ORF » Species Summary » Neurospora crassa OR74A » NCU05120 cDNA ORF clone

NCU05120 cDNA ORF clone, Neurospora crassa OR74A

The following NCU05120 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU05120 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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ONf10289 XM_952013.3
Latest version!		 Neurospora crassa OR74A DNA-dependent RNA polymerase II RPB140 (NCU05120), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    NCU05120 ( XM_952013.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_957106.3 Neurospora crassa OR74A DNA-dependent RNA polymerase II RPB140 (NCU05120), mRNA.
    Price & Availability↑
    CloneID ONf10289
    Clone ID Related Accession (Same CDS sequence) XM_952013.3
    Accession Version XM_952013.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product DNA-dependent RNA polymerase II RPB140
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506). On Feb 23, 2015 this sequence version replaced XM_952013.2. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCGCCT ACGCAAAGGA CGGCAGCCAG AGCTATGGCG GCTATGGAGA CACATACGAC 
    AATGATGACA ACTACGAGAA CCTCTCCACT TTCGACGACG AAGATGACGG TTCCGACCCC 
    AACGCCATCA CGCCCGAGGA TTCGTGGGAG GTCATCTCTT CCTACTTCGA CCTCAAGGGC 
    CTCGTGTCCC AGCAGATCGA TTCTTTCAAC GAGTTTACAA CAGACACAGT GCAATCTCTA 
    ATAGACGAGT ATGCCGATCT TACCTTGGAC CACCCAAGCC CAGGCGACGA CGAGGGCAGA 
    GAGATCATTT TGCGCAGATA CGACGTTCAT TTCGGAGAGA TCATCATTTC CAGGCCGACC 
    CTCACCGAAG CCACGGGCGA GACTATTAGT CTTCTCCCGT ACGAATGCCG TGACCGCAAC 
    TTGACCTACT CGGCTCCCAT CTACTGCAAG GTCACCAAGC GCGCCCGTAT CGCCGTCAGC 
    GAAAAGAAGC CCCTCAACCA GCTGACCGAT GACGAGCACG ACCACATGGC CCGCACCGGC 
    GAGCACCCGA CTGCTCTGCG CTGGATCGAG GAGGAGAGTG CGCCACCAGA ACCACCCAAG 
    GACGACAAGA TTAACCCGGA AAACCTCAAG GGCTATCTCT TCCTTGGCAA GATCCCTATC 
    ATGGTCAAGT CCAAGATCTG CCACCTTTAC ATGGAGGACG ACGAGTCCTT GTTCACCCTC 
    AACGAGTGTC CTTACGACCA GGGTGGCTAC TTTATCATCA ACGGTAGTGA AAAGGTTTTG 
    ATTGCCCAAG AGCGCTCTGC CGCCAACATT GTCCAGGTCT TTAAGAAGCC TCCTGGTGGC 
    AGCGTCTCGT ACCAGGCCGA GATCCGCAGT GCTCTCGAGA AGGGCTCCCG TCTCATTTCC 
    TCCCTCCAGA TGAGGCTGCA CACAAAGGCT GACCAGAACA AGGGTCGTTT GGCCAACACT 
    GTGAGCGTCA CCCTGCCCTA CGTCAAGGAG GAGGTCTCTC TCGCCATTGT GTTCCGTGCT 
    TTGGGAGTTG TCTCGGACGA GGATATCCTC AACCACATCT GCTACGACCG CCGCGACAGT 
    CAAATGCTCG AAGCCCTTCG TCCCTGCATT GAGGAAGCCT TCTGCATTCA GGACCGTGAG 
    GTCGCTCTGG ATTACATTGG CAAGCGTGGT AACGGCAGCA CCGGAATAAA CCGCAACGGC 
    CGTATCAAGG CTGCTAAGGA GATGCTCCAG AAGGAACTGC TACCCCATAT CTCTCAGGCT 
    GAAGGTTGCG AAACCAGAAA GGCCTTCTTC CTTGGCTACA TGGTTCACAA GCTGCTCCAG 
    TGTGTCCTTG GCCGCAGAGA TACCGATGAT CGTGATCACT TTGGTAAGAA GCGCCTGGAC 
    TTGGCTGGTC CTCTGCTGGC CAAGCTTTTC CGTGGCATCG TGAGACGCCT GACTCAGGAT 
    CTCATGGGCT ACATGAAGCG CTGCGTTGAT ACCAACAAGC ACTTCTCCCT GGCCCTCGGT 
    ATCAAGGCCT CCACCCTTAC CAACGGTCTC AAGTACTCGC TTGCCACTGG TAACTGGGGT 
    GACCAGAAGA AGGCCATGTC CTCGACTGCT GGTGTGTCCC AGGTCTTGAA CAGATACACA 
    TTCTCGTCGA CCCTGTCGCA TTTGCGTCGT ACAAACACGC CCATTGGTCG TGACGGTAAG 
    CTCGCCAAGC CTCGTCAGCT GCACAATACA CACTGGGGTT TGGTCTGCCC AGCAGAAACG 
    CCAGAAGGTC AGGCTTGCGG TCTGGTCAAG AACTTGTCTC TGATGTGCTT CGTCTCGGTG 
    GGTACACTGG CCGATCCCAT CATCGAGTTC ATGATTGCCC GAAACATGGA AGTGTTGGAG 
    GAGTACGAGC CTTTGCGGTA TCCCAACGCC ACCAAGGTGT TCGTCAACGG TACCTGGGTT 
    GGTGTCCATC AAGATCCCAA GCATCTCGTC ACTCTTGTCC AGAACCTCAG ACGCAAGAAC 
    ATCATTTCGT TCGAGGTTTC GCTGGTTCGC GACATCCGTG ACAGAGAGTT CAAGATTTTC 
    TCGGATGCCG GTAGAGTCAT GAGGCCGTTG TTTGTTGTCG AGCAAGAAGA CAACAGCGAA 
    TCCGGTGTCG AAAAGGGTCA GCTCATCCTT ACCAAGGAGC ATATCAGGCG CCTTGAGCAG 
    GATAAGGAGC TTGGCAAATA CCATGAGGAT TACTTTGGCT GGCAAGGCCT GCTCGAGTCA 
    GGTGCTATCG AATACCTCGA CGCCGAGGAG GAAGAGACGG CCATGATCTG CATGACGCCA 
    GAAGATCTGG ACACTTACCG TCAGCACAAG TTCAAGGGTC GCGAGTTTGA GGACGACGCC 
    GCCAACCGTG GCAATAGCCG TATCAAGACA AAATTCAACC CGACAACACA CATGTACACG 
    CACTGCGAGA TTCACCCCAG TATGCTTCTC GGTATCTGCG CCAGTATCAT TCCTTTCCCC 
    GATCACAACC AGTCGCCCAG AAACACTTAC CAGTCTGCCA TGGGTAAGCA AGCTATGGGC 
    TTCTTCCTTA CCAACTACTC CCGACGTATG GATACCATGG CCAACATTCT TTACTACCCT 
    CAAAAACCAC TGGCGACCAC CCGCTCTATG GAATATCTCA AGTTCCGTGA GCTTCCTGCC 
    GGTCAGAACG CCATTGTTGC CATCGCTTGT TACTCTGGTT ACAACCAGGA AGATTCCGTC 
    ATTATGAACC AGAGCAGTAT CGACCGTGGT ATCTTCCGCA GTCTTTTCTT CCGTTCCTAC 
    ACGGATTGCG AGAAGCGTGT CGGCATCAAC ATCGTGGAGC AATTTGAGAA GCCAGATAGA 
    AGCAACACCC TGCGGCCCAA GCACGGCACG TACGACAAGC TTGAGAATGA TGGTATCATT 
    GCTCCTGGCA TAAGAGTCAC CGGTGACGAC ATCATCATCG GCAAGACATC TCCCATCAAC 
    CCCGATAACC AAGAACTTGG CCAGCGCACG GCTCAGCACG TCAAGCGCGA CGCGTCCACG 
    CCGCTGCGCA GCACGGAGAG TGGTATCGTC GATTCCGTGG CCTTTACAAC CAACCAGGAT 
    GGTTTGCGCT ACGTAAAGGT CAGGGTTCGT ACGACAAAGA TTCCCCAGAT TGGTGATAAG 
    TTTGCGTCTC GTCACGGTCA AAAGGGTACC ATTGGTGTTA CTTACCGTCA AGAGGATATG 
    CCGTTTACCG CCGAGGGTAT CACGCCTGAT ATTATCATCA ACCCTCACGC CATTCCTTCG 
    CGTATGACAA TTGCCCATTT GATCGAGTGT CTGCTGTCCA AGGTCGGTGC GCTCAAGGGC 
    ATGGAGGGTG ATGCCACCCC CTTCACGGAT GTCACGGTCG ATTCCGTCTC CAACCTCCTC 
    CGCGAGCACG GCTACCAATC CCGTGGCTTT GAAATCATGT ACCACGGCCA TACCGGCAAG 
    AAGCTTCGCG CCCAGGTGTT CTTCGGTCCT ACGTACTACC AGCGTTTGAG GCATATGGTG 
    GACGACAAGA TCCACGCTCG TGCTCGTGGT CCGGTGCAGA TCATGACTCG TCAGCCCGTC 
    GAAGGTCGTG CCAGAGACGG TGGTCTCCGT TTCGGTGAAA TGGAACGTGA CTGTATGATT 
    GCTCACGGTG CCGCTGCCTT CTTGAAGGAG AGATTGTTCG AGGTTTCGGA TGCTTTTAGA 
    GTGCACATTT GCGAGATTTG CGGTCTCATG ACTCCCATTG CAAACCTCAC CAAGCAATCG 
    TTCGAATGCC GTCCTTGCAA GAACAAGACC AAGATCGCCC AGGTCCACAT GCCCTACGCG 
    GCCAAGCTTC TGTTCCAGGA GCTCATGTCG ATGAACATTG CCTCGCGCTT GTTCACCAGC 
    CGGTCCGGCA TCTCGGTTCG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_957106.3
    CDS645..4508
    Translation

    Target ORF information:

    RefSeq Version XM_952013.3
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A DNA-dependent RNA polymerase II RPB140 (NCU05120), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_952013.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCGCCT ACGCAAAGGA CGGCAGCCAG AGCTATGGCG GCTATGGAGA CACATACGAC 
    AATGATGACA ACTACGAGAA CCTCTCCACT TTCGACGACG AAGATGACGG TTCCGACCCC 
    AACGCCATCA CGCCCGAGGA TTCGTGGGAG GTCATCTCTT CCTACTTCGA CCTCAAGGGC 
    CTCGTGTCCC AGCAGATCGA TTCTTTCAAC GAGTTTACAA CAGACACAGT GCAATCTCTA 
    ATAGACGAGT ATGCCGATCT TACCTTGGAC CACCCAAGCC CAGGCGACGA CGAGGGCAGA 
    GAGATCATTT TGCGCAGATA CGACGTTCAT TTCGGAGAGA TCATCATTTC CAGGCCGACC 
    CTCACCGAAG CCACGGGCGA GACTATTAGT CTTCTCCCGT ACGAATGCCG TGACCGCAAC 
    TTGACCTACT CGGCTCCCAT CTACTGCAAG GTCACCAAGC GCGCCCGTAT CGCCGTCAGC 
    GAAAAGAAGC CCCTCAACCA GCTGACCGAT GACGAGCACG ACCACATGGC CCGCACCGGC 
    GAGCACCCGA CTGCTCTGCG CTGGATCGAG GAGGAGAGTG CGCCACCAGA ACCACCCAAG 
    GACGACAAGA TTAACCCGGA AAACCTCAAG GGCTATCTCT TCCTTGGCAA GATCCCTATC 
    ATGGTCAAGT CCAAGATCTG CCACCTTTAC ATGGAGGACG ACGAGTCCTT GTTCACCCTC 
    AACGAGTGTC CTTACGACCA GGGTGGCTAC TTTATCATCA ACGGTAGTGA AAAGGTTTTG 
    ATTGCCCAAG AGCGCTCTGC CGCCAACATT GTCCAGGTCT TTAAGAAGCC TCCTGGTGGC 
    AGCGTCTCGT ACCAGGCCGA GATCCGCAGT GCTCTCGAGA AGGGCTCCCG TCTCATTTCC 
    TCCCTCCAGA TGAGGCTGCA CACAAAGGCT GACCAGAACA AGGGTCGTTT GGCCAACACT 
    GTGAGCGTCA CCCTGCCCTA CGTCAAGGAG GAGGTCTCTC TCGCCATTGT GTTCCGTGCT 
    TTGGGAGTTG TCTCGGACGA GGATATCCTC AACCACATCT GCTACGACCG CCGCGACAGT 
    CAAATGCTCG AAGCCCTTCG TCCCTGCATT GAGGAAGCCT TCTGCATTCA GGACCGTGAG 
    GTCGCTCTGG ATTACATTGG CAAGCGTGGT AACGGCAGCA CCGGAATAAA CCGCAACGGC 
    CGTATCAAGG CTGCTAAGGA GATGCTCCAG AAGGAACTGC TACCCCATAT CTCTCAGGCT 
    GAAGGTTGCG AAACCAGAAA GGCCTTCTTC CTTGGCTACA TGGTTCACAA GCTGCTCCAG 
    TGTGTCCTTG GCCGCAGAGA TACCGATGAT CGTGATCACT TTGGTAAGAA GCGCCTGGAC 
    TTGGCTGGTC CTCTGCTGGC CAAGCTTTTC CGTGGCATCG TGAGACGCCT GACTCAGGAT 
    CTCATGGGCT ACATGAAGCG CTGCGTTGAT ACCAACAAGC ACTTCTCCCT GGCCCTCGGT 
    ATCAAGGCCT CCACCCTTAC CAACGGTCTC AAGTACTCGC TTGCCACTGG TAACTGGGGT 
    GACCAGAAGA AGGCCATGTC CTCGACTGCT GGTGTGTCCC AGGTCTTGAA CAGATACACA 
    TTCTCGTCGA CCCTGTCGCA TTTGCGTCGT ACAAACACGC CCATTGGTCG TGACGGTAAG 
    CTCGCCAAGC CTCGTCAGCT GCACAATACA CACTGGGGTT TGGTCTGCCC AGCAGAAACG 
    CCAGAAGGTC AGGCTTGCGG TCTGGTCAAG AACTTGTCTC TGATGTGCTT CGTCTCGGTG 
    GGTACACTGG CCGATCCCAT CATCGAGTTC ATGATTGCCC GAAACATGGA AGTGTTGGAG 
    GAGTACGAGC CTTTGCGGTA TCCCAACGCC ACCAAGGTGT TCGTCAACGG TACCTGGGTT 
    GGTGTCCATC AAGATCCCAA GCATCTCGTC ACTCTTGTCC AGAACCTCAG ACGCAAGAAC 
    ATCATTTCGT TCGAGGTTTC GCTGGTTCGC GACATCCGTG ACAGAGAGTT CAAGATTTTC 
    TCGGATGCCG GTAGAGTCAT GAGGCCGTTG TTTGTTGTCG AGCAAGAAGA CAACAGCGAA 
    TCCGGTGTCG AAAAGGGTCA GCTCATCCTT ACCAAGGAGC ATATCAGGCG CCTTGAGCAG 
    GATAAGGAGC TTGGCAAATA CCATGAGGAT TACTTTGGCT GGCAAGGCCT GCTCGAGTCA 
    GGTGCTATCG AATACCTCGA CGCCGAGGAG GAAGAGACGG CCATGATCTG CATGACGCCA 
    GAAGATCTGG ACACTTACCG TCAGCACAAG TTCAAGGGTC GCGAGTTTGA GGACGACGCC 
    GCCAACCGTG GCAATAGCCG TATCAAGACA AAATTCAACC CGACAACACA CATGTACACG 
    CACTGCGAGA TTCACCCCAG TATGCTTCTC GGTATCTGCG CCAGTATCAT TCCTTTCCCC 
    GATCACAACC AGTCGCCCAG AAACACTTAC CAGTCTGCCA TGGGTAAGCA AGCTATGGGC 
    TTCTTCCTTA CCAACTACTC CCGACGTATG GATACCATGG CCAACATTCT TTACTACCCT 
    CAAAAACCAC TGGCGACCAC CCGCTCTATG GAATATCTCA AGTTCCGTGA GCTTCCTGCC 
    GGTCAGAACG CCATTGTTGC CATCGCTTGT TACTCTGGTT ACAACCAGGA AGATTCCGTC 
    ATTATGAACC AGAGCAGTAT CGACCGTGGT ATCTTCCGCA GTCTTTTCTT CCGTTCCTAC 
    ACGGATTGCG AGAAGCGTGT CGGCATCAAC ATCGTGGAGC AATTTGAGAA GCCAGATAGA 
    AGCAACACCC TGCGGCCCAA GCACGGCACG TACGACAAGC TTGAGAATGA TGGTATCATT 
    GCTCCTGGCA TAAGAGTCAC CGGTGACGAC ATCATCATCG GCAAGACATC TCCCATCAAC 
    CCCGATAACC AAGAACTTGG CCAGCGCACG GCTCAGCACG TCAAGCGCGA CGCGTCCACG 
    CCGCTGCGCA GCACGGAGAG TGGTATCGTC GATTCCGTGG CCTTTACAAC CAACCAGGAT 
    GGTTTGCGCT ACGTAAAGGT CAGGGTTCGT ACGACAAAGA TTCCCCAGAT TGGTGATAAG 
    TTTGCGTCTC GTCACGGTCA AAAGGGTACC ATTGGTGTTA CTTACCGTCA AGAGGATATG 
    CCGTTTACCG CCGAGGGTAT CACGCCTGAT ATTATCATCA ACCCTCACGC CATTCCTTCG 
    CGTATGACAA TTGCCCATTT GATCGAGTGT CTGCTGTCCA AGGTCGGTGC GCTCAAGGGC 
    ATGGAGGGTG ATGCCACCCC CTTCACGGAT GTCACGGTCG ATTCCGTCTC CAACCTCCTC 
    CGCGAGCACG GCTACCAATC CCGTGGCTTT GAAATCATGT ACCACGGCCA TACCGGCAAG 
    AAGCTTCGCG CCCAGGTGTT CTTCGGTCCT ACGTACTACC AGCGTTTGAG GCATATGGTG 
    GACGACAAGA TCCACGCTCG TGCTCGTGGT CCGGTGCAGA TCATGACTCG TCAGCCCGTC 
    GAAGGTCGTG CCAGAGACGG TGGTCTCCGT TTCGGTGAAA TGGAACGTGA CTGTATGATT 
    GCTCACGGTG CCGCTGCCTT CTTGAAGGAG AGATTGTTCG AGGTTTCGGA TGCTTTTAGA 
    GTGCACATTT GCGAGATTTG CGGTCTCATG ACTCCCATTG CAAACCTCAC CAAGCAATCG 
    TTCGAATGCC GTCCTTGCAA GAACAAGACC AAGATCGCCC AGGTCCACAT GCCCTACGCG 
    GCCAAGCTTC TGTTCCAGGA GCTCATGTCG ATGAACATTG CCTCGCGCTT GTTCACCAGC 
    CGGTCCGGCA TCTCGGTTCG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B


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