rpb-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	rpb-2
Entrez Gene ID	175668
Full Name	DNA-directed RNA polymerase II subunit RPB2
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_065646.4	NP_498047.1	DNA-directed RNA polymerase II subunit RPB2
NM_065646.6	NP_498047.2	DNA-directed RNA polymerase II subunit RPB2
NM_065646.5	NP_498047.2	DNA-directed RNA polymerase II subunit RPB2

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLR2B	NP_000929.1
Rattus norvegicus (Norway rat)	Polr2b	NP_001099472.1
Gallus gallus (chicken)	POLR2B	NP_001006448.1
Drosophila melanogaster (fruit fly)	RpII140	NP_476706.1
Mus musculus (house mouse)	Polr2b	NP_722493.2
Danio rerio (zebrafish)	polr2b	NP_001019632.2
Schizosaccharomyces pombe (fission yeast)	rpb2	NP_593101.2
Magnaporthe oryzae (rice blast fungus)	MGG_04714	XP_003710821.1
Neurospora crassa	NCU05120	XP_957106.2
Arabidopsis thaliana (thale cress)	NRPB2	NP_193902.1
Canis lupus familiaris (dog)	POLR2B	XP_532382.3
Saccharomyces cerevisiae (baker's yeast)	RPB2	NP_014794.3
Kluyveromyces lactis	KLLA0B05577g	XP_451784.1
Eremothecium gossypii	AGOS_AFR404C	NP_985951.1
	POLR2B	XP_001083325.2
Pan troglodytes (chimpanzee)	POLR2B	XP_517275.2
Bos taurus (cattle)	POLR2B	NP_001092552.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003648	XP_313416.4
Caenorhabditis elegans (roundworm)	rpb-2	NP_498047.1
Oryza sativa (rice)	Os03g0646800	NP_001050770.1
Xenopus tropicalis (tropical clawed frog)	polr2b	XP_002937598.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our findings suggest that SIG-7 plays a central role in both Pol II elongation and co-transcriptional splicing and may provide an important link for their coordination and regulation
Title: A Conserved Nuclear Cyclophilin Is Required for Both RNA Polymerase II Elongation and Co-transcriptional Splicing in Caenorhabditis elegans.

The following rpb-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rpb-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa341117	NM_065646.4 Latest version!	Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB2 (rpb-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa698874	NM_065646.6 Latest version!	Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB2 (rpb-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa698874	NM_065646.5	Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB2 (rpb-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rpb-2 ( NM_065646.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498047.1 Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB2 (rpb-2), partial mRNA.

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CloneID	OCa341117
Clone ID Related Accession (Same CDS sequence)	NM_065646.4
Accession Version	NM_065646.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-14
Organism	Caenorhabditis elegans
Product	DNA-directed RNA polymerase II subunit RPB2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_065646.3. COMPLETENESS: incomplete on both ends.

ATGTACGATG ATGAGGATGA GATGGTTAAT GACCCGATGG ACGGAGACTA TATCGATGAT 
TCGGATGAAA TCAGTGCAGA AGCATGGCAA GAAGCATGTT GGGTTGTTAT CAGTGCTTAT 
TTCGATGAAA AGGGTCTCGT TCGTCAACAA CTTGATTCCT TCGATGAATT CGTCCAAATG 
AATGTGCAGC GAATTGTTGA GGATTCTCCG CCAGTCGAAC TTCAATCAGA AAATCAGCAT 
CTTGGCACAG ATATGGAAAA TCCTGCGAAA TTCTCATTGA AATTCAATCA AATTTACCTC 
TCGAAACCAA CTCACTGGGA GAAAGACGGA GCACCAATGC CAATGATGCC TAACGAAGCT 
CGTCTCCGAA ATTTGACATA CGCCTCGCCA CTTTACGTAG ATATCACTAA AGTGGTGACA 
AGAGACGATT CAGCAACGGA AAAAGTGTAC GACAAAGTGT TCGTTGGAAA AGTTCCAGTT 
ATGCTACGGT CTAGTTACTG TATGCTTTCC AATATGACAG ATCGTGATCT AACAGAACTC 
AATGAGTGCC CACTTGATCC TGGAGGTTAT TTTGTTATCA ATGGTTCGGA GAAAGTACTT 
ATTGCTCAAG AAAAGATGGC GACTAATACA GTTTACGTGT TCTCAATGAA AGACGGAAAA 
TATGCATTCA AAACGGAATG TCGCTCTTGT TTGGAAAATT CATCTCGTCC GACATCCACA 
ATGTGGGTTA ATATGCTTGC AAGAGGAGGA GGTGGTGGAA AGAAGACGGC GATGGGTCAA 
CGAATTATTG GAATTTTACC ATATATTAAA CAAGAAATTC CGATTATGAT TGTGTTCCGA 
GCGCTGGGAT TCGTATCAGA TCGTGATATT CTTGGACACA TTATCTACGA TTTCAATGAT 
CCAGAAATGA TGGAAATGGT CAAACCTTCG TTAGATGAAG CTTTTGTCAT TCAGGAGCAA 
AATGTTGCTC TCAACTTTAT CGGAGCTCGT GGAGCAAAAC CAGGAGTAAC CAGAGAACAA 
AGAATCAAAT ATGCTCGTGA GATTCTTCAA AAAGAACTTT TGCCTCATGT TGGTGTATCT 
GAGCATTGCG AAACGAAAAA AGCATTCTTC ATCGGATACA TGGTGCATCG TCTTCTTCTA 
GCCGCCCTCG GACGTCGTGA ACTTGATGAT CGTGATCACA TTGGAAACAA AAGACTGGAT 
TTAGCCGGAC CACTTCTTGC TTTCTTGTTC CGTTCCCTTT TCAGAAATCT TCTCAAGGAG 
ATGAGAATGA CAGCTCAAAA GTATATCAAC AAGAATGACG ACTTTGCTCT TGATGTCTGC 
GTCAAAACTT CAACAATAAC TCGTGGATTG ACATATTCAT TGGCTACCGG TAATTGGGGT 
GATCAAAAGA AAGCACATCA ATCGAGAGCC GGTGTGTCTC AAGTACTCAA TCGTCTCACC 
TACACGGCCA CACTTTCACA TTTGCGTCGA GCGAACTCTC CTATTGGACG AGAAGGAAAA 
CTTGCCAAGC CTCGTCAGCT GCATAATACT CAATGGGGAA TGGTGTGTCC TGCTGAAACT 
CCTGAAGGAC AGGCTGTAGG TTTGGTGAAG AATCTGGCAC TTATGGCATA CATCTCTGTC 
GGTTCGTTAC CAGAGCCAAT TCTGGAATTC TTGGAAGAAT GGTCAATGGA AAATCTTGAA 
GAAGTGTCTC CATCGGCTAT TGCCGATGCT ACGAAGATCT TTGTCAACGG TGCATGGGTG 
GGAATTCATC GTGAACCCGA TCAATTGATG ACGACGTTGA AGAAGCTTCG ACGTCAAATG 
GACATCATCG TTTCCGAAGT ATCAATGGTT CGTGATATTC GTGACAGAGA AATTCGTATC 
TACACTGATG CTGGAAGAGT TTGCCGTCCA TTACTTATTG TTGAGAATCA GAAATTGGCA 
TTGAAGAAGA GACACATTGA TCAGTTGAAG GAGGCTGCTG ACGAAGCGAA TAAATACACA 
TGGTCTGATC TTGTTGGTGG TGGAGTTGTC GAACTGATTG ATTCAATGGA AGAAGAAACA 
TCAATGATTG CAATGATGCC AGAAGATTTG AGAAGTGGTG GTTACTGTGA CACTCATACT 
CATTGTGAAA TTCATCCAGC CATGATTCTC GGAGTTTGTG CCTCAATCAT TCCATTCCCA 
GATCATAATC AATCACCACG TAATACATAC CAAAGTGCTA TGGGTAAGCA AGCTATGGGA 
GTTTATACAA CAAATTTCCA TGTTCGAATG GACACTCTTG CACACGTTCT CTACTATCCA 
CAAAAACCGC TTGTCACTAC AAGATCTATG GAATATCTTC GATTCAATGA ACTTCCAGCC 
GGAATCAACG CAATTGTTGC TATTCTTTCA TATTCCGGAT ATAATCAGGA AGATTCTGTC 
ATTATGAACA ACTCTGCAAT TGATCGAGGA CTTTTCCGTT CTGTTTTCTA TCGTTCTTAT 
CGTGACAATG AGGCAAATCT TGACAATGCA AATGAAGAAC TGATTGAGAA ACCAACTCGT 
GAAAAATGTT CTGGAATGCG TCATTCATTG TACGATAAGC TTGATGAAGA CGGAATCATC 
TCTCCAGGAA TGCGTGTGTC TGGCGATGAC GTCATTATTG GAAAAACAGT TGCTCTGCCA 
GATATTGATG ATGATTTGGA TGCATCTGGG AAGAAATATC CAAAGAGAGA TGCATCGACA 
TTCTTGAGAA GTTCTGAAAC TGGTATTGTG GATCAGGTGA TGCTCTCCCT CAACAGCGAT 
GGAAATAAAT TTGTCAAGAT TCGTATGCGT TCTGTTCGTC TTCCACAAAT TGGAGATAAA 
TTCGCATCTC GACACGGTCA AAAAGGAACC ATGGGTATTA TGTATCGGCA AGAAGATATG 
CCATTTACTG CTGAAGGACT CACTCCTGAT ATCATCATTA ATCCTCATGC CGTTCCATCT 
CGTATGACAA TTGGTCACTT GATCGAATGC CTTCAGGGTA AACTATCAGC AAACAAGGGA 
GAAATCGGAG ATGCTACACC ATTCAACGAT ACAGTCAACG TACAAAAGAT CTCTGGCCTA 
CTATGTGAGT ATGGTTATCA TTTGCGTGGT AACGAAGTGA TGTACAACGG ACATACTGGA 
AAAAAGTTGA CAACTCAAAT CTTCTTTGGA CCAACTTATT ATCAACGTCT CAAGCATATG 
GTCGATGACA AGATTCACTC ACGTGCACGC GGTCCAATTC AGATGATGAA TCGACAGCCT 
ATGGAAGGAA GAGCTCGTGA CGGAGGTCTC CGTTTCGGAG AAATGGAACG AGATTGTCAA 
ATTTCCCACG GAGCTACTCA ATTTTTGCGA GAACGTCTTT TTGAGGTGTC AGATCCATAT 
CATGTGTACG TGTGCAACAA CTGTGGTCTT ATTGTCGTCG CCAACTTGAG AACGAACAGT 
TTCGAGTGCA AGGCTTGTCG CAACAAGACC CAAGTATCCG CTGTTCGTAT TCCATACGCT 
TGTAAGCTGC TCTTCCAGGA GTTAATGTCA ATGTCTATTG CTCCAAGACT CATGGTCAAG 
CCTCGACAAT CAAAACGTTC AAAACATCAA TCGGAAGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498047.1
CDS	1..3582
Translation	MYDDEDEMVNDPMDGDYIDDSDEISAEAWQEACWVVISAYFDEKGLVRQQLDSFDEFVQMNVQRIVEDSPPVELQSENQHLGTDMENPAKFSLKFNQIYLSKPTHWEKDGAPMPMMPNEARLRNLTYASPLYVDITKVVTRDDSATEKVYDKVFVGKVPVMLRSSYCMLSNMTDRDLTELNECPLDPGGYFVINGSEKVLIAQEKMATNTVYVFSMKDGKYAFKTECRSCLENSSRPTSTMWVNMLARGGGGGKKTAMGQRIIGILPYIKQEIPIMIVFRALGFVSDRDILGHIIYDFNDPEMMEMVKPSLDEAFVIQEQNVALNFIGARGAKPGVTREQRIKYAREILQKELLPHVGVSEHCETKKAFFIGYMVHRLLLAALGRRELDDRDHIGNKRLDLAGPLLAFLFRSLFRNLLKEMRMTAQKYINKNDDFALDVCVKTSTITRGLTYSLATGNWGDQKKAHQSRAGVSQVLNRLTYTATLSHLRRANSPIGREGKLAKPRQLHNTQWGMVCPAETPEGQAVGLVKNLALMAYISVGSLPEPILEFLEEWSMENLEEVSPSAIADATKIFVNGAWVGIHREPDQLMTTLKKLRRQMDIIVSEVSMVRDIRDREIRIYTDAGRVCRPLLIVENQKLALKKRHIDQLKEAADEANKYTWSDLVGGGVVELIDSMEEETSMIAMMPEDLRSGGYCDTHTHCEIHPAMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYTTNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFNELPAGINAIVAILSYSGYNQEDSVIMNNSAIDRGLFRSVFYRSYRDNEANLDNANEELIEKPTREKCSGMRHSLYDKLDEDGIISPGMRVSGDDVIIGKTVALPDIDDDLDASGKKYPKRDASTFLRSSETGIVDQVMLSLNSDGNKFVKIRMRSVRLPQIGDKFASRHGQKGTMGIMYRQEDMPFTAEGLTPDIIINPHAVPSRMTIGHLIECLQGKLSANKGEIGDATPFNDTVNVQKISGLLCEYGYHLRGNEVMYNGHTGKKLTTQIFFGPTYYQRLKHMVDDKIHSRARGPIQMMNRQPMEGRARDGGLRFGEMERDCQISHGATQFLRERLFEVSDPYHVYVCNNCGLIVVANLRTNSFECKACRNKTQVSAVRIPYACKLLFQELMSMSIAPRLMVKPRQSKRSKHQSEA

Target ORF information:

RefSeq Version	NM_065646.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB2 (rpb-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065646.4

ORF Insert Sequence:

ATGTACGATG ATGAGGATGA GATGGTTAAT GACCCGATGG ACGGAGACTA TATCGATGAT 
TCGGATGAAA TCAGTGCAGA AGCATGGCAA GAAGCATGTT GGGTTGTTAT CAGTGCTTAT 
TTCGATGAAA AGGGTCTCGT TCGTCAACAA CTTGATTCCT TCGATGAATT CGTCCAAATG 
AATGTGCAGC GAATTGTTGA GGATTCTCCG CCAGTCGAAC TTCAATCAGA AAATCAGCAT 
CTTGGCACAG ATATGGAAAA TCCTGCGAAA TTCTCATTGA AATTCAATCA AATTTACCTC 
TCGAAACCAA CTCACTGGGA GAAAGACGGA GCACCAATGC CAATGATGCC TAACGAAGCT 
CGTCTCCGAA ATTTGACATA CGCCTCGCCA CTTTACGTAG ATATCACTAA AGTGGTGACA 
AGAGACGATT CAGCAACGGA AAAAGTGTAC GACAAAGTGT TCGTTGGAAA AGTTCCAGTT 
ATGCTACGGT CTAGTTACTG TATGCTTTCC AATATGACAG ATCGTGATCT AACAGAACTC 
AATGAGTGCC CACTTGATCC TGGAGGTTAT TTTGTTATCA ATGGTTCGGA GAAAGTACTT 
ATTGCTCAAG AAAAGATGGC GACTAATACA GTTTACGTGT TCTCAATGAA AGACGGAAAA 
TATGCATTCA AAACGGAATG TCGCTCTTGT TTGGAAAATT CATCTCGTCC GACATCCACA 
ATGTGGGTTA ATATGCTTGC AAGAGGAGGA GGTGGTGGAA AGAAGACGGC GATGGGTCAA 
CGAATTATTG GAATTTTACC ATATATTAAA CAAGAAATTC CGATTATGAT TGTGTTCCGA 
GCGCTGGGAT TCGTATCAGA TCGTGATATT CTTGGACACA TTATCTACGA TTTCAATGAT 
CCAGAAATGA TGGAAATGGT CAAACCTTCG TTAGATGAAG CTTTTGTCAT TCAGGAGCAA 
AATGTTGCTC TCAACTTTAT CGGAGCTCGT GGAGCAAAAC CAGGAGTAAC CAGAGAACAA 
AGAATCAAAT ATGCTCGTGA GATTCTTCAA AAAGAACTTT TGCCTCATGT TGGTGTATCT 
GAGCATTGCG AAACGAAAAA AGCATTCTTC ATCGGATACA TGGTGCATCG TCTTCTTCTA 
GCCGCCCTCG GACGTCGTGA ACTTGATGAT CGTGATCACA TTGGAAACAA AAGACTGGAT 
TTAGCCGGAC CACTTCTTGC TTTCTTGTTC CGTTCCCTTT TCAGAAATCT TCTCAAGGAG 
ATGAGAATGA CAGCTCAAAA GTATATCAAC AAGAATGACG ACTTTGCTCT TGATGTCTGC 
GTCAAAACTT CAACAATAAC TCGTGGATTG ACATATTCAT TGGCTACCGG TAATTGGGGT 
GATCAAAAGA AAGCACATCA ATCGAGAGCC GGTGTGTCTC AAGTACTCAA TCGTCTCACC 
TACACGGCCA CACTTTCACA TTTGCGTCGA GCGAACTCTC CTATTGGACG AGAAGGAAAA 
CTTGCCAAGC CTCGTCAGCT GCATAATACT CAATGGGGAA TGGTGTGTCC TGCTGAAACT 
CCTGAAGGAC AGGCTGTAGG TTTGGTGAAG AATCTGGCAC TTATGGCATA CATCTCTGTC 
GGTTCGTTAC CAGAGCCAAT TCTGGAATTC TTGGAAGAAT GGTCAATGGA AAATCTTGAA 
GAAGTGTCTC CATCGGCTAT TGCCGATGCT ACGAAGATCT TTGTCAACGG TGCATGGGTG 
GGAATTCATC GTGAACCCGA TCAATTGATG ACGACGTTGA AGAAGCTTCG ACGTCAAATG 
GACATCATCG TTTCCGAAGT ATCAATGGTT CGTGATATTC GTGACAGAGA AATTCGTATC 
TACACTGATG CTGGAAGAGT TTGCCGTCCA TTACTTATTG TTGAGAATCA GAAATTGGCA 
TTGAAGAAGA GACACATTGA TCAGTTGAAG GAGGCTGCTG ACGAAGCGAA TAAATACACA 
TGGTCTGATC TTGTTGGTGG TGGAGTTGTC GAACTGATTG ATTCAATGGA AGAAGAAACA 
TCAATGATTG CAATGATGCC AGAAGATTTG AGAAGTGGTG GTTACTGTGA CACTCATACT 
CATTGTGAAA TTCATCCAGC CATGATTCTC GGAGTTTGTG CCTCAATCAT TCCATTCCCA 
GATCATAATC AATCACCACG TAATACATAC CAAAGTGCTA TGGGTAAGCA AGCTATGGGA 
GTTTATACAA CAAATTTCCA TGTTCGAATG GACACTCTTG CACACGTTCT CTACTATCCA 
CAAAAACCGC TTGTCACTAC AAGATCTATG GAATATCTTC GATTCAATGA ACTTCCAGCC 
GGAATCAACG CAATTGTTGC TATTCTTTCA TATTCCGGAT ATAATCAGGA AGATTCTGTC 
ATTATGAACA ACTCTGCAAT TGATCGAGGA CTTTTCCGTT CTGTTTTCTA TCGTTCTTAT 
CGTGACAATG AGGCAAATCT TGACAATGCA AATGAAGAAC TGATTGAGAA ACCAACTCGT 
GAAAAATGTT CTGGAATGCG TCATTCATTG TACGATAAGC TTGATGAAGA CGGAATCATC 
TCTCCAGGAA TGCGTGTGTC TGGCGATGAC GTCATTATTG GAAAAACAGT TGCTCTGCCA 
GATATTGATG ATGATTTGGA TGCATCTGGG AAGAAATATC CAAAGAGAGA TGCATCGACA 
TTCTTGAGAA GTTCTGAAAC TGGTATTGTG GATCAGGTGA TGCTCTCCCT CAACAGCGAT 
GGAAATAAAT TTGTCAAGAT TCGTATGCGT TCTGTTCGTC TTCCACAAAT TGGAGATAAA 
TTCGCATCTC GACACGGTCA AAAAGGAACC ATGGGTATTA TGTATCGGCA AGAAGATATG 
CCATTTACTG CTGAAGGACT CACTCCTGAT ATCATCATTA ATCCTCATGC CGTTCCATCT 
CGTATGACAA TTGGTCACTT GATCGAATGC CTTCAGGGTA AACTATCAGC AAACAAGGGA 
GAAATCGGAG ATGCTACACC ATTCAACGAT ACAGTCAACG TACAAAAGAT CTCTGGCCTA 
CTATGTGAGT ATGGTTATCA TTTGCGTGGT AACGAAGTGA TGTACAACGG ACATACTGGA 
AAAAAGTTGA CAACTCAAAT CTTCTTTGGA CCAACTTATT ATCAACGTCT CAAGCATATG 
GTCGATGACA AGATTCACTC ACGTGCACGC GGTCCAATTC AGATGATGAA TCGACAGCCT 
ATGGAAGGAA GAGCTCGTGA CGGAGGTCTC CGTTTCGGAG AAATGGAACG AGATTGTCAA 
ATTTCCCACG GAGCTACTCA ATTTTTGCGA GAACGTCTTT TTGAGGTGTC AGATCCATAT 
CATGTGTACG TGTGCAACAA CTGTGGTCTT ATTGTCGTCG CCAACTTGAG AACGAACAGT 
TTCGAGTGCA AGGCTTGTCG CAACAAGACC CAAGTATCCG CTGTTCGTAT TCCATACGCT 
TGTAAGCTGC TCTTCCAGGA GTTAATGTCA ATGTCTATTG CTCCAAGACT CATGGTCAAG 
CCTCGACAAT CAAAACGTTC AAAACATCAA TCGGAAGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rpb-2 ( NM_065646.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498047.2 Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB2 (rpb-2), mRNA.

Price & Availability↑

CloneID	OCa698874
Clone ID Related Accession (Same CDS sequence)	NM_065646.6 , NM_065646.5
Accession Version	NM_065646.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	DNA-directed RNA polymerase II subunit RPB2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_065646.5.

ATGATGTACG ATGATGAGGA TGAGATGGTT AATGACCCGA TGGACGGAGA CTATATCGAT 
GATTCGGATG AAATCAGTGC AGAAGCATGG CAAGAAGCAT GTTGGGTTGT TATCAGTGCT 
TATTTCGATG AAAAGGGTCT CGTTCGTCAA CAACTTGATT CCTTCGATGA ATTCGTCCAA 
ATGAATGTGC AGCGAATTGT TGAGGATTCT CCGCCAGTCG AACTTCAATC AGAAAATCAG 
CATCTTGGCA CAGATATGGA AAATCCTGCG AAATTCTCAT TGAAATTCAA TCAAATTTAC 
CTCTCGAAAC CAACTCACTG GGAGAAAGAC GGAGCACCAA TGCCAATGAT GCCTAACGAA 
GCTCGTCTCC GAAATTTGAC ATACGCCTCG CCACTTTACG TAGATATCAC TAAAGTGGTG 
ACAAGAGACG ATTCAGCAAC GGAAAAAGTG TACGACAAAG TGTTCGTTGG AAAAGTTCCA 
GTTATGCTAC GGTCTAGTTA CTGTATGCTT TCCAATATGA CAGATCGTGA TCTAACAGAA 
CTCAATGAGT GCCCACTTGA TCCTGGAGGT TATTTTGTTA TCAATGGTTC GGAGAAAGTA 
CTTATTGCTC AAGAAAAGAT GGCGACTAAT ACAGTTTACG TGTTCTCAAT GAAAGACGGA 
AAATATGCAT TCAAAACGGA ATGTCGCTCT TGTTTGGAAA ATTCATCTCG TCCGACATCC 
ACAATGTGGG TTAATATGCT TGCAAGAGGA GGAGGTGGTG GAAAGAAGAC GGCGATGGGT 
CAACGAATTA TTGGAATTTT ACCATATATT AAACAAGAAA TTCCGATTAT GATTGTGTTC 
CGAGCGCTGG GATTCGTATC AGATCGTGAT ATTCTTGGAC ACATTATCTA CGATTTCAAT 
GATCCAGAAA TGATGGAAAT GGTCAAACCT TCGTTAGATG AAGCTTTTGT CATTCAGGAG 
CAAAATGTTG CTCTCAACTT TATCGGAGCT CGTGGAGCAA AACCAGGAGT AACCAGAGAA 
CAAAGAATCA AATATGCTCG TGAGATTCTT CAAAAAGAAC TTTTGCCTCA TGTTGGTGTA 
TCTGAGCATT GCGAAACGAA AAAAGCATTC TTCATCGGAT ACATGGTGCA TCGTCTTCTT 
CTAGCCGCCC TCGGACGTCG TGAACTTGAT GATCGTGATC ACATTGGAAA CAAAAGACTG 
GATTTAGCCG GACCACTTCT TGCTTTCTTG TTCCGTTCCC TTTTCAGAAA TCTTCTCAAG 
GAGATGAGAA TGACAGCTCA AAAGTATATC AACAAGAATG ACGACTTTGC TCTTGATGTC 
TGCGTCAAAA CTTCAACAAT AACTCGTGGA TTGACATATT CATTGGCTAC CGGTAATTGG 
GGTGATCAAA AGAAAGCACA TCAATCGAGA GCCGGTGTGT CTCAAGTACT CAATCGTCTC 
ACCTACACGG CCACACTTTC ACATTTGCGT CGAGCGAACT CTCCTATTGG ACGAGAAGGA 
AAACTTGCCA AGCCTCGTCA GCTGCATAAT ACTCAATGGG GAATGGTGTG TCCTGCTGAA 
ACTCCTGAAG GACAGGCTGT AGGTTTGGTG AAGAATCTGG CACTTATGGC ATACATCTCT 
GTCGGTTCGT TACCAGAGCC AATTCTGGAA TTCTTGGAAG AATGGTCAAT GGAAAATCTT 
GAAGAAGTGT CTCCATCGGC TATTGCCGAT GCTACGAAGA TCTTTGTCAA CGGTGCATGG 
GTGGGAATTC ATCGTGAACC CGATCAATTG ATGACGACGT TGAAGAAGCT TCGACGTCAA 
ATGGACATCA TCGTTTCCGA AGTATCAATG GTTCGTGATA TTCGTGACAG AGAAATTCGT 
ATCTACACTG ATGCTGGAAG AGTTTGCCGT CCATTACTTA TTGTTGAGAA TCAGAAATTG 
GCATTGAAGA AGAGACACAT TGATCAGTTG AAGGAGGCTG CTGACGAAGC GAATAAATAC 
ACATGGTCTG ATCTTGTTGG TGGTGGAGTT GTCGAACTGA TTGATTCAAT GGAAGAAGAA 
ACATCAATGA TTGCAATGAT GCCAGAAGAT TTGAGAAGTG GTGGTTACTG TGACACTCAT 
ACTCATTGTG AAATTCATCC AGCCATGATT CTCGGAGTTT GTGCCTCAAT CATTCCATTC 
CCAGATCATA ATCAATCACC ACGTAATACA TACCAAAGTG CTATGGGTAA GCAAGCTATG 
GGAGTTTATA CAACAAATTT CCATGTTCGA ATGGACACTC TTGCACACGT TCTCTACTAT 
CCACAAAAAC CGCTTGTCAC TACAAGATCT ATGGAATATC TTCGATTCAA TGAACTTCCA 
GCCGGAATCA ACGCAATTGT TGCTATTCTT TCATATTCCG GATATAATCA GGAAGATTCT 
GTCATTATGA ACAACTCTGC AATTGATCGA GGACTTTTCC GTTCTGTTTT CTATCGTTCT 
TATCGTGACA ATGAGGCAAA TCTTGACAAT GCAAATGAAG AACTGATTGA GAAACCAACT 
CGTGAAAAAT GTTCTGGAAT GCGTCATTCA TTGTACGATA AGCTTGATGA AGACGGAATC 
ATCTCTCCAG GAATGCGTGT GTCTGGCGAT GACGTCATTA TTGGAAAAAC AGTTGCTCTG 
CCAGATATTG ATGATGATTT GGATGCATCT GGGAAGAAAT ATCCAAAGAG AGATGCATCG 
ACATTCTTGA GAAGTTCTGA AACTGGTATT GTGGATCAGG TGATGCTCTC CCTCAACAGC 
GATGGAAATA AATTTGTCAA GATTCGTATG CGTTCTGTTC GTCTTCCACA AATTGGAGAT 
AAATTCGCAT CTCGACACGG TCAAAAAGGA ACCATGGGTA TTATGTATCG GCAAGAAGAT 
ATGCCATTTA CTGCTGAAGG ACTCACTCCT GATATCATCA TTAATCCTCA TGCCGTTCCA 
TCTCGTATGA CAATTGGTCA CTTGATCGAA TGCCTTCAGG GTAAACTATC AGCAAACAAG 
GGAGAAATCG GAGATGCTAC ACCATTCAAC GATACAGTCA ACGTACAAAA GATCTCTGGC 
CTACTATGTG AGTATGGTTA TCATTTGCGT GGTAACGAAG TGATGTACAA CGGACATACT 
GGAAAAAAGT TGACAACTCA AATCTTCTTT GGACCAACTT ATTATCAACG TCTCAAGCAT 
ATGGTCGATG ACAAGATTCA CTCACGTGCA CGCGGTCCAA TTCAGATGAT GAATCGACAG 
CCTATGGAAG GAAGAGCTCG TGACGGAGGT CTCCGTTTCG GAGAAATGGA ACGAGATTGT 
CAAATTTCCC ACGGAGCTAC TCAATTTTTG CGAGAACGTC TTTTTGAGGT GTCAGATCCA 
TATCATGTGT ACGTGTGCAA CAACTGTGGT CTTATTGTCG TCGCCAACTT GAGAACGAAC 
AGTTTCGAGT GCAAGGCTTG TCGCAACAAG ACCCAAGTAT CCGCTGTTCG TATTCCATAC 
GCTTGTAAGC TGCTCTTCCA GGAGTTAATG TCAATGTCTA TTGCTCCAAG ACTCATGGTC 
AAGCCTCGAC AATCAAAACG TTCAAAACAT CAATCGGAAG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498047.2
CDS	36..3620
Translation	MMYDDEDEMVNDPMDGDYIDDSDEISAEAWQEACWVVISAYFDEKGLVRQQLDSFDEFVQMNVQRIVEDSPPVELQSENQHLGTDMENPAKFSLKFNQIYLSKPTHWEKDGAPMPMMPNEARLRNLTYASPLYVDITKVVTRDDSATEKVYDKVFVGKVPVMLRSSYCMLSNMTDRDLTELNECPLDPGGYFVINGSEKVLIAQEKMATNTVYVFSMKDGKYAFKTECRSCLENSSRPTSTMWVNMLARGGGGGKKTAMGQRIIGILPYIKQEIPIMIVFRALGFVSDRDILGHIIYDFNDPEMMEMVKPSLDEAFVIQEQNVALNFIGARGAKPGVTREQRIKYAREILQKELLPHVGVSEHCETKKAFFIGYMVHRLLLAALGRRELDDRDHIGNKRLDLAGPLLAFLFRSLFRNLLKEMRMTAQKYINKNDDFALDVCVKTSTITRGLTYSLATGNWGDQKKAHQSRAGVSQVLNRLTYTATLSHLRRANSPIGREGKLAKPRQLHNTQWGMVCPAETPEGQAVGLVKNLALMAYISVGSLPEPILEFLEEWSMENLEEVSPSAIADATKIFVNGAWVGIHREPDQLMTTLKKLRRQMDIIVSEVSMVRDIRDREIRIYTDAGRVCRPLLIVENQKLALKKRHIDQLKEAADEANKYTWSDLVGGGVVELIDSMEEETSMIAMMPEDLRSGGYCDTHTHCEIHPAMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYTTNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFNELPAGINAIVAILSYSGYNQEDSVIMNNSAIDRGLFRSVFYRSYRDNEANLDNANEELIEKPTREKCSGMRHSLYDKLDEDGIISPGMRVSGDDVIIGKTVALPDIDDDLDASGKKYPKRDASTFLRSSETGIVDQVMLSLNSDGNKFVKIRMRSVRLPQIGDKFASRHGQKGTMGIMYRQEDMPFTAEGLTPDIIINPHAVPSRMTIGHLIECLQGKLSANKGEIGDATPFNDTVNVQKISGLLCEYGYHLRGNEVMYNGHTGKKLTTQIFFGPTYYQRLKHMVDDKIHSRARGPIQMMNRQPMEGRARDGGLRFGEMERDCQISHGATQFLRERLFEVSDPYHVYVCNNCGLIVVANLRTNSFECKACRNKTQVSAVRIPYACKLLFQELMSMSIAPRLMVKPRQSKRSKHQSEA

Target ORF information:

RefSeq Version	NM_065646.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB2 (rpb-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065646.6

ORF Insert Sequence:

ATGATGTACG ATGATGAGGA TGAGATGGTT AATGACCCGA TGGACGGAGA CTATATCGAT 
GATTCGGATG AAATCAGTGC AGAAGCATGG CAAGAAGCAT GTTGGGTTGT TATCAGTGCT 
TATTTCGATG AAAAGGGTCT CGTTCGTCAA CAACTTGATT CCTTCGATGA ATTCGTCCAA 
ATGAATGTGC AGCGAATTGT TGAGGATTCT CCGCCAGTCG AACTTCAATC AGAAAATCAG 
CATCTTGGCA CAGATATGGA AAATCCTGCG AAATTCTCAT TGAAATTCAA TCAAATTTAC 
CTCTCGAAAC CAACTCACTG GGAGAAAGAC GGAGCACCAA TGCCAATGAT GCCTAACGAA 
GCTCGTCTCC GAAATTTGAC ATACGCCTCG CCACTTTACG TAGATATCAC TAAAGTGGTG 
ACAAGAGACG ATTCAGCAAC GGAAAAAGTG TACGACAAAG TGTTCGTTGG AAAAGTTCCA 
GTTATGCTAC GGTCTAGTTA CTGTATGCTT TCCAATATGA CAGATCGTGA TCTAACAGAA 
CTCAATGAGT GCCCACTTGA TCCTGGAGGT TATTTTGTTA TCAATGGTTC GGAGAAAGTA 
CTTATTGCTC AAGAAAAGAT GGCGACTAAT ACAGTTTACG TGTTCTCAAT GAAAGACGGA 
AAATATGCAT TCAAAACGGA ATGTCGCTCT TGTTTGGAAA ATTCATCTCG TCCGACATCC 
ACAATGTGGG TTAATATGCT TGCAAGAGGA GGAGGTGGTG GAAAGAAGAC GGCGATGGGT 
CAACGAATTA TTGGAATTTT ACCATATATT AAACAAGAAA TTCCGATTAT GATTGTGTTC 
CGAGCGCTGG GATTCGTATC AGATCGTGAT ATTCTTGGAC ACATTATCTA CGATTTCAAT 
GATCCAGAAA TGATGGAAAT GGTCAAACCT TCGTTAGATG AAGCTTTTGT CATTCAGGAG 
CAAAATGTTG CTCTCAACTT TATCGGAGCT CGTGGAGCAA AACCAGGAGT AACCAGAGAA 
CAAAGAATCA AATATGCTCG TGAGATTCTT CAAAAAGAAC TTTTGCCTCA TGTTGGTGTA 
TCTGAGCATT GCGAAACGAA AAAAGCATTC TTCATCGGAT ACATGGTGCA TCGTCTTCTT 
CTAGCCGCCC TCGGACGTCG TGAACTTGAT GATCGTGATC ACATTGGAAA CAAAAGACTG 
GATTTAGCCG GACCACTTCT TGCTTTCTTG TTCCGTTCCC TTTTCAGAAA TCTTCTCAAG 
GAGATGAGAA TGACAGCTCA AAAGTATATC AACAAGAATG ACGACTTTGC TCTTGATGTC 
TGCGTCAAAA CTTCAACAAT AACTCGTGGA TTGACATATT CATTGGCTAC CGGTAATTGG 
GGTGATCAAA AGAAAGCACA TCAATCGAGA GCCGGTGTGT CTCAAGTACT CAATCGTCTC 
ACCTACACGG CCACACTTTC ACATTTGCGT CGAGCGAACT CTCCTATTGG ACGAGAAGGA 
AAACTTGCCA AGCCTCGTCA GCTGCATAAT ACTCAATGGG GAATGGTGTG TCCTGCTGAA 
ACTCCTGAAG GACAGGCTGT AGGTTTGGTG AAGAATCTGG CACTTATGGC ATACATCTCT 
GTCGGTTCGT TACCAGAGCC AATTCTGGAA TTCTTGGAAG AATGGTCAAT GGAAAATCTT 
GAAGAAGTGT CTCCATCGGC TATTGCCGAT GCTACGAAGA TCTTTGTCAA CGGTGCATGG 
GTGGGAATTC ATCGTGAACC CGATCAATTG ATGACGACGT TGAAGAAGCT TCGACGTCAA 
ATGGACATCA TCGTTTCCGA AGTATCAATG GTTCGTGATA TTCGTGACAG AGAAATTCGT 
ATCTACACTG ATGCTGGAAG AGTTTGCCGT CCATTACTTA TTGTTGAGAA TCAGAAATTG 
GCATTGAAGA AGAGACACAT TGATCAGTTG AAGGAGGCTG CTGACGAAGC GAATAAATAC 
ACATGGTCTG ATCTTGTTGG TGGTGGAGTT GTCGAACTGA TTGATTCAAT GGAAGAAGAA 
ACATCAATGA TTGCAATGAT GCCAGAAGAT TTGAGAAGTG GTGGTTACTG TGACACTCAT 
ACTCATTGTG AAATTCATCC AGCCATGATT CTCGGAGTTT GTGCCTCAAT CATTCCATTC 
CCAGATCATA ATCAATCACC ACGTAATACA TACCAAAGTG CTATGGGTAA GCAAGCTATG 
GGAGTTTATA CAACAAATTT CCATGTTCGA ATGGACACTC TTGCACACGT TCTCTACTAT 
CCACAAAAAC CGCTTGTCAC TACAAGATCT ATGGAATATC TTCGATTCAA TGAACTTCCA 
GCCGGAATCA ACGCAATTGT TGCTATTCTT TCATATTCCG GATATAATCA GGAAGATTCT 
GTCATTATGA ACAACTCTGC AATTGATCGA GGACTTTTCC GTTCTGTTTT CTATCGTTCT 
TATCGTGACA ATGAGGCAAA TCTTGACAAT GCAAATGAAG AACTGATTGA GAAACCAACT 
CGTGAAAAAT GTTCTGGAAT GCGTCATTCA TTGTACGATA AGCTTGATGA AGACGGAATC 
ATCTCTCCAG GAATGCGTGT GTCTGGCGAT GACGTCATTA TTGGAAAAAC AGTTGCTCTG 
CCAGATATTG ATGATGATTT GGATGCATCT GGGAAGAAAT ATCCAAAGAG AGATGCATCG 
ACATTCTTGA GAAGTTCTGA AACTGGTATT GTGGATCAGG TGATGCTCTC CCTCAACAGC 
GATGGAAATA AATTTGTCAA GATTCGTATG CGTTCTGTTC GTCTTCCACA AATTGGAGAT 
AAATTCGCAT CTCGACACGG TCAAAAAGGA ACCATGGGTA TTATGTATCG GCAAGAAGAT 
ATGCCATTTA CTGCTGAAGG ACTCACTCCT GATATCATCA TTAATCCTCA TGCCGTTCCA 
TCTCGTATGA CAATTGGTCA CTTGATCGAA TGCCTTCAGG GTAAACTATC AGCAAACAAG 
GGAGAAATCG GAGATGCTAC ACCATTCAAC GATACAGTCA ACGTACAAAA GATCTCTGGC 
CTACTATGTG AGTATGGTTA TCATTTGCGT GGTAACGAAG TGATGTACAA CGGACATACT 
GGAAAAAAGT TGACAACTCA AATCTTCTTT GGACCAACTT ATTATCAACG TCTCAAGCAT 
ATGGTCGATG ACAAGATTCA CTCACGTGCA CGCGGTCCAA TTCAGATGAT GAATCGACAG 
CCTATGGAAG GAAGAGCTCG TGACGGAGGT CTCCGTTTCG GAGAAATGGA ACGAGATTGT 
CAAATTTCCC ACGGAGCTAC TCAATTTTTG CGAGAACGTC TTTTTGAGGT GTCAGATCCA 
TATCATGTGT ACGTGTGCAA CAACTGTGGT CTTATTGTCG TCGCCAACTT GAGAACGAAC 
AGTTTCGAGT GCAAGGCTTG TCGCAACAAG ACCCAAGTAT CCGCTGTTCG TATTCCATAC 
GCTTGTAAGC TGCTCTTCCA GGAGTTAATG TCAATGTCTA TTGCTCCAAG ACTCATGGTC 
AAGCCTCGAC AATCAAAACG TTCAAAACAT CAATCGGAAG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rpb-2 ( NM_065646.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498047.2 Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB2 (rpb-2), partial mRNA.

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CloneID	OCa698874
Clone ID Related Accession (Same CDS sequence)	NM_065646.6 , NM_065646.5
Accession Version	NM_065646.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	DNA-directed RNA polymerase II subunit RPB2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Feb 12, 2019 this sequence version replaced NM_065646.4. COMPLETENESS: incomplete on both ends.

ATGATGTACG ATGATGAGGA TGAGATGGTT AATGACCCGA TGGACGGAGA CTATATCGAT 
GATTCGGATG AAATCAGTGC AGAAGCATGG CAAGAAGCAT GTTGGGTTGT TATCAGTGCT 
TATTTCGATG AAAAGGGTCT CGTTCGTCAA CAACTTGATT CCTTCGATGA ATTCGTCCAA 
ATGAATGTGC AGCGAATTGT TGAGGATTCT CCGCCAGTCG AACTTCAATC AGAAAATCAG 
CATCTTGGCA CAGATATGGA AAATCCTGCG AAATTCTCAT TGAAATTCAA TCAAATTTAC 
CTCTCGAAAC CAACTCACTG GGAGAAAGAC GGAGCACCAA TGCCAATGAT GCCTAACGAA 
GCTCGTCTCC GAAATTTGAC ATACGCCTCG CCACTTTACG TAGATATCAC TAAAGTGGTG 
ACAAGAGACG ATTCAGCAAC GGAAAAAGTG TACGACAAAG TGTTCGTTGG AAAAGTTCCA 
GTTATGCTAC GGTCTAGTTA CTGTATGCTT TCCAATATGA CAGATCGTGA TCTAACAGAA 
CTCAATGAGT GCCCACTTGA TCCTGGAGGT TATTTTGTTA TCAATGGTTC GGAGAAAGTA 
CTTATTGCTC AAGAAAAGAT GGCGACTAAT ACAGTTTACG TGTTCTCAAT GAAAGACGGA 
AAATATGCAT TCAAAACGGA ATGTCGCTCT TGTTTGGAAA ATTCATCTCG TCCGACATCC 
ACAATGTGGG TTAATATGCT TGCAAGAGGA GGAGGTGGTG GAAAGAAGAC GGCGATGGGT 
CAACGAATTA TTGGAATTTT ACCATATATT AAACAAGAAA TTCCGATTAT GATTGTGTTC 
CGAGCGCTGG GATTCGTATC AGATCGTGAT ATTCTTGGAC ACATTATCTA CGATTTCAAT 
GATCCAGAAA TGATGGAAAT GGTCAAACCT TCGTTAGATG AAGCTTTTGT CATTCAGGAG 
CAAAATGTTG CTCTCAACTT TATCGGAGCT CGTGGAGCAA AACCAGGAGT AACCAGAGAA 
CAAAGAATCA AATATGCTCG TGAGATTCTT CAAAAAGAAC TTTTGCCTCA TGTTGGTGTA 
TCTGAGCATT GCGAAACGAA AAAAGCATTC TTCATCGGAT ACATGGTGCA TCGTCTTCTT 
CTAGCCGCCC TCGGACGTCG TGAACTTGAT GATCGTGATC ACATTGGAAA CAAAAGACTG 
GATTTAGCCG GACCACTTCT TGCTTTCTTG TTCCGTTCCC TTTTCAGAAA TCTTCTCAAG 
GAGATGAGAA TGACAGCTCA AAAGTATATC AACAAGAATG ACGACTTTGC TCTTGATGTC 
TGCGTCAAAA CTTCAACAAT AACTCGTGGA TTGACATATT CATTGGCTAC CGGTAATTGG 
GGTGATCAAA AGAAAGCACA TCAATCGAGA GCCGGTGTGT CTCAAGTACT CAATCGTCTC 
ACCTACACGG CCACACTTTC ACATTTGCGT CGAGCGAACT CTCCTATTGG ACGAGAAGGA 
AAACTTGCCA AGCCTCGTCA GCTGCATAAT ACTCAATGGG GAATGGTGTG TCCTGCTGAA 
ACTCCTGAAG GACAGGCTGT AGGTTTGGTG AAGAATCTGG CACTTATGGC ATACATCTCT 
GTCGGTTCGT TACCAGAGCC AATTCTGGAA TTCTTGGAAG AATGGTCAAT GGAAAATCTT 
GAAGAAGTGT CTCCATCGGC TATTGCCGAT GCTACGAAGA TCTTTGTCAA CGGTGCATGG 
GTGGGAATTC ATCGTGAACC CGATCAATTG ATGACGACGT TGAAGAAGCT TCGACGTCAA 
ATGGACATCA TCGTTTCCGA AGTATCAATG GTTCGTGATA TTCGTGACAG AGAAATTCGT 
ATCTACACTG ATGCTGGAAG AGTTTGCCGT CCATTACTTA TTGTTGAGAA TCAGAAATTG 
GCATTGAAGA AGAGACACAT TGATCAGTTG AAGGAGGCTG CTGACGAAGC GAATAAATAC 
ACATGGTCTG ATCTTGTTGG TGGTGGAGTT GTCGAACTGA TTGATTCAAT GGAAGAAGAA 
ACATCAATGA TTGCAATGAT GCCAGAAGAT TTGAGAAGTG GTGGTTACTG TGACACTCAT 
ACTCATTGTG AAATTCATCC AGCCATGATT CTCGGAGTTT GTGCCTCAAT CATTCCATTC 
CCAGATCATA ATCAATCACC ACGTAATACA TACCAAAGTG CTATGGGTAA GCAAGCTATG 
GGAGTTTATA CAACAAATTT CCATGTTCGA ATGGACACTC TTGCACACGT TCTCTACTAT 
CCACAAAAAC CGCTTGTCAC TACAAGATCT ATGGAATATC TTCGATTCAA TGAACTTCCA 
GCCGGAATCA ACGCAATTGT TGCTATTCTT TCATATTCCG GATATAATCA GGAAGATTCT 
GTCATTATGA ACAACTCTGC AATTGATCGA GGACTTTTCC GTTCTGTTTT CTATCGTTCT 
TATCGTGACA ATGAGGCAAA TCTTGACAAT GCAAATGAAG AACTGATTGA GAAACCAACT 
CGTGAAAAAT GTTCTGGAAT GCGTCATTCA TTGTACGATA AGCTTGATGA AGACGGAATC 
ATCTCTCCAG GAATGCGTGT GTCTGGCGAT GACGTCATTA TTGGAAAAAC AGTTGCTCTG 
CCAGATATTG ATGATGATTT GGATGCATCT GGGAAGAAAT ATCCAAAGAG AGATGCATCG 
ACATTCTTGA GAAGTTCTGA AACTGGTATT GTGGATCAGG TGATGCTCTC CCTCAACAGC 
GATGGAAATA AATTTGTCAA GATTCGTATG CGTTCTGTTC GTCTTCCACA AATTGGAGAT 
AAATTCGCAT CTCGACACGG TCAAAAAGGA ACCATGGGTA TTATGTATCG GCAAGAAGAT 
ATGCCATTTA CTGCTGAAGG ACTCACTCCT GATATCATCA TTAATCCTCA TGCCGTTCCA 
TCTCGTATGA CAATTGGTCA CTTGATCGAA TGCCTTCAGG GTAAACTATC AGCAAACAAG 
GGAGAAATCG GAGATGCTAC ACCATTCAAC GATACAGTCA ACGTACAAAA GATCTCTGGC 
CTACTATGTG AGTATGGTTA TCATTTGCGT GGTAACGAAG TGATGTACAA CGGACATACT 
GGAAAAAAGT TGACAACTCA AATCTTCTTT GGACCAACTT ATTATCAACG TCTCAAGCAT 
ATGGTCGATG ACAAGATTCA CTCACGTGCA CGCGGTCCAA TTCAGATGAT GAATCGACAG 
CCTATGGAAG GAAGAGCTCG TGACGGAGGT CTCCGTTTCG GAGAAATGGA ACGAGATTGT 
CAAATTTCCC ACGGAGCTAC TCAATTTTTG CGAGAACGTC TTTTTGAGGT GTCAGATCCA 
TATCATGTGT ACGTGTGCAA CAACTGTGGT CTTATTGTCG TCGCCAACTT GAGAACGAAC 
AGTTTCGAGT GCAAGGCTTG TCGCAACAAG ACCCAAGTAT CCGCTGTTCG TATTCCATAC 
GCTTGTAAGC TGCTCTTCCA GGAGTTAATG TCAATGTCTA TTGCTCCAAG ACTCATGGTC 
AAGCCTCGAC AATCAAAACG TTCAAAACAT CAATCGGAAG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498047.2
CDS	1..3585
Translation	MMYDDEDEMVNDPMDGDYIDDSDEISAEAWQEACWVVISAYFDEKGLVRQQLDSFDEFVQMNVQRIVEDSPPVELQSENQHLGTDMENPAKFSLKFNQIYLSKPTHWEKDGAPMPMMPNEARLRNLTYASPLYVDITKVVTRDDSATEKVYDKVFVGKVPVMLRSSYCMLSNMTDRDLTELNECPLDPGGYFVINGSEKVLIAQEKMATNTVYVFSMKDGKYAFKTECRSCLENSSRPTSTMWVNMLARGGGGGKKTAMGQRIIGILPYIKQEIPIMIVFRALGFVSDRDILGHIIYDFNDPEMMEMVKPSLDEAFVIQEQNVALNFIGARGAKPGVTREQRIKYAREILQKELLPHVGVSEHCETKKAFFIGYMVHRLLLAALGRRELDDRDHIGNKRLDLAGPLLAFLFRSLFRNLLKEMRMTAQKYINKNDDFALDVCVKTSTITRGLTYSLATGNWGDQKKAHQSRAGVSQVLNRLTYTATLSHLRRANSPIGREGKLAKPRQLHNTQWGMVCPAETPEGQAVGLVKNLALMAYISVGSLPEPILEFLEEWSMENLEEVSPSAIADATKIFVNGAWVGIHREPDQLMTTLKKLRRQMDIIVSEVSMVRDIRDREIRIYTDAGRVCRPLLIVENQKLALKKRHIDQLKEAADEANKYTWSDLVGGGVVELIDSMEEETSMIAMMPEDLRSGGYCDTHTHCEIHPAMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYTTNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFNELPAGINAIVAILSYSGYNQEDSVIMNNSAIDRGLFRSVFYRSYRDNEANLDNANEELIEKPTREKCSGMRHSLYDKLDEDGIISPGMRVSGDDVIIGKTVALPDIDDDLDASGKKYPKRDASTFLRSSETGIVDQVMLSLNSDGNKFVKIRMRSVRLPQIGDKFASRHGQKGTMGIMYRQEDMPFTAEGLTPDIIINPHAVPSRMTIGHLIECLQGKLSANKGEIGDATPFNDTVNVQKISGLLCEYGYHLRGNEVMYNGHTGKKLTTQIFFGPTYYQRLKHMVDDKIHSRARGPIQMMNRQPMEGRARDGGLRFGEMERDCQISHGATQFLRERLFEVSDPYHVYVCNNCGLIVVANLRTNSFECKACRNKTQVSAVRIPYACKLLFQELMSMSIAPRLMVKPRQSKRSKHQSEA

Target ORF information:

RefSeq Version	NM_065646.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB2 (rpb-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065646.5

ORF Insert Sequence:

ATGATGTACG ATGATGAGGA TGAGATGGTT AATGACCCGA TGGACGGAGA CTATATCGAT 
GATTCGGATG AAATCAGTGC AGAAGCATGG CAAGAAGCAT GTTGGGTTGT TATCAGTGCT 
TATTTCGATG AAAAGGGTCT CGTTCGTCAA CAACTTGATT CCTTCGATGA ATTCGTCCAA 
ATGAATGTGC AGCGAATTGT TGAGGATTCT CCGCCAGTCG AACTTCAATC AGAAAATCAG 
CATCTTGGCA CAGATATGGA AAATCCTGCG AAATTCTCAT TGAAATTCAA TCAAATTTAC 
CTCTCGAAAC CAACTCACTG GGAGAAAGAC GGAGCACCAA TGCCAATGAT GCCTAACGAA 
GCTCGTCTCC GAAATTTGAC ATACGCCTCG CCACTTTACG TAGATATCAC TAAAGTGGTG 
ACAAGAGACG ATTCAGCAAC GGAAAAAGTG TACGACAAAG TGTTCGTTGG AAAAGTTCCA 
GTTATGCTAC GGTCTAGTTA CTGTATGCTT TCCAATATGA CAGATCGTGA TCTAACAGAA 
CTCAATGAGT GCCCACTTGA TCCTGGAGGT TATTTTGTTA TCAATGGTTC GGAGAAAGTA 
CTTATTGCTC AAGAAAAGAT GGCGACTAAT ACAGTTTACG TGTTCTCAAT GAAAGACGGA 
AAATATGCAT TCAAAACGGA ATGTCGCTCT TGTTTGGAAA ATTCATCTCG TCCGACATCC 
ACAATGTGGG TTAATATGCT TGCAAGAGGA GGAGGTGGTG GAAAGAAGAC GGCGATGGGT 
CAACGAATTA TTGGAATTTT ACCATATATT AAACAAGAAA TTCCGATTAT GATTGTGTTC 
CGAGCGCTGG GATTCGTATC AGATCGTGAT ATTCTTGGAC ACATTATCTA CGATTTCAAT 
GATCCAGAAA TGATGGAAAT GGTCAAACCT TCGTTAGATG AAGCTTTTGT CATTCAGGAG 
CAAAATGTTG CTCTCAACTT TATCGGAGCT CGTGGAGCAA AACCAGGAGT AACCAGAGAA 
CAAAGAATCA AATATGCTCG TGAGATTCTT CAAAAAGAAC TTTTGCCTCA TGTTGGTGTA 
TCTGAGCATT GCGAAACGAA AAAAGCATTC TTCATCGGAT ACATGGTGCA TCGTCTTCTT 
CTAGCCGCCC TCGGACGTCG TGAACTTGAT GATCGTGATC ACATTGGAAA CAAAAGACTG 
GATTTAGCCG GACCACTTCT TGCTTTCTTG TTCCGTTCCC TTTTCAGAAA TCTTCTCAAG 
GAGATGAGAA TGACAGCTCA AAAGTATATC AACAAGAATG ACGACTTTGC TCTTGATGTC 
TGCGTCAAAA CTTCAACAAT AACTCGTGGA TTGACATATT CATTGGCTAC CGGTAATTGG 
GGTGATCAAA AGAAAGCACA TCAATCGAGA GCCGGTGTGT CTCAAGTACT CAATCGTCTC 
ACCTACACGG CCACACTTTC ACATTTGCGT CGAGCGAACT CTCCTATTGG ACGAGAAGGA 
AAACTTGCCA AGCCTCGTCA GCTGCATAAT ACTCAATGGG GAATGGTGTG TCCTGCTGAA 
ACTCCTGAAG GACAGGCTGT AGGTTTGGTG AAGAATCTGG CACTTATGGC ATACATCTCT 
GTCGGTTCGT TACCAGAGCC AATTCTGGAA TTCTTGGAAG AATGGTCAAT GGAAAATCTT 
GAAGAAGTGT CTCCATCGGC TATTGCCGAT GCTACGAAGA TCTTTGTCAA CGGTGCATGG 
GTGGGAATTC ATCGTGAACC CGATCAATTG ATGACGACGT TGAAGAAGCT TCGACGTCAA 
ATGGACATCA TCGTTTCCGA AGTATCAATG GTTCGTGATA TTCGTGACAG AGAAATTCGT 
ATCTACACTG ATGCTGGAAG AGTTTGCCGT CCATTACTTA TTGTTGAGAA TCAGAAATTG 
GCATTGAAGA AGAGACACAT TGATCAGTTG AAGGAGGCTG CTGACGAAGC GAATAAATAC 
ACATGGTCTG ATCTTGTTGG TGGTGGAGTT GTCGAACTGA TTGATTCAAT GGAAGAAGAA 
ACATCAATGA TTGCAATGAT GCCAGAAGAT TTGAGAAGTG GTGGTTACTG TGACACTCAT 
ACTCATTGTG AAATTCATCC AGCCATGATT CTCGGAGTTT GTGCCTCAAT CATTCCATTC 
CCAGATCATA ATCAATCACC ACGTAATACA TACCAAAGTG CTATGGGTAA GCAAGCTATG 
GGAGTTTATA CAACAAATTT CCATGTTCGA ATGGACACTC TTGCACACGT TCTCTACTAT 
CCACAAAAAC CGCTTGTCAC TACAAGATCT ATGGAATATC TTCGATTCAA TGAACTTCCA 
GCCGGAATCA ACGCAATTGT TGCTATTCTT TCATATTCCG GATATAATCA GGAAGATTCT 
GTCATTATGA ACAACTCTGC AATTGATCGA GGACTTTTCC GTTCTGTTTT CTATCGTTCT 
TATCGTGACA ATGAGGCAAA TCTTGACAAT GCAAATGAAG AACTGATTGA GAAACCAACT 
CGTGAAAAAT GTTCTGGAAT GCGTCATTCA TTGTACGATA AGCTTGATGA AGACGGAATC 
ATCTCTCCAG GAATGCGTGT GTCTGGCGAT GACGTCATTA TTGGAAAAAC AGTTGCTCTG 
CCAGATATTG ATGATGATTT GGATGCATCT GGGAAGAAAT ATCCAAAGAG AGATGCATCG 
ACATTCTTGA GAAGTTCTGA AACTGGTATT GTGGATCAGG TGATGCTCTC CCTCAACAGC 
GATGGAAATA AATTTGTCAA GATTCGTATG CGTTCTGTTC GTCTTCCACA AATTGGAGAT 
AAATTCGCAT CTCGACACGG TCAAAAAGGA ACCATGGGTA TTATGTATCG GCAAGAAGAT 
ATGCCATTTA CTGCTGAAGG ACTCACTCCT GATATCATCA TTAATCCTCA TGCCGTTCCA 
TCTCGTATGA CAATTGGTCA CTTGATCGAA TGCCTTCAGG GTAAACTATC AGCAAACAAG 
GGAGAAATCG GAGATGCTAC ACCATTCAAC GATACAGTCA ACGTACAAAA GATCTCTGGC 
CTACTATGTG AGTATGGTTA TCATTTGCGT GGTAACGAAG TGATGTACAA CGGACATACT 
GGAAAAAAGT TGACAACTCA AATCTTCTTT GGACCAACTT ATTATCAACG TCTCAAGCAT 
ATGGTCGATG ACAAGATTCA CTCACGTGCA CGCGGTCCAA TTCAGATGAT GAATCGACAG 
CCTATGGAAG GAAGAGCTCG TGACGGAGGT CTCCGTTTCG GAGAAATGGA ACGAGATTGT 
CAAATTTCCC ACGGAGCTAC TCAATTTTTG CGAGAACGTC TTTTTGAGGT GTCAGATCCA 
TATCATGTGT ACGTGTGCAA CAACTGTGGT CTTATTGTCG TCGCCAACTT GAGAACGAAC 
AGTTTCGAGT GCAAGGCTTG TCGCAACAAG ACCCAAGTAT CCGCTGTTCG TATTCCATAC 
GCTTGTAAGC TGCTCTTCCA GGAGTTAATG TCAATGTCTA TTGCTCCAAG ACTCATGGTC 
AAGCCTCGAC AATCAAAACG TTCAAAACAT CAATCGGAAG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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rpb-2 cDNA ORF clone, Caenorhabditis elegans

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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