MMEL1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017002314.1	XP_016857803.1	membrane metallo-endopeptidase-like 1 isoform X5
XM_011542122.2	XP_011540424.1	membrane metallo-endopeptidase-like 1 isoform X7
NM_033467.3	NP_258428.2	membrane metallo-endopeptidase-like 1
XM_017002315.1	XP_016857804.1	membrane metallo-endopeptidase-like 1 isoform X6
XM_017002310.1	XP_016857799.1	membrane metallo-endopeptidase-like 1 isoform X1
XM_017002312.1	XP_016857801.1	membrane metallo-endopeptidase-like 1 isoform X3
XM_017002311.1	XP_016857800.1	membrane metallo-endopeptidase-like 1 isoform X2
XM_017002313.1	XP_016857802.1	membrane metallo-endopeptidase-like 1 isoform X4
NM_033467.4	NP_258428.2	membrane metallo-endopeptidase-like 1

The following MMEL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMEL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14746	NM_033467.4 Latest version!	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu14746	XM_017002310.1 Latest version!	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu71833	XM_011542122.2 Latest version!	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu90514	XM_017002315.1 Latest version!	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OHu90510	XM_017002311.1 Latest version!	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu90511	XM_017002312.1 Latest version!	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu90512	XM_017002313.1 Latest version!	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu90513	XM_017002314.1 Latest version!	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu14746	NM_033467.3	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

Reference Sequences (Refseq)

MMEL1 ( NM_033467.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_258428.2 Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), mRNA.

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CloneID	OHu14746
Clone ID Related Accession (Same CDS sequence)	NM_033467.3 , XM_017002310.1 , NM_033467.4
Accession Version	NM_033467.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	membrane metallo-endopeptidase-like 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB089501.1, BC101027.2, AF336981.1 and AK093058.1. On Jun 6, 2009 this sequence version replaced NM_033467.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK093058.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGT CCGAAGGCCC AGTGGGGATG GTGGAGAGCG CCGGCCGTGC AGGGCAGAAG 
CGCCCGGGGT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TGCTGGTGAC CGCTGCCCTG 
GTGGCCTTGG GTGTCCTCTA CGCCGACCGC AGAGGGAAGC AGCTGCCACG CCTTGCTAGC 
CGGCTGTGCT TCTTACAGGA GGAGAGGACC TTTGTAAAAC GAAAACCCCG AGGGATCCCA 
GAGGCCCAAG AGGTGAGCGA GGTCTGCACC ACCCCTGGCT GCGTGATAGC AGCTGCCAGG 
ATCCTCCAGA ACATGGACCC GACCACGGAA CCGTGTGACG ACTTCTACCA GTTTGCATGC 
GGAGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CAAGATACAG CATCTTTGAC 
GTCCTCCGCG ACGAGCTGGA GGTCATCCTC AAAGCGGTGC TGGAGAATTC GACTGCCAAG 
GACCGGCCGG CTGTGGAGAA GGCCAGGACG CTGTACCGCT CCTGCATGAA CCAGAGTGTG 
ATAGAGAAGC GAGGCTCTCA GCCCCTGCTG GACATCTTGG AGGTGGTGGG AGGCTGGCCG 
GTGGCGATGG ACAGGTGGAA CGAGACCGTA GGACTCGAGT GGGAGCTGGA GCGGCAGCTG 
GCGCTGATGA ACTCACAGTT CAACAGGCGC GTCCTCATCG ACCTCTTCAT CTGGAACGAC 
GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAGC CCACCTTGGG CATGCCCTCC 
CGAGAGTACT ACTTCAACGG CGGCAGCAAC CGGAAGGTGC GGGAAGCCTA CCTGCAGTTC 
ATGGTGTCAG TGGCCACGTT GCTGCGGGAG GATGCAAACC TGCCCAGGGA CAGCTGCCTG 
GTGCAGGAGG ACATGGTGCA GGTGCTGGAG CTGGAGACAC AGCTGGCCAA GGCCACGGTA 
CCCCAGGAGG AGAGACACGA CGTCATCGCC TTGTACCACC GGATGGGACT GGAGGAGCTG 
CAAAGCCAGT TTGGCCTGAA GGGATTTAAC TGGACTCTGT TCATACAAAC TGTGCTATCC 
TCTGTCAAAA TCAAGCTGCT GCCAGATGAG GAAGTGGTGG TCTATGGCAT CCCCTACCTG 
CAGAACCTTG AAAACATCAT CGACACCTAC TCAGCCAGGA CCATACAGAA CTACCTGGTC 
TGGCGCCTGG TGCTGGACCG CATTGGTAGC CTAAGCCAGA GATTCAAGGA CACACGAGTG 
AACTACCGCA AGGCGCTGTT TGGCACAATG GTGGAGGAGG TGCGCTGGCG TGAATGTGTG 
GGCTACGTCA ACAGCAACAT GGAGAACGCC GTGGGCTCCC TCTACGTCAG GGAGGCGTTC 
CCTGGAGACA GCAAGAGCAT GGTCAGAGAA CTCATTGACA AGGTGCGGAC AGTGTTTGTG 
GAGACGCTGG ACGAGCTGGG CTGGATGGAC GAGGAGTCCA AGAAGAAGGC GCAGGAGAAG 
GCCATGAGCA TCCGGGAGCA GATCGGGCAC CCTGACTACA TCCTGGAGGA GATGAACAGG 
CGCCTGGACG AGGAGTACTC CAATCTGAAC TTCTCAGAGG ACCTGTACTT TGAGAACAGT 
CTGCAGAACC TCAAGGTGGG CGCCCAGCGG AGCCTCAGGA AGCTTCGGGA AAAGGTGGAC 
CCAAATCTCT GGATCATCGG GGCGGCGGTG GTCAATGCGT TCTACTCCCC AAACCGAAAC 
CAGATTGTAT TCCCTGCCGG GATCCTCCAG CCCCCCTTCT TCAGCAAGGA GCAGCCACAG 
GCCTTGAACT TTGGAGGCAT TGGGATGGTG ATCGGGCACG AGATCACGCA CGGCTTTGAC 
GACAATGGCC GGAACTTCGA CAAGAATGGC AACATGATGG ATTGGTGGAG TAACTTCTCC 
ACCCAGCACT TCCGGGAGCA GTCAGAGTGC ATGATCTACC AGTACGGCAA CTACTCCTGG 
GACCTGGCAG ACGAACAGAA CGTGAACGGA TTCAACACCC TTGGGGAAAA CATTGCTGAC 
AACGGAGGGG TGCGGCAAGC CTATAAGGCC TACCTCAAGT GGATGGCAGA GGGTGGCAAG 
GACCAGCAGC TGCCCGGCCT GGATCTCACC CATGAGCAGC TCTTCTTCAT CAACTATGCC 
CAGGTGTGGT GCGGGTCCTA CCGGCCCGAG TTCGCCATCC AATCCATCAA GACAGACGTC 
CACAGTCCCC TGAAGTACAG GGTACTGGGG TCGCTGCAGA ACCTGGCCGC CTTCGCAGAC 
ACGTTCCACT GTGCCCGGGG CACCCCCATG CACCCCAAGG AGCGATGCCG CGTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_258428.2
CDS	215..2554
Translation	MGKSEGPVGMVESAGRAGQKRPGFLEGGLLLLLLLVTAALVALGVLYADRRGKQLPRLASRLCFLQEERTFVKRKPRGIPEAQEVSEVCTTPGCVIAAARILQNMDPTTEPCDDFYQFACGGWLRRHVIPETNSRYSIFDVLRDELEVILKAVLENSTAKDRPAVEKARTLYRSCMNQSVIEKRGSQPLLDILEVVGGWPVAMDRWNETVGLEWELERQLALMNSQFNRRVLIDLFIWNDDQNSSRHIIYIDQPTLGMPSREYYFNGGSNRKVREAYLQFMVSVATLLREDANLPRDSCLVQEDMVQVLELETQLAKATVPQEERHDVIALYHRMGLEELQSQFGLKGFNWTLFIQTVLSSVKIKLLPDEEVVVYGIPYLQNLENIIDTYSARTIQNYLVWRLVLDRIGSLSQRFKDTRVNYRKALFGTMVEEVRWRECVGYVNSNMENAVGSLYVREAFPGDSKSMVRELIDKVRTVFVETLDELGWMDEESKKKAQEKAMSIREQIGHPDYILEEMNRRLDEEYSNLNFSEDLYFENSLQNLKVGAQRSLRKLREKVDPNLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKEQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMMDWWSNFSTQHFREQSECMIYQYGNYSWDLADEQNVNGFNTLGENIADNGGVRQAYKAYLKWMAEGGKDQQLPGLDLTHEQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLAAFADTFHCARGTPMHPKERCRVW

Target ORF information:

RefSeq Version	NM_033467.3
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033467.3

ORF Insert Sequence:

ATGGGGAAGT CCGAAGGCCC AGTGGGGATG GTGGAGAGCG CCGGCCGTGC AGGGCAGAAG 
CGCCCGGGGT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TGCTGGTGAC CGCTGCCCTG 
GTGGCCTTGG GTGTCCTCTA CGCCGACCGC AGAGGGAAGC AGCTGCCACG CCTTGCTAGC 
CGGCTGTGCT TCTTACAGGA GGAGAGGACC TTTGTAAAAC GAAAACCCCG AGGGATCCCA 
GAGGCCCAAG AGGTGAGCGA GGTCTGCACC ACCCCTGGCT GCGTGATAGC AGCTGCCAGG 
ATCCTCCAGA ACATGGACCC GACCACGGAA CCGTGTGACG ACTTCTACCA GTTTGCATGC 
GGAGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CAAGATACAG CATCTTTGAC 
GTCCTCCGCG ACGAGCTGGA GGTCATCCTC AAAGCGGTGC TGGAGAATTC GACTGCCAAG 
GACCGGCCGG CTGTGGAGAA GGCCAGGACG CTGTACCGCT CCTGCATGAA CCAGAGTGTG 
ATAGAGAAGC GAGGCTCTCA GCCCCTGCTG GACATCTTGG AGGTGGTGGG AGGCTGGCCG 
GTGGCGATGG ACAGGTGGAA CGAGACCGTA GGACTCGAGT GGGAGCTGGA GCGGCAGCTG 
GCGCTGATGA ACTCACAGTT CAACAGGCGC GTCCTCATCG ACCTCTTCAT CTGGAACGAC 
GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAGC CCACCTTGGG CATGCCCTCC 
CGAGAGTACT ACTTCAACGG CGGCAGCAAC CGGAAGGTGC GGGAAGCCTA CCTGCAGTTC 
ATGGTGTCAG TGGCCACGTT GCTGCGGGAG GATGCAAACC TGCCCAGGGA CAGCTGCCTG 
GTGCAGGAGG ACATGGTGCA GGTGCTGGAG CTGGAGACAC AGCTGGCCAA GGCCACGGTA 
CCCCAGGAGG AGAGACACGA CGTCATCGCC TTGTACCACC GGATGGGACT GGAGGAGCTG 
CAAAGCCAGT TTGGCCTGAA GGGATTTAAC TGGACTCTGT TCATACAAAC TGTGCTATCC 
TCTGTCAAAA TCAAGCTGCT GCCAGATGAG GAAGTGGTGG TCTATGGCAT CCCCTACCTG 
CAGAACCTTG AAAACATCAT CGACACCTAC TCAGCCAGGA CCATACAGAA CTACCTGGTC 
TGGCGCCTGG TGCTGGACCG CATTGGTAGC CTAAGCCAGA GATTCAAGGA CACACGAGTG 
AACTACCGCA AGGCGCTGTT TGGCACAATG GTGGAGGAGG TGCGCTGGCG TGAATGTGTG 
GGCTACGTCA ACAGCAACAT GGAGAACGCC GTGGGCTCCC TCTACGTCAG GGAGGCGTTC 
CCTGGAGACA GCAAGAGCAT GGTCAGAGAA CTCATTGACA AGGTGCGGAC AGTGTTTGTG 
GAGACGCTGG ACGAGCTGGG CTGGATGGAC GAGGAGTCCA AGAAGAAGGC GCAGGAGAAG 
GCCATGAGCA TCCGGGAGCA GATCGGGCAC CCTGACTACA TCCTGGAGGA GATGAACAGG 
CGCCTGGACG AGGAGTACTC CAATCTGAAC TTCTCAGAGG ACCTGTACTT TGAGAACAGT 
CTGCAGAACC TCAAGGTGGG CGCCCAGCGG AGCCTCAGGA AGCTTCGGGA AAAGGTGGAC 
CCAAATCTCT GGATCATCGG GGCGGCGGTG GTCAATGCGT TCTACTCCCC AAACCGAAAC 
CAGATTGTAT TCCCTGCCGG GATCCTCCAG CCCCCCTTCT TCAGCAAGGA GCAGCCACAG 
GCCTTGAACT TTGGAGGCAT TGGGATGGTG ATCGGGCACG AGATCACGCA CGGCTTTGAC 
GACAATGGCC GGAACTTCGA CAAGAATGGC AACATGATGG ATTGGTGGAG TAACTTCTCC 
ACCCAGCACT TCCGGGAGCA GTCAGAGTGC ATGATCTACC AGTACGGCAA CTACTCCTGG 
GACCTGGCAG ACGAACAGAA CGTGAACGGA TTCAACACCC TTGGGGAAAA CATTGCTGAC 
AACGGAGGGG TGCGGCAAGC CTATAAGGCC TACCTCAAGT GGATGGCAGA GGGTGGCAAG 
GACCAGCAGC TGCCCGGCCT GGATCTCACC CATGAGCAGC TCTTCTTCAT CAACTATGCC 
CAGGTGTGGT GCGGGTCCTA CCGGCCCGAG TTCGCCATCC AATCCATCAA GACAGACGTC 
CACAGTCCCC TGAAGTACAG GGTACTGGGG TCGCTGCAGA ACCTGGCCGC CTTCGCAGAC 
ACGTTCCACT GTGCCCGGGG CACCCCCATG CACCCCAAGG AGCGATGCCG CGTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMEL1 ( NM_033467.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_258428.2 Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), mRNA.

Price & Availability↑

CloneID	OHu14746
Clone ID Related Accession (Same CDS sequence)	NM_033467.3 , XM_017002310.1 , NM_033467.4
Accession Version	NM_033467.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	membrane metallo-endopeptidase-like 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB089501.1, BC101027.2, AF336981.1, AK093058.1 and AL139246.21. On Nov 23, 2018 this sequence version replaced NM_033467.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK093058.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000378412.8/ ENSP00000367668.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGAAGT CCGAAGGCCC AGTGGGGATG GTGGAGAGCG CCGGCCGTGC AGGGCAGAAG 
CGCCCGGGGT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TGCTGGTGAC CGCTGCCCTG 
GTGGCCTTGG GTGTCCTCTA CGCCGACCGC AGAGGGAAGC AGCTGCCACG CCTTGCTAGC 
CGGCTGTGCT TCTTACAGGA GGAGAGGACC TTTGTAAAAC GAAAACCCCG AGGGATCCCA 
GAGGCCCAAG AGGTGAGCGA GGTCTGCACC ACCCCTGGCT GCGTGATAGC AGCTGCCAGG 
ATCCTCCAGA ACATGGACCC GACCACGGAA CCGTGTGACG ACTTCTACCA GTTTGCATGC 
GGAGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CAAGATACAG CATCTTTGAC 
GTCCTCCGCG ACGAGCTGGA GGTCATCCTC AAAGCGGTGC TGGAGAATTC GACTGCCAAG 
GACCGGCCGG CTGTGGAGAA GGCCAGGACG CTGTACCGCT CCTGCATGAA CCAGAGTGTG 
ATAGAGAAGC GAGGCTCTCA GCCCCTGCTG GACATCTTGG AGGTGGTGGG AGGCTGGCCG 
GTGGCGATGG ACAGGTGGAA CGAGACCGTA GGACTCGAGT GGGAGCTGGA GCGGCAGCTG 
GCGCTGATGA ACTCACAGTT CAACAGGCGC GTCCTCATCG ACCTCTTCAT CTGGAACGAC 
GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAGC CCACCTTGGG CATGCCCTCC 
CGAGAGTACT ACTTCAACGG CGGCAGCAAC CGGAAGGTGC GGGAAGCCTA CCTGCAGTTC 
ATGGTGTCAG TGGCCACGTT GCTGCGGGAG GATGCAAACC TGCCCAGGGA CAGCTGCCTG 
GTGCAGGAGG ACATGGTGCA GGTGCTGGAG CTGGAGACAC AGCTGGCCAA GGCCACGGTA 
CCCCAGGAGG AGAGACACGA CGTCATCGCC TTGTACCACC GGATGGGACT GGAGGAGCTG 
CAAAGCCAGT TTGGCCTGAA GGGATTTAAC TGGACTCTGT TCATACAAAC TGTGCTATCC 
TCTGTCAAAA TCAAGCTGCT GCCAGATGAG GAAGTGGTGG TCTATGGCAT CCCCTACCTG 
CAGAACCTTG AAAACATCAT CGACACCTAC TCAGCCAGGA CCATACAGAA CTACCTGGTC 
TGGCGCCTGG TGCTGGACCG CATTGGTAGC CTAAGCCAGA GATTCAAGGA CACACGAGTG 
AACTACCGCA AGGCGCTGTT TGGCACAATG GTGGAGGAGG TGCGCTGGCG TGAATGTGTG 
GGCTACGTCA ACAGCAACAT GGAGAACGCC GTGGGCTCCC TCTACGTCAG GGAGGCGTTC 
CCTGGAGACA GCAAGAGCAT GGTCAGAGAA CTCATTGACA AGGTGCGGAC AGTGTTTGTG 
GAGACGCTGG ACGAGCTGGG CTGGATGGAC GAGGAGTCCA AGAAGAAGGC GCAGGAGAAG 
GCCATGAGCA TCCGGGAGCA GATCGGGCAC CCTGACTACA TCCTGGAGGA GATGAACAGG 
CGCCTGGACG AGGAGTACTC CAATCTGAAC TTCTCAGAGG ACCTGTACTT TGAGAACAGT 
CTGCAGAACC TCAAGGTGGG CGCCCAGCGG AGCCTCAGGA AGCTTCGGGA AAAGGTGGAC 
CCAAATCTCT GGATCATCGG GGCGGCGGTG GTCAATGCGT TCTACTCCCC AAACCGAAAC 
CAGATTGTAT TCCCTGCCGG GATCCTCCAG CCCCCCTTCT TCAGCAAGGA GCAGCCACAG 
GCCTTGAACT TTGGAGGCAT TGGGATGGTG ATCGGGCACG AGATCACGCA CGGCTTTGAC 
GACAATGGCC GGAACTTCGA CAAGAATGGC AACATGATGG ATTGGTGGAG TAACTTCTCC 
ACCCAGCACT TCCGGGAGCA GTCAGAGTGC ATGATCTACC AGTACGGCAA CTACTCCTGG 
GACCTGGCAG ACGAACAGAA CGTGAACGGA TTCAACACCC TTGGGGAAAA CATTGCTGAC 
AACGGAGGGG TGCGGCAAGC CTATAAGGCC TACCTCAAGT GGATGGCAGA GGGTGGCAAG 
GACCAGCAGC TGCCCGGCCT GGATCTCACC CATGAGCAGC TCTTCTTCAT CAACTATGCC 
CAGGTGTGGT GCGGGTCCTA CCGGCCCGAG TTCGCCATCC AATCCATCAA GACAGACGTC 
CACAGTCCCC TGAAGTACAG GGTACTGGGG TCGCTGCAGA ACCTGGCCGC CTTCGCAGAC 
ACGTTCCACT GTGCCCGGGG CACCCCCATG CACCCCAAGG AGCGATGCCG CGTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_258428.2
CDS	189..2528
Translation	MGKSEGPVGMVESAGRAGQKRPGFLEGGLLLLLLLVTAALVALGVLYADRRGKQLPRLASRLCFLQEERTFVKRKPRGIPEAQEVSEVCTTPGCVIAAARILQNMDPTTEPCDDFYQFACGGWLRRHVIPETNSRYSIFDVLRDELEVILKAVLENSTAKDRPAVEKARTLYRSCMNQSVIEKRGSQPLLDILEVVGGWPVAMDRWNETVGLEWELERQLALMNSQFNRRVLIDLFIWNDDQNSSRHIIYIDQPTLGMPSREYYFNGGSNRKVREAYLQFMVSVATLLREDANLPRDSCLVQEDMVQVLELETQLAKATVPQEERHDVIALYHRMGLEELQSQFGLKGFNWTLFIQTVLSSVKIKLLPDEEVVVYGIPYLQNLENIIDTYSARTIQNYLVWRLVLDRIGSLSQRFKDTRVNYRKALFGTMVEEVRWRECVGYVNSNMENAVGSLYVREAFPGDSKSMVRELIDKVRTVFVETLDELGWMDEESKKKAQEKAMSIREQIGHPDYILEEMNRRLDEEYSNLNFSEDLYFENSLQNLKVGAQRSLRKLREKVDPNLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKEQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMMDWWSNFSTQHFREQSECMIYQYGNYSWDLADEQNVNGFNTLGENIADNGGVRQAYKAYLKWMAEGGKDQQLPGLDLTHEQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLAAFADTFHCARGTPMHPKERCRVW

Target ORF information:

RefSeq Version	NM_033467.4
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033467.4

ORF Insert Sequence:

ATGGGGAAGT CCGAAGGCCC AGTGGGGATG GTGGAGAGCG CCGGCCGTGC AGGGCAGAAG 
CGCCCGGGGT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TGCTGGTGAC CGCTGCCCTG 
GTGGCCTTGG GTGTCCTCTA CGCCGACCGC AGAGGGAAGC AGCTGCCACG CCTTGCTAGC 
CGGCTGTGCT TCTTACAGGA GGAGAGGACC TTTGTAAAAC GAAAACCCCG AGGGATCCCA 
GAGGCCCAAG AGGTGAGCGA GGTCTGCACC ACCCCTGGCT GCGTGATAGC AGCTGCCAGG 
ATCCTCCAGA ACATGGACCC GACCACGGAA CCGTGTGACG ACTTCTACCA GTTTGCATGC 
GGAGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CAAGATACAG CATCTTTGAC 
GTCCTCCGCG ACGAGCTGGA GGTCATCCTC AAAGCGGTGC TGGAGAATTC GACTGCCAAG 
GACCGGCCGG CTGTGGAGAA GGCCAGGACG CTGTACCGCT CCTGCATGAA CCAGAGTGTG 
ATAGAGAAGC GAGGCTCTCA GCCCCTGCTG GACATCTTGG AGGTGGTGGG AGGCTGGCCG 
GTGGCGATGG ACAGGTGGAA CGAGACCGTA GGACTCGAGT GGGAGCTGGA GCGGCAGCTG 
GCGCTGATGA ACTCACAGTT CAACAGGCGC GTCCTCATCG ACCTCTTCAT CTGGAACGAC 
GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAGC CCACCTTGGG CATGCCCTCC 
CGAGAGTACT ACTTCAACGG CGGCAGCAAC CGGAAGGTGC GGGAAGCCTA CCTGCAGTTC 
ATGGTGTCAG TGGCCACGTT GCTGCGGGAG GATGCAAACC TGCCCAGGGA CAGCTGCCTG 
GTGCAGGAGG ACATGGTGCA GGTGCTGGAG CTGGAGACAC AGCTGGCCAA GGCCACGGTA 
CCCCAGGAGG AGAGACACGA CGTCATCGCC TTGTACCACC GGATGGGACT GGAGGAGCTG 
CAAAGCCAGT TTGGCCTGAA GGGATTTAAC TGGACTCTGT TCATACAAAC TGTGCTATCC 
TCTGTCAAAA TCAAGCTGCT GCCAGATGAG GAAGTGGTGG TCTATGGCAT CCCCTACCTG 
CAGAACCTTG AAAACATCAT CGACACCTAC TCAGCCAGGA CCATACAGAA CTACCTGGTC 
TGGCGCCTGG TGCTGGACCG CATTGGTAGC CTAAGCCAGA GATTCAAGGA CACACGAGTG 
AACTACCGCA AGGCGCTGTT TGGCACAATG GTGGAGGAGG TGCGCTGGCG TGAATGTGTG 
GGCTACGTCA ACAGCAACAT GGAGAACGCC GTGGGCTCCC TCTACGTCAG GGAGGCGTTC 
CCTGGAGACA GCAAGAGCAT GGTCAGAGAA CTCATTGACA AGGTGCGGAC AGTGTTTGTG 
GAGACGCTGG ACGAGCTGGG CTGGATGGAC GAGGAGTCCA AGAAGAAGGC GCAGGAGAAG 
GCCATGAGCA TCCGGGAGCA GATCGGGCAC CCTGACTACA TCCTGGAGGA GATGAACAGG 
CGCCTGGACG AGGAGTACTC CAATCTGAAC TTCTCAGAGG ACCTGTACTT TGAGAACAGT 
CTGCAGAACC TCAAGGTGGG CGCCCAGCGG AGCCTCAGGA AGCTTCGGGA AAAGGTGGAC 
CCAAATCTCT GGATCATCGG GGCGGCGGTG GTCAATGCGT TCTACTCCCC AAACCGAAAC 
CAGATTGTAT TCCCTGCCGG GATCCTCCAG CCCCCCTTCT TCAGCAAGGA GCAGCCACAG 
GCCTTGAACT TTGGAGGCAT TGGGATGGTG ATCGGGCACG AGATCACGCA CGGCTTTGAC 
GACAATGGCC GGAACTTCGA CAAGAATGGC AACATGATGG ATTGGTGGAG TAACTTCTCC 
ACCCAGCACT TCCGGGAGCA GTCAGAGTGC ATGATCTACC AGTACGGCAA CTACTCCTGG 
GACCTGGCAG ACGAACAGAA CGTGAACGGA TTCAACACCC TTGGGGAAAA CATTGCTGAC 
AACGGAGGGG TGCGGCAAGC CTATAAGGCC TACCTCAAGT GGATGGCAGA GGGTGGCAAG 
GACCAGCAGC TGCCCGGCCT GGATCTCACC CATGAGCAGC TCTTCTTCAT CAACTATGCC 
CAGGTGTGGT GCGGGTCCTA CCGGCCCGAG TTCGCCATCC AATCCATCAA GACAGACGTC 
CACAGTCCCC TGAAGTACAG GGTACTGGGG TCGCTGCAGA ACCTGGCCGC CTTCGCAGAC 
ACGTTCCACT GTGCCCGGGG CACCCCCATG CACCCCAAGG AGCGATGCCG CGTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMEL1 ( XM_017002310.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857799.1 Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X1, mRNA.

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CloneID	OHu14746
Clone ID Related Accession (Same CDS sequence)	NM_033467.3 , XM_017002310.1 , NM_033467.4
Accession Version	XM_017002310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	membrane metallo-endopeptidase-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGT CCGAAGGCCC AGTGGGGATG GTGGAGAGCG CCGGCCGTGC AGGGCAGAAG 
CGCCCGGGGT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TGCTGGTGAC CGCTGCCCTG 
GTGGCCTTGG GTGTCCTCTA CGCCGACCGC AGAGGGAAGC AGCTGCCACG CCTTGCTAGC 
CGGCTGTGCT TCTTACAGGA GGAGAGGACC TTTGTAAAAC GAAAACCCCG AGGGATCCCA 
GAGGCCCAAG AGGTGAGCGA GGTCTGCACC ACCCCTGGCT GCGTGATAGC AGCTGCCAGG 
ATCCTCCAGA ACATGGACCC GACCACGGAA CCGTGTGACG ACTTCTACCA GTTTGCATGC 
GGAGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CAAGATACAG CATCTTTGAC 
GTCCTCCGCG ACGAGCTGGA GGTCATCCTC AAAGCGGTGC TGGAGAATTC GACTGCCAAG 
GACCGGCCGG CTGTGGAGAA GGCCAGGACG CTGTACCGCT CCTGCATGAA CCAGAGTGTG 
ATAGAGAAGC GAGGCTCTCA GCCCCTGCTG GACATCTTGG AGGTGGTGGG AGGCTGGCCG 
GTGGCGATGG ACAGGTGGAA CGAGACCGTA GGACTCGAGT GGGAGCTGGA GCGGCAGCTG 
GCGCTGATGA ACTCACAGTT CAACAGGCGC GTCCTCATCG ACCTCTTCAT CTGGAACGAC 
GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAGC CCACCTTGGG CATGCCCTCC 
CGAGAGTACT ACTTCAACGG CGGCAGCAAC CGGAAGGTGC GGGAAGCCTA CCTGCAGTTC 
ATGGTGTCAG TGGCCACGTT GCTGCGGGAG GATGCAAACC TGCCCAGGGA CAGCTGCCTG 
GTGCAGGAGG ACATGGTGCA GGTGCTGGAG CTGGAGACAC AGCTGGCCAA GGCCACGGTA 
CCCCAGGAGG AGAGACACGA CGTCATCGCC TTGTACCACC GGATGGGACT GGAGGAGCTG 
CAAAGCCAGT TTGGCCTGAA GGGATTTAAC TGGACTCTGT TCATACAAAC TGTGCTATCC 
TCTGTCAAAA TCAAGCTGCT GCCAGATGAG GAAGTGGTGG TCTATGGCAT CCCCTACCTG 
CAGAACCTTG AAAACATCAT CGACACCTAC TCAGCCAGGA CCATACAGAA CTACCTGGTC 
TGGCGCCTGG TGCTGGACCG CATTGGTAGC CTAAGCCAGA GATTCAAGGA CACACGAGTG 
AACTACCGCA AGGCGCTGTT TGGCACAATG GTGGAGGAGG TGCGCTGGCG TGAATGTGTG 
GGCTACGTCA ACAGCAACAT GGAGAACGCC GTGGGCTCCC TCTACGTCAG GGAGGCGTTC 
CCTGGAGACA GCAAGAGCAT GGTCAGAGAA CTCATTGACA AGGTGCGGAC AGTGTTTGTG 
GAGACGCTGG ACGAGCTGGG CTGGATGGAC GAGGAGTCCA AGAAGAAGGC GCAGGAGAAG 
GCCATGAGCA TCCGGGAGCA GATCGGGCAC CCTGACTACA TCCTGGAGGA GATGAACAGG 
CGCCTGGACG AGGAGTACTC CAATCTGAAC TTCTCAGAGG ACCTGTACTT TGAGAACAGT 
CTGCAGAACC TCAAGGTGGG CGCCCAGCGG AGCCTCAGGA AGCTTCGGGA AAAGGTGGAC 
CCAAATCTCT GGATCATCGG GGCGGCGGTG GTCAATGCGT TCTACTCCCC AAACCGAAAC 
CAGATTGTAT TCCCTGCCGG GATCCTCCAG CCCCCCTTCT TCAGCAAGGA GCAGCCACAG 
GCCTTGAACT TTGGAGGCAT TGGGATGGTG ATCGGGCACG AGATCACGCA CGGCTTTGAC 
GACAATGGCC GGAACTTCGA CAAGAATGGC AACATGATGG ATTGGTGGAG TAACTTCTCC 
ACCCAGCACT TCCGGGAGCA GTCAGAGTGC ATGATCTACC AGTACGGCAA CTACTCCTGG 
GACCTGGCAG ACGAACAGAA CGTGAACGGA TTCAACACCC TTGGGGAAAA CATTGCTGAC 
AACGGAGGGG TGCGGCAAGC CTATAAGGCC TACCTCAAGT GGATGGCAGA GGGTGGCAAG 
GACCAGCAGC TGCCCGGCCT GGATCTCACC CATGAGCAGC TCTTCTTCAT CAACTATGCC 
CAGGTGTGGT GCGGGTCCTA CCGGCCCGAG TTCGCCATCC AATCCATCAA GACAGACGTC 
CACAGTCCCC TGAAGTACAG GGTACTGGGG TCGCTGCAGA ACCTGGCCGC CTTCGCAGAC 
ACGTTCCACT GTGCCCGGGG CACCCCCATG CACCCCAAGG AGCGATGCCG CGTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857799.1
CDS	481..2820
Translation	MGKSEGPVGMVESAGRAGQKRPGFLEGGLLLLLLLVTAALVALGVLYADRRGKQLPRLASRLCFLQEERTFVKRKPRGIPEAQEVSEVCTTPGCVIAAARILQNMDPTTEPCDDFYQFACGGWLRRHVIPETNSRYSIFDVLRDELEVILKAVLENSTAKDRPAVEKARTLYRSCMNQSVIEKRGSQPLLDILEVVGGWPVAMDRWNETVGLEWELERQLALMNSQFNRRVLIDLFIWNDDQNSSRHIIYIDQPTLGMPSREYYFNGGSNRKVREAYLQFMVSVATLLREDANLPRDSCLVQEDMVQVLELETQLAKATVPQEERHDVIALYHRMGLEELQSQFGLKGFNWTLFIQTVLSSVKIKLLPDEEVVVYGIPYLQNLENIIDTYSARTIQNYLVWRLVLDRIGSLSQRFKDTRVNYRKALFGTMVEEVRWRECVGYVNSNMENAVGSLYVREAFPGDSKSMVRELIDKVRTVFVETLDELGWMDEESKKKAQEKAMSIREQIGHPDYILEEMNRRLDEEYSNLNFSEDLYFENSLQNLKVGAQRSLRKLREKVDPNLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKEQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMMDWWSNFSTQHFREQSECMIYQYGNYSWDLADEQNVNGFNTLGENIADNGGVRQAYKAYLKWMAEGGKDQQLPGLDLTHEQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLAAFADTFHCARGTPMHPKERCRVW

Target ORF information:

RefSeq Version	XM_017002310.1
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002310.1

ORF Insert Sequence:

ATGGGGAAGT CCGAAGGCCC AGTGGGGATG GTGGAGAGCG CCGGCCGTGC AGGGCAGAAG 
CGCCCGGGGT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TGCTGGTGAC CGCTGCCCTG 
GTGGCCTTGG GTGTCCTCTA CGCCGACCGC AGAGGGAAGC AGCTGCCACG CCTTGCTAGC 
CGGCTGTGCT TCTTACAGGA GGAGAGGACC TTTGTAAAAC GAAAACCCCG AGGGATCCCA 
GAGGCCCAAG AGGTGAGCGA GGTCTGCACC ACCCCTGGCT GCGTGATAGC AGCTGCCAGG 
ATCCTCCAGA ACATGGACCC GACCACGGAA CCGTGTGACG ACTTCTACCA GTTTGCATGC 
GGAGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CAAGATACAG CATCTTTGAC 
GTCCTCCGCG ACGAGCTGGA GGTCATCCTC AAAGCGGTGC TGGAGAATTC GACTGCCAAG 
GACCGGCCGG CTGTGGAGAA GGCCAGGACG CTGTACCGCT CCTGCATGAA CCAGAGTGTG 
ATAGAGAAGC GAGGCTCTCA GCCCCTGCTG GACATCTTGG AGGTGGTGGG AGGCTGGCCG 
GTGGCGATGG ACAGGTGGAA CGAGACCGTA GGACTCGAGT GGGAGCTGGA GCGGCAGCTG 
GCGCTGATGA ACTCACAGTT CAACAGGCGC GTCCTCATCG ACCTCTTCAT CTGGAACGAC 
GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAGC CCACCTTGGG CATGCCCTCC 
CGAGAGTACT ACTTCAACGG CGGCAGCAAC CGGAAGGTGC GGGAAGCCTA CCTGCAGTTC 
ATGGTGTCAG TGGCCACGTT GCTGCGGGAG GATGCAAACC TGCCCAGGGA CAGCTGCCTG 
GTGCAGGAGG ACATGGTGCA GGTGCTGGAG CTGGAGACAC AGCTGGCCAA GGCCACGGTA 
CCCCAGGAGG AGAGACACGA CGTCATCGCC TTGTACCACC GGATGGGACT GGAGGAGCTG 
CAAAGCCAGT TTGGCCTGAA GGGATTTAAC TGGACTCTGT TCATACAAAC TGTGCTATCC 
TCTGTCAAAA TCAAGCTGCT GCCAGATGAG GAAGTGGTGG TCTATGGCAT CCCCTACCTG 
CAGAACCTTG AAAACATCAT CGACACCTAC TCAGCCAGGA CCATACAGAA CTACCTGGTC 
TGGCGCCTGG TGCTGGACCG CATTGGTAGC CTAAGCCAGA GATTCAAGGA CACACGAGTG 
AACTACCGCA AGGCGCTGTT TGGCACAATG GTGGAGGAGG TGCGCTGGCG TGAATGTGTG 
GGCTACGTCA ACAGCAACAT GGAGAACGCC GTGGGCTCCC TCTACGTCAG GGAGGCGTTC 
CCTGGAGACA GCAAGAGCAT GGTCAGAGAA CTCATTGACA AGGTGCGGAC AGTGTTTGTG 
GAGACGCTGG ACGAGCTGGG CTGGATGGAC GAGGAGTCCA AGAAGAAGGC GCAGGAGAAG 
GCCATGAGCA TCCGGGAGCA GATCGGGCAC CCTGACTACA TCCTGGAGGA GATGAACAGG 
CGCCTGGACG AGGAGTACTC CAATCTGAAC TTCTCAGAGG ACCTGTACTT TGAGAACAGT 
CTGCAGAACC TCAAGGTGGG CGCCCAGCGG AGCCTCAGGA AGCTTCGGGA AAAGGTGGAC 
CCAAATCTCT GGATCATCGG GGCGGCGGTG GTCAATGCGT TCTACTCCCC AAACCGAAAC 
CAGATTGTAT TCCCTGCCGG GATCCTCCAG CCCCCCTTCT TCAGCAAGGA GCAGCCACAG 
GCCTTGAACT TTGGAGGCAT TGGGATGGTG ATCGGGCACG AGATCACGCA CGGCTTTGAC 
GACAATGGCC GGAACTTCGA CAAGAATGGC AACATGATGG ATTGGTGGAG TAACTTCTCC 
ACCCAGCACT TCCGGGAGCA GTCAGAGTGC ATGATCTACC AGTACGGCAA CTACTCCTGG 
GACCTGGCAG ACGAACAGAA CGTGAACGGA TTCAACACCC TTGGGGAAAA CATTGCTGAC 
AACGGAGGGG TGCGGCAAGC CTATAAGGCC TACCTCAAGT GGATGGCAGA GGGTGGCAAG 
GACCAGCAGC TGCCCGGCCT GGATCTCACC CATGAGCAGC TCTTCTTCAT CAACTATGCC 
CAGGTGTGGT GCGGGTCCTA CCGGCCCGAG TTCGCCATCC AATCCATCAA GACAGACGTC 
CACAGTCCCC TGAAGTACAG GGTACTGGGG TCGCTGCAGA ACCTGGCCGC CTTCGCAGAC 
ACGTTCCACT GTGCCCGGGG CACCCCCATG CACCCCAAGG AGCGATGCCG CGTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMEL1 ( XM_011542122.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540424.1 Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X8, mRNA.

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CloneID	OHu71833
Clone ID Related Accession (Same CDS sequence)	XM_011542122.2
Accession Version	XM_011542122.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	membrane metallo-endopeptidase-like 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542122.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCC GAGAGTACTA CTTCAACGGC GGCAGCAACC GGAAGGTGCG GGAAGCCTAC 
CTGCAGTTCA TGGTGTCAGT GGCCACGTTG CTGCGGGAGG ATGCAAACCT GCCCAGGGAC 
AGCTGCCTGG TGCAGGAGGA CATGGTGCAG GTGCTGGAGC TGGAGACACA GCTGGCCAAG 
GCCACGGTAC CCCAGGAGGA GAGACACGAC GTCATCGCCT TGTACCACCG GATGGGACTG 
GAGGAGCTGC AAAGCCAGTT TGGCCTGAAG GGATTTAACT GGACTCTGTT CATACAAACT 
GTGCTATCCT CTGTCAAAAT CAAGCTGCTG CCAGATGAGG AAGTGGTGGT CTATGGCATC 
CCCTACCTGC AGAACCTTGA AAACATCATC GACACCTACT CAGCCAGGAC CATACAGAAC 
TACCTGGTCT GGCGCCTGGT GCTGGACCGC ATTGGTAGCC TAAGCCAGAG ATTCAAGGAC 
ACACGAGTGA ACTACCGCAA GGCGCTGTTT GGCACAATGG TGGAGGAGGT GCGCTGGCGT 
GAATGTGTGG GCTACGTCAA CAGCAACATG GAGAACGCCG TGGGCTCCCT CTACGTCAGG 
GAGGCGTTCC CTGGAGACAG CAAGAGCATG GTCAGAGAAC TCATTGACAA GGTGCGGACA 
GTGTTTGTGG AGACGCTGGA CGAGCTGGGC TGGATGGACG AGGAGTCCAA GAAGAAGGCG 
CAGGAGAAGG CCATGAGCAT CCGGGAGCAG ATCGGGCACC CTGACTACAT CCTGGAGGAG 
ATGAACAGGC GCCTGGACGA GGAGTACTCC AATCTGAACT TCTCAGAGGA CCTGTACTTT 
GAGAACAGTC TGCAGAACCT CAAGGTGGGC GCCCAGCGGA GCCTCAGGAA GCTTCGGGAA 
AAGGTGGACC CAAATCTCTG GATCATCGGG GCGGCGGTGG TCAATGCGTT CTACTCCCCA 
AACCGAAACC AGATTGTATT CCCTGCCGGG ATCCTCCAGC CCCCCTTCTT CAGCAAGGAG 
CAGCCACAGG CCTTGAACTT TGGAGGCATT GGGATGGTGA TCGGGCACGA GATCACGCAC 
GGCTTTGACG ACAATGGCCG GAACTTCGAC AAGAATGGCA ACATGATGGA TTGGTGGAGT 
AACTTCTCCA CCCAGCACTT CCGGGAGCAG TCAGAGTGCA TGATCTACCA GTACGGCAAC 
TACTCCTGGG ACCTGGCAGA CGAACAGAAC GTGAACGGAT TCAACACCCT TGGGGAAAAC 
ATTGCTGACA ACGGAGGGGT GCGGCAAGCC TATAAGGCCT ACCTCAAGTG GATGGCAGAG 
GGTGGCAAGG ACCAGCAGCT GCCCGGCCTG GATCTCACCC ATGAGCAGCT CTTCTTCATC 
AACTATGCCC AGGTGTGGTG CGGGTCCTAC CGGCCCGAGT TCGCCATCCA ATCCATCAAG 
ACAGACGTCC ACAGTCCCCT GAAGTACAGG GTACTGGGGT CGCTGCAGAA CCTGGCCGCC 
TTCGCAGACA CGTTCCACTG TGCCCGGGGC ACCCCCATGC ACCCCAAGGA GCGATGCCGC 
GTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540424.1
CDS	89..1657
Translation	MPSREYYFNGGSNRKVREAYLQFMVSVATLLREDANLPRDSCLVQEDMVQVLELETQLAKATVPQEERHDVIALYHRMGLEELQSQFGLKGFNWTLFIQTVLSSVKIKLLPDEEVVVYGIPYLQNLENIIDTYSARTIQNYLVWRLVLDRIGSLSQRFKDTRVNYRKALFGTMVEEVRWRECVGYVNSNMENAVGSLYVREAFPGDSKSMVRELIDKVRTVFVETLDELGWMDEESKKKAQEKAMSIREQIGHPDYILEEMNRRLDEEYSNLNFSEDLYFENSLQNLKVGAQRSLRKLREKVDPNLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKEQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMMDWWSNFSTQHFREQSECMIYQYGNYSWDLADEQNVNGFNTLGENIADNGGVRQAYKAYLKWMAEGGKDQQLPGLDLTHEQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLAAFADTFHCARGTPMHPKERCRVW

Target ORF information:

RefSeq Version	XM_011542122.2
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542122.2

ORF Insert Sequence:

ATGCCCTCCC GAGAGTACTA CTTCAACGGC GGCAGCAACC GGAAGGTGCG GGAAGCCTAC 
CTGCAGTTCA TGGTGTCAGT GGCCACGTTG CTGCGGGAGG ATGCAAACCT GCCCAGGGAC 
AGCTGCCTGG TGCAGGAGGA CATGGTGCAG GTGCTGGAGC TGGAGACACA GCTGGCCAAG 
GCCACGGTAC CCCAGGAGGA GAGACACGAC GTCATCGCCT TGTACCACCG GATGGGACTG 
GAGGAGCTGC AAAGCCAGTT TGGCCTGAAG GGATTTAACT GGACTCTGTT CATACAAACT 
GTGCTATCCT CTGTCAAAAT CAAGCTGCTG CCAGATGAGG AAGTGGTGGT CTATGGCATC 
CCCTACCTGC AGAACCTTGA AAACATCATC GACACCTACT CAGCCAGGAC CATACAGAAC 
TACCTGGTCT GGCGCCTGGT GCTGGACCGC ATTGGTAGCC TAAGCCAGAG ATTCAAGGAC 
ACACGAGTGA ACTACCGCAA GGCGCTGTTT GGCACAATGG TGGAGGAGGT GCGCTGGCGT 
GAATGTGTGG GCTACGTCAA CAGCAACATG GAGAACGCCG TGGGCTCCCT CTACGTCAGG 
GAGGCGTTCC CTGGAGACAG CAAGAGCATG GTCAGAGAAC TCATTGACAA GGTGCGGACA 
GTGTTTGTGG AGACGCTGGA CGAGCTGGGC TGGATGGACG AGGAGTCCAA GAAGAAGGCG 
CAGGAGAAGG CCATGAGCAT CCGGGAGCAG ATCGGGCACC CTGACTACAT CCTGGAGGAG 
ATGAACAGGC GCCTGGACGA GGAGTACTCC AATCTGAACT TCTCAGAGGA CCTGTACTTT 
GAGAACAGTC TGCAGAACCT CAAGGTGGGC GCCCAGCGGA GCCTCAGGAA GCTTCGGGAA 
AAGGTGGACC CAAATCTCTG GATCATCGGG GCGGCGGTGG TCAATGCGTT CTACTCCCCA 
AACCGAAACC AGATTGTATT CCCTGCCGGG ATCCTCCAGC CCCCCTTCTT CAGCAAGGAG 
CAGCCACAGG CCTTGAACTT TGGAGGCATT GGGATGGTGA TCGGGCACGA GATCACGCAC 
GGCTTTGACG ACAATGGCCG GAACTTCGAC AAGAATGGCA ACATGATGGA TTGGTGGAGT 
AACTTCTCCA CCCAGCACTT CCGGGAGCAG TCAGAGTGCA TGATCTACCA GTACGGCAAC 
TACTCCTGGG ACCTGGCAGA CGAACAGAAC GTGAACGGAT TCAACACCCT TGGGGAAAAC 
ATTGCTGACA ACGGAGGGGT GCGGCAAGCC TATAAGGCCT ACCTCAAGTG GATGGCAGAG 
GGTGGCAAGG ACCAGCAGCT GCCCGGCCTG GATCTCACCC ATGAGCAGCT CTTCTTCATC 
AACTATGCCC AGGTGTGGTG CGGGTCCTAC CGGCCCGAGT TCGCCATCCA ATCCATCAAG 
ACAGACGTCC ACAGTCCCCT GAAGTACAGG GTACTGGGGT CGCTGCAGAA CCTGGCCGCC 
TTCGCAGACA CGTTCCACTG TGCCCGGGGC ACCCCCATGC ACCCCAAGGA GCGATGCCGC 
GTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMEL1 ( XM_017002315.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857804.1 Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu90514
Clone ID Related Accession (Same CDS sequence)	XM_017002315.1
Accession Version	XM_017002315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	membrane metallo-endopeptidase-like 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGA CCACGGAACC GTGTGACGAC TTCTACCAGT TTGCATGCGG AGGCTGGCTG 
CGGCGCCACG TGATCCCTGA GACCAACTCA AGATACAGCA TCTTTGACGT CCTCCGCGAC 
GAGCTGGAGG TCATCCTCAA AGCGGTGCTG GAGAATTCGA CTGCCAAGGA CCGGCCGGCT 
GTGGAGAAGG CCAGGACGCT GTACCGCTCC TGCATGAACC AGAGTGTGAT AGAGAAGCGA 
GGCTCTCAGC CCCTGCTGGA CATCTTGGAG GTGGTGGGAG GCTGGCCGGT GGCGATGGAC 
AGGTGGAACG AGACCGTAGG ACTCGAGTGG GAGCTGGAGC GGCAGCTGGC GCTGATGAAC 
TCACAGTTCA ACAGGCGCGT CCTCATCGAC CTCTTCATCT GGAACGACGA CCAGAACTCC 
AGCCGGCACA TCATCTACAT AGACCAGCCC ACCTTGGGCA TGCCCTCCCG AGAGTACTAC 
TTCAACGGCG GCAGCAACCG GAAGGTGCGG GAAGCCTACC TGCAGTTCAT GGTGTCAGTG 
GCCACGTTGC TGCGGGAGGA TGCAAACCTG CCCAGGGACA GCTGCCTGGT GCAGGAGGAC 
ATGGTGCAGG TGCTGGAGCT GGAGACACAG CTGGCCAAGG CCACGGTACC CCAGGAGGAG 
AGACACGACG TCATCGCCTT GTACCACCGG ATGGGACTGG AGGAGCTGCA AAGCCAGTTT 
GGCCTGAAGG GATTTAACTG GACTCTGTTC ATACAAACTG TGCTATCCTC TGTCAAAATC 
AAGCTGCTGC CAGATGAGGA AGTGGTGGTC TATGGCATCC CCTACCTGCA GAACCTTGAA 
AACATCATCG ACACCTACTC AGCCAGGACC ATACAGAACT ACCTGGTCTG GCGCCTGGTG 
CTGGACCGCA TTGGTAGCCT AAGCCAGAGA TTCAAGGACA CACGAGTGAA CTACCGCAAG 
GCGCTGTTTG GCACAATGGT GGAGGAGGTG CGCTGGCGTG AATGTGTGGG CTACGTCAAC 
AGCAACATGG AGAACGCCGT GGGCTCCCTC TACGTCAGGG AGGCGTTCCC TGGAGACAGC 
AAGAGCATGG TCAGAGAACT CATTGACAAG GTGCGGACAG TGTTTGTGGA GACGCTGGAC 
GAGCTGGGCT GGATGGACGA GGAGTCCAAG AAGAAGGCGC AGGAGAAGGC CATGAGCATC 
CGGGAGCAGA TCGGGCACCC TGACTACATC CTGGAGGAGA TGAACAGGCG CCTGGACGAG 
GAGTACTCCA ATCTGAACTT CTCAGAGGAC CTGTACTTTG AGAACAGTCT GCAGAACCTC 
AAGGTGGGCG CCCAGCGGAG CCTCAGGAAG CTTCGGGAAA AGGTGGACCC AAATCTCTGG 
ATCATCGGGG CGGCGGTGGT CAATGCGTTC TACTCCCCAA ACCGAAACCA GATTGTATTC 
CCTGCCGGGA TCCTCCAGCC CCCCTTCTTC AGCAAGGAGC AGCCACAGGC CTTGAACTTT 
GGAGGCATTG GGATGGTGAT CGGGCACGAG ATCACGCACG GCTTTGACGA CAATGGCCGG 
AACTTCGACA AGAATGGCAA CATGATGGAT TGGTGGAGTA ACTTCTCCAC CCAGCACTTC 
CGGGAGCAGT CAGAGTGCAT GATCTACCAG TACGGCAACT ACTCCTGGGA CCTGGCAGAC 
GAACAGAACG TGAACGGATT CAACACCCTT GGGGAAAACA TTGCTGACAA CGGAGGGGTG 
CGGCAAGCCT ATAAGGCCTA CCTCAAGTGG ATGGCAGAGG GTGGCAAGGA CCAGCAGCTG 
CCCGGCCTGG ATCTCACCCA TGAGCAGCTC TTCTTCATCA ACTATGCCCA GGTGTGGTGC 
GGGTCCTACC GGCCCGAGTT CGCCATCCAA TCCATCAAGA CAGACGTCCA CAGTCCCCTG 
AAGTACAGGG TACTGGGGTC GCTGCAGAAC CTGGCCGCCT TCGCAGACAC GTTCCACTGT 
GCCCGGGGCA CCCCCATGCA CCCCAAGGAG CGATGCCGCG TGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857804.1
CDS	403..2430
Translation	MDPTTEPCDDFYQFACGGWLRRHVIPETNSRYSIFDVLRDELEVILKAVLENSTAKDRPAVEKARTLYRSCMNQSVIEKRGSQPLLDILEVVGGWPVAMDRWNETVGLEWELERQLALMNSQFNRRVLIDLFIWNDDQNSSRHIIYIDQPTLGMPSREYYFNGGSNRKVREAYLQFMVSVATLLREDANLPRDSCLVQEDMVQVLELETQLAKATVPQEERHDVIALYHRMGLEELQSQFGLKGFNWTLFIQTVLSSVKIKLLPDEEVVVYGIPYLQNLENIIDTYSARTIQNYLVWRLVLDRIGSLSQRFKDTRVNYRKALFGTMVEEVRWRECVGYVNSNMENAVGSLYVREAFPGDSKSMVRELIDKVRTVFVETLDELGWMDEESKKKAQEKAMSIREQIGHPDYILEEMNRRLDEEYSNLNFSEDLYFENSLQNLKVGAQRSLRKLREKVDPNLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKEQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMMDWWSNFSTQHFREQSECMIYQYGNYSWDLADEQNVNGFNTLGENIADNGGVRQAYKAYLKWMAEGGKDQQLPGLDLTHEQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLAAFADTFHCARGTPMHPKERCRVW

Target ORF information:

RefSeq Version	XM_017002315.1
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002315.1

ORF Insert Sequence:

ATGGACCCGA CCACGGAACC GTGTGACGAC TTCTACCAGT TTGCATGCGG AGGCTGGCTG 
CGGCGCCACG TGATCCCTGA GACCAACTCA AGATACAGCA TCTTTGACGT CCTCCGCGAC 
GAGCTGGAGG TCATCCTCAA AGCGGTGCTG GAGAATTCGA CTGCCAAGGA CCGGCCGGCT 
GTGGAGAAGG CCAGGACGCT GTACCGCTCC TGCATGAACC AGAGTGTGAT AGAGAAGCGA 
GGCTCTCAGC CCCTGCTGGA CATCTTGGAG GTGGTGGGAG GCTGGCCGGT GGCGATGGAC 
AGGTGGAACG AGACCGTAGG ACTCGAGTGG GAGCTGGAGC GGCAGCTGGC GCTGATGAAC 
TCACAGTTCA ACAGGCGCGT CCTCATCGAC CTCTTCATCT GGAACGACGA CCAGAACTCC 
AGCCGGCACA TCATCTACAT AGACCAGCCC ACCTTGGGCA TGCCCTCCCG AGAGTACTAC 
TTCAACGGCG GCAGCAACCG GAAGGTGCGG GAAGCCTACC TGCAGTTCAT GGTGTCAGTG 
GCCACGTTGC TGCGGGAGGA TGCAAACCTG CCCAGGGACA GCTGCCTGGT GCAGGAGGAC 
ATGGTGCAGG TGCTGGAGCT GGAGACACAG CTGGCCAAGG CCACGGTACC CCAGGAGGAG 
AGACACGACG TCATCGCCTT GTACCACCGG ATGGGACTGG AGGAGCTGCA AAGCCAGTTT 
GGCCTGAAGG GATTTAACTG GACTCTGTTC ATACAAACTG TGCTATCCTC TGTCAAAATC 
AAGCTGCTGC CAGATGAGGA AGTGGTGGTC TATGGCATCC CCTACCTGCA GAACCTTGAA 
AACATCATCG ACACCTACTC AGCCAGGACC ATACAGAACT ACCTGGTCTG GCGCCTGGTG 
CTGGACCGCA TTGGTAGCCT AAGCCAGAGA TTCAAGGACA CACGAGTGAA CTACCGCAAG 
GCGCTGTTTG GCACAATGGT GGAGGAGGTG CGCTGGCGTG AATGTGTGGG CTACGTCAAC 
AGCAACATGG AGAACGCCGT GGGCTCCCTC TACGTCAGGG AGGCGTTCCC TGGAGACAGC 
AAGAGCATGG TCAGAGAACT CATTGACAAG GTGCGGACAG TGTTTGTGGA GACGCTGGAC 
GAGCTGGGCT GGATGGACGA GGAGTCCAAG AAGAAGGCGC AGGAGAAGGC CATGAGCATC 
CGGGAGCAGA TCGGGCACCC TGACTACATC CTGGAGGAGA TGAACAGGCG CCTGGACGAG 
GAGTACTCCA ATCTGAACTT CTCAGAGGAC CTGTACTTTG AGAACAGTCT GCAGAACCTC 
AAGGTGGGCG CCCAGCGGAG CCTCAGGAAG CTTCGGGAAA AGGTGGACCC AAATCTCTGG 
ATCATCGGGG CGGCGGTGGT CAATGCGTTC TACTCCCCAA ACCGAAACCA GATTGTATTC 
CCTGCCGGGA TCCTCCAGCC CCCCTTCTTC AGCAAGGAGC AGCCACAGGC CTTGAACTTT 
GGAGGCATTG GGATGGTGAT CGGGCACGAG ATCACGCACG GCTTTGACGA CAATGGCCGG 
AACTTCGACA AGAATGGCAA CATGATGGAT TGGTGGAGTA ACTTCTCCAC CCAGCACTTC 
CGGGAGCAGT CAGAGTGCAT GATCTACCAG TACGGCAACT ACTCCTGGGA CCTGGCAGAC 
GAACAGAACG TGAACGGATT CAACACCCTT GGGGAAAACA TTGCTGACAA CGGAGGGGTG 
CGGCAAGCCT ATAAGGCCTA CCTCAAGTGG ATGGCAGAGG GTGGCAAGGA CCAGCAGCTG 
CCCGGCCTGG ATCTCACCCA TGAGCAGCTC TTCTTCATCA ACTATGCCCA GGTGTGGTGC 
GGGTCCTACC GGCCCGAGTT CGCCATCCAA TCCATCAAGA CAGACGTCCA CAGTCCCCTG 
AAGTACAGGG TACTGGGGTC GCTGCAGAAC CTGGCCGCCT TCGCAGACAC GTTCCACTGT 
GCCCGGGGCA CCCCCATGCA CCCCAAGGAG CGATGCCGCG TGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMEL1 ( XM_017002311.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857800.1 Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu90510
Clone ID Related Accession (Same CDS sequence)	XM_017002311.1
Accession Version	XM_017002311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	membrane metallo-endopeptidase-like 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGT CCGAAGGCCC AGTGGGGATG GTGGAGAGCG CCGGCCGTGC AGGGCAGAAG 
CGCCCGGGGT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TGCTGGTGAC CGCTGCCCTG 
GTGGCCTTGG GTGTCCTCTA CGCCGACCGC AGAGGGAAGC AGCTGCCACG CCTTGCTAGC 
CGGCTGTGCT TCTTACAGGA GGAGAGGACC TTTGTAAAAC GAAAACCCCG AGGGATCCCA 
GAGGCCCAAG AGGTGAGCGA GGTCTGCACC ACCCCTGGCT GCGTGATAGC AGCTGCCAGG 
ATCCTCCAGA ACATGGACCC GACCACGGAA CCGTGTGACG ACTTCTACCA GTTTGCATGC 
GGAGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CAAGATACAG CATCTTTGAC 
GTCCTCCGCG ACGAGCTGGA GGTCATCCTC AAAGCGGTGC TGGAGAATTC GACTGCCAAG 
GACCGGCCGG CTGTGGAGAA GGCCAGGACG CTGTACCGCT CCTGCATGAA CCAGAGTGTG 
ATAGAGAAGC GAGGCTCTCA GCCCCTGCTG GACATCTTGG AGGTGGTGGG AGGCTGGCCG 
GTGGCGATGG ACAGGTGGAA CGAGACCGTA GGACTCGAGT GGGAGCTGGA GCGGCAGCTG 
GCGCTGATGA ACTCACAGTT CAACAGGCGC GTCCTCATCG ACCTCTTCAT CTGGAACGAC 
GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAGC CCACCTTGGG CATGCCCTCC 
CGAGAGTACT ACTTCAACGG CGGCAGCAAC CGGAAGGTGC GGGAAGCCTA CCTGCAGTTC 
ATGGTGTCAG TGGCCACGTT GCTGCGGGAG GATGCAAACC TGCCCAGGGA CAGCTGCCTG 
GTGCAGGAGG ACATGGTGCA GGTGCTGGAG CTGGAGACAC AGCTGGCCAA GGCCACGGTA 
CCCCAGGAGG AGAGACACGA CGTCATCGCC TTGTACCACC GGATGGGACT GGAGGAGCTG 
CAAAGCCAGT TTGGCCTGAA GCTGCTGCCA GATGAGGAAG TGGTGGTCTA TGGCATCCCC 
TACCTGCAGA ACCTTGAAAA CATCATCGAC ACCTACTCAG CCAGGACCAT ACAGAACTAC 
CTGGTCTGGC GCCTGGTGCT GGACCGCATT GGTAGCCTAA GCCAGAGATT CAAGGACACA 
CGAGTGAACT ACCGCAAGGC GCTGTTTGGC ACAATGGTGG AGGAGGTGCG CTGGCGTGAA 
TGTGTGGGCT ACGTCAACAG CAACATGGAG AACGCCGTGG GCTCCCTCTA CGTCAGGGAG 
GCGTTCCCTG GAGACAGCAA GAGCATGGTC AGAGAACTCA TTGACAAGGT GCGGACAGTG 
TTTGTGGAGA CGCTGGACGA GCTGGGCTGG ATGGACGAGG AGTCCAAGAA GAAGGCGCAG 
GAGAAGGCCA TGAGCATCCG GGAGCAGATC GGGCACCCTG ACTACATCCT GGAGGAGATG 
AACAGGCGCC TGGACGAGGA GTACTCCAAT CTGAACTTCT CAGAGGACCT GTACTTTGAG 
AACAGTCTGC AGAACCTCAA GGTGGGCGCC CAGCGGAGCC TCAGGAAGCT TCGGGAAAAG 
GTGGACCCAA ATCTCTGGAT CATCGGGGCG GCGGTGGTCA ATGCGTTCTA CTCCCCAAAC 
CGAAACCAGA TTGTATTCCC TGCCGGGATC CTCCAGCCCC CCTTCTTCAG CAAGGAGCAG 
CCACAGGCCT TGAACTTTGG AGGCATTGGG ATGGTGATCG GGCACGAGAT CACGCACGGC 
TTTGACGACA ATGGCCGGAA CTTCGACAAG AATGGCAACA TGATGGATTG GTGGAGTAAC 
TTCTCCACCC AGCACTTCCG GGAGCAGTCA GAGTGCATGA TCTACCAGTA CGGCAACTAC 
TCCTGGGACC TGGCAGACGA ACAGAACGTG AACGGATTCA ACACCCTTGG GGAAAACATT 
GCTGACAACG GAGGGGTGCG GCAAGCCTAT AAGGCCTACC TCAAGTGGAT GGCAGAGGGT 
GGCAAGGACC AGCAGCTGCC CGGCCTGGAT CTCACCCATG AGCAGCTCTT CTTCATCAAC 
TATGCCCAGG TGTGGTGCGG GTCCTACCGG CCCGAGTTCG CCATCCAATC CATCAAGACA 
GACGTCCACA GTCCCCTGAA GTACAGGGTA CTGGGGTCGC TGCAGAACCT GGCCGCCTTC 
GCAGACACGT TCCACTGTGC CCGGGGCACC CCCATGCACC CCAAGGAGCG ATGCCGCGTG 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857800.1
CDS	215..2500
Translation	MGKSEGPVGMVESAGRAGQKRPGFLEGGLLLLLLLVTAALVALGVLYADRRGKQLPRLASRLCFLQEERTFVKRKPRGIPEAQEVSEVCTTPGCVIAAARILQNMDPTTEPCDDFYQFACGGWLRRHVIPETNSRYSIFDVLRDELEVILKAVLENSTAKDRPAVEKARTLYRSCMNQSVIEKRGSQPLLDILEVVGGWPVAMDRWNETVGLEWELERQLALMNSQFNRRVLIDLFIWNDDQNSSRHIIYIDQPTLGMPSREYYFNGGSNRKVREAYLQFMVSVATLLREDANLPRDSCLVQEDMVQVLELETQLAKATVPQEERHDVIALYHRMGLEELQSQFGLKLLPDEEVVVYGIPYLQNLENIIDTYSARTIQNYLVWRLVLDRIGSLSQRFKDTRVNYRKALFGTMVEEVRWRECVGYVNSNMENAVGSLYVREAFPGDSKSMVRELIDKVRTVFVETLDELGWMDEESKKKAQEKAMSIREQIGHPDYILEEMNRRLDEEYSNLNFSEDLYFENSLQNLKVGAQRSLRKLREKVDPNLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKEQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMMDWWSNFSTQHFREQSECMIYQYGNYSWDLADEQNVNGFNTLGENIADNGGVRQAYKAYLKWMAEGGKDQQLPGLDLTHEQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLAAFADTFHCARGTPMHPKERCRVW

Target ORF information:

RefSeq Version	XM_017002311.1
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002311.1

ORF Insert Sequence:

ATGGGGAAGT CCGAAGGCCC AGTGGGGATG GTGGAGAGCG CCGGCCGTGC AGGGCAGAAG 
CGCCCGGGGT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TGCTGGTGAC CGCTGCCCTG 
GTGGCCTTGG GTGTCCTCTA CGCCGACCGC AGAGGGAAGC AGCTGCCACG CCTTGCTAGC 
CGGCTGTGCT TCTTACAGGA GGAGAGGACC TTTGTAAAAC GAAAACCCCG AGGGATCCCA 
GAGGCCCAAG AGGTGAGCGA GGTCTGCACC ACCCCTGGCT GCGTGATAGC AGCTGCCAGG 
ATCCTCCAGA ACATGGACCC GACCACGGAA CCGTGTGACG ACTTCTACCA GTTTGCATGC 
GGAGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CAAGATACAG CATCTTTGAC 
GTCCTCCGCG ACGAGCTGGA GGTCATCCTC AAAGCGGTGC TGGAGAATTC GACTGCCAAG 
GACCGGCCGG CTGTGGAGAA GGCCAGGACG CTGTACCGCT CCTGCATGAA CCAGAGTGTG 
ATAGAGAAGC GAGGCTCTCA GCCCCTGCTG GACATCTTGG AGGTGGTGGG AGGCTGGCCG 
GTGGCGATGG ACAGGTGGAA CGAGACCGTA GGACTCGAGT GGGAGCTGGA GCGGCAGCTG 
GCGCTGATGA ACTCACAGTT CAACAGGCGC GTCCTCATCG ACCTCTTCAT CTGGAACGAC 
GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAGC CCACCTTGGG CATGCCCTCC 
CGAGAGTACT ACTTCAACGG CGGCAGCAAC CGGAAGGTGC GGGAAGCCTA CCTGCAGTTC 
ATGGTGTCAG TGGCCACGTT GCTGCGGGAG GATGCAAACC TGCCCAGGGA CAGCTGCCTG 
GTGCAGGAGG ACATGGTGCA GGTGCTGGAG CTGGAGACAC AGCTGGCCAA GGCCACGGTA 
CCCCAGGAGG AGAGACACGA CGTCATCGCC TTGTACCACC GGATGGGACT GGAGGAGCTG 
CAAAGCCAGT TTGGCCTGAA GCTGCTGCCA GATGAGGAAG TGGTGGTCTA TGGCATCCCC 
TACCTGCAGA ACCTTGAAAA CATCATCGAC ACCTACTCAG CCAGGACCAT ACAGAACTAC 
CTGGTCTGGC GCCTGGTGCT GGACCGCATT GGTAGCCTAA GCCAGAGATT CAAGGACACA 
CGAGTGAACT ACCGCAAGGC GCTGTTTGGC ACAATGGTGG AGGAGGTGCG CTGGCGTGAA 
TGTGTGGGCT ACGTCAACAG CAACATGGAG AACGCCGTGG GCTCCCTCTA CGTCAGGGAG 
GCGTTCCCTG GAGACAGCAA GAGCATGGTC AGAGAACTCA TTGACAAGGT GCGGACAGTG 
TTTGTGGAGA CGCTGGACGA GCTGGGCTGG ATGGACGAGG AGTCCAAGAA GAAGGCGCAG 
GAGAAGGCCA TGAGCATCCG GGAGCAGATC GGGCACCCTG ACTACATCCT GGAGGAGATG 
AACAGGCGCC TGGACGAGGA GTACTCCAAT CTGAACTTCT CAGAGGACCT GTACTTTGAG 
AACAGTCTGC AGAACCTCAA GGTGGGCGCC CAGCGGAGCC TCAGGAAGCT TCGGGAAAAG 
GTGGACCCAA ATCTCTGGAT CATCGGGGCG GCGGTGGTCA ATGCGTTCTA CTCCCCAAAC 
CGAAACCAGA TTGTATTCCC TGCCGGGATC CTCCAGCCCC CCTTCTTCAG CAAGGAGCAG 
CCACAGGCCT TGAACTTTGG AGGCATTGGG ATGGTGATCG GGCACGAGAT CACGCACGGC 
TTTGACGACA ATGGCCGGAA CTTCGACAAG AATGGCAACA TGATGGATTG GTGGAGTAAC 
TTCTCCACCC AGCACTTCCG GGAGCAGTCA GAGTGCATGA TCTACCAGTA CGGCAACTAC 
TCCTGGGACC TGGCAGACGA ACAGAACGTG AACGGATTCA ACACCCTTGG GGAAAACATT 
GCTGACAACG GAGGGGTGCG GCAAGCCTAT AAGGCCTACC TCAAGTGGAT GGCAGAGGGT 
GGCAAGGACC AGCAGCTGCC CGGCCTGGAT CTCACCCATG AGCAGCTCTT CTTCATCAAC 
TATGCCCAGG TGTGGTGCGG GTCCTACCGG CCCGAGTTCG CCATCCAATC CATCAAGACA 
GACGTCCACA GTCCCCTGAA GTACAGGGTA CTGGGGTCGC TGCAGAACCT GGCCGCCTTC 
GCAGACACGT TCCACTGTGC CCGGGGCACC CCCATGCACC CCAAGGAGCG ATGCCGCGTG 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMEL1 ( XM_017002312.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857801.1 Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu90511
Clone ID Related Accession (Same CDS sequence)	XM_017002312.1
Accession Version	XM_017002312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	membrane metallo-endopeptidase-like 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGT CCGAAGGCCC AGTGGGGATG GTGGAGAGCG CCGGCCGTGC AGGGCAGAAG 
CGCCCGGGGT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TGCTGGTGAC CGCTGCCCTG 
GTGGCCTTGG GTGTCCTCTA CGCCGACCGC AGAGGGATCC CAGAGGCCCA AGAGGTGAGC 
GAGGTCTGCA CCACCCCTGG CTGCGTGATA GCAGCTGCCA GGATCCTCCA GAACATGGAC 
CCGACCACGG AACCGTGTGA CGACTTCTAC CAGTTTGCAT GCGGAGGCTG GCTGCGGCGC 
CACGTGATCC CTGAGACCAA CTCAAGATAC AGCATCTTTG ACGTCCTCCG CGACGAGCTG 
GAGGTCATCC TCAAAGCGGT GCTGGAGAAT TCGACTGCCA AGGACCGGCC GGCTGTGGAG 
AAGGCCAGGA CGCTGTACCG CTCCTGCATG AACCAGAGTG TGATAGAGAA GCGAGGCTCT 
CAGCCCCTGC TGGACATCTT GGAGGTGGTG GGAGGCTGGC CGGTGGCGAT GGACAGGTGG 
AACGAGACCG TAGGACTCGA GTGGGAGCTG GAGCGGCAGC TGGCGCTGAT GAACTCACAG 
TTCAACAGGC GCGTCCTCAT CGACCTCTTC ATCTGGAACG ACGACCAGAA CTCCAGCCGG 
CACATCATCT ACATAGACCA GCCCACCTTG GGCATGCCCT CCCGAGAGTA CTACTTCAAC 
GGCGGCAGCA ACCGGAAGGT GCGGGAAGCC TACCTGCAGT TCATGGTGTC AGTGGCCACG 
TTGCTGCGGG AGGATGCAAA CCTGCCCAGG GACAGCTGCC TGGTGCAGGA GGACATGGTG 
CAGGTGCTGG AGCTGGAGAC ACAGCTGGCC AAGGCCACGG TACCCCAGGA GGAGAGACAC 
GACGTCATCG CCTTGTACCA CCGGATGGGA CTGGAGGAGC TGCAAAGCCA GTTTGGCCTG 
AAGGGATTTA ACTGGACTCT GTTCATACAA ACTGTGCTAT CCTCTGTCAA AATCAAGCTG 
CTGCCAGATG AGGAAGTGGT GGTCTATGGC ATCCCCTACC TGCAGAACCT TGAAAACATC 
ATCGACACCT ACTCAGCCAG GACCATACAG AACTACCTGG TCTGGCGCCT GGTGCTGGAC 
CGCATTGGTA GCCTAAGCCA GAGATTCAAG GACACACGAG TGAACTACCG CAAGGCGCTG 
TTTGGCACAA TGGTGGAGGA GGTGCGCTGG CGTGAATGTG TGGGCTACGT CAACAGCAAC 
ATGGAGAACG CCGTGGGCTC CCTCTACGTC AGGGAGGCGT TCCCTGGAGA CAGCAAGAGC 
ATGGTCAGAG AACTCATTGA CAAGGTGCGG ACAGTGTTTG TGGAGACGCT GGACGAGCTG 
GGCTGGATGG ACGAGGAGTC CAAGAAGAAG GCGCAGGAGA AGGCCATGAG CATCCGGGAG 
CAGATCGGGC ACCCTGACTA CATCCTGGAG GAGATGAACA GGCGCCTGGA CGAGGAGTAC 
TCCAATCTGA ACTTCTCAGA GGACCTGTAC TTTGAGAACA GTCTGCAGAA CCTCAAGGTG 
GGCGCCCAGC GGAGCCTCAG GAAGCTTCGG GAAAAGGTGG ACCCAAATCT CTGGATCATC 
GGGGCGGCGG TGGTCAATGC GTTCTACTCC CCAAACCGAA ACCAGATTGT ATTCCCTGCC 
GGGATCCTCC AGCCCCCCTT CTTCAGCAAG GAGCAGCCAC AGGCCTTGAA CTTTGGAGGC 
ATTGGGATGG TGATCGGGCA CGAGATCACG CACGGCTTTG ACGACAATGG CCGGAACTTC 
GACAAGAATG GCAACATGAT GGATTGGTGG AGTAACTTCT CCACCCAGCA CTTCCGGGAG 
CAGTCAGAGT GCATGATCTA CCAGTACGGC AACTACTCCT GGGACCTGGC AGACGAACAG 
AACGTGAACG GATTCAACAC CCTTGGGGAA AACATTGCTG ACAACGGAGG GGTGCGGCAA 
GCCTATAAGG CCTACCTCAA GTGGATGGCA GAGGGTGGCA AGGACCAGCA GCTGCCCGGC 
CTGGATCTCA CCCATGAGCA GCTCTTCTTC ATCAACTATG CCCAGGTGTG GTGCGGGTCC 
TACCGGCCCG AGTTCGCCAT CCAATCCATC AAGACAGACG TCCACAGTCC CCTGAAGTAC 
AGGGTACTGG GGTCGCTGCA GAACCTGGCC GCCTTCGCAG ACACGTTCCA CTGTGCCCGG 
GGCACCCCCA TGCACCCCAA GGAGCGATGC CGCGTGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857801.1
CDS	211..2472
Translation	MGKSEGPVGMVESAGRAGQKRPGFLEGGLLLLLLLVTAALVALGVLYADRRGIPEAQEVSEVCTTPGCVIAAARILQNMDPTTEPCDDFYQFACGGWLRRHVIPETNSRYSIFDVLRDELEVILKAVLENSTAKDRPAVEKARTLYRSCMNQSVIEKRGSQPLLDILEVVGGWPVAMDRWNETVGLEWELERQLALMNSQFNRRVLIDLFIWNDDQNSSRHIIYIDQPTLGMPSREYYFNGGSNRKVREAYLQFMVSVATLLREDANLPRDSCLVQEDMVQVLELETQLAKATVPQEERHDVIALYHRMGLEELQSQFGLKGFNWTLFIQTVLSSVKIKLLPDEEVVVYGIPYLQNLENIIDTYSARTIQNYLVWRLVLDRIGSLSQRFKDTRVNYRKALFGTMVEEVRWRECVGYVNSNMENAVGSLYVREAFPGDSKSMVRELIDKVRTVFVETLDELGWMDEESKKKAQEKAMSIREQIGHPDYILEEMNRRLDEEYSNLNFSEDLYFENSLQNLKVGAQRSLRKLREKVDPNLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKEQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMMDWWSNFSTQHFREQSECMIYQYGNYSWDLADEQNVNGFNTLGENIADNGGVRQAYKAYLKWMAEGGKDQQLPGLDLTHEQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLAAFADTFHCARGTPMHPKERCRVW

Target ORF information:

RefSeq Version	XM_017002312.1
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002312.1

ORF Insert Sequence:

ATGGGGAAGT CCGAAGGCCC AGTGGGGATG GTGGAGAGCG CCGGCCGTGC AGGGCAGAAG 
CGCCCGGGGT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TGCTGGTGAC CGCTGCCCTG 
GTGGCCTTGG GTGTCCTCTA CGCCGACCGC AGAGGGATCC CAGAGGCCCA AGAGGTGAGC 
GAGGTCTGCA CCACCCCTGG CTGCGTGATA GCAGCTGCCA GGATCCTCCA GAACATGGAC 
CCGACCACGG AACCGTGTGA CGACTTCTAC CAGTTTGCAT GCGGAGGCTG GCTGCGGCGC 
CACGTGATCC CTGAGACCAA CTCAAGATAC AGCATCTTTG ACGTCCTCCG CGACGAGCTG 
GAGGTCATCC TCAAAGCGGT GCTGGAGAAT TCGACTGCCA AGGACCGGCC GGCTGTGGAG 
AAGGCCAGGA CGCTGTACCG CTCCTGCATG AACCAGAGTG TGATAGAGAA GCGAGGCTCT 
CAGCCCCTGC TGGACATCTT GGAGGTGGTG GGAGGCTGGC CGGTGGCGAT GGACAGGTGG 
AACGAGACCG TAGGACTCGA GTGGGAGCTG GAGCGGCAGC TGGCGCTGAT GAACTCACAG 
TTCAACAGGC GCGTCCTCAT CGACCTCTTC ATCTGGAACG ACGACCAGAA CTCCAGCCGG 
CACATCATCT ACATAGACCA GCCCACCTTG GGCATGCCCT CCCGAGAGTA CTACTTCAAC 
GGCGGCAGCA ACCGGAAGGT GCGGGAAGCC TACCTGCAGT TCATGGTGTC AGTGGCCACG 
TTGCTGCGGG AGGATGCAAA CCTGCCCAGG GACAGCTGCC TGGTGCAGGA GGACATGGTG 
CAGGTGCTGG AGCTGGAGAC ACAGCTGGCC AAGGCCACGG TACCCCAGGA GGAGAGACAC 
GACGTCATCG CCTTGTACCA CCGGATGGGA CTGGAGGAGC TGCAAAGCCA GTTTGGCCTG 
AAGGGATTTA ACTGGACTCT GTTCATACAA ACTGTGCTAT CCTCTGTCAA AATCAAGCTG 
CTGCCAGATG AGGAAGTGGT GGTCTATGGC ATCCCCTACC TGCAGAACCT TGAAAACATC 
ATCGACACCT ACTCAGCCAG GACCATACAG AACTACCTGG TCTGGCGCCT GGTGCTGGAC 
CGCATTGGTA GCCTAAGCCA GAGATTCAAG GACACACGAG TGAACTACCG CAAGGCGCTG 
TTTGGCACAA TGGTGGAGGA GGTGCGCTGG CGTGAATGTG TGGGCTACGT CAACAGCAAC 
ATGGAGAACG CCGTGGGCTC CCTCTACGTC AGGGAGGCGT TCCCTGGAGA CAGCAAGAGC 
ATGGTCAGAG AACTCATTGA CAAGGTGCGG ACAGTGTTTG TGGAGACGCT GGACGAGCTG 
GGCTGGATGG ACGAGGAGTC CAAGAAGAAG GCGCAGGAGA AGGCCATGAG CATCCGGGAG 
CAGATCGGGC ACCCTGACTA CATCCTGGAG GAGATGAACA GGCGCCTGGA CGAGGAGTAC 
TCCAATCTGA ACTTCTCAGA GGACCTGTAC TTTGAGAACA GTCTGCAGAA CCTCAAGGTG 
GGCGCCCAGC GGAGCCTCAG GAAGCTTCGG GAAAAGGTGG ACCCAAATCT CTGGATCATC 
GGGGCGGCGG TGGTCAATGC GTTCTACTCC CCAAACCGAA ACCAGATTGT ATTCCCTGCC 
GGGATCCTCC AGCCCCCCTT CTTCAGCAAG GAGCAGCCAC AGGCCTTGAA CTTTGGAGGC 
ATTGGGATGG TGATCGGGCA CGAGATCACG CACGGCTTTG ACGACAATGG CCGGAACTTC 
GACAAGAATG GCAACATGAT GGATTGGTGG AGTAACTTCT CCACCCAGCA CTTCCGGGAG 
CAGTCAGAGT GCATGATCTA CCAGTACGGC AACTACTCCT GGGACCTGGC AGACGAACAG 
AACGTGAACG GATTCAACAC CCTTGGGGAA AACATTGCTG ACAACGGAGG GGTGCGGCAA 
GCCTATAAGG CCTACCTCAA GTGGATGGCA GAGGGTGGCA AGGACCAGCA GCTGCCCGGC 
CTGGATCTCA CCCATGAGCA GCTCTTCTTC ATCAACTATG CCCAGGTGTG GTGCGGGTCC 
TACCGGCCCG AGTTCGCCAT CCAATCCATC AAGACAGACG TCCACAGTCC CCTGAAGTAC 
AGGGTACTGG GGTCGCTGCA GAACCTGGCC GCCTTCGCAG ACACGTTCCA CTGTGCCCGG 
GGCACCCCCA TGCACCCCAA GGAGCGATGC CGCGTGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMEL1 ( XM_017002313.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857802.1 Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu90512
Clone ID Related Accession (Same CDS sequence)	XM_017002313.1
Accession Version	XM_017002313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	membrane metallo-endopeptidase-like 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGT CCGAAGGCCC AGTGGGGATG GTGGAGAGCG CCGGCCGTGC AGGGCAGAAG 
CGCCCGGGGT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TGCTGGTGAC CGCTGCCCTG 
GTGGCCTTGG GTGTCCTCTA CGCCGACCGC AGAGGGAAGC AGCTGCCACG CCTTGCTAGC 
CGGCTGTGCT TCTTACAGGA GGAGAGGACC TTTGTAAAAC GAAAACCCCG AGGGATCCCA 
GAGGCCCAAG AGGTGAGCGA GGTCTGCACC ACCCCTGGCT GCGTGATAGC AGCTGCCAGG 
ATCCTCCAGA ACATGGACCC GACCACGGAA CCGTGTGACG ACTTCTACCA GTTTGCATGC 
GGAGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CAAGATACAG CATCTTTGAC 
GTCCTCCGCG ACGAGCTGGA GGTCATCCTC AAAGCGGTGC TGGAGAATTC GACTGCCAAG 
GACCGGCCGG CTGTGGAGAA GGCCAGGACG CTGTACCGCT CCTGCATGAA CCAGAGTGTG 
ATAGAGAAGC GAGGCTCTCA GCCCCTGCTG GACATCTTGG AGGTGGTGGG AGGCTGGCCG 
GTGGCGATGG ACAGGTGGAA CGAGACCGTA GGACTCGAGT GGGAGCTGGA GCGGCAGCTG 
GCGCTGATGA ACTCACAGTT CAACAGGCGC GTCCTCATCG ACCTCTTCAT CTGGAACGAC 
GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAGC CCACCTTGGG CATGCCCTCC 
CGAGAGTACT ACTTCAACGG CGGCAGCAAC CGGAAGGTGC GGGAAGCCTA CCTGCAGTTC 
ATGGTGTCAG TGGCCACGTT GCTGCGGGAG GATGCAAACC TGCCCAGGGA CAGCTGCCTG 
GTGCAGGAGG ACATGGTGCA GGTGCTGGAG CTGGAGACAC AGCTGGCCAA GGCCACGGTA 
CCCCAGGAGG AGAGACACGA CGTCATCGCC TTGTACCACC GGATGGGACT GGAGGAGCTG 
CAAAGCCAGT TTGGCCTGAA GGGATTTAAC TGGACTCTGT TCATACAAAC TGTGCTATCC 
TCTGTCAAAA TCAAGCTGCT GCCAGATGAG GAAGTGGTGG TCTATGGCAT CCCCTACCTG 
CAGAACCTTG AAAACATCAT CGACACCTAC TCAGCCAGGA CCATACAGAA CTACCTGGTC 
TGGCGCCTGG TGCTGGACCG CATTGGTAGC CTAAGCCAGA GATTCAAGGA CACACGAGTG 
AACTACCGCA AGGCGCTGTT TGGCACAATG GTGGAGGAGG TGCGCTGGCG TGAATGTGTG 
GGCTACGTCA ACAGCAACAT GGAGAACGCC GTGGGCTCCC TCTACGTCAG GGAGGCGTTC 
CCTGGAGACA GCAAGAGCAT GGTCAGAGAA CTCATTGACA AGGTGCGGAC AGTGTTTGTG 
GAGACGCTGG ACGAGCTGGG CTGGATGGAC GAGGAGTCCA AGAAGAAGGC GCAGGAGAAG 
GCCATGAGCA TCCGGGAGCA GATCGGGCAC CCTGACTACA TCCTGGAGGA GATGAACAGG 
CGCCTGGACG AGGAGTACTC CAATCTGAAC TTCTCAGAGG ACCTGTACTT TGAGAACAGT 
CTGCAGAACC TCAAGGTGGG CGCCCAGCGG AGCCTCAGGA AGCTTCGGGA AAAGGTGGAC 
CCAAATCTCT GGATCATCGG GGCGGCGGTG GTCAATGCGT TCTACTCCCC AAACCGAAAC 
CAGATTGTAT TCCCTGCCGG GATCCTCCAG CCCCCCTTCT TCAGCAAGGA GCAGCCACAG 
GCCTTGAACT TTGGAGGCAT TGGGATGGTG ATCGGGCACG AGATCACGCA CGGCTTTGAC 
GACAATGGCC GGAACTTCGA CAAGAATGGC AACATGATGG ATTGGTGGAG TAACTTCTCC 
ACCCAGCACT TCCGGGAGCA GTCAGAGTGC ATGATCTACC AGTACGGCAA CTACTCCTGG 
GACCTGGCAG ACGAACAGAA CGTGAACGGA TTCAACACCC TTGGGGAAAA CATTGCTGAC 
AACGGAGGGG TGCGGCAAGC CTATAAGGTG TGGTGCGGGT CCTACCGGCC CGAGTTCGCC 
ATCCAATCCA TCAAGACAGA CGTCCACAGT CCCCTGAAGT ACAGGGTACT GGGGTCGCTG 
CAGAACCTGG CCGCCTTCGC AGACACGTTC CACTGTGCCC GGGGCACCCC CATGCACCCC 
AAGGAGCGAT GCCGCGTGTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857802.1
CDS	215..2458
Translation	MGKSEGPVGMVESAGRAGQKRPGFLEGGLLLLLLLVTAALVALGVLYADRRGKQLPRLASRLCFLQEERTFVKRKPRGIPEAQEVSEVCTTPGCVIAAARILQNMDPTTEPCDDFYQFACGGWLRRHVIPETNSRYSIFDVLRDELEVILKAVLENSTAKDRPAVEKARTLYRSCMNQSVIEKRGSQPLLDILEVVGGWPVAMDRWNETVGLEWELERQLALMNSQFNRRVLIDLFIWNDDQNSSRHIIYIDQPTLGMPSREYYFNGGSNRKVREAYLQFMVSVATLLREDANLPRDSCLVQEDMVQVLELETQLAKATVPQEERHDVIALYHRMGLEELQSQFGLKGFNWTLFIQTVLSSVKIKLLPDEEVVVYGIPYLQNLENIIDTYSARTIQNYLVWRLVLDRIGSLSQRFKDTRVNYRKALFGTMVEEVRWRECVGYVNSNMENAVGSLYVREAFPGDSKSMVRELIDKVRTVFVETLDELGWMDEESKKKAQEKAMSIREQIGHPDYILEEMNRRLDEEYSNLNFSEDLYFENSLQNLKVGAQRSLRKLREKVDPNLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKEQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMMDWWSNFSTQHFREQSECMIYQYGNYSWDLADEQNVNGFNTLGENIADNGGVRQAYKVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLAAFADTFHCARGTPMHPKERCRVW

Target ORF information:

RefSeq Version	XM_017002313.1
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002313.1

ORF Insert Sequence:

ATGGGGAAGT CCGAAGGCCC AGTGGGGATG GTGGAGAGCG CCGGCCGTGC AGGGCAGAAG 
CGCCCGGGGT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TGCTGGTGAC CGCTGCCCTG 
GTGGCCTTGG GTGTCCTCTA CGCCGACCGC AGAGGGAAGC AGCTGCCACG CCTTGCTAGC 
CGGCTGTGCT TCTTACAGGA GGAGAGGACC TTTGTAAAAC GAAAACCCCG AGGGATCCCA 
GAGGCCCAAG AGGTGAGCGA GGTCTGCACC ACCCCTGGCT GCGTGATAGC AGCTGCCAGG 
ATCCTCCAGA ACATGGACCC GACCACGGAA CCGTGTGACG ACTTCTACCA GTTTGCATGC 
GGAGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CAAGATACAG CATCTTTGAC 
GTCCTCCGCG ACGAGCTGGA GGTCATCCTC AAAGCGGTGC TGGAGAATTC GACTGCCAAG 
GACCGGCCGG CTGTGGAGAA GGCCAGGACG CTGTACCGCT CCTGCATGAA CCAGAGTGTG 
ATAGAGAAGC GAGGCTCTCA GCCCCTGCTG GACATCTTGG AGGTGGTGGG AGGCTGGCCG 
GTGGCGATGG ACAGGTGGAA CGAGACCGTA GGACTCGAGT GGGAGCTGGA GCGGCAGCTG 
GCGCTGATGA ACTCACAGTT CAACAGGCGC GTCCTCATCG ACCTCTTCAT CTGGAACGAC 
GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAGC CCACCTTGGG CATGCCCTCC 
CGAGAGTACT ACTTCAACGG CGGCAGCAAC CGGAAGGTGC GGGAAGCCTA CCTGCAGTTC 
ATGGTGTCAG TGGCCACGTT GCTGCGGGAG GATGCAAACC TGCCCAGGGA CAGCTGCCTG 
GTGCAGGAGG ACATGGTGCA GGTGCTGGAG CTGGAGACAC AGCTGGCCAA GGCCACGGTA 
CCCCAGGAGG AGAGACACGA CGTCATCGCC TTGTACCACC GGATGGGACT GGAGGAGCTG 
CAAAGCCAGT TTGGCCTGAA GGGATTTAAC TGGACTCTGT TCATACAAAC TGTGCTATCC 
TCTGTCAAAA TCAAGCTGCT GCCAGATGAG GAAGTGGTGG TCTATGGCAT CCCCTACCTG 
CAGAACCTTG AAAACATCAT CGACACCTAC TCAGCCAGGA CCATACAGAA CTACCTGGTC 
TGGCGCCTGG TGCTGGACCG CATTGGTAGC CTAAGCCAGA GATTCAAGGA CACACGAGTG 
AACTACCGCA AGGCGCTGTT TGGCACAATG GTGGAGGAGG TGCGCTGGCG TGAATGTGTG 
GGCTACGTCA ACAGCAACAT GGAGAACGCC GTGGGCTCCC TCTACGTCAG GGAGGCGTTC 
CCTGGAGACA GCAAGAGCAT GGTCAGAGAA CTCATTGACA AGGTGCGGAC AGTGTTTGTG 
GAGACGCTGG ACGAGCTGGG CTGGATGGAC GAGGAGTCCA AGAAGAAGGC GCAGGAGAAG 
GCCATGAGCA TCCGGGAGCA GATCGGGCAC CCTGACTACA TCCTGGAGGA GATGAACAGG 
CGCCTGGACG AGGAGTACTC CAATCTGAAC TTCTCAGAGG ACCTGTACTT TGAGAACAGT 
CTGCAGAACC TCAAGGTGGG CGCCCAGCGG AGCCTCAGGA AGCTTCGGGA AAAGGTGGAC 
CCAAATCTCT GGATCATCGG GGCGGCGGTG GTCAATGCGT TCTACTCCCC AAACCGAAAC 
CAGATTGTAT TCCCTGCCGG GATCCTCCAG CCCCCCTTCT TCAGCAAGGA GCAGCCACAG 
GCCTTGAACT TTGGAGGCAT TGGGATGGTG ATCGGGCACG AGATCACGCA CGGCTTTGAC 
GACAATGGCC GGAACTTCGA CAAGAATGGC AACATGATGG ATTGGTGGAG TAACTTCTCC 
ACCCAGCACT TCCGGGAGCA GTCAGAGTGC ATGATCTACC AGTACGGCAA CTACTCCTGG 
GACCTGGCAG ACGAACAGAA CGTGAACGGA TTCAACACCC TTGGGGAAAA CATTGCTGAC 
AACGGAGGGG TGCGGCAAGC CTATAAGGTG TGGTGCGGGT CCTACCGGCC CGAGTTCGCC 
ATCCAATCCA TCAAGACAGA CGTCCACAGT CCCCTGAAGT ACAGGGTACT GGGGTCGCTG 
CAGAACCTGG CCGCCTTCGC AGACACGTTC CACTGTGCCC GGGGCACCCC CATGCACCCC 
AAGGAGCGAT GCCGCGTGTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMEL1 ( XM_017002314.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857803.1 Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu90513
Clone ID Related Accession (Same CDS sequence)	XM_017002314.1
Accession Version	XM_017002314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	membrane metallo-endopeptidase-like 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGT CCGAAGGCCC AGTGGGGATG GTGGAGAGCG CCGGCCGTGC AGGGCAGAAG 
CGCCCGGGGT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TGCTGGTGAC CGCTGCCCTG 
GTGGCCTTGG GTGTCCTCTA CGCCGACCGC AGAGGGAAGC AGCTGCCACG CCTTGCTAGC 
CGGCTGTGCT TCTTACAGGA GGAGAGGACC TTTGTAAAAC GAAAACCCCG AGGGATCCCA 
GAGGCCCAAG AGGTGAGCGA GGTCTGCACC ACCCCTGGCT GCGTGATAGC AGCTGCCAGG 
ATCCTCCAGA ACATGGACCC GACCACGGAA CCGTGTGACG ACTTCTACCA GTTTGCATGC 
GGAGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CAAGATACAG CATCTTTGAC 
GTCCTCCGCG ACGAGCTGGA GGTCATCCTC AAAGCGGTGC TGGAGAATTC GACTGCCAAG 
GACCGGCCGG CTGTGGAGAA GGCCAGGACG CTGTACCGCT CCTGCATGAA CCAGAGTGTG 
ATAGAGAAGC GAGGCTCTCA GCCCCTGCTG GACATCTTGG AGGTGGTGGG AGGCTGGCCG 
GTGGCGATGG ACAGGTGGAA CGAGACCGTA GGACTCGAGT GGGAGCTGGA GCGGCAGCTG 
GCGCTGATGA ACTCACAGTT CAACAGGCGC GTCCTCATCG ACCTCTTCAT CTGGAACGAC 
GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAGC CCACCTTGGG CATGCCCTCC 
CGAGAGTACT ACTTCAACGG CGGCAGCAAC CGGAAGGTGC GGGAAGCCTA CCTGCAGTTC 
ATGGTGTCAG TGGCCACGTT GCTGCGGGAG GATGCAAACC TGCCCAGGGA CAGCTGCCTG 
GTGCAGGAGG ACATGGTGCA GGTGCTGGAG CTGGAGACAC AGCTGGCCAA GGCCACGGTA 
CCCCAGGAGG AGAGACACGA CGTCATCGCC TTGTACCACC GGATGGGACT GGAGGAGCTG 
CAAAGCCAGT TTGGCCTGAA GGGATTTAAC TGGACTCTGT TCATACAAAC TGTGCTATCC 
TCTGTCAAAA TCAAGCTGCT GCCAGATGAG GAAGTGGTGG TCTATGGCAT CCCCTACCTG 
CAGAACCTTG AAAACATCAT CGACACCTAC TCAGCCAGGA CCATACAGAA CTACCTGGTC 
TGGCGCCTGG TGCTGGACCG CATTGGTAGC CTAAGCCAGA GATTCAAGGA CACACGAGTG 
AACTACCGCA AGGTCAGAGA ACTCATTGAC AAGGTGCGGA CAGTGTTTGT GGAGACGCTG 
GACGAGCTGG GCTGGATGGA CGAGGAGTCC AAGAAGAAGG CGCAGGAGAA GGCCATGAGC 
ATCCGGGAGC AGATCGGGCA CCCTGACTAC ATCCTGGAGG AGATGAACAG GCGCCTGGAC 
GAGGAGTACT CCAATCTGAA CTTCTCAGAG GACCTGTACT TTGAGAACAG TCTGCAGAAC 
CTCAAGGTGG GCGCCCAGCG GAGCCTCAGG AAGCTTCGGG AAAAGGTGGA CCCAAATCTC 
TGGATCATCG GGGCGGCGGT GGTCAATGCG TTCTACTCCC CAAACCGAAA CCAGATTGTA 
TTCCCTGCCG GGATCCTCCA GCCCCCCTTC TTCAGCAAGG AGCAGCCACA GGCCTTGAAC 
TTTGGAGGCA TTGGGATGGT GATCGGGCAC GAGATCACGC ACGGCTTTGA CGACAATGGC 
CGGAACTTCG ACAAGAATGG CAACATGATG GATTGGTGGA GTAACTTCTC CACCCAGCAC 
TTCCGGGAGC AGTCAGAGTG CATGATCTAC CAGTACGGCA ACTACTCCTG GGACCTGGCA 
GACGAACAGA ACGTGAACGG ATTCAACACC CTTGGGGAAA ACATTGCTGA CAACGGAGGG 
GTGCGGCAAG CCTATAAGGC CTACCTCAAG TGGATGGCAG AGGGTGGCAA GGACCAGCAG 
CTGCCCGGCC TGGATCTCAC CCATGAGCAG CTCTTCTTCA TCAACTATGC CCAGGTGTGG 
TGCGGGTCCT ACCGGCCCGA GTTCGCCATC CAATCCATCA AGACAGACGT CCACAGTCCC 
CTGAAGTACA GGGTACTGGG GTCGCTGCAG AACCTGGCCG CCTTCGCAGA CACGTTCCAC 
TGTGCCCGGG GCACCCCCAT GCACCCCAAG GAGCGATGCC GCGTGTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857803.1
CDS	215..2425
Translation	MGKSEGPVGMVESAGRAGQKRPGFLEGGLLLLLLLVTAALVALGVLYADRRGKQLPRLASRLCFLQEERTFVKRKPRGIPEAQEVSEVCTTPGCVIAAARILQNMDPTTEPCDDFYQFACGGWLRRHVIPETNSRYSIFDVLRDELEVILKAVLENSTAKDRPAVEKARTLYRSCMNQSVIEKRGSQPLLDILEVVGGWPVAMDRWNETVGLEWELERQLALMNSQFNRRVLIDLFIWNDDQNSSRHIIYIDQPTLGMPSREYYFNGGSNRKVREAYLQFMVSVATLLREDANLPRDSCLVQEDMVQVLELETQLAKATVPQEERHDVIALYHRMGLEELQSQFGLKGFNWTLFIQTVLSSVKIKLLPDEEVVVYGIPYLQNLENIIDTYSARTIQNYLVWRLVLDRIGSLSQRFKDTRVNYRKVRELIDKVRTVFVETLDELGWMDEESKKKAQEKAMSIREQIGHPDYILEEMNRRLDEEYSNLNFSEDLYFENSLQNLKVGAQRSLRKLREKVDPNLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKEQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMMDWWSNFSTQHFREQSECMIYQYGNYSWDLADEQNVNGFNTLGENIADNGGVRQAYKAYLKWMAEGGKDQQLPGLDLTHEQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLAAFADTFHCARGTPMHPKERCRVW

Target ORF information:

RefSeq Version	XM_017002314.1
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane metalloendopeptidase like 1 (MMEL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002314.1

ORF Insert Sequence:

ATGGGGAAGT CCGAAGGCCC AGTGGGGATG GTGGAGAGCG CCGGCCGTGC AGGGCAGAAG 
CGCCCGGGGT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TGCTGGTGAC CGCTGCCCTG 
GTGGCCTTGG GTGTCCTCTA CGCCGACCGC AGAGGGAAGC AGCTGCCACG CCTTGCTAGC 
CGGCTGTGCT TCTTACAGGA GGAGAGGACC TTTGTAAAAC GAAAACCCCG AGGGATCCCA 
GAGGCCCAAG AGGTGAGCGA GGTCTGCACC ACCCCTGGCT GCGTGATAGC AGCTGCCAGG 
ATCCTCCAGA ACATGGACCC GACCACGGAA CCGTGTGACG ACTTCTACCA GTTTGCATGC 
GGAGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CAAGATACAG CATCTTTGAC 
GTCCTCCGCG ACGAGCTGGA GGTCATCCTC AAAGCGGTGC TGGAGAATTC GACTGCCAAG 
GACCGGCCGG CTGTGGAGAA GGCCAGGACG CTGTACCGCT CCTGCATGAA CCAGAGTGTG 
ATAGAGAAGC GAGGCTCTCA GCCCCTGCTG GACATCTTGG AGGTGGTGGG AGGCTGGCCG 
GTGGCGATGG ACAGGTGGAA CGAGACCGTA GGACTCGAGT GGGAGCTGGA GCGGCAGCTG 
GCGCTGATGA ACTCACAGTT CAACAGGCGC GTCCTCATCG ACCTCTTCAT CTGGAACGAC 
GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAGC CCACCTTGGG CATGCCCTCC 
CGAGAGTACT ACTTCAACGG CGGCAGCAAC CGGAAGGTGC GGGAAGCCTA CCTGCAGTTC 
ATGGTGTCAG TGGCCACGTT GCTGCGGGAG GATGCAAACC TGCCCAGGGA CAGCTGCCTG 
GTGCAGGAGG ACATGGTGCA GGTGCTGGAG CTGGAGACAC AGCTGGCCAA GGCCACGGTA 
CCCCAGGAGG AGAGACACGA CGTCATCGCC TTGTACCACC GGATGGGACT GGAGGAGCTG 
CAAAGCCAGT TTGGCCTGAA GGGATTTAAC TGGACTCTGT TCATACAAAC TGTGCTATCC 
TCTGTCAAAA TCAAGCTGCT GCCAGATGAG GAAGTGGTGG TCTATGGCAT CCCCTACCTG 
CAGAACCTTG AAAACATCAT CGACACCTAC TCAGCCAGGA CCATACAGAA CTACCTGGTC 
TGGCGCCTGG TGCTGGACCG CATTGGTAGC CTAAGCCAGA GATTCAAGGA CACACGAGTG 
AACTACCGCA AGGTCAGAGA ACTCATTGAC AAGGTGCGGA CAGTGTTTGT GGAGACGCTG 
GACGAGCTGG GCTGGATGGA CGAGGAGTCC AAGAAGAAGG CGCAGGAGAA GGCCATGAGC 
ATCCGGGAGC AGATCGGGCA CCCTGACTAC ATCCTGGAGG AGATGAACAG GCGCCTGGAC 
GAGGAGTACT CCAATCTGAA CTTCTCAGAG GACCTGTACT TTGAGAACAG TCTGCAGAAC 
CTCAAGGTGG GCGCCCAGCG GAGCCTCAGG AAGCTTCGGG AAAAGGTGGA CCCAAATCTC 
TGGATCATCG GGGCGGCGGT GGTCAATGCG TTCTACTCCC CAAACCGAAA CCAGATTGTA 
TTCCCTGCCG GGATCCTCCA GCCCCCCTTC TTCAGCAAGG AGCAGCCACA GGCCTTGAAC 
TTTGGAGGCA TTGGGATGGT GATCGGGCAC GAGATCACGC ACGGCTTTGA CGACAATGGC 
CGGAACTTCG ACAAGAATGG CAACATGATG GATTGGTGGA GTAACTTCTC CACCCAGCAC 
TTCCGGGAGC AGTCAGAGTG CATGATCTAC CAGTACGGCA ACTACTCCTG GGACCTGGCA 
GACGAACAGA ACGTGAACGG ATTCAACACC CTTGGGGAAA ACATTGCTGA CAACGGAGGG 
GTGCGGCAAG CCTATAAGGC CTACCTCAAG TGGATGGCAG AGGGTGGCAA GGACCAGCAG 
CTGCCCGGCC TGGATCTCAC CCATGAGCAG CTCTTCTTCA TCAACTATGC CCAGGTGTGG 
TGCGGGTCCT ACCGGCCCGA GTTCGCCATC CAATCCATCA AGACAGACGT CCACAGTCCC 
CTGAAGTACA GGGTACTGGG GTCGCTGCAG AACCTGGCCG CCTTCGCAGA CACGTTCCAC 
TGTGCCCGGG GCACCCCCAT GCACCCCAAG GAGCGATGCC GCGTGTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

In silico based investigation of dynamic variations in neprilysin (NEP and NEP2) proteins for extracting the point of specificity.
Molecular bioSystems12(3)1024-36(2016 Mar)
Ul-Haq Z,Usmani S,Iqbal S,Zia SR

Replication of GWAS Coding SNPs Implicates MMEL1 as a Potential Susceptibility Locus among Saudi Arabian Celiac Disease Patients.
Disease markers2015351673(2015)
Saadah OI,Shaik NA,Banaganapalli B,Salama MA,Al-Harthi SE,Wang J,Shawoosh HA,Alghamdi SA,Bin-Taleb YY,Alhussaini BH,Elango R,Al-Aama JY

Novel rheumatoid arthritis susceptibility locus at 22q12 identified in an extended UK genome-wide association study.
Arthritis & rheumatology (Hoboken, N.J.)66(1)24-30(2014 Jan)
Orozco G,Viatte S,Bowes J,Martin P,Wilson AG,Morgan AW,Steer S,Wordsworth P,Hocking LJ,,,,Barton A,Worthington J,Eyre S

Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease.
Nature491(7422)119-24(2012 Nov)
Jostins L,Ripke S,Weersma RK,Duerr RH,McGovern DP,Hui KY,Lee JC,Schumm LP,Sharma Y,Anderson CA,Essers J,Mitrovic M,Ning K,Cleynen I,Theatre E,Spain SL,Raychaudhuri S,Goyette P,Wei Z,Abraham C,Achkar JP,Ahmad T,Amininejad L,Ananthakrishnan AN,Andersen V,Andrews JM,Baidoo L,Balschun T,Bampton PA,Bitton A,Boucher G,Brand S,B?ning C,Cohain A,Cichon S,D'Amato M,De Jong D,Devaney KL,Dubinsky M,Edwards C,Ellinghaus D,Ferguson LR,Franchimont D,Fransen K,Gearry R,Georges M,Gieger C,Glas J,Haritunians T,Hart A,Hawkey C,Hedl M,Hu X,Karlsen TH,Kupcinskas L,Kugathasan S,Latiano A,Laukens D,Lawrance IC,Lees CW,Louis E,Mahy G,Mansfield J,Morgan AR,Mowat C,Newman W,Palmieri O,Ponsioen CY,Potocnik U,Prescott NJ,Regueiro M,Rotter JI,Russell RK,Sanderson JD,Sans M,Satsangi J,Schreiber S,Simms LA,Sventoraityte J,Targan SR,Taylor KD,Tremelling M,Verspaget HW,De Vos M,Wijmenga C,Wilson DC,Winkelmann J,Xavier RJ,Zeissig S,Zhang B,Zhang CK,Zhao H,,Silverberg MS,Annese V,Hakonarson H,Brant SR,Radford-Smith G,Mathew CG,Rioux JD,Schadt EE,Daly MJ,Franke A,Parkes M,Vermeire S,Barrett JC,Cho JH

A genome-wide association study in Chinese men identifies three risk loci for non-obstructive azoospermia.
Nature genetics44(2)183-6(2011 Dec)
Hu Z,Xia Y,Guo X,Dai J,Li H,Hu H,Jiang Y,Lu F,Wu Y,Yang X,Li H,Yao B,Lu C,Xiong C,Li Z,Gui Y,Liu J,Zhou Z,Shen H,Wang X,Sha J

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Gene Symbol	MMEL1
Entrez Gene ID	79258
Full Name	membrane metalloendopeptidase like 1
Synonyms	MMEL2,NEP2,NEPII,NL1,NL2,SEP
General protein information	Preferred Names membrane metalloendopeptidase like 1 Names membrane metallo-endopeptidase-like 1 NEP2(m) membrane metallo-endopeptidase-like 2 neprilysin II neprilysin-2 soluble secreted endopeptidase zinc metallopeptidase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.32
Summary	The protein encoded by this gene is a member of the neutral endopeptidase (NEP) or membrane metallo-endopeptidase (MME) family. Family members play important roles in pain perception, arterial pressure regulation, phosphate metabolism and homeostasis. This protein is a type II transmembrane protein and is thought to be expressed as a secreted protein. This gene is expressed mainly in testis with weak expression in the brain, kidney, and heart. [provided by RefSeq, Jul 2008].

Gallus gallus (chicken)	MMEL1	XP_001233077.3
Danio rerio (zebrafish)	mmel1	XP_689191.3
Canis lupus familiaris (dog)	MMEL1	XP_005620485.1
Mus musculus (house mouse)	Mmel1	NP_038811.2
Xenopus tropicalis (tropical clawed frog)	mmel1	NP_001120567.1
Homo sapiens (human)	MMEL1	NP_258428.2
Drosophila melanogaster (fruit fly)	Nep4	NP_650904.3
Pan troglodytes (chimpanzee)	MMEL1	XP_001150860.1
Bos taurus (cattle)	MMEL1	NP_001180065.1
Rattus norvegicus (Norway rat)	Mmel1	NP_001101467.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003106	XP_312790.4
Caenorhabditis elegans (roundworm)	nep-2	NP_494343.2

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MMEL1 cDNA ORF clone, Homo sapiens(human)

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