC CGTTTCTCTA CAAACTTGAC 
    GAGTTCAAAC AGCTTGTCAA GGACTTCAGC AATGTCGCAG ATTTCCTCAT TGTTTACCTT 
    GCTGAAGCTC ATGCAACTGA TGCATGGGCC TTTAAAAACA ATGTGGATAT CAGCGTGCAC 
    AAAAACTTGG AGGAGAGGCT TGCTGCGGCT CGGACCCTGC TCAAAGAAGA CCCTCCATGC 
    CCTGTGGTGG TGGATGAGAT GAACAACATT ACTGCCAGCA AATACGGAGC CCTGCCCGAG 
    CGGCTCTATG TCATTCAGAG TGGCAAAGTT ATCTATCAGG GAGGCATCGG ACCTTGGGGG 
    TACAAGCCTG AAGAGGTTCG AAAAGTTCTG GAAAAAAGTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001007284.3
    CDS139..903
    Translation

    Target ORF information:

    RefSeq Version NM_001007283.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio iodothyronine deiodinase 1 (dio1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001007283.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGGGTCCG CAGTGGGATT TGCGCTGAGA AAACTTTTTG TTTATATATC AGCCGTGTTG 
    ATGGTGTGCG CTGCGATCCT GCGGATGTCC ATGTTGAAGC TCCTGTCATT CATTTCCCCT 
    GGACGCATGA GGAAAATCCA TATGAAAATG GGGGAGAGGA CCACGATGAC CCAAAACCCC 
    AAATTCAGAT ACGAAGACTG GGGACCGGCT TTCTTCTCTC TGGCGTTCAT TAAAACCTTG 
    TTCTTCGTCA ACTGGTGCTC GCTGGGCCTC GAGGCGTTTG AGGGACACTC CGCTCCGGAC 
    AGCGCTTTAA TTACCCTGGA CCGACAAAAG ACGAGCGTTC ATCGTTTTCT TAAAGGTAAT 
    CGTCCCCTGG TTCTGAGTTT CGGAAGCTGC ACCTGACCCC CGTTTCTCTA CAAACTTGAC 
    GAGTTCAAAC AGCTTGTCAA GGACTTCAGC AATGTCGCAG ATTTCCTCAT TGTTTACCTT 
    GCTGAAGCTC ATGCAACTGA TGCATGGGCC TTTAAAAACA ATGTGGATAT CAGCGTGCAC 
    AAAAACTTGG AGGAGAGGCT TGCTGCGGCT CGGACCCTGC TCAAAGAAGA CCCTCCATGC 
    CCTGTGGTGG TGGATGAGAT GAACAACATT ACTGCCAGCA AATACGGAGC CCTGCCCGAG 
    CGGCTCTATG TCATTCAGAG TGGCAAAGTT ATCTATCAGG GAGGCATCGG ACCTTGGGGG 
    TACAAGCCTG AAGAGGTTCG AAAAGTTCTG GAAAAAAGTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    dio1 ( NM_001324404.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001311333.1 Danio rerio iodothyronine deiodinase 1 (dio1), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID ODa98438
    Clone ID Related Accession (Same CDS sequence) NM_001324404.1
    Accession Version NM_001324404.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Danio rerio(zebrafish)
    Product type I iodothyronine deiodinase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CO929563.1, DN772220.1, AY221259.1, EH492365.1, EE331695.1, CD606655.1, CR847832.18 and BC155742.1. Transcript Variant: This variant (2) uses an alternate acceptor splice site at the 3' terminal exon, which causes a frameshift compared to variant 1. The resulting isoform (2) is longer, with a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY221259.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 12915322 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGGGTCCG CAGTGGGATT TGCGCTGAGA AAACTTTTTG TTTATATATC AGCCGTGTTG 
    ATGGTGTGCG CTGCGATCCT GCGGATGTCC ATGTTGAAGC TCCTGTCATT CATTTCCCCT 
    GGACGCATGA GGAAAATCCA TATGAAAATG GGGGAGAGGA CCACGATGAC CCAAAACCCC 
    AAATTCAGAT ACGAAGACTG GGGACCGGCT TTCTTCTCTC TGGCGTTCAT TAAAACCTTG 
    TTCTTCGTCA ACTGGTGCTC GCTGGGCCTC GAGGCGTTTG AGGGACACTC CGCTCCGGAC 
    AGCGCTTTAA TTACCCTGGA CCGACAAAAG ACGAGCGTTC ATCGTTTTCT TAAAGGTAAT 
    CGTCCCCTGG TTCTGAGTTT CGGAAGCTGC ACCTGACCCC CGTTTCTCTA CAAACTTGAC 
    GAGTTCAAAC AGCTTGTCAA GGACTTCAGC AATGTCGCAG ATTTCCTCAT TGTTTACCTT 
    GCTGAAGCTC ATGCAACTGA TGCATGGGCC TTTAAAAACA ATGTGGATAT CAGCGTGCAC 
    AAAAACTTGG AGGAGAGGCT TGCTGCGGCT CGGACCCTGC TCAAAGAAGA CCCTCCATGC 
    CCTGTGGTGG TGGATGAGAT GAACAACATT ACTGCCAGCA AATACGGAGC CCTGCCCGAG 
    CGGCTCTATG TCATTCAGAG TGGCAAAGTT ATCTATCAGG CATCGGACCT TGGGGGTACA 
    AGCCTGAAGA GGTTCGAAAA GTTCTGGAAA AAAGTAAATA AATGGAGAAG AATGGCAAAA 
    TGTTTTTTAT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311333.1
    CDS139..933
    Translation

    Target ORF information:

    RefSeq Version NM_001324404.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio iodothyronine deiodinase 1 (dio1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001324404.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGGGTCCG CAGTGGGATT TGCGCTGAGA AAACTTTTTG TTTATATATC AGCCGTGTTG 
    ATGGTGTGCG CTGCGATCCT GCGGATGTCC ATGTTGAAGC TCCTGTCATT CATTTCCCCT 
    GGACGCATGA GGAAAATCCA TATGAAAATG GGGGAGAGGA CCACGATGAC CCAAAACCCC 
    AAATTCAGAT ACGAAGACTG GGGACCGGCT TTCTTCTCTC TGGCGTTCAT TAAAACCTTG 
    TTCTTCGTCA ACTGGTGCTC GCTGGGCCTC GAGGCGTTTG AGGGACACTC CGCTCCGGAC 
    AGCGCTTTAA TTACCCTGGA CCGACAAAAG ACGAGCGTTC ATCGTTTTCT TAAAGGTAAT 
    CGTCCCCTGG TTCTGAGTTT CGGAAGCTGC ACCTGACCCC CGTTTCTCTA CAAACTTGAC 
    GAGTTCAAAC AGCTTGTCAA GGACTTCAGC AATGTCGCAG ATTTCCTCAT TGTTTACCTT 
    GCTGAAGCTC ATGCAACTGA TGCATGGGCC TTTAAAAACA ATGTGGATAT CAGCGTGCAC 
    AAAAACTTGG AGGAGAGGCT TGCTGCGGCT CGGACCCTGC TCAAAGAAGA CCCTCCATGC 
    CCTGTGGTGG TGGATGAGAT GAACAACATT ACTGCCAGCA AATACGGAGC CCTGCCCGAG 
    CGGCTCTATG TCATTCAGAG TGGCAAAGTT ATCTATCAGG CATCGGACCT TGGGGGTACA 
    AGCCTGAAGA GGTTCGAAAA GTTCTGGAAA AAAGTAAATA AATGGAGAAG AATGGCAAAA 
    TGTTTTTTAT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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