pyr-3 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	pyr-3
Entrez Gene ID	3877961
Full Name	pyrimidine-3
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_956720.2	XP_961813.1	pyrABCN

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAD	XP_001155357.1
Xenopus tropicalis (tropical clawed frog)	cad	XP_004920257.1
Canis lupus familiaris (dog)	CAD	XP_540124.3
Bos taurus (cattle)	CAD	NP_001178322.1
Gallus gallus (chicken)	CAD	XP_003641116.2
Kluyveromyces lactis	KLLA0E15357g	XP_454643.1
Eremothecium gossypii	AGOS_ACR263C	NP_983665.2
Neurospora crassa	NCU08287	XP_961813.1
Danio rerio (zebrafish)	cad	NP_001009884.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000300	XP_310823.5
Caenorhabditis elegans (roundworm)	pyr-1	NP_495838.2
Saccharomyces cerevisiae (baker's yeast)	URA2	NP_012405.2
Homo sapiens (human)	CAD	NP_004332.2
Rattus norvegicus (Norway rat)	Cad	NP_001099180.2
Drosophila melanogaster (fruit fly)	r	NP_523377.1
Schizosaccharomyces pombe (fission yeast)	ura1	NP_593055.1
	CAD	XP_002799221.1
Mus musculus (house mouse)	Cad	NP_076014.1
Magnaporthe oryzae (rice blast fungus)	MGG_01537	XP_003714510.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pyr-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pyr-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf03489	XM_956720.2 Latest version!	Neurospora crassa OR74A pyrABCN (pyr-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pyr-3 ( XM_956720.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_961813.1 Neurospora crassa OR74A pyrABCN (pyr-3), mRNA.

Price & Availability↑

CloneID	ONf03489
Clone ID Related Accession (Same CDS sequence)	XM_956720.2
Accession Version	XM_956720.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	pyrABCN
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_956720.1.

ATGGCTGCCA CCGTCTACCG CCCCGCCACG GCCCCCATCG CCGATGCGCC TTGCGAAGGC 
CTTGTTTGCC TGGAGCTTGA GGATGGCTCT GCCTACCAAG GCTACAGCTT TGGTGCGCCC 
AAGAGCATTG CCGGCGAGCT TGTCTTCCAG ACCGGCATGG TCGGCTACCC TGAGTCCGTG 
ACCGATCCTT CGTACCGCGG TCAGATTCTC GTCATTACCT TCCCTCTTGT TGGAAACTAT 
GGCGTCCCCT CGCGGGAGAC CGTCGATGAT CTGCTTAAGG ATCTCCCCGC CCACTTCGAG 
TCCAACCAGA TTCACATTGC CGGTCTCGTT ACCGCCTCTT ATGCTGGCGA AGACTTCTCG 
CACTTCCTCG CCACCTCGTC CCTTGGAACA TGGCTCAAGG AGCAGGGCAT CCCTGCCATG 
TACGGTGTCG ATACCCGCGC CCTCACCAAG CGCATCCGCG AGAAGGGTAG CATGCTTGGT 
CGCATGCTCC TCCAGAAGGA AGACTTGGCT GTCAGCGCTC CTTCTCTCGG AGTTCCTGGC 
GACTGGAAGC CTCACTTCGA GACTATCGAA TGGGTTAACC CCAACGAGAA GAACCTCGTT 
GCCGAAGTCT CGATCAAGGA GCCCAAGCTC TACAAGCCCG ATGAGGCCAC TGCCCTCAAG 
CACCCCTCGG GTCGCAACCT TCGTATTCTC TGCCTCGATG TCGGCATGAA GTACAACCAA 
CTCAGGTGCT TCATCAAGCG CGGTGTCGAA GTCCTCGTTT GCCCATGGGA CTACGATTTC 
TCAAAGGAAG AATATGATGG TCTGTTCATC TCCAACGGTC CTGGTGACCC GAAGGTCATG 
GACACCACTG TCGAGCACAT CTCTGCCGCC CTGCAGAAGA ACAACACACC CATCTTCGGT 
ATCTGCTTGG GTCACCAGCT GCTCGCTCGT GCTGCCGGTG CCAAGACGGT CAAGCTCAAG 
TTCGGTAACC GTGGCCACAA CATTCCTTGC ACCAGCATGG TCACCGGAAA GTGCCATATC 
ACCTCGCAGA ACCACGGTTA TGCTGTTGAC GCTACCACTC TCCCCACCGG ATGGCAGGAG 
CTGTTCATCA ACGCCAATGA TGGCAGCAAC GAGGGTATCA TGCACGTCGA CCGCCCCCAC 
TTCAGTGTCC AGTTCCACCC TGAGAGCACC CCCGGCCCCC GCGACACCGA GTTCCTCTTT 
GATGTCTTCA TTCAGACTGT TGCCAAGTGC ACTACCGATA ACACTCTCCT CCAGAAGGGT 
GTCGAATTCC CCGGTGGAAC CACTGAGGAG AATGAGAGAC TCCACCCCCG TGTCGATGTC 
AAGAAGGTCC TCGTCTTGGG CAGTGGTGGT CTCAGCATCG GTCAGGCTGG TGAATTCGAC 
TACTCGGGAT CTCAGGCCAT CAAGGCTTTG AAGGAAGAAG GTATCTACAC TGTCCTCATC 
AACCCCAACA TTGCCACCAT TCAGACCTCC AAGGGCTTGG CCGACAAGGT CTACTTCTTG 
CCAGTAAACG CCGAGTTCGT CCGGAAAGTT ATCAAGTACG AACAGCCCGA TGCCATCTAC 
TGCACCTTTG GTGGCCAAAC CGCTCTCTCG GTCGGTATCC AGCTCAAGGA TGAGTTTGAG 
GCTCTTGGTG TCAAGGTTCT CGGTACCCCC ATTGATACCA TCATCACCAC GGAGGACCGT 
GAGCTCTTTG CTCGCAGCAT GGAGTCCATT GGTGAGAAGT GTGCCAAGTC CGCTTCGGCC 
AGCAGTGTTG AGGAGGCCCT CAACGCGGTC AAGGACATTG GTTACCCGGT TATTGTTCGT 
GCAGCCTACG CTCTTGGTGG TCTCGGCAGT GGCTTCGCCA ACAACGAAGC TGAGCTCGTT 
GACCTGTGCA ACAAGGCGCT GGCAGCCAGT CCCCAGGTTC TTGTCGAGCG CAGTATGAAG 
GGCTGGAAGG AGATCGAGTA CGAGGTTGTT CGTGACGCCC AGGACAACTG TATCACTGTG 
TGCAACATGG AGAACTTCGA TCCTCTTGGC ATTCATACCG GCGATTCCAT TGTCGTCGCG 
CCTTCCCAGA CGCTTTCCGA TGAAGACTAC AACATGCTTC GCACTACTGC TGTCAACGTC 
ATTCGCCATC TTGGAGTTGT CGGCGAGTGT AACATCCAGT ATGCTCTGAA CCCCTTCTCC 
AGGGAGTACT GCATTATCGA AGTCAATGCC AGATTGTCGC GTTCCTCGGC CTTGGCTTCC 
AAGGCTACCG GATACCCCTT GGCTTTCATT GCTGCCAAGT TGGGTCTCGG TATTCCTCTG 
AAGGAGATTA AGAACACCGT TACCAAGGTG ACCTGCGCTT GCTTCGAGCC TTCTCTGGAT 
TATGTTGTGG TCAAGATGCC TCGTTGGGAT TTGAAGAAGT TCACCCGCGT CTCCACTCAG 
CTCGGCTCTT CCATGAAGAG TGTCGGCGAG GTCATGAGCA TCGGCAGGAC ATTTGAAGAG 
GCCATCCAGA AGGCTATTCG TGCCATCGAT TTCCACAACC TTGGCTTCAG CGAGTCCAAG 
GGTGCGCTCA TGTCCGTCGA CGACGAGCTC CAGACCCCCT CTGACCAGCG TCTATTCGCC 
ATTGCCAACG CCATGCACGC GGGCTACACT GTCGACAGAA TCTGGGAATT GACCAAGATC 
GACAAGTGGT TCTTGTCCAA GCTCAAGGGC CTGAGCAATT TCGCCAAGGA CATGACCAAA 
CTCACGGCTA ACGATGTTGT TGGTCGTCCT GATCTGCTCC TTCGCGCGAA GCAGCTTGGC 
TTCTGCGACC GTCAGCTTGC CAACTTCTGG GATTCCAACG AGCTAGCTAT TCGTCGCATG 
CGTCTTGAGG CAGGAATCAC TCCTTTCGTC AAGCAGATCG ACACCGTCGC CGCTGAATTC 
CCTGCCTTCA CCAACTACCT GTATCTGACC TACAACGCCA GCGAGCACGA TGTTTCGTTC 
GAAGACCGTG GTGTCATGGT TCTTGGTTCG GGTGTGTACC GCATTGGTTC TTCCGTCGAA 
TTTGATTGGT GTTCCGTTCG GGCCATTCGC ACGCTCCGCG AGTCTGGCTT CAAGACCATC 
ATGGTGAACT ACAACCCTGA GACCGTCAGT ACCGACTATG ATGAAGCCGA CAAGCTGTAC 
TTCGAGAACA TCAACCTGGA GACCGTTCTG GACGTCTATC AGATGGAGGA CGCAACCGGA 
GTGTTGGGAG CTATGGGTGG CCAAACCCCC AACAACATTG CTCTGCCCCT TCTTCGTGCT 
GGCGTCAGAG TTCTGGGTAC TTCTCCTGAG ATGATTGACA CTGCCGAGAA CCGCTACAAG 
TTCTCCCGCA TGCTTGACCG TATCGGTGTC GACCAGCCGA CTTGGAAGGA ACTTACCAGC 
TTCGATGAGG CGAAGGCCTT CTGCCAGAAG GTATCGTACC CGGTGCTGGT CAGACCTTCC 
TATGTGCTTT CTGGCGCTGC CATGAACACT GTCTACTCCG AGGCCGATCT TGAGTCCTAC 
CTTCAGCAGG CTGCCGATGT CTCTCCTGAG CACCCTGTCG TCATTACCAA GTACATCGAG 
AACGCCAAGG AAATCGAGAT GGACGCTGTG GCCAAGGATG GAAAGGTTGT TGGACATTTC 
ATTTCTGAAC ACGTTGAGAA CGCCGGTGTT CACTCTGGTG ATGCAACCCT TATCCTGCCG 
CCTCAGGATC TGGAGCAGAC CACTATCCAG CGCATCGAGG AAGCCACTCG CAAGATCGGT 
GCCGCTCTCA ACGTTACCGG CCCCTTCAAC ATCCAGTTCA TCGCCAAGGA CAACGACATC 
AAGGTGATTG AGTGCAACGT TCGCGCTTCG CGCTCTTTCC CCTTCGTCTC CAAGGTTATG 
GGAGTTGATC TGATCGAGAT GGCCACCAAG GCTATCATGG GTCTGCCTTT CGTAGAGTAC 
CCCGCCATCG ATCGTCCTTC TGGCGGTGTC GGTGTCAAGG TCCCCCAGTT CAGTTTCTCT 
CGTCTGTCTG GCGCTGACCC CGTCTTGGGT GTTGAGATGG CTTCCACTGG TGAAGTTGCC 
TCCTTTGGTG TTGACAAGTA CGAGGCCTAC CTGAAGGCTT TGATGTCCAC CGGCTTCAAG 
GTTCCCAAGA AGAACATTTT GCTCTCCATC GGTTCATACA AGGACAAGAA GGAGATGCTG 
CCTTCTGTGG CCAAGCTTTC TCAGATGGGC TACAAACTCT TCGCTACCGC TGGTACCGCC 
GATTTCCTTC AAGAGCACGA CATCCCCGTG CAATTCTTGG AGGTTCTCGC CAAGGAGGAC 
GATCAGAAGT CCGAGTACTC CCTCACCCAG CACTTGGCTA ACAACATGAT CGACCTGTAC 
ATCAACCTGC CCTCCAACAA CAGATACAGA CGCCCTGCCA ACTACATCAG CAAGGGTTAC 
CAGACACGTC GCATGGCTGT TGATTACCAG ATTCCCTTGG TCACCAACGT GAAGAACGCC 
AAGATCCTCG TCGAGGCTAT CGCCAGGTAC CGTGACATGG AGATCGGCGA GCGTGACTAC 
CAGACTAGCC ATACCCCACT TCAGCTCTCT GGGCAGGTCA ACTTCACCCT TCAGGACTCG 
CTCTCCAGGC CTCACTCTTT CAAGAAAGCC CACGTTCTCT CCGTAGAGCA GTACACTCGC 
GCCGACTTGC ATCTTCTTTT CACTGTTGCC GAGGAGATGC GCCTCAGTGT CCAGCGCGGA 
AACGTCATGG ACATTCTTAA GGGACGCATG CTTGCCACTC TCTTCTACGA GCCTTCCACC 
CGTACGTCGG CGTCTTTCGA GGCGGCTATG AAGCGTCTGG GCGGTGAAGT TATTTCCATC 
GCTACGCAAC ACTCCTCTGT TCAGAAGGGC GAGACTCTGC AGGACACGCT CCGTACTCTT 
GCCTGCTACG CCGACGCCAT TGTTCTCCGC CATCCCGACG AGACCTGCGT TGACGTTGCC 
AAGAAGTACA GCCCGGTGCC TGTTGTCAAC GCTGGTAACG GAAGCAGGGA GCACCCCACC 
CAGGCTTTCT TGGATCTCTT CACTGTCCGT GAGGAGCTGG GCACTGTCCA GGGCTTGACC 
TTCACGTTCG TCGGTGACCT TCGCTATGGC AGACCTGTGC ATTCCCTGGT CTACTTGCTT 
CGTCACTATT CGGGAGTCAA GGTGCAGCTC GTCTCCCCCA AGGGACTTGA GTTGCCTACC 
GATGTTCGTC AGCAACTGGT CAAGGCCGGC CAGCTGCTCT GCGAGTCTGA GACGTTGACC 
CCTGAGATTC TCGGCAAGAC TGATGTCTTG TACTGCACTC GTGTGCAAAA GGAGAGGTTC 
CCGAGCGAGG CCGAGTATGA GAAGGTGAAG GGTTCTTACC GCATCGACAA CCAGACCCTT 
AAGCACGCCA AGAGCAAGAT GGCTATCATG CACCCGCTCC CCAGAAACGA GGAGATTGCT 
GAGGAGGTTG ACTTCGACCA GCGGGCCGCA TACTTCAGAC AGATGAGATA TGGTCTCTAC 
TGCCGTATGG CCCTCCTGGC GTTGGTCATG TCTGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_961813.1
CDS	500..5998
Translation	MAATVYRPATAPIADAPCEGLVCLELEDGSAYQGYSFGAPKSIAGELVFQTGMVGYPESVTDPSYRGQILVITFPLVGNYGVPSRETVDDLLKDLPAHFESNQIHIAGLVTASYAGEDFSHFLATSSLGTWLKEQGIPAMYGVDTRALTKRIREKGSMLGRMLLQKEDLAVSAPSLGVPGDWKPHFETIEWVNPNEKNLVAEVSIKEPKLYKPDEATALKHPSGRNLRILCLDVGMKYNQLRCFIKRGVEVLVCPWDYDFSKEEYDGLFISNGPGDPKVMDTTVEHISAALQKNNTPIFGICLGHQLLARAAGAKTVKLKFGNRGHNIPCTSMVTGKCHITSQNHGYAVDATTLPTGWQELFINANDGSNEGIMHVDRPHFSVQFHPESTPGPRDTEFLFDVFIQTVAKCTTDNTLLQKGVEFPGGTTEENERLHPRVDVKKVLVLGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTVLINPNIATIQTSKGLADKVYFLPVNAEFVRKVIKYEQPDAIYCTFGGQTALSVGIQLKDEFEALGVKVLGTPIDTIITTEDRELFARSMESIGEKCAKSASASSVEEALNAVKDIGYPVIVRAAYALGGLGSGFANNEAELVDLCNKALAASPQVLVERSMKGWKEIEYEVVRDAQDNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPFSREYCIIEVNARLSRSSALASKATGYPLAFIAAKLGLGIPLKEIKNTVTKVTCACFEPSLDYVVVKMPRWDLKKFTRVSTQLGSSMKSVGEVMSIGRTFEEAIQKAIRAIDFHNLGFSESKGALMSVDDELQTPSDQRLFAIANAMHAGYTVDRIWELTKIDKWFLSKLKGLSNFAKDMTKLTANDVVGRPDLLLRAKQLGFCDRQLANFWDSNELAIRRMRLEAGITPFVKQIDTVAAEFPAFTNYLYLTYNASEHDVSFEDRGVMVLGSGVYRIGSSVEFDWCSVRAIRTLRESGFKTIMVNYNPETVSTDYDEADKLYFENINLETVLDVYQMEDATGVLGAMGGQTPNNIALPLLRAGVRVLGTSPEMIDTAENRYKFSRMLDRIGVDQPTWKELTSFDEAKAFCQKVSYPVLVRPSYVLSGAAMNTVYSEADLESYLQQAADVSPEHPVVITKYIENAKEIEMDAVAKDGKVVGHFISEHVENAGVHSGDATLILPPQDLEQTTIQRIEEATRKIGAALNVTGPFNIQFIAKDNDIKVIECNVRASRSFPFVSKVMGVDLIEMATKAIMGLPFVEYPAIDRPSGGVGVKVPQFSFSRLSGADPVLGVEMASTGEVASFGVDKYEAYLKALMSTGFKVPKKNILLSIGSYKDKKEMLPSVAKLSQMGYKLFATAGTADFLQEHDIPVQFLEVLAKEDDQKSEYSLTQHLANNMIDLYINLPSNNRYRRPANYISKGYQTRRMAVDYQIPLVTNVKNAKILVEAIARYRDMEIGERDYQTSHTPLQLSGQVNFTLQDSLSRPHSFKKAHVLSVEQYTRADLHLLFTVAEEMRLSVQRGNVMDILKGRMLATLFYEPSTRTSASFEAAMKRLGGEVISIATQHSSVQKGETLQDTLRTLACYADAIVLRHPDETCVDVAKKYSPVPVVNAGNGSREHPTQAFLDLFTVREELGTVQGLTFTFVGDLRYGRPVHSLVYLLRHYSGVKVQLVSPKGLELPTDVRQQLVKAGQLLCESETLTPEILGKTDVLYCTRVQKERFPSEAEYEKVKGSYRIDNQTLKHAKSKMAIMHPLPRNEEIAEEVDFDQRAAYFRQMRYGLYCRMALLALVMSG

Target ORF information:

RefSeq Version	XM_956720.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A pyrABCN (pyr-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_956720.2

ORF Insert Sequence:

ATGGCTGCCA CCGTCTACCG CCCCGCCACG GCCCCCATCG CCGATGCGCC TTGCGAAGGC 
CTTGTTTGCC TGGAGCTTGA GGATGGCTCT GCCTACCAAG GCTACAGCTT TGGTGCGCCC 
AAGAGCATTG CCGGCGAGCT TGTCTTCCAG ACCGGCATGG TCGGCTACCC TGAGTCCGTG 
ACCGATCCTT CGTACCGCGG TCAGATTCTC GTCATTACCT TCCCTCTTGT TGGAAACTAT 
GGCGTCCCCT CGCGGGAGAC CGTCGATGAT CTGCTTAAGG ATCTCCCCGC CCACTTCGAG 
TCCAACCAGA TTCACATTGC CGGTCTCGTT ACCGCCTCTT ATGCTGGCGA AGACTTCTCG 
CACTTCCTCG CCACCTCGTC CCTTGGAACA TGGCTCAAGG AGCAGGGCAT CCCTGCCATG 
TACGGTGTCG ATACCCGCGC CCTCACCAAG CGCATCCGCG AGAAGGGTAG CATGCTTGGT 
CGCATGCTCC TCCAGAAGGA AGACTTGGCT GTCAGCGCTC CTTCTCTCGG AGTTCCTGGC 
GACTGGAAGC CTCACTTCGA GACTATCGAA TGGGTTAACC CCAACGAGAA GAACCTCGTT 
GCCGAAGTCT CGATCAAGGA GCCCAAGCTC TACAAGCCCG ATGAGGCCAC TGCCCTCAAG 
CACCCCTCGG GTCGCAACCT TCGTATTCTC TGCCTCGATG TCGGCATGAA GTACAACCAA 
CTCAGGTGCT TCATCAAGCG CGGTGTCGAA GTCCTCGTTT GCCCATGGGA CTACGATTTC 
TCAAAGGAAG AATATGATGG TCTGTTCATC TCCAACGGTC CTGGTGACCC GAAGGTCATG 
GACACCACTG TCGAGCACAT CTCTGCCGCC CTGCAGAAGA ACAACACACC CATCTTCGGT 
ATCTGCTTGG GTCACCAGCT GCTCGCTCGT GCTGCCGGTG CCAAGACGGT CAAGCTCAAG 
TTCGGTAACC GTGGCCACAA CATTCCTTGC ACCAGCATGG TCACCGGAAA GTGCCATATC 
ACCTCGCAGA ACCACGGTTA TGCTGTTGAC GCTACCACTC TCCCCACCGG ATGGCAGGAG 
CTGTTCATCA ACGCCAATGA TGGCAGCAAC GAGGGTATCA TGCACGTCGA CCGCCCCCAC 
TTCAGTGTCC AGTTCCACCC TGAGAGCACC CCCGGCCCCC GCGACACCGA GTTCCTCTTT 
GATGTCTTCA TTCAGACTGT TGCCAAGTGC ACTACCGATA ACACTCTCCT CCAGAAGGGT 
GTCGAATTCC CCGGTGGAAC CACTGAGGAG AATGAGAGAC TCCACCCCCG TGTCGATGTC 
AAGAAGGTCC TCGTCTTGGG CAGTGGTGGT CTCAGCATCG GTCAGGCTGG TGAATTCGAC 
TACTCGGGAT CTCAGGCCAT CAAGGCTTTG AAGGAAGAAG GTATCTACAC TGTCCTCATC 
AACCCCAACA TTGCCACCAT TCAGACCTCC AAGGGCTTGG CCGACAAGGT CTACTTCTTG 
CCAGTAAACG CCGAGTTCGT CCGGAAAGTT ATCAAGTACG AACAGCCCGA TGCCATCTAC 
TGCACCTTTG GTGGCCAAAC CGCTCTCTCG GTCGGTATCC AGCTCAAGGA TGAGTTTGAG 
GCTCTTGGTG TCAAGGTTCT CGGTACCCCC ATTGATACCA TCATCACCAC GGAGGACCGT 
GAGCTCTTTG CTCGCAGCAT GGAGTCCATT GGTGAGAAGT GTGCCAAGTC CGCTTCGGCC 
AGCAGTGTTG AGGAGGCCCT CAACGCGGTC AAGGACATTG GTTACCCGGT TATTGTTCGT 
GCAGCCTACG CTCTTGGTGG TCTCGGCAGT GGCTTCGCCA ACAACGAAGC TGAGCTCGTT 
GACCTGTGCA ACAAGGCGCT GGCAGCCAGT CCCCAGGTTC TTGTCGAGCG CAGTATGAAG 
GGCTGGAAGG AGATCGAGTA CGAGGTTGTT CGTGACGCCC AGGACAACTG TATCACTGTG 
TGCAACATGG AGAACTTCGA TCCTCTTGGC ATTCATACCG GCGATTCCAT TGTCGTCGCG 
CCTTCCCAGA CGCTTTCCGA TGAAGACTAC AACATGCTTC GCACTACTGC TGTCAACGTC 
ATTCGCCATC TTGGAGTTGT CGGCGAGTGT AACATCCAGT ATGCTCTGAA CCCCTTCTCC 
AGGGAGTACT GCATTATCGA AGTCAATGCC AGATTGTCGC GTTCCTCGGC CTTGGCTTCC 
AAGGCTACCG GATACCCCTT GGCTTTCATT GCTGCCAAGT TGGGTCTCGG TATTCCTCTG 
AAGGAGATTA AGAACACCGT TACCAAGGTG ACCTGCGCTT GCTTCGAGCC TTCTCTGGAT 
TATGTTGTGG TCAAGATGCC TCGTTGGGAT TTGAAGAAGT TCACCCGCGT CTCCACTCAG 
CTCGGCTCTT CCATGAAGAG TGTCGGCGAG GTCATGAGCA TCGGCAGGAC ATTTGAAGAG 
GCCATCCAGA AGGCTATTCG TGCCATCGAT TTCCACAACC TTGGCTTCAG CGAGTCCAAG 
GGTGCGCTCA TGTCCGTCGA CGACGAGCTC CAGACCCCCT CTGACCAGCG TCTATTCGCC 
ATTGCCAACG CCATGCACGC GGGCTACACT GTCGACAGAA TCTGGGAATT GACCAAGATC 
GACAAGTGGT TCTTGTCCAA GCTCAAGGGC CTGAGCAATT TCGCCAAGGA CATGACCAAA 
CTCACGGCTA ACGATGTTGT TGGTCGTCCT GATCTGCTCC TTCGCGCGAA GCAGCTTGGC 
TTCTGCGACC GTCAGCTTGC CAACTTCTGG GATTCCAACG AGCTAGCTAT TCGTCGCATG 
CGTCTTGAGG CAGGAATCAC TCCTTTCGTC AAGCAGATCG ACACCGTCGC CGCTGAATTC 
CCTGCCTTCA CCAACTACCT GTATCTGACC TACAACGCCA GCGAGCACGA TGTTTCGTTC 
GAAGACCGTG GTGTCATGGT TCTTGGTTCG GGTGTGTACC GCATTGGTTC TTCCGTCGAA 
TTTGATTGGT GTTCCGTTCG GGCCATTCGC ACGCTCCGCG AGTCTGGCTT CAAGACCATC 
ATGGTGAACT ACAACCCTGA GACCGTCAGT ACCGACTATG ATGAAGCCGA CAAGCTGTAC 
TTCGAGAACA TCAACCTGGA GACCGTTCTG GACGTCTATC AGATGGAGGA CGCAACCGGA 
GTGTTGGGAG CTATGGGTGG CCAAACCCCC AACAACATTG CTCTGCCCCT TCTTCGTGCT 
GGCGTCAGAG TTCTGGGTAC TTCTCCTGAG ATGATTGACA CTGCCGAGAA CCGCTACAAG 
TTCTCCCGCA TGCTTGACCG TATCGGTGTC GACCAGCCGA CTTGGAAGGA ACTTACCAGC 
TTCGATGAGG CGAAGGCCTT CTGCCAGAAG GTATCGTACC CGGTGCTGGT CAGACCTTCC 
TATGTGCTTT CTGGCGCTGC CATGAACACT GTCTACTCCG AGGCCGATCT TGAGTCCTAC 
CTTCAGCAGG CTGCCGATGT CTCTCCTGAG CACCCTGTCG TCATTACCAA GTACATCGAG 
AACGCCAAGG AAATCGAGAT GGACGCTGTG GCCAAGGATG GAAAGGTTGT TGGACATTTC 
ATTTCTGAAC ACGTTGAGAA CGCCGGTGTT CACTCTGGTG ATGCAACCCT TATCCTGCCG 
CCTCAGGATC TGGAGCAGAC CACTATCCAG CGCATCGAGG AAGCCACTCG CAAGATCGGT 
GCCGCTCTCA ACGTTACCGG CCCCTTCAAC ATCCAGTTCA TCGCCAAGGA CAACGACATC 
AAGGTGATTG AGTGCAACGT TCGCGCTTCG CGCTCTTTCC CCTTCGTCTC CAAGGTTATG 
GGAGTTGATC TGATCGAGAT GGCCACCAAG GCTATCATGG GTCTGCCTTT CGTAGAGTAC 
CCCGCCATCG ATCGTCCTTC TGGCGGTGTC GGTGTCAAGG TCCCCCAGTT CAGTTTCTCT 
CGTCTGTCTG GCGCTGACCC CGTCTTGGGT GTTGAGATGG CTTCCACTGG TGAAGTTGCC 
TCCTTTGGTG TTGACAAGTA CGAGGCCTAC CTGAAGGCTT TGATGTCCAC CGGCTTCAAG 
GTTCCCAAGA AGAACATTTT GCTCTCCATC GGTTCATACA AGGACAAGAA GGAGATGCTG 
CCTTCTGTGG CCAAGCTTTC TCAGATGGGC TACAAACTCT TCGCTACCGC TGGTACCGCC 
GATTTCCTTC AAGAGCACGA CATCCCCGTG CAATTCTTGG AGGTTCTCGC CAAGGAGGAC 
GATCAGAAGT CCGAGTACTC CCTCACCCAG CACTTGGCTA ACAACATGAT CGACCTGTAC 
ATCAACCTGC CCTCCAACAA CAGATACAGA CGCCCTGCCA ACTACATCAG CAAGGGTTAC 
CAGACACGTC GCATGGCTGT TGATTACCAG ATTCCCTTGG TCACCAACGT GAAGAACGCC 
AAGATCCTCG TCGAGGCTAT CGCCAGGTAC CGTGACATGG AGATCGGCGA GCGTGACTAC 
CAGACTAGCC ATACCCCACT TCAGCTCTCT GGGCAGGTCA ACTTCACCCT TCAGGACTCG 
CTCTCCAGGC CTCACTCTTT CAAGAAAGCC CACGTTCTCT CCGTAGAGCA GTACACTCGC 
GCCGACTTGC ATCTTCTTTT CACTGTTGCC GAGGAGATGC GCCTCAGTGT CCAGCGCGGA 
AACGTCATGG ACATTCTTAA GGGACGCATG CTTGCCACTC TCTTCTACGA GCCTTCCACC 
CGTACGTCGG CGTCTTTCGA GGCGGCTATG AAGCGTCTGG GCGGTGAAGT TATTTCCATC 
GCTACGCAAC ACTCCTCTGT TCAGAAGGGC GAGACTCTGC AGGACACGCT CCGTACTCTT 
GCCTGCTACG CCGACGCCAT TGTTCTCCGC CATCCCGACG AGACCTGCGT TGACGTTGCC 
AAGAAGTACA GCCCGGTGCC TGTTGTCAAC GCTGGTAACG GAAGCAGGGA GCACCCCACC 
CAGGCTTTCT TGGATCTCTT CACTGTCCGT GAGGAGCTGG GCACTGTCCA GGGCTTGACC 
TTCACGTTCG TCGGTGACCT TCGCTATGGC AGACCTGTGC ATTCCCTGGT CTACTTGCTT 
CGTCACTATT CGGGAGTCAA GGTGCAGCTC GTCTCCCCCA AGGGACTTGA GTTGCCTACC 
GATGTTCGTC AGCAACTGGT CAAGGCCGGC CAGCTGCTCT GCGAGTCTGA GACGTTGACC 
CCTGAGATTC TCGGCAAGAC TGATGTCTTG TACTGCACTC GTGTGCAAAA GGAGAGGTTC 
CCGAGCGAGG CCGAGTATGA GAAGGTGAAG GGTTCTTACC GCATCGACAA CCAGACCCTT 
AAGCACGCCA AGAGCAAGAT GGCTATCATG CACCCGCTCC CCAGAAACGA GGAGATTGCT 
GAGGAGGTTG ACTTCGACCA GCGGGCCGCA TACTTCAGAC AGATGAGATA TGGTCTCTAC 
TGCCGTATGG CCCTCCTGGC GTTGGTCATG TCTGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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pyr-3 cDNA ORF clone, Neurospora crassa OR74A

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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