MMEL1 cDNA ORF clone, Canis lupus familiaris(dog)

The following MMEL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMEL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe06775 XM_005620429.2
Latest version!
Canis lupus familiaris membrane metallo-endopeptidase-like 1 (MMEL1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCe06776 XM_022418947.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCe288363 XM_022418932.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OCe288363 XM_022418933.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OCe288364 XM_022418934.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OCe288365 XM_022418935.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OCe288366 XM_022418936.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OCe288367 XM_022418937.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OCe288368 XM_022418938.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OCe288369 XM_022418939.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OCe288370 XM_022418940.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OCe288371 XM_022418941.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCe288372 XM_022418943.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCe288373 XM_022418945.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCe288373 XM_022418944.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCe288374 XM_022418946.1
Latest version!
Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe06775
    Clone ID Related Accession (Same CDS sequence) XM_005620429.2
    Accession Version XM_005620429.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product membrane metallo-endopeptidase-like 1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGGGG TGCTAGAGAA TGCCACCACC
    CAGGACAGGC CGGCTGTGCA GAAGGCCAAG ATGCTATACC GCTCCTGCAT GAACCAGAGC
    GTGATAGAGA AACGAGACTC ACAGCCCCTG CTAAACATCC TGGACGTGAT GGGAGGCTGG
    CCAGTGACCA TGGATAAGTG GAACCAGAGC GTAGCCCGAG GCACTGACTC CCCAGGCCCC
    AAGTGGGAGC TGGAGCAGCA GCTGGCCTTG ATGAACACAC AGTTCAACAG GCGTGTCCTC
    ATTGACCTCT TCATCTGGAA CGATGACCAG AATTCTAGCC GGCATATCAT CTACATAGAC
    CAGCCCACCT TGGGCATGCC ATCCAGAGAG TACTATTTCA ACGAGGGCAG CAACCGGAAG
    GTGCGGGAAG CCTACCTGCA GTTCATGATG TCGGTGGCCA TGATGCTGCG GGCAGACATG
    AACCTCCCAG AGAACAGCTA CCTGGTGCGG GAGGACATGG TGCAGGTGCT GGAGCTGGAG
    ACGCAGCTGG CAAACGCCAC GGCCCCCCAG GAGGAGAGGC ACGATGTCAC TGCCCTATAT
    CATAGGATGG GCCTGAAGGA ACTCCAGAGC AAGTTTAGCC TGAAGGGGTT TAACTGGACC
    CTCTTCATAC AATCTGTGCT GTCCTCTGTC AAAATCAAAC TGTTGCCAGA TGAAGAAGTG
    GTGGTCTATG GCATCCCCTA CCTTCAGAAC CTTGAAGAAA TCATCGATGT CTACTCAGCC
    AGGACCATGC AGAACTACCT GGTCTGGCGC CTGGTGCTGG ACCGCATTAG TAGCCTGAGC
    CAGCGATTCA AGGATGCACG GGCAAACTAC CGCAAGGCGC TGTACGGCAC AACCGTGGAA
    GAGGTGCGCT GGCGAGAGTG TGTTAGCTAC GTCAACAGCA ACATGGAGAG CGCTGTGGGC
    TCCCTCTACG TCAGGGAGGC CTTCCCTGGA GACAGCAAGG ACGCGGTCAG AGAGCTCATC
    GACAAGGTAC GGGCGGTATT CGTGGAGACC CTGGATGAGC TGGGCTGGAT GGACGAGGAG
    TCCAAGAAGA AGGCCCAGGA GAAGGCCATG AACATCCGCG AGCAGATTGG GTACCCAGAC
    TACATTCTGG AGGAAAGAAA CAAGCACCTG GACGAGGAGT ACTCCAATCT GAACTTCTCG
    GAGGACCAGT ACTTTGAAAA CGGTCTCCAG AACCTCAAGG CTGGCGCCCA GCGGAGCCTC
    AAGAAGCTGC GGGAGAAAGT AGACCAGAAC CTCTGGATCA TTGGGGCGGC CGTGGTTAAT
    GCATTCTACT CCCCGAACCG AAACCAAATA GTGTTCCCTG CTGGGATCCT ACAGCCTCCA
    TTCTTCAGCA AACATCAGCC TCAGGCCTTG AACTTCGGGG GCATTGGGAT GGTGATTGGG
    CATGAGATCA CCCACGGCTT TGACGACAAC GGCCGTAACT TTGACAAGAA TGGGAACATG
    CTGGATTGGT GGAGCAATTT CTCAGCCCAG CACTTCCGGG AGCAGTCGGA GTGCATGGTC
    CACCAGTACG GGAACTACTC CTGGGACCTG GCGGACAACC AAAACGTGAA CGGGTTCAGC
    ACACTCGGGG AAAACATCGC CGACAACGGA GGGGTGCGGC AGGCATACAA GGCTTACCTC
    AAGTGGATGG CAGAAGGCGG CAAGGACCAG CAGCTGCCAG GGCTGGGACT GACCTACAAC
    CAGCTGTTCT TTATCAACTA TGCCCAGGTG TGGTGTGGGT CCTACCGGCC TGAGTTTGCC
    ATACAGTCCA TCAAGACCGA CGTCCATAGC CCCCTGAAGT ACAGGGTGCT GGGCTCGCTG
    CAGAACCTGG CAGCCTTCTC GGAGGCCTTC CACTGTGCCC AGGGCAGCCC GATGCACCCG
    CACCAGCGAT GCCGCGTCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620486.1
    CDS794..3157
    Translation

    Target ORF information:

    RefSeq Version XM_005620429.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris membrane metallo-endopeptidase-like 1 (MMEL1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620429.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGGGG TGCTAGAGAA TGCCACCACC
    CAGGACAGGC CGGCTGTGCA GAAGGCCAAG ATGCTATACC GCTCCTGCAT GAACCAGAGC
    GTGATAGAGA AACGAGACTC ACAGCCCCTG CTAAACATCC TGGACGTGAT GGGAGGCTGG
    CCAGTGACCA TGGATAAGTG GAACCAGAGC GTAGCCCGAG GCACTGACTC CCCAGGCCCC
    AAGTGGGAGC TGGAGCAGCA GCTGGCCTTG ATGAACACAC AGTTCAACAG GCGTGTCCTC
    ATTGACCTCT TCATCTGGAA CGATGACCAG AATTCTAGCC GGCATATCAT CTACATAGAC
    CAGCCCACCT TGGGCATGCC ATCCAGAGAG TACTATTTCA ACGAGGGCAG CAACCGGAAG
    GTGCGGGAAG CCTACCTGCA GTTCATGATG TCGGTGGCCA TGATGCTGCG GGCAGACATG
    AACCTCCCAG AGAACAGCTA CCTGGTGCGG GAGGACATGG TGCAGGTGCT GGAGCTGGAG
    ACGCAGCTGG CAAACGCCAC GGCCCCCCAG GAGGAGAGGC ACGATGTCAC TGCCCTATAT
    CATAGGATGG GCCTGAAGGA ACTCCAGAGC AAGTTTAGCC TGAAGGGGTT TAACTGGACC
    CTCTTCATAC AATCTGTGCT GTCCTCTGTC AAAATCAAAC TGTTGCCAGA TGAAGAAGTG
    GTGGTCTATG GCATCCCCTA CCTTCAGAAC CTTGAAGAAA TCATCGATGT CTACTCAGCC
    AGGACCATGC AGAACTACCT GGTCTGGCGC CTGGTGCTGG ACCGCATTAG TAGCCTGAGC
    CAGCGATTCA AGGATGCACG GGCAAACTAC CGCAAGGCGC TGTACGGCAC AACCGTGGAA
    GAGGTGCGCT GGCGAGAGTG TGTTAGCTAC GTCAACAGCA ACATGGAGAG CGCTGTGGGC
    TCCCTCTACG TCAGGGAGGC CTTCCCTGGA GACAGCAAGG ACGCGGTCAG AGAGCTCATC
    GACAAGGTAC GGGCGGTATT CGTGGAGACC CTGGATGAGC TGGGCTGGAT GGACGAGGAG
    TCCAAGAAGA AGGCCCAGGA GAAGGCCATG AACATCCGCG AGCAGATTGG GTACCCAGAC
    TACATTCTGG AGGAAAGAAA CAAGCACCTG GACGAGGAGT ACTCCAATCT GAACTTCTCG
    GAGGACCAGT ACTTTGAAAA CGGTCTCCAG AACCTCAAGG CTGGCGCCCA GCGGAGCCTC
    AAGAAGCTGC GGGAGAAAGT AGACCAGAAC CTCTGGATCA TTGGGGCGGC CGTGGTTAAT
    GCATTCTACT CCCCGAACCG AAACCAAATA GTGTTCCCTG CTGGGATCCT ACAGCCTCCA
    TTCTTCAGCA AACATCAGCC TCAGGCCTTG AACTTCGGGG GCATTGGGAT GGTGATTGGG
    CATGAGATCA CCCACGGCTT TGACGACAAC GGCCGTAACT TTGACAAGAA TGGGAACATG
    CTGGATTGGT GGAGCAATTT CTCAGCCCAG CACTTCCGGG AGCAGTCGGA GTGCATGGTC
    CACCAGTACG GGAACTACTC CTGGGACCTG GCGGACAACC AAAACGTGAA CGGGTTCAGC
    ACACTCGGGG AAAACATCGC CGACAACGGA GGGGTGCGGC AGGCATACAA GGCTTACCTC
    AAGTGGATGG CAGAAGGCGG CAAGGACCAG CAGCTGCCAG GGCTGGGACT GACCTACAAC
    CAGCTGTTCT TTATCAACTA TGCCCAGGTG TGGTGTGGGT CCTACCGGCC TGAGTTTGCC
    ATACAGTCCA TCAAGACCGA CGTCCATAGC CCCCTGAAGT ACAGGGTGCT GGGCTCGCTG
    CAGAACCTGG CAGCCTTCTC GGAGGCCTTC CACTGTGCCC AGGGCAGCCC GATGCACCCG
    CACCAGCGAT GCCGCGTCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06776
    Clone ID Related Accession (Same CDS sequence) XM_022418947.1
    Accession Version XM_022418947.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGGGG TGCTAGAGAA TGCCACCACC
    CAGGACAGGC CGGCTGTGCA GAAGGCCAAG ATGCTATACC GCTCCTGCAT GAACCAGAGC
    GTGATAGAGA AACGAGACTC ACAGCCCCTG CTAAACATCC TGGACGTGAT GGGAGGCTGG
    CCAGTGACCA TGGATAAGTG GAACCAGAGC GTAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTCAGA GAGCTCATCG ACAAGGTACG GGCGGTATTC
    GTGGAGACCC TGGATGAGCT GGGCTGGATG GACGAGGAGT CCAAGAAGAA GGCCCAGGAG
    AAGGCCATGA ACATCCGCGA GCAGATTGGG TACCCAGACT ACATTCTGGA GGAAAGAAAC
    AAGCACCTGG ACGAGGAGTA CTCCAATCTG AACTTCTCGG AGGACCAGTA CTTTGAAAAC
    GGTCTCCAGA ACCTCAAGGC TGGCGCCCAG CGGAGCCTCA AGAAGCTGCG GGAGAAAGTA
    GACCAGAACC TCTGGATCAT TGGGGCGGCC GTGGTTAATG CATTCTACTC CCCGAACCGA
    AACCAAATAG TGTTCCCTGC TGGGATCCTA CAGCCTCCAT TCTTCAGCAA ACATCAGCCT
    CAGGCCTTGA ACTTCGGGGG CATTGGGATG GTGATTGGGC ATGAGATCAC CCACGGCTTT
    GACGACAACG GCCGTAACTT TGACAAGAAT GGGAACATGC TGGATTGGTG GAGCAATTTC
    TCAGCCCAGC ACTTCCGGGA GCAGTCGGAG TGCATGGTCC ACCAGTACGG GAACTACTCC
    TGGGACCTGG CGGACAACCA AAACGTGAAC GGGTTCAGCA CACTCGGGGA AAACATCGCC
    GACAACGGAG GGGTGCGGCA GGCATACAAG GCTTACCTCA AGTGGATGGC AGAAGGCGGC
    AAGGACCAGC AGCTGCCAGG GCTGGGACTG ACCTACAACC AGCTGTTCTT TATCAACTAT
    GCCCAGGTGT GGTGTGGGTC CTACCGGCCT GAGTTTGCCA TACAGTCCAT CAAGACCGAC
    GTCCATAGCC CCCTGAAGTA CAGGGTGCTG GGCTCGCTGC AGAACCTGGC AGCCTTCTCG
    GAGGCCTTCC ACTGTGCCCA GGGCAGCCCG ATGCACCCGC ACCAGCGATG CCGCGTCTGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274655.1
    CDS206..2548
    Translation

    Target ORF information:

    RefSeq Version XM_022418947.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418947.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGGGG TGCTAGAGAA TGCCACCACC
    CAGGACAGGC CGGCTGTGCA GAAGGCCAAG ATGCTATACC GCTCCTGCAT GAACCAGAGC
    GTGATAGAGA AACGAGACTC ACAGCCCCTG CTAAACATCC TGGACGTGAT GGGAGGCTGG
    CCAGTGACCA TGGATAAGTG GAACCAGAGC GTAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTCAGA GAGCTCATCG ACAAGGTACG GGCGGTATTC
    GTGGAGACCC TGGATGAGCT GGGCTGGATG GACGAGGAGT CCAAGAAGAA GGCCCAGGAG
    AAGGCCATGA ACATCCGCGA GCAGATTGGG TACCCAGACT ACATTCTGGA GGAAAGAAAC
    AAGCACCTGG ACGAGGAGTA CTCCAATCTG AACTTCTCGG AGGACCAGTA CTTTGAAAAC
    GGTCTCCAGA ACCTCAAGGC TGGCGCCCAG CGGAGCCTCA AGAAGCTGCG GGAGAAAGTA
    GACCAGAACC TCTGGATCAT TGGGGCGGCC GTGGTTAATG CATTCTACTC CCCGAACCGA
    AACCAAATAG TGTTCCCTGC TGGGATCCTA CAGCCTCCAT TCTTCAGCAA ACATCAGCCT
    CAGGCCTTGA ACTTCGGGGG CATTGGGATG GTGATTGGGC ATGAGATCAC CCACGGCTTT
    GACGACAACG GCCGTAACTT TGACAAGAAT GGGAACATGC TGGATTGGTG GAGCAATTTC
    TCAGCCCAGC ACTTCCGGGA GCAGTCGGAG TGCATGGTCC ACCAGTACGG GAACTACTCC
    TGGGACCTGG CGGACAACCA AAACGTGAAC GGGTTCAGCA CACTCGGGGA AAACATCGCC
    GACAACGGAG GGGTGCGGCA GGCATACAAG GCTTACCTCA AGTGGATGGC AGAAGGCGGC
    AAGGACCAGC AGCTGCCAGG GCTGGGACTG ACCTACAACC AGCTGTTCTT TATCAACTAT
    GCCCAGGTGT GGTGTGGGTC CTACCGGCCT GAGTTTGCCA TACAGTCCAT CAAGACCGAC
    GTCCATAGCC CCCTGAAGTA CAGGGTGCTG GGCTCGCTGC AGAACCTGGC AGCCTTCTCG
    GAGGCCTTCC ACTGTGCCCA GGGCAGCCCG ATGCACCCGC ACCAGCGATG CCGCGTCTGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288363
    Clone ID Related Accession (Same CDS sequence) XM_022418932.1 , XM_022418933.1
    Accession Version XM_022418932.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTGGGT GCTCGGCACT GGGACTGCTC CGCACCCCTG
    CCCATGGTCC ATTGCCCAGG GCCAGCAGGG ACGGGGCTTT GGACCAGACC CCTGCAGCGA
    CCCCGGAGCT GGTCATGCAG GACAAGGGCC ATGCACTCCC CCCAGGTCAG AGAGCTCATC
    GACAAGGTAC GGGCGGTATT CGTGGAGACC CTGGATGAGC TGGGCTGGAT GGACGAGGAG
    TCCAAGAAGA AGGCCCAGGA GAAGGCCATG AACATCCGCG AGCAGATTGG GTACCCAGAC
    TACATTCTGG AGGAAAGAAA CAAGCACCTG GACGAGGAGT ACTCCAATCT GAACTTCTCG
    GAGGACCAGT ACTTTGAAAA CGGTCTCCAG AACCTCAAGG CTGGCGCCCA GCGGAGCCTC
    AAGAAGCTGC GGGAGAAAGT AGACCAGAAC CTGGGCCCCA AGCAGGGGAG CCGCATTCAG
    CCAGCAGGGG GCCAGGACGG AGGACTCCCA GGCACGGGCT TGGGTGACCT GCCCGTTCAT
    CACAGCTGGA TCATTGGGGC GGCCGTGGTT AATGCATTCT ACTCCCCGAA CCGAAACCAA
    ATAGTGTTCC CTGCTGGGAT CCTACAGCCT CCATTCTTCA GCAAACATCA GCCTCAGGCC
    TTGAACTTCG GGGGCATTGG GATGGTGATT GGGCATGAGA TCACCCACGG CTTTGACGAC
    AACGGCCGTA ACTTTGACAA GAATGGGAAC ATGCTGGATT GGTGGAGCAA TTTCTCAGCC
    CAGCACTTCC GGGAGCAGTC GGAGTGCATG GTCCACCAGT ACGGGAACTA CTCCTGGGAC
    CTGGCGGACA ACCAAAACGT GAACGGGTTC AGCACACTCG GGGAAAACAT CGCCGACAAC
    GGAGGGGTGC GGCAGGCATA CAAGGCTTAC CTCAAGTGGA TGGCAGAAGG CGGCAAGGAC
    CAGCAGCTGC CAGGGCTGGG ACTGACCTAC AACCAGCTGT TCTTTATCAA CTATGCCCAG
    GTGTGGTGTG GGTCCTACCG GCCTGAGTTT GCCATACAGT CCATCAAGAC CGACGTCCAT
    AGCCCCCTGA AGTACAGGGT GCTGGGCTCG CTGCAGAACC TGGCAGCCTT CTCGGAGGCC
    TTCCACTGTG CCCAGGGCAG CCCGATGCAC CCGCACCAGC GATGCCGCGT CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274640.1
    CDS222..2858
    Translation

    Target ORF information:

    RefSeq Version XM_022418932.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418932.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTGGGT GCTCGGCACT GGGACTGCTC CGCACCCCTG
    CCCATGGTCC ATTGCCCAGG GCCAGCAGGG ACGGGGCTTT GGACCAGACC CCTGCAGCGA
    CCCCGGAGCT GGTCATGCAG GACAAGGGCC ATGCACTCCC CCCAGGTCAG AGAGCTCATC
    GACAAGGTAC GGGCGGTATT CGTGGAGACC CTGGATGAGC TGGGCTGGAT GGACGAGGAG
    TCCAAGAAGA AGGCCCAGGA GAAGGCCATG AACATCCGCG AGCAGATTGG GTACCCAGAC
    TACATTCTGG AGGAAAGAAA CAAGCACCTG GACGAGGAGT ACTCCAATCT GAACTTCTCG
    GAGGACCAGT ACTTTGAAAA CGGTCTCCAG AACCTCAAGG CTGGCGCCCA GCGGAGCCTC
    AAGAAGCTGC GGGAGAAAGT AGACCAGAAC CTGGGCCCCA AGCAGGGGAG CCGCATTCAG
    CCAGCAGGGG GCCAGGACGG AGGACTCCCA GGCACGGGCT TGGGTGACCT GCCCGTTCAT
    CACAGCTGGA TCATTGGGGC GGCCGTGGTT AATGCATTCT ACTCCCCGAA CCGAAACCAA
    ATAGTGTTCC CTGCTGGGAT CCTACAGCCT CCATTCTTCA GCAAACATCA GCCTCAGGCC
    TTGAACTTCG GGGGCATTGG GATGGTGATT GGGCATGAGA TCACCCACGG CTTTGACGAC
    AACGGCCGTA ACTTTGACAA GAATGGGAAC ATGCTGGATT GGTGGAGCAA TTTCTCAGCC
    CAGCACTTCC GGGAGCAGTC GGAGTGCATG GTCCACCAGT ACGGGAACTA CTCCTGGGAC
    CTGGCGGACA ACCAAAACGT GAACGGGTTC AGCACACTCG GGGAAAACAT CGCCGACAAC
    GGAGGGGTGC GGCAGGCATA CAAGGCTTAC CTCAAGTGGA TGGCAGAAGG CGGCAAGGAC
    CAGCAGCTGC CAGGGCTGGG ACTGACCTAC AACCAGCTGT TCTTTATCAA CTATGCCCAG
    GTGTGGTGTG GGTCCTACCG GCCTGAGTTT GCCATACAGT CCATCAAGAC CGACGTCCAT
    AGCCCCCTGA AGTACAGGGT GCTGGGCTCG CTGCAGAACC TGGCAGCCTT CTCGGAGGCC
    TTCCACTGTG CCCAGGGCAG CCCGATGCAC CCGCACCAGC GATGCCGCGT CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288363
    Clone ID Related Accession (Same CDS sequence) XM_022418932.1 , XM_022418933.1
    Accession Version XM_022418933.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTGGGT GCTCGGCACT GGGACTGCTC CGCACCCCTG
    CCCATGGTCC ATTGCCCAGG GCCAGCAGGG ACGGGGCTTT GGACCAGACC CCTGCAGCGA
    CCCCGGAGCT GGTCATGCAG GACAAGGGCC ATGCACTCCC CCCAGGTCAG AGAGCTCATC
    GACAAGGTAC GGGCGGTATT CGTGGAGACC CTGGATGAGC TGGGCTGGAT GGACGAGGAG
    TCCAAGAAGA AGGCCCAGGA GAAGGCCATG AACATCCGCG AGCAGATTGG GTACCCAGAC
    TACATTCTGG AGGAAAGAAA CAAGCACCTG GACGAGGAGT ACTCCAATCT GAACTTCTCG
    GAGGACCAGT ACTTTGAAAA CGGTCTCCAG AACCTCAAGG CTGGCGCCCA GCGGAGCCTC
    AAGAAGCTGC GGGAGAAAGT AGACCAGAAC CTGGGCCCCA AGCAGGGGAG CCGCATTCAG
    CCAGCAGGGG GCCAGGACGG AGGACTCCCA GGCACGGGCT TGGGTGACCT GCCCGTTCAT
    CACAGCTGGA TCATTGGGGC GGCCGTGGTT AATGCATTCT ACTCCCCGAA CCGAAACCAA
    ATAGTGTTCC CTGCTGGGAT CCTACAGCCT CCATTCTTCA GCAAACATCA GCCTCAGGCC
    TTGAACTTCG GGGGCATTGG GATGGTGATT GGGCATGAGA TCACCCACGG CTTTGACGAC
    AACGGCCGTA ACTTTGACAA GAATGGGAAC ATGCTGGATT GGTGGAGCAA TTTCTCAGCC
    CAGCACTTCC GGGAGCAGTC GGAGTGCATG GTCCACCAGT ACGGGAACTA CTCCTGGGAC
    CTGGCGGACA ACCAAAACGT GAACGGGTTC AGCACACTCG GGGAAAACAT CGCCGACAAC
    GGAGGGGTGC GGCAGGCATA CAAGGCTTAC CTCAAGTGGA TGGCAGAAGG CGGCAAGGAC
    CAGCAGCTGC CAGGGCTGGG ACTGACCTAC AACCAGCTGT TCTTTATCAA CTATGCCCAG
    GTGTGGTGTG GGTCCTACCG GCCTGAGTTT GCCATACAGT CCATCAAGAC CGACGTCCAT
    AGCCCCCTGA AGTACAGGGT GCTGGGCTCG CTGCAGAACC TGGCAGCCTT CTCGGAGGCC
    TTCCACTGTG CCCAGGGCAG CCCGATGCAC CCGCACCAGC GATGCCGCGT CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274641.1
    CDS246..2882
    Translation

    Target ORF information:

    RefSeq Version XM_022418933.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418933.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTGGGT GCTCGGCACT GGGACTGCTC CGCACCCCTG
    CCCATGGTCC ATTGCCCAGG GCCAGCAGGG ACGGGGCTTT GGACCAGACC CCTGCAGCGA
    CCCCGGAGCT GGTCATGCAG GACAAGGGCC ATGCACTCCC CCCAGGTCAG AGAGCTCATC
    GACAAGGTAC GGGCGGTATT CGTGGAGACC CTGGATGAGC TGGGCTGGAT GGACGAGGAG
    TCCAAGAAGA AGGCCCAGGA GAAGGCCATG AACATCCGCG AGCAGATTGG GTACCCAGAC
    TACATTCTGG AGGAAAGAAA CAAGCACCTG GACGAGGAGT ACTCCAATCT GAACTTCTCG
    GAGGACCAGT ACTTTGAAAA CGGTCTCCAG AACCTCAAGG CTGGCGCCCA GCGGAGCCTC
    AAGAAGCTGC GGGAGAAAGT AGACCAGAAC CTGGGCCCCA AGCAGGGGAG CCGCATTCAG
    CCAGCAGGGG GCCAGGACGG AGGACTCCCA GGCACGGGCT TGGGTGACCT GCCCGTTCAT
    CACAGCTGGA TCATTGGGGC GGCCGTGGTT AATGCATTCT ACTCCCCGAA CCGAAACCAA
    ATAGTGTTCC CTGCTGGGAT CCTACAGCCT CCATTCTTCA GCAAACATCA GCCTCAGGCC
    TTGAACTTCG GGGGCATTGG GATGGTGATT GGGCATGAGA TCACCCACGG CTTTGACGAC
    AACGGCCGTA ACTTTGACAA GAATGGGAAC ATGCTGGATT GGTGGAGCAA TTTCTCAGCC
    CAGCACTTCC GGGAGCAGTC GGAGTGCATG GTCCACCAGT ACGGGAACTA CTCCTGGGAC
    CTGGCGGACA ACCAAAACGT GAACGGGTTC AGCACACTCG GGGAAAACAT CGCCGACAAC
    GGAGGGGTGC GGCAGGCATA CAAGGCTTAC CTCAAGTGGA TGGCAGAAGG CGGCAAGGAC
    CAGCAGCTGC CAGGGCTGGG ACTGACCTAC AACCAGCTGT TCTTTATCAA CTATGCCCAG
    GTGTGGTGTG GGTCCTACCG GCCTGAGTTT GCCATACAGT CCATCAAGAC CGACGTCCAT
    AGCCCCCTGA AGTACAGGGT GCTGGGCTCG CTGCAGAACC TGGCAGCCTT CTCGGAGGCC
    TTCCACTGTG CCCAGGGCAG CCCGATGCAC CCGCACCAGC GATGCCGCGT CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288364
    Clone ID Related Accession (Same CDS sequence) XM_022418934.1
    Accession Version XM_022418934.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2616bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGGCCCCAA GTGGGAGCTG GAGCAGCAGC TGGCCTTGAT GAACACACAG
    TTCAACAGGC GTGTCCTCAT TGACCTCTTC ATCTGGAACG ATGACCAGAA TTCTAGCCGG
    CATATCATCT ACATAGACCA GCCCACCTTG GGCATGCCAT CCAGAGAGTA CTATTTCAAC
    GAGGGCAGCA ACCGGAAGGT GCGGGAAGCC TACCTGCAGT TCATGATGTC GGTGGCCATG
    ATGCTGCGGG CAGACATGAA CCTCCCAGAG AACAGCTACC TGGTGCGGGA GGACATGGTG
    CAGGTGCTGG AGCTGGAGAC GCAGCTGGCA AACGCCACGG CCCCCCAGGA GGAGAGGCAC
    GATGTCACTG CCCTATATCA TAGGATGGGC CTGAAGGAAC TCCAGAGCAA GTTTAGCCTG
    AAGGGGTTTA ACTGGACCCT CTTCATACAA TCTGTGCTGT CCTCTGTCAA AATCAAACTG
    TTGCCAGATG AAGAAGTGGT GGTCTATGGC ATCCCCTACC TTCAGAACCT TGAAGAAATC
    ATCGATGTCT ACTCAGCCAG GACCATGCAG AACTACCTGG TCTGGCGCCT GGTGCTGGAC
    CGCATTAGTA GCCTGAGCCA GCGATTCAAG GATGCACGGG CAAACTACCG CAAGGCGCTG
    TACGGCACAA CCGTGGAAGA GGTGCGCTGG CGAGAGTGTG TTAGCTACGT CAACAGCAAC
    ATGGAGAGCG CTGTGGGCTC CCTCTACGTC AGGGAGGCCT TCCCTGGAGA CAGCAAGGAC
    GCGGTGGGTG CTCGGCACTG GGACTGCTCC GCACCCCTGC CCATGGTCCA TTGCCCAGGG
    CCAGCAGGGA CGGGGCTTTG GACCAGACCC CTGCAGCGAC CCCGGAGCTG GTCATGCAGG
    ACAAGGGCCA TGCACTCCCC CCAGGTCAGA GAGCTCATCG ACAAGGTACG GGCGGTATTC
    GTGGAGACCC TGGATGAGCT GGGCTGGATG GACGAGGAGT CCAAGAAGAA GGCCCAGGAG
    AAGGCCATGA ACATCCGCGA GCAGATTGGG TACCCAGACT ACATTCTGGA GGAAAGAAAC
    AAGCACCTGG ACGAGGAGTA CTCCAATCTG AACTTCTCGG AGGACCAGTA CTTTGAAAAC
    GGTCTCCAGA ACCTCAAGGC TGGCGCCCAG CGGAGCCTCA AGAAGCTGCG GGAGAAAGTA
    GACCAGAACC TGGGCCCCAA GCAGGGGAGC CGCATTCAGC CAGCAGGGGG CCAGGACGGA
    GGACTCCCAG GCACGGGCTT GGGTGACCTG CCCGTTCATC ACAGCTGGAT CATTGGGGCG
    GCCGTGGTTA ATGCATTCTA CTCCCCGAAC CGAAACCAAA TAGTGTTCCC TGCTGGGATC
    CTACAGCCTC CATTCTTCAG CAAACATCAG CCTCAGGCCT TGAACTTCGG GGGCATTGGG
    ATGGTGATTG GGCATGAGAT CACCCACGGC TTTGACGACA ACGGCCGTAA CTTTGACAAG
    AATGGGAACA TGCTGGATTG GTGGAGCAAT TTCTCAGCCC AGCACTTCCG GGAGCAGTCG
    GAGTGCATGG TCCACCAGTA CGGGAACTAC TCCTGGGACC TGGCGGACAA CCAAAACGTG
    AACGGGTTCA GCACACTCGG GGAAAACATC GCCGACAACG GAGGGGTGCG GCAGGCATAC
    AAGGCTTACC TCAAGTGGAT GGCAGAAGGC GGCAAGGACC AGCAGCTGCC AGGGCTGGGA
    CTGACCTACA ACCAGCTGTT CTTTATCAAC TATGCCCAGG TGTGGTGTGG GTCCTACCGG
    CCTGAGTTTG CCATACAGTC CATCAAGACC GACGTCCATA GCCCCCTGAA GTACAGGGTG
    CTGGGCTCGC TGCAGAACCT GGCAGCCTTC TCGGAGGCCT TCCACTGTGC CCAGGGCAGC
    CCGATGCACC CGCACCAGCG ATGCCGCGTC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274642.1
    CDS226..2841
    Translation

    Target ORF information:

    RefSeq Version XM_022418934.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418934.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGGCCCCAA GTGGGAGCTG GAGCAGCAGC TGGCCTTGAT GAACACACAG
    TTCAACAGGC GTGTCCTCAT TGACCTCTTC ATCTGGAACG ATGACCAGAA TTCTAGCCGG
    CATATCATCT ACATAGACCA GCCCACCTTG GGCATGCCAT CCAGAGAGTA CTATTTCAAC
    GAGGGCAGCA ACCGGAAGGT GCGGGAAGCC TACCTGCAGT TCATGATGTC GGTGGCCATG
    ATGCTGCGGG CAGACATGAA CCTCCCAGAG AACAGCTACC TGGTGCGGGA GGACATGGTG
    CAGGTGCTGG AGCTGGAGAC GCAGCTGGCA AACGCCACGG CCCCCCAGGA GGAGAGGCAC
    GATGTCACTG CCCTATATCA TAGGATGGGC CTGAAGGAAC TCCAGAGCAA GTTTAGCCTG
    AAGGGGTTTA ACTGGACCCT CTTCATACAA TCTGTGCTGT CCTCTGTCAA AATCAAACTG
    TTGCCAGATG AAGAAGTGGT GGTCTATGGC ATCCCCTACC TTCAGAACCT TGAAGAAATC
    ATCGATGTCT ACTCAGCCAG GACCATGCAG AACTACCTGG TCTGGCGCCT GGTGCTGGAC
    CGCATTAGTA GCCTGAGCCA GCGATTCAAG GATGCACGGG CAAACTACCG CAAGGCGCTG
    TACGGCACAA CCGTGGAAGA GGTGCGCTGG CGAGAGTGTG TTAGCTACGT CAACAGCAAC
    ATGGAGAGCG CTGTGGGCTC CCTCTACGTC AGGGAGGCCT TCCCTGGAGA CAGCAAGGAC
    GCGGTGGGTG CTCGGCACTG GGACTGCTCC GCACCCCTGC CCATGGTCCA TTGCCCAGGG
    CCAGCAGGGA CGGGGCTTTG GACCAGACCC CTGCAGCGAC CCCGGAGCTG GTCATGCAGG
    ACAAGGGCCA TGCACTCCCC CCAGGTCAGA GAGCTCATCG ACAAGGTACG GGCGGTATTC
    GTGGAGACCC TGGATGAGCT GGGCTGGATG GACGAGGAGT CCAAGAAGAA GGCCCAGGAG
    AAGGCCATGA ACATCCGCGA GCAGATTGGG TACCCAGACT ACATTCTGGA GGAAAGAAAC
    AAGCACCTGG ACGAGGAGTA CTCCAATCTG AACTTCTCGG AGGACCAGTA CTTTGAAAAC
    GGTCTCCAGA ACCTCAAGGC TGGCGCCCAG CGGAGCCTCA AGAAGCTGCG GGAGAAAGTA
    GACCAGAACC TGGGCCCCAA GCAGGGGAGC CGCATTCAGC CAGCAGGGGG CCAGGACGGA
    GGACTCCCAG GCACGGGCTT GGGTGACCTG CCCGTTCATC ACAGCTGGAT CATTGGGGCG
    GCCGTGGTTA ATGCATTCTA CTCCCCGAAC CGAAACCAAA TAGTGTTCCC TGCTGGGATC
    CTACAGCCTC CATTCTTCAG CAAACATCAG CCTCAGGCCT TGAACTTCGG GGGCATTGGG
    ATGGTGATTG GGCATGAGAT CACCCACGGC TTTGACGACA ACGGCCGTAA CTTTGACAAG
    AATGGGAACA TGCTGGATTG GTGGAGCAAT TTCTCAGCCC AGCACTTCCG GGAGCAGTCG
    GAGTGCATGG TCCACCAGTA CGGGAACTAC TCCTGGGACC TGGCGGACAA CCAAAACGTG
    AACGGGTTCA GCACACTCGG GGAAAACATC GCCGACAACG GAGGGGTGCG GCAGGCATAC
    AAGGCTTACC TCAAGTGGAT GGCAGAAGGC GGCAAGGACC AGCAGCTGCC AGGGCTGGGA
    CTGACCTACA ACCAGCTGTT CTTTATCAAC TATGCCCAGG TGTGGTGTGG GTCCTACCGG
    CCTGAGTTTG CCATACAGTC CATCAAGACC GACGTCCATA GCCCCCTGAA GTACAGGGTG
    CTGGGCTCGC TGCAGAACCT GGCAGCCTTC TCGGAGGCCT TCCACTGTGC CCAGGGCAGC
    CCGATGCACC CGCACCAGCG ATGCCGCGTC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288365
    Clone ID Related Accession (Same CDS sequence) XM_022418935.1
    Accession Version XM_022418935.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGGGG TGCTAGAGAA TGCCACCACC
    CAGGACAGGC CGGCTGTGCA GAAGGCCAAG ATGCTATACC GCTCCTGCAT GAACCAGAGC
    GTGATAGAGA AACGAGACTC ACAGCCCCTG CTAAACATCC TGGACGTGAT GGGAGGCTGG
    CCAGTGACCA TGGATAAGTG GAACCAGAGC GTAGCCCGAG GCACTGACTC CCCAGGCCCC
    AAGTGGGAGC TGGAGCAGCA GCTGGCCTTG ATGAACACAC AGTTCAACAG GCGTGTCCTC
    ATTGACCTCT TCATCTGGAA CGATGACCAG AATTCTAGCC GGCATATCAT CTACATAGAC
    CAGCCCACCT TGGGCATGCC ATCCAGAGAG TACTATTTCA ACGAGGGCAG CAACCGGAAG
    GTGCGGGAAG CCTACCTGCA GTTCATGATG TCGGTGGCCA TGATGCTGCG GGCAGACATG
    AACCTCCCAG AGAACAGCTA CCTGGTGCGG GAGGACATGG TGCAGGTGCT GGAGCTGGAG
    ACGCAGCTGG CAAACGCCAC GGCCCCCCAG GAGGAGAGGC ACGATGTCAC TGCCCTATAT
    CATAGGATGG GCCTGAAGGA ACTCCAGAGC AAGTTTAGCC TGAAGGGGTT TAACTGGACC
    CTCTTCATAC AATCTGTGCT GTCCTCTGTC AAAATCAAAC TGTTGCCAGA TGAAGAAGTG
    GTGGTCTATG GCATCCCCTA CCTTCAGAAC CTTGAAGAAA TCATCGATGT CTACTCAGCC
    AGGACCATGC AGAACTACCT GGTCTGGCGC CTGGTGCTGG ACCGCATTAG TAGCCTGAGC
    CAGCGATTCA AGGATGCACG GGCAAACTAC CGCAAGGCGC TGTACGGCAC AACCGTGGAA
    GAGGTGCGCT GGCGAGAGTG TGTTAGCTAC GTCAACAGCA ACATGGAGAG CGCTGTGGGC
    TCCCTCTACG TCAGGGAGGC CTTCCCTGGA GACAGCAAGG ACGCGGTGGG TGCTCGGCAC
    TGGGACTGCT CCGCACCCCT GCCCATGGTC CATTGCCCAG GGCCAGCAGG GACGGGGCTT
    TGGACCAGAC CCCTGCAGCG ACCCCGGAGC TGGTCATGCA GGACAAGGGC CATGCACTCC
    CCCCAGGTCA GAGAGCTCAT CGACAAGGTA CGGGCGGTAT TCGTGGAGAC CCTGGATGAG
    CTGGGCTGGA TGGACGAGGA GTCCAAGAAG AAGGCCCAGG AGAAGGCCAT GAACATCCGC
    GAGCAGATTG GGTACCCAGA CTACATTCTG GAGGAAAGAA ACAAGCACCT GGACGAGGAG
    TACTCCAATC TGAACTTCTC GGAGGACCAG TACTTTGAAA ACGGTCTCCA GAACCTCAAG
    GCTGGCGCCC AGCGGAGCCT CAAGAAGCTG CGGGAGAAAG TAGACCAGAA CCTGGGCCCC
    AAGCAGGGGA GCCGCATTCA GCCAGCAGGG GGCCAGGACG GAGGACTCCC AGGCACGGGC
    TTGGGTGACC TGCCCGTTCA TCACAGCTGG ATCATTGGGG CGGCCGTGGT TAATGCATTC
    TACTCCCCGA ACCGAAACCA AATAGTGTTC CCTGCTGGGA TCCTACAGCC TCCATTCTTC
    AGCAAACATC AGCCTCAGGC CTTGAACTTC GGGGGCATTG GGATGGTGAT TGGGCATGAG
    ATCACCCACG GCTTTGACGA CAACGGCCGT AACTTTGACA AGAATGGGAA CATGCTGGAT
    TGGTGGAGCA ATTTCTCAGC CCAGCACTTC CGGGAGCAGT CGGAGTGCAT GGTCCACCAG
    TACGGGAACT ACTCCTGGGA CCTGGCGGAC AACCAAAACG TGAACGGGTT CAGCACACTC
    GGGGAAAACA TCGCCGACAA CGGAGGGGTG CGGCAGGCAT ACAAGGCTTA CCTCAAGTGG
    ATGGCAGAAG GCGGCAAGGA CCAGCAGCTG CCAGGGCTGG GACTGACCTA CAACCAGCTG
    TTCTTTATCA ACTATGCCCA GGTGTGGTGT GGGTCCTACC GGCCTGAGTT TGCCATACAG
    TCCATCAAGA CCGACGTCCA TAGCCCCCTG AAGTACAGGG TGCTGGGCTC GCTGCAGAAC
    CTGGCAGCCT TCTCGGAGGC CTTCCACTGT GCCCAGGGCA GCCCGATGCA CCCGCACCAG
    CGATGCCGCG TCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274643.1
    CDS217..2814
    Translation

    Target ORF information:

    RefSeq Version XM_022418935.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418935.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGGGG TGCTAGAGAA TGCCACCACC
    CAGGACAGGC CGGCTGTGCA GAAGGCCAAG ATGCTATACC GCTCCTGCAT GAACCAGAGC
    GTGATAGAGA AACGAGACTC ACAGCCCCTG CTAAACATCC TGGACGTGAT GGGAGGCTGG
    CCAGTGACCA TGGATAAGTG GAACCAGAGC GTAGCCCGAG GCACTGACTC CCCAGGCCCC
    AAGTGGGAGC TGGAGCAGCA GCTGGCCTTG ATGAACACAC AGTTCAACAG GCGTGTCCTC
    ATTGACCTCT TCATCTGGAA CGATGACCAG AATTCTAGCC GGCATATCAT CTACATAGAC
    CAGCCCACCT TGGGCATGCC ATCCAGAGAG TACTATTTCA ACGAGGGCAG CAACCGGAAG
    GTGCGGGAAG CCTACCTGCA GTTCATGATG TCGGTGGCCA TGATGCTGCG GGCAGACATG
    AACCTCCCAG AGAACAGCTA CCTGGTGCGG GAGGACATGG TGCAGGTGCT GGAGCTGGAG
    ACGCAGCTGG CAAACGCCAC GGCCCCCCAG GAGGAGAGGC ACGATGTCAC TGCCCTATAT
    CATAGGATGG GCCTGAAGGA ACTCCAGAGC AAGTTTAGCC TGAAGGGGTT TAACTGGACC
    CTCTTCATAC AATCTGTGCT GTCCTCTGTC AAAATCAAAC TGTTGCCAGA TGAAGAAGTG
    GTGGTCTATG GCATCCCCTA CCTTCAGAAC CTTGAAGAAA TCATCGATGT CTACTCAGCC
    AGGACCATGC AGAACTACCT GGTCTGGCGC CTGGTGCTGG ACCGCATTAG TAGCCTGAGC
    CAGCGATTCA AGGATGCACG GGCAAACTAC CGCAAGGCGC TGTACGGCAC AACCGTGGAA
    GAGGTGCGCT GGCGAGAGTG TGTTAGCTAC GTCAACAGCA ACATGGAGAG CGCTGTGGGC
    TCCCTCTACG TCAGGGAGGC CTTCCCTGGA GACAGCAAGG ACGCGGTGGG TGCTCGGCAC
    TGGGACTGCT CCGCACCCCT GCCCATGGTC CATTGCCCAG GGCCAGCAGG GACGGGGCTT
    TGGACCAGAC CCCTGCAGCG ACCCCGGAGC TGGTCATGCA GGACAAGGGC CATGCACTCC
    CCCCAGGTCA GAGAGCTCAT CGACAAGGTA CGGGCGGTAT TCGTGGAGAC CCTGGATGAG
    CTGGGCTGGA TGGACGAGGA GTCCAAGAAG AAGGCCCAGG AGAAGGCCAT GAACATCCGC
    GAGCAGATTG GGTACCCAGA CTACATTCTG GAGGAAAGAA ACAAGCACCT GGACGAGGAG
    TACTCCAATC TGAACTTCTC GGAGGACCAG TACTTTGAAA ACGGTCTCCA GAACCTCAAG
    GCTGGCGCCC AGCGGAGCCT CAAGAAGCTG CGGGAGAAAG TAGACCAGAA CCTGGGCCCC
    AAGCAGGGGA GCCGCATTCA GCCAGCAGGG GGCCAGGACG GAGGACTCCC AGGCACGGGC
    TTGGGTGACC TGCCCGTTCA TCACAGCTGG ATCATTGGGG CGGCCGTGGT TAATGCATTC
    TACTCCCCGA ACCGAAACCA AATAGTGTTC CCTGCTGGGA TCCTACAGCC TCCATTCTTC
    AGCAAACATC AGCCTCAGGC CTTGAACTTC GGGGGCATTG GGATGGTGAT TGGGCATGAG
    ATCACCCACG GCTTTGACGA CAACGGCCGT AACTTTGACA AGAATGGGAA CATGCTGGAT
    TGGTGGAGCA ATTTCTCAGC CCAGCACTTC CGGGAGCAGT CGGAGTGCAT GGTCCACCAG
    TACGGGAACT ACTCCTGGGA CCTGGCGGAC AACCAAAACG TGAACGGGTT CAGCACACTC
    GGGGAAAACA TCGCCGACAA CGGAGGGGTG CGGCAGGCAT ACAAGGCTTA CCTCAAGTGG
    ATGGCAGAAG GCGGCAAGGA CCAGCAGCTG CCAGGGCTGG GACTGACCTA CAACCAGCTG
    TTCTTTATCA ACTATGCCCA GGTGTGGTGT GGGTCCTACC GGCCTGAGTT TGCCATACAG
    TCCATCAAGA CCGACGTCCA TAGCCCCCTG AAGTACAGGG TGCTGGGCTC GCTGCAGAAC
    CTGGCAGCCT TCTCGGAGGC CTTCCACTGT GCCCAGGGCA GCCCGATGCA CCCGCACCAG
    CGATGCCGCG TCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288366
    Clone ID Related Accession (Same CDS sequence) XM_022418936.1
    Accession Version XM_022418936.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGGGG TGCTAGAGAA TGCCACCACC
    CAGGACAGGC CGGCTGTGCA GAAGGCCAAG ATGCTATACC GCTCCTGCAT GAACCAGAGC
    GTGATAGAGA AACGAGACTC ACAGCCCCTG CTAAACATCC TGGACGTGAT GGGAGGCTGG
    CCAGTGACCA TGGATAAGTG GAACCAGAGC GTAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTGGGT GCTCGGCACT GGGACTGCTC CGCACCCCTG
    CCCATGGTCC ATTGCCCAGG GCCAGCAGGG ACGGGGCTTT GGACCAGACC CCTGCAGCGA
    CCCCGGAGCT GGTCATGCAG GACAAGGGCC ATGCACTCCC CCCAGGTCAG AGAGCTCATC
    GACAAGGTAC GGGCGGTATT CGTGGAGACC CTGGATGAGC TGGGCTGGAT GGACGAGGAG
    TCCAAGAAGA AGGCCCAGGA GAAGGCCATG AACATCCGCG AGCAGATTGG GTACCCAGAC
    TACATTCTGG AGGAAAGAAA CAAGCACCTG GACGAGGAGT ACTCCAATCT GAACTTCTCG
    GAGGACCAGT ACTTTGAAAA CGGTCTCCAG AACCTCAAGG CTGGCGCCCA GCGGAGCCTC
    AAGAAGCTGC GGGAGAAAGT AGACCAGAAC CTGGGCCCCA AGCAGGGGAG CCGCATTCAG
    CCAGCAGGGG GCCAGGACGG AGGACTCCCA GGCACGGGCT TGGGTGACCT GCCCGTTCAT
    CACAGCTGGA TCATTGGGGC GGCCGTGGTT AATGCATTCT ACTCCCCGAA CCGAAACCAA
    ATAGTGTTCC CTGCTGGGAT CCTACAGCCT CCATTCTTCA GCAAACATCA GCCTCAGGCC
    TTGAACTTCG GGGGCATTGG GATGGTGATT GGGCATGAGA TCACCCACGG CTTTGACGAC
    AACGGCCGTA ACTTTGACAA GAATGGGAAC ATGCTGGATT GGTGGAGCAA TTTCTCAGCC
    CAGCACTTCC GGGAGCAGTC GGAGTGCATG GTCCACCAGT ACGGGAACTA CTCCTGGGAC
    CTGGCGGACA ACCAAAACGT GAACGGGTTC AGCACACTCG GGGAAAACAT CGCCGACAAC
    GGAGGGGTGC GGCAGGCATA CAAGGCTTAC CTCAAGTGGA TGGCAGAAGG CGGCAAGGAC
    CAGCAGCTGC CAGGGCTGGG ACTGACCTAC AACCAGCTGT TCTTTATCAA CTATGCCCAG
    GTGTGGTGTG GGTCCTACCG GCCTGAGTTT GCCATACAGT CCATCAAGAC CGACGTCCAT
    AGCCCCCTGA AGTACAGGGT GCTGGGCTCG CTGCAGAACC TGGCAGCCTT CTCGGAGGCC
    TTCCACTGTG CCCAGGGCAG CCCGATGCAC CCGCACCAGC GATGCCGCGT CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274644.1
    CDS221..2797
    Translation

    Target ORF information:

    RefSeq Version XM_022418936.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418936.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGGGG TGCTAGAGAA TGCCACCACC
    CAGGACAGGC CGGCTGTGCA GAAGGCCAAG ATGCTATACC GCTCCTGCAT GAACCAGAGC
    GTGATAGAGA AACGAGACTC ACAGCCCCTG CTAAACATCC TGGACGTGAT GGGAGGCTGG
    CCAGTGACCA TGGATAAGTG GAACCAGAGC GTAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTGGGT GCTCGGCACT GGGACTGCTC CGCACCCCTG
    CCCATGGTCC ATTGCCCAGG GCCAGCAGGG ACGGGGCTTT GGACCAGACC CCTGCAGCGA
    CCCCGGAGCT GGTCATGCAG GACAAGGGCC ATGCACTCCC CCCAGGTCAG AGAGCTCATC
    GACAAGGTAC GGGCGGTATT CGTGGAGACC CTGGATGAGC TGGGCTGGAT GGACGAGGAG
    TCCAAGAAGA AGGCCCAGGA GAAGGCCATG AACATCCGCG AGCAGATTGG GTACCCAGAC
    TACATTCTGG AGGAAAGAAA CAAGCACCTG GACGAGGAGT ACTCCAATCT GAACTTCTCG
    GAGGACCAGT ACTTTGAAAA CGGTCTCCAG AACCTCAAGG CTGGCGCCCA GCGGAGCCTC
    AAGAAGCTGC GGGAGAAAGT AGACCAGAAC CTGGGCCCCA AGCAGGGGAG CCGCATTCAG
    CCAGCAGGGG GCCAGGACGG AGGACTCCCA GGCACGGGCT TGGGTGACCT GCCCGTTCAT
    CACAGCTGGA TCATTGGGGC GGCCGTGGTT AATGCATTCT ACTCCCCGAA CCGAAACCAA
    ATAGTGTTCC CTGCTGGGAT CCTACAGCCT CCATTCTTCA GCAAACATCA GCCTCAGGCC
    TTGAACTTCG GGGGCATTGG GATGGTGATT GGGCATGAGA TCACCCACGG CTTTGACGAC
    AACGGCCGTA ACTTTGACAA GAATGGGAAC ATGCTGGATT GGTGGAGCAA TTTCTCAGCC
    CAGCACTTCC GGGAGCAGTC GGAGTGCATG GTCCACCAGT ACGGGAACTA CTCCTGGGAC
    CTGGCGGACA ACCAAAACGT GAACGGGTTC AGCACACTCG GGGAAAACAT CGCCGACAAC
    GGAGGGGTGC GGCAGGCATA CAAGGCTTAC CTCAAGTGGA TGGCAGAAGG CGGCAAGGAC
    CAGCAGCTGC CAGGGCTGGG ACTGACCTAC AACCAGCTGT TCTTTATCAA CTATGCCCAG
    GTGTGGTGTG GGTCCTACCG GCCTGAGTTT GCCATACAGT CCATCAAGAC CGACGTCCAT
    AGCCCCCTGA AGTACAGGGT GCTGGGCTCG CTGCAGAACC TGGCAGCCTT CTCGGAGGCC
    TTCCACTGTG CCCAGGGCAG CCCGATGCAC CCGCACCAGC GATGCCGCGT CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288367
    Clone ID Related Accession (Same CDS sequence) XM_022418937.1
    Accession Version XM_022418937.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTGGGT GCTCGGCACT GGGACTGCTC CGCACCCCTG
    CCCATGGTCC ATTGCCCAGG GCCAGCAGGG ACGGGGCTTT GGACCAGACC CCTGCAGCGA
    CCCCGGAGCT GGTCATGCAG GACAAGGGCC ATGCACTCCC CCCAGGTCAG AGAGCTCATC
    GACAAGGTAC GGGCGGTATT CGTGGAGACC CTGGATGAGC TGGGCTGGAT GGACGAGGAG
    TCCAAGAAGA AGGCCCAGGA GAAGGCCATG AACATCCGCG AGCAGATTGG GTACCCAGAC
    TACATTCTGG AGGAAAGAAA CAAGCACCTG GACGAGGAGT ACTCCAATCT GAACTTCTCG
    GAGGACCAGT ACTTTGAAAA CGGTCTCCAG AACCTCAAGG CTGGCGCCCA GCGGAGCCTC
    AAGAAGCTGC GGGAGAAAGT AGACCAGAAC CTGGGCCCCA AGCAGGGGAG CCGCATTCAG
    CCAGCAGGGG GCCAGGACGG AGGACTCCCA GGCACGGGCT TGGGTGACCT GCCCGTTCAT
    CACAGCTGGA TCATTGGGGC GGCCGTGGTT AATGCATTCT ACTCCCCGAA CCGAAACCAA
    ATAGTGTTCC CTGCTGGGAT CCTACAGCCT CCATTCTTCA GCAAACATCA GCCTCAGGCC
    TTGAACTTCG GGGGCATTGG GATGGTGATT GGGCATGAGA TCACCCACGG CTTTGACGAC
    AACGGCCGTA ACTTTGACAA GAATGGGAAC ATGCTGGATT GGTGGAGCAA TTTCTCAGCC
    CAGCACTTCC GGGAGCAGTC GGAGTGCATG GTCCACCAGT ACGGGAACTA CTCCTGGGAC
    CTGGCGGACA ACCAAAACGT GAACGGGTTC AGCACACTCG GGGAAAACAT CGCCGACAAC
    GGAGGGGTGC GGCAGGCATA CAAGGCTTAC CTCAAGTGGA TGGCAGAAGG CGGCAAGGAC
    CAGCAGCTGC CAGGGCTGGG ACTGACCTAC AACCAGCTGT TCTTTATCAA CTATGCCCAG
    GCCCGCCCCC CAGTCCTGCA GCCATGCCCC TCGTGTGCCC ACAGGTGTGG TGTGGGTCCT
    ACCGGCCTGA GTTTGCCATA CAGTCCATCA AGACCGACGT CCATAGCCCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274645.1
    CDS226..2799
    Translation

    Target ORF information:

    RefSeq Version XM_022418937.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418937.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTGGGT GCTCGGCACT GGGACTGCTC CGCACCCCTG
    CCCATGGTCC ATTGCCCAGG GCCAGCAGGG ACGGGGCTTT GGACCAGACC CCTGCAGCGA
    CCCCGGAGCT GGTCATGCAG GACAAGGGCC ATGCACTCCC CCCAGGTCAG AGAGCTCATC
    GACAAGGTAC GGGCGGTATT CGTGGAGACC CTGGATGAGC TGGGCTGGAT GGACGAGGAG
    TCCAAGAAGA AGGCCCAGGA GAAGGCCATG AACATCCGCG AGCAGATTGG GTACCCAGAC
    TACATTCTGG AGGAAAGAAA CAAGCACCTG GACGAGGAGT ACTCCAATCT GAACTTCTCG
    GAGGACCAGT ACTTTGAAAA CGGTCTCCAG AACCTCAAGG CTGGCGCCCA GCGGAGCCTC
    AAGAAGCTGC GGGAGAAAGT AGACCAGAAC CTGGGCCCCA AGCAGGGGAG CCGCATTCAG
    CCAGCAGGGG GCCAGGACGG AGGACTCCCA GGCACGGGCT TGGGTGACCT GCCCGTTCAT
    CACAGCTGGA TCATTGGGGC GGCCGTGGTT AATGCATTCT ACTCCCCGAA CCGAAACCAA
    ATAGTGTTCC CTGCTGGGAT CCTACAGCCT CCATTCTTCA GCAAACATCA GCCTCAGGCC
    TTGAACTTCG GGGGCATTGG GATGGTGATT GGGCATGAGA TCACCCACGG CTTTGACGAC
    AACGGCCGTA ACTTTGACAA GAATGGGAAC ATGCTGGATT GGTGGAGCAA TTTCTCAGCC
    CAGCACTTCC GGGAGCAGTC GGAGTGCATG GTCCACCAGT ACGGGAACTA CTCCTGGGAC
    CTGGCGGACA ACCAAAACGT GAACGGGTTC AGCACACTCG GGGAAAACAT CGCCGACAAC
    GGAGGGGTGC GGCAGGCATA CAAGGCTTAC CTCAAGTGGA TGGCAGAAGG CGGCAAGGAC
    CAGCAGCTGC CAGGGCTGGG ACTGACCTAC AACCAGCTGT TCTTTATCAA CTATGCCCAG
    GCCCGCCCCC CAGTCCTGCA GCCATGCCCC TCGTGTGCCC ACAGGTGTGG TGTGGGTCCT
    ACCGGCCTGA GTTTGCCATA CAGTCCATCA AGACCGACGT CCATAGCCCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288368
    Clone ID Related Accession (Same CDS sequence) XM_022418938.1
    Accession Version XM_022418938.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTGGGT GCTCGGCACT GGGACTGCTC CGCACCCCTG
    CCCATGGTCC ATTGCCCAGG GCCAGCAGGG ACGGGGCTTT GGACCAGACC CCTGCAGCGA
    CCCCGGAGCT GGTCATGCAG GACAAGGGCC ATGCACTCCC CCCAGGTCAG AGAGCTCATC
    GACAAGGCCA TGAACATCCG CGAGCAGATT GGGTACCCAG ACTACATTCT GGAGGAAAGA
    AACAAGCACC TGGACGAGGA GTACTCCAAT CTGAACTTCT CGGAGGACCA GTACTTTGAA
    AACGGTCTCC AGAACCTCAA GGCTGGCGCC CAGCGGAGCC TCAAGAAGCT GCGGGAGAAA
    GTAGACCAGA ACCTGGGCCC CAAGCAGGGG AGCCGCATTC AGCCAGCAGG GGGCCAGGAC
    GGAGGACTCC CAGGCACGGG CTTGGGTGAC CTGCCCGTTC ATCACAGCTG GATCATTGGG
    GCGGCCGTGG TTAATGCATT CTACTCCCCG AACCGAAACC AAATAGTGTT CCCTGCTGGG
    ATCCTACAGC CTCCATTCTT CAGCAAACAT CAGCCTCAGG CCTTGAACTT CGGGGGCATT
    GGGATGGTGA TTGGGCATGA GATCACCCAC GGCTTTGACG ACAACGGCCG TAACTTTGAC
    AAGAATGGGA ACATGCTGGA TTGGTGGAGC AATTTCTCAG CCCAGCACTT CCGGGAGCAG
    TCGGAGTGCA TGGTCCACCA GTACGGGAAC TACTCCTGGG ACCTGGCGGA CAACCAAAAC
    GTGAACGGGT TCAGCACACT CGGGGAAAAC ATCGCCGACA ACGGAGGGGT GCGGCAGGCA
    TACAAGGCTT ACCTCAAGTG GATGGCAGAA GGCGGCAAGG ACCAGCAGCT GCCAGGGCTG
    GGACTGACCT ACAACCAGCT GTTCTTTATC AACTATGCCC AGGTGTGGTG TGGGTCCTAC
    CGGCCTGAGT TTGCCATACA GTCCATCAAG ACCGACGTCC ATAGCCCCCT GAAGTACAGG
    GTGCTGGGCT CGCTGCAGAA CCTGGCAGCC TTCTCGGAGG CCTTCCACTG TGCCCAGGGC
    AGCCCGATGC ACCCGCACCA GCGATGCCGC GTCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274646.1
    CDS224..2782
    Translation

    Target ORF information:

    RefSeq Version XM_022418938.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418938.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTGGGT GCTCGGCACT GGGACTGCTC CGCACCCCTG
    CCCATGGTCC ATTGCCCAGG GCCAGCAGGG ACGGGGCTTT GGACCAGACC CCTGCAGCGA
    CCCCGGAGCT GGTCATGCAG GACAAGGGCC ATGCACTCCC CCCAGGTCAG AGAGCTCATC
    GACAAGGCCA TGAACATCCG CGAGCAGATT GGGTACCCAG ACTACATTCT GGAGGAAAGA
    AACAAGCACC TGGACGAGGA GTACTCCAAT CTGAACTTCT CGGAGGACCA GTACTTTGAA
    AACGGTCTCC AGAACCTCAA GGCTGGCGCC CAGCGGAGCC TCAAGAAGCT GCGGGAGAAA
    GTAGACCAGA ACCTGGGCCC CAAGCAGGGG AGCCGCATTC AGCCAGCAGG GGGCCAGGAC
    GGAGGACTCC CAGGCACGGG CTTGGGTGAC CTGCCCGTTC ATCACAGCTG GATCATTGGG
    GCGGCCGTGG TTAATGCATT CTACTCCCCG AACCGAAACC AAATAGTGTT CCCTGCTGGG
    ATCCTACAGC CTCCATTCTT CAGCAAACAT CAGCCTCAGG CCTTGAACTT CGGGGGCATT
    GGGATGGTGA TTGGGCATGA GATCACCCAC GGCTTTGACG ACAACGGCCG TAACTTTGAC
    AAGAATGGGA ACATGCTGGA TTGGTGGAGC AATTTCTCAG CCCAGCACTT CCGGGAGCAG
    TCGGAGTGCA TGGTCCACCA GTACGGGAAC TACTCCTGGG ACCTGGCGGA CAACCAAAAC
    GTGAACGGGT TCAGCACACT CGGGGAAAAC ATCGCCGACA ACGGAGGGGT GCGGCAGGCA
    TACAAGGCTT ACCTCAAGTG GATGGCAGAA GGCGGCAAGG ACCAGCAGCT GCCAGGGCTG
    GGACTGACCT ACAACCAGCT GTTCTTTATC AACTATGCCC AGGTGTGGTG TGGGTCCTAC
    CGGCCTGAGT TTGCCATACA GTCCATCAAG ACCGACGTCC ATAGCCCCCT GAAGTACAGG
    GTGCTGGGCT CGCTGCAGAA CCTGGCAGCC TTCTCGGAGG CCTTCCACTG TGCCCAGGGC
    AGCCCGATGC ACCCGCACCA GCGATGCCGC GTCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288369
    Clone ID Related Accession (Same CDS sequence) XM_022418939.1
    Accession Version XM_022418939.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGCCA TCCAAGAGCC CAAGGAGATG
    GGCGAAATCT GCACCACCCC TGGCTGTGTG ATAGCGGCCT CCAGGATCCT CCAGAACATG
    GACCCGTCCA GGGAGCCCTG TGAAGACTTC TACCAGTACG CATGCGGAGG CTGGCTGCGG
    CGCCACGTGA TCCCCGAGAC CAACTCCCGA TACAGTGTCT TTGACATCCT CCGAGACGAG
    CTGGAGGTCA TCCTCAAAGC TGCCTCGGGC CTCCATCCAG CTTCCCCTCT GCCCGCAGGG
    GTGCTAGAGA ATGCCACCAC CCAGGACAGG CCGGCTGTGC AGAAGGCCAA GATGCTATAC
    CGCTCCTGCA TGAACCAGAG CGTGATAGAG AAACGAGACT CACAGCCCCT GCTAAACATC
    CTGGACGTGA TGGGAGGCTG GCCAGTGACC ATGGATAAGT GGAACCAGAG CGTAGCCCGA
    GGCACTGACT CCCCAGGCCC CAAGTGGGAG CTGGAGCAGC AGCTGGCCTT GATGAACACA
    CAGTTCAACA GGCGTGTCCT CATTGACCTC TTCATCTGGA ACGATGACCA GAATTCTAGC
    CGGCATATCA TCTACATAGA CCAGCCCACC TTGGGCATGC CATCCAGAGA GTACTATTTC
    AACGAGGGCA GCAACCGGAA GGTGCGGGAA GCCTACCTGC AGTTCATGAT GTCGGTGGCC
    ATGATGCTGC GGGCAGACAT GAACCTCCCA GAGAACAGCT ACCTGGTGCG GGAGGACATG
    GTGCAGGTGC TGGAGCTGGA GACGCAGCTG GCAAACGCCA CGGCCCCCCA GGAGGAGAGG
    CACGATGTCA CTGCCCTATA TCATAGGATG GGCCTGAAGG AACTCCAGAG CAAGTTTAGC
    CTGAAGGGGT TTAACTGGAC CCTCTTCATA CAATCTGTGC TGTCCTCTGT CAAAATCAAA
    CTGTTGCCAG ATGAAGAAGT GGTGGTCTAT GGCATCCCCT ACCTTCAGAA CCTTGAAGAA
    ATCATCGATG TCTACTCAGC CAGGACCATG CAGAACTACC TGGTCTGGCG CCTGGTGCTG
    GACCGCATTA GTAGCCTGAG CCAGCGATTC AAGGATGCAC GGGCAAACTA CCGCAAGGCG
    CTGTACGGCA CAACCGTGGA AGAGGTGCGC TGGCGAGAGT GTGTTAGCTA CGTCAACAGC
    AACATGGAGA GCGCTGTGGG CTCCCTCTAC GTCAGGGAGG CCTTCCCTGG AGACAGCAAG
    GACGCGGTGG GTGCTCGGCA CTGGGACTGC TCCGCACCCC TGCCCATGGT CCATTGCCCA
    GGGCCAGCAG GGACGGGGCT TTGGACCAGA CCCCTGCAGC GACCCCGGAG CTGGTCATGC
    AGGACAAGGG CCATGCACTC CCCCCAGGTC AGAGAGCTCA TCGACAAGGT ACGGGCGGTA
    TTCGTGGAGA CCCTGGATGA GCTGGGCTGG ATGGACGAGG AGTCCAAGAA GAAGGCCCAG
    GAGAAGGCCA TGAACATCCG CGAGCAGATT GGGTACCCAG ACTACATTCT GGAGGAAAGA
    AACAAGCACC TGGACGAGGA GTACTCCAAT CTGAACTTCT CGGAGGACCA GTACTTTGAA
    AACGGTCTCC AGAACCTCAA GGCTGGCGCC CAGCGGAGCC TCAAGAAGCT GCGGGAGAAA
    GTAGACCAGA ACCTGGGCCC CAAGCAGGGG AGCCGCATTC AGCCAGCAGG GGGCCAGGAC
    GGAGGACTCC CAGGCACGGG CTTGGGTGAC CTGCCCGTTC ATCACAGCTG GATCATTGGG
    GCGGCCGTGG TTAATGCATT CTACTCCCCG AACCGAAACC AAATAGTGTT CCCTGCTGGG
    ATCCTACAGC CTCCATTCTT CAGCAAACAT CAGCCTCAGG CCTTGAACTT CGGGGGCATT
    GGGATGGTGA TTGGGCATGA GATCACCCAC GGCTTTGACG ACAACGGCCG TAACTTTGAC
    AAGAATGGGA ACATGCTGGA TTGGTGGAGC AATTTCTCAG CCCAGCACTT CCGGGAGCAG
    TCGGAGTGCA TGGTCCACCA GTACGGGAAC TACTCCTGGG ACCTGGCGGA CAACCAAAAC
    GTGAACGGGT TCAGCACACT CGGGGAAAAC ATCGCCGACA ACGGAGGGGT GCGGCAGGCA
    TACAAGGCTT ACCTCAAGTG GATGGCAGAA GGCGGCAAGG ACCAGCAGCT GCCAGGGCTG
    GGACTGACCT ACAACCAGCT GTTCTTTATC AACTATGCCC AGGTGTGGTG TGGGTCCTAC
    CGGCCTGAGT TTGCCATACA GTCCATCAAG ACCGACGTCC ATAGCCCCCT GAAGTACAGG
    GTGCTGGGCT CGCTGCAGAA CCTGGCAGCC TTCTCGGAGG CCTTCCACTG TGCCCAGGGC
    AGCCCGATGC ACCCGCACCA GCGATGCCGC GTCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274647.1
    CDS225..2783
    Translation

    Target ORF information:

    RefSeq Version XM_022418939.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418939.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGCCA TCCAAGAGCC CAAGGAGATG
    GGCGAAATCT GCACCACCCC TGGCTGTGTG ATAGCGGCCT CCAGGATCCT CCAGAACATG
    GACCCGTCCA GGGAGCCCTG TGAAGACTTC TACCAGTACG CATGCGGAGG CTGGCTGCGG
    CGCCACGTGA TCCCCGAGAC CAACTCCCGA TACAGTGTCT TTGACATCCT CCGAGACGAG
    CTGGAGGTCA TCCTCAAAGC TGCCTCGGGC CTCCATCCAG CTTCCCCTCT GCCCGCAGGG
    GTGCTAGAGA ATGCCACCAC CCAGGACAGG CCGGCTGTGC AGAAGGCCAA GATGCTATAC
    CGCTCCTGCA TGAACCAGAG CGTGATAGAG AAACGAGACT CACAGCCCCT GCTAAACATC
    CTGGACGTGA TGGGAGGCTG GCCAGTGACC ATGGATAAGT GGAACCAGAG CGTAGCCCGA
    GGCACTGACT CCCCAGGCCC CAAGTGGGAG CTGGAGCAGC AGCTGGCCTT GATGAACACA
    CAGTTCAACA GGCGTGTCCT CATTGACCTC TTCATCTGGA ACGATGACCA GAATTCTAGC
    CGGCATATCA TCTACATAGA CCAGCCCACC TTGGGCATGC CATCCAGAGA GTACTATTTC
    AACGAGGGCA GCAACCGGAA GGTGCGGGAA GCCTACCTGC AGTTCATGAT GTCGGTGGCC
    ATGATGCTGC GGGCAGACAT GAACCTCCCA GAGAACAGCT ACCTGGTGCG GGAGGACATG
    GTGCAGGTGC TGGAGCTGGA GACGCAGCTG GCAAACGCCA CGGCCCCCCA GGAGGAGAGG
    CACGATGTCA CTGCCCTATA TCATAGGATG GGCCTGAAGG AACTCCAGAG CAAGTTTAGC
    CTGAAGGGGT TTAACTGGAC CCTCTTCATA CAATCTGTGC TGTCCTCTGT CAAAATCAAA
    CTGTTGCCAG ATGAAGAAGT GGTGGTCTAT GGCATCCCCT ACCTTCAGAA CCTTGAAGAA
    ATCATCGATG TCTACTCAGC CAGGACCATG CAGAACTACC TGGTCTGGCG CCTGGTGCTG
    GACCGCATTA GTAGCCTGAG CCAGCGATTC AAGGATGCAC GGGCAAACTA CCGCAAGGCG
    CTGTACGGCA CAACCGTGGA AGAGGTGCGC TGGCGAGAGT GTGTTAGCTA CGTCAACAGC
    AACATGGAGA GCGCTGTGGG CTCCCTCTAC GTCAGGGAGG CCTTCCCTGG AGACAGCAAG
    GACGCGGTGG GTGCTCGGCA CTGGGACTGC TCCGCACCCC TGCCCATGGT CCATTGCCCA
    GGGCCAGCAG GGACGGGGCT TTGGACCAGA CCCCTGCAGC GACCCCGGAG CTGGTCATGC
    AGGACAAGGG CCATGCACTC CCCCCAGGTC AGAGAGCTCA TCGACAAGGT ACGGGCGGTA
    TTCGTGGAGA CCCTGGATGA GCTGGGCTGG ATGGACGAGG AGTCCAAGAA GAAGGCCCAG
    GAGAAGGCCA TGAACATCCG CGAGCAGATT GGGTACCCAG ACTACATTCT GGAGGAAAGA
    AACAAGCACC TGGACGAGGA GTACTCCAAT CTGAACTTCT CGGAGGACCA GTACTTTGAA
    AACGGTCTCC AGAACCTCAA GGCTGGCGCC CAGCGGAGCC TCAAGAAGCT GCGGGAGAAA
    GTAGACCAGA ACCTGGGCCC CAAGCAGGGG AGCCGCATTC AGCCAGCAGG GGGCCAGGAC
    GGAGGACTCC CAGGCACGGG CTTGGGTGAC CTGCCCGTTC ATCACAGCTG GATCATTGGG
    GCGGCCGTGG TTAATGCATT CTACTCCCCG AACCGAAACC AAATAGTGTT CCCTGCTGGG
    ATCCTACAGC CTCCATTCTT CAGCAAACAT CAGCCTCAGG CCTTGAACTT CGGGGGCATT
    GGGATGGTGA TTGGGCATGA GATCACCCAC GGCTTTGACG ACAACGGCCG TAACTTTGAC
    AAGAATGGGA ACATGCTGGA TTGGTGGAGC AATTTCTCAG CCCAGCACTT CCGGGAGCAG
    TCGGAGTGCA TGGTCCACCA GTACGGGAAC TACTCCTGGG ACCTGGCGGA CAACCAAAAC
    GTGAACGGGT TCAGCACACT CGGGGAAAAC ATCGCCGACA ACGGAGGGGT GCGGCAGGCA
    TACAAGGCTT ACCTCAAGTG GATGGCAGAA GGCGGCAAGG ACCAGCAGCT GCCAGGGCTG
    GGACTGACCT ACAACCAGCT GTTCTTTATC AACTATGCCC AGGTGTGGTG TGGGTCCTAC
    CGGCCTGAGT TTGCCATACA GTCCATCAAG ACCGACGTCC ATAGCCCCCT GAAGTACAGG
    GTGCTGGGCT CGCTGCAGAA CCTGGCAGCC TTCTCGGAGG CCTTCCACTG TGCCCAGGGC
    AGCCCGATGC ACCCGCACCA GCGATGCCGC GTCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288370
    Clone ID Related Accession (Same CDS sequence) XM_022418940.1
    Accession Version XM_022418940.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2544bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTGGGT GCTCGGCACT GGGACTGCTC CGCACCCCTG
    CCCATGGTCC ATTGCCCAGG GCCAGCAGGG ACGGGGCTTT GGACCAGACC CCTGCAGCGA
    CCCCGGAGCT GGTCATGCAG GACAAGGGCC ATGCACTCCC CCCAGGTCAG AGAGCTCATC
    GACAAGGTAC GGGCGGTATT CGTGGAGACC CTGGATGAGC TGGGCTGGAT GGACGAGGAG
    TCCAAGAAGA AGGCCCAGGA GAAGGCCATG AACATCCGCG AGCAGATTGG GTACCCAGAC
    TACATTCTGG AGGAAAGAAA CAAGCACCTG GACGAGGAGT ACTCCAATCT GAACTTCTCG
    GAGGACCAGT ACTTTGAAAA CGGTCTCCAG AACCTCAAGG CTGGCGCCCA GCGGAGCCTC
    AAGAAGCTGC GGGAGAAAGT AGACCAGAAC CTCTGGATCA TTGGGGCGGC CGTGGTTAAT
    GCATTCTACT CCCCGAACCG AAACCAAATA GTGTTCCCTG CTGGGATCCT ACAGCCTCCA
    TTCTTCAGCA AACATCAGCC TCAGGCCTTG AACTTCGGGG GCATTGGGAT GGTGATTGGG
    CATGAGATCA CCCACGGCTT TGACGACAAC GGCCGTAACT TTGACAAGAA TGGGAACATG
    CTGGATTGGT GGAGCAATTT CTCAGCCCAG CACTTCCGGG AGCAGTCGGA GTGCATGGTC
    CACCAGTACG GGAACTACTC CTGGGACCTG GCGGACAACC AAAACGTGAA CGGGTTCAGC
    ACACTCGGGG AAAACATCGC CGACAACGGA GGGGTGCGGC AGGCATACAA GGCTTACCTC
    AAGTGGATGG CAGAAGGCGG CAAGGACCAG CAGCTGCCAG GGCTGGGACT GACCTACAAC
    CAGCTGTTCT TTATCAACTA TGCCCAGGTG TGGTGTGGGT CCTACCGGCC TGAGTTTGCC
    ATACAGTCCA TCAAGACCGA CGTCCATAGC CCCCTGAAGT ACAGGGTGCT GGGCTCGCTG
    CAGAACCTGG CAGCCTTCTC GGAGGCCTTC CACTGTGCCC AGGGCAGCCC GATGCACCCG
    CACCAGCGAT GCCGCGTCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274648.1
    CDS214..2757
    Translation

    Target ORF information:

    RefSeq Version XM_022418940.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418940.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTGGGT GCTCGGCACT GGGACTGCTC CGCACCCCTG
    CCCATGGTCC ATTGCCCAGG GCCAGCAGGG ACGGGGCTTT GGACCAGACC CCTGCAGCGA
    CCCCGGAGCT GGTCATGCAG GACAAGGGCC ATGCACTCCC CCCAGGTCAG AGAGCTCATC
    GACAAGGTAC GGGCGGTATT CGTGGAGACC CTGGATGAGC TGGGCTGGAT GGACGAGGAG
    TCCAAGAAGA AGGCCCAGGA GAAGGCCATG AACATCCGCG AGCAGATTGG GTACCCAGAC
    TACATTCTGG AGGAAAGAAA CAAGCACCTG GACGAGGAGT ACTCCAATCT GAACTTCTCG
    GAGGACCAGT ACTTTGAAAA CGGTCTCCAG AACCTCAAGG CTGGCGCCCA GCGGAGCCTC
    AAGAAGCTGC GGGAGAAAGT AGACCAGAAC CTCTGGATCA TTGGGGCGGC CGTGGTTAAT
    GCATTCTACT CCCCGAACCG AAACCAAATA GTGTTCCCTG CTGGGATCCT ACAGCCTCCA
    TTCTTCAGCA AACATCAGCC TCAGGCCTTG AACTTCGGGG GCATTGGGAT GGTGATTGGG
    CATGAGATCA CCCACGGCTT TGACGACAAC GGCCGTAACT TTGACAAGAA TGGGAACATG
    CTGGATTGGT GGAGCAATTT CTCAGCCCAG CACTTCCGGG AGCAGTCGGA GTGCATGGTC
    CACCAGTACG GGAACTACTC CTGGGACCTG GCGGACAACC AAAACGTGAA CGGGTTCAGC
    ACACTCGGGG AAAACATCGC CGACAACGGA GGGGTGCGGC AGGCATACAA GGCTTACCTC
    AAGTGGATGG CAGAAGGCGG CAAGGACCAG CAGCTGCCAG GGCTGGGACT GACCTACAAC
    CAGCTGTTCT TTATCAACTA TGCCCAGGTG TGGTGTGGGT CCTACCGGCC TGAGTTTGCC
    ATACAGTCCA TCAAGACCGA CGTCCATAGC CCCCTGAAGT ACAGGGTGCT GGGCTCGCTG
    CAGAACCTGG CAGCCTTCTC GGAGGCCTTC CACTGTGCCC AGGGCAGCCC GATGCACCCG
    CACCAGCGAT GCCGCGTCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288371
    Clone ID Related Accession (Same CDS sequence) XM_022418941.1
    Accession Version XM_022418941.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTCAGA GAGCTCATCG ACAAGGTACG GGCGGTATTC
    GTGGAGACCC TGGATGAGCT GGGCTGGATG GACGAGGAGT CCAAGAAGAA GGCCCAGGAG
    AAGGCCATGA ACATCCGCGA GCAGATTGGG TACCCAGACT ACATTCTGGA GGAAAGAAAC
    AAGCACCTGG ACGAGGAGTA CTCCAATCTG AACTTCTCGG AGGACCAGTA CTTTGAAAAC
    GGTCTCCAGA ACCTCAAGGC TGGCGCCCAG CGGAGCCTCA AGAAGCTGCG GGAGAAAGTA
    GACCAGAACC TGGGCCCCAA GCAGGGGAGC CGCATTCAGC CAGCAGGGGG CCAGGACGGA
    GGACTCCCAG GCACGGGCTT GGGTGACCTG CCCGTTCATC ACAGCTGGAT CATTGGGGCG
    GCCGTGGTTA ATGCATTCTA CTCCCCGAAC CGAAACCAAA TAGTGTTCCC TGCTGGGATC
    CTACAGCCTC CATTCTTCAG CAAACATCAG CCTCAGGCCT TGAACTTCGG GGGCATTGGG
    ATGGTGATTG GGCATGAGAT CACCCACGGC TTTGACGACA ACGGCCGTAA CTTTGACAAG
    AATGGGAACA TGCTGGATTG GTGGAGCAAT TTCTCAGCCC AGCACTTCCG GGAGCAGTCG
    GAGTGCATGG TCCACCAGTA CGGGAACTAC TCCTGGGACC TGGCGGACAA CCAAAACGTG
    AACGGGTTCA GCACACTCGG GGAAAACATC GCCGACAACG GAGGGGTGCG GCAGGCATAC
    AAGGCTTACC TCAAGTGGAT GGCAGAAGGC GGCAAGGACC AGCAGCTGCC AGGGCTGGGA
    CTGACCTACA ACCAGCTGTT CTTTATCAAC TATGCCCAGG TGTGGTGTGG GTCCTACCGG
    CCTGAGTTTG CCATACAGTC CATCAAGACC GACGTCCATA GCCCCCTGAA GTACAGGGTG
    CTGGGCTCGC TGCAGAACCT GGCAGCCTTC TCGGAGGCCT TCCACTGTGC CCAGGGCAGC
    CCGATGCACC CGCACCAGCG ATGCCGCGTC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274649.1
    CDS213..2708
    Translation

    Target ORF information:

    RefSeq Version XM_022418941.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418941.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTCAGA GAGCTCATCG ACAAGGTACG GGCGGTATTC
    GTGGAGACCC TGGATGAGCT GGGCTGGATG GACGAGGAGT CCAAGAAGAA GGCCCAGGAG
    AAGGCCATGA ACATCCGCGA GCAGATTGGG TACCCAGACT ACATTCTGGA GGAAAGAAAC
    AAGCACCTGG ACGAGGAGTA CTCCAATCTG AACTTCTCGG AGGACCAGTA CTTTGAAAAC
    GGTCTCCAGA ACCTCAAGGC TGGCGCCCAG CGGAGCCTCA AGAAGCTGCG GGAGAAAGTA
    GACCAGAACC TGGGCCCCAA GCAGGGGAGC CGCATTCAGC CAGCAGGGGG CCAGGACGGA
    GGACTCCCAG GCACGGGCTT GGGTGACCTG CCCGTTCATC ACAGCTGGAT CATTGGGGCG
    GCCGTGGTTA ATGCATTCTA CTCCCCGAAC CGAAACCAAA TAGTGTTCCC TGCTGGGATC
    CTACAGCCTC CATTCTTCAG CAAACATCAG CCTCAGGCCT TGAACTTCGG GGGCATTGGG
    ATGGTGATTG GGCATGAGAT CACCCACGGC TTTGACGACA ACGGCCGTAA CTTTGACAAG
    AATGGGAACA TGCTGGATTG GTGGAGCAAT TTCTCAGCCC AGCACTTCCG GGAGCAGTCG
    GAGTGCATGG TCCACCAGTA CGGGAACTAC TCCTGGGACC TGGCGGACAA CCAAAACGTG
    AACGGGTTCA GCACACTCGG GGAAAACATC GCCGACAACG GAGGGGTGCG GCAGGCATAC
    AAGGCTTACC TCAAGTGGAT GGCAGAAGGC GGCAAGGACC AGCAGCTGCC AGGGCTGGGA
    CTGACCTACA ACCAGCTGTT CTTTATCAAC TATGCCCAGG TGTGGTGTGG GTCCTACCGG
    CCTGAGTTTG CCATACAGTC CATCAAGACC GACGTCCATA GCCCCCTGAA GTACAGGGTG
    CTGGGCTCGC TGCAGAACCT GGCAGCCTTC TCGGAGGCCT TCCACTGTGC CCAGGGCAGC
    CCGATGCACC CGCACCAGCG ATGCCGCGTC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288372
    Clone ID Related Accession (Same CDS sequence) XM_022418943.1
    Accession Version XM_022418943.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTCAGA GAGCTCATCG ACAAGGTACG GGCGGTATTC
    GTGGAGACCC TGGATGAGCT GGGCTGGATG GACGAGGAGT CCAAGAAGAA GGCCCAGGAG
    AAGGCCATGA ACATCCGCGA GCAGATTGGG TACCCAGACT ACATTCTGGA GGAAAGAAAC
    AAGCACCTGG ACGAGGAGTA CTCCAATCTG AACTTCTCGG AGGACCAGTA CTTTGAAAAC
    GGTCTCCAGA ACCTCAAGGC TGGCGCCCAG CGGAGCCTCA AGAAGCTGCG GGAGAAAGTA
    GACCAGAACC TCTGGATCAT TGGGGCGGCC GTGGTTAATG CATTCTACTC CCCGAACCGA
    AACCAAATAG TGTTCCCTGC TGGGATCCTA CAGCCTCCAT TCTTCAGCAA ACATCAGCCT
    CAGGCCTTGA ACTTCGGGGG CATTGGGATG GTGATTGGGC ATGAGATCAC CCACGGCTTT
    GACGACAACG GCCGTAACTT TGACAAGAAT GGGAACATGC TGGATTGGTG GAGCAATTTC
    TCAGCCCAGC ACTTCCGGGA GCAGTCGGAG TGCATGGTCC ACCAGTACGG GAACTACTCC
    TGGGACCTGG CGGACAACCA AAACGTGAAC GGGTTCAGCA CACTCGGGGA AAACATCGCC
    GACAACGGAG GGGTGCGGCA GGCATACAAG GCTTACCTCA AGTGGATGGC AGAAGGCGGC
    AAGGACCAGC AGCTGCCAGG GCTGGGACTG ACCTACAACC AGCTGTTCTT TATCAACTAT
    GCCCAGGTGT GGTGTGGGTC CTACCGGCCT GAGTTTGCCA TACAGTCCAT CAAGACCGAC
    GTCCATAGCC CCCTGAAGTA CAGGGTGCTG GGCTCGCTGC AGAACCTGGC AGCCTTCTCG
    GAGGCCTTCC ACTGTGCCCA GGGCAGCCCG ATGCACCCGC ACCAGCGATG CCGCGTCTGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274651.1
    CDS206..2608
    Translation

    Target ORF information:

    RefSeq Version XM_022418943.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418943.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGCGCT GTACGGCACA ACCGTGGAAG AGGTGCGCTG GCGAGAGTGT
    GTTAGCTACG TCAACAGCAA CATGGAGAGC GCTGTGGGCT CCCTCTACGT CAGGGAGGCC
    TTCCCTGGAG ACAGCAAGGA CGCGGTCAGA GAGCTCATCG ACAAGGTACG GGCGGTATTC
    GTGGAGACCC TGGATGAGCT GGGCTGGATG GACGAGGAGT CCAAGAAGAA GGCCCAGGAG
    AAGGCCATGA ACATCCGCGA GCAGATTGGG TACCCAGACT ACATTCTGGA GGAAAGAAAC
    AAGCACCTGG ACGAGGAGTA CTCCAATCTG AACTTCTCGG AGGACCAGTA CTTTGAAAAC
    GGTCTCCAGA ACCTCAAGGC TGGCGCCCAG CGGAGCCTCA AGAAGCTGCG GGAGAAAGTA
    GACCAGAACC TCTGGATCAT TGGGGCGGCC GTGGTTAATG CATTCTACTC CCCGAACCGA
    AACCAAATAG TGTTCCCTGC TGGGATCCTA CAGCCTCCAT TCTTCAGCAA ACATCAGCCT
    CAGGCCTTGA ACTTCGGGGG CATTGGGATG GTGATTGGGC ATGAGATCAC CCACGGCTTT
    GACGACAACG GCCGTAACTT TGACAAGAAT GGGAACATGC TGGATTGGTG GAGCAATTTC
    TCAGCCCAGC ACTTCCGGGA GCAGTCGGAG TGCATGGTCC ACCAGTACGG GAACTACTCC
    TGGGACCTGG CGGACAACCA AAACGTGAAC GGGTTCAGCA CACTCGGGGA AAACATCGCC
    GACAACGGAG GGGTGCGGCA GGCATACAAG GCTTACCTCA AGTGGATGGC AGAAGGCGGC
    AAGGACCAGC AGCTGCCAGG GCTGGGACTG ACCTACAACC AGCTGTTCTT TATCAACTAT
    GCCCAGGTGT GGTGTGGGTC CTACCGGCCT GAGTTTGCCA TACAGTCCAT CAAGACCGAC
    GTCCATAGCC CCCTGAAGTA CAGGGTGCTG GGCTCGCTGC AGAACCTGGC AGCCTTCTCG
    GAGGCCTTCC ACTGTGCCCA GGGCAGCCCG ATGCACCCGC ACCAGCGATG CCGCGTCTGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288373
    Clone ID Related Accession (Same CDS sequence) XM_022418945.1 , XM_022418944.1
    Accession Version XM_022418945.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGGCGAAA TCTGCACCAC CCCTGGCTGT GTGATAGCGG CCTCCAGGAT CCTCCAGAAC 
    ATGGACCCGT CCAGGGAGCC CTGTGAAGAC TTCTACCAGT ACGCATGCGG AGGCTGGCTG
    CGGCGCCACG TGATCCCCGA GACCAACTCC CGATACAGTG TCTTTGACAT CCTCCGAGAC
    GAGCTGGAGG TCATCCTCAA AGCTGCCTCG GGCCTCCATC CAGCTTCCCC TCTGCCCGCA
    GGGGTGCTAG AGAATGCCAC CACCCAGGAC AGGCCGGCTG TGCAGAAGGC CAAGATGCTA
    TACCGCTCCT GCATGAACCA GAGCGTGATA GAGAAACGAG ACTCACAGCC CCTGCTAAAC
    ATCCTGGACG TGATGGGAGG CTGGCCAGTG ACCATGGATA AGTGGAACCA GAGCGTAGCC
    CGAGGCACTG ACTCCCCAGG CCCCAAGTGG GAGCTGGAGC AGCAGCTGGC CTTGATGAAC
    ACACAGTTCA ACAGGCGTGT CCTCATTGAC CTCTTCATCT GGAACGATGA CCAGAATTCT
    AGCCGGCATA TCATCTACAT AGACCAGCCC ACCTTGGGCA TGCCATCCAG AGAGTACTAT
    TTCAACGAGG GCAGCAACCG GAAGGTGCGG GAAGCCTACC TGCAGTTCAT GATGTCGGTG
    GCCATGATGC TGCGGGCAGA CATGAACCTC CCAGAGAACA GCTACCTGGT GCGGGAGGAC
    ATGGTGCAGG TGCTGGAGCT GGAGACGCAG CTGGCAAACG CCACGGCCCC CCAGGAGGAG
    AGGCACGATG TCACTGCCCT ATATCATAGG ATGGGCCTGA AGGAACTCCA GAGCAAGTTT
    AGCCTGAAGG GGTTTAACTG GACCCTCTTC ATACAATCTG TGCTGTCCTC TGTCAAAATC
    AAACTGTTGC CAGATGAAGA AGTGGTGGTC TATGGCATCC CCTACCTTCA GAACCTTGAA
    GAAATCATCG ATGTCTACTC AGCCAGGACC ATGCAGAACT ACCTGGTCTG GCGCCTGGTG
    CTGGACCGCA TTAGTAGCCT GAGCCAGCGA TTCAAGGATG CACGGGCAAA CTACCGCAAG
    GCGCTGTACG GCACAACCGT GGAAGAGGTG CGCTGGCGAG AGTGTGTTAG CTACGTCAAC
    AGCAACATGG AGAGCGCTGT GGGCTCCCTC TACGTCAGGG AGGCCTTCCC TGGAGACAGC
    AAGGACGCGG TGGGTGCTCG GCACTGGGAC TGCTCCGCAC CCCTGCCCAT GGTCCATTGC
    CCAGGGCCAG CAGGGACGGG GCTTTGGACC AGACCCCTGC AGCGACCCCG GAGCTGGTCA
    TGCAGGACAA GGGCCATGCA CTCCCCCCAG GTCAGAGAGC TCATCGACAA GGTACGGGCG
    GTATTCGTGG AGACCCTGGA TGAGCTGGGC TGGATGGACG AGGAGTCCAA GAAGAAGGCC
    CAGGAGAAGG CCATGAACAT CCGCGAGCAG ATTGGGTACC CAGACTACAT TCTGGAGGAA
    AGAAACAAGC ACCTGGACGA GGAGTACTCC AATCTGAACT TCTCGGAGGA CCAGTACTTT
    GAAAACGGTC TCCAGAACCT CAAGGCTGGC GCCCAGCGGA GCCTCAAGAA GCTGCGGGAG
    AAAGTAGACC AGAACCTGGG CCCCAAGCAG GGGAGCCGCA TTCAGCCAGC AGGGGGCCAG
    GACGGAGGAC TCCCAGGCAC GGGCTTGGGT GACCTGCCCG TTCATCACAG CTGGATCATT
    GGGGCGGCCG TGGTTAATGC ATTCTACTCC CCGAACCGAA ACCAAATAGT GTTCCCTGCT
    GGGATCCTAC AGCCTCCATT CTTCAGCAAA CATCAGCCTC AGGCCTTGAA CTTCGGGGGC
    ATTGGGATGG TGATTGGGCA TGAGATCACC CACGGCTTTG ACGACAACGG CCGTAACTTT
    GACAAGAATG GGAACATGCT GGATTGGTGG AGCAATTTCT CAGCCCAGCA CTTCCGGGAG
    CAGTCGGAGT GCATGGTCCA CCAGTACGGG AACTACTCCT GGGACCTGGC GGACAACCAA
    AACGTGAACG GGTTCAGCAC ACTCGGGGAA AACATCGCCG ACAACGGAGG GGTGCGGCAG
    GCATACAAGG CTTACCTCAA GTGGATGGCA GAAGGCGGCA AGGACCAGCA GCTGCCAGGG
    CTGGGACTGA CCTACAACCA GCTGTTCTTT ATCAACTATG CCCAGGTGTG GTGTGGGTCC
    TACCGGCCTG AGTTTGCCAT ACAGTCCATC AAGACCGACG TCCATAGCCC CCTGAAGTAC
    AGGGTGCTGG GCTCGCTGCA GAACCTGGCA GCCTTCTCGG AGGCCTTCCA CTGTGCCCAG
    GGCAGCCCGA TGCACCCGCA CCAGCGATGC CGCGTCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274653.1
    CDS355..2736
    Translation

    Target ORF information:

    RefSeq Version XM_022418945.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418945.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGGCGAAA TCTGCACCAC CCCTGGCTGT GTGATAGCGG CCTCCAGGAT CCTCCAGAAC 
    ATGGACCCGT CCAGGGAGCC CTGTGAAGAC TTCTACCAGT ACGCATGCGG AGGCTGGCTG
    CGGCGCCACG TGATCCCCGA GACCAACTCC CGATACAGTG TCTTTGACAT CCTCCGAGAC
    GAGCTGGAGG TCATCCTCAA AGCTGCCTCG GGCCTCCATC CAGCTTCCCC TCTGCCCGCA
    GGGGTGCTAG AGAATGCCAC CACCCAGGAC AGGCCGGCTG TGCAGAAGGC CAAGATGCTA
    TACCGCTCCT GCATGAACCA GAGCGTGATA GAGAAACGAG ACTCACAGCC CCTGCTAAAC
    ATCCTGGACG TGATGGGAGG CTGGCCAGTG ACCATGGATA AGTGGAACCA GAGCGTAGCC
    CGAGGCACTG ACTCCCCAGG CCCCAAGTGG GAGCTGGAGC AGCAGCTGGC CTTGATGAAC
    ACACAGTTCA ACAGGCGTGT CCTCATTGAC CTCTTCATCT GGAACGATGA CCAGAATTCT
    AGCCGGCATA TCATCTACAT AGACCAGCCC ACCTTGGGCA TGCCATCCAG AGAGTACTAT
    TTCAACGAGG GCAGCAACCG GAAGGTGCGG GAAGCCTACC TGCAGTTCAT GATGTCGGTG
    GCCATGATGC TGCGGGCAGA CATGAACCTC CCAGAGAACA GCTACCTGGT GCGGGAGGAC
    ATGGTGCAGG TGCTGGAGCT GGAGACGCAG CTGGCAAACG CCACGGCCCC CCAGGAGGAG
    AGGCACGATG TCACTGCCCT ATATCATAGG ATGGGCCTGA AGGAACTCCA GAGCAAGTTT
    AGCCTGAAGG GGTTTAACTG GACCCTCTTC ATACAATCTG TGCTGTCCTC TGTCAAAATC
    AAACTGTTGC CAGATGAAGA AGTGGTGGTC TATGGCATCC CCTACCTTCA GAACCTTGAA
    GAAATCATCG ATGTCTACTC AGCCAGGACC ATGCAGAACT ACCTGGTCTG GCGCCTGGTG
    CTGGACCGCA TTAGTAGCCT GAGCCAGCGA TTCAAGGATG CACGGGCAAA CTACCGCAAG
    GCGCTGTACG GCACAACCGT GGAAGAGGTG CGCTGGCGAG AGTGTGTTAG CTACGTCAAC
    AGCAACATGG AGAGCGCTGT GGGCTCCCTC TACGTCAGGG AGGCCTTCCC TGGAGACAGC
    AAGGACGCGG TGGGTGCTCG GCACTGGGAC TGCTCCGCAC CCCTGCCCAT GGTCCATTGC
    CCAGGGCCAG CAGGGACGGG GCTTTGGACC AGACCCCTGC AGCGACCCCG GAGCTGGTCA
    TGCAGGACAA GGGCCATGCA CTCCCCCCAG GTCAGAGAGC TCATCGACAA GGTACGGGCG
    GTATTCGTGG AGACCCTGGA TGAGCTGGGC TGGATGGACG AGGAGTCCAA GAAGAAGGCC
    CAGGAGAAGG CCATGAACAT CCGCGAGCAG ATTGGGTACC CAGACTACAT TCTGGAGGAA
    AGAAACAAGC ACCTGGACGA GGAGTACTCC AATCTGAACT TCTCGGAGGA CCAGTACTTT
    GAAAACGGTC TCCAGAACCT CAAGGCTGGC GCCCAGCGGA GCCTCAAGAA GCTGCGGGAG
    AAAGTAGACC AGAACCTGGG CCCCAAGCAG GGGAGCCGCA TTCAGCCAGC AGGGGGCCAG
    GACGGAGGAC TCCCAGGCAC GGGCTTGGGT GACCTGCCCG TTCATCACAG CTGGATCATT
    GGGGCGGCCG TGGTTAATGC ATTCTACTCC CCGAACCGAA ACCAAATAGT GTTCCCTGCT
    GGGATCCTAC AGCCTCCATT CTTCAGCAAA CATCAGCCTC AGGCCTTGAA CTTCGGGGGC
    ATTGGGATGG TGATTGGGCA TGAGATCACC CACGGCTTTG ACGACAACGG CCGTAACTTT
    GACAAGAATG GGAACATGCT GGATTGGTGG AGCAATTTCT CAGCCCAGCA CTTCCGGGAG
    CAGTCGGAGT GCATGGTCCA CCAGTACGGG AACTACTCCT GGGACCTGGC GGACAACCAA
    AACGTGAACG GGTTCAGCAC ACTCGGGGAA AACATCGCCG ACAACGGAGG GGTGCGGCAG
    GCATACAAGG CTTACCTCAA GTGGATGGCA GAAGGCGGCA AGGACCAGCA GCTGCCAGGG
    CTGGGACTGA CCTACAACCA GCTGTTCTTT ATCAACTATG CCCAGGTGTG GTGTGGGTCC
    TACCGGCCTG AGTTTGCCAT ACAGTCCATC AAGACCGACG TCCATAGCCC CCTGAAGTAC
    AGGGTGCTGG GCTCGCTGCA GAACCTGGCA GCCTTCTCGG AGGCCTTCCA CTGTGCCCAG
    GGCAGCCCGA TGCACCCGCA CCAGCGATGC CGCGTCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288373
    Clone ID Related Accession (Same CDS sequence) XM_022418945.1 , XM_022418944.1
    Accession Version XM_022418944.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGGCGAAA TCTGCACCAC CCCTGGCTGT GTGATAGCGG CCTCCAGGAT CCTCCAGAAC 
    ATGGACCCGT CCAGGGAGCC CTGTGAAGAC TTCTACCAGT ACGCATGCGG AGGCTGGCTG
    CGGCGCCACG TGATCCCCGA GACCAACTCC CGATACAGTG TCTTTGACAT CCTCCGAGAC
    GAGCTGGAGG TCATCCTCAA AGCTGCCTCG GGCCTCCATC CAGCTTCCCC TCTGCCCGCA
    GGGGTGCTAG AGAATGCCAC CACCCAGGAC AGGCCGGCTG TGCAGAAGGC CAAGATGCTA
    TACCGCTCCT GCATGAACCA GAGCGTGATA GAGAAACGAG ACTCACAGCC CCTGCTAAAC
    ATCCTGGACG TGATGGGAGG CTGGCCAGTG ACCATGGATA AGTGGAACCA GAGCGTAGCC
    CGAGGCACTG ACTCCCCAGG CCCCAAGTGG GAGCTGGAGC AGCAGCTGGC CTTGATGAAC
    ACACAGTTCA ACAGGCGTGT CCTCATTGAC CTCTTCATCT GGAACGATGA CCAGAATTCT
    AGCCGGCATA TCATCTACAT AGACCAGCCC ACCTTGGGCA TGCCATCCAG AGAGTACTAT
    TTCAACGAGG GCAGCAACCG GAAGGTGCGG GAAGCCTACC TGCAGTTCAT GATGTCGGTG
    GCCATGATGC TGCGGGCAGA CATGAACCTC CCAGAGAACA GCTACCTGGT GCGGGAGGAC
    ATGGTGCAGG TGCTGGAGCT GGAGACGCAG CTGGCAAACG CCACGGCCCC CCAGGAGGAG
    AGGCACGATG TCACTGCCCT ATATCATAGG ATGGGCCTGA AGGAACTCCA GAGCAAGTTT
    AGCCTGAAGG GGTTTAACTG GACCCTCTTC ATACAATCTG TGCTGTCCTC TGTCAAAATC
    AAACTGTTGC CAGATGAAGA AGTGGTGGTC TATGGCATCC CCTACCTTCA GAACCTTGAA
    GAAATCATCG ATGTCTACTC AGCCAGGACC ATGCAGAACT ACCTGGTCTG GCGCCTGGTG
    CTGGACCGCA TTAGTAGCCT GAGCCAGCGA TTCAAGGATG CACGGGCAAA CTACCGCAAG
    GCGCTGTACG GCACAACCGT GGAAGAGGTG CGCTGGCGAG AGTGTGTTAG CTACGTCAAC
    AGCAACATGG AGAGCGCTGT GGGCTCCCTC TACGTCAGGG AGGCCTTCCC TGGAGACAGC
    AAGGACGCGG TGGGTGCTCG GCACTGGGAC TGCTCCGCAC CCCTGCCCAT GGTCCATTGC
    CCAGGGCCAG CAGGGACGGG GCTTTGGACC AGACCCCTGC AGCGACCCCG GAGCTGGTCA
    TGCAGGACAA GGGCCATGCA CTCCCCCCAG GTCAGAGAGC TCATCGACAA GGTACGGGCG
    GTATTCGTGG AGACCCTGGA TGAGCTGGGC TGGATGGACG AGGAGTCCAA GAAGAAGGCC
    CAGGAGAAGG CCATGAACAT CCGCGAGCAG ATTGGGTACC CAGACTACAT TCTGGAGGAA
    AGAAACAAGC ACCTGGACGA GGAGTACTCC AATCTGAACT TCTCGGAGGA CCAGTACTTT
    GAAAACGGTC TCCAGAACCT CAAGGCTGGC GCCCAGCGGA GCCTCAAGAA GCTGCGGGAG
    AAAGTAGACC AGAACCTGGG CCCCAAGCAG GGGAGCCGCA TTCAGCCAGC AGGGGGCCAG
    GACGGAGGAC TCCCAGGCAC GGGCTTGGGT GACCTGCCCG TTCATCACAG CTGGATCATT
    GGGGCGGCCG TGGTTAATGC ATTCTACTCC CCGAACCGAA ACCAAATAGT GTTCCCTGCT
    GGGATCCTAC AGCCTCCATT CTTCAGCAAA CATCAGCCTC AGGCCTTGAA CTTCGGGGGC
    ATTGGGATGG TGATTGGGCA TGAGATCACC CACGGCTTTG ACGACAACGG CCGTAACTTT
    GACAAGAATG GGAACATGCT GGATTGGTGG AGCAATTTCT CAGCCCAGCA CTTCCGGGAG
    CAGTCGGAGT GCATGGTCCA CCAGTACGGG AACTACTCCT GGGACCTGGC GGACAACCAA
    AACGTGAACG GGTTCAGCAC ACTCGGGGAA AACATCGCCG ACAACGGAGG GGTGCGGCAG
    GCATACAAGG CTTACCTCAA GTGGATGGCA GAAGGCGGCA AGGACCAGCA GCTGCCAGGG
    CTGGGACTGA CCTACAACCA GCTGTTCTTT ATCAACTATG CCCAGGTGTG GTGTGGGTCC
    TACCGGCCTG AGTTTGCCAT ACAGTCCATC AAGACCGACG TCCATAGCCC CCTGAAGTAC
    AGGGTGCTGG GCTCGCTGCA GAACCTGGCA GCCTTCTCGG AGGCCTTCCA CTGTGCCCAG
    GGCAGCCCGA TGCACCCGCA CCAGCGATGC CGCGTCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274652.1
    CDS328..2709
    Translation

    Target ORF information:

    RefSeq Version XM_022418944.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418944.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGGCGAAA TCTGCACCAC CCCTGGCTGT GTGATAGCGG CCTCCAGGAT CCTCCAGAAC 
    ATGGACCCGT CCAGGGAGCC CTGTGAAGAC TTCTACCAGT ACGCATGCGG AGGCTGGCTG
    CGGCGCCACG TGATCCCCGA GACCAACTCC CGATACAGTG TCTTTGACAT CCTCCGAGAC
    GAGCTGGAGG TCATCCTCAA AGCTGCCTCG GGCCTCCATC CAGCTTCCCC TCTGCCCGCA
    GGGGTGCTAG AGAATGCCAC CACCCAGGAC AGGCCGGCTG TGCAGAAGGC CAAGATGCTA
    TACCGCTCCT GCATGAACCA GAGCGTGATA GAGAAACGAG ACTCACAGCC CCTGCTAAAC
    ATCCTGGACG TGATGGGAGG CTGGCCAGTG ACCATGGATA AGTGGAACCA GAGCGTAGCC
    CGAGGCACTG ACTCCCCAGG CCCCAAGTGG GAGCTGGAGC AGCAGCTGGC CTTGATGAAC
    ACACAGTTCA ACAGGCGTGT CCTCATTGAC CTCTTCATCT GGAACGATGA CCAGAATTCT
    AGCCGGCATA TCATCTACAT AGACCAGCCC ACCTTGGGCA TGCCATCCAG AGAGTACTAT
    TTCAACGAGG GCAGCAACCG GAAGGTGCGG GAAGCCTACC TGCAGTTCAT GATGTCGGTG
    GCCATGATGC TGCGGGCAGA CATGAACCTC CCAGAGAACA GCTACCTGGT GCGGGAGGAC
    ATGGTGCAGG TGCTGGAGCT GGAGACGCAG CTGGCAAACG CCACGGCCCC CCAGGAGGAG
    AGGCACGATG TCACTGCCCT ATATCATAGG ATGGGCCTGA AGGAACTCCA GAGCAAGTTT
    AGCCTGAAGG GGTTTAACTG GACCCTCTTC ATACAATCTG TGCTGTCCTC TGTCAAAATC
    AAACTGTTGC CAGATGAAGA AGTGGTGGTC TATGGCATCC CCTACCTTCA GAACCTTGAA
    GAAATCATCG ATGTCTACTC AGCCAGGACC ATGCAGAACT ACCTGGTCTG GCGCCTGGTG
    CTGGACCGCA TTAGTAGCCT GAGCCAGCGA TTCAAGGATG CACGGGCAAA CTACCGCAAG
    GCGCTGTACG GCACAACCGT GGAAGAGGTG CGCTGGCGAG AGTGTGTTAG CTACGTCAAC
    AGCAACATGG AGAGCGCTGT GGGCTCCCTC TACGTCAGGG AGGCCTTCCC TGGAGACAGC
    AAGGACGCGG TGGGTGCTCG GCACTGGGAC TGCTCCGCAC CCCTGCCCAT GGTCCATTGC
    CCAGGGCCAG CAGGGACGGG GCTTTGGACC AGACCCCTGC AGCGACCCCG GAGCTGGTCA
    TGCAGGACAA GGGCCATGCA CTCCCCCCAG GTCAGAGAGC TCATCGACAA GGTACGGGCG
    GTATTCGTGG AGACCCTGGA TGAGCTGGGC TGGATGGACG AGGAGTCCAA GAAGAAGGCC
    CAGGAGAAGG CCATGAACAT CCGCGAGCAG ATTGGGTACC CAGACTACAT TCTGGAGGAA
    AGAAACAAGC ACCTGGACGA GGAGTACTCC AATCTGAACT TCTCGGAGGA CCAGTACTTT
    GAAAACGGTC TCCAGAACCT CAAGGCTGGC GCCCAGCGGA GCCTCAAGAA GCTGCGGGAG
    AAAGTAGACC AGAACCTGGG CCCCAAGCAG GGGAGCCGCA TTCAGCCAGC AGGGGGCCAG
    GACGGAGGAC TCCCAGGCAC GGGCTTGGGT GACCTGCCCG TTCATCACAG CTGGATCATT
    GGGGCGGCCG TGGTTAATGC ATTCTACTCC CCGAACCGAA ACCAAATAGT GTTCCCTGCT
    GGGATCCTAC AGCCTCCATT CTTCAGCAAA CATCAGCCTC AGGCCTTGAA CTTCGGGGGC
    ATTGGGATGG TGATTGGGCA TGAGATCACC CACGGCTTTG ACGACAACGG CCGTAACTTT
    GACAAGAATG GGAACATGCT GGATTGGTGG AGCAATTTCT CAGCCCAGCA CTTCCGGGAG
    CAGTCGGAGT GCATGGTCCA CCAGTACGGG AACTACTCCT GGGACCTGGC GGACAACCAA
    AACGTGAACG GGTTCAGCAC ACTCGGGGAA AACATCGCCG ACAACGGAGG GGTGCGGCAG
    GCATACAAGG CTTACCTCAA GTGGATGGCA GAAGGCGGCA AGGACCAGCA GCTGCCAGGG
    CTGGGACTGA CCTACAACCA GCTGTTCTTT ATCAACTATG CCCAGGTGTG GTGTGGGTCC
    TACCGGCCTG AGTTTGCCAT ACAGTCCATC AAGACCGACG TCCATAGCCC CCTGAAGTAC
    AGGGTGCTGG GCTCGCTGCA GAACCTGGCA GCCTTCTCGG AGGCCTTCCA CTGTGCCCAG
    GGCAGCCCGA TGCACCCGCA CCAGCGATGC CGCGTCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288374
    Clone ID Related Accession (Same CDS sequence) XM_022418946.1
    Accession Version XM_022418946.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product membrane metallo-endopeptidase-like 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGTCAG AGAGCTCATC GACAAGGTAC GGGCGGTATT CGTGGAGACC
    CTGGATGAGC TGGGCTGGAT GGACGAGGAG TCCAAGAAGA AGGCCCAGGA GAAGGCCATG
    AACATCCGCG AGCAGATTGG GTACCCAGAC TACATTCTGG AGGAAAGAAA CAAGCACCTG
    GACGAGGAGT ACTCCAATCT GAACTTCTCG GAGGACCAGT ACTTTGAAAA CGGTCTCCAG
    AACCTCAAGG CTGGCGCCCA GCGGAGCCTC AAGAAGCTGC GGGAGAAAGT AGACCAGAAC
    CTGGGCCCCA AGCAGGGGAG CCGCATTCAG CCAGCAGGGG GCCAGGACGG AGGACTCCCA
    GGCACGGGCT TGGGTGACCT GCCCGTTCAT CACAGCTGGA TCATTGGGGC GGCCGTGGTT
    AATGCATTCT ACTCCCCGAA CCGAAACCAA ATAGTGTTCC CTGCTGGGAT CCTACAGCCT
    CCATTCTTCA GCAAACATCA GCCTCAGGCC TTGAACTTCG GGGGCATTGG GATGGTGATT
    GGGCATGAGA TCACCCACGG CTTTGACGAC AACGGCCGTA ACTTTGACAA GAATGGGAAC
    ATGCTGGATT GGTGGAGCAA TTTCTCAGCC CAGCACTTCC GGGAGCAGTC GGAGTGCATG
    GTCCACCAGT ACGGGAACTA CTCCTGGGAC CTGGCGGACA ACCAAAACGT GAACGGGTTC
    AGCACACTCG GGGAAAACAT CGCCGACAAC GGAGGGGTGC GGCAGGCATA CAAGGCTTAC
    CTCAAGTGGA TGGCAGAAGG CGGCAAGGAC CAGCAGCTGC CAGGGCTGGG ACTGACCTAC
    AACCAGCTGT TCTTTATCAA CTATGCCCAG GTGTGGTGTG GGTCCTACCG GCCTGAGTTT
    GCCATACAGT CCATCAAGAC CGACGTCCAT AGCCCCCTGA AGTACAGGGT GCTGGGCTCG
    CTGCAGAACC TGGCAGCCTT CTCGGAGGCC TTCCACTGTG CCCAGGGCAG CCCGATGCAC
    CCGCACCAGC GATGCCGCGT CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274654.1
    CDS221..2587
    Translation

    Target ORF information:

    RefSeq Version XM_022418946.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane metalloendopeptidase like 1 (MMEL1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418946.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGGGAAGC CCGAAAGCCC GGTAGGGATG GTGGAGATTG CTGGCTGGCC GGGACAGAAG 
    CACCGAGGCT TCCTGGAGGG GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTGGCCCTGG GCCTCCTCTA TGCTGCCAAC AGCAGAGGGA AGCAGCTGAC ACTCTTTCCT
    AGCCGGCTTT GCTTCTCAAA GGAAGAGAAG ACCATTGTAA AACGAAAACC CCGAGCCATC
    CAAGAGCCCA AGGAGATGGG CGAAATCTGC ACCACCCCTG GCTGTGTGAT AGCGGCCTCC
    AGGATCCTCC AGAACATGGA CCCGTCCAGG GAGCCCTGTG AAGACTTCTA CCAGTACGCA
    TGCGGAGGCT GGCTGCGGCG CCACGTGATC CCCGAGACCA ACTCCCGATA CAGTGTCTTT
    GACATCCTCC GAGACGAGCT GGAGGTCATC CTCAAAGCTG CCTCGGGCCT CCATCCAGCT
    TCCCCTCTGC CCGCAGGGGT GCTAGAGAAT GCCACCACCC AGGACAGGCC GGCTGTGCAG
    AAGGCCAAGA TGCTATACCG CTCCTGCATG AACCAGAGCG TGATAGAGAA ACGAGACTCA
    CAGCCCCTGC TAAACATCCT GGACGTGATG GGAGGCTGGC CAGTGACCAT GGATAAGTGG
    AACCAGAGCG TAGCCCGAGG CACTGACTCC CCAGGCCCCA AGTGGGAGCT GGAGCAGCAG
    CTGGCCTTGA TGAACACACA GTTCAACAGG CGTGTCCTCA TTGACCTCTT CATCTGGAAC
    GATGACCAGA ATTCTAGCCG GCATATCATC TACATAGACC AGCCCACCTT GGGCATGCCA
    TCCAGAGAGT ACTATTTCAA CGAGGGCAGC AACCGGAAGG TGCGGGAAGC CTACCTGCAG
    TTCATGATGT CGGTGGCCAT GATGCTGCGG GCAGACATGA ACCTCCCAGA GAACAGCTAC
    CTGGTGCGGG AGGACATGGT GCAGGTGCTG GAGCTGGAGA CGCAGCTGGC AAACGCCACG
    GCCCCCCAGG AGGAGAGGCA CGATGTCACT GCCCTATATC ATAGGATGGG CCTGAAGGAA
    CTCCAGAGCA AGTTTAGCCT GAAGGGGTTT AACTGGACCC TCTTCATACA ATCTGTGCTG
    TCCTCTGTCA AAATCAAACT GTTGCCAGAT GAAGAAGTGG TGGTCTATGG CATCCCCTAC
    CTTCAGAACC TTGAAGAAAT CATCGATGTC TACTCAGCCA GGACCATGCA GAACTACCTG
    GTCTGGCGCC TGGTGCTGGA CCGCATTAGT AGCCTGAGCC AGCGATTCAA GGATGCACGG
    GCAAACTACC GCAAGGTCAG AGAGCTCATC GACAAGGTAC GGGCGGTATT CGTGGAGACC
    CTGGATGAGC TGGGCTGGAT GGACGAGGAG TCCAAGAAGA AGGCCCAGGA GAAGGCCATG
    AACATCCGCG AGCAGATTGG GTACCCAGAC TACATTCTGG AGGAAAGAAA CAAGCACCTG
    GACGAGGAGT ACTCCAATCT GAACTTCTCG GAGGACCAGT ACTTTGAAAA CGGTCTCCAG
    AACCTCAAGG CTGGCGCCCA GCGGAGCCTC AAGAAGCTGC GGGAGAAAGT AGACCAGAAC
    CTGGGCCCCA AGCAGGGGAG CCGCATTCAG CCAGCAGGGG GCCAGGACGG AGGACTCCCA
    GGCACGGGCT TGGGTGACCT GCCCGTTCAT CACAGCTGGA TCATTGGGGC GGCCGTGGTT
    AATGCATTCT ACTCCCCGAA CCGAAACCAA ATAGTGTTCC CTGCTGGGAT CCTACAGCCT
    CCATTCTTCA GCAAACATCA GCCTCAGGCC TTGAACTTCG GGGGCATTGG GATGGTGATT
    GGGCATGAGA TCACCCACGG CTTTGACGAC AACGGCCGTA ACTTTGACAA GAATGGGAAC
    ATGCTGGATT GGTGGAGCAA TTTCTCAGCC CAGCACTTCC GGGAGCAGTC GGAGTGCATG
    GTCCACCAGT ACGGGAACTA CTCCTGGGAC CTGGCGGACA ACCAAAACGT GAACGGGTTC
    AGCACACTCG GGGAAAACAT CGCCGACAAC GGAGGGGTGC GGCAGGCATA CAAGGCTTAC
    CTCAAGTGGA TGGCAGAAGG CGGCAAGGAC CAGCAGCTGC CAGGGCTGGG ACTGACCTAC
    AACCAGCTGT TCTTTATCAA CTATGCCCAG GTGTGGTGTG GGTCCTACCG GCCTGAGTTT
    GCCATACAGT CCATCAAGAC CGACGTCCAT AGCCCCCTGA AGTACAGGGT GCTGGGCTCG
    CTGCAGAACC TGGCAGCCTT CTCGGAGGCC TTCCACTGTG CCCAGGGCAG CCCGATGCAC
    CCGCACCAGC GATGCCGCGT CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.