DAGLA cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	DAGLA
Entrez Gene ID	269060
Full Name	diacylglycerol lipase, alpha
Synonyms	Nsddr
General protein information	Preferred Names diacylglycerol lipase, alpha Names sn1-specific diacylglycerol lipase alpha DGL-alpha neural stem cell-derived dendrite regulator
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
XM_017318213.1	XP_017173702.1	sn1-specific diacylglycerol lipase alpha isoform X1
NM_198114.2	NP_932782.2	sn1-specific diacylglycerol lipase alpha
XM_017318213.2	XP_017173702.1	sn1-specific diacylglycerol lipase alpha isoform X1
XM_006527114.3	XP_006527177.1	sn1-specific diacylglycerol lipase alpha isoform X1
XM_006527114.2	XP_006527177.1	sn1-specific diacylglycerol lipase alpha isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	dagla	XP_697873.5
Homo sapiens (human)	DAGLA	NP_006124.1
Canis lupus familiaris (dog)	DAGLA	XP_005631813.1
Mus musculus (house mouse)	Dagla	NP_932782.2
Gallus gallus (chicken)	DAGLA	XP_423696.3
Xenopus tropicalis (tropical clawed frog)	dagla	XP_004913466.1
Rattus norvegicus (Norway rat)	Dagla	NP_001005886.1
Pan troglodytes (chimpanzee)	DAGLA	XP_508479.4
Bos taurus (cattle)	DAGLA	NP_001179512.1

Gene Ontology
Bibliography

Related articles in PubMed

Crucial Roles of the Endocannabinoid 2-Arachidonoylglycerol in the Suppression of Epileptic Seizures.
Sugaya Y, Yamazaki M, Uchigashima M, Kobayashi K, Watanabe M, Sakimura K, Kano M
Cell reports16(5)1405-1415(2016 08)

Myeloid-Specific Deletion of Diacylglycerol Lipase ? Inhibits Atherogenesis in ApoE-Deficient Mice.
Jehle J, Hoyer FF, Sch?ne B, Pfeifer P, Schild K, Jenniches I, Bindila L, Lutz B, L?tjohann D, Zimmer A, Nickenig G
PloS one11(1)e0146267(2016)

Rapid and profound rewiring of brain lipid signaling networks by acute diacylglycerol lipase inhibition.
Ogasawara D, Deng H, Viader A, Baggelaar MP, Breman A, den Dulk H, van den Nieuwendijk AM, van den Nieuwendijk AM, Soethoudt M, van der Wel T, Zhou J, Overkleeft HS, Sanchez-Alavez M, Mori S, Mo S, Nguyen W, Conti B, Liu X, Chen Y, Liu QS, Cravatt BF, van der Stelt M
Proceedings of the National Academy of Sciences of the United States of America113(1)26-33(2016 Jan)

A chemical proteomic atlas of brain serine hydrolases identifies cell type-specific pathways regulating neuroinflammation.
Viader A, Ogasawara D, Joslyn CM, Sanchez-Alavez M, Mori S, Nguyen W, Conti B, Cravatt BF
eLife5e12345(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrate that mice with a deletion of the DAGL-alpha enzyme show a phenotype of increased emotional or stress-related behaviors. This is characterized by a reduced exploration of the central area of the open field, a maternal neglect behavior, a fear extinction deficit, increased behavioral despair, increased anxiety-related behaviors in the light/dark box, and reduced hippocampal neurogenesis
Title: Anxiety, Stress, and Fear Response in Mice With Reduced Endocannabinoid Levels.

Authors demonstrated that phospholipase D and diacylglycerol lipase were required for providing arachidonic acid for prostaglandin E(2) biosynthesis.
Title: Francisella tularensis LVS induction of prostaglandin biosynthesis by infected macrophages requires specific host phospholipases and lipid phosphatases.

In corpus striatum diacylglycerol lipase-alpha activity is regulated by calcium/calmodulin dependent protein kinase (CaMK-II).
Title: CaMKII regulates diacylglycerol lipase-? and striatal endocannabinoid signaling.

DAGLalpha is the major biosynthetic enzyme for 2-AG in the nervous system and reveal an essential role for this enzyme in regulating retrograde synaptic plasticity and adult neurogenesis.
Title: Loss of retrograde endocannabinoid signaling and reduced adult neurogenesis in diacylglycerol lipase knock-out mice.

Endocannabinoid 2-arachidonoylglycerol produced by DGLalpha, but not by DGLbeta, mediates retrograde suppression at central synapses.
Title: The endocannabinoid 2-arachidonoylglycerol produced by diacylglycerol lipase alpha mediates retrograde suppression of synaptic transmission.

The following DAGLA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAGLA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20995	NM_198114.2 Latest version!	Mus musculus diacylglycerol lipase, alpha (Dagla), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu20995	XM_006527114.3 Latest version!	Mus musculus diacylglycerol lipase, alpha (Dagla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu20995	XM_017318213.2 Latest version!	Mus musculus diacylglycerol lipase, alpha (Dagla), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu20995	XM_017318213.1	Mus musculus diacylglycerol lipase, alpha (Dagla), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu20995	XM_006527114.2	Mus musculus diacylglycerol lipase, alpha (Dagla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DAGLA ( NM_198114.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_932782.2 Mus musculus diacylglycerol lipase, alpha (Dagla), mRNA.

Price & Availability↑

CloneID	OMu20995
Clone ID Related Accession (Same CDS sequence)	XM_017318213.1 , NM_198114.2 , XM_017318213.2 , XM_006527114.3 , XM_006527114.2
Accession Version	NM_198114.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Mus musculus(house mouse)
Product	sn1-specific diacylglycerol lipase alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ127451.1, AK147384.1, BC112414.1, CF539357.1 and BP765314.1. On Jul 27, 2007 this sequence version replaced NM_198114.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1660815.129317.1, BC148308.2 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCTATCT TCCTCTTCCT GCTACACACC ACCTGGTTTG TAATCCTGTC TGTGGTGCTC 
TTCGGCTTGG TCTATAACCC ACACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGA 
GGCTACCTGG GCATCCTTCT GAGCTGTATG ATTGCCGAAA TGGCTATCAT CTGGCTGAGC 
ATGCGTGGGG GCATCCTCTA CACAGAACCC CGGGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCTTGGTGAT TGAGTTCATC TACGCTATCG TGGGCATCGT CTGGCTCACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCTAAGAATG TCACCCTCGG AATGGTCGTC 
TGCAACTGGG TGGTCATTCT CAGCGTCTGC ATCACTGTCC TCTGCGTCTT TGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAATCT GCGGACTTAC 
AACCTGCGGC ACCGGTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG TCTAAAAGTG 
TTCCTCTGTT GCACCCGGAC AAAGGATTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCTG AATTTTTCCG TGACCTCGAC ATCGTGCCCT CCGACATCAT TGCTGGCCTG 
GTGCTGCTTA GACAGCGGCA GCGAGCCAAA CGCAATGCGG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT ACGCTACAAA GAAGTCTGCT ACTACATGCT CTTTGCCCTA 
GCCGCCTATG GCTGGCCCAT GTACCTGATG AGGAAGCCCA CCTGTGGTCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGCTG TCTCTGCCCT GCACGACCCC GATTCGCCCC TGGGGTCACC 
ATTGAGGAAG ACAACTGTTG TGGCTGCAAC GCAATCGCTA TCCGCCGTCA CTTCCTGGAT 
GAGAACATGA CTGCTGTAGA TATTGTCTAC ACCTCCTGTC ACGATGCGGT CTATGAAACT 
CCCTTCTACG TAGCTGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGAACA 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACAGGCGATG CTGAGCGCCT CCCTGTGGAG 
GGACACAGAG GCACCTGGTT GGGCCACAAG GGCATGGTAC TCTCAGCTGA GTACATCAAG 
AAGAAGTTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGTGGA 
ACCAAACACT ATGGCCTGAT TGTGGTAGGC CACTCCTTGG GAGCAGGCAC AGCCGCCATC 
CTCTCCTTCC TCCTGCGCCC CCAGTACCCG ACCCTCAAGT GCTTCGCTTA CTCCCCACCA 
GGAGGCCTGC TAAGTGAGGA TGCTATGGAA TACTCCAAAG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTCCC CAGGATTGGC CTTTCCCAGC TGGAAGGTTT CCGTCGGCAG 
CTTCTGGATG TCCTGCAGCG GAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGTATCC CCAAGTCGGA GCTGCCCGAG GATCAGGTGG AGGTGACCAC TCTGGCCAGC 
ACACGACTCT GGACACACCC CAGTGATTTG ACCATCGCCC TTTCGGCTAG CACCCCACTC 
TACCCACCTG GCCGCATCAT CCACGTGGTT CACAACCATC CTGCTGAGCA GTGCTGCTGC 
TGTGAGCAGG AGGAGCCCAC ATACTTTGCC ATCTGGGGCG ACAATAAAGC CTTTAACGAG 
GTGATCATCT CACCAGCCAT GCTGCATGAG CACCTGCCCT ATGTGGTCAT GGAGGGGCTC 
AATAAGGTGC TGGAGAATTA CAACAAAGGA AAGACAGCGC TGCTGTCTGC TGCAAAGGTC 
ATGGTGAGCC CCACCGAGGT AGACCTCACT CCCGAGCTCA TCTTCCAGCA ACAGCCGTTG 
CCCACGGGCC CACCTCTGCC CACTGGCCTG GCCCTGGAGC TGCCTGCCAC AGAGCATCGC 
AACAGCAGTG TCAGGAGCAA GTCTCAGTCT GAGATGAGTC TGGAGGGCTT CTCTGAGGGC 
CGGCTGCTGT CCCCAGTGGC TGCTGCATCA GCAGCCCGAC AAGACCCCGT AGAGCTTCTG 
CTGCTGTCCA CCCAGGAGCG ACTGGCAGCA GAGCTGCAGT CCCGGCGGGC ACCACTGGCC 
ACCATGGAGA GCCTCTCAGA CACTGAGTCA CTGTACAGCT TCGACTCACG CCGCTCCTCA 
GGCTTCCGAA GCATCAGAGG CTCGCCCAGC CTCCATGCAG TACTGGAGCG TGACGAGGGC 
CACCTGTTTT ACATCGACCC GGCCATTCCC GAGGAGAACC CGTCCCTCAG CTCACGCACA 
GAGCTGCTGG CAGCCGACAG TCTGTCCAAG CACTCACAGG ACACCCAACC CCTGGAGGCG 
GCTCTAGGCA GCGGGGGTGT GACTCCTGAG CGACCACCAA GTGCAACCAT TGAAGAGGAG 
GAGGCCGCAG GTGGCAGTGA GGGTGGTGGG GTGGCCCCCC GGGGAGAGCT GGCACTGCAC 
AATGGGCGCC TGGGGGACTC ACCCAGCCCT CAGGTGCTAG AATTCGCCGA GTTCATTGAC 
AGCCTCTTCA ACCTGGACAG CAAGAGCAGC TCCTTCCAGG ACCTCTACTG CATGATGGTG 
CCTGAGAGCC CCACTAGTGA CTACACTGAG GGCCCCAAGT CCCCCAGCCA ACAGGAAATC 
TTGCTTCGGG CTCAGTTTGA GCCCAACCTG GTACCCAAGC CCCCCAGGCT CTTTGCAGGA 
TCTGCTGAAC CCTCCTCTGG CATCTCGCTC TCACCCTCCT TCCCACTCAG CTCCTCGGGG 
GAGCTCATGG ACCTGACCCC CACGGGCCTC AGCAGCCAGG AGTGTCTGGC CACAGACAAG 
ATCCGGACTT CCACCCCCAC AGGCCACGGG GCCAGCCCCA CCAAGCAGGA TGACCTGGTC 
ATCTCGGCAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_932782.2
CDS	101..3235
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPTCGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEDQVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPLPTGLALELPATEHRNSSVRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQSRRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSATIEEEEAAGGSEGGGVAPRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMMVPESPTSDYTEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSAEPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLATDKIRTSTPTGHGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	NM_198114.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus diacylglycerol lipase, alpha (Dagla), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198114.2

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCTATCT TCCTCTTCCT GCTACACACC ACCTGGTTTG TAATCCTGTC TGTGGTGCTC 
TTCGGCTTGG TCTATAACCC ACACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGA 
GGCTACCTGG GCATCCTTCT GAGCTGTATG ATTGCCGAAA TGGCTATCAT CTGGCTGAGC 
ATGCGTGGGG GCATCCTCTA CACAGAACCC CGGGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCTTGGTGAT TGAGTTCATC TACGCTATCG TGGGCATCGT CTGGCTCACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCTAAGAATG TCACCCTCGG AATGGTCGTC 
TGCAACTGGG TGGTCATTCT CAGCGTCTGC ATCACTGTCC TCTGCGTCTT TGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAATCT GCGGACTTAC 
AACCTGCGGC ACCGGTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG TCTAAAAGTG 
TTCCTCTGTT GCACCCGGAC AAAGGATTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCTG AATTTTTCCG TGACCTCGAC ATCGTGCCCT CCGACATCAT TGCTGGCCTG 
GTGCTGCTTA GACAGCGGCA GCGAGCCAAA CGCAATGCGG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT ACGCTACAAA GAAGTCTGCT ACTACATGCT CTTTGCCCTA 
GCCGCCTATG GCTGGCCCAT GTACCTGATG AGGAAGCCCA CCTGTGGTCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGCTG TCTCTGCCCT GCACGACCCC GATTCGCCCC TGGGGTCACC 
ATTGAGGAAG ACAACTGTTG TGGCTGCAAC GCAATCGCTA TCCGCCGTCA CTTCCTGGAT 
GAGAACATGA CTGCTGTAGA TATTGTCTAC ACCTCCTGTC ACGATGCGGT CTATGAAACT 
CCCTTCTACG TAGCTGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGAACA 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACAGGCGATG CTGAGCGCCT CCCTGTGGAG 
GGACACAGAG GCACCTGGTT GGGCCACAAG GGCATGGTAC TCTCAGCTGA GTACATCAAG 
AAGAAGTTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGTGGA 
ACCAAACACT ATGGCCTGAT TGTGGTAGGC CACTCCTTGG GAGCAGGCAC AGCCGCCATC 
CTCTCCTTCC TCCTGCGCCC CCAGTACCCG ACCCTCAAGT GCTTCGCTTA CTCCCCACCA 
GGAGGCCTGC TAAGTGAGGA TGCTATGGAA TACTCCAAAG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTCCC CAGGATTGGC CTTTCCCAGC TGGAAGGTTT CCGTCGGCAG 
CTTCTGGATG TCCTGCAGCG GAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGTATCC CCAAGTCGGA GCTGCCCGAG GATCAGGTGG AGGTGACCAC TCTGGCCAGC 
ACACGACTCT GGACACACCC CAGTGATTTG ACCATCGCCC TTTCGGCTAG CACCCCACTC 
TACCCACCTG GCCGCATCAT CCACGTGGTT CACAACCATC CTGCTGAGCA GTGCTGCTGC 
TGTGAGCAGG AGGAGCCCAC ATACTTTGCC ATCTGGGGCG ACAATAAAGC CTTTAACGAG 
GTGATCATCT CACCAGCCAT GCTGCATGAG CACCTGCCCT ATGTGGTCAT GGAGGGGCTC 
AATAAGGTGC TGGAGAATTA CAACAAAGGA AAGACAGCGC TGCTGTCTGC TGCAAAGGTC 
ATGGTGAGCC CCACCGAGGT AGACCTCACT CCCGAGCTCA TCTTCCAGCA ACAGCCGTTG 
CCCACGGGCC CACCTCTGCC CACTGGCCTG GCCCTGGAGC TGCCTGCCAC AGAGCATCGC 
AACAGCAGTG TCAGGAGCAA GTCTCAGTCT GAGATGAGTC TGGAGGGCTT CTCTGAGGGC 
CGGCTGCTGT CCCCAGTGGC TGCTGCATCA GCAGCCCGAC AAGACCCCGT AGAGCTTCTG 
CTGCTGTCCA CCCAGGAGCG ACTGGCAGCA GAGCTGCAGT CCCGGCGGGC ACCACTGGCC 
ACCATGGAGA GCCTCTCAGA CACTGAGTCA CTGTACAGCT TCGACTCACG CCGCTCCTCA 
GGCTTCCGAA GCATCAGAGG CTCGCCCAGC CTCCATGCAG TACTGGAGCG TGACGAGGGC 
CACCTGTTTT ACATCGACCC GGCCATTCCC GAGGAGAACC CGTCCCTCAG CTCACGCACA 
GAGCTGCTGG CAGCCGACAG TCTGTCCAAG CACTCACAGG ACACCCAACC CCTGGAGGCG 
GCTCTAGGCA GCGGGGGTGT GACTCCTGAG CGACCACCAA GTGCAACCAT TGAAGAGGAG 
GAGGCCGCAG GTGGCAGTGA GGGTGGTGGG GTGGCCCCCC GGGGAGAGCT GGCACTGCAC 
AATGGGCGCC TGGGGGACTC ACCCAGCCCT CAGGTGCTAG AATTCGCCGA GTTCATTGAC 
AGCCTCTTCA ACCTGGACAG CAAGAGCAGC TCCTTCCAGG ACCTCTACTG CATGATGGTG 
CCTGAGAGCC CCACTAGTGA CTACACTGAG GGCCCCAAGT CCCCCAGCCA ACAGGAAATC 
TTGCTTCGGG CTCAGTTTGA GCCCAACCTG GTACCCAAGC CCCCCAGGCT CTTTGCAGGA 
TCTGCTGAAC CCTCCTCTGG CATCTCGCTC TCACCCTCCT TCCCACTCAG CTCCTCGGGG 
GAGCTCATGG ACCTGACCCC CACGGGCCTC AGCAGCCAGG AGTGTCTGGC CACAGACAAG 
ATCCGGACTT CCACCCCCAC AGGCCACGGG GCCAGCCCCA CCAAGCAGGA TGACCTGGTC 
ATCTCGGCAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_017318213.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_017173702.1 Mus musculus diacylglycerol lipase, alpha (Dagla), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu20995
Clone ID Related Accession (Same CDS sequence)	XM_017318213.1 , NM_198114.2 , XM_017318213.2 , XM_006527114.3 , XM_006527114.2
Accession Version	XM_017318213.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCTATCT TCCTCTTCCT GCTACACACC ACCTGGTTTG TAATCCTGTC TGTGGTGCTC 
TTCGGCTTGG TCTATAACCC ACACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGA 
GGCTACCTGG GCATCCTTCT GAGCTGTATG ATTGCCGAAA TGGCTATCAT CTGGCTGAGC 
ATGCGTGGGG GCATCCTCTA CACAGAACCC CGGGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCTTGGTGAT TGAGTTCATC TACGCTATCG TGGGCATCGT CTGGCTCACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCTAAGAATG TCACCCTCGG AATGGTCGTC 
TGCAACTGGG TGGTCATTCT CAGCGTCTGC ATCACTGTCC TCTGCGTCTT TGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAATCT GCGGACTTAC 
AACCTGCGGC ACCGGTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG TCTAAAAGTG 
TTCCTCTGTT GCACCCGGAC AAAGGATTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCTG AATTTTTCCG TGACCTCGAC ATCGTGCCCT CCGACATCAT TGCTGGCCTG 
GTGCTGCTTA GACAGCGGCA GCGAGCCAAA CGCAATGCGG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT ACGCTACAAA GAAGTCTGCT ACTACATGCT CTTTGCCCTA 
GCCGCCTATG GCTGGCCCAT GTACCTGATG AGGAAGCCCA CCTGTGGTCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGCTG TCTCTGCCCT GCACGACCCC GATTCGCCCC TGGGGTCACC 
ATTGAGGAAG ACAACTGTTG TGGCTGCAAC GCAATCGCTA TCCGCCGTCA CTTCCTGGAT 
GAGAACATGA CTGCTGTAGA TATTGTCTAC ACCTCCTGTC ACGATGCGGT CTATGAAACT 
CCCTTCTACG TAGCTGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGAACA 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACAGGCGATG CTGAGCGCCT CCCTGTGGAG 
GGACACAGAG GCACCTGGTT GGGCCACAAG GGCATGGTAC TCTCAGCTGA GTACATCAAG 
AAGAAGTTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGTGGA 
ACCAAACACT ATGGCCTGAT TGTGGTAGGC CACTCCTTGG GAGCAGGCAC AGCCGCCATC 
CTCTCCTTCC TCCTGCGCCC CCAGTACCCG ACCCTCAAGT GCTTCGCTTA CTCCCCACCA 
GGAGGCCTGC TAAGTGAGGA TGCTATGGAA TACTCCAAAG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTCCC CAGGATTGGC CTTTCCCAGC TGGAAGGTTT CCGTCGGCAG 
CTTCTGGATG TCCTGCAGCG GAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGTATCC CCAAGTCGGA GCTGCCCGAG GATCAGGTGG AGGTGACCAC TCTGGCCAGC 
ACACGACTCT GGACACACCC CAGTGATTTG ACCATCGCCC TTTCGGCTAG CACCCCACTC 
TACCCACCTG GCCGCATCAT CCACGTGGTT CACAACCATC CTGCTGAGCA GTGCTGCTGC 
TGTGAGCAGG AGGAGCCCAC ATACTTTGCC ATCTGGGGCG ACAATAAAGC CTTTAACGAG 
GTGATCATCT CACCAGCCAT GCTGCATGAG CACCTGCCCT ATGTGGTCAT GGAGGGGCTC 
AATAAGGTGC TGGAGAATTA CAACAAAGGA AAGACAGCGC TGCTGTCTGC TGCAAAGGTC 
ATGGTGAGCC CCACCGAGGT AGACCTCACT CCCGAGCTCA TCTTCCAGCA ACAGCCGTTG 
CCCACGGGCC CACCTCTGCC CACTGGCCTG GCCCTGGAGC TGCCTGCCAC AGAGCATCGC 
AACAGCAGTG TCAGGAGCAA GTCTCAGTCT GAGATGAGTC TGGAGGGCTT CTCTGAGGGC 
CGGCTGCTGT CCCCAGTGGC TGCTGCATCA GCAGCCCGAC AAGACCCCGT AGAGCTTCTG 
CTGCTGTCCA CCCAGGAGCG ACTGGCAGCA GAGCTGCAGT CCCGGCGGGC ACCACTGGCC 
ACCATGGAGA GCCTCTCAGA CACTGAGTCA CTGTACAGCT TCGACTCACG CCGCTCCTCA 
GGCTTCCGAA GCATCAGAGG CTCGCCCAGC CTCCATGCAG TACTGGAGCG TGACGAGGGC 
CACCTGTTTT ACATCGACCC GGCCATTCCC GAGGAGAACC CGTCCCTCAG CTCACGCACA 
GAGCTGCTGG CAGCCGACAG TCTGTCCAAG CACTCACAGG ACACCCAACC CCTGGAGGCG 
GCTCTAGGCA GCGGGGGTGT GACTCCTGAG CGACCACCAA GTGCAACCAT TGAAGAGGAG 
GAGGCCGCAG GTGGCAGTGA GGGTGGTGGG GTGGCCCCCC GGGGAGAGCT GGCACTGCAC 
AATGGGCGCC TGGGGGACTC ACCCAGCCCT CAGGTGCTAG AATTCGCCGA GTTCATTGAC 
AGCCTCTTCA ACCTGGACAG CAAGAGCAGC TCCTTCCAGG ACCTCTACTG CATGATGGTG 
CCTGAGAGCC CCACTAGTGA CTACACTGAG GGCCCCAAGT CCCCCAGCCA ACAGGAAATC 
TTGCTTCGGG CTCAGTTTGA GCCCAACCTG GTACCCAAGC CCCCCAGGCT CTTTGCAGGA 
TCTGCTGAAC CCTCCTCTGG CATCTCGCTC TCACCCTCCT TCCCACTCAG CTCCTCGGGG 
GAGCTCATGG ACCTGACCCC CACGGGCCTC AGCAGCCAGG AGTGTCTGGC CACAGACAAG 
ATCCGGACTT CCACCCCCAC AGGCCACGGG GCCAGCCCCA CCAAGCAGGA TGACCTGGTC 
ATCTCGGCAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173702.1
CDS	62..3196
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPTCGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEDQVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPLPTGLALELPATEHRNSSVRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQSRRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSATIEEEEAAGGSEGGGVAPRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMMVPESPTSDYTEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSAEPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLATDKIRTSTPTGHGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	XM_017318213.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus diacylglycerol lipase, alpha (Dagla), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318213.1

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCTATCT TCCTCTTCCT GCTACACACC ACCTGGTTTG TAATCCTGTC TGTGGTGCTC 
TTCGGCTTGG TCTATAACCC ACACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGA 
GGCTACCTGG GCATCCTTCT GAGCTGTATG ATTGCCGAAA TGGCTATCAT CTGGCTGAGC 
ATGCGTGGGG GCATCCTCTA CACAGAACCC CGGGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCTTGGTGAT TGAGTTCATC TACGCTATCG TGGGCATCGT CTGGCTCACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCTAAGAATG TCACCCTCGG AATGGTCGTC 
TGCAACTGGG TGGTCATTCT CAGCGTCTGC ATCACTGTCC TCTGCGTCTT TGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAATCT GCGGACTTAC 
AACCTGCGGC ACCGGTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG TCTAAAAGTG 
TTCCTCTGTT GCACCCGGAC AAAGGATTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCTG AATTTTTCCG TGACCTCGAC ATCGTGCCCT CCGACATCAT TGCTGGCCTG 
GTGCTGCTTA GACAGCGGCA GCGAGCCAAA CGCAATGCGG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT ACGCTACAAA GAAGTCTGCT ACTACATGCT CTTTGCCCTA 
GCCGCCTATG GCTGGCCCAT GTACCTGATG AGGAAGCCCA CCTGTGGTCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGCTG TCTCTGCCCT GCACGACCCC GATTCGCCCC TGGGGTCACC 
ATTGAGGAAG ACAACTGTTG TGGCTGCAAC GCAATCGCTA TCCGCCGTCA CTTCCTGGAT 
GAGAACATGA CTGCTGTAGA TATTGTCTAC ACCTCCTGTC ACGATGCGGT CTATGAAACT 
CCCTTCTACG TAGCTGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGAACA 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACAGGCGATG CTGAGCGCCT CCCTGTGGAG 
GGACACAGAG GCACCTGGTT GGGCCACAAG GGCATGGTAC TCTCAGCTGA GTACATCAAG 
AAGAAGTTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGTGGA 
ACCAAACACT ATGGCCTGAT TGTGGTAGGC CACTCCTTGG GAGCAGGCAC AGCCGCCATC 
CTCTCCTTCC TCCTGCGCCC CCAGTACCCG ACCCTCAAGT GCTTCGCTTA CTCCCCACCA 
GGAGGCCTGC TAAGTGAGGA TGCTATGGAA TACTCCAAAG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTCCC CAGGATTGGC CTTTCCCAGC TGGAAGGTTT CCGTCGGCAG 
CTTCTGGATG TCCTGCAGCG GAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGTATCC CCAAGTCGGA GCTGCCCGAG GATCAGGTGG AGGTGACCAC TCTGGCCAGC 
ACACGACTCT GGACACACCC CAGTGATTTG ACCATCGCCC TTTCGGCTAG CACCCCACTC 
TACCCACCTG GCCGCATCAT CCACGTGGTT CACAACCATC CTGCTGAGCA GTGCTGCTGC 
TGTGAGCAGG AGGAGCCCAC ATACTTTGCC ATCTGGGGCG ACAATAAAGC CTTTAACGAG 
GTGATCATCT CACCAGCCAT GCTGCATGAG CACCTGCCCT ATGTGGTCAT GGAGGGGCTC 
AATAAGGTGC TGGAGAATTA CAACAAAGGA AAGACAGCGC TGCTGTCTGC TGCAAAGGTC 
ATGGTGAGCC CCACCGAGGT AGACCTCACT CCCGAGCTCA TCTTCCAGCA ACAGCCGTTG 
CCCACGGGCC CACCTCTGCC CACTGGCCTG GCCCTGGAGC TGCCTGCCAC AGAGCATCGC 
AACAGCAGTG TCAGGAGCAA GTCTCAGTCT GAGATGAGTC TGGAGGGCTT CTCTGAGGGC 
CGGCTGCTGT CCCCAGTGGC TGCTGCATCA GCAGCCCGAC AAGACCCCGT AGAGCTTCTG 
CTGCTGTCCA CCCAGGAGCG ACTGGCAGCA GAGCTGCAGT CCCGGCGGGC ACCACTGGCC 
ACCATGGAGA GCCTCTCAGA CACTGAGTCA CTGTACAGCT TCGACTCACG CCGCTCCTCA 
GGCTTCCGAA GCATCAGAGG CTCGCCCAGC CTCCATGCAG TACTGGAGCG TGACGAGGGC 
CACCTGTTTT ACATCGACCC GGCCATTCCC GAGGAGAACC CGTCCCTCAG CTCACGCACA 
GAGCTGCTGG CAGCCGACAG TCTGTCCAAG CACTCACAGG ACACCCAACC CCTGGAGGCG 
GCTCTAGGCA GCGGGGGTGT GACTCCTGAG CGACCACCAA GTGCAACCAT TGAAGAGGAG 
GAGGCCGCAG GTGGCAGTGA GGGTGGTGGG GTGGCCCCCC GGGGAGAGCT GGCACTGCAC 
AATGGGCGCC TGGGGGACTC ACCCAGCCCT CAGGTGCTAG AATTCGCCGA GTTCATTGAC 
AGCCTCTTCA ACCTGGACAG CAAGAGCAGC TCCTTCCAGG ACCTCTACTG CATGATGGTG 
CCTGAGAGCC CCACTAGTGA CTACACTGAG GGCCCCAAGT CCCCCAGCCA ACAGGAAATC 
TTGCTTCGGG CTCAGTTTGA GCCCAACCTG GTACCCAAGC CCCCCAGGCT CTTTGCAGGA 
TCTGCTGAAC CCTCCTCTGG CATCTCGCTC TCACCCTCCT TCCCACTCAG CTCCTCGGGG 
GAGCTCATGG ACCTGACCCC CACGGGCCTC AGCAGCCAGG AGTGTCTGGC CACAGACAAG 
ATCCGGACTT CCACCCCCAC AGGCCACGGG GCCAGCCCCA CCAAGCAGGA TGACCTGGTC 
ATCTCGGCAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_017318213.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_017173702.1 Mus musculus diacylglycerol lipase, alpha (Dagla), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu20995
Clone ID Related Accession (Same CDS sequence)	XM_017318213.1 , NM_198114.2 , XM_017318213.2 , XM_006527114.3 , XM_006527114.2
Accession Version	XM_017318213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318213.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCTATCT TCCTCTTCCT GCTACACACC ACCTGGTTTG TAATCCTGTC TGTGGTGCTC 
TTCGGCTTGG TCTATAACCC ACACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGA 
GGCTACCTGG GCATCCTTCT GAGCTGTATG ATTGCCGAAA TGGCTATCAT CTGGCTGAGC 
ATGCGTGGGG GCATCCTCTA CACAGAACCC CGGGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCTTGGTGAT TGAGTTCATC TACGCTATCG TGGGCATCGT CTGGCTCACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCTAAGAATG TCACCCTCGG AATGGTCGTC 
TGCAACTGGG TGGTCATTCT CAGCGTCTGC ATCACTGTCC TCTGCGTCTT TGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAATCT GCGGACTTAC 
AACCTGCGGC ACCGGTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG TCTAAAAGTG 
TTCCTCTGTT GCACCCGGAC AAAGGATTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCTG AATTTTTCCG TGACCTCGAC ATCGTGCCCT CCGACATCAT TGCTGGCCTG 
GTGCTGCTTA GACAGCGGCA GCGAGCCAAA CGCAATGCGG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT ACGCTACAAA GAAGTCTGCT ACTACATGCT CTTTGCCCTA 
GCCGCCTATG GCTGGCCCAT GTACCTGATG AGGAAGCCCA CCTGTGGTCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGCTG TCTCTGCCCT GCACGACCCC GATTCGCCCC TGGGGTCACC 
ATTGAGGAAG ACAACTGTTG TGGCTGCAAC GCAATCGCTA TCCGCCGTCA CTTCCTGGAT 
GAGAACATGA CTGCTGTAGA TATTGTCTAC ACCTCCTGTC ACGATGCGGT CTATGAAACT 
CCCTTCTACG TAGCTGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGAACA 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACAGGCGATG CTGAGCGCCT CCCTGTGGAG 
GGACACAGAG GCACCTGGTT GGGCCACAAG GGCATGGTAC TCTCAGCTGA GTACATCAAG 
AAGAAGTTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGTGGA 
ACCAAACACT ATGGCCTGAT TGTGGTAGGC CACTCCTTGG GAGCAGGCAC AGCCGCCATC 
CTCTCCTTCC TCCTGCGCCC CCAGTACCCG ACCCTCAAGT GCTTCGCTTA CTCCCCACCA 
GGAGGCCTGC TAAGTGAGGA TGCTATGGAA TACTCCAAAG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTCCC CAGGATTGGC CTTTCCCAGC TGGAAGGTTT CCGTCGGCAG 
CTTCTGGATG TCCTGCAGCG GAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGTATCC CCAAGTCGGA GCTGCCCGAG GATCAGGTGG AGGTGACCAC TCTGGCCAGC 
ACACGACTCT GGACACACCC CAGTGATTTG ACCATCGCCC TTTCGGCTAG CACCCCACTC 
TACCCACCTG GCCGCATCAT CCACGTGGTT CACAACCATC CTGCTGAGCA GTGCTGCTGC 
TGTGAGCAGG AGGAGCCCAC ATACTTTGCC ATCTGGGGCG ACAATAAAGC CTTTAACGAG 
GTGATCATCT CACCAGCCAT GCTGCATGAG CACCTGCCCT ATGTGGTCAT GGAGGGGCTC 
AATAAGGTGC TGGAGAATTA CAACAAAGGA AAGACAGCGC TGCTGTCTGC TGCAAAGGTC 
ATGGTGAGCC CCACCGAGGT AGACCTCACT CCCGAGCTCA TCTTCCAGCA ACAGCCGTTG 
CCCACGGGCC CACCTCTGCC CACTGGCCTG GCCCTGGAGC TGCCTGCCAC AGAGCATCGC 
AACAGCAGTG TCAGGAGCAA GTCTCAGTCT GAGATGAGTC TGGAGGGCTT CTCTGAGGGC 
CGGCTGCTGT CCCCAGTGGC TGCTGCATCA GCAGCCCGAC AAGACCCCGT AGAGCTTCTG 
CTGCTGTCCA CCCAGGAGCG ACTGGCAGCA GAGCTGCAGT CCCGGCGGGC ACCACTGGCC 
ACCATGGAGA GCCTCTCAGA CACTGAGTCA CTGTACAGCT TCGACTCACG CCGCTCCTCA 
GGCTTCCGAA GCATCAGAGG CTCGCCCAGC CTCCATGCAG TACTGGAGCG TGACGAGGGC 
CACCTGTTTT ACATCGACCC GGCCATTCCC GAGGAGAACC CGTCCCTCAG CTCACGCACA 
GAGCTGCTGG CAGCCGACAG TCTGTCCAAG CACTCACAGG ACACCCAACC CCTGGAGGCG 
GCTCTAGGCA GCGGGGGTGT GACTCCTGAG CGACCACCAA GTGCAACCAT TGAAGAGGAG 
GAGGCCGCAG GTGGCAGTGA GGGTGGTGGG GTGGCCCCCC GGGGAGAGCT GGCACTGCAC 
AATGGGCGCC TGGGGGACTC ACCCAGCCCT CAGGTGCTAG AATTCGCCGA GTTCATTGAC 
AGCCTCTTCA ACCTGGACAG CAAGAGCAGC TCCTTCCAGG ACCTCTACTG CATGATGGTG 
CCTGAGAGCC CCACTAGTGA CTACACTGAG GGCCCCAAGT CCCCCAGCCA ACAGGAAATC 
TTGCTTCGGG CTCAGTTTGA GCCCAACCTG GTACCCAAGC CCCCCAGGCT CTTTGCAGGA 
TCTGCTGAAC CCTCCTCTGG CATCTCGCTC TCACCCTCCT TCCCACTCAG CTCCTCGGGG 
GAGCTCATGG ACCTGACCCC CACGGGCCTC AGCAGCCAGG AGTGTCTGGC CACAGACAAG 
ATCCGGACTT CCACCCCCAC AGGCCACGGG GCCAGCCCCA CCAAGCAGGA TGACCTGGTC 
ATCTCGGCAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173702.1
CDS	110..3244
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPTCGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEDQVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPLPTGLALELPATEHRNSSVRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQSRRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSATIEEEEAAGGSEGGGVAPRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMMVPESPTSDYTEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSAEPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLATDKIRTSTPTGHGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	XM_017318213.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus diacylglycerol lipase, alpha (Dagla), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318213.2

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCTATCT TCCTCTTCCT GCTACACACC ACCTGGTTTG TAATCCTGTC TGTGGTGCTC 
TTCGGCTTGG TCTATAACCC ACACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGA 
GGCTACCTGG GCATCCTTCT GAGCTGTATG ATTGCCGAAA TGGCTATCAT CTGGCTGAGC 
ATGCGTGGGG GCATCCTCTA CACAGAACCC CGGGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCTTGGTGAT TGAGTTCATC TACGCTATCG TGGGCATCGT CTGGCTCACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCTAAGAATG TCACCCTCGG AATGGTCGTC 
TGCAACTGGG TGGTCATTCT CAGCGTCTGC ATCACTGTCC TCTGCGTCTT TGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAATCT GCGGACTTAC 
AACCTGCGGC ACCGGTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG TCTAAAAGTG 
TTCCTCTGTT GCACCCGGAC AAAGGATTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCTG AATTTTTCCG TGACCTCGAC ATCGTGCCCT CCGACATCAT TGCTGGCCTG 
GTGCTGCTTA GACAGCGGCA GCGAGCCAAA CGCAATGCGG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT ACGCTACAAA GAAGTCTGCT ACTACATGCT CTTTGCCCTA 
GCCGCCTATG GCTGGCCCAT GTACCTGATG AGGAAGCCCA CCTGTGGTCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGCTG TCTCTGCCCT GCACGACCCC GATTCGCCCC TGGGGTCACC 
ATTGAGGAAG ACAACTGTTG TGGCTGCAAC GCAATCGCTA TCCGCCGTCA CTTCCTGGAT 
GAGAACATGA CTGCTGTAGA TATTGTCTAC ACCTCCTGTC ACGATGCGGT CTATGAAACT 
CCCTTCTACG TAGCTGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGAACA 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACAGGCGATG CTGAGCGCCT CCCTGTGGAG 
GGACACAGAG GCACCTGGTT GGGCCACAAG GGCATGGTAC TCTCAGCTGA GTACATCAAG 
AAGAAGTTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGTGGA 
ACCAAACACT ATGGCCTGAT TGTGGTAGGC CACTCCTTGG GAGCAGGCAC AGCCGCCATC 
CTCTCCTTCC TCCTGCGCCC CCAGTACCCG ACCCTCAAGT GCTTCGCTTA CTCCCCACCA 
GGAGGCCTGC TAAGTGAGGA TGCTATGGAA TACTCCAAAG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTCCC CAGGATTGGC CTTTCCCAGC TGGAAGGTTT CCGTCGGCAG 
CTTCTGGATG TCCTGCAGCG GAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGTATCC CCAAGTCGGA GCTGCCCGAG GATCAGGTGG AGGTGACCAC TCTGGCCAGC 
ACACGACTCT GGACACACCC CAGTGATTTG ACCATCGCCC TTTCGGCTAG CACCCCACTC 
TACCCACCTG GCCGCATCAT CCACGTGGTT CACAACCATC CTGCTGAGCA GTGCTGCTGC 
TGTGAGCAGG AGGAGCCCAC ATACTTTGCC ATCTGGGGCG ACAATAAAGC CTTTAACGAG 
GTGATCATCT CACCAGCCAT GCTGCATGAG CACCTGCCCT ATGTGGTCAT GGAGGGGCTC 
AATAAGGTGC TGGAGAATTA CAACAAAGGA AAGACAGCGC TGCTGTCTGC TGCAAAGGTC 
ATGGTGAGCC CCACCGAGGT AGACCTCACT CCCGAGCTCA TCTTCCAGCA ACAGCCGTTG 
CCCACGGGCC CACCTCTGCC CACTGGCCTG GCCCTGGAGC TGCCTGCCAC AGAGCATCGC 
AACAGCAGTG TCAGGAGCAA GTCTCAGTCT GAGATGAGTC TGGAGGGCTT CTCTGAGGGC 
CGGCTGCTGT CCCCAGTGGC TGCTGCATCA GCAGCCCGAC AAGACCCCGT AGAGCTTCTG 
CTGCTGTCCA CCCAGGAGCG ACTGGCAGCA GAGCTGCAGT CCCGGCGGGC ACCACTGGCC 
ACCATGGAGA GCCTCTCAGA CACTGAGTCA CTGTACAGCT TCGACTCACG CCGCTCCTCA 
GGCTTCCGAA GCATCAGAGG CTCGCCCAGC CTCCATGCAG TACTGGAGCG TGACGAGGGC 
CACCTGTTTT ACATCGACCC GGCCATTCCC GAGGAGAACC CGTCCCTCAG CTCACGCACA 
GAGCTGCTGG CAGCCGACAG TCTGTCCAAG CACTCACAGG ACACCCAACC CCTGGAGGCG 
GCTCTAGGCA GCGGGGGTGT GACTCCTGAG CGACCACCAA GTGCAACCAT TGAAGAGGAG 
GAGGCCGCAG GTGGCAGTGA GGGTGGTGGG GTGGCCCCCC GGGGAGAGCT GGCACTGCAC 
AATGGGCGCC TGGGGGACTC ACCCAGCCCT CAGGTGCTAG AATTCGCCGA GTTCATTGAC 
AGCCTCTTCA ACCTGGACAG CAAGAGCAGC TCCTTCCAGG ACCTCTACTG CATGATGGTG 
CCTGAGAGCC CCACTAGTGA CTACACTGAG GGCCCCAAGT CCCCCAGCCA ACAGGAAATC 
TTGCTTCGGG CTCAGTTTGA GCCCAACCTG GTACCCAAGC CCCCCAGGCT CTTTGCAGGA 
TCTGCTGAAC CCTCCTCTGG CATCTCGCTC TCACCCTCCT TCCCACTCAG CTCCTCGGGG 
GAGCTCATGG ACCTGACCCC CACGGGCCTC AGCAGCCAGG AGTGTCTGGC CACAGACAAG 
ATCCGGACTT CCACCCCCAC AGGCCACGGG GCCAGCCCCA CCAAGCAGGA TGACCTGGTC 
ATCTCGGCAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_006527114.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006527177.1 Mus musculus diacylglycerol lipase, alpha (Dagla), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20995
Clone ID Related Accession (Same CDS sequence)	XM_017318213.1 , NM_198114.2 , XM_017318213.2 , XM_006527114.3 , XM_006527114.2
Accession Version	XM_006527114.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527114.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCTATCT TCCTCTTCCT GCTACACACC ACCTGGTTTG TAATCCTGTC TGTGGTGCTC 
TTCGGCTTGG TCTATAACCC ACACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGA 
GGCTACCTGG GCATCCTTCT GAGCTGTATG ATTGCCGAAA TGGCTATCAT CTGGCTGAGC 
ATGCGTGGGG GCATCCTCTA CACAGAACCC CGGGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCTTGGTGAT TGAGTTCATC TACGCTATCG TGGGCATCGT CTGGCTCACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCTAAGAATG TCACCCTCGG AATGGTCGTC 
TGCAACTGGG TGGTCATTCT CAGCGTCTGC ATCACTGTCC TCTGCGTCTT TGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAATCT GCGGACTTAC 
AACCTGCGGC ACCGGTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG TCTAAAAGTG 
TTCCTCTGTT GCACCCGGAC AAAGGATTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCTG AATTTTTCCG TGACCTCGAC ATCGTGCCCT CCGACATCAT TGCTGGCCTG 
GTGCTGCTTA GACAGCGGCA GCGAGCCAAA CGCAATGCGG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT ACGCTACAAA GAAGTCTGCT ACTACATGCT CTTTGCCCTA 
GCCGCCTATG GCTGGCCCAT GTACCTGATG AGGAAGCCCA CCTGTGGTCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGCTG TCTCTGCCCT GCACGACCCC GATTCGCCCC TGGGGTCACC 
ATTGAGGAAG ACAACTGTTG TGGCTGCAAC GCAATCGCTA TCCGCCGTCA CTTCCTGGAT 
GAGAACATGA CTGCTGTAGA TATTGTCTAC ACCTCCTGTC ACGATGCGGT CTATGAAACT 
CCCTTCTACG TAGCTGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGAACA 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACAGGCGATG CTGAGCGCCT CCCTGTGGAG 
GGACACAGAG GCACCTGGTT GGGCCACAAG GGCATGGTAC TCTCAGCTGA GTACATCAAG 
AAGAAGTTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGTGGA 
ACCAAACACT ATGGCCTGAT TGTGGTAGGC CACTCCTTGG GAGCAGGCAC AGCCGCCATC 
CTCTCCTTCC TCCTGCGCCC CCAGTACCCG ACCCTCAAGT GCTTCGCTTA CTCCCCACCA 
GGAGGCCTGC TAAGTGAGGA TGCTATGGAA TACTCCAAAG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTCCC CAGGATTGGC CTTTCCCAGC TGGAAGGTTT CCGTCGGCAG 
CTTCTGGATG TCCTGCAGCG GAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGTATCC CCAAGTCGGA GCTGCCCGAG GATCAGGTGG AGGTGACCAC TCTGGCCAGC 
ACACGACTCT GGACACACCC CAGTGATTTG ACCATCGCCC TTTCGGCTAG CACCCCACTC 
TACCCACCTG GCCGCATCAT CCACGTGGTT CACAACCATC CTGCTGAGCA GTGCTGCTGC 
TGTGAGCAGG AGGAGCCCAC ATACTTTGCC ATCTGGGGCG ACAATAAAGC CTTTAACGAG 
GTGATCATCT CACCAGCCAT GCTGCATGAG CACCTGCCCT ATGTGGTCAT GGAGGGGCTC 
AATAAGGTGC TGGAGAATTA CAACAAAGGA AAGACAGCGC TGCTGTCTGC TGCAAAGGTC 
ATGGTGAGCC CCACCGAGGT AGACCTCACT CCCGAGCTCA TCTTCCAGCA ACAGCCGTTG 
CCCACGGGCC CACCTCTGCC CACTGGCCTG GCCCTGGAGC TGCCTGCCAC AGAGCATCGC 
AACAGCAGTG TCAGGAGCAA GTCTCAGTCT GAGATGAGTC TGGAGGGCTT CTCTGAGGGC 
CGGCTGCTGT CCCCAGTGGC TGCTGCATCA GCAGCCCGAC AAGACCCCGT AGAGCTTCTG 
CTGCTGTCCA CCCAGGAGCG ACTGGCAGCA GAGCTGCAGT CCCGGCGGGC ACCACTGGCC 
ACCATGGAGA GCCTCTCAGA CACTGAGTCA CTGTACAGCT TCGACTCACG CCGCTCCTCA 
GGCTTCCGAA GCATCAGAGG CTCGCCCAGC CTCCATGCAG TACTGGAGCG TGACGAGGGC 
CACCTGTTTT ACATCGACCC GGCCATTCCC GAGGAGAACC CGTCCCTCAG CTCACGCACA 
GAGCTGCTGG CAGCCGACAG TCTGTCCAAG CACTCACAGG ACACCCAACC CCTGGAGGCG 
GCTCTAGGCA GCGGGGGTGT GACTCCTGAG CGACCACCAA GTGCAACCAT TGAAGAGGAG 
GAGGCCGCAG GTGGCAGTGA GGGTGGTGGG GTGGCCCCCC GGGGAGAGCT GGCACTGCAC 
AATGGGCGCC TGGGGGACTC ACCCAGCCCT CAGGTGCTAG AATTCGCCGA GTTCATTGAC 
AGCCTCTTCA ACCTGGACAG CAAGAGCAGC TCCTTCCAGG ACCTCTACTG CATGATGGTG 
CCTGAGAGCC CCACTAGTGA CTACACTGAG GGCCCCAAGT CCCCCAGCCA ACAGGAAATC 
TTGCTTCGGG CTCAGTTTGA GCCCAACCTG GTACCCAAGC CCCCCAGGCT CTTTGCAGGA 
TCTGCTGAAC CCTCCTCTGG CATCTCGCTC TCACCCTCCT TCCCACTCAG CTCCTCGGGG 
GAGCTCATGG ACCTGACCCC CACGGGCCTC AGCAGCCAGG AGTGTCTGGC CACAGACAAG 
ATCCGGACTT CCACCCCCAC AGGCCACGGG GCCAGCCCCA CCAAGCAGGA TGACCTGGTC 
ATCTCGGCAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527177.1
CDS	573..3707
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPTCGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEDQVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPLPTGLALELPATEHRNSSVRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQSRRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSATIEEEEAAGGSEGGGVAPRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMMVPESPTSDYTEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSAEPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLATDKIRTSTPTGHGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	XM_006527114.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus diacylglycerol lipase, alpha (Dagla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527114.3

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCTATCT TCCTCTTCCT GCTACACACC ACCTGGTTTG TAATCCTGTC TGTGGTGCTC 
TTCGGCTTGG TCTATAACCC ACACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGA 
GGCTACCTGG GCATCCTTCT GAGCTGTATG ATTGCCGAAA TGGCTATCAT CTGGCTGAGC 
ATGCGTGGGG GCATCCTCTA CACAGAACCC CGGGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCTTGGTGAT TGAGTTCATC TACGCTATCG TGGGCATCGT CTGGCTCACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCTAAGAATG TCACCCTCGG AATGGTCGTC 
TGCAACTGGG TGGTCATTCT CAGCGTCTGC ATCACTGTCC TCTGCGTCTT TGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAATCT GCGGACTTAC 
AACCTGCGGC ACCGGTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG TCTAAAAGTG 
TTCCTCTGTT GCACCCGGAC AAAGGATTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCTG AATTTTTCCG TGACCTCGAC ATCGTGCCCT CCGACATCAT TGCTGGCCTG 
GTGCTGCTTA GACAGCGGCA GCGAGCCAAA CGCAATGCGG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT ACGCTACAAA GAAGTCTGCT ACTACATGCT CTTTGCCCTA 
GCCGCCTATG GCTGGCCCAT GTACCTGATG AGGAAGCCCA CCTGTGGTCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGCTG TCTCTGCCCT GCACGACCCC GATTCGCCCC TGGGGTCACC 
ATTGAGGAAG ACAACTGTTG TGGCTGCAAC GCAATCGCTA TCCGCCGTCA CTTCCTGGAT 
GAGAACATGA CTGCTGTAGA TATTGTCTAC ACCTCCTGTC ACGATGCGGT CTATGAAACT 
CCCTTCTACG TAGCTGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGAACA 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACAGGCGATG CTGAGCGCCT CCCTGTGGAG 
GGACACAGAG GCACCTGGTT GGGCCACAAG GGCATGGTAC TCTCAGCTGA GTACATCAAG 
AAGAAGTTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGTGGA 
ACCAAACACT ATGGCCTGAT TGTGGTAGGC CACTCCTTGG GAGCAGGCAC AGCCGCCATC 
CTCTCCTTCC TCCTGCGCCC CCAGTACCCG ACCCTCAAGT GCTTCGCTTA CTCCCCACCA 
GGAGGCCTGC TAAGTGAGGA TGCTATGGAA TACTCCAAAG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTCCC CAGGATTGGC CTTTCCCAGC TGGAAGGTTT CCGTCGGCAG 
CTTCTGGATG TCCTGCAGCG GAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGTATCC CCAAGTCGGA GCTGCCCGAG GATCAGGTGG AGGTGACCAC TCTGGCCAGC 
ACACGACTCT GGACACACCC CAGTGATTTG ACCATCGCCC TTTCGGCTAG CACCCCACTC 
TACCCACCTG GCCGCATCAT CCACGTGGTT CACAACCATC CTGCTGAGCA GTGCTGCTGC 
TGTGAGCAGG AGGAGCCCAC ATACTTTGCC ATCTGGGGCG ACAATAAAGC CTTTAACGAG 
GTGATCATCT CACCAGCCAT GCTGCATGAG CACCTGCCCT ATGTGGTCAT GGAGGGGCTC 
AATAAGGTGC TGGAGAATTA CAACAAAGGA AAGACAGCGC TGCTGTCTGC TGCAAAGGTC 
ATGGTGAGCC CCACCGAGGT AGACCTCACT CCCGAGCTCA TCTTCCAGCA ACAGCCGTTG 
CCCACGGGCC CACCTCTGCC CACTGGCCTG GCCCTGGAGC TGCCTGCCAC AGAGCATCGC 
AACAGCAGTG TCAGGAGCAA GTCTCAGTCT GAGATGAGTC TGGAGGGCTT CTCTGAGGGC 
CGGCTGCTGT CCCCAGTGGC TGCTGCATCA GCAGCCCGAC AAGACCCCGT AGAGCTTCTG 
CTGCTGTCCA CCCAGGAGCG ACTGGCAGCA GAGCTGCAGT CCCGGCGGGC ACCACTGGCC 
ACCATGGAGA GCCTCTCAGA CACTGAGTCA CTGTACAGCT TCGACTCACG CCGCTCCTCA 
GGCTTCCGAA GCATCAGAGG CTCGCCCAGC CTCCATGCAG TACTGGAGCG TGACGAGGGC 
CACCTGTTTT ACATCGACCC GGCCATTCCC GAGGAGAACC CGTCCCTCAG CTCACGCACA 
GAGCTGCTGG CAGCCGACAG TCTGTCCAAG CACTCACAGG ACACCCAACC CCTGGAGGCG 
GCTCTAGGCA GCGGGGGTGT GACTCCTGAG CGACCACCAA GTGCAACCAT TGAAGAGGAG 
GAGGCCGCAG GTGGCAGTGA GGGTGGTGGG GTGGCCCCCC GGGGAGAGCT GGCACTGCAC 
AATGGGCGCC TGGGGGACTC ACCCAGCCCT CAGGTGCTAG AATTCGCCGA GTTCATTGAC 
AGCCTCTTCA ACCTGGACAG CAAGAGCAGC TCCTTCCAGG ACCTCTACTG CATGATGGTG 
CCTGAGAGCC CCACTAGTGA CTACACTGAG GGCCCCAAGT CCCCCAGCCA ACAGGAAATC 
TTGCTTCGGG CTCAGTTTGA GCCCAACCTG GTACCCAAGC CCCCCAGGCT CTTTGCAGGA 
TCTGCTGAAC CCTCCTCTGG CATCTCGCTC TCACCCTCCT TCCCACTCAG CTCCTCGGGG 
GAGCTCATGG ACCTGACCCC CACGGGCCTC AGCAGCCAGG AGTGTCTGGC CACAGACAAG 
ATCCGGACTT CCACCCCCAC AGGCCACGGG GCCAGCCCCA CCAAGCAGGA TGACCTGGTC 
ATCTCGGCAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_006527114.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006527177.1 Mus musculus diacylglycerol lipase, alpha (Dagla), transcript variant X1, mRNA.

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CloneID	OMu20995
Clone ID Related Accession (Same CDS sequence)	XM_017318213.1 , NM_198114.2 , XM_017318213.2 , XM_006527114.3 , XM_006527114.2
Accession Version	XM_006527114.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCTATCT TCCTCTTCCT GCTACACACC ACCTGGTTTG TAATCCTGTC TGTGGTGCTC 
TTCGGCTTGG TCTATAACCC ACACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGA 
GGCTACCTGG GCATCCTTCT GAGCTGTATG ATTGCCGAAA TGGCTATCAT CTGGCTGAGC 
ATGCGTGGGG GCATCCTCTA CACAGAACCC CGGGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCTTGGTGAT TGAGTTCATC TACGCTATCG TGGGCATCGT CTGGCTCACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCTAAGAATG TCACCCTCGG AATGGTCGTC 
TGCAACTGGG TGGTCATTCT CAGCGTCTGC ATCACTGTCC TCTGCGTCTT TGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAATCT GCGGACTTAC 
AACCTGCGGC ACCGGTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG TCTAAAAGTG 
TTCCTCTGTT GCACCCGGAC AAAGGATTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCTG AATTTTTCCG TGACCTCGAC ATCGTGCCCT CCGACATCAT TGCTGGCCTG 
GTGCTGCTTA GACAGCGGCA GCGAGCCAAA CGCAATGCGG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT ACGCTACAAA GAAGTCTGCT ACTACATGCT CTTTGCCCTA 
GCCGCCTATG GCTGGCCCAT GTACCTGATG AGGAAGCCCA CCTGTGGTCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGCTG TCTCTGCCCT GCACGACCCC GATTCGCCCC TGGGGTCACC 
ATTGAGGAAG ACAACTGTTG TGGCTGCAAC GCAATCGCTA TCCGCCGTCA CTTCCTGGAT 
GAGAACATGA CTGCTGTAGA TATTGTCTAC ACCTCCTGTC ACGATGCGGT CTATGAAACT 
CCCTTCTACG TAGCTGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGAACA 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACAGGCGATG CTGAGCGCCT CCCTGTGGAG 
GGACACAGAG GCACCTGGTT GGGCCACAAG GGCATGGTAC TCTCAGCTGA GTACATCAAG 
AAGAAGTTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGTGGA 
ACCAAACACT ATGGCCTGAT TGTGGTAGGC CACTCCTTGG GAGCAGGCAC AGCCGCCATC 
CTCTCCTTCC TCCTGCGCCC CCAGTACCCG ACCCTCAAGT GCTTCGCTTA CTCCCCACCA 
GGAGGCCTGC TAAGTGAGGA TGCTATGGAA TACTCCAAAG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTCCC CAGGATTGGC CTTTCCCAGC TGGAAGGTTT CCGTCGGCAG 
CTTCTGGATG TCCTGCAGCG GAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGTATCC CCAAGTCGGA GCTGCCCGAG GATCAGGTGG AGGTGACCAC TCTGGCCAGC 
ACACGACTCT GGACACACCC CAGTGATTTG ACCATCGCCC TTTCGGCTAG CACCCCACTC 
TACCCACCTG GCCGCATCAT CCACGTGGTT CACAACCATC CTGCTGAGCA GTGCTGCTGC 
TGTGAGCAGG AGGAGCCCAC ATACTTTGCC ATCTGGGGCG ACAATAAAGC CTTTAACGAG 
GTGATCATCT CACCAGCCAT GCTGCATGAG CACCTGCCCT ATGTGGTCAT GGAGGGGCTC 
AATAAGGTGC TGGAGAATTA CAACAAAGGA AAGACAGCGC TGCTGTCTGC TGCAAAGGTC 
ATGGTGAGCC CCACCGAGGT AGACCTCACT CCCGAGCTCA TCTTCCAGCA ACAGCCGTTG 
CCCACGGGCC CACCTCTGCC CACTGGCCTG GCCCTGGAGC TGCCTGCCAC AGAGCATCGC 
AACAGCAGTG TCAGGAGCAA GTCTCAGTCT GAGATGAGTC TGGAGGGCTT CTCTGAGGGC 
CGGCTGCTGT CCCCAGTGGC TGCTGCATCA GCAGCCCGAC AAGACCCCGT AGAGCTTCTG 
CTGCTGTCCA CCCAGGAGCG ACTGGCAGCA GAGCTGCAGT CCCGGCGGGC ACCACTGGCC 
ACCATGGAGA GCCTCTCAGA CACTGAGTCA CTGTACAGCT TCGACTCACG CCGCTCCTCA 
GGCTTCCGAA GCATCAGAGG CTCGCCCAGC CTCCATGCAG TACTGGAGCG TGACGAGGGC 
CACCTGTTTT ACATCGACCC GGCCATTCCC GAGGAGAACC CGTCCCTCAG CTCACGCACA 
GAGCTGCTGG CAGCCGACAG TCTGTCCAAG CACTCACAGG ACACCCAACC CCTGGAGGCG 
GCTCTAGGCA GCGGGGGTGT GACTCCTGAG CGACCACCAA GTGCAACCAT TGAAGAGGAG 
GAGGCCGCAG GTGGCAGTGA GGGTGGTGGG GTGGCCCCCC GGGGAGAGCT GGCACTGCAC 
AATGGGCGCC TGGGGGACTC ACCCAGCCCT CAGGTGCTAG AATTCGCCGA GTTCATTGAC 
AGCCTCTTCA ACCTGGACAG CAAGAGCAGC TCCTTCCAGG ACCTCTACTG CATGATGGTG 
CCTGAGAGCC CCACTAGTGA CTACACTGAG GGCCCCAAGT CCCCCAGCCA ACAGGAAATC 
TTGCTTCGGG CTCAGTTTGA GCCCAACCTG GTACCCAAGC CCCCCAGGCT CTTTGCAGGA 
TCTGCTGAAC CCTCCTCTGG CATCTCGCTC TCACCCTCCT TCCCACTCAG CTCCTCGGGG 
GAGCTCATGG ACCTGACCCC CACGGGCCTC AGCAGCCAGG AGTGTCTGGC CACAGACAAG 
ATCCGGACTT CCACCCCCAC AGGCCACGGG GCCAGCCCCA CCAAGCAGGA TGACCTGGTC 
ATCTCGGCAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006527177.1

Translation

MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPTCGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEDQVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPLPTGLALELPATEHRNSSVRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQSRRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSATIEEEEAAGGSEGGGVAPRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMMVPESPTSDYTEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSAEPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLATDKIRTSTPTGHGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	XM_006527114.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus diacylglycerol lipase, alpha (Dagla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527114.2

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCTATCT TCCTCTTCCT GCTACACACC ACCTGGTTTG TAATCCTGTC TGTGGTGCTC 
TTCGGCTTGG TCTATAACCC ACACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGA 
GGCTACCTGG GCATCCTTCT GAGCTGTATG ATTGCCGAAA TGGCTATCAT CTGGCTGAGC 
ATGCGTGGGG GCATCCTCTA CACAGAACCC CGGGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCTTGGTGAT TGAGTTCATC TACGCTATCG TGGGCATCGT CTGGCTCACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCTAAGAATG TCACCCTCGG AATGGTCGTC 
TGCAACTGGG TGGTCATTCT CAGCGTCTGC ATCACTGTCC TCTGCGTCTT TGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAATCT GCGGACTTAC 
AACCTGCGGC ACCGGTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG TCTAAAAGTG 
TTCCTCTGTT GCACCCGGAC AAAGGATTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCTG AATTTTTCCG TGACCTCGAC ATCGTGCCCT CCGACATCAT TGCTGGCCTG 
GTGCTGCTTA GACAGCGGCA GCGAGCCAAA CGCAATGCGG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT ACGCTACAAA GAAGTCTGCT ACTACATGCT CTTTGCCCTA 
GCCGCCTATG GCTGGCCCAT GTACCTGATG AGGAAGCCCA CCTGTGGTCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGCTG TCTCTGCCCT GCACGACCCC GATTCGCCCC TGGGGTCACC 
ATTGAGGAAG ACAACTGTTG TGGCTGCAAC GCAATCGCTA TCCGCCGTCA CTTCCTGGAT 
GAGAACATGA CTGCTGTAGA TATTGTCTAC ACCTCCTGTC ACGATGCGGT CTATGAAACT 
CCCTTCTACG TAGCTGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGAACA 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACAGGCGATG CTGAGCGCCT CCCTGTGGAG 
GGACACAGAG GCACCTGGTT GGGCCACAAG GGCATGGTAC TCTCAGCTGA GTACATCAAG 
AAGAAGTTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGTGGA 
ACCAAACACT ATGGCCTGAT TGTGGTAGGC CACTCCTTGG GAGCAGGCAC AGCCGCCATC 
CTCTCCTTCC TCCTGCGCCC CCAGTACCCG ACCCTCAAGT GCTTCGCTTA CTCCCCACCA 
GGAGGCCTGC TAAGTGAGGA TGCTATGGAA TACTCCAAAG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTCCC CAGGATTGGC CTTTCCCAGC TGGAAGGTTT CCGTCGGCAG 
CTTCTGGATG TCCTGCAGCG GAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGTATCC CCAAGTCGGA GCTGCCCGAG GATCAGGTGG AGGTGACCAC TCTGGCCAGC 
ACACGACTCT GGACACACCC CAGTGATTTG ACCATCGCCC TTTCGGCTAG CACCCCACTC 
TACCCACCTG GCCGCATCAT CCACGTGGTT CACAACCATC CTGCTGAGCA GTGCTGCTGC 
TGTGAGCAGG AGGAGCCCAC ATACTTTGCC ATCTGGGGCG ACAATAAAGC CTTTAACGAG 
GTGATCATCT CACCAGCCAT GCTGCATGAG CACCTGCCCT ATGTGGTCAT GGAGGGGCTC 
AATAAGGTGC TGGAGAATTA CAACAAAGGA AAGACAGCGC TGCTGTCTGC TGCAAAGGTC 
ATGGTGAGCC CCACCGAGGT AGACCTCACT CCCGAGCTCA TCTTCCAGCA ACAGCCGTTG 
CCCACGGGCC CACCTCTGCC CACTGGCCTG GCCCTGGAGC TGCCTGCCAC AGAGCATCGC 
AACAGCAGTG TCAGGAGCAA GTCTCAGTCT GAGATGAGTC TGGAGGGCTT CTCTGAGGGC 
CGGCTGCTGT CCCCAGTGGC TGCTGCATCA GCAGCCCGAC AAGACCCCGT AGAGCTTCTG 
CTGCTGTCCA CCCAGGAGCG ACTGGCAGCA GAGCTGCAGT CCCGGCGGGC ACCACTGGCC 
ACCATGGAGA GCCTCTCAGA CACTGAGTCA CTGTACAGCT TCGACTCACG CCGCTCCTCA 
GGCTTCCGAA GCATCAGAGG CTCGCCCAGC CTCCATGCAG TACTGGAGCG TGACGAGGGC 
CACCTGTTTT ACATCGACCC GGCCATTCCC GAGGAGAACC CGTCCCTCAG CTCACGCACA 
GAGCTGCTGG CAGCCGACAG TCTGTCCAAG CACTCACAGG ACACCCAACC CCTGGAGGCG 
GCTCTAGGCA GCGGGGGTGT GACTCCTGAG CGACCACCAA GTGCAACCAT TGAAGAGGAG 
GAGGCCGCAG GTGGCAGTGA GGGTGGTGGG GTGGCCCCCC GGGGAGAGCT GGCACTGCAC 
AATGGGCGCC TGGGGGACTC ACCCAGCCCT CAGGTGCTAG AATTCGCCGA GTTCATTGAC 
AGCCTCTTCA ACCTGGACAG CAAGAGCAGC TCCTTCCAGG ACCTCTACTG CATGATGGTG 
CCTGAGAGCC CCACTAGTGA CTACACTGAG GGCCCCAAGT CCCCCAGCCA ACAGGAAATC 
TTGCTTCGGG CTCAGTTTGA GCCCAACCTG GTACCCAAGC CCCCCAGGCT CTTTGCAGGA 
TCTGCTGAAC CCTCCTCTGG CATCTCGCTC TCACCCTCCT TCCCACTCAG CTCCTCGGGG 
GAGCTCATGG ACCTGACCCC CACGGGCCTC AGCAGCCAGG AGTGTCTGGC CACAGACAAG 
ATCCGGACTT CCACCCCCAC AGGCCACGGG GCCAGCCCCA CCAAGCAGGA TGACCTGGTC 
ATCTCGGCAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crucial Roles of the Endocannabinoid 2-Arachidonoylglycerol in the Suppression of Epileptic Seizures.
Cell reports16(5)1405-1415(2016 08)
Sugaya Y,Yamazaki M,Uchigashima M,Kobayashi K,Watanabe M,Sakimura K,Kano M

Myeloid-Specific Deletion of Diacylglycerol Lipase ? Inhibits Atherogenesis in ApoE-Deficient Mice.
PloS one11(1)e0146267(2016)
Jehle J,Hoyer FF,Sch?ne B,Pfeifer P,Schild K,Jenniches I,Bindila L,Lutz B,L?tjohann D,Zimmer A,Nickenig G

Rapid and profound rewiring of brain lipid signaling networks by acute diacylglycerol lipase inhibition.
Proceedings of the National Academy of Sciences of the United States of America113(1)26-33(2016 Jan)
Ogasawara D,Deng H,Viader A,Baggelaar MP,Breman A,den Dulk H,van den Nieuwendijk AM,van den Nieuwendijk AM,Soethoudt M,van der Wel T,Zhou J,Overkleeft HS,Sanchez-Alavez M,Mori S,Mo S,Nguyen W,Conti B,Liu X,Chen Y,Liu QS,Cravatt BF,van der Stelt M

A chemical proteomic atlas of brain serine hydrolases identifies cell type-specific pathways regulating neuroinflammation.
eLife5e12345(2016 Jan)
Viader A,Ogasawara D,Joslyn CM,Sanchez-Alavez M,Mori S,Nguyen W,Conti B,Cravatt BF

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DAGLA cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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