Mmel1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mmel1
Entrez Gene ID	27390
Full Name	membrane metallo-endopeptidase-like 1
Synonyms	Mell1,NEP2,NEPII,Nl1,SEP
General protein information	Preferred Names membrane metallo-endopeptidase-like 1 Names membrane metallo-endopeptidase-like 1 NEP2(m) NEPLP alpha NEPLP beta NEPLP gamma NL-1 NL2 mel transforming oncogene-like 1 neprilysin 2 neprilysin II neprilysin-like 1 neprilysin-like peptidase soluble secreted endopeptidase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_013783.2	NP_038811.2	membrane metallo-endopeptidase-like 1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MMEL1	XP_001233077.3
Danio rerio (zebrafish)	mmel1	XP_689191.3
Canis lupus familiaris (dog)	MMEL1	XP_005620485.1
Mus musculus (house mouse)	Mmel1	NP_038811.2
Xenopus tropicalis (tropical clawed frog)	mmel1	NP_001120567.1
Homo sapiens (human)	MMEL1	NP_258428.2
Drosophila melanogaster (fruit fly)	Nep4	NP_650904.3
Pan troglodytes (chimpanzee)	MMEL1	XP_001150860.1
Bos taurus (cattle)	MMEL1	NP_001180065.1
Rattus norvegicus (Norway rat)	Mmel1	NP_001101467.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003106	XP_312790.4
Caenorhabditis elegans (roundworm)	nep-2	NP_494343.2

Gene Ontology
Bibliography

Related articles in PubMed

Sustained Activation of Rho GTPases Promotes a Synthetic Pulmonary Artery Smooth Muscle Cell Phenotype in Neprilysin Null Mice.
Karoor V, Fini MA, Loomis Z, Sullivan T, Hersh LB, Gerasimovskaya E, Irwin D, Dempsey EC
Arteriosclerosis, thrombosis, and vascular biology38(1)154-163(2018 Jan)

Protection against vascular leak in neprilysin transgenic mice with complex?overexpression?pattern.
Wick MJ, Loomis ZL, Harral JW, Le M, Wehling CA, Miller YE, Dempsey EC
Transgenic research25(6)773-784(2016 12)

Accumulation of murine amyloid-? mimics early Alzheimer's disease.
Krohn M, Bracke A, Avchalumov Y, Schumacher T, Hofrichter J, Paarmann K, Fr?hlich C, Lange C, Br?ning T, von Bohlen Und Halbach O, Pahnke J
Brain : a journal of neurology138(Pt 8)2370-82(2015 Aug)

Cortical fast-spiking parvalbumin interneurons enwrapped in the perineuronal net express the metallopeptidases Adamts8, Adamts15 and Neprilysin.
Rossier J, Bernard A, Cabungcal JH, Perrenoud Q, Savoye A, Gallopin T, Hawrylycz M, Cu?nod M, Do K, Urban A, Lein ES
Molecular psychiatry20(2)154-61(2015 Feb)

Neprilysin-2 is an important ?-amyloid degrading enzyme.
Hafez D, Huang JY, Huynh AM, Valtierra S, Rockenstein E, Bruno AM, Lu B, DesGroseillers L, Masliah E, Marr RA
The American journal of pathology178(1)306-12(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These studies help to characterize our complex transgenic model of NEP overexpression and further demonstrate NEP's protective effects against plasma extravasation.
Title: Protection against vascular leak in neprilysin transgenic mice with complex?overexpression?pattern.

neprilysin x ABCC1 double-deficient mice present a new model for early effects of amyloid-beta-related mild cognitive impairment that allows investigations without artificial overexpression of inherited Alzheimer's disease genes
Title: Accumulation of murine amyloid-? mimics early Alzheimer's disease.

This study demonstrated that Cortical fast-spiking parvalbumin interneurons enwrapped in the perineuronal net express the Neprilysin in mice.
Title: Cortical fast-spiking parvalbumin interneurons enwrapped in the perineuronal net express the metallopeptidases Adamts8, Adamts15 and Neprilysin.

Data show that intranasal delivery of drugs can be used to model Alzheimer disease and suggest that other phosphoramidon-sensitive peptidases are degrading amyloid beta in neprilysin/NEP2-deficient mice.
Title: Intranasal phosphoramidon increases beta-amyloid levels in wild-type and NEP/NEP2-deficient mice.

In NEP/NEP2 double-knockout mice, Abeta levels were marginally increased ( approximately 1.5- to 2-fold), compared with NEP(-/-)/NEP2(+/+) controls.
Title: Neprilysin-2 is an important ?-amyloid degrading enzyme.

The following Mmel1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mmel1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu09962	NM_013783.2 Latest version!	Mus musculus membrane metallo-endopeptidase-like 1 (Mmel1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mmel1 ( NM_013783.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038811.2 Mus musculus membrane metallo-endopeptidase-like 1 (Mmel1), mRNA.

Price & Availability↑

CloneID	OMu09962
Clone ID Related Accession (Same CDS sequence)	NM_013783.2
Accession Version	NM_013783.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Mus musculus(house mouse)
Product	membrane metallo-endopeptidase-like 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF176569.1, BY105444.1, AF302076.1 and AF157105.1. On Oct 31, 2007 this sequence version replaced NM_013783.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF176569.1, AF157105.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGAGA GAGCAGGCTG GTGTCGGAAG AAGTCCCCAG GCTTCGTGGA GTATGGGCTG 
ATGGTGCTGC TGCTGCTGCT GTTGCTGGGA GCCATAGTGA CTCTGGGTGT CTTCTACAGC 
ATAGGGAAGC AGCTGCCCCT CTTAACTAGC CTGCTACACT TCTCCTGGGA TGAGAGGACG 
GTTGTAAAAC GAGCCCTCAG GGATTCATCA CTGAAAAGTG ACATCTGCAC CACCCCAAGC 
TGTGTGATAG CAGCTGCCAG AATCCTCGAA AACATGGACC AATCGAGGAA CCCCTGTGAA 
AACTTCTACC AGTACGCTTG CGGAGGCTGG CTGAGGCACC ACGTGATCCC AGAGACCAAC 
TCCAGATACA GCGTCTTTGA CATCCTTCGG GACGAGCTGG AGGTTATCCT CAAAGGGGTG 
CTGGAGGATT CCACTTCCCA GCATCGCCCG GCCGTGGAGA AGGCCAAGAC ACTATATCGC 
TCCTGCATGA ACCAAAGTGT GATCGAGAAG AGAGACTCTG AGCCCCTGCT GAGCGTCTTA 
AAAATGGTAG GAGGTTGGCC TGTGGCCATG GATAAGTGGA ACGAGACCAT GGGCCTCAAG 
TGGGAACTGG AGCGACAGTT GGCTGTGTTG AACTCGCAGT TCAACAGGCG GGTCCTCATC 
GACCTCTTCA TCTGGAATGA CGACCAGAAC TCCAGCCGGC ATGTCATCTA CATAGACCAG 
CCCACCTTGG GCATGCCATC CCGGGAGTAC TATTTCCAGG AGGACAACAA CCACAAGGTA 
CGGAAAGCCT ACCTGGAGTT CATGACGTCA GTGGCCACTA TGCTTAGGAA AGACCAGAAC 
CTGTCCAAGG AGAGCGCCAT GGTGCGGGAG GAGATGGCGG AGGTGCTGGA ACTGGAGACG 
CATCTGGCCA ACGCCACAGT CCCCCAGGAG AAAAGGCATG ATGTCACTGC CCTGTACCAC 
CGAATGGACC TGATGGAGCT ACAGGAAAGG TTTGGTCTGA AGGGGTTTAA CTGGACTCTC 
TTCATACAAA ACGTGTTGTC TTCTGTGGAA GTCGAGCTGT TCCCAGATGA GGAGGTGGTG 
GTCTACGGCA TCCCCTACCT GGAGAATCTG GAGGATATCA TTGATAGCTA CTCAGCACGG 
ACCATGCAGA ACTACCTGGT ATGGCGCCTG GTGCTAGATC GAATTGGCAG CCTGAGCCAG 
AGATTCAAAG AGGCGCGTGT GGACTACCGC AAGGCGCTGT ACGGCACGAC CGTGGAGGAG 
GTACGCTGGC GAGAGTGTGT CAGCTATGTC AACAGTAACA TGGAGAGCGC CGTGGGCTCC 
CTCTACATCA AGCGGGCCTT CTCCAAGGAC AGCAAGAGCA CGGTCAGAGA GCTGATTGAG 
AAGATAAGGT CCGTGTTTGT GGATAACCTG GATGAGCTGA ACTGGATGGA CGAGGAATCC 
AAGAAGAAGG CCCAGGAAAA GGCCATGAAT ATACGGGAAC AGATTGGCTA CCCTGACTAC 
ATTTTGGAAG ATAACAATAA ACACCTGGAT GAGGAATACT CCAGTTTGAC TTTCTATGAG 
GACCTGTATT TTGAGAACGG ACTTCAGAAC CTCAAGAACA ATGCCCAGAG GAGCCTCAAG 
AAGCTTCGGG AAAAGGTGGA CCAGAATCTC TGGATCATCG GGGCTGCAGT GGTCAATGCA 
TTCTACTCCC CAAACAGAAA CCAGATCGTC TTTCCAGCAG GGATTCTCCA GCCGCCCTTC 
TTCAGCAAGG ACCAACCACA GTCCTTGAAT TTTGGGGGCA TCGGGATGGT GATTGGGCAC 
GAGATCACAC ACGGCTTTGA TGATAATGGT CGTAACTTTG ACAAGAACGG CAACATGCTG 
GACTGGTGGA GTAACTTCTC GGCCCGGCAC TTCCAACAGC AGTCGCAATG CATGATCTAT 
CAGTACGGCA ACTTCTCTTG GGAACTAGCA GACAACCAGA ATGTGAACGG ATTCAGTACC 
CTCGGGGAGA ACATTGCCGA CAACGGAGGT GTGCGACAGG CATACAAGGC TTACCTACGG 
TGGCTGGCTG ATGGCGGCAA AGATCAGCGA CTGCCGGGAC TGAACCTGAC CTATGCCCAG 
CTTTTCTTCA TCAACTATGC CCAGGTGTGG TGTGGGTCCT ATAGGCCGGA GTTCGCCGTC 
CAGTCCATCA AGACGGACGT CCACAGTCCT CTTAAGTACA GGGTGCTGGG CTCACTACAG 
AACCTGCCAG GCTTCTCTGA GGCATTCCAC TGCCCACGAG GCAGCCCCAT GCACCCCATG 
AAGCGATGTC GCATCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038811.2
CDS	332..2632
Translation	MVERAGWCRKKSPGFVEYGLMVLLLLLLLGAIVTLGVFYSIGKQLPLLTSLLHFSWDERTVVKRALRDSSLKSDICTTPSCVIAAARILENMDQSRNPCENFYQYACGGWLRHHVIPETNSRYSVFDILRDELEVILKGVLEDSTSQHRPAVEKAKTLYRSCMNQSVIEKRDSEPLLSVLKMVGGWPVAMDKWNETMGLKWELERQLAVLNSQFNRRVLIDLFIWNDDQNSSRHVIYIDQPTLGMPSREYYFQEDNNHKVRKAYLEFMTSVATMLRKDQNLSKESAMVREEMAEVLELETHLANATVPQEKRHDVTALYHRMDLMELQERFGLKGFNWTLFIQNVLSSVEVELFPDEEVVVYGIPYLENLEDIIDSYSARTMQNYLVWRLVLDRIGSLSQRFKEARVDYRKALYGTTVEEVRWRECVSYVNSNMESAVGSLYIKRAFSKDSKSTVRELIEKIRSVFVDNLDELNWMDEESKKKAQEKAMNIREQIGYPDYILEDNNKHLDEEYSSLTFYEDLYFENGLQNLKNNAQRSLKKLREKVDQNLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKDQPQSLNFGGIGMVIGHEITHGFDDNGRNFDKNGNMLDWWSNFSARHFQQQSQCMIYQYGNFSWELADNQNVNGFSTLGENIADNGGVRQAYKAYLRWLADGGKDQRLPGLNLTYAQLFFINYAQVWCGSYRPEFAVQSIKTDVHSPLKYRVLGSLQNLPGFSEAFHCPRGSPMHPMKRCRIW

Target ORF information:

RefSeq Version	NM_013783.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus membrane metallo-endopeptidase-like 1 (Mmel1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013783.2

ORF Insert Sequence:

ATGGTGGAGA GAGCAGGCTG GTGTCGGAAG AAGTCCCCAG GCTTCGTGGA GTATGGGCTG 
ATGGTGCTGC TGCTGCTGCT GTTGCTGGGA GCCATAGTGA CTCTGGGTGT CTTCTACAGC 
ATAGGGAAGC AGCTGCCCCT CTTAACTAGC CTGCTACACT TCTCCTGGGA TGAGAGGACG 
GTTGTAAAAC GAGCCCTCAG GGATTCATCA CTGAAAAGTG ACATCTGCAC CACCCCAAGC 
TGTGTGATAG CAGCTGCCAG AATCCTCGAA AACATGGACC AATCGAGGAA CCCCTGTGAA 
AACTTCTACC AGTACGCTTG CGGAGGCTGG CTGAGGCACC ACGTGATCCC AGAGACCAAC 
TCCAGATACA GCGTCTTTGA CATCCTTCGG GACGAGCTGG AGGTTATCCT CAAAGGGGTG 
CTGGAGGATT CCACTTCCCA GCATCGCCCG GCCGTGGAGA AGGCCAAGAC ACTATATCGC 
TCCTGCATGA ACCAAAGTGT GATCGAGAAG AGAGACTCTG AGCCCCTGCT GAGCGTCTTA 
AAAATGGTAG GAGGTTGGCC TGTGGCCATG GATAAGTGGA ACGAGACCAT GGGCCTCAAG 
TGGGAACTGG AGCGACAGTT GGCTGTGTTG AACTCGCAGT TCAACAGGCG GGTCCTCATC 
GACCTCTTCA TCTGGAATGA CGACCAGAAC TCCAGCCGGC ATGTCATCTA CATAGACCAG 
CCCACCTTGG GCATGCCATC CCGGGAGTAC TATTTCCAGG AGGACAACAA CCACAAGGTA 
CGGAAAGCCT ACCTGGAGTT CATGACGTCA GTGGCCACTA TGCTTAGGAA AGACCAGAAC 
CTGTCCAAGG AGAGCGCCAT GGTGCGGGAG GAGATGGCGG AGGTGCTGGA ACTGGAGACG 
CATCTGGCCA ACGCCACAGT CCCCCAGGAG AAAAGGCATG ATGTCACTGC CCTGTACCAC 
CGAATGGACC TGATGGAGCT ACAGGAAAGG TTTGGTCTGA AGGGGTTTAA CTGGACTCTC 
TTCATACAAA ACGTGTTGTC TTCTGTGGAA GTCGAGCTGT TCCCAGATGA GGAGGTGGTG 
GTCTACGGCA TCCCCTACCT GGAGAATCTG GAGGATATCA TTGATAGCTA CTCAGCACGG 
ACCATGCAGA ACTACCTGGT ATGGCGCCTG GTGCTAGATC GAATTGGCAG CCTGAGCCAG 
AGATTCAAAG AGGCGCGTGT GGACTACCGC AAGGCGCTGT ACGGCACGAC CGTGGAGGAG 
GTACGCTGGC GAGAGTGTGT CAGCTATGTC AACAGTAACA TGGAGAGCGC CGTGGGCTCC 
CTCTACATCA AGCGGGCCTT CTCCAAGGAC AGCAAGAGCA CGGTCAGAGA GCTGATTGAG 
AAGATAAGGT CCGTGTTTGT GGATAACCTG GATGAGCTGA ACTGGATGGA CGAGGAATCC 
AAGAAGAAGG CCCAGGAAAA GGCCATGAAT ATACGGGAAC AGATTGGCTA CCCTGACTAC 
ATTTTGGAAG ATAACAATAA ACACCTGGAT GAGGAATACT CCAGTTTGAC TTTCTATGAG 
GACCTGTATT TTGAGAACGG ACTTCAGAAC CTCAAGAACA ATGCCCAGAG GAGCCTCAAG 
AAGCTTCGGG AAAAGGTGGA CCAGAATCTC TGGATCATCG GGGCTGCAGT GGTCAATGCA 
TTCTACTCCC CAAACAGAAA CCAGATCGTC TTTCCAGCAG GGATTCTCCA GCCGCCCTTC 
TTCAGCAAGG ACCAACCACA GTCCTTGAAT TTTGGGGGCA TCGGGATGGT GATTGGGCAC 
GAGATCACAC ACGGCTTTGA TGATAATGGT CGTAACTTTG ACAAGAACGG CAACATGCTG 
GACTGGTGGA GTAACTTCTC GGCCCGGCAC TTCCAACAGC AGTCGCAATG CATGATCTAT 
CAGTACGGCA ACTTCTCTTG GGAACTAGCA GACAACCAGA ATGTGAACGG ATTCAGTACC 
CTCGGGGAGA ACATTGCCGA CAACGGAGGT GTGCGACAGG CATACAAGGC TTACCTACGG 
TGGCTGGCTG ATGGCGGCAA AGATCAGCGA CTGCCGGGAC TGAACCTGAC CTATGCCCAG 
CTTTTCTTCA TCAACTATGC CCAGGTGTGG TGTGGGTCCT ATAGGCCGGA GTTCGCCGTC 
CAGTCCATCA AGACGGACGT CCACAGTCCT CTTAAGTACA GGGTGCTGGG CTCACTACAG 
AACCTGCCAG GCTTCTCTGA GGCATTCCAC TGCCCACGAG GCAGCCCCAT GCACCCCATG 
AAGCGATGTC GCATCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sustained Activation of Rho GTPases Promotes a Synthetic Pulmonary Artery Smooth Muscle Cell Phenotype in Neprilysin Null Mice.
Arteriosclerosis, thrombosis, and vascular biology38(1)154-163(2018 Jan)
Karoor V,Fini MA,Loomis Z,Sullivan T,Hersh LB,Gerasimovskaya E,Irwin D,Dempsey EC

Protection against vascular leak in neprilysin transgenic mice with complex?overexpression?pattern.
Transgenic research25(6)773-784(2016 12)
Wick MJ,Loomis ZL,Harral JW,Le M,Wehling CA,Miller YE,Dempsey EC

Accumulation of murine amyloid-? mimics early Alzheimer's disease.
Brain : a journal of neurology138(Pt 8)2370-82(2015 Aug)
Krohn M,Bracke A,Avchalumov Y,Schumacher T,Hofrichter J,Paarmann K,Fr?hlich C,Lange C,Br?ning T,von Bohlen Und Halbach O,Pahnke J

Cortical fast-spiking parvalbumin interneurons enwrapped in the perineuronal net express the metallopeptidases Adamts8, Adamts15 and Neprilysin.
Molecular psychiatry20(2)154-61(2015 Feb)
Rossier J,Bernard A,Cabungcal JH,Perrenoud Q,Savoye A,Gallopin T,Hawrylycz M,Cu?nod M,Do K,Urban A,Lein ES

Neprilysin-2 is an important ?-amyloid degrading enzyme.
The American journal of pathology178(1)306-12(2011 Jan)
Hafez D,Huang JY,Huynh AM,Valtierra S,Rockenstein E,Bruno AM,Lu B,DesGroseillers L,Masliah E,Marr RA

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Mmel1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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