Dio1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dio1
Entrez Gene ID	13370
Full Name	deiodinase, iodothyronine, type I
Synonyms	5DI,D1,ITDI1,TXDI1
General protein information	Preferred Names deiodinase, iodothyronine, type I Names type I iodothyronine deiodinase DIOI type 1 DI type-I 5'-deiodinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 C7\|4 50.18 cM
Summary	The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the activation, as well as the inactivation of thyroid hormone by outer and inner ring deiodination, respectively. The activation reaction involves the conversion of the prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4), secreted by the thyroid gland, to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by 5'-deiodination. This protein is expressed predominantly in the liver and kidney and provides most of the circulating T3, which is essential for growth, differentiation and basal metabolism in vertebrates. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Apr 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007860.3	NP_031886.3	type I iodothyronine deiodinase
NM_007860.4	NP_031886.3	type I iodothyronine deiodinase

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DIO1	NP_001116065.1
Rattus norvegicus (Norway rat)	Dio1	NP_067685.5
Gallus gallus (chicken)	DIO1	NP_001091083.1
Danio rerio (zebrafish)	dio1	NP_001007284.2
Xenopus tropicalis (tropical clawed frog)	dio1	NP_001243226.1
Homo sapiens (human)	DIO1	NP_000783.2
Canis lupus familiaris (dog)	DIO1	NP_001007127.1
	DIO1	NP_001116124.1
Mus musculus (house mouse)	Dio1	NP_031886.3
Pan troglodytes (chimpanzee)	DIO1	NP_001116123.1

Gene Ontology
Bibliography

Related articles in PubMed

An Essential Physiological Role for MCT8 in Bone in Male Mice.
Leitch VD, Di Cosmo C, Liao XH, O'Boy S, Galliford TM, Evans H, Croucher PI, Boyde A, Dumitrescu A, Weiss RE, Refetoff S, Williams GR, Bassett JHD
Endocrinology158(9)3055-3066(2017 Sep)

Citterio CE, Veluswamy B, Morgan SJ, Galton VA, Banga JP, Atkins S, Morishita Y, Neumann S, Latif R, Gershengorn MC, Smith TJ, Arvan P
The Journal of biological chemistry292(37)15434-15444(2017 09)

Type 1 Deiodinase Regulates ApoA-I Gene Expression and ApoA-I Synthesis Independent of Thyroid Hormone Signaling.
Liu J, Hernandez-Ono A, Graham MJ, Galton VA, Ginsberg HN
Arteriosclerosis, thrombosis, and vascular biology36(7)1356-66(2016 Jul)

Perinatal deiodinase 2 expression in hepatocytes defines epigenetic susceptibility to liver steatosis and obesity.
Fonseca TL, Fernandes GW, McAninch EA, Bocco BM, Abdalla SM, Ribeiro MO, Moh?csik P, Fekete C, Li D, Xing X, Wang T, Gereben B, Bianco AC
Proceedings of the National Academy of Sciences of the United States of America112(45)14018-23(2015 Nov)

Characterization of mammalian selenoproteomes.
Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guig? R, Gladyshev VN
Science (New York, N.Y.)300(5624)1439-43(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Reductions in Dio1 expression reduce the expression of ApoA-I in a 3,5,3'-triiodothyronine-/thyroid hormone response element-independent manner.
Title: Type 1 Deiodinase Regulates ApoA-I Gene Expression and ApoA-I Synthesis Independent of Thyroid Hormone Signaling.

Within the supportive environment of a research vivarium, mice lacking all three deiodinases can be bred and survive.
Title: Life without the iodothyronine deiodinases.

Systemic changes in thyroid hormone economy as a result of acute food deprivation are not dependent on the deiodinases D1 or D2 but are mediated in part by sequestration of type4 and type3 in tissues and their enhanced metabolism by deiodinase D3.
Title: The 5'-deiodinases are not essential for the fasting-induced decrease in circulating thyroid hormone levels in male mice: possible roles for the type 3 deiodinase and tissue sequestration of hormone.

Results demonstrate changes in D1 activity in white adipose tissue under conditions of changing adiposity, and a stimulatory effect of leptin on D1 activity in the tissue.
Title: Modulation of type I iodothyronine 5'-deiodinase activity in white adipose tissue by nutrition: possible involvement of leptin.

D1 playing an important role in iodide conservation by serving as a scavenger enzyme in peripheral tissues and the thyroid.
Title: Life without thyroxine to 3,5,3'-triiodothyronine conversion: studies in mice devoid of the 5'-deiodinases.

The following Dio1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dio1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15022	NM_007860.4 Latest version!	Mus musculus deiodinase, iodothyronine, type I (Dio1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
View Old Accession Versions >>
OMu15022	NM_007860.3	Mus musculus deiodinase, iodothyronine, type I (Dio1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dio1 ( NM_007860.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031886.3 Mus musculus deiodinase, iodothyronine, type I (Dio1), mRNA.

Price & Availability↑

CloneID	OMu15022
Clone ID Related Accession (Same CDS sequence)	NM_007860.3 , NM_007860.4
Accession Version	NM_007860.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-09
Organism	Mus musculus(House mouse)
Product	type I iodothyronine deiodinase
Comment

ATGGGGCTGC CCCAGCTATG GCTGTGGCTG AAGCGGCTTG TGATATTCCT GCAGGTGGCC 
TTGGAGGTGG CTGTAGGCAA GGTGCTAATG ACGCTGTTCC CAGGGAGAGT CAAACAGAGC 
ATCCTGGCCA TGGGCCAGAA GACCGGGATG GCCAGGAACC CCCGATTCGC CCCTGACAAC 
TGGGTCCCCA CCTTCTTCAG CATCCAGTAC TTCTGGTTTG TCCTGAAGGT CCGCTGGCAG 
AGACTGGAAG ACAGGGCTGA GTTTGGGGGG CTGGCCCCCA ACTGCACCGT GGTCTGCCTC 
TCAGGACAGA AGTGCAACAT CTGGGATTTC ATTCAAGGCA GCAGGCCCCT GGTGTTGAAC 
TTTGGCAGTT GCACCTGACC TTCATTTCTT CTCAAATTTG ACCAGTTCAA GAGACTCGTA 
GATGACTTTG CCTCCACAGC CGATTTCCTC ATCATTTACA TTGAAGAAGC TCACGCCACA 
GATGGCTGGG CTTTTAAGAA CAACGTGGAC ATCCGGCAGC ACCGGAGCCT CCAGGAGCGC 
GTGCGGGCAG CCCGCATGCT GCTGGCCAGG AGCCCCCAGT GCCCTGTGGT GGTGGACACA 
ATGCAGAACC AGAGCAGCCA GCTCTACGCG GCCCTGCCTG AGAGGCTCTA CGTGATACAG 
GAGGGCAGGA TCTGCTACAA GGGTAAAGCT GGCCCTTGGA ACTACAATCC TGAGGAAGTC 
CGAGCTGTCC TGGAAAAGCT TTGCACTCCA CCTAGACACG TGCCTCAGCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031886.3
Translation	MGLPQLWLWLKRLVIFLQVALEVAVGKVLMTLFPGRVKQSILAMGQKTGMARNPRFAPDNWVPTFFSIQYFWFVLKVRWQRLEDRAEFGGLAPNCTVVCLSGQKCNIWDFIQGSRPLVLNFGSCT*PSFLLKFDQFKRLVDDFASTADFLIIYIEEAHATDGWAFKNNVDIRQHRSLQERVRAARMLLARSPQCPVVVDTMQNQSSQLYAALPERLYVIQEGRICYKGKAGPWNYNPEEVRAVLEKLCTPPRHVPQL

Target ORF information:

RefSeq Version	NM_007860.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus deiodinase, iodothyronine, type I (Dio1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007860.3

ORF Insert Sequence:

ATGGGGCTGC CCCAGCTATG GCTGTGGCTG AAGCGGCTTG TGATATTCCT GCAGGTGGCC 
TTGGAGGTGG CTGTAGGCAA GGTGCTAATG ACGCTGTTCC CAGGGAGAGT CAAACAGAGC 
ATCCTGGCCA TGGGCCAGAA GACCGGGATG GCCAGGAACC CCCGATTCGC CCCTGACAAC 
TGGGTCCCCA CCTTCTTCAG CATCCAGTAC TTCTGGTTTG TCCTGAAGGT CCGCTGGCAG 
AGACTGGAAG ACAGGGCTGA GTTTGGGGGG CTGGCCCCCA ACTGCACCGT GGTCTGCCTC 
TCAGGACAGA AGTGCAACAT CTGGGATTTC ATTCAAGGCA GCAGGCCCCT GGTGTTGAAC 
TTTGGCAGTT GCACCTGACC TTCATTTCTT CTCAAATTTG ACCAGTTCAA GAGACTCGTA 
GATGACTTTG CCTCCACAGC CGATTTCCTC ATCATTTACA TTGAAGAAGC TCACGCCACA 
GATGGCTGGG CTTTTAAGAA CAACGTGGAC ATCCGGCAGC ACCGGAGCCT CCAGGAGCGC 
GTGCGGGCAG CCCGCATGCT GCTGGCCAGG AGCCCCCAGT GCCCTGTGGT GGTGGACACA 
ATGCAGAACC AGAGCAGCCA GCTCTACGCG GCCCTGCCTG AGAGGCTCTA CGTGATACAG 
GAGGGCAGGA TCTGCTACAA GGGTAAAGCT GGCCCTTGGA ACTACAATCC TGAGGAAGTC 
CGAGCTGTCC TGGAAAAGCT TTGCACTCCA CCTAGACACG TGCCTCAGCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dio1 ( NM_007860.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031886.3 Mus musculus deiodinase, iodothyronine, type I (Dio1), mRNA.

Price & Availability↑

CloneID	OMu15022
Clone ID Related Accession (Same CDS sequence)	NM_007860.3 , NM_007860.4
Accession Version	NM_007860.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	type I iodothyronine deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645723.11 and BC089608.1. On Apr 26, 2016 this sequence version replaced NM_007860.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC019786.1, U49861.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 8419134 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTGC CCCAGCTATG GCTGTGGCTG AAGCGGCTTG TGATATTCCT GCAGGTGGCC 
TTGGAGGTGG CTGTAGGCAA GGTGCTAATG ACGCTGTTCC CAGGGAGAGT CAAACAGAGC 
ATCCTGGCCA TGGGCCAGAA GACCGGGATG GCCAGGAACC CCCGATTCGC CCCTGACAAC 
TGGGTCCCCA CCTTCTTCAG CATCCAGTAC TTCTGGTTTG TCCTGAAGGT CCGCTGGCAG 
AGACTGGAAG ACAGGGCTGA GTTTGGGGGG CTGGCCCCCA ACTGCACCGT GGTCTGCCTC 
TCAGGACAGA AGTGCAACAT CTGGGATTTC ATTCAAGGCA GCAGGCCCCT GGTGTTGAAC 
TTTGGCAGTT GCACCTGACC TTCATTTCTT CTCAAATTTG ACCAGTTCAA GAGACTCGTA 
GATGACTTTG CCTCCACAGC CGATTTCCTC ATCATTTACA TTGAAGAAGC TCACGCCACA 
GATGGCTGGG CTTTTAAGAA CAACGTGGAC ATCCGGCAGC ACCGGAGCCT CCAGGAGCGC 
GTGCGGGCAG CCCGCATGCT GCTGGCCAGG AGCCCCCAGT GCCCTGTGGT GGTGGACACA 
ATGCAGAACC AGAGCAGCCA GCTCTACGCG GCCCTGCCTG AGAGGCTCTA CGTGATACAG 
GAGGGCAGGA TCTGCTACAA GGGTAAAGCT GGCCCTTGGA ACTACAATCC TGAGGAAGTC 
CGAGCTGTCC TGGAAAAGCT TTGCACTCCA CCTAGACACG TGCCTCAGCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031886.3
CDS	51..824
Translation	MGLPQLWLWLKRLVIFLQVALEVAVGKVLMTLFPGRVKQSILAMGQKTGMARNPRFAPDNWVPTFFSIQYFWFVLKVRWQRLEDRAEFGGLAPNCTVVCLSGQKCNIWDFIQGSRPLVLNFGSCT*PSFLLKFDQFKRLVDDFASTADFLIIYIEEAHATDGWAFKNNVDIRQHRSLQERVRAARMLLARSPQCPVVVDTMQNQSSQLYAALPERLYVIQEGRICYKGKAGPWNYNPEEVRAVLEKLCTPPRHVPQL

Target ORF information:

RefSeq Version	NM_007860.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus deiodinase, iodothyronine, type I (Dio1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007860.4

ORF Insert Sequence:

ATGGGGCTGC CCCAGCTATG GCTGTGGCTG AAGCGGCTTG TGATATTCCT GCAGGTGGCC 
TTGGAGGTGG CTGTAGGCAA GGTGCTAATG ACGCTGTTCC CAGGGAGAGT CAAACAGAGC 
ATCCTGGCCA TGGGCCAGAA GACCGGGATG GCCAGGAACC CCCGATTCGC CCCTGACAAC 
TGGGTCCCCA CCTTCTTCAG CATCCAGTAC TTCTGGTTTG TCCTGAAGGT CCGCTGGCAG 
AGACTGGAAG ACAGGGCTGA GTTTGGGGGG CTGGCCCCCA ACTGCACCGT GGTCTGCCTC 
TCAGGACAGA AGTGCAACAT CTGGGATTTC ATTCAAGGCA GCAGGCCCCT GGTGTTGAAC 
TTTGGCAGTT GCACCTGACC TTCATTTCTT CTCAAATTTG ACCAGTTCAA GAGACTCGTA 
GATGACTTTG CCTCCACAGC CGATTTCCTC ATCATTTACA TTGAAGAAGC TCACGCCACA 
GATGGCTGGG CTTTTAAGAA CAACGTGGAC ATCCGGCAGC ACCGGAGCCT CCAGGAGCGC 
GTGCGGGCAG CCCGCATGCT GCTGGCCAGG AGCCCCCAGT GCCCTGTGGT GGTGGACACA 
ATGCAGAACC AGAGCAGCCA GCTCTACGCG GCCCTGCCTG AGAGGCTCTA CGTGATACAG 
GAGGGCAGGA TCTGCTACAA GGGTAAAGCT GGCCCTTGGA ACTACAATCC TGAGGAAGTC 
CGAGCTGTCC TGGAAAAGCT TTGCACTCCA CCTAGACACG TGCCTCAGCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An Essential Physiological Role for MCT8 in Bone in Male Mice.
Endocrinology158(9)3055-3066(2017 Sep)
Leitch VD,Di Cosmo C,Liao XH,O'Boy S,Galliford TM,Evans H,Croucher PI,Boyde A,Dumitrescu A,Weiss RE,Refetoff S,Williams GR,Bassett JHD

The Journal of biological chemistry292(37)15434-15444(2017 09)
Citterio CE,Veluswamy B,Morgan SJ,Galton VA,Banga JP,Atkins S,Morishita Y,Neumann S,Latif R,Gershengorn MC,Smith TJ,Arvan P

Type 1 Deiodinase Regulates ApoA-I Gene Expression and ApoA-I Synthesis Independent of Thyroid Hormone Signaling.
Arteriosclerosis, thrombosis, and vascular biology36(7)1356-66(2016 Jul)
Liu J,Hernandez-Ono A,Graham MJ,Galton VA,Ginsberg HN

Perinatal deiodinase 2 expression in hepatocytes defines epigenetic susceptibility to liver steatosis and obesity.
Proceedings of the National Academy of Sciences of the United States of America112(45)14018-23(2015 Nov)
Fonseca TL,Fernandes GW,McAninch EA,Bocco BM,Abdalla SM,Ribeiro MO,Moh?csik P,Fekete C,Li D,Xing X,Wang T,Gereben B,Bianco AC

Characterization of mammalian selenoproteomes.
Science (New York, N.Y.)300(5624)1439-43(2003 May)
Kryukov GV,Castellano S,Novoselov SV,Lobanov AV,Zehtab O,Guig? R,Gladyshev VN

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Dio1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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