DAGLA cDNA ORF clone, Homo sapiens(human)

The following DAGLA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAGLA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu12075 NM_006133.3
Latest version!
Homo sapiens diacylglycerol lipase alpha (DAGLA), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu12075 XM_011545235.1
Latest version!
Homo sapiens diacylglycerol lipase, alpha (DAGLA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu12075 XM_005274230.3
Latest version!
Homo sapiens diacylglycerol lipase, alpha (DAGLA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu92478 XM_017018240.1
Latest version!
Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu92477 XM_017018239.1
Latest version!
Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu92477 XM_017018238.1
Latest version!
Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
View Old Accession Versions >>
OHu12075 NM_006133.2 Homo sapiens diacylglycerol lipase alpha (DAGLA), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu12075
    Clone ID Related Accession (Same CDS sequence) NM_006133.2 , XM_005274230.3 , XM_011545235.1 , NM_006133.3
    Accession Version NM_006133.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-31
    Organism Homo sapiens(human)
    Product sn1-specific diacylglycerol lipase alpha
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC150176.1, AP002380.3 and AI702626.1. On Nov 25, 2010 this sequence version replaced NM_006133.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC150176.1, BC152453.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
    CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC
    TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG
    TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC
    CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT
    GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG
    GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG
    GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC
    ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC
    GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG
    CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC
    CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG
    GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG
    AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC
    CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA
    GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC
    AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC
    CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT
    GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG
    ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG
    GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC
    ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC
    AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG
    CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG
    TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG
    GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC
    CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC
    TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG
    CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG
    GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA
    GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG
    CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC
    TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG
    AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC
    CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC
    GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC
    ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG
    ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA
    GCACGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006124.1
    CDS117..3245
    Translation

    Target ORF information:

    RefSeq Version NM_006133.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol lipase alpha (DAGLA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006133.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
    CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC
    TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG
    TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC
    CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT
    GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG
    GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG
    GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC
    ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC
    GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG
    CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC
    CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG
    GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG
    AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC
    CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA
    GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC
    AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC
    CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT
    GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG
    ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG
    GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC
    ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC
    AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG
    CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG
    TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG
    GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC
    CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC
    TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG
    CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG
    GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA
    GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG
    CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC
    TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG
    AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC
    CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC
    GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC
    ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG
    ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA
    GCACGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12075
    Clone ID Related Accession (Same CDS sequence) NM_006133.2 , XM_005274230.3 , XM_011545235.1 , NM_006133.3
    Accession Version NM_006133.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product sn1-specific diacylglycerol lipase alpha
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP002380.3 and BC150176.1. On Nov 22, 2018 this sequence version replaced NM_006133.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150176.1, BC152453.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000257215.10/ ENSP00000257215.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
    CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC
    TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG
    TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC
    CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT
    GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG
    GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG
    GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC
    ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC
    GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG
    CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC
    CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG
    GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG
    AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC
    CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA
    GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC
    AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC
    CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT
    GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG
    ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG
    GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC
    ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC
    AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG
    CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG
    TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG
    GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC
    CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC
    TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG
    CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG
    GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA
    GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG
    CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC
    TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG
    AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC
    CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC
    GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC
    ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG
    ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA
    GCACGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006124.1
    CDS159..3287
    Translation

    Target ORF information:

    RefSeq Version NM_006133.3
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol lipase alpha (DAGLA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006133.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
    CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC
    TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG
    TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC
    CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT
    GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG
    GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG
    GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC
    ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC
    GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG
    CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC
    CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG
    GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG
    AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC
    CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA
    GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC
    AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC
    CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT
    GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG
    ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG
    GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC
    ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC
    AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG
    CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG
    TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG
    GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC
    CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC
    TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG
    CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG
    GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA
    GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG
    CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC
    TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG
    AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC
    CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC
    GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC
    ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG
    ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA
    GCACGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12075
    Clone ID Related Accession (Same CDS sequence) NM_006133.2 , XM_005274230.3 , XM_011545235.1 , NM_006133.3
    Accession Version XM_005274230.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product sn1-specific diacylglycerol lipase alpha isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
    CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC
    TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG
    TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC
    CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT
    GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG
    GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG
    GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC
    ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC
    GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG
    CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC
    CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG
    GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG
    AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC
    CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA
    GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC
    AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC
    CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT
    GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG
    ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG
    GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC
    ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC
    AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG
    CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG
    TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG
    GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC
    CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC
    TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG
    CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG
    GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA
    GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG
    CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC
    TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG
    AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC
    CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC
    GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC
    ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG
    ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA
    GCACGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005274287.1
    Translation

    Target ORF information:

    RefSeq Version XM_005274230.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens diacylglycerol lipase, alpha (DAGLA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005274230.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
    CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC
    TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG
    TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC
    CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT
    GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG
    GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG
    GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC
    ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC
    GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG
    CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC
    CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG
    GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG
    AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC
    CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA
    GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC
    AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC
    CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT
    GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG
    ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG
    GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC
    ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC
    AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG
    CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG
    TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG
    GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC
    CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC
    TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG
    CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG
    GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA
    GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG
    CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC
    TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG
    AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC
    CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC
    GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC
    ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG
    ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA
    GCACGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12075
    Clone ID Related Accession (Same CDS sequence) NM_006133.2 , XM_005274230.3 , XM_011545235.1 , NM_006133.3
    Accession Version XM_011545235.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product sn1-specific diacylglycerol lipase alpha isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
    CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC
    TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG
    TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC
    CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT
    GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG
    GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG
    GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC
    ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC
    GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG
    CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC
    CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG
    GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG
    AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC
    CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA
    GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC
    AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC
    CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT
    GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG
    ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG
    GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC
    ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC
    AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG
    CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG
    TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG
    GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC
    CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC
    TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG
    CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG
    GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA
    GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG
    CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC
    TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG
    AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC
    CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC
    GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC
    ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG
    ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA
    GCACGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543537.1
    Translation

    Target ORF information:

    RefSeq Version XM_011545235.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens diacylglycerol lipase, alpha (DAGLA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545235.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
    CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC
    TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG
    TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC
    CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT
    GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG
    GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG
    GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC
    ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC
    GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG
    CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC
    CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG
    GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG
    AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC
    CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA
    GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC
    AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC
    CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT
    GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG
    ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG
    GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC
    ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC
    AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG
    CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG
    TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG
    GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC
    CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC
    TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG
    CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG
    GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA
    GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG
    CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC
    TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG
    AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC
    CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC
    GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC
    ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG
    ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA
    GCACGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92478
    Clone ID Related Accession (Same CDS sequence) XM_017018240.1
    Accession Version XM_017018240.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sn1-specific diacylglycerol lipase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
    CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC
    TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG
    TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC
    CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT
    GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAATTCAA CAAATCCCCT GAATTCCTTT ACCAAGTTCA GAAGTGTCTG GTCCTGGCCT
    CTTGAGATGA CAGAGCACTT TACCAGGCAA GAGATGCTCC GCTACAAAGA GGTCTGCTAC
    TACATGCTCT TTGCCCTGGC TGCCTACGGG TGGCCCATGT ACCTGATGCG GAAGCCCGCC
    TGCGGCCTCT GCCAACTGGC TCGGTCCTGC TCGTGTTGCC TGTGTCCTGC GAGGCCGCGG
    TTCGCCCCTG GAGTCACCAT CGAGGAAGAC AACTGCTGTG GCTGTAATGC CATTGCCATC
    CGGCGCCACT TCCTGGACGA GAACATGACT GCGGTGGACA TCGTCTATAC CTCCTGCCAT
    GATGCGGTCT ATGAAACGCC CTTCTACGTG GCGGTGGACC ATGACAAGAA GAAAGTGGTG
    ATCAGTATCC GGGGGACCCT GTCCCCCAAG GATGCCCTGA CTGACCTGAC GGGTGATGCT
    GAGCGCCTCC CCGTGGAGGG GCACCACGGC ACCTGGCTGG GCCACAAGGG TATGGTCCTC
    TCAGCTGAGT ACATCAAGAA GAAACTGGAG CAGGAGATGG TCCTGTCCCA GGCCTTTGGG
    CGAGACCTGG GCCGCGGAAC CAAACACTAC GGCCTGATTG TGGTGGGCCA CTCCCTGGGC
    GCGGGCACTG CTGCCATCCT CTCCTTCCTT CTGCGCCCAC AGTATCCGAC CCTCAAGTGC
    TTTGCCTACT CCCCGCCAGG GGGCCTGCTG AGTGAGGATG CGATGGAGTA TTCCAAGGAG
    TTCGTGACTG CTGTGGTTCT GGGCAAAGAC CTCGTCCCCA GGATTGGCCT CTCTCAGCTG
    GAAGGCTTCC GCAGACAGCT CCTGGATGTC CTGCAGCGAA GCACCAAGCC CAAAATGAGG
    GGTTGGCTGG GCTTCCAGCC CCTTGCCAGC CCTGAAATTC AACAATTCCA TGTCATCTGT
    CCTTGGCACC TTATTGGCCG TCTGGCCACC GTGCACCTCA GCCACTGCCT CTTCAGTGGC
    GGATCATCGT GGGGGCCACC AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873729.1
    CDS366..2282
    Translation

    Target ORF information:

    RefSeq Version XM_017018240.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018240.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
    CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC
    TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG
    TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC
    CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT
    GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAATTCAA CAAATCCCCT GAATTCCTTT ACCAAGTTCA GAAGTGTCTG GTCCTGGCCT
    CTTGAGATGA CAGAGCACTT TACCAGGCAA GAGATGCTCC GCTACAAAGA GGTCTGCTAC
    TACATGCTCT TTGCCCTGGC TGCCTACGGG TGGCCCATGT ACCTGATGCG GAAGCCCGCC
    TGCGGCCTCT GCCAACTGGC TCGGTCCTGC TCGTGTTGCC TGTGTCCTGC GAGGCCGCGG
    TTCGCCCCTG GAGTCACCAT CGAGGAAGAC AACTGCTGTG GCTGTAATGC CATTGCCATC
    CGGCGCCACT TCCTGGACGA GAACATGACT GCGGTGGACA TCGTCTATAC CTCCTGCCAT
    GATGCGGTCT ATGAAACGCC CTTCTACGTG GCGGTGGACC ATGACAAGAA GAAAGTGGTG
    ATCAGTATCC GGGGGACCCT GTCCCCCAAG GATGCCCTGA CTGACCTGAC GGGTGATGCT
    GAGCGCCTCC CCGTGGAGGG GCACCACGGC ACCTGGCTGG GCCACAAGGG TATGGTCCTC
    TCAGCTGAGT ACATCAAGAA GAAACTGGAG CAGGAGATGG TCCTGTCCCA GGCCTTTGGG
    CGAGACCTGG GCCGCGGAAC CAAACACTAC GGCCTGATTG TGGTGGGCCA CTCCCTGGGC
    GCGGGCACTG CTGCCATCCT CTCCTTCCTT CTGCGCCCAC AGTATCCGAC CCTCAAGTGC
    TTTGCCTACT CCCCGCCAGG GGGCCTGCTG AGTGAGGATG CGATGGAGTA TTCCAAGGAG
    TTCGTGACTG CTGTGGTTCT GGGCAAAGAC CTCGTCCCCA GGATTGGCCT CTCTCAGCTG
    GAAGGCTTCC GCAGACAGCT CCTGGATGTC CTGCAGCGAA GCACCAAGCC CAAAATGAGG
    GGTTGGCTGG GCTTCCAGCC CCTTGCCAGC CCTGAAATTC AACAATTCCA TGTCATCTGT
    CCTTGGCACC TTATTGGCCG TCTGGCCACC GTGCACCTCA GCCACTGCCT CTTCAGTGGC
    GGATCATCGT GGGGGCCACC AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92477
    Clone ID Related Accession (Same CDS sequence) XM_017018239.1 , XM_017018238.1
    Accession Version XM_017018239.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sn1-specific diacylglycerol lipase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
    CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC
    TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG
    TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC
    CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT
    GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAATTCAA CAAATCCCCT GAATTCCTTT ACCAAGTTCA GAAGTGTCTG GTCCTGGCCT
    CTTGAGATGA CAGAGCACTT TACCAGGCAA GAGATGCTCC GCTACAAAGA GGTCTGCTAC
    TACATGCTCT TTGCCCTGGC TGCCTACGGG TGGCCCATGT ACCTGATGCG GAAGCCCGCC
    TGCGGCCTCT GCCAACTGGC TCGGTCCTGC TCGTGTTGCC TGTGTCCTGC GAGGCCGCGG
    TTCGCCCCTG GAGTCACCAT CGAGGAAGAC AACTGCTGTG GCTGTAATGC CATTGCCATC
    CGGCGCCACT TCCTGGACGA GAACATGACT GCGGTGGACA TCGTCTATAC CTCCTGCCAT
    GATGCGGTCT ATGAAACGCC CTTCTACGTG GCGGTGGACC ATGACAAGAA GAAAGTGGTG
    ATCAGTATCC GGGGGACCCT GTCCCCCAAG GATGCCCTGA CTGACCTGAC GGGTGATGCT
    GAGCGCCTCC CCGTGGAGGG GCACCACGGC ACCTGGCTGG GCCACAAGGG TATGGTCCTC
    TCAGCTGAGT ACATCAAGAA GAAACTGGAG CAGGAGATGG TCCTGTCCCA GGCCTTTGGG
    CGAGACCTGG GCCGCGGAAC CAAACACTAC GGCCTGATTG TGGTGGGCCA CTCCCTGGGC
    GCGGGCACTG CTGCCATCCT CTCCTTCCTT CTGCGCCCAC AGTATCCGAC CCTCAAGTGC
    TTTGCCTACT CCCCGCCAGG GGGCCTGCTG AGTGAGGATG CGATGGAGTA TTCCAAGGAG
    TTCGTGACTG CTGTGGTTCT GGGCAAAGAC CTCGTCCCCA GGATTGGCCT CTCTCAGCTG
    GAAGGCTTCC GCAGACAGCT CCTGGATGTC CTGCAGCGAA GCACCAAGCC CAAATGGCGG
    ATCATCGTGG GGGCCACCAA ATGCATCCCC AAGTCGGAGC TGCCTGAGGA GGTAGAGGTG
    ACCACCCTGG CCAGCACGCG GCTCTGGACC CACCCCAGCG ACCTAACTAT AGCCCTCTCA
    GCCAGCACTC CACTCTACCC GCCCGGCCGC ATCATCCACG TGGTCCACAA CCACCCTGCA
    GAGCAGTGCT GCTGCTGTGA GCAGGAGGAG CCCACATACT TTGCCATCTG GGGCGACAAC
    AAGGCCTTCA ATGAGGTGAT CATCTCGCCA GCCATGCTGC ATGAGCACCT GCCCTATGTG
    GTCATGGAGG GGCTCAACAA GGTGCTGGAG AACTACAACA AGGGGAAGAC CGCTCTGCTC
    TCTGCAGCCA AGGTCATGGT GAGCCCTACC GAGGTGGACC TGACTCCTGA GCTCATCTTC
    CAGCAGCAGC CACTCCCCAC GGGGCCGCCC ATGCCCACTG GCCTTGCCCT GGAGCTGCCG
    ACTGCAGACC ACCGCAACAG CAGCGTCAGG AGCAAGTCCC AGTCTGAGAT GAGCCTGGAG
    GGCTTCTCGG AGGGGCGGCT GCTGTCGCCA GTGGTTGCGG CGGCGGCCCG CCAGGACCCG
    GTGGAGCTGC TGCTGCTGTC TACCCAGGAG CGGCTGGCGG CGGAGCTGCA GGCCCGGCGG
    GCACCACTGG CCACCATGGA GAGCCTCTCG GACACTGAGT CCCTGTACAG CTTCGACTCG
    CGCCGCTCCT CAGGCTTCCG CAGCATCCGG GGCTCCCCCA GCCTCCACGC TGTGCTGGAG
    CGTGATGAAG GCCACCTCTT CTACATTGAC CCTGCCATCC CCGAGGAAAA CCCATCCCTG
    AGCTCGCGCA CTGAGCTGCT GGCGGCCGAC AGCCTGTCCA AGCACTCACA GGACACGCAG
    CCCCTGGAGG CGGCCCTGGG CAGTGGCGGC GTCACTCCTG AGCGGCCCCC CAGTGCTGCG
    GCCAATGACG AGGAGGAAGA GGTTGGCGGT GGGGGTGGCG GGCCGGCCTC CCGCGGGGAG
    CTGGCGCTGC ACAATGGGCG CCTGGGGGAC TCGCCCAGTC CTCAGGTGCT GGAATTCGCC
    GAGTTCATCG ACAGCCTCTT CAACCTGGAC AGCAAGAGCA GCTCCTTCCA AGACCTCTAC
    TGCATGGTGG TGCCCGAGAG CCCCACCAGT GACTACGCTG AGGGCCCCAA GTCCCCCAGC
    CAGCAAGAGA TCCTGCTCCG TGCCCAGTTC GAGCCCAACC TGGTGCCCAA GCCCCCACGG
    CTCTTTGCCG GCTCAGCCGA CCCCTCCTCG GGCATCTCAC TCTCGCCCTC CTTCCCGCTC
    AGCTCCTCGG GTGAGCTCAT GGACCTGACG CCCACGGGCC TCAGTAGCCA GGAATGCCTG
    GCGGCTGACA AGATCCGGAC TTCTACCCCC ACTGGCCACG GAGCCAGCCC CGCCAAGCAA
    GATGAGCTGG TCATCTCAGC ACGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873728.1
    CDS271..3477
    Translation

    Target ORF information:

    RefSeq Version XM_017018239.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018239.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
    CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC
    TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG
    TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC
    CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT
    GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAATTCAA CAAATCCCCT GAATTCCTTT ACCAAGTTCA GAAGTGTCTG GTCCTGGCCT
    CTTGAGATGA CAGAGCACTT TACCAGGCAA GAGATGCTCC GCTACAAAGA GGTCTGCTAC
    TACATGCTCT TTGCCCTGGC TGCCTACGGG TGGCCCATGT ACCTGATGCG GAAGCCCGCC
    TGCGGCCTCT GCCAACTGGC TCGGTCCTGC TCGTGTTGCC TGTGTCCTGC GAGGCCGCGG
    TTCGCCCCTG GAGTCACCAT CGAGGAAGAC AACTGCTGTG GCTGTAATGC CATTGCCATC
    CGGCGCCACT TCCTGGACGA GAACATGACT GCGGTGGACA TCGTCTATAC CTCCTGCCAT
    GATGCGGTCT ATGAAACGCC CTTCTACGTG GCGGTGGACC ATGACAAGAA GAAAGTGGTG
    ATCAGTATCC GGGGGACCCT GTCCCCCAAG GATGCCCTGA CTGACCTGAC GGGTGATGCT
    GAGCGCCTCC CCGTGGAGGG GCACCACGGC ACCTGGCTGG GCCACAAGGG TATGGTCCTC
    TCAGCTGAGT ACATCAAGAA GAAACTGGAG CAGGAGATGG TCCTGTCCCA GGCCTTTGGG
    CGAGACCTGG GCCGCGGAAC CAAACACTAC GGCCTGATTG TGGTGGGCCA CTCCCTGGGC
    GCGGGCACTG CTGCCATCCT CTCCTTCCTT CTGCGCCCAC AGTATCCGAC CCTCAAGTGC
    TTTGCCTACT CCCCGCCAGG GGGCCTGCTG AGTGAGGATG CGATGGAGTA TTCCAAGGAG
    TTCGTGACTG CTGTGGTTCT GGGCAAAGAC CTCGTCCCCA GGATTGGCCT CTCTCAGCTG
    GAAGGCTTCC GCAGACAGCT CCTGGATGTC CTGCAGCGAA GCACCAAGCC CAAATGGCGG
    ATCATCGTGG GGGCCACCAA ATGCATCCCC AAGTCGGAGC TGCCTGAGGA GGTAGAGGTG
    ACCACCCTGG CCAGCACGCG GCTCTGGACC CACCCCAGCG ACCTAACTAT AGCCCTCTCA
    GCCAGCACTC CACTCTACCC GCCCGGCCGC ATCATCCACG TGGTCCACAA CCACCCTGCA
    GAGCAGTGCT GCTGCTGTGA GCAGGAGGAG CCCACATACT TTGCCATCTG GGGCGACAAC
    AAGGCCTTCA ATGAGGTGAT CATCTCGCCA GCCATGCTGC ATGAGCACCT GCCCTATGTG
    GTCATGGAGG GGCTCAACAA GGTGCTGGAG AACTACAACA AGGGGAAGAC CGCTCTGCTC
    TCTGCAGCCA AGGTCATGGT GAGCCCTACC GAGGTGGACC TGACTCCTGA GCTCATCTTC
    CAGCAGCAGC CACTCCCCAC GGGGCCGCCC ATGCCCACTG GCCTTGCCCT GGAGCTGCCG
    ACTGCAGACC ACCGCAACAG CAGCGTCAGG AGCAAGTCCC AGTCTGAGAT GAGCCTGGAG
    GGCTTCTCGG AGGGGCGGCT GCTGTCGCCA GTGGTTGCGG CGGCGGCCCG CCAGGACCCG
    GTGGAGCTGC TGCTGCTGTC TACCCAGGAG CGGCTGGCGG CGGAGCTGCA GGCCCGGCGG
    GCACCACTGG CCACCATGGA GAGCCTCTCG GACACTGAGT CCCTGTACAG CTTCGACTCG
    CGCCGCTCCT CAGGCTTCCG CAGCATCCGG GGCTCCCCCA GCCTCCACGC TGTGCTGGAG
    CGTGATGAAG GCCACCTCTT CTACATTGAC CCTGCCATCC CCGAGGAAAA CCCATCCCTG
    AGCTCGCGCA CTGAGCTGCT GGCGGCCGAC AGCCTGTCCA AGCACTCACA GGACACGCAG
    CCCCTGGAGG CGGCCCTGGG CAGTGGCGGC GTCACTCCTG AGCGGCCCCC CAGTGCTGCG
    GCCAATGACG AGGAGGAAGA GGTTGGCGGT GGGGGTGGCG GGCCGGCCTC CCGCGGGGAG
    CTGGCGCTGC ACAATGGGCG CCTGGGGGAC TCGCCCAGTC CTCAGGTGCT GGAATTCGCC
    GAGTTCATCG ACAGCCTCTT CAACCTGGAC AGCAAGAGCA GCTCCTTCCA AGACCTCTAC
    TGCATGGTGG TGCCCGAGAG CCCCACCAGT GACTACGCTG AGGGCCCCAA GTCCCCCAGC
    CAGCAAGAGA TCCTGCTCCG TGCCCAGTTC GAGCCCAACC TGGTGCCCAA GCCCCCACGG
    CTCTTTGCCG GCTCAGCCGA CCCCTCCTCG GGCATCTCAC TCTCGCCCTC CTTCCCGCTC
    AGCTCCTCGG GTGAGCTCAT GGACCTGACG CCCACGGGCC TCAGTAGCCA GGAATGCCTG
    GCGGCTGACA AGATCCGGAC TTCTACCCCC ACTGGCCACG GAGCCAGCCC CGCCAAGCAA
    GATGAGCTGG TCATCTCAGC ACGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92477
    Clone ID Related Accession (Same CDS sequence) XM_017018239.1 , XM_017018238.1
    Accession Version XM_017018238.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sn1-specific diacylglycerol lipase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
    CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC
    TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG
    TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC
    CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT
    GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAATTCAA CAAATCCCCT GAATTCCTTT ACCAAGTTCA GAAGTGTCTG GTCCTGGCCT
    CTTGAGATGA CAGAGCACTT TACCAGGCAA GAGATGCTCC GCTACAAAGA GGTCTGCTAC
    TACATGCTCT TTGCCCTGGC TGCCTACGGG TGGCCCATGT ACCTGATGCG GAAGCCCGCC
    TGCGGCCTCT GCCAACTGGC TCGGTCCTGC TCGTGTTGCC TGTGTCCTGC GAGGCCGCGG
    TTCGCCCCTG GAGTCACCAT CGAGGAAGAC AACTGCTGTG GCTGTAATGC CATTGCCATC
    CGGCGCCACT TCCTGGACGA GAACATGACT GCGGTGGACA TCGTCTATAC CTCCTGCCAT
    GATGCGGTCT ATGAAACGCC CTTCTACGTG GCGGTGGACC ATGACAAGAA GAAAGTGGTG
    ATCAGTATCC GGGGGACCCT GTCCCCCAAG GATGCCCTGA CTGACCTGAC GGGTGATGCT
    GAGCGCCTCC CCGTGGAGGG GCACCACGGC ACCTGGCTGG GCCACAAGGG TATGGTCCTC
    TCAGCTGAGT ACATCAAGAA GAAACTGGAG CAGGAGATGG TCCTGTCCCA GGCCTTTGGG
    CGAGACCTGG GCCGCGGAAC CAAACACTAC GGCCTGATTG TGGTGGGCCA CTCCCTGGGC
    GCGGGCACTG CTGCCATCCT CTCCTTCCTT CTGCGCCCAC AGTATCCGAC CCTCAAGTGC
    TTTGCCTACT CCCCGCCAGG GGGCCTGCTG AGTGAGGATG CGATGGAGTA TTCCAAGGAG
    TTCGTGACTG CTGTGGTTCT GGGCAAAGAC CTCGTCCCCA GGATTGGCCT CTCTCAGCTG
    GAAGGCTTCC GCAGACAGCT CCTGGATGTC CTGCAGCGAA GCACCAAGCC CAAATGGCGG
    ATCATCGTGG GGGCCACCAA ATGCATCCCC AAGTCGGAGC TGCCTGAGGA GGTAGAGGTG
    ACCACCCTGG CCAGCACGCG GCTCTGGACC CACCCCAGCG ACCTAACTAT AGCCCTCTCA
    GCCAGCACTC CACTCTACCC GCCCGGCCGC ATCATCCACG TGGTCCACAA CCACCCTGCA
    GAGCAGTGCT GCTGCTGTGA GCAGGAGGAG CCCACATACT TTGCCATCTG GGGCGACAAC
    AAGGCCTTCA ATGAGGTGAT CATCTCGCCA GCCATGCTGC ATGAGCACCT GCCCTATGTG
    GTCATGGAGG GGCTCAACAA GGTGCTGGAG AACTACAACA AGGGGAAGAC CGCTCTGCTC
    TCTGCAGCCA AGGTCATGGT GAGCCCTACC GAGGTGGACC TGACTCCTGA GCTCATCTTC
    CAGCAGCAGC CACTCCCCAC GGGGCCGCCC ATGCCCACTG GCCTTGCCCT GGAGCTGCCG
    ACTGCAGACC ACCGCAACAG CAGCGTCAGG AGCAAGTCCC AGTCTGAGAT GAGCCTGGAG
    GGCTTCTCGG AGGGGCGGCT GCTGTCGCCA GTGGTTGCGG CGGCGGCCCG CCAGGACCCG
    GTGGAGCTGC TGCTGCTGTC TACCCAGGAG CGGCTGGCGG CGGAGCTGCA GGCCCGGCGG
    GCACCACTGG CCACCATGGA GAGCCTCTCG GACACTGAGT CCCTGTACAG CTTCGACTCG
    CGCCGCTCCT CAGGCTTCCG CAGCATCCGG GGCTCCCCCA GCCTCCACGC TGTGCTGGAG
    CGTGATGAAG GCCACCTCTT CTACATTGAC CCTGCCATCC CCGAGGAAAA CCCATCCCTG
    AGCTCGCGCA CTGAGCTGCT GGCGGCCGAC AGCCTGTCCA AGCACTCACA GGACACGCAG
    CCCCTGGAGG CGGCCCTGGG CAGTGGCGGC GTCACTCCTG AGCGGCCCCC CAGTGCTGCG
    GCCAATGACG AGGAGGAAGA GGTTGGCGGT GGGGGTGGCG GGCCGGCCTC CCGCGGGGAG
    CTGGCGCTGC ACAATGGGCG CCTGGGGGAC TCGCCCAGTC CTCAGGTGCT GGAATTCGCC
    GAGTTCATCG ACAGCCTCTT CAACCTGGAC AGCAAGAGCA GCTCCTTCCA AGACCTCTAC
    TGCATGGTGG TGCCCGAGAG CCCCACCAGT GACTACGCTG AGGGCCCCAA GTCCCCCAGC
    CAGCAAGAGA TCCTGCTCCG TGCCCAGTTC GAGCCCAACC TGGTGCCCAA GCCCCCACGG
    CTCTTTGCCG GCTCAGCCGA CCCCTCCTCG GGCATCTCAC TCTCGCCCTC CTTCCCGCTC
    AGCTCCTCGG GTGAGCTCAT GGACCTGACG CCCACGGGCC TCAGTAGCCA GGAATGCCTG
    GCGGCTGACA AGATCCGGAC TTCTACCCCC ACTGGCCACG GAGCCAGCCC CGCCAAGCAA
    GATGAGCTGG TCATCTCAGC ACGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873727.1
    CDS366..3572
    Translation

    Target ORF information:

    RefSeq Version XM_017018238.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018238.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
    CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC
    TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG
    TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC
    CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT
    GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAATTCAA CAAATCCCCT GAATTCCTTT ACCAAGTTCA GAAGTGTCTG GTCCTGGCCT
    CTTGAGATGA CAGAGCACTT TACCAGGCAA GAGATGCTCC GCTACAAAGA GGTCTGCTAC
    TACATGCTCT TTGCCCTGGC TGCCTACGGG TGGCCCATGT ACCTGATGCG GAAGCCCGCC
    TGCGGCCTCT GCCAACTGGC TCGGTCCTGC TCGTGTTGCC TGTGTCCTGC GAGGCCGCGG
    TTCGCCCCTG GAGTCACCAT CGAGGAAGAC AACTGCTGTG GCTGTAATGC CATTGCCATC
    CGGCGCCACT TCCTGGACGA GAACATGACT GCGGTGGACA TCGTCTATAC CTCCTGCCAT
    GATGCGGTCT ATGAAACGCC CTTCTACGTG GCGGTGGACC ATGACAAGAA GAAAGTGGTG
    ATCAGTATCC GGGGGACCCT GTCCCCCAAG GATGCCCTGA CTGACCTGAC GGGTGATGCT
    GAGCGCCTCC CCGTGGAGGG GCACCACGGC ACCTGGCTGG GCCACAAGGG TATGGTCCTC
    TCAGCTGAGT ACATCAAGAA GAAACTGGAG CAGGAGATGG TCCTGTCCCA GGCCTTTGGG
    CGAGACCTGG GCCGCGGAAC CAAACACTAC GGCCTGATTG TGGTGGGCCA CTCCCTGGGC
    GCGGGCACTG CTGCCATCCT CTCCTTCCTT CTGCGCCCAC AGTATCCGAC CCTCAAGTGC
    TTTGCCTACT CCCCGCCAGG GGGCCTGCTG AGTGAGGATG CGATGGAGTA TTCCAAGGAG
    TTCGTGACTG CTGTGGTTCT GGGCAAAGAC CTCGTCCCCA GGATTGGCCT CTCTCAGCTG
    GAAGGCTTCC GCAGACAGCT CCTGGATGTC CTGCAGCGAA GCACCAAGCC CAAATGGCGG
    ATCATCGTGG GGGCCACCAA ATGCATCCCC AAGTCGGAGC TGCCTGAGGA GGTAGAGGTG
    ACCACCCTGG CCAGCACGCG GCTCTGGACC CACCCCAGCG ACCTAACTAT AGCCCTCTCA
    GCCAGCACTC CACTCTACCC GCCCGGCCGC ATCATCCACG TGGTCCACAA CCACCCTGCA
    GAGCAGTGCT GCTGCTGTGA GCAGGAGGAG CCCACATACT TTGCCATCTG GGGCGACAAC
    AAGGCCTTCA ATGAGGTGAT CATCTCGCCA GCCATGCTGC ATGAGCACCT GCCCTATGTG
    GTCATGGAGG GGCTCAACAA GGTGCTGGAG AACTACAACA AGGGGAAGAC CGCTCTGCTC
    TCTGCAGCCA AGGTCATGGT GAGCCCTACC GAGGTGGACC TGACTCCTGA GCTCATCTTC
    CAGCAGCAGC CACTCCCCAC GGGGCCGCCC ATGCCCACTG GCCTTGCCCT GGAGCTGCCG
    ACTGCAGACC ACCGCAACAG CAGCGTCAGG AGCAAGTCCC AGTCTGAGAT GAGCCTGGAG
    GGCTTCTCGG AGGGGCGGCT GCTGTCGCCA GTGGTTGCGG CGGCGGCCCG CCAGGACCCG
    GTGGAGCTGC TGCTGCTGTC TACCCAGGAG CGGCTGGCGG CGGAGCTGCA GGCCCGGCGG
    GCACCACTGG CCACCATGGA GAGCCTCTCG GACACTGAGT CCCTGTACAG CTTCGACTCG
    CGCCGCTCCT CAGGCTTCCG CAGCATCCGG GGCTCCCCCA GCCTCCACGC TGTGCTGGAG
    CGTGATGAAG GCCACCTCTT CTACATTGAC CCTGCCATCC CCGAGGAAAA CCCATCCCTG
    AGCTCGCGCA CTGAGCTGCT GGCGGCCGAC AGCCTGTCCA AGCACTCACA GGACACGCAG
    CCCCTGGAGG CGGCCCTGGG CAGTGGCGGC GTCACTCCTG AGCGGCCCCC CAGTGCTGCG
    GCCAATGACG AGGAGGAAGA GGTTGGCGGT GGGGGTGGCG GGCCGGCCTC CCGCGGGGAG
    CTGGCGCTGC ACAATGGGCG CCTGGGGGAC TCGCCCAGTC CTCAGGTGCT GGAATTCGCC
    GAGTTCATCG ACAGCCTCTT CAACCTGGAC AGCAAGAGCA GCTCCTTCCA AGACCTCTAC
    TGCATGGTGG TGCCCGAGAG CCCCACCAGT GACTACGCTG AGGGCCCCAA GTCCCCCAGC
    CAGCAAGAGA TCCTGCTCCG TGCCCAGTTC GAGCCCAACC TGGTGCCCAA GCCCCCACGG
    CTCTTTGCCG GCTCAGCCGA CCCCTCCTCG GGCATCTCAC TCTCGCCCTC CTTCCCGCTC
    AGCTCCTCGG GTGAGCTCAT GGACCTGACG CCCACGGGCC TCAGTAGCCA GGAATGCCTG
    GCGGCTGACA AGATCCGGAC TTCTACCCCC ACTGGCCACG GAGCCAGCCC CGCCAAGCAA
    GATGAGCTGG TCATCTCAGC ACGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.