POLR2B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR2B
Entrez Gene ID	5431
Full Name	RNA polymerase II subunit B
Synonyms	POL2RB,RPB2,hRPB140
General protein information	Preferred Names RNA polymerase II subunit B Names DNA-directed RNA polymerase II subunit RPB2 DNA-directed RNA polymerase II 140 kDa polypeptide DNA-directed RNA polymerase II subunit B RNA polymerase II second largest subunit RNA polymerase II subunit 2 RNA polymerase II subunit B2 polymerase (RNA) II (DNA directed) polypeptide B, 140kDa polymerase (RNA) II subunit B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q12
Summary	This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014].
Disorder MIM:	180661

mRNA and Protein(s)

mRNA	Protein	Name
NM_001303268.1	NP_001290197.1	DNA-directed RNA polymerase II subunit RPB2 isoform 2
NM_000938.2	NP_000929.1	DNA-directed RNA polymerase II subunit RPB2 isoform 1
NM_000938.3	NP_000929.1	DNA-directed RNA polymerase II subunit RPB2 isoform 1
NM_001303269.1	NP_001290198.1	DNA-directed RNA polymerase II subunit RPB2 isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLR2B	NP_000929.1
Rattus norvegicus (Norway rat)	Polr2b	NP_001099472.1
Gallus gallus (chicken)	POLR2B	NP_001006448.1
Drosophila melanogaster (fruit fly)	RpII140	NP_476706.1
Mus musculus (house mouse)	Polr2b	NP_722493.2
Danio rerio (zebrafish)	polr2b	NP_001019632.2
Schizosaccharomyces pombe (fission yeast)	rpb2	NP_593101.2
Magnaporthe oryzae (rice blast fungus)	MGG_04714	XP_003710821.1
Neurospora crassa	NCU05120	XP_957106.2
Arabidopsis thaliana (thale cress)	NRPB2	NP_193902.1
Canis lupus familiaris (dog)	POLR2B	XP_532382.3
Saccharomyces cerevisiae (baker's yeast)	RPB2	NP_014794.3
Kluyveromyces lactis	KLLA0B05577g	XP_451784.1
Eremothecium gossypii	AGOS_AFR404C	NP_985951.1
	POLR2B	XP_001083325.2
Pan troglodytes (chimpanzee)	POLR2B	XP_517275.2
Bos taurus (cattle)	POLR2B	NP_001092552.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003648	XP_313416.4
Caenorhabditis elegans (roundworm)	rpb-2	NP_498047.1
Oryza sativa (rice)	Os03g0646800	NP_001050770.1
Xenopus tropicalis (tropical clawed frog)	polr2b	XP_002937598.2

Gene Ontology
Bibliography

Related articles in PubMed

Meta-analysis of genome-wide association studies of adult height in East Asians identifies 17 novel loci.
He M, Xu M, Zhang B, Liang J, Chen P, Lee JY, Johnson TA, Li H, Yang X, Dai J, Liang L, Gui L, Qi Q, Huang J, Li Y, Adair LS, Aung T, Cai Q, Cheng CY, Cho MC, Cho YS, Chu M, Cui B, Gao YT, Go MJ, Gu D, Gu W, Guo H, Hao Y, Hong J, Hu Z, Hu Y, Huang J, Hwang JY, Ikram MK, Jin G, Kang DH, Khor CC, Kim BJ, Kim HT, Kubo M, Lee J, Lee J, Lee NR, Li R, Li J, Liu J, Longe J, Lu W, Lu X, Miao X, Okada Y, Ong RT, Qiu G, Seielstad M, Sim X, Song H, Takeuchi F, Tanaka T, Taylor PR, Wang L, Wang W, Wang Y, Wu C, Wu Y, Xiang YB, Yamamoto K, Yang H, Liao M, Yokota M, Young T, Zhang X, Kato N, Wang QK, Zheng W, Hu FB, Lin D, Shen H, Teo YY, Mo Z, Wong TY, Lin X, Mohlke KL, Ning G, Tsunoda T, Han BG, Shu XO, Tai ES, Wu T, Qi L
Human molecular genetics24(6)1791-800(2015 Mar)

A quantitative telomeric chromatin isolation protocol identifies different telomeric states.
Grolimund L, Aeby E, Hamelin R, Armand F, Chiappe D, Moniatte M, Lingner J
Nature communications42848(2013)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Genome-wide association study identifies two susceptibility loci for exudative age-related macular degeneration in the Japanese population.
Arakawa S, Takahashi A, Ashikawa K, Hosono N, Aoi T, Yasuda M, Oshima Y, Yoshida S, Enaida H, Tsuchihashi T, Mori K, Honda S, Negi A, Arakawa A, Kadonosono K, Kiyohara Y, Kamatani N, Nakamura Y, Ishibashi T, Kubo M
Nature genetics43(10)1001-4(2011 Sep)

The RPB2 flap loop of human RNA polymerase II is dispensable for transcription initiation and elongation.
Palangat M, Grass JA, Langelier MF, Coulombe B, Landick R
Molecular and cellular biology31(16)3312-25(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate transcription of many genes, is regulated after the initiation step, by pausing of RNA polymerase II during elongation through the promoter-proximal region.
Title: Pol II waiting in the starting gates: Regulating the transition from transcription initiation into productive elongation.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study of gene-disease association, gene-gene interaction, and gene-environment interaction. (HuGE Navigator)
Title: Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk.

By reducing transcriptional fidelity in terms of not only discrimination of incoming nucleotides but also recognition of templates, HDAg may facilitate the unusual RNA-dependent RNA synthesis by Pol II.
Title: Hepatitis delta antigen binds to the clamp of RNA polymerase II and affects transcriptional fidelity.

the human Maf1 protein negatively regulates transcription by all three nuclear Pols, Pol I, Pol II and Pol III
Title: Mammalian Maf1 is a negative regulator of transcription by all three nuclear RNA polymerases.

The following POLR2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu59820	NM_000938.3 Latest version!	Homo sapiens RNA polymerase II subunit B (POLR2B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu59821	NM_001303268.1 Latest version!	Homo sapiens RNA polymerase II subunit B (POLR2B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu59822	NM_001303269.1 Latest version!	Homo sapiens RNA polymerase II subunit B (POLR2B), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu59820	NM_000938.2	Homo sapiens RNA polymerase II subunit B (POLR2B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR2B ( NM_000938.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000929.1 Homo sapiens RNA polymerase II subunit B (POLR2B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu59820
Clone ID Related Accession (Same CDS sequence)	NM_000938.2 , NM_000938.3
Accession Version	NM_000938.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase II subunit RPB2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC323029.1, BC023503.2, AK294901.1 and BU619780.1. On Dec 25, 2014 this sequence version replaced NM_000938.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.278458.1, BC023503.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACGACG CGGATGAGGA TATGCAATAT GATGAGGATG ATGATGAAAT CACCCCGGAT 
TTGTGGCAAG AAGCATGCTG GATTGTAATC AGTTCCTATT TTGACGAGAA AGGCTTGGTT 
AGACAACAGC TGGATTCTTT TGATGAGTTT ATTCAGATGT CTGTTCAAAG AATTGTGGAA 
GACGCTCCTC CTATAGACCT ACAGGCTGAA GCTCAGCATG CTAGTGGAGA AGTTGAAGAA 
CCGCCACGAT ATTTGCTGAA GTTTGAACAA ATTTATCTTT CCAAGCCTAC CCATTGGGAA 
AGAGATGGTG CTCCTTCACC AATGATGCCC AATGAAGCTA GATTAAGGAA TCTCACGTAT 
TCTGCTCCGC TTTATGTTGA TATAACAAAA ACAGTCATTA AAGAAGGTGA AGAACAACTT 
CAGACTCAGC ATCAGAAAAC TTTTATAGGA AAAATTCCAA TTATGTTGCG GTCAACTTAC 
TGCCTTTTGA ATGGCTTGAC AGATCGTGAT CTTTGTGAGT TAAATGAATG CCCTTTGGAT 
CCTGGTGGCT ATTTCATTAT TAATGGATCA GAAAAGGTTC TGATTGCCCA AGAGAAAATG 
GCAACAAACA CAGTTTATGT GTTTGCCAAA AAGGATTCTA AATATGCCTA CACAGGAGAG 
TGTAGATCAT GTCTTGAGAA TTCTTCCCGA CCCACCAGTA CTATATGGGT TAGCATGCTG 
GCAAGAGGAG GACAGGGTGC CAAGAAGAGT GCTATTGGTC AGCGCATTGT GGCAACTCTA 
CCATATATCA AGCAAGAAGT TCCCATCATT ATTGTGTTCA GAGCATTAGG TTTTGTGTCC 
GACAGAGATA TTTTAGAACA TATTATTTAT GATTTTGAAG ATCCAGAGAT GATGGAAATG 
GTTAAACCTT CTCTCGATGA AGCTTTTGTC ATCCAAGAAC AGAATGTTGC ACTAAATTTC 
ATTGGTTCAA GAGGAGCAAA GCCTGGTGTT ACTAAAGAGA AAAGAATTAA ATATGCAAAG 
GAAGTTTTAC AAAAAGAAAT GCTCCCTCAT GTTGGTGTCA GTGATTTTTG TGAGACCAAA 
AAAGCCTATT TCTTGGGATA CATGGTTCAT AGGTTACTTC TGGCAGCTTT GGGTAGAAGA 
GAACTAGATG ACAGAGATCA CTATGGAAAC AAGAGATTGG ATCTTGCTGG GCCGCTGCTT 
GCATTCTTAT TTAGAGGTAT GTTTAAGAAT TTGCTTAAAG AAGTGCGGAT CTATGCACAG 
AAATTTATTG ATCGAGGAAA GGATTTTAAC TTGGAGTTGG CAATTAAAAC ACGGATCATA 
TCTGATGGCC TAAAATACTC TTTAGCTACT GGAAACTGGG GTGATCAAAA GAAAGCTCAT 
CAAGCCAGAG CTGGAGTATC TCAGGTGTTA AACCGCCTGA CTTTTGCGTC TACTCTTTCT 
CACCTGCGTC GTTTAAATTC TCCTATTGGT AGAGACGGCA AGCTAGCAAA ACCAAGACAG 
TTGCATAATA CGTTGTGGGG AATGGTGTGT CCTGCCGAGA CCCCAGAGGG CCATGCTGTA 
GGACTTGTGA AGAATTTAGC CTTGATGGCG TATATTTCAG TTGGATCTCA ACCATCTCCA 
ATTCTGGAAT TTTTAGAAGA ATGGAGTATG GAAAATTTAG AAGAAATTTC TCCTGCAGCT 
ATTGCTGATG CAACCAAGAT TTTTGTTAAT GGCTGCTGGG TTGGAATACA TAAAGATCCC 
GAACAACTTA TGAACACCCT AAGGAAATTG AGACGTCAGA TGGACATCAT TGTGTCTGAA 
GTTTCTATGA TCAGAGATAT TCGAGAGAGG GAGATTCGGA TCTATACGGA TGCAGGCCGT 
ATTTGTAGAC CACTTCTGAT TGTGGAAAAA CAAAAGCTAC TTTTGAAGAA GAGGCATATT 
GACCAATTGA AAGAGAGAGA ATATAACAAC TATAGTTGGC AGGATCTTGT GGCCAGTGGG 
GTAGTGGAGT ATATTGATAC CCTGGAAGAA GAAACAGTGA TGCTTGCAAT GACTCCAGAT 
GATTTACAGG AGAAAGAAGT AGCTTATTGT TCCACATATA CACACTGTGA GATTCATCCC 
TCAATGATCC TTGGTGTCTG TGCATCTATT ATTCCCTTTC CTGATCATAA CCAGTCCCCT 
AGAAACACAT ACCAGTCTGC TATGGGTAAG CAGGCTATGG GAGTTTACAT CACCAACTTC 
CATGTTCGCA TGGACACATT GGCCCATGTT CTCTATTATC CTCAAAAGCC ACTTGTGACT 
ACACGGTCTA TGGAATATCT ACGATTTAGA GAGCTGCCAG CAGGCATCAA CTCAATTGTG 
GCCATTGCAT CATACACTGG ATATAATCAG GAAGACTCTG TTATCATGAA TCGTTCAGCT 
GTAGACCGCG GCTTCTTCAG GTCTGTTTTC TATCGCTCAT ACAAAGAACA GGAGTCTAAA 
AAAGGATTTG ATCAAGAAGA AGTTTTTGAG AAGCCTACAC GTGAAACATG CCAGGGCATG 
AGGCATGCCA TTTACGACAA GCTGGATGAT GATGGTTTGA TAGCTCCAGG GGTTCGTGTA 
TCAGGAGATG ATGTTATTAT AGGCAAAACA GTCACCTTGC CTGAAAATGA AGATGAATTG 
GAGAGCACCA ATAGACGCTA TACCAAGAGA GACTGTAGCA CTTTTCTCAG AACTAGCGAG 
ACGGGCATTG TGGATCAGGT TATGGTAACT CTCAATCAGG AAGGATATAA ATTTTGTAAA 
ATAAGGGTAC GCTCTGTTAG GATTCCACAG ATTGGAGACA AATTTGCTAG TCGACATGGT 
CAAAAGGGTA CTTGTGGTAT TCAGTATAGA CAAGAGGATA TGCCTTTCAC CTGTGAAGGT 
ATCACCCCTG ATATCATCAT CAATCCCCAT GCCATCCCCT CTCGTATGAC TATTGGTCAC 
TTAATTGAAT GCCTTCAAGG GAAGGTATCG GCTAACAAGG GTGAAATTGG TGATGCCACT 
CCATTTAATG ATGCTGTTAA CGTGCAGAAG ATTTCTAATC TTTTATCTGA TTATGGCTAT 
CATCTCAGAG GAAATGAGGT CCTGTACAAT GGGTTCACTG GTCGAAAAAT CACATCACAA 
ATATTTATTG GCCCCACTTA TTACCAGCGT TTGAAGCATA TGGTGGATGA TAAGATTCAC 
TCTCGTGCTA GGGGACCTAT TCAGATCCTC AATAGACAGC CCATGGAGGG TAGATCTCGT 
GATGGTGGCC TGCGTTTTGG AGAAATGGAA CGAGATTGTC AGATTGCCCA TGGAGCAGCC 
CAGTTTTTAA GGGAAAGATT GTTTGAGGCA TCAGATCCAT ATCAGGTTCA TGTTTGCAAT 
CTTTGTGGAA TAATGGCGAT TGCCAACACC AGGACCCATA CATATGAATG CAGGGGCTGC 
CGCAATAAAA CCCAGATTTC TTTGGTGCGA ATGCCTTACG CATGCAAACT ATTGTTTCAG 
GAACTTATGT CTATGAGTAT TGCACCGCGA ATGATGAGTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000929.1
CDS	347..3871
Translation	MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

Target ORF information:

RefSeq Version	NM_000938.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit B (POLR2B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000938.2

ORF Insert Sequence:

ATGTACGACG CGGATGAGGA TATGCAATAT GATGAGGATG ATGATGAAAT CACCCCGGAT 
TTGTGGCAAG AAGCATGCTG GATTGTAATC AGTTCCTATT TTGACGAGAA AGGCTTGGTT 
AGACAACAGC TGGATTCTTT TGATGAGTTT ATTCAGATGT CTGTTCAAAG AATTGTGGAA 
GACGCTCCTC CTATAGACCT ACAGGCTGAA GCTCAGCATG CTAGTGGAGA AGTTGAAGAA 
CCGCCACGAT ATTTGCTGAA GTTTGAACAA ATTTATCTTT CCAAGCCTAC CCATTGGGAA 
AGAGATGGTG CTCCTTCACC AATGATGCCC AATGAAGCTA GATTAAGGAA TCTCACGTAT 
TCTGCTCCGC TTTATGTTGA TATAACAAAA ACAGTCATTA AAGAAGGTGA AGAACAACTT 
CAGACTCAGC ATCAGAAAAC TTTTATAGGA AAAATTCCAA TTATGTTGCG GTCAACTTAC 
TGCCTTTTGA ATGGCTTGAC AGATCGTGAT CTTTGTGAGT TAAATGAATG CCCTTTGGAT 
CCTGGTGGCT ATTTCATTAT TAATGGATCA GAAAAGGTTC TGATTGCCCA AGAGAAAATG 
GCAACAAACA CAGTTTATGT GTTTGCCAAA AAGGATTCTA AATATGCCTA CACAGGAGAG 
TGTAGATCAT GTCTTGAGAA TTCTTCCCGA CCCACCAGTA CTATATGGGT TAGCATGCTG 
GCAAGAGGAG GACAGGGTGC CAAGAAGAGT GCTATTGGTC AGCGCATTGT GGCAACTCTA 
CCATATATCA AGCAAGAAGT TCCCATCATT ATTGTGTTCA GAGCATTAGG TTTTGTGTCC 
GACAGAGATA TTTTAGAACA TATTATTTAT GATTTTGAAG ATCCAGAGAT GATGGAAATG 
GTTAAACCTT CTCTCGATGA AGCTTTTGTC ATCCAAGAAC AGAATGTTGC ACTAAATTTC 
ATTGGTTCAA GAGGAGCAAA GCCTGGTGTT ACTAAAGAGA AAAGAATTAA ATATGCAAAG 
GAAGTTTTAC AAAAAGAAAT GCTCCCTCAT GTTGGTGTCA GTGATTTTTG TGAGACCAAA 
AAAGCCTATT TCTTGGGATA CATGGTTCAT AGGTTACTTC TGGCAGCTTT GGGTAGAAGA 
GAACTAGATG ACAGAGATCA CTATGGAAAC AAGAGATTGG ATCTTGCTGG GCCGCTGCTT 
GCATTCTTAT TTAGAGGTAT GTTTAAGAAT TTGCTTAAAG AAGTGCGGAT CTATGCACAG 
AAATTTATTG ATCGAGGAAA GGATTTTAAC TTGGAGTTGG CAATTAAAAC ACGGATCATA 
TCTGATGGCC TAAAATACTC TTTAGCTACT GGAAACTGGG GTGATCAAAA GAAAGCTCAT 
CAAGCCAGAG CTGGAGTATC TCAGGTGTTA AACCGCCTGA CTTTTGCGTC TACTCTTTCT 
CACCTGCGTC GTTTAAATTC TCCTATTGGT AGAGACGGCA AGCTAGCAAA ACCAAGACAG 
TTGCATAATA CGTTGTGGGG AATGGTGTGT CCTGCCGAGA CCCCAGAGGG CCATGCTGTA 
GGACTTGTGA AGAATTTAGC CTTGATGGCG TATATTTCAG TTGGATCTCA ACCATCTCCA 
ATTCTGGAAT TTTTAGAAGA ATGGAGTATG GAAAATTTAG AAGAAATTTC TCCTGCAGCT 
ATTGCTGATG CAACCAAGAT TTTTGTTAAT GGCTGCTGGG TTGGAATACA TAAAGATCCC 
GAACAACTTA TGAACACCCT AAGGAAATTG AGACGTCAGA TGGACATCAT TGTGTCTGAA 
GTTTCTATGA TCAGAGATAT TCGAGAGAGG GAGATTCGGA TCTATACGGA TGCAGGCCGT 
ATTTGTAGAC CACTTCTGAT TGTGGAAAAA CAAAAGCTAC TTTTGAAGAA GAGGCATATT 
GACCAATTGA AAGAGAGAGA ATATAACAAC TATAGTTGGC AGGATCTTGT GGCCAGTGGG 
GTAGTGGAGT ATATTGATAC CCTGGAAGAA GAAACAGTGA TGCTTGCAAT GACTCCAGAT 
GATTTACAGG AGAAAGAAGT AGCTTATTGT TCCACATATA CACACTGTGA GATTCATCCC 
TCAATGATCC TTGGTGTCTG TGCATCTATT ATTCCCTTTC CTGATCATAA CCAGTCCCCT 
AGAAACACAT ACCAGTCTGC TATGGGTAAG CAGGCTATGG GAGTTTACAT CACCAACTTC 
CATGTTCGCA TGGACACATT GGCCCATGTT CTCTATTATC CTCAAAAGCC ACTTGTGACT 
ACACGGTCTA TGGAATATCT ACGATTTAGA GAGCTGCCAG CAGGCATCAA CTCAATTGTG 
GCCATTGCAT CATACACTGG ATATAATCAG GAAGACTCTG TTATCATGAA TCGTTCAGCT 
GTAGACCGCG GCTTCTTCAG GTCTGTTTTC TATCGCTCAT ACAAAGAACA GGAGTCTAAA 
AAAGGATTTG ATCAAGAAGA AGTTTTTGAG AAGCCTACAC GTGAAACATG CCAGGGCATG 
AGGCATGCCA TTTACGACAA GCTGGATGAT GATGGTTTGA TAGCTCCAGG GGTTCGTGTA 
TCAGGAGATG ATGTTATTAT AGGCAAAACA GTCACCTTGC CTGAAAATGA AGATGAATTG 
GAGAGCACCA ATAGACGCTA TACCAAGAGA GACTGTAGCA CTTTTCTCAG AACTAGCGAG 
ACGGGCATTG TGGATCAGGT TATGGTAACT CTCAATCAGG AAGGATATAA ATTTTGTAAA 
ATAAGGGTAC GCTCTGTTAG GATTCCACAG ATTGGAGACA AATTTGCTAG TCGACATGGT 
CAAAAGGGTA CTTGTGGTAT TCAGTATAGA CAAGAGGATA TGCCTTTCAC CTGTGAAGGT 
ATCACCCCTG ATATCATCAT CAATCCCCAT GCCATCCCCT CTCGTATGAC TATTGGTCAC 
TTAATTGAAT GCCTTCAAGG GAAGGTATCG GCTAACAAGG GTGAAATTGG TGATGCCACT 
CCATTTAATG ATGCTGTTAA CGTGCAGAAG ATTTCTAATC TTTTATCTGA TTATGGCTAT 
CATCTCAGAG GAAATGAGGT CCTGTACAAT GGGTTCACTG GTCGAAAAAT CACATCACAA 
ATATTTATTG GCCCCACTTA TTACCAGCGT TTGAAGCATA TGGTGGATGA TAAGATTCAC 
TCTCGTGCTA GGGGACCTAT TCAGATCCTC AATAGACAGC CCATGGAGGG TAGATCTCGT 
GATGGTGGCC TGCGTTTTGG AGAAATGGAA CGAGATTGTC AGATTGCCCA TGGAGCAGCC 
CAGTTTTTAA GGGAAAGATT GTTTGAGGCA TCAGATCCAT ATCAGGTTCA TGTTTGCAAT 
CTTTGTGGAA TAATGGCGAT TGCCAACACC AGGACCCATA CATATGAATG CAGGGGCTGC 
CGCAATAAAA CCCAGATTTC TTTGGTGCGA ATGCCTTACG CATGCAAACT ATTGTTTCAG 
GAACTTATGT CTATGAGTAT TGCACCGCGA ATGATGAGTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR2B ( NM_000938.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000929.1 Homo sapiens RNA polymerase II subunit B (POLR2B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu59820
Clone ID Related Accession (Same CDS sequence)	NM_000938.2 , NM_000938.3
Accession Version	NM_000938.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase II subunit RPB2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC023503.2 and AK294901.1. On Nov 23, 2018 this sequence version replaced NM_000938.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.148220.1, BC023503.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000314595.6/ ENSP00000312735.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTACGACG CGGATGAGGA TATGCAATAT GATGAGGATG ATGATGAAAT CACCCCGGAT 
TTGTGGCAAG AAGCATGCTG GATTGTAATC AGTTCCTATT TTGACGAGAA AGGCTTGGTT 
AGACAACAGC TGGATTCTTT TGATGAGTTT ATTCAGATGT CTGTTCAAAG AATTGTGGAA 
GACGCTCCTC CTATAGACCT ACAGGCTGAA GCTCAGCATG CTAGTGGAGA AGTTGAAGAA 
CCGCCACGAT ATTTGCTGAA GTTTGAACAA ATTTATCTTT CCAAGCCTAC CCATTGGGAA 
AGAGATGGTG CTCCTTCACC AATGATGCCC AATGAAGCTA GATTAAGGAA TCTCACGTAT 
TCTGCTCCGC TTTATGTTGA TATAACAAAA ACAGTCATTA AAGAAGGTGA AGAACAACTT 
CAGACTCAGC ATCAGAAAAC TTTTATAGGA AAAATTCCAA TTATGTTGCG GTCAACTTAC 
TGCCTTTTGA ATGGCTTGAC AGATCGTGAT CTTTGTGAGT TAAATGAATG CCCTTTGGAT 
CCTGGTGGCT ATTTCATTAT TAATGGATCA GAAAAGGTTC TGATTGCCCA AGAGAAAATG 
GCAACAAACA CAGTTTATGT GTTTGCCAAA AAGGATTCTA AATATGCCTA CACAGGAGAG 
TGTAGATCAT GTCTTGAGAA TTCTTCCCGA CCCACCAGTA CTATATGGGT TAGCATGCTG 
GCAAGAGGAG GACAGGGTGC CAAGAAGAGT GCTATTGGTC AGCGCATTGT GGCAACTCTA 
CCATATATCA AGCAAGAAGT TCCCATCATT ATTGTGTTCA GAGCATTAGG TTTTGTGTCC 
GACAGAGATA TTTTAGAACA TATTATTTAT GATTTTGAAG ATCCAGAGAT GATGGAAATG 
GTTAAACCTT CTCTCGATGA AGCTTTTGTC ATCCAAGAAC AGAATGTTGC ACTAAATTTC 
ATTGGTTCAA GAGGAGCAAA GCCTGGTGTT ACTAAAGAGA AAAGAATTAA ATATGCAAAG 
GAAGTTTTAC AAAAAGAAAT GCTCCCTCAT GTTGGTGTCA GTGATTTTTG TGAGACCAAA 
AAAGCCTATT TCTTGGGATA CATGGTTCAT AGGTTACTTC TGGCAGCTTT GGGTAGAAGA 
GAACTAGATG ACAGAGATCA CTATGGAAAC AAGAGATTGG ATCTTGCTGG GCCGCTGCTT 
GCATTCTTAT TTAGAGGTAT GTTTAAGAAT TTGCTTAAAG AAGTGCGGAT CTATGCACAG 
AAATTTATTG ATCGAGGAAA GGATTTTAAC TTGGAGTTGG CAATTAAAAC ACGGATCATA 
TCTGATGGCC TAAAATACTC TTTAGCTACT GGAAACTGGG GTGATCAAAA GAAAGCTCAT 
CAAGCCAGAG CTGGAGTATC TCAGGTGTTA AACCGCCTGA CTTTTGCGTC TACTCTTTCT 
CACCTGCGTC GTTTAAATTC TCCTATTGGT AGAGACGGCA AGCTAGCAAA ACCAAGACAG 
TTGCATAATA CGTTGTGGGG AATGGTGTGT CCTGCCGAGA CCCCAGAGGG CCATGCTGTA 
GGACTTGTGA AGAATTTAGC CTTGATGGCG TATATTTCAG TTGGATCTCA ACCATCTCCA 
ATTCTGGAAT TTTTAGAAGA ATGGAGTATG GAAAATTTAG AAGAAATTTC TCCTGCAGCT 
ATTGCTGATG CAACCAAGAT TTTTGTTAAT GGCTGCTGGG TTGGAATACA TAAAGATCCC 
GAACAACTTA TGAACACCCT AAGGAAATTG AGACGTCAGA TGGACATCAT TGTGTCTGAA 
GTTTCTATGA TCAGAGATAT TCGAGAGAGG GAGATTCGGA TCTATACGGA TGCAGGCCGT 
ATTTGTAGAC CACTTCTGAT TGTGGAAAAA CAAAAGCTAC TTTTGAAGAA GAGGCATATT 
GACCAATTGA AAGAGAGAGA ATATAACAAC TATAGTTGGC AGGATCTTGT GGCCAGTGGG 
GTAGTGGAGT ATATTGATAC CCTGGAAGAA GAAACAGTGA TGCTTGCAAT GACTCCAGAT 
GATTTACAGG AGAAAGAAGT AGCTTATTGT TCCACATATA CACACTGTGA GATTCATCCC 
TCAATGATCC TTGGTGTCTG TGCATCTATT ATTCCCTTTC CTGATCATAA CCAGTCCCCT 
AGAAACACAT ACCAGTCTGC TATGGGTAAG CAGGCTATGG GAGTTTACAT CACCAACTTC 
CATGTTCGCA TGGACACATT GGCCCATGTT CTCTATTATC CTCAAAAGCC ACTTGTGACT 
ACACGGTCTA TGGAATATCT ACGATTTAGA GAGCTGCCAG CAGGCATCAA CTCAATTGTG 
GCCATTGCAT CATACACTGG ATATAATCAG GAAGACTCTG TTATCATGAA TCGTTCAGCT 
GTAGACCGCG GCTTCTTCAG GTCTGTTTTC TATCGCTCAT ACAAAGAACA GGAGTCTAAA 
AAAGGATTTG ATCAAGAAGA AGTTTTTGAG AAGCCTACAC GTGAAACATG CCAGGGCATG 
AGGCATGCCA TTTACGACAA GCTGGATGAT GATGGTTTGA TAGCTCCAGG GGTTCGTGTA 
TCAGGAGATG ATGTTATTAT AGGCAAAACA GTCACCTTGC CTGAAAATGA AGATGAATTG 
GAGAGCACCA ATAGACGCTA TACCAAGAGA GACTGTAGCA CTTTTCTCAG AACTAGCGAG 
ACGGGCATTG TGGATCAGGT TATGGTAACT CTCAATCAGG AAGGATATAA ATTTTGTAAA 
ATAAGGGTAC GCTCTGTTAG GATTCCACAG ATTGGAGACA AATTTGCTAG TCGACATGGT 
CAAAAGGGTA CTTGTGGTAT TCAGTATAGA CAAGAGGATA TGCCTTTCAC CTGTGAAGGT 
ATCACCCCTG ATATCATCAT CAATCCCCAT GCCATCCCCT CTCGTATGAC TATTGGTCAC 
TTAATTGAAT GCCTTCAAGG GAAGGTATCG GCTAACAAGG GTGAAATTGG TGATGCCACT 
CCATTTAATG ATGCTGTTAA CGTGCAGAAG ATTTCTAATC TTTTATCTGA TTATGGCTAT 
CATCTCAGAG GAAATGAGGT CCTGTACAAT GGGTTCACTG GTCGAAAAAT CACATCACAA 
ATATTTATTG GCCCCACTTA TTACCAGCGT TTGAAGCATA TGGTGGATGA TAAGATTCAC 
TCTCGTGCTA GGGGACCTAT TCAGATCCTC AATAGACAGC CCATGGAGGG TAGATCTCGT 
GATGGTGGCC TGCGTTTTGG AGAAATGGAA CGAGATTGTC AGATTGCCCA TGGAGCAGCC 
CAGTTTTTAA GGGAAAGATT GTTTGAGGCA TCAGATCCAT ATCAGGTTCA TGTTTGCAAT 
CTTTGTGGAA TAATGGCGAT TGCCAACACC AGGACCCATA CATATGAATG CAGGGGCTGC 
CGCAATAAAA CCCAGATTTC TTTGGTGCGA ATGCCTTACG CATGCAAACT ATTGTTTCAG 
GAACTTATGT CTATGAGTAT TGCACCGCGA ATGATGAGTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000929.1
CDS	91..3615
Translation	MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

Target ORF information:

RefSeq Version	NM_000938.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit B (POLR2B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000938.3

ORF Insert Sequence:

ATGTACGACG CGGATGAGGA TATGCAATAT GATGAGGATG ATGATGAAAT CACCCCGGAT 
TTGTGGCAAG AAGCATGCTG GATTGTAATC AGTTCCTATT TTGACGAGAA AGGCTTGGTT 
AGACAACAGC TGGATTCTTT TGATGAGTTT ATTCAGATGT CTGTTCAAAG AATTGTGGAA 
GACGCTCCTC CTATAGACCT ACAGGCTGAA GCTCAGCATG CTAGTGGAGA AGTTGAAGAA 
CCGCCACGAT ATTTGCTGAA GTTTGAACAA ATTTATCTTT CCAAGCCTAC CCATTGGGAA 
AGAGATGGTG CTCCTTCACC AATGATGCCC AATGAAGCTA GATTAAGGAA TCTCACGTAT 
TCTGCTCCGC TTTATGTTGA TATAACAAAA ACAGTCATTA AAGAAGGTGA AGAACAACTT 
CAGACTCAGC ATCAGAAAAC TTTTATAGGA AAAATTCCAA TTATGTTGCG GTCAACTTAC 
TGCCTTTTGA ATGGCTTGAC AGATCGTGAT CTTTGTGAGT TAAATGAATG CCCTTTGGAT 
CCTGGTGGCT ATTTCATTAT TAATGGATCA GAAAAGGTTC TGATTGCCCA AGAGAAAATG 
GCAACAAACA CAGTTTATGT GTTTGCCAAA AAGGATTCTA AATATGCCTA CACAGGAGAG 
TGTAGATCAT GTCTTGAGAA TTCTTCCCGA CCCACCAGTA CTATATGGGT TAGCATGCTG 
GCAAGAGGAG GACAGGGTGC CAAGAAGAGT GCTATTGGTC AGCGCATTGT GGCAACTCTA 
CCATATATCA AGCAAGAAGT TCCCATCATT ATTGTGTTCA GAGCATTAGG TTTTGTGTCC 
GACAGAGATA TTTTAGAACA TATTATTTAT GATTTTGAAG ATCCAGAGAT GATGGAAATG 
GTTAAACCTT CTCTCGATGA AGCTTTTGTC ATCCAAGAAC AGAATGTTGC ACTAAATTTC 
ATTGGTTCAA GAGGAGCAAA GCCTGGTGTT ACTAAAGAGA AAAGAATTAA ATATGCAAAG 
GAAGTTTTAC AAAAAGAAAT GCTCCCTCAT GTTGGTGTCA GTGATTTTTG TGAGACCAAA 
AAAGCCTATT TCTTGGGATA CATGGTTCAT AGGTTACTTC TGGCAGCTTT GGGTAGAAGA 
GAACTAGATG ACAGAGATCA CTATGGAAAC AAGAGATTGG ATCTTGCTGG GCCGCTGCTT 
GCATTCTTAT TTAGAGGTAT GTTTAAGAAT TTGCTTAAAG AAGTGCGGAT CTATGCACAG 
AAATTTATTG ATCGAGGAAA GGATTTTAAC TTGGAGTTGG CAATTAAAAC ACGGATCATA 
TCTGATGGCC TAAAATACTC TTTAGCTACT GGAAACTGGG GTGATCAAAA GAAAGCTCAT 
CAAGCCAGAG CTGGAGTATC TCAGGTGTTA AACCGCCTGA CTTTTGCGTC TACTCTTTCT 
CACCTGCGTC GTTTAAATTC TCCTATTGGT AGAGACGGCA AGCTAGCAAA ACCAAGACAG 
TTGCATAATA CGTTGTGGGG AATGGTGTGT CCTGCCGAGA CCCCAGAGGG CCATGCTGTA 
GGACTTGTGA AGAATTTAGC CTTGATGGCG TATATTTCAG TTGGATCTCA ACCATCTCCA 
ATTCTGGAAT TTTTAGAAGA ATGGAGTATG GAAAATTTAG AAGAAATTTC TCCTGCAGCT 
ATTGCTGATG CAACCAAGAT TTTTGTTAAT GGCTGCTGGG TTGGAATACA TAAAGATCCC 
GAACAACTTA TGAACACCCT AAGGAAATTG AGACGTCAGA TGGACATCAT TGTGTCTGAA 
GTTTCTATGA TCAGAGATAT TCGAGAGAGG GAGATTCGGA TCTATACGGA TGCAGGCCGT 
ATTTGTAGAC CACTTCTGAT TGTGGAAAAA CAAAAGCTAC TTTTGAAGAA GAGGCATATT 
GACCAATTGA AAGAGAGAGA ATATAACAAC TATAGTTGGC AGGATCTTGT GGCCAGTGGG 
GTAGTGGAGT ATATTGATAC CCTGGAAGAA GAAACAGTGA TGCTTGCAAT GACTCCAGAT 
GATTTACAGG AGAAAGAAGT AGCTTATTGT TCCACATATA CACACTGTGA GATTCATCCC 
TCAATGATCC TTGGTGTCTG TGCATCTATT ATTCCCTTTC CTGATCATAA CCAGTCCCCT 
AGAAACACAT ACCAGTCTGC TATGGGTAAG CAGGCTATGG GAGTTTACAT CACCAACTTC 
CATGTTCGCA TGGACACATT GGCCCATGTT CTCTATTATC CTCAAAAGCC ACTTGTGACT 
ACACGGTCTA TGGAATATCT ACGATTTAGA GAGCTGCCAG CAGGCATCAA CTCAATTGTG 
GCCATTGCAT CATACACTGG ATATAATCAG GAAGACTCTG TTATCATGAA TCGTTCAGCT 
GTAGACCGCG GCTTCTTCAG GTCTGTTTTC TATCGCTCAT ACAAAGAACA GGAGTCTAAA 
AAAGGATTTG ATCAAGAAGA AGTTTTTGAG AAGCCTACAC GTGAAACATG CCAGGGCATG 
AGGCATGCCA TTTACGACAA GCTGGATGAT GATGGTTTGA TAGCTCCAGG GGTTCGTGTA 
TCAGGAGATG ATGTTATTAT AGGCAAAACA GTCACCTTGC CTGAAAATGA AGATGAATTG 
GAGAGCACCA ATAGACGCTA TACCAAGAGA GACTGTAGCA CTTTTCTCAG AACTAGCGAG 
ACGGGCATTG TGGATCAGGT TATGGTAACT CTCAATCAGG AAGGATATAA ATTTTGTAAA 
ATAAGGGTAC GCTCTGTTAG GATTCCACAG ATTGGAGACA AATTTGCTAG TCGACATGGT 
CAAAAGGGTA CTTGTGGTAT TCAGTATAGA CAAGAGGATA TGCCTTTCAC CTGTGAAGGT 
ATCACCCCTG ATATCATCAT CAATCCCCAT GCCATCCCCT CTCGTATGAC TATTGGTCAC 
TTAATTGAAT GCCTTCAAGG GAAGGTATCG GCTAACAAGG GTGAAATTGG TGATGCCACT 
CCATTTAATG ATGCTGTTAA CGTGCAGAAG ATTTCTAATC TTTTATCTGA TTATGGCTAT 
CATCTCAGAG GAAATGAGGT CCTGTACAAT GGGTTCACTG GTCGAAAAAT CACATCACAA 
ATATTTATTG GCCCCACTTA TTACCAGCGT TTGAAGCATA TGGTGGATGA TAAGATTCAC 
TCTCGTGCTA GGGGACCTAT TCAGATCCTC AATAGACAGC CCATGGAGGG TAGATCTCGT 
GATGGTGGCC TGCGTTTTGG AGAAATGGAA CGAGATTGTC AGATTGCCCA TGGAGCAGCC 
CAGTTTTTAA GGGAAAGATT GTTTGAGGCA TCAGATCCAT ATCAGGTTCA TGTTTGCAAT 
CTTTGTGGAA TAATGGCGAT TGCCAACACC AGGACCCATA CATATGAATG CAGGGGCTGC 
CGCAATAAAA CCCAGATTTC TTTGGTGCGA ATGCCTTACG CATGCAAACT ATTGTTTCAG 
GAACTTATGT CTATGAGTAT TGCACCGCGA ATGATGAGTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR2B ( NM_001303268.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001290197.1 Homo sapiens RNA polymerase II subunit B (POLR2B), transcript variant 2, mRNA.

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CloneID	OHu59821
Clone ID Related Accession (Same CDS sequence)	NM_001303268.1
Accession Version	NM_001303268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase II subunit RPB2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC323029.1, AK294901.1, BC023503.2 and BU619780.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks an alternate exon in the 5' coding region, and initiates translation at an alternate downstream start codon, compared to variant 1. This results in isoform 2, which is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294901.1, SRR1803612.94263.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGGATT GTAATCAGCC ACGATATTTG CTGAAGTTTG AACAAATTTA TCTTTCCAAG 
CCTACCCATT GGGAAAGAGA TGGTGCTCCT TCACCAATGA TGCCCAATGA AGCTAGATTA 
AGGAATCTCA CGTATTCTGC TCCGCTTTAT GTTGATATAA CAAAAACAGT CATTAAAGAA 
GGTGAAGAAC AACTTCAGAC TCAGCATCAG AAAACTTTTA TAGGAAAAAT TCCAATTATG 
TTGCGGTCAA CTTACTGCCT TTTGAATGGC TTGACAGATC GTGATCTTTG TGAGTTAAAT 
GAATGCCCTT TGGATCCTGG TGGCTATTTC ATTATTAATG GATCAGAAAA GGTTCTGATT 
GCCCAAGAGA AAATGGCAAC AAACACAGTT TATGTGTTTG CCAAAAAGGA TTCTAAATAT 
GCCTACACAG GAGAGTGTAG ATCATGTCTT GAGAATTCTT CCCGACCCAC CAGTACTATA 
TGGGTTAGCA TGCTGGCAAG AGGAGGACAG GGTGCCAAGA AGAGTGCTAT TGGTCAGCGC 
ATTGTGGCAA CTCTACCATA TATCAAGCAA GAAGTTCCCA TCATTATTGT GTTCAGAGCA 
TTAGGTTTTG TGTCCGACAG AGATATTTTA GAACATATTA TTTATGATTT TGAAGATCCA 
GAGATGATGG AAATGGTTAA ACCTTCTCTC GATGAAGCTT TTGTCATCCA AGAACAGAAT 
GTTGCACTAA ATTTCATTGG TTCAAGAGGA GCAAAGCCTG GTGTTACTAA AGAGAAAAGA 
ATTAAATATG CAAAGGAAGT TTTACAAAAA GAAATGCTCC CTCATGTTGG TGTCAGTGAT 
TTTTGTGAGA CCAAAAAAGC CTATTTCTTG GGATACATGG TTCATAGGTT ACTTCTGGCA 
GCTTTGGGTA GAAGAGAACT AGATGACAGA GATCACTATG GAAACAAGAG ATTGGATCTT 
GCTGGGCCGC TGCTTGCATT CTTATTTAGA GGTATGTTTA AGAATTTGCT TAAAGAAGTG 
CGGATCTATG CACAGAAATT TATTGATCGA GGAAAGGATT TTAACTTGGA GTTGGCAATT 
AAAACACGGA TCATATCTGA TGGCCTAAAA TACTCTTTAG CTACTGGAAA CTGGGGTGAT 
CAAAAGAAAG CTCATCAAGC CAGAGCTGGA GTATCTCAGG TGTTAAACCG CCTGACTTTT 
GCGTCTACTC TTTCTCACCT GCGTCGTTTA AATTCTCCTA TTGGTAGAGA CGGCAAGCTA 
GCAAAACCAA GACAGTTGCA TAATACGTTG TGGGGAATGG TGTGTCCTGC CGAGACCCCA 
GAGGGCCATG CTGTAGGACT TGTGAAGAAT TTAGCCTTGA TGGCGTATAT TTCAGTTGGA 
TCTCAACCAT CTCCAATTCT GGAATTTTTA GAAGAATGGA GTATGGAAAA TTTAGAAGAA 
ATTTCTCCTG CAGCTATTGC TGATGCAACC AAGATTTTTG TTAATGGCTG CTGGGTTGGA 
ATACATAAAG ATCCCGAACA ACTTATGAAC ACCCTAAGGA AATTGAGACG TCAGATGGAC 
ATCATTGTGT CTGAAGTTTC TATGATCAGA GATATTCGAG AGAGGGAGAT TCGGATCTAT 
ACGGATGCAG GCCGTATTTG TAGACCACTT CTGATTGTGG AAAAACAAAA GCTACTTTTG 
AAGAAGAGGC ATATTGACCA ATTGAAAGAG AGAGAATATA ACAACTATAG TTGGCAGGAT 
CTTGTGGCCA GTGGGGTAGT GGAGTATATT GATACCCTGG AAGAAGAAAC AGTGATGCTT 
GCAATGACTC CAGATGATTT ACAGGAGAAA GAAGTAGCTT ATTGTTCCAC ATATACACAC 
TGTGAGATTC ATCCCTCAAT GATCCTTGGT GTCTGTGCAT CTATTATTCC CTTTCCTGAT 
CATAACCAGT CCCCTAGAAA CACATACCAG TCTGCTATGG GTAAGCAGGC TATGGGAGTT 
TACATCACCA ACTTCCATGT TCGCATGGAC ACATTGGCCC ATGTTCTCTA TTATCCTCAA 
AAGCCACTTG TGACTACACG GTCTATGGAA TATCTACGAT TTAGAGAGCT GCCAGCAGGC 
ATCAACTCAA TTGTGGCCAT TGCATCATAC ACTGGATATA ATCAGGAAGA CTCTGTTATC 
ATGAATCGTT CAGCTGTAGA CCGCGGCTTC TTCAGGTCTG TTTTCTATCG CTCATACAAA 
GAACAGGAGT CTAAAAAAGG ATTTGATCAA GAAGAAGTTT TTGAGAAGCC TACACGTGAA 
ACATGCCAGG GCATGAGGCA TGCCATTTAC GACAAGCTGG ATGATGATGG TTTGATAGCT 
CCAGGGGTTC GTGTATCAGG AGATGATGTT ATTATAGGCA AAACAGTCAC CTTGCCTGAA 
AATGAAGATG AATTGGAGAG CACCAATAGA CGCTATACCA AGAGAGACTG TAGCACTTTT 
CTCAGAACTA GCGAGACGGG CATTGTGGAT CAGGTTATGG TAACTCTCAA TCAGGAAGGA 
TATAAATTTT GTAAAATAAG GGTACGCTCT GTTAGGATTC CACAGATTGG AGACAAATTT 
GCTAGTCGAC ATGGTCAAAA GGGTACTTGT GGTATTCAGT ATAGACAAGA GGATATGCCT 
TTCACCTGTG AAGGTATCAC CCCTGATATC ATCATCAATC CCCATGCCAT CCCCTCTCGT 
ATGACTATTG GTCACTTAAT TGAATGCCTT CAAGGGAAGG TATCGGCTAA CAAGGGTGAA 
ATTGGTGATG CCACTCCATT TAATGATGCT GTTAACGTGC AGAAGATTTC TAATCTTTTA 
TCTGATTATG GCTATCATCT CAGAGGAAAT GAGGTCCTGT ACAATGGGTT CACTGGTCGA 
AAAATCACAT CACAAATATT TATTGGCCCC ACTTATTACC AGCGTTTGAA GCATATGGTG 
GATGATAAGA TTCACTCTCG TGCTAGGGGA CCTATTCAGA TCCTCAATAG ACAGCCCATG 
GAGGGTAGAT CTCGTGATGG TGGCCTGCGT TTTGGAGAAA TGGAACGAGA TTGTCAGATT 
GCCCATGGAG CAGCCCAGTT TTTAAGGGAA AGATTGTTTG AGGCATCAGA TCCATATCAG 
GTTCATGTTT GCAATCTTTG TGGAATAATG GCGATTGCCA ACACCAGGAC CCATACATAT 
GAATGCAGGG GCTGCCGCAA TAAAACCCAG ATTTCTTTGG TGCGAATGCC TTACGCATGC 
AAACTATTGT TTCAGGAACT TATGTCTATG AGTATTGCAC CGCGAATGAT GAGTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290197.1
CDS	421..3720
Translation	MLDCNQPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

Target ORF information:

RefSeq Version	NM_001303268.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit B (POLR2B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303268.1

ORF Insert Sequence:

ATGCTGGATT GTAATCAGCC ACGATATTTG CTGAAGTTTG AACAAATTTA TCTTTCCAAG 
CCTACCCATT GGGAAAGAGA TGGTGCTCCT TCACCAATGA TGCCCAATGA AGCTAGATTA 
AGGAATCTCA CGTATTCTGC TCCGCTTTAT GTTGATATAA CAAAAACAGT CATTAAAGAA 
GGTGAAGAAC AACTTCAGAC TCAGCATCAG AAAACTTTTA TAGGAAAAAT TCCAATTATG 
TTGCGGTCAA CTTACTGCCT TTTGAATGGC TTGACAGATC GTGATCTTTG TGAGTTAAAT 
GAATGCCCTT TGGATCCTGG TGGCTATTTC ATTATTAATG GATCAGAAAA GGTTCTGATT 
GCCCAAGAGA AAATGGCAAC AAACACAGTT TATGTGTTTG CCAAAAAGGA TTCTAAATAT 
GCCTACACAG GAGAGTGTAG ATCATGTCTT GAGAATTCTT CCCGACCCAC CAGTACTATA 
TGGGTTAGCA TGCTGGCAAG AGGAGGACAG GGTGCCAAGA AGAGTGCTAT TGGTCAGCGC 
ATTGTGGCAA CTCTACCATA TATCAAGCAA GAAGTTCCCA TCATTATTGT GTTCAGAGCA 
TTAGGTTTTG TGTCCGACAG AGATATTTTA GAACATATTA TTTATGATTT TGAAGATCCA 
GAGATGATGG AAATGGTTAA ACCTTCTCTC GATGAAGCTT TTGTCATCCA AGAACAGAAT 
GTTGCACTAA ATTTCATTGG TTCAAGAGGA GCAAAGCCTG GTGTTACTAA AGAGAAAAGA 
ATTAAATATG CAAAGGAAGT TTTACAAAAA GAAATGCTCC CTCATGTTGG TGTCAGTGAT 
TTTTGTGAGA CCAAAAAAGC CTATTTCTTG GGATACATGG TTCATAGGTT ACTTCTGGCA 
GCTTTGGGTA GAAGAGAACT AGATGACAGA GATCACTATG GAAACAAGAG ATTGGATCTT 
GCTGGGCCGC TGCTTGCATT CTTATTTAGA GGTATGTTTA AGAATTTGCT TAAAGAAGTG 
CGGATCTATG CACAGAAATT TATTGATCGA GGAAAGGATT TTAACTTGGA GTTGGCAATT 
AAAACACGGA TCATATCTGA TGGCCTAAAA TACTCTTTAG CTACTGGAAA CTGGGGTGAT 
CAAAAGAAAG CTCATCAAGC CAGAGCTGGA GTATCTCAGG TGTTAAACCG CCTGACTTTT 
GCGTCTACTC TTTCTCACCT GCGTCGTTTA AATTCTCCTA TTGGTAGAGA CGGCAAGCTA 
GCAAAACCAA GACAGTTGCA TAATACGTTG TGGGGAATGG TGTGTCCTGC CGAGACCCCA 
GAGGGCCATG CTGTAGGACT TGTGAAGAAT TTAGCCTTGA TGGCGTATAT TTCAGTTGGA 
TCTCAACCAT CTCCAATTCT GGAATTTTTA GAAGAATGGA GTATGGAAAA TTTAGAAGAA 
ATTTCTCCTG CAGCTATTGC TGATGCAACC AAGATTTTTG TTAATGGCTG CTGGGTTGGA 
ATACATAAAG ATCCCGAACA ACTTATGAAC ACCCTAAGGA AATTGAGACG TCAGATGGAC 
ATCATTGTGT CTGAAGTTTC TATGATCAGA GATATTCGAG AGAGGGAGAT TCGGATCTAT 
ACGGATGCAG GCCGTATTTG TAGACCACTT CTGATTGTGG AAAAACAAAA GCTACTTTTG 
AAGAAGAGGC ATATTGACCA ATTGAAAGAG AGAGAATATA ACAACTATAG TTGGCAGGAT 
CTTGTGGCCA GTGGGGTAGT GGAGTATATT GATACCCTGG AAGAAGAAAC AGTGATGCTT 
GCAATGACTC CAGATGATTT ACAGGAGAAA GAAGTAGCTT ATTGTTCCAC ATATACACAC 
TGTGAGATTC ATCCCTCAAT GATCCTTGGT GTCTGTGCAT CTATTATTCC CTTTCCTGAT 
CATAACCAGT CCCCTAGAAA CACATACCAG TCTGCTATGG GTAAGCAGGC TATGGGAGTT 
TACATCACCA ACTTCCATGT TCGCATGGAC ACATTGGCCC ATGTTCTCTA TTATCCTCAA 
AAGCCACTTG TGACTACACG GTCTATGGAA TATCTACGAT TTAGAGAGCT GCCAGCAGGC 
ATCAACTCAA TTGTGGCCAT TGCATCATAC ACTGGATATA ATCAGGAAGA CTCTGTTATC 
ATGAATCGTT CAGCTGTAGA CCGCGGCTTC TTCAGGTCTG TTTTCTATCG CTCATACAAA 
GAACAGGAGT CTAAAAAAGG ATTTGATCAA GAAGAAGTTT TTGAGAAGCC TACACGTGAA 
ACATGCCAGG GCATGAGGCA TGCCATTTAC GACAAGCTGG ATGATGATGG TTTGATAGCT 
CCAGGGGTTC GTGTATCAGG AGATGATGTT ATTATAGGCA AAACAGTCAC CTTGCCTGAA 
AATGAAGATG AATTGGAGAG CACCAATAGA CGCTATACCA AGAGAGACTG TAGCACTTTT 
CTCAGAACTA GCGAGACGGG CATTGTGGAT CAGGTTATGG TAACTCTCAA TCAGGAAGGA 
TATAAATTTT GTAAAATAAG GGTACGCTCT GTTAGGATTC CACAGATTGG AGACAAATTT 
GCTAGTCGAC ATGGTCAAAA GGGTACTTGT GGTATTCAGT ATAGACAAGA GGATATGCCT 
TTCACCTGTG AAGGTATCAC CCCTGATATC ATCATCAATC CCCATGCCAT CCCCTCTCGT 
ATGACTATTG GTCACTTAAT TGAATGCCTT CAAGGGAAGG TATCGGCTAA CAAGGGTGAA 
ATTGGTGATG CCACTCCATT TAATGATGCT GTTAACGTGC AGAAGATTTC TAATCTTTTA 
TCTGATTATG GCTATCATCT CAGAGGAAAT GAGGTCCTGT ACAATGGGTT CACTGGTCGA 
AAAATCACAT CACAAATATT TATTGGCCCC ACTTATTACC AGCGTTTGAA GCATATGGTG 
GATGATAAGA TTCACTCTCG TGCTAGGGGA CCTATTCAGA TCCTCAATAG ACAGCCCATG 
GAGGGTAGAT CTCGTGATGG TGGCCTGCGT TTTGGAGAAA TGGAACGAGA TTGTCAGATT 
GCCCATGGAG CAGCCCAGTT TTTAAGGGAA AGATTGTTTG AGGCATCAGA TCCATATCAG 
GTTCATGTTT GCAATCTTTG TGGAATAATG GCGATTGCCA ACACCAGGAC CCATACATAT 
GAATGCAGGG GCTGCCGCAA TAAAACCCAG ATTTCTTTGG TGCGAATGCC TTACGCATGC 
AAACTATTGT TTCAGGAACT TATGTCTATG AGTATTGCAC CGCGAATGAT GAGTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR2B ( NM_001303269.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001290198.1 Homo sapiens RNA polymerase II subunit B (POLR2B), transcript variant 3, mRNA.

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CloneID	OHu59822
Clone ID Related Accession (Same CDS sequence)	NM_001303269.1
Accession Version	NM_001303269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase II subunit RPB2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC323029.1, AK295132.1, BC023503.2, AK294901.1 and BU619780.1. Transcript Variant: This variant (3) contains an alternate exon in its 5' UTR and initiates translation at an in-frame downstream start codon, compared to variant 1. This results in isoform 3, which has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.61967.1, SRR1803612.147724.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAATATG ATGAGGATGA TGATGAAATC ACCCCGGATT TGTGGCAAGA AGCATGCTGG 
ATTGTAATCA GTTCCTATTT TGACGAGAAA GGCTTGGTTA GACAACAGCT GGATTCTTTT 
GATGAGTTTA TTCAGATGTC TGTTCAAAGA ATTGTGGAAG ACGCTCCTCC TATAGACCTA 
CAGGCTGAAG CTCAGCATGC TAGTGGAGAA GTTGAAGAAC CGCCACGATA TTTGCTGAAG 
TTTGAACAAA TTTATCTTTC CAAGCCTACC CATTGGGAAA GAGATGGTGC TCCTTCACCA 
ATGATGCCCA ATGAAGCTAG ATTAAGGAAT CTCACGTATT CTGCTCCGCT TTATGTTGAT 
ATAACAAAAA CAGTCATTAA AGAAGGTGAA GAACAACTTC AGACTCAGCA TCAGAAAACT 
TTTATAGGAA AAATTCCAAT TATGTTGCGG TCAACTTACT GCCTTTTGAA TGGCTTGACA 
GATCGTGATC TTTGTGAGTT AAATGAATGC CCTTTGGATC CTGGTGGCTA TTTCATTATT 
AATGGATCAG AAAAGGTTCT GATTGCCCAA GAGAAAATGG CAACAAACAC AGTTTATGTG 
TTTGCCAAAA AGGATTCTAA ATATGCCTAC ACAGGAGAGT GTAGATCATG TCTTGAGAAT 
TCTTCCCGAC CCACCAGTAC TATATGGGTT AGCATGCTGG CAAGAGGAGG ACAGGGTGCC 
AAGAAGAGTG CTATTGGTCA GCGCATTGTG GCAACTCTAC CATATATCAA GCAAGAAGTT 
CCCATCATTA TTGTGTTCAG AGCATTAGGT TTTGTGTCCG ACAGAGATAT TTTAGAACAT 
ATTATTTATG ATTTTGAAGA TCCAGAGATG ATGGAAATGG TTAAACCTTC TCTCGATGAA 
GCTTTTGTCA TCCAAGAACA GAATGTTGCA CTAAATTTCA TTGGTTCAAG AGGAGCAAAG 
CCTGGTGTTA CTAAAGAGAA AAGAATTAAA TATGCAAAGG AAGTTTTACA AAAAGAAATG 
CTCCCTCATG TTGGTGTCAG TGATTTTTGT GAGACCAAAA AAGCCTATTT CTTGGGATAC 
ATGGTTCATA GGTTACTTCT GGCAGCTTTG GGTAGAAGAG AACTAGATGA CAGAGATCAC 
TATGGAAACA AGAGATTGGA TCTTGCTGGG CCGCTGCTTG CATTCTTATT TAGAGGTATG 
TTTAAGAATT TGCTTAAAGA AGTGCGGATC TATGCACAGA AATTTATTGA TCGAGGAAAG 
GATTTTAACT TGGAGTTGGC AATTAAAACA CGGATCATAT CTGATGGCCT AAAATACTCT 
TTAGCTACTG GAAACTGGGG TGATCAAAAG AAAGCTCATC AAGCCAGAGC TGGAGTATCT 
CAGGTGTTAA ACCGCCTGAC TTTTGCGTCT ACTCTTTCTC ACCTGCGTCG TTTAAATTCT 
CCTATTGGTA GAGACGGCAA GCTAGCAAAA CCAAGACAGT TGCATAATAC GTTGTGGGGA 
ATGGTGTGTC CTGCCGAGAC CCCAGAGGGC CATGCTGTAG GACTTGTGAA GAATTTAGCC 
TTGATGGCGT ATATTTCAGT TGGATCTCAA CCATCTCCAA TTCTGGAATT TTTAGAAGAA 
TGGAGTATGG AAAATTTAGA AGAAATTTCT CCTGCAGCTA TTGCTGATGC AACCAAGATT 
TTTGTTAATG GCTGCTGGGT TGGAATACAT AAAGATCCCG AACAACTTAT GAACACCCTA 
AGGAAATTGA GACGTCAGAT GGACATCATT GTGTCTGAAG TTTCTATGAT CAGAGATATT 
CGAGAGAGGG AGATTCGGAT CTATACGGAT GCAGGCCGTA TTTGTAGACC ACTTCTGATT 
GTGGAAAAAC AAAAGCTACT TTTGAAGAAG AGGCATATTG ACCAATTGAA AGAGAGAGAA 
TATAACAACT ATAGTTGGCA GGATCTTGTG GCCAGTGGGG TAGTGGAGTA TATTGATACC 
CTGGAAGAAG AAACAGTGAT GCTTGCAATG ACTCCAGATG ATTTACAGGA GAAAGAAGTA 
GCTTATTGTT CCACATATAC ACACTGTGAG ATTCATCCCT CAATGATCCT TGGTGTCTGT 
GCATCTATTA TTCCCTTTCC TGATCATAAC CAGTCCCCTA GAAACACATA CCAGTCTGCT 
ATGGGTAAGC AGGCTATGGG AGTTTACATC ACCAACTTCC ATGTTCGCAT GGACACATTG 
GCCCATGTTC TCTATTATCC TCAAAAGCCA CTTGTGACTA CACGGTCTAT GGAATATCTA 
CGATTTAGAG AGCTGCCAGC AGGCATCAAC TCAATTGTGG CCATTGCATC ATACACTGGA 
TATAATCAGG AAGACTCTGT TATCATGAAT CGTTCAGCTG TAGACCGCGG CTTCTTCAGG 
TCTGTTTTCT ATCGCTCATA CAAAGAACAG GAGTCTAAAA AAGGATTTGA TCAAGAAGAA 
GTTTTTGAGA AGCCTACACG TGAAACATGC CAGGGCATGA GGCATGCCAT TTACGACAAG 
CTGGATGATG ATGGTTTGAT AGCTCCAGGG GTTCGTGTAT CAGGAGATGA TGTTATTATA 
GGCAAAACAG TCACCTTGCC TGAAAATGAA GATGAATTGG AGAGCACCAA TAGACGCTAT 
ACCAAGAGAG ACTGTAGCAC TTTTCTCAGA ACTAGCGAGA CGGGCATTGT GGATCAGGTT 
ATGGTAACTC TCAATCAGGA AGGATATAAA TTTTGTAAAA TAAGGGTACG CTCTGTTAGG 
ATTCCACAGA TTGGAGACAA ATTTGCTAGT CGACATGGTC AAAAGGGTAC TTGTGGTATT 
CAGTATAGAC AAGAGGATAT GCCTTTCACC TGTGAAGGTA TCACCCCTGA TATCATCATC 
AATCCCCATG CCATCCCCTC TCGTATGACT ATTGGTCACT TAATTGAATG CCTTCAAGGG 
AAGGTATCGG CTAACAAGGG TGAAATTGGT GATGCCACTC CATTTAATGA TGCTGTTAAC 
GTGCAGAAGA TTTCTAATCT TTTATCTGAT TATGGCTATC ATCTCAGAGG AAATGAGGTC 
CTGTACAATG GGTTCACTGG TCGAAAAATC ACATCACAAA TATTTATTGG CCCCACTTAT 
TACCAGCGTT TGAAGCATAT GGTGGATGAT AAGATTCACT CTCGTGCTAG GGGACCTATT 
CAGATCCTCA ATAGACAGCC CATGGAGGGT AGATCTCGTG ATGGTGGCCT GCGTTTTGGA 
GAAATGGAAC GAGATTGTCA GATTGCCCAT GGAGCAGCCC AGTTTTTAAG GGAAAGATTG 
TTTGAGGCAT CAGATCCATA TCAGGTTCAT GTTTGCAATC TTTGTGGAAT AATGGCGATT 
GCCAACACCA GGACCCATAC ATATGAATGC AGGGGCTGCC GCAATAAAAC CCAGATTTCT 
TTGGTGCGAA TGCCTTACGC ATGCAAACTA TTGTTTCAGG AACTTATGTC TATGAGTATT 
GCACCGCGAA TGATGAGTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290198.1
CDS	546..4049
Translation	MQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

Target ORF information:

RefSeq Version	NM_001303269.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit B (POLR2B), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303269.1

ORF Insert Sequence:

ATGCAATATG ATGAGGATGA TGATGAAATC ACCCCGGATT TGTGGCAAGA AGCATGCTGG 
ATTGTAATCA GTTCCTATTT TGACGAGAAA GGCTTGGTTA GACAACAGCT GGATTCTTTT 
GATGAGTTTA TTCAGATGTC TGTTCAAAGA ATTGTGGAAG ACGCTCCTCC TATAGACCTA 
CAGGCTGAAG CTCAGCATGC TAGTGGAGAA GTTGAAGAAC CGCCACGATA TTTGCTGAAG 
TTTGAACAAA TTTATCTTTC CAAGCCTACC CATTGGGAAA GAGATGGTGC TCCTTCACCA 
ATGATGCCCA ATGAAGCTAG ATTAAGGAAT CTCACGTATT CTGCTCCGCT TTATGTTGAT 
ATAACAAAAA CAGTCATTAA AGAAGGTGAA GAACAACTTC AGACTCAGCA TCAGAAAACT 
TTTATAGGAA AAATTCCAAT TATGTTGCGG TCAACTTACT GCCTTTTGAA TGGCTTGACA 
GATCGTGATC TTTGTGAGTT AAATGAATGC CCTTTGGATC CTGGTGGCTA TTTCATTATT 
AATGGATCAG AAAAGGTTCT GATTGCCCAA GAGAAAATGG CAACAAACAC AGTTTATGTG 
TTTGCCAAAA AGGATTCTAA ATATGCCTAC ACAGGAGAGT GTAGATCATG TCTTGAGAAT 
TCTTCCCGAC CCACCAGTAC TATATGGGTT AGCATGCTGG CAAGAGGAGG ACAGGGTGCC 
AAGAAGAGTG CTATTGGTCA GCGCATTGTG GCAACTCTAC CATATATCAA GCAAGAAGTT 
CCCATCATTA TTGTGTTCAG AGCATTAGGT TTTGTGTCCG ACAGAGATAT TTTAGAACAT 
ATTATTTATG ATTTTGAAGA TCCAGAGATG ATGGAAATGG TTAAACCTTC TCTCGATGAA 
GCTTTTGTCA TCCAAGAACA GAATGTTGCA CTAAATTTCA TTGGTTCAAG AGGAGCAAAG 
CCTGGTGTTA CTAAAGAGAA AAGAATTAAA TATGCAAAGG AAGTTTTACA AAAAGAAATG 
CTCCCTCATG TTGGTGTCAG TGATTTTTGT GAGACCAAAA AAGCCTATTT CTTGGGATAC 
ATGGTTCATA GGTTACTTCT GGCAGCTTTG GGTAGAAGAG AACTAGATGA CAGAGATCAC 
TATGGAAACA AGAGATTGGA TCTTGCTGGG CCGCTGCTTG CATTCTTATT TAGAGGTATG 
TTTAAGAATT TGCTTAAAGA AGTGCGGATC TATGCACAGA AATTTATTGA TCGAGGAAAG 
GATTTTAACT TGGAGTTGGC AATTAAAACA CGGATCATAT CTGATGGCCT AAAATACTCT 
TTAGCTACTG GAAACTGGGG TGATCAAAAG AAAGCTCATC AAGCCAGAGC TGGAGTATCT 
CAGGTGTTAA ACCGCCTGAC TTTTGCGTCT ACTCTTTCTC ACCTGCGTCG TTTAAATTCT 
CCTATTGGTA GAGACGGCAA GCTAGCAAAA CCAAGACAGT TGCATAATAC GTTGTGGGGA 
ATGGTGTGTC CTGCCGAGAC CCCAGAGGGC CATGCTGTAG GACTTGTGAA GAATTTAGCC 
TTGATGGCGT ATATTTCAGT TGGATCTCAA CCATCTCCAA TTCTGGAATT TTTAGAAGAA 
TGGAGTATGG AAAATTTAGA AGAAATTTCT CCTGCAGCTA TTGCTGATGC AACCAAGATT 
TTTGTTAATG GCTGCTGGGT TGGAATACAT AAAGATCCCG AACAACTTAT GAACACCCTA 
AGGAAATTGA GACGTCAGAT GGACATCATT GTGTCTGAAG TTTCTATGAT CAGAGATATT 
CGAGAGAGGG AGATTCGGAT CTATACGGAT GCAGGCCGTA TTTGTAGACC ACTTCTGATT 
GTGGAAAAAC AAAAGCTACT TTTGAAGAAG AGGCATATTG ACCAATTGAA AGAGAGAGAA 
TATAACAACT ATAGTTGGCA GGATCTTGTG GCCAGTGGGG TAGTGGAGTA TATTGATACC 
CTGGAAGAAG AAACAGTGAT GCTTGCAATG ACTCCAGATG ATTTACAGGA GAAAGAAGTA 
GCTTATTGTT CCACATATAC ACACTGTGAG ATTCATCCCT CAATGATCCT TGGTGTCTGT 
GCATCTATTA TTCCCTTTCC TGATCATAAC CAGTCCCCTA GAAACACATA CCAGTCTGCT 
ATGGGTAAGC AGGCTATGGG AGTTTACATC ACCAACTTCC ATGTTCGCAT GGACACATTG 
GCCCATGTTC TCTATTATCC TCAAAAGCCA CTTGTGACTA CACGGTCTAT GGAATATCTA 
CGATTTAGAG AGCTGCCAGC AGGCATCAAC TCAATTGTGG CCATTGCATC ATACACTGGA 
TATAATCAGG AAGACTCTGT TATCATGAAT CGTTCAGCTG TAGACCGCGG CTTCTTCAGG 
TCTGTTTTCT ATCGCTCATA CAAAGAACAG GAGTCTAAAA AAGGATTTGA TCAAGAAGAA 
GTTTTTGAGA AGCCTACACG TGAAACATGC CAGGGCATGA GGCATGCCAT TTACGACAAG 
CTGGATGATG ATGGTTTGAT AGCTCCAGGG GTTCGTGTAT CAGGAGATGA TGTTATTATA 
GGCAAAACAG TCACCTTGCC TGAAAATGAA GATGAATTGG AGAGCACCAA TAGACGCTAT 
ACCAAGAGAG ACTGTAGCAC TTTTCTCAGA ACTAGCGAGA CGGGCATTGT GGATCAGGTT 
ATGGTAACTC TCAATCAGGA AGGATATAAA TTTTGTAAAA TAAGGGTACG CTCTGTTAGG 
ATTCCACAGA TTGGAGACAA ATTTGCTAGT CGACATGGTC AAAAGGGTAC TTGTGGTATT 
CAGTATAGAC AAGAGGATAT GCCTTTCACC TGTGAAGGTA TCACCCCTGA TATCATCATC 
AATCCCCATG CCATCCCCTC TCGTATGACT ATTGGTCACT TAATTGAATG CCTTCAAGGG 
AAGGTATCGG CTAACAAGGG TGAAATTGGT GATGCCACTC CATTTAATGA TGCTGTTAAC 
GTGCAGAAGA TTTCTAATCT TTTATCTGAT TATGGCTATC ATCTCAGAGG AAATGAGGTC 
CTGTACAATG GGTTCACTGG TCGAAAAATC ACATCACAAA TATTTATTGG CCCCACTTAT 
TACCAGCGTT TGAAGCATAT GGTGGATGAT AAGATTCACT CTCGTGCTAG GGGACCTATT 
CAGATCCTCA ATAGACAGCC CATGGAGGGT AGATCTCGTG ATGGTGGCCT GCGTTTTGGA 
GAAATGGAAC GAGATTGTCA GATTGCCCAT GGAGCAGCCC AGTTTTTAAG GGAAAGATTG 
TTTGAGGCAT CAGATCCATA TCAGGTTCAT GTTTGCAATC TTTGTGGAAT AATGGCGATT 
GCCAACACCA GGACCCATAC ATATGAATGC AGGGGCTGCC GCAATAAAAC CCAGATTTCT 
TTGGTGCGAA TGCCTTACGC ATGCAAACTA TTGTTTCAGG AACTTATGTC TATGAGTATT 
GCACCGCGAA TGATGAGTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Meta-analysis of genome-wide association studies of adult height in East Asians identifies 17 novel loci.
Human molecular genetics24(6)1791-800(2015 Mar)
He M,Xu M,Zhang B,Liang J,Chen P,Lee JY,Johnson TA,Li H,Yang X,Dai J,Liang L,Gui L,Qi Q,Huang J,Li Y,Adair LS,Aung T,Cai Q,Cheng CY,Cho MC,Cho YS,Chu M,Cui B,Gao YT,Go MJ,Gu D,Gu W,Guo H,Hao Y,Hong J,Hu Z,Hu Y,Huang J,Hwang JY,Ikram MK,Jin G,Kang DH,Khor CC,Kim BJ,Kim HT,Kubo M,Lee J,Lee J,Lee NR,Li R,Li J,Liu J,Longe J,Lu W,Lu X,Miao X,Okada Y,Ong RT,Qiu G,Seielstad M,Sim X,Song H,Takeuchi F,Tanaka T,Taylor PR,Wang L,Wang W,Wang Y,Wu C,Wu Y,Xiang YB,Yamamoto K,Yang H,Liao M,Yokota M,Young T,Zhang X,Kato N,Wang QK,Zheng W,Hu FB,Lin D,Shen H,Teo YY,Mo Z,Wong TY,Lin X,Mohlke KL,Ning G,Tsunoda T,Han BG,Shu XO,Tai ES,Wu T,Qi L

A quantitative telomeric chromatin isolation protocol identifies different telomeric states.
Nature communications42848(2013)
Grolimund L,Aeby E,Hamelin R,Armand F,Chiappe D,Moniatte M,Lingner J

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Genome-wide association study identifies two susceptibility loci for exudative age-related macular degeneration in the Japanese population.
Nature genetics43(10)1001-4(2011 Sep)
Arakawa S,Takahashi A,Ashikawa K,Hosono N,Aoi T,Yasuda M,Oshima Y,Yoshida S,Enaida H,Tsuchihashi T,Mori K,Honda S,Negi A,Arakawa A,Kadonosono K,Kiyohara Y,Kamatani N,Nakamura Y,Ishibashi T,Kubo M

The RPB2 flap loop of human RNA polymerase II is dispensable for transcription initiation and elongation.
Molecular and cellular biology31(16)3312-25(2011 Aug)
Palangat M,Grass JA,Langelier MF,Coulombe B,Landick R

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POLR2B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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