gRNAs
The following polr2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the polr2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ODa48723 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001024461.3 , XM_017358972.1 , XM_017358972.2 | |
| Accession Version | NM_001024461.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-06 | |
| Organism | Danio rerio(zebrafish) | |
| Product | DNA-directed RNA polymerase II subunit RPB2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL954718.42, CABZ01048451.1, CABZ01048450.1 and CABZ01048449.1. On Nov 26, 2014 this sequence version replaced NM_001024461.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01008049.1, GDQQ01004656.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505373, SAMEA4476731 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTATGATC AAGACGAAGA TATCCAGTAT GATGAAGATG ATGATGAAAT CACCCCTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001019632.2 |
| CDS | 96..3620 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001024461.3 |
1 | ATGTATGATC AAGACGAAGA TATCCAGTAT GATGAAGATG ATGATGAAAT CACCCCTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa48723 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001024461.3 , XM_017358972.1 , XM_017358972.2 | |
| Accession Version | XM_017358972.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-23 | |
| Organism | Danio rerio(Zebrafish) | |
| Product | DNA-directed RNA polymerase II subunit RPB2 isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTATGATC AAGACGAAGA TATCCAGTAT GATGAAGATG ATGATGAAAT CACCCCTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017214461.1 |
| CDS | 86..3610 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017358972.1 |
1 | ATGTATGATC AAGACGAAGA TATCCAGTAT GATGAAGATG ATGATGAAAT CACCCCTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa48723 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001024461.3 , XM_017358972.1 , XM_017358972.2 | |
| Accession Version | XM_017358972.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-06-14 | |
| Organism | Danio rerio(zebrafish) | |
| Product | DNA-directed RNA polymerase II subunit RPB2 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTATGATC AAGACGAAGA TATCCAGTAT GATGAAGATG ATGATGAAAT CACCCCTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017214461.1 |
| CDS | 86..3610 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017358972.2 |
1 | ATGTATGATC AAGACGAAGA TATCCAGTAT GATGAAGATG ATGATGAAAT CACCCCTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Identification of 315 genes essential for early zebrafish development. |
