Nep4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nep4
Entrez Gene ID	42449
Full Name	Neprilysin 4
Synonyms	CG4058,DmeNEP4,Dmel\CG4058,NEP4,dNEP4,nep4
General protein information	Preferred Names Neprilysin 4 Names CG4058 gene product from transcript CG4058-RC CG4058-PA CG4058-PB CG4058-PC Nep4-PA Nep4-PB Nep4-PC neprilysin 4 neprilysin4
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92F4-92F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_142647.3	NP_650904.3	neprilysin 4, isoform A
NM_001275844.1	NP_001262773.1	neprilysin 4, isoform C
NM_169912.1	NP_732540.2	neprilysin 4, isoform B

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MMEL1	XP_001233077.3
Danio rerio (zebrafish)	mmel1	XP_689191.3
Canis lupus familiaris (dog)	MMEL1	XP_005620485.1
Mus musculus (house mouse)	Mmel1	NP_038811.2
Xenopus tropicalis (tropical clawed frog)	mmel1	NP_001120567.1
Homo sapiens (human)	MMEL1	NP_258428.2
Drosophila melanogaster (fruit fly)	Nep4	NP_650904.3
Pan troglodytes (chimpanzee)	MMEL1	XP_001150860.1
Bos taurus (cattle)	MMEL1	NP_001180065.1
Rattus norvegicus (Norway rat)	Mmel1	NP_001101467.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003106	XP_312790.4
Caenorhabditis elegans (roundworm)	nep-2	NP_494343.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Herein, the authors show that in Drosophila expression of insulin-like peptides is regulated by neprilysin 4 activity.

The NEP4 involved in middle-term memory (MTM) and long-term memory (LTM) and that their expression is required in the mushroom bodies and also in a single pair of closely connected neurons.
Title: Drosophila Neprilysins Are Involved in Middle-Term and Long-Term Memory.

Overexpression of Nep4 leads to a severe muscle degeneration.
Title: A novel role for the non-catalytic intracellular domain of Neprilysins in muscle physiology.

Results report the identification and first characterization of neprilysin 4 (NEP4) from Drosophila melanogaster.
Title: Neprilysin 4, a novel endopeptidase from Drosophila melanogaster, displays distinct substrate specificities and exceptional solubility states.

The following Nep4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nep4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05498	NM_169912.1 Latest version!	Drosophila melanogaster neprilysin 4, transcript variant B (Nep4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa05498	NM_001275844.1 Latest version!	Drosophila melanogaster neprilysin 4, transcript variant C (Nep4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa05499	NM_142647.3 Latest version!	Drosophila melanogaster neprilysin 4, transcript variant A (Nep4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nep4 ( NM_001275844.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262773.1 Drosophila melanogaster neprilysin 4, transcript variant C (Nep4), mRNA.

Price & Availability↑

CloneID	OFa05498
Clone ID Related Accession (Same CDS sequence)	NM_001275844.1 , NM_169912.1
Accession Version	NM_001275844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neprilysin 4, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTAATGC TGCCACTGAC CCTGGTGCTC ATCCTGATCA TGCGGCTGGA CGGGATGCTG 
GCGGCGCTGC AATTGAACGA ACAGAGGATG AGGGATCTGC GGAACTCTCA CAGCGAGGTG 
CCTGTCTATA TGGAGGATTA CGAAGCCCTT TTACCGGAGG GTAGTACCTA CAACGACCTG 
ATCAACGAGG AGTTCATACT GCCGGCGAGC AAGCGGACCC AACTGCAGAT TTTGGCCGCG 
GAGAGGGCGC GTCGCTGCCA ACCATATCGC TACGGGAACG GGGAGTCCAT GGAGTTGGAG 
GAGCGCAACA CGCTGATGAA GGACTCCCGA ACCTCCTTCC TGCCACTGGG CATTCCGCGA 
GAGTGCCTCG GCAGCGGCAT TGAACTGGAC ATTAAACCCA TAGATGAGGA GGCCTACCAG 
AGGCAGAAGA AGCGCTACCA GGACATAGCT CCGTATTGGC TGGAGAAGAT CAGAATACGG 
GAGCGCCGCG AGGCCGAACG TCATGCAGAG GAGGCCAGTG CCGAGATCAG CGAGGCCACC 
GCCGCTCTGC AGAGTTTCTG GAACGAGGAA GGCACTCGGG AGGGCATTCG CATGACCCAG 
GCCAAAACCA TGAAGCGCTA CATGGATAAC AAAGTGGATC CCTGCGTGGA TTTCTACAAG 
TACGCCTGCG GCAACTGGGA GCGCCTGCAT CCAATACCCA AGGATAAGGC CGGCTTCGAT 
ACCTTCGAAA TGCTGCGTGA GAGTCTGGAC CTGGTGCTAC GAAATCTTCT GGAGAAAAAT 
ACCCCTGTGC ACTCCGCAGC GGAGCTACGA AAAAGTCCCG TGCGGAATAC TCTATTTAAG 
CTTAATGAGC AGGGCGAGGG TGAGGGCGAA GCTGACCAGG CGGCGGAACT TACGGCGGAA 
CGCCTGCGTC GGCACATTGT CAGTAAGAGG CAGTTGCTTA ACCGCGTTCT GGTGCGCTAC 
AAGCGGTATA CCAACGGAAC GAAAAGGAAA CGCCTCATCG AAACCCCACG GGAGAGAACC 
AAAGAGGAGG AAGCTGCTCC ACCAGTTGTC CTGCCAAAGG ATAAAACCAA GGACAAGTCG 
GATAACGAAG AGCAACTACA TGTGCCCACC GATTTCCTAA AGCCCCATCA AGATGCCCAG 
CTAAAAGCAA AGAATCTGTA CCGGTCCTGC GTGAACAGCG CGGTGCTGGC CAAGCGGGGA 
TTGGAACCGC TGCACACGCT CATTCGGGAA CTAGGTGGCT GGCCAGTCCT GGAGTCCCAA 
TGGAGTGAGT CCAACTTTAA CTGGCAGGTG CTAGCCGCCA CCCTTAGACG TTACAACAAC 
GACATCCTCA TCGTCCAGTG GGTGGGGGCG GACATCAAGA ACTCCGAGGA GAACATTGTT 
CAGTTTGACC AGACGGGTCT GGGCCTGCCC ACCAGGGAGT ACTTCCTCCA GCCGAGCAAC 
GCCAAGTACC TACAGGCCTA CCAGCGCTAC ATGGCCGAGG TGATGCACAA AATGGGCGCC 
TCCAAGGCGG ATGCCCAGCG GGTGGCCAGC GAGCTGGTGG CGTTCGAAAC GCAGCTGGCG 
GGGATTACAG CTCCAGCGGA GCAACGACTT AACGTCACCA AGCTCTACAA GCGCATGACG 
CTGGACCAAC TGCAGGCGGT GGTTCCGGAA ATCAAGTGGC GAGCTTACCT GCAGAGTCTG 
CAAGATCGCG AAGTTCTGGG CACCGAGGAA GTGGTCATCT ACGCGGTGGA GTACATGAGC 
AAGCTGGTGA CTCTTCTAGA TGAAACGGAT CCCCGAACGG TTTCCAATTA CATGATGTGG 
CGCTTTGTGC GGCACAGGAT CAACAACGTG GACGATCGAT TCGATGACAT CAAACAGAGT 
TTCTACCACG CCCTCTTTGG CCGCGAGGAG AGTCCGCAGA GATGGAAGGT ATGCATCGCC 
CAGGTGAACA CCAACATGGG CATGGCAGTG GGTTCCATGT TCGTGAGTCG CTATTTCGAC 
AACAACAGCA AGCGGGACAC CTTGCGGATG ACTCACGATC TGCAGCAGGC CTTTCGGGAT 
ATCCTGAAAA CCACTGATTG GCTGGACGAC ACCACAAAGC AGTTGGCCGA GGAAAAGGTT 
AACGCCATGT CCCTGAAGAT CGGCTATCCA GACTTTATCC TTAATCCCAG TGAGTTAAAT 
AGCAAGTACG CGGGCATAGA AATCTATCCG GAAAAGTATT TTGAGAACAC TCTGAATGTT 
CTGCTTCACA CGGCCAAAAC GGAGCAGGCC AAGCTCCACG AGAGGGTCAA CAAGACCAAC 
TGGCAGACAG CTCCTGCCAT CGTCAATGCC TATTATAGCC GCAACAAGAA CCAGATCATG 
TTTCCCGCCG GCATCCTGCA GCCGCCCTTC TACCACCGCC ACTTTCCGAA GTCGCTGAAC 
TTCGGCGGCA TCGGTGTGGT CATTGGCCAC GAACTGACCC ACGGTTTCGA TGACAAGGGT 
AGGCTCTTCG ATCGCAACGG CAATATACAT AAGTGGTGGA CGGACTCCTC GATTCGCGGG 
TTCGACGAGC GCGCCCGCTG CATTATTGCC CAATATAGCA ACTATACCGT CGAGGAGGTG 
GGAATCGTCC TGAATGGAGA GAGTACGCAG GGTGAGAATA TCGCAGACAA TGGAGGCCTC 
CGGCAGGCCT TCCACGCGTA TCAGCGCTGG CTAAAGGAGC ATCCCAGCGA GGTGTCGGAC 
GAGATCCTGC CAGGACTTAA CATGACAGGG CCGCAGCTGT TCTTCCTGAA CTTCGGTCAG 
GTTTGGTGCG GAGCGATGCG ACCGGAGGCC ATCCGGAACA AGCTGAACAC GGCCATCCAC 
AGTCCAGGTC GTTTCCGGGT GATTGGGACG CTCTCGAACT CCGTTGACTT TGCGCGGGAG 
TTCAATTGTC CCCTCGGCTC ACCGATGAAC CCTCAGAAAA AGTGCAGCGT TTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262773.1
CDS	387..3323
Translation	MVMLPLTLVLILIMRLDGMLAALQLNEQRMRDLRNSHSEVPVYMEDYEALLPEGSTYNDLINEEFILPASKRTQLQILAAERARRCQPYRYGNGESMELEERNTLMKDSRTSFLPLGIPRECLGSGIELDIKPIDEEAYQRQKKRYQDIAPYWLEKIRIRERREAERHAEEASAEISEATAALQSFWNEEGTREGIRMTQAKTMKRYMDNKVDPCVDFYKYACGNWERLHPIPKDKAGFDTFEMLRESLDLVLRNLLEKNTPVHSAAELRKSPVRNTLFKLNEQGEGEGEADQAAELTAERLRRHIVSKRQLLNRVLVRYKRYTNGTKRKRLIETPRERTKEEEAAPPVVLPKDKTKDKSDNEEQLHVPTDFLKPHQDAQLKAKNLYRSCVNSAVLAKRGLEPLHTLIRELGGWPVLESQWSESNFNWQVLAATLRRYNNDILIVQWVGADIKNSEENIVQFDQTGLGLPTREYFLQPSNAKYLQAYQRYMAEVMHKMGASKADAQRVASELVAFETQLAGITAPAEQRLNVTKLYKRMTLDQLQAVVPEIKWRAYLQSLQDREVLGTEEVVIYAVEYMSKLVTLLDETDPRTVSNYMMWRFVRHRINNVDDRFDDIKQSFYHALFGREESPQRWKVCIAQVNTNMGMAVGSMFVSRYFDNNSKRDTLRMTHDLQQAFRDILKTTDWLDDTTKQLAEEKVNAMSLKIGYPDFILNPSELNSKYAGIEIYPEKYFENTLNVLLHTAKTEQAKLHERVNKTNWQTAPAIVNAYYSRNKNQIMFPAGILQPPFYHRHFPKSLNFGGIGVVIGHELTHGFDDKGRLFDRNGNIHKWWTDSSIRGFDERARCIIAQYSNYTVEEVGIVLNGESTQGENIADNGGLRQAFHAYQRWLKEHPSEVSDEILPGLNMTGPQLFFLNFGQVWCGAMRPEAIRNKLNTAIHSPGRFRVIGTLSNSVDFAREFNCPLGSPMNPQKKCSVW

Target ORF information:

RefSeq Version	NM_001275844.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neprilysin 4, transcript variant C (Nep4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275844.1

ORF Insert Sequence:

ATGGTAATGC TGCCACTGAC CCTGGTGCTC ATCCTGATCA TGCGGCTGGA CGGGATGCTG 
GCGGCGCTGC AATTGAACGA ACAGAGGATG AGGGATCTGC GGAACTCTCA CAGCGAGGTG 
CCTGTCTATA TGGAGGATTA CGAAGCCCTT TTACCGGAGG GTAGTACCTA CAACGACCTG 
ATCAACGAGG AGTTCATACT GCCGGCGAGC AAGCGGACCC AACTGCAGAT TTTGGCCGCG 
GAGAGGGCGC GTCGCTGCCA ACCATATCGC TACGGGAACG GGGAGTCCAT GGAGTTGGAG 
GAGCGCAACA CGCTGATGAA GGACTCCCGA ACCTCCTTCC TGCCACTGGG CATTCCGCGA 
GAGTGCCTCG GCAGCGGCAT TGAACTGGAC ATTAAACCCA TAGATGAGGA GGCCTACCAG 
AGGCAGAAGA AGCGCTACCA GGACATAGCT CCGTATTGGC TGGAGAAGAT CAGAATACGG 
GAGCGCCGCG AGGCCGAACG TCATGCAGAG GAGGCCAGTG CCGAGATCAG CGAGGCCACC 
GCCGCTCTGC AGAGTTTCTG GAACGAGGAA GGCACTCGGG AGGGCATTCG CATGACCCAG 
GCCAAAACCA TGAAGCGCTA CATGGATAAC AAAGTGGATC CCTGCGTGGA TTTCTACAAG 
TACGCCTGCG GCAACTGGGA GCGCCTGCAT CCAATACCCA AGGATAAGGC CGGCTTCGAT 
ACCTTCGAAA TGCTGCGTGA GAGTCTGGAC CTGGTGCTAC GAAATCTTCT GGAGAAAAAT 
ACCCCTGTGC ACTCCGCAGC GGAGCTACGA AAAAGTCCCG TGCGGAATAC TCTATTTAAG 
CTTAATGAGC AGGGCGAGGG TGAGGGCGAA GCTGACCAGG CGGCGGAACT TACGGCGGAA 
CGCCTGCGTC GGCACATTGT CAGTAAGAGG CAGTTGCTTA ACCGCGTTCT GGTGCGCTAC 
AAGCGGTATA CCAACGGAAC GAAAAGGAAA CGCCTCATCG AAACCCCACG GGAGAGAACC 
AAAGAGGAGG AAGCTGCTCC ACCAGTTGTC CTGCCAAAGG ATAAAACCAA GGACAAGTCG 
GATAACGAAG AGCAACTACA TGTGCCCACC GATTTCCTAA AGCCCCATCA AGATGCCCAG 
CTAAAAGCAA AGAATCTGTA CCGGTCCTGC GTGAACAGCG CGGTGCTGGC CAAGCGGGGA 
TTGGAACCGC TGCACACGCT CATTCGGGAA CTAGGTGGCT GGCCAGTCCT GGAGTCCCAA 
TGGAGTGAGT CCAACTTTAA CTGGCAGGTG CTAGCCGCCA CCCTTAGACG TTACAACAAC 
GACATCCTCA TCGTCCAGTG GGTGGGGGCG GACATCAAGA ACTCCGAGGA GAACATTGTT 
CAGTTTGACC AGACGGGTCT GGGCCTGCCC ACCAGGGAGT ACTTCCTCCA GCCGAGCAAC 
GCCAAGTACC TACAGGCCTA CCAGCGCTAC ATGGCCGAGG TGATGCACAA AATGGGCGCC 
TCCAAGGCGG ATGCCCAGCG GGTGGCCAGC GAGCTGGTGG CGTTCGAAAC GCAGCTGGCG 
GGGATTACAG CTCCAGCGGA GCAACGACTT AACGTCACCA AGCTCTACAA GCGCATGACG 
CTGGACCAAC TGCAGGCGGT GGTTCCGGAA ATCAAGTGGC GAGCTTACCT GCAGAGTCTG 
CAAGATCGCG AAGTTCTGGG CACCGAGGAA GTGGTCATCT ACGCGGTGGA GTACATGAGC 
AAGCTGGTGA CTCTTCTAGA TGAAACGGAT CCCCGAACGG TTTCCAATTA CATGATGTGG 
CGCTTTGTGC GGCACAGGAT CAACAACGTG GACGATCGAT TCGATGACAT CAAACAGAGT 
TTCTACCACG CCCTCTTTGG CCGCGAGGAG AGTCCGCAGA GATGGAAGGT ATGCATCGCC 
CAGGTGAACA CCAACATGGG CATGGCAGTG GGTTCCATGT TCGTGAGTCG CTATTTCGAC 
AACAACAGCA AGCGGGACAC CTTGCGGATG ACTCACGATC TGCAGCAGGC CTTTCGGGAT 
ATCCTGAAAA CCACTGATTG GCTGGACGAC ACCACAAAGC AGTTGGCCGA GGAAAAGGTT 
AACGCCATGT CCCTGAAGAT CGGCTATCCA GACTTTATCC TTAATCCCAG TGAGTTAAAT 
AGCAAGTACG CGGGCATAGA AATCTATCCG GAAAAGTATT TTGAGAACAC TCTGAATGTT 
CTGCTTCACA CGGCCAAAAC GGAGCAGGCC AAGCTCCACG AGAGGGTCAA CAAGACCAAC 
TGGCAGACAG CTCCTGCCAT CGTCAATGCC TATTATAGCC GCAACAAGAA CCAGATCATG 
TTTCCCGCCG GCATCCTGCA GCCGCCCTTC TACCACCGCC ACTTTCCGAA GTCGCTGAAC 
TTCGGCGGCA TCGGTGTGGT CATTGGCCAC GAACTGACCC ACGGTTTCGA TGACAAGGGT 
AGGCTCTTCG ATCGCAACGG CAATATACAT AAGTGGTGGA CGGACTCCTC GATTCGCGGG 
TTCGACGAGC GCGCCCGCTG CATTATTGCC CAATATAGCA ACTATACCGT CGAGGAGGTG 
GGAATCGTCC TGAATGGAGA GAGTACGCAG GGTGAGAATA TCGCAGACAA TGGAGGCCTC 
CGGCAGGCCT TCCACGCGTA TCAGCGCTGG CTAAAGGAGC ATCCCAGCGA GGTGTCGGAC 
GAGATCCTGC CAGGACTTAA CATGACAGGG CCGCAGCTGT TCTTCCTGAA CTTCGGTCAG 
GTTTGGTGCG GAGCGATGCG ACCGGAGGCC ATCCGGAACA AGCTGAACAC GGCCATCCAC 
AGTCCAGGTC GTTTCCGGGT GATTGGGACG CTCTCGAACT CCGTTGACTT TGCGCGGGAG 
TTCAATTGTC CCCTCGGCTC ACCGATGAAC CCTCAGAAAA AGTGCAGCGT TTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nep4 ( NM_169912.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732540.2 Drosophila melanogaster neprilysin 4, transcript variant B (Nep4), mRNA.

Price & Availability↑

CloneID	OFa05498
Clone ID Related Accession (Same CDS sequence)	NM_001275844.1 , NM_169912.1
Accession Version	NM_169912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neprilysin 4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTAATGC TGCCACTGAC CCTGGTGCTC ATCCTGATCA TGCGGCTGGA CGGGATGCTG 
GCGGCGCTGC AATTGAACGA ACAGAGGATG AGGGATCTGC GGAACTCTCA CAGCGAGGTG 
CCTGTCTATA TGGAGGATTA CGAAGCCCTT TTACCGGAGG GTAGTACCTA CAACGACCTG 
ATCAACGAGG AGTTCATACT GCCGGCGAGC AAGCGGACCC AACTGCAGAT TTTGGCCGCG 
GAGAGGGCGC GTCGCTGCCA ACCATATCGC TACGGGAACG GGGAGTCCAT GGAGTTGGAG 
GAGCGCAACA CGCTGATGAA GGACTCCCGA ACCTCCTTCC TGCCACTGGG CATTCCGCGA 
GAGTGCCTCG GCAGCGGCAT TGAACTGGAC ATTAAACCCA TAGATGAGGA GGCCTACCAG 
AGGCAGAAGA AGCGCTACCA GGACATAGCT CCGTATTGGC TGGAGAAGAT CAGAATACGG 
GAGCGCCGCG AGGCCGAACG TCATGCAGAG GAGGCCAGTG CCGAGATCAG CGAGGCCACC 
GCCGCTCTGC AGAGTTTCTG GAACGAGGAA GGCACTCGGG AGGGCATTCG CATGACCCAG 
GCCAAAACCA TGAAGCGCTA CATGGATAAC AAAGTGGATC CCTGCGTGGA TTTCTACAAG 
TACGCCTGCG GCAACTGGGA GCGCCTGCAT CCAATACCCA AGGATAAGGC CGGCTTCGAT 
ACCTTCGAAA TGCTGCGTGA GAGTCTGGAC CTGGTGCTAC GAAATCTTCT GGAGAAAAAT 
ACCCCTGTGC ACTCCGCAGC GGAGCTACGA AAAAGTCCCG TGCGGAATAC TCTATTTAAG 
CTTAATGAGC AGGGCGAGGG TGAGGGCGAA GCTGACCAGG CGGCGGAACT TACGGCGGAA 
CGCCTGCGTC GGCACATTGT CAGTAAGAGG CAGTTGCTTA ACCGCGTTCT GGTGCGCTAC 
AAGCGGTATA CCAACGGAAC GAAAAGGAAA CGCCTCATCG AAACCCCACG GGAGAGAACC 
AAAGAGGAGG AAGCTGCTCC ACCAGTTGTC CTGCCAAAGG ATAAAACCAA GGACAAGTCG 
GATAACGAAG AGCAACTACA TGTGCCCACC GATTTCCTAA AGCCCCATCA AGATGCCCAG 
CTAAAAGCAA AGAATCTGTA CCGGTCCTGC GTGAACAGCG CGGTGCTGGC CAAGCGGGGA 
TTGGAACCGC TGCACACGCT CATTCGGGAA CTAGGTGGCT GGCCAGTCCT GGAGTCCCAA 
TGGAGTGAGT CCAACTTTAA CTGGCAGGTG CTAGCCGCCA CCCTTAGACG TTACAACAAC 
GACATCCTCA TCGTCCAGTG GGTGGGGGCG GACATCAAGA ACTCCGAGGA GAACATTGTT 
CAGTTTGACC AGACGGGTCT GGGCCTGCCC ACCAGGGAGT ACTTCCTCCA GCCGAGCAAC 
GCCAAGTACC TACAGGCCTA CCAGCGCTAC ATGGCCGAGG TGATGCACAA AATGGGCGCC 
TCCAAGGCGG ATGCCCAGCG GGTGGCCAGC GAGCTGGTGG CGTTCGAAAC GCAGCTGGCG 
GGGATTACAG CTCCAGCGGA GCAACGACTT AACGTCACCA AGCTCTACAA GCGCATGACG 
CTGGACCAAC TGCAGGCGGT GGTTCCGGAA ATCAAGTGGC GAGCTTACCT GCAGAGTCTG 
CAAGATCGCG AAGTTCTGGG CACCGAGGAA GTGGTCATCT ACGCGGTGGA GTACATGAGC 
AAGCTGGTGA CTCTTCTAGA TGAAACGGAT CCCCGAACGG TTTCCAATTA CATGATGTGG 
CGCTTTGTGC GGCACAGGAT CAACAACGTG GACGATCGAT TCGATGACAT CAAACAGAGT 
TTCTACCACG CCCTCTTTGG CCGCGAGGAG AGTCCGCAGA GATGGAAGGT ATGCATCGCC 
CAGGTGAACA CCAACATGGG CATGGCAGTG GGTTCCATGT TCGTGAGTCG CTATTTCGAC 
AACAACAGCA AGCGGGACAC CTTGCGGATG ACTCACGATC TGCAGCAGGC CTTTCGGGAT 
ATCCTGAAAA CCACTGATTG GCTGGACGAC ACCACAAAGC AGTTGGCCGA GGAAAAGGTT 
AACGCCATGT CCCTGAAGAT CGGCTATCCA GACTTTATCC TTAATCCCAG TGAGTTAAAT 
AGCAAGTACG CGGGCATAGA AATCTATCCG GAAAAGTATT TTGAGAACAC TCTGAATGTT 
CTGCTTCACA CGGCCAAAAC GGAGCAGGCC AAGCTCCACG AGAGGGTCAA CAAGACCAAC 
TGGCAGACAG CTCCTGCCAT CGTCAATGCC TATTATAGCC GCAACAAGAA CCAGATCATG 
TTTCCCGCCG GCATCCTGCA GCCGCCCTTC TACCACCGCC ACTTTCCGAA GTCGCTGAAC 
TTCGGCGGCA TCGGTGTGGT CATTGGCCAC GAACTGACCC ACGGTTTCGA TGACAAGGGT 
AGGCTCTTCG ATCGCAACGG CAATATACAT AAGTGGTGGA CGGACTCCTC GATTCGCGGG 
TTCGACGAGC GCGCCCGCTG CATTATTGCC CAATATAGCA ACTATACCGT CGAGGAGGTG 
GGAATCGTCC TGAATGGAGA GAGTACGCAG GGTGAGAATA TCGCAGACAA TGGAGGCCTC 
CGGCAGGCCT TCCACGCGTA TCAGCGCTGG CTAAAGGAGC ATCCCAGCGA GGTGTCGGAC 
GAGATCCTGC CAGGACTTAA CATGACAGGG CCGCAGCTGT TCTTCCTGAA CTTCGGTCAG 
GTTTGGTGCG GAGCGATGCG ACCGGAGGCC ATCCGGAACA AGCTGAACAC GGCCATCCAC 
AGTCCAGGTC GTTTCCGGGT GATTGGGACG CTCTCGAACT CCGTTGACTT TGCGCGGGAG 
TTCAATTGTC CCCTCGGCTC ACCGATGAAC CCTCAGAAAA AGTGCAGCGT TTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732540.2
CDS	89..3025
Translation	MVMLPLTLVLILIMRLDGMLAALQLNEQRMRDLRNSHSEVPVYMEDYEALLPEGSTYNDLINEEFILPASKRTQLQILAAERARRCQPYRYGNGESMELEERNTLMKDSRTSFLPLGIPRECLGSGIELDIKPIDEEAYQRQKKRYQDIAPYWLEKIRIRERREAERHAEEASAEISEATAALQSFWNEEGTREGIRMTQAKTMKRYMDNKVDPCVDFYKYACGNWERLHPIPKDKAGFDTFEMLRESLDLVLRNLLEKNTPVHSAAELRKSPVRNTLFKLNEQGEGEGEADQAAELTAERLRRHIVSKRQLLNRVLVRYKRYTNGTKRKRLIETPRERTKEEEAAPPVVLPKDKTKDKSDNEEQLHVPTDFLKPHQDAQLKAKNLYRSCVNSAVLAKRGLEPLHTLIRELGGWPVLESQWSESNFNWQVLAATLRRYNNDILIVQWVGADIKNSEENIVQFDQTGLGLPTREYFLQPSNAKYLQAYQRYMAEVMHKMGASKADAQRVASELVAFETQLAGITAPAEQRLNVTKLYKRMTLDQLQAVVPEIKWRAYLQSLQDREVLGTEEVVIYAVEYMSKLVTLLDETDPRTVSNYMMWRFVRHRINNVDDRFDDIKQSFYHALFGREESPQRWKVCIAQVNTNMGMAVGSMFVSRYFDNNSKRDTLRMTHDLQQAFRDILKTTDWLDDTTKQLAEEKVNAMSLKIGYPDFILNPSELNSKYAGIEIYPEKYFENTLNVLLHTAKTEQAKLHERVNKTNWQTAPAIVNAYYSRNKNQIMFPAGILQPPFYHRHFPKSLNFGGIGVVIGHELTHGFDDKGRLFDRNGNIHKWWTDSSIRGFDERARCIIAQYSNYTVEEVGIVLNGESTQGENIADNGGLRQAFHAYQRWLKEHPSEVSDEILPGLNMTGPQLFFLNFGQVWCGAMRPEAIRNKLNTAIHSPGRFRVIGTLSNSVDFAREFNCPLGSPMNPQKKCSVW

Target ORF information:

RefSeq Version	NM_169912.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neprilysin 4, transcript variant B (Nep4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169912.1

ORF Insert Sequence:

ATGGTAATGC TGCCACTGAC CCTGGTGCTC ATCCTGATCA TGCGGCTGGA CGGGATGCTG 
GCGGCGCTGC AATTGAACGA ACAGAGGATG AGGGATCTGC GGAACTCTCA CAGCGAGGTG 
CCTGTCTATA TGGAGGATTA CGAAGCCCTT TTACCGGAGG GTAGTACCTA CAACGACCTG 
ATCAACGAGG AGTTCATACT GCCGGCGAGC AAGCGGACCC AACTGCAGAT TTTGGCCGCG 
GAGAGGGCGC GTCGCTGCCA ACCATATCGC TACGGGAACG GGGAGTCCAT GGAGTTGGAG 
GAGCGCAACA CGCTGATGAA GGACTCCCGA ACCTCCTTCC TGCCACTGGG CATTCCGCGA 
GAGTGCCTCG GCAGCGGCAT TGAACTGGAC ATTAAACCCA TAGATGAGGA GGCCTACCAG 
AGGCAGAAGA AGCGCTACCA GGACATAGCT CCGTATTGGC TGGAGAAGAT CAGAATACGG 
GAGCGCCGCG AGGCCGAACG TCATGCAGAG GAGGCCAGTG CCGAGATCAG CGAGGCCACC 
GCCGCTCTGC AGAGTTTCTG GAACGAGGAA GGCACTCGGG AGGGCATTCG CATGACCCAG 
GCCAAAACCA TGAAGCGCTA CATGGATAAC AAAGTGGATC CCTGCGTGGA TTTCTACAAG 
TACGCCTGCG GCAACTGGGA GCGCCTGCAT CCAATACCCA AGGATAAGGC CGGCTTCGAT 
ACCTTCGAAA TGCTGCGTGA GAGTCTGGAC CTGGTGCTAC GAAATCTTCT GGAGAAAAAT 
ACCCCTGTGC ACTCCGCAGC GGAGCTACGA AAAAGTCCCG TGCGGAATAC TCTATTTAAG 
CTTAATGAGC AGGGCGAGGG TGAGGGCGAA GCTGACCAGG CGGCGGAACT TACGGCGGAA 
CGCCTGCGTC GGCACATTGT CAGTAAGAGG CAGTTGCTTA ACCGCGTTCT GGTGCGCTAC 
AAGCGGTATA CCAACGGAAC GAAAAGGAAA CGCCTCATCG AAACCCCACG GGAGAGAACC 
AAAGAGGAGG AAGCTGCTCC ACCAGTTGTC CTGCCAAAGG ATAAAACCAA GGACAAGTCG 
GATAACGAAG AGCAACTACA TGTGCCCACC GATTTCCTAA AGCCCCATCA AGATGCCCAG 
CTAAAAGCAA AGAATCTGTA CCGGTCCTGC GTGAACAGCG CGGTGCTGGC CAAGCGGGGA 
TTGGAACCGC TGCACACGCT CATTCGGGAA CTAGGTGGCT GGCCAGTCCT GGAGTCCCAA 
TGGAGTGAGT CCAACTTTAA CTGGCAGGTG CTAGCCGCCA CCCTTAGACG TTACAACAAC 
GACATCCTCA TCGTCCAGTG GGTGGGGGCG GACATCAAGA ACTCCGAGGA GAACATTGTT 
CAGTTTGACC AGACGGGTCT GGGCCTGCCC ACCAGGGAGT ACTTCCTCCA GCCGAGCAAC 
GCCAAGTACC TACAGGCCTA CCAGCGCTAC ATGGCCGAGG TGATGCACAA AATGGGCGCC 
TCCAAGGCGG ATGCCCAGCG GGTGGCCAGC GAGCTGGTGG CGTTCGAAAC GCAGCTGGCG 
GGGATTACAG CTCCAGCGGA GCAACGACTT AACGTCACCA AGCTCTACAA GCGCATGACG 
CTGGACCAAC TGCAGGCGGT GGTTCCGGAA ATCAAGTGGC GAGCTTACCT GCAGAGTCTG 
CAAGATCGCG AAGTTCTGGG CACCGAGGAA GTGGTCATCT ACGCGGTGGA GTACATGAGC 
AAGCTGGTGA CTCTTCTAGA TGAAACGGAT CCCCGAACGG TTTCCAATTA CATGATGTGG 
CGCTTTGTGC GGCACAGGAT CAACAACGTG GACGATCGAT TCGATGACAT CAAACAGAGT 
TTCTACCACG CCCTCTTTGG CCGCGAGGAG AGTCCGCAGA GATGGAAGGT ATGCATCGCC 
CAGGTGAACA CCAACATGGG CATGGCAGTG GGTTCCATGT TCGTGAGTCG CTATTTCGAC 
AACAACAGCA AGCGGGACAC CTTGCGGATG ACTCACGATC TGCAGCAGGC CTTTCGGGAT 
ATCCTGAAAA CCACTGATTG GCTGGACGAC ACCACAAAGC AGTTGGCCGA GGAAAAGGTT 
AACGCCATGT CCCTGAAGAT CGGCTATCCA GACTTTATCC TTAATCCCAG TGAGTTAAAT 
AGCAAGTACG CGGGCATAGA AATCTATCCG GAAAAGTATT TTGAGAACAC TCTGAATGTT 
CTGCTTCACA CGGCCAAAAC GGAGCAGGCC AAGCTCCACG AGAGGGTCAA CAAGACCAAC 
TGGCAGACAG CTCCTGCCAT CGTCAATGCC TATTATAGCC GCAACAAGAA CCAGATCATG 
TTTCCCGCCG GCATCCTGCA GCCGCCCTTC TACCACCGCC ACTTTCCGAA GTCGCTGAAC 
TTCGGCGGCA TCGGTGTGGT CATTGGCCAC GAACTGACCC ACGGTTTCGA TGACAAGGGT 
AGGCTCTTCG ATCGCAACGG CAATATACAT AAGTGGTGGA CGGACTCCTC GATTCGCGGG 
TTCGACGAGC GCGCCCGCTG CATTATTGCC CAATATAGCA ACTATACCGT CGAGGAGGTG 
GGAATCGTCC TGAATGGAGA GAGTACGCAG GGTGAGAATA TCGCAGACAA TGGAGGCCTC 
CGGCAGGCCT TCCACGCGTA TCAGCGCTGG CTAAAGGAGC ATCCCAGCGA GGTGTCGGAC 
GAGATCCTGC CAGGACTTAA CATGACAGGG CCGCAGCTGT TCTTCCTGAA CTTCGGTCAG 
GTTTGGTGCG GAGCGATGCG ACCGGAGGCC ATCCGGAACA AGCTGAACAC GGCCATCCAC 
AGTCCAGGTC GTTTCCGGGT GATTGGGACG CTCTCGAACT CCGTTGACTT TGCGCGGGAG 
TTCAATTGTC CCCTCGGCTC ACCGATGAAC CCTCAGAAAA AGTGCAGCGT TTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nep4 ( NM_142647.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650904.3 Drosophila melanogaster neprilysin 4, transcript variant A (Nep4), mRNA.

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CloneID	OFa05499
Clone ID Related Accession (Same CDS sequence)	NM_142647.3
Accession Version	NM_142647.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neprilysin 4, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142647.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGCC ACAGCCAACT GAAGCTAGCG ATGCCCTCGG TTCACGGAGC TCCAGCCACC 
GCTCCTGGCT CGCCGATGAA CGCCAAAGCC CGGAGCGTGA AGCTCGGACT GGGTGTTAAT 
CAGCGGACAG GTCGTGTGCA GTGGTGTCCC GGTCTGACCT GCTGCAAAAT GCTTCTACTT 
CTTCCAGTGG TAATGCTGCC ACTGACCCTG GTGCTCATCC TGATCATGCG GCTGGACGGG 
ATGCTGGCGG CGCTGCAATT GAACGAACAG AGGATGAGGG ATCTGCGGAA CTCTCACAGC 
GAGGTGCCTG TCTATATGGA GGATTACGAA GCCCTTTTAC CGGAGGGTAG TACCTACAAC 
GACCTGATCA ACGAGGAGTT CATACTGCCG GCGAGCAAGC GGACCCAACT GCAGATTTTG 
GCCGCGGAGA GGGCGCGTCG CTGCCAACCA TATCGCTACG GGAACGGGGA GTCCATGGAG 
TTGGAGGAGC GCAACACGCT GATGAAGGAC TCCCGAACCT CCTTCCTGCC ACTGGGCATT 
CCGCGAGAGT GCCTCGGCAG CGGCATTGAA CTGGACATTA AACCCATAGA TGAGGAGGCC 
TACCAGAGGC AGAAGAAGCG CTACCAGGAC ATAGCTCCGT ATTGGCTGGA GAAGATCAGA 
ATACGGGAGC GCCGCGAGGC CGAACGTCAT GCAGAGGAGG CCAGTGCCGA GATCAGCGAG 
GCCACCGCCG CTCTGCAGAG TTTCTGGAAC GAGGAAGGCA CTCGGGAGGG CATTCGCATG 
ACCCAGGCCA AAACCATGAA GCGCTACATG GATAACAAAG TGGATCCCTG CGTGGATTTC 
TACAAGTACG CCTGCGGCAA CTGGGAGCGC CTGCATCCAA TACCCAAGGA TAAGGCCGGC 
TTCGATACCT TCGAAATGCT GCGTGAGAGT CTGGACCTGG TGCTACGAAA TCTTCTGGAG 
AAAAATACCC CTGTGCACTC CGCAGCGGAG CTACGAAAAA GTCCCGTGCG GAATACTCTA 
TTTAAGCTTA ATGAGCAGGG CGAGGGTGAG GGCGAAGCTG ACCAGGCGGC GGAACTTACG 
GCGGAACGCC TGCGTCGGCA CATTGTCAGT AAGAGGCAGT TGCTTAACCG CGTTCTGGTG 
CGCTACAAGC GGTATACCAA CGGAACGAAA AGGAAACGCC TCATCGAAAC CCCACGGGAG 
AGAACCAAAG AGGAGGAAGC TGCTCCACCA GTTGTCCTGC CAAAGGATAA AACCAAGGAC 
AAGTCGGATA ACGAAGAGCA ACTACATGTG CCCACCGATT TCCTAAAGCC CCATCAAGAT 
GCCCAGCTAA AAGCAAAGAA TCTGTACCGG TCCTGCGTGA ACAGCGCGGT GCTGGCCAAG 
CGGGGATTGG AACCGCTGCA CACGCTCATT CGGGAACTAG GTGGCTGGCC AGTCCTGGAG 
TCCCAATGGA GTGAGTCCAA CTTTAACTGG CAGGTGCTAG CCGCCACCCT TAGACGTTAC 
AACAACGACA TCCTCATCGT CCAGTGGGTG GGGGCGGACA TCAAGAACTC CGAGGAGAAC 
ATTGTTCAGT TTGACCAGAC GGGTCTGGGC CTGCCCACCA GGGAGTACTT CCTCCAGCCG 
AGCAACGCCA AGTACCTACA GGCCTACCAG CGCTACATGG CCGAGGTGAT GCACAAAATG 
GGCGCCTCCA AGGCGGATGC CCAGCGGGTG GCCAGCGAGC TGGTGGCGTT CGAAACGCAG 
CTGGCGGGGA TTACAGCTCC AGCGGAGCAA CGACTTAACG TCACCAAGCT CTACAAGCGC 
ATGACGCTGG ACCAACTGCA GGCGGTGGTT CCGGAAATCA AGTGGCGAGC TTACCTGCAG 
AGTCTGCAAG ATCGCGAAGT TCTGGGCACC GAGGAAGTGG TCATCTACGC GGTGGAGTAC 
ATGAGCAAGC TGGTGACTCT TCTAGATGAA ACGGATCCCC GAACGGTTTC CAATTACATG 
ATGTGGCGCT TTGTGCGGCA CAGGATCAAC AACGTGGACG ATCGATTCGA TGACATCAAA 
CAGAGTTTCT ACCACGCCCT CTTTGGCCGC GAGGAGAGTC CGCAGAGATG GAAGGTATGC 
ATCGCCCAGG TGAACACCAA CATGGGCATG GCAGTGGGTT CCATGTTCGT GAGTCGCTAT 
TTCGACAACA ACAGCAAGCG GGACACCTTG CGGATGACTC ACGATCTGCA GCAGGCCTTT 
CGGGATATCC TGAAAACCAC TGATTGGCTG GACGACACCA CAAAGCAGTT GGCCGAGGAA 
AAGGTTAACG CCATGTCCCT GAAGATCGGC TATCCAGACT TTATCCTTAA TCCCAGTGAG 
TTAAATAGCA AGTACGCGGG CATAGAAATC TATCCGGAAA AGTATTTTGA GAACACTCTG 
AATGTTCTGC TTCACACGGC CAAAACGGAG CAGGCCAAGC TCCACGAGAG GGTCAACAAG 
ACCAACTGGC AGACAGCTCC TGCCATCGTC AATGCCTATT ATAGCCGCAA CAAGAACCAG 
ATCATGTTTC CCGCCGGCAT CCTGCAGCCG CCCTTCTACC ACCGCCACTT TCCGAAGTCG 
CTGAACTTCG GCGGCATCGG TGTGGTCATT GGCCACGAAC TGACCCACGG TTTCGATGAC 
AAGGGTAGGC TCTTCGATCG CAACGGCAAT ATACATAAGT GGTGGACGGA CTCCTCGATT 
CGCGGGTTCG ACGAGCGCGC CCGCTGCATT ATTGCCCAAT ATAGCAACTA TACCGTCGAG 
GAGGTGGGAA TCGTCCTGAA TGGAGAGAGT ACGCAGGGTG AGAATATCGC AGACAATGGA 
GGCCTCCGGC AGGCCTTCCA CGCGTATCAG CGCTGGCTAA AGGAGCATCC CAGCGAGGTG 
TCGGACGAGA TCCTGCCAGG ACTTAACATG ACAGGGCCGC AGCTGTTCTT CCTGAACTTC 
GGTCAGGTTT GGTGCGGAGC GATGCGACCG GAGGCCATCC GGAACAAGCT GAACACGGCC 
ATCCACAGTC CAGGTCGTTT CCGGGTGATT GGGACGCTCT CGAACTCCGT TGACTTTGCG 
CGGGAGTTCA ATTGTCCCCT CGGCTCACCG ATGAACCCTC AGAAAAAGTG CAGCGTTTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650904.3
CDS	204..3326
Translation	MSRHSQLKLAMPSVHGAPATAPGSPMNAKARSVKLGLGVNQRTGRVQWCPGLTCCKMLLLLPVVMLPLTLVLILIMRLDGMLAALQLNEQRMRDLRNSHSEVPVYMEDYEALLPEGSTYNDLINEEFILPASKRTQLQILAAERARRCQPYRYGNGESMELEERNTLMKDSRTSFLPLGIPRECLGSGIELDIKPIDEEAYQRQKKRYQDIAPYWLEKIRIRERREAERHAEEASAEISEATAALQSFWNEEGTREGIRMTQAKTMKRYMDNKVDPCVDFYKYACGNWERLHPIPKDKAGFDTFEMLRESLDLVLRNLLEKNTPVHSAAELRKSPVRNTLFKLNEQGEGEGEADQAAELTAERLRRHIVSKRQLLNRVLVRYKRYTNGTKRKRLIETPRERTKEEEAAPPVVLPKDKTKDKSDNEEQLHVPTDFLKPHQDAQLKAKNLYRSCVNSAVLAKRGLEPLHTLIRELGGWPVLESQWSESNFNWQVLAATLRRYNNDILIVQWVGADIKNSEENIVQFDQTGLGLPTREYFLQPSNAKYLQAYQRYMAEVMHKMGASKADAQRVASELVAFETQLAGITAPAEQRLNVTKLYKRMTLDQLQAVVPEIKWRAYLQSLQDREVLGTEEVVIYAVEYMSKLVTLLDETDPRTVSNYMMWRFVRHRINNVDDRFDDIKQSFYHALFGREESPQRWKVCIAQVNTNMGMAVGSMFVSRYFDNNSKRDTLRMTHDLQQAFRDILKTTDWLDDTTKQLAEEKVNAMSLKIGYPDFILNPSELNSKYAGIEIYPEKYFENTLNVLLHTAKTEQAKLHERVNKTNWQTAPAIVNAYYSRNKNQIMFPAGILQPPFYHRHFPKSLNFGGIGVVIGHELTHGFDDKGRLFDRNGNIHKWWTDSSIRGFDERARCIIAQYSNYTVEEVGIVLNGESTQGENIADNGGLRQAFHAYQRWLKEHPSEVSDEILPGLNMTGPQLFFLNFGQVWCGAMRPEAIRNKLNTAIHSPGRFRVIGTLSNSVDFAREFNCPLGSPMNPQKKCSVW

Target ORF information:

RefSeq Version	NM_142647.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neprilysin 4, transcript variant A (Nep4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142647.3

ORF Insert Sequence:

ATGAGTCGCC ACAGCCAACT GAAGCTAGCG ATGCCCTCGG TTCACGGAGC TCCAGCCACC 
GCTCCTGGCT CGCCGATGAA CGCCAAAGCC CGGAGCGTGA AGCTCGGACT GGGTGTTAAT 
CAGCGGACAG GTCGTGTGCA GTGGTGTCCC GGTCTGACCT GCTGCAAAAT GCTTCTACTT 
CTTCCAGTGG TAATGCTGCC ACTGACCCTG GTGCTCATCC TGATCATGCG GCTGGACGGG 
ATGCTGGCGG CGCTGCAATT GAACGAACAG AGGATGAGGG ATCTGCGGAA CTCTCACAGC 
GAGGTGCCTG TCTATATGGA GGATTACGAA GCCCTTTTAC CGGAGGGTAG TACCTACAAC 
GACCTGATCA ACGAGGAGTT CATACTGCCG GCGAGCAAGC GGACCCAACT GCAGATTTTG 
GCCGCGGAGA GGGCGCGTCG CTGCCAACCA TATCGCTACG GGAACGGGGA GTCCATGGAG 
TTGGAGGAGC GCAACACGCT GATGAAGGAC TCCCGAACCT CCTTCCTGCC ACTGGGCATT 
CCGCGAGAGT GCCTCGGCAG CGGCATTGAA CTGGACATTA AACCCATAGA TGAGGAGGCC 
TACCAGAGGC AGAAGAAGCG CTACCAGGAC ATAGCTCCGT ATTGGCTGGA GAAGATCAGA 
ATACGGGAGC GCCGCGAGGC CGAACGTCAT GCAGAGGAGG CCAGTGCCGA GATCAGCGAG 
GCCACCGCCG CTCTGCAGAG TTTCTGGAAC GAGGAAGGCA CTCGGGAGGG CATTCGCATG 
ACCCAGGCCA AAACCATGAA GCGCTACATG GATAACAAAG TGGATCCCTG CGTGGATTTC 
TACAAGTACG CCTGCGGCAA CTGGGAGCGC CTGCATCCAA TACCCAAGGA TAAGGCCGGC 
TTCGATACCT TCGAAATGCT GCGTGAGAGT CTGGACCTGG TGCTACGAAA TCTTCTGGAG 
AAAAATACCC CTGTGCACTC CGCAGCGGAG CTACGAAAAA GTCCCGTGCG GAATACTCTA 
TTTAAGCTTA ATGAGCAGGG CGAGGGTGAG GGCGAAGCTG ACCAGGCGGC GGAACTTACG 
GCGGAACGCC TGCGTCGGCA CATTGTCAGT AAGAGGCAGT TGCTTAACCG CGTTCTGGTG 
CGCTACAAGC GGTATACCAA CGGAACGAAA AGGAAACGCC TCATCGAAAC CCCACGGGAG 
AGAACCAAAG AGGAGGAAGC TGCTCCACCA GTTGTCCTGC CAAAGGATAA AACCAAGGAC 
AAGTCGGATA ACGAAGAGCA ACTACATGTG CCCACCGATT TCCTAAAGCC CCATCAAGAT 
GCCCAGCTAA AAGCAAAGAA TCTGTACCGG TCCTGCGTGA ACAGCGCGGT GCTGGCCAAG 
CGGGGATTGG AACCGCTGCA CACGCTCATT CGGGAACTAG GTGGCTGGCC AGTCCTGGAG 
TCCCAATGGA GTGAGTCCAA CTTTAACTGG CAGGTGCTAG CCGCCACCCT TAGACGTTAC 
AACAACGACA TCCTCATCGT CCAGTGGGTG GGGGCGGACA TCAAGAACTC CGAGGAGAAC 
ATTGTTCAGT TTGACCAGAC GGGTCTGGGC CTGCCCACCA GGGAGTACTT CCTCCAGCCG 
AGCAACGCCA AGTACCTACA GGCCTACCAG CGCTACATGG CCGAGGTGAT GCACAAAATG 
GGCGCCTCCA AGGCGGATGC CCAGCGGGTG GCCAGCGAGC TGGTGGCGTT CGAAACGCAG 
CTGGCGGGGA TTACAGCTCC AGCGGAGCAA CGACTTAACG TCACCAAGCT CTACAAGCGC 
ATGACGCTGG ACCAACTGCA GGCGGTGGTT CCGGAAATCA AGTGGCGAGC TTACCTGCAG 
AGTCTGCAAG ATCGCGAAGT TCTGGGCACC GAGGAAGTGG TCATCTACGC GGTGGAGTAC 
ATGAGCAAGC TGGTGACTCT TCTAGATGAA ACGGATCCCC GAACGGTTTC CAATTACATG 
ATGTGGCGCT TTGTGCGGCA CAGGATCAAC AACGTGGACG ATCGATTCGA TGACATCAAA 
CAGAGTTTCT ACCACGCCCT CTTTGGCCGC GAGGAGAGTC CGCAGAGATG GAAGGTATGC 
ATCGCCCAGG TGAACACCAA CATGGGCATG GCAGTGGGTT CCATGTTCGT GAGTCGCTAT 
TTCGACAACA ACAGCAAGCG GGACACCTTG CGGATGACTC ACGATCTGCA GCAGGCCTTT 
CGGGATATCC TGAAAACCAC TGATTGGCTG GACGACACCA CAAAGCAGTT GGCCGAGGAA 
AAGGTTAACG CCATGTCCCT GAAGATCGGC TATCCAGACT TTATCCTTAA TCCCAGTGAG 
TTAAATAGCA AGTACGCGGG CATAGAAATC TATCCGGAAA AGTATTTTGA GAACACTCTG 
AATGTTCTGC TTCACACGGC CAAAACGGAG CAGGCCAAGC TCCACGAGAG GGTCAACAAG 
ACCAACTGGC AGACAGCTCC TGCCATCGTC AATGCCTATT ATAGCCGCAA CAAGAACCAG 
ATCATGTTTC CCGCCGGCAT CCTGCAGCCG CCCTTCTACC ACCGCCACTT TCCGAAGTCG 
CTGAACTTCG GCGGCATCGG TGTGGTCATT GGCCACGAAC TGACCCACGG TTTCGATGAC 
AAGGGTAGGC TCTTCGATCG CAACGGCAAT ATACATAAGT GGTGGACGGA CTCCTCGATT 
CGCGGGTTCG ACGAGCGCGC CCGCTGCATT ATTGCCCAAT ATAGCAACTA TACCGTCGAG 
GAGGTGGGAA TCGTCCTGAA TGGAGAGAGT ACGCAGGGTG AGAATATCGC AGACAATGGA 
GGCCTCCGGC AGGCCTTCCA CGCGTATCAG CGCTGGCTAA AGGAGCATCC CAGCGAGGTG 
TCGGACGAGA TCCTGCCAGG ACTTAACATG ACAGGGCCGC AGCTGTTCTT CCTGAACTTC 
GGTCAGGTTT GGTGCGGAGC GATGCGACCG GAGGCCATCC GGAACAAGCT GAACACGGCC 
ATCCACAGTC CAGGTCGTTT CCGGGTGATT GGGACGCTCT CGAACTCCGT TGACTTTGCG 
CGGGAGTTCA ATTGTCCCCT CGGCTCACCG ATGAACCCTC AGAAAAAGTG CAGCGTTTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nep4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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