polr2b cDNA ORF clone, Danio rerio(zebrafish)

The following polr2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the polr2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa48723 NM_001024461.3
Latest version!
Danio rerio polymerase (RNA) II (DNA directed) polypeptide B (polr2b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa48723 XM_017358972.2
Latest version!
Danio rerio polymerase (RNA) II (DNA directed) polypeptide B (polr2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa48723 XM_017358972.1 Danio rerio polymerase (RNA) II (DNA directed) polypeptide B (polr2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa48723
    Clone ID Related Accession (Same CDS sequence) NM_001024461.3 , XM_017358972.1 , XM_017358972.2
    Accession Version NM_001024461.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-06
    Organism Danio rerio(zebrafish)
    Product DNA-directed RNA polymerase II subunit RPB2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL954718.42, CABZ01048451.1, CABZ01048450.1 and CABZ01048449.1. On Nov 26, 2014 this sequence version replaced NM_001024461.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01008049.1, GDQQ01004656.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505373, SAMEA4476731 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTATGATC AAGACGAAGA TATCCAGTAT GATGAAGATG ATGATGAAAT CACCCCTGAC 
    CTGTGGCAGG AGGCCTGCTG GATTGTTATC AGCTCTTATT TTGATGAGAA GGGTTTGGTG
    CGGCAGCAGC TGGACTCCTT CGATGAGTTC ATCCAGATGT CTGTGCAGAG GATAGTGGAG
    GACGCTCCTC CCATAGACCT GCAGGCTGAA GCCCAGCACA CATCTGGAGA GGTGGAGGAA
    CCGCCTCGTT ACCTGCTGAA GTTTGAGCAG ATCTACCTGT CCAAACCCAC GCACTGGGAA
    AGAGACGGCG CTCCGTCCCC TATGATGCCG AACGAAGCCA GACTTCGAAA TCTCACGTAT
    TCTGCTCCTC TGTATGTGGA CATCACAAAG ACCATCATAA AGGACGGGGA GGAGCAGCAG
    CAGACGCAGC ATCAGAAAAC ATTCATCGGC AAGATTCCCA TCATGCTCCG CTCCACGTAC
    TGTCTGCTGA GCGGGCTGAC GGACAGAGAT CTCTGTGAGC TGAACGAGTG TCCACTGGAC
    CCCGGAGGAT ACTTCATCAT CAACGGCTCA GAGAAGGTTC TGATTGCTCA GGAAAAGATG
    GCCACTAACA CAGTGTATGT GTTCGCCAAG AAAGACTCCA AATATGCGTA CACCGGAGAG
    TGCAGGTCCT GTCTGGAGAA CTCGTCCAGA CCCACCAGCA CCATCTGGGT CAGCATGATG
    GCCCGAGGAG GACAGGGAGT AAAGAAAAGT GCCATCGGTC AGAGGATCGT CTCCACGCTG
    CCGTATATCC GGCAGGAGGT GCCCATCATC ATCGTGTTTC GCGCTTTAGG ATTCGTGTCT
    GACAGAGACA TTCTGGAGCA CATCATCTAT GACTTCGATG ATCCGGAGAT GATGGAAATG
    GTAAAGCCGT CTCTGGATGA GGCGTTTGTG ATTCAGGAGC AGAACGTGGC TCTGAACTTC
    ATCGGCTCCA GAGGGGCCAA ACCTGGAGTC ACTAAAGAGA GGAGAATTAA ATACGCTAAA
    GAGGTTCTGC AGAAGGAGGT GCTGCCGCAT GTGGGTGTGT CAGACTTCTG CGAGACCAAG
    AAGGCTTACT TTCTAGGTTA TATGGTCCAC CGTCTGCTGC TGGCCGCTCT GGGCCGACGA
    GAGCTGGACG ACAGAGATCA TTACGGCAAC AAGAGGCTGG ATCTCGCTGG ACCGCTGCTC
    GCCTTCCTTT TCAGAGGAAT GTTCAAGAAC CTGCTGAAGG AGATCAGGAT TTACGCACAG
    AAGTTCATCG ATAGAGGGAA GGACTTCAAT CTGGAGCTGG CCATTAAAAC ACGCATCATT
    TCTGACGGAC TGAAGTATTC TCTGGCCACG GGGAACTGGG GTGACGTGAA GAAAGCCCAC
    CAGGCCAGAG CTGGAGTCTC TCAGGTGTTG AATCGGCTGA CCTTCGCCTC CACTCTCTCC
    CACCTGCGGC GTGTAAACTC TCCCATCGGC CGGGATGGTA AGCTGGCCAA ACCGCGGCAG
    CTCCACAACA CGCTGTGGGG AATGGTGTGT CCTGCAGAGA CCCCCGAGGG TCACGCTGTG
    GGTCTGGTGA AGAATCTGGC GCTCATGGCC TACATCTCTG TGGGCTCGCA GCCGTCACCC
    ATCCTGGAGT TTTTGGAGGA GTGGAGTATG GAGAATCTGG AGGAGATCTC TCCAGCTGCC
    ATCGCAGACG CCACTAAGAT CTTTGTGAAC GGCTGCTGGG TGGGCATCCA CAAAGACCCC
    GAGCAGCTGA TGAACACGCT GCGCAAGCTG AGGAGGCAGA TGGACATCAT CGTGTCTGAG
    GTCTCCATGA TCAGGGATAT CCGCGAGCGA GAGATCCGCA TCTACACTGA CGCCGGCCGC
    ATCTGCAGAC CCCTGCTGAT CGTGGAGAAA CAGAAGCTGC TGCTGAAGAG ACGACACATC
    GACCAGCTCA AGGAGAGAGA GTACAACAAC TACAGTTGGC AGGATCTGGT GGCCAGCGGT
    GTGGTGGAGT ACATCGATAC ACTGGAGGAG GAGACTGTGA TGCTGGCCAT GACCCCTGAT
    GACCTCCAGG AGAAGGGCGT GGCTTATTGC TCCACATACA CGCACTGTGA GATCCACCCG
    TCCATGATCC TGGGCGTCTG CGCATCCATC ATTCCCTTCC CTGATCACAA CCAGTCTCCC
    AGAAACACCT ACCAGTCTGC TATGGGGAAG CAGGCTATGG GAGTGTACAT CACTAATTTC
    CACGTGCGGA TGGACACGCT GGCACACGTG CTGTATTACC CACAGAAACC GCTGGTCACC
    ACACGCTCCA TGGAGTATCT ACGCTTCAGA GAGCTGCCCG CAGGAATTAA CTCCATCGTG
    GCGATCGCCT CATATACTGG ATACAACCAG GAGGATTCTG TGATCATGAA CCGCTCAGCT
    GTGGACCGCG GGTTCTTCAG GTCTGTGTTC TACCGCTCGT ACAAGGAGCA GGAGTCTAAG
    AAAGGCTTTG ACCAGGAAGA AATCTTCGAG AAACCTACGC GAGAGACCTG TCAAGGAATG
    AGACACGCCA TCTATGATAA GCTGGATGAT GACGGTCTGA TTGCTCCGGG TGTGCGTGTG
    TCCGGTGAGG ATGTGATCAT CGGTAAAACT GTGACTCTGC CAGAGAACGA TGATGAGCTG
    GACAGCACCA ACCGCCGCTA CACCAAGCGG GACTGCAGCA CGTTCCTGCG CACCAGCGAG
    ACCGGCATCG TGGACCAGGT CATGGTCACG CTTAACCAGG AGGGATACAA GTTCTGCAAA
    ATCAGGGTGC GCTCAGTTCG TATTCCACAG ATCGGGGATA AGTTTGCCAG TCGACACGGA
    CAGAAGGGAA CCTGCGGGAT CCAGTACAGA CAAGAGGATA TGCCGTTCAC CTGTGAGGGA
    ATCACTCCAG ATATCATCAT CAATCCTCAC GCCATCCCAT CCAGAATGAC AGTCGGCCAT
    TTGATCGAGT GTCTGCAGGG GAAGGTGTCT GCTAATAAAG GAGAGATCGG TGACGCCACG
    CCATTCAATG ATGCTGTCAA TGTGCAGAAG GTCTCCAACC TGCTGTCTGA ATACGGCTAC
    CATCTCAGAG GAAATGAGGT TCTGTATAAC GGCTTCACGG GTCGTAAACT GACGTCTCAG
    ATCTTCGTCG GACCCACATA TTACCAGCGT CTGAAGCACA TGGTGGACGA TAAGATCCAC
    TCCAGAGCCC GCGGGCCGGT GCAGATCCTC AACAGACAGC CCATGGAGGG GCGATCACGT
    GACGGTGGTC TGCGTTTCGG AGAGATGGAG CGTGATTGTC AGATCGCTCA CGGAGCTGCT
    CAGTTCCTGA AGGAGCGTCT GTTCGAGGCC TCGGACCCAT ATCAGGTGCA CGTGTGTAAC
    TTGTGTGGAC TCATGGCCAT CGCTAACACA CGCACACACA CCTACGAGTG CAGAGGCTGC
    CGCAACAAAA CGCAGATCTC TCTGGTGCGG ATGCCGTACG CCTGTAAACT GCTGTTCCAG
    GAGCTGATGT CCATGAGCAT CGCCCCGCGC ATGATGACCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001019632.2
    CDS96..3620
    Translation

    Target ORF information:

    RefSeq Version NM_001024461.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio polymerase (RNA) II (DNA directed) polypeptide B (polr2b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001024461.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTATGATC AAGACGAAGA TATCCAGTAT GATGAAGATG ATGATGAAAT CACCCCTGAC 
    CTGTGGCAGG AGGCCTGCTG GATTGTTATC AGCTCTTATT TTGATGAGAA GGGTTTGGTG
    CGGCAGCAGC TGGACTCCTT CGATGAGTTC ATCCAGATGT CTGTGCAGAG GATAGTGGAG
    GACGCTCCTC CCATAGACCT GCAGGCTGAA GCCCAGCACA CATCTGGAGA GGTGGAGGAA
    CCGCCTCGTT ACCTGCTGAA GTTTGAGCAG ATCTACCTGT CCAAACCCAC GCACTGGGAA
    AGAGACGGCG CTCCGTCCCC TATGATGCCG AACGAAGCCA GACTTCGAAA TCTCACGTAT
    TCTGCTCCTC TGTATGTGGA CATCACAAAG ACCATCATAA AGGACGGGGA GGAGCAGCAG
    CAGACGCAGC ATCAGAAAAC ATTCATCGGC AAGATTCCCA TCATGCTCCG CTCCACGTAC
    TGTCTGCTGA GCGGGCTGAC GGACAGAGAT CTCTGTGAGC TGAACGAGTG TCCACTGGAC
    CCCGGAGGAT ACTTCATCAT CAACGGCTCA GAGAAGGTTC TGATTGCTCA GGAAAAGATG
    GCCACTAACA CAGTGTATGT GTTCGCCAAG AAAGACTCCA AATATGCGTA CACCGGAGAG
    TGCAGGTCCT GTCTGGAGAA CTCGTCCAGA CCCACCAGCA CCATCTGGGT CAGCATGATG
    GCCCGAGGAG GACAGGGAGT AAAGAAAAGT GCCATCGGTC AGAGGATCGT CTCCACGCTG
    CCGTATATCC GGCAGGAGGT GCCCATCATC ATCGTGTTTC GCGCTTTAGG ATTCGTGTCT
    GACAGAGACA TTCTGGAGCA CATCATCTAT GACTTCGATG ATCCGGAGAT GATGGAAATG
    GTAAAGCCGT CTCTGGATGA GGCGTTTGTG ATTCAGGAGC AGAACGTGGC TCTGAACTTC
    ATCGGCTCCA GAGGGGCCAA ACCTGGAGTC ACTAAAGAGA GGAGAATTAA ATACGCTAAA
    GAGGTTCTGC AGAAGGAGGT GCTGCCGCAT GTGGGTGTGT CAGACTTCTG CGAGACCAAG
    AAGGCTTACT TTCTAGGTTA TATGGTCCAC CGTCTGCTGC TGGCCGCTCT GGGCCGACGA
    GAGCTGGACG ACAGAGATCA TTACGGCAAC AAGAGGCTGG ATCTCGCTGG ACCGCTGCTC
    GCCTTCCTTT TCAGAGGAAT GTTCAAGAAC CTGCTGAAGG AGATCAGGAT TTACGCACAG
    AAGTTCATCG ATAGAGGGAA GGACTTCAAT CTGGAGCTGG CCATTAAAAC ACGCATCATT
    TCTGACGGAC TGAAGTATTC TCTGGCCACG GGGAACTGGG GTGACGTGAA GAAAGCCCAC
    CAGGCCAGAG CTGGAGTCTC TCAGGTGTTG AATCGGCTGA CCTTCGCCTC CACTCTCTCC
    CACCTGCGGC GTGTAAACTC TCCCATCGGC CGGGATGGTA AGCTGGCCAA ACCGCGGCAG
    CTCCACAACA CGCTGTGGGG AATGGTGTGT CCTGCAGAGA CCCCCGAGGG TCACGCTGTG
    GGTCTGGTGA AGAATCTGGC GCTCATGGCC TACATCTCTG TGGGCTCGCA GCCGTCACCC
    ATCCTGGAGT TTTTGGAGGA GTGGAGTATG GAGAATCTGG AGGAGATCTC TCCAGCTGCC
    ATCGCAGACG CCACTAAGAT CTTTGTGAAC GGCTGCTGGG TGGGCATCCA CAAAGACCCC
    GAGCAGCTGA TGAACACGCT GCGCAAGCTG AGGAGGCAGA TGGACATCAT CGTGTCTGAG
    GTCTCCATGA TCAGGGATAT CCGCGAGCGA GAGATCCGCA TCTACACTGA CGCCGGCCGC
    ATCTGCAGAC CCCTGCTGAT CGTGGAGAAA CAGAAGCTGC TGCTGAAGAG ACGACACATC
    GACCAGCTCA AGGAGAGAGA GTACAACAAC TACAGTTGGC AGGATCTGGT GGCCAGCGGT
    GTGGTGGAGT ACATCGATAC ACTGGAGGAG GAGACTGTGA TGCTGGCCAT GACCCCTGAT
    GACCTCCAGG AGAAGGGCGT GGCTTATTGC TCCACATACA CGCACTGTGA GATCCACCCG
    TCCATGATCC TGGGCGTCTG CGCATCCATC ATTCCCTTCC CTGATCACAA CCAGTCTCCC
    AGAAACACCT ACCAGTCTGC TATGGGGAAG CAGGCTATGG GAGTGTACAT CACTAATTTC
    CACGTGCGGA TGGACACGCT GGCACACGTG CTGTATTACC CACAGAAACC GCTGGTCACC
    ACACGCTCCA TGGAGTATCT ACGCTTCAGA GAGCTGCCCG CAGGAATTAA CTCCATCGTG
    GCGATCGCCT CATATACTGG ATACAACCAG GAGGATTCTG TGATCATGAA CCGCTCAGCT
    GTGGACCGCG GGTTCTTCAG GTCTGTGTTC TACCGCTCGT ACAAGGAGCA GGAGTCTAAG
    AAAGGCTTTG ACCAGGAAGA AATCTTCGAG AAACCTACGC GAGAGACCTG TCAAGGAATG
    AGACACGCCA TCTATGATAA GCTGGATGAT GACGGTCTGA TTGCTCCGGG TGTGCGTGTG
    TCCGGTGAGG ATGTGATCAT CGGTAAAACT GTGACTCTGC CAGAGAACGA TGATGAGCTG
    GACAGCACCA ACCGCCGCTA CACCAAGCGG GACTGCAGCA CGTTCCTGCG CACCAGCGAG
    ACCGGCATCG TGGACCAGGT CATGGTCACG CTTAACCAGG AGGGATACAA GTTCTGCAAA
    ATCAGGGTGC GCTCAGTTCG TATTCCACAG ATCGGGGATA AGTTTGCCAG TCGACACGGA
    CAGAAGGGAA CCTGCGGGAT CCAGTACAGA CAAGAGGATA TGCCGTTCAC CTGTGAGGGA
    ATCACTCCAG ATATCATCAT CAATCCTCAC GCCATCCCAT CCAGAATGAC AGTCGGCCAT
    TTGATCGAGT GTCTGCAGGG GAAGGTGTCT GCTAATAAAG GAGAGATCGG TGACGCCACG
    CCATTCAATG ATGCTGTCAA TGTGCAGAAG GTCTCCAACC TGCTGTCTGA ATACGGCTAC
    CATCTCAGAG GAAATGAGGT TCTGTATAAC GGCTTCACGG GTCGTAAACT GACGTCTCAG
    ATCTTCGTCG GACCCACATA TTACCAGCGT CTGAAGCACA TGGTGGACGA TAAGATCCAC
    TCCAGAGCCC GCGGGCCGGT GCAGATCCTC AACAGACAGC CCATGGAGGG GCGATCACGT
    GACGGTGGTC TGCGTTTCGG AGAGATGGAG CGTGATTGTC AGATCGCTCA CGGAGCTGCT
    CAGTTCCTGA AGGAGCGTCT GTTCGAGGCC TCGGACCCAT ATCAGGTGCA CGTGTGTAAC
    TTGTGTGGAC TCATGGCCAT CGCTAACACA CGCACACACA CCTACGAGTG CAGAGGCTGC
    CGCAACAAAA CGCAGATCTC TCTGGTGCGG ATGCCGTACG CCTGTAAACT GCTGTTCCAG
    GAGCTGATGT CCATGAGCAT CGCCCCGCGC ATGATGACCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48723
    Clone ID Related Accession (Same CDS sequence) NM_001024461.3 , XM_017358972.1 , XM_017358972.2
    Accession Version XM_017358972.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product DNA-directed RNA polymerase II subunit RPB2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTATGATC AAGACGAAGA TATCCAGTAT GATGAAGATG ATGATGAAAT CACCCCTGAC 
    CTGTGGCAGG AGGCCTGCTG GATTGTTATC AGCTCTTATT TTGATGAGAA GGGTTTGGTG
    CGGCAGCAGC TGGACTCCTT CGATGAGTTC ATCCAGATGT CTGTGCAGAG GATAGTGGAG
    GACGCTCCTC CCATAGACCT GCAGGCTGAA GCCCAGCACA CATCTGGAGA GGTGGAGGAA
    CCGCCTCGTT ACCTGCTGAA GTTTGAGCAG ATCTACCTGT CCAAACCCAC GCACTGGGAA
    AGAGACGGCG CTCCGTCCCC TATGATGCCG AACGAAGCCA GACTTCGAAA TCTCACGTAT
    TCTGCTCCTC TGTATGTGGA CATCACAAAG ACCATCATAA AGGACGGGGA GGAGCAGCAG
    CAGACGCAGC ATCAGAAAAC ATTCATCGGC AAGATTCCCA TCATGCTCCG CTCCACGTAC
    TGTCTGCTGA GCGGGCTGAC GGACAGAGAT CTCTGTGAGC TGAACGAGTG TCCACTGGAC
    CCCGGAGGAT ACTTCATCAT CAACGGCTCA GAGAAGGTTC TGATTGCTCA GGAAAAGATG
    GCCACTAACA CAGTGTATGT GTTCGCCAAG AAAGACTCCA AATATGCGTA CACCGGAGAG
    TGCAGGTCCT GTCTGGAGAA CTCGTCCAGA CCCACCAGCA CCATCTGGGT CAGCATGATG
    GCCCGAGGAG GACAGGGAGT AAAGAAAAGT GCCATCGGTC AGAGGATCGT CTCCACGCTG
    CCGTATATCC GGCAGGAGGT GCCCATCATC ATCGTGTTTC GCGCTTTAGG ATTCGTGTCT
    GACAGAGACA TTCTGGAGCA CATCATCTAT GACTTCGATG ATCCGGAGAT GATGGAAATG
    GTAAAGCCGT CTCTGGATGA GGCGTTTGTG ATTCAGGAGC AGAACGTGGC TCTGAACTTC
    ATCGGCTCCA GAGGGGCCAA ACCTGGAGTC ACTAAAGAGA GGAGAATTAA ATACGCTAAA
    GAGGTTCTGC AGAAGGAGGT GCTGCCGCAT GTGGGTGTGT CAGACTTCTG CGAGACCAAG
    AAGGCTTACT TTCTAGGTTA TATGGTCCAC CGTCTGCTGC TGGCCGCTCT GGGCCGACGA
    GAGCTGGACG ACAGAGATCA TTACGGCAAC AAGAGGCTGG ATCTCGCTGG ACCGCTGCTC
    GCCTTCCTTT TCAGAGGAAT GTTCAAGAAC CTGCTGAAGG AGATCAGGAT TTACGCACAG
    AAGTTCATCG ATAGAGGGAA GGACTTCAAT CTGGAGCTGG CCATTAAAAC ACGCATCATT
    TCTGACGGAC TGAAGTATTC TCTGGCCACG GGGAACTGGG GTGACGTGAA GAAAGCCCAC
    CAGGCCAGAG CTGGAGTCTC TCAGGTGTTG AATCGGCTGA CCTTCGCCTC CACTCTCTCC
    CACCTGCGGC GTGTAAACTC TCCCATCGGC CGGGATGGTA AGCTGGCCAA ACCGCGGCAG
    CTCCACAACA CGCTGTGGGG AATGGTGTGT CCTGCAGAGA CCCCCGAGGG TCACGCTGTG
    GGTCTGGTGA AGAATCTGGC GCTCATGGCC TACATCTCTG TGGGCTCGCA GCCGTCACCC
    ATCCTGGAGT TTTTGGAGGA GTGGAGTATG GAGAATCTGG AGGAGATCTC TCCAGCTGCC
    ATCGCAGACG CCACTAAGAT CTTTGTGAAC GGCTGCTGGG TGGGCATCCA CAAAGACCCC
    GAGCAGCTGA TGAACACGCT GCGCAAGCTG AGGAGGCAGA TGGACATCAT CGTGTCTGAG
    GTCTCCATGA TCAGGGATAT CCGCGAGCGA GAGATCCGCA TCTACACTGA CGCCGGCCGC
    ATCTGCAGAC CCCTGCTGAT CGTGGAGAAA CAGAAGCTGC TGCTGAAGAG ACGACACATC
    GACCAGCTCA AGGAGAGAGA GTACAACAAC TACAGTTGGC AGGATCTGGT GGCCAGCGGT
    GTGGTGGAGT ACATCGATAC ACTGGAGGAG GAGACTGTGA TGCTGGCCAT GACCCCTGAT
    GACCTCCAGG AGAAGGGCGT GGCTTATTGC TCCACATACA CGCACTGTGA GATCCACCCG
    TCCATGATCC TGGGCGTCTG CGCATCCATC ATTCCCTTCC CTGATCACAA CCAGTCTCCC
    AGAAACACCT ACCAGTCTGC TATGGGGAAG CAGGCTATGG GAGTGTACAT CACTAATTTC
    CACGTGCGGA TGGACACGCT GGCACACGTG CTGTATTACC CACAGAAACC GCTGGTCACC
    ACACGCTCCA TGGAGTATCT ACGCTTCAGA GAGCTGCCCG CAGGAATTAA CTCCATCGTG
    GCGATCGCCT CATATACTGG ATACAACCAG GAGGATTCTG TGATCATGAA CCGCTCAGCT
    GTGGACCGCG GGTTCTTCAG GTCTGTGTTC TACCGCTCGT ACAAGGAGCA GGAGTCTAAG
    AAAGGCTTTG ACCAGGAAGA AATCTTCGAG AAACCTACGC GAGAGACCTG TCAAGGAATG
    AGACACGCCA TCTATGATAA GCTGGATGAT GACGGTCTGA TTGCTCCGGG TGTGCGTGTG
    TCCGGTGAGG ATGTGATCAT CGGTAAAACT GTGACTCTGC CAGAGAACGA TGATGAGCTG
    GACAGCACCA ACCGCCGCTA CACCAAGCGG GACTGCAGCA CGTTCCTGCG CACCAGCGAG
    ACCGGCATCG TGGACCAGGT CATGGTCACG CTTAACCAGG AGGGATACAA GTTCTGCAAA
    ATCAGGGTGC GCTCAGTTCG TATTCCACAG ATCGGGGATA AGTTTGCCAG TCGACACGGA
    CAGAAGGGAA CCTGCGGGAT CCAGTACAGA CAAGAGGATA TGCCGTTCAC CTGTGAGGGA
    ATCACTCCAG ATATCATCAT CAATCCTCAC GCCATCCCAT CCAGAATGAC AGTCGGCCAT
    TTGATCGAGT GTCTGCAGGG GAAGGTGTCT GCTAATAAAG GAGAGATCGG TGACGCCACG
    CCATTCAATG ATGCTGTCAA TGTGCAGAAG GTCTCCAACC TGCTGTCTGA ATACGGCTAC
    CATCTCAGAG GAAATGAGGT TCTGTATAAC GGCTTCACGG GTCGTAAACT GACGTCTCAG
    ATCTTCGTCG GACCCACATA TTACCAGCGT CTGAAGCACA TGGTGGACGA TAAGATCCAC
    TCCAGAGCCC GCGGGCCGGT GCAGATCCTC AACAGACAGC CCATGGAGGG GCGATCACGT
    GACGGTGGTC TGCGTTTCGG AGAGATGGAG CGTGATTGTC AGATCGCTCA CGGAGCTGCT
    CAGTTCCTGA AGGAGCGTCT GTTCGAGGCC TCGGACCCAT ATCAGGTGCA CGTGTGTAAC
    TTGTGTGGAC TCATGGCCAT CGCTAACACA CGCACACACA CCTACGAGTG CAGAGGCTGC
    CGCAACAAAA CGCAGATCTC TCTGGTGCGG ATGCCGTACG CCTGTAAACT GCTGTTCCAG
    GAGCTGATGT CCATGAGCAT CGCCCCGCGC ATGATGACCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214461.1
    CDS86..3610
    Translation

    Target ORF information:

    RefSeq Version XM_017358972.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio polymerase (RNA) II (DNA directed) polypeptide B (polr2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358972.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTATGATC AAGACGAAGA TATCCAGTAT GATGAAGATG ATGATGAAAT CACCCCTGAC 
    CTGTGGCAGG AGGCCTGCTG GATTGTTATC AGCTCTTATT TTGATGAGAA GGGTTTGGTG
    CGGCAGCAGC TGGACTCCTT CGATGAGTTC ATCCAGATGT CTGTGCAGAG GATAGTGGAG
    GACGCTCCTC CCATAGACCT GCAGGCTGAA GCCCAGCACA CATCTGGAGA GGTGGAGGAA
    CCGCCTCGTT ACCTGCTGAA GTTTGAGCAG ATCTACCTGT CCAAACCCAC GCACTGGGAA
    AGAGACGGCG CTCCGTCCCC TATGATGCCG AACGAAGCCA GACTTCGAAA TCTCACGTAT
    TCTGCTCCTC TGTATGTGGA CATCACAAAG ACCATCATAA AGGACGGGGA GGAGCAGCAG
    CAGACGCAGC ATCAGAAAAC ATTCATCGGC AAGATTCCCA TCATGCTCCG CTCCACGTAC
    TGTCTGCTGA GCGGGCTGAC GGACAGAGAT CTCTGTGAGC TGAACGAGTG TCCACTGGAC
    CCCGGAGGAT ACTTCATCAT CAACGGCTCA GAGAAGGTTC TGATTGCTCA GGAAAAGATG
    GCCACTAACA CAGTGTATGT GTTCGCCAAG AAAGACTCCA AATATGCGTA CACCGGAGAG
    TGCAGGTCCT GTCTGGAGAA CTCGTCCAGA CCCACCAGCA CCATCTGGGT CAGCATGATG
    GCCCGAGGAG GACAGGGAGT AAAGAAAAGT GCCATCGGTC AGAGGATCGT CTCCACGCTG
    CCGTATATCC GGCAGGAGGT GCCCATCATC ATCGTGTTTC GCGCTTTAGG ATTCGTGTCT
    GACAGAGACA TTCTGGAGCA CATCATCTAT GACTTCGATG ATCCGGAGAT GATGGAAATG
    GTAAAGCCGT CTCTGGATGA GGCGTTTGTG ATTCAGGAGC AGAACGTGGC TCTGAACTTC
    ATCGGCTCCA GAGGGGCCAA ACCTGGAGTC ACTAAAGAGA GGAGAATTAA ATACGCTAAA
    GAGGTTCTGC AGAAGGAGGT GCTGCCGCAT GTGGGTGTGT CAGACTTCTG CGAGACCAAG
    AAGGCTTACT TTCTAGGTTA TATGGTCCAC CGTCTGCTGC TGGCCGCTCT GGGCCGACGA
    GAGCTGGACG ACAGAGATCA TTACGGCAAC AAGAGGCTGG ATCTCGCTGG ACCGCTGCTC
    GCCTTCCTTT TCAGAGGAAT GTTCAAGAAC CTGCTGAAGG AGATCAGGAT TTACGCACAG
    AAGTTCATCG ATAGAGGGAA GGACTTCAAT CTGGAGCTGG CCATTAAAAC ACGCATCATT
    TCTGACGGAC TGAAGTATTC TCTGGCCACG GGGAACTGGG GTGACGTGAA GAAAGCCCAC
    CAGGCCAGAG CTGGAGTCTC TCAGGTGTTG AATCGGCTGA CCTTCGCCTC CACTCTCTCC
    CACCTGCGGC GTGTAAACTC TCCCATCGGC CGGGATGGTA AGCTGGCCAA ACCGCGGCAG
    CTCCACAACA CGCTGTGGGG AATGGTGTGT CCTGCAGAGA CCCCCGAGGG TCACGCTGTG
    GGTCTGGTGA AGAATCTGGC GCTCATGGCC TACATCTCTG TGGGCTCGCA GCCGTCACCC
    ATCCTGGAGT TTTTGGAGGA GTGGAGTATG GAGAATCTGG AGGAGATCTC TCCAGCTGCC
    ATCGCAGACG CCACTAAGAT CTTTGTGAAC GGCTGCTGGG TGGGCATCCA CAAAGACCCC
    GAGCAGCTGA TGAACACGCT GCGCAAGCTG AGGAGGCAGA TGGACATCAT CGTGTCTGAG
    GTCTCCATGA TCAGGGATAT CCGCGAGCGA GAGATCCGCA TCTACACTGA CGCCGGCCGC
    ATCTGCAGAC CCCTGCTGAT CGTGGAGAAA CAGAAGCTGC TGCTGAAGAG ACGACACATC
    GACCAGCTCA AGGAGAGAGA GTACAACAAC TACAGTTGGC AGGATCTGGT GGCCAGCGGT
    GTGGTGGAGT ACATCGATAC ACTGGAGGAG GAGACTGTGA TGCTGGCCAT GACCCCTGAT
    GACCTCCAGG AGAAGGGCGT GGCTTATTGC TCCACATACA CGCACTGTGA GATCCACCCG
    TCCATGATCC TGGGCGTCTG CGCATCCATC ATTCCCTTCC CTGATCACAA CCAGTCTCCC
    AGAAACACCT ACCAGTCTGC TATGGGGAAG CAGGCTATGG GAGTGTACAT CACTAATTTC
    CACGTGCGGA TGGACACGCT GGCACACGTG CTGTATTACC CACAGAAACC GCTGGTCACC
    ACACGCTCCA TGGAGTATCT ACGCTTCAGA GAGCTGCCCG CAGGAATTAA CTCCATCGTG
    GCGATCGCCT CATATACTGG ATACAACCAG GAGGATTCTG TGATCATGAA CCGCTCAGCT
    GTGGACCGCG GGTTCTTCAG GTCTGTGTTC TACCGCTCGT ACAAGGAGCA GGAGTCTAAG
    AAAGGCTTTG ACCAGGAAGA AATCTTCGAG AAACCTACGC GAGAGACCTG TCAAGGAATG
    AGACACGCCA TCTATGATAA GCTGGATGAT GACGGTCTGA TTGCTCCGGG TGTGCGTGTG
    TCCGGTGAGG ATGTGATCAT CGGTAAAACT GTGACTCTGC CAGAGAACGA TGATGAGCTG
    GACAGCACCA ACCGCCGCTA CACCAAGCGG GACTGCAGCA CGTTCCTGCG CACCAGCGAG
    ACCGGCATCG TGGACCAGGT CATGGTCACG CTTAACCAGG AGGGATACAA GTTCTGCAAA
    ATCAGGGTGC GCTCAGTTCG TATTCCACAG ATCGGGGATA AGTTTGCCAG TCGACACGGA
    CAGAAGGGAA CCTGCGGGAT CCAGTACAGA CAAGAGGATA TGCCGTTCAC CTGTGAGGGA
    ATCACTCCAG ATATCATCAT CAATCCTCAC GCCATCCCAT CCAGAATGAC AGTCGGCCAT
    TTGATCGAGT GTCTGCAGGG GAAGGTGTCT GCTAATAAAG GAGAGATCGG TGACGCCACG
    CCATTCAATG ATGCTGTCAA TGTGCAGAAG GTCTCCAACC TGCTGTCTGA ATACGGCTAC
    CATCTCAGAG GAAATGAGGT TCTGTATAAC GGCTTCACGG GTCGTAAACT GACGTCTCAG
    ATCTTCGTCG GACCCACATA TTACCAGCGT CTGAAGCACA TGGTGGACGA TAAGATCCAC
    TCCAGAGCCC GCGGGCCGGT GCAGATCCTC AACAGACAGC CCATGGAGGG GCGATCACGT
    GACGGTGGTC TGCGTTTCGG AGAGATGGAG CGTGATTGTC AGATCGCTCA CGGAGCTGCT
    CAGTTCCTGA AGGAGCGTCT GTTCGAGGCC TCGGACCCAT ATCAGGTGCA CGTGTGTAAC
    TTGTGTGGAC TCATGGCCAT CGCTAACACA CGCACACACA CCTACGAGTG CAGAGGCTGC
    CGCAACAAAA CGCAGATCTC TCTGGTGCGG ATGCCGTACG CCTGTAAACT GCTGTTCCAG
    GAGCTGATGT CCATGAGCAT CGCCCCGCGC ATGATGACCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48723
    Clone ID Related Accession (Same CDS sequence) NM_001024461.3 , XM_017358972.1 , XM_017358972.2
    Accession Version XM_017358972.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product DNA-directed RNA polymerase II subunit RPB2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTATGATC AAGACGAAGA TATCCAGTAT GATGAAGATG ATGATGAAAT CACCCCTGAC 
    CTGTGGCAGG AGGCCTGCTG GATTGTTATC AGCTCTTATT TTGATGAGAA GGGTTTGGTG
    CGGCAGCAGC TGGACTCCTT CGATGAGTTC ATCCAGATGT CTGTGCAGAG GATAGTGGAG
    GACGCTCCTC CCATAGACCT GCAGGCTGAA GCCCAGCACA CATCTGGAGA GGTGGAGGAA
    CCGCCTCGTT ACCTGCTGAA GTTTGAGCAG ATCTACCTGT CCAAACCCAC GCACTGGGAA
    AGAGACGGCG CTCCGTCCCC TATGATGCCG AACGAAGCCA GACTTCGAAA TCTCACGTAT
    TCTGCTCCTC TGTATGTGGA CATCACAAAG ACCATCATAA AGGACGGGGA GGAGCAGCAG
    CAGACGCAGC ATCAGAAAAC ATTCATCGGC AAGATTCCCA TCATGCTCCG CTCCACGTAC
    TGTCTGCTGA GCGGGCTGAC GGACAGAGAT CTCTGTGAGC TGAACGAGTG TCCACTGGAC
    CCCGGAGGAT ACTTCATCAT CAACGGCTCA GAGAAGGTTC TGATTGCTCA GGAAAAGATG
    GCCACTAACA CAGTGTATGT GTTCGCCAAG AAAGACTCCA AATATGCGTA CACCGGAGAG
    TGCAGGTCCT GTCTGGAGAA CTCGTCCAGA CCCACCAGCA CCATCTGGGT CAGCATGATG
    GCCCGAGGAG GACAGGGAGT AAAGAAAAGT GCCATCGGTC AGAGGATCGT CTCCACGCTG
    CCGTATATCC GGCAGGAGGT GCCCATCATC ATCGTGTTTC GCGCTTTAGG ATTCGTGTCT
    GACAGAGACA TTCTGGAGCA CATCATCTAT GACTTCGATG ATCCGGAGAT GATGGAAATG
    GTAAAGCCGT CTCTGGATGA GGCGTTTGTG ATTCAGGAGC AGAACGTGGC TCTGAACTTC
    ATCGGCTCCA GAGGGGCCAA ACCTGGAGTC ACTAAAGAGA GGAGAATTAA ATACGCTAAA
    GAGGTTCTGC AGAAGGAGGT GCTGCCGCAT GTGGGTGTGT CAGACTTCTG CGAGACCAAG
    AAGGCTTACT TTCTAGGTTA TATGGTCCAC CGTCTGCTGC TGGCCGCTCT GGGCCGACGA
    GAGCTGGACG ACAGAGATCA TTACGGCAAC AAGAGGCTGG ATCTCGCTGG ACCGCTGCTC
    GCCTTCCTTT TCAGAGGAAT GTTCAAGAAC CTGCTGAAGG AGATCAGGAT TTACGCACAG
    AAGTTCATCG ATAGAGGGAA GGACTTCAAT CTGGAGCTGG CCATTAAAAC ACGCATCATT
    TCTGACGGAC TGAAGTATTC TCTGGCCACG GGGAACTGGG GTGACGTGAA GAAAGCCCAC
    CAGGCCAGAG CTGGAGTCTC TCAGGTGTTG AATCGGCTGA CCTTCGCCTC CACTCTCTCC
    CACCTGCGGC GTGTAAACTC TCCCATCGGC CGGGATGGTA AGCTGGCCAA ACCGCGGCAG
    CTCCACAACA CGCTGTGGGG AATGGTGTGT CCTGCAGAGA CCCCCGAGGG TCACGCTGTG
    GGTCTGGTGA AGAATCTGGC GCTCATGGCC TACATCTCTG TGGGCTCGCA GCCGTCACCC
    ATCCTGGAGT TTTTGGAGGA GTGGAGTATG GAGAATCTGG AGGAGATCTC TCCAGCTGCC
    ATCGCAGACG CCACTAAGAT CTTTGTGAAC GGCTGCTGGG TGGGCATCCA CAAAGACCCC
    GAGCAGCTGA TGAACACGCT GCGCAAGCTG AGGAGGCAGA TGGACATCAT CGTGTCTGAG
    GTCTCCATGA TCAGGGATAT CCGCGAGCGA GAGATCCGCA TCTACACTGA CGCCGGCCGC
    ATCTGCAGAC CCCTGCTGAT CGTGGAGAAA CAGAAGCTGC TGCTGAAGAG ACGACACATC
    GACCAGCTCA AGGAGAGAGA GTACAACAAC TACAGTTGGC AGGATCTGGT GGCCAGCGGT
    GTGGTGGAGT ACATCGATAC ACTGGAGGAG GAGACTGTGA TGCTGGCCAT GACCCCTGAT
    GACCTCCAGG AGAAGGGCGT GGCTTATTGC TCCACATACA CGCACTGTGA GATCCACCCG
    TCCATGATCC TGGGCGTCTG CGCATCCATC ATTCCCTTCC CTGATCACAA CCAGTCTCCC
    AGAAACACCT ACCAGTCTGC TATGGGGAAG CAGGCTATGG GAGTGTACAT CACTAATTTC
    CACGTGCGGA TGGACACGCT GGCACACGTG CTGTATTACC CACAGAAACC GCTGGTCACC
    ACACGCTCCA TGGAGTATCT ACGCTTCAGA GAGCTGCCCG CAGGAATTAA CTCCATCGTG
    GCGATCGCCT CATATACTGG ATACAACCAG GAGGATTCTG TGATCATGAA CCGCTCAGCT
    GTGGACCGCG GGTTCTTCAG GTCTGTGTTC TACCGCTCGT ACAAGGAGCA GGAGTCTAAG
    AAAGGCTTTG ACCAGGAAGA AATCTTCGAG AAACCTACGC GAGAGACCTG TCAAGGAATG
    AGACACGCCA TCTATGATAA GCTGGATGAT GACGGTCTGA TTGCTCCGGG TGTGCGTGTG
    TCCGGTGAGG ATGTGATCAT CGGTAAAACT GTGACTCTGC CAGAGAACGA TGATGAGCTG
    GACAGCACCA ACCGCCGCTA CACCAAGCGG GACTGCAGCA CGTTCCTGCG CACCAGCGAG
    ACCGGCATCG TGGACCAGGT CATGGTCACG CTTAACCAGG AGGGATACAA GTTCTGCAAA
    ATCAGGGTGC GCTCAGTTCG TATTCCACAG ATCGGGGATA AGTTTGCCAG TCGACACGGA
    CAGAAGGGAA CCTGCGGGAT CCAGTACAGA CAAGAGGATA TGCCGTTCAC CTGTGAGGGA
    ATCACTCCAG ATATCATCAT CAATCCTCAC GCCATCCCAT CCAGAATGAC AGTCGGCCAT
    TTGATCGAGT GTCTGCAGGG GAAGGTGTCT GCTAATAAAG GAGAGATCGG TGACGCCACG
    CCATTCAATG ATGCTGTCAA TGTGCAGAAG GTCTCCAACC TGCTGTCTGA ATACGGCTAC
    CATCTCAGAG GAAATGAGGT TCTGTATAAC GGCTTCACGG GTCGTAAACT GACGTCTCAG
    ATCTTCGTCG GACCCACATA TTACCAGCGT CTGAAGCACA TGGTGGACGA TAAGATCCAC
    TCCAGAGCCC GCGGGCCGGT GCAGATCCTC AACAGACAGC CCATGGAGGG GCGATCACGT
    GACGGTGGTC TGCGTTTCGG AGAGATGGAG CGTGATTGTC AGATCGCTCA CGGAGCTGCT
    CAGTTCCTGA AGGAGCGTCT GTTCGAGGCC TCGGACCCAT ATCAGGTGCA CGTGTGTAAC
    TTGTGTGGAC TCATGGCCAT CGCTAACACA CGCACACACA CCTACGAGTG CAGAGGCTGC
    CGCAACAAAA CGCAGATCTC TCTGGTGCGG ATGCCGTACG CCTGTAAACT GCTGTTCCAG
    GAGCTGATGT CCATGAGCAT CGCCCCGCGC ATGATGACCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214461.1
    CDS86..3610
    Translation

    Target ORF information:

    RefSeq Version XM_017358972.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio polymerase (RNA) II (DNA directed) polypeptide B (polr2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358972.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTATGATC AAGACGAAGA TATCCAGTAT GATGAAGATG ATGATGAAAT CACCCCTGAC 
    CTGTGGCAGG AGGCCTGCTG GATTGTTATC AGCTCTTATT TTGATGAGAA GGGTTTGGTG
    CGGCAGCAGC TGGACTCCTT CGATGAGTTC ATCCAGATGT CTGTGCAGAG GATAGTGGAG
    GACGCTCCTC CCATAGACCT GCAGGCTGAA GCCCAGCACA CATCTGGAGA GGTGGAGGAA
    CCGCCTCGTT ACCTGCTGAA GTTTGAGCAG ATCTACCTGT CCAAACCCAC GCACTGGGAA
    AGAGACGGCG CTCCGTCCCC TATGATGCCG AACGAAGCCA GACTTCGAAA TCTCACGTAT
    TCTGCTCCTC TGTATGTGGA CATCACAAAG ACCATCATAA AGGACGGGGA GGAGCAGCAG
    CAGACGCAGC ATCAGAAAAC ATTCATCGGC AAGATTCCCA TCATGCTCCG CTCCACGTAC
    TGTCTGCTGA GCGGGCTGAC GGACAGAGAT CTCTGTGAGC TGAACGAGTG TCCACTGGAC
    CCCGGAGGAT ACTTCATCAT CAACGGCTCA GAGAAGGTTC TGATTGCTCA GGAAAAGATG
    GCCACTAACA CAGTGTATGT GTTCGCCAAG AAAGACTCCA AATATGCGTA CACCGGAGAG
    TGCAGGTCCT GTCTGGAGAA CTCGTCCAGA CCCACCAGCA CCATCTGGGT CAGCATGATG
    GCCCGAGGAG GACAGGGAGT AAAGAAAAGT GCCATCGGTC AGAGGATCGT CTCCACGCTG
    CCGTATATCC GGCAGGAGGT GCCCATCATC ATCGTGTTTC GCGCTTTAGG ATTCGTGTCT
    GACAGAGACA TTCTGGAGCA CATCATCTAT GACTTCGATG ATCCGGAGAT GATGGAAATG
    GTAAAGCCGT CTCTGGATGA GGCGTTTGTG ATTCAGGAGC AGAACGTGGC TCTGAACTTC
    ATCGGCTCCA GAGGGGCCAA ACCTGGAGTC ACTAAAGAGA GGAGAATTAA ATACGCTAAA
    GAGGTTCTGC AGAAGGAGGT GCTGCCGCAT GTGGGTGTGT CAGACTTCTG CGAGACCAAG
    AAGGCTTACT TTCTAGGTTA TATGGTCCAC CGTCTGCTGC TGGCCGCTCT GGGCCGACGA
    GAGCTGGACG ACAGAGATCA TTACGGCAAC AAGAGGCTGG ATCTCGCTGG ACCGCTGCTC
    GCCTTCCTTT TCAGAGGAAT GTTCAAGAAC CTGCTGAAGG AGATCAGGAT TTACGCACAG
    AAGTTCATCG ATAGAGGGAA GGACTTCAAT CTGGAGCTGG CCATTAAAAC ACGCATCATT
    TCTGACGGAC TGAAGTATTC TCTGGCCACG GGGAACTGGG GTGACGTGAA GAAAGCCCAC
    CAGGCCAGAG CTGGAGTCTC TCAGGTGTTG AATCGGCTGA CCTTCGCCTC CACTCTCTCC
    CACCTGCGGC GTGTAAACTC TCCCATCGGC CGGGATGGTA AGCTGGCCAA ACCGCGGCAG
    CTCCACAACA CGCTGTGGGG AATGGTGTGT CCTGCAGAGA CCCCCGAGGG TCACGCTGTG
    GGTCTGGTGA AGAATCTGGC GCTCATGGCC TACATCTCTG TGGGCTCGCA GCCGTCACCC
    ATCCTGGAGT TTTTGGAGGA GTGGAGTATG GAGAATCTGG AGGAGATCTC TCCAGCTGCC
    ATCGCAGACG CCACTAAGAT CTTTGTGAAC GGCTGCTGGG TGGGCATCCA CAAAGACCCC
    GAGCAGCTGA TGAACACGCT GCGCAAGCTG AGGAGGCAGA TGGACATCAT CGTGTCTGAG
    GTCTCCATGA TCAGGGATAT CCGCGAGCGA GAGATCCGCA TCTACACTGA CGCCGGCCGC
    ATCTGCAGAC CCCTGCTGAT CGTGGAGAAA CAGAAGCTGC TGCTGAAGAG ACGACACATC
    GACCAGCTCA AGGAGAGAGA GTACAACAAC TACAGTTGGC AGGATCTGGT GGCCAGCGGT
    GTGGTGGAGT ACATCGATAC ACTGGAGGAG GAGACTGTGA TGCTGGCCAT GACCCCTGAT
    GACCTCCAGG AGAAGGGCGT GGCTTATTGC TCCACATACA CGCACTGTGA GATCCACCCG
    TCCATGATCC TGGGCGTCTG CGCATCCATC ATTCCCTTCC CTGATCACAA CCAGTCTCCC
    AGAAACACCT ACCAGTCTGC TATGGGGAAG CAGGCTATGG GAGTGTACAT CACTAATTTC
    CACGTGCGGA TGGACACGCT GGCACACGTG CTGTATTACC CACAGAAACC GCTGGTCACC
    ACACGCTCCA TGGAGTATCT ACGCTTCAGA GAGCTGCCCG CAGGAATTAA CTCCATCGTG
    GCGATCGCCT CATATACTGG ATACAACCAG GAGGATTCTG TGATCATGAA CCGCTCAGCT
    GTGGACCGCG GGTTCTTCAG GTCTGTGTTC TACCGCTCGT ACAAGGAGCA GGAGTCTAAG
    AAAGGCTTTG ACCAGGAAGA AATCTTCGAG AAACCTACGC GAGAGACCTG TCAAGGAATG
    AGACACGCCA TCTATGATAA GCTGGATGAT GACGGTCTGA TTGCTCCGGG TGTGCGTGTG
    TCCGGTGAGG ATGTGATCAT CGGTAAAACT GTGACTCTGC CAGAGAACGA TGATGAGCTG
    GACAGCACCA ACCGCCGCTA CACCAAGCGG GACTGCAGCA CGTTCCTGCG CACCAGCGAG
    ACCGGCATCG TGGACCAGGT CATGGTCACG CTTAACCAGG AGGGATACAA GTTCTGCAAA
    ATCAGGGTGC GCTCAGTTCG TATTCCACAG ATCGGGGATA AGTTTGCCAG TCGACACGGA
    CAGAAGGGAA CCTGCGGGAT CCAGTACAGA CAAGAGGATA TGCCGTTCAC CTGTGAGGGA
    ATCACTCCAG ATATCATCAT CAATCCTCAC GCCATCCCAT CCAGAATGAC AGTCGGCCAT
    TTGATCGAGT GTCTGCAGGG GAAGGTGTCT GCTAATAAAG GAGAGATCGG TGACGCCACG
    CCATTCAATG ATGCTGTCAA TGTGCAGAAG GTCTCCAACC TGCTGTCTGA ATACGGCTAC
    CATCTCAGAG GAAATGAGGT TCTGTATAAC GGCTTCACGG GTCGTAAACT GACGTCTCAG
    ATCTTCGTCG GACCCACATA TTACCAGCGT CTGAAGCACA TGGTGGACGA TAAGATCCAC
    TCCAGAGCCC GCGGGCCGGT GCAGATCCTC AACAGACAGC CCATGGAGGG GCGATCACGT
    GACGGTGGTC TGCGTTTCGG AGAGATGGAG CGTGATTGTC AGATCGCTCA CGGAGCTGCT
    CAGTTCCTGA AGGAGCGTCT GTTCGAGGCC TCGGACCCAT ATCAGGTGCA CGTGTGTAAC
    TTGTGTGGAC TCATGGCCAT CGCTAACACA CGCACACACA CCTACGAGTG CAGAGGCTGC
    CGCAACAAAA CGCAGATCTC TCTGGTGCGG ATGCCGTACG CCTGTAAACT GCTGTTCCAG
    GAGCTGATGT CCATGAGCAT CGCCCCGCGC ATGATGACCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.