MMEL1 cDNA ORF clone, Bos taurus(cattle)

The following MMEL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMEL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa36279 NM_001193136.1
Latest version!
Bos taurus membrane metalloendopeptidase like 1 (MMEL1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa223758 XM_024976520.1
Latest version!
Bos taurus membrane metalloendopeptidase like 1 (MMEL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa27331 XM_024976519.1
Latest version!
Bos taurus membrane metalloendopeptidase like 1 (MMEL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa56190 XM_024976521.1
Latest version!
Bos taurus membrane metalloendopeptidase like 1 (MMEL1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa92975 XM_015475156.1
Latest version!
Bos taurus membrane metallo-endopeptidase-like 1 (MMEL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa36279
    Clone ID Related Accession (Same CDS sequence) NM_001193136.1
    Accession Version NM_001193136.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product membrane metallo-endopeptidase-like 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000016.1. On Jul 17, 2010 this sequence version replaced XM_869160.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAAGC CCGAGAGCCC AGTGGGGATG GTGGAGATCG CCGGCCGCTC GGGGCAGAAG 
    CACCGAGGCT TCCTGGAGGC GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTCACCCTGG GCCTCCTCTA CGCCGACAGC AGAGGGAAGC GGCTGCCACT CTTTTCTAGC
    CGGCTTTGCT TCTCCAAGGA AGAGAAGAAC ACTGTAAAAC GAGCACCCCG AGAGATCAAA
    CCCTCCAAGG ACAATGTCGA GACCTGCACC ACCCCTGGCT GCGTGATGGC AGCTGCCAGG
    ATCCTCCAGA ACATGGACCC ATCCATGGAG CCGTGTAACG ACTTCTACCA GTACGCATGC
    GGGGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CGCGCTACAG CGTCTTCGAC
    ATCCTCCGGG ATGAGCTGGA GATCATCCTC AAAGCGGTGC TGGAGAACTC CACCACCAAA
    GACCGGCCGG CCGTGCAAAA GGCCAAGATG CTCTACCGCT CCTGCATGAA CGAAAGTGAG
    ATAGAGAAGC GAGACTCACA GCCCCTGCTG AATATCCTGG AGGTGGTGGG AGGCTGGCCA
    GTGGCCATGG ACAAGTGGAA CAAGAGCGTA GGCCCCCAGT GGGAGCTGGA ACAGCAGCTG
    GCCGTGATGA ACGCACAGTT CAACCGGCGG GTGCTCATCG ACCTCTTCAT CTGGAACGAC
    GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAAC CCACCCTGGG CATGCCGTCC
    CGAGAGTACT ACTTCAAGGA GGGCAACAAC CAGAAGGTAC GGGAAGCCTA CCTGCAGTTC
    ATGGTGTCGG TGGCCATGAT GCTGCGGGCG GACTTGAACC TCCCCGAGAA CAGCGGCCTG
    GTTCAGGAAG ACATGGCCCA GGTGCTGGAG CTGGAGACGC AGCTGGCCAA CGCCACGGTC
    CCCCAGGAAG AGCGCCATGA CGTCACCGCC CTGTACCACA GGATGGACCT GGAGCAGCTC
    CAGAACAGCT TCGGTCTGAA GGGATTTAAC TGGACTCTCT TCATACAATC CGTGCTATCC
    TCTGTCAAAA TTGATCTGCT GCCTAATGAA GAAGTGGTGG TCTATGGCAT CCCCTACCTC
    CAGAATCTTG AAGGCATCAT CGATGTCTAC TCACCCAGGA CCATGCAGAA CTACCTGATT
    TGGCGCCTGG TGCTGGACCG CATCAGCAGC CTGAGCCAGC GGTTTAAGGA AGCGCGTGCA
    AGCTACCGCA AGGCACTCTA TGGCACGACG GTGGAGGAGG TTCGCTGGCG GGAGTGCGTC
    AGCTATGTCA ACAGCAACAT GGAGAGCGCC GTGGGCTCGC TCTATGTCAA GGAGGCCTTC
    TCCGGTGACA GCAAGGACGT GGTCAAAGAG CTCATTGACA AGGTGCGGGC AGTGTTCGTG
    GACACTCTGG ATGAGCTGAG CTGGATGGAT GAGTCATCCA AGAAGAAGGC CCAGGAGAAG
    GCCATGAACA TCCGGGAGCA GATTGGGTAC CCTGACTACA TCCTGGAGGA GGGGAACAAA
    CATCTGGACG AGGAGTATTC CAACCTGAAC TTCTCAGAGC ACCTGTACTT TGAGAACGGA
    CTGCAGAATC TGAAGGCTGG CGCCCAGCGG AGCCTCAAGA AGCTTAGGGA GAAGGTGGAC
    CAGAACCTCT GGATCATTGG GGCTGCCGTG GTGAACGCCT TCTACTCTCC AAACCGAAAC
    CAGATTGTGT TCCCCGCTGG GATCCTCCAG CCCCCTTTTT TCAGCAAGGA GCAGCCTCAA
    GCCTTGAACT TCGGGGGCAT CGGGATGGTC ATCGGGCACG AGATCACCCA CGGCTTTGAC
    GACAATGGCC GGAACTTCGA CAAGAATGGG AACATGCTGG ACTGGTGGAG CAACTTCTCT
    GCCCAGCACT TCAGGGAGCA GTCAGAGTGC ATGGTCTACC AATATGGCAA TTACTCCTGG
    GACCTGGCTG ATGACCAGAA TGTGAATGGC TTCAGCACAC TCGGGGAAAA CATTGCCGAC
    AATGGAGGGG TGCGGCAGGC ATACAAGGCT TACCTGAAGT GGGTGGCAGA AGGCGGCAAG
    GACCAGCAGC TGCCAGGCCT GGAACTGACC TACCAGCAAC TGTTCTTCAT CAACTATGCG
    CAGGTGTGGT GCGGATCCTA CCGGCCAGAG TTCGCCATCC AGTCCATCAA GACTGACGTC
    CACAGTCCCC TGAAGTACAG GGTGCTGGGC TCGCTGCAGA ACCTGGGCGC CTTCGCAGAC
    GCGTTCCACT GCGCGAATGG CTCCCCCATG CACCCAACGG TGCGATGCCG CGTCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180065.1
    CDS1..2340
    Translation

    Target ORF information:

    RefSeq Version NM_001193136.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metalloendopeptidase like 1 (MMEL1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193136.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAAGC CCGAGAGCCC AGTGGGGATG GTGGAGATCG CCGGCCGCTC GGGGCAGAAG 
    CACCGAGGCT TCCTGGAGGC GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTCACCCTGG GCCTCCTCTA CGCCGACAGC AGAGGGAAGC GGCTGCCACT CTTTTCTAGC
    CGGCTTTGCT TCTCCAAGGA AGAGAAGAAC ACTGTAAAAC GAGCACCCCG AGAGATCAAA
    CCCTCCAAGG ACAATGTCGA GACCTGCACC ACCCCTGGCT GCGTGATGGC AGCTGCCAGG
    ATCCTCCAGA ACATGGACCC ATCCATGGAG CCGTGTAACG ACTTCTACCA GTACGCATGC
    GGGGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CGCGCTACAG CGTCTTCGAC
    ATCCTCCGGG ATGAGCTGGA GATCATCCTC AAAGCGGTGC TGGAGAACTC CACCACCAAA
    GACCGGCCGG CCGTGCAAAA GGCCAAGATG CTCTACCGCT CCTGCATGAA CGAAAGTGAG
    ATAGAGAAGC GAGACTCACA GCCCCTGCTG AATATCCTGG AGGTGGTGGG AGGCTGGCCA
    GTGGCCATGG ACAAGTGGAA CAAGAGCGTA GGCCCCCAGT GGGAGCTGGA ACAGCAGCTG
    GCCGTGATGA ACGCACAGTT CAACCGGCGG GTGCTCATCG ACCTCTTCAT CTGGAACGAC
    GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAAC CCACCCTGGG CATGCCGTCC
    CGAGAGTACT ACTTCAAGGA GGGCAACAAC CAGAAGGTAC GGGAAGCCTA CCTGCAGTTC
    ATGGTGTCGG TGGCCATGAT GCTGCGGGCG GACTTGAACC TCCCCGAGAA CAGCGGCCTG
    GTTCAGGAAG ACATGGCCCA GGTGCTGGAG CTGGAGACGC AGCTGGCCAA CGCCACGGTC
    CCCCAGGAAG AGCGCCATGA CGTCACCGCC CTGTACCACA GGATGGACCT GGAGCAGCTC
    CAGAACAGCT TCGGTCTGAA GGGATTTAAC TGGACTCTCT TCATACAATC CGTGCTATCC
    TCTGTCAAAA TTGATCTGCT GCCTAATGAA GAAGTGGTGG TCTATGGCAT CCCCTACCTC
    CAGAATCTTG AAGGCATCAT CGATGTCTAC TCACCCAGGA CCATGCAGAA CTACCTGATT
    TGGCGCCTGG TGCTGGACCG CATCAGCAGC CTGAGCCAGC GGTTTAAGGA AGCGCGTGCA
    AGCTACCGCA AGGCACTCTA TGGCACGACG GTGGAGGAGG TTCGCTGGCG GGAGTGCGTC
    AGCTATGTCA ACAGCAACAT GGAGAGCGCC GTGGGCTCGC TCTATGTCAA GGAGGCCTTC
    TCCGGTGACA GCAAGGACGT GGTCAAAGAG CTCATTGACA AGGTGCGGGC AGTGTTCGTG
    GACACTCTGG ATGAGCTGAG CTGGATGGAT GAGTCATCCA AGAAGAAGGC CCAGGAGAAG
    GCCATGAACA TCCGGGAGCA GATTGGGTAC CCTGACTACA TCCTGGAGGA GGGGAACAAA
    CATCTGGACG AGGAGTATTC CAACCTGAAC TTCTCAGAGC ACCTGTACTT TGAGAACGGA
    CTGCAGAATC TGAAGGCTGG CGCCCAGCGG AGCCTCAAGA AGCTTAGGGA GAAGGTGGAC
    CAGAACCTCT GGATCATTGG GGCTGCCGTG GTGAACGCCT TCTACTCTCC AAACCGAAAC
    CAGATTGTGT TCCCCGCTGG GATCCTCCAG CCCCCTTTTT TCAGCAAGGA GCAGCCTCAA
    GCCTTGAACT TCGGGGGCAT CGGGATGGTC ATCGGGCACG AGATCACCCA CGGCTTTGAC
    GACAATGGCC GGAACTTCGA CAAGAATGGG AACATGCTGG ACTGGTGGAG CAACTTCTCT
    GCCCAGCACT TCAGGGAGCA GTCAGAGTGC ATGGTCTACC AATATGGCAA TTACTCCTGG
    GACCTGGCTG ATGACCAGAA TGTGAATGGC TTCAGCACAC TCGGGGAAAA CATTGCCGAC
    AATGGAGGGG TGCGGCAGGC ATACAAGGCT TACCTGAAGT GGGTGGCAGA AGGCGGCAAG
    GACCAGCAGC TGCCAGGCCT GGAACTGACC TACCAGCAAC TGTTCTTCAT CAACTATGCG
    CAGGTGTGGT GCGGATCCTA CCGGCCAGAG TTCGCCATCC AGTCCATCAA GACTGACGTC
    CACAGTCCCC TGAAGTACAG GGTGCTGGGC TCGCTGCAGA ACCTGGGCGC CTTCGCAGAC
    GCGTTCCACT GCGCGAATGG CTCCCCCATG CACCCAACGG TGCGATGCCG CGTCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223758
    Clone ID Related Accession (Same CDS sequence) XM_024976520.1
    Accession Version XM_024976520.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane metallo-endopeptidase-like 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCTGTGG TGGCCGCTCT GAGTGACCCA AAGAGACTTG CGATGGGCAA GCCCGAGAGC 
    CCAGTGGGGA TGGTGGAGAT CGCCGGCCGC TCGGGGCAGA AGCACCGAGG CTTCCTGGAG
    GCGGGGCTGC TGCTGCTGCT GCTCCTGGTG ACCGGCGCCC TGGTCACCCT GGGCCTCCTC
    TACGCCGACA GCAGAGGGAA GCGGCTGCCA CTCTTTTCTA GCCGGCTTTG CTTCTCCAAG
    GAAGAGAAGA ACACTGTAAA ACGAGCACCC CGAGAGATCA AACCCTCCAA GGACAATGTC
    GAGACCTGCA CCACCCCTGG CTGCGTGATG GCAGCTGCCA GGATCCTCCA GAACATGGAC
    CCATCCATGG AGCCGTGTAA CGACTTCTAC CAGTACGCAT GCGGGGGCTG GCTGCGGCGC
    CACGTGATCC CTGAGACCAA CTCGCGCTAC AGCGTCTTCG ACATCCTCCG GGATGAGCTG
    GAGATCATCC TCAAAGCGGT GCTGGAGAAC TCCACCACCA AAGACCGGCC GGCCGTGCAA
    AAGGCCAAGA TGCTCTACCG CTCCTGCATG AACGAAAGTG AGATAGAGAA GCGAGACTCA
    CAGCCCCTGC TGAATATCCT GGAGGTGGTG GGAGGCTGGC CAGTGGCCAT GGACAAGTGG
    AACAAGAGCG TAGGCCCCCA GTGGGAGCTG GAACAGCAGC TGGCCGTGAT GAACGCACAG
    TTCAACCGGC GGGTGCTCAT CGACCTCTTC ATCTGGAACG ACGACCAGAA CTCCAGCCGG
    CACATCATCT ACATAGACCA ACCCACCCTG GGCATGCCGT CCCGAGAGTA CTACTTCAAG
    GAGGGCAACA ACCAGAAGGT ACGGGAAGCC TACCTGCAGT TCATGGTGTC GGTGGCCATG
    ATGCTGCGGG CGGACTTGAA CCTCCCCGAG AACAGCGGCC TGGTTCAGGA AGACATGGCC
    CAGGTGCTGG AGCTGGAGAC GCAGCTGGCC AACGCCACGG TCCCCCAGGA AGAGCGCCAT
    GACGTCACCG CCCTGTACCA CAGGATGGAC CTGGAGCAGC TCCAGAACAG CTTCGGTCTG
    AAGGGATTTA ACTGGACTCT CTTCATACAA TCCGTGCTAT CCTCTGTCAA AATTGATCTG
    CTGCCTAATG AAGAAGTGGT GGTCTATGGC ATCCCCTACC TCCAGAATCT TGAAGGCATC
    ATCGATGTCT ACTCACCCAG GACCATGCAG AACTACCTGA TTTGGCGCCT GGTGCTGGAC
    CGCATCAGCA GCCTGAGCCA GCGGTTTAAG GAAGCGCGTG CAAGCTACCG CAAGGCACTC
    TATGGCACGA CGGTGGAGGA GGTTCGCTGG CGGGAGTGCG TCAGCTATGT CAACAGCAAC
    ATGGAGAGCG CCGTGGGCTC GCTCTATGTC AAGGAGGCCT TCTCCGGTGA CAGCAAGGAC
    GTGGTCAAAG AGCTCATTGA CAAGGTGCGG GCAGTGTTCG TGGACACTCT GGATGAGCTG
    AGCTGGATGG ATGAGTCATC CAAGAAGAAG GCCCAGGAGA AGGCCATGAA CATCCGGGAG
    CAGATTGGGT ACCCTGACTA CATCCTGGAG GAGGGGAACA AACATCTGGA CGAGGAGTAT
    TCCAACCTGA ACTTCTCAGA GCACCTGTAC TTTGAGAACG GACTGCAGAA TCTGAAGGCT
    GGCGCCCAGC GGAGCCTCAA GAAGCTTAGG GAGAAGGTGG ACCAGAACCT CTGGATCATT
    GGGGCTGCCG TGGTGAACGC CTTCTACTCT CCAAACCGAA ACCAGATTGT GTTCCCCGCT
    GGGATCCTCC AGCCCCCTTT TTTCAGCAAG GAGCAGCCTC AAGCCTTGAA CTTCGGGGGC
    ATCGGGATGG TCATCGGGCA CGAGATCACC CACGGCTTTG ACGACAATGG CCGGAACTTC
    GACAAGAATG GGAACATGCT GGACTGGTGG AGCAACTTCT CTGCCCAGCA CTTCAGGGAG
    CAGTCAGAGT GCATGGTCTA CCAATATGGC AATTACTCCT GGGACCTGGC TGATGACCAG
    AATGTGAATG GCTTCAGCAC ACTCGGGGAA AACATTGCCG ACAATGGAGG GGTGCGGCAG
    GCATACAAGG CTTACCTGAA GTGGGTGGCA GAAGGCGGCA AGGACCAGCA GCTGCCAGGC
    CTGGAACTGA CCTACCAGCA ACTGTTCTTC ATCAACTATG CGCAGTTCTG CAGCCACGCC
    CCTCATGCGC CCACAGGTGT GGTGCGGATC CTACCGGCCA GAGTTCGCCA TCCAGTCCAT
    CAAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832288.1
    CDS3711..5999
    Translation

    Target ORF information:

    RefSeq Version XM_024976520.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metalloendopeptidase like 1 (MMEL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976520.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCTGTGG TGGCCGCTCT GAGTGACCCA AAGAGACTTG CGATGGGCAA GCCCGAGAGC 
    CCAGTGGGGA TGGTGGAGAT CGCCGGCCGC TCGGGGCAGA AGCACCGAGG CTTCCTGGAG
    GCGGGGCTGC TGCTGCTGCT GCTCCTGGTG ACCGGCGCCC TGGTCACCCT GGGCCTCCTC
    TACGCCGACA GCAGAGGGAA GCGGCTGCCA CTCTTTTCTA GCCGGCTTTG CTTCTCCAAG
    GAAGAGAAGA ACACTGTAAA ACGAGCACCC CGAGAGATCA AACCCTCCAA GGACAATGTC
    GAGACCTGCA CCACCCCTGG CTGCGTGATG GCAGCTGCCA GGATCCTCCA GAACATGGAC
    CCATCCATGG AGCCGTGTAA CGACTTCTAC CAGTACGCAT GCGGGGGCTG GCTGCGGCGC
    CACGTGATCC CTGAGACCAA CTCGCGCTAC AGCGTCTTCG ACATCCTCCG GGATGAGCTG
    GAGATCATCC TCAAAGCGGT GCTGGAGAAC TCCACCACCA AAGACCGGCC GGCCGTGCAA
    AAGGCCAAGA TGCTCTACCG CTCCTGCATG AACGAAAGTG AGATAGAGAA GCGAGACTCA
    CAGCCCCTGC TGAATATCCT GGAGGTGGTG GGAGGCTGGC CAGTGGCCAT GGACAAGTGG
    AACAAGAGCG TAGGCCCCCA GTGGGAGCTG GAACAGCAGC TGGCCGTGAT GAACGCACAG
    TTCAACCGGC GGGTGCTCAT CGACCTCTTC ATCTGGAACG ACGACCAGAA CTCCAGCCGG
    CACATCATCT ACATAGACCA ACCCACCCTG GGCATGCCGT CCCGAGAGTA CTACTTCAAG
    GAGGGCAACA ACCAGAAGGT ACGGGAAGCC TACCTGCAGT TCATGGTGTC GGTGGCCATG
    ATGCTGCGGG CGGACTTGAA CCTCCCCGAG AACAGCGGCC TGGTTCAGGA AGACATGGCC
    CAGGTGCTGG AGCTGGAGAC GCAGCTGGCC AACGCCACGG TCCCCCAGGA AGAGCGCCAT
    GACGTCACCG CCCTGTACCA CAGGATGGAC CTGGAGCAGC TCCAGAACAG CTTCGGTCTG
    AAGGGATTTA ACTGGACTCT CTTCATACAA TCCGTGCTAT CCTCTGTCAA AATTGATCTG
    CTGCCTAATG AAGAAGTGGT GGTCTATGGC ATCCCCTACC TCCAGAATCT TGAAGGCATC
    ATCGATGTCT ACTCACCCAG GACCATGCAG AACTACCTGA TTTGGCGCCT GGTGCTGGAC
    CGCATCAGCA GCCTGAGCCA GCGGTTTAAG GAAGCGCGTG CAAGCTACCG CAAGGCACTC
    TATGGCACGA CGGTGGAGGA GGTTCGCTGG CGGGAGTGCG TCAGCTATGT CAACAGCAAC
    ATGGAGAGCG CCGTGGGCTC GCTCTATGTC AAGGAGGCCT TCTCCGGTGA CAGCAAGGAC
    GTGGTCAAAG AGCTCATTGA CAAGGTGCGG GCAGTGTTCG TGGACACTCT GGATGAGCTG
    AGCTGGATGG ATGAGTCATC CAAGAAGAAG GCCCAGGAGA AGGCCATGAA CATCCGGGAG
    CAGATTGGGT ACCCTGACTA CATCCTGGAG GAGGGGAACA AACATCTGGA CGAGGAGTAT
    TCCAACCTGA ACTTCTCAGA GCACCTGTAC TTTGAGAACG GACTGCAGAA TCTGAAGGCT
    GGCGCCCAGC GGAGCCTCAA GAAGCTTAGG GAGAAGGTGG ACCAGAACCT CTGGATCATT
    GGGGCTGCCG TGGTGAACGC CTTCTACTCT CCAAACCGAA ACCAGATTGT GTTCCCCGCT
    GGGATCCTCC AGCCCCCTTT TTTCAGCAAG GAGCAGCCTC AAGCCTTGAA CTTCGGGGGC
    ATCGGGATGG TCATCGGGCA CGAGATCACC CACGGCTTTG ACGACAATGG CCGGAACTTC
    GACAAGAATG GGAACATGCT GGACTGGTGG AGCAACTTCT CTGCCCAGCA CTTCAGGGAG
    CAGTCAGAGT GCATGGTCTA CCAATATGGC AATTACTCCT GGGACCTGGC TGATGACCAG
    AATGTGAATG GCTTCAGCAC ACTCGGGGAA AACATTGCCG ACAATGGAGG GGTGCGGCAG
    GCATACAAGG CTTACCTGAA GTGGGTGGCA GAAGGCGGCA AGGACCAGCA GCTGCCAGGC
    CTGGAACTGA CCTACCAGCA ACTGTTCTTC ATCAACTATG CGCAGTTCTG CAGCCACGCC
    CCTCATGCGC CCACAGGTGT GGTGCGGATC CTACCGGCCA GAGTTCGCCA TCCAGTCCAT
    CAAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27331
    Clone ID Related Accession (Same CDS sequence) XM_024976519.1
    Accession Version XM_024976519.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane metallo-endopeptidase-like 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCTGTGG TGGCCGCTCT GAGTGACCCA AAGAGACTTG CGATGGGCAA GCCCGAGAGC 
    CCAGTGGGGA TGGTGGAGAT CGCCGGCCGC TCGGGGCAGA AGCACCGAGG CTTCCTGGAG
    GCGGGGCTGC TGCTGCTGCT GCTCCTGGTG ACCGGCGCCC TGGTCACCCT GGGCCTCCTC
    TACGCCGACA GCAGAGGGAA GCGGCTGCCA CTCTTTTCTA GCCGGCTTTG CTTCTCCAAG
    GAAGAGAAGA ACACTGTAAA ACGAGCACCC CGAGAGATCA AACCCTCCAA GGACAATGTC
    GAGACCTGCA CCACCCCTGG CTGCGTGATG GCAGCTGCCA GGATCCTCCA GAACATGGAC
    CCATCCATGG AGCCGTGTAA CGACTTCTAC CAGTACGCAT GCGGGGGCTG GCTGCGGCGC
    CACGTGATCC CTGAGACCAA CTCGCGCTAC AGCGTCTTCG ACATCCTCCG GGATGAGCTG
    GAGATCATCC TCAAAGCGGT GCTGGAGAAC TCCACCACCA AAGACCGGCC GGCCGTGCAA
    AAGGCCAAGA TGCTCTACCG CTCCTGCATG AACGAAAGTG AGATAGAGAA GCGAGACTCA
    CAGCCCCTGC TGAATATCCT GGAGGTGGTG GGAGGCTGGC CAGTGGCCAT GGACAAGTGG
    AACAAGAGCG TAGGCCCCCA GTGGGAGCTG GAACAGCAGC TGGCCGTGAT GAACGCACAG
    TTCAACCGGC GGGTGCTCAT CGACCTCTTC ATCTGGAACG ACGACCAGAA CTCCAGCCGG
    CACATCATCT ACATAGACCA ACCCACCCTG GGCATGCCGT CCCGAGAGTA CTACTTCAAG
    GAGGGCAACA ACCAGAAGGT ACGGGAAGCC TACCTGCAGT TCATGGTGTC GGTGGCCATG
    ATGCTGCGGG CGGACTTGAA CCTCCCCGAG AACAGCGGCC TGGTTCAGGA AGACATGGCC
    CAGGTGCTGG AGCTGGAGAC GCAGCTGGCC AACGCCACGG TCCCCCAGGA AGAGCGCCAT
    GACGTCACCG CCCTGTACCA CAGGATGGAC CTGGAGCAGC TCCAGAACAG CTTCGGTCTG
    AAGGGATTTA ACTGGACTCT CTTCATACAA TCCGTGCTAT CCTCTGTCAA AATTGATCTG
    CTGCCTAATG AAGAAGTGGT GGTCTATGGC ATCCCCTACC TCCAGAATCT TGAAGGCATC
    ATCGATGTCT ACTCACCCAG GACCATGCAG AACTACCTGA TTTGGCGCCT GGTGCTGGAC
    CGCATCAGCA GCCTGAGCCA GCGGTTTAAG GAAGCGCGTG CAAGCTACCG CAAGGCACTC
    TATGGCACGA CGGTGGAGGA GGTTCGCTGG CGGGAGTGCG TCAGCTATGT CAACAGCAAC
    ATGGAGAGCG CCGTGGGCTC GCTCTATGTC AAGGAGGCCT TCTCCGGTGA CAGCAAGGAC
    GTGGTCAAAG AGCTCATTGA CAAGGTGCGG GCAGTGTTCG TGGACACTCT GGATGAGCTG
    AGCTGGATGG ATGAGTCATC CAAGAAGAAG GCCCAGGAGA AGGCCATGAA CATCCGGGAG
    CAGATTGGGT ACCCTGACTA CATCCTGGAG GAGGGGAACA AACATCTGGA CGAGGAGTAT
    TCCAACCTGA ACTTCTCAGA GCACCTGTAC TTTGAGAACG GACTGCAGAA TCTGAAGGCT
    GGCGCCCAGC GGAGCCTCAA GAAGCTTAGG GAGAAGGTGG ACCAGAACCT CTGGATCATT
    GGGGCTGCCG TGGTGAACGC CTTCTACTCT CCAAACCGAA ACCAGATTGT GTTCCCCGCT
    GGGATCCTCC AGCCCCCTTT TTTCAGCAAG GAGCAGCCTC AAGCCTTGAA CTTCGGGGGC
    ATCGGGATGG TCATCGGGCA CGAGATCACC CACGGCTTTG ACGACAATGG CCGGAACTTC
    GACAAGAATG GGAACATGCT GGACTGGTGG AGCAACTTCT CTGCCCAGCA CTTCAGGGAG
    CAGTCAGAGT GCATGGTCTA CCAATATGGC AATTACTCCT GGGACCTGGC TGATGACCAG
    AATGTGAATG GCTTCAGCAC ACTCGGGGAA AACATTGCCG ACAATGGAGG GGTGCGGCAG
    GCATACAAGG CTTACCTGAA GTGGGTGGCA GAAGGCGGCA AGGACCAGCA GCTGCCAGGC
    CTGGAACTGA CCTACCAGCA ACTGTTCTTC ATCAACTATG CGCAGGTGTG GTGCGGATCC
    TACCGGCCAG AGTTCGCCAT CCAGTCCATC AAGACTGACG TCCACAGTCC CCTGAAGTAC
    AGGGTGCTGG GCTCGCTGCA GAACCTGGGC GCCTTCGCAG ACGCGTTCCA CTGCGCGAAT
    GGCTCCCCCA TGCACCCAAC GGTGCGATGC CGCGTCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832287.1
    CDS195..2576
    Translation

    Target ORF information:

    RefSeq Version XM_024976519.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metalloendopeptidase like 1 (MMEL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976519.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCTGTGG TGGCCGCTCT GAGTGACCCA AAGAGACTTG CGATGGGCAA GCCCGAGAGC 
    CCAGTGGGGA TGGTGGAGAT CGCCGGCCGC TCGGGGCAGA AGCACCGAGG CTTCCTGGAG
    GCGGGGCTGC TGCTGCTGCT GCTCCTGGTG ACCGGCGCCC TGGTCACCCT GGGCCTCCTC
    TACGCCGACA GCAGAGGGAA GCGGCTGCCA CTCTTTTCTA GCCGGCTTTG CTTCTCCAAG
    GAAGAGAAGA ACACTGTAAA ACGAGCACCC CGAGAGATCA AACCCTCCAA GGACAATGTC
    GAGACCTGCA CCACCCCTGG CTGCGTGATG GCAGCTGCCA GGATCCTCCA GAACATGGAC
    CCATCCATGG AGCCGTGTAA CGACTTCTAC CAGTACGCAT GCGGGGGCTG GCTGCGGCGC
    CACGTGATCC CTGAGACCAA CTCGCGCTAC AGCGTCTTCG ACATCCTCCG GGATGAGCTG
    GAGATCATCC TCAAAGCGGT GCTGGAGAAC TCCACCACCA AAGACCGGCC GGCCGTGCAA
    AAGGCCAAGA TGCTCTACCG CTCCTGCATG AACGAAAGTG AGATAGAGAA GCGAGACTCA
    CAGCCCCTGC TGAATATCCT GGAGGTGGTG GGAGGCTGGC CAGTGGCCAT GGACAAGTGG
    AACAAGAGCG TAGGCCCCCA GTGGGAGCTG GAACAGCAGC TGGCCGTGAT GAACGCACAG
    TTCAACCGGC GGGTGCTCAT CGACCTCTTC ATCTGGAACG ACGACCAGAA CTCCAGCCGG
    CACATCATCT ACATAGACCA ACCCACCCTG GGCATGCCGT CCCGAGAGTA CTACTTCAAG
    GAGGGCAACA ACCAGAAGGT ACGGGAAGCC TACCTGCAGT TCATGGTGTC GGTGGCCATG
    ATGCTGCGGG CGGACTTGAA CCTCCCCGAG AACAGCGGCC TGGTTCAGGA AGACATGGCC
    CAGGTGCTGG AGCTGGAGAC GCAGCTGGCC AACGCCACGG TCCCCCAGGA AGAGCGCCAT
    GACGTCACCG CCCTGTACCA CAGGATGGAC CTGGAGCAGC TCCAGAACAG CTTCGGTCTG
    AAGGGATTTA ACTGGACTCT CTTCATACAA TCCGTGCTAT CCTCTGTCAA AATTGATCTG
    CTGCCTAATG AAGAAGTGGT GGTCTATGGC ATCCCCTACC TCCAGAATCT TGAAGGCATC
    ATCGATGTCT ACTCACCCAG GACCATGCAG AACTACCTGA TTTGGCGCCT GGTGCTGGAC
    CGCATCAGCA GCCTGAGCCA GCGGTTTAAG GAAGCGCGTG CAAGCTACCG CAAGGCACTC
    TATGGCACGA CGGTGGAGGA GGTTCGCTGG CGGGAGTGCG TCAGCTATGT CAACAGCAAC
    ATGGAGAGCG CCGTGGGCTC GCTCTATGTC AAGGAGGCCT TCTCCGGTGA CAGCAAGGAC
    GTGGTCAAAG AGCTCATTGA CAAGGTGCGG GCAGTGTTCG TGGACACTCT GGATGAGCTG
    AGCTGGATGG ATGAGTCATC CAAGAAGAAG GCCCAGGAGA AGGCCATGAA CATCCGGGAG
    CAGATTGGGT ACCCTGACTA CATCCTGGAG GAGGGGAACA AACATCTGGA CGAGGAGTAT
    TCCAACCTGA ACTTCTCAGA GCACCTGTAC TTTGAGAACG GACTGCAGAA TCTGAAGGCT
    GGCGCCCAGC GGAGCCTCAA GAAGCTTAGG GAGAAGGTGG ACCAGAACCT CTGGATCATT
    GGGGCTGCCG TGGTGAACGC CTTCTACTCT CCAAACCGAA ACCAGATTGT GTTCCCCGCT
    GGGATCCTCC AGCCCCCTTT TTTCAGCAAG GAGCAGCCTC AAGCCTTGAA CTTCGGGGGC
    ATCGGGATGG TCATCGGGCA CGAGATCACC CACGGCTTTG ACGACAATGG CCGGAACTTC
    GACAAGAATG GGAACATGCT GGACTGGTGG AGCAACTTCT CTGCCCAGCA CTTCAGGGAG
    CAGTCAGAGT GCATGGTCTA CCAATATGGC AATTACTCCT GGGACCTGGC TGATGACCAG
    AATGTGAATG GCTTCAGCAC ACTCGGGGAA AACATTGCCG ACAATGGAGG GGTGCGGCAG
    GCATACAAGG CTTACCTGAA GTGGGTGGCA GAAGGCGGCA AGGACCAGCA GCTGCCAGGC
    CTGGAACTGA CCTACCAGCA ACTGTTCTTC ATCAACTATG CGCAGGTGTG GTGCGGATCC
    TACCGGCCAG AGTTCGCCAT CCAGTCCATC AAGACTGACG TCCACAGTCC CCTGAAGTAC
    AGGGTGCTGG GCTCGCTGCA GAACCTGGGC GCCTTCGCAG ACGCGTTCCA CTGCGCGAAT
    GGCTCCCCCA TGCACCCAAC GGTGCGATGC CGCGTCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa56190
    Clone ID Related Accession (Same CDS sequence) XM_024976521.1
    Accession Version XM_024976521.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane metallo-endopeptidase-like 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTGTGG TGGCCGCTCT GAGTGACCCA AAGAGACTTG CGATGGGCAA GCCCGAGAGC 
    CCAGTGGGGA TGGTGGAGAT CGCCGGCCGC TCGGGGCAGA AGCACCGAGG CTTCCTGGAG
    GCGGGGCTGC TGCTGCTGCT GCTCCTGGTG ACCGGCGCCC TGGTCACCCT GGGCCTCCTC
    TACGCCGACA GCAGAGGGAA GCGGCTGCCA CTCTTTTCTA GCCGGCTTTG CTTCTCCAAG
    GAAGAGAAGA ACACTGTAAA ACGAGCACCC CGAGAGATCA AACCCTCCAA GGACAATGTC
    GAGACCTGCA CCACCCCTGG CTGCGTGATG GCAGCTGCCA GGATCCTCCA GAACATGGAC
    CCATCCATGG AGCCGTGTAA CGACTTCTAC CAGTACGCAT GCGGGGGCTG GCTGCGGCGC
    CACGTGATCC CTGAGACCAA CTCGCGCTAC AGCGTCTTCG ACATCCTCCG GGATGAGCTG
    GAGATCATCC TCAAAGCGGT GCTGGAGAAC TCCACCACCA AAGACCGGCC GGCCGTGCAA
    AAGGCCAAGA TGCTCTACCG CTCCTGCATG AACGAAAGTG AGATAGAGAA GCGAGACTCA
    CAGCCCCTGC TGAATATCCT GGAGGTGGTG GGAGGCTGGC CAGTGGCCAT GGACAAGTGG
    AACAAGAGCG TAGGCCCCCA GTGGGAGCTG GAACAGCAGC TGGCCGTGAT GAACGCACAG
    TTCAACCGGC GGGTGCTCAT CGACCTCTTC ATCTGGAACG ACGACCAGAA CTCCAGCCGG
    CACATCATCT ACATAGACCA ACCCACCCTG GGCATGCCGT CCCGAGAGTA CTACTTCAAG
    GAGGGCAACA ACCAGAAGGT ACGGGAAGCC TACCTGCAGT TCATGGTGTC GGTGGCCATG
    ATGCTGCGGG CGGACTTGAA CCTCCCCGAG AACAGCGGCC TGGTTCAGGA AGACATGGCC
    CAGGTGCTGG AGCTGGAGAC GCAGCTGGCC AACGCCACGG TCCCCCAGGA AGAGCGCCAT
    GACGTCACCG CCCTGTACCA CAGGATGGAC CTGGAGCAGC TCCAGAACAG CTTCGGTCTG
    AAGGGATTTA ACTGGACTCT CTTCATACAA TCCGTGCTAT CCTCTGTCAA AATTGATCTG
    CTGCCTAATG AAGAAGTGGT GGTCTATGGC ATCCCCTACC TCCAGAATCT TGAAGGCATC
    ATCGATGTCT ACTCACCCAG GACCATGCAG AACTACCTGA TTTGGCGCCT GGTGCTGGAC
    CGCATCAGCA GCCTGAGCCA GCGGTTTAAG GAAGCGCGTG CAAGCTACCG CAAGGCACTC
    TATGGCACGA CGGTGGAGGA GGTTCGCTGG CGGGAGTGCG TCAGCTATGT CAACAGCAAC
    ATGGAGAGCG CCGTGGGCTC GCTCTATGTC AAGGAGGCCT TCTCCGGTGA CAGCAAGGAC
    GTGGTCAAAG AGCTCATTGA CAAGGTGCGG GCAGTGTTCG TGGACACTCT GGATGAGCTG
    AGCTGGATGG ATGAGTCATC CAAGAAGAAG GCCCAGGAGA AGGCCATGAA CATCCGGGAG
    CAGATTGGGT ACCCTGACTA CATCCTGGAG GAGGGGAACA AACATCTGGA CGAGGAGTAT
    TCCAACCTGA ACTTCTCAGA GCACCTGTAC TTTGAGAACG GACTGCAGAA TCTGAAGGCT
    GGCGCCCAGC GGAGCCTCAA GAAGCTTAGG GAGAAGGTGG ACCAGAACCT CTGGATCATT
    GGGGCTGCCG TGGTGAACGC CTTCTACTCT CCAAACCGAA ACCAGATTGG CCGGAACTTC
    GACAAGAATG GGAACATGCT GGACTGGTGG AGCAACTTCT CTGCCCAGCA CTTCAGGGAG
    CAGTCAGAGT GCATGGTCTA CCAATATGGC AATTACTCCT GGGACCTGGC TGATGACCAG
    AATGTGAATG GCTTCAGCAC ACTCGGGGAA AACATTGCCG ACAATGGAGG GGTGCGGCAG
    GCATACAAGG CTTACCTGAA GTGGGTGGCA GAAGGCGGCA AGGACCAGCA GCTGCCAGGC
    CTGGAACTGA CCTACCAGCA ACTGTTCTTC ATCAACTATG CGCAGGTGTG GTGCGGATCC
    TACCGGCCAG AGTTCGCCAT CCAGTCCATC AAGACTGACG TCCACAGTCC CCTGAAGTAC
    AGGGTGCTGG GCTCGCTGCA GAACCTGGGC GCCTTCGCAG ACGCGTTCCA CTGCGCGAAT
    GGCTCCCCCA TGCACCCAAC GGTGCGATGC CGCGTCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832289.1
    CDS3712..5973
    Translation

    Target ORF information:

    RefSeq Version XM_024976521.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metalloendopeptidase like 1 (MMEL1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976521.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTGTGG TGGCCGCTCT GAGTGACCCA AAGAGACTTG CGATGGGCAA GCCCGAGAGC 
    CCAGTGGGGA TGGTGGAGAT CGCCGGCCGC TCGGGGCAGA AGCACCGAGG CTTCCTGGAG
    GCGGGGCTGC TGCTGCTGCT GCTCCTGGTG ACCGGCGCCC TGGTCACCCT GGGCCTCCTC
    TACGCCGACA GCAGAGGGAA GCGGCTGCCA CTCTTTTCTA GCCGGCTTTG CTTCTCCAAG
    GAAGAGAAGA ACACTGTAAA ACGAGCACCC CGAGAGATCA AACCCTCCAA GGACAATGTC
    GAGACCTGCA CCACCCCTGG CTGCGTGATG GCAGCTGCCA GGATCCTCCA GAACATGGAC
    CCATCCATGG AGCCGTGTAA CGACTTCTAC CAGTACGCAT GCGGGGGCTG GCTGCGGCGC
    CACGTGATCC CTGAGACCAA CTCGCGCTAC AGCGTCTTCG ACATCCTCCG GGATGAGCTG
    GAGATCATCC TCAAAGCGGT GCTGGAGAAC TCCACCACCA AAGACCGGCC GGCCGTGCAA
    AAGGCCAAGA TGCTCTACCG CTCCTGCATG AACGAAAGTG AGATAGAGAA GCGAGACTCA
    CAGCCCCTGC TGAATATCCT GGAGGTGGTG GGAGGCTGGC CAGTGGCCAT GGACAAGTGG
    AACAAGAGCG TAGGCCCCCA GTGGGAGCTG GAACAGCAGC TGGCCGTGAT GAACGCACAG
    TTCAACCGGC GGGTGCTCAT CGACCTCTTC ATCTGGAACG ACGACCAGAA CTCCAGCCGG
    CACATCATCT ACATAGACCA ACCCACCCTG GGCATGCCGT CCCGAGAGTA CTACTTCAAG
    GAGGGCAACA ACCAGAAGGT ACGGGAAGCC TACCTGCAGT TCATGGTGTC GGTGGCCATG
    ATGCTGCGGG CGGACTTGAA CCTCCCCGAG AACAGCGGCC TGGTTCAGGA AGACATGGCC
    CAGGTGCTGG AGCTGGAGAC GCAGCTGGCC AACGCCACGG TCCCCCAGGA AGAGCGCCAT
    GACGTCACCG CCCTGTACCA CAGGATGGAC CTGGAGCAGC TCCAGAACAG CTTCGGTCTG
    AAGGGATTTA ACTGGACTCT CTTCATACAA TCCGTGCTAT CCTCTGTCAA AATTGATCTG
    CTGCCTAATG AAGAAGTGGT GGTCTATGGC ATCCCCTACC TCCAGAATCT TGAAGGCATC
    ATCGATGTCT ACTCACCCAG GACCATGCAG AACTACCTGA TTTGGCGCCT GGTGCTGGAC
    CGCATCAGCA GCCTGAGCCA GCGGTTTAAG GAAGCGCGTG CAAGCTACCG CAAGGCACTC
    TATGGCACGA CGGTGGAGGA GGTTCGCTGG CGGGAGTGCG TCAGCTATGT CAACAGCAAC
    ATGGAGAGCG CCGTGGGCTC GCTCTATGTC AAGGAGGCCT TCTCCGGTGA CAGCAAGGAC
    GTGGTCAAAG AGCTCATTGA CAAGGTGCGG GCAGTGTTCG TGGACACTCT GGATGAGCTG
    AGCTGGATGG ATGAGTCATC CAAGAAGAAG GCCCAGGAGA AGGCCATGAA CATCCGGGAG
    CAGATTGGGT ACCCTGACTA CATCCTGGAG GAGGGGAACA AACATCTGGA CGAGGAGTAT
    TCCAACCTGA ACTTCTCAGA GCACCTGTAC TTTGAGAACG GACTGCAGAA TCTGAAGGCT
    GGCGCCCAGC GGAGCCTCAA GAAGCTTAGG GAGAAGGTGG ACCAGAACCT CTGGATCATT
    GGGGCTGCCG TGGTGAACGC CTTCTACTCT CCAAACCGAA ACCAGATTGG CCGGAACTTC
    GACAAGAATG GGAACATGCT GGACTGGTGG AGCAACTTCT CTGCCCAGCA CTTCAGGGAG
    CAGTCAGAGT GCATGGTCTA CCAATATGGC AATTACTCCT GGGACCTGGC TGATGACCAG
    AATGTGAATG GCTTCAGCAC ACTCGGGGAA AACATTGCCG ACAATGGAGG GGTGCGGCAG
    GCATACAAGG CTTACCTGAA GTGGGTGGCA GAAGGCGGCA AGGACCAGCA GCTGCCAGGC
    CTGGAACTGA CCTACCAGCA ACTGTTCTTC ATCAACTATG CGCAGGTGTG GTGCGGATCC
    TACCGGCCAG AGTTCGCCAT CCAGTCCATC AAGACTGACG TCCACAGTCC CCTGAAGTAC
    AGGGTGCTGG GCTCGCTGCA GAACCTGGGC GCCTTCGCAG ACGCGTTCCA CTGCGCGAAT
    GGCTCCCCCA TGCACCCAAC GGTGCGATGC CGCGTCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92975
    Clone ID Related Accession (Same CDS sequence) XM_015475156.1
    Accession Version XM_015475156.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane metallo-endopeptidase-like 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGGCAAGC CCGAGAGCCC AGTGGGGATG GTGGAGATCG CCGGCCGCTC GGGGCAGAAG 
    CACCGAGGCT TCCTGGAGGC GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTCACCCTGG GCCTCCTCTA CGCCGACAGC AGAGGGAAGC GGCTGCCACT CTTTTCTAGC
    CGGCTTTGCT TCTCCAAGGA AGAGAAGAAC ACTGTAAAAC GAGCACCCCG AGAGATCAAA
    CCCTCCAAGG ACAATGTCGA GACCTGCACC ACCCCTGGCT GCGTGATGGC AGCTGCCAGG
    ATCCTCCAGA ACATGGACCC ATCCATGGAG CCGTGTAACG ACTTCTACCA GTACGCATGC
    GGGGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CGCGCTACAG CGTCTTCGAC
    ATCCTCCGGG ATGAGCTGGA GATCATCCTC AAAGCGGTGC TGGAGAACTC CACCACCAAA
    GACCGGCCGG CCGTGCAAAA GGCCAAGATG CTCTACCGCT CCTGCATGAA CGAAAGTGAG
    ATAGAGAAGC GAGACTCACA GCCCCTGCTG AATATCCTGG AGGTGGTGGG AGGCTGGCCA
    GTGGCCATGG ACAAGTGGAA CAAGAGCGTA GGCCCCCAGT GGGAGCTGGA ACAGCAGCTG
    GCCGTGATGA ACGCACAGTT CAACCGGCGG GTGCTCATCG ACCTCTTCAT CTGGAACGAC
    GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAAC CCACCCTGGG CATGCCGTCC
    CGAGAGTACT ACTTCAAGGA GGGCAACAAC CAGAAGGTAC GGGAAGCCTA CCTGCAGTTC
    ATGGTGTCGG TGGCCATGAT GCTGCGGGCG GACTTGAACC TCCCCGAGAA CAGCGGCCTG
    GTTCAGGAAG ACATGGCCCA GGTGCTGGAG CTGGAGACGC AGCTGGCCAA CGCCACGGTC
    CCCCAGGAAG AGCGCCATGA CGTCACCGCC CTGTACCACA GGATGGACCT GGAGCAGCTC
    CAGAACAGCT TCGGTCTGAA GGGATTTAAC TGGACTCTCT TCATACAATC CGTGCTATCC
    TCTGTCAAAA TTGATCTGCT GCCTAATGAA GAAGTGGTGG TCTATGGCAT CCCCTACCTC
    CAGAATCTTG AAGGCATCAT CGATGTCTAC TCACCCAGGA CCATGCAGAA CTACCTGATT
    TGGCGCCTGG TGCTGGACCG CATCAGCAGC CTGAGCCAGC GGTTTAAGGA AGCGCGTGCA
    AGCTACCGCA AGGCACTCTA TGGCACGACG GTGGAGGAGG TTCGCTGGCG GGAGTGCGTC
    AGCTATGTCA ACAGCAACAT GGAGAGCGCC GTGGGCTCGC TCTATGTCAA GGAGGCCTTC
    TCCGGTGACA GCAAGGACGT GGTCAAAGAG CTCATTGACA AGGTGCGGGC AGTGTTCGTG
    GACACTCTGG ATGAGCTGAG CTGGATGGAT GAGTCATCCA AGAAGAAGGC CCAGGAGAAG
    GCCATGAACA TCCGGGAGCA GATTGGGTAC CCTGACTACA TCCTGGAGGA GGGGAACAAA
    CATCTGGACG AGGAGTATTC CAACCTGAAC TTCTCAGAGC ACCTGTACTT TGAGAACGGA
    CTGCAGAATC TGAAGGCTGG CGCCCAGCGG AGCCTCAAGA AGCTTAGGGA GAAGGTGGAC
    CAGAACCTCT GGATCATTGG GGCTGCCGTG GTGAACGCCT TCTACTCTCC AAACCGAAAC
    CAGATTGTGT TCCCCGCTGG GATCCTCCAG CCCCCTTTTT TCAGCAAGGA GCAGCCTCAA
    GCCTTGAACT TCGGGGGCAT CGGGATGGTC ATCGGGCACG AGATCACCCA CGGCTTTGAC
    GACAATGGCC GGAACTTCGA CAAGAATGGG AACATGCTGG ACTGGTGGAG CAACTTCTCT
    GCCCAGCACT TCAGGGAGCA GTCAGAGTGC ATGGTCTACC AATATGGCAA TTACTCCTGG
    GACCTGGCTG ATGACCAGAA TGTGAATGGC TTCAGCACAC TCGGGGAAAA CATTGCCGAC
    AATGGAGGGG TGCGGCAGGC ATACAAGGCT TACCTGAAGT GGGTGGCAGA AGGCGGCAAG
    GACCAGCAGC TGCCAGGCCT GGAACTGACC TACCAGCAAC TGTTCTTCAT CAACTATGCG
    CAGGTGTGGT GCGGATCCTA CCGGCCAGAG TTCGCCATCC AGTCCATCAA GACTGACGTC
    CACAGTCCCC TGAAGTACAG GCAAGTGACC CTGGCGTCCC CGCGCCCCGC GCCCAGGCCG
    GCGCTGTCTC AGGCCCCTCC GTCCACCCAC AGGGTGCTGG GCTCGCTGCA GAACCTGGGC
    GCCTTCGCAG ACGCGTTCCA CTGCGCGAAT GGCTCCCCCA TGCACCCAAC GGTGCGATGC
    CGCGTCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330642.1
    CDS1..2412
    Translation

    Target ORF information:

    RefSeq Version XM_015475156.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metallo-endopeptidase-like 1 (MMEL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGGCAAGC CCGAGAGCCC AGTGGGGATG GTGGAGATCG CCGGCCGCTC GGGGCAGAAG 
    CACCGAGGCT TCCTGGAGGC GGGGCTGCTG CTGCTGCTGC TCCTGGTGAC CGGCGCCCTG
    GTCACCCTGG GCCTCCTCTA CGCCGACAGC AGAGGGAAGC GGCTGCCACT CTTTTCTAGC
    CGGCTTTGCT TCTCCAAGGA AGAGAAGAAC ACTGTAAAAC GAGCACCCCG AGAGATCAAA
    CCCTCCAAGG ACAATGTCGA GACCTGCACC ACCCCTGGCT GCGTGATGGC AGCTGCCAGG
    ATCCTCCAGA ACATGGACCC ATCCATGGAG CCGTGTAACG ACTTCTACCA GTACGCATGC
    GGGGGCTGGC TGCGGCGCCA CGTGATCCCT GAGACCAACT CGCGCTACAG CGTCTTCGAC
    ATCCTCCGGG ATGAGCTGGA GATCATCCTC AAAGCGGTGC TGGAGAACTC CACCACCAAA
    GACCGGCCGG CCGTGCAAAA GGCCAAGATG CTCTACCGCT CCTGCATGAA CGAAAGTGAG
    ATAGAGAAGC GAGACTCACA GCCCCTGCTG AATATCCTGG AGGTGGTGGG AGGCTGGCCA
    GTGGCCATGG ACAAGTGGAA CAAGAGCGTA GGCCCCCAGT GGGAGCTGGA ACAGCAGCTG
    GCCGTGATGA ACGCACAGTT CAACCGGCGG GTGCTCATCG ACCTCTTCAT CTGGAACGAC
    GACCAGAACT CCAGCCGGCA CATCATCTAC ATAGACCAAC CCACCCTGGG CATGCCGTCC
    CGAGAGTACT ACTTCAAGGA GGGCAACAAC CAGAAGGTAC GGGAAGCCTA CCTGCAGTTC
    ATGGTGTCGG TGGCCATGAT GCTGCGGGCG GACTTGAACC TCCCCGAGAA CAGCGGCCTG
    GTTCAGGAAG ACATGGCCCA GGTGCTGGAG CTGGAGACGC AGCTGGCCAA CGCCACGGTC
    CCCCAGGAAG AGCGCCATGA CGTCACCGCC CTGTACCACA GGATGGACCT GGAGCAGCTC
    CAGAACAGCT TCGGTCTGAA GGGATTTAAC TGGACTCTCT TCATACAATC CGTGCTATCC
    TCTGTCAAAA TTGATCTGCT GCCTAATGAA GAAGTGGTGG TCTATGGCAT CCCCTACCTC
    CAGAATCTTG AAGGCATCAT CGATGTCTAC TCACCCAGGA CCATGCAGAA CTACCTGATT
    TGGCGCCTGG TGCTGGACCG CATCAGCAGC CTGAGCCAGC GGTTTAAGGA AGCGCGTGCA
    AGCTACCGCA AGGCACTCTA TGGCACGACG GTGGAGGAGG TTCGCTGGCG GGAGTGCGTC
    AGCTATGTCA ACAGCAACAT GGAGAGCGCC GTGGGCTCGC TCTATGTCAA GGAGGCCTTC
    TCCGGTGACA GCAAGGACGT GGTCAAAGAG CTCATTGACA AGGTGCGGGC AGTGTTCGTG
    GACACTCTGG ATGAGCTGAG CTGGATGGAT GAGTCATCCA AGAAGAAGGC CCAGGAGAAG
    GCCATGAACA TCCGGGAGCA GATTGGGTAC CCTGACTACA TCCTGGAGGA GGGGAACAAA
    CATCTGGACG AGGAGTATTC CAACCTGAAC TTCTCAGAGC ACCTGTACTT TGAGAACGGA
    CTGCAGAATC TGAAGGCTGG CGCCCAGCGG AGCCTCAAGA AGCTTAGGGA GAAGGTGGAC
    CAGAACCTCT GGATCATTGG GGCTGCCGTG GTGAACGCCT TCTACTCTCC AAACCGAAAC
    CAGATTGTGT TCCCCGCTGG GATCCTCCAG CCCCCTTTTT TCAGCAAGGA GCAGCCTCAA
    GCCTTGAACT TCGGGGGCAT CGGGATGGTC ATCGGGCACG AGATCACCCA CGGCTTTGAC
    GACAATGGCC GGAACTTCGA CAAGAATGGG AACATGCTGG ACTGGTGGAG CAACTTCTCT
    GCCCAGCACT TCAGGGAGCA GTCAGAGTGC ATGGTCTACC AATATGGCAA TTACTCCTGG
    GACCTGGCTG ATGACCAGAA TGTGAATGGC TTCAGCACAC TCGGGGAAAA CATTGCCGAC
    AATGGAGGGG TGCGGCAGGC ATACAAGGCT TACCTGAAGT GGGTGGCAGA AGGCGGCAAG
    GACCAGCAGC TGCCAGGCCT GGAACTGACC TACCAGCAAC TGTTCTTCAT CAACTATGCG
    CAGGTGTGGT GCGGATCCTA CCGGCCAGAG TTCGCCATCC AGTCCATCAA GACTGACGTC
    CACAGTCCCC TGAAGTACAG GCAAGTGACC CTGGCGTCCC CGCGCCCCGC GCCCAGGCCG
    GCGCTGTCTC AGGCCCCTCC GTCCACCCAC AGGGTGCTGG GCTCGCTGCA GAACCTGGGC
    GCCTTCGCAG ACGCGTTCCA CTGCGCGAAT GGCTCCCCCA TGCACCCAAC GGTGCGATGC
    CGCGTCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.